LOCUS       DAVKQL010000001        61934 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVKQL010000001 DAVKQL010000000
VERSION     DAVKQL010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 61934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61934
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.001"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(224..586)
                     /locus_tag="VW561_000001"
     CDS             complement(224..586)
                     /locus_tag="VW561_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEU9942359.1"
                     /translation="MLEVGLNFLISLFTFTFDILYPIVKVGHTDDYSHGAVKLSVSLV
                     DKDAMKKISVTVIGYFEINIDENITDILYVNGTAILYHYLRSIVSIVSAIDSSEAMLL
                     PTINVLELLDKSQPFEEE"
     gene            600..674
                     /locus_tag="VW561_000002"
                     /pseudo
     CDS             600..674
                     /locus_tag="VW561_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(946..1104)
                     /locus_tag="VW561_000003"
                     /pseudo
     CDS             complement(946..1104)
                     /locus_tag="VW561_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(1482..2171)
                     /gene="rplA"
                     /locus_tag="VW561_000004"
     CDS             complement(1482..2171)
                     /gene="rplA"
                     /locus_tag="VW561_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEU9942360.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(2379..2804)
                     /gene="rplK"
                     /locus_tag="VW561_000005"
     CDS             complement(2379..2804)
                     /gene="rplK"
                     /locus_tag="VW561_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEU9942361.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            2936..3073
                     /locus_tag="VW561_000006"
     CDS             2936..3073
                     /locus_tag="VW561_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942362.1"
                     /translation="MKIKEQTRKLAAGCSKYSFEVADKTDEVSSKHCFEVVNRTDAVS
                     R"
     gene            complement(3113..3829)
                     /locus_tag="VW561_000007"
     CDS             complement(3113..3829)
                     /locus_tag="VW561_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEU9942363.1"
                     /translation="MKKRYLVLTALLALSLAACSQEKAKNEDGTAKTEQTAKADGTVG
                     SKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKAAADRYSARPGYSEHQTGLAFD
                     VIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            complement(3832..4302)
                     /locus_tag="VW561_000008"
     CDS             complement(3832..4302)
                     /locus_tag="VW561_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU9942364.1"
                     /translation="MCLICQRIDLIKKEENPYFVKELETGYLVIGDHQYFAGYSLFLA
                     KEHVTELHHLKKETRLRFLEEMSLVQEAVAKAFAAEKMNIELLGNGDAHLHWHLFPRR
                     TGDMNGHGLKGRGPVWWVPFEEMTAETCQVKPEEIKRLVECLSSEVDKLLEIKE"
     gene            complement(4304..5341)
                     /locus_tag="VW561_000009"
     CDS             complement(4304..5341)
                     /locus_tag="VW561_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEU9942365.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGEKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDPRTVVTDTGFIKSRHLDDKVSAAILLNLLRIYMEEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVTLA
                     KNQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYLKSGLVD"
     gene            complement(5439..6764)
                     /gene="brnQ"
                     /locus_tag="VW561_000010"
     CDS             complement(5439..6764)
                     /gene="brnQ"
                     /locus_tag="VW561_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEU9942366.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKVSLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(6935..7027)
                     /locus_tag="VW561_000011"
     CDS             complement(6935..7027)
                     /locus_tag="VW561_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9942367.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            complement(7070..7222)
                     /locus_tag="VW561_000012"
     CDS             complement(7070..7222)
                     /locus_tag="VW561_000012"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9942368.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLECAHNLPLSQ
                     LADSYD"
     gene            complement(7382..7960)
                     /locus_tag="VW561_000013"
     CDS             complement(7382..7960)
                     /locus_tag="VW561_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEU9942369.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            complement(8014..9414)
                     /gene="pepV"
                     /locus_tag="VW561_000014"
     CDS             complement(8014..9414)
                     /gene="pepV"
                     /locus_tag="VW561_000014"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEU9942370.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVVSVSLSEHGHTPHYVPMEDPLV
                     QTLLNIYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(9427..10032)
                     /locus_tag="VW561_000015"
     CDS             complement(9427..10032)
                     /locus_tag="VW561_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU9942371.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDDKLEPSYRLPVDEIIEKR"
     gene            10203..11003
                     /locus_tag="VW561_000016"
     CDS             10203..11003
                     /locus_tag="VW561_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9942372.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKESSSQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTYLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATIDFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSTDSPSN
                     YIVFSSDQKEFKEKFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            complement(11185..12018)
                     /locus_tag="VW561_000017"
     CDS             complement(11185..12018)
                     /locus_tag="VW561_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU9942373.1"
                     /translation="MNHKIAILSDVHGNATALEAVIADAKNQGASEYWLLGDIFLPGP
                     GANDLVALLKDLPITASVRGNWDDRVLEALDGQYGLEDPQEVQLLRMTQYLMERMDPA
                     TIVWLRSLPLLEKKEIDGLRFSISHNLPDKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSQGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELLNIQFRKV
                     AYDYEAELELAKSKGLPFIEMYEELRRDDNYQGHNLELLASLIEKHGYVEDVKNFFDF
                     L"
     gene            complement(12008..13852)
                     /gene="uvrC"
                     /locus_tag="VW561_000018"
     CDS             complement(12008..13852)
                     /gene="uvrC"
                     /locus_tag="VW561_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEU9942374.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEAYFKSMAQEVSDFLKGQDDKIIDDLKSKMAVAAQSMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDSKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     SVDEIVEVGVPRVVAEAVQRKLNPQGEALPQVAEERVDYQTEGNHNEP"
     gene            complement(13892..14593)
                     /locus_tag="VW561_000019"
     CDS             complement(13892..14593)
                     /locus_tag="VW561_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEU9942375.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            complement(14672..15904)
                     /gene="murN"
                     /locus_tag="VW561_000020"
     CDS             complement(14672..15904)
                     /gene="murN"
                     /locus_tag="VW561_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEU9942376.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNSIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(15909..17129)
                     /gene="murM"
                     /locus_tag="VW561_000021"
     CDS             complement(15909..17129)
                     /gene="murM"
                     /locus_tag="VW561_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEU9942377.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFVIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKELEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRISKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(17241..18020)
                     /locus_tag="VW561_000022"
     CDS             complement(17241..18020)
                     /locus_tag="VW561_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEU9942378.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALATNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIKDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(18089..19750)
                     /locus_tag="VW561_000023"
     CDS             complement(18089..19750)
                     /locus_tag="VW561_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU9942379.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPEHIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(19925..22147)
                     /gene="recJ"
                     /locus_tag="VW561_000024"
     CDS             complement(19925..22147)
                     /gene="recJ"
                     /locus_tag="VW561_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEU9942380.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEERENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GGASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAIVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            22424..23182
                     /locus_tag="VW561_000025"
     CDS             22424..23182
                     /locus_tag="VW561_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9942381.1"
                     /translation="MALVEFKNVEKYYGDYHALRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLEAVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKTVLENVT
                     LAPIKVLGIDKKEAEKTAKKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLARDGMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSEPRAQQFLSKIINHESDKVK"
     gene            23193..23987
                     /locus_tag="VW561_000026"
     CDS             23193..23987
                     /locus_tag="VW561_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9942382.1"
                     /translation="MKKKFFLSALLISLFGLAAAKPVQADTSIADIQKRGELVVGVKQ
                     DVPNFGYKDPKTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQGDMDIA
                     TFTITDERKKLYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITE
                     LGKKKGLKFKFVELGSYPELITSLHAHRIDAFSVDRSILSGYTSKRTALLDDSFKLSD
                     YGIVTKKSNTEFNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            24000..24677
                     /locus_tag="VW561_000027"
     CDS             24000..24677
                     /locus_tag="VW561_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9942383.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHTIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQTFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            24687..25346
                     /locus_tag="VW561_000028"
     CDS             24687..25346
                     /locus_tag="VW561_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9942384.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNSVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            25648..26838
                     /locus_tag="VW561_000029"
     CDS             25648..26838
                     /locus_tag="VW561_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEU9942385.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTNNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(27159..28040)
                     /locus_tag="VW561_000030"
     CDS             complement(27159..28040)
                     /locus_tag="VW561_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEU9942386.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(28181..29509)
                     /gene="vncS"
                     /locus_tag="VW561_000031"
     CDS             complement(28181..29509)
                     /gene="vncS"
                     /locus_tag="VW561_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEU9942387.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIDFPKVVQD"
     gene            complement(29506..30162)
                     /gene="vncR"
                     /locus_tag="VW561_000032"
     CDS             complement(29506..30162)
                     /gene="vncR"
                     /locus_tag="VW561_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEU9942388.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASIAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(30211..30294)
                     /gene="pep27"
                     /locus_tag="VW561_000033"
     CDS             complement(30211..30294)
                     /gene="pep27"
                     /locus_tag="VW561_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEU9942389.1"
                     /translation="MRKEFHNVLSSGQLLTDKRPARDYNRK"
     gene            complement(30474..31853)
                     /gene="vex3"
                     /locus_tag="VW561_000034"
     CDS             complement(30474..31853)
                     /gene="vex3"
                     /locus_tag="VW561_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEU9942390.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(31905..32552)
                     /gene="vex2"
                     /locus_tag="VW561_000035"
     CDS             complement(31905..32552)
                     /gene="vex2"
                     /locus_tag="VW561_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEU9942391.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQINYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(32565..33842)
                     /locus_tag="VW561_000036"
     CDS             complement(32565..33842)
                     /locus_tag="VW561_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9942392.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGNSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
     gene            complement(33979..34263)
                     /locus_tag="VW561_000037"
                     /pseudo
     CDS             complement(33979..34263)
                     /locus_tag="VW561_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(34556..35005)
                     /locus_tag="VW561_000038"
                     /pseudo
     CDS             complement(34556..35005)
                     /locus_tag="VW561_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817843.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(35247..36131)
                     /locus_tag="VW561_000039"
     CDS             complement(35247..36131)
                     /locus_tag="VW561_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEU9942393.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVVLLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDMIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            complement(36135..36521)
                     /locus_tag="VW561_000040"
     CDS             complement(36135..36521)
                     /locus_tag="VW561_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEU9942394.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            complement(36514..37857)
                     /locus_tag="VW561_000041"
     CDS             complement(36514..37857)
                     /locus_tag="VW561_000041"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU9942395.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLDLGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            complement(37939..38823)
                     /locus_tag="VW561_000042"
     CDS             complement(37939..38823)
                     /locus_tag="VW561_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9942396.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            complement(38835..39452)
                     /gene="cysE"
                     /locus_tag="VW561_000043"
     CDS             complement(38835..39452)
                     /gene="cysE"
                     /locus_tag="VW561_000043"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEU9942397.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KHGFKLLARMYSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            complement(39468..41681)
                     /gene="pnp"
                     /locus_tag="VW561_000044"
     CDS             complement(39468..41681)
                     /gene="pnp"
                     /locus_tag="VW561_000044"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEU9942398.1"
                     /translation="MAKQVFQTTVAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQVVKDQVT
                     AVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDLEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHQKDHKPKKEFTETPKDSE"
     gene            complement(42368..43234)
                     /gene="metF"
                     /locus_tag="VW561_000045"
     CDS             complement(42368..43234)
                     /gene="metF"
                     /locus_tag="VW561_000045"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEU9942399.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNDNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQTPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            complement(43298..45547)
                     /gene="metE"
                     /locus_tag="VW561_000046"
     CDS             complement(43298..45547)
                     /gene="metE"
                     /locus_tag="VW561_000046"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEU9942400.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVAAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFCLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            complement(45808..46074)
                     /locus_tag="VW561_000047"
     CDS             complement(45808..46074)
                     /locus_tag="VW561_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9942401.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGKTSPQLDTVLKVLASLGKTLAIVPLEQGKS"
     gene            complement(46200..46603)
                     /locus_tag="VW561_000048"
                     /pseudo
     CDS             complement(46200..46603)
                     /locus_tag="VW561_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            46759..47760
                     /locus_tag="VW561_000049"
     CDS             46759..47760
                     /locus_tag="VW561_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU9942402.1"
                     /translation="MIKTFLSALSVILFSIPIITYSFFPSSNLNIWLSTQPILAQIYA
                     FPLATATMAAILSFLFFFLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NALPPFERGTWNSQSPKLFNATIDHILLPKNHYYVRDLDIVSFQNSDHRCIFTEITF"
     gene            complement(47828..50233)
                     /locus_tag="VW561_000050"
     CDS             complement(47828..50233)
                     /locus_tag="VW561_000050"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEU9942403.1"
                     /translation="MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEEERQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPENAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVSIGTDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKS
                     SRVVLEAAVFNGKSIRKTSGHLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VSVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTVTQKLRRQVRTIAE
                     GAGLTEIITYALTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFTRLGLQVTYTATSEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSAIEGALQPAVPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNA
                     EVR"
     gene            complement(50310..50819)
                     /locus_tag="VW561_000051"
     CDS             complement(50310..50819)
                     /locus_tag="VW561_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9942404.1"
                     /translation="MIRKVEMADVEVLAKIAKQTFRETFAYDNTEEQLQEYFEEAYSL
                     KTVSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            complement(50819..51865)
                     /gene="pheS"
                     /locus_tag="VW561_000052"
     CDS             complement(50819..51865)
                     /gene="pheS"
                     /locus_tag="VW561_000052"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEU9942405.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFNQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            complement(52457..53871)
                     /locus_tag="VW561_000053"
                     /pseudo
     CDS             complement(52457..53871)
                     /locus_tag="VW561_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            complement(53884..55722)
                     /locus_tag="VW561_000054"
     CDS             complement(53884..55722)
                     /locus_tag="VW561_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9942406.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGAPVTPASYGSSVVPILIMV
                     WLMKYIEKMIAKLTPAVTKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLILPNNQTIEHSKELLVIL"
     gene            complement(55740..56579)
                     /locus_tag="VW561_000055"
     CDS             complement(55740..56579)
                     /locus_tag="VW561_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEU9942407.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKIGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGVIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
     gene            complement(56880..59455)
                     /locus_tag="VW561_000056"
                     /pseudo
     CDS             complement(56880..59455)
                     /locus_tag="VW561_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442363.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            complement(59952..60755)
                     /locus_tag="VW561_000057"
     CDS             complement(59952..60755)
                     /locus_tag="VW561_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEU9942408.1"
                     /translation="MQPTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATVYHHTVDASTEEADLVSLRIVELLSRRGFRFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNIPFQQHSKY
                     FRDALVLDNAKILQRRPEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            complement(61135..>61934)
                     /locus_tag="VW561_000058"
     CDS             complement(61135..>61934)
                     /locus_tag="VW561_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9942409.1"
                     /translation="IKDFPREAVKLLVKNGEKVDVIENIDYQDAFFELGDVYHSRKMI
                     YVEDRLAKYILEFVITHSGSENLKQNLVVRYIPGGANQIICNNILNSSYLDSDNHYFW
                     LDGDQNTNVSESNNLMNYLENGVVISDKIPESDNKNLDDIIKLITGCPIKFNVSGNKG
                     QKNNIELIAKQRSFIDYWAKYVSYLPFPTPEFFLANLCNSVDREGYDFSKDGNGKEYF
                     RKKTQVALGIENITSEDIFQEQRRAVSKIQPESSMFQCIKEKLEALF"
BASE COUNT        18016 a        13849 c        11175 g        18894 t
ORIGIN      
        1 ataaactaac ctcctcatta ttagcaagga ttctactgat tataacctaa ttcttagact
       61 acttcaagac taactgtcca ataataatct acagataaat catccactct taataaaatt
      121 ttattcaatc catctactca ttcccaaaag gttgctgtat aatgcaagac atttttctta
      181 ctgattttac ttttccttac cctcaattta gtgagctaat aaattattct tcttcaaaag
      241 gttgagattt atctagtaac tctaaaacat taatggtagg tagcaacatt gcttcactgc
      301 tatcaattgc cgaaactatt gaaacaatag aacgtaaata atgataaaga atagcagtac
      361 catttacgta tagaatatcg gtgatatttt catctatatt tatttcaaaa tatcctatta
      421 cagttacaga tatttttttc atggcatctt tgtcaactaa agaaacacta agttttacgg
      481 ctccatgact ataatcatct gtatgaccaa ccttaactat gggatataat atatcgaagg
      541 taaatgtgaa caaactgatt aggaaattta aaccaacttc taacaatctg ttatactcaa
      601 tgaaaaccaa agagcaaact aggaaactag cctcaggttg ctcaaaacac tgttttgagg
      661 ttgtagatgg aactgacgta gtcagctcaa aacactgttt tgaggttgtg gatagaactg
      721 acgaagtcag ctcaaaacac tgttttgagg ttgtggatag aactgacgaa gtcagtaacc
      781 atacgcacgg caaggcgacg ttgacgtggt ttgaagagat tttcgaagag tattagaaag
      841 ctcgatggac tattccagat tcattccact agatttcgaa atcgagtagg ggataatctc
      901 tagcccctct cacaccaccg tacgtgccgt tcggcatacg gcagttcaac taacttttaa
      961 cgcatgtcgt tcaaggtaat aatccaaaca cgaaaccagt tcacgttttt caaggactgg
     1021 ttttgatata gcacgtttaa gtaccgactt ctgagctact aattgatagt ggtcgcccca
     1081 gccagatacc ttatccgcta tataggcctt attatcacgt tcctgttcgt agagccacga
     1141 tttcgctatc ccttcctctg acgattctta tttccagcgt cagacttaaa acggtctatc
     1201 cccagaccgt tttaatccgc tacctcacga tagtcagact tgggaatcgc tattgggttc
     1261 accggtagca ggtgcccacc gaggacttac acctcaaata tataataaaa tgaaataaga
     1321 acaggacgaa tcgatcagga cagtcaaatc aatttctaac aatgttttag aagtagatgt
     1381 gtactattct agtttcaatc tactatacaa catgcccgtc gtacatcaaa aaaagttgag
     1441 acatagcgtc ttcaactttt ttacattact ttaactactg attaaagtga gtttacgtca
     1501 actttgatac caacaccttg agtagttgtg atagtcaagt ttgttacgta agttccttta
     1561 gctgtagctg gttttgcttt ttggattgtt tcgttgaaag ctttaaagtt ttcaaccaat
     1621 ttttcagctt caaatgatac tttaccgatg attgcttgaa cgttacctgc acggtcagca
     1681 cggtaagtga ttttaccacc tttagactct tcaactgctt tagcaacatc cattgttaca
     1741 gtaccagttt tagggtttgg catcaagtta cgtggtccaa ggacacgtcc aagacgtcca
     1801 acaagagcca tcatatcagg tgtagcgata actacgtcga agtccaacca accgtcgttg
     1861 attttagcaa caaggtcatc ttcaccaaca aagtctgcac cagcagcttt tgcttcttca
     1921 gcttttgcac cacgtgcgaa aacaagaaca cgtgaagttt taccagtacc gtttggcaat
     1981 accattgctc cacggatttg ttggtcagct tttttaacgt cgatgttcaa gttgtaagca
     2041 acttctacag ttgcgtcaaa ttttgcaaag ttagtttctt ttgcaagtgc tacagcttct
     2101 tctacactgt atgcttttgt gctgtcgatt ttctcaagag cagcacgaag ttgtttgctt
     2161 tttttagcca ttttctattc tccttgtaag tggttcaatc gattttcatc tcccacgtca
     2221 cttctcgaaa tgatgaagtc ttgcgggttt tcagtctact atttttctct tcctacggcc
     2281 ttaagctccc ttgctgtacc caagtacaag gctcagtcac ttgcctagta tgaaaagcaa
     2341 ctaatagact gctgtgagat tttctactgt gttattgatt agtcaacaac agtgaatccc
     2401 atagaacgag cagtaccttc gatcatacgc attgcagact ctacgtttgc tgcgttcaaa
     2461 tctggcatct tagtttctgc aatttcttgt acttgtgcac gagtaactgt agcaacttta
     2521 gttttattag gtgtacctga tcctttttca acacctgcag cttttttcaa aagaacagca
     2581 gctggtggtg ttttcgtaac gaaagtaaat gatttatctt cgtaaactga gataacaact
     2641 ggaatgatca taccagcttg gtcagctgta cgagcgttga actcttttgt gaatcccatg
     2701 atgttgatac cagcttgacc aagagcaggt ccaaccggtg gagctggtgt agctttacca
     2761 gcagggattt gcaattttac aagtttttcg acttttttag ccatttttaa atcctccttt
     2821 gtggttttgg cggtaattag agatttttac ctcccacaag tatgtttttc acatacccat
     2881 ccattataca ctatttatct ggaattgcaa gagaaaagtt tattttttgt actcaatgaa
     2941 aatcaaagag caaactagga agctagccgc aggctgctca aagtacagct ttgaggttgc
     3001 agataaaact gacgaagtca gctcaaaaca ctgttttgag gttgtgaata gaaccgacgc
     3061 agtcagtagg taaggcgacg ctgacgtggt ttgaagagat tttcgaagag tattaatcga
     3121 cgtagtctcc gccttcaaag ccatagtatt cttccaaact gagaccactt gcagcaattt
     3181 ctttagcttc ttttcctaca taacgcaggt gccattcttc agccatatag cctgtttcct
     3241 tttccttgcc tttgagataa cggacaacaa agccataatc agctgcatga tccaagagcc
     3301 attgggctgc tttttcttct gtcaccaaat caccatcagt cccaatcaca tcaaaggcca
     3361 agcctgtctg gtgttcgcta tagccaggac gggcagagta acggtcagct gctgcctttc
     3421 catcttggtt gacataatct tgatagagct tggtctgagt ttcataactt ctaaaaccac
     3481 tgtaatgatc actaataggg aaacctgcct cttgcatcgc tttgatgagt ttgaccaact
     3541 cagccttggc tgttggattt tccccgggat tatagtcttt cgacaaagga taatgtttat
     3601 tggctacgat gatttcgtcg tattttcctt ggatgctgta gtaatcacct ttattgacca
     3661 cttctgcttt tttctgggca gccccttgag acttactgcc gactgtccca tcagctttgg
     3721 ctgtctgttc tgtcttagct gtgccatctt cattttttgc tttttcttgt gaacaagctg
     3781 ctagactcaa ggctagcaag gctgtcaaga caaggtatct ttttttcatt tctactcctt
     3841 tatttctaat agtttatcta cttctgacga taaacattcg actaatcttt taatctcttc
     3901 cggttttact tggcaagttt ctgctgtcat ttcttcaaag ggaacccacc aaactggccc
     3961 acgaccttta agaccgtggc cattcatatc acctgtccgt cgtggaaaca gatgccaatg
     4021 aagatgggca tcgccatttc ctagcagttc gatattcatt ttctcagcag caaatgcctt
     4081 ggcaactgcc tcttggacta gactcatttc ttcgagaaaa cggagtcttg tttccttttt
     4141 caaatggtgc aattcggtaa cgtgttcctt agctagaaaa agactatatc ctgcaaaata
     4201 ctggtggtct ccaatcacaa gatagcctgt ttccaactct ttgacaaagt aaggattttc
     4261 ttccttcttg atgaggtcaa ttctctggca aatcaggcac atattagtct accaacccac
     4321 tcttaagata agcatcaacc atacgctctg ttgcgaccac cgagtcaata tgagtacgct
     4381 cataagaatg actagactcg atacccgcac caagaagagc gtgtttaact tctgcacctg
     4441 cagacatagc cgctgaagcg tccgaaccat aaaatggata gatatccagc ttgaatggaa
     4501 tatcttggtt tttagccaag gtcaccaaat gttgacggaa gtcatagtga taaggacctg
     4561 aagcatcctt gacacagatg gacactgtgt actcatctgt ttgctgatca tcccccatag
     4621 ctcccatatc cacagccaga tattcgacta cttgagcagg aatattagaa ttagcaccat
     4681 gccccacttc ttcaaagact gaaaaggcaa aatgggttgt tactggcaac tcaatcttct
     4741 cttccatata aatacgaagc aggttaagca aaatcgctgc gctgaccttg tcatccaaat
     4801 gacgagactt gataaaacct gtatcagtca caacagttcg tggatcgaag ctgataaaat
     4861 caccgacctc aatcccaaga gcacgagttt ctttttcatt gcttactttt acatccaaac
     4921 gcacttccat attgtcctgc gtgcgttctg cagttcctgc atccttgtag acatggcaag
     4981 aagtttggtg gatgaggatg gtgcctgata ctttttcacc tgtgctagcc acatgaacgg
     5041 tacagttttc accttcaatc atgttccaag gaaaaccacc gatacggtcc aatttaagac
     5101 gaccatctgg tttgacagca cgaacaatag cacccagcgt atccacatga gcagtcacat
     5161 agcgatgttg ctcatcattt tgacctttga tggtcacatt gacaccaccc ttagctgtgc
     5221 gaactggctg gtaaccaaaa ccttctagag tcttgactaa ataattcgaa atttcacgag
     5281 taaagcccgt tggcgatgcg atggctgtca gttctttgat atattctact gtttgattca
     5341 tttcttcacc tctttcgcta cctattataa cacatttatc atcggataaa aaaagaaaat
     5401 cactcctgta gacttggcta caaaagtgat ttaagtgatt attccatttc aaaaacatcg
     5461 ctttcttgct tgtttggtag aaccaatgag agcaagattc caacaatggc tggcaccaac
     5521 catgggagag ataccttggc aaaaggaaga acgctaacaa gatttgcaag aaactcaacc
     5581 ttaaacgagc ttcctagtac gcttgcaatg gcaataactg taaccacagc aattgtcaac
     5641 tgcatacctg gttttgaaag ggcaacaaat ttgttgacaa tgacaatcat aacgatagca
     5701 atcgtgattg ggtacaagat aaccaatact ggaattgagt acttgataat cgcatcaaga
     5761 cccaaattgg caatagcaaa tccaatcaag gtaaaggctg tcgcataaac cttgtagctg
     5821 atttgtggga agcgttcatt aaagaactca gctgttgaca caatcaaacc aacagtcgtt
     5881 gtgaagcagg ttacggtaac catagctgca aggaagagtt gagctgttga gccaaagatt
     5941 tcttgagtgg cttgtgacaa gatgtaaaca cctggtgttc cacccttcat cgcttcagct
     6001 ggtactggga aatgatttcc aagaaaacct aaaccgatgt aaagagcgct gaaggcaagg
     6061 gcaacaacga taccaacaac ccaaatagtt gaaatgtatt ctttcttact tgaaaatcca
     6121 agttgtttca aggtttgaac tgcgattacg ctaaaggcca ctgaggcaag ggcgtccaag
     6181 gtattataac cttctaggaa acctgtacca aaggcagaag cttgataagc agctgaagca
     6241 gcttgaggac ttgttccacc atatttgata gctcccagaa cgaccaagat aacaatcaaa
     6301 attgcaaaga ctggcgttaa aatacgtcca atgcggtcta agatttttga tggattaagc
     6361 gaaatcaaat aggctgccgc aaaatacaga accgtaaata caatcaagcc aagtccttta
     6421 tttgcatccg acaaaagggg gctaatccct acttcgtaag ctgttgtagc agtacgtggg
     6481 gtagcaaaga atggaccgat tgacaagtaa agaactgaga ggtaaagagt cgcaaaccaa
     6541 ggcgctatct tcgttgaaat ctcgtagata tatcctttag gatttagcgt tccaataata
     6601 agggtcaaga cggcgatacc aacgcctgaa aagacaaaac ctgcgatggc aggaagaaaa
     6661 tgttctccag atagagcacc tagagaaggc ggaaaaatca agttccccgc accaaaaaat
     6721 attccaaaca ggagcaaacc tgttagggta ccttttttag ccataaaaat ctccttcata
     6781 tttataaaat actgacctag tataccacga ttaggagtct gaaaaaagca tcacgaggca
     6841 aatattaaat gttttcaatt ttcttaaaaa taagaattat ctaaaaataa aactctccta
     6901 cccagtccac accaagtagg agaaatgcaa aattttaaag ttcttcaaag gctaacatgc
     6961 caccctggac attgataaca ttataacctt gttctaacag gaattggcaa gcacgcgccg
     7021 atctcattcc agatttgcaa ataatataat gcaagcgatc tttgtccaat taatcataac
     7081 tatcagccaa ttgactaagc ggtaggttgt gggcacattc taaatgaaga gctgcaaact
     7141 catccacttc tctcacatcc actagagaaa gctggtcgtt ttggtaaagc tggtaaaatg
     7201 cgtcaaaggc tatttctttc attctttttc ttctaatact cttcgaaaat ctcttcaaac
     7261 cacgtcagcg tcgccttacc gtgcgtatgt taccgacttc gtcagtttta tctgcaacct
     7321 caaagctgta ctttgagcag cctacgacta gcttcctagt ttgctctttg attttcattg
     7381 actataaaat ggttttaatt ctttttttca aatctggcac tacttctgcc tcaaaccaag
     7441 gatttttggc catccagatt tgatttcgtg gtgatgggtg aactagcgga aaataggctg
     7501 gcagatagtc tttatagtgt ttcaccctct ccgttacctt cccactgatt ttctcctgta
     7561 aatagtaggc ttgggcatat tgcccaatca agagggttaa ctgaatatca ggcaattcct
     7621 gtaagagctg cggatgccat ttttctgcaa aacctgtacg aggcggaaga tcacccgact
     7681 tgccatgtcc tggaaagtag aaatccatag gcaaaacagc aaaataacct gaattgtaaa
     7741 aggtatcttc atccacacct agccagtccc gcaagcggtc accactttta tctttccagt
     7801 aaaggcctgc ttcttgagtt ttaagtcccg gagcctgacc gatgatattg atgcgagcag
     7861 tttttggcgc tgcaaagaga ggctcaatgc cacgctctgt atagctggca ttctgtgaat
     7921 ccgccatgat agcctgtttg attctttcga tttgagacat ctactttccc tccaaaaaga
     7981 agtccaagca taagatctca gacttctatc gttttatttg atcaattcgt aaatagcttc
     8041 ggcataaatt gctgctgctc ggaaaagatc atccaaggcg ataaattcat tggcttggtg
     8101 catggtatca atcgagtctg ggaacatagc accgtaggca actccgcgtt ctagcaagcg
     8161 accaaaggtt ccaccaccga tgacttgttc atgaccttta aggccagttt gtttttcata
     8221 gatattcaac aaggtttgca caagtggatc ttccattggc acatagtgag gcgtgtgacc
     8281 gtgttcagac agactaacag aaacaactgg caagttttca aggattgact tgatttgttc
     8341 tggacttgtt ccttttggat agcggatgtt gagggcaatg gtattatcag cacttgtttc
     8401 atcgaagcgg aagacgccgg cattcataga aagagcaccc atcttttcat ccacatgagc
     8461 aatcttaaga ttttcaccct catgatcgtt caagagaatt ttacctgcga tgtcaaggta
     8521 gtctttggct ggaccagcaa agccaaactg gctgaggaag agggcaaggt aagttgcgcc
     8581 attgacacct gaagcaggca tagcaccgtg ggctgattta ccaatgatcg tcaccttgta
     8641 tttgccagct tcttcttgga gttctcctct aagtttgtgt tctgcaacaa aggcatctag
     8701 tttagcttgc aagtcagcca aatcacctga aacgactgct gttgctgatt ctggtaccat
     8761 attttcacgt aaaccacctg taaagctgtg aagacgggca gcacctgtat tttctcctgc
     8821 aaagtggagg tattccgtga tatttccttt ttcaccattg atgattggaa attcagcatc
     8881 tggagagaaa ccgaaatctg gtttggcaag tcctacgtgc tcaaagtagt agtccatgtc
     8941 tgcccagcct gattcttcgt ctgttccaac gatgaagcga actttcttag aagttggaag
     9001 acccaattct ttgatgattt tcaaaccata gtaacaagct gttgtaggac ccttatcgtc
     9061 cgaagccccg cgcgcataaa ggcgaccatc tttgatagtt ggtgtgtaag gatctgtgtc
     9121 ccaaccgcta ccagcaggca ccacatccat atgggcaaag attccgagaa cttcttctcc
     9181 atcaccaaac tcaaaatgtc ctgcgtagtt atcgacattc ttagttgggt agccatcgcg
     9241 gtctgcgatt tcaaggaatt tctccaaggc ttttactgga ccaggtccaa atggatgctg
     9301 ggcatcagcc ttgctatcat cacgttctga gttgatttcc aaaaggctaa acaagtcagc
     9361 caagaggtct tctttgcgtt tttctacttc tgctgtaaaa tcaattgctg tcattttttc
     9421 ttctttctat cttttctcga tgatttcatc tactggcaag cggtagcttg gttccaattt
     9481 gtcgtctgtg taacccactg tgatcaagag ttctgggcgg aaacggtctt cgatttccaa
     9541 aacttcatta acttttgatt tgtcaaaacc aaggataata ttggaaccaa ttccttggtc
     9601 tgtaagagcg agaaccaagt tcatagcaac caaacctgca ttgagtgcta ggtagtcgct
     9661 gacttgttgt tcattgtagc gtgcaaattc agctggcaaa tttttcatga agtattgaag
     9721 ttgttcttct gagaaattgt tagcaccacc aactcgggca atcttacgag cgcgtttggc
     9781 aaggtctgta tctgtaaata gggcaatggt tacaggtgct gatgatacct gctcaaagtt
     9841 tgaaccataa gccaactttg ccagttcagc atttttctca cgaacaacta caaatttcca
     9901 aggctggctg ttgtgggcgc ttggcgccaa ggttgcaatt tcgatagccg tacgcacatc
     9961 ttttggatca acaggcttat cagtgaaatg cttggtcgca tgacgttttt tatttaattc
    10021 aagaaatttc ataatcgatt tccttttcta attctactgc ttccattcta ccataatttg
    10081 aaagagcttg cagggatttt tcatgattaa gtttttctat cacagacata aaagatatat
    10141 attggtttat caggaaagtt tctgataaac tagcaagtac tttacatttg aatggagaca
    10201 gtatgaaaaa atttagccta ttactagcta tcctaccatt tttggttgcc tgtgggaatc
    10261 aagctacacc caaagagtcc agctctcaaa agacaatcgt ccttgctaca gctggcgatg
    10321 tgccaccatt tgactacgaa gacaagggca atctgacagg cttcgatatc gaagtcctaa
    10381 aagcagtgga tgaaaaactc agcgattacg agattcaatt ccaaagaact gcttgggaga
    10441 gcatcttccc aggacttgat tctggtcact atcaagcagc agctaataat ttgagttaca
    10501 caaaagagcg cgctgaaaaa tacctctact cgcttccaat ttccaacaat cccctcgtcc
    10561 ttgtcagcaa caagaaaaat cctttgactt atcttgacca gatcgctggt aaaacaacac
    10621 aagaggatac cggaacttct aacgctcaat tcatcaataa ctggaatcag aaacacactg
    10681 ataatcccgc tacaattgat ttttctggtg aggatattgg taaacgaatc ctagaccttg
    10741 ctaacggaga gtttgatttc ctagtttttg acaaggtatc cgttcaaaag attatcaagg
    10801 accgtggttt agacctctca gtcgttgatt taccttctac agatagcccc agcaactata
    10861 tcgttttctc aagtgaccaa aaagagttta aagagaaatt tgataaagcg ctcaaagaac
    10921 tctatcaaga cggaaccctt gaaaaactca gcaataccta tctaggtggt tcttacctcc
    10981 cagatcaatc tcagttacaa taacactctt cgaaaatctc ttcaaaccac gtcagcttta
    11041 tctgcaacct caaagctgtg ctttgagcaa cctacggcta gcttcctagt ttgctctttg
    11101 attttcattg agtataagat atactaaaag cgattggacc agccaatcgc ttttttagtt
    11161 tgcattggct attttaggaa actcttacaa aaaatcaaaa aaattcttca catcctctac
    11221 atacccatgc ttttctatta agctggctaa taattccaga ttgtgcccct gatagttatc
    11281 gtcacgacgc agttcttcat acatttcgat aaagggaagc cccttggact tggccaattc
    11341 taactcagct tcgtaatcat aagcaacttt acgaaattgg atattgagta attccccatc
    11401 ttcaacttct atcacggcat actgggcacg gtgatttttt aacgcctccc aattaaaata
    11461 gggcatgcca atcgaccctg gattgatgat ttgttgccct tgacttccat aacgaagcaa
    11521 ctgcttgtga acatgaccat aaactgccac gtccgtttcc gcatctagca gttggtcaaa
    11581 tttctctgta tcattctcaa ctagcaagtc accaccatag tttttgtcag gtaaattatg
    11641 agagatagaa aagcgcaatc cgtcaatttc tttcttttcc agcaaaggca agcttcgtag
    11701 ccagacaatc gttgcaggat ccattcgctc catcaaatac tgtgtcatac gcaagagctg
    11761 aacttcctgt gggtcttcta agccatattg cccatctaaa gcctcaagga cacgatcatc
    11821 ccaattgcct cgaacacttg ctgtgatagg aaggtccttt agcagggcga ctaagtcatt
    11881 tgcgcctgga ccaggaagaa aaatatctcc cagaagccaa tattcactgg ccccttgatt
    11941 tttagcatct gcaatcactg cttctagcgc cgtcgcattg ccatgaacat ctgataaaat
    12001 tgcgatttta tggttcattg tggtttcctt ccgtttggta atctactctt tcttctgcta
    12061 cttgaggcaa ggcttctccc tgcgggttca actttctttg cacagcctct gcaacgactc
    12121 taggtacccc aacttcgaca atctcatcca cactggcttc cttgattttg gtcaaagact
    12181 tgaaatgcct catgagattt tgcttgcgtt taggtcccag accatcaatc ccatccaatt
    12241 gagatgagaa agaatttttg gagcgcagtt ggcggtggaa agtgatagca aagcggtgga
    12301 cctcatcttg gatgcgttgg aggaggaaaa attcctgaga attgcgagac aagtccacca
    12361 cctcaagcgg atctccaaag agcaattcat gggtttggtg cttatcattc ttttgcagcc
    12421 cagcaattgg aatatccaag cccagttcct cttggataac ctgcttagcg atattgactt
    12481 gaccttgccc cccatcaatc acaatcaaat ctggaggagt caaagcctca cgctgtactc
    12541 gaccatagcg tctgcgaatg acctctctca tgctggcata gtcgtctggt ccaacaaccg
    12601 tttttatctt gtacttacgg taatccttct tactcggttt accgttgaca aagaccacca
    12661 tagccgaaac agggctagtt cccatgatat tagagttatc gaaggactcg atacgtactg
    12721 gggtcgggat ttggagcaaa cgccctagat tttcaatagc tccttgggtc ttttcgacag
    12781 atttttctag cagattgaac ttctgctcta gactaacacg agcatttttt atggctagat
    12841 tgaccagttg ttttttctct ccacgttgag gcttaagaat cttggaatcc accaaggcct
    12901 tgacagcctc ttcgtcaata tcctgcggaa tcagtacctc attgggaact agatgagatt
    12961 tttcttgata gaattgtcct acataggtca aaaaatcctc atctggatca ttgaagtagg
    13021 ggaagagatt gacatcgcgc tcgataagct taccctgacg gacaaagaaa acctgcacac
    13081 acatccagcc cttatccaca tagtagccaa agacatcgcg attttgcaaa tctttcgcca
    13141 tgacccgttg cttggttcga agcgttccaa tagcctgaat caggtcacgg tattccgccg
    13201 cacgttcaaa ctccatactt tgtgctgcta ctgccatttt actcttgaga tcatcgatga
    13261 ttttgtcatc ctgacctttc agaaaatcag acacctcctg agccatagac ttgaaataag
    13321 cctcatcctt cttacagatg gtgtgggcca tacactggcc gatatggtaa taaaaacaga
    13381 ccttagaggg cgggttggta cattttcgaa aagggaaaat cctatctaga agtcgcttga
    13441 tttcattggc tgcccccaca tctggataag ggccaaaata aagaccaccg tcctttttga
    13501 cctgacgagt aatgataagc cgtggatagc gctcattggt gattttgatg aaaggatagg
    13561 acttgtcatc cttgagcatg atattgtact tgggcttgtt ttccttgatc aggttgattt
    13621 ctaggagaag tgcctcaata ttagactccg taacaataaa ttcaaaatcc acaatttcag
    13681 acaccagagc ctctgtcttg gtatcatgac ttccacgaaa ataggaccgt actcgattac
    13741 gcagattttt agcctttcct acatagataa tggtgccatt tttatcctta tgaatgtagc
    13801 aaccagggct ggtcggcaag agctctagtt ttgatttaat caagttgttc atagttccat
    13861 tatagcaaaa aaagtaggga aagatagtac cctactcact agtctcatat atttttcgga
    13921 ggtttgcaac atcctcttcc tggataccat aaaaggaacc tgctggttgc atgacagact
    13981 tctcatctgt atggtccaag ccaatcgcat gacctaactc atgttctgcc gtatggacaa
    14041 ggcgttcata ggagtagcca tagtatggat tggacaaata ataatgattc aaccgcaccg
    14101 ttacggacaa gaattgccct gttaagagat tagtttgact ttccgcctct cctgccacag
    14161 gagtgcctcc gtcgttcatc tccgtagccg taatatccgc cttactagac tcagtcacga
    14221 gttcaaagtt aaaagcacca gtttgattcc agttctggat tgcttctaaa taagcctctt
    14281 gaaagcgtga atccatctgc ggatccaggt aaatacgagc agaggcctgt gcccaccttc
    14341 cttcagaatg ctggtgacta tctgtctggc ttaacttagg cagttgccct gttttctccc
    14401 attggtcaat actttgttga ccaattttta ctgaccgttc tgcttgcttt agcgctcctt
    14461 gaaaatcccc gttcagatac cagagaagtc cgaaagcaag cacacataag agcacaacta
    14521 tccaaaccag acgccaaaac aaacgccaca caaaagaaaa gaaagcccct atcaaacgaa
    14581 aaagccagcg catgtctctc cacctttcta gcaaataaga attattttac taaaagaagc
    14641 taaaagaaat ctaaatactg actttttcat cttaacgtcc tactattttt ttgagtaact
    14701 ggatagcttt gtattttaaa ggcgatggat ggtaacggaa agtacccgct ttgcgtacaa
    14761 tatagccatt aaaattctgt ttaaaacgca aaacaccatc acttccatca aaaatccctt
    14821 gaatgcctag gaagttgtat ttaggtattc cacgttttat gctttccaac ataacatatt
    14881 tttgaagcag ggcaggggca tagaacttat taaactcagt gtaggaacca ctaaagagat
    14941 aagtcgtttc ctgaggcata taaacaaata aactcccagc taaaacaata tcttcttctc
    15001 catatttttc aatcaagtct cgcgcttctg cttttcgaac ttcaaacgtt tcaaattgac
    15061 tagaatattc tctcagttga ttttgttttt tctcagaatg aggattttta ctcaaatcaa
    15121 gtcgcaactt gtccaagttt tcttctagtt tactttgttc accttgcaat ttgctcatat
    15181 agtccgaaaa atttaagctt gctatgagaa actccgcttg ttctccaaaa gtatcataaa
    15241 aatgctcata atattctaaa cttttatcac tatattctct acgttcagag gtttcttttg
    15301 ttatattctt aaaaatcgat agttcttcac gttttaactt tttcaaccga atgccaaagg
    15361 tttcagcctt tttcaccaag ggtttaccct ttttgctaaa acttttaagc aaactctttt
    15421 cagttaattc agttaaatct ttatagtata accaatctgg ttctccacct gggtaacctg
    15481 ttgttaagcc atcaaattga taacctaaat cagtcaaacc ttgaataata ctttttttct
    15541 cagcatctat tgaattacct tggctatcaa aagtttgata agtttcataa ggttttacaa
    15601 gcaactctaa tacaccattt tgcttggcat attcttttaa ctctgcataa aaaactggaa
    15661 gagcatcttg ttgggtataa atcggccccg aattgagttc catatgcaga ccacccagca
    15721 tgggcaagct ataaaccaga gctgcaactt gaatttctcc ttcttgtttc aaagcaagat
    15781 aaacaattcg agcccctctt ttttctagca aatcccccat ctggacagat tgcataaagg
    15841 aacgagaaga aacctgatca gaataagtct gaaactcttc tttcgtgagt gttgttagtg
    15901 ccatatactt actttctatg tttttttctt aatgttttac ggaaatcaag agcaagtctt
    15961 aacagaggat agagaggatg agtgggcatt gtaaattcac ccaagtattc ttcaatcgtt
    16021 ggattaaatt tttccttaaa atgataaagt ccaccattga gagagttttc aacaccacct
    16081 aaattttgcc aaaccatacc tcgctcaaag gcatagcgag ccgtttcata ccatgttaaa
    16141 attggtgcat tgtaacgttt aaaatcatca tccataccag catatatatt gacagaggta
    16201 gtaccaaatt ccaaactcaa agtagccgct aaaggaactc tcgcttgacc tacatctata
    16261 tattcctgca agaaggtcaa ttcctctaac aaacgttctt tttccttctt ctgcgcttct
    16321 acttttgaaa ttcgagtcga ctcagtaaac gtctcttcca aggctctatt tttcgctaac
    16381 tgttcttcta attcttgcga acgtttagaa acatccaagg tggccaaggt gatataggcc
    16441 ttgtccttaa aattatctaa caatttttta taataggctt cattcctcaa atgaatctct
    16501 tttcgcttct cagttttttt catcaactcc gaaaatgaat ctaatagttc cagtccacca
    16561 tattgaatct caagctcttt gtttcgtgct gttcgaatag cctgttttgt tgacttggaa
    16621 agtttatctt cttcaaaatt ttccttgtat attttcgcct gaatacgagg ttgaatggtg
    16681 tctcccattt cctccgtttt tcctgaccac cttactccca tttgttgcaa actatcaata
    16741 atagccagat tttcaggaaa ttctgtcttt tcctgattga ttaaactttg agataggcaa
    16801 atacttgggt caaaagtcac aaaaaccgct ctcttactgc gagcatagga cttaatagac
    16861 tgaatgacaa aattcaagag ttctttatcc ccataatcca atataggtcc tcttgggatg
    16921 taaaacattt tatagcctag cggaagagtt ctaatcaaaa tactagctgt cgccagtaat
    16981 ttttcttccc tgtaaacacc aaacttctca tgttgccaat tagacttaac ttcctcccaa
    17041 gcactacttt gtaatacatt ggctaattca tgttctttga caaactgatc atattctaat
    17101 gtgggaatgc caatttgata acggtacatt tcctactctc tttcctccag tattttatta
    17161 ctattctact actttcgttt gaaaagtcta gtgaaaacat attttatttt agttctactc
    17221 cccctatgct gaagttcaaa ctaagtcagt ctctcttcta atttgaaatc aattggtagg
    17281 gttgttaaaa aaacttccaa ttgtttttcc cagtagtacc actcgtgagt tccagcgcta
    17341 tggctatagg tcacatcaaa acctagtttt ttgagatttt tcactgcgag attattggct
    17401 tcgtacaaga aatcctgttc gccacaccac gcccaaagtt tggtcttttt atccgatttt
    17461 ttagccagac tttcaagaga atagggacta gttgtccagt ctttaatctc tccaaaaaca
    17521 cctctccagt aggctggact tcccagattt tgactttcag gagaaaagtt ttgaaagctg
    17581 agggcacctg aaaagctagc tgcatgagaa aaacgatttg tcgcaagagc cagtttgaag
    17641 cagccgtagc ctcccataga aagaccagcg ataaaggtct tttcacgctt gctagtcata
    17701 ttagggaaga agcgtttcag aacctgtggc aattcctctg ctagagccgt gtagtagtca
    17761 aaaccatact gggtatcggt gtaccaacca ttgctggtat tgggcataac aacgatgaga
    17821 ttagttcctc gaagcaagcg ttctacattg gtccgcttaa gccaactatt atgatttcca
    17881 gacatcccgt gcaaaaggta caagacggga atatcttcac actctggttc ttccactcga
    17941 ttggcatcag ggtagaggac attcaccccc cactccatat ccaatacttg tgagtaatac
    18001 tcgattttca ttactgccat gattttctcc ttctattttt ctataagaaa aagccgaaac
    18061 tcgactttct ctctgtttat ttcaacgatt attttgcttc catgactact ggtaaaatag
    18121 ctggacgacg cttggtttgg tcaaagagat acttggtcaa attatctcga accttccctt
    18181 taagatctgc ccagtcaaag tcatctcctt gaagatactc ttctaccgtt tggttaatca
    18241 attctgaact ttcacggaga atatcgcgac tcttcttgag ataaacaaat ccgcgcgtat
    18301 gaacacgagc cctagccaca attttcttct cacgacggtt gactgtaata gccacgatga
    18361 aaattccatc ctctgacaag accttacggt cacgaagaac aacatttcca acatcaccaa
    18421 tggcattccc atcaatcaag atatctcctg ctgaaaccga tccagctgga acaaagtctc
    18481 cattctcgta agccatggtc gtcccctttt taggaatgaa gatgtgttct ggcaacatcc
    18541 caactgccat ggcagcctta gcgtgagcat ccaactcacg atactcccct tggacaggga
    18601 agaggtactt aggttgcaaa agattgatca tcagctgcaa atcacgcaca tttccgtgcc
    18661 ctgatacatg taaactttgg gtaatcaatt tgacaacccc acctgcctga taaatcatat
    18721 tttccacacg cgcaacaaag gcttctttag caatagacgg agccgtagca atatagacca
    18781 ggtccccatc cttgatttct acataacgat ggcgaccaat cgacatctta cgaagtccat
    18841 tgataggttc acccatacga cctgtctcaa gaataatcaa ctcatggtct tcaaagcgag
    18901 acatatcttt aggcttaatc aagagaattt cgttggctaa agacaacttc ttaagacgaa
    18961 tcgctgtgcg gacgatattt tcaatatcaa atcctgtcaa gacgatacgt cgacctgttt
    19021 tatccgcagc gtcaaaaatc tgctgaatac gagaaagatt actggaaaca gctgcaacga
    19081 tgatacgacc ttcccagtca gcaatagttt gggtaatttc atccctaact tcactttcac
    19141 tagccacctg aatattgctg tctgcattgg ccgaatcact gaggagagcc aggacgccgt
    19201 cacgaccaat ctctgccaaa cgagcaaaat cagttgcata agattcacta gccgtttggt
    19261 caaatttgaa gtcacctgta taaacgatgc ttccttccga tgtcttcaag acaattccca
    19321 gactctctgg aacggagtaa gtcgtaggga agaaggaaac cactgtccca ccaaaatcaa
    19381 tctccgtatt ctcatcaatg acatggaaat cattaaattt cttaacggca tcatttcctt
    19441 tgacaaagag ctttgccaac tcaatggtca actcagaccc aaatacagga actttagcct
    19501 ctgccaagag ataaggtaga gcaccaatgg catccgcatg cccgtgggtc aagaaaaccc
    19561 cagcaatacg gtcgctattt tcaaaaaggt aatccatgtt tggaatcacc acatcgaccc
    19621 ctaattgttc attttcagga tattttaacc ctacattcaa aacaaaaatg gactctccaa
    19681 tttcagcaat gtacatattt tttccattct cacgcacacc accaagtgtt gttaaactga
    19741 tattactcat ttttcctcca aaatcttagt ttatcttgat tatagggcaa ctctaccctc
    19801 ttattctaaa agttctaata ctttcagccg aatcttttcc taccattata ccattttttt
    19861 gatgattttc aaaatgaagg tctgttttca aaaaagagtt gatttcccaa ctctttccta
    19921 acttctactc tttttccatc aaaaaatcat aaatttcttg caccgtaccc agcgccatca
    19981 tttcttggtc tttaacggtt tgtttgagat tttggtaaat ttgactttct cctatctccc
    20041 gctttggcgc ctctttattg actgtcatca caccatcttt tatcgtcaca aagcctagtt
    20101 cttcaaatac ttgaatcatc ttgaccagca agatttgttg aatattaaga tatgcagcca
    20161 aatctttcag cttgtagcga atatcaaact ctgggaactg gtaaatagtc ttgtacaatt
    20221 tggcaaactg atctctagtc ccataacctg tcagataata ggccttgtca atatcatttt
    20281 tgaaatagac agcagagaaa tgctgttcct gaaaaatggt cttcagctga gtaatatcct
    20341 ctggaatgtt ttttacgaca atagcttcac tagccgcaag atttggcagt tctccaggaa
    20401 aatccaagac tggaacacct tctggcaaga ctgcattttt tccacgaatg ttaaaaagtt
    20461 gaacaccttc cactcgcgca tccaccatca tcaactggag ggcagtttgg ccattccatt
    20521 ggttgacaga caatttaacc gctaactcta gattcttggt ttgagaaaac tctgtcgccc
    20581 atctgccttg accaaaggct accacttcaa aactcgcccc acccttggaa attttcagct
    20641 ttagatgggc attacctgcc cccatagtac gagcactttc gacctgaaaa ttcttgatat
    20701 aaaaaatagg tttctgatta tccattccaa aaggagctaa acgttcaaaa cttttgaccg
    20761 tttccaagct aagtgcctcc aaatccaact cttcatctag gtttaactta ttcttgccac
    20821 cagcatctgc acctttttca cgaacataat cttccaaaac ctgagataaa tctgagagtt
    20881 gctcaacttc cagcgtcata cccgctgcac ctgcatgacc tccaaaggcg atgaagaggt
    20941 ctcgatgggg atccagagct tcaaaaatat cgaccgcttc cacactacga gcactgccct
    21001 tggcacgacc gtcttctata ttaagaacaa tgactgtctg tcccaattct tccaataaac
    21061 gaccagccac gattcctaga accccaggat tccagccttc cttggccaag acctgaacct
    21121 tcttctcagg atccaccatg gtcttggctt cttcatagat agactgaacg atttccttgc
    21181 gctcttcgtt tttctggtga atcataaggg caatctcatg cgcttcctca tcatcaaatc
    21241 cagtcaacaa atcaatggca ggattgggat catccaagcg acccaaggca ttcaaacgag
    21301 gagcaatctg gaaaccaacc gtttcttctg ttacttcgtt ggcagcaatc ccagccatgt
    21361 ccagcatttc ttgcagacca atgcgctggg tatgacccaa catttccaga ccatattgaa
    21421 ctaagatacg attttcatcc gtcagactca ccatatctgc aatagttcca atagcgacca
    21481 aatcaagcaa ttccacttgc acttcttcta acagggcaca agccaacttg aaagcaactc
    21541 cacaaccagc caaatattta aaaggataat ccgcatctgg atgttcagga tggacaatag
    21601 cataagcatc tggcagggtt tcaggcatgg aatgatggtc tgtcacaatg acatctactc
    21661 ccatagactg agccaatgca atagcctcat gaccagcaac cccattgtcc accgtcacaa
    21721 tcaaggaaat tccttcttgc tcgataaagt atttataaac actagcatta gggccatagc
    21781 catcggtaaa acgatttggc aggtaaactc ggcactccgc accaagttgt tccaaacttt
    21841 ccttcacaat agaagccgaa gtcatgccat ccgcatcgta gtctccataa acgagaatat
    21901 tttccctttc ttcaatagcc tgacgaatcc gctccactgc cttgtccata tcatggagca
    21961 gataagcatc atgtaagtcc tccaaggaag gttctaaaaa cttcttcaga ctttcttggt
    22021 cctgaatccc tctctcaaac aataaccgag ccacctcagg acccagtcca gccttcttgg
    22081 ctatctttgt aaaatccgca tcttctacct gcagggcaaa ctgccattca taagtaggtg
    22141 ttatcaaaaa gacatccact ccttctaaga attctattgc ttcattataa catgatttag
    22201 acgttttctc tatccagatt gcttttttat aacatttcat agtatattga aactagaata
    22261 gtacacctct acttctaaaa tattgttaga aatcgatttg actgtcccga tcgatttttc
    22321 ctgttcttat ttcactatag tgaatttttt cagatatggt ttgacaagaa cattctttta
    22381 ttgcagaatt gtgttataaa atctaacata aaggagttct ctcatggctt tagtagaatt
    22441 taaaaacgtc gaaaaatatt acggagacta ccacgcactc cgcaacatca atctccgttt
    22501 tgaaaaagga caagttgttg tcctgcttgg accttctggc tctgggaagt ccactcttat
    22561 ccgtacgatc aatggtttag aggctgttga caaaggaagt ctcctagtca atgggcacca
    22621 agttgctggt gccagccaga aagatttggt acctcttcgc aaggaagtcg gcatggtttt
    22681 tcaacatttt aacctttatc cacacaaaac agtgttagaa aacgtgacac ttgcacccat
    22741 taaagttcta ggaattgata aaaaagaagc tgaaaaaacc gccaaaaaat atctggaatt
    22801 tgtaaatatg tgggacaaga aagattccta tcccgccatg ctatctggtg gacaaaaaca
    22861 gcggatcgcc atcgctcgtg gtcttgctat gcatccggaa ctcctcctct ttgatgaacc
    22921 aacatctgct cttgatcctg agactatcgg agatgttcta gcagttatgc agaaactggc
    22981 gcgtgatggg atgaacatga tcatcgttac ccacgaaatg ggctttgctc gagaggttgc
    23041 ggaccgcatt atctttatgg ccgacggaga agttttagta gatacgacag atgtcgataa
    23101 cttttttgac aatccaagcg aacctcgtgc ccaacaattc ctcagcaaaa ttatcaacca
    23161 cgaaagtgac aaagtcaaat aaggaggcgc ctatgaaaaa aaaattcttt ttatcagcat
    23221 tattgattag ccttttcggc cttgctgctg ccaaaccagt ccaggctgat acaagtatcg
    23281 cagacattca aaaaagaggc gaactggttg tcggtgtcaa acaagacgtt cccaattttg
    23341 gttacaaaga tcccaagact ggtacttatt ctggtatcga aaccgacttg gccaagatgg
    23401 tagctgatga actcaaggtc aagattcgct atgtgccggt tacagcacaa acccgcggcc
    23461 ccctcctaga caatgaacag ggcgatatgg atatcgcgac ctttaccatc acggacgaac
    23521 gcaaaaaact ctacaacttt accagtccct actacacaga cgcttctgga tttttggtca
    23581 ataaatctgc caaaatcaaa aagattgagg acctaaacgg caaaaccatc ggagtcgccc
    23641 aaggttctat cacccaacgc ctgattactg aactgggtaa aaagaaaggt ctgaagttta
    23701 aattcgtcga acttggttcc tacccagaat tgattacttc ccttcacgct catcgtatcg
    23761 atgccttttc cgttgaccgc tctattctat ctggctacac tagtaaacgg acagcactac
    23821 tagatgatag tttcaagcta tctgactacg gtattgttac caagaaatca aatacagagt
    23881 tcaacgacta tcttgataac ttggttacta aatggagcaa ggatggtagt ttgcagaaac
    23941 tttatgatcg ttacaagctc aaaccatcta gccatactgc agattaagga ggacacccca
    24001 tgacagattt atcatcttgg acagcctatt ttcaggattt tggacaattt ttcaatggtt
    24061 tcctcttcac ccttgcccta gcgattggat cctttatcct cgccatggtc ttaggaatct
    24121 tctttggtgc catgtcaacc agtaaacgtc caattttacg cattttagct cgcatctttg
    24181 tggaatttta ccaaaacact cccctcttgg tgcagtttgt tattgttttt tatggcctac
    24241 ctcttatcag tgaccatact atcatgattc cgatttattg gacagctgtt ctctgcgtgg
    24301 gactctatca cggcgcttat atcgctgagg ttattcgttc agggattcag tctattccta
    24361 gtggtcagat ggaggccgcc ttatcgcaag gttttaccta tatcagtgcc atgcgcttga
    24421 ttatcttgcc tcagaccttc cgcatcattc tccctccatt gaccaaccaa attgttaacc
    24481 tcatcaagaa cacctctact gtagctatca tctctggagt agacttgatg tttgtgacta
    24541 agtcttggtc ggctctcaac ggaaactata tcccagcctt tttaggtgct gctcttctct
    24601 actttgccct atgcttccct gttgcccagt ttggtcgcaa gatggagcaa gccaacaaaa
    24661 aagcctattc actttaggag gttactatgg aatctatttt agaagtttta accccagata
    24721 acctagtctt tatctttaaa ggatttggct tgaccctcta tatttctctg attgccatca
    24781 tcctctctac tatcatcggt acggtgctag ctgtcatgag aaatggcaaa aattctgtct
    24841 tgcgaatcat ctccagtatt tatatcgagt ttgtgcgcaa cgttcccaac cttctctgga
    24901 tttttactat ctttttggtg ttcaaaatga aatccacacc agcaggtatt acagccttta
    24961 ctctctttac atcagcagcc ttggctgaga ttattcgagg cggtctcaat gccgtagaca
    25021 agggacaata cgaagcagga atgtcacaag gcttcacctc agcccaaatc ctctactaca
    25081 tcattctccc acaagccatc cgcaaaatgc taccagccat catttctcag tttgttaccg
    25141 tgattaagga taccagtctc ctctactctg ttatcgccct acaagaactc tttggagcca
    25201 gccaaattct catgggccgt tatttcgaac cagagcaggt cttcagtctt tacatcctga
    25261 ttgccctcat ctacttcagc tttaacctag caatttctag cctgtctcat atgctagcca
    25321 aacgttggca acaagccgca gaataatact cttcgaaaat ctcttcaaac cacgtcagct
    25381 tcaccttgcc gtatatatgt tactgacttc gtcagttcta tccacaacct caaaacggtg
    25441 ttttgagcag cctgcgggct agcttcctag tttgctcttt gattttcatt gagtataaac
    25501 agccactctc caattcaatt ggagagtttt ttgatgagag tagatattta tagacctcaa
    25561 tttgacacca tacattttct atgataaaat gtaacaaatt catttacaac aaatcgaaat
    25621 cgaaatctaa atgaaatgga attttctatg aaatcagtca aaggactact ctttatcata
    25681 gctagtttta tcttgactct tttgacttgg atgaacactt ctccccaatt catgattcca
    25741 ggactagctt taacaagcct atctctgact tttatcctag ccactcgtct cccactacta
    25801 gaaagctggt ttcacggttt ggagaaggtc tacaccgtcc acaaattcac agcctttctc
    25861 tccatcatcc tactaatctt tcataacttt agtatgggcg gtttgtgggg ctctcgctta
    25921 gctgctcagt ttggcaatct tgccatctat atctttgcca gcatcatcct tgtcgcctat
    25981 ttaggcaaat acatccaata cgaagcttgg cgatggattc accgcttggt ttacctagcc
    26041 tatattttag gactctttca catctacatg ataatgggca atcgtctcct tacatttaat
    26101 cttctaagtt ttcttgttgg tagctatgcc cttttaggct tactagctgg tttttatatc
    26161 atttttctat atcaaaagat ttccttcccc tatctaggga aaattaccca tctcaaacgc
    26221 ttaaatcacg atactagaga aattcaaatc catcttagca gacctttcaa ctatcaatca
    26281 ggacaatttg cctttctaaa gattttccaa gaaggctttg aaagtgctcc gcatcccttt
    26341 tctatctcag gaggtcatgg tcaaactctt tactttactg ttaaaaattc aggcgaccat
    26401 accaataata tctatgataa tcttcaagcc ggcagcaaag taaccctaga cagagcttac
    26461 ggacacatga tcatagaaga aggacgagaa aatcaggttt ggattgctgg aggtattggg
    26521 atcaccccct tcatctctta catccgtgaa catcctattt tagataaaca ggttcacttc
    26581 tactatagct tccgtggaga tgaaaatgca gtctacctag atttactccg taactatgct
    26641 cagaaaaatc ctaattttga actccatcta atcgacagta cgaaagacgg ctatcttaat
    26701 tttgaacaaa aagaagtgcc cgaacatgca accgtctata tgtgtggtcc tatttctatg
    26761 atgaaggcac ttgccaaaca gattaagaaa caaaatccaa aaacagagct tatttacgaa
    26821 ggattcaagt tcaaataagt aatcatactc tcctgggatt tcccaggaga gttttttata
    26881 gccaaatgaa ataagaccgg aacgatggaa ttttggcatt caaatcaatt gataagaata
    26941 ttttatagtg gagtgaaata ggatacgaac aaattgatta ggaaaatcaa atgaatttat
    27001 agaaatcttt tagcagttat gttgtcctat tctagtttca aaacgctata gaagcagcat
    27061 tgtactagtc tagattcagt ttactatact aaaacgagta gcttgaaatc aaaaaaccca
    27121 ccctcacagg caggttttat ctgtattatt cagctagatt atgctttacc ttctgaaccg
    27181 aatacgtcga tacgttcttc aaccgatgct tggatagctt ttacaccgtc agccaagaat
    27241 ttacgtgggt cgaagagttt tttcttgtcg tattctgctt cgtttgcttc gtagtcacga
    27301 gcaaatttac gagttgcgtt agcgaatgcg atttggcatt ctgtgttaac gttaactttg
    27361 gcaacaccaa gtttgatagc tgcttggatt tgctcatcag gaatacctga tccaccgtgc
    27421 aatacgattg ggaatcctgg aagagcttct gtcaattttt gcaagtggtc aaggtcaaga
    27481 ccttcccagt ttactgggta agggccgtgg atgttaccga taccagctgc caagaagtcg
    27541 ataccagttt caaccattgc tttagcgtct tcgattggag ccaattcacc tttaccgatg
    27601 attccgtctt cttcaccacc gatagtacca acttcagctt ctactgagat acctttagcg
    27661 tgtgcttttt caacaacttc tttagccaat ttaaggtttt cttcaactgg aaggtgtgaa
    27721 ccgtcaaaca tgattgaagt ataaccaact tcgatacact caagtgcatc ttcgtagtga
    27781 ccgtggtcaa ggtggatagc tactggtaca gtgataccca ttgattcaac aaggttagcg
    27841 atcaagttgc gagcaacttt gtaaccaccc atgtatttag cagcacccat tgaagtttgg
    27901 atcaaaactg gagctttttt agcttctgct gcgcgcaaga tagcttgagt ccactcaagg
    27961 ttgtttgtgt taaatccacc aactgcataa ccgttgtcac gggctgcttg gacaaatttt
    28021 tctgctgaaa cgattgccat tttatcaggc ctcctgtata tttttatggg tcatcccatt
    28081 tacattgttc attttatcac tttttgccaa aaaaatctag tttttcccgc agtttcgatt
    28141 gattttcttc taactccatc tatgtaaacc ctttctctcc ctagtcttgg acgacttttg
    28201 gaaaatctat aaagaaggtt aaacgattct cctccatctc gaaacgataa gctaattttt
    28261 catgttctaa tagactctta accacaaaga gccccatacc agaccccttg accttgcgac
    28321 tagcattgtc agaaaaagac tgggctagtt tttcttgttc ctctgagcta cagctatttt
    28381 cgataaaaag ttctccttct ctttctccaa ttcgaactaa gccacctgga acagagtgct
    28441 taatggcatt gctgatgaga ttagaaagaa tcaacttcat aactgatggg tttagataag
    28501 cctgctgatg ggtcaaacta ttgtctatct ggagctctct ttccttggct agcaaggcat
    28561 aatctttgac cagattttgc gtcatctgga ggaggtcaat tgtttcccta tcatctcgca
    28621 attcctgcac agaagagagg gaaagtatct gcagaacatg gtgattgagt tcatccacaa
    28681 tccccaaggc aactcccaga tactggtctc tatccttata acgaccgata ttctctctca
    28741 tattttcgat taggattttc aaactagcca gcggtgtttt caattcatga gaagctcctc
    28801 gtaggaattc gaccttcatc ttctccagct ggagaatggc ttcattcttt tcatgcaagt
    28861 ccgcaataac agtcaagaga tgctggtaga ggctattgat ttgttccttg agattaccta
    28921 tctcatcctt agaatccacg cgcaatcgca cttgggaatc caggtccatc atccgacggg
    28981 tcacccgctt gatttccaaa atcggtgcaa caatagtccg agcgtagatg taggccacca
    29041 agagggaaat cagaaaggag gccagcaagg tatagggaag aaactggaga ctgatttgct
    29101 ccgcttcctt ttgtaaatcc atggaagcta gaaactggag aatcatagta ccaccgtctt
    29161 gcgttttcac ctcgcgctcc tcaataaaga gagaggttgt ctggcggtct gtgtccagag
    29221 gaagactgtc cttgacttct aacttgtcct cggtcatctc acctttgacg gtccccttga
    29281 tatcactagt ctgggaatac aagtctaaca cttgctcgat actctgccta tctttccctt
    29341 ctagggactg ggcaatggct gttgcctttt gaccaatggt ttcctgacga tgactcagat
    29401 aagtcgaagg aaaaagaaaa taaatagcta aatgaaggca gataaccaga acactaaata
    29461 tcgagaaggt atagataaat atctttgcaa ataaacctgt tcgtttcatt ttcgctccaa
    29521 tttataacca acattgcgca cagtgaggat acaatccaag tctagctttt tccgcaattc
    29581 cttgatataa acatcaataa cacggtcaaa gggaacctca tctgtcgctt tccagacggc
    29641 atcgataatc tgagatcgag tcaaggcccg gccttcattt ttcactagat agtccagaat
    29701 ttccaactct ttggcattga taggcacttc ttgacctgcg atgcttgcac tgtagctttc
    29761 aaagtccacc ttggtatcct tgtaagaaaa gattcgtcct gtatcgtagt agcgcttgaa
    29821 aatcgcgtcc accctcactt ttaaaaggga gagggagaaa ggtttttcca gatagccatc
    29881 tgccaaagag gcaaaggcac tcatcttgta ttcctcatcc tgaaaagctg tcaacatcaa
    29941 gacaggaacc tgactggttt tacgaatctc agctaggact tctaagccgt tgagcttggg
    30001 catctggata tccagtaaaa ccagggccac ctcatagcta gaaaattgct ccagagcttc
    30061 ctgaccgtcc gctgcctcaa tagtttcata gccacaatcc gtcaaataat cactgacccc
    30121 ctcacggatc atctcttcat cttctacaat taaaattttc atactttaac tgctctctat
    30181 tttttatttt tcttagaata aatacctact ctattttcta ttatagtctc ttgctggcct
    30241 tttgtctgta agtaactgac cactagataa aacgttgtga aattcctttc tcataaattc
    30301 cataacttta gtatattata tttaagcact aaagtacaaa gaaagcaact gaaagcaatg
    30361 attttcacca ctgctttcag atttattttg aattgttaaa tagccattcc tatccactat
    30421 tcttgaatag aaacacaaga tgcaatcttt attccagact cattttttca aatttattca
    30481 ccatccagca agagctcttt tggttgtttt ctaaggagat tgcttgaagc aagcgccata
    30541 acgagaacca ctagaaccaa ggcaaggaca aaaatgatga taaagtctga tgtctgaata
    30601 gaaatgtcta ggctcgacaa ggtcttgcta aagccatcta cttctgcacc accaccaagg
    30661 ttagaggctt gagccgcctt actagcctgt ttggcaacac ctgaagtcac attggcaagg
    30721 acagtgtttc caattgcacg ggcagtgtaa ttagctagga agtaagcaga aactagagca
    30781 gggatagcaa tcaagataga ttcggtgatg aattgaccca agatacttgc ctgcttgagg
    30841 ccgatagaga ggagaattcc cacttccttg cgacgggcgt tgatccaaag gctgagcaag
    30901 agggcaagga ggagaactga gaagctcaag ctaccccaga agaggaggtt ggccatcttg
    30961 tacataccag agatagattg ctcaagagct gggtagttag aggagctctt gacgagtgtg
    31021 tagctcttcc agttgatacc actgatgcca ttcaactctt tcataacatc atccaagttc
    31081 ttgtctgctg ttacaaagaa ggttgcgtcc ccataaatgg ctgtgtcttc tgtgtatcca
    31141 taaagttttg cagcagtgtg aatgtctgta atagctgtgt tttcgtaaag ttcttgtgag
    31201 taggttactg ctgacttatt atgaccatca aagagtccct tgattgtcac ttcaactgtt
    31261 tccttggctc ctttttcatt atctgcatcg tagatattag agtccagttt aaccttgtcc
    31321 cctactttcc agccgtgttt ggctgccaag tccttgtgca agaggatttt atccttgtcg
    31381 tcgttggtta agtgctctcc ttcgactagt ttataagaac cagagacaaa cttgtcttct
    31441 ttagaggagt cattgacacc tgtaatcatc aagctacttc caaaacgttt ggcacgatca
    31501 gcagtgagat tcttcttggt ttctggcgtt tcaatcaggt catatccagt caaatctccg
    31561 atagcgttga tacgtttgac ataagactca atggccttgt tttcggtgat ttttttgatg
    31621 tcttcaccct tgatattccc agcaccgcga ggcgttcctt ggttgacgcg acgattgatt
    31681 tgcatggaga agctattggt gatattttta aaggtctcct gagaagcctt ggcagtagct
    31741 cccttgattg acaagccgac caaactcaag ctcgccatga ggagaataat caggaagatg
    31801 acaatcgatt tgaaaaactt ccttgtaaca taggcaaatg cgttgtgtaa catagattcc
    31861 ctttctagat tttgttttaa tcattctatt aaagtaagct caaattattt actagtattg
    31921 cgcgtttcag tcagtttctt atcctttaat tcaagtgtaa tatctgacgc ttgtgccact
    31981 tctttactgt gagttacgac aatcacacat ttacctgttt tctgggcaag tgatttgagt
    32041 agttcgacaa tatctccagc agttttagga tccagatttc ctgttggctc atcagctaga
    32101 ataactggag cttctgagac caaactgcga gcaatggcaa cacgttgctg ttgaccacct
    32161 gataactgga gaacattccg cttgatctgg ctttcatcca aaccaagctc aagaagtgta
    32221 ttcttgcttg cctttttgtt gaccaatcgg atattttcca gcggagaaag ataatctatc
    32281 aagttataat tttgaaagac cagggaaata tggtgcatgc gatggtaaga atagcccttc
    32341 ttacgaatat cctctccttg aaaaaggata gaaccttcaa caggactatc tagaccagca
    32401 agtagggaca agagtgtgga ttttcctgct cctgactccc caataatact gtaaaatttt
    32461 ccgggttcaa aattataatt gatctgatat aggactgctt cagcagtatt cttataacgg
    32521 taggtaacat cttgtaattg taataaagtc atgatttctc cttcttaact aatagatgat
    32581 aaaatttctt tcggtgattt tctaaataag aataggaaac aaagggctac agataagcaa
    32641 ctaagcagaa ctagaaaaac ataggattct gcaaaagata agatgctagt tgataaactg
    32701 cttgctttgg ctagtgtatc ttgtaagctt gcctgatctc cacttgctag tagagtttgg
    32761 agtaggtaag ttgtgattcc gtttcctgca acaaatgctg gaagcaaagc tccaagagat
    32821 accaaaacta cctctaaaca gaattgtagg aaaatcgagc tcttgccttt tccaagtgca
    32881 agtaaaatcc ccacttcata gacccgttct ctcaaccaga gagacaaaac cagaattaag
    32941 gctccagctc ctgctatcaa catcccataa aggaagatgg tcaggaaggt ttggaaagtt
    33001 gcaactgagt ctttgatttg ttcaaaagcc ttgttttcct tttcgacttg gtagccttga
    33061 ttttccaagg ccaagttttc tacctgcttc atgagtccgt ccatttcctt aggattttct
    33121 acatagaagc gtgctgcact gacttgagct tcactattgc ccaaaagggt ttggctactt
    33181 tcatagtctg taaagacttg attttcactg aagtcagaag acaagcctgt gaatttctct
    33241 tgttttttac cagaaaagat gccgataatc tcaaactcta ctgtttgtcc ttttccagat
    33301 tcagactgac cagcatccaa gccaatcttg tcatgaagtg aaagaccgtt cttcttagcc
    33361 aattcttcgt ggataaggat tttcttggaa ttcccttttt gaaggtgtcg cccttctttt
    33421 agattgaaag ccgaactggt aaaggttaca tccttggatg aatcctcaag agccgttaag
    33481 ctaaccaagt tattgtctgc agctgataaa tcatcacgct ccacgctctg ctcgccagtc
    33541 actgcttcct tgtcttttag ttttgcgacc gtctcaagtt caggagagac attttccagc
    33601 cccttaatct tgcttacaga tgctaggtct gacaacttga atgtctgacc attctctatc
    33661 ttcttaatag aaaaagatgt attgagtgat ttataaagat tgctttctac tgttttgttg
    33721 gacttcatca gagtcaaaca ggctgaaatt ccggccaata agaccaataa aatcagaaat
    33781 aaaataaaac ttctcagtcg ctttctgctg acataagccc aagatctttg gattggattc
    33841 atttgtcacc tccatatttg taagactatt ataaaaccca aatatgaaat gtttatgaaa
    33901 tacgaaaaaa aatatcgagt aggggataat ctctagcccc tctcacacca ccgtacgtgc
    33961 cgttcggcat acggcggttc aactaacttt taacgcatgt cgttcaaggt aataatccaa
    34021 acacgaaacc agtccacgtt tttcaagggc tggttttgat atagcacgtt taagtaccga
    34081 cttctgagct actaattgat agtggtcgcc ccagccagat accttatctg ctatccattt
    34141 aggaacccct aacttaagca atccccataa tcgtctcgat ttcttcttcc attgcttcca
    34201 gataatcact cgtaggcgag tacgcaagcg ctcatctatg ctagtgacta tacttttcat
    34261 atttataatt cattcctttc gtttcactca aggcacaaca cagaatgaaa aagtgttgtg
    34321 atctttattt tgttttataa taatagtgag aaagcctatc actactacaa atcacgggga
    34381 ggtgaataag tgagtggtac agccactacc tcgcatattt tgtcacatca tttaacggta
    34441 cataataagt tgtaccatct gaataagttg ctacaatatc atttgcatgc tctccttcac
    34501 ctttagcaaa ggttggagct cctgctggat gatttttatt tgcctctttc aatttttcaa
    34561 taatggcatt tttttctgta tcttttatat tatcaggatt tttcactaag attttgtctg
    34621 gatatgtcgg tttagcagaa acaattttta ctgttatttc ttttttattc gaagcacttg
    34681 tccagtttcc agcattatct ttagtattta attttacagt aattcctgaa ctaggaactt
    34741 cagtagcagg ttgattatca acattattca actttaattt caaaagagct gttgcatcag
    34801 acgttttatc aatcgttata tataatgata aattgttatt ataaccagtt tcttcatatt
    34861 tagctgtttg tgagctactt gaaacagaac tgaaattata cccactacct ccctgattat
    34921 cttcaatgct tacatctaaa tgaacttccc cactattatt tggattagca acaactgtta
    34981 tagtaaaata acataaaatt cacataaata gattagggaa atcaaagcaa cttctaggaa
    35041 tgttttatac tcttcgaaaa tctcttcaaa ccacgtcagc ttcaccttga cgtactcaag
    35101 tacagcctgc ggctagcttc ctagtttgta ctttgatttt cattgagtat tagcagtcac
    35161 agtgtacttt cccagcatca agccactata actctgcaca taaaaatgga gaagatggca
    35221 atcctcttct ccaaatatta acttctttac aaaccaacta tagttgacaa agaacctaaa
    35281 atcaattgat aacacaaggt caggtcggtc aactctttca actgaagccc tgtcaactct
    35341 tcccatttat caatcttgta ttggagagaa ttgcggtgca gatagagttg ctgggctgtt
    35401 ttagtgagaa cagcactatt ttcccaaaga gagagaatga tttcctgaat ctgatcttga
    35461 tccaaaatca tctggtgtag acattcttta attggcttca agtccacgag tctttctccc
    35521 atactccaaa gatagagctg agaaaaagta tgaacacctt ggtgaccctg acgccaccat
    35581 gtcttgaaca aatcccgctc agctttgatt aagtctgata gggcttgatg tcctgtctga
    35641 gaccaaacct gacccaacat gatagaaaga cgaagtccaa agtcatactc aactgcttca
    35701 atcatatcac ttaaaatatc tcttacagaa gtgtatttgt cttgttgaag cacgaaaaca
    35761 taatcctgag ctccgacctg taacactgtc tgacaattcg gaaaaagagt ccgcatcata
    35821 tctagccaag aagccagatt ttcctgctga aaataagaaa gatggcaata aaccaactga
    35881 atctttttaa aaacttgcgg tgcctgtccc ttgccctcaa ccagatagga ataccaaggg
    35941 tttagcgaac gagcctgctc ctgctgggtc aaaaggacaa ccaactgctt ttcacgctcg
    36001 ctgagcccag cttcctccag caaaatccac tgctgagaag ctaaagggag cgtgagatag
    36061 ccctctttct ctactggttg gtctgaaatc cgagcctcag gaaaccagtc ttgtagttct
    36121 tttgccctca tgttctagcc ctccactttt tggatgcacc atgaaaccaa actctcaaga
    36181 cgttccagat tctcagtcat atggagatag cccataaccg cttcaaatcc cgtggacata
    36241 cgataagtca cgacatctgc atttttagcc tttgtgtggc tattggtatt gcggccacgt
    36301 ttgtagattt cttcttcttt ttccgttagg acctgctcct ccaacatgag agcaatcagg
    36361 cgagcctgag ccttggctga cacatacttg gttgcttctt gatggagttt attgggtttg
    36421 gtcatacctt tgaggatgag gtgacggcga atatacatag aatacaccgc atccccctca
    36481 aaggctagcg caatcccgtt aatgagattg acatcaatca cgtgtccacc tcactccatc
    36541 cttggtatca aggagcttaa ttccttgagt aaccaattgg tcacggattt ggtctgctgt
    36601 cgcaaagtca cgattggcac gcgcctcttg gcgtttttga atcaagtctt caatctctgc
    36661 atccaaaact tcctcaacaa agacaattcc aaaaatttct aacatatctg caagagcttg
    36721 cttgacactt gcatcatagt tccctgaatt gatccatttg gccatttcaa agacaactgt
    36781 gataccgttg gcagcattaa aatcttcatc catagctgct acaaacttat ctttaaagtt
    36841 ttgtaactct tgggcatcca cgtttcctgt aaatggttgt tcgtaagtat tcttcagata
    36901 cttgagattg gtctcggcat cgcgaactgc cttttccgtg aagttgatag gcttacggta
    36961 gtgctgggtc gcaaagaaga aacgaagtac ttgcccatca agagttttaa gggcatcgtg
    37021 taccgtaatg aagttaccca aggacttaga cattttgaca ttgtcgatat tgacaaagcc
    37081 attgtgcatc cagtagttag caaaagcctt gcctgtttta gcttcagatt gggcaatttc
    37141 attggtgtgg tgtggaaact ctaggtcagc tccaccaccg tggatatcaa tggtatcacc
    37201 taaaatctct gtcgacatga ctgaacactc aatatgccaa cccggacgtc caggtcccca
    37261 aggactatcc caagaaatct cacctggttt ggaagatttc catagagcaa aatctacagg
    37321 attttcctta cgagccgttt cttcatcggt acgacctgaa gcacctagat ccaaatcttc
    37381 caaggtttta ttagccaatt tagcatagtt gtgggatttt tctacacgga aatagacatc
    37441 cccttgactc tcataggcaa agcctttctc gatcaagtct tccacaaaac ggatgatgtc
    37501 tgccataaac tccactacac gcggatggcg agtcgcaggt ttcacgccta aggccgtcac
    37561 atcctcacga aaggcagcga tgtacttatc cgcaacctcc tgaggcgtga taccttcttc
    37621 cctggcacgg ttgataatct tatcatccac atctgtaaaa ttggaaatat aggcaacctt
    37681 atacccacgg tactcaaaat agcgacgaat cgtatcaaaa gctaccgtcg aacgggcgtt
    37741 tcccacgtgg atatagttgt acaccgttgg cccacaaaca tacatcttga tcttgccgtc
    37801 ctcaatcggg acaaattctc gcaaatcacg agacatggtg tcatagattt taatcataaa
    37861 tcataatcaa gaaagctgaa atccaagaac aattagtttc atcactaaaa gttcaagtaa
    37921 atttcagtcc gaatatctct acacttcgga atcccttgct cctttctcat tcagataaac
    37981 cacctgagtc tgtttgacaa agccaatttt ttcatacaaa cgtttggcac ctacattgct
    38041 atcttccact gcaatctgaa attccttgtc attttgctca attagttggt tgacgaggga
    38101 ttttgctaag tagcttccat agcctttccc acgttcaggt tccgatattg ctaaaccgta
    38161 gaagtaattc gtattagtcg ataaatctac cgtgcaagtc ccaataacct gaccgtcttt
    38221 taacaaaata tataagcgac tttctggatc cttcagagct tcagcgacat atctatccac
    38281 aacttctctc gattcatgtt cctctgaaaa tgcctgaaat tttaattgac taatttgatc
    38341 ctgatacgaa ctatctgcta acaaaacttc aagatgggaa acatttgcta acggataagg
    38401 tcttctatcc ttacctaacc aagtttctgt ctcttcatcc tcgattagtc cccagttact
    38461 ggcaaagtca ggatgattct ctaaaaaaat acgttctgtc tgaaaagtga ctgaccgaat
    38521 ggggaaagaa gctgtttctc tctcaaaact agtaaacaat gcacgcgcaa tcccctgacg
    38581 gcgatgacct ggatgaacca gtatcgttac ttctacatct tggtcatctg cataaacagt
    38641 taataaacca acaagttcgc ctttttcata ataaaggaaa aaggcgggca tgtttgggtc
    38701 aaaattaagc atgttagaga gatagggatc gcgataggta ccgtcatagt tttggcaaca
    38761 gttaattact tttttcgcct cagatagctc ctcttggctt aacttgtttc ttgcttgaat
    38821 catataggta tcctctacaa accagacgat ctgtgactgg catctttagc ctgctcgagt
    38881 ttattgacat aatactctcg tttttcttcg acttcgtgaa tgacaggctc atctttctta
    38941 ccatgaagac ggacaatctt ggccggaata ccgacaaccg tcacgtcact aggtacatct
    39001 gctacgacaa ctgctgcagc accgaccttg gcattttcac caatttccac aggcccgata
    39061 acttgggcat gggctgatat gagggctccc tttcgtacag tcggatggcg tttgccacag
    39121 tctttccctg ttcccccgag agtcactccg tgatagagaa gaacgccttt ttcaacaatc
    39181 gctgtctctc caatcaccag accagaacca tggtcaataa aaacacctga atcaatctgg
    39241 gctcctggat gaatctcaat ctgagtccaa aagcgccaaa actgactgta catacgagct
    39301 aatagtttga agccgtgctt ccagagaaaa tgcgagagac ggtgggccgc caaggccttg
    39361 acacctggat aagtcagcaa aacctccaaa gtggtgcggg ccgctggatc attttctttt
    39421 acaatatcaa tggtttcgcg ccaccacccc atacatttct ccttttctta ttctgaatct
    39481 tttggtgttt ctgtaaattc tttcttaggt ttgtgatcct tttgatgacg tgggcggtga
    39541 gggcgctcag acttttcacc tttttcatca tgctcaggtt ttggcggacg aggtagaaga
    39601 gccttcatag aggcatcgat acggcctttt tcatcaattt tgataacctt aacatcaact
    39661 tcatccccga tttctaccaa atcctctaca cgattggtac gagtccaagc catctcagag
    39721 atatgaacaa gggcatctgt cttatcaaag aggttaacaa aggcaccaaa tttctcgata
    39781 cgaacgactt tagcacggta aacttcatcc actttggctt cacgaaccaa accagcaata
    39841 atttctttgg cacggttaat agcatcttgg tcactagagt agatagacac atttccttct
    39901 tcgtctatat caatcttaac acctgtttca gcgataatct tgtcgatggt ttctccaccc
    39961 ttaccgatga caatcttaat cttgtccaca tcaatcttga tcgtatcaat tttcggagca
    40021 gttggagcca attctggacg aacttctgga atggttgctt caatgacatc aaggatttca
    40081 aaacgcgctt tcttggcttg agcaagagcc tccgtcaaga tttctgcagt aatcccttga
    40141 atcttgatat ccatttgaag ggctgtaatc ccatcacgag tacctgcaac cttgaagtcc
    40201 atatctccaa agtgatcttc caaaccttgg atatctgtca atactgtgta gttatttcca
    40261 tctgagataa gtcccatagc aataccagct actggcgcct tgattggcac accaccagcc
    40321 ataagggcaa gagttcccgc acagatagaa gcttgagatg aagaaccgtt tgattccaaa
    40381 acttctgcta ctagacggat agcgtatggg aattcttcca agcttggcaa gacttgagca
    40441 agagcacgct caccaagggc accgtgaccg atttcacgac gacctggcgc accgtaacga
    40501 cctgtttccc ctacagaata ttgagggaag ttatagtggt gcataaagcg tttcttgtac
    40561 tccagatcca aaccatcaat gatttgagtt tctcccatcg gagccaaggt caagacagaa
    40621 agggcttgcg tttgtccacg agtgaagaga cctgaaccgt gcacacgagg aaggaagtcg
    40681 acaaccgcat ccaaaggacg gatttcatcg accttacgac catcaggacg caccttgtct
    40741 tctgtaatta aacgtcgcac ttctgcgtgt tccatttgtt ccaagatttc agccacatca
    40801 cgcataatac ggtcaaattc ttcgtggtcc gcatattttt cttcgtaaac ggcagtcact
    40861 tggtctttca ctacttgagt cgcagcttca cgggccaatt tctcttctac ttgaactgcc
    40921 ttttggaggt cactgttgta ggctgcaatg atttcagctt gcaattcagc atccacgtga
    40981 agcaattcca cttctgcttt ttctttaccg acagcagcaa cgatttcttc ttggaaggca
    41041 atcaattctt tgacagcttc gtgcccttta aggagcgctt ccaacatgat ttcttctgac
    41101 aattctttag caccagactc taccatgttg atagcgtgct tggttccagc tactgtcaat
    41161 tcaagaagag attgctctgc ttgttcttga cttgggttga tgatgatttg gccatctaca
    41221 tatcccactt gtaccccagc aattggtccg tcaaatggaa tatctgaaat agacagtgcc
    41281 aaagatgaac caaacatagc agccattggt gcagacgcat tttcatcata agaaagcact
    41341 gtattgatga cttggacttc attacggaaa ccttccgcaa acataggacg aatcggacgg
    41401 tcaatcaaac gcgctgtcaa ggtcgcatct gttgaaggac gtccttcacg tttcataaag
    41461 ccaccaggaa acttcccagc cgcatacatt ttttcttcgt agttgacttg gagtgggaag
    41521 aaatccccag ttgccatttt cttagacata acggcagcag tcaagacagt tgactcaccg
    41581 taacgtacga caacagatcc atttgcttgc ttagcaacct gaccagtctc tacaattaac
    41641 tcacgacccg caacagtcgt ttgaaacact tgttttgcca ttttaatccc ctttggattg
    41701 atgaaattat acgccttgcc tacaaagatc aagataccaa ggacgtcaaa aacaaagtaa
    41761 aaataggaaa ctgacgaagt cttcgatgaa gacaagacag tttatctttt ttacacagct
    41821 tttcggccgt gttcaattac acaagatatt ttggacggtt cggcttgccg aacatttctg
    41881 tagaaaaata ggaaggtgac gtcgcactcg acgagtgcta ggaagcttat cttttttcct
    41941 aagaaatgag accaaaattc aattactcaa gatactaagc cgtcaagcaa ctcaaaggaa
    42001 aataggaaat cgaacgacgg agcgactgct cctagggaga tttatctttt tccacagagt
    42061 tgtaggcgag ttcagttttc aagatataaa tcattagaaa ggtttaatac taaagtatct
    42121 aaagctttca cgctaatcgc tatcgggcga ttagctaaat gctttactaa ctctctcgtc
    42181 aaataacatc gatttgactc actcgtgtcg ttaaatctta cagtttaaat gcattgtatt
    42241 atttaatacc ttcatctttg tatcaagtac gtacagaatc tattttatca tatttttctt
    42301 aaaaagtgag gtctttacca ttaaaaagga accattcccc tcacctgaga agaatggttt
    42361 gcttttatta tcctagagac tggtgattaa acaaggcatg ggttgcttga tggatgtatt
    42421 ttgctgtatc agcattattc atcgtataga gatgcacacc ggcaacatcc tgagttacca
    42481 agtccacgat ttggtccact gcataggcaa gccctgctgc tctgagcgac tcagggtcat
    42541 gctcatactt gtctaagatg gctttaaatt tgcgtggaag atggatattc tcacaagtct
    42601 tcaagagtcg gagagcctga tttcgattca gaattggcat aattcctgca tgaatgggaa
    42661 catcaatccc agccaagatg cacttgtcct gaaaatcata gaagcgctca ttgtcaaaga
    42721 agagctgagt tacgaggctc gaacagcctg catccacttt cttcttaaga ttttgaatat
    42781 ctgaaatctg atttggcgaa tctggatgcc cttctggata gcaagctcca ataatatcaa
    42841 agtgaggggt ttgttccttg ataaactcaa tcaagtcggt tgcatagcgg aaatcctttt
    42901 gtggttccac gtctggaata atatccccac gaagagccaa gattttctgc accccaactt
    42961 tgtccaagtc agcaatagtt tcagcaacct tgtccttagt tagataaata gctggcaagt
    43021 gggcaatggt cggaatcgcc aaatcatttt ggataaagtc agccaaacga accgtcgttt
    43081 ccttgatatt aaatttatta ttgctggcag ttacactgat aaaatgggga gccaactcct
    43141 gcatatcctg caaggctgaa ataatgttat cattacccac ggctgggttt ggagggaaca
    43201 cttcaaatga gagtgacggt gtttggcgtg acatatgtaa taaccttttc tagttgattt
    43261 ctttttgaac aaccactgta tggagagaaa tccaatctta caatttctca cgcgcagctt
    43321 tagctgcttc aacaaggcgg atcaagcttt cttttgtttc tgggatacca cgtgttttca
    43381 aaccacagtc agggttgatc caaactttct tgcttggcac tttagcaagg atggcttcga
    43441 ttgtgttgtc gatttcgcct tcatttggta cacgaggtga gtggatatcg taaaccccag
    43501 gtcccacttc tgtttggaag tttttcgctt tgagttcgtc caagatttca aggtttgaac
    43561 ggctagcttc aaaggaaata acgtctgcat ccatgttatc gatagctggg atgatatctg
    43621 taaattctga gtaacacatg tgagtgtgga tttgtgtgtc tggcgctact gttgagtgta
    43681 ccaagcagaa ggcaggaata gcccagtcaa ggtagtcttc gtaccagtcg ctacggcgga
    43741 gtggcaattt ttcacgaaga gcagcctcgt cgatttggat gattttcaca ccagcagctt
    43801 caaggtcaag tacttcatcc ttgatagcaa gggcgatttg gagagttgaa tccttgatag
    43861 agatgtcttc acgtgggaat gaccagttaa ggatggtaac aggtccagtc aacatacctt
    43921 taacaggttt gtttgtacga ctttgtgcat agctagacca tttaacagtg atagggttaa
    43981 gacgagtgac atcaccccag atgattggtg gttttacccc acgcataccg tatgattgta
    44041 cccatccatt tttagagaag aggtatcctg acaagttttg accgaagtac tcaaccatgt
    44101 cattacgctc aaattcaccg tgaacaagga catcaaagtc aatatcttct tgccacttga
    44161 tccattcgtc aatcgtttca gcaaggaaag catcgtactc tttttgagac aattcacctt
    44221 tacggtaagc caaacgtttg gcacgaactt cttttgtttg agggaatgaa ccaatcgttg
    44281 ttgttggaag agctggaagt ttgaaagctt cttcttggat agcttcacgt tctgcaaagg
    44341 ctggcaaacg agtgtagtct gcgtctgtca agccagcgat acgcgcacga agttcagcat
    44401 tttcaccaac acgctcagtc gcaaaaagtt ctttgttggc tgcaagagct tctgaacctt
    44461 gaccattgcg gatagcatcc aagtcacgga tttcatccaa tttttcaact gcaaaggcaa
    44521 agtggttcaa gagtgctggt tcaaattctt cattagcagt tgtaaatggc acatgaagaa
    44581 gtgagcaaga gcttgtcaaa acgatgtttt cagctggaat ttgctcaaga acagccaagc
    44641 tcttttcgta gttgttgcgc cagatgtttt taccattgac aatacctaca tagagagtct
    44701 tgtcagctgg gaagccacct ttaacgagtt caagagtttt cttaccttca acaaagtcaa
    44761 gaccgatagc atctactggt aagtttacaa ggtcagcgta tacgtcacga acatcaccga
    44821 aataagtttg aagcaagact tcaagacctt ttttgtcagc caagagtttg ttgtaaaggt
    44881 tcaagaagag agctttttct tcagctgtca agtcttttac aagagccgct tcatccaatt
    44941 ggatgcgagt cgcaccaagt tcagccaatt tagcaaaaac ttcttggtaa gcagccacta
    45001 agctatctac gaagtcgtct gctttcacgc cttcttcaaa gtctgacaat tgaaggaaag
    45061 tgaagggacc tacaagaaca ggacgagtgt tcaatccaag ttctttggct tcttggaact
    45121 catcgaaaat cttgtgacca gccaatttta cttgagtgtc tttttcaaat ttaggaacga
    45181 tgtagtggta gttagtgttg aaccatttct tcattggaag ggcgcgaacg tccccttttt
    45241 ctccctggta accacgtccc aaagcgaagt agcgctcaag gtcagacaag tccaagtttt
    45301 gaacggatgc aggcaccacg ttgaaaagga aagccgcatc taggaagtta tcatagtgag
    45361 aaaagtcatt tgatggaatt tcagtgatgc ctttttcttt gacaatgttc cagtgtttag
    45421 cacgcaagtc ttttgctgct gctaaaagtt cttcttctga gatttctttt ctaaagtatt
    45481 tttcagttgt aaattttaat tcacggaatt cgcccaaacg agggaaaccg atgattgtag
    45541 ttgacatgat gtgtcctcca aaatttgttg ttgaaactat cttaacagaa aagaaagcgt
    45601 ctgtataatt gtaaaaaatt agggtttgat atagtttgaa actatatatc tgtttcggac
    45661 aaaagaaaaa gacttgaagc aaacgtctca aatcctttgt aattcttact ttacagctat
    45721 attccaatta gaatactaaa acatgttatt agtaattctt ataagtgact atgacctgtt
    45781 attagaaaag actataactg attctagtca actttttccc tgttcaagtg ggacgattgc
    45841 tagtgtcttt cctaaactgg ctaggacttt taagactgta tccaactgag gactagtctt
    45901 tcctgtctcc atcctagcta tgacaggctg gcttattcca ctgacttctt ccagcttttt
    45961 ctgactgatt ccttgttcat acctagcctc aatcaactcg ctcatgatag ccactcgcat
    46021 atcactttca aggatttcct ccttgctaaa gagctcagat ggacatcctt ccaattactc
    46081 ccaatagcac tattcttcat cacttaaccc tctttttttt acgtctatgt atttttaaaa
    46141 aaatgagcga attatgattc gatagattga ccagtgggtt taaagttggt gctagcctat
    46201 ttcttaagcg attttccttt tctaggataa agtagatcct gcttgcttaa ccccaatttt
    46261 ccacgatgaa tccaatagta aatggttgaa attcccacgt taaccccttt agccatcacc
    46321 atcatttcag gcgaaaattt ttggttatgt atagtggaga atcttttcct ttagtttctt
    46381 aagactgttg agcgtagtcg gcagaataaa tctctttgaa gcgccctttt ccaagacatt
    46441 gtcggactgt cccacgcttg atttcagtgt ggatagtttg aggagctttt ccaagtagag
    46501 aggcaatttc tctatttgat tttccttctt ttttccatcg ttcgattaag cgacggctat
    46561 cgattgtcaa atgtttgcct tttgtagtat aattgtcttg catttctgtg ccttttaatc
    46621 atttcaatct taaattggac tttttttact tgggttgtac ttaatctatg aggaagacaa
    46681 gaaaaagaat atcaatcaag taaagtcaca aagttacatt agctccgagc aaccattgca
    46741 aattgaggta ctcacacaat gattaaaaca tttctctctg ccctttcggt cattctcttt
    46801 tctatcccta tcataactta ttcttttttc ccatcttcta atcttaacat ttggctatct
    46861 acccaaccta tcttggcaca gatttatgcc ttccccttag ctactgcaac tatggctgct
    46921 attttaagtt tcttattttt tttcctatct ttttacaaga aaaataaaca aatacggttt
    46981 tactctggca ttttgctctt actatcgctc atattactat tattcggaac agataaaacc
    47041 ctttcttctg catcaaataa gactaaaacc ttaaaattag taacttggaa cgtcgctaat
    47101 caaatagaag cacaacatat tgagcgaatt tttagccatt ttgacgccga tatggctata
    47161 ttccctgaac tagctaccaa tatcagaggt gagcaagaaa accagagaat caaactattg
    47221 tttcatcaag ttggactttc tatggccaac tatgatattt tcacttctcc acctaccaat
    47281 agtggaatag ctcctgtgac tgtgattgtc aagaaaagtt atggtttcta tacagaagct
    47341 aaaacttttc atacaacacg gttcgggaca attgtattac attcgagaaa acaaaatata
    47401 ccagatatca ttgccttgca tactgctcct cctctgccag gtttaatgga aatctggaag
    47461 caagacttaa acatcattca taatcaattg gcttcaaaat atccaaaggc tattattgca
    47521 ggtgatttta atgcaactat gcgtcatgga gcacttgcaa aaataagctc tcatagggac
    47581 gcattgaatg cacttccacc ttttgaaaga ggaacttgga atagccaaag tccaaaactt
    47641 tttaatgcaa cgatagatca tatcttattg cctaaaaacc actactatgt tagagattta
    47701 gacattgtaa gttttcaaaa ctctgatcat agatgtattt ttacagaaat cacattttaa
    47761 ttattttata taaaattccc cctctagtgt tcataaacta gagggggaat ttgtatccta
    47821 ctatcgttta acgcacttct gcattcactt tttcttcgag cgatgcttgg attttttcca
    47881 tatagcgtgc gacttcttcg tccgttaagc tatcttctgg attttggaag gtcaagctat
    47941 aagccatgga cttcatacca agtcccaatt tctcacctga gaagacgtca aagagtttga
    48001 tatctgtcaa acgtttcacg ccggcagctt ggatagcatc tacaacttct tgatgagtca
    48061 cttctgcctt gaggagaagg gcaacgtcac ggctgactgc tgggaatttc gtgatttcca
    48121 caaatggaac agcaggttgg agcgcccctt cgatggctga aaggttaagc tcagctacat
    48181 acgtttctgg aatatcgtaa gccttggcag tgactggatg cacttggcca aggaaaccaa
    48241 gaacttggtc accgagtgaa atcacggctg tacgacctgg atgaaggcta gcgatttcag
    48301 atgttgctgt ataggttact tggagtccca aacgagtaaa tagggcttca aggattccct
    48361 tagcatagaa gaaatcaact ggaactgctg ctgtttggaa atctttttca gcaaccaagc
    48421 ctgtcaaggc aaaggcaaag ctgttgatct catttggaag ttcttctttt ggattacctg
    48481 tttgttcaaa gacttttcca atctcataaa gggccaagtt tttattctta cgagccacgt
    48541 tgtaggcaac ggtatcaagg atccctgaaa tcatattttg acggaggact gaacgatcca
    48601 cagtcattgg ccacatgagt tcagtaaggt tacttggttg agctgtgaac tcaactgctt
    48661 tttcaggagt tgtcagagca taggtgataa tttctgtcaa acctgctcct tcagcaatgg
    48721 tacgaacttg acggcggagt ttttgtgtca cagtcaattc accagctgta ccatcgtctt
    48781 ttggaaggct ggttggcaag cggtcatatc catagatacg agcgatttct tcaaagagat
    48841 cagcttcgat tgtgatatcc caacgacgac gtggtacgct gactgtaaag ctgtctgcat
    48901 ttccagaaag accaaagcca agacgacgga agacgtcttc gacatcagca taagacagct
    48961 cagttccgag gacacggtta acatcagcaa gagttgaaga aacttctaca tctgaagtat
    49021 caagctcacc cgctgaaacg atacccttac gcaccgtcgc acctgcaagt tccgcaatca
    49081 ggctagctgc cgcatcaagg gcttcattaa ctgttgccac attaattcct ttttcaaagc
    49141 gagaagatga ctcagaacga aggttcaggt gaccacttgt cttacggata gatttgccat
    49201 tgaaaacagc agcttcaagg acaacacgac tagatttttc agagatttct gttgcttgac
    49261 cacccatgac acctgcaagg gctactggct tgtctgcaac agtgatgact aggtcattca
    49321 cgtccaagtc acgttcttca ccgtccaagg tcaccaattt ttcaccagca cgcgcttcac
    49381 gcacacggat gtcagtccct tcaaatgtgt ccaagtcaaa agcatgcata ggttgaccaa
    49441 agtagagcag gatgtagttt gtcacgtcta caacgttatt gatgggacgg atgccttcgt
    49501 tcatgagaag gttttgcaac cattgtggac ttggtgcgat agtcacattg tccaagatac
    49561 gagctgcata gtaaggcgcc ttgtctgtcc caatgctgac agaaagggca tctgccgcag
    49621 cttcattagt ttctgttaga gtaaattctt taaagttgac tgccttgtca tagatggctg
    49681 ccacttcgtg agccactcca cacatagaaa gggcatctgc acggtttggt gtgatggaaa
    49741 gttcgatgat ttcatcatcc aagtctaggt aagaaaagac ttcctcacct ggcacggcat
    49801 tttctggcaa gatttggatg ccatctgcga attccttagg cacaactgag tcagaaattc
    49861 ccaattcacc aagtgaacag atcattccaa gtgactccaa accacggatt tttccttttt
    49921 tgattttgta gttatcagcg atacgagctc ctggaagagc caccatgacc ttgatcccag
    49981 cacgcacatt tggggcacca caaacgatct gacgctcttc ttcttcgcca acgttaacct
    50041 gacaaacatg gaggtgagtc tctggcacat cttcgcaaga caagacctca ccgacgacaa
    50101 tttttgagag accagcagct ggtgattcga caccctctac ctcgattcct gtagttgaca
    50161 ttttttcagc caactcttgt gatggcacat caatgtccac caattctttt aaccatttat
    50221 aagatacaag cataatttag ttctccagaa tgaccattgc cacactggtt cttttccttt
    50281 cctatcattt caatagaaga atcctcttct taccttaatt tctttctcag taaccaatcc
    50341 gtatctactt tttgaccaac cataaaatga tgttggctaa atttttcaaa accatatcgg
    50401 ttataaaagg cttgagcttt tgtattatgc tcccaaacac ctagccaagc ccaagaaaaa
    50461 ctattttttg tagcaagttc aagtgcgaat tcaaacagtt gcttacctag tccaaatcct
    50521 tggaattttt gtagcacata gaggcgttga atttcaaaag cgtcctctaa ttctctctca
    50581 gtttgagcac ttccccagtt gactttgaga aaaccagcta tctcctcctc atgcataatg
    50641 aaataggttt cagagtcagg atttcccaac tcagttgaca cagttttcag actataagcc
    50701 tcttcaaagt attcctgtaa ctgctcttcc gtattatcat acgcaaaggt ttcacgaaag
    50761 gtttgtttgg caattttagc caacacctca acatctgcca tttctacttt tctaatcatt
    50821 atttaaactg ttctgagaag cggacatctc cttggtagaa tccacggata tcgttgattc
    50881 cataacggag catagctaca cgctcttgtc caagaccaaa ggcaaagcca gagtatacag
    50941 tcgcatcgat accactcatt tcaaggacac gtgggtgaac cataccggcc cccataattt
    51001 cgatccaacc tgttttctta catacattac agccttctcc accacacttg aagcaagaaa
    51061 catccacctc aacagatggc tctgtgaatg ggaagtaaga tggacgcaaa cgaatttgac
    51121 gctcttcacc aaacattttt tggacaatca actgaagcgt tccttgaaga tcagccatag
    51181 agatattttt cccaactacc aagccttcga tttggttgaa ttggtgactg tgggtcgcat
    51241 cgtccgtatc gcgacggaag acacgccctg gcgagatcat cttcaaagga cctttagaaa
    51301 aatcatgggc atccatagca cgcgcctgaa ctggagacgt gtgggtacgg agcaagattt
    51361 cttcagtgat atagaaagta tcctgcatat cacgagctgg gtggtctttt ggaaggttca
    51421 tacgttcaaa gttatagtag tcttgctcca cttcaaaacc atccacgact tgataaccca
    51481 taccgatgaa gatatcttcg atttcttcac tggtttgtgt caaaacgtga cggtgaccag
    51541 tcgcaactgg acgacctgga agcgtcacat cgatactctc gctagccagt tgagccgcga
    51601 ctttcttttc ttccaagagc ttagctgttt cttcaaaagc agcagtcaag acatcacgag
    51661 cttcattgac gtgtttcccg atgattggac gcatctcagc agaaacatct ttcatccctt
    51721 tgaggatttc agtgagcgaa ccctttttac caaggacaga gacacgcaaa tcttgcatct
    51781 ctttttcatt tccagcagta atctgcttca agctagccag cgtttcttcg cgaagcgctt
    51841 ttaattgttc ttcaatagtt gacatatttc ctccatcagt ctctcgtaga taaaaagaaa
    51901 accacatgcc aaaaactcca ctcggagcgt tgacacgcgg taccatccgt tttcatctga
    51961 caagtcagac cttcatttct aaatccatgc gcaagtgaat tcacccagct ttcatataga
    52021 gagcttgcag tcacggctct cctccctgat atacttccct tgggctacta gtctttcaga
    52081 ttcctattca attactactt agtttatcag atttttacca ttcttgcaag acctatctta
    52141 cttctgcttg ttagcttatt cttatctaac tttatagcgg attgaaataa gatgaaaaca
    52201 aatcgattgg gaaagtaaaa ttaatttcta taaatgtttt agcaattgtt tcgtactatt
    52261 ttagattcag tctgctatat ataaccaatc tatcctaagt taactattta taatcggctc
    52321 tctgtaacaa aattaaatta attaatcccc ctataaaata aagaggttta gaatttgatg
    52381 tcttccaaac ctctttattt catatttaat gagatgctgc cttaaccgtg ataatatcta
    52441 atcatcaata aacaaactat ttgaataagg attctccatt tgattcaatc actcctttat
    52501 accaagtaaa ggacattttc ttatatcgat tgaatgtgcc atttccatca tcatttcgat
    52561 caacataaat gagaccgtac ctcttagaaa gctgtgcagt ggacatagaa acgcagtcaa
    52621 tacatcccca agacgtatag cccataattt ccacaccatc ttgtagagct tcagctactt
    52681 gcaataaatg ttctttcata tactgaattc tataatcatc ttggactgtt aagttattaa
    52741 attcatcttt tattagttga tctttagcac ctaatccgtt ttccactata aataatggaa
    52801 tttgataacg gtcataatat ctatttaaaa ttatacgtag tccaattgga tcaatttgcc
    52861 atccccactc agaagactct aaataaggat ttactaaacc accaataata ttcccttctc
    52921 ctgaattata ctgtgttgga agagcagatt gagttacact catgtaatag ctaaaggata
    52981 aaaatctaca gtataatttt ttaataactc tgcatcttca gctgcaaact ctatgttaat
    53041 gtcatgttcc ttaaaatatc tttttgcata attcggataa taacctctaa catgcacatc
    53101 tgaaaataga taatttagat tctcatactc atgagtcgcc catacatctt ttggatttgg
    53161 agtcattgga taagctggca tagctaatac catacacccc accttaaact ctgaattaat
    53221 ctcacgagca atttttgtaa ccaaactcga ggcgactaat tcatgatgta tagcttgata
    53281 taattcttgt ttcgaaagat tctccttagg tatatctatt cctccactag taaacggtaa
    53341 ttccaaaaca gagtttactt cgttaaatgt aagccaatat ttaactttat ctttatacct
    53401 ttctaaaact gttcgagcaa atttttcata aaaatgaatc attctcctat caatccatcc
    53461 atgatatttt cttgctaaat ataatggagt ctcatagtgt gaaagagtta caagtggttc
    53521 tatcccgtga gcatgtagtt catcaaacaa ttcatcataa tatttcaacc cagcttcgtt
    53581 aggttcttcc tcatctcctt ttggaaaaat tctactccat gcaatagaag tacgaaaaac
    53641 attaaagccc atttcagaaa acaaggatat atcttcctta tatttatgat aaaaatcaat
    53701 acctatcaat tttaagttat cttctgtagg attttctgtt gcttctccta atccaccttt
    53761 gggtaacaca tcctgaactg ataagccctt accatcttca ttatatgctc cctctacttg
    53821 attagctgca acagctccac cccaaagaaa atcatctgga aaaatggtca taactttcct
    53881 ccattataat attaccagta attccttaga atgctcgatt gtctgattat taggtaatat
    53941 taatacatct agaaaatcat tggtattcgt tacaattact ggtgtaactg tttcgtagcc
    54001 tttagtcttg attaaattca agtccatttc aaaaatcaac tgatttttga aaactctgtc
    54061 tccttcttct acatgactaa taaaaccttg accttttagc tcaacagtat ctaatccaat
    54121 atgaattagt aactcaacac cctcatcact cttcaatcca attgcgtgct tagtcggaaa
    54181 aatatttgta attttcccat caaatggtgc ataaacctta ccttcacttg ggataatcgc
    54241 tactccgtct ccaattagtt tatctgaaaa tgttttatcc tggacatcgc ttaacggaat
    54301 gatttctcct gatataggag aaaatatcat ttttttattt gaaactccag ctttaacttc
    54361 taaattgcta gaactctctt cttcatcgat tccaaatata taagctaata caaaggtaat
    54421 aacaaccgaa ataaccgcca caattaaagc atttacaata tttgatggta catcagaata
    54481 aataaattga ggcaacgcta tcaaagatgg gacagcaaat agatacgctt taacactagt
    54541 aagacctgca aataatcccg ctaatccacc accaatcata gctgcataaa gcggtttttt
    54601 atattttaaa gtcacaccat ataatgcagg ttcggtaatc cctgcaagta aggctgagaa
    54661 acctgctgca aaagcaattt gttttgtatt attattttta ctctttaatg caacagccat
    54721 cgaagcagcc ccttgagcta agtttgaccc taacattgct ggaagaatta atacgtctgg
    54781 agtagcaata gatgccgcca aaaaaatagg tgcaaaagcc caatgcattc cagtcataac
    54841 aataaatggc ataatagcac caagaatagc taatgtaagc catccagcta caccatacat
    54901 ttgcccaact agatttgata atccttcacc aacaattact ccaataggtc cgactacaac
    54961 taaggcaata cagcttgata ctaataatac tagcgtaggt tgcaaaaaac tcttagtaac
    55021 agctggtgtt aatttagcaa tcattttttc aatatatttc atcaaccaaa ccataataag
    55081 aattggaacg actgacgaac cataactagc tggtgtcaca ggtgcaccaa ataaactaag
    55141 aggattccct gattgcacca tttgaacaaa atttggatgg agaagtacac ctgctacaga
    55201 catagctaat gtagatgtta cttttaattt ttgtgatgca gaataagcta ataacagcgg
    55261 taagaaataa tatggagcat ccccaaaaaa tgtcaaaaaa gcaatagtct gagaatctga
    55321 ttgcaatata ccaagcattg gtaaaataat taccaagact ttcagcatac ctccccctaa
    55381 cattgctgga atgattggag tcatggaacc agcgatatat tcaatgattc tttctaaaat
    55441 attccctttg tgcccttgaa caactgaatc ggattcaaaa ttgccaagtt taacgaattc
    55501 tttataataa ttagctacat cattaccaag tataatttga tattgtccat tctttttcat
    55561 aatacctatt acacctggta tcttcttcac atcatcatca ttgactaaat tttcatcttt
    55621 taattctaat cttaaacgtg ttacacaatg ggtaactcta ttgacatttt tttcacctcc
    55681 aattacatcg aggatttttt gtaccgtatc tttataactc atggtattct cctattctat
    55741 taatctaaat tttttgttaa gcgacgaata tgagccatca aataaactaa ttcactagaa
    55801 gtcaacaaat aattgtactc agtttgtata aacattgcca cctgttcacc acattcatat
    55861 tctctaggat atttattttt cattaatgct aacaagtctt catcatcatc gtcacaataa
    55921 gttgtatttt caaccaagcg atgggcaaat aattttatat gagttatgaa tctataataa
    55981 tcaatagaat cttggttaaa ctcagtacaa tagaaatctt gaatgatttt ctcaattccc
    56041 ataattattt cagtgatttt atacgactct tgaaaattat tttctaaatt agcattaaca
    56101 aaatgtagcg ctatgaatgc agcttcgtca attggaagtt caatacctaa ctcttttttt
    56161 attatttgta aagccctttt cccaagttca aattcttctt tataatattt cgagatttcc
    56221 cagcgtaggg gattcgaaat aataactcct tccttataac gttctatgct cgaagaaata
    56281 tgatccgtta aattaatata gataatttca tcaaaattat tacccaatgt tattttacct
    56341 aggttaatta ttttttcggt gatgtacacc aactccaaag gaacgttaat aaacatctct
    56401 gtaaaacggg tcatattatc tctatttttg agaaaaaaac ttttctcaat atcaattgga
    56461 tttactttat ctccaatttt tcttccaaaa gctattcccc ttccaaaaag aagaatatcg
    56521 atatcttttt tactttgcgc aattacggca ttgtggttta gtattcgttt tatgatcata
    56581 aaatttccct tttaaaataa aaggaaccta cttaaaaaac atcaagaaac ttcttctgat
    56641 gtgattaaat aggttcctgc tcacttgtat tagtgataac atccttttat ttcaattttt
    56701 aatggtgaca tgaagcttaa ataatcaata actctgtaac cgtttttgtt ttatgatatt
    56761 tcaatctatt tgtcaagtac ttataaaaat ttttttattt tgtgtgattc gaatgtgata
    56821 atggcaacag tctcactaaa gaaatgaaaa tcatacatag aaatttttaa aaattttctc
    56881 tatcgtaaac ctgccactag ttcagaaact atactagctg attccaaacg taatatggtc
    56941 atctcatcta ccttccctaa taatggcatt gcaccatacc aaacaatagt cttattcacc
    57001 aaaagaatag gcgttactat ttttgattga gtaaaagaca ctttaatttt ctggctcttc
    57061 aactggtcta gccatgcttt atttgcaacc gtctctggta tacatatctc aatttgacta
    57121 atcctaaatt cttttagaaa attcatcagt tttgtctggt gcacataagg taaaataagc
    57181 agacattcct gtctttcccc tgctaagtcc tctctcaaca ccttctcata tctactatca
    57241 acataaacga attgtttctc ctcaccctca atggcacgat aatccatctt tcgataagct
    57301 acttgtcgtt tctgaaacat cttttctaaa taaggaacat gaatatccac ataatcgaaa
    57361 atccgcacca aagacttatc cttgtagttt ctatgaatcc gacctgagta ctgaatcaaa
    57421 ttatttttcc aagaaaaggg tgctgccaag ataagcgtgt ccaactgagg taagtcaaaa
    57481 ccttcgccaa tgtattttcc agtagacaac aaaacaaacc ctttatctaa ctgttctaac
    57541 gtctccagta aactcgttct ctttctcgga ctttggtttt tccgctaata atgtaacagt
    57601 catcaacctc tttctctttc aataactttt caaagacatc tatctgttga attcgattaa
    57661 ctaaaaccaa gatattccgt ccttctgcca cttgggctag aatatccttg agaatcaact
    57721 gattcctcgc tgagtctgta gcaatccaat cactgagctg tataaaatta cttgctttgg
    57781 tcttttcaat ttctctgaaa aacaataggt tcatgaccat tcttacgctc aggcgtagcc
    57841 gtcaaaccgt aaagatactt ccctctaaac tgagcaacaa ctttttcaaa catcaaggca
    57901 gagatatgat gacactcatc cacaatcatc atctcatact catccaaaag actttgacta
    57961 ttttccaact taaatagaga ttgaatcata acaacatcaa ccagtttact cagtcatttc
    58021 ttagccccac cgtactgccc gacatagcct attacccttt cacgacctga tggtgtataa
    58081 cgggtagctt ctgcctcttc gaaagtcaaa aaacacttta aacgatccag tcattggtct
    58141 aagagttgtc tattatgaac taggataatg gtttttgtct tacgttcaga gataagagca
    58201 gcacctaaaa cgatctttcc aaaacctgtc ccgcatgaag taaaccattt tctttagaat
    58261 tcatatctga cagggctaat tcttgctcca aagtaagctc tcccttaaat gctactctaa
    58321 tagacctttg tacttttctc ctatcctcca caactacctg cttaaattta tcttgcaatg
    58381 gatataacaa gcctcttggc agccataaat aataatcgga ttctccaaat aaatacattc
    58441 gctcaggaat ttgataggtt ggctgtcgca tagcctgctt taaatataat aaaatgaaat
    58501 aataacagga caaatcgatc aggaaagtca aatcgatttc taacaatgtt ttagaagcag
    58561 aggtatacta ttctagtttc aatctactat aaaattcggg attagaaaag gaagccatat
    58621 ttttcaagaa aaaaagtgcc ttggatgtca cagacgattt ttcaagttgg atcatattgg
    58681 ataaaacgac cttcaactcc ttatccaatt cttgcttgcc taactcctct tggattaaca
    58741 gtgccacttt agcagttgaa atcctctgaa tttcttgtag atacctccat tgatcttcat
    58801 aaggctgaaa atgttcatcc acaaagaccg ttcgcccttg atgataagct tctccttgaa
    58861 aaggaaggga aattaaatta tcaaatccac ctttaggaag aacatcctga tttggaaaca
    58921 tgccatcaaa agaatcaaag gacagttgca tactttcctg catagccaat tctaatagtt
    58981 tctttccaaa caggcgagcc tttcgactca gaatcgcttc ctcaaagaag aaccaaatat
    59041 ggagtccgta acccgaacga gaaatctcta aatgggcttc catctggcgt tcccttgcta
    59101 ttcttcgaat ggttaaacca gcttctttcc aatctccttc atccaaatcc agtaccaaaa
    59161 aataacagct atcatctaag tgcataggaa agataccgat agctgcctcc ccacgaaaat
    59221 gagatttcaa aacggagttc gtcaatgtct ggaaactccg tttttcaggt ggcaactgct
    59281 tccaaccata atcataggaa ggaaaatact gaattttccc ttgttcattg ataaaattct
    59341 tagcataaac atcataacgt ccagcaaaca cggaggtaaa caattgtaat ttttcttgcg
    59401 tcgtcaagtg actactttga agttggaaca tctcctcgaa ccgagagcgt tccatcacaa
    59461 actgactctt gtctttaaga cgtttacagt ggaatgaaat aagatatgaa caaattgatt
    59521 aggaaaatca aattaattta tagaaatctt ttagcagtca tggcgtccta ttccagcttc
    59581 aaaatgctat acacaagaaa aactcactat ccccattaaa agaaaaaaag acatcaagta
    59641 tttctaccac aattccatcc aaagacgcaa aaacagccat aagagtcacc tccttgattc
    59701 ctataggctg attataacaa gaatagctga aattgtacac gaaaataaaa tccgaatagc
    59761 actcattttg tatgcgacta atagtccatc tcgctccgga atgtacgaag taaatagagt
    59821 ttaaacgagc agtctataaa attcaaaaaa cgcatattat caagcgacct aaaactttga
    59881 tgatatacgt tttcttgtga gaatatttac ttcatttttg cctaaaattc aatgtttact
    59941 cagtatttgg attatgaaaa atcgaggtct aaatctagat acattttttc tgaagacaaa
    60001 tcattttgac caccgagcaa gagattttca aaaaaagctg ttaaaaactc aggacgtcgc
    60061 tgtaaaatct ttgcattatc taataccaag gcatcacgaa aatacttgga atgttgctga
    60121 aatggtatat tatcaatatc aaaaccaaac tcacgaagat actgaatcaa aaagaccgtt
    60181 actgtccgag tgtttccttc gcgaaatgga tgaatctgcc agattcctga aataaaatgc
    60241 tggatttgtt taaccacatc cgcctgagtt agtgtcgcat atgcaacttg tttttcctga
    60301 ttaaaatcat aatccaaggt catttgaatc atggagtaat cagagtacac aacactttca
    60361 ccattcaaaa caggttcatt ctttgtgata ttggtctgac gaaattgccc caccggaata
    60421 gagggttcaa atatatcttg aaacaactcc ttatgaatag caagtaaggt cgcaggacta
    60481 aatctaaaac ctcttcgaga caagagttct acgatacgca aagaaaccaa gtctgcctcc
    60541 tccgtactcg catcaacggt atgatgataa accgttgcat cctcataaac ctgctcataa
    60601 gtcagttcac cccgggactg tttctcagcc aaagattcca tatacgctga tggcactaga
    60661 ttgtcaactt tctgcagacc aaaacctatc cgccataaat cacgcttcgc ttcataagac
    60721 aagtttggat tgtcaatgtt gtaagttggt tgcatagaaa tatcctttca attgtttttc
    60781 tgtgtcatcg gtaaattgtg aaataggtat ggaattggcg aaaattataa aaatccttca
    60841 caataggttc tatgaaatat atatacttca tcttaatagt gagcaagcta aacttagcat
    60901 ttcatgccct catatgggat gttctttgac taaataatat gattatcgag atatatctgg
    60961 ataaatgaac taataagtct gacgcgtaga cttatcaaag tcattggcat acaccactat
    61021 gaactcgttg gtctgttcaa atcccaacac attacctgag aagaaagttg caatgttgtt
    61081 tttggtgcgg gtttgaattt aaaaaatttg ttatgtagta cctaatctaa ggaattagaa
    61141 caatgcctct aatttttctt taatacactg aaacattgat gattctggct gtatttttga
    61201 aacagctctt ctttgctcct ggaaaatatc ttcagaagtt atattctcta ttcctaacgc
    61261 tacttgagtt ttttttctaa aatattcttt tccgttgcca tctttagaaa aatcataacc
    61321 ttccctatct acgctgttac acaaattagc taaaaaaaac tctggggttg ggaaaggaag
    61381 ataagaaacg tatttagccc aataatctat aaagcttctt tgtttcgcaa ttaattcaat
    61441 attatttttt tgccctttat tacctgaaac attaaattta attggacatc ccgttatcaa
    61501 ttttataata tcatcaagat ttttattatc tgattcagga attttatctg atataacaac
    61561 accattttca agatagttca ttaaattatt tgattcacta acattagtgt tttgatctcc
    61621 atcaagccaa aaataatggt tatcggaatc taaatacgat gagtttaaaa tattattaca
    61681 aattatttga tttgctccac caggaatata tctcactact aaattctgtt taagattctc
    61741 actacctgaa tgagtgataa caaactctag aatatattta gctagtctat cttcaacata
    61801 aatcatcttc ctagaatgat acacatcacc taattcaaaa aatgcatcct gataatcaat
    61861 attttcaata acatctacct tttctccgtt tttcactaaa agtttcacgg cttctctagg
    61921 aaaatctttt ataa
//
LOCUS       DAVKQL010000002        55201 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVKQL010000002 DAVKQL010000000
VERSION     DAVKQL010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 55201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55201
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.002"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            74..1765
                     /locus_tag="VW561_000059"
     CDS             74..1765
                     /locus_tag="VW561_000059"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU9942410.1"
                     /translation="MNTKELIASKLSSIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            complement(2080..2638)
                     /locus_tag="VW561_000060"
                     /pseudo
     CDS             complement(2080..2638)
                     /locus_tag="VW561_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2600..2821
                     /locus_tag="VW561_000061"
     CDS             2600..2821
                     /locus_tag="VW561_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU9942411.1"
                     /translation="MNKSYFNPLAPTDKEDLIRTLELAPHVEGGYFRQTKKASDTINL
                     AGKGRALYTSIYFLLRRNESFTFSPIDSR"
     gene            2808..3110
                     /locus_tag="VW561_000062"
     CDS             2808..3110
                     /locus_tag="VW561_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU9942412.1"
                     /translation="MTADEIWYFHAGSPLTVHMITADGHYEVVTLGLDISKGQQLHYC
                     VPKGTIWGSTVDKDYALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTRY"
     gene            3194..3901
                     /locus_tag="VW561_000063"
     CDS             3194..3901
                     /locus_tag="VW561_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9942413.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            3902..5233
                     /locus_tag="VW561_000064"
     CDS             3902..5233
                     /locus_tag="VW561_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEU9942414.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            5400..6275
                     /locus_tag="VW561_000065"
     CDS             5400..6275
                     /locus_tag="VW561_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEU9942415.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            6393..7256
                     /gene="pstC"
                     /locus_tag="VW561_000066"
     CDS             6393..7256
                     /gene="pstC"
                     /locus_tag="VW561_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU9942416.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFIANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            7249..8064
                     /gene="pstA"
                     /locus_tag="VW561_000067"
     CDS             7249..8064
                     /gene="pstA"
                     /locus_tag="VW561_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU9942417.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            8066..8818
                     /gene="pstB"
                     /locus_tag="VW561_000068"
     CDS             8066..8818
                     /gene="pstB"
                     /locus_tag="VW561_000068"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9942418.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            8833..9483
                     /gene="phoU"
                     /locus_tag="VW561_000069"
     CDS             8833..9483
                     /gene="phoU"
                     /locus_tag="VW561_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU9942419.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELACARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            9551..9886
                     /locus_tag="VW561_000070"
     CDS             9551..9886
                     /locus_tag="VW561_000070"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9942420.1"
                     /translation="MNNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQF"
     gene            complement(10150..10530)
                     /locus_tag="VW561_000071"
     CDS             complement(10150..10530)
                     /locus_tag="VW561_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9942421.1"
                     /translation="MREFGEKIKRLRLAKKISRSEFCGDESELSIRQLIRIENGESRP
                     TLTKLKYIAERLGVEDYKLMPSYIELDKEYLELKYFLMRTPTYEDETIAQKKESVLIR
                     FLKSIMIGYLRKKDLSSQIIHIWH"
     gene            10742..11758
                     /locus_tag="VW561_000072"
     CDS             10742..11758
                     /locus_tag="VW561_000072"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU9942422.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKNVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            11780..12679
                     /gene="galU"
                     /locus_tag="VW561_000073"
     CDS             11780..12679
                     /gene="galU"
                     /locus_tag="VW561_000073"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEU9942423.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDETTGMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FEILEKQAPGAGNEIQLTDAIDTLNKTQRVFAREFTGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(12746..13423)
                     /locus_tag="VW561_000074"
     CDS             complement(12746..13423)
                     /locus_tag="VW561_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU9942424.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIILRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(13407..13946)
                     /locus_tag="VW561_000075"
     CDS             complement(13407..13946)
                     /locus_tag="VW561_000075"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEU9942425.1"
                     /translation="MKSELRKQVLQGMKAISQEQKQFIDQTLTERLLHHPFYQEAKVI
                     ATYLSFPHEFQTQELIEQALKDGKKVLIPKTYPKGRMDFVVYDPQQLVKTSFGLLEPQ
                     GDLEVVDASQIDLIHVPGLAFTTKGYRIGYGGGYYDRYLEHFSGHTLSTVHPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            complement(13958..15088)
                     /locus_tag="VW561_000076"
     CDS             complement(13958..15088)
                     /locus_tag="VW561_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEU9942426.1"
                     /translation="MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEAGAFGDWLPDQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            complement(15156..15854)
                     /gene="dapD"
                     /locus_tag="VW561_000077"
     CDS             complement(15156..15854)
                     /gene="dapD"
                     /locus_tag="VW561_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEU9942427.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNVVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            complement(16084..16995)
                     /locus_tag="VW561_000078"
     CDS             complement(16084..16995)
                     /locus_tag="VW561_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU9942428.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVSVISASEPLSSALLSVLFLGLVV
                     DWSFLLAIALIILPMIFLSIEEAKESR"
     gene            complement(17060..19525)
                     /locus_tag="VW561_000079"
     CDS             complement(17060..19525)
                     /locus_tag="VW561_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU9942429.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGVGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            19667..20923
                     /locus_tag="VW561_000080"
     CDS             19667..20923
                     /locus_tag="VW561_000080"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU9942430.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEESYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADEHNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            complement(21076..21549)
                     /gene="tnpA"
                     /locus_tag="VW561_000081"
     CDS             complement(21076..21549)
                     /gene="tnpA"
                     /locus_tag="VW561_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISSpn6 family
                     transposase"
                     /protein_id="HEU9942431.1"
                     /translation="MREDNQSLSHTTWNCKYHIVFAPKYRRQIIYGRYKASIGRIIRD
                     LCERKGVIIHEANACSDHIHMLISIPPKLSVSSFMGYLKGKSSLMIFDKHANLKYKYG
                     NRKFWCRGYYVDTVGRNQKVIAEYIQNQLQEDRVADQLTLFESVDPFTGEINKRK"
     gene            complement(21740..23803)
                     /locus_tag="VW561_000082"
     CDS             complement(21740..23803)
                     /locus_tag="VW561_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU9942432.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRGFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWLSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(23805..24083)
                     /locus_tag="VW561_000083"
     CDS             complement(23805..24083)
                     /locus_tag="VW561_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942433.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            24235..25083
                     /locus_tag="VW561_000084"
     CDS             24235..25083
                     /locus_tag="VW561_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU9942434.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            25706..26278
                     /locus_tag="VW561_000085"
     CDS             25706..26278
                     /locus_tag="VW561_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942435.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALA
                     FAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
     gene            complement(27322..29580)
                     /locus_tag="VW561_000086"
     CDS             complement(27322..29580)
                     /locus_tag="VW561_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEU9942436.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIDLDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(29606..31123)
                     /gene="malQ"
                     /locus_tag="VW561_000087"
     CDS             complement(29606..31123)
                     /gene="malQ"
                     /locus_tag="VW561_000087"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEU9942437.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEYFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWQIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            31674..32945
                     /locus_tag="VW561_000088"
     CDS             31674..32945
                     /locus_tag="VW561_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9942438.1"
                     /translation="MSSKFMKSTAVLGTVTLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIVGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            33056..34348
                     /locus_tag="VW561_000089"
     CDS             33056..34348
                     /locus_tag="VW561_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9942439.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            34350..35192
                     /locus_tag="VW561_000090"
     CDS             34350..35192
                     /locus_tag="VW561_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9942440.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            35445..36245
                     /locus_tag="VW561_000091"
     CDS             35445..36245
                     /locus_tag="VW561_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEU9942441.1"
                     /translation="MLPYPFSFFSSILGFRKPLSKRFGLNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTVSQASSVVIG
                     PSQIKELPKNLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            36255..37241
                     /locus_tag="VW561_000092"
     CDS             36255..37241
                     /locus_tag="VW561_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9942442.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            37377..37457
                     /locus_tag="VW561_000093"
                     /pseudo
     CDS             37377..37457
                     /locus_tag="VW561_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(37577..38518)
                     /locus_tag="VW561_000094"
     CDS             complement(37577..38518)
                     /locus_tag="VW561_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU9942443.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(38496..40259)
                     /gene="aspS"
                     /locus_tag="VW561_000095"
     CDS             complement(38496..40259)
                     /gene="aspS"
                     /locus_tag="VW561_000095"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEU9942444.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEALAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSAEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
     gene            complement(40494..40574)
                     /locus_tag="VW561_000096"
                     /pseudo
     CDS             complement(40494..40574)
                     /locus_tag="VW561_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(40610..40840)
                     /locus_tag="VW561_000097"
     CDS             complement(40610..40840)
                     /locus_tag="VW561_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942445.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            complement(40858..41499)
                     /locus_tag="VW561_000098"
     CDS             complement(40858..41499)
                     /locus_tag="VW561_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9942446.1"
                     /translation="MKLLKNLGWILLALLSFLFIYGFIQGLATASLALGASPYAVTLL
                     YVALAVVYVYGIYKWYQKAPVHIEKSGFNRFIWLPVLVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            complement(41511..42245)
                     /locus_tag="VW561_000099"
     CDS             complement(41511..42245)
                     /locus_tag="VW561_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEU9942447.1"
                     /translation="MKKKRRLLFLMSIVLGGFLGMFVGMFKARVESHEIILDVKALMP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIILSFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            complement(42248..42445)
                     /locus_tag="VW561_000100"
     CDS             complement(42248..42445)
                     /locus_tag="VW561_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942448.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            complement(42445..42651)
                     /locus_tag="VW561_000101"
     CDS             complement(42445..42651)
                     /locus_tag="VW561_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9942449.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            complement(42661..42975)
                     /locus_tag="VW561_000102"
     CDS             complement(42661..42975)
                     /locus_tag="VW561_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942450.1"
                     /translation="MWVLVFILFIIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRL
                     LQEMIGKKPIITGVYIGPDNWEVVDVDEEWVKLRRVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            complement(43230..44519)
                     /locus_tag="VW561_000103"
     CDS             complement(43230..44519)
                     /locus_tag="VW561_000103"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEU9942451.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQTHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     RQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(44597..45803)
                     /locus_tag="VW561_000104"
                     /pseudo
     CDS             complement(44597..45803)
                     /locus_tag="VW561_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
     gene            complement(45994..46845)
                     /gene="rgg"
                     /locus_tag="VW561_000105"
     CDS             complement(45994..46845)
                     /gene="rgg"
                     /locus_tag="VW561_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398326.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEU9942452.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIIIKSYMKSIDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRKTLDS"
     gene            46969..47310
                     /locus_tag="VW561_000106"
     CDS             46969..47310
                     /locus_tag="VW561_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU9942453.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            47368..47448
                     /locus_tag="VW561_000107"
                     /pseudo
     CDS             47368..47448
                     /locus_tag="VW561_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(47789..49492)
                     /gene="ilvD"
                     /locus_tag="VW561_000108"
     CDS             complement(47789..49492)
                     /gene="ilvD"
                     /locus_tag="VW561_000108"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU9942454.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETPIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(49725..50657)
                     /locus_tag="VW561_000109"
     CDS             complement(49725..50657)
                     /locus_tag="VW561_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEU9942455.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSDSDIVVLEADLSSSMATHNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFSSRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEFYNAR"
     gene            complement(50654..51511)
                     /locus_tag="VW561_000110"
     CDS             complement(50654..51511)
                     /locus_tag="VW561_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU9942456.1"
                     /translation="MILSKNREDGLRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEERQMLTSVVER
                     LSQELEETE"
     gene            complement(51515..52861)
                     /locus_tag="VW561_000111"
     CDS             complement(51515..52861)
                     /locus_tag="VW561_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9942457.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFVGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGILNQFGSQAGIVIGLVLILAVMFGVSFIKKPSATEE"
     gene            complement(52874..53158)
                     /locus_tag="VW561_000112"
     CDS             complement(52874..53158)
                     /locus_tag="VW561_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9942458.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKICEEKGLI"
     gene            complement(53162..55192)
                     /locus_tag="VW561_000113"
     CDS             complement(53162..55192)
                     /locus_tag="VW561_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEU9942459.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
BASE COUNT        16946 a        11858 c        10202 g        16195 t
ORIGIN      
        1 agatttcaaa aattttttct tctttcttta aaattgtgat aaaatagtag aaaagtccaa
       61 aaaggagctc ttaatgaata caaaagaatt gattgctagc aaattatcta gcatcattga
      121 tagcctggac caagaggcta ttttaaagtt actggaaacc cctaaaaact cagaaatggg
      181 agacattgct ttccctgctt tttctcttgc aaaagtcgaa cgtaaagcac cacaaatgat
      241 tgcggctgaa ctggctgaaa aaatgaacag ccaagccttt gaaaaagttg tcgcaacagg
      301 accttacgtt aactttttcc ttgataaatc tgccatttct gctcaagtat tgcaagctgt
      361 taccactgaa aaagaacact atgctgacca aaatattggt aaacaagaaa atgttgttat
      421 cgacatgtct agtccgaata tcgctaaacc attttctatt ggccacctgc gttcaactgt
      481 tatcggagat agcttgtcac atattttcca aaaaatcggt tatcaaacgg tcaaggtcaa
      541 ccatttggga gactggggta aacaatttgg gatgttgatt gttgcctaca aaaaatgggg
      601 cgacgaagaa gctgtaaaag ctcatccaat cgatgaactc cttaaactct atgtccgcat
      661 caacgctgaa gctgaaaatg accctagctt ggatgaagaa gcgcgcgaat ggttccgtaa
      721 acttgaaaat ggagatgagg aagctctcgc tctttggcaa tggttccgcg atgaaagttt
      781 agtggaattt aaccgccttt acaatgaatt gaaggttgaa tttgacagct ataacggaga
      841 agccttctac aatgataaga tggatgcagt tgtagacatt ctttctgaaa aaggactact
      901 tcttgaatca gaaggtgccc aagttgtgaa tcttgagaaa tatggaattg aacatccagc
      961 cctcatcaag aagtctgatg gtgcaactct ctatatcaca cgtgacttgg ctgcagccct
     1021 ctaccgtaaa aacgaatacc aatttgctaa atctatctat gtcgttggtc aagaacaatc
     1081 tgcccacttt aaacagctca aagctgtctt gcaagagatg ggctacgatt ggagtgacga
     1141 cattacccac gttccttttg gtctggttac aaaagaaggg aagaagctct ctactcgtaa
     1201 agggaatgtc atcttgctag agcctactgt tgcagaggct gttagccgtg ccaaggtcca
     1261 aatcgaggct aaaaatcctg aactagaaaa caaagaccaa gtagcacatg ctgttggggt
     1321 tggagccatt aaattctatg acctcaaaac cgaccgtaca aatggatacg acttcgacct
     1381 agaagctatg gtatccttcg agggtgaaac tggaccttac gttcaatatg cctacgctcg
     1441 tatccaatct atcttacgca aagccgattt caaaccagaa acagctggca actatagctt
     1501 gaatgatact gaaagctggg aaatcattaa actcattcaa gacttcccac gtattatcaa
     1561 ccgtgcggca gataactttg aaccttctat cattgctaaa tttgcaatta gcctggctca
     1621 atcctttaac aaatactatg cacatacacg tatcttggat gaaagcccag aacgcgacag
     1681 ccgtctagcc ctcagctacg caaccgcagt cgttctcaaa gaagcccttc gcttgcttgg
     1741 agtagaagcg ccagagaaga tgtaaatcgc caaagttact tgattgcaaa gcaatctaag
     1801 taactaacgc caaatcctat tcggactttg gctaggcgct tcactagttt taggacataa
     1861 atatgatcga tttatgtcct aaaacgtcgt aatcttaaag ttattggaac taaagttcct
     1921 aattgttaga cgctagccgc ttatatgcga actagctaac tgcttcacta gtcatgaccc
     1981 ctaaatataa tcgatttaga ggccatgacg tcgtaaccag agacacttgc ctaaacactt
     2041 cactaattca cctaaccaaa taatagactt cctgcaaaac tagaatccta gttcatgatt
     2101 gataatacca gcaatcaaat tcattcgtaa tccgaagcgt ttacgatgat ttcgataggt
     2161 tgttgaaaac attttaaacg tttttacttt ggcaaagatg ttctcaacct tgcttctctc
     2221 cttagatagc gcatggttat aggctttatc ttcagctgtt agtggcttga gtttgctgga
     2281 tttacgtgca gtttgtgctt gaggacatat cttcatgagc ccttgataac cactgtcagc
     2341 caagatttta ccagcttgtc cgatatttct gcaactcatt ttgaacaact tcatatcatg
     2401 acaatagttc acagtgatat ccaaagaaac aattctccct tgacttgtga caatcgcttg
     2461 agccttcata gcgtggcatt tctttttacc agaatcattc gctaattctt tttttagggc
     2521 gattgatttg tacttccgtc gcatcaatca ttaccgtgtc ctcagagctg agaggagttc
     2581 ttgaaatcgt aacaccactt tgaacaagag ttacttcaac ccattggctc cgacggataa
     2641 agaagacttg ataagaactc tagagttagc tccccatgtt gaaggtggct attttagaca
     2701 aacaaaaaaa gcttcagaca ctataaacct tgctggtaag ggaagggcgc tttataccag
     2761 tatttatttc ttattaagaa gaaacgaatc cttcacattt tcaccgattg acagcagatg
     2821 aaatctggta ttttcatgca ggatccccac ttactgttca catgattaca gcagatggtc
     2881 actacgaagt agtcacttta ggcctggaca tatccaaagg gcaacaactt cactactgtg
     2941 ttcctaaggg cactatttgg ggatcaacag ttgataagga ttatgctctg gtttcttgtc
     3001 ttgttgctcc tggttttgaa tttgaggatt ttgaattatt tgaaagggta gatctactgg
     3061 cgacctatcc agagcacaag gagatgattg agcgtttgac cagatactag tcttgataag
     3121 caaatttaat ctgatttagc aatgttagga tttctgataa ttgtgttata atgttaccaa
     3181 taggagataa tagatgacaa aacaagtctt attagtggat gatgaagaac acattctgaa
     3241 attgcttgac taccatttaa gtaaggaagg cttttctact caattggtaa caaatggacg
     3301 gaaggcctta gctttggcag aaacagaacc ctttgatttt atcttgcttg atatcatgtt
     3361 accacaatta gatggcatgg aagtttgtaa gcggctgaga gccaaaggcg tcaaaactcc
     3421 aattatgatg gtttctgcga aaagtgatga atttgataag gttttggcct tggaattagg
     3481 ggctgatgac tacctgacca agccttttag ccctagagaa ttgctggcgc gtgtcaaggc
     3541 tgtcctcagg cgaactaaag gagaacaaga aggagatgat tcagataata tcgctgacga
     3601 ttcttggcta tttgggacct tgaaagtata ccctgagcgt catgaagtct acaaggcgaa
     3661 taagttactg agtttgaccc caaaagaatt tgaactcttg ctctatctta tgaaacatcc
     3721 caacatgaca ctgactagag agcgtctttt ggaacgtatc tgggggtatg actttgggca
     3781 ggaaacacgt ttggtggacg ttcatattgg taagttgagg gaaaaaattg aagacaatcc
     3841 taaagcccct caatttattc gaaccattcg gggttatggt tataagttca aggagttata
     3901 gatgaaacgc taccttcaat tttggctagt caatctaagt gttagcctca ttctgattgc
     3961 agggatggca ttaacttgga tcagtaaagg catcggtctc tttcttctag ccctttcttt
     4021 gggacaaggt ggctactggc tcttttgcct ttggaaatgg gaagttgctt ttgagacctt
     4081 gcaccaacct ctcttgacca gtagtgaata ctttttagaa aaagggcaag aagacttaaa
     4141 gtctttggct cagtatgtgt ctggcctaaa aaccaaagtc tcccaacagg atcagcaata
     4201 caaggaccta gccgaaacaa tggaggttct tctgtcgcac ctaaccatgg ggacgttttt
     4261 agtttccgct caaggtcaga tgttattatc cagtcgctct ctgcctcatt attttcccga
     4321 tgtggacggc gacataagtt cacttgatga cctcaaacgg atggatattc ggaatttagt
     4381 tcaccaagcc tttgatcaaa aaacaaggtt aaaacaagaa gtaagtgggt ttcatgaggg
     4441 tgacttgatt ttagaagtga cagcagttcc cgtttttagc cccacccaat ctgtggaagc
     4501 tgtgctcgtc ttgctatatg atttaacaac gattagaact tatgaaaagt taaatttagc
     4561 ctttgtctca aatgcctccc atgaattgag gacaccagtt acttcaatta agggctttgc
     4621 tgaaaccatt aaggggatgt cagctgaaga agaagcgctc aaggatgact ttctagacat
     4681 tatttacaaa gaaagtttgc gtcttgagca tattgttgag catcttctta ccttatctaa
     4741 ggctcaacaa atgcctatac aatggacgac cctttctttg gcagaatttg tacaggattt
     4801 gactcaaagc ttgcaacctc agctcaagaa gaaggattta cagctaaagg tgcaggtgcc
     4861 agatgatgtc accctcgtat cagatagtca attactttcc caaatcttac tcaatctttt
     4921 atccaatgcc atccgttaca ctgaacaagg gggaaaaatt gaggtcaaga cccaaaaggt
     4981 gaacgaaggc attaagattt ctgtatcaga tacagggatt ggtattagtc aattagagca
     5041 ggatcgtatt tttgaacgtt tttaccgagt taataaaggt cgaagcagac aaactggtgg
     5101 cactggtctt ggccttgcca ttgtcaaaga actcagtcaa ttattaggtg gccaagtcac
     5161 ggtgacgagt cagcttggca gaggcagttg cttcacgatt tttcttccta accaatcttt
     5221 cgcacaggac taataatttt taaagctatt tttaggagca gccatttttc aaaagtggct
     5281 ttttttgatt ctttacacaa tctttacatg aatcagggat aaacctttac aaagacttgt
     5341 tagactataa atgtagtaag cctacacaag aaaaatacat agagataaag gtgattatta
     5401 tgaaattcaa aaaaatgctt actcttgcag ccattggctt atcaggattt gggcttgttg
     5461 cctgtggcaa tcagtcagct gcttccaaac agtcagcttc aggaacgatt gaggtgattt
     5521 cacgagaaaa tggctctgga acacggggtg ccttcacaga aatcacaggg attctcaaaa
     5581 aagacggtga taaaaaaatt gacaacactg ccaaaacagc tgtgattcaa aatagtacag
     5641 aaggtgttct ctcagcagtt caagggaatg ctaatgctat cggctacatc tccttgggat
     5701 ctttaacgaa atctgtcaag gctttagaga ttgatggtgt caaggctagt cgagacacag
     5761 ttttagatgg tgaataccct cttcaacgtc ccttcaacat tgtttggtct tctaatcttt
     5821 ccaagctagg tcaagatttt atcagcttta tccactccaa acaaggtcaa caagtggtca
     5881 cagataataa atttattgaa gctaaaaccg aaaccacaga atatacaagc caacacttat
     5941 caggcaagtt gtctgttgta ggttccactt cagtatcttc tttaatggaa aaattagcag
     6001 aagcttataa aaaagaaaat ccagaagtta cgattgatat tacctctaat gggtcttcag
     6061 caggtattac cgctgttaag gagaaaaccg ctgatattgg tatggtttct agggaattaa
     6121 ctcctgaaga aggtaagagt ctcacccatg atgctattgc tttagacggt attgctgttg
     6181 tggtcaataa tgacaataag gcaagccaag tcagtatggc tgaacttgca gacgttttta
     6241 gtggcaaatt aaccacctgg gacaagatta aataaaatgt ttgctccata aatctctaaa
     6301 gagatgcaga cgtttcatcg tacaataaga taaagaaggc aagtagggag gtgtcgtatc
     6361 tcccttactt tcttcactag aaaggacaag atgtgacaaa acaagccttc aaagaagcag
     6421 tttttagggc aatttttttc atgagtgcaa cagtagctgt tgtagctatt ttgctaatct
     6481 gtttctttat ttttagtaat ggcttacctt tcatagctaa ctacggcttt gcccgttttt
     6541 tattaggcag tgattggtcg ccaacgaaca ttccggcaag ctatggtatt ttaccaatga
     6601 tcgttggttc cttattaatt accttaggag cgattgtgat tggggtgcca acaggcatct
     6661 tgacatcggt gtttatggtt tattattgtc caaagcccgt ctatggcttc ttaaaatcag
     6721 ctatcaactt gatggcagcc attccatcta ttgtttatgg ttttttcggc ctacaattat
     6781 tggtgccttg gattagaagc tttttaggaa atggcatgag tgtcctaacc gcttcgttac
     6841 tattaggaat aatgattttg ccaaccatta tcagtttgtc agaatctgct atccgaacag
     6901 ttcccaaaac gtattattct ggtagcttgg ctctaggagc tagtcatgaa cggagtattt
     6961 ttagtgtcat cttgccagct gcgagatctg gtattttatc agcagttatt ttaggaatcg
     7021 gtcgcgcagt aggtgaaacc atggcagtta ttttggtggc aggcaaccag ccgattattc
     7081 caagtggact cttttcagga accagaacct taacaaccaa tattgttctg gaaatggctt
     7141 acgcatcagg tcagcatagg gaagccctta ttgcaacctc agcagttctc tttttcctta
     7201 ttctcttgat taatgcctac tttgcctact tgaaaggaaa atcatcttat gagtaaatac
     7261 ctgctaaaac ttctcgttta ttgtttttca gctttaacct ttggctctct ctttttaatc
     7321 attggtttta tcctcatcaa aggcttacct catctaagtc tatccctctt ttcttggact
     7381 tatacttctg agaacatttc ccttatgcca gcgattattt ccaccgttat tctggtcttt
     7441 ggtgctcttc ttttagcctt gcccataggg atttttgctg gtttttatct tgtggaatat
     7501 acaaaaaaag attccctttg tgttaaaatc atgcgattgg cctcagatac cttatctggg
     7561 attccttcca ttgtttttgg tctgtttggc atgctcttct ttgtagtctt cttaggtttt
     7621 caatactctc tgttatcagg aatcttaacc tcagttatca tggtgttgcc agtcattatt
     7681 cgctcaacag aagaagccct tttatctgtt agtgatagta tgcgtcaagc aagttatgga
     7741 cttggggcag gtaagttacg gactgttttt agaattgttc taccagttgc catgccaggt
     7801 attttagctg gagtgatact agctattggc cgtatcgttg gtgaaacagc tgccctcatg
     7861 tatacattag gtacctctac caatacgcca agtagtctca tgtcttcagg ccgttctcta
     7921 gccctacata tgtatatgct gtcaagtgag gggctacatg tcaatgaagc ctatgctacc
     7981 ggcgtgattt tgattattac tgttttaatg ataaatactc tatcaagctt attatctcgg
     8041 aaacttgtga aaggagcttc ctagtatggg aacattttca gttagacacc tagacttatt
     8101 ttacggggat tttcaagcct taaaaaatat ttcgattcaa ttaccagaaa gacagattac
     8161 tgccttgata ggcccatctg gttgtggcaa atcaactttt ctaaaaaccc ttaatcggat
     8221 gaacgatttg gttccttctt gccatattga aggccaagtc ctcttagatg agcaagatat
     8281 ttatagtagc aaattcaacc ttaatcagct acgcaagcgt gtagggatgg tttttcaaca
     8341 gcctaatccc tttgccatgt ctatctatga taacgtggct tatggtccaa ggacacatgg
     8401 tattcgagac aaaaaacaat tagatgcctt agtggagaaa tctttaaaag gggcagccat
     8461 ttgggaagaa gtcaaagatg atcttaaaaa gagtgccatg tccttatctg gcggtcagca
     8521 gcaacgcctt tgcattgcgc gagctttagc agtagaacct gatattctgt taatggatga
     8581 gccgacttca gccttagacc ctatctccac tttaaaaatt gaagacctca ttcagcaact
     8641 aaaaaaggat tatacgatta tcattgttac ccataacatg caacaagctt cacgtatttc
     8701 agataaaact gcttttttct taacaggaga aatttgcgaa tttggagata ccgttgacgt
     8761 gtttaccaat ccaaaagatc agcgcacaga agactatatt tcaggacggt tcggataagg
     8821 aaggaaaaac ctatgagaaa tcaatttgac ttagaattgc atgaattaga acaatccttt
     8881 ttaggactag ggcaacttgt ccttgaaaca gcttcaaaag ccttactggc cttagcctcc
     8941 aaagacaagg agatggcaga gctaattatc aataaggatc atgctatcaa ccaaggtcaa
     9001 agcgctatcg aattggcctg tgcccgtttg ttagccttgc agcagccaca agtgtctgac
     9061 cttcgatttg taattagcat catgtcttct tgttcagacc ttgaacgtat gggagaccat
     9121 atggcaggca ttgccaaagc tgttttgcaa ctaaaagaaa atcaactagc ccctgacgaa
     9181 gaacagttac accaaatggg taaattatcc ctcagcatgc tagccgattt attggttgcc
     9241 tttcctttgc accaagcctc aaaagctatt agtattgctc aaaaagatga acagattgac
     9301 caatattatt atgccttatc aaaggaaatc attggactta tgaaagacca agaaacctca
     9361 attcccaatg gaacacaata cctttatatc atagggcatc tggaacgctt cgctgattac
     9421 attgctaaca tttgtgaacg cctagtctac ctagaaacag gagaactagt ggatttgaat
     9481 taattcaact aatccttaaa agagaagagt acgattaagt gctctttttt atggttgtaa
     9541 aaaagttcat ttgaacaatt taagcagtgt agatagtgag gagctgtttc aattctatcg
     9601 tgaacgaggg aatgctgaaa actttatcaa agaaaggaaa gcaggattct ttggggataa
     9661 gacagatagt tcgaccatga ttaagaatga agtacgtatg atgatgggct gtctggctta
     9721 taatctctac ctctttttaa agcagctagc tggtgatgaa gtaaaggcct tgactatcaa
     9781 gcgttttcga cgtctcttcc ttcatattgc cggaaaatat gtctctactg ctagacgaca
     9841 tattctcaaa ttctcaagtc tatatgccta ttcaaaacag ttttaagcct tatttgatac
     9901 aatctgccag ataaatctga tactccctgt tccatataga gctagagggc aggggaaaac
     9961 atgcctaaca gaataagtca ccttacttta aaaatcgagc atcaaaccaa gggaggagtc
    10021 tgcccttttt taggaaaaaa tcaagacaaa tctcctcaat catgtctcga acatcagaaa
    10081 ttaagcaaaa tcaccagaag gacagtattt caactagctt ttctggtaat ttttgaactg
    10141 tgtagttcgt tagtgccaga tatgaataat ttgggatgat aaatctttct tcctcaggta
    10201 gcctatcata atactcttca aaaatcttat caaaacgctc tctttctttt gggcgatagt
    10261 ttcatcttcg tatgtaggag tcctcatcaa gaaatacttc aattctaggt attccttatc
    10321 caactctata taacttggca tcaacttgta atcttcaacc cccaaacgtt cagcgatata
    10381 ttttaacttt gttagtgttg gtctggattc tccattttca attctaatta attgacggat
    10441 acttaattca gactcatcac cacaaaattc tgaacgactg atttttttag ccaaacgtaa
    10501 tcttttaatt ttttcgccaa actctcgcaa cctacaagaa cctcctgagt tgtttacctc
    10561 tatttatagc atatactgaa tcaaactatc tatcagattt cttctcactt taactaaaga
    10621 ctaagagttt atcccttcat ctcggttttt gtgtattttt ccaccatacc ccagtaatgc
    10681 aagtgcaaaa tcccctagaa tatgatagaa taagagaaag aactctatca aggaggaaat
    10741 catggaaaaa caaaccgtcg ccgtcttggg gcctggttct tggggaaccg ccctttcaca
    10801 agtcttaaat gacaatggac acgaggtacg tatttgggga aatcttcccg agcaaatcaa
    10861 tgaaattaat acacaccata ctaataagca ctactttaaa gatgtcgttc tagacgaaaa
    10921 tatcattgcc tacaccgact tagcagaaac attgaaaaat gtggatgcga ttttgtttgt
    10981 tgtcccaaca aaagtgacac gacttgttgc ccagcaagtt gcacaaacct tggaccataa
    11041 ggttatcatc atgcacgcat caaagggatt agaacctgat agccataaac gattatcaac
    11101 cattcttgaa gaagaaattc ctgaacatct ccgtagtgat atcgtcgttg tttcagggcc
    11161 tagtcatgca gaagagacca ttgtgcgtga cctaacttta ataactgctg cttctaaaga
    11221 tttacaaaca gctcaatacg ttcaggagct atttagtaat cactacttcc gactttatac
    11281 caatacggat gttatcgggg ttgaaactgc tggtgctctt aaaaatatta ttgctgtcgg
    11341 tgctggagct ttacatggtc ttggatttgg tgataatgct aaggcagcca tcatcgctcg
    11401 aggtttagca gaaatcaccc gcctaggggt agcactcggg gccagtccat tgacctatag
    11461 cggcttatct ggtgtgggag atttaatcgt aacaggaact tccatccact ctcgtaactg
    11521 gagagctgga gatgctctcg gacgaggaga atccctagct gatatagaag ctaatatggg
    11581 catggtaatc gaaggaattt caacgactcg agcagcctat gaactagccc aagaacttgg
    11641 agtctatatg cccattacac aggctattta ccaagttatt tatcacggaa ccaatatcaa
    11701 agatgccatt tatgacatca tgaacaatga atttaaagca gaaaatgagt ggtcttaacc
    11761 ctctatagaa aggattttta tgacatcaaa agttagaaag gcagttatcc ctgctgctgg
    11821 actaggaact cgatttttac cagcaaccaa ggcccttgcc aaagaaatgt tgccaatcgt
    11881 agacaaacca actatccagt ttatcgtgga agaggctctc aaatcaggta ttgaagatat
    11941 tctagttgtc actggtaaat caaaacgttc tattgaggac cacttcgatt caaacttcga
    12001 attggaatat aacctcaaag aaaaagggaa aacagatctt ttgaagctag ttgatgaaac
    12061 aactggcatg cgtctgcatt ttatccgcca aactcatcca cgcggtctcg gagatgctgt
    12121 tttgcaagcc aaggctttcg tcggaaatga accttttgtc gttatgcttg gtgatgactt
    12181 gatggatatc acagacgaaa aggctgttcc acttaccaaa caactcatgg atgactacga
    12241 gcgtacccac gcgtctacta tcgctgtcat gccagtccct catgacgaag tatctgctta
    12301 cggggttatt gctccgcaag gcgaaggaaa agatggtctt tacagtgttg aaacctttgt
    12361 tgaaaaacca gctccagagg acgctcctag cgaccttgct attatcggac gctacctcct
    12421 cacgcctgaa atttttgaga ttctcgaaaa gcaagctcct ggtgctggga atgaaatcca
    12481 acttactgat gcaatcgata ctcttaataa aacacagcgt gtttttgccc gtgaatttac
    12541 aggagctcgt tacgacgttg gggataagtt tggctttatg aaaacatcta ttgactacgc
    12601 cctcaaacat ccacaagtca aagatgattt gaagaattac ctcatccaac ttggaaaaga
    12661 attgactgag aaggaataaa ataaaaagat aaggttcaaa aaatgcctta tcttttttca
    12721 ttacacaaat tttctttcaa cttcgtcaca ttcccattcc cttgtagaaa agcaaaatgg
    12781 cgagtcctac gaacaagact accgctccta atctctggct ggtgttatac atccgttttt
    12841 ctcctctaac tggaaagata actgctagaa atgcgccacc aactgcacca ccgatatggc
    12901 ctgctaggct gattcctgga atcagaacac ttccaataat gttaaccaca aaaagtgtca
    12961 gataagattg ccctagctgt tggatataag gattgcgagt tgcatagcga agaatgataa
    13021 tcgcggcaaa tagcccataa agagaggtag aggcgcctgc tgctaaggat ttaggactaa
    13081 atacaaaaac aaagagattg cccatcattc ctgataaaag atagagaaag aaaaactgct
    13141 tagaaccgaa aatctcctct acctgccttc caagataata aagtgaaagc atattaacaa
    13201 tgaaatgttc ccacccaata tgaacaaaaa tggcagacaa gagacgccaa acctgctcgg
    13261 gaaagaggcg aatagctggc ccatacatgg ctccaaatcg aaataatgta tctgccctgt
    13321 caaagtttcc gcctgcagtg accaacatta gtaaaaatac caaggccgtc actaagagga
    13381 agaaactcgt cacagggtaa cgtctatcaa agatttcctt catcaattaa tacctcctga
    13441 acaggaatat catggttttc aggtataaag tcctgaattt gacaaggatg aaccgtactc
    13501 aaagtatgac cagaaaaatg ttccagatag cggtcataat agcctccacc gtatcctatc
    13561 cgatatcctt ttgtcgtaaa agccaggcca ggaacatgaa tcaaatcaat ctgagatgca
    13621 tccaccactt ccaaatctcc ctgtggctcc agtaatccaa aggaagtttt taccaactgt
    13681 tgcggatcat agaccacaaa gtccatgcgc cccttgggat aagttttggg tattaaaacc
    13741 ttcttgccgt ccttcagcgc ctgctcaatc agttcctgcg tttgaaactc atgaggaaaa
    13801 gagagatagg ttgcgatgac cttggcttct tgatagaagg gatggtgtaa aagccgctcg
    13861 gttaaagttt gatctataaa ctgtttttgt tcttgagata tagccttcat tccttgcaag
    13921 acttgcttgc gtaattccga tttcatagac aagccctcta ttctgctgcc ttctttttca
    13981 ggaaactaga caccgcagcc accccaatag ctaagacttc ttccttagga ctcatttgag
    14041 ggtgatgaag agcgtaggga ctatcgatac ctagccaaaa catcacgcca tcaacctttg
    14101 aaaggagata accaaagtcc tcgcctgtca tagcaggttc gatatcaatc aactcgattc
    14161 cgtctttttc gtcaaagaag tccatcagtt cacgcgccaa ggctggattg ttctcaacag
    14221 gtaggtatcc accttgtttg agttccactt cgacttccat atcaaaggca gctgcaaccc
    14281 cttctgcaac tgtttttacc ctcttttgca ccaagagact catgtcctgt gtcaaggcac
    14341 gaatagttcc atgtaaaaaa gctgtgtctg tgatgacatt gttggtggtt ccagcttgaa
    14401 aaacgccgaa ggtcaccact gctccctcga ttgggttgac attgcggcta acaactgact
    14461 gcacttgggt cacaaagtaa ctagccgcca ccaaggcgtc attggcttca tgaggaaaag
    14521 ccgcgtggcc acctttacct ttgaaacgga tcttcacctc gcaagttcct gcaaagagtg
    14581 tatgagtatt agtcgcaatc tggccgactt tcaaatctgg acggacatgg aggccataaa
    14641 attgatctgg caaccaatct ccaaaagcac cagcctcata catgagcata ccaccagctt
    14701 cattttcttc agcaggctga aatagaaaga gcagattatt tttaggttgc tcctcaaggg
    14761 cgcgctcaag acagcctaag gcaatggtca tatgaaaatc atggccacag gcatgcatgc
    14821 gaccttggtg ttgagaagca aaaggtagac ctgtttgttc gacgataggc aggccatcaa
    14881 tatctgtccg ccaaccaatg gttcgctccg gctgacttcc ctgcaggtag accaaaatcc
    14941 ctgtccgcca agtacgaatt tgaacaaaat ccttgcccgt agtcaatttc tcaatcacat
    15001 ccagcaaata agcctgagtc ttgaactcct ccaagccaat ctctggaatc tggtgtaaat
    15061 ctcgtctagt ctgaatcaaa tctaacatct atctgtcctc cgatatagca gaaagaggct
    15121 ggaaaaaggg ttccgcctct tttttacttt tacaattaca aggtacgaag cgcatcctct
    15181 agcgctgttt tttgttgagt ttgggcatca atttctttga taatacgagc tggaacacct
    15241 gctactacca cgttttctgg gacatcttgg gtaacaatag ctcctgctgc gacaactgaa
    15301 ccactaccga tttggactcc ttcgataacc actgcattag ccccgatgag aacattgtct
    15361 ccgacacgga ctggttcagc actagctggc tcaatcacac ctgccaaaac tgcacctgca
    15421 ccaacgtggc tattttttcc aacgatggca cggccaccaa ggatggcacc catgtcaatc
    15481 atggttccag caccgatttc agcaccgata ttgataacag ctcccatcat gataacaaca
    15541 ttgtcaccaa tttccacctg gtcacggata atagcacctg gctcgatacg agcgttgata
    15601 gcacgcttat ctagcaaagg aactgcagaa ttacgagcat cttgctcgac aacataatct
    15661 tgattttcta ctaaaccttc aagaagcgga gccacgtcct tccaatcccc gaataggaca
    15721 ttccctagtt tgacaacaga gctaggcaca gcagttgcga gttgcccctc aaaggttact
    15781 ttgacactgg ttttcttttc agcattggcg ataaattgga taatttcttg agcgttcatt
    15841 tttgtagcag tcataggtgc ctcctggttc gttataatga tacctattct accaaaaaag
    15901 gcctcaaatt tgaaaccttt ttgtctgttt ttaggtatga ttttttaggg atgtgagata
    15961 aaacgtgctg tcggtaaaag caagccccta ccgatacaac ctaaaaacac tcttcacaac
    16021 tatctccccg tgttttattg actattctag tatagcacac ttttagaatt aagaaaagac
    16081 tttttatcta ctttctttcg cttcttctat agacaaaaat atcatgggta aaataatcaa
    16141 ggctatagct agaaggaagg accaatccac tactaatcct aagaacaaaa cactcaagag
    16201 agcagaagag agaggttcac tggcactgat aacggaaacc accaaaggag aaaccaagga
    16261 cacagccttc atggaaatga aaaaagcaaa agccgttcca aagaaagcga taatgaggca
    16321 aatcaagata ctccaaatat caagagtaaa ggaaagctga taaaccggcg agaggacatt
    16381 gctaaacaaa cctgccaaaa tcatccccca cccaaccgta ggaacaaaac cataacgctt
    16441 agcaaaaggt tggggcaaga taacattaaa cataacaccc atggcactca gcaaacctgt
    16501 tataagagct agcggcgtca tggataactg agagagatct ccctttgtcg ccatcaagca
    16561 aacacccagc atggcaacca aaacatagaa aacagcgctt tttgacgctc gtttttgata
    16621 aaccaagcga ttgtaaaaga ggataaagac agggctaata aactgtaaaa tagttgctgt
    16681 cgtagcattt gagtattcta cacagagata gaaaaaatac tgaactgaaa aaatccccaa
    16741 aatagcatag gctaaaaagg gcaggtaatt tttcttgtct cgccaaatat ctagcacttg
    16801 cgattttaat tgtattgcag accaaataag tacaagactc cctgccagtg tcaaacgcat
    16861 agaggtaatc cagcccgaag acacctgata atgagtaaag aagtactctc ctaaaattcc
    16921 acagattccc catattaagc cggataggag cgaataaatt tttccgttaa caatcttttt
    16981 ctgatactga ttcataccct tattgtaaca cgaaaggaaa aaagtggcaa tccattgatt
    17041 ggccacttca tttagagaat tatcgtctcg cccttgaaga agaaggtcgt gtagtacttg
    17101 agttactgct atcgctagaa ctactacttg aactgctgga gctggatgga gttggtagac
    17161 tccccacaat actagaccaa gcattctgat aatccgcatc acttccgcca atagcaaagc
    17221 gataacttgt cgctggcgct cctgacttat tagcccaata gctggtaaca gtcgaacctg
    17281 tgacctctac ctcttttcct tcaacagaaa ccttccctgg tttttgacct gttgatttca
    17341 agacttccga tttcactaca ctaggatcta aagcaaagcg ctcgttcccc caaatgcttg
    17401 gggaagcttg ctgaatcgca tttaccagat gagccatgta attagagtta ttagaataac
    17461 ctgctctacg tgacaatgaa tgattatcat catgcccaat ccagccacct agggttaatc
    17521 taggtgtcga aagcatgagc cacatatttt cgtcttggtt ggttgtacca gtcttcccaa
    17581 tccaatctgc attagccaga gtaggattta aagaagtcag gttagacttg aaggttgttg
    17641 tcacacgaga agatagaact tctcgtagca atccctgcat aatcgtcgca gtagcttttg
    17701 aatagacttg aaccggttta tcctgatact catacaccac tctaccatct gctgcttcaa
    17761 tctttgaaat cacatgcttc tgatgataaa ctccattatt agctaaggtc tgatagccat
    17821 tggtatgctg ggcaactgtg acttcaatac caccacccat tggcaagctc tcaataccgt
    17881 actcaggaat ctcgtaaccc atcttttcca tataaccctt gacatcaaca cccttttcac
    17941 ggagcatacg ataggtccag taagcaggga tattccatga atagttcaga gcttctccca
    18001 aggtcatcat ccctgttccc ttgctattag catacataat cggattgcca ttagcaaagt
    18061 ttgttggata gttagataga atcgtttcac ttcccatcaa gccctggtca atagcaatac
    18121 cgtaggccag caagggcttg gtagtagaag ctggcgaacg tttggtatca aaggcatgat
    18181 tattttgatt ttcttgataa ttacgaccac ctacaaagcc tagaatagca cctgtttggt
    18241 tgtccatcaa gacattccct acttctacac gacctgttcc atcgtctaaa agatagccat
    18301 aatcagcaac cgcactttgc atggcagaat gaattttctg atctatggta gtagtaatct
    18361 tataaccacc attttcaatt tccttggctg ccaaatctcg ataaaacttc tgagttgcct
    18421 catttttcaa ctccttagcg gagacattgt ctctctgagc tagataatca tacatacgtt
    18481 cttgagcttc tgccaaagtt gtaaagtata aatagtctcg tgaaattcct gtaaccgtgc
    18541 ccgatggtaa aaagtcctgt ttaaggtcat aatccttgta ctgagaatac tcgtctttgc
    18601 ttaatgcacc tgtacgatac atactgtaaa gaactgcctt agcccgtctt aagccaattt
    18661 ctaggtcttc atcactcttc aactccccag tattttcata aggagagtaa gtaatgggac
    18721 tctgtggaag tcctgctaaa aatgctgctt gaggaacagt caactgactg gcatctacac
    18781 cgaaaattcc ctcagctgct tgccgagccc ctgcaatatt ctgtccctta ttatttcggc
    18841 caaagggagc cacattgaga taggtcgtta aaatctcatc tttattcatg gcgcgttcca
    18901 aggcaagagc atccacaatc tctgccgcct tacgagccaa ggtcggcgca tccccaacca
    18961 cctgctgttt aattagttgc tgggtcaagg ttgaaccccc actagaggaa cccaaaccta
    19021 caaatttccc caaggtcgca cgaatcaccg ccttgggtac tacaccctta tgttctttaa
    19081 agtgttcatc ttctgtcgca atgatagcct tcttcagatt ttccgaaatt tgctcagatg
    19141 agatagaagt gcgcaacaaa tcactctcta tggaagcaat caccgtcccg tccgaatagg
    19201 taatctctga aatagaagag atgtccttga cctgattcac caattcttct gtctgaggca
    19261 cccgaacctt gtcaaataag gccactccgt accccaaagc aatcccaact cccaacattc
    19321 ctcctagaaa accgagtaca aagagtaagt taaataaggc ttttatactc agtaaaatag
    19381 ctgggaaaat ggctgactta tctaaggttt tagatttttt ggtacttgaa cctttcttgc
    19441 caggtctagc tgatttttta tttttttgtt tttgctggaa aaattccagc atttttcgtt
    19501 ttaattcatt taattgattt tgcatggatt tcctcactct atctattata ccacaaaagg
    19561 gaaattttca ataaaatagc cactttcttc cctattctgc taggctattg cccaagtttg
    19621 tgatacaata ggtagaaaca ataattttaa aaaggagaaa agacacatgc acatttttga
    19681 tgagctaaaa gagcgtggtt tgatatttca aacgactgat gaagaagctt tgcgtaaagc
    19741 cctagaagaa ggtcaagttt cttactatac tggctacgat ccaactgctg acagccttca
    19801 cctaggccac cttgtcgcaa tcttgacaag tcgtcgcttg caactagcag gtcacaaacc
    19861 ttatgcgctc gttggcggtg ctacaggtct catcggagat ccgtccttca aagatgctga
    19921 acgtagtctc caaacaaaag acacagtaga tggctgggtc aagtctatcc aaggacaact
    19981 ttctcgtttt cttgactttg aaaatggcga aaacaaggct gtcatggtca acaactacga
    20041 ctggtttggc agcatcagct tcattgactt cctccgtgat attggaaaat acttcacggt
    20101 caactacatg atgagtaagg aatctgttaa aaaacggatc gaaacaggaa tttcttacac
    20161 tgagttcgct taccaaatca tgcaagggta tgacttcttc gtccttaacc aagaccataa
    20221 tgtcactctt caaatcggtg gttctgacca gtggggaaat atgacagctg gtaccgaatt
    20281 gcttcgtcgt aaggcggaca agactggtca cgttatcact gttccactaa tcacagatgc
    20341 aactggtaag aaatttggta aatcagaagg aaatgccgtc tggctcaatc ccgaaaagac
    20401 ttctccatac gaaatgtacc aattctggat gaacgtgatg gacgctgacg ctgttcgctt
    20461 cttgaaaatc tttactttct tgtcacttga tgagattgaa gatattcgca aacaatttga
    20521 agcagcgcca cacgaacgct tggctcaaaa agtcttggct cgtgaagttg ttacacttgt
    20581 tcacggagaa gaatcctaca aagaagcact taacatcact gagcaactct ttgcaggaaa
    20641 catcaaaaac ctttctgtca aagagctcaa acaaggactt cgtggtgtgc caaactacca
    20701 agtacaggca gacgaacaca acaatatcgt ggaactgctc gtctcatctg gtatagttaa
    20761 ctcaaaacgc caagcccgtg aagacgtcca aaacggagcc atctacgtaa acggcgaccg
    20821 catccaagac cttgactatg tcttgagtga cgctgataag ttagaaaatg aactgactgt
    20881 tatccgtcgt gggaagaaaa aatactttgt attgacttac taaactattc aacatttatc
    20941 tataaatcaa aaccaccagt gtgaactggt ggtttgttct gcggctataa gccgcttcta
    21001 ccggccaggg ccaaaggccc accgaaatag cttcctcgcg caccactttc ccgagcaggt
    21061 gctaaagcac cttagttact tcctcttatt tatttcgcca gtaaacggat ctactgactc
    21121 gaataacgtg agctggtctg ctactctgtc ttcttgtaat tgattctgaa tatattcagc
    21181 tatcactttc tgattacggc ctaccgtatc tacataatag cctctacacc aaaacttgcg
    21241 attgccatat ttgtatttta aattcgcatg cttatcaaaa atcatcaaac tgctcttgcc
    21301 ctttaaatag cccataaagg acgaaacact aagtttcgga ggaatactga taagcatgtg
    21361 aatatggtct gaacaagcat tcgcttcatg gattattaca cccttacgct cacataagtc
    21421 acgtatgatt cttccgatac tagctttgta tctgccataa atgatttgac gacgatattt
    21481 gggtgcaaaa acaatatgat atttacaatt ccatgtggta tgtgataaac tttgattatc
    21541 ctctctcatg aggtacctcc tgtatgatat gttgtagtgg cggagaaacc acttctatct
    21601 tatcatttta ggaggttctt tttgttacca cgctaaaagc tctatggaac cactagcata
    21661 gctagtggtt ttcgggagac aacaacaaag gagttaacct cgagaaaggt aactcctttt
    21721 tgctgttaat aactctcatc tatctatttt taatagacag gctacgcaag acaatgcgca
    21781 aggttgttag attatgtaag atagagagat ttgaaggact gagccaatta aataagccaa
    21841 agccaatcaa actactattt acgacaacgg tatcttgaat atttttcttg atgagtgttt
    21901 gcaaagatga tgataacgaa tccaactctt ggaagaaatc caaacgatta tctaacaata
    21961 agatatcact catctgctta gaaatatctg cactctcatt catcaccaca ccgatatctg
    22021 atagagttaa agccgctgag tcattcaatc catctccaac catcaaaata gtgtgacctg
    22081 ctttctgcag tttctctact aactcaaatt tcccatcagg tttcaagtct gtatagacct
    22141 gatcaaaggg caaatctttg actaattcct ctgtcctaat caaggtgtct cctgttgcca
    22201 gaatcaattt tttcccctgt gccttaagtt tatccaaggc tgtttttgct tcttttctca
    22261 aaggagtatg aatgcagaac attccaatca attcattttg ataagccaag aataagagat
    22321 tgtagtgact cttgtactct tcaattaaag cattttgttc tgaactgata tgaatctgct
    22381 catcctgcat caagacataa ttcccaataa gaactggttg gccatctata tgagatttga
    22441 tccccttgct tgcgatatat tggagtttcc catgcatttc ctcatgttca attccctcta
    22501 tctcagcttg cttgacgatg gcattagcaa taggatgata aatgtgttcc tcaagacagg
    22561 cactgattct gagaatatct tcctcactat agtctccaaa aggtaacacc ttttcaacta
    22621 taggataact agttgtgatt gttcctgtct tatcaaacaa gaaagtatca acttccagat
    22681 atttctccag aacatctcca tccttaatca ccatttcacg gttcaaccct tccttgataa
    22741 ctgtcaaata agctacagga gtagagattt tcaaagcgca ggagaaatcg accaatagga
    22801 aagaaatagc cttagaaaaa gaacctgtca ataggtaagt cagcccagcc cccaagaaat
    22861 tatatttaac gactttatcc gccatcttga tgaaatagcg ttgtttcgtt ttcttgtttt
    22921 cttcagattt cttcatcaac tcaatcagct gtaaaatacg gctgttcatc tgattatctg
    22981 ttacacgaat gcgtaactct ccagtttcta atactgtatt tgcacaaacc aaatcagact
    23041 ctcttttttc aactggaaaa ctctctcctg tcaaggaact ttcgttgacc atacctaaac
    23101 ctgaaactac ttgtccatca aacagaattt catttccttg agataagatc aagacatctc
    23161 ctatttgaac atcggaactc ttgatactaa caaccgtatc gccctgtact aggaatacat
    23221 cgctctcttt tgcaagaaga ctctgttcta aatctgttgc agtttttttc aaggaccact
    23281 gatctaaatg attccccaaa tcaagcataa acatgatatt gctagctgtc ttggattggt
    23341 tcataaacaa agacaataaa atagccgaac agtccaagac ttccatcgtt agttccttac
    23401 gcgctagtgt ttgataggct tctctaatat aacccaaagc ctgataacaa gtccatatat
    23461 agcgaatagg atacggcaca aaaccacgaa aaagtacacg cttaaccgct gcacctgaaa
    23521 caatagaata agcactctct tctctacgaa tgggaagagt catcaactca gaaactttcc
    23581 ctttatcaat tctttttaaa aaggcttctg cattatctaa tacagaaaag ccttctttta
    23641 tgcgtagagt aaagtgctgt tgatccatgt aaaactggat agactcaatc cccttttcat
    23701 ctctcgccaa ggaacgaaga tagtcttgaa tatccaaggt aagtgaaaaa gaagatgata
    23761 gtcggatatg ttggtatcct ctatgtagca ctttaaaaga catattattc acctataagg
    23821 ctatctaatt gctcttcttt tttctcttgc tcgtacaaat atttggcatc ttgcaagaca
    23881 tcgtctccat gttgcttcac aacagaaaca gatgcatcta gctcgtcttt caacttgtaa
    23941 gccttagcca aagctttaga ataacctttt ttagcttcct tacttgctaa gattttcaaa
    24001 ccaagggtac caaatgcgac accacccaaa aataatgaag attttttcgc aacttttgca
    24061 acggttaata cttcttttaa catacttatt tcctccttat cattattata tagcaattta
    24121 gatataaaag caatttcatt ctataaattg gagaattagt tttatttcta ctgaatctcc
    24181 catctactta ttcctaaagt tgctttcatt tgcctctttt gatacactta aactatgaat
    24241 acaaatctca agcccaaact tcagcgtttt gcttctgcga ctgcctttgc ctgtcctatc
    24301 tgtcaagaaa atctgactct gttagagact aatttcaagt gctgcaaccg tcattctttt
    24361 gacttggcga aatttggcta tgtcaatcta gcacctcaaa tcaagcaatc tgctaactat
    24421 gacaaggaaa attttcaaaa ccgtcaacaa atcctagaag ccggctttta ccaagctatc
    24481 ttagatgctg tatctgactt gcttgcaagc tcaaaaacta ccacaacaat tttggatatc
    24541 ggttgtggtg aaggattcta ttctcgcaaa ctacaagaaa gtcactctga aaaaactttc
    24601 tatgcctttg acatctccaa agattcagtc caaatcgctg ctaaaagtga acccaactgg
    24661 gcagtcaatt ggttcgttgg cgacttggca cgacttccta taaaagacgc taacatggat
    24721 attctgcttg atatcttctc acctgccaac tatggagaat ttcgtcgcgt tttatccaaa
    24781 gacggtatct tgataaaggt tatcccaact gaaaatcacc tcaaagaaat ccgtcaaaga
    24841 gtacaggacc agctgacaaa caaggagtat tctaaccaag atatcaagga gcatttccag
    24901 gaacacttta ccatcctatc tagtcaaact gcctctctga ctaagactat cacagcagaa
    24961 caactccaag ccctactcag tatgactcct ctcctctttc acgttgacca gagcaagatt
    25021 gactggagcc aactgacaga gattaccatc gaagcagaga ttctggttgg gaaagcattc
    25081 taaactagac ttcccaaagg tataaaactt accgaaaatc ttcaatatta caaaaacgca
    25141 taagaacagg tacacaaacc ttaatcttat gcgttttgtt atagtttcca tacacattaa
    25201 ggggaaatag ttgaaatcag ttctcttaat aaccattgaa taccatatac tacatttacc
    25261 ccaaatatga tataggccgt ataaatcaag tatttcgatt tctcccgtct caaagaatag
    25321 atcttgtaca aaatagataa atttaaaata ataaataata gagacaaaat cttcctccaa
    25381 aatcaatatt aaaagtagcc agtcaatcat agttctaatc tttattttcg attcgaaatt
    25441 acaaacttat caatgaacaa taagtgatat aaggccagta ccagacccca aacgctacca
    25501 cgaaaagaga attcttgatg ggtaaggggg aaaagactac tattacccgt caagaggcat
    25561 gccccaatta caaaaagaat ccaaaataaa tccgtccatt tatattttga aaaatatttt
    25621 tttaacataa tagcatattt ttcttgacta taataaaagt atataataaa atgtaagcag
    25681 atacaaataa aaaggagaaa agctcatgaa caaattaatg aaatttattt cggttttttt
    25741 gacgtcaatt gtgttaattg tatcagcgat tccaagtgtt tcagctgtat acgcttctga
    25801 acaagtatca caaattgaaa caaatatgga acttcaacct gtcacttctc taacagaaga
    25861 acaaatcaat acacttgcaa acgaaatcca atcttttcat ccagacgtct cacaacaatg
    25921 gatcaaagaa gtaattaacc gacaattaca aggcgattat acaatcccac ctacatactc
    25981 tccatttaga gcagcttggc aaggtattac agttaatcaa atgggtgctc tattagatac
    26041 tgcaatagct ttagcattag gaggaactac tgcaggcctt gcaaatctaa ttaaagtaaa
    26101 aggaaaacat gcagcaaaaa gtgctattcg ttcagcaatt tctagatatc taggtagttg
    26161 gtttgtaaat gatgttgctt tagcattcgc tatgaattta ttatcaccgg ggacttattt
    26221 agcacaatta tgggataaaa atgatgccat tcctaacaac ggaaggatta acttttaatc
    26281 atgagatata caataaaatt aattgggtat aacgtatttt cgttgtttat attctttatc
    26341 ttatcaatgt tgtttaaagt tccctttagg tggtggatca gcattgcaat gacgacttcg
    26401 tcgtacattg tatataatta cttaaagaaa aagagagaaa aggaatgatc ttataccaga
    26461 tcattccttt tctgctttct gtaaatccct cggagtgtgt ttggcgcttt gatacgaagt
    26521 gtcaaagctg tataaacgtt actatttcca aaaaagtaac agaaaattcc tgctttgtag
    26581 gactgctttg ggtgagtaat agacatgtcg actcacccta tctcctgatg gcgaaaagaa
    26641 tttagcaatg atgcaagttg ttctgaactc gagaaaaaac atgccgaaat cgaagaaaag
    26701 cgtgcagaac ttgaagcgaa gaagcaagaa atgttgaagc gcttggataa atagaattta
    26761 gaaaacagaa aaaactaaaa agaatgtagg gtttatttcc ctacattctt aaaataactt
    26821 agtacataaa tgacaatgct gccactgccg tcaatattgc aaaaactatt ttccaaattt
    26881 ttttattttt tccaaaaatc ttatcaggta acaagcttat tacaagaaag gtaataaaca
    26941 acaccaattt aagtgtgtat atcataaaaa caccttcttt cctacaatta tattataata
    27001 ctttatgtaa gcgaatacaa gagatatgag gtaaccaact acgaaaaaat ataaggctga
    27061 agacagtaaa aaccagcatt taaagccttc actgtatcta ggatttccct atttttagac
    27121 aagattgacc caaatctaca tcattaaaaa agcgaatttc aatcgaaact cgctttttta
    27181 caagatgtat tagtataaat ttgtatctta tactcaatga aaatcaaaga gcaaactagg
    27241 aagctagccg caggttgctc aaagcaccgc tttgaggttg cagataaagc tgacgtggtt
    27301 tgaagagatt ttcgaagagt attagttcaa gtgccagatg tcttcgttat actgagcgat
    27361 tgtacggtca gatgagaaga atcctgcttt agaaatgtta acgatgactt tatccaacca
    27421 tgcgtcacgg tcttcgtagt cagcaagcat ttgctctttg actttgatgt agtcttccaa
    27481 gtcaagaaga gtcatgaacc agtctttgtt gatcaattca ttgtaaagac gttccaagcg
    27541 ctctttgttt ccagctgcaa gaactgcatc actaacgatg aagtcaacca atggtttgat
    27601 agcttcacga gcgtagaatt cgcttgattt gtaagctgct tttgcgtaaa ggtcgataac
    27661 agtttctgaa tcttcaccga agatgtagat gttttcttct ccaaccaact cagcgatttc
    27721 cacgttagca ccgtccatag taccaagtgt caaagctccg ttcaacatga atttcatgtt
    27781 accagtacct gaagcttctt tagaagcaag tgagatttgt tctgagatat cacatgctgg
    27841 gataaggaag cttgctgcag taacgttgta gttttcaacc ataactactt gcaagtgtgg
    27901 agctactgct ggatcgttag caataacttc tgacatgcaa aggatcaagt gaatgatatc
    27961 ttgagcgatt gtgtaggctg gagctgcttt accaccaaag aagattgtga ttggacgagc
    28021 agggatgtta ccagctttga tgtcaaggta tttgtggatc acgtacaaag cgttcatttg
    28081 ttggcgtttg tactcgtgaa gacgtttgat ttggatatca aagatagaat ttggattgat
    28141 ttccacacct tggtgttctt tcaagtgacg agccaattta cgtttgttgt gagccttgat
    28201 gctttccaat ttttctttga cagctgcttt gtcttcataa gacaacagtt tttcaagctc
    28261 atctgcttca tggtgccaac catctccaag aatctcatcc aagtagtgag acaatcttgg
    28321 gttagcatgc ataagccaac gacggaaagt gataccgttt gttttgttgt tgaacttttc
    28381 tgggtaaagg tcgtagaagg ctttcaactc agaatttttc aagatttcag tatggagtgc
    28441 tgcaaccccg ttaacactgt atccgtagtg gatatccatg tgagccatgt gaacacgtcc
    28501 gctctcatcg atgatttgaa cagctggatc tttgtactct gccttcacac gacggtccaa
    28561 ttcttcgatg attggtacca agtgaggaac cacttcttgc aagaattcaa gaggccattt
    28621 ttcaagcgct tcagcaagga ttgtgtggtt agtgtaggca gtcatgctac gaacaattga
    28681 gattgcttcg tcaagatcga taccacgtgc agtcaaaaga cgaatcaatt caggaatcac
    28741 cattgatggg tgagtatcgt tgatttggac aactgcgtag tcagcaaggt catgcaagtt
    28801 gcttcctttt tcgattgctt cgtcgatgat caattgcgca ccgtttgaaa ccatgaagta
    28861 ttgttggaag atacggagca attcaccttg acggtcacta tcatctgggt aaaggaagag
    28921 agttaagttg cgagcgatat ctgtcttgtc aaagttgata ccatctttaa taatagaaga
    28981 atcaactgaa tccaagtcaa acaaacgcaa gcggttttta gtcgctgttt cataaccagt
    29041 aacatcaata tcgtaaagag ttgatgtcaa agtaaagtct gcaaatggta cttggtagct
    29101 acggcttgag cgaaccaacc agttttgctc tgtcaaccat gcatttggaa ttgtttcttg
    29161 ttggttgttt ttaagaactt gttggaaaag accaaagtgg tagttaagac caacaccgtc
    29221 accattcaaa ccaagagtag caattgagtc gataaagcag gcagccaaac gtcccaaacc
    29281 accattacca agagatggtt ccaattcaac ttcttcaact tcgatcaagt ctttacctgc
    29341 agctgcaagt tcttttttaa catcgtcgta aagaccaagg ttaatcaagt tgtttgacaa
    29401 gagtttacca atcaagaact cagctgagat gtagtaaact tttttcttac cagtgttgac
    29461 tggtttttgg ctgcttgcaa gcttgctgta gttaagaaga gcaaggtaaa gctcttcatt
    29521 gctacattct gcaatggttt tattgtaacg attttgtaca aattcttgta gtgataacat
    29581 gtttagttgt ctcctgatta ttgtcttatt tctttaaatc taccaaattt tcattaattc
    29641 ggcgataaat tgttgtcaag tcaagcaaac cttcctcgac agctggtgtc aattgatctt
    29701 cagtcatacg ccaagaccag tttccaccaa gggtagatgg gaagttcata cgagctgcct
    29761 catccaattc tagtaaatct tgcatagttg caattgccat aaagctaact gatgaaaata
    29821 ctgtacgaag catagcgtgt accactgttt cgtattcttt acggttcgtg taacgagcca
    29881 tgtactcacg agtcgcatca tcaatctcat tacggtacca accaagaacc gtattgttat
    29941 cgtgtgttcc tgtgtacata actgagttag caggtgccaa gtgtgggcta tcaatgcttt
    30001 cgtcttctgg gttgaaggca aattgaagaa tcttcattcc tgggaagcca gtacgttcac
    30061 gcaattcgat cacttcatct gtcatgaagc caaggtcttc tgcgatgatg tttagctcac
    30121 caagttcttc cttaacggct gcaaaaagct tgtagcctgg acctttcacc cactcaccag
    30181 gtgctgctgt atcggaacca gcagggattt gccagtaaga ttcgaagcca cggaagtggt
    30241 cgatacgaac gatatcgtag attttgaagc tttcacgcaa gcgttcaatc caccatttgt
    30301 aaccgtcttt gtccattgct tcccagtcat agattgggtt accccaaagc tgaccagtta
    30361 cagaaaactc atctggtggg catcctgcga tacaagtagc cttaccattg acatctgttt
    30421 tgaagagatg tggatttgcc cacatatcac ttgaatcttc cgctacgtag attggcatgt
    30481 ccccaacgat ttcgatgtgg ttgtcgttag cgtaagcttt caatttcaac cattgttgga
    30541 agaagaagta ttgagtcaca cggtggtaaa ccaacttgtc tgccaattgc tcacgatagc
    30601 tttcaagtgc tgaagcttta cgagcacgag catctgcatc tggccattca gtccaagcaa
    30661 gattgtcaaa atactctttg atagccatat actcagcaaa gagctcaagc catgattggt
    30721 tgtcttgagc aaatttctca aaatctttaa catctccgac ttcaaagaaa cgtttcaccg
    30781 ctttttctaa aagaggacga cgtgcatagt agattttagc atagtcaact tcagacgcat
    30841 cgctaccaaa gtcaactcct tcaaggtcac ttgcttccaa caaaccttgc tccaccaaga
    30901 tatctaaatc gataaaatga gtgtttcctg cgaaggctga gaaagattgg taaggagaat
    30961 ccccgtaact agttgctcct aatggaagga tttgccagta acgttgtttt gtacggacca
    31021 agaaatcaac gaagtcgtaa gcactttgac caaatgatcc gattccgtaa gctcctggaa
    31081 gagaagagat gtgcatcaac acaccacttt gacgtttttt cataatagca cctcgtgtgt
    31141 taaaataatg gaacgttgcg tattttgcag acgcaaacgt ttgcgttact tataagtata
    31201 ctccttttca agcataattg caagcgtttt taaaaaactt ttttgattat ttttaaggag
    31261 actaactcat tttagcaaag ataacttaaa actatctaat cctaaagttt gactctcacg
    31321 agacagtcta caaagtacaa aacctcctta gggagcttga atttatatgc agcaaagcac
    31381 aaacgtctat agaaaataga tagggattag aaacagggag gtagcccctc ctgatttccc
    31441 tctttagaca gattccatat attttttatt aaaaatactg tattcttatc tatttcatac
    31501 ttttcttcta ttaaaatgca aaattatgct atcaaatcaa gcttaaaaat tttttaaaat
    31561 ttttacaaaa aatacttgca accgttttct atttgtgcta tactaagctc ataaaggaaa
    31621 acgtttgcgt ttccttatca ataaaacttg ctattctttg ggaggaatac actatgtcat
    31681 ctaaatttat gaagagcact gcggtgcttg gaactgttac acttgctagc ttgcttttgg
    31741 tagcttgcgg aagcaaaact gctgataagc ctgctgattc tggttcatct gaagtcaaag
    31801 aactcactgt atatgtagac gagggatata agagctatat tgaagaggtt gctaaagctt
    31861 atgaaaaaga agctggagta aaagtcactc ttaaaactgg tgatgctcta ggaggtcttg
    31921 ataaactttc tcttgacaac caatctggta atgtccctga tgttatgatg gctccatacg
    31981 accgtgtagg tagccttggt tctgacggac aactttcaga agtgaaattg agcgatggtg
    32041 ctaaaacaga cgacacaact aaatctcttg taacagctgc taatggtaaa gtttacggtg
    32101 ctcctgccgt tatcgagtca cttgttatgt actacaacaa agacttggtg aaagatgctc
    32161 caaaaacatt tgctgacttg gaaaaccttg ctaaagatag caaatacgca ttcgctggtg
    32221 aagatggtaa aactactgcc ttcctagctg actggacaaa cttctactat acatatggac
    32281 ttcttgccgg taacggtgct tacgtctttg gccaaaacgg taaagacgct aaagacatcg
    32341 gtcttgcaaa cgacggttct atcgtaggta tcaattacgc taaatcttgg tacgaaaaat
    32401 ggcctaaagg tatgcaagat acagaaggtg ctggaaactt aatccaaact caattccaag
    32461 aaggtaaaac agctgctatc atcgacggac cttggaaagc tcaagccttt aaagatgcta
    32521 aagtaaacta cggagttgca actatcccaa ctcttccaaa tggaaaagaa tatgctgcat
    32581 tcggtggtgg taaagcttgg gtcattcctc aagccgttaa gaaccttgaa gcttctcaaa
    32641 aatttgtaga cttccttgtt gcaactgaac aacaaaaagt attatatgat aagactaacg
    32701 aaatcccagc taatactgag gctcgttcat acgctgaagg taaaaacgat gagttgacaa
    32761 cagctgttat caaacagttc aagaacactc aaccactgcc aaacatctct caaatgtctg
    32821 cagtttggga tccagcgaaa aatatgctct ttgatgctgt aagtggtcaa aaagatgcta
    32881 aaacagctgc taacgatgct gtaacattga tcaaagaaac aatcaaacaa aaatttggtg
    32941 aataaaaaat ttgttcaagg ggggtggaaa tcaaatcccc ctttgaattt atcaatagag
    33001 acacaaataa tttagctttc ttataaaaaa gtagtatcct atgaaaggag ttaatatgga
    33061 aaagcaacaa cctagtaaag cagccctgct gtctatcatt cctgggttag gacaaattta
    33121 caataaacaa aaagccaaag gttttatctt ccttggtgta accatcgtat ttgtccttta
    33181 cttcctagca cttgcaaccc ctgaattgag caacctcatc actcttggtg acaaaccagg
    33241 tcgtgataat tccctcttta tgctgattcg tggtgccttc catctaatct ttgtaatcgt
    33301 ttatgtactc ttttatttct caaatatcaa agatgcacat acgattgcaa aacgcattaa
    33361 caatggaatt ccagttccac gcacactcaa agacatgatc aaagggattt atgaaaatgg
    33421 cttcccttac ctcttgatca ttccatctta tgttgccatg accttcgcga ttatcttccc
    33481 agttatcgta accttgatga tcgcctttac caactacgac ttccaacact tgccaccaaa
    33541 caagttgttg gactgggttg gtttgaccaa ctttacaaac atttggagct tgagtacctt
    33601 ccgttctgcc tttggttctg ttctttcttg gactatcatt tgggctttgg cagcttctac
    33661 tttacaaatc gtaattggta tcttcacagc tatcattgcc aaccaaccat ttatcaaagg
    33721 aaaacgtatc tttggtgtta ttttccttct tccttgggct gtcccagcct tcatcactat
    33781 cttgacattc tcaaacatgt ttaacgatag tgtcggtgct atcaacactc aagtattgcc
    33841 aatcttggct aaattccttc ctttccttga tggagctctt attccttgga aaacagaccc
    33901 aacttggact aagattgcct tgattatgat gcaaggttgg ctcggattcc catacatcta
    33961 cgttttgact ttgggtatct tgcaatctat tcctaacgac ctttacgaag cagcttatat
    34021 tgacggtgcc aacgcttggc aaaaattccg caacatcact ttcccaatga ttttggctgt
    34081 tgcggcacct actttgatta gccaatacac cttcaacttt aacaacttct ctatcatgta
    34141 cctcttcaat ggtggaggac ctggtagtgt cggaggtgga gctggttcaa ccgatatctt
    34201 gatctcatgg atctaccgtt tgacaacagg tacatctcct caatactcaa tggcggcagc
    34261 tgttaccttg attatctcta tcattgtcat ctcaatctct atgatcgcat tcaagaaact
    34321 acacgcattt gatatggagg acgtctaaga tgaataactc aattaaactc aaacgtagac
    34381 tgactcaaag ccttacttac ctttacctga ttggtctatc aattgtaatt atctatccac
    34441 tgttgattac cattatgtca gcctttaaag caggtaatgt ctcagccttt aaactagata
    34501 ctaatatcga cctcaatttt gataacttta aaggcctctt cactgaaacc ttgtacggta
    34561 cttggtacct caacactttg attatcgcct taattaccat ggctgttcaa acaagtatca
    34621 tcgtacttgc tggttatgct tacagccgtt acaacttctt ggctcgtaaa caaagtttgg
    34681 tcttcttctt gatcatccaa atggtgccaa ctatggccgc tttgacagcc ttcttcgtta
    34741 tggcgcttat gttgaacgcc cttaaccaca actggttcct catcttcctc tacgttggtg
    34801 gtggtatccc gatgaatgct tggctcatga aaggctactt cgatacagtg ccaatgtctt
    34861 tagacgaatc tgcaaaacta gacggtgcag gacacttccg ccgcttctgg caaattgttc
    34921 ttccacttgt tcgcccaatg gttgccgtac aagctctctg ggccttcatg ggacctttcg
    34981 gggactacat cctctctagt ttcttgcttc gtgagaaaga atactttact gttgccgtag
    35041 gtctccaaac cttcgttaac aatgcgaaaa acttgaagat tgcctacttc tcagcaggtg
    35101 ctatcctcat cgcccttcca atctgtattc tcttcttctt cctacaaaag aactttgttt
    35161 caggacttac aagtggtggc gacaagggat aatttatccc cgccaccctt tttcatttta
    35221 tactcttcga aaatctcttc aaaccacgtc agctttatct ccaacctcaa agttgtgctt
    35281 tgagcaacct gtggctagtt tgcactttga ttttcattga ctattagcaa ttgtcactgt
    35341 aaataatatc cttgtagcaa gcaatttttc tcctagactt gaaataaagc gcatttctct
    35401 atataataat acttatatag aaaacacctt ttagaaagat acctatgctt ccatatccat
    35461 tttccttttt ttcaagtatt ttggggtttc gtaagcccct gtccaaacgt ttcgggctca
    35521 actggtttca acttctcttt accagtatct tccttatcag cttgtctatg gtacccattg
    35581 ctatccaaaa cagctcccag gagacctatc cgctagaaac ttttatcgat aatgtctatg
    35641 aacctctgac aaatgaggtt gtccaggatc tttctgaaca tgctacaatt gtcgatggca
    35701 cattaactta tactggaaca gttagtcaag cctcttctgt tgtgattggt ccaagtcaaa
    35761 tcaaggaatt acctaagaac ttgcaactgc atttcgatac aaatgagcta gtcatcagca
    35821 aggaaagcaa ggaactgacc cgcatctctt accgagccat tcagactgag agtttcaaaa
    35881 gcaaagacag cttgacccaa gcaatttcta aagactggta ccaacaaaat cgtgtctata
    35941 tcagcctctt cctagttctc ggtgcgagct tcctctttgg tttgaatttc tttatcgtct
    36001 ctctaggagc tagtcttctc ctttatatca ctaagaaatc acgcctcttt tcatttagaa
    36061 cctttaaaga gtgctatcac tttatcttga actgtttagg attgccgact ctgattacac
    36121 ttattttggg attatttggc caaaatatga caaccctgat tactgtacaa aatattcttt
    36181 ttgttctgta tctggtcact atcttttata aaacacattt ccgtgatcca aattaccata
    36241 aataggagat ttttatgccc gttacgatta aagacgtggc caaggctgct ggtgtttcgc
    36301 cttcaaccgt aacccgtgtt attcaaaata aatcaaccat tagcgacgaa acaaaaaaac
    36361 gcgttcgcaa agctatgaag gaactcaact accacccaaa cctcaacgct cgtagcttgg
    36421 taagcagcta tactcaggtt atcggattag ttcttcctga tgactcagac gccttctacc
    36481 agaatccttt ctttccatcg gttctacgtg gcatctctca agtcgcatct gaaaaccact
    36541 atgccattca gatagcaaca gggaaagatg agaaggagcg tctcaacgct atttcacaaa
    36601 tggtctacgg caagcgtgta gatgggctaa tctttctcta tgcccaagaa gaagaccctc
    36661 tcgtaaaact cgtcgcagaa gaacagttcc ccttccttat cttaggtaaa tctctatctc
    36721 ctttcatccc acttgtcgac aacgacaatg ttcaagctgg ttttgatgcg actgaatatt
    36781 tcatcaaaaa aggctgcaaa cgcattgcct ttatcggagg aagtaaaaag ctcttcgtga
    36841 ccaaagaccg tttaacgggc tatgaacagg cgcttaaaca ttacaaactt accactgaca
    36901 acaatcgcat ctactttgcc gacgagtttc tggaagaaaa gggctataaa tttagcaagc
    36961 gattattcaa gcacgatcca caaattgatg ctatcatcac aaccgatagc ctcctagctg
    37021 aaggtgtttg taactatatt gccaaacacc agctggatgt ccctgttctc agctttgact
    37081 cggttaatcc caagctcaac ttggcagcct atgtcgatat caatagttta gagcttggtc
    37141 gtgtttccct tgaaactatt ctccagatta ttaatgataa taaaaacaat aaacaaattt
    37201 gttaccgtca attgatcgcc cacaaaatta tcgaaaaata agagactggg caaaaagtcg
    37261 ttaaaagcaa aaacgcatac tatcaggtat tgaaaaaact tgatagtatg cgttttattg
    37321 tgggaagatt tacttccttt tcttctgaaa ttgagttgtt acccaagatc tttttatact
    37381 caataaaaat caaagtgcaa actaggaagc tagccgcagg ttgctcaaaa cactgttttg
    37441 aggttgtgga tagaactgac gaagtcagct caaaacatgg ttttgaggtt gcagatagaa
    37501 ctgacgaagt cagtaacata cctacggcaa ggcgacgctg acgtggtttg aagagatttt
    37561 cgaagagtat taatcactaa ttatctatct caacaaatct tcctagaata tgaacatttt
    37621 ccgagacaga gacaaaggag cttggatcca cttgtgtcat aatctgttta aattcattaa
    37681 actctgcacg tgtaatgaca gtgattaaaa ctgcctttct ctcgtgatta taggttcctt
    37741 ctgcatcgtg gatcatggtt gctccgcggt gcaatttttt atggattttt tcaattacct
    37801 tctctggatg atttgtcaca atcatggcct gcatacgctt ttgcttagta aagactgcgt
    37861 ctgtcacacg gctagagaca aagatggtaa tcatagaata aagagcgtat ttccaaccaa
    37921 aggtcaaacc tgctatcagc atgatagttc catttaccaa gaaagaaata ctaccgacat
    37981 tcttacccgt tttcttgcga atagtcaggc tgacgatatc cgtcccacca ctggagatat
    38041 tgtttcgaag agcaaaacca atccccaaac ccataacaac acccccaaaa agggaattga
    38101 taatgggatc ctccgtcaag gttgccacag ggacaaactg gataaagaag gaactcatag
    38161 ataccgtgat aaaggtaaag acggtgaact tatggccaat ctgataccaa gctaagacca
    38221 tcaaagggaa gttaatggcg tagaagctta gcgaaatcgg aatatgaaaa ccaaaccagt
    38281 gattactcaa ggcagagata atctgtgcca gacctgttgc accactcgaa tacacatgcc
    38341 ctggttggaa aaagaaatta actgctactg ctgataaaaa accatagacc agagaggccg
    38401 aaatcttctc atcatacttt tctcgagaga tactttgtaa gacacgtaaa atttttatct
    38461 gataagcaaa gcggcgcaga taatagcgcc accgcttaat tcgttttgtt tgtttcatct
    38521 tcttctactt gtaagctgag ttcctctagt tgtttgagag cgactgttga tggagcttgt
    38581 gtcattgggt cagttgcctt gttgttctta ggaaaggcaa tgacttcacg gatattttct
    38641 tctccagcaa gcaacatgac aaaacggtca agcccgatag ccaaaccacc gtgtggtggg
    38701 aaaccatagt ccatggcttc aagaaggaaa ccaaactggt cattggcttc ttcagctgag
    38761 aaaccaagag ccttgaacat gcgttcttga aggtcttttt ggttgatacg aaggctacca
    38821 ccaccaagct cataaccgtt caagacgata tcgtaagcaa tggcacgaac cttagccaaa
    38881 tcaccttcta attcgtgagc agtctcttcc tgtggaagtg tgaatggatg gtgggcgctc
    38941 atgtagcggc cttcttcttc agaccattca aacatcggcc agtcaaccac ccaaaggaag
    39001 ttgaacttat cattatcaat caagccaagc tctttagcaa tacgtccacg aagggcaccc
    39061 agtgttgcat tagccacttc aagcgtatcc gccacaaaga gaaccaagtc cttatcttca
    39121 agagcaagcg ctgttgtcaa ttcttcttgg ataccagtca agaacttggc aactggtccg
    39181 tttaattctc catcaaccac cttgacccaa gcaagacctt tggcaccata ctgtttggct
    39241 acttccgtca tcttgtcgat gtctttacgt gaatagttgt ccgcagctcc tttgaccaca
    39301 atcgctttta cagcaagtgc ttctgaaaag actttaaagt ctacaccttt gaccacttct
    39361 gtcaagtcct gaagcaacat gtcaaaacga gtatctggct tgtcagaacc gtaaagagcc
    39421 atagcatcat cgtatttcat acgagggaac ggtagcgtta cttcgatgcc ttttgtttcc
    39481 ttcatcacgc gcgcgatcaa gccttctgta atatcttgga tttcttgctc agtaaggaag
    39541 gacgtttcca agtcgacctg agtaaattca ggctggcggt ctccacgcaa gtcctcgtca
    39601 cggaaacatt taacgatttg gtagtaacgg tcaaaaccag cattcatcaa gagctgtttc
    39661 gtgatttgtg gactttgagg aagagcgtaa aaatgcccct tattaacacg agacggcact
    39721 aaataatcac gcgccccttc aggcgttgac ttagaaagga atggtgtctc cacgtcgata
    39781 aactccaact catccaagta gttgcggata gagtgggtca ccttggcacg aagtttaaga
    39841 ttttccaaca tttctggacg acgaaggtca aggtaacggt aacgcaaacg tgtatcgtca
    39901 tttgcctcaa tgccatcctt aatctcaaat ggtgttgtct tagctgtgtt aagcacaata
    39961 agagctgtca cgtttaactc aaccgcacca gttggcaact tatcattggc ttgctcacgc
    40021 gcagcgacct gaccagtcac ctcaataaca aattcgctac gaaggctttc agctgttgcc
    40081 ataacctctg cagatacttt ttcagggttg ataaccaact gcatgattcc ttcacggtca
    40141 cgaagatcga taaagatcaa accaccaagg tcacgacgac ggccaaccca tcctttcaag
    40201 gttatttctt gtccgatgtg ttcctcacga acacgaccag catacatact acgtttcatt
    40261 atttctctcc tcttttattc tgttactatt ttaccataaa agcgcaggct cttcatgaaa
    40321 atcatcagaa aagtttgcca gtctttaaaa gtcaggtgaa agccctaaaa attagcgcta
    40381 atgctcttcg aaaatctctt caaaccacgt cagcgtcgcc ttaccgtagt acggttactg
    40441 acttcgtcag tttcatctac aacctcaaaa ccatgttttg agctgacttc gtcagtttca
    40501 tctacaacct caaaacagtg ttttgagcaa cctgcggtta gcttcctagt ttgctctttg
    40561 attttcattg agtataatac aaaaatccga tgaacttcac cggactcttt tattttgaat
    40621 ttttgcctgc tttacgcttt tcagcgattt cggctgcctt tcgaggcaag acaatttccg
    40681 ttatgtaagc cgtcccaaaa cgcagtacac ctgcaatagg agcaaagaca actgctagat
    40741 agttatagaa gaaatcgcct ttgaaggcat aagctagcgc tccaatgatg aaaaatagaa
    40801 cgactgcctg aatcactgct aataaaatta ctcgtttcat gtgacctcct gactctatta
    40861 tagcatgaga atcatcaaaa agccgactaa attattcaaa gcgtgaagag aaatactgta
    40921 gactagacct tttctgctaa tgtaagccaa acccaaacta aaaccaaggc taaaatagac
    40981 aaaaaattgt tgcacatcac ctggaaaatg aatcaaggca aatagaagac tagataccag
    41041 aagaaaaatc agggttcgtt tactattgtc ctgcttagga aagagatagc gtgctaacat
    41101 ccctctaaaa acaatctctt ccgtcaaagg agcaaaaata accacagcaa agaatgagaa
    41161 aagtggttga gacaaggtca agtctgtcgc tatttgctga tttactgaag gatcatctgg
    41221 caagaagaat tgaacgacca gagataagaa ccaaaccaag acaggaagcc aaataaatcg
    41281 gttaaagccg ctcttctcaa tatgaacagg agccttctga taccatttgt aaatgccgta
    41341 cacatatact acagccaagg ccacatagag tagagtaaca gcatagggtg aagcgcctaa
    41401 agcaagcgac gcagtcgcga gcccctgaat aaagccatag ataaataaaa aggatagaag
    41461 ggctagaaga atccagccaa ggtttttaag taatttcata gataactcct ttatttgaaa
    41521 taacgtttta ccataggtaa ctgcatcaca ttgatataaa catggatggc tcctacaagc
    41581 aagaaagcta gtaactgaat ctctcctgtc aagaaagaaa ggataataag aaaaatatat
    41641 aaggctggta agacatattg gtgtaattgg aataaaattc gaaaactctg ttccaaatta
    41701 gcctgacgct ccccttcatc ataagaattt atatagttca agacatcctt tggtgtagcg
    41761 aaaaattcca aatcaaactg acgaacaatc gcaatggttt taaaaagaga tttttgagcg
    41821 actaagaata ccacaaagag taagaaagaa aggaaaaatg tttgagggtt tgtatgcaat
    41881 ataatcacct cacttaatga aataaaaata gccaatggaa tcgctacacc tgtaatatta
    41941 aaagcaatgg ttccaaactc aagattccga tacatttgca cataataggt ttcattcaga
    42001 tcgtcatcca tttcctcttg atacaaggaa tgaaattttc tgcttttctt taagaaattg
    42061 aaagtcaaaa acatactaat gaaacctatc agtaaacaaa tagctgatat ccatggcatc
    42121 aaggctttta catctaaaat aatttcgtgg gattcgacac gtgccttaaa catccctaca
    42181 aacatgccca agaacccccc caagacaata gacatcaaaa ataacaatct acgtttcttt
    42241 ttcatattca ttctcctttt tcacttgcta gatttttgga tttcttttca atccattcaa
    42301 ttactgggat gagagcaaag tagacccaaa caaattggtc gctttgatag ggattaaacc
    42361 agcttaggtc catcccaatc agtagaaata cgctgactaa taaagctatg accactacat
    42421 aataaatcac tttatacttg ttcatcactc gtcctcctcc aaacgaaata ccgattcgac
    42481 tgtttcgttg aaaatttgag atattttcag ggcaatgata atggatgggg tgtactcatc
    42541 ccgttctagt aggctaatgg tctgtctgga aacccctgcc agtttggcta ggtcggtttg
    42601 attgagacca tcgcgagctc gaagctcttt tagacgattt tttagttgca cgttacacac
    42661 ctactctccg tcaaattcaa cggtttggat atcttcaata cgttgcaact tgaatttttc
    42721 ttttcccgta ttatctacac gtcgtagctt tacccattcc tcatcaacat ccacaacttc
    42781 ccagttatct ggcccaatat acactcccgt tataattggt ttctttccaa tcatttcttg
    42841 caataatctc gacatttctg cgtttccttt ctcttttcgc tcaagttttt tgattttatt
    42901 ctctagtttc ttgatttttt tagaattatt agaataaaag aaaataataa atagtataaa
    42961 tactagtacc cacatcatag ctcctttctt cttttctctt aacttgctta cattgtaaca
    43021 catctttttc tttttgacaa gtatagctgt caaaaagttt atgatttttg tcattttgca
    43081 aaagaaaaag gtcaggagta agttcctgac cactttatct atcattaata ctcttctaaa
    43141 atctcttcaa accacgtcag tttcatctac aacctcaaaa ccatgttttg agcaacctgc
    43201 ggctagcttc ctagtttgct ctttgatttt cattgagtat aaaatcctag tttttcaaag
    43261 atttctgaga agttttggct gattgtctca agtgacactt gcacttcttc tcgggtttgg
    43321 ttgttcttga ctgtcacttg tctgctttcg acttcgctct ctcctagggt gatgagggtc
    43381 ttagccgcaa agacatcggc tgacttgaac tgagctttga gcttacggtt gaggtaatca
    43441 cgctctgctt tgaaaccttg ttggcgaaga gcttggacta gttctagggc tttgacattt
    43501 gctccatcac ccaagactgc gatatagaca tctagggcat tttcgatagg gagggccacg
    43561 ccttgttttt caaggatgag aagcaggcgc tctacaccaa gcccaaaacc aaatccagca
    43621 gtttcagggc ctccaaagta agcaaccaaa ccatcgtagc gaccacccgc acagactgtc
    43681 aagtcattgc cctcaatctc tgtgataaac tcgaaaatgg tgtggttgta gtagtccaga
    43741 ccacgtacca tattggtatc gatgatgtaa tctactccaa gattttccaa catctgacgc
    43801 acagcatcaa aatgagtttg gctttcttca tcaagaaagt ccaagataga cggcgcattc
    43861 tctactgcca ccttgtcttc tttttcctta gagtccaaga cacgaagagg attttcctcc
    43921 aagcgacgtt ggctatcctt agacaaggtc tccttgagcg gtgtcaaata gtcaatcaag
    43981 gcttggcggt aggctgcacg gctctcagga tttccaagag tgttgaggtg caatttgaca
    44041 ccttgaatac cgatttcctt caaaaaatgg gctgccatag cgattgtttc cacatcggta
    44101 gctggattgc tagagccaaa acactcaaca ccaatctggt ggaattggcg caagcgccct
    44161 gcctgtggac gctcataacg gaacataggt cccatatagt agaacttgct tggcttttgc
    44221 acttctgggg cgaaaagttt attttccaca taggaacgga caacgggtgc agttccttct
    44281 ggacggaggg taatatgacg gtcacccttg tcataaaaat cgtacatttc cttggttacg
    44341 atatccgttg tatctccgac agagcgactg ataacctcgt aatgctcaaa aataggcgtg
    44401 cgcacttctg cgtagttgta gcgtttgaaa atttcacggg caaagccctc aacgtactgc
    44461 cacttggcag attcagcagg taaaatatcc tgcgttcctt ttggtttttg taatttcata
    44521 gggaatcctc tttaaactta atagtcttat tttaccataa atagagggat taaaacagtg
    44581 agaaaaaaat taggatttag atatcatttt tgagattaag aattgttaaa aaaatagcta
    44641 gcaaggaaag accaacaaat agcatccaag tcaactgcat gttccatacg gctactagtg
    44701 aaaaacaagc tgttcccaca ggtatggata aggtaaacaa tagacctaaa aaattactag
    44761 taggagctag aacctctgga gctagatttt tcatgagcat ggcactaatc tttggttgaa
    44821 ctttaccaga cacatacaga gtaaagaaga gaaatagcaa accaagcacg acttgattga
    44881 ataaattagc caaaccaaaa ggactgcttc ccaaaataat cattgcccgt aaggctactg
    44941 atgatgactg ctactaaaac acagaattga ttgataaata gtgcctctgt ataagaaaaa
    45001 ttcaagagag aatggctcaa aaagaagata ttataaattc cacccaaagc gccacccaag
    45061 gaattaataa gcaagacagc aaagagcata aaaccaaagt ttttctgtcc acttttaaga
    45121 aaaacgagac gtaaatttcg gtaaattgtt aggaactggt ctttgataga aagcttctca
    45181 ttttttagtt tttcaccatc agcagatgaa attgacaggc tcaatttgct ttttcctaaa
    45241 aaaagaataa aggctgatac gaggaaaaag caagcattaa ttcccgcaac gagggaaaaa
    45301 ttgttgaccg atagagctaa gagccagact ccgaaagctt gaccaccaat agctgaaata
    45361 taggtgatga actgtgaaaa agaataagcc tccatcagat catcttcagc tactttttcc
    45421 ttaataagag gcatacgcag gccacctgca aaatcactga tgatatcact aatgacattg
    45481 atcaaacaca ggctagaaaa ggcaaagaga ctagcttgct gaacaactag ggctgctaaa
    45541 aaaaatagaa ccgcctgaaa caaaccgcta tagaccatcc atttgacctt gtccctcgtg
    45601 taatctgccc gaatccctac aaaaactgta aagagggtcg gaagaatcat gacaatattc
    45661 gccatagcaa cagcaaaaga tgcttgtgac aaggtcgatg catagacgat aaagaccagg
    45721 ttgaaaatcg aagcaccaaa agcattgaag aagcgtgata aagtcaaaat tctataagct
    45781 ggatttctaa acaatagttt catagataat ctccctcctt gttctaagtt ctacatcaag
    45841 aatagcttta gtatagcact tatgaatttg cgaaacgatt cttttgaata tatgcaacaa
    45901 acaaaatgag caaaaaaacg atagaacttt tgtaatgacc tgagagaggg caacagttct
    45961 atcgcttcaa attttttctt ggtttgcaga tattcaagaa tcgagagttt ttctatagta
    46021 ttcggcagat ttattacagc ccagcatctc aaaaatacgg acagcatcct ccatcttttt
    46081 ctggccttcc ttgactctac cttgcttgct atcaaggaga ccttctgccc acagataaac
    46141 aattcggaaa taggtctcat tttccttgta gaaatgctct tcgataacac gtttaaaata
    46201 ataggcattg gtaaattctt cacactcaat actagctaaa aagccattca atagtatagt
    46261 atgaaaaagg tttcgattgc cagacatttc cattagaaaa tcagatttac gtaccatttc
    46321 tcgtacatat ctagtaaaaa gagaaacaga taaaaatgga gaactgactg aaaataaatt
    46381 gagttcatag attccccaga tctcggtaga aaacaaataa tcatgaagga cttttccttc
    46441 ctctgctgtt aactctaccc tttcatctat gctcttcata taagacttga taataatggc
    46501 atttagaata tgtttctgtt tgttgtgaga atgggcatgc ttttgatact ccctgcgata
    46561 taagtcctca agaggtgcta tattctttgg ttccaagaca tctgtaattt cttttctcaa
    46621 ctcagaatct gtatcatact ggaaacctct cgccagaaag aggatctcct ccacactggc
    46681 agatatattt tccagagcaa atagaaactt ttccaccgaa agctcactct gacctgtttc
    46741 aaaacgggac aacatagacg gcgaaaattg tcctccggtt gcttgtctca gtgagatatt
    46801 tcttgactct cgtaattgtc taaagacttt tccaatctgc tccatagact tccccttgat
    46861 tccgtatttt cttcatttta tcatattttt cagaaaattc atcaaaaact tgccaaattg
    46921 tcagaattat gagaaaatag aggatattta tcacgtggag ggactgctat gagagacgat
    46981 atcaaaatca atgaccgtgc tttggccttg caagaccaaa ttatcgaaaa actagagaaa
    47041 gtttttgata cagatgtgga attggatgtt tacaatctag gtctgattta tgaaatcaat
    47101 ctggatgaaa cggggctctg caagattgtc atgaccttca ccgatactgc ctgtgattgc
    47161 gccgaaagcc tgcctattga aattgtggca ggtctgaaac aaatcgaggg tatcaaagat
    47221 atcaaggttg aagttacctg gtcgcctgct tggaaaatca cacgaatcag tcgctatggc
    47281 cgtattgccc ttggactacc acctcgttaa gcaggccaat cacttttaaa gatgaaaaaa
    47341 gcaagccgaa aatggcttgc tttttgatac tcaatgaaaa tcaaagggca aactagaaaa
    47401 ctagccgcaa gttgctcaaa acactgtttt gaagttatgg atagaactga cgaagtcagc
    47461 tcaaaatact gttttgaggt tgtggataga actgacgaag tcagctcaaa acactgtttt
    47521 gaggttgtag atagaactga cgaagtcagc tcaaaacact gttttgaggt tgcagataga
    47581 actgacgaag tcagctcaaa acactgtttt gaggttgcag atagaactga cgaagtcagc
    47641 tcaaaacact gttttgaggt tgtagataga actgacgaag tcagctcaaa acactgtttt
    47701 gaggttgtag atagaactga cgaagtcagt aaccatacct acggcaaggc gacgttgacg
    47761 tgatttgaag agattttcga gtatgagttt attttttacc tgacttgtcc atattccaga
    47821 agtctgtcac ggctccgcgt gaagcagatg atacgatgtg ggcatattta ccgaggacac
    47881 cacggctgta aagtggtggc aaggttgttt ctgccttgcg tttttcaagt tcttcttcgg
    47941 atacggccat ggaaatttct ttggtatctt ggtcaaccgt aacgatatcg ccggtacgga
    48001 gataggcaat tggtccacca tcctgagctt caggagcgat atgtccaaca accagaccat
    48061 aagtaccacc agagaaacgt ccgtccgtca agagggccac cttatctccc tgacctttac
    48121 caacaatcat tgaagaaagt gacagcatct caggcatacc aggaccacct ttaggtccaa
    48181 caaaacgaac aacgactaca tcgccatcaa cgatttcatc tgtcaggacg gcctgaatcg
    48241 catcttcttc tgagtcaaag accttagctg gcccaacgtg acgacgcact ttaacacctg
    48301 ataccttggc aactgcaccg tcaggagcaa ggttcccgtt caagatgata agcggaccat
    48361 ccgcacgttt tggattttca agtggcatga taactttttg gcctggagtc aagtctgcaa
    48421 agtcagccaa gttttcagct acagtcttac cagtacatgt gatgcgatct ccgtgaagga
    48481 aaccatttgc caacaagtac ttcataaccg cagggacacc accgacttcg tagaggtctt
    48541 ggaagacata ctgaccagat ggtttcaagt cggccaagtg aggcacacgt tcttgaatcg
    48601 tattgaagtc ctcaagtgac aagtcaacat ttgcggcatg ggcaatggcg agcaagtgaa
    48661 gagtggcgtt tgtagaacca ccgagagcca tcgttacagt gatagcatct tcaaaggctt
    48721 cacgagtcaa gatatctgat ggtttgagac caagttccaa catcttaaca acagcacgtc
    48781 ctgctgcttc gatatcttct ttcttatcag ctgattcagc tgggtgagag gatgaccctg
    48841 gcaaactcat ccctagaact tcgatagcag ttgccatggt attagcagta tacataccac
    48901 cacaaccacc agggccaggg caggcattac attcaagacg tttcacgtcc tcagctgtca
    48961 tgtcaccgtg gttccatttt ccgatacctt caaagacaga aaccaagtcg atatctttac
    49021 catcaagatt tcccggtgca atagttccac cataggcgaa aatggctggg atatccatgt
    49081 tggcaatagc aatcatagat ccaggcatgt tcttgtcaca gccaccgata gcgacaaagg
    49141 catcgacatt gtgaccactc atagccgcct cgatggagtc cgcgatgatg tcacgagatg
    49201 ttagagagaa acgcatacca ggcgttccca tagcgatccc gtctgctacg gtaatagttc
    49261 caaactgtac aggccaagcg cctgcagatt tgacaccttc tttagccagt ttcccgaaat
    49321 catgcaagtg aatgttacat ggtgtatttt ccgcccaagt cgaaatcact cccacaatcg
    49381 gtgtttcaaa gtccttatct gtcataccag tcgcacgaag catagcacgg ttaggtgatt
    49441 taaccatgct gtcatagatg ctactgcggt gacgtttatc taattcagtc atcttatccc
    49501 tcccatttca gtttttacta ttatagcaca attttcgcat gaagaacaga ataaaattct
    49561 tgaattttca gaaaattcta tacacatgtg aaatatttaa aattaaaaac aacaaagcgg
    49621 attagtgcac tttctgatga ccagaatatg ctttttaatc cgctttcttt aaataacgta
    49681 atgtaatttt tacagaaatt ctttcagaac agtgtattta acatctatct tgcattataa
    49741 aactctagaa ccttctcttt tatattcgat tcactcaaac catactcatt aagaagataa
    49801 tccattttcc ctacttgacc gaatctttct tgaacaccca tccgatgaat ttttgttatt
    49861 ccatcatcag agaataattc acataaagca ctgccaattc cacctatctg attgtggttt
    49921 tctacagtaa atatagtttt tccacttaac attgttttta tctgttctgg tatcggtttg
    49981 attctaaata aatctatcac acctactgaa taacctaatt tagacagttc atctgcaact
    50041 cgaatacttg gagcaaccat tatgccagaa gcaacgatta caagatcttc accatgtctt
    50101 aactcaatgt agcctttaga aaaatcctct ccaccttgat acacaggaac tggagctttt
    50161 ctaattgttc gaatatattt tagtcctttt aagtctaatg tctggttcaa tatttcacga
    50221 aattggatat catcagttgc ttcaaaaatg atcgatttag gaattaaacg taacaatcca
    50281 atttcttcaa atggcatatg tgtcccacca ttcatttctg cagttactcc tgcatctgat
    50341 ccaatcacag tggcatccaa ttgtgcgtat ccaagagaaa taaataattg atcaaatact
    50401 cttcgtgaag aaaaaggacc aaatgtatga agataaggtc taaacccctg aatagacaag
    50461 cctgctgcaa ggccgaccat ttctgcttcc ataatcccaa cattcacata acggtctcca
    50521 aagtcctttt caagattatg agtagccatc gaacttgaca gatcggcttc taagactact
    50581 atatcagaat cactttgatt agcctctaaa aggaagtctc tatatacatg ccgtaattct
    50641 ttcgtacttc tcatcattct gtttcctcca attcctgact taatctttct acaactgaag
    50701 ttaacatttg tctctcctct acagtagggc gaagatgatg attggatttc atttcttcca
    50761 gctcttgaac cccttgacct ttaatagtat ctaatacaat gcacttaggt gatgaattat
    50821 ttgactgttt taattggaca atcccttcat aaatttctct aatatctgaa cccttgaccc
    50881 taatggattc aaatccaaat gctgaaaatt tttctacgaa atcacctgga ttacaaatat
    50941 cctttgtaaa accatctaat tgttttttgt tatcatcaac aaatacaatt aagttggata
    51001 actgttgatg agaagcaaac tgtatagcct cccaacattg tccctcattt aactcaccat
    51061 ctccaacaat agcataagta taaaagggac tctttcttat tctctgacca tatgcaagtc
    51121 cagttgcaac actaattcct tgtcctaaag agcccgttgt catatctatg cctggcgtta
    51181 gatttctatc aggatgagac ggtaatttgg ttccatttgt atttaaagaa tataagaatt
    51241 ctttgtcaaa gaaaccattc aaatagagtg tactgtatag agctggtcct ccgtgacctt
    51301 ttgataatat gaaataatct ctatctcgtg ctgcaaatat ttctggagtc attggcatta
    51361 tttcaccata aagcactgct aaaacttcta cgatagacag acttcctccg taatgtccga
    51421 atccaagatg attcaatgtt ctaagagtat ttaatcggat gttagtcgca aattttctta
    51481 acccatcttc tctattttta cttaaaatca tcccttattc ctccgttgca gatggctttt
    51541 taataaagga tactccaaac ataactgcta gaataagaac aagaccaatc acaatgcctg
    51601 cttgtgagcc aaattgattt aatattccta aaataattcc tgatagacca aaatctgcat
    51661 ctgagaaagt tgatccttgg aaaccaagtc ctcccaaaac tggcattaaa aagactggaa
    51721 gaaaactgat taaaatacct tgtaaaaatg ctccaatagt ggctccacga acaccaccag
    51781 atgcattccc aatgacacct gcagtcgctc cacagaagaa atgaggcaca acacctggta
    51841 agataacaac cgttcctgaa gcaatcataa ttgccatact tactaaacca ccaacaaaac
    51901 tagagataaa tccaattaga actgcattgg gtgcataagt ataaacaatc ggacaatcca
    51961 aagcaggttt tgaattaggt acaagacgct ctgaaatacc tttaaaggct ggaacaattt
    52021 cgcccaaaat aaggcgaaca cctgctaaaa taacaaatac ccctgctgca aattgacctg
    52081 ctaattgtaa agcataaact agaccacttg taccactact gatttctttt tctatatatt
    52141 ctgaccctgc aaagatagct acaataatgt aaataactgc catagataaa gtgatactaa
    52201 cagtactatc acgtaaaaaa gctaaactct ttggaaattt aatgtcctct gttgattttg
    52261 atttgtcacc gataaggcta ccagtaaaac cactcaacca atatcccaaa gaactgaaat
    52321 gacctaaagc caccttgtca tttccagtta attgaaccat atatttttgc acaaatgctg
    52381 gggaaatact cataataata ccgagtgcta atcctcctag taagatgaga ggcaagctag
    52441 taaagccagc aactgataaa atgaccgcaa tcatacatgc catatataga gtgtggtgcc
    52501 ctgttaaaaa gatatattta aatcgagtaa aacgagcgat taagatattg aacaccatgc
    52561 ctgcaaacat aatcattgca gtagctgagc catatgttgt taaagctaca gctacaattg
    52621 cttcattatt cggcacaacg ccagataaat gaaaagcatg ctcaaacatg gtaccaaatg
    52681 gatttaaaga attttgtaca attcctgcac caccagatac aactaagaaa ccaacaaagg
    52741 tcttaattcc acctttaata atatcaggta atttcttctt ctgaagaact aatcctaaga
    52801 ttgcaattaa agctactaaa atagctggtg tactaacaat atccaatatg aacttcatca
    52861 tgacgctagc ctcctatata agtccttttt cttcacaaat tttagtaatt aattctcgta
    52921 gttcgtccat atcaataata ctatttaaga tacgaacatc tccaagatga ctagctgaat
    52981 cagctagatc acgaccaaca atccaaatat cagctgcatt tggatctgct ccacctaaat
    53041 cataatgttc aacttctaca tccgaaacat tcaaatcact caatacagat tcaatattca
    53101 tctgtaccat aaaacttgaa cctaatcctg aaccacaagc tgtaccaatt tttaacatta
    53161 tctaatcctc ctgtttaatt atcattttaa tatcatcata gttttttgat gatattaaag
    53221 tttgaacatg atttttatct cttaaaattg ttgttaaatg tgacaaagcc tttaaatgac
    53281 tctcattatc aatggctgca atacaaatca acaatcttac ctcttgttct ggattatcca
    53341 ataaataaat cggttcttcc aaaactaaca ttgacattcc tatttcattc acaccttcat
    53401 ctggccgagc gtgaggaatt gctactccct tccctaaatt aataaaaggt ccaaactctt
    53461 ctactttttg aatcattgcc tcagggtagt tctccgttat cttatcttga tccaaaagcg
    53521 gtttagctgc taaacgaatc gcctccttcc atcctaattt ttgcgaacta acctgatagg
    53581 tttctttggt aataagttgt tctagcactg gtacaatttc ctttctatca tttttttgat
    53641 aaagataatt ctttaacgcc aatcttaatt ccaattcttg tgtaataatt ccatatcttt
    53701 tgacaatatt caggatttgt tcaatctcaa aatctccata ctctaaattc ggaaaatctt
    53761 ttaacactag ttctactagt tgtattgctt gctcttcagt catcataacc gaaactagat
    53821 aatttggctt ttctgtctcc acctttatgg tagaaaaaac catatcatag tcactactag
    53881 ctttcacctg taaatcatca atctttgagg ttcctataaa ctcaatttga ggaaataatg
    53941 ctaatagatt ctcttttaac atcaatgaag aactaacacc attaggacaa atgattgctg
    54001 ctttatacca tttttgaggc aaagtatctg ctttctttaa ataacctccg aaatggataa
    54061 caaaatatgc tgtttcacta tcaggtatgg gattgtcaat agcgtccatc aagggcatca
    54121 aagaatcttt gactaattca aataaatcag gataatgttc tttaacatgc aatacatatt
    54181 catttgaact aggtaggccg aactttaatc tatagtaagc cggtataagg tggcgacgaa
    54241 gattttctct caatccttcc ctttgtttaa aatgtaacaa agaaatatct tccattctac
    54301 ttataatagc ctctgttaat tgattaaagt aaaccggagc aacatctact tcaccttcaa
    54361 agcaacttga taataaaact gtgatatagc gataatcatc ctcagaaaac accgtatcta
    54421 taattcccaa atcaaccact gtatccaata aaatagtcgt tatatcttga ataacaggag
    54481 atactaatgt ctctgaaaga catactcttt caacatccct ttgataccta cacagaatga
    54541 atactaaacc gaaaaggtaa acttttaatt gattaacaat aggtactagc tgtagcttct
    54601 cataataatc tttaactacc tgatcaatca aatcataagt taatgaatac ccccaactgg
    54661 ataaaacata atccaaaccc caaatcccta tggaggattc cagcaactca ctaaccattt
    54721 gaaaagctaa acggtgctta ttccactctg aaccgtgtaa agtataacct tttgctctac
    54781 tgtaccctag ctccaaatca ttatctaaca taatctttct taatgattga atatcagata
    54841 aggttgtatt cttacttact ttcaaaaagt cttggtaatg attattcgat ataaaatcta
    54901 atcggcaaaa agtgtaaaga tagattaaag ctaagcgagt cgactttggt aaaaccaatt
    54961 catccgactt aataatatct gtcaaagact gcttcgtacg atttgataaa ctatagcgac
    55021 cttgtttttt atccagcact atccctttat tagctagata aggcactaaa taatctattc
    55081 cttctttgac ttcctttata ggtaagctca ccttaacaga taattcatat aatgatagct
    55141 cacaatgatc catcaaagtc atcaaaataa ctagtgctct ataatcaaac atttctcttc
    55201 c
//
LOCUS       DAVKQL010000003        54694 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVKQL010000003 DAVKQL010000000
VERSION     DAVKQL010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 54694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54694
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.003"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            181..519
                     /locus_tag="VW561_000114"
     CDS             181..519
                     /locus_tag="VW561_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEU9942460.1"
                     /translation="MTLTIHVYYKGDGDSAIKFAREMLDSGLVEEIRNQKGNLKYEYF
                     LPIEKEGTILLIDQWINQEALDEHHQSKTMQKILDLRKKYHLQMQVERYIEDDSGMPE
                     SDKSFIDTGN"
     gene            709..2180
                     /locus_tag="VW561_000115"
                     /pseudo
     CDS             709..2180
                     /locus_tag="VW561_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206493.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
     gene            2184..2423
                     /locus_tag="VW561_000116"
     CDS             2184..2423
                     /locus_tag="VW561_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family sugar transporter"
                     /protein_id="HEU9942461.1"
                     /translation="MIGSIAAILTTFAFLPQVFRIVKTKDTGSIALGMYVMQVIDIAL
                     WLAHGIRIGDLPLILANSVSFLLSGIILFYKLKYK"
     gene            2553..2867
                     /locus_tag="VW561_000117"
     CDS             2553..2867
                     /locus_tag="VW561_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEU9942462.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIAAAAFDLYLVSPQTKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
     gene            2985..4964
                     /locus_tag="VW561_000118"
     CDS             2985..4964
                     /locus_tag="VW561_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEU9942463.1"
                     /translation="MNVLNIKEKDILQFLIKNKERFVTSKELAEYLSCSDRTVRNVLK
                     LIEKTMIIQGVRLISKQGQGYQIFFENQGAYQEFRQTYELEEDYTKTAVSKGDDRLVF
                     ILNKLLFEQVPVLFDDLADELYVSRSTLSHDFRKIRVMLSEYNLSIESRANKGVYVSG
                     EERDKRRFIINYFLENQFFKTIHCYVKFNFFDQTVPLEEFARIVLDECQEANLKLSDF
                     VLQNLVVHIALSVIRLKSGFEIKNIDCQMTDDATERKVAQRILSKVREVTNQEFPVQE
                     IDYITLHLLAKSQQCQKNQKNISEEVLKKSLFKTFQNLGLDDMYNFSSDFQLIEGLIT
                     HLMTLQVRLESRITLNNPLVDEIKQNYSDIFFMTREILANMDKFLEWTISDDEVAYVS
                     LHFLAAMERSKESTKFNILAICATGFGAAQMLRNRLETEFGKRVEVVDVIGYYELNQE
                     KLKGIDFIVSAVDLSNLYFQIPVFKVSVFLKSDEMEMIRKAMDQMQVSSYVQSSKINK
                     FENNSFRQYFSKENFLICTESDKVNLLEKMVESLSVGESNEFEQSLLYGIKQREELSS
                     VVFSEKIAVPHPIQPFGTEGKVSVAICKDSLLWDNQSSHVQLVFLLSPSIYGNEGLAT
                     VTKKIVSLTENDELQNQLISCNNFEDFINIFEKIK"
     gene            4974..5288
                     /locus_tag="VW561_000119"
     CDS             4974..5288
                     /locus_tag="VW561_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEU9942464.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            5319..5825
                     /locus_tag="VW561_000120"
     CDS             5319..5825
                     /locus_tag="VW561_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942465.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIALFFFGNIYWL
                     LNQFISPSPIIIFPIMLIVFSILATIEQFKLYGNRKEKLGITLMFVRIQILISIGLLV
                     LTWTSWFKNLFPIFENNQVARLFVFVVLLLGLVLSLLDIRRIKKIYKRTDKAYQQFVQ
                     LEKNSLSL"
     gene            5905..7251
                     /gene="celB"
                     /locus_tag="VW561_000121"
     CDS             5905..7251
                     /gene="celB"
                     /locus_tag="VW561_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEU9942466.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNPLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NGVARFGTTGIFTAIIMAIVTVLIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVV
                     AFVVIFINGLLVAMGTDIFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
     gene            7631..7807
                     /locus_tag="VW561_000122"
     CDS             7631..7807
                     /locus_tag="VW561_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942467.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            8208..10421
                     /locus_tag="VW561_000123"
     CDS             8208..10421
                     /locus_tag="VW561_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEU9942468.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSQTGSFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(10643..11119)
                     /locus_tag="VW561_000124"
     CDS             complement(10643..11119)
                     /locus_tag="VW561_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEU9942469.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLQDQKSGPLGKRIEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            11342..14545
                     /locus_tag="VW561_000125"
     CDS             11342..14545
                     /locus_tag="VW561_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HEU9942470.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSSADASTRVIEAKDG
                     AITISSPEKLRAAVHRMVPIEAKKKYKLRFKIKTDHKVGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQLSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDAYTDRLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WMHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTVMYNAERGFGF
                     GLSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWSTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVDRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            14898..15274
                     /gene="tnpA"
                     /locus_tag="VW561_000126"
                     /pseudo
     CDS             14898..15274
                     /gene="tnpA"
                     /locus_tag="VW561_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(15662..16291)
                     /locus_tag="VW561_000127"
     CDS             complement(15662..16291)
                     /locus_tag="VW561_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEU9942471.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYVTLR"
     gene            complement(16301..17302)
                     /locus_tag="VW561_000128"
     CDS             complement(16301..17302)
                     /locus_tag="VW561_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEU9942472.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDIDSLFQGISHFHFSGITVAIGQEVRTILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEELFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(17333..17974)
                     /locus_tag="VW561_000129"
     CDS             complement(17333..17974)
                     /locus_tag="VW561_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEU9942473.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKGYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQIAHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(17993..18808)
                     /locus_tag="VW561_000130"
     CDS             complement(17993..18808)
                     /locus_tag="VW561_000130"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEU9942474.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            19080..19514
                     /locus_tag="VW561_000131"
     CDS             19080..19514
                     /locus_tag="VW561_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9942475.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFVR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            19526..20716
                     /locus_tag="VW561_000132"
     CDS             19526..20716
                     /locus_tag="VW561_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="unsaturated chondroitin disaccharide hydrolase"
                     /protein_id="HEU9942476.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            20727..21218
                     /locus_tag="VW561_000133"
     CDS             20727..21218
                     /locus_tag="VW561_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU9942477.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            21233..22012
                     /locus_tag="VW561_000134"
     CDS             21233..22012
                     /locus_tag="VW561_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9942478.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            21999..22817
                     /locus_tag="VW561_000135"
     CDS             21999..22817
                     /locus_tag="VW561_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9942479.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPTKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            22817..23110
                     /locus_tag="VW561_000136"
     CDS             22817..23110
                     /locus_tag="VW561_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU9942480.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            23132..25033
                     /locus_tag="VW561_000137"
     CDS             23132..25033
                     /locus_tag="VW561_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEU9942481.1"
                     /translation="MKEERRQFFERVDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYLVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSYYRAKYTLSNWGILQTIPMLAIYHFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEMLSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     RRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGMHY
                     IEGAYWSAEPDLPYLHRRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDIGVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            25093..26094
                     /locus_tag="VW561_000138"
     CDS             25093..26094
                     /locus_tag="VW561_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9942482.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(26204..26539)
                     /locus_tag="VW561_000139"
     CDS             complement(26204..26539)
                     /locus_tag="VW561_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942483.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(26550..26792)
                     /locus_tag="VW561_000140"
     CDS             complement(26550..26792)
                     /locus_tag="VW561_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942484.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(26808..27002)
                     /locus_tag="VW561_000141"
     CDS             complement(26808..27002)
                     /locus_tag="VW561_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9942485.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWKEDF"
     gene            27168..28118
                     /gene="rsmH"
                     /locus_tag="VW561_000142"
     CDS             27168..28118
                     /gene="rsmH"
                     /locus_tag="VW561_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEU9942486.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            28130..28447
                     /gene="ftsL"
                     /locus_tag="VW561_000143"
     CDS             28130..28447
                     /gene="ftsL"
                     /locus_tag="VW561_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEU9942487.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            28451..30703
                     /gene="pbpX"
                     /locus_tag="VW561_000144"
     CDS             28451..30703
                     /gene="pbpX"
                     /locus_tag="VW561_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HEU9942488.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGVT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKETAETFAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            30705..31685
                     /locus_tag="VW561_000145"
     CDS             30705..31685
                     /locus_tag="VW561_000145"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU9942489.1"
                     /translation="MFISISAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGIIFYLFYERGGDILSVFGYPVHLGFFYIFFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            31899..32039
                     /locus_tag="VW561_000146"
     CDS             31899..32039
                     /locus_tag="VW561_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942490.1"
                     /translation="MVDRTDEVSSKHGFEVVDKAKLMWFEEIFEECKKYWSVKVKILD
                     LF"
     gene            32295..34400
                     /locus_tag="VW561_000147"
     CDS             32295..34400
                     /locus_tag="VW561_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9942491.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEDLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGDSDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSQLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     TKHLEADMEDGVLVIREKA"
     gene            34852..35195
                     /locus_tag="VW561_000148"
                     /pseudo
     CDS             34852..35195
                     /locus_tag="VW561_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(35262..35744)
                     /locus_tag="VW561_000149"
     CDS             complement(35262..35744)
                     /locus_tag="VW561_000149"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEU9942492.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(35839..37323)
                     /locus_tag="VW561_000150"
     CDS             complement(35839..37323)
                     /locus_tag="VW561_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEU9942493.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFLNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            37476..39083
                     /locus_tag="VW561_000151"
     CDS             37476..39083
                     /locus_tag="VW561_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEU9942494.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGIGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIATIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDENKNAGFSTGQPWLAVNLNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLIRADFELLDTVDKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            39242..39415
                     /locus_tag="VW561_000152"
                     /pseudo
     CDS             39242..39415
                     /locus_tag="VW561_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
     gene            complement(39408..40253)
                     /locus_tag="VW561_000153"
                     /pseudo
     CDS             complement(39408..40253)
                     /locus_tag="VW561_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            40487..41932
                     /gene="cps4A"
                     /locus_tag="VW561_000154"
     CDS             40487..41932
                     /gene="cps4A"
                     /locus_tag="VW561_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4A"
                     /protein_id="HEU9942495.1"
                     /translation="MSRRFKKSRSQKVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLLFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSDIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKLIDLLGGIDVYNDQ
                     EFTAHTNGKYYPAGNVHLDSEQALGFVRERYSLADGDRDRGRNQQKVIVAILQKLTST
                     EALKNYSTIINSLQDSIQTNMPLETMINLVNAQLESGGNYKVNSQDLKGTGRTDLPSY
                     AMPDSNLYVMEIDDSSLAVVKAAIQDVMEGR"
     gene            41934..42665
                     /gene="cps4B"
                     /locus_tag="VW561_000155"
     CDS             41934..42665
                     /gene="cps4B"
                     /locus_tag="VW561_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4B"
                     /protein_id="HEU9942496.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLTESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVASDLVIAYGAEIYYTPDVLDKLEKKRIPTLNDS
                     RYALIEFSMNTPYRDIHSALSKILMLGITPVIAHIERYDALENNEKRVRELIDRGCYT
                     QVNSSHVLKPKLFGERYKFMKKRAQYFLEQDLVHIIASDMHNLDGRPPHMAEAYDLVT
                     QKYGEAKAQELFIDNPRKIVMDQLI"
     gene            42674..43366
                     /locus_tag="VW561_000156"
     CDS             42674..43366
                     /locus_tag="VW561_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein CpsC"
                     /protein_id="HEU9942497.1"
                     /translation="MKEQNTIEIDVFQLLKTLWKRKLMILIVALVTGAGAFAYSTFIV
                     KPEYTSTTRIYVVNRNQGDKPGLTNQDLQAGSYLVKDYREIILSQDALEKVATNLKLD
                     MPAKTLASKVQVTVPADTRIVSISVKDKQPEEASRIANSLREVAAEKIVAVTRVSDVT
                     TLEEARPATTPSSPNVRRNSLFGFLGGAVVTVIAVLLIELLDTRVKRPEDVEDVLKIP
                     LLGLVPDFDKIK"
     gene            43376..44065
                     /locus_tag="VW561_000157"
     CDS             43376..44065
                     /locus_tag="VW561_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEU9942498.1"
                     /translation="MPTLEISQAKLDSVKKAEEYYNALCTNLQLSGDGLKVFSITSVK
                     IGEGKSTTSANIAWAFARAGYKTLLIDGDIRNSVMLGVFKARNKITGLTEFLSGTTDL
                     SQGLCDTNIENLFVIQAGSVSPNPTALLQSKNFTTMLETLRKYFDYIIVDTAPVGVVI
                     DAAIITRNCDASILVTEAGEINRRDIQKAKEQLEHTGKPFLGVVLNKFDTSVDKYGSY
                     GNYGDYGKNKK"
     gene            44080..45447
                     /locus_tag="VW561_000158"
     CDS             44080..45447
                     /locus_tag="VW561_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU9942499.1"
                     /translation="MNGKIVKSSLAIIQSFLVILLTYLLSAVREAEIVSTTAIALYIL
                     HYFVFYISDYGQDFFKRGYLIELVQTLKYILFFALAISISNFFLEDRFSISRRGMIYF
                     LTLHALLVYVLNLFIKWYWKRAYPNFKGSKKILLLTATSRVEKVLDRLIESNEVVGEL
                     VAVSVLDKPDFQHDCLKVVAEGEIVNFATHEVVDEVFINLPSEKYNIGELVSQFETMG
                     IDVTVNLNAFDRSLARNKQIREMAGLNVVTFSTTFYKTSHVIAKRIIDIVGALVGLIL
                     CGLVSIVLVPLIRKDGGSAIFAQTRIGKNGRQFTFYKFRSMCVDAEAKKRELMEQNTM
                     QGGMFKVDDDPRITKIGRFIRKTSLDELPQFYNVLKGDMSLVGTRPPTVDEYEHYTPE
                     QKRRLSFKPGITGLWQVSGRSEIKNFDEVVKLDVAYIDGWTIWKDIEILLKTVKVVFM
                     RDGAK"
     gene            45478..46650
                     /locus_tag="VW561_000159"
     CDS             45478..46650
                     /locus_tag="VW561_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU9942500.1"
                     /translation="MKKSVYIIGSKGIPAKYGGFETFVEKLTAFQQDKAIQYYVACMR
                     ENSAKSGTTEDVFEHNGAICYNVDVPNIGPARAIAYDIAAINRAIEIAKENKDEDPIF
                     YILACRIGPFIHGIKKKIQAIGGTLLVNPDGHEWLRAKWSAPVRRYWKISEGLMVKHA
                     ALLVCDSKNIEKYIQEDYIQYQPKTTYIAYGTDTTRSTLKSSDEKVRSWFKEKNVSEN
                     EYYLVVGRFVPENNYESMIRGFLASNSKKDFVLITNVEQNKFYNQLLAKTGFDKDPRV
                     KFVGTVYEQELLKYIRENAFAYFHGHEVGGTNPSLLEALASTKLNLLLDVGFNREVGE
                     QSAIYWKKDELSQVIEKVEQFDAKMIDELDRQSNQRIADYFTWEKIVTDYEKVFKG"
     gene            46656..47552
                     /locus_tag="VW561_000160"
     CDS             46656..47552
                     /locus_tag="VW561_000160"
                     /inference="COORDINATES: protein motif:HMM:NF016852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HEU9942501.1"
                     /translation="MAVKLKYDKSDITFLDDDGLKKYQSELLKMISDVFTFFDENGIA
                     YSLSGGSILGAIRHKGFIPWDDDVDINIPRESYDKLFSLFESDNSLSRKYYLQSAKSH
                     PELGLHVSQIRKKGTVARRKYDHSAEECGISIDLYIVENVYNNPVKRFFQGYTSMFLT
                     FALASVRETKNHALMKEMFRLEGRKLNYSTGKLMVGWFFGIIPIEKWLNWLDKCNSSC
                     KDSHTKYVSIPTGRKHFRRETYLRENMNIYKKVPFETLMVNVPVWSEEYLEMFYGKDY
                     MVVPPVDKREQHLFLELSYGEE"
     gene            47553..48272
                     /locus_tag="VW561_000161"
     CDS             47553..48272
                     /locus_tag="VW561_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEU9942502.1"
                     /translation="MKVAILTASGIGSRIGQDIPKQFIHVENKPVIIYTLEKFQNHPE
                     IDEICIVILKGWEQMVKAYAEQFGITKLKMITLGGKSGQESIYNGLREVKKAHPNEDV
                     TVLIHDGNRPLVSNDIISNALATYQQFGNAVAAIPTTEVVFVLENPQSTSSTEALNRD
                     LLRRTQTPHVYHLDNILSLHEKALENGITDVAASCQLMQLFGEKSYFSLGTEKNLKIT
                     TVEDLDIFKALLSSTRDKWIK"
     gene            48275..49330
                     /locus_tag="VW561_000162"
     CDS             48275..49330
                     /locus_tag="VW561_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU9942503.1"
                     /translation="MTLLLNKIYRQDVESSLETIVNINDLNGKSILITGASGLIGSSV
                     IDQLLLLNEIRNFNIKIFCCGRKFESLEKRFGRQTEYLRFVFYDATKAPHFDFAVDYI
                     IHAASPASPELYVNQPVETMASNFLGMYNLLTYAKNAKVSNLVYISSSEVYGIGTSNN
                     PLEESYIGTVDHLSVRSSYASSKRATETLCASFASEYGIKIMIVRPGHIYGPSAKKSD
                     NRVSSFFMIEAIAGRDIIMKSTGSQLRSYCYSLDCASAILSVLLSGESGQAYNVSNPN
                     SIITIKQMAEIISRVGNVKLTMDIPSDQDIKQTNPMQNAGLSSDKLEELGWQGHFTAE
                     EGFQHTFNILKELSSEK"
     gene            49320..50303
                     /locus_tag="VW561_000163"
     CDS             49320..50303
                     /locus_tag="VW561_000163"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9942504.1"
                     /translation="MKSDSILIMMATYNGEEFLREQLDSILCQTISTWELLIRDDNSN
                     DQTRAIIKEYEEKDRRIKLIENISEYHGAYYNFWGLLNDVRQNENPFDFYMFADQDDI
                     WDVDKLERLISYYYRKVKTEEPVLIYADMRIIDANGKVIANSMDQLMGIRYTNPISTF
                     MAHKVYGCNTLFNHELFEILPLLPCYAPELAFLSHDNFTTKIAALKGHVYFYDEPTMS
                     YRRYGHNVTSKHEYNFTLKRILKRISKIDELAKDHALTYKQTLVATNLLHQQTSIDTE
                     LLFLDKVEKIIKKGGLNAVKMVWKEKIDWGNRVKTISRSLILASKLYKKYL"
     gene            50399..51544
                     /locus_tag="VW561_000164"
     CDS             50399..51544
                     /locus_tag="VW561_000164"
                     /inference="COORDINATES: protein motif:HMM:NF025665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HEU9942505.1"
                     /translation="MSKKEIKPICFYLPQFHPIPENDEAHGEGFTEWTNVKKAYPLYD
                     GHNQPRVPYNNNYYSLLDKEVLIQQAELAKKYGIYGFCYYHYWFKNGKKLLEKPVEMM
                     LENKDVDIPFCLSWANENWTRRWDGGNHEIIVEQNYDDLNDLEKHVDYLCQFFKDDRY
                     IKIDGKPLLLIYKPELIPNLVKTMEIIRNRARKNGFKDLEIIVQFPKFLLNNHQLELF
                     DGFAEFQPIFEIHMEKHERKNPVYRSVKSFLLKVGARNLVQKLEIRGEKKVHNKALLD
                     RRDYDATWTNIINRPIKDNKMIAGAFVDWDNTARNKNGRVFDGANPEKFEGYMRQLIE
                     KIQKEYQSEIVFINAWNEWAEGAYLEPDKKHGYGYLEALKTVIDETR"
     gene            51640..52167
                     /locus_tag="VW561_000165"
     CDS             51640..52167
                     /locus_tag="VW561_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016386641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HEU9942506.1"
                     /translation="MFPIELVSVGEKTYGGLYVLSFNKESKLKIGNFCSIAPEVAFIL
                     SADHYINQFSTYPFKSKVFDQGDEGVSKGDIIIEDDVWIGFRSTILSGVTIGQGAIVG
                     AGSVVTKSVPPYAIVGGVPAKVISYRFETEIREEMKKIDFSEFKLEKFKKVTEELYQP
                     ISSIRQINNINEKLK"
     gene            52234..53376
                     /locus_tag="VW561_000166"
     CDS             52234..53376
                     /locus_tag="VW561_000166"
                     /inference="COORDINATES: protein motif:HMM:NF016800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU9942507.1"
                     /translation="MFIAMLIYCQPEYLKSIPVTGRIYRDFMHVGIMFVTFSYISKKR
                     FPKFFVWLVLLYNSWLTFLTIQNNGDIKRAIFQLLLFTGFVSMIDIFSENMQDLIESL
                     LFTFEMVVYLNFVTVLFFPDGFFHRVNDAYGITKEWFLGADNNFLFTLFPATLIALLY
                     KNLGGVKIRAYSLISIILATQLIKGSGTAKVGIVALILFDLVPLVKYILTPYRGLVIS
                     STLSFLIVVTGKVEFLRGIVEGILEKDLTFTSRTFIWNNAMSAIREKPFGYGMMMFTD
                     NVHILGNFPGWIWKGATHAHNNFLQVLFQGGYLGFGVYFLIILKGAMLCRKYWDDRNA
                     RLLFYGLFIFTIISITENLEMQMMYLVIILPFLLPNILVKERGKNE"
     gene            53369..>54694
                     /locus_tag="VW561_000167"
     CDS             53369..>54694
                     /locus_tag="VW561_000167"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012745.1,HMM:NF017515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9942508.1"
                     /translation="MNRGILVSIIVPIYNQEEYLNISLPTLKQQTYKNLEFILVDDGS
                     TDSSKQIINEYLNADSRFKYVYKENGGLVSATIAGVKEASGDYLAFLDPDDKLGLDYI
                     ENFIKELDCDYDFIAAGFFYDDGKLIKPYYLKENSVLTQSDIKKLRTDFLSDTGGLAI
                     SNRVFISRWNKLYKTNLVKEIVKEFRKFEMVSLGEDTIFTFLLLQKAQLGKTIALSNS
                     YFYNVGNPNSMMKEGAIINYVEKCRIAYQSLTKLLIERNLTNEMSCQLYYFLVLSFFN
                     RLEKEDMESFKKIYDILKRDSLFLKILFSLQKKANKKQKLQLLMIRLCKSGKQYLNIK
                     RVVSKLHAKLKLFCQNFKLVRKQIFRKNLVQLAYFLKFQQQRKIGFDDMKVFLPIIEE
                     RLKEDLLEFQKLEVLPSTIEKNIFVFWWDGFDEIPEIVSICLASLKKNYP"
BASE COUNT        17371 a         8934 c        11712 g        16677 t
ORIGIN      
        1 tttgtcctgc tcttgtttca attgactata aatcgctata agaaaaaatc attttattgg
       61 tataaagaag tgattgaaag taatggcgaa acgttagata attagttcat aaaaaacaag
      121 tttagatcct catctaaact tgttttttta aaattgaagg atgattttga aggatataag
      181 atgacattga ctattcatgt atattataaa ggtgatggag atagtgcaat aaagtttgca
      241 agagaaatgc ttgatagtgg ccttgttgaa gaaattagaa atcaaaaagg taacttgaaa
      301 tacgaatatt ttttaccaat tgaaaaagag ggaactattc tccttattga tcagtggata
      361 aatcaagaag ctttagatga acatcatcaa tcaaagacaa tgcagaaaat cttagattta
      421 agaaaaaaat atcacttgca gatgcaagtt gaacgttata tagaagatga ttctgggatg
      481 ccagaaagcg ataagtcgtt tatagacaca gggaattaga attattgtga gagtttttgt
      541 agtttgaaag tattgaaaat cgtaaagata tcattacgag aatacaagaa gatattgttc
      601 tatttccatt aaaaattact tctttcctta acaataagga aaaagaatgc gttttcatct
      661 tttggtttga ggtttataat aagagtatca aagtcaggag gaaaaaagat gactactttt
      721 aaagatggat ttttatgggg tggtgctgtt gctgctcatc aacttgaagg tggatggcaa
      781 gaaggtggca agggaattag tgttgctgat gttatgactg ctggtcgtca tggagtagct
      841 cgtgaaatta cttcgggagt tttagagggt aaatattatc caaatcatga ggcgatagat
      901 ttttatcatc gttataaaga agacatagca ctttttgctg aaatgggatt caagtgcttc
      961 cgtacctcta ttgcatggac gcgtatcttt ccaaaaggtg atgagttaga gccgaatgaa
     1021 gaaggattac agttttatga taatcttttt gatgaatgct taaagaatgg tattgaacct
     1081 gtcatcactc tatctcattt tgaaatgcct tatcacttag tgaccgaata tggtggttgg
     1141 aaaaatagga aattgattga tttctttgct cgttttgcag aagtcgtatt taaacgttac
     1201 aaagataagg ttaaatattg gatgactttc aatgaaatca ataatcaagc gaattatcag
     1261 gaagattttg caccatttac taactcaggt attgtatatg aggaaggtga taatagagaa
     1321 gcaattatgt atcaagcagc acattacgaa ttagttgctt ctgcacgagc tgtaaaaatt
     1381 ggtcatgaga ttaatccaga ttttcaaata ggttgtatcc aatttatcca gctacttgca
     1441 atcctaagga tatcttaatg gcaatgaaag ctatgcagaa gcgttattat tttgctgatg
     1501 tgcatgtttt aggtaaatat cctgagcata ttttcaagta ttgggaacga aaaggtattt
     1561 cagttgattt tgctgcccag gataaagaag atttacttgg tgggactgta gattacattg
     1621 gtttcagtta ctatatgtcc tttgctatcg actctcatcg tgaaaataat ccttattttg
     1681 attatcttga aacagaagat ttagtgaaaa ataattatgt taaggcttct gaatgggagt
     1741 ggcaaattga tccagaaggt ttacgttatg cgttaaattg gtttacagac cactatcact
     1801 taccactctt tattgttgaa aatggttttg gagctataga tcaagttgca gcagatggta
     1861 tggtacatga tgattataga attgaatatc taggtgccca tattcgtgaa atgaaaaagg
     1921 ctgtagttga agatggtgtt gatttaatgg gttatactcc atggggatgt attgatttgg
     1981 tttcagctgg taccggtgaa atgcggaaac gttatggttt tatttatgta gataaagatg
     2041 acaatgggaa gggaagttat aatcgttccc cgaaaaaatc ttttggctgg tataaggaag
     2101 ttatttcatc taacggtgaa tcagtagaat atgctaaaca aactgagatt attctcagtt
     2161 tgtttagcat attgggatga catatgattg gaagtattgc agcaatttta acaacatttg
     2221 catttttacc acaagttttt cgtatagtta agacaaagga cacaggatct attgctctag
     2281 gtatgtatgt tatgcaagtt attgatattg cattgtggct tgctcatggt attcgtatag
     2341 gagacttgcc tttgatttta gcaaatagtg tttcatttct actctcagga ataattttat
     2401 tttataaatt gaagtataaa taattttttt ccatatcatt taggaaaatg agggaattgt
     2461 cattggaaac gctttatttt ataataaaaa tagataacag ataagtcaac ttagcacagc
     2521 tttgattgga tttaagaaaa ggagaaaata ttatggcaaa agctttaatt atttgtgcag
     2581 caggaatgtc ttcatcactt atggcacaga aaacaacaga gtttctaaaa ggaaaaggga
     2641 aagatattgt agttgatgct attagtgcta ctgaaggagg gaaagctatc gcagcagcag
     2701 cttttgattt gtatcttgtc agccctcaaa caaaaatgta ctttaagcaa tttgaagagg
     2761 ctggttctaa agcaggaaaa ccagttgtac aaatacctcc tcaagcctat attcctattc
     2821 caatgggaat tgaaaaaatg gctaatttga ttcttgagaa tatttgatga acacatgaaa
     2881 tgtcaataaa tatttaagtt aagataaatt gttttgagtt tgatataaat tgatttaagt
     2941 gtataatatg cttcaatata tgaaaacata cgttttagga gggaatgaat gtgttaaaca
     3001 ttaaagagaa agatatcctt caatttttaa taaaaaataa ggaacgtttt gttacaagca
     3061 aagaactggc agaatattta tcctgttcag atagaactgt ccgtaatgta ctgaaattaa
     3121 ttgagaagac aatgattatt caaggagtcc ggttgatttc aaaacaaggt caagggtatc
     3181 aaatattttt tgagaatcaa ggcgcttacc aggagtttag acaaacttat gaactggaag
     3241 aagactatac gaaaactgcg gtttcaaaag gtgatgatcg tttagtattt atcttaaata
     3301 aattattatt tgagcaagtt ccagttcttt ttgatgattt agctgatgaa ctttacgtta
     3361 gccgttcaac attatctcat gatttcagaa agatacgagt gatgttatca gagtataatt
     3421 tgtccattga gagtagggcg aataaaggag tttatgtctc aggcgaggaa cgtgataaac
     3481 gacgttttat cataaattat ttcctagaga atcaattttt taaaacaata cattgctatg
     3541 taaaatttaa cttttttgat caaactgttc cattagaaga atttgctaga attgttttgg
     3601 atgaatgcca agaggccaac ctaaaactat ctgattttgt actacagaac ttagtagttc
     3661 acatagcatt gtctgtgatt cgtttaaaat ccggatttga aataaaaaat atagactgtc
     3721 agatgactga tgatgcaaca gagagaaagg ttgcccaaag aattctttct aaagtaagag
     3781 aagtaacaaa tcaggaattt ccagttcagg aaatagacta cattactttg catttgttag
     3841 ctaagagtca acaatgtcaa aaaaatcaga agaatatttc tgaagaagtt ttaaaaaaat
     3901 ctttatttaa aacatttcag aatttaggat tagatgatat gtataacttt tcttcagatt
     3961 tccaactaat tgaaggattg ataactcatc taatgacact acaagtacgt ttagaaagcc
     4021 ggattacctt aaataaccct ttagtagatg aaattaaaca gaattatagt gatatattct
     4081 ttatgactag agaaattctt gcaaatatgg acaagttctt ggagtggaca atatcagatg
     4141 atgaagtagc atatgtaagt cttcactttt tagctgctat ggaacgtagc aaagagagta
     4201 ctaagtttaa tattcttgct atttgtgcaa caggatttgg tgcagcccaa atgttaagaa
     4261 atcgtttgga aacggagttt gggaagcgag tagaagttgt tgatgtaata ggctattatg
     4321 aattgaatca agaaaaactt aaaggaatag attttatagt gtcagctgtt gatttatcaa
     4381 atctatattt tcaaatccca gtttttaaag ttagtgtgtt tcttaagagt gatgagatgg
     4441 aaatgattag aaaagctatg gatcagatgc aagtttctag ttatgtacaa tcatcaaaga
     4501 ttaataaatt tgaaaacaac agctttagac agtattttag taaagaaaat tttttaatat
     4561 gtactgaatc agacaaggta aatttacttg aaaagatggt tgagagttta tctgttggtg
     4621 aatcaaatga gtttgaacaa tctttacttt atggaattaa acagcgcgaa gaattaagtt
     4681 cagttgtttt ttctgaaaaa attgcagttc cgcatcctat tcagcccttt ggtacagagg
     4741 gaaaggtttc ggttgcaatt tgtaaagatt cgttactctg ggataatcaa agttcacatg
     4801 tccagctagt atttcttcta tctccatcaa tatatgggaa cgaaggtttg gcaactgtga
     4861 ctaaaaagat agtctctctg acagagaatg atgaattgca aaatcagcta atatcttgca
     4921 ataactttga agattttatt aacatatttg agaagataaa ataggaggtt catatgaatc
     4981 aagaggaact gcaggttgca gcatttgaaa ttatcctgca ttcgggaaat gcaaggtcag
     5041 aaatacatga agcatttgca aaaatgagag aaggtagttt tgatgatgca gaaagtaaat
     5101 taaatcaatc aaatgagata attcttgaag cgcatcatgc acaaaccaaa ctcttgcaag
     5161 aatacgcaag tggagtggaa attaaaattg aaattatcat ggtacatgct caagatcatt
     5221 taatgaccac tatgacatta ttagaagtag caaaggaaat gttagctctt tataagaaag
     5281 ttaactaaat atgtgttgtt atataaggag gaaatgatat ggataaaaaa gaacgacaaa
     5341 agattgaaca acaacgtcga gagatggctc taactaatac tttttttaat cgatatcttc
     5401 ttctacgtta ttcaatagca ctcttctttt ttgggaatat ttattggctt ttgaatcagt
     5461 ttattagtcc ctcgcctatt attattttcc ccattatgct catagtattt tctattttag
     5521 caactattga acaatttaaa ctttatggaa ataggaaaga aaagttggga ataacactaa
     5581 tgtttgttag aatacaaata cttatttcaa taggattact tgttttaact tggacaagtt
     5641 ggtttaagaa tctttttccg atttttgaaa ataaccaagt agcacgttta tttgtttttg
     5701 tagttcttct attaggttta gttctgagtt tgttagacat tagaagaatt aaaaaaattt
     5761 ataaacgaac agataaggct tatcaacaat ttgtccaatt ggaaaagaac tcacttagtt
     5821 tataatcctg atctagtata atttgtattt gaaactagtc tttagaaagt aaaaattatt
     5881 atgttttatt aaggagttct atttatggat caacaaaacg ggttgtttgg ttttcttgaa
     5941 aaccatgtta tgggaccaat gggcaaactt gctcagttta aagtagtgcg tgctatcacg
     6001 gctgcaggta tggctgctgt accatttact attgtaggat caatgttttt ggtattcagt
     6061 attttgccac aagctttctc attttggcca attgtggcag atattttctc tgcttcattt
     6121 gataaattca catcacttta catggttgca aactatgcga ctatgggttc tctatctctt
     6181 tatttcgttc tatcacttgc atatgaattg acaaaaattt atgcagagga agaagaactc
     6241 aatatgaatc ctcttaatgg tgccttgctt gccttgatgg cttttgtcat gacagtaccg
     6301 caaatcattt ttgatggtgg aatgatgaag actgtgacaa gtctaaaaga aggtgcagta
     6361 attgcagatg gatgggcaat gggaaatgga gtcgcacgtt ttgggacaac agggattttt
     6421 accgcaatca ttatggcaat tgtgactgtt cttatttatc gtatgtgtgt taaacataat
     6481 tgggttatta aaatgcctga agctgttcca gaaggagttt ctcgtggatt taccgctttg
     6541 gttccgggat ttgttgttgc atttgttgtt atctttatca acggtcttct tgtagcaatg
     6601 ggaacagata tttttaaagt cattgcaatt ccatttggtt ttgtatccaa tctgactaat
     6661 tcgtggattg gtttaatgat tatttatcta ttgactcaac tactttggat tgtaggtatc
     6721 cacggtgcga acattgtttt tgcatttgtt agtccaattg ctcttgctaa catggctgaa
     6781 aatgctgctg gcgggcactt cgctgttgca ggtgaatttt ctaatatgtt tgtaattgca
     6841 ggtggttctg gtgcaacttt aggactatgt ttatatattg cttttgcctc taaatctgaa
     6901 cagcttaaag caataggacg agcatctgta gttccggcct tatttaatat taatgaacca
     6961 ttaatttttg gattacctat tatctataat ccagccttgg ctataccatt tattttagca
     7021 ccaatggtta ctgctactat ttattacgta gcgaattctc taaactttat taagccaatt
     7081 atcgcacagg ttccatggcc aactccagta gggattggag ctttcttagg gacagcagat
     7141 cttcgagctg tattagttgc tctagtatgt gcatttgcag cattcctagt ctatcttcca
     7201 ttcatccgtg tatatgatca aaaattggtg aaagaagagc aaggtatcta ataagtaaaa
     7261 aagtatactc gatgatagaa tccatatagt ttaaataatg agtgttaata ttagcagtga
     7321 agaggtcttt ttaagatatt cttctcttct ttttcatatt ttttcgctat gtagggtatg
     7381 aagcaaaata gaaagtagtt cgaatagttg ataaacagga aactgctgtt atttaaaaaa
     7441 aacaataaag taaagtatgt tatcgtagaa tacgatacaa tagtaaaaaa gcaaaaagaa
     7501 tgcgttttat atttgggttt gaggtttata aaataggata agcgtgctga aaacttagat
     7561 atgacaaaca aaataaaatt tttaaaagct acatagaaac atggtataat gtatctaaag
     7621 gaggtagcat atgtccttag atatagataa agaaaagatg acaatcatgg gaatagcttt
     7681 tgaaaacaga tccgttttta agagtgtttg gtatgcttta agtacaaata tgattgaagg
     7741 atggcgacct acggttagtg atgttgaaaa attacgagat gaagctcttg ctctggggat
     7801 gacttaatga agcgcaactg ttatgagtct tatgactatg ttgatccgga taatttatat
     7861 accgatccgg gttcttcagt tctaagaaat aagcaggaag gaaaaaagca cgcgagcttg
     7921 aatatcgtat gattgctagt aagagtttga aattatttat caatcctatc ttagttaata
     7981 gtttaaaagt agtttgtttc acaactgcat aagcttagaa aagttgaaaa gtgataactc
     8041 tttaataaac tcttggtttg ttaaaagata ttttgtccac tacatttttt caattgacta
     8101 ttatttgtca tagatataac agatagaaca ttgcattcag aataatgaaa aacgcatcaa
     8161 aaatggtgcg ttctgttttt atgctaccat aaaaggagga aaacaaattg aaaattttta
     8221 agggagagtt ttatcgaatc tctgtattaa cagacaagct agtaaggtta gaatactctc
     8281 aaactggaag ttttgaggat agaacgacac aacttatcta taatagagat tttggccaag
     8341 tttcgttaga ttatatcgag acatcaaacg tactagatat tatgacggac tattttcatc
     8401 tgcactttaa taaaggagaa tttaacgccg aaaatttatt tatagaatta aaaggaaatt
     8461 ttgccgtata tggtagtcgc tggtattttg gtgaatctat tgaaacgtta aaaggaacag
     8521 ctcggactct ggataaggca gatggagcaa tctcgttaga agatggaatt attagccgaa
     8581 atggtatagc cttattggat gattctcaag gatttatttg ggatgaacaa tctggttata
     8641 ttgagagaga aaatcaaatt gacctgtatt tctttgccta tgggcatgat tatagaggag
     8701 caatcagaga cttttaccat ttgactggtt caacaccctt gttgccaaga tatgctttag
     8761 gcaattggtg gagtagatat tggccttata cgtcggatga atatttggat ttaatagaca
     8821 gatttgaaac agagaaaatt ccattatcta tcggtgtgtt agatatggat tggcatataa
     8881 ctgacattcc agctcgtttt ggaagtggct ggacaggata tagttggaat agaaacttaa
     8941 taccaaatcc agaacagtta ttgcaacaac ttcatgatag aaagttaaaa ctctccttaa
     9001 atgtccatcc tgctgatggg atacgggctt atgaagaagc ttatcctcaa atcgcaaaac
     9061 ggttggggtt aaatgtagaa ctagaagaac ctgctatttt tgattttttt aatccctctt
     9121 ttagggaagc ctactttaaa gatgttcatt atgagctaga aaagcaggga gtagattttt
     9181 ggtggattga ctggcaacaa gggacacaag gtatgctaga tccactttgg cttttaaacc
     9241 attatcacta tcaggatagt tgtaaaaatg cagaaggtgg tttgatttta tcaagatatg
     9301 caggtcctgg tagtcaccgc taccctgttg gtttttcagg ggatactatt attagttgga
     9361 attccttaag atttcaaccc tattttacag cgacagcatc taatatcggt tatagttggt
     9421 ggagtcatga tatcggtgga catatgttgg gggattatga cgaagagcta caaactagat
     9481 ggctacagtt tggtgttttt agtccgatta ctcgattaca tagttctaga agtcctttta
     9541 atagtaaaga accttggttt ttttcagaaa caacatctaa gattatgaag aaataccttc
     9601 gtttgagaca tcagatgatt ccttatctat ataccatgaa tgtaaagaca catgaggaag
     9661 gtgccccatt aatcagtcca atctattatt tctatccaga gaatgatgag agctataatg
     9721 ttccaaacca atactttttt ggaacagaac tgatggtggc tcccattgta gaaaagatgg
     9781 atttgacatt ccaatctgca aaagtagatg tatggttccc tgaaggtgaa tggtatgact
     9841 tcttttcaga gaaaaaatac acaggtggtg tgaagttaag tgtttatagg gacatctcga
     9901 ctacgcctgt gtttgcaaaa agtggtgcaa tcattccctt ggttggttct gagataggta
     9961 tgggtgttga tttacctgag gttgtagatt ggtatgtatt cccagggaaa caacattctt
    10021 ttgaaatgct tgaagatcaa aatggtcaaa gatataaaac aagattatca atcgattggg
    10081 aaatgggaat ggtagagtta gcattacaag gagattctag tatcgttcca agcaatagaa
    10141 aacatagaat tcattttaaa ggaacgaatg tgtctataat tgaattgcca aataagaatg
    10201 atacagctaa atttgaatgg aaagataata aaaggacatc tttaaatgac gaagttttta
    10261 gactactaaa gacagcttct cttccatatg aattaaaaga tagattgtta aatcaattca
    10321 tcaatgccaa aaactctcat gacttaatga atatcttgca tcatcaggat aaggaattga
    10381 gagggcgttt gttggaaatg atatttacta gccaaaacta attgaaacaa ttagaaagtc
    10441 atttgtttag tgtatatgaa gattctgtat ggtattcgtt atcactggtc acattgaaaa
    10501 tgtgaaataa gcacctacaa catagacttt acaacacttc atgtttcata tcatggggtg
    10561 tttttctatt taagtcaaag ccaatcaaaa aatctcacta ttcatcaggt taaaggaaac
    10621 ctaatcatag tgagattgtg aattatagta gagtttgtat cgcctcttca atttgttttg
    10681 ggtctgtttt tgaagaaaag cgttcaaaga cttgcccatc tcgaccgatg agaaacttag
    10741 cgaaattcca ttcgattcgt tttcctagtg ggccagattt ctggtcttgt aaccagacat
    10801 agagagggtc tgcttcctta ccgttgacct taatcttggc aaaacgtggg aaggtggttt
    10861 gaaaatgtag gctacagaag gcgttgattt cctctgcgct gccgggtgct tgtcccataa
    10921 actgattgca agggaaatcc aatatttcaa agccctgatc ttgatagcgt tcatagagtt
    10981 cttgaagtcc ctggtactgg ggcgttaaac cacatccagt agcagtgttg acaatcaaga
    11041 gaactttgcc acgatagcta tccaagggag ttgcttggtt gttttggttc aaaacggaaa
    11101 aatcataaag tgaagtcatg agggcctctt ttctttttct acattgtaac agtttttact
    11161 tttttcgtcg agtcggatgg aagatattgt tgaagaataa gccctcaata tgatagaaac
    11221 cgtttacaaa taactttaaa aatggtataa tttaattaaa ccggtttact tagttgctat
    11281 aaatctctaa tttgagattt agttcctatt gattttacaa tatgtttatt ggagtgtata
    11341 catgcaaaca aaaacaaaga agctcattgt gagtttgtct tcacttgttt tatcaggatt
    11401 tttattaaac cattatatga cagttggagc ggaagaaacg actacgaata ccattcagca
    11461 aagccagaag gaagttcagt atcagcaaag ggatacaaaa aatttagttg aaaatggtga
    11521 ttttggtcag acggaggacg gaagcagtcc gtggacagga agcaaagctc aggggtggtc
    11581 agcttgggta gaccagaaga atagttctgc agatgcctca actcgagtca ttgaggctaa
    11641 ggatggggct atcactatct caagccctga gaaattaagg gcagcggttc accgtatggt
    11701 tcctattgaa gctaagaaaa agtataaact gcgtttcaag attaaaacag atcataaagt
    11761 cgggattgcc aaagttcgta tcattgagga aagtggtaag gacaagcgat tgtggaattc
    11821 tgcaacgacg tcaggaacaa aggactggca gaccattgaa gcagactata gcccgacttt
    11881 agatgttgat aaaatcaagc tggagttatt ctatgaaaca ggaactggga ctgtttcctt
    11941 taaggatatt gagctggtag aggtagcaga ccagctttct gaggattctc aaacagataa
    12001 acagcttgag gaaaagattg atttaccaat tggaaaaaaa catgtttttt ctcttgcgga
    12061 ctatacttat aaggtagaaa atcctgacgt tgcttcagtc aaaaatggaa ttttagaacc
    12121 tcttaaggaa gggacaacca atgtcattgt cagtaaagat ggcaaggaag tgaaaaagat
    12181 tcctttgaag attctagcct ctgttaagga tgcatacaca gaccgtttgg atgactggaa
    12241 tggcatcatt gctgggaatc aatactatga ttctaaaaat gaacagatgg ccaaattaaa
    12301 ccaggaattg gaaggaaagg tagctgatag cctatctagt atttcaagtc aggcggaccg
    12361 cacctatttg tgggaaaaat tttcaaatta taaaatgtct gcaaatctga ctgccactta
    12421 tcggaaattg gaggagatgg ccaagcaagt gaccaatcct tcttctcgtt attatcaaga
    12481 tgaaactgtc gttcgaacag tcagggattc catggaatgg atgcataaac atgtctacaa
    12541 tagtgaaaag agcattgttg ggaactggtg ggattatgaa atcggtacac ctcgtgccat
    12601 caacaatacc ttgtctctga tgaaagaata cttctctgat gaggaaatta aaaaatatac
    12661 agatgtgatt gaaaaatttg taccagatcc cgaacatttc cgaaagacga ctgataaccc
    12721 attcaaggct ctaggtggaa acttagttga tatgggaagg gtaaaagtaa tagctggttt
    12781 actgcgtaag gatgatcaag aaatttcttc taccattcgc tcgattgagc aagtgttcaa
    12841 gttggtagac caaggtgaag gtttttatca agatggatcc tatatcgacc ataccaatgt
    12901 tgcctatacg ggtgcttatg ggaatgtttt gattgatggc ctgtctcaac tgttgccagt
    12961 cattcaaaag accaagaatc caatcgataa agataaaatg caaaccatgt accactggat
    13021 tgataaatcg tttgctcctt tgctggtgaa tggagagctg atggatatga gtcgtggacg
    13081 ctcgatcagt cgtgcaaata gcgaggggca cgtggccgca gtagaagtac taagagggat
    13141 tcaccgaata gcggatatgt ctgaaggaga aaccaaacaa cgtttgcaga gtcttgtgaa
    13201 gaccattgtt caatcggata gttattatga tgtctttaag aatttgaaga cttataagga
    13261 tatcagtttg atgcaatcct tgttaagtga tgcaggagtc gcaagtgttc caagaacaag
    13321 ttacctatct gcctttaaca agatggataa aacagtcatg tacaatgcag agagagggtt
    13381 tggatttggc ttgtcactct tttccagtcg taccttgaat tacgaacaca tgaacaagga
    13441 aaataaacgt ggttggtata cgagtgatgg gatgttctat ctttacaatg gcgatttgag
    13501 tcactatagc gatggctact ggtcaacagt taatccatat aagatgcctg gtacaacaga
    13561 gacggatgct aagagagcgg atagcgatac aggtaaagtt ctaccgtctg ctttcgttgg
    13621 aacgagcaaa ctagatgatg ccaatgcgac agcaaccatg gatttcacca actggaatca
    13681 aacattgact gctcataaga gctggtttat gctgaaggat aagatcgcct ttttaggaag
    13741 caatatccaa aacacttcaa cagatactgc tgcaactaca attgaccaga gaaaactgga
    13801 atcaagtaat ccatataaag tctatgtcaa tgataaagaa gcctccctta cagaacaaga
    13861 aaaggattat cctgaaaccc aaagtgtctt tttagaatcg tccgattcga aaaagaatat
    13921 tggttacttt ttctttaaga agagttcaat cagtatgagt aaggctttgc aaaagggagc
    13981 ctggaaggat atcaatgaag gacagtcaga caaggaagtt gaaaatgaat ttcttacgat
    14041 tagtcaggct cataagcaaa atggagattc ttatggctat atgctaattc ctaacgtgga
    14101 tcgtgccacc ttcaatcaaa tgataaaaga gttagaaagt agcctcatcg aaaataacga
    14161 aacccttcag tctgtttatg atgctaaaca aggagtttgg ggcattgtga aatatgatga
    14221 ttctgtctct actatttcca accaattcca agttttgaaa cgtggagtct ataccattcg
    14281 aaaagaaggg gatgaatata agattgccta ctataatcct gaaacccagg aatcagctcc
    14341 agatcaggaa gtctttaaaa agctagagca agcagctcag ccacaagtac agaattcaaa
    14401 agaaaaggaa aaatctgaag aggaaaagaa ccattcggat caaaagaatc tccctcagac
    14461 aggagaaggt cagtcaatct tggcaagtct agggttcttg ctacttgggg cattttatct
    14521 attccgtaga ggaaagaaca actaatttgt tcatcatcta gatgaattac atgaaactgt
    14581 ggaaaataga aaaaatccga agaaggccaa atatcttcgg atttttcttt taaaaaagga
    14641 taagttgata cttatccttt tttacttcat tattatgtga ctatagcgtt ttgaaactag
    14701 aatagaacgt tataactgct aaaagatttc tataaattca tttgattttc ctaatcaatt
    14761 tgttcgtatc ctatttcact ccactatata agtccgtctc gctccgtgag gtgcgcgtcg
    14821 aaggttatac caaaaaaact ccaaacgcga tacaataaag gtattcaatc caattgtaaa
    14881 gcgaaaggag aaaaaatatg gcacaaaaga ctcatagttt atcatacaca aagtggatgt
    14941 gttctatcat attgtgttca cccctaagta tagacgaaaa gtcatctata atcaatatag
    15001 aagtagttta ggcgaaatat ttcatcgctt gtgtagttat aaaggtgttg aaatgatcga
    15061 gggtcactta atgccagacc atgtacacat gttagtcagt attccactaa ggctaagtgt
    15121 ttcaagtttt atggggtatt taaaaggcaa aagtgcactc atgatgtttg acaaacacgc
    15181 caatctcaag tacaagtttg ggaatcggca tttctgggcg gaaggttatt atgtaagtac
    15241 agtagggctt aataaagcca caattaagaa atatagtcaa aattaaagaa attatcgagt
    15301 ggatgatttc ttcacgagta tgaaaatttg agaactagta aagtatgata tatagtaaaa
    15361 tgaaataaga acaggacaaa atcgatcagg acagtcaaat cgatttttaa caatgtttta
    15421 gaagtagagg tgtactattc cagtttcaat ctactatagc actagataaa ttgagtgtaa
    15481 aagaatatga ggatcccttt agggatagtg gtaagtaata ccaaagtctc ttaaagaggc
    15541 aagtgacgag tcaagagcaa taaggcttga acaacgtgaa agccagcgtc tttaggcgct
    15601 ggctggtaat ttgggcttat agctctggga taaaccaccc gtttgacggg tggttatgat
    15661 tttatctgag tgtaacatac tgttgggcaa tctcgctgat gcggtcaaag ttgccttggg
    15721 aagcgagttt attgagttcg ccaccaattc caacggcatc tgcaccagca gcgaaccatt
    15781 gagggatgtt gtttagaccg actcctccgg ttaccattac ggaaacttgt ggaatcggtg
    15841 ccttgactgc agagatatat gctggactga gagtactacc tgggaagagt ttgatgattt
    15901 cactaccggc ttcaagtgca gtcgtgatct ctgtgagggt aatacagcct ggaatgtacg
    15961 gtgtgctgta gagattgcac attttcgcag tttcagcatg gaaagatgga gaaacaacgt
    16021 aatttgctcc agctagaatg gcatctctag cagttacggc atcaagcaca gtacctgcac
    16081 cgatacaaac actctgatcg tcctgataca agtctacaag ttccttgatg atttgtcctg
    16141 catactgatt ggtataggcg atttcaatag ctttgatacc gcccttgata caagcaatcg
    16201 aggcttgcag tccttcttcc tttgtatttc ctcgaataac agcgacaatt ttttgttttt
    16261 ttagttcaat aatcgtatct gatttggtca tgtaattctc ctaacgaatg atatcttgtg
    16321 catttgccag taaattttca atactagttg cggaagtgga gagatggtct cctggaagag
    16381 tgcatttgag agttgcgctc gcaactgcaa agtcaatggt agtttttagg gaggaatgat
    16441 ggagtagttg gtaaagggca ccagatataa aggcatcccc gctaccaatt cgttgataga
    16501 ctgcagtttt tagttggaca gactcttcaa atagttcttc tagagcatag gcttgataga
    16561 catttttgtc ttgctcatca ctagagcgga gggtatggaa aatggccttg aaaccatagg
    16621 cttcttttaa aagtcgcatg cgattttcca cctcttctag gctagcactg tctcttggaa
    16681 acatctctag attttggtca tcaatcatga gaggatcaat accgaagcaa tagtcagtaa
    16741 aacgtgcaaa cttagaaaat tcatacttgg cttctaggac tgaaatcatc tttgttctca
    16801 gattgagatc cattgaaacg acaattcctc ggcgcttggc ttcttccaag agtaggagaa
    16861 ggatcgtacg gacctcttga ccgatagcta cggtgattcc actaaaatga aaatggctaa
    16921 tcccctgaaa gagagaatct atatctagca tgtttggccg aatctggctg atactcgtat
    16981 gcttacgatc gtagaaaact tcactttgac gacaaccaaa gccgttctcc aaatagtaga
    17041 ggccgattcg atcgccaagc cgacagattg aactggtatc gatttggtgc tgtttcaaga
    17101 atgtgagaaa acgatctcca atctcgttgg cagggagtgc ggtaaagact ttcgttgaga
    17161 tacccagggc ttgcaaatta caagcgatgt taatttctga tccgccaaaa taagtcgaac
    17221 tggcaacatg atcgccgata ctggtggcat ctaatggtga gattcgaatt aatggttcgc
    17281 caaacagtaa tattttcgtc atccattagt tcctttctgg tttagaatag ctttacttgg
    17341 ctaatacttc tttcaaataa gcagcgactt catcatcttg gcagttttca aagaagtatt
    17401 cttggaagta tttgccagag atggtgtctt tgaggaagtc ttggtcgatt gttttaagga
    17461 tggtcatcaa atcattgtgg gcgatttgtt tcacctcgtt taagatacga gcgttgcgtt
    17521 gttcagggat tacacgttct cttgggtagc caccgcccat ttcttcagca aataagcgtt
    17581 caaacatcaa tttgagggtc agttctgccc cccagccaaa tcctttggca taagggatag
    17641 acaaggcgtt accaccattg atttgagaat aaaggtaagc gtcagttggg tccactgcta
    17701 gaccacagac aacaccaggg aagctgttta aagcaagcat agcccctaca cccgtaccac
    17761 agccggtaac aacaaagtca actgcctttg tatttaaaag gatggcagcc attagtccgt
    17821 tctgcacata agttaattga ctttctcctt cttctccacg cataccatag ttaaataatt
    17881 ggtagccttt tttatctgtc gcttctttta gactatcgta aataatgtga ttcttgctag
    17941 cttgactatt ttcattgatt aatgcgattt tcataagatc ttctttctat ttttactcag
    18001 gttgttttcc gatgtaggct aagataccgc catctacata taggatgtgg ccattgacaa
    18061 aattgctggc atcactagcg agaaagacag cagggcccat caaatcttca ggatttcccc
    18121 aacgtgcagc aggtgttttt gcaatgatga actggtcaaa tgggtggcga gaaccatctt
    18181 cttgcaactc acgaagaggt gctgtttgag gagtggcaat ataacccggt ccaattccgt
    18241 tacattggat attggctcca ccgtattcag acgcaatgtt gcgggtcaac attttcaagc
    18301 cccctttagc agcagcataa gcgctaactg tttcacgtcc cagttcgctc atcatcgaac
    18361 aaatattgat aatctttcca tgccctttct ttatcataga aggaataact gcctttgaaa
    18421 cgataaatgg tgcgtttaag tcaatatcga tgaccttacg gaaatcagcg gcgctcattt
    18481 cgcacattgg aactcggcgg ataataccag cgttattaac gaggatgtca atgacaccaa
    18541 cctcttgttc gatttgcttg accatggctt ggataccgtc ctcgtctgtc acatcacaga
    18601 catatccatg ggcttcgatg ccaacttcac gataagcttc aatcccttta ttgaccagat
    18661 cttgattgat atcgttaaag acaatagtag cgccggcttc agcgtaggat ttggcaatag
    18721 caaatccaat tccataagaa gcgccggtga tgagagcaat ttttcctttt aaagaaaact
    18781 gctcaattga gaatgatgta tttgtcattt gatacttcct ttcaaatttg ttaagacttc
    18841 tctataacca aattatattc tagtaaaccg gttttgtcaa taaataaata tttttattta
    18901 taaaaaggtt tgttttccta aaaaaacgga agattttgaa atttctgcct attaaaatgg
    18961 ttaaaaagat aatgaaagcg ctaatatttt tttaaaaaaa ctattgacaa gataaaaata
    19021 aaggattaca atggggaata taaagtaaac cggtaaacct aaacaagaaa ggagaaaaga
    19081 tgaaaattgt acttgtaggg catggacatt ttgctacagg gatttatagt tctttacaat
    19141 tgattgcagg caatcaagaa aatgtggagg caattgactt tgtggaagga atgtcagcag
    19201 atgaactcaa gcaaaaaatc ttacttgcaa tttcaaatga agaagaagtt ttaatcctaa
    19261 gtgatctctt gggaggatcg ccattcaagg tttcttctac cataatggga gaaaatccag
    19321 ccaaaacaat gaatgttctc tcgggtttga acttagccat gttaatggaa gcagtctttg
    19381 ttagaatggc tcatagcttt gatgaagttg ttaataaatc agtagtggcg gcccagggcg
    19441 gagtcgtaaa tggtaaagaa ttgttttcaa cggatgcaga ggaagaggaa gaagatttcg
    19501 aatcgggtat ttaaagggta aaagaatgat aaaaaaggtt acgattgaaa aaataaaatc
    19561 gcctgagcgc ttcttagaag taccacttct gacgaaagaa gaagtcggcc aggcaatcga
    19621 taaggttatt cggcagttag aactcaacct tgactatttc aaggaagatt tcccgacacc
    19681 agctaccttt gataatgtct atccaatcat ggataacacg gaatggacca atggtttctg
    19741 gacaggagaa ctgtggttgg cttatgaata cagtcaacag gatgcattta aaaacatcgc
    19801 tcataaaaat gttctttctt tcctggatcg tgtcaataag agagtagaat tggatcacca
    19861 tgatctcggc ttcttgtaca caccgtcttg tatggctgaa tataagataa atggagatgg
    19921 agaggctaga gaagcaacct tgaaagctgc agataagttg attgaacgct atcaagaaaa
    19981 aggtggtttt attcaagctt ggggagactt gggcaagaaa gagcattacc gtttgattat
    20041 cgactgcttg ctcaatatcc aactcttatt ctttgcttat caagaaacag gcgatcaaaa
    20101 atactacgat attgcagaaa gccatttcta tgcttcagct aataatgtaa tccgtgatga
    20161 cgcttcgtcc ttccacacct tctattttga tcctgagaca ggtcaaccct ttaaaggtgt
    20221 aacgagacaa gggtatagtg atgattcatg ctgggcacgt ggtcagtcat ggggagtcta
    20281 tggtattcct ttgacttatc gtcacttaaa agacgagtcc tgctttgact tgtttaaggg
    20341 tgtgaccaat tatttcttga atcgtctgcc aaaagatcat gtgtcctatt gggatttgat
    20401 ttttaatgat ggtagtgatc aatcacgaga ttcttcagca acagctatcg ccgtctgtgg
    20461 gattcatgaa atgctaaaac atctcccaga ggtggatgct gacaaagata tttataaaca
    20521 tgctatgcat gccatgcttc gttccttgat cgaacattat gcaaatgatc aatttacccc
    20581 tggtgggaca agtctcctcc acggtgtgta ctcatggcat tcaggtaaag gagtggatga
    20641 aggcaatatc tggggtgact actattacct agaagccctt atccgtttct acaaagactg
    20701 gaacctatat tggtaggagg agaaatatga caatgccaaa tattattatg acccgtatcg
    20761 atgaacggtt gattcatggg caaggacaac tttgggtaaa atacctaggt tgtaatacgg
    20821 tcattgttgc caatgacgaa gtaagcacag acaagatgca acaaactctg atgaaaacag
    20881 ttgtgccaga ctcagttgcc atgcgtttct tccctttgca aaaggtgatt gatatcattc
    20941 acaaggctaa tcctgctcaa acgatcttta tcgttgtaaa ggatgtgaag gacgctttaa
    21001 ccttggtaga aggtggtgtc cctatcaaag aaatcaatat tgggaacatt cacaatgcta
    21061 ctggtaaaga gcaagtgaca cgctccatct tcctgggtga agaggacaag gcggccctca
    21121 aggaattgag ccaaactcat caagtaacat ttaatacgaa aacaactcca acaggaaatg
    21181 atggagctgt tcaagtcaac attatggact atatttaaaa gaggagatcg ttatgtcgat
    21241 taatgtattt caagcgattt taattggatt atggacagct ttctgtttta gtggaatgct
    21301 gttaggaatt tacaccaata gatgtattgt tctgtcattt ggtgtcggaa ttattctagg
    21361 tgatctgcct actgctcttg caatgggagc tattggtgaa ttggcttata tgggattcgg
    21421 tgttggtgct ggaggtactg ttccaccaaa cccaatcgga cctggtatct ttggtacctt
    21481 gatggctatc actagtgctg gtaaagtcag tccagaagcg gctcttgccc tctctactcc
    21541 gattgctgtg gcgattcaat tcttacaaac tttcgcctac actgtacgtg ctggtgcgcc
    21601 tgaaacggcg atgaagcact tgaaaaacca taatttgaag aaatttaagt ttactctaaa
    21661 tgcaacaatt tggttgtttg cctttattgg atttaccttg ggttgcttgg gtgccctttc
    21721 aatggatacc ttgttgaaac tcgtagacta cattccaccg gtattgctta caggtttgac
    21781 agttgctggt aaaatgctcc cagctatcgg ttttgcgatg atcttgtcag tgatggctaa
    21841 gaaagagttg attccctttg tcttgttggg atatgtttgt gcagcttatc taaacatccc
    21901 aacaattggt attgcaattg taggtactat ctttgctttg attgaatttt ataacaagcc
    21961 aaaaacagcg gatcatgtgg tagaggagga agcacacgat gactggatct aacaaattaa
    22021 caaaacgtga ttatcttaaa acgtctttgc gggcattctt ttgtcaaaat ggatttaact
    22081 atagtaacta tcaagggttg ggatatgcca atgtgatgta tcctgctttg aaaaaacact
    22141 atggagagga tcaggaaggt ttctaccaag ccttggaaga aaactgtgaa ttctataata
    22201 ccaacccaca cttcctgcct tttattacca gcttgcatct tgtaatgttg gaaaatggcc
    22261 gaccaacaaa agaaacacgt agcatcaaga tggccttgat gggaccattg gcaggtattg
    22321 gggattctct ttctcaattc tgtttagctc cattgttctc aaccatcgca gcttcgtttg
    22381 ctcaagaagg cttggttgtc ggtccaatct tgttcttcct tgcgatgaat acgattttaa
    22441 cagcgattaa attgtcaact ggtctgtatg gatacaaact aggaacaact gtgattgata
    22501 aactaagcga acagatggca acgatttctc gtattgccaa tattatcggt gtaaccgtaa
    22561 ttgctggttt ggcagcgaca tctgttaaga ttatggtgcc gattaccttt gctgcagggg
    22621 aagttaaagc agacgctaaa caaagtatcg taagtattca gggaatgctt gataaggttg
    22681 ctccagctct tctaccagcc ctatttacac ttttagttta ctacttgatc aaagaaaaga
    22741 aatggacaac atataaactc gttattttaa cagttatcat cggaattatc ggaagctggc
    22801 ttaagattat agcttaatgg atacaacatt gttttatgga atagtgattg tcttggcagt
    22861 gagtcccctt ttactgtcaa gctttcattc tattcgtcag caaaagttgc ttcgcaaaca
    22921 gatggagcaa cgacaagagt atttagcttc tttaacatct ggtgatgaag tgttgttatt
    22981 gtctggaatt catggaaaaa tcatttctat caaagatgac ttggtctcct tgcagattgc
    23041 aaaaggtgtg gtcatctatg tagaaaagga aagtgtaatg ggaaagacaa aagaactgct
    23101 ttttaagtag ttctgtttct ggtaatagat aatgaaagaa gaaagaagac aattttttga
    23161 aagagtcgat ggaaaccaat gtcgtgatta tatcttgtcc cactgttcaa aagactatga
    23221 gaaagtcaag agttcccttg aacgcttgat ggacaatcgt tttatgtttg atagtccttg
    23281 ggatatggag ccttgttcaa aaatccatca aatccagccg atggtatggg atcaagtatt
    23341 tgaagatgat ccagaatggt cttatatgct caatcgacaa gaatatctct tgcagtttat
    23401 gatagggtat ctggtagaag gagataagga ctatattcaa aagtgcaagt tctttctatt
    23461 tgattggatt gagcaggtga gagaattttc tcctcaatcc ttgatgacta gaaccttgga
    23521 tacgggtatt cgttccttta cttggttgaa actactcttg ctcctcttga aatttgactt
    23581 gctagaggag aaagaactag agaaaatttt ggtcagtcta gaaaagcaga ttgactttat
    23641 gaaaagctac tatcgcgcca agtacaccct tagtaactgg gggattttac aaacaattcc
    23701 gatgcttgct atctatcatt tcttttcaga taagatggat ctagaagaag cttaccattt
    23761 tgcttcagag gagttgaaac agcaaattga gacacagatt ttaggagatg gaagccagtt
    23821 tgaacagtcg attctctatc atgtagaagt ttataaagcc ttgctggatt tgtgtctctt
    23881 gcttccagac ttgcaagata gttaccaaga gttgctggaa aagatggcga cctatattca
    23941 aatgatgaca ggcttagatg gacggacttt ggcttttggt gatagcgatt ctacagaaac
    24001 gacagaaatg ttgagcctgt ctgctgtggt tttgaacaag gaagaccttc ttaacggtct
    24061 ggatgttaaa gttgatttgc ttagcctctt gttcctaggg cgagaaaagg tcaagcgact
    24121 gcaggaattt gaaaggagag cttggcagcc taagtccatg atctttgaag actctggaca
    24181 tgtctgcatt aaggatgaac atcgttatct atttttcaaa aatggtccgc taggaagtgc
    24241 ccatagccat agtgacgaga atagtttttg cttacagtat caaggccaac ctattttcat
    24301 agatgctggg cgttattctt atcgggagat atatgaacgt tatctcttaa agagtgcttg
    24361 gagtcattcg acctgcattg tagatgggaa agctccggaa agaatcacag gatcctggga
    24421 atatgaatac tatcctcact ccctgttttg tcaccataaa gaaagggagg gaatgcatta
    24481 tattgaaggg gcttattggt cagcagaacc tgatttgcct tatcttcaca gaagaaaaat
    24541 cctcatgttg gtagaggatg tctggctctt ggtggatgac atcaggtgtc aaggtcagca
    24601 tgaggcgttg actcagttta tccttgacaa ggatgtgacc tatcaagatg ggaaaatcaa
    24661 tcagttgaga ctatggagtg aagttgattt tgatttggaa gataccatca tttctcctaa
    24721 atacaatgag cttgaaagaa gtagcaaact caccaagcgc caattctttg agaatcagat
    24781 gctggattat accatcattg cgcatgagag ttttgaaatc atccgtcatt ctgtctacca
    24841 gacagatgat cgtgaagtgg aaaatgctct ggcttttgaa gtgaaaaatg acgaaacaga
    24901 caagctgatt ctgttattaa gcgaggatat tggtgtaggt gaaaaattgt gcctcgttga
    24961 cggaacaaaa atgcgtggaa aatgtctagt atatgataaa ataaatgaga gaatgattcg
    25021 cttgcagtgc tagaaatagg cattttgaat agtgaatatg ttataataag tattagtagg
    25081 aggtgtttta gattggagaa gaaactgacc ataaaagaca ttgcggaaat ggctcagacc
    25141 tcgaaaacaa ccgtgtcatt ttacctaaac gggaaatatg aaaaaatgtc ccaagagaca
    25201 cgtgaaaaga ttgaaaaagt tattcatgaa acaaattaca aaccgagcat tgttgcgcgt
    25261 agcttaaact ccaaacgaac aaaattaatc ggtgttttga tcggtgatat taccaacagt
    25321 ttctcaaacc aaattgttaa gggaattgag gatatcgcca gccagaatgg ctaccaggta
    25381 atgataggaa atagtaatta cagccaagag agtgaggacc ggtatattga aagcatgctt
    25441 ctcttgggag tagacggctt tattattcag ccgacctcta atttccgaaa atattctcgt
    25501 atcatcgatg agaaaaagaa gaaaatggtc ttttttgata gtcagctcta tgaacaccgg
    25561 actagctggg ttaaaaccaa taactatgat gccgtttatg acatgaccca gtcctgtatc
    25621 gaaaaaggtt atgaacattt tctcttgatt acagcggata cgagtcgttt gagtactcgg
    25681 attgagcggg caagtggttt tgtggatgct ttaacagatg ctaatatgcg tcacgccagt
    25741 ctaaccattg aagataagca tacgaatttg gaacaaatta aggaattttt acaaaaagaa
    25801 atcgatcccg atgaaaaaac tctggtattt atccctaact gttgggccct acctctagtc
    25861 tttaccgtta tcaaagagtt gaattataac ttgccacaag ttgggttgat tggttttgac
    25921 aatacggagt ggacttgctt ttcttctcca agtgtttcga cgctggttca gccctccttt
    25981 gaggaaggac aacaggctac aaagattttg attgatcaga ttgaaggccg caatcaagaa
    26041 gaaaggcaac aagtcttgga ttgtagtgtg aattggaaag agtcgacttt ctaaaatgaa
    26101 ggaaaatgac ttgcaatttc tgttaagaaa taaaataatc ccacctagaa caagctaggt
    26161 gggattattt gcctatgaaa tgagaaatta tgggagcaag ctcctaaatc aactgttttt
    26221 gatctacttc tttaactact tgataaaagt tatagaagta ggccaaactt gaaatgatgg
    26281 ttacgactag gaatattgaa aatttccatt ggacagggtt ggttaaaagt tgtggaaagg
    26341 atatgaggag aaagaagagg gctgcgttga ggacaggtat ccgttttgat tgtattttct
    26401 caagtccttt attgagcgca ggaagaaaga ggagtaggag tagtaaaact gtatgagaaa
    26461 cagctcctga aataagggcg aagaaaagga aaatactgat aaaaacatga atgatcagta
    26521 gtctagctag tgatttcata aggcacctcc taatcctggt cttttttagc tcttgcaata
    26581 cgaagtgagt cgacaatatg tatcatcact ccgaaaaaga aagctcccag tatagtttta
    26641 aaaatatgtt ttgtatttag aagagaactg ataaaatttg gattttcact tgttagggta
    26701 tcaatgagtg gaattataaa aaatatcact gttccataaa tcgaacctgc tttcagacca
    26761 ggataacgta actgtttctt ttcttttttc atgagtttgc tcctttttta aaaatcttcc
    26821 ttccaaaaga gactgttgag gtcagtttgg aggctgcggg cgagattgag acagagttcc
    26881 agagttggat tgtacttgtc gttttcaatc atattgatgg tctgtctcga gacaccgata
    26941 tccttggcga gctcgagctg ggaaatgccc agttccttgc gaaattcttt cacacgattc
    27001 atctggtctc ctttctgatt tgtgtcgtat atatttgact ttattatagt cttttaaaca
    27061 taaagtgtca agtatttttg acatattttt tgaagaaata gtagtctcct tgtcctattt
    27121 gtctgacaag tgcaagctgg tcggatttgt ggtaaaatag ataagatatg acaaaagaat
    27181 ttcatcatgt aacggtctta ctccacgaaa cgattgatat gcttgacgta aaacctgacg
    27241 gtatctacgt tgatgcgact ttgggtggag caggccatag cgagtattta ttaagtaaat
    27301 taagtgaaaa aggccatctc tatgcctttg accaggatca gaatgccatt gacaatgcgc
    27361 aaaaacgctt ggcaccttac attgagaagg gaatggtgac ctttatcaag gacaacttcc
    27421 gtcatttaca ggcacgtttg cgcgaagctg gtgttcagga aattgatgga atttgttatg
    27481 acttgggagt gtctagtcct caattagacc agcgtgagcg tggtttttct tataaaaagg
    27541 atgcgccact ggacatgcgg atgaatcagg atgctagtct gacagcctat gaagtggtta
    27601 atcattatga ctatcatgat ttggttcgta ttttcttcaa atacggtgag gataaattct
    27661 ctaaacagat tgcgcgtaag attgagcaag cgcgtgaagt gaagccgatt gagacaacga
    27721 ctgagttagc agagattatc aagttggtca aacctgccaa ggaactcaag aagaaggggc
    27781 atcctgctaa gcagattttc caggctattc gaattgaagt caatgatgaa ctgggagcgg
    27841 cagatgagtc catccagcag gctatggata tgttggctct ggatggtaga atttcagtga
    27901 ttacctttca ttccttagaa gaccgcttga ccaagcaatt gttcaaggaa gcttcaacag
    27961 ttgaagttcc aaaaggcttg cctttcatcc cagatgatct caagcccaag atggaattgg
    28021 tgtcccgtaa gccaatcttg ccaagtgcgg aagagttaga agccaataac cgctcgcact
    28081 cagccaagtt gcgcgtggcc agaaaaattc acaagtaaga gggaaaaaga tggcagaaaa
    28141 aatggaaaaa acaggtcaaa tactacagat gcaacttaaa cggttttcgc gtgtggaaaa
    28201 agctttttac ttttccattg ctgtaaccac tcttattgta gccattagta ttatttttat
    28261 gcagaccaag ctcttgcaag tgcagaatga tttgacaaaa atcaatgcgc agatagagga
    28321 aaagaaaacc gaattggacg atgccaagca agaggtcaat gaactattac gtgcagaacg
    28381 tttgaaagaa attgccaatt cacacgattt gcaattaaac aatgaaaata ttagaatagc
    28441 ggagtaagat atgaagtgga caaaaagagt aatccgttat gcgaccaaaa atcggaaatc
    28501 gccggctgaa aacagacgca gagttggaaa aagtctgagt ttattatctg tctttgtttt
    28561 tgccattttt ttagtcaatt ttgcggtcat tattgggaca ggcactcgct ttggaacaga
    28621 tttagcgaag gaagctaaga aggttcatca aaccacccgt acagttcctg ccaaacgtgg
    28681 gactatttat gaccgaaatg gagtcccgat tgctgaggat gcaacctcct ataatgtcta
    28741 tgcggttatt gatgagaact ataagtcagc aacgggtaag attctttacg tagaaaaaac
    28801 acaatttaac aaggttgcag aggtctttca taagtatctg gacatggaag aatcctatgt
    28861 aagagagcaa ctctcgcaac ctaatctcaa gcaagtttcc tttggagcaa agggaaatgg
    28921 gattacctat gccaatatga tgtctatcaa aaaagaattg gaagctgcag aggtcaaggg
    28981 gattgatttt acaaccagtc ccaatcgtag ttacccaaac ggacaatttg cttctagttt
    29041 tatcggtcta gctcagctcc atgaaaatga agatggaagc aagagcttgc tgggaacctc
    29101 tggaatggag agttccttga acagtattct tgcagggaca gacggcatta ttacctatga
    29161 aaaggatcgt ctgggtaata ttgtacccgg aacagaacaa gtttcccaac gaacgatgga
    29221 cggtaaggat gtttatacaa ccatttccag ccccctccag tcctttatgg aaacccagat
    29281 ggatgctttt caagagaagg taaaaggaaa gtacatgaca gcgactttgg tcagtgctaa
    29341 aacaggggaa attctggcaa caacgcaacg accgaccttt gatgcagata caaaagaagg
    29401 cattacagag gactttgttt ggcgtgatat cctttaccaa agtaactatg agccaggttc
    29461 cactatgaaa gtgatgatgt tggctgctgc tattgataat aatacctttc caggaggaga
    29521 agtctttaat agtagtgagt taaaaattgc agatgccacg attcgagatt gggacgttaa
    29581 tgaaggattg actggtggca gaatgatgac tttttctcaa ggttttgcac actcaagtaa
    29641 cgttgggatg actctccttg agcaaaagat gggagatgct acctggcttg attatcttaa
    29701 tcgttttaaa tttggagttc cgacccgttt cggtttgacg gatgagtatg ctggtcagct
    29761 tcctgcggat aatattgtca acattgcgca aagctcattt ggacaaggga tttcagtgac
    29821 ccagacacaa atgattcgtg cctttacagc tattgctaat gacggtgtca tgctggagcc
    29881 taaatttatt agtgccattt atgatccaaa tgatcaaact gctcggaaat ctcaaaaaga
    29941 aattgtggga aatcctgttt ctaaagatgc agctagtcta actcggacta acatggtttt
    30001 ggtagggacg gatccggttt atggaaccat gtataaccac agcacaggca agccaactgt
    30061 aactgttcct gggcaaaatg tagccctcaa gtctggtacg gctcagattg ctgacgagaa
    30121 aaatggtggt tatctagtcg gggtaaccga ctatattttc tcggctgtat cgatgagtcc
    30181 ggctgaaaat cctgatttta tcttgtatgt gacggtccaa caacctgaac attattcagg
    30241 tattcagttg ggagaatttg ccaatcctat cttggagcgg gcttcagcta tgaaagactc
    30301 tctcaatctt caaacaacag ctaaagcttt ggagcaagta agtcaacaaa gtccttatcc
    30361 tatgcctagt gtcaaggata tttcacctgg tgatttagca gaagaattgc gtcgcaatct
    30421 tgtacaaccc atcgttgtgg gaacaggaac gaagattaaa aacagttctg ctgaagaagg
    30481 gaagaatctt gccccgaacc agcaagtcct tatcttatct gataaagcag aggaagttcc
    30541 agatatgtat ggttggacaa aggagactgc tgagaccttt gctaagtggc tcaatataga
    30601 acttgaattt caaggttcgg gctctactgt gcagaagcaa gatgttcgtg ctaacacagc
    30661 tatcaaggac attaaaaaaa ttacattaac tttaggagac taatatgttt atttccatca
    30721 gtgctggaat tgtgacattt ttactaactt tagtaggaat tccggccttt atccaatttt
    30781 atagaaaggc gcaaattaca ggccagcaga tgcatgaaga tgtcaaacag catcaggcaa
    30841 aagctgggac tcctacaatg ggaggtttgg ttttcttgat tacttctgtt ttggttgctt
    30901 tctttttcgc cctatttagt agccaattca gcaataatgt gggaatgatt ttgttcatct
    30961 tggtcttgta tggcttggtc ggatttttag atgactttct caaggtcttt cgtaaaatca
    31021 atgaggggct taatcctaag caaaaattag ctcttcagct tctaggtgga attatcttct
    31081 atcttttcta tgagcgtggg ggcgatatcc tgtctgtctt tggttatcca gttcatttgg
    31141 gatttttcta tattttcttc gctcttttct ggctagtcgg tttttcaaac gcagtaaact
    31201 tgacagacgg tgttgacggt ttagctagta tttccgttgt gattagtttg tctgcctatg
    31261 gagttattgc ctatgtgcaa ggtcagatgg atattcttct agtgattctt gccatgattg
    31321 gtggtttgct cggtttcttc atctttaacc ataagcctgc caaggtcttt atgggtgatg
    31381 tgggaagttt ggccctaggt gggatgctgg cagctatctc tatggctctc caccaggaat
    31441 ggactctctt gattatcgga attgtgtatg tttttgaaac aacttctgtt atgatgcaag
    31501 tcagttattt caaactgaca ggtggtaaac gtattttccg tatgacgcct gtacatcacc
    31561 attttgagct tgggggattg tctggtaaag gaaatccttg gagcgagtgg aaggttgact
    31621 tcttcttttg gggagtggga cttctagcaa gtcttctgac cctcgcaatt ttatatttga
    31681 tgtaagaatg gcaccctgat gtttcagggt gtttttgtgt ttaaatacac aatgaaaatc
    31741 aaagaacaaa ctagaaagct aactttaggc tgctcaaaat atagtagatt gaaactagaa
    31801 tagtacacat ctacttctaa aacattttta gaaatcgatt tgactgtcct gaacgatttg
    31861 gcctgttctt atttcatttc actatacaga gttttgagat ggttgataga actgacgaag
    31921 tcagctcaaa acacggtttt gaggttgtag acaaggcgaa gctgatgtgg tttgaagaga
    31981 ttttcgaaga gtgtaaaaaa tattggtcag ttaaagtcaa aatccttgac cttttctgac
    32041 caatgtagta tattatgaac gtaaggtaaa tgaaagcctt actagaacac ttatttaaag
    32101 taaaatagaa agaggtgggt ctatgtttca tctagaaatc ttcagaagta aagatggtct
    32161 actcctgctt gaaaaagaaa aaccagaaat agtacgtagg gtagtggttt agctagtcta
    32221 aattttttaa aacaatggtc aaagatagtc aatatcagta atcataacta agtaaacaaa
    32281 aagaggtaaa gaatatgaat aacaacttta ataattttaa taacatggat gatttattta
    32341 accaattgat gggtggtatg cgaggatata gttctgaaaa tcgccgttac ttaattaatg
    32401 gacgcgaagt cacacctgag gaatttgctc actatcgtgc gactggtcaa ttaccaggaa
    32461 atgcagaaac tgatgtgcaa atgccacaac aggcatcagg tatgaaacaa gacggtgtcc
    32521 ttgcaaaact aggtcgaaac ttgacagcag aagcgcgtga gggtaagttg gatcctgtta
    32581 tcggacgaaa caaggaaatt caagaaacat ctgaaatcct ctcacgccgc accaagaaca
    32641 atcctgtttt ggtcggagat gcaggtgttg gtaagacagc agttgtcgaa gatctagcgc
    32701 aagccattgt gaacggagat gttcctgctg ctatcaagaa caaggaaatt atttctattg
    32761 atatctcagg tcttgaggct ggtactcaat accgtggtag ctttgaagaa aatgtccaaa
    32821 acttagtcaa tgaagtgaaa gaagcaggga atattatcct cttctttgat gaaattcacc
    32881 aaattcttgg tgctggtagc actggtggag acagtggttc taaagggctt gcggatattc
    32941 tcaagccagc tctctctcgt ggagaattga cagtgattgg ggcaacaact caagacgaat
    33001 accgtaacac catcttgaag aatgctgctc ttgctcgtcg tttcaacgaa gtgaaggtca
    33061 atgctccttc agcagagaat acttttaaaa ttcttcaagg cattcgtgac ctctatcaac
    33121 aacaccacaa tgtcatcttg ccagacgaag tcttgaaagc agcggtggat tattctgttc
    33181 aatacattcc tcaacgtagc ttgccagata aggctattga ccttgtcgat gtaacggctg
    33241 ctcacttggc ggctcaacat ccagtaacag atgtgcatgc tgttgaacga gaaatcgaaa
    33301 cggaaaaaga caagcaagaa aaagcagttg aagcagaaga ttttgaagca gctctaaact
    33361 ataaaacacg cattgcagaa ttggaaaaga aaatcgaaaa ccacacagaa gatatgaaag
    33421 tgactgcaag tgtcaacgat gtggctgaat ctgtggaacg aatgacaggt atcccagtat
    33481 cgcaaatggg agattcagat atcgaacgtt tgaaagatat ggctcatcgc ttgcaagaca
    33541 aggtgattgg tcaagataag gccgtagaag ctgtagctcg cgctatccgt cgtaaccgtg
    33601 ctggttttga tgaaggaaat cgcccaatcg gcagcttcct ctttgtaggg tctactgggg
    33661 ttggtaagac ggagcttgct aagcaattgg cactcgatat gtttggaacc caggatgcga
    33721 ttatccgttt ggatatgtct gaatacagtg accgcacagc tgtttctaag ctaattggta
    33781 caacagcagg ctatgtgggt tatgatgaca atagcaatac cttaacagaa cgtgttcgtc
    33841 gcaatccata ctctatcatt ctcttggatg aaattgaaaa ggctgatcct caagttatta
    33901 cccttctcct ccaagttcta gatgatggtc gtttgacaga tggtcaagga aatacagtaa
    33961 acttcaagaa cactgtcatt attgcgacct caaatgctgg atttggctat gaagccaact
    34021 tgacagaaga tgcggataaa ccagaattga tggaccgttt gaaacccttc ttccgtccag
    34081 aattcctcaa ccgctttaat gcagtcatcg agttctcaca attgactaag gaagaccttt
    34141 ctaagattgt agatttgatg ttggctgaag ttaaccaaac cttggctaag aaagacattg
    34201 acttggtagt cagtcaagcg gctaaagatt atatcacaga agaaggttac gacgaagtca
    34261 tgggggttcg tcctctccgt cgcgtggttg aacaagaaat tcgtgataag gtgacagact
    34321 tccacttgga tcatttagat actaaacatc tggaagcaga tatggaagat ggcgttttgg
    34381 ttattcgtga gaaagcctaa gacagaattt tgaggataaa aaagaaggag ccagctgaaa
    34441 aaactggttc cttttgaggt acgacgggca tgtcgtatag taggttgaaa ctagaatagt
    34501 acacatctac ttctaaaaca tttttagaaa tcgatttgac tgtcctgatc gatttgtcct
    34561 gttcttattt cattttacta tatatctgag gtgtaagtcc tcggtgggca cctgttaccg
    34621 gtgaacccaa tagcgattcc caagcctgac tatcgtgagg tagcggatta aaacggtctg
    34681 gggatagacc gttttaagtc tgacgctgga aataagaatc gtcagaggaa gggatagcga
    34741 actcgtggct ctacgaacag gaacgtgata gtaaggcgta tatagcggat aagggggcct
    34801 caaactctaa agtccaaaaa ggtagtcgta acctatatgc gtaaatcacg agagcaactg
    34861 aatttgtcca ttcgaggatg gataaactat ttttcattgg gaaatatgaa aaatatagtc
    34921 actagcatag atgagcgctt gcgtactcgc ctactcgcct acgagtgatt atctggaagc
    34981 aatggaagaa gaaacctaga cgattatgga gattgcttaa gttaggggtt cctaaatgga
    35041 tagcagataa ggtatctggc tggggtgacc actatcaatt agtatctcag aagtcggtac
    35101 ttaaacgtgc tatatcaaaa ccagtccttg agaaacgtgg actggtttcg tgtttggatt
    35161 attaccttga acgacatacg ttaaaagtta gttgaaccgc cgtatgccga acggcacgta
    35221 cggtggtgtg agaggggcta gagattatcc cctactcgat tttaaatcac atgacgttca
    35281 aaggcatcat ctgaaatccc ttgttccaag atgagttttg cccattcttt agcagagaag
    35341 aggctgtggt ccttgtagtt tccgcaagat tcgatggttg tccctgggac atcttcccaa
    35401 gtagtagttt cagcgatttc cttgagcgaa tccttgataa cagctgcgat tttagcactg
    35461 gtgtgacgtc cccacataat catgtggaag cctgtgcggc aaccaaatgg tgaacaatca
    35521 atcatgccgt caatgcgggt acggatgagt ttggctaaga ggtgctcgat agtgtgaagg
    35581 ccggcagtag ggatagagtc ttcgtttggt tgcaccaagc gaatatcata attggagatg
    35641 atgtctcctt ttggtcctgt ttcttcccca atcaagcgaa catagggtgc tttgacaatg
    35701 gtgtggtcaa gttcaaaact ttcgacaata acttcttttg acatggtaaa tcctttcagt
    35761 tttcttctct cattatatca taaaggttgt tcctgagaca gagagaaaac ctctccgagg
    35821 ctagagaggt tgaaatcttt acttacgata taagcggtcg tattggtagt atgggtcaaa
    35881 ggttacgttg atacccagtt tacgaaggac attcttgtct tcatcagtca agatgatggt
    35941 tgagtgggct tcgcttcctt tgaggttgcc gagttcttcc atagcgcggg cagcatcagg
    36001 attttctgta gctgtgatag caagtgcaat caggatttca tttgaatgaa ggcgtggatt
    36061 gcggctaccg agatgatcga ttttaagacc ttggattggc ttaacaactt caggctcgat
    36121 tagttttact tctttagcga tgtcagctga ttttttgatg gcgttgatca aggcagcggc
    36181 tgtaggccca aagagttctg agttcttacc agtgacgatt tccccatttg gcaattcaaa
    36241 ggctagggct ggtccaccag tttcttctgc tttttggcgc gcaacgacag caaccttacg
    36301 gtctgcaggt gtgataccga ggtcgttcat gagcaactca attttcttga cggcagcttc
    36361 gccaactttt tcagctttga agtcaagaac tgtttgatag taacggcgga tgatttcttg
    36421 tttagaagct tcgacagcgg cctcgtcatc tgtaatagcg aaaccaacca tgttgacacc
    36481 catatctgtc ggtgaagcgt atggtgattt tccgagaata cgttccaaca tgcgtttgag
    36541 cactgggaag atttcgatat cacggttgta gttgacagtg gtttctccat aggtttgaag
    36601 atggaagggg tcaatcatgt tgacatcatc aaggtcagct gtggcagctt cataagccaa
    36661 gttaactgga tgatgaaggg gaagattcca aacagggaag gtttcaaatt tagcgtagcc
    36721 agacttgatg ccattgattt ggtcgtggta catattggac atacacgttg ccaattttcc
    36781 agaaccgggt ccaggagcgg ttacgacaat caagttgcga ctggttttga tgtagtcgtt
    36841 tttgcccatg ccttctgggg aaatgatgtg atccatatcc gtcggatatc ctttgattgg
    36901 ataatgaaga taagaatcaa ttccgttttt ctcaagttgg ttgaggaagg catctgcagc
    36961 gggttggcca gcgtattgtg taatgacaac ggaaccaaca aaaatcccta attcattgaa
    37021 tttgtcaatc aaacgaagaa cttcttggtc ataagaaatg cccaagtcgc cacgtgcttt
    37081 ggaatgttca atgttgctag cattaatggc aatcacaacc tcaacctgct ctttcaattc
    37141 ttgcaagagc ttgattttgt tgtcaggttc ataaccagga aggacacgag cagcgtggaa
    37201 atcttctaac attttaccgc caaactctaa gtagagcttg ccgtcaaatt ggttaatgcg
    37261 ctccaaaata tggtcgcgtt gtaaattcaa atactgttca gaactaaaag cttgtttttt
    37321 cattttttta cctctggact ctattataat aaaaaattgg aagttaggaa actacggagc
    37381 taaaaaagaa attaaaaaga ttaagcaaac gcttgcacaa aattttaaaa agtgctatca
    37441 tagactatag attatgaaaa taatgaggta aacagatgca agaaaaatgg tggcataatg
    37501 ccgtagtcta tcaagtctat ccaaagagtt ttatggatag taatggagat ggaattggcg
    37561 atttgccagg aattaccagt aagttggact atctagccaa gctaggaatc acagcaattt
    37621 ggctttctcc cgtttatgac agccctatgg atgataatgg ctacgatatt gctgattatc
    37681 aagcgattgc aacgattttt ggaaccatgg aggatatgga tcaactgatt gcagaagcta
    37741 agaagcgtga tatccgtatc atcatggact tggtggtcaa tcatacctcg gatgagcatg
    37801 cctggtttgt agaggcctgt gaaaatccta atagccctga gcgagactac tatatctggc
    37861 gcgatgaacc caatgaccta gattctatct ttagtgggtc tgcttgggaa tacgatgaaa
    37921 agtcaggtca atactatctc cactttttca gcaagaaaca gccggatctc aactgggaaa
    37981 atgaaaaact tcgccagaaa atttatgaga tgatgaactt ctggattgat aagggtattg
    38041 gtggtttccg tatggatgtt attgacatga ttggcaaaat tcctgacgag aaggtagtca
    38101 ataatggtcc tatgctccat ccctatctca aggaaatgaa tcaggcgacc tttggagata
    38161 aggatctctt gacagtaggg gagacttggg gagcaacgcc agagattgcc aaactctact
    38221 ctgatccaaa ggggcaagaa ttgtctatgg tcttccagtt tgaacatatc ggtcttcagt
    38281 atcaggaagg tcagcctaaa tggcactatc aaaaagagct gaatatcgct aagttaaaag
    38341 aaatcttcaa caaatggcag acagagttag gagttgagga cggctggaat tccctcttct
    38401 ggaacaacca tgacctccct cgtattgtct caatctgggg aaatgaccaa gaataccgcg
    38461 aaaaatctgc caaagccttt gccatcttgc ttcatctcat gagaggaact ccttatatct
    38521 accaaggtga ggaaattggc atgaccaact atccctttga aacgctggat caagtagaag
    38581 atattgaatc tctcaactat gcgcgtgagg ctcttgaaaa aggtgttccg attgaagaaa
    38641 tcatggacag tatccgtgtt attggacgtg acaatgcccg tacccctatg caatgggacg
    38701 agaacaaaaa cgctggtttc tcaacaggtc aaccttggtt ggcggttaat ctaaattacg
    38761 agatgatcaa tgtccaagaa gcgctggcaa atccagattc tattttctat acctatcaga
    38821 aactggtcca aattcgcaag gagaatagct ggctaattcg agctgacttt gaattgcttg
    38881 atacggttga taaggtcttt gcttatatac gtaaggatgg cgaccgtcgc ttcctagttg
    38941 tggctaactt gtccaatgaa gagcaagact tgacagtaga aggaaaagtc aaatctgtct
    39001 tgattgaaaa caccctagct caagaagtct ttgaaaaaca aatcttagtt ccatgggatg
    39061 ctttctgtgt ggaattacta taaatatttt ttgcagaaaa atttaaaatt gaaatcatat
    39121 aaaaacaagg gatgattgta taaaaggtag aaatcctttg ttttttataa ccaaggttta
    39181 taaactttca ttctcgaaat tcaattaact ttacaaattc ccactattaa ggagaaagaa
    39241 gatgaacata aagaagcgtg tccttagtgc aggcctgact tttgcatctg ctttgctttt
    39301 agctgcttgc ggctaatcag gttcagatac aaaaacttac tcatcaacct ttagtggaaa
    39361 tccaactaca tttaactatc tattagacta ttacgctgat aatacagtca attgaaacaa
    39421 gaacaagaca aaagaacctc gtaaaaggta ttgtaacttg gtaatacctt tttgaggtgc
    39481 tttttgatat gagcccatgt tttctcaata ggattgtact caggtgagta gggaggaaga
    39541 ggtaaaagtt tatgcccaaa ctcctcgcat aaaagttcta acttacccat tctatggaat
    39601 cttgcattat ccataataat aaccgatggt gtggttaatg ttggtaagag aaacttctga
    39661 aaccaagctt caaaaaagtc gctcgtcatc gtctcttcgt aagtcattgg aacgattaat
    39721 tcaccatttg ttagacctgc aaccaaagaa attctctgat atcttcttcc agatactttg
    39781 cctcttctta actgaccttt taatgagcga ccatattctc gataaaaata agtatcgaat
    39841 cctgtttcgt caatctaaac aggtgctagg tgctttaaac tattaaaatt cttaagaaat
    39901 aaggctactt tttctgggtc ttgttcatag taggtgtggt tcttttttcg agtgtagccc
    39961 atagctttga gcgcatagtg gatggtagtt ggatgacagc caaattcaga agctatttca
    40021 gtcaaataag cgtctggatt gtcagtaaga tagtttttaa gtctatctct atcaactttt
    40081 cttggttttg ttccttttac ttggtggttt agctctcctg ttttctcttt tagctttaac
    40141 cagccataaa tggtattacg tgagatttgg aaaacgtgtg acgcttctgt tatactacct
    40201 gttcgctcac aataagagag aactttttta cgaaaatcta ttgaatatgc cataaaaaga
    40261 ttataccaca ttgtgtacta tattagattg aaactagaat agtacacatc tgcttctaaa
    40321 acattgttag aaatcgattt gactgtcctt atttcatttt attatatttt tggttcgcgg
    40381 gaagtctact aagatactta aagatgcaga tagtgaaaat aaaggtgtag acattaccgt
    40441 aaaaaagtga tataatcgta tgatgttcaa tgtataggtg ttaatcatga gtagacgttt
    40501 taaaaaatca cgttcacaga aagtgaagcg aagtgttaat atagttttgc tgactattta
    40561 tttattgtta gtttgttttt tattgttctt aatctttaag tacaatatcc ttgcttttag
    40621 atatcttaat ctagtggtaa ctgcgttagt cctactagtt gccttggtag ggctactctt
    40681 gattatctat aaaaaagctg aaaaatttac tatttttctg ttgctgttct ctatccttgt
    40741 cagctctgtg tcgctctttg cagtacagca gtttgttgga ctgaccaatc gtttaaatgc
    40801 gacttctaat tactcagaat attcaatcag tgtcgctgtt ttagcagata gtgatatcga
    40861 aaatgttacg caactgacga gtgtgacagc accgactggg actgataatg aaaatattca
    40921 aaaactacta gctgatatca agtcaagtca gaataccgat ttgacggtca accagagttc
    40981 gtcttacttg gcagcttaca agagtttgat tgcaggggag actaaggcca ttgtcctaaa
    41041 tagtgtcttt gaaaatatca tcgagtcaga gtatccagac tacgcatcga agataaaaaa
    41101 gatttatacc aagggattca ctaaaaaagt agaagctcct aagacgtcta aaaatcagtc
    41161 tttcaatatc tatgttagtg gaattgacac ctatggccct attagttcgg tgtcgcgatc
    41221 agatgtcaat atcctgatga ctgtcaatcg agataccaag aaaatcctct tgaccacaac
    41281 gccacgtgat gcctatgtac caatcgcaga tggtggaaat aatcaaaaag ataaattaac
    41341 ccatgcgggc atttatggag ttgattcgtc cattcacacc ttagaaaatc tctatggagt
    41401 ggatatcaat tactatgtgc gattgaactt cacttcgttt ttgaaattga ttgatttgtt
    41461 gggtggaatt gatgtttata atgatcaaga atttactgcc catacgaatg gaaagtatta
    41521 ccctgcaggc aatgttcatc ttgattcaga acaggctctc ggttttgttc gtgagcgcta
    41581 ctcactagca gatggcgatc gtgaccgcgg gcgcaatcaa caaaaggtga ttgtagctat
    41641 ccttcaaaaa ttaacgtcaa ccgaagcact gaaaaattat agcacgatca ttaatagctt
    41701 gcaagattct atccaaacaa atatgccact tgagactatg ataaatttgg tcaatgctca
    41761 gttagaaagt ggagggaatt ataaagtaaa ttctcaagat ttaaaaggta caggtcggac
    41821 ggatcttcct tcttatgcaa tgccagacag taacctctat gtgatggaaa tagatgatag
    41881 tagtttagct gtagttaaag cagctataca ggatgtgatg gagggtagat gaaatgatag
    41941 acatccattc gcatatcgtt tttgatgtag atgacggtcc caagtcaaga gaggaaagca
    42001 aggctctctt gacagaatcc tacaggcagg gggtgcgaac cattgtctct acctctcacc
    42061 gtcgcaaggg catgtttgaa actccggaag agaagatagc agaaaacttt cttcaggttc
    42121 gggaaatagc taaggaagtg gcgagtgact tggtcattgc ttacggggct gaaatttact
    42181 acacaccaga tgttctggat aagctggaaa aaaagcggat tccgaccctc aatgatagtc
    42241 gttatgcctt gatagagttc agtatgaaca ctccttatcg cgatattcat agcgccttga
    42301 gcaagatctt gatgttggga attactccag tcattgccca cattgagcgc tatgatgctc
    42361 ttgaaaataa tgaaaaacgc gttcgagaac tgattgatag gggctgttac acgcaagtaa
    42421 atagttcaca tgtcctcaaa cccaaacttt ttggcgaacg ttataaattc atgaaaaaaa
    42481 gagctcagta ttttttagag caggatttgg ttcatatcat tgcaagtgat atgcacaatc
    42541 tagacggtag acctcctcat atggcagaag catatgacct tgttacccaa aaatacggag
    42601 aagcgaaggc tcaggaactt tttatagaca atcctcgaaa aattgtaatg gatcaactaa
    42661 tttaggagaa atgatgaaag aacaaaacac gatagaaatc gatgtatttc aattacttaa
    42721 aaccttgtgg aaacgcaagc taatgatttt aatagtggca cttgtgacag gtgcgggggc
    42781 ttttgcatat agcactttta ttgttaagcc agaatatacg agtaccacgc gaatttacgt
    42841 agtgaatcgc aatcaaggag acaagccggg gctgacaaat caggatttgc aggcaggatc
    42901 ttatctggta aaagactacc gtgagattat cctttcgcag gatgcattgg aaaaagtagc
    42961 gacaaatttg aagttggata tgccagcaaa aacgttagcc agcaaagttc aagtgactgt
    43021 accagctgac actcgtatcg tctcaatctc tgtcaaggat aaacagccag aggaagccag
    43081 tcgtatcgct aattctctac gagaagttgc tgcagaaaag atcgtcgctg taacgcgagt
    43141 atctgatgta acgacacttg aagaagcgcg accagctacg actccctctt ctccaaatgt
    43201 tcgacgcaat tccttgtttg gttttcttgg aggagcagtc gtaacagtaa ttgctgttct
    43261 tttgattgag ttgctggaca cccgtgtgaa acgtcctgaa gatgttgaag atgtactgaa
    43321 aattccactt ttagggctcg ttccagattt tgacaaaata aaataggagg aagttatgcc
    43381 aacattagaa atctcacagg caaaattgga ttctgtaaaa aaggcagagg aatattataa
    43441 cgctttgtgc acgaacctac agttaagtgg agatggtttg aaagtatttt ctatcacttc
    43501 tgtgaaaata ggagaaggaa aatcaacgac ttccgccaat atcgcttggg cttttgcgcg
    43561 tgcaggttac aaaacgctgc tgattgatgg agatattcgc aattctgtta tgttaggtgt
    43621 ctttaaagca aggaataaga ttacaggcct gacagaattt ttatcaggaa ctacagactt
    43681 atcacaaggg ctttgtgata ccaatatcga aaatctcttt gtaattcagg ctggctctgt
    43741 gtcaccgaat ccgacagctc ttcttcaaag taagaatttc actacaatgc ttgaaacctt
    43801 gcgtaaatat tttgactaca tcattgtaga tactgctcct gttggtgtcg tgattgatgc
    43861 ggctattatt acgcgaaact gcgatgcttc tattttagtg acggaggcag gtgaaataaa
    43921 tcgacgggat attcaaaaag caaaagaaca gttggaacac acagggaagc cgtttttggg
    43981 agttgtgttg aataaattcg atacttcagt agacaaatac ggttcttatg gaaattatgg
    44041 agattacggg aaaaataaaa aataggtcgg gggatagaga tgaatggaaa aatagtaaag
    44101 tcttcattgg ccataatcca gagttttctt gttattttat tgacttatct acttagtgct
    44161 gtgagagaag cggagattgt ttcaacaaca gctattgcac tttatatcct ccattatttt
    44221 gtcttttata tcagtgatta tggacaggat ttctttaaaa ggggatattt gattgaactt
    44281 gtccagacat tgaaatatat cctattcttt gcactagcga ttagtatttc taattttttc
    44341 ttagaggatc gatttagtat ttccagacga ggcatgattt acttcctcac attacatgct
    44401 ctcttagtct atgtgctaaa cctatttatc aagtggtatt ggaagcgggc ttatcccaac
    44461 tttaaaggaa gtaagaagat tctcctactt acagcaactt ctcgtgtcga aaaggtactg
    44521 gatagattaa tagaatcaaa tgaggttgtt ggggagttgg tagccgtcag tgtcttagat
    44581 aaaccagatt ttcagcatga ttgtttaaag gtagtagcag agggggagat agtaaacttt
    44641 gcgactcatg aggtggtcga tgaagtcttt atcaatcttc caagtgaaaa atacaatatt
    44701 ggagagcttg tctctcagtt tgaaacgatg ggaattgatg taacagtcaa tctaaatgct
    44761 tttgatcgta gtttagcacg taacaagcaa attcgtgaga tggcaggatt aaacgttgtg
    44821 actttttcta caacatttta taagactagt catgtaattg ctaagcggat tattgatatc
    44881 gtgggtgcat tggtagggct gatactatgt ggtttagtca gtattgtact ggttcctttg
    44941 attcgaaagg atgggggctc tgctattttt gctcagacgc gtataggaaa aaatggtcgt
    45001 cagttcactt tttataagtt tcgctctatg tgtgtagatg ccgaggcgaa aaaaagagaa
    45061 ctcatggaac aaaataccat gcagggtgga atgtttaagg tggacgatga tcctcgtatc
    45121 acgaaaattg gtcgttttat acggaagact agcttggacg agctaccaca gttttacaat
    45181 gttctaaagg gagatatgag cttggtaggt acacgaccac caacagtgga cgagtatgag
    45241 cactataccc cagaacaaaa acgccgacta agttttaaac ctggcataac aggtttatgg
    45301 caggtcagcg gacgaagtga aatcaagaat ttcgatgaag ttgtcaaatt agatgtggcc
    45361 tatattgatg gttggacaat ctggaaagat attgaaattt tattgaaaac agttaaagtt
    45421 gtatttatga gagatggagc gaagtaattt ctgtatatcc atcatattag gagagaaatg
    45481 aaaaagtcag tttatatcat tggttcaaaa ggaattcctg ctaagtatgg aggatttgaa
    45541 acttttgttg aaaaattaac agccttccaa caagataagg ctatccaata ttatgtggct
    45601 tgtatgcgtg aaaactctgc aaaatcaggg actactgagg atgtttttga acataatggt
    45661 gctatctgtt ataacgtcga tgttcctaat attggtccag cgcgagctat agcgtatgat
    45721 atcgctgcaa ttaacagagc tattgaaatt gccaaagaaa ataaggatga agatccaatc
    45781 ttctatattt tagcttgtcg aatcggtccg ttcatccatg gaattaagaa aaaaatccag
    45841 gcgataggtg ggactcttct agttaatcca gatggtcacg agtggttacg agctaagtgg
    45901 agcgctccag ttcgtcgtta ttggaaaatt tccgaaggtc ttatggtcaa acatgcagct
    45961 cttttagtgt gtgatagtaa gaatattgaa aaatatatcc aagaagatta tatacagtat
    46021 caacctaaga caacctatat cgcttatggg accgatacaa cacgctctac attaaagagt
    46081 agtgatgaaa aagtacgttc ttggttcaaa gagaagaatg tttctgagaa cgagtattat
    46141 ctagttgtag gacgttttgt accagaaaac aactatgaat ctatgattcg tggctttttg
    46201 gcgtctaact ctaagaagga ctttgtctta atcacaaatg tagaacagaa taaattttat
    46261 aatcagttgt tggcaaaaac tggttttgat aaggacccac gagtaaaatt tgtaggtaca
    46321 gtctatgagc aagagcttct gaagtatatt cgagaaaatg cttttgctta cttccacgga
    46381 catgaagttg gtggaacaaa tccttcgtta cttgaagcat tagcatccac aaagttaaac
    46441 ttactactcg atgttggttt taaccgcgaa gttggggagc aaagtgcgat ctattggaaa
    46501 aaagatgagc tttcccaagt aatcgagaaa gttgaacaat ttgatgcaaa aatgattgat
    46561 gagttagata gacaatcaaa tcagagaatt gcggattatt tcacttggga aaagattgtc
    46621 acagactacg agaaagtatt taaaggttag agttaatggc agtaaaatta aaatatgata
    46681 aatcagatat tacattttta gatgatgacg gattgaaaaa ataccaatca gaattgttga
    46741 aaatgatatc agatgtgttt actttttttg atgaaaatgg gatagcgtat tctttaagtg
    46801 gaggtagcat tttaggggca atccgtcata aagggtttat cccttgggat gatgatgttg
    46861 atatcaatat tccgagggaa agttacgata aactattttc tctatttgag tcagacaata
    46921 gtcttagtcg taaatattat cttcaatcag caaaaagtca tccagaattg ggacttcatg
    46981 tttctcaaat tagaaaaaaa ggaacagttg ctcgtaggaa atacgatcat tcggctgagg
    47041 agtgcggtat ttctatagat ctttacattg tagaaaatgt atacaataat ccagtgaaac
    47101 gattctttca aggttataca agtatgtttt tgacttttgc cttggcttct gtacgagaaa
    47161 caaagaatca tgccctgatg aaggaaatgt ttagactgga aggtagaaaa ctaaattatt
    47221 caactggaaa gttaatggtt ggttggtttt ttggaattat tccaattgaa aagtggttga
    47281 attggcttga taaatgtaat tcaagctgta aggattcaca tacaaaatat gtatccatac
    47341 ctacaggaag gaaacacttt agacgcgaga cttacttgcg agagaatatg aatatttata
    47401 aaaaggttcc ttttgaaact ttgatggtga atgttcctgt ttggtcagaa gagtatttag
    47461 aaatgtttta cggtaaagat tatatggtcg ttcctcctgt tgataagaga gagcaacatt
    47521 tgtttttaga gttgagttac ggagaagaat aaatgaaagt agcgatttta acagcttcag
    47581 gcattggaag ccgaattggt caagatatcc ctaaacaatt tattcatgtt gaaaataagc
    47641 cagtcattat ctataccctt gaaaaatttc aaaatcatcc agaaattgat gaaatctgta
    47701 ttgtcatttt aaaaggctgg gagcagatgg ttaaagctta tgcggaacaa tttggaatta
    47761 caaagttaaa gatgataact ttgggaggga agtctggtca agaatcaatt tataacggtc
    47821 ttagagaagt taagaaagct catcccaatg aggatgtgac tgtattaatt catgacggga
    47881 atcgtccttt agtgagcaat gatattatta gcaacgcttt agcaacatac caacagtttg
    47941 gaaatgctgt tgcagcgata ccaacgactg aggtagtatt tgttttagaa aacccacaat
    48001 caacttcgtc cacagaagca ttgaatcgtg acttgttgag acgaacacaa actcctcatg
    48061 tttaccatct tgataatatt ctatcgcttc atgaaaaagc attagaaaat gggataactg
    48121 atgttgcagc atcatgccaa ctcatgcaac tatttggtga aaagagttat ttctctcttg
    48181 gaacagagaa aaacttgaaa attacgactg tagaagatct cgatattttt aaagccttat
    48241 tgagttcaac tcgagataag tggattaaat agtaatgaca ttattattaa ataaaatata
    48301 tagacaagat gtagaatctt ctctagaaac aatcgtcaat attaatgact tgaatggcaa
    48361 gtcaatttta attactggag cttctggttt gattggttca agtgtcattg accagttatt
    48421 attgctaaat gaaataagga attttaacat taaaattttt tgttgtggaa gaaagtttga
    48481 atcattagaa aaacgatttg gtagacaaac agagtattta cgctttgtgt tctatgatgc
    48541 gactaaagca cctcactttg attttgcagt ggactacatt attcatgctg ctagtcctgc
    48601 tagtcctgaa ttatatgtta atcagcctgt agaaacaatg gctagtaatt ttctgggcat
    48661 gtataatcta ctgacatacg caaaaaatgc taaagtttca aatctagttt atatctcatc
    48721 tagtgaagtt tatggaattg gcaccagtaa taatcctttg gaggagagtt atattggaac
    48781 tgttgatcat cttagcgtac gttcttcgta tgctagttct aagagagcta ctgaaacact
    48841 ttgtgctagt tttgcttccg agtacgggat caaaatcatg atagtaagac caggacacat
    48901 atatggccct agtgccaaga aaagtgataa tcgtgtatca tcatttttta tgatagaagc
    48961 gattgctggt cgtgatatca ttatgaaaag tacagggagt cagttgagat cgtattgtta
    49021 tagtttggat tgtgctagtg ctattttaag tgttctcctt tcgggtgaat cagggcaagc
    49081 ttataatgtt tccaatccaa attcaattat tactataaaa cagatggctg aaattatttc
    49141 tcgagttggc aatgtgaaac tcaccatgga tattcctagt gaccaagata ttaagcagac
    49201 taatcctatg cagaacgccg gtctaagcag tgataagcta gaagaattag gatggcaagg
    49261 acattttaca gctgaggaag gttttcaaca tacatttaat attttaaagg agttatccag
    49321 tgaaaagtga tagcatttta atcatgatgg ctacctacaa cggagaagaa tttcttcgtg
    49381 aacagttgga tagtatcctt tgtcaaacca tctcaacttg ggaactactg attcgtgatg
    49441 ataattccaa tgatcaaaca agagcaatta ttaaagaata cgaagaaaaa gatagacgga
    49501 ttaaattgat tgaaaacata tcggaatatc atggagccta ttataatttt tgggggttat
    49561 taaacgatgt tagacaaaac gaaaatccat ttgatttcta tatgtttgct gatcaggatg
    49621 atatctggga tgtggacaaa ttagaacgtt taattagtta ttactataga aaagttaaaa
    49681 cagaagaacc ggtattgatt tacgctgata tgcggattat agatgctaac ggtaaagtga
    49741 tagctaatag tatggatcaa ttgatgggaa ttcggtatac caatcctatc tcaactttta
    49801 tggctcataa ggtttatgga tgtaatacgt tatttaatca tgaattattt gaaatcttac
    49861 ccctccttcc atgttatgct ccagaattag ccttcctatc acatgataac tttacaacaa
    49921 aaattgctgc attgaaaggg catgtgtatt tttatgatga gcctactatg agttatagac
    49981 gatatgggca taatgttaca agtaaacatg agtataactt tacattgaag cgaatcttaa
    50041 aacgtatctc gaaaattgat gaattagcta aagatcatgc cttgacttac aagcagacct
    50101 tagtcgctac aaatttgtta catcaacaaa caagtattga cactgaactc ctatttttag
    50161 ataaggtaga aaaaataatc aaaaaaggtg gattaaatgc tgtaaaaatg gtttggaaag
    50221 aaaaaattga ttggggaaat cgtgttaaga ctataagcag gtctctgatt ttagcgtcaa
    50281 aattatataa gaaatattta taaaagatat ttaaatacaa aaactctgtt ttttatatgt
    50341 cacaactaag aacaactttc aaattaaagt gatttaataa aaatttaaag gaataataat
    50401 gtcaaaaaaa gaaatcaaac caatttgttt ttacttaccg caatttcatc ccatcccaga
    50461 gaatgatgag gctcatggag aaggatttac tgaatggact aatgtaaaga aagcctatcc
    50521 actttatgac gggcataatc aaccacgagt tccatacaat aataattatt atagtctatt
    50581 agataaagaa gtcttgattc aacaggcaga attggctaaa aagtatggaa tttacggatt
    50641 ttgttattat cattactggt ttaaaaatgg taaaaaacta ttagaaaaac cagtagaaat
    50701 gatgctagaa aacaaggatg ttgatatacc tttttgtttg agttgggcaa atgaaaactg
    50761 gactaggcgt tgggatggag ggaatcatga aatcattgtt gaacaaaact atgacgactt
    50821 aaatgatttg gagaaacatg tggattatct gtgtcaattt tttaaagatg accgatatat
    50881 taagattgac gggaaaccgc tcttgttgat ttataagcca gagctaattc caaatttagt
    50941 aaagacaatg gaaattatca gaaatcgtgc tcgaaagaat ggctttaagg atttagagat
    51001 tattgtacag tttccaaagt tcttgttaaa taatcatcag cttgagttat ttgatggatt
    51061 tgcagaattc caaccgattt ttgagattca tatggaaaag cacgagagaa aaaatccagt
    51121 ttaccgatct gtgaaatctt ttctacttaa agttggggct cgaaatttag tacaaaaact
    51181 tgagattaga ggtgaaaaaa aggtgcataa taaagctctc ttagatagga gagattatga
    51241 tgcaacatgg actaatatta ttaatcgccc gattaaggat aataagatga ttgctggtgc
    51301 ttttgttgat tgggataata ccgctagaaa taagaatggg cgagttttcg atggagctaa
    51361 tccagaaaaa tttgaaggtt acatgcgaca actaattgaa aaaattcaaa aagagtatca
    51421 atcagaaatt gtttttataa acgcatggaa tgaatgggcg gaaggagcat accttgagcc
    51481 tgataaaaaa catggatatg gctatctaga ggccttgaag acagttatag acgagacaag
    51541 gtaggaaaag ctatgaatat tcgttataaa cttcaatttc gacgatgtaa aaaagagtgg
    51601 cgaaaaaaga accttcataa tgaaacaacg cctcgaacta tgttcccaat agaattggtt
    51661 tcagtagggg aaaaaaccta tggtggtttg tatgttttgt ccttcaataa agagtcaaaa
    51721 ttaaaaattg gaaatttctg ctccattgca ccagaggttg cttttattct cagtgctgat
    51781 cattatatca atcagttctc tacgtatccg ttcaaatcta aagtatttga tcaaggtgac
    51841 gaaggggttt cgaaagggga tattatcatt gaggatgatg tatggattgg ttttcgttct
    51901 acaattcttt cgggagttac aattggtcag ggagctattg ttggagcagg atctgtggta
    51961 actaaatcag ttcctcctta cgctattgta ggtggagttc cagcaaaagt gatttcttat
    52021 cgattcgaaa cagagattcg tgaagaaatg aaaaaaattg acttttcgga gttcaaatta
    52081 gagaaattta aaaaggttac ggaagagttg tatcagccaa tttcgtcaat aagacaaatt
    52141 aataacataa atgaaaaatt gaaatagagt cggaggagtt gcatgtttcg ttttcagaat
    52201 gggacagtat caataaacat gagaggattt ctgatgttca ttgcaatgct catttactgt
    52261 caaccagaat atttgaaatc aattccagtt acgggacgta tttatagaga tttcatgcat
    52321 gtaggaatta tgtttgtgac gttttcgtac atttcaaaaa aacggtttcc aaaatttttt
    52381 gtatggctag ttcttctata taactcttgg cttacttttt taactattca aaataacgga
    52441 gatataaaaa gggcgatatt tcaactcttg ctgtttacag gctttgtatc tatgatagac
    52501 atattctctg aaaatatgca ggatttaata gaatcattgt tattcacttt tgagatggtt
    52561 gtgtatctaa actttgtcac tgtactattt ttcccagatg gttttttcca tcgtgtaaat
    52621 gatgcatacg gaataacaaa agaatggttt ttgggagctg ataataattt tctatttacc
    52681 ttgtttcctg caacacttat agctttattg tataagaatt taggtggagt aaaaatcaga
    52741 gcatattcat tgatttcaat cattttagct actcaattga ttaaaggatc gggaacggca
    52801 aaggttggga ttgttgcgtt gatactattt gatttagtac ctcttgtcaa atacatactt
    52861 accccttaca gaggtttggt tatatcgtct acgttgagtt ttctaattgt agttactggt
    52921 aaagtggaat ttttaagagg gattgttgaa ggtattctag aaaaagattt gacgttcact
    52981 tcccgaactt ttatttggaa taacgctatg tcagcaattc gagagaagcc atttggttac
    53041 ggaatgatga tgttcactga taacgtccat atattaggga attttcctgg ttggatttgg
    53101 aaaggagcca cccatgctca taataacttt cttcaagttt tatttcaggg aggatattta
    53161 ggatttggtg tttatttttt aataattcta aagggagcaa tgctatgtcg caaatattgg
    53221 gatgatagaa atgcgagact actcttttat ggacttttta tttttacaat aatatctata
    53281 acagaaaact tagagatgca gatgatgtat ttggtgatta tactaccgtt tttattgcct
    53341 aatatattgg ttaaagaaag gggaaaaaat gaatagaggt attttggtat caataattgt
    53401 tcctatctat aatcaagaag aatatttaaa tatttcttta cctactttga agcagcagac
    53461 gtataagaat ttagaattta tcttagtaga tgatggttcg acagatagta gcaaacagat
    53521 aattaacgag tatcttaatg cagacagtag atttaaatat gtgtataaag aaaatggagg
    53581 cttagtatct gctacaattg caggggtgaa agaggcttcg ggagactatc ttgctttttt
    53641 agatccagat gataagttag gattggacta tatagaaaac ttcatcaaag aattggattg
    53701 tgactatgat ttcatagcgg caggtttttt ctatgacgat ggtaagttaa ttaaaccata
    53761 ttacttgaaa gagaattcag tcttgactca atcagatata aagaagttaa gaacggactt
    53821 tttgtcggat acaggcgggt tagctatttc aaatcgtgtt tttatctcaa gatggaataa
    53881 attatataag acaaatcttg taaaagaaat tgtcaaagag tttcgtaaat ttgaaatggt
    53941 tagcttgggt gaagatacta tttttacatt tttacttcta caaaaggctc aattagggaa
    54001 aaccattgcc ctatctaatt cttattttta taatgtaggt aatcctaact caatgatgaa
    54061 ggaaggagct attataaact atgttgaaaa atgtaggata gcatatcaga gtcttactaa
    54121 gctacttata gaaagaaatt tgactaatga aatgagttgt caactttact atttcttagt
    54181 gctttctttc ttcaatcgct tagagaaaga agatatggag tctttcaaaa aaatatatga
    54241 tattttgaaa agggattcac tatttttaaa aattctattt tcacttcaaa agaaagcaaa
    54301 taaaaaacaa aaacttcagc tattgatgat tcgtctatgc aaaagtggga aacagtactt
    54361 aaacattaaa agagtagttt caaaactcca tgcaaaactt aagttgttct gtcagaattt
    54421 taagttagtg aggaaacaga tttttaggaa gaatctagtt caattagctt attttttaaa
    54481 gttccaacaa caacggaaaa ttggttttga tgatatgaag gtgtttttac cgataattga
    54541 agaaagatta aaagaagatt tattggaatt tcaaaaatta gaagtattac cttcaacaat
    54601 tgaaaagaat atttttgttt tttggtggga tggctttgat gagatacctg aaatagtaag
    54661 tatttgctta gcatctttaa aaaagaatta tcca
//
LOCUS       DAVKQL010000004        51153 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVKQL010000004 DAVKQL010000000
VERSION     DAVKQL010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 51153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51153
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.004"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            167..955
                     /gene="codY"
                     /locus_tag="VW561_000168"
     CDS             167..955
                     /gene="codY"
                     /locus_tag="VW561_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEU9942509.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            955..1530
                     /locus_tag="VW561_000169"
     CDS             955..1530
                     /locus_tag="VW561_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU9942510.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            1612..1697
                     /locus_tag="VW561_000170"
     tRNA            1612..1697
                     /locus_tag="VW561_000170"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1646..1648,aa:Leu,seq:aag)
     gene            1980..3110
                     /gene="ugpC"
                     /locus_tag="VW561_000171"
     CDS             1980..3110
                     /gene="ugpC"
                     /locus_tag="VW561_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEU9942511.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(3257..3403)
                     /locus_tag="VW561_000172"
     CDS             complement(3257..3403)
                     /locus_tag="VW561_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942512.1"
                     /translation="MEIKEQTRKLAVSCSKYIFEVADKTDEISNHTYGKATLTWFEEI
                     FEEY"
     gene            3611..4369
                     /locus_tag="VW561_000173"
     CDS             3611..4369
                     /locus_tag="VW561_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU9942513.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            4456..4968
                     /locus_tag="VW561_000174"
     CDS             4456..4968
                     /locus_tag="VW561_000174"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEU9942514.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            5150..6094
                     /locus_tag="VW561_000175"
     CDS             5150..6094
                     /locus_tag="VW561_000175"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEU9942515.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWINHAVYQETPFDWRKIEDDASAYGYL"
     gene            6103..6780
                     /locus_tag="VW561_000176"
     CDS             6103..6780
                     /locus_tag="VW561_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEU9942516.1"
                     /translation="MTYLDAFKSGILVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            6879..7637
                     /locus_tag="VW561_000177"
     CDS             6879..7637
                     /locus_tag="VW561_000177"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU9942517.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            7675..9180
                     /gene="lytC"
                     /locus_tag="VW561_000178"
     CDS             7675..9180
                     /gene="lytC"
                     /locus_tag="VW561_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HEU9942518.1"
                     /translation="MSVTFFIGEERLKIKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSAEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMIQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            9677..10195
                     /locus_tag="VW561_000179"
     CDS             9677..10195
                     /locus_tag="VW561_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEU9942519.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            10325..10831
                     /locus_tag="VW561_000180"
     CDS             10325..10831
                     /locus_tag="VW561_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEU9942520.1"
                     /translation="MTKKIVAIWAQDEEGVIGKDNRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            10831..11001
                     /locus_tag="VW561_000181"
     CDS             10831..11001
                     /locus_tag="VW561_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942521.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            11033..12265
                     /gene="clpX"
                     /locus_tag="VW561_000182"
     CDS             11033..12265
                     /gene="clpX"
                     /locus_tag="VW561_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEU9942522.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            12274..12861
                     /locus_tag="VW561_000183"
     CDS             12274..12861
                     /locus_tag="VW561_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEU9942523.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            12872..13252
                     /locus_tag="VW561_000184"
     CDS             12872..13252
                     /locus_tag="VW561_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU9942524.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            13304..14194
                     /gene="rapZ"
                     /locus_tag="VW561_000185"
     CDS             13304..14194
                     /gene="rapZ"
                     /locus_tag="VW561_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEU9942525.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLVQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            14191..15168
                     /locus_tag="VW561_000186"
     CDS             14191..15168
                     /locus_tag="VW561_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEU9942526.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDFRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            15165..16076
                     /gene="whiA"
                     /locus_tag="VW561_000187"
     CDS             15165..16076
                     /gene="whiA"
                     /locus_tag="VW561_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEU9942527.1"
                     /translation="MSFTVAVKEEILGQHHLSWHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(16110..17078)
                     /locus_tag="VW561_000188"
     CDS             complement(16110..17078)
                     /locus_tag="VW561_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU9942528.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPGQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            17214..18071
                     /locus_tag="VW561_000189"
     CDS             17214..18071
                     /locus_tag="VW561_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEU9942529.1"
                     /translation="MNFQQLSNLQYWTSLFASPWAIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERW
                     LGGWKHEKK"
     gene            18058..18837
                     /locus_tag="VW561_000190"
     CDS             18058..18837
                     /locus_tag="VW561_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEU9942530.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSISARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            18861..20213
                     /locus_tag="VW561_000191"
     CDS             18861..20213
                     /locus_tag="VW561_000191"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU9942531.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            20287..20661
                     /locus_tag="VW561_000192"
     CDS             20287..20661
                     /locus_tag="VW561_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEU9942532.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWESENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            20663..21511
                     /locus_tag="VW561_000193"
     CDS             20663..21511
                     /locus_tag="VW561_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9942533.1"
                     /translation="MKLAVFTDSSAYLSEETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            21788..22555
                     /locus_tag="VW561_000194"
     CDS             21788..22555
                     /locus_tag="VW561_000194"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEU9942534.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            22567..23751
                     /locus_tag="VW561_000195"
     CDS             22567..23751
                     /locus_tag="VW561_000195"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEU9942535.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNLQAITEKYQ
                     SLTIHDKKEIQINGDILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            23748..25619
                     /locus_tag="VW561_000196"
     CDS             23748..25619
                     /locus_tag="VW561_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9942536.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELERAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQDVSFAYENKPILQD
                     FNLLVQVKDRIGIVGDNGVGKSTLLNLIAGSLESTKGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(25762..26946)
                     /gene="mntE"
                     /locus_tag="VW561_000197"
     CDS             complement(25762..26946)
                     /gene="mntE"
                     /locus_tag="VW561_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEU9942537.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            27250..29946
                     /locus_tag="VW561_000198"
     CDS             27250..29946
                     /locus_tag="VW561_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEU9942538.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LLEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEDHVLTGAELNELSDEDFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            29959..30750
                     /gene="yghU"
                     /locus_tag="VW561_000199"
     CDS             29959..30750
                     /gene="yghU"
                     /locus_tag="VW561_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEU9942539.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTVGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTEDVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            30866..31276
                     /locus_tag="VW561_000200"
     CDS             30866..31276
                     /locus_tag="VW561_000200"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU9942540.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANHDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRV
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            31288..32883
                     /locus_tag="VW561_000201"
     CDS             31288..32883
                     /locus_tag="VW561_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942541.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPRSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTK
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESINGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKLR"
     gene            32950..33114
                     /locus_tag="VW561_000202"
     CDS             32950..33114
                     /locus_tag="VW561_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEU9942542.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            33193..33921
                     /gene="yaaA"
                     /locus_tag="VW561_000203"
     CDS             33193..33921
                     /gene="yaaA"
                     /locus_tag="VW561_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEU9942543.1"
                     /translation="MKILIPTAKEMNTDLPSIEAISLKPESQAVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTTQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(34198..34746)
                     /locus_tag="VW561_000204"
     CDS             complement(34198..34746)
                     /locus_tag="VW561_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9942544.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIK"
     gene            35067..35555
                     /locus_tag="VW561_000205"
     CDS             35067..35555
                     /locus_tag="VW561_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEU9942545.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQVDQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKIIFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            35552..36739
                     /locus_tag="VW561_000206"
     CDS             35552..36739
                     /locus_tag="VW561_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9942546.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTAILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            36732..37097
                     /locus_tag="VW561_000207"
     CDS             36732..37097
                     /locus_tag="VW561_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9942547.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGAPGYRSERLIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            37113..38459
                     /gene="asnS"
                     /locus_tag="VW561_000208"
     CDS             37113..38459
                     /gene="asnS"
                     /locus_tag="VW561_000208"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEU9942548.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            38612..38902
                     /locus_tag="VW561_000209"
     CDS             38612..38902
                     /locus_tag="VW561_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU9942549.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            38914..39384
                     /gene="ssbA"
                     /locus_tag="VW561_000210"
     CDS             38914..39384
                     /gene="ssbA"
                     /locus_tag="VW561_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEU9942550.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            39416..39655
                     /locus_tag="VW561_000211"
     CDS             39416..39655
                     /locus_tag="VW561_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEU9942551.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            39950..41350
                     /locus_tag="VW561_000212"
     CDS             39950..41350
                     /locus_tag="VW561_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEU9942552.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            41352..41711
                     /locus_tag="VW561_000213"
     CDS             41352..41711
                     /locus_tag="VW561_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEU9942553.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            41765..42514
                     /locus_tag="VW561_000214"
     CDS             41765..42514
                     /locus_tag="VW561_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEU9942554.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVNPYSNLNESEHYLLARHEITTNLEEICRSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            42504..42803
                     /locus_tag="VW561_000215"
     CDS             42504..42803
                     /locus_tag="VW561_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU9942555.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            42874..43809
                     /locus_tag="VW561_000216"
     CDS             42874..43809
                     /locus_tag="VW561_000216"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEU9942556.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            43895..44407
                     /locus_tag="VW561_000217"
     CDS             43895..44407
                     /locus_tag="VW561_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU9942557.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACKHLGAPYNASFYPDAPGFYCSQ
                     YIAEILPIFETIPMKFGDGEQAISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            44432..45040
                     /locus_tag="VW561_000218"
     CDS             44432..45040
                     /locus_tag="VW561_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEU9942558.1"
                     /translation="MFGELIRYLDQYEDVILREIKAQFPDVAVDKLMEEYIKAGLILR
                     ENKRYYLNFPTLESLDSLELDQEIFVREASPVYQALLEQSFETELRNQINAAILVEKT
                     DFARIKMTLSNYFYKVKQQYPLTEKQQELYDILGDVNPEYALKYMTAFLLKFLKKDQL
                     MQKCRDIFVDSLVVLGYIVQNEDGKYELAIDFDKERLTFYLA"
     gene            45141..45338
                     /locus_tag="VW561_000219"
     CDS             45141..45338
                     /locus_tag="VW561_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU9942559.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTKE"
     gene            complement(45381..46826)
                     /locus_tag="VW561_000220"
     CDS             complement(45381..46826)
                     /locus_tag="VW561_000220"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEU9942560.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVVFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQSYLVRRVYGLTFDVGVFLNISPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            46932..48554
                     /locus_tag="VW561_000221"
     CDS             46932..48554
                     /locus_tag="VW561_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU9942561.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            48757..50715
                     /locus_tag="VW561_000222"
     CDS             48757..50715
                     /locus_tag="VW561_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9942562.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKAVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            50887..>51153
                     /locus_tag="VW561_000223"
     CDS             50887..>51153
                     /locus_tag="VW561_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9942563.1"
                     /translation="MKLKLLRVDTKVIMGSFLLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKKIAKIRKKVIEGL"
BASE COUNT        15135 a         9485 c        11663 g        14870 t
ORIGIN      
        1 cacaaaaata ggcaacttgt caatagaaaa ggaacaattt aattaaaaat attctggttt
       61 ttaattaaat tgtaagtcaa tgcaagtcaa taaggaattt tcagataatt attgaaaaag
      121 agacgttttt atgttataat gatagcgatt aaattcgagg tgaaaaatgg cacatttatt
      181 agaaaaaact agaaaaatta catcaatttt gaaacgctca gaggagcagt tgcaggatga
      241 gctcccttac aacgctatta cgcgtcagtt agcggatatt attcattgca acgcctgcat
      301 tatcaatagt aagggacgtc tgcttggcta ttttatgcgt tataaaacaa atacagatcg
      361 cgtagagcaa ttcttccaaa ctaagatttt cccagatgac tacgttcaag gggctaatat
      421 gatttacgaa acagaagcaa acttacctgt tgagcatgat atgagtattt tccctgttga
      481 gagtagagat gactttccag atggcttgac gactattgca ccgattcatg tatcggggat
      541 tcgccttggt tctttgatta tttggcgtaa tgataaaaaa ttcgaagatg aggacttggt
      601 tcttgttgag attgccagta ccgttgttgg gattcagctt cttaacttcc aacgtgaaga
      661 agatgagaaa aatattcgtc gtcgtactgc tgtcaccatg gcggtcaata ccctttctta
      721 ctccgaactc cgtgctgttt cagcaatttt aggggaatta aatggaaatg aagggaagtt
      781 gactgcgtca gtgattgcag atcgtatcgg aatcactcgc tctgtgattg tcaatgctct
      841 tcgtaaactt gagtctgcgg ggattattga aagtcgctca cttggaatga agggaaccta
      901 tcttaaggtc ttgatttcag atatttttga agaagtgaag aaaagagatt actaatgaca
      961 aaggctttaa tttcgattga ttatacagaa gattttgttg ctgatagtgg gaaactaaca
     1021 gcaggtgctc cagctcaggc gatttcggat gccatcagca aggtaactcg attagctttt
     1081 gaacgaggag attatatctt ctttactatt gatgctcatg aagaaaacga ttgtttccat
     1141 ccggaaagta agttatttcc tcctcataat ctgattggga cgagtggacg gaatttatat
     1201 ggagatttgg ggatctttta tcaagagcat ggttcagaca gtcgtgtctt ttggatggat
     1261 aaacgccatt actcagcttt ttcagggact gacctagata ttcgtttgag agagcgtaga
     1321 gtgtctaccg ttatcttaac aggtgtcttg acggatatct gtgtcctaca tacagctata
     1381 gatgcctata atctaggata tgacatcgag attgttaaac cagctgttgc ttccatctgg
     1441 cctgaaaatc atcaatttgc cctaggtcat ttcaaaaata cactcggagc taagttagta
     1501 gatgaaaatc taaatgaact ttctgagtaa cctggttggt gaaaatgaaa aaatttgaaa
     1561 aaaagtgttg acaagcatcc caaaagttga tatactagta aagtaatcga tgcggggatg
     1621 gcggaattgg cagacgcgca ggactaagga tcctgtgacc gctttaggtc gtgagggttc
     1681 aagtccctct ctccgcatag gaaaagagtt agctaaagct agctcttttg tatttctcta
     1741 aaaaattaga atccagtcaa atgtagtgga gaatcagcga aaatatggga aaactgatcc
     1801 ttgtttctgc ttttgtcata gaagaataat aaagattatc ttcttcaaat tctccgatat
     1861 tctctaaagt tttgtgcaag ttgcacagaa cttttttatt tttttggtca tcttgccata
     1921 gaaatataaa gcgttttcat ctataatata attatcaaaa gacaaaagga gttcacctca
     1981 tggtagaatt gaatcttaaa aatatttaca aaaaatatcc aaacagcgaa cactattcag
     2041 ttgaagattt caacttgaac atcaaagata aagaatttat cgttttcgta ggaccttcag
     2101 gatgtggtaa atcaactaca ctccgtatga ttgctggtct tgaagacatt acagaaggta
     2161 ctgcatctat cgatggcgtg gttgtcaacg acgtagctcc aaaagaccgt gatatcgcca
     2221 tggtattcca aaactacgct ctttacccac acatgactgt ttatgacaac atggctttcg
     2281 gtttgaaatt gcgtaaatac agcaaagaag acattaacaa acgtgttcaa gaagcagctg
     2341 aaatacttgg attgaaagaa ttcttggaac gtaaaccagc tgacctttca ggtggtcaac
     2401 gtcaacgtgt tgccatgggg cgtgcgattg tccgtgatgc gaaagtattc ttgatggacg
     2461 aacctttgtc aaacttggat gccaaacttc gtgtatcaat gcgtgctgaa atcgctaaaa
     2521 ttcaccgtcg tatcggagct acaactatct atgtaactca cgaccaaaca gaagcgatga
     2581 cacttgcaga ccgtatcgtt attatgtcag ctactaagaa ccctgctggt acaggtacta
     2641 tcggacgtgt agaacaaatc ggtactcctc aagaagttta caaaaatcca gttaacaaat
     2701 ttgttgcagg attcatcgga agcccagcta tgaatttcat caacgtgaaa ttggttggta
     2761 gcgaaattgt ttctgacggt ttccgtttga aagtgccaga aggagcattg aaagttcttc
     2821 gtgaaaaagg ctacgaagga aaagaattga tctttggtat ccgtccagaa gacgtgaatg
     2881 cagaacctgc tttccttgaa acattcccag actgtgttgt aaaagcgact atctctgtat
     2941 cagaactgct tggttcagaa tctcaccttt actgccaagt tggtaaagac gagtttgttg
     3001 caaaagttga tgctcgtgac tacttgcaaa caggtgcaac agttgagctt ggatttgact
     3061 tgaacaaagc acacttcttc gatgtagaaa ctgaaaaaac aatctactaa aataaataaa
     3121 attcaaagca ctacaagaaa agatatctct ttatcaattg tagtggagag atatcagtta
     3181 atctagggag agaaacaaaa tgcttctctc ctttttgcta gggaagtcat attatgcatc
     3241 tatattgtga tgctctttaa tactcttcga aaatctcttc aaaccacgtc aacgtcgcct
     3301 tgccgtacgt atgattactg atttcgtcag ttttatctgc aacctcaaag atgtactttg
     3361 agcagcttac ggctagtttc ctagtttgct ctttgatttc cattgagtat tatttgtggg
     3421 taccatctac aagtgaagct atatgcgtaa actacgtgag caattgaatt cgaactagag
     3481 aggtaataat aaatttatgc tatagttatg gtgacttgta tgcttttgat tctagtttat
     3541 caaataatag attagaattg tcagataata tcattttgtg ttataatgaa gaaaaaacag
     3601 aggtgttcaa atgtcagaag caggtcataa gtttttagca aaattgggga aaaaacgctt
     3661 acgtccaggt ggaaagcgtg ccacagattg gttaattgca gaaggaggat tttcaaaaga
     3721 aaagagaata ctagaggttg cgtgtaatag gggaactaca gcaattgagt tggcacagcg
     3781 ttttggttgc aagataactg ctgttgatat ggatgctcaa gctttagaag tggctaaaaa
     3841 atctgctgga acggcaggtg ttgctcattt aatcagtttt gaaagagcaa atgcaatgaa
     3901 acttccttat caagatgcta gttttgatat tgttataaat gaagctatgc tgactatgca
     3961 agccgatcaa gctaagaaaa aatgtgtaat ggaatatcta agggtattaa aacctggagg
     4021 tcttctcttg acacatgatg tgcttcttaa ggaagctaaa gagtctatca gacaggaatt
     4081 atcacaagca attcatgtaa atgtaggtcc tttaactcaa gatggttggg aacaggtgat
     4141 gatagaatca ggttattgtg atgtgaaagc attgactggt gaaatgacat taatgaaatt
     4201 atcgggtatg atttatgacg aaggtttgct aggaactttg aaaatttgtg taaatgcttg
     4261 taaaaaggag aatagaaagc agtttttaac tatgtataaa atgtttgcta agaataaaca
     4321 gaaattgggc tttattgcga tggctagtta taaatcgtca aaacgttaga taattattga
     4381 agttaacttt tccttttttc tttcttaaaa aatatgctat aatagagagt aaaaaacttt
     4441 gaaagaaaga aaaagatgaa tttaaaagat tacattgcaa caattgaaaa ttatccaaag
     4501 gaaggcatta ccttccgtga tattagtcct ttgatggctg atggaaatgc ttatagctac
     4561 gctgttcgtg aaatcgttca gtatgctact gacaagaaag tcgacatgat cgtgggacct
     4621 gaagctcgtg gatttatcgt gggttgtcca gttgcctttg agttgggaat tggttttgcg
     4681 cctgttcgta agccaggtaa attgccacgc gaagttattt ctgctgacta tgaaaaagag
     4741 tacggtgtcg atactttgac tatgcacgcg gatgccatta agccaggtca acgtgttctt
     4801 attgtagatg accttttggc gacaggtgga actgttaagg caactatcga gatgattgaa
     4861 aaacttggtg gtgttatggc aggttgtgcc ttccttgttg aattggatga attgaacggc
     4921 cgtgaaaaaa tcggtgacta cgactacaaa gttcttatgc attattaatg aaaacagtcc
     4981 ctagggctgt tttctctaca ctaggatata aaaatagact ataactagtt agagaaaaac
     5041 tataattgaa aactatatct tcttacagta taataaaagg actaagtgtt tgagatttgt
     5101 cttcaaacat atgcaattat tcctgaaaga gtacagttag gagagggtta tgccgattcg
     5161 aattgataaa aaattgccag ctgttgaaat tttacggaca gagaatatct ttgtcatgga
     5221 tgatcaacgt gctgcccacc aagatatccg tcctttgaag attttaattt taaatctcat
     5281 gccacagaaa atggtcacag agacccagtt gttgcgccac ttggctaata cacccctaca
     5341 actggatatt gattttctct atatggagag ccaccgttct aaaacaactc gttcagagca
     5401 catggagacc ttctataaaa cttttcctga agtcaaggat gagtattttg atgggatgat
     5461 catcacgggt gctccagttg agcatttacc atttgaggaa gtggactatt gggaggaatt
     5521 tagacagatg cttgagtggt ctaagactca tgtctattcg acccttcata tctgttgggg
     5581 ggctcaggct gggctttatc tgcgctatgg tgtagaaaaa taccagatgg acagtaagct
     5641 atcaggtatt tatcctcagg acaccctaaa agagggtcac cttctattta gaggctttga
     5701 tgatagctat gtatcccctc attcacggca cacggagatt tctaaggaag aggtcttaaa
     5761 caagaccaat ctcgagattt tatcagaagg acctcaggtt ggggtttcta ttttggccag
     5821 tcgtgattta cgagaaattt atagttttgg tcatttggag tatgaccgtg ataccttggc
     5881 aaaagagtat tttcgagatc gtgatgcagg tttcgaccca catattccag aaaattactt
     5941 taaagatgat gatgttaatc aggtaccttg tctttgttgg tcttcatctg cagccctctt
     6001 tttcagtaat tggataaacc atgcggtcta tcaggagacg ccttttgatt ggagaaagat
     6061 agaagatgat gcatctgcat atgggtattt ataagaggaa ttatgacata tttagacgct
     6121 tttaaatcag gtatcttggt tttaccgagt gccctgctct tgcattttaa ggaactcttt
     6181 ccttctagcg acgattttct ggtttggcaa tttttctatt tgcaaaatac gacaggctta
     6241 gaagaaatgt cgccaagcca gattgctgaa aggattggca aggaaatttc ggatgtcaac
     6301 cagtccattt ctaatctgac ggaaagggga ctgctccagt atcgtactat cgaattaaat
     6361 ggcgaaattg aattgctctt tgatgctagt ttggccttgg aacgtttgga tgacctgttt
     6421 ggagcagttc attcaagttc agaccagctg acacctcaaa accagctcaa ggatttggtg
     6481 gaaaccttcc agcaggagtt gggacgattg ttgacgcctt ttgagattga ggatttgacc
     6541 aagacactaa aggaagatgg aaccagtgct gacttgatta aggaggctct tcgtgaagct
     6601 gttttgaatg gaaaaccaaa ctggaagtac attcaggcga ttttgagaaa ctggcgccat
     6661 gaaggaatca agagtgtggc tcaaattgag gccaagagag cagaaagaga agcaagcaat
     6721 cctcagttga cacaggtatc tgcagatttc agaaatgcca tggatctctg gaaggattaa
     6781 tccatgcaag caggcttgaa atccgagtaa gatttgcaag ctgtgtataa ttgtgataga
     6841 ataaaataga aaataaattg aaaaaagagg tatgtgaaat gtcacgtaaa ccatttatcg
     6901 ctggtaactg gaaaatgaac aaaaatccag aagaagctaa agcattcgtt gaagcagttg
     6961 catcaaaact tccttcatca gatcttgttg aagcaggtat cgctgctcca gctcttgatt
     7021 tgacaactgt tcttgctgtt gcaaaaggct caaaccttaa agttgctgct caaaactgct
     7081 actttgaaaa tgcaggtgct ttcactggtg aaactagccc acaagttttg aaagaaatcg
     7141 gtactgacta cgttgttatc ggtcactcag aacgccgtga ctacttccat gaaactgatg
     7201 aagatatcaa caaaaaagca aaagcaatct ttgcgaacgg tatgcttcca atcatctgtt
     7261 gtggtgaatc acttgaaact tacgaagctg gtaaagctgc tgaattcgta ggtgctcaag
     7321 tatctgctgc attggctgga ttgactgctg aacaagttgc tgcctcagtt atcgcttatg
     7381 agccaatctg ggctatcggt actggtaaat cagcttcaca agacgatgca caaaaaatgt
     7441 gtaaagttgt tcgtgacgtt gtagctgctg actttggtca agaagtcgca gacaaagttc
     7501 gtgttcaata cggtggttct gttaaacctg aaaatgttgc ttcatacatg gcttgcccag
     7561 acgttgacgg tgcccttgta ggtggtgcgt cacttgaagc tgaaagcttc ttggctttgc
     7621 ttgactttgt aaaataatca gtaagtagca aaagctaggt ggaacagcat tcagatgtct
     7681 gttacatttt ttataggaga ggaaagattg aaaataaaaa ttggattagc aagtatctgt
     7741 ttactaggct tggcaactag tcatgtcgct gcaaatgaaa ctgaagtagc aaaaacttcg
     7801 caggatacaa cgacagcttc aagtagttca gagcaaaatc agtcttctaa taaaacgcaa
     7861 acgagcgcag aagtacagac taatgctgct gcccactggg atggggatta ttatgtaaag
     7921 gatgatggtt ctaaagctca aagtgaatgg atttttgaca actactataa ggcttggttt
     7981 tatattaatt cagatggtcg ttattcgcag aatgaatggc atggaaatta ctacctgaaa
     8041 tcaggtggat atatggccca aaacgagtgg atctatgaca gtaattacaa gagttggttt
     8101 tatctcaagt cagatggggc ttatgctcat caagaatggc aattgattgg aaataagtgg
     8161 tactacttca agaagtgggg ttacatggct aaaagccaat ggcaaggaag ttatttcttg
     8221 aatggtcaag gagctatgat acaaaatgaa tggctctatg atccagctta ttctgcttat
     8281 ttttatctaa aatccgatgg aacttatgct aaccaagagt ggcaaaaagt gggcggcaaa
     8341 tggtactatt tcaagaagtg gggctatatg gctcggaatg agtggcaagg caactactat
     8401 ttgactggaa gtggtgccat ggcgactgac gaagtgatta tggatggtgc tcgctatatc
     8461 tttgcggcct ctggtgagct caaagaaaag aaagatttga atgtcggctg ggttcacaga
     8521 gatggtaaac gctatttctt taataataga gaagaacaag tgggaaccga acatgctaag
     8581 aaaatcattg atattagtga gcacaatggt cgtatcaatg attggaaaaa ggttattgat
     8641 gagaacgagg tggatggtgt cattgttcgt ctaggttata gcggtaaaga agacaaggaa
     8701 ttggcgcata acattaagga gttaaaccgt ctgggaattc cttatggtgt ctatctctat
     8761 acctatgctg aaaatgagac cgatgctgag aatgacgcta aacagaccat tgaacttata
     8821 aagaaataca atatgaacct gtcttaccct atctattatg atgttgagaa ctgggaatat
     8881 gtaaataaga gcaagagagc tccaagtgat acagacactt gggttaaaat catcaacaag
     8941 tacatggaca cgatgaagca ggcgggttat caaaatgtgt atgtctatag ctatcgtagt
     9001 ttattacaga cgcgtttaaa acacccagat attttaaaac atgtaaactg ggtagcggcc
     9061 tatacgaatg ctttagaatg ggaaaaccct tattattcag gagaaaaagg ttggcaatat
     9121 acctcttctg aatacatgaa aggaatccaa gggcgcgtag atgtcagcgt ttggtattaa
     9181 gcgatgattt gaaagaggga tgtgatagta gcaccctctt tttctttgtt ttatgatagt
     9241 tcatcctcga gtaaattcaa gatcttgctc ggaaatgaag cttatatagt agattgaata
     9301 tagataaaga ccttgtgatt ggtaaaacat tttagaaatt catttacctt tcctaatcga
     9361 cttggtttca tcttatttca atctattata gtattgggga atttcttcaa accacatcag
     9421 cttggtcagt cctacctgcg acctcaaaac ttgtgctttg gtcaagctgg gtttagtttc
     9481 ctagtttgct gatggatttc cattgactat aagcatccaa ccctcttttt tgtcttctaa
     9541 agaattctta aattatcagt ctattgcaac ttttctcata taagttcttt gtcttgctat
     9601 tggttttcct tagtagtata ctaaggtagt aatcattaag aagtggttac aaaaaataat
     9661 gaatgaggta aagaaaatgg tagaattgaa aaaagaagca gtaaaagacg taacatcatt
     9721 gacaaaagca gcgccagtag cattggcaaa aacaaaggaa gtcttgaacc aagctgttgc
     9781 tgatttgtat gtagctcacg ttgctttgca ccaagtgcac tggtatatgc atggtcgtgg
     9841 tttccttgta tggcatccaa aaatggatga gtacatggaa gctcttgacg gtcaattgga
     9901 tgaaatcagc gaacgcttga ttacactcgg tggaagccca ttctctacat tgacagagtt
     9961 ccttcaaaat agtgaaatcg aagaagaagc tggtgaatac cgtaatgttg aagaaagctt
    10021 ggaacgtgtt cttgttatct accgttactt gtcagaactt ttccaaaaag gtttggatgt
    10081 cactgatgaa gaaggtgacg atgtgacaaa cggtatcttt gcaggcgcta aaactgaaac
    10141 agataaaaca atttggatgc ttgcagccga acttggacaa gcacctggtt tgtaagaaat
    10201 aaggaaacat agataaatct gtaggaaatt tcttacagat tttttctatt ttgtaagcta
    10261 ttccaaacca gtcttttttt gagttttttg ataaaatagt actatcagtg aaaaggatgg
    10321 aagcatgact aagaaaatcg tagctatttg ggcccaggat gaagagggtg tgattggtaa
    10381 ggacaatcgt ctgccttggc atttgccagc agaattgcag cactttaaag aaacaactct
    10441 gaatcatgct atcttgatgg ggcgtgtgac ctttgatggg atggggcgtc gcttgcttcc
    10501 aaaacgggaa accctgattt tgacgcgtaa tccggaagaa aagatagatg gggttgctac
    10561 ttttcaggac gtccagtctg ttcttgactg gtatcaggct caagaaaaga atctctacat
    10621 tatcggtggg aagcaaattt ttcaggcttt tgaaccttac cttgatgaag tgattgtcac
    10681 tcacattcat gctcgggtgg aaggagatac ctatttccct gaagaacttg acttgtctct
    10741 ttttgagaca gtttcaagca aattttacgc caaagatgag aagaatcctt atgattttac
    10801 catccaatac cgcaagagaa aggaagtcta atggaacgga gtttatttgg tctattcacg
    10861 gcttttctgt gtttcatctg ctttctggca ggagcacaag cttttcgtaa aaagcgttac
    10921 gggctttcta tcctcctttg gttaaatgcc tttaccaatc tggtcaatag tattcatgct
    10981 ttttacatga ccttatttta gatagaatga cagtatagaa tggaaggaaa tcatgtctac
    11041 aaatagaaaa aatgatatga tggtttattg ctcattttgt ggcaaaaacc aagaagaagt
    11101 acaaaaaata attgctggca acaatgcttt tatttgtaat gaatgcgtgg agttagctca
    11161 ggaaatcatt cgagaagaat tggttgagga agtcttggca gacttgtctg aggtgccaaa
    11221 accaattgaa ctcctccata tcttgaacca ctatgtaatt ggccaagatc gtgccaagcg
    11281 tgccttggca gtggcggttt ataaccacta caaacgcatc aatttccacg atacacgcga
    11341 agagtcagaa gatgtggatt tgcagaagtc aaacattttg atgattggcc caactggttc
    11401 agggaaaact ttccttgccc agaccttggc taagagcttg aatgtacctt ttgctattgc
    11461 ggatgcgaca gctctgacgg aggctggtta tgtgggtgag gacgtggaaa atatcctcct
    11521 caaactcttg caggttgctg actttaacat cgaacgtgca gagcgtggca ttatctatgt
    11581 ggatgaaatt gacaagattg ccaagaagag tgagaatgtg tctatcacac gtgatgtttc
    11641 tggtgaaggg gtgcaacaag cccttctcaa gattatcgag ggaactgttg ctagcgtgcc
    11701 gcctcaaggt ggacgcaaac atccacaaca agagatgatt caagtggata caaaaaatat
    11761 cctcttcatc gtgggtggtg cttttgatgg tattgaagaa attgtcaaac aacgtctggg
    11821 tgaaaaagtc atcggatttg gtcaaaataa taaggcgatt gacgaaaaca gctcatatat
    11881 gcaagaaatc atcgctgaag acattcaaaa atttggtatt atccctgagt tgattggacg
    11941 cttgcctgtt tttgcggctc ttgagcaatt gaccgttgat gacttggttc gcatcttgaa
    12001 agagccaaga aatgccttgg tgaaacaata ccaaactttg ctttcttatg atgatgttga
    12061 gttggaattt gacgacgaag cccttcaaga gattgctaat aaagcaatcg aacggaagac
    12121 aggggcgcgt ggacttcgct ccattatcga agaaaccatg ctagatgtta tgtttgaggt
    12181 gccgagtcag gaaaatgtga aattggttcg catcactaaa gaaactgtcg atggaacgga
    12241 taaaccgatc ctagaaacag cctagaggtg actatggaac ttaatacaca caatgctgaa
    12301 atcttgctca gtgcagctaa taagtcccac tatccgcagg atgaactgcc agagattgcc
    12361 ctagcagggc gttcaaatgt tggtaaatcc agctttatca acactatgtt gaaccgtaag
    12421 aatctcgccc gtacatcagg aaaacctggt aaaacccagc tcctgaactt ttttaacatt
    12481 gatgacaaga tgcgctttgt ggatgtgcct ggttatggct atgctcgtgt ttctaaaaag
    12541 gaacgtgaaa agtgggggtg catgattgag gagtacttaa cgactcggga aaatctccgt
    12601 gcggttgtca gtctagttga ccttcgtcat gacccgtcag cagatgatgt gcagatgtac
    12661 gaatttctca agtattatga gattccagtc atcattgtgg cgaccaaggc ggacaagatt
    12721 cctcgtggta aatggaacaa gcatgaatca gcaatcaaaa agaaattaaa ctttgacccg
    12781 agtgacgatt tcatcctctt ttcatctgtc agcaaggcag ggatggatga ggcttgggat
    12841 gcaatcttag aaaaattgtg aggaaaagaa aatggcaaaa acaattcata cagataaggc
    12901 cccaaaggct atcggaccct atgttcaagg aaaaatcgtt ggcaaccttt tgtttgctag
    12961 cggtcaagtt cccctatccc ctgaaactgg ggaaattgta ggagagaata tccaagaaca
    13021 gacagagcaa gtcttgaaaa acatcggtgc tattttggca gaagcaggaa cagactttga
    13081 ccatgttgtc aaaacaactt gtttcttgag cgatatgaac gactttgttc cttttaatga
    13141 ggtttaccaa acggccttca aagaggaatt cccagctcgt tcagctgtgg aggtagctcg
    13201 tcttcctcgt gatgtaaaag tcgaaattga agtcatcgca gagattggat aagctagttg
    13261 aagtttggtg ttgccaaact tcttttgata taaggagaaa aagatgacaa agaaacaact
    13321 tcacttggtg attgtgacag ggatgagtgg cgcagggaaa actgtagcca ttcagtcctt
    13381 cgaggatcta ggttatttca ccattgataa tatgccgcca gctctcttgc ctaagttttt
    13441 gcagctggtt gaaattaagg aagacaatcc taagttggcc ttggtagtgg atatgcgtag
    13501 ccgttctttc ttttcagaga ttcaagctgt tttggatgag ttggaaaatc aagatggttt
    13561 ggatttcaaa atcctctttt tggatgcggc tgataaggaa ttggtcgctc gttacaagga
    13621 aaccagacgg agtcacccac tagcagcaga cggtcgtatt ttagatggaa tcaagttgga
    13681 acgtgaactc ttggcacctt tgaaaaatat gagccaaaat gtggtggata cgactgaact
    13741 cactccacgt gagctgcgca aaacccttgc agagcagttt tcagaccaag aacaagccca
    13801 gtctttccgt atcgaagtca tgtctttcgg atttaagtat ggaatcccga ttgatgcgga
    13861 cttggtcttt gatgtccgtt tcttgccaaa tccctattat ttaccagaac tgagaaacca
    13921 aacgggtgtg gatgaacctg tttatgatta tgtcatgaac catcctgagt cagaagactt
    13981 ttatcaacat ttattggcct tgattgagcc gattctgcca agttaccaaa aggaaggtaa
    14041 gtccgttttg accattgcca tgggatgtac gggtggacaa caccgtagtg tggcatttgc
    14101 taaacgcttg gtgcaggact tatccaagaa ttggtctgtt aatgaagggc atcgcgacaa
    14161 agaccgcaga aaggaaacgg taaaccgttc atgagaaaac caaagataac ggtgattggt
    14221 ggagggactg gaattcccgt cattctaaaa agtctgcggg aaaaagatgt ggaaatcgca
    14281 gctatcgtga cggtggcaga tgatggtggt tcttcaggtg aactccgaaa aaatatgcaa
    14341 cagttgacac cgccaggtga ttttcgtaat gtccttgtgg ccatgtcgga tatgcctaag
    14401 ttttatgaga aggtctttca gtatcggttc tctgaggatg ccggagcctt tgctggccat
    14461 ccattgggaa atctcatcat tgctggcttg tcagaaatgc agggttcaac ctataatgcc
    14521 atgcagttat tgagcaaatt tttccataca acagggaaaa tttatccttc cagtgaccat
    14581 cctttgaccc ttcatgcagt ctttcaggat gggacagaag tggctggaga gagtcatatt
    14641 gtagaccatc gaggcataat tgacaatgtc tatgtgacca atgccctaaa cgatgatacg
    14701 cctctggcca gccgtcgagt agtgcagacc atccttgaaa gtgacatgat tgtcctaggg
    14761 ccaggttctc tctttacctc tattttgccc aatatcgtga ttaaggaaat tgggcgggct
    14821 cttttggaaa ccaaggcaga aattgcctat gtctgcaata tcatgaccca acgtggggag
    14881 acggaacact ttacagatag cgaccacgtg gaagtcttgc atcgtcacct tggtcgccct
    14941 tttatcgaca ctgtcttggt gaatattgaa aaagtgcctc aggaatacat gaattccaac
    15001 cgttttgatg aatacttggt gcaagtggaa cacgattttg taggtctttg taagcaagtt
    15061 tcgcgcgtga tttcatctaa cttccttcgt ctggaaaatg gcggtgcctt ccacgatgga
    15121 gatttgattg tggacgagtt gatgcgcatt atacaggtga aaaaatgagt ttcacagtag
    15181 cagtaaaaga agaaatcctg ggccaacacc atctgagctg gcatgaatta tctgccatta
    15241 tcaaaatgtc tggtagcatc ggtctctcga cttcgggctt gactttgtca gttgtgacag
    15301 aaaatgccaa actggcccgt cacctctatg agtcctttct tcatttctat gaaatcaaat
    15361 ctgaaattcg tcaccaccaa cgtagcaatc ttcgcaagaa tcgcgtctat accgttttta
    15421 cagatgaaaa ggtgcaggat ttgttaagtg atttgcactt ggcagactct ttctttggcc
    15481 tggaaacagg tattgatgag gcgattttat cggatgagga agcaggtcgt gcctatctct
    15541 gtggcgcttt cttggcaaat gggagcattc gtgaccctga gtcaggcaag taccagttgg
    15601 aaatcagttc tgtttatctg gaccacgcgc aaggaattgc ctcccttctc caacagtttt
    15661 tactggatgc caaggtgctt gagcgcaaga agggggctgt gacctatctc cagagagcag
    15721 aagacattat ggacttcttg atagtaatcg gagccatgca ggcacgtgat gattttgagc
    15781 gggttaagat tttgcgagaa acccgtaacg acctcaatcg ggctaataat gccgagacag
    15841 ctaatatcgc tcggacagtt tctgccagca tgaagaccat taacaatatc agtaaaatca
    15901 aagatatcat gggcttagaa aatttgccag tagatttgca ggaagtggcg caactgagga
    15961 ttcagcaccc agactactct atccagcagt tagcagatag tctcagcacc cctctgacca
    16021 aaagtggtgt caatcacaga ctcagaaaaa tcaataagat agcggatgaa ttataaaacc
    16081 acgaatccta tgtgactcgt ggttcttttt tataaactag tagagtgttt tggttgtact
    16141 ttttgttcag ggtcaatgta gttgatagcg ttgttgacag cagttggagc ttctccgagg
    16201 cctgtcgcaa tcagatcaat ttttccgtca tagtagcagc agtcaccgat agcatagata
    16261 cctgcttggc tggattcctg tttgctgttg acgataatct tgtgacggtt gaggtcgagc
    16321 ccccagtttt taaggttacc gacagaagat ttgaaaccat agttgacaaa gaggtggtct
    16381 aggtcaatgg tttcagtttc atcagatttg acttttgtga tttcaagttt atcaagtgtt
    16441 tttccatttc caaggagttg gccaggggcg aatggtgtct tgatggttac agatgattct
    16501 tgcaaggctt gaacactgtg ttccaaggca cggaaattat ctctgcggtg aacaagggta
    16561 gttggtgcga ttttttcaaa agccaaagcc caatccacag ccgagtctcc cccaccaaga
    16621 atcgtcactt tcttaccagc gtattgctga atgttagaaa cgtggtagtg gatattttca
    16681 tagccctcaa ccccttcaag ttccagcgga cgtggtttga aggcaccgcc acccatagcg
    16741 atgataactg ttttagtcag gtgacttcct ttagaagttg tgatggcaaa ttcttcttct
    16801 tgtttgtcaa tctcaagaac cgtttcattg agatgaatag gggtatcaaa tccatttagc
    16861 tgttcaatca agcggttagt caactcttct ccagtcaggt ttgggaagcc tggtacgtct
    16921 aggatttcct tttcagggta gagaatagca ggttgtccac ctagctgggg aagagagtcg
    16981 atgatttgaa ccttggcttg gcgtaggtgg gcataaaagg ctgcaaaaag cccgacagga
    17041 ccaccaccca caatggtaat atcatagagt tgagacatag tttctccttt gttttttcta
    17101 gtcagtttat tttatcatat ttttgttcaa tgtaaaatgg actaggatag ggaaaaaaat
    17161 gcccgaaaat ggtataatag gacgaaagtg ttttgatagg gaggatatca gggatgaatt
    17221 ttcaacaatt atccaatctg caatattgga ccagtttgtt tgcaagtcca tgggcgatag
    17281 ctatcaatct gattgatatt ttgattgttg cttatatttt ataccatttt acaaaagcta
    17341 ttgcaggaac caagattatg attttggtac gtggagtttt ggtatttatt ttagctcaaa
    17401 tccttgcaaa tatgattggt ttgactacga tttcttggtt aatcaatcaa attattactt
    17461 atggggttat tgcggcggtt gttatcttct ctccagagat tcggactggt ttggaacgtt
    17521 tgggaagagc gacagatttc ttttccaatg cccctattag tgctgaggaa cagatgattc
    17581 gtgcctttgt taagtctgtt gaatacatga gtcctcgtaa aatcggggcc ttggttgcta
    17641 ttcaacgtgt acgtaccttg caggagtata tttcgacagg aatccccttg gatgctaaga
    17701 tttctgcaga acttctcatt aacattttta ttcccaacac tcccctacat gacggtgcgg
    17761 tgattatcaa agaagaacgt atcgctgtga cgtctgccta tctgcccttg acaaaaaaca
    17821 caggtatttc caaggaattt gggaccagac accgggcggc tatcggttta tcagaagtct
    17881 cagatgcctt gacttttgtc gtatcagagg aaacgggagg aatttcgata acctataatg
    17941 gaaggtttaa gcacaaccta acacttgatg aatttgaaac agaattacgt gaaatcttac
    18001 ttccaaaaga ggaagtgggt cttagtttta aagaacgatg gctaggagga tggaaacatg
    18061 aaaaaaaata gtttatatat catatcctca ctcttttttg cttgtgtctt atttgtctat
    18121 gctacggcga cgaattttca aaacagtatc agtgctaggc aggtaaaaac ggaaacctat
    18181 actaatacag taacaaatgt ccctattgac atacgctata atagtgataa gtattttatt
    18241 agcggttttg cttcagaagt atcagtggtc ttgactggtg caaatcgcct atcgctagct
    18301 agtgaaatgc aagaaagtac acgtaaattc aaggttactg ctgacctaac agatgccggt
    18361 gttggaacga ttgaagttcc tttgagcatt gaagatttac ccaatgggct gaccgctgtg
    18421 gcgactccgc aaaaaattac agtcaagatt ggtaagaagg ctcagaagga taaggtaaag
    18481 attgtaccag agattgaccc tagtcaaatt gatagtcggg tacaaattga aaatgtcatg
    18541 gtgtcagata aagaagtgtc gattacgagt gaccaagaga cattggatag aattgataag
    18601 attatcgctg tcttgccaac tagcgaacgt ataacaggta attacagtgg ttcagtacct
    18661 ttgcaggcaa tcgaccgcaa tggtgttgtc ttaccggcag ttatcactcc gtttgataca
    18721 ataatgaagg tgactacaaa accagtagca ccaagttcaa gcacatcaaa ttcaagtaca
    18781 agcagttcat cggagacatc ttcgtcaacg aaagcaacta gttcaaaaac gaattaaaaa
    18841 tagaaaagga ttttataaca atgggtaaat attttgggac tgatggagtc cgtggagaag
    18901 ctaacctaga actaacacca gaattagcct ttaaactagg acgttttgga ggctatgttc
    18961 ttagtcaaca tgaaacggaa gcgccgaaag tctttgtagg acgtgacaca cgtatttcag
    19021 gggaaatgct ggaatcggcc ttggtggcag gtctcctttc agtagggatt cacgtataca
    19081 aacttggtgt ccttgcaaca ccagcagtag cttacttggt tgaaactgaa ggagcaagtg
    19141 ccggtgtcat gatttctgct agccacaacc cagcccttga taacggaatc aagttctttg
    19201 gcggtgatgg cttcaaacta gatgatgaaa aagaagcaga aattgaagcc ttgctagatg
    19261 ctgaggaaga cactcttcct cgtccaagtg cagaaggctt aggaattttg gtagattatc
    19321 cagaaggctt gcgtaagtat gaaggatacc ttgtttcaac tggaactcct cttgatggaa
    19381 tgaaggttgc cttggataca gctaatggag cagcttctac cagtgcccgt caaatctttg
    19441 cagaccttgg tgcccaattg acggttatcg gggaaacacc agacggtctt aacatcaacc
    19501 ttaatgttgg ttcaacacat ccagaagccc ttcaagaagt ggtcaaagaa agtgggtcag
    19561 ctattggttt ggcctttgat ggagacagtg accgcttgat tgctgttgat gagaatggtg
    19621 acatcgtcga tggtgacaag attatgtaca tcatcggaaa atacctttct gaaaaaggac
    19681 aattggctca aaatacaatt gtgacaactg ttatgtctaa ccttggtttc cacaaggcct
    19741 tgaatcgcga aggtattaac aaggcagtta ctgcagttgg tgaccgctac gttgttgaag
    19801 aaatgagaaa atcaggttac aaccttggtg gtgaacagtc tggtcacgtt atcttgatgg
    19861 attacaatac cacaggtgat ggtcaattat cagcagttca attgactaaa atcatgaagg
    19921 aaactggtaa gagcttatca gagttggcgg cagaagtaac gatttatcca caaaaattag
    19981 ttaatatccg agtggaaaac gtcatgaagg aaaaggccat ggaagtgcca gctatcaagg
    20041 ccatcatcga gaagatggaa gaagaaatgg cggggaacgg ccgtatcctt gttcgtccaa
    20101 gtggaacaga acccctcttg cgtgttatgg cagaagcgcc tacaacagaa gaagtagact
    20161 actatgttga taccatcaca gatgtagttc gtgctgaaat tgggattgac taaatcctag
    20221 aaaactagat gaaaataaaa aattatggta caatagctta taatattgta gccgatggag
    20281 aaagacatga atcttaaacg tgaacaagaa tttgttagtc agtatcattt tgatgcgcgt
    20341 aattttgaat gggaaagtga aaatggagct cctgaaacca aggtggatgt gaatttccaa
    20401 ttgctccaac atgaccaaga aaatcaagtg acttccttga ttgtcatctt gagctttatg
    20461 attgtatttg ataagtttgt cattagtgga acaatctcac aagtaaacca tatcgatggc
    20521 cgtattgtca atgaaccaag tgaattgaac caagaagagg tggaaacctt ggctcgtcca
    20581 tgtttgaaca tgctcaatcg tttgacttac gaagtgactg aaattgcatt agacttacca
    20641 ggaattaatt tggagttcta atatgaaatt agctgttttc acagattcct ctgcttatct
    20701 cagtgaagag accttgcaaa gagaagactt gtttgtcttg gatattcctg tcaatattga
    20761 tggtgaggaa tatgtcgaag gcatcaatct gtctgctgag gaattttacc aaaaaatggc
    20821 tcaggcttct gaattgccta agaccagtca accaagtatt gccaagttag atgagatctt
    20881 aacttcgctc aaagaacaag gctatacaca tgccttgggg cttttcctat cttctggaat
    20941 ttcaggtttt taccaaaata tccagtatat ggtcgatgac tatgagggct taaccattgc
    21001 tttcccagac actttgatta caagtgctcc cctaggcatc atggttgaaa gcgtctttaa
    21061 ttggcgtgat cagggcgatg attttgccag cattcaggat aagctagcca ttcaaatcag
    21121 ccgtacgtca gcctttatca tggtagatga cttggatcat ttggtaaaag gtggacgcct
    21181 ttcaaatggg gctgccattt tgggcaattt gcttagcatt aagccaatcc tttattttaa
    21241 cgatcaaggt gtgattgaag tttacgaaaa agttcgtact gaaaagaagg ccaccaagcg
    21301 cttaattgaa attatcaagg aaacaacggc ttcaggtcaa taccgggtca ttgtcattca
    21361 tgggaatgct cctgaaaagg ctgaagaatt gcgtcagcac ttgcttgatt ttggcttggg
    21421 ttcggatgtt tcacttgcta catttggtag tgtcattgga acgcacctag gagcaggaag
    21481 tattgctctg ggttatattc cagtgattta gttggcgctt ctaggctagt taagatgcct
    21541 tgtatcttag aaattgaaca cggactacct gcctcttgaa aaaagatgcc tgtcttgcct
    21601 ttcgtggaaa gtcagtgcca ttccctattt ttcagaggca gctaaggtcc tttgtatctt
    21661 gataattgaa cacgcctgga accctgtgtg aaaaagatag ttcttccaag aagtagacac
    21721 ttcttgattg gaattcctat tttcactttg tgttcttacg gactttgtat cttagacagg
    21781 agtagagatg agtattcgag taattattgc cggttttaag ggaaagatgg gccaggctgc
    21841 ttgtcagatg gtattgactg atccagactt ggacttggta gcagttttgg atccttttga
    21901 gtctgagtca gaatggcagg gtattcctgt tttcaaggat aaggctgatt tagctggttt
    21961 tgaagcggat gtctgggtag attttactac tccagctgtt gcctacgaaa atacacgttt
    22021 tgctcttgaa aatggctttg ctccagtagt tggaacgact ggtttcacga gtgaagaaat
    22081 tgcagagtta aaagaatttt ctcgtgccca agacttgggt ggcctgattg cccctaactt
    22141 tgccttgggt gctgtcttac tcatgcaatt tgcgacgcag gctgccaaat atttcccaaa
    22201 tgtggagatt attgagctcc atcatgacaa gaaaaaggat gctccgagtg gaacagccat
    22261 taaaacagct gagttgatgg cagaggttcg agagtccatt cagcaaggcg cagcagatga
    22321 ggaagagctg attgctggtg ctcgtggtgc tgactttgat ggtatgcgca tccactcagt
    22381 tcgtttgcca ggcttggtag ctcatcaaga agtcatcttt ggcaatcagg gagaagggtt
    22441 gaccctccgt catgactcct atgatcgcat ctccttcatg acaggagtca atttgggaat
    22501 taaagaagtt gtcaagcgtc atgagcttgt ctatggatta gaacacttat tatgagatta
    22561 acgcaaatgc cttctgaatt tcagaaggct ttaccagtat tagaaaaaat taaagaagca
    22621 ggctttgagg cttattttgt tgggggctct gttcgagatg cccttctcca tagccctatc
    22681 cacgatgtgg atattgcgac gtcttcttat ccagaagaga ccaagcagat ttttccgcga
    22741 acagccgata tcggaatcga gcatggaacc gtcttggtct tagatgggga tgaggagtat
    22801 gaggtaacaa cctttcggac agaggatgtc tatgtggact atcgcagacc cagtgcggtt
    22861 tcctttgtgc gctcgctaga agaagacctc aaacgccgtg atttcacagt caacgccttt
    22921 gccttggatg agacaggaga aatcgttgac ttgttccatg gtttagaaga tttggaaaag
    22981 caagtcttgc gagcagttgg agtggctagt gagcgtttca acgaagatgc tttacggatt
    23041 atgcgtggtt tccgttttca ggctagtctt ggttttgcac ttgagccaga aacatttaaa
    23101 gctatgaaga ccttgacgcc gcttttggag aaaatttctg tagagcgtac cttcgtcgag
    23161 tttgataaac tcttgctggc tccattttgg agaaggggct tggcttccat gattgagagt
    23221 caagcttatg actatctccc tgatatggca tctagccagg acaagctcaa cagactgttt
    23281 gatttggaga ctgattttac ttttgaatcc tctgaacaag cctgggcggc tttactatgg
    23341 gctttggaga ttgaaaatgc gcagtcattt ttgaaatctt ggaagacctc acgccagttt
    23401 gccaagcaag ttcaggattt gctgattatt ttggctctgc gtgaaaatgg agaattgagc
    23461 aagcgagatt gttatcgctt tgatatagat ttgcttttac aggctgaaaa tcttcgtcaa
    23521 gctcaaggaa aagaagtcaa cctacaagcc atcacagaaa aataccaaag cttgactatc
    23581 catgacaaga aagagattca gattaatggc gatattttga tcaaggaata tggctatcag
    23641 ccaggcccag acttgggaga gattttaaca gagattgagt ttgccattgt cgatggagaa
    23701 ttggagaata atcgtgaagc catccatgct tacctgaggg agaaaaaatg agtgatttta
    23761 tcgttgaaaa actaagtaaa tccgttggtg acaagaccgt ttttagggat atttccttta
    23821 ttatccatga cttagacaga attggtttaa tcggtgtcaa tgggactggc aagaccaccc
    23881 ttttggacgt cctttctggt gtttctggat ttgatgggga tgtcagtcct ttttcagcta
    23941 aaaatgatta ccagattggt tacttgactc aggatcctga ttttgatgat agaaagacag
    24001 ttttggatac ggttctatct agtgaactca aggaaatcca gctcattcgt gagtatgaat
    24061 tgattatgct cgactatagt gaggacaagc aggcgcgttt ggaacgtgtc atggcagaga
    24121 tggactctct ccaagcttgg gaaatcgaga gtcaggtcaa gaccgttctt agtaaattgg
    24181 gcattcaaga cttatctact cctgttgggg aattgtcagg tggtctgaga agacgggtac
    24241 agttggcaca agtcttactt ggcaaccacg accttttgct tttggatgag ccgaccaacc
    24301 atctggatat tgcgattatt gagtggctga ccctcttttt gaaaaattct aagaagaccg
    24361 tcctttttat cactcacgat cgttatttct tagacgcttt gtcaacacgg attttcgagt
    24421 tggagcgagc aggcttgaca gaatatcagg gcaattacca ggactatgtt cgccttaagg
    24481 cagaacagga cgagcgcgat gcagctctac ttcataaaaa agaacaactc tacaaacaag
    24541 aactggcctg gatgcgcaga caaccgcagg cacgtgcgac caagcaacaa gctcgtatca
    24601 atcgtttcca tgatctgaaa aaggaagttt caggcagtag tgctgagaca gacttgacta
    24661 tgaactttga aaccagtcgg attgggaaga aagtcatcga gtttcaggat gtttcctttg
    24721 cctatgaaaa caagcccatt ttgcaagatt ttaatctctt ggtacaggtc aaagatcgta
    24781 tcggaatcgt tggggacaac ggtgttggaa aatcaacctt actcaactta attgcaggaa
    24841 gtcttgagtc aactaaagga caagttgtga ttggggaaac tgttcgcatc gcctatttct
    24901 ctcagcaaat tgagggcttg gatgaaagca agagagttat caattacctg caggaagtgg
    24961 cagaggaggt caagaccagt ggtggttcta cgacttcgat tgcagagttg ctggagcagt
    25021 tccttttccc acgttcgacg catggaacct tgattgagaa attgtcaggg ggtgagaaaa
    25081 aacgtcttta tctcctcaaa ctgcttttgg aaaaaccaaa tgttcttctt ttagacgagc
    25141 caaccaatga cctagatatt gcaactttga cagtcttgga gaatttcttg caaggttttg
    25201 caggtcccgt tttaacagtc agtcacgacc gctatttctt ggataaggta gcgaccaaga
    25261 ttctcgcttt tgaggatggc aagattcgtc ctttctttgg tcattacacc gactatcttg
    25321 atgaaaaagc ttttgaaaca gatatggcca atcaagtgca aaaggccgaa aaggaaaaag
    25381 tggtcaaggt tcgagaagac aagaaacgca tgacctacca agaaaagcag gagtgggcaa
    25441 gtattgaagg tgatattgaa accttggaaa aacgtatcgc tgctattgaa gaggaaatgc
    25501 aggctaacgg ctctgacttt ggtaagctgg ctactctcca aaaagaattg gatgagaaaa
    25561 atgaagcact ccttgaaaaa tacgaacgct atgagtatct cagtgaattt gatagttaag
    25621 actattcgaa aatcaaagtg caaactatgt cggcttcaca ttgccgactc aagtacagcc
    25681 tgtgaccggc ttcctaattt gcactttgat ttttattgag tataaaagaa tagaaaaatc
    25741 ccgtctcatg aaaggatttc tctattcttt tttggtttct tgatgaaaga tattttgcca
    25801 ggtttcgtgg cgacgccaga tactggtatg gatgatacca tctaactcat agcagatgag
    25861 tttggttttt tgactgatgg aagtaatttg aatgtccttg atagcagaat ttaactcttt
    25921 tttggtctta taagcctctt tatccatctg ctctccatct tggcgaatat agacaaaatc
    25981 atcagtcaag agttcttcta gttggtttcc ttggtcaatc aattgttcac gcataagcaa
    26041 ttttttatag acattgtcta aaatttcatc ctcagggata ggtgctggtt cgatatggac
    26101 atcggtatca aagacgccaa aacgctcctc cagcatagac tcgacctgat ccgcgatttc
    26161 atggctttca aaaacagaca agtcaggatt catctctagt gtaatatcca ggtagatgtt
    26221 gctaccgtag gtgcgacctc tttgcgattt gaccttgctg attttgggaa tttccatgat
    26281 agccttttgg tagtcctcga gcaggcggtc gtcaaagcca tctgaaagac taaaggaaga
    26341 ctcgatgaag atatcatagg cagtcttcaa gataaagaaa gtgatgatga tagcaaccag
    26401 tttatccaca atcggataat tgaaactact agctaggatg gcaatggcgg ttccaagtga
    26461 ggtaacagcg tcagaaagat tgtccttagc agctgccttc agcgccttgg agttggattt
    26521 cttactgagg cgagtattgt agagatagac cacaaacata atcgctgcag aaatgattcc
    26581 tagagttgca ccaagaggat caatgaccgt ttcttcccga ctgagaatct tttgaatggt
    26641 atctcttaga acatcgaaac cgacatagaa catgatgata gaagtgatca agcttgccaa
    26701 atcttcaatc ttccaatgac caaaacggtg gtcacggtct gcaggctggc gcgccatccg
    26761 aatcccgatt aagagggcca catttccaat gatgtccgat acgttattaa aaccatcggc
    26821 caccaaactg gatgaatgaa ggagatgacc agctgctaat ttggctgcag acaagatcaa
    26881 ataggtcgaa atactgataa tggctccacg ctcagctaac ttgagatttg agatagattg
    26941 cttcattcaa cttcctttct agtcacatag cattctacca ttatactggt tttcaaggaa
    27001 aaattcaaat aaggcagttt ttactctttg aaaactcccc aaaatttggt ataatggtac
    27061 tactcaaggg agtagctggc agaaacctgt gatagtgtcg tcattccgaa ttttatgctg
    27121 aaaagtatgc tttccggccc tatcttaaac agcgagactt gttatgatta acaggtctct
    27181 ttttgtttgt cgtgaaaaga tatgacagaa agagagtctg ttttgcacac aatgtcaagg
    27241 aggagacaca tgtcaaaaga acaaaaacgc caagcgtttt atactcagag ccctgaagag
    27301 gtcttgcagg ctgtggatgc gaccgagcaa ggtttgtcat caagtgaggc ggaaaagcgc
    27361 cttgccgaat ttgggcacaa tgaactcgaa gaaggcgaga aacgatcaat cttggtcaaa
    27421 ttcatcgagc aatttaagga tttgatgatt atcatcctag ttgcggcagc aatcttgtca
    27481 gtcgtgactt ctggtgggga agatatcgca gatgccatta tcatcctagc tgtggttatc
    27541 atcaacgctg cctttggtgt ttaccaagaa ggaaaagctg aagaagctat tgaagccctc
    27601 aaatccatgt ctagtccagt tgcccgcgtt cttcgtgatg gacacatggc agagattgac
    27661 tctaaagaat tggtaccagg cgatatcgtt gcccttgaag caggtgacgt ggtaccagcg
    27721 gacctacgtt tgctagaagc caactctctt aaaattgaag aagcagcctt gacgggtgaa
    27781 tctgtaccag tcgaaaaaga cttgtcagtc gagcttgcga cagatgctgg tattggtgac
    27841 cgtgtcaaca tggccttcca aaactcaaac gtaacctatg gtcgtgggat gggtgttgtt
    27901 gtcaatacag gtatgtacac tgaagttggt catattgctg gtatgcttca agatgcggat
    27961 gagactgata caccactcaa acaaaacttg aacaaccttt ctaaggtctt gacctatgct
    28021 atcttggtca ttgcccttgt tacttttgta gtgggtgtct tcattcaagg gaaaaatcca
    28081 cttggtgagt tgttgacttc tgttgccctt gctgttgcag ccattccaga aggacttcct
    28141 gctatcgtta ccatcgttct ttctcttggt actcaagttt tggccaaacg acattccatc
    28201 gttcgtaagt tgccagcagt tgaaacactt ggttcaactg aaatcatcgc ttctgataag
    28261 actggtacgc tgactatgaa caagatgaca gtcgaaaaag tcttctacga tgcggttcta
    28321 catgactcag ctgatgatat tgaactaggt cttgaaatgc cactacttcg ttcagttgtc
    28381 ttggccaatg atacgaaaat cgatgtggaa ggtaacttga ttggtgaccc aaccgaaaca
    28441 gcctttatcc aatatgcctt ggacaagggc tatgatgtca aaggtttctt agagaaatat
    28501 cctcgtgtag ctgaattgcc atttgactct gaccgtaagc tcatgtcaac agttcaccca
    28561 ttgccagatg gtcgtttcct tgtagcagtc aagggtgcgc cagaccaact cttaaaacgt
    28621 tgtcttcttc gtgataaggc tggggatatt gctccgattg atgagaaggt tacaaatctc
    28681 attcatacaa acaattctga aatggctcat caagccttgc gtgtccttgc aggtgcttat
    28741 aagattatcg atagtattcc agaaaatctc acttctgaag agcttgaaaa tgatttaatt
    28801 tttactggtt tgattgggat gattgatcct gaacgtcctg aagctgctga ggctgttcgt
    28861 gtggctaagg aagctggaat tcgtccaatt atgattacag gtgaccacca agacaccgca
    28921 gaagctattg ccaaacgttt gggaatcatt gacgcaaacg atacagaaga ccacgtttta
    28981 actggtgctg agctcaacga attatctgat gaagactttg aaaaagttgt tggtcaatac
    29041 tctgtttatg cccgtgtgtc tccagagcac aaggttcgta tcgtcaaggc ttggcaaaaa
    29101 caaggtaagg tcgttgccat gacaggtgac ggtgtcaatg atgccccagc tctgaaaaca
    29161 gcagacatcg gtatcggtat gggaatcact ggtacagagg tttctaaggg ggcttctgat
    29221 atgattcttg cagatgataa ctttgcgact attatcgtcg cagtggaaga aggacgtaag
    29281 gtcttctcaa atattcaaaa gactattcag tacctacttt ctgccaatac tgctgaagta
    29341 ttaaccatct tcctatcaac cttgtttggt tgggacgtct tgcagccagt tcatcttttg
    29401 tggattaact tggtaacgga taccttccca gcaattgcac taggtgttga acctgcggaa
    29461 cctggtgtca tgaatcataa accacgtgga cgcaaggcaa gcttcttctc aggtggtgtt
    29521 ttgagttcta tcatttatca aggtgtactc caagcagctc ttgttatgag tgtttatggc
    29581 cttgcgattg cttacccagt tcatgtgggt gacaatcatg ctattcatgc agatgcccta
    29641 acgatggcct ttgcaaccct tggtttgatt cagctcttcc atgcctacaa tgtcaagtct
    29701 gtctaccaat ccatcttgac agttggccca ttcaagtcta agacctttaa ctggtccatc
    29761 ttggtatcct ttatccttct catggcaaca atcgttgtag aaccgcttga aggaatcttc
    29821 cacgtaacca aactagactt gtcacaatgg ggaattgtta tggctggaag cttctcaatg
    29881 attatcatcg tcgaaatcgt taagtttatt caacgcaaac tcggttttga caagaatgcg
    29941 atttaaggga ggtttcggat gtcagcatat caattaccga ccgtatggca ggatgaagct
    30001 agtaatcaag gagcttttac ggggctaaac agaccaacag taggtgcccg tttcgaacaa
    30061 aacttgccaa aaggagaaca agcttttcag ctttattcac tgggaacacc aaatggtgtg
    30121 aaggttacta tcttattgga agaattacta gaagctggtt ttaaggaagc ggcttacgac
    30181 ttgtataaga ttgctatcat ggatggggat caattcggat cagactttgt gaagctcaat
    30241 ccaaattcca agattccagc cttattggac cagtcaggta ctgaagatgt aagagtcttt
    30301 gagtctgctc atattcttct ttaccttgct gagaaatttg gagccttttt accaagtaat
    30361 cctgtggaaa aggtagaagt tttgaattgg ctattctggc aagcaggtgc agcacctttt
    30421 ctaggtgggg gatttggaca tttcttcaat tatgctcctg aaaaattgga atatcctatt
    30481 aaccgtttta caatggaagt gaaacgccag ttggatttat tggataagga attggctcag
    30541 aaaccttata ttgcaggcaa tgactatacg attgcagata ttgctatctg gtcttggtat
    30601 ggacagttag ttcaaggaaa tctttaccaa ggttctgcaa aattcttgga tgcctcaagt
    30661 tatcaaaatc tagtaaaatg ggcagaaaaa attgccaatc gtccagctgt taagcgtggc
    30721 ttggaagtaa cttatacaga aattaaatag caaaaaagtc atcttcggat ggctttttgc
    30781 tacagttgag ggaaagggtt tgcagttatc ggcttttgtg ctataattgc tataatatag
    30841 taaaaagcaa gaggagtgtg aggaagtgga aaagaaaatt gtgaaggata tcttattttt
    30901 atctcaagtg tctcagccgg caagtcagga ggacctttat cttgccagag atttgcagga
    30961 tacactctta gcaaatcatg atacctgtgt tggtctagct gccaatatga ttggggtgca
    31021 gaagcgcgtg attatcttta atcttggctt ggttcccgtg gtcatgttta acccagtgct
    31081 tctgtccttt gaaggatcct atgaggcaga agaaggctgt ttgtccttgg taggcgtgag
    31141 atcaactaag cgttatgaaa ccataagggt tgcctatcgt gacagcaagt ggcaggaaca
    31201 gaccattacc ttgacaggct tcccagctca gatttgccag catgagctgg atcacttgga
    31261 aggacgaatc atttaggagg aaagcaaatg aaacgaatag tctttgaact tatttttatc
    31321 gcaacgacct ggtatatctt tttaccgccc cttaacctga ccagctggga atttctcttc
    31381 ttcctctgtg ggcatttgtt agttgtggca atcttatttg gctttggcaa ggggataaac
    31441 cttgtcaaaa cggttcatgt gcgccacggt aaggcggaag ctgccttaaa tcttgagggt
    31501 ttcaaaatca atcggttagg gaaaattctg ttagcttcga ttggaggaat tcttctcttg
    31561 gcagctttgg tttccttggt aacttccagc atgtttcagg ctaaaaatta tgccaatgta
    31621 gtcacggtta cggaaaaaga ctttactgaa tttcctagga gtgacaccag taaggttcct
    31681 atcctagata gaagtactgc tgaaaaaatt ggagaccgct acttgggttc cctaaccgat
    31741 aaggtgtcgc aatacgtagc ggcagatacc tatacccaat tgacaattga tgggaaacct
    31801 tatcgggtca caccactaga atatgcagac cctatcaaat ggtttaacaa tcaagccaag
    31861 ggaatcggtg agtatatcaa ggtggacatg gtaactggaa atgcggattt ggtggacttg
    31921 aagacaccaa tcaagtattc agactcggag tattttaacc gtgatgtcaa acgtcacctg
    31981 cgcttgaagt acccgaccaa aatctttaaa actccatctt ttgaggtgga cgatgagggc
    32041 aatcctttct atgtagcaac ggtttaccaa aagcaatttg gacttgctgt tcctcgtcct
    32101 gcttcagtca ttatcttgga tgctacaaat ggagaaacca aggaatacag cttatcagat
    32161 gttccagaat gggtggacag gatctatcca gcagaggaaa ccattgagca aatcaactac
    32221 aacggcaagt acaaggacgg tttcttgaat gccatgattt ccaagaaaaa cgtgacccag
    32281 actaccaagg gctataatta cttgtctatc ggtaatgaca tctatctcta cacaggtgtg
    32341 acgtcggcta atgcggatga gagtaatctt ggtttcatcc ttgaaaatat gcgaacagga
    32401 gaaatcacta agtatagctt ggcttctgcg acagaagaat cagcccgtga atcagcagaa
    32461 ggtgctgttc aggagaaatc ctacaaagca accttcccaa tcctcatcaa cctcaatgac
    32521 aagcctctct acatcatggg cttgaaggac aatgctggct tggtcaaaga gtacgccctg
    32581 gtagacgcag tcgagtacca aaatgttatc gttgctacta cagtggaaga gatgctcagc
    32641 aagtatgcca ataaaaacga ccttgaaatt gacaatgcaa cgacagaaag catcaatgga
    32701 gtagtagcag acctcaaatc agctgttatc aagggagaca ctgtctactt ctttaaagtt
    32761 gatggcaaga tatacaaggt caaggcttca gtatccgatg accttcctta ccttgaaaat
    32821 ggtaaaacct tcgaaggtca agtaggaaaa gacaattatc tcaagacctt taagctacgg
    32881 taaaaatagg tttttttcag aaagtatatg ttataataag gtaaattaag cctaatggag
    32941 gacaaagaaa tgggttttta tttgatggtt gcttcaatgc tactcggact gctcgctttg
    33001 aagataggtt tttctcagtt caaggagaaa aaagataaat tcttatctat cttaacaagt
    33061 ttagctggca cagcccttgt tctcgtggct gtctggttag gatggcccaa ataaatagaa
    33121 aaacctcggt caaaccgagg tttttcaaat gaatttcttg gttgtggcta aaaaatatgc
    33181 tacactatca atatgaaaat tttaatccca acagcaaaag aaatgaacac agacttgcca
    33241 agtatcgagg caatttcttt aaaaccagaa agtcaggccg tgcttgatgc cttggctctc
    33301 tattctgcca gtcaattgga gagtttctac aaggtatcag ctgagaaagc ggcggaagaa
    33361 tttcaaaata tccaagcttt gaaaaggcaa actactcaac actatccagc cttgaaactt
    33421 tttgatgggc ttatgtaccg caacattaag agagataagc tgaccgaggc ggaacaagat
    33481 tatcttgaaa atcatgtctt cattacctcg gctttgtacg gtgttgttcc agccttgtca
    33541 cccatggctc ctcaccgttt ggattttttg atgaaattaa aagttgctgg taagactttg
    33601 aagagccatt ggaaggcagt ctatgatgaa gctctgaaga aggaagaagt gattttctct
    33661 ctcttgtcat cagagtttga gactgtattt tctaaggaaa tcagagcaaa gatggtgacc
    33721 ttcaaattca tggaggatag aggcggtcag ctgaagattc actcaactat ctccaagaaa
    33781 gcgcgcgggg cctttctaac agctttaata gaaaatcaag tacaaactgt gggggaagca
    33841 cgtcgcttga actttgctgg atttgtttac cgagaagatt tgtcacaacc acagggattg
    33901 gtttttgtaa aggaagtata gaaatcagat attttgtatt tggcaatatg tatcatcagc
    33961 tattttagtt tataaatgag aaaaaagcga acaagctagt cttctgagcg tcagaaaagt
    34021 agtcttgttc gcttttatta tgttatactc ttcgaaaatc tcttcaagcc atgtcagtgt
    34081 cgccttaccg tatatatgtt actgacttcg tcagttctat ctacaacctc aaagcagtgc
    34141 tttgagtagc ttgcggctag cttcctagtt tgctttttga tttttattga gtgttagtta
    34201 cttgatagct tcgaccaagt cttgagcttg tttttcaagt gagtttagga ctgtttcttc
    34261 aagaaccaat tttccgtctg cccaggcaga gtcattaaca cgtgcagcag tgaaatcacc
    34321 aacgccttgt gtacggataa atggcaagag gtctttgtag atagcgaaaa gttgatcgtg
    34381 ccctgcattg gctacagatg atactgtgac aaacttgtct tgaagggcag aaacgccacg
    34441 tgtatcagac aagtcaaggg cacgagatag ccagtcaagc aagtttttca ctgtaccagg
    34501 gatagagaag ttgtagactg gagagaaaat ccagatagca tccgcaacga gaactgcttc
    34561 acgagcagca gctacagctg gatgtgttgg aacttccaaa tcttggctaa agagaggaag
    34621 ggctgaataa tcaaggtagc taacttccgc tttaccagca agtgctttct cagcttcgag
    34681 cgccatttgg tggttgaaag aaccttgacg tagtgatccg acgataaata atactttttt
    34741 agacatgatg tgtctccttt ttcttaaaat ttcgagaact ctctcgttgt tataaaatat
    34801 attacaacaa aacaaaagaa cttgcaagaa atttgctcag agtttatttg aaaattgtaa
    34861 aatttcggaa actctaatca tttgtgactg aaaaagtaag aactgtttca ctttcaaaat
    34921 cggttttgtt tgaagggaga aatagaattg gttgctcaaa gcacagcttt gaggttgcag
    34981 ataaagctga cgtggtttga agagattttc gaagagtatt cttatattca cttgtaataa
    35041 agcttaatag atggtaaaat agacgagtga aaaaaagaca aaacaaagca aaaaataatc
    35101 tactgtggca gtatggtcta gggatgacga ttttgtttgt ggttatcagt gcttcctttc
    35161 tgtatatggt ttctcttagc atgaaaccct atcaaacagc taaaagtgaa ggagaaaaat
    35221 tagctcagca gtatgcagga ttagagcagg tcgatcaggt tgatttatac aatggcttgg
    35281 aatcttatta cagcgttctt ggtcgtaata aacagcaaga agcgcttgct gtcctgattg
    35341 gtaaagatga tcataagatt tacgtttatc agctaaatca gggtgtttca caagaaaaag
    35401 cagaaacggt ttctaaggaa aagggagctg gcgaaattga caagattatc tttggtcgtt
    35461 atcaagataa gccaatctgg gaagtcaagt caggatctga tttttatcta gtagattttg
    35521 aaacaggagc attggtcaac aaggagggcc tatgaaatta tccaaccgtg ttttagaaat
    35581 ggaagaaagt gtgactttgg ctgctggagc ccgtgccaaa gcactgaagg ctgagggtag
    35641 agatattttg tctctaacct tgggtgagcc agatttcact actcccaaaa atatccaaga
    35701 tgccgccatt gcatcgattc gagatgggcg tgcttctttt tatacagtaa cctcaggtct
    35761 gccagagctt aaggcggcgg tcaatagcta ctttgagcgc ttttacggct attctgtagc
    35821 gtcaaatcaa gtgacagtcg ctgcgggagc caaatattct ctctatacct tctttatggc
    35881 tgtggtcaat ccaggtgatg aagtcatcat cccaacccca tactgggtta gctatggaga
    35941 tcaggtcaag atggcagaag gcgttcccgt ctttgtttct gctaaggaag acaatcactt
    36001 taaggtgacc gtagagcagt tagaagcagc tcgcactgac aagaccaagg ttttggtgct
    36061 gaattcgcca tctaatccca caggtatgat ttacacccgt gaggaactct tggcaattgg
    36121 aaactgggct gtagaaaatg atattctcat cctagcagac gatatctatg gccgcttggt
    36181 ttataatggt catgagttca caccgatttc tagcctatcg gaagcgattc gcaagcaaac
    36241 agtggtcatc aatggtgtgt ctaaaactta tgccatgact ggttggcgga ttggttatgc
    36301 cgttggagaa gcagatatta tcgctgccat gtccaagatt gcaggtcaaa caacttcgaa
    36361 tccgtcagca gtagcccaat atgcagcagt tgaggctcta tcaggtgagc aagatactgt
    36421 agaaagcatg cgtcaggcct ttgaggaacg tcttaatact atctatcccc tccttgcaga
    36481 ggtgccagga tttgaagtgg tcaaaccgca aggggccttc tatctcttcc caaatgtcaa
    36541 aaaggccatg gagatgaaag gctacacgga tgtgacagac tttacaactg ctatcttaga
    36601 agaagccgaa gtggccttgg taacaggagc tggatttgga gcaccagaaa atgtccgcct
    36661 cagctatgcg acagacctag acacgcttaa agaagcagtc gaacgcttga aagcatttat
    36721 gggtagtgag aatgattgat cattttgaga ttaaggtaaa ggatttacaa atttcagaag
    36781 gattttatag gagttttctc gctcctttgg actataaatt ggcttttaag actagttctc
    36841 taattagttt tctatccccg aacagccctc atcctggtgg tgatttttgg ctgactcagg
    36901 gaacacaaga tcctgttcat tttgcttttt tagcagaaaa taaggaagaa gttcaggctt
    36961 gttatgaggc tggcttagag gcaggtgggc gagacaatgg ggctcctggt tatcgaagtg
    37021 agcgtctgat ttactatgct gcttttatga ttgacctgga tgggaacaat atagaggtgg
    37081 tttgccataa agaataaaaa agaaaaggaa aaataatgac aaaacgtgta acgattattg
    37141 acgtaaaaga ctatgttggt caggaagtga cgattggcgc ttgggttgcc aacaaatcag
    37201 gaaaaggaaa aatcgctttc ttacaattgc gtgatggaac agccttcttt caaggtgtgg
    37261 cttttaaacc aaactttgtc gaaaaatttg gtgaagaagt gggacttgag aagtttgatg
    37321 ttatcaaacg cttgagccaa gaaacgtctg tttatgtgac aggtattgtc aaagaggacg
    37381 aacgttctaa atttggctat gagttggaca tcacagacat cgaagtgatc ggtgaatctc
    37441 aagactaccc aatcacacca aaagaacacg gaacagactt tttgatggat aaccgtcact
    37501 tgtggctacg ctctcgtaag caagtagctg tgttgcaaat ccgtaacgct attatctatg
    37561 caacttatga gttctttgac aagaacggtt ttatgaagtt tgacagccca attctttcag
    37621 gaaatgcggc agaagattct acagaactct ttgaaactga ctacttcgga acgccagcct
    37681 acttgagcca atcaggtcag ctttacctag aagcaggggc tatggctctt ggtcgtgtct
    37741 ttgactttgg tccagttttc cgtgctgaaa aatcaaaaac acgccgtcac ttgactgagt
    37801 tctggatgat ggatgctgag tactcatact tgacacatga tgagtcgctt gacttgcaag
    37861 aagcttatgt gaaagctctt ctacaaggtg ttcttgaccg cgcgcctcaa gccttggaaa
    37921 ccttggaacg tgatacagaa ctcttgaaac gctacattgc agagccattc aaacgtatca
    37981 cttacgatca agccattgac ctcttgcaag agcatgaaaa tgatgaagat gctgactacg
    38041 agcatcttga gcatggtgat gactttgggt caccacacga aacttggatt tcaaaccact
    38101 ttggtgtgcc aacatttgtc atgaactatc cagcagccat caaggccttc tacatgaaac
    38161 cagttcctgg aaatccagag cgcgtgcttt gtgcagactt gcttgctcca gaaggctatg
    38221 gagaaattat cggtgggtct atgcgtgagg aagattacga tgcccttgtc gctaagatgg
    38281 atgaacttgg catggatcgt acagaatatg aattctacct tgaccttcgt aaatacggta
    38341 cagttccaca cggaggattt ggtatcggta tcgaacgtat ggtaaccttc gcagcaggaa
    38401 caaaacatat ccgtgaagct attccattcc cacgtatgtt gcaccgtatc aaaccataag
    38461 aacaggaaaa cgcccatgtg gcgttttttc gaattctagt gtttttactt gcgtaaaaaa
    38521 attttttcta gtataatagt ttattgtgag cgaacctcac ttaccccttg caaagtcttg
    38581 gggtcattag accaaaagga ggaacatatc aatggctaaa tacgaaattc tttatatcat
    38641 tcgtccaaac attgaagaag aagctaaaaa cgctttggta gcacgttttg actctatttt
    38701 gactgacaac ggtgcaactg ttgttgaatc aaaaacttgg gaaaaacgtc gtcttgcata
    38761 cgaaatccaa gatttccgtg aaggacttta ccacatcgtt aacgttgaag caaatgacga
    38821 tgcagctctt aaagagtttg accgtctttc aaaaatcaac gctgacattc ttcgtcacat
    38881 gatcgtcaaa attgacgcgt aagaaggtaa actatgatta acaatgttgt acttgtaggg
    38941 cgtatgacac gtgacgctga gttgcgttat accccatcaa atgtagcagt tgcgactttt
    39001 actcttgcag taaaccgtac atttaagagt caaaatggtg aacgtgaggc tgattttatc
    39061 aatgtcgtta tgtggcgcca acaggctgaa aatcttgcta actgggctaa aaaaggctca
    39121 cttatcgggg tgacaggtcg tatccagact cgtagttacg ataaccagca aggacaacgt
    39181 gtctacgtga cagaggtcgt ggctgagaat ttccaaatgt tggaaagccg tagtgtgcgt
    39241 gagggccaca caggtggagc ttactctgca ccaactgcaa actattcagc acctacaaat
    39301 tcagtaccag acttttcacg taatgaaaat ccatttggag caacaaaccc attggatatt
    39361 tcagatgatg atttaccatt ctaatggaca actaaactta taaaggagaa aaaacatggc
    39421 tcaacaacgt cgtggcggat tcaaacgccg taaaaaagtt gattacatcg cagcaaacaa
    39481 aattgaatat gttgattaca aagatactga gcttcttagc cgtttcgttt cagaacgtgg
    39541 gaaaatcctt cctcgtcgtg taacaggaac ttcagctaaa aaccaacgta aagtaacaac
    39601 agctatcaaa cgcgctcgcg taatggcttt gatgcctttc gtaaacgaag attaaagaaa
    39661 agacccggtt gggtctcttt ttcatgagct tgaaaacaag aaagcgaatt aagaccccaa
    39721 cgggtctttt ttcacaagct ttgaaatgag agagcgagtt aggctcagtg gctccctttt
    39781 tacgagtttg aaaacaagag aacacattaa aagcacgatg gagctttttc ataaatatgt
    39841 cttttttctc atccaagaaa ttaaaataaa aatgataaac tttttgaaaa taggggattt
    39901 gcctacttta tgatataatg gaaagagtta gattgaaaga ggtagtgaga tggacgcgaa
    39961 attaagatac aaggcaaaga agatcaagat tgtctttttt gatattgatg atacattgcg
    40021 gaattcaaag acagggttta ttccaactac aattcccact gtttttaaac agttgcgtga
    40081 aaaaggaatt ttaacaggaa tcgcctctgg acgtggcatt tttggtgttg ttccagagat
    40141 tcgtgatctc aagcctgact tttttgtaac tttgaatgga gcttatatcg aagataaaaa
    40201 aggtcaggtc atttatcagc atcagattga aaagtcagat gttgaggagt atatctcttg
    40261 ggctaagcaa gaaggaattg agtatggctt ggttgggagt catgatgcca agttgtcgac
    40321 tcgcaccgat atgatgagtg aagctatcaa tccaatttat cccgacttag atgtagatcc
    40381 cgatttccat gaaaaagaag atatctatca gatgtggact tttgaagata agggagatga
    40441 cttgcacttg cctgacagtc tctcagacaa acttcgcatg gttcgttggc atcaacattc
    40501 gtctgatatt gtgccgattt caggctccaa agcgacgggg gtggaaaagg ttgtggaaca
    40561 ccttggcttg aaaccagaga aggtcatggt ttttggagat ggtctcaacg acttggaact
    40621 ctttgattat gctggaatca gcgttgccat gggaatttct catgataaaa tcaaagaaaa
    40681 agcagattat attacaaaaa cattagaaga agatggcatt tttgatgcct tagaagtatt
    40741 tggtatggta gaaaaagaat tgcattttcc acaagtagac attgaaacag tagaaggtcc
    40801 tcttgcgact attaagacca atcacggaga cttacgtatc aagcttttcc ctgaacatgc
    40861 tcctaaaaca gtggctaact ttgtatctct ttcaaaagat ggctactatg atggtgtcat
    40921 tttccaccgt attatcaagg actttatgat ccaaggtgga gacccaactg gaactggtat
    40981 gggtggcgag tcaatctacg gcgaatcttt tgaggatgaa ttctcagaag agctttacaa
    41041 tatccgtggt gctctttcca tggcaaatgc tggtccaaat accaacggca gccagttctt
    41101 tatcgtgcaa aaccaacacc taccttattc gaagaaagaa attactcgtg gtggttggcc
    41161 agaaccgatt gcagaaatct atgccaatca aggtgggaca cctcacctag accgccgtca
    41221 cacggttttt ggtcagttag ctgatgaagc atcttacgct gtcttggatg ccattgctgc
    41281 tgttgagaca ggagctatgg acaagccagt tgaagatgtt gtaattgaaa ctattgaaat
    41341 cgaggactaa gatgaaaatc ggtgataagc taaaggggcg tattacaggg attcagccct
    41401 acggtgcctt tgttgagtta gagacgggtg atacggggct gattcacatt tcagaaattc
    41461 ggacaggatt tattgaaaat attcatgaaa ccttgaaagt cgatgaagag gttcaagttc
    41521 aggtagtgga tttagatgaa tttacgggga aagccagtct ttctatccgc actttggagg
    41581 aagaaaagta ccagtttcct agacggagac gcttttcaag cgaccgcttt aactacggtt
    41641 ttgctccctt tcgacgaatg ctaccaatct ggactggaga agccctgcac catttaaaaa
    41701 agaagaagta gtttagagtt ttatattttt tgtaatgtta atatgaattt gctataatag
    41761 gaccatggaa gaagaacaat tattaaaatc aggagagcgc attaaccagc tcttttcgac
    41821 agatatcaaa atcattcaaa atagagaggt ttttagctat tcggtggata gtgttctctt
    41881 atcacgattt ccacgttttc ctaagaaggg gttgattgtg gatttctgtg ctgggaatgg
    41941 agcagtgggg ctttttgcta gcactcgtac tcaagcacag atattgtctg ttgagattca
    42001 ggagcgtttg gcggatatgg ctgaacgctc tgtccgtttg aatggattag aggagcaaat
    42061 gcaggtcatc tgcgatgatt tgaaaaatat gcctgctcac attcagggga gtaaggtgga
    42121 tatgattttg tgtaatccac cctatttcaa ggtgaatcct tattctaatc tgaacgagag
    42181 tgaacattat ctcttggctc gacacgaaat cacgactaat ttggaagaaa tctgtcgtag
    42241 tgcccagagt attctcaagt ctaatgggcg tttggccatg gttcatcgtc ctgatcgact
    42301 tctggatatt ttggatacgt taaaacgaca taatctagcc cctaagcgcc tgcagtttgt
    42361 ttatccaaaa agagaaaagg aagccaatat gcttttgatt gaggcgatca aggatggctc
    42421 aacaagtggc tttaaggtct tacctccttt gattgtccac aatgatgatg gctcttatac
    42481 gcccgaaatc gaagagattt attatggatc ataaggctta tatgtacgtt ctggagtgtc
    42541 gtgatggatc ctactatata ggctatacga ctgatatgag aagacgcctc gctatccaca
    42601 atagtgggaa gggagccaaa tatacacgag cacgcttgcc agtcaaactt atctatgctc
    42661 aaggttttgc cagtaaggaa gaagccatgt cggctgaagc tcttctcaag cgtaagaaga
    42721 ggccacagaa ggaagaattt ttatctgaaa atcaagatag aaatttactc agttattttg
    42781 aagaatcgtg gggagtcctt tgactcctct tacctttgtc aaaaagcgaa aaaaatgcta
    42841 caataaagaa aataaacaaa atgaggtatt atcatgtcca agattctagt atttggtcac
    42901 caaaatccag actcagatgc catcggatca tctgtagctt ttgcctacct tgcaaaagaa
    42961 gcttacggtt tggatacgga agctgttgcc cttggaactc caaatgaaga aacagccttt
    43021 gtcttgaact attttggtgt ggaagcacca cgtgttatca cttctgccaa agcagagggg
    43081 gcagagcaag ttatcttgac tgaccacaat gaattccaac aatctgtatc agatatcgct
    43141 gaagtagaag tttacggtgt tgtagaccac caccgtgtgg ctaactttga aactgcaagc
    43201 ccactttaca tgcgtttgga gccagttgga tcagcgtctt caatcgttta ccgtatgttc
    43261 aaagaacatg gtgtagctgt gcctaaagag attgcaggtt tgatgctttc aggtttgatt
    43321 tcagataccc ttcttttgaa atcaccaaca acacacccaa cagataaaat cattgctcct
    43381 gaattggctg aattggctgg tgtgaacttg gaagaatatg gtttggcaat gttgaaagct
    43441 ggtaccaact tggctagcaa atctgctgaa gaattgattg atatcgatgc taagactttt
    43501 gaactcaacg gaaataatgt ccgtgttgcc caagtgaaca cagttgacat cgctgaagtt
    43561 ttggaacgcc aagcagaaat tgaagctgca atgcaagctg ccaacgaatc aaacggctac
    43621 tctgactttg tcttgatgat tacagatatc gtcaactcaa actcagaaat cttggctctt
    43681 ggtgccaata tggacaaggt cgaagcggct ttcaacttca aacttgaaaa caatcatgcc
    43741 ttccttgctg gtgccgtttc acgtaagaaa caagtggtac ctcaattgac tgaaagcttt
    43801 aatgcgtaag attttgggtg tcagctcaaa atcggaaagt ctagtttgcc ttatatcgaa
    43861 aggagtttcg gctccttttt tctaggagtg aagtatgtta gaaaatggcg atttgatttt
    43921 tgtgagagat gggtcagaca tgggacaggc catccagact tccacaggta actatagcca
    43981 tgttgccatt tatttggatg ggatgattta tcatgctagt ggacaggctg gtgttgtctg
    44041 tcaagaaccg gcagacttct ttgagtccaa tcatttatac gacctctatg tttacccaga
    44101 aatggatatc cagtcggtga aggaaagagc ttgcaaacat cttggagcac cctacaatgc
    44161 ttctttctat ccagatgcac ctggtttcta ctgctcccag tatatagcag aaatcctacc
    44221 tatttttgaa actattccta tgaaatttgg agatggggag caggcgatta gtgatttttg
    44281 gagggagtat tacatagaac taggtctgcc tgttcctctg aaccaagctg gtaccaatcc
    44341 tagtcagttg gcagcatcgc ctctgttaca atgtaaagaa aggaatcttc atgattcaga
    44401 tttttaatcc atctcgtttg acgagacagc catttttgga gaattgatcc gctatctgga
    44461 ccagtatgag gatgtgattc tacgggaaat taaggctcaa tttccagatg ttgcagttga
    44521 taaactcatg gaagagtata taaaggcagg cttgattcta cgtgaaaata agcgctatta
    44581 cctcaatttt cctacgcttg aatcacttga tagtcttgaa ctggatcaag agatttttgt
    44641 cagagaagct agtccggtct atcaagcctt gttggagcag agttttgaga cggaattgcg
    44701 caatcaaatc aatgcagcta ttttagttga aaagacggac tttgcgcgca ttaaaatgac
    44761 tctgtccaat tatttttaca aggtcaaaca gcagtatcct ttgacagaaa aacagcagga
    44821 gctctatgac attttaggag atgttaatcc tgagtatgca ctcaagtata tgacggcttt
    44881 tttgttgaaa tttctcaaaa aagaccagct tatgcagaaa tgccgtgata tctttgtgga
    44941 cagtttggtt gtcttaggct atattgtgca aaatgaagat ggaaagtatg agttggctat
    45001 cgattttgat aaggagagat taactttcta cttagcgtga tttcttgttt ctgagtacat
    45061 tgtttgactt tccttagtat tcggtataaa ctatatgtaa ccggtaacac atatcggaat
    45121 aaactaaagg agacaatcat atgtcacttg aaaacaaatt ggaacaagca acaggcgctg
    45181 tcaaagaagg ttttggtaaa gttactggag acagcaagac agaacttgaa ggagctgttg
    45241 aaaaaacagt tgctaaggca aaagacgttg tagaagacgc aaaaggtgct gtagaaggtg
    45301 ccgttgaagg tttgaaaaac gtttttacta aagaatagga aaaaatcaag ggtttcattt
    45361 tcccttgatt ttttctattc ttataaataa ttttctgcga cggctgtatc tcctgggtag
    45421 gattctttct tgccctggat gatttggtaa caatcggctc ccttacccgc aataataact
    45481 gcatctaatt cgtgatttgt gatagccatt gccgccttga tggcttcttg gcgatccgca
    45541 atcttctcaa caggatgatt gatgtagcta ctaatttcat ctgcaatggc cattgggtct
    45601 tcatagttag ggtcatcagc agtcagaaag acttgaatct cagggtgttg attgaggagg
    45661 aggccaaagt ccttacgacg actttctccc ttgtttcctg ttgatcccag aaccagagca
    45721 atctttccgg tttgatgagt ttcaaccaca ttgatgagtt ttttcagact atccccattg
    45781 tgggcatagt cgatgaagac cttggctcca tttttctgag tgaggacttc catacgacca
    45841 ggaacgcggg ttgcagcgat gccttttttg atgtcctcaa gacttgctcc gagacggaga
    45901 caagcaagtc cagcagcaac tgcattttct tggttgaagt tgccaatgag ttggatatca
    45961 taatctccag cgagtttacc cgtagctgaa aagctaaagg ctttggaatt ctcgatttgg
    46021 ttatcaaatt ggctaccata gaaatcatgg tcttgatctt caacctgttc tttcaagact
    46081 gagaagtggt ccatgtcact gttaatgatg actgctcggc tattttccat caagagacgc
    46141 ttgtggtaga aatagtcttc aaagctaggg tgttcaatcg ggccgatatg gtctgggctg
    46201 atattgagga aaactcccac atcaaaggtt agaccataga cacgtctgac cagataggat
    46261 tgactggaga cttccatgat gaggtgggta cggtcatttt gcacagcctg attcatcatg
    46321 tcaaagaggt caatactctc aggggttgtc aacgctgact taaagaaagt ctcgccatca
    46381 agagttgtgt tcatggtcga caacatagca ggtcgatgcc cttgagataa gatgttatag
    46441 gcgaaatagg ctgctgttgt cttaccctta gtaccagtaa aggcaaggag tttgagtttt
    46501 tcctgtggat taccatagaa ctccatggca atcaaactca tggctttctt tatatcgttc
    46561 acaatgatga cagggatacc gacttcgtag tccttttcag ctacatacca agctaagcct
    46621 tgtgttatag cagaaagaag gtattctttt ttaaaggcag cgccttttgc aaaaaaaaga
    46681 gtgtcttctg ttacttttcg gctatcgtag ctgatgctat caaaaacaac tttgctgtag
    46741 ttgtagtggt aatgaccttg gtcaataatt tcgcgaaaaa ggccatcttt ctttaaaata
    46801 tctaatacag tttcaatttt tatcatactt tctattgtaa accgaaagtc gcaaatttac
    46861 aagtaacaag gaaaagttta taatggaaga taaggagttt ttcctagtta tcaaaattga
    46921 atgaggaatc tatgtcgcac gaaaacaatc accagcaggc ccagatgtta cgggggactg
    46981 cttggctaac ggctagtaac tttatcagtc gcctactcgg ggctgtttac attatccctt
    47041 ggtacatctg gatgggggct tatgcagcta aggcaaatgg tctctttacc atgggttaca
    47101 atatctatgc ttggttcttg ttggtttcaa cagcggggat tccagttgcg gtggccaagc
    47161 aagttgccaa gtataatacc atgcgagaag aagagcatag ctttgccctg attcggagct
    47221 tcttaggctt tatgacagga ctaggcctgg tttttgcttt agtcttgtat gtctttgctc
    47281 cttggctagc agacttgtct ggcgtgggca aagacttgat cccaatcatg caaagcttgg
    47341 cttggggagt cttgattttc ccgtctatga gtgttatccg aggatttttc caagggatga
    47401 ataacctcaa accttatgcc atgagccaaa ttgctgagca ggtcattcgt gttatctgga
    47461 tgctcctagc aacctttatc attatgaagc tcggttcagg agattatcta gcagccgtta
    47521 cccaatcaac ctttgctgcc tttgtcggta tggtagccag ttttgcagtc ttgatttatt
    47581 tccttgccca agaaggttca ctcaaaagag tctttgaaac aggagataag attaacagta
    47641 agcgtctctt ggttgatacc attaaggaag ccattccttt tatcctgaca gggtctgcca
    47701 tccagctctt ccagattttg gatcagctga cctttatcaa tagtatgagc tggtttacca
    47761 actacagcaa tgaggacttg gttgtcatgt tttcttattt ctcagccaat cctaataaaa
    47821 tcacgatgat tttgatttct gtaggggttt cgattgggag tgttggtttg ccacttttga
    47881 cggaaaacta tgtcaagggg gacttgaaag cagcttctcg tctggttcag gacagtctca
    47941 ccctactctt tatgttcttg ctaccagcaa cggttggagt ggttatggta ggagaacctc
    48001 tatatacagt cttctatggt aagccagata gtttggctct gggcttattt gtctttgcag
    48061 ttttgcagtc tattatttta ggcttgtaca tggtcttgtc tccaatgctt caggccatgt
    48121 tccgcaaccg caaggccgtt ctctatttta tctatggttc tattgccaag ctagtcttgc
    48181 aactacctac catcgccctc ttccacagtt atggtccttt gatttcaaca accattgctc
    48241 tcatcattcc taacgtcttg atgtatcggg atatttgtaa agtaactggt gtcaagcgca
    48301 aggtgatttt gaagcgaacc attttaatca gtttgctgac cctagtcatg tttctgttaa
    48361 taggaaccat ccagtggctg ttaggatttt tcttccaacc aagtggacgt ttgtggagct
    48421 tcttttatgt agctcttgtc ggtgccatgg ggggtggact ttatatggtt atgagtctgc
    48481 gtacctattt attagataag gtaataggaa aagcccaagc agatcgcctg cgagcaaaat
    48541 ttaagctttc gtaaggtagc atttataaat aaaaggaata agttgaacaa tcttttagga
    48601 gaacttgaaa cttattcctt ttttagcgtc aaaatagcta aaattatctt tcaaaaatag
    48661 aaaatgaggt ttttttgatt ttttggaaaa aacaattgac taaacaaacc aagagttgta
    48721 taataaaaca aatatttaaa gcaggaggtt ctggaaatga aaaagtctaa gagcaaatat
    48781 ctaaccttgg caggtcttgt cctgggtaca ggagttttat tgagcgcgtg tggaaattct
    48841 agcactgcat caaaaaccta taactatgtt tattcaagtg atccatctag tttgaactat
    48901 ctagcagaaa accgcgcagc aacatccgat attgttgcaa atttggtaga cgggttatta
    48961 gaaaatgacc aatatgggaa tattattcca tcattagcag aggattggac tgtttctcag
    49021 gacggtttga cctataccta caaacttcgt aaggatgcca agtggtttac ttctgaggga
    49081 gaagaatatg cgcctgtaac tgcccaggat tttgtgacag gtttgcaata tgcagctgat
    49141 aaaaaatcag aagctttgta tctagtgcag gactctgttg ctggtttgga tgactatatc
    49201 actggtaaaa caagcgactt ttcaactgtc ggtgtcaagg cacttgatga ccaaacggtt
    49261 caatatactt tggttaaacc agaactttac tggaattcaa aaacacttgc aacgatactt
    49321 tttcctgtta atgcagattt cctgaaatca aaaggggatg attttgggaa ggcggatcca
    49381 tctagtattt tgtacaatgg acctttcttg atgaaagcac ttgtctcaaa atctgctatt
    49441 gaatataaga aaaaccctaa ttactgggat gctaagaatg tctttgtaga cgatgtgaaa
    49501 ttgacctact atgatggtag cgaccaagaa tcactggaac gtaattttac agctggtgct
    49561 tatactacgg ctcgtctttt tcctaacagc tccagctatg aagggattaa agaaaaatac
    49621 aaaaacaata tcatctatag tatgcaaaat tcaacttcat atttctttaa ttttaaccta
    49681 gataggaagt cttacaatta tacttctaaa acaagtgaca ttgaaaagaa atcgacccag
    49741 gaagcagttc tcaataaaaa cttccgtcag gctatcaatt ttgcttttga cagaacatct
    49801 tatggggctc agtctgaagg gaaagaaggt gcaacaaaga ttttgcgtaa cctagtggtt
    49861 cctccaaact ttgtcagtat caagggaaaa gactttggtg aagttgtagc ctctaagatg
    49921 gtcaactatg gtaaggaatg gcaaggtatc aactttgcgg atggtcaaga cccttactac
    49981 aatcctgaga aagccaaggc taagtttgcg gaagctaaga aagaactcga agcaaagggt
    50041 gttcaattcc caatccactt ggataagact gtggaagtaa cagataaagt aggcatacaa
    50101 ggagttagtt ctatcaaaca atcaattgaa tctgttttag gttctgataa tgtagtgatt
    50161 gacattcagc aattaacatc agatgagttt gacagttcag gctactttgc tcaaacagct
    50221 gctcagaaag actatgattt atatcatggc ggttggggac ctgattatca agacccgtca
    50281 acctatctcg atatttttaa tactaatagt ggaggagttc tacaaaatct tggactagag
    50341 cctggagaag ccaatgacaa ggctaaggca gttggactgg atgtctatac tcaaatgttg
    50401 gaagaagcta ataaagagca agatccagcc aaacgttatg agaaatatgc tgatattcaa
    50461 gcttggttga ttgatagttc tttagttctt ccaagtgttt cgcgtggggg aacaccatca
    50521 ttgagaagaa ccgtaccatt tgctgctgcc tatggtttaa ccggtacaaa aggggttgaa
    50581 tcatataaat acctcaaagt acaagataag attgtcacaa cagacgaata tgcaaaagcc
    50641 agagaaaaat ggttgaaaga aaaagaagaa tccaataaaa aagcccaaga agaattggca
    50701 aaacatgtca aataaaatct aattgtagat aagtttgtgt aagaagtctt tgtgcttaaa
    50761 aacgcataat atcaggtata taaaatccta gatgttatgc gttttgttat aggaagattt
    50821 attagatttt atcctcaaat tgagttattc ccgactttat ccgttgaaat attataagga
    50881 gtatttatga aattaaaatt attaagagta gatactaagg tgattatggg gagtttctta
    50941 cttgttctgt ctagtctact tgctttgttg cttcccctta tcttaaaggg tttaatagat
    51001 gggagttcta ttgaaaatat aggttccaaa gtatttcaat cgtttttgat ttttattggt
    51061 caagccttgt tttcttctat tggttactat ctgtttagtc aatcgggtga aaaaaagata
    51121 gcaaaaatca ggaaaaaagt gatagagggg ttg
//
LOCUS       DAVKQL010000005        48174 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVKQL010000005 DAVKQL010000000
VERSION     DAVKQL010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 48174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48174
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.005"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(77..625)
                     /locus_tag="VW561_000224"
     CDS             complement(77..625)
                     /locus_tag="VW561_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEU9942564.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLVGYGLWWTWIIASGLFGLVVGLFRKYVRVINS
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(640..1488)
                     /locus_tag="VW561_000225"
     CDS             complement(640..1488)
                     /locus_tag="VW561_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEU9942565.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     DQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            1677..3026
                     /gene="trkA"
                     /locus_tag="VW561_000226"
     CDS             1677..3026
                     /gene="trkA"
                     /locus_tag="VW561_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEU9942566.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            3030..4469
                     /locus_tag="VW561_000227"
     CDS             3030..4469
                     /locus_tag="VW561_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU9942567.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(4595..6274)
                     /locus_tag="VW561_000228"
     CDS             complement(4595..6274)
                     /locus_tag="VW561_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9942568.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIAWMRKVYMLTMGVLGVIVSGTVAKSLAGNVNRKMPPGKV
                     INDTSAMLAAICGYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSAFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDTFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGAESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWTFPGPLGLLIGTNF
                     QLISFVFLSLILVVDILIYLPFCRAYDRQLLVKEDIASSNDIILEEDTSEIMPGEIDE
                     IKSKELKVLVLCAGSGTSAQLANAINEGANLTEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKSPSKALQFVLEHYQAV"
     gene            complement(6344..7756)
                     /gene="lacG"
                     /locus_tag="VW561_000229"
     CDS             complement(6344..7756)
                     /gene="lacG"
                     /locus_tag="VW561_000229"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEU9942569.1"
                     /translation="MENLQVKALPKEFLLGAATAAYQVEGTTRVDGKGTNMWDVYLQE
                     NSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSISWVRIFPDIDGEPNILAVRYYHR
                     VFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKIGC
                     IHALKPGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSDDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAPLNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
     gene            complement(7766..8110)
                     /locus_tag="VW561_000230"
     CDS             complement(7766..8110)
                     /locus_tag="VW561_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9942570.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEASGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            complement(8278..10164)
                     /locus_tag="VW561_000231"
     CDS             complement(8278..10164)
                     /locus_tag="VW561_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HEU9942571.1"
                     /translation="MVIRVFDQQKNTYSSFALEELSYYMNRVFKTNIELVEEKEADIF
                     VGLVNKEDRKDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRRHDFVP
                     ELRFEDFLDKQLSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEKFSNELVQELSDRLDKELQKRGLIHHRVGHGWTAEVLGY
                     SSKFGWESGLSISEEKKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYLRNKIILP
                     NSLEENLSYLFEWQKTFKGDSFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGEMLWKKTRSYEELIEEYFSALYGSNWQSVVEYLEKL
                     SSYSSCDYFNAIGSRQNDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            complement(10166..11488)
                     /locus_tag="VW561_000232"
     CDS             complement(10166..11488)
                     /locus_tag="VW561_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9942572.1"
                     /translation="MNNILAFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEAFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRMFIQRGITIKMPSGVPDVVSKSFSALLPGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTTENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
     gene            11656..12879
                     /locus_tag="VW561_000233"
     CDS             11656..12879
                     /locus_tag="VW561_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU9942573.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNNPIWESFNLLNVIKRFNFPFIVKNNIDCMAIGQYLFNPHNTPDNFIFLHAGLGIY
                     TSFFTKEKIGASKNPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFK
                     NSQLTVLKSLVKTEKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPAD
                     KIYINSQLLNYQPFTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVA
                     FFIEHSNVLQDIISP"
     gene            13529..13699
                     /locus_tag="VW561_000234"
     CDS             13529..13699
                     /locus_tag="VW561_000234"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942574.1"
                     /translation="MTKQVPTFTKDTAQLYTCKWLLYNKVTKMYDHTVVNHSVREYIT
                     DSISTNTIEKAG"
     gene            14311..14595
                     /locus_tag="VW561_000235"
     CDS             14311..14595
                     /locus_tag="VW561_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942575.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVILLNKPAKLTNVQKLMEKYKHG"
     gene            14588..14836
                     /locus_tag="VW561_000236"
     CDS             14588..14836
                     /locus_tag="VW561_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942576.1"
                     /translation="MDKMKPVFQALNKELIQENLTLTIICVGGYVLEYHGLRATQDVD
                     AFYDQNQKINEIIARVGKQFNLNIHEELWLNNLVAKQI"
     gene            14932..15150
                     /locus_tag="VW561_000237"
     CDS             14932..15150
                     /locus_tag="VW561_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942577.1"
                     /translation="MMSIREQDLQDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
     gene            complement(15517..17841)
                     /gene="pflB"
                     /locus_tag="VW561_000238"
     CDS             complement(15517..17841)
                     /gene="pflB"
                     /locus_tag="VW561_000238"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEU9942578.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            18038..18329
                     /locus_tag="VW561_000239"
                     /pseudo
     CDS             18038..18329
                     /locus_tag="VW561_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567943.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
     gene            18571..19128
                     /locus_tag="VW561_000240"
     CDS             18571..19128
                     /locus_tag="VW561_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HEU9942579.1"
                     /translation="MAIYHLCIKIISIGKGKSAVVASAYRSGEKIKNEYDGIVHDFTR
                     KGGIAHTEILLPQNAPEEFSNRSVLWNSVEKIEKSKNAQLAREIALPKELSREKQINL
                     VREYVKENFVKVGMCADIALHDKNDGNPHAHILLTMRPLNEDKTWGAKSKKEYILDEN
                     GEKVKLKNGNYNYKTRKINTVDWNE"
     gene            19165..19431
                     /locus_tag="VW561_000241"
     CDS             19165..19431
                     /locus_tag="VW561_000241"
                     /inference="COORDINATES: protein motif:HMM:NF015354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HEU9942580.1"
                     /translation="MTNKYLEENSIQEKVDHRSYQRQGIEQIPTIHLGVSATQMEKKG
                     IATDRGNINRDIKHPKQDFKRNFRMNKSLTKLDKGNTQRRKNRN"
     gene            19466..20032
                     /locus_tag="VW561_000242"
     CDS             19466..20032
                     /locus_tag="VW561_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942581.1"
                     /translation="MLSVFENLIRKKADSNNTDLGKYIESYQFLKEKNIISVSELKES
                     ITDLRDKNYKTTRALKDTEKEIDDKTKLIDQAEKYLKHKDTYKAYTKLKKNKQDTFYN
                     EHTAEIILFESANKYLKEHLGESKTLNISKWKSELTTLKKDKKSLYSQILEIREEVEQ
                     AEKVKTCIEQLQEQEKQLSQVKRNELDL"
     gene            20094..20513
                     /locus_tag="VW561_000243"
     CDS             20094..20513
                     /locus_tag="VW561_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU9942582.1"
                     /translation="MNEREKIIRLWFDMWLTQQDLGIDDIFLDDVIYIESWSPKYENC
                     QTVKHWFNEWNTRGKVLAWDIKQFFHKDNQTIVEWHFKNKMNEGKVEEFDGISLIVWT
                     ADNKIKALKEFGCNCNNYNPYKESETPLFRDEKVNWF"
     gene            20666..21727
                     /gene="dinB"
                     /locus_tag="VW561_000244"
     CDS             20666..21727
                     /gene="dinB"
                     /locus_tag="VW561_000244"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEU9942583.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIKDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKAVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGISYNKF
                     LAKMASDYQKPHGLTVILPEQAENFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYNLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILRAEEDIKKEL
                     TILSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSEKERGVRLLGITLTGF"
     gene            complement(21874..22719)
                     /locus_tag="VW561_000245"
     CDS             complement(21874..22719)
                     /locus_tag="VW561_000245"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEU9942584.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLVATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGIAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            22975..23924
                     /locus_tag="VW561_000246"
                     /pseudo
     CDS             22975..23924
                     /locus_tag="VW561_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(24303..26192)
                     /locus_tag="VW561_000247"
     CDS             complement(24303..26192)
                     /locus_tag="VW561_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEU9942585.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIHLDNLPAKRGVELHAYWPRTNFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSSFEKDCLNLAFSGKKEETLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGGEKALQVGMG
                     ALTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDQAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSTAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            26330..27895
                     /locus_tag="VW561_000248"
     CDS             26330..27895
                     /locus_tag="VW561_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU9942586.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            27895..28659
                     /locus_tag="VW561_000249"
     CDS             27895..28659
                     /locus_tag="VW561_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9942587.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IIAQTTVLKRERTEAEEIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLILNENQRAN"
     gene            complement(28859..29074)
                     /locus_tag="VW561_000250"
     CDS             complement(28859..29074)
                     /locus_tag="VW561_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942588.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
     gene            complement(29943..31193)
                     /gene="ilvA"
                     /locus_tag="VW561_000251"
     CDS             complement(29943..31193)
                     /gene="ilvA"
                     /locus_tag="VW561_000251"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEU9942589.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(31243..31599)
                     /locus_tag="VW561_000252"
     CDS             complement(31243..31599)
                     /locus_tag="VW561_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU9942590.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIH
                     MAHKYHESVTEVFGDE"
     gene            complement(31837..32073)
                     /locus_tag="VW561_000253"
     CDS             complement(31837..32073)
                     /locus_tag="VW561_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942591.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMNFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKH"
     gene            complement(32180..33202)
                     /gene="ilvC"
                     /locus_tag="VW561_000254"
     CDS             complement(32180..33202)
                     /gene="ilvC"
                     /locus_tag="VW561_000254"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU9942592.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(33268..33744)
                     /gene="ilvN"
                     /locus_tag="VW561_000255"
     CDS             complement(33268..33744)
                     /gene="ilvN"
                     /locus_tag="VW561_000255"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEU9942593.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(33737..35437)
                     /locus_tag="VW561_000256"
     CDS             complement(33737..35437)
                     /locus_tag="VW561_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEU9942594.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDALPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            complement(35638..37305)
                     /locus_tag="VW561_000257"
     CDS             complement(35638..37305)
                     /locus_tag="VW561_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEU9942595.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(37308..37673)
                     /locus_tag="VW561_000258"
     CDS             complement(37308..37673)
                     /locus_tag="VW561_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9942596.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(37830..38018)
                     /locus_tag="VW561_000259"
     CDS             complement(37830..38018)
                     /locus_tag="VW561_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEU9942597.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(38134..38310)
                     /locus_tag="VW561_000260"
     CDS             complement(38134..38310)
                     /locus_tag="VW561_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEU9942598.1"
                     /translation="MDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     NGMWRAENVTQDLI"
     gene            complement(38421..38618)
                     /locus_tag="VW561_000261"
     CDS             complement(38421..38618)
                     /locus_tag="VW561_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEU9942599.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCIHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNV"
     gene            complement(39046..40590)
                     /locus_tag="VW561_000262"
     CDS             complement(39046..40590)
                     /locus_tag="VW561_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEU9942600.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLETFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMNPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            40803..41105
                     /gene="gatC"
                     /locus_tag="VW561_000263"
     CDS             40803..41105
                     /gene="gatC"
                     /locus_tag="VW561_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU9942601.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVVEEGTDRDRLFKNVPEKDNYYIKVPAILDDGGDA"
     gene            41105..42571
                     /gene="gatA"
                     /locus_tag="VW561_000264"
     CDS             41105..42571
                     /gene="gatA"
                     /locus_tag="VW561_000264"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEU9942602.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            42571..44013
                     /gene="gatB"
                     /locus_tag="VW561_000265"
     CDS             42571..44013
                     /gene="gatB"
                     /locus_tag="VW561_000265"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEU9942603.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYK
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            44137..45180
                     /locus_tag="VW561_000266"
     CDS             44137..45180
                     /locus_tag="VW561_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HEU9942604.1"
                     /translation="MATMKAARWHAAKDVRIQEIEVPEVLPHQVKVAVKFTGICGTDL
                     HEFLDGPIFIPTDEHVYSGQKAPVTLGHEFSGEIIEVGSDVTRVKVGDRVAVEPILAK
                     NNLVGDYNLDPNLNFVGLAADGGFAKYCVLDGDLVHVIPDSLSYEQAALTEPAAVAVY
                     AVRQSSLKAGDTAVVFGLGPIGLLIVEALRAAGASKIYAVELSPERQAKAEELGAIVV
                     RPEEGETAVEAIHRLTNGGVDVSYEVTGVPVVLGQALAAVHKAGECMVVSIWEREANI
                     NPNEFAIQEKTLKGIIAYRHIFPKVLELMEQGYFSAEKLVTKKIKLENIVEEGFIELT
                     QDKSQIKILVEPE"
     gene            complement(45316..45399)
                     /locus_tag="VW561_000267"
                     /pseudo
     CDS             complement(45316..45399)
                     /locus_tag="VW561_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            45558..46118
                     /gene="efp"
                     /locus_tag="VW561_000268"
     CDS             45558..46118
                     /gene="efp"
                     /locus_tag="VW561_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU9942605.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            46140..46529
                     /locus_tag="VW561_000269"
     CDS             46140..46529
                     /locus_tag="VW561_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9942606.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            46522..46944
                     /gene="nusB"
                     /locus_tag="VW561_000270"
     CDS             46522..46944
                     /gene="nusB"
                     /locus_tag="VW561_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU9942607.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREYT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(46979..48174)
                     /locus_tag="VW561_000271"
                     /pseudo
     CDS             complement(46979..48174)
                     /locus_tag="VW561_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT        15158 a        10159 c         8611 g        14246 t
ORIGIN      
        1 tatccacaat ctcaaaacac tgttttgagc aacctgcggc taacttccta gtttgctctt
       61 tgattttcat tgagtattag tcctttttca aacttcctgc acgagtttgg gttcctgcat
      121 aggcaagtaa gagaagagtt cctgcaatag ctacagatac accattggca attcccgcaa
      181 caatcccttg tgcaaatact ttttctgccg cttcttgata aatcacaaca tctccaagtg
      241 gtgccaagac accccaaaca agggcatttg caagtagttg aatgagatta aaaataagaa
      301 tatctttcca gtcaaaaaca ctattgatca cgcgaacgta ctttctaaaa agtcccacaa
      361 ctaaaccaaa gagtccgcta gcgataatcc aagtccacca tagaccataa ccaacaagag
      421 agtccttgat tgcatgacca atcaacccga caagcaaacc gataatcggt ccaaaaataa
      481 tagaaagtag cgcttgtacc gcatactgaa gctggatgct tgtatttgga acaggggttg
      541 gaatgttgat catcccgatg acgacaaaga gggcagcgcc aattccgaca gcaacaactt
      601 gtttaattgt aaatttgatt tccatactat tctcctattt tatccttcta ttttctttat
      661 ttcaatggtc caagatgaac cgacacctac attataggcc ttggcaaagg aaccttggtt
      721 gatagccaaa cctaaacgat agagagagtt gatgtaaagg atgggttgcc caattctcac
      781 atctgcaaat gatttgccat agacaacctg attttgatag accagcatat cagcatgata
      841 gatggtcact tcaaaacgat caccaaattc tggttccagc ttgtaaaatt cttcccgtgt
      901 gatagaggtc caaagcgaac cgaaacgcac atccagaata tcaatggctc ccttcaccag
      961 atgatcttct atgatggtcg ctacgactgg aagctctaca atctggtcca cactgagctc
     1021 tggccctact tcctcaaaag taatgtgacc actggccagt ttagcaccag tataggcata
     1081 gacatcacga ccgtggaagg tataagaatg ctctgtgttt tgacgcctat tggccacctc
     1141 agaaatctca cgaatggcta caatgccaac gtgtttcttg ataaaggaaa gcgtcccatt
     1201 atctggcgtg acaatgtatt gattttttgc agtcttggca actacactct tacgtttcga
     1261 accgacacct ggatcgacaa ccgatacaaa cgtcgttccc tcaggccagt aatccaccgt
     1321 ctgaaagaga cgatagctcc cctcaaaaat attataaggc gtgatatcgt gcgtcaagtg
     1381 atggattttt aaggttggag actcttctaa agccactcca atcatagccg ataccgcacc
     1441 atcaaccaga ccaaagtctg attgtaatac cagtaaatta ttattcattt tatctccttg
     1501 tatatccttt atcataccat atttcagaga aagtcgctaa tagctatttc aattggttag
     1561 ggtatagttt taccttatag caaatttcct actaaaagtt cttgttatta gctagtaggt
     1621 gcattttttc ttgaaaaagg gtatgatagt tacatcatga aaaagtaggt tttattatga
     1681 aaattatcct tgtcggaggg ggaaaagttg gttttgccct ctgtcgctcc ttggttgcag
     1741 aaaagcatga tgttttgctg attgagcaag acgaagctgt tctcaatcat attgtcagtc
     1801 gctttgatat cattggtatc cttggtaatg gggccgattt tgccattctt gagcaagcca
     1861 gcgtccaaga ttgtgatatc tttatcgccc tgactgagca cgatgaagtc aacatgattg
     1921 cagcagttct agccaagaaa atgggagcta aagaaactat cgttcgggtg cggaaccctg
     1981 aatattctaa ctcttatttc aaggaaaaga atattctcgg tttttctctt atcgttaatc
     2041 ctgagctctt ggctgcccgc gctatcgcga atatcattga cttccccaac gccctgtctg
     2101 tcgaacgctt tgctggtgga cgcgttagcc tcatggaatt tgtcgtcaag tccaccagcg
     2161 gtctttgcca aatgcccatt tctgattttc gtaaaaaatt tggtaatgtc attgtctgtg
     2221 cgatagagag ggatcatcaa attatcattc caagcggtga catgactgta caggataaag
     2281 atagaatctt tgtcactggt aaccgtgtcg atatgatact cttccataat tattttaaat
     2341 cacgcgccgt gaagagcctt ctcatcgttg gggcaggtag aattgcctat tatctacttg
     2401 gtattctcaa agatagtcgt atcgatacaa aagtcattga aatcaatcct gaaatcgcca
     2461 gcttctttag cgagaaattc ccaaatctct acatcgttca aggagatgga accgcaaaag
     2521 atatcctgct ggaagaaagt gctcaacact atgatgccgt tgcgactcta acaggagtcg
     2581 atgaggaaaa tctgattaca tctatgttcc ttgacagggt aggtgtacag aaaaatatta
     2641 ctaaggtcaa tcgtaccagt ctcctcgaga ttatcaatgc gcctgatttt tcaagtatca
     2701 tcacacctaa aagcatcgct gtagatacga ttatgcactt tattcgtggt cgagttaatg
     2761 cccagtattc agaccttcaa gctatgcacc atctagccaa tggccaaatc gaaaccctgc
     2821 aattccatat caaggaagcc aataaaatga ctgccaaacc tctttctcaa ctgaaattga
     2881 aaaaaggggt tcttattgca gccattattc gaaagggcaa gactattttc ccaactgggg
     2941 aggatatgtt ggaagttgga gacaagctcc tagtaacaac cttgttgcca aacatcacca
     3001 agatttatga cttgatcgcg aggtaagaaa tgaataaaag tatgattcgt tacctccttt
     3061 caaaattact tttgattgaa gctgttcttc ttttggttcc tgttgcgatt gctgtctatt
     3121 accgtgaatc gagccaagtc tttacagccc tcttttcgac catagggatt ctcgtattgt
     3181 taggcggttc agggagttta cagaagccaa aaaatcaacg gatttatgcc aaggagggag
     3241 tcttgatcgt tgccctctgt tggatccttt ggtctttctt tggtggtctc ccctttgtct
     3301 tttctgggca aatccccagc cttattgatg ccttttttga aattagttct ggatttacaa
     3361 ctactggagc aagtattttg aacgacgttt cggttctcag ccgttccctc ctcttctggc
     3421 gaagttttac ccacttgatt ggagggatgg gagtgcttgt ttttgcactt gctattatgg
     3481 acaatgccaa aaatagccac ctagaggtga tgaaggctga ggttccaggt cctgtttttg
     3541 gcaaggttgt atccaaacta aaaaacactg cccagattct ctatctcctt tatctagctc
     3601 tcttctccct ctttgtcatc atctattatc tagccggtat gcctctctac gatagttttg
     3661 ttattgctat ggggacagcg ggaactggag gctttaccgt ctataacgac ggaattgccc
     3721 actatggcag ctcactgatt acctatctgg tcagtatcgg agttctggtt tttggagtaa
     3781 atttcaacct ctactactac ctcatgctcc gtcgcatcaa ggccttcttt ggagatgaag
     3841 agcttagggc ttacttggtc attgtactgg tttctacagg cttgattagc ctcaacaccc
     3901 tctacctcta cccagggttt tcaaagagct ttgaaatgac cttcttccag gtttccaaca
     3961 tcattacaac aactggtttt ggatatggag atattaccaa ctggcccctc ttctcccagt
     4021 ttatcctcct tttcctcatg gcaatcggtg gctctgctgg atcaactgca ggtggactca
     4081 agattattcg aggcctcatc ctttcaaaaa ttgccaaaaa ccaaattttg tcaatcctat
     4141 cgccccaccg tgttttgacc ctccatgtta ataaaacggt gattgacaaa gatacccagc
     4201 ataagattct caagtacttt gtcatctatg ctatgatttt gctagcgctt atctttattg
     4261 tcagcctaga tagcaatgat tttctggtcg tgaccagcgc tgtctttagc tgtttcaata
     4321 atatcgggcc tattctagga accacttcta gtttctcaat ctttagtccc atctcaaaaa
     4381 ttctcctatc ctttgcaatg attgcagggc gcttggagat ttacccaatc ctccttctct
     4441 ttatgaagag aacttggtct aagagataaa atacaaaatc cctcttttta caattgtgaa
     4501 agagggattc ttatcttaaa aaagggggaa cgtttttaaa tccaagccta tctgtaaaca
     4561 aaatctttac tcaaataaaa gatggaaaaa cttactacac agcttggtaa tgctccaata
     4621 caaattgtaa ggctttactt ggactctttg ttaaatgaat atattccatt cctctggtag
     4681 caactatctg aatacctaat ctttctgcat ccaccttcat ctctctataa taactccgaa
     4741 cttgtggggc cagaataatt aaatcataaa cacccataat atcataatga gctccgtacg
     4801 ctcctgaatt cgcaatcact ctaacctctg ttaagttagc cccctcgtta attgcattgg
     4861 ctaattgcgc acttgttcca gaccctgcac aaagaaccag tactttcaac tccttacttt
     4921 ttatttcatc tatctcacca ggcattattt cacttgtatc ctcctctaaa ataatatcat
     4981 ttgagcttgc aatatcttct ttcaccagta actgtctatc atacgctcta cagaatggca
     5041 aataaatcaa tatgtcgaca actaaaatca aagataaaaa tacaaaggag ataagttgaa
     5101 aattcgttcc aattaacaat cccaagggac caggaaaggt ccaaggtaac tggatataaa
     5161 atccattcat tccaaagaaa tcaataaata cctttcctag aaatacattc actggtggag
     5221 tcatcaaaaa agggacaaaa agatagggat tcaaaataac aggcatacca aatagaagag
     5281 gttcatttac cgcaaataaa acaggagtaa ttgtagcttt acctaccgat tttaattgtt
     5341 tagaccgcat aaagaaaatc aaaataaatg gtacaacaaa ggtagccccc gttcctccaa
     5401 tagcagcgat ataattcccg aaattaggtg ttaaagaatg ataagggaac tgcccatttg
     5461 caagaagttg agcattctct tccatattgc tcagttgcaa agctgtaaca gcaggtaaga
     5521 caatagatgg tccatgaatt ccaacaaacc aaagcaaagc acacataaac caaatcaaca
     5581 tcatagcagg atatgattct gcccccttaa aaataggagt caatagttgt tgaaatactt
     5641 gggcaaaagg aacatctaaa ctaaaccgag atacaatatc taacaaacca ctaataagta
     5701 aaacaaaaga aaaagggaaa gtatctctaa aagcttgtga tatagcccca ggaacttcct
     5761 taggtaaatg aatagtaatg tctcgcttaa tacagaatcg gtaaacattt acagtaataa
     5821 aggcactgac aaacgaagtt atcaatcctt gagatcctaa atagtttgtc gacaaacttg
     5881 tagatcccgt tttctcatca actacaagcg ttacagttaa tacaagatag ccacatatgg
     5941 ccgccaacat tgcagaagta tcatttatta cctttccagg aggcattttt ctattaacat
     6001 ttccagccaa tgactttgca actgtccctg aaacaataac acccaaaact cccatggtta
     6061 gcatatacac tttcctcatc caggcaatag ctggactcgg taactctact ccgaaatttt
     6121 taggaatcat aataataatc atcaaaaagc ttgaaaacat aattaaaggc atagtagcaa
     6181 taaatccatc tttaatagcc atcatataat tattagtaga aattttttca aatttccctt
     6241 gttgcttttc aatggcagcg actaacttat ccataaaaac tcctcctgta aaaaatttaa
     6301 gctcatagtt aaaatatatt aaacatcagt caaatcgcta accttaattc cgttttaatc
     6361 cttttaccca aagagcactt ttcttaatat atctcttctg tgtttcataa tcaacaaaga
     6421 aaagaccata tcgcttattg tagccattcg cccaagaaaa ttgatcttgc aaagaccata
     6481 taaaatagcc ttgaatattg actcccctat ctcgagcctc taaaactttg tgtaaatgtt
     6541 gctcaatata acggattctt ttactgtcat caataatatc attctcgccc tctggcttaa
     6601 cctctttaag ggctgtacca ttttcagtta aataaatgac cggatgttga ggatattctt
     6661 ctttgatacg aagcaacata tcaaataacc cttgaggata aatattccaa tcccaatctg
     6721 ttgtcggaat accaggcttt tttacaaatt cacctagagc atttaattta aaagaagact
     6781 gtccttttat tcctgttgaa ttaaattctt gtctattttc acctttgtat tcacgaataa
     6841 attctgaacg ataataattc atcccaaaca tcgtattaag aggtgcagct cttttcataa
     6901 tttctaaatc accatcttca ataataaagc tagaattatt agcttccaat atttgattca
     6961 agtgaaaaag cgtgtcatca ctgtagtagc ccaaaaaagt tccatctaat agaaatttat
     7021 tattataaac atcataccgt ttagctgcta aaatattttc tttttgccca tcaataggat
     7081 agcctggctt taaagcatga atacaaccta tctttccctc aatccctaat tgatgaaatt
     7141 cgaggactgc aagagcatgc gccaacaaca tattatgatt cgcttgaatt gcttcagata
     7201 attgaaaatg atgatttgga ggaaactgac ctcctatata ttgacctgca gcaagagaca
     7261 tcagttcatt gattgtaaac caatgcttga cttctgtaaa ttcttggaaa caaaagcgag
     7321 catatcgtat gaaacgatca atattctctc tattcaacca atcccctgtt tctaacattt
     7381 tctgaggcga atcaaaatga tgtaaagaaa caaacggaat cacattatgt tttaagcaag
     7441 actgaaagac tctatggtaa taacgaacag ctaatatatt aggctcccca tctatatcag
     7501 gaaatatacg aacccaagaa atagataaac gcaaagcctg caaaccatgt tctgccgcca
     7561 aagctatatc ctcttcgtaa cgataataaa aatcactagc tggatctggt aagaacggac
     7621 tgttttcttg caaataaaca tcccacatat ttgttccttt gccatctacc ctagttgtac
     7681 cctctacttg ataagcagcg gtagcagctc ctaataaaaa ctccttcggt aaggctttaa
     7741 cctgtaaatt ttccatgact aatcctcatt gcaaaccaat atgttcttca atctttcgaa
     7801 ttcgttcata ttcatcaata aaaaacttga cctgatcata caatagcatc gttgtcatca
     7861 aagtatcttg accatgaatc atgataaaac tcacttcaaa attatctcca gatgcctcct
     7921 ccgctaataa attagtctgt tctcgatggg cagacactat tgagtcgttt gcagactcaa
     7981 ccaattctct tgcctgttca aaatatccat ctctcgcaaa cgccaaagca tccatcaaat
     8041 ctgaccttgc atctcctgcg taagcgacaa ttgtaaaacc aagcatattt atttcttctt
     8101 ttgatttcat ctttcttcct cctcaatctt gatccatctt ttacacatca ttattaaaat
     8161 gacttcgcta cgatataaat agaaagtcga ccctaaatat cttaaaaatc cttaaatttc
     8221 ataatattta ggatcaatct tctctgaata ccttggttca ttttctatac ttatgattta
     8281 taagtggaaa ccagcgtaat tttttgctac ttcaattaca cgataaacat ccaaattaga
     8341 ttggaatagc agttcgtgcc cacggatata attcaacaca tttctccatt catcttgagc
     8401 ttgccttgtt tttccggttg cttgaagata taaagccttc gccatcttaa caacatattc
     8461 acgatgataa ctgagctgtt tccattcatc cttttgacta tttaataact tagaaatatt
     8521 ttcctcaata attggcaaaa aattatcagc tagattgtaa gctatataat aatgattcgc
     8581 taaaacatca ttttgacgac tgccaattgc attaaaataa tcacatgagg aataactgga
     8641 taatttttct aaatattcaa caacagactg ccaatttgac ccatacaaag cagaaaagta
     8701 ttcttcaatc aattcttcat aacttcttgt cttcttccag agcatttccc ccataacata
     8761 attaggaaaa ttatgaggga atccggcacg taattcttga cacgaaatgt aaccgttcaa
     8821 atgtaggtta gaaagataag atacatctct gtaaatggtt tgactaattt tcatatagcc
     8881 taaatcgcca taatgagcac gccctaaagg atagtcatat acgaaactat ctcctttaaa
     8941 tgttttttgc cactcaaaaa gataagataa attttcctca agagaattcg gaagtataat
     9001 tttattacgc agataaggtt taggcgtagg tatggaattg tcaaaatcta catctgcata
     9061 actcatttca aatgttcttg taatcggtgc aaacatcatg gtaaagcgtt caggattatc
     9121 taatttttct ttctgaggcg cccatagcaa ctcatggtaa agcagaaaac aaatctttgt
     9181 atctaatccc tcactcgtta aagccctatc caattgattg agaatacgaa tatactgatc
     9241 cgaaaccaat tcttgtctac agttttcgca ttcacaaata ttattacgag catccgacaa
     9301 ccatacatgt aagtagttaa catcaggtct tttcttggca taatccttga taatttctac
     9361 catcttatcc gccacatctg gatttgaaaa atccagactg gttaaaatcg gagccgtatt
     9421 aaataattct cgtttcccgt ttatttcagc gacatacggt ttcttctcct ctgaaatact
     9481 aagacctgat tcccagccaa attttgaaga gtaacctaaa acctcagctg tccatccatg
     9541 accaacacga tgatgaataa gacctctttt ttgcaattct ttatccaacc tatcactcaa
     9601 ttcttgtact aattcatttg agaatttttc tttatttagg tatggattaa actcatgttc
     9661 ataccaacgt ttcaaaaaag agtaaggatt ttcaaactgg atgaaaaaac tgttcatccc
     9721 aatcttaggt agccaatcaa tgaaatctag tatattttca aatgaatccg ctccctctat
     9781 acatactccc ctatgatagt aactggctgt ttcatctata gatagctgtt tatctaaaaa
     9841 atcttcaaat cgtaactctg gaacaaagtc atgtctgcgc cctggtctag tatacacaac
     9901 cccaaattca tgaaacattc ggtaaattcc aataagtaaa cctacaattg tattagactc
     9961 aattctcccc ttacccttgt ctaatgagat aagaacatgg tcttttctgt cctctttatt
    10021 gactaatcct acaaaaatat ccgcttcctt ctcctcgaca agctctatgt tagtcttaaa
    10081 gacccgattc atatagtaac ttaattcctc taaggcaaag ctagaataag tatttttctg
    10141 ttgatcaaat actctgataa ccatcttatt tccctccaac agtagctttt tctttttcaa
    10201 gattgcgttt atctgcaatt ttgaaaaatg gataataaat tgctacagaa accaaaatca
    10261 aaacaacttg taatagagct cctcgccaac tagctcctgt cgcaaggaag cctcctatga
    10321 tcggtggcat tgtccacgga aggattacac ctgttgtata gggtactaat cctacagcca
    10381 ttgatacata ggtaatcaag gcattgattg taggagtagc aataaacgga atcagcataa
    10441 tcggatttaa aactgttgga aacgtaaata gaatagctgt attgatatta aaaatagacg
    10501 gtataatagc tagcttacct aatgttttat tcgcacgact cttactaaat aggaagagac
    10561 aaatcgcaag accaatagtc gctccaccgc caccaataaa tacaaataaa tctttaaacg
    10621 gtaatgtaat aatgtgttgg agtgtttgtc ctgcagccac agcttcttgg ttttctgtag
    10681 taaattgtaa ccaaactggg tctacaaaag catttaaaac ttgtccccca ttaactccac
    10741 agaaccaaaa gaatgagttt acaataacac atagaatcat acctggaagc gttcctgcaa
    10801 ttaacttaag cggtgttcca acaattgcac ctaggagtcc gttgagacct cctgcaactc
    10861 ctgccgcttc aagacctttt aagaccagag cccacaaaac aaaagtagta aagccaggta
    10921 aaagagctga aaatgattta cttaccacat ctgggacacc acttggcatt tttatcgtaa
    10981 ttccgcgctg gataaacata cgataaatct ctgctgtaac caatccaata gtaatcgcca
    11041 taaacaaaga agatgcattc aggctagaaa atggtattgc gccgccaaat aactgcttga
    11101 cctgctcccc atttttatca taaaccatac tcgaaaaacg aggtgccatc aatacaaaac
    11161 tggataaggc tatgatccct gccgacggac catctgtacc atatccttcc gacaacctgt
    11221 aggcaatacc gaaacaagcg accaaggaga taatcgctac tgttgactga ttcatagttg
    11281 tcaagatact tagcaatcca acactattca gccaatttgt aaaagcctct tgaggccaac
    11341 tgattagaat aagaaataaa gagccgacta aaatcaaagg cattgccatc ataaatcctt
    11401 cacgaatagc tttcaaatgc cgttggttgc caactttttc accaaacgga gcgacttttg
    11461 tttctaaaaa cgctaaaata ttgttcatca ctggaactcc tttttttatt ttttataaat
    11521 taaatatacc actaacagaa agcgttgtca atatattttt tgttgttttt acaatatttt
    11581 tttctttgta tagaataata attttaattg attttttctt atgtttaata taaaataaac
    11641 caaaggagga aacaaatgat tacaaaacaa tcagcctttt tacaaaatag agccactata
    11701 ctggagtttc tatatcgaaa tccagcaaca tcccgtacag atattgttaa tgaaacagga
    11761 cttactccag caacaacgac taacatcata aaagagctaa gcgaacagtc cctcatatac
    11821 gaaactggtg atgaatttag cgagttttcc ggatctggaa gacgtagaaa aactatttct
    11881 atcacagata atattccgta tgttgttgga ggtattgaaa taaacgtcct aggtatcttt
    11941 ctcagtctat gtgacttaca agggaaaact cttttcgaga cagaaatttt gaatgaagat
    12001 tatcctattt cagaaatcaa ttccaccatt accaatatga taaaaacagc tatagagtac
    12061 gtccctttgg aaacaaaatt acttggattt ggcttatcaa tacctggaca ttataacaaa
    12121 gactccggaa gtatcattac aaacaacccc atatgggaat cttttaattt attaaatgta
    12181 attaaaagat tcaattttcc ttttattgta aaaaataata tcgattgtat ggctatagga
    12241 caataccttt ttaatccaca caataccccc gataacttta ttttcctaca cgctggatta
    12301 ggtatttaca cttccttttt cacaaaagaa aaaataggag cctctaaaaa tccttatatc
    12361 ggagaaattg gacacaccat tgtcgaattg aatgggcaat attgtgaatg cggaaaaaaa
    12421 ggttgtttac aaacatatat ttcggatgct tggttaatca aacacgccca attattattt
    12481 aaaaattccc aactaactgt actaaaaagc cttgtaaaga ctgaaaaaga cattcattta
    12541 gacacccttt taacggctta taatttaggc gactccgctt tacgtcaaca aattgataaa
    12601 ggagtcaatt tattagccac ttctattgca aatctcctcc tcatcaatcc tgctgataaa
    12661 atctatatca acagtcaatt gcttaattat caacctttca ctcatgaagt cagggataaa
    12721 atccaagacc agctccactt cgttcccttt actcgtaata tagaaattga aattttacct
    12781 tacaacaaac atcgtggaag tataggagct tgtgcattag ctatcgtcgc ttttttcata
    12841 gaacatagca atgtattaca agatattatt tcaccttaat atattagaaa tctatagacc
    12901 tgtttaaatc aactataacc tgtagtagat atctcgtatt tagacaatat gaaaacaaga
    12961 cgacttccat ataggaaacc gccttctctc tatgttgagt gatttatatt aaaataactt
    13021 ttcttctagc tgcattttat tattataaaa acattcatca taaccctgaa cttaaataac
    13081 gatttttatt caagatacat actcctagaa taaactttat atgaaattct catttttgtt
    13141 tttacaattc tccttagtta aatcttgttt aatatatgtt ttacatatag tatttagcgc
    13201 cacatagtac tgaactctct ccaaaaacgg ttattcctct ttgaataggg cgttatcaca
    13261 agaaaagcat ctccacgttt caacttcata tggctcaaaa acaatcaatt gatgctaaaa
    13321 cctgtaccta gatgtttcgg ttcataaaac catgaaactg taaaagtgga tgaaattgat
    13381 agcgatagtc aaatcaagag gcatcataac tctaaaaagt cacaatatat aagttcatcc
    13441 tcggaaaaat atcattctaa ttgttgaaat gcctacatga aaagaaacgt caaatactca
    13501 tgaaacaacg aatacaggta tcaaaactat gacaaaacaa gtccctacat ttactaaaga
    13561 cactgctcaa ctttacacct gtaaatggtt gttgtataat aaagttacaa agatgtacga
    13621 ccacactgtt gtaaatcata gtgttcgcga atatattact gatagcattt ctacaaatac
    13681 aattgaaaag gctggatagg ctttaaacga aatatgtcag tgttttgaca ttcctagcct
    13741 tcatatcatt taaagaattg tatagataaa attttttcca atacagacac tcgtaacaac
    13801 tgcttcattt ttctaccaac atatttagga acaggatacg atacaagagg attaatccat
    13861 agatcagttc tataccaatc taagacaaat aagctaaaaa aacgattgat aataagcaaa
    13921 tagattccaa attttctcta tctgctcatt ttaataaaca atactagtgt aactatcctt
    13981 ccagtcagaa gcttgtcaaa tcacaccgaa aattcttcta aaatttatct cgttaggcaa
    14041 tcaagcaaaa actcgacgat agtacaaaca ttatcataca ggattgactt cctaaattat
    14101 atactttagt aaggttttcg gataagaaaa aaggttcatt ttacatttct aaacattctt
    14161 ttctaagatg aaaaacagaa tttttcgatt gtgatttaaa gcaacaagaa gattttcagt
    14221 atcatcctat agatacgagc taattaagaa aaactacatt tttgaatata aactacaata
    14281 agataaacta aatttcatag gaggaagaca atggattggt acgattatat gatacaggca
    14341 tccaaacaat cacaattcaa cgcaagccat tggtttcgct atttgcgaaa agttattttt
    14401 gaagactatt cttatttaac aaaccaagat gtagaaaagt tgctagactc caaagaacta
    14461 acccgttttc aaaaaattag cttgaagtat gcctttcaag agcatactcc aactcataaa
    14521 tatgtgattt tattaaataa acctgctaag ttaaccaatg ttcaaaaatt gatggagaaa
    14581 tacaaacatg gataaaatga aaccggtctt ccaagcccta aataaggaat taattcagga
    14641 aaatctgact ttaacaatta tctgtgtcgg tggttatgtc ttagaatatc atggtttacg
    14701 tgccacacaa gatgttgatg ctttttatga ccaaaatcag aaaatcaatg aaattattgc
    14761 tcgtgtagga aaacagttta atcttaacat ccacgaagag ttatggctca acaatcttgt
    14821 tgcaaagcaa atatgaacaa acaacctccc ttaagtttat gcgaatcctt gtactcattt
    14881 gagaatctga ccgttttagt agtccctatt gagtatgtgt taggaatgaa gatgatgagt
    14941 atcagagagc aggatttgca agatattggt gcaattatta aatacaaaaa ctttcattca
    15001 ccattcgata cctttaaata tttaaaagac atgggatttg atactattga cctctctgtt
    15061 ctattagaag gttttagtta tgcttatggt atggattggt tagaaaaatt ttttaaagaa
    15121 aatcaagata aacttcgcga attttattaa cagtatcaaa caagatgatt gtcaattagc
    15181 cagtcgtctt atttttatga tggaatgaaa taaaatcgga acaaatcact tagagaattc
    15241 cagtcaattt ctagcactgt ttgagacgtt tcagcgaact atagtaaaat gaaataagaa
    15301 taggacaaat cgatcaggac agtcaaatcg atttctaaga atgttttaga agtaaaggtg
    15361 tactattcta gtttcaatct attatatttc agattcggtc cactattcct tcccaaaaac
    15421 attgcatatt gcgccgaagg aattgctcac actatcccaa gcacaaaacc ctcagtcgat
    15481 tgaccgaggg ttccttttca ttattcaaaa acttgattag ctcaatgcat ccaaggcatc
    15541 atccattgaa agaacttcgt ggaagacacg ttgtgtcaat tcagtttttt gttctggagt
    15601 gaggtattta gtgtttacac agtatccaga gatacgtacg ataacgtctt cgcctgacat
    15661 gatcttttcg taaacatcgt tcaagtccat aacgttcaag ttaacgtgtt gtccaccgtt
    15721 ttcgaagtaa ccatcaagga ttgttaccaa gttatcaact tgttcatcac gagtcttacc
    15781 aagagcgcga ggtgatactt gtgtagtcaa tgagataccg tcagctgcat aactaaagtc
    15841 aaggctagaa agtgagttca agttttgcaa ccaaccacct ttagctttgt tagatgggtt
    15901 agcacctggt gagaagaatt caagtttaga caagttcaca gaaccatctt cgttgaggta
    15961 tacacctttg tgaactggtg agttaccagt ttgtttagag taagcaacgt tagatgtgat
    16021 tgtcaaaagt gatactgtag cttctgcgtc tttgtatagt ttgtggctac gtagacgagt
    16081 tgtgtaagct tcgatcaacc attctgccaa ttcgtttgaa cgtgggtcat cttcacccca
    16141 gcgtgggtag tcaccgattg tttcgtaatc gtagatgtag ccatcttcgt cacggattgg
    16201 tttaactgta gcgtatttga tagctgacaa tgtatcaaca gtgttagcaa atccacagat
    16261 accgaatccc atgttggcac gttgtttagt tggcaagaag gccatttgaa cagcttcgta
    16321 gttgtaccta tcagtcatgt agtggatgat gttcaaggca tctacgtaag tgtcagtcaa
    16381 ccagtcaaga gatttttcaa agttcgcttt aactgattca aattcaagaa cttcgtcacg
    16441 gattggttcg atatcaaata ctttgtagtc tttgtgaaca tcgtcgtaac caccattcaa
    16501 accagtaaga agggctttaa gaacgtttac acgagcaccg aagtactgga tgttgtggcg
    16561 ttgttcttca ttttctggat caagtggaga cacacagcat gagatacagc tcatttcacc
    16621 atatccgtct ttagccattg ttgttacacc ttcgtattgg atagaagagt gtttgtggct
    16681 catgtgcata cagtagcgac ggaagttgta tggcaatttg tcagtccaaa gaactgtcaa
    16741 gtttggttct ggtgagttac cgatgttgtc aagagtgttc aagaaacggt agtccatctt
    16801 agtaacacgg tgacgaccgt cgttacccat accagccata gaagttgtga taaaggttgg
    16861 gtcacctgag tacaattggt cataagcttt ggtacgagca aatttaactg tacgaagttt
    16921 cataacgaaa tcatcaacga attcttggat ttctgattca gtaaatgtac cacgagcaag
    16981 gtcacgttct gcaaagatgt ccaatacgat tggtacacga cctagagatg tagcagcacc
    17041 gttaatcaca cggcagacag ccatgaaagc aatgttaacc cattggattg cttctttcac
    17101 gttcatcgct ggtttgcgaa catcaacccc gtaaaggtca cccaggcgaa caacttgttg
    17161 caatgcttgg tattgaaggt ttacttcttc acgaagacgg attgtttctt catcgatttc
    17221 tttgattgca ttccagtcgt ttactttttc ttgcatcaag tagtctgcac cgtaaagagc
    17281 aagacgtgcg taaacaccga tgatacgtcc gcgtgagtat gcatctggaa gaccagttac
    17341 agtgtgtgca tgacgagcgc gacgaatatt tgaagtgtag gcacggaaaa taccgtcgtt
    17401 aactgttgtt acatatttag tgaagatttc gtgaacagct gggtctggtt cgtatccatt
    17461 ttcttttaaa gtagtttcag ccatacggat accacctttt ggcatgaagt tcaatttgaa
    17521 gagttcatca ttttggatac caaagataac ttcattttct ttgtcgataa atccagcagg
    17581 gatatcagcg atagatgttg gacgagtgtc cattgggaaa cgagtttctt cgtagtgtgc
    17641 tttagtttct tctacaattt tcttgatgtg aagtgaacgc tctgttggtc ctgcaaggaa
    17701 gctttcgtct ccatcataag gtgtgtagtt agcttgtaca aagcgtgaca cacttgcttt
    17761 ttctttccaa tctacgcctt tgaagccttc ccaagctttg tcaaaaatat cttgtgcttc
    17821 aacaactgtc ttaacaacca tattaatgtc ctcttttttc tagtaacagt catctgttac
    17881 attcatgaga caagtatacc atatagtaac cgatttcaac aagtgaaaaa tccctatttt
    17941 tacactttct tttctaaaac agtctatatt ttatttcaaa ctgtattata tttttgaaaa
    18001 aataaagtcc ttttttcttt ttttcagaaa aaagggtata ataaaagaaa ataagcagtt
    18061 acaaaacaaa agaaaaaagc tcattagtca gcagaaagaa gaggaacgaa aaaaaaagag
    18121 atagacggct ttatgaaaaa ggaggagtct ttgaaagtat ctttaccgaa agcaagaact
    18181 taaccaaaga tgaattttat cagctaatta cattccaaaa tatcaaagaa gcagtcaatc
    18241 aaaaaatcct aaaaattatt gagaagcgag aacaatgcga aaatcaaaac acagaaaacc
    18301 aagaggaaga aatagaaaca gaggaatagt cccttgtata caagggcgca cttatacacc
    18361 ctaaagggtg tgtgcgttct ccgaaggctc ttagcagagg gcatatcagc taacgctgat
    18421 acaggggagc tacactcccc tacgaaaata aatttcctac cccttgtata cttcccaaaa
    18481 gaaatcggat aaaaagctat ccgatttctt taagaagtct tattcatcgc cacatcaaag
    18541 tattagaaaa acgaaaggag gttttctctt atggcgatat atcatctttg cataaagatt
    18601 atctcaatag gtaaaggcaa aagtgcagta gtagcttccg cctatcgaag tggtgaaaag
    18661 ataaaaaatg aatatgacgg aatagtccat gactttacaa gaaaaggtgg aatagcacat
    18721 accgaaatcc tattaccaca aaatgcacct gaagaatttt caaaccgttc tgtattatgg
    18781 aacagtgttg agaaaataga aaaaagtaaa aatgcacaac ttgcaagaga aatagcatta
    18841 cctaaagaat taagcagaga aaaacaaata aatcttgtaa gagagtatgt aaaagaaaat
    18901 tttgtgaaag ttggtatgtg tgccgatatt gcattacacg ataaaaatga cggaaaccca
    18961 cacgcacata tcctacttac catgcgaccg ttaaacgaag ataaaacatg gggagcaaag
    19021 tcaaaaaaag aatatatcct tgatgaaaac ggagagaaag taaaacttaa aaatggaaat
    19081 tataattata aaacaaggaa aatcaataca gtggattgga acgaataata caaatcggaa
    19141 gagtggcgaa aagcatggga agatattaca aacaaatatt tggaagaaaa tagcatacag
    19201 gaaaaagtag atcaccgttc ctatcaaaga caaggtatag aacaaatacc gaccattcat
    19261 ttaggcgtat cagccaccca aatggaaaag aaaggcatag ccacagacag aggaaatatt
    19321 aaccgagaca tcaaacatcc aaaacaggat tttaagagaa atttcaggat gaataaaagc
    19381 cttactaaat tggataaggg gaatacgcaa agaagaaaaa acagaaattg ataatctcaa
    19441 atccaccctc ccacccaaag aaaatttgct atccgttttt gaaaatctta tccgtaaaaa
    19501 agcagatagc aataacacag atttaggaaa atacatcgaa agctatcagt tcttaaaaga
    19561 gaaaaatatc atttccgtat ctgaactgaa agagagtatt accgatttac gagacaaaaa
    19621 ttataagacc acaagagctt tgaaagatac cgaaaaggag attgatgata aaaccaaact
    19681 tattgaccaa gccgaaaaat atttgaagca taaagacacc tacaaagctt ataccaagtt
    19741 aaagaaaaac aaacaagata ctttctacaa tgaacacacc gcagagatta ttttatttga
    19801 aagtgcaaat aaatatctga aagagcattt aggtgaaagc aagaccttaa atatatccaa
    19861 atggaaatcg gaacttacca ctttgaagaa agataaaaag agcctatata gtcaaatatt
    19921 agaaatacga gaggaagtag aacaggctga aaaagttaag acctgtatag agcagttaca
    19981 ggaacaagaa aaacaactat cacaggtaaa gagaaatgag ttagacctat aaaactaagt
    20041 gaaaaaatgg tataattgta tataattatt tgattttgga ggtgcttaaa tttatgaatg
    20101 aaagagaaaa aatcattcgt ttatggtttg atatgtggct cacacaacaa gacttaggta
    20161 tagatgatat ttttttagat gatgtaattt atattgagag ctggagtcct aagtatgaaa
    20221 attgtcaaac agtaaagcac tggtttaatg aatggaatac aagaggaaaa gtgcttgctt
    20281 gggatataaa gcaatttttt cataaggata atcaaactat tgtagaatgg cacttcaaaa
    20341 ataaaatgaa tgaggggaaa gttgaagaat ttgacggtat ttctttgatt gtttggacag
    20401 ccgataataa gataaaagca ttgaaagagt ttggctgtaa ttgtaataac tacaatcctt
    20461 acaaagagag tgaaacacca ttgtttagag atgaaaaagt gaattggttt taaggtgatt
    20521 actatggata tagatagatt tttcaaaaca gttttatcac aaaatgaaga agaacttagg
    20581 aattttttca aaaaaaaatg ctgttataaa atggcactgc acaaatgagt tttttacctt
    20641 agaagagtat ataaaggtgc taggcatgtt gatttttcct ttgttaaatg atttgtcaag
    20701 aaaaatcatc catattgaca tggatgcctt ttttgctgcg gtggaaatca aggataatcc
    20761 taaactcaga ggaaaacctg tcattattgg aagcgaccct cggcaaacag gtggacgggg
    20821 agtcgtttct acctgtagct atgaggctcg agcttttggt gtccattctg ccatgagttc
    20881 caaggaagct tatgaacgtt gtccccaggc cgtctttatc tcaggaaatt atgagaaata
    20941 caaagctgtg ggactccaga ttcgagctat ttttaagcgc tatacagatt tgattgaacc
    21001 catgagcatt gacgaagcct atttggatgt gacagaaaat aaactcggta tcaagtcagc
    21061 ggtcaaaatt gctcgcctca ttcaaaaaga tatctggcaa gaactccatc taactgcttc
    21121 tgccggtatt tcctacaaca agttcttagc taaaatggcg agtgattatc aaaaacctca
    21181 tggtttgaca gtgattctac ctgaacaggc tgagaatttt ctcaaacaaa tggatatttc
    21241 caaatttcat ggagtaggaa aaaagacagt agaacgtctt catcaaatgg gcgtttttac
    21301 tggtgctgat ttacttgaag ttcctgaggt gaccctaata gaccgttttg gtagactagg
    21361 ctataatctg tatcgaaagg ctcgtggcat tcacaactct ccagtcaaat ccaatcgcat
    21421 ccgtaaatca atcggcaagg agaaaaccta cgggaagatt ctccgtgctg aggaagatat
    21481 caaaaaagag ctgactattc tatcagaaaa agtcgctctc aatctacatc aacaagaaaa
    21541 agctggaaaa attgtcattt tgaaaatccg ctacgaggac ttttcaactc ttaccaaacg
    21601 aaaaagtctt gctcaaaaaa cacaagatgc tagtcagata agccaaatag ccctgcaact
    21661 ctatgaagaa ttaagcgaga aagaaagagg tgtccgccta ttgggaatta ccctgactgg
    21721 attttaaagc tcaatgaaaa tcaaagaaca aactaggaag ctagccgcag gctgctcaaa
    21781 acactgtttt gaggttgcag ataaagctga cgcggtttga agagattttc taagagtata
    21841 aaaccttgaa gagttgcccc ttcaaggttt ttcttataca aataaacgga caaaactgta
    21901 aagtagcaaa acactaccaa gcacgatacg gtatttacca aaaagggtga agtcgtgttt
    21961 tttcacatag ctggtcaaga agcgaatagc caccatgctg accgcaaaag ctactcccat
    22021 cgcgaccaag agcaaaaaca attgcccaaa gctcaagagt tctcctgctt ttacaaattt
    22081 gaaaatcttt aaggcactag ctccaaacat aacgggaatc ccaagataga aggtaaattc
    22141 tgtcacaact gaacgactgg ttccatttaa caaaccaccg acaatcgttg cacctgaacg
    22201 gctagtccct ggtaaaagag caagaacttg gaagagtccg atatagaaag cggtcgtata
    22261 aggaagcttg tccaactctg ttacacttgg ctcgatagca cgcgctttat tgcgcttttc
    22321 caaatagatg aaggcaatcc cgtagataat caacatgaga gcaactgaaa ccatgttatg
    22381 gaagtgggta tcaaaccaat catcaaattt aaagacacca agtaaaggca aagtggcaac
    22441 caagaccttc aaccacagtc tccaagtctt acgaacttcc tgtttgtcct tagtcggttt
    22501 aaaaggattg agcttgttaa aataaatcac cataactgct aaaatagcac caagctgaat
    22561 cacgacatta aacatggaca taaaggcttc attttgattt tggtattgga taaactcctc
    22621 tgctaaaatc aaatgacctg tactggaaat cggcaaccat tccgtaattc cttcaacaat
    22681 cccgaagaag atagatttta aaatttcaat aagatacata gattactccc tatattatat
    22741 ttagcgcagt tagctactgc caaagcccaa gtggtatact tggaataagc cactgtggat
    22801 tagtttattc cctccaatcg cttgacgatt tccgaaaggc tctaaccaca gttttttgtt
    22861 tcaattggta atgagtttga taacgcatag acgttttatc tcgtctaagg taacaaggca
    22921 aaaaattcgt ggataaccaa gtagacttga aatactacat ctggaggtac tcccatgctt
    22981 tatgtgggca ttgatgtcac taaaaataaa cacgatgtta cagccttgaa tgttccagga
    23041 aaaactgttc ttaaaccact cactttttca aataataaag ctggttttga actcttagat
    23101 ctgtctcttc gacaactcaa ccaagactgt ctcatcgctc ttgaaaatac cggacattat
    23161 gcctttaatc tactgaactt cctgcatgaa caaggatata aagtttacac ctgcaatcct
    23221 ttgctcatca aggaatttgc caaatcatta tcacttcgca agactaaaac ggataagaaa
    23281 gacgctcatg gcatcgctct taaacttctt tctgacccca atcgtgaaca atttcaacac
    23341 gataatcggc aagtagaact aaaaatactg gctagacata ttcatcgtct caagaaaaac
    23401 agtctgattg gaaagtacaa tacactcgtt gtcttgatat catctttcct gagttggata
    23461 aaatcgttgg aaagcattca gaatatacct accaactctt gacacgctac cctaatcctc
    23521 agaaaaggat tgaggcagga tttgataagc tgatagaaat taagcgattg accgcttcta
    23581 aaattcagga tatcctctca gtcgcacctc gttctatcgg aacaacatct cctgctcgtg
    23641 aattcgaaat catcgaaaac atcaaacatt acaagaggct cattgacaag gcggaaaaat
    23701 gtgtcaatga cttgatgtct gagttcaact cggtcatcac gacggttact gggattggga
    23761 atcgtttagg ggcggtcatt ttagccgaga ttcgaaatat tcatgccttt gataatcctg
    23821 ctcaattaca agctttcgct ggactggatt cttctattta tcagtcaggt cagattgatt
    23881 tagcaggaag aatggtcaaa cggggttccc ctcatctgcg gtaggtactc atacaagctg
    23941 ccaaagcatg ccctcgcttt tcacctgctt ttaaggccta tcttaagact aagttagaac
    24001 aaggaaaaca ttacaatgta gccatcatcc accttgcaaa aaaacttatc cgaaccctgt
    24061 tttatatcct aaaaaagagc tgccatttga cgaacaaaaa gtgatgtaat ttctgaaatt
    24121 aacacaggat tacctttgaa aaaatggact ttttcaaggg ctttttgccg tgctctaaaa
    24181 tcatcgccaa atcatcaaat tgaaataaga aatagtttag ataaaactat tgacttttct
    24241 tatagtttgt cttttttcta tcttccatta tatcatactt tttcagtcta tggtagctct
    24301 ctttaaaagg caccgatact gccgccacct ccgcctccag agaagccacc accagaactt
    24361 ccacttccag aagatacaga gtaggtgctt gctgtatttg cgacactagc ataatggctc
    24421 atttgtgctg ttgaatgata aaacatactg tgccagccat aagctacata gagattgata
    24481 tctggatttt ccacttgaat ctgatgaacc ttcatcaaat gactaacctt gtccgcatag
    24541 ccaaataagg tcgcatagac caagaggcga ttccagacca caatactttc cagttcagcc
    24601 tgatccaatc gtgcaatctc acgcaacata ttttcaaaac tggtccagag atagtagacc
    24661 tcagctcccg cttcatttag aacaccatca cgattatcta gtcgaagctt ccaataatag
    24721 aaaacagaca aaactaaccc tagaaaacca agtattggca aagggaggta aagatagcca
    24781 tgaacgtcta aactgtacaa gaacaaacca aatccgataa ataggggcag gatagtcaag
    24841 gcacccatac ccacttgcaa ggccttttcc ccaccagtta aaggacgata ataatctggg
    24901 agcccccaga aggaaactcg ttttctcact ccttcttgca tctggttcaa tacctcttca
    24961 aaagaagatt tgagttgaag ccctcttgct tgaatccgtt tttcatcaga aactttggct
    25021 ctacgataaa gactatcaga taccttgtaa tccgcaaaca aattggaaag agtttcttct
    25081 tttttacctg aaaaagctag atttaggcag tctttctcaa agcttgacaa accatcttct
    25141 tttactagcc tcaaaccaac tgcatctcct tctgaaatga tagagacatt cccacggtct
    25201 atcacatcta gcaaggtagc ttgaataagt tgatcaaagg tgaattttcc agctcccttg
    25261 accaagggac tcacttcctc caaggaggtc gagtagactg cttctgataa aaccataggc
    25321 tctaattcca ttggtggttc atagagacga tgatttttgg catatttgac tgaaggagtg
    25381 gtctttcttc tataaataaa atagaagcag acactcaata acaaggagat agaaagtatc
    25441 gaagggagga cccaagtaac gagttgttta ctctgatctt tttctctaac aatcgagtct
    25501 tctatcttat taaactcttc taaacgattc cctttcaatc cctgatccct agcgctagca
    25561 aaattggtcc gaggccaata ggcatgcaac tcaactccac gcttagccgg aagattgtct
    25621 aaatggatag tataatcaag gttactcttt tcaatcgttc cctctctaaa aagtttccct
    25681 gtatggaaaa agagtttttc agcccccttg tctcccctta catgaaattc aaacttttca
    25741 atagactctg aactatctgt cagaggttgc caatttaatt cagcgatatc atcataaagg
    25801 aaaagtaaat tttttaagtt ccagacgagg tcaacttcaa ctatgtcgcc ctcctgacct
    25861 ggattataga ctctcacagt ataaccatcc gcttcttctg ttacttcgct agtcacatct
    25921 gctagttctg caccgttttt cgcggcctga acctttggat gagggtcaat gtcaaacccg
    25981 ctaggcatct taccagcacg tccaagtccc acgatttggc ccttaaagtc ctcctcaaac
    26041 tggtaaacta tcttctgtct aaactctgcc gtattatctg catgaatata caaatcccct
    26101 tgataagagt ttatcttgaa atcaatggca aaaacagaga gtggaagaag gcaaaacaag
    26161 cctaacacca gtaagaaaaa agtttttttc atcaactaag cccccttttt atctttctta
    26221 tcattatagc attttttcgt aagtaaaggc ttacttatgt tgaaaaatta cgcatttttc
    26281 gataaaatag gagacagtta ttttttaata gatcagaata ggagaaatca tgagaaaaat
    26341 atacttatct attttcacaa gtctcttgct gatgctagga cttgtcaatg ttgctcaagc
    26401 cgatgaatat ttacgcatcg ggatggaagc agcatatgct ccctttaact ggacccagga
    26461 tgatgatagc aacggagctg tcaaaatcga tgggaccaat cagtatgcca acggatacga
    26521 tgttcaaatc gccaagaaaa tcgctaagga cttaggtaaa gaacctttgg ttgttaaaac
    26581 caagtgggaa ggtctagtcc ctgcccttac ttctggtaag attgacatga ttatcgcagg
    26641 tatgagtcca actgcagaac gcaaacaaga aattgccttt tcgagcagtt actacactag
    26701 cgaaccagtt ttgcttgtca aaaaagattc tgcctacgca agtgctaaat ctttggatga
    26761 ctttaacggt gcaaaaatca cttctcaaca aggggtctac ctctacgact tgattgcaca
    26821 aatcccaggt gctaaaaaag aaacagccat gggagacttc gctcaaatgc gacaagctct
    26881 tgaggctggt gtcattgatg cttatgtttc cgaacgtcca gaagcactga ctgctgaagc
    26941 tgcgaactct aagttcaaga tgattcaagt agaacctggt ttcaaaactg gggaagaaga
    27001 tacagctatc gctatcgggc ttcgtaaaaa tgacaatcgt attagccaaa tcaatgccag
    27061 cattgaaacc atttcaaagg acgaccaagt tgccctgatg gatcgtatga tcaaggaaca
    27121 acctgccgaa gctacaacaa ctgaagagac tagcagtagt ttctttagcc aagtcgctaa
    27181 aattctttct gaaaactggc aacaactctt gcgtggtgct ggtatcactc ttttaatctc
    27241 tatcgtcgga accatcatag gtctcattat tggacttgcc attggtgtct tccgtactgc
    27301 tcctctctct gaaaacaaag ccatttatgg ccttcaaaaa ctagtcggct gggttctcaa
    27361 tgtctacatt gaaattttcc gtggtacgcc aatgattgtt caatcgatgg ttatctacta
    27421 tggaactgcc caagctttcg ggatcaacct tgaccgtaca ctggctgcta tcttcatcgt
    27481 ttcaatcaat accggtgcct acatgactga aatcgtccgt ggtggtatcc tagcagttga
    27541 caagggacaa tttgaagctg cgactgctct tggtatgacc cataaccaga ccatgcgtaa
    27601 gattgtccta cctcaggtag tccgtaacat cctacctgca actggtaatg aatttgtcat
    27661 caatatcaaa gatacatctg tattgaacgt tatctctgtt gtcgaacttt atttctcagg
    27721 aaataccgtg gcaacacaaa cctatcaata cttccagaca tttacaatca tcgccgtgat
    27781 ttactttgtc ctcaccttca ccgtaacacg tatcctacgc tttatcgagc gcagaatgga
    27841 catggatacc tatactacag gtgctaacca aatgcaaacg gaggatttga aataatgaca
    27901 caagcaatcc ttgaaattaa acacctcaaa aaatcctatg gacaaaacga agtgctaaaa
    27961 gacatttctc tcactgtcca caagggagaa gttatctcta tcatcggaag ctctggaagt
    28021 gggaaatcga ccttcctacg ctccattaac ctacttgaaa cgccaactga tggacaaatc
    28081 ctttatcatg gacaaaacgt cctggaaaaa ggctatgacc tcacgcaata ccgtgaaaag
    28141 ttaggaatgg ttttccaatc ctttaacctc tttgaaaatc tcaacgttct tgaaaacaca
    28201 atcatcgctc agacaactgt ccttaaacgc gaacgcacag aagctgaaga aattgccaaa
    28261 gagaacctgg aaaaggtcgg catgggagaa cgctactggc aagcgaaacc caaacaactc
    28321 tcaggtggtc aaaaacaacg tgtggccatc gctcgtgccc tttcaatgaa tcctgacgcc
    28381 attctctttg atgaaccaac atcagctctc gatccagaaa tggttggaga ggtcctcaaa
    28441 atcatgcagg acctggctca ggaaggcttg accatgattg tcgtaaccca tgaaatggaa
    28501 tttgcccgtg atgtctctca ccgtgttatc tttatggata agggtgtgat tgctgaagaa
    28561 ggtaaaccag aagacctctt cactaatcct aaagaagacc gtacaaaaga attccttcaa
    28621 cgttatctca tactcaatga aaatcaaaga gcaaactagg aagctagctg caggttgctc
    28681 aaaacactgt tttgaggttg cagatggaag ctgacgtggt ttgaagagat tttcgaagag
    28741 tatcaaataa aaagaaaagg ctgcatcaac cgtgcagtct ttttgctgtc ctcaaaatga
    28801 aaaggcagat tctctatctc tactacttga aggtcaagaa tccgctgtta tccaaagatt
    28861 aatcttcaaa tttttcataa tttttttcat agaaatcaat taggcctagg gctgtttcaa
    28921 atgaaatatt ttttactttt gcacgaccct gcgccagagc aatgatagac atttcacgcg
    28981 cattcgtttc tttagagata cggtagcctg tgattttctt atcacgaacc cagccaacaa
    29041 ctgattctac tttttcaaag tttgacttag ccatgttttt ctcctatttt cttctttata
    29101 atatttaatt gtatacgttt ttactacttt tgtcaataaa aaaaacatat attcaacgaa
    29161 aataattagt tcaaaatttt cttacttcct ttttaaatcc gataatatat aggttttgct
    29221 agcctctaca ccataaatat ttgctaataa aaatactgta ttattaccct cttaaggtgc
    29281 gaaattattc aagttcttaa aaaatgcttc tctatttccg taagatagct tatttcttcg
    29341 atttaagaac tgaagatgat agttctaacc tttagaaatc caagccttca ctagctcaaa
    29401 aactcctatc tttcttcctc aataaatcat ctgagagata ggtattcctt atatccttca
    29461 aattcatcac tcctttggat attgaggtgc tagcctagaa gcagactccc gattatctaa
    29521 accatcctta ataaagatga caagacagat aagctcagtt aattacttat atatcttttt
    29581 aatatggtat atttgtcaac aaaaaaatca aatcatacct tatactcaat gaaaatcaaa
    29641 gagcaaacta ggaagctagc cgcaagttgc tcaaaacact gttttgaggt tgcagataga
    29701 gctaacgtgg tttgaagaga ttttcgaaga gtattaaaga cagtgtgact atgcttgtca
    29761 atagatagga aatcaaaatg aaggatggaa atctgagcca aacatcataa gtgctataat
    29821 gaacagaagt aagaattgca cgataagttt cctaaaacca taggaatcgt cccctttctt
    29881 catcttcatt atagcacata tacatcagtt gtacaaataa tattatattt ttttattagt
    29941 cctcagacaa gcatattata aagcgtttca ttaccattta agttaatata agctggatca
    30001 aaaccttcca ttctacggat caaacctgca taatcatgct tatctgctaa agcgatccca
    30061 attaatactg ggcctgttcc cttgctagct cgtttgatat actcaaaacg tgtgatatca
    30121 tcatttggcc ccaggatatc atttacaaac tcacgcaaag ctcctggacg ttgtgggaaa
    30181 ttgaccacaa agtaatgttt gataccatca taaatcaagg cacgctcttc catttctggc
    30241 atacggttga tatcattatt tcctccagaa atgatacaac aaatggtttt ccccttaata
    30301 tattcagcta aaacctctaa agaggcgata ctagccgctc cagcaggttc tgcgactatc
    30361 ccttgcttag agtaaaggtc aatcaaggtt tcagaaatca atccctcatc gacacctacc
    30421 aaagtttgaa tatgttgacg agttgcttca taggtcaact gacctacctt ttgcacagca
    30481 atcccatccg caaatttatc aatttccttg agtttgactg gacctccagc ctcaaaggca
    30541 gctttcatgg aacgcgctcc attcgcctct actccgataa cctcaatctc tggacttgtt
    30601 tccttgatat aggtagaaac cccggcaatg agaccgccac caccaacagg aaccaagaca
    30661 gcatcaaaat caatcgattc ttttcgagct tcttctaaaa tctcataagc aactgttcct
    30721 tgacctgctt gaacatgagc atcatcaaaa ggatcaataa aggtacgatt ttcagagact
    30781 gtaaattctt gagctgcttt ggctgaggca tcaaaggtat ctccaactag tttaatagtt
    30841 acaaaatccc caccaaaaaa gcgaacctga ccaatctttt gttgtggcgt agtaataggc
    30901 ataaagatag tagcaggaat tttcatttca ttacaagtat aggctactcc ctgcgcatga
    30961 tttcccgcag aagcgcagac taccccacgt tcacgttctt ccttgctgag ctgggaaatg
    31021 gcataatagg caccacgaat tttaaaggag cgaacacgct gggcattttc ttttttcaaa
    31081 taaatcttag caccatactt ctccgataaa taatgatcgt aatccagtgg agtattcaca
    31141 accacaccgt tcaagacctt gtgagccttg attatatctt ttgaacttaa catataaaac
    31201 ctctttttct ataaaaaaca gtcactaatg actgtttttt agttattcat ctccaaaaac
    31261 ttcagtaaca ctttcatggt atttatgagc catatgaatc gaaaactctt taggaagttc
    31321 ataacgattt gtggaaatcc aatcatataa ccattctacc aaactttctt cactatcatt
    31381 acgatggaag ataaaatagt ttaaaatttc tttatattct acatcattca aatccaacgt
    31441 gtctacaaat tgactacgag gaacaaaatt agaatctaat tttcttagtt gatctgtcaa
    31501 ttctcccaat tgtaaatcca actgaaaaaa tagtttttgc agttgcctat cagtttcatc
    31561 acatcctgtc ttgatagaaa acaaattggt aatattcata tgatttcaac ccctaaataa
    31621 gtacatctaa tatcccacga atagctgaag ctaaagtttt cgcagtccag tcatttaaac
    31681 ccgattcttt caatccagat gcaataattt gctctatttg atataaaccg ccatctatcg
    31741 tctcatttat tctgcgaaga ggaccttcaa tataggataa agcttctccg aatctaacag
    31801 ctgatttagg tcctaatgat ttaccaacta tatttgttaa tgttttttat ttctcaaaat
    31861 ccatttcgct atataacgag cagatttagt cgcaccagct ctctcaaacg gagaatgaat
    31921 ttcaagacct tcttctctag ctatttgtgc caatttgaag aattgttcaa agttcatatg
    31981 caacctacca tcttcaaaat tagcatcatc ataaatgtta attacagcat tttcatcata
    32041 cgaccaatct gctttatttt ggtctgctag cactattacc gacggtgtaa ttacattata
    32101 caactagaac caataataac gatgcaattt ttctcataat ctttttcata aaatcatctc
    32161 cagcgctata tatttctaat tagttataga ttttgaatgc atcatcgtcg tttttaccaa
    32221 cgaatggcat tgctttacgc aattctgcac caactttttc aatttcaagg ttagctgctt
    32281 gttcacggta agcagtcaat tttggacgtc cagctttata gtcatttaca aagtcatttg
    32341 caaatttacc attttggatg tctgccaaga cagccttcat attttcttta acttgttcag
    32401 tgattacacg tggacctgat acatagtcac cgtattcagc agtgtttgaa atagattgac
    32461 gcattttctt gaatccacct tcgtagatca agtcaacgat caatttcatt tcgtgaagaa
    32521 cttcaaagta agccaattct ggagcgtaac ctgcttctgt caagacttcg aaacctgctt
    32581 cgataagggc agtcaaacca ccacaaagta cagcttgttc accaaacaaa tcttcttcag
    32641 tttcttcttt gtaagttgtt tcaagaagac ctacacgagc cgctccaaca cctttacacc
    32701 agtccatagc aatgttttta gcatttcctg ttgcatcttg gtatactgca taaagagctg
    32761 gaacaccaaa tccttcttcg taagtacgac gtaccaagtg tcctggtcct ttaggagcac
    32821 acatgaagac atctacatcc gcaggaactt tgataaattc aaagtggatg ttgaaaccat
    32881 gggcaaatcc aactgcgttt ccagcttcca agtttggagc gatttctgct tcgtacaatt
    32941 cttgttgaat ttcgtctggc gccaagatca tgataacatc agccaactta gtagcttctg
    33001 ctactgtgta agtatcaaat ccatcttctt ttgctttatc aaaagattta cctggacgta
    33061 caccgataat aacgtcacga cctgaatcac gcaagttttg agcatgcgca tgcccttgtg
    33121 aaccataacc gataacggcg atttttttac cgtcaagtgc tgctacttta acatcttttt
    33181 catattccat ttgaactgtc atagcttttc tctcttttct attatttatt gccttttaag
    33241 ctggtttaac aaatttaagt tggattttta atcgcgggta aatccagttg cacccgttcg
    33301 agcaatattg cgaataccgt atgggcgaat gactcgcaat agggcttcgc tcttttctgc
    33361 atttcccgtc atctgaatgg taatcgagct tggcgctacg tctaccactg ttgcacggaa
    33421 aggttgaata atcgctaaaa tctcagctct cttctcagct ggcgctgaca tcttaaccaa
    33481 aatcacctcg cgctccaaat gaggcttgtc tgtaatatct cgaatgcgaa tcacatcaat
    33541 ctgacgattg agctgtttga tgatttgctc cacttcatca tgagaagcaa catcaataat
    33601 aatagtgata cgcgatacat tcggatcttc tgttgctcca acagagatgc tttcaatatt
    33661 aacctgacga cgagatagga cacctgtaaa gcgattgagg actcctgaac gattttgtag
    33721 ttttgctgtt aacattctac gcatggaact gcacccccaa catctcatga ttactcttac
    33781 cagccggtac cattggtaac acctgttcct tacgagaaat atctacctca attagcatag
    33841 gaacatcctc agtgatgact tcaaggtctt gagccaaggt ctcaggattg tcaaacttat
    33901 agtttttaat accataagcc tgcgccatca attggaaatc aggaagggca tcaaagaccg
    33961 actctgatgt tctgccttca tagaaggatt cctgccactg gcgaaccatt ccaagtgaat
    34021 gattgttcag cataaccacc ttgattggca ccttgtaaat attcaaaata gccaactcct
    34081 ggttggtcat ttggaaacca ccatccccaa caaacaagac tacttcctta tctgggttag
    34141 caattttagc accgattgct gctggaattc caaagcccat tgttcccaaa ccacctgaag
    34201 tcactaactg acgctcattt tggtagggat aatactgagc tgtccacatt tggtgttgac
    34261 caacgtctgt taccacaatg gcatctccat tcgtcaattc accaattcgt tcaataactg
    34321 cttgcggttg aaccacacgc tctttcttgt cataagaacg aacacgattc ttgtctttag
    34381 tgactttctc aatccacttt tcagtattgt tgtgaactgt tggttctgct agcaacattt
    34441 gcaaggcctt cttagcatct ccaactacag gaatgtctgc actgataatc ttgccaatct
    34501 cagctgggtc aatatcaatg tgggcaacct tagcattctt agcgaaagtc ttaggattcc
    34561 ccgtcaaacg gtcatcgaaa cgagaaccaa tactaatcat aaagtccgct tccgtcatgg
    34621 caatatttgc tgcgaatgac ccgtgcatgc ctcccattcc aagaaagagt gggtgactcg
    34681 ttgcaatcgt tccttgtccc aaaagactgg ttaccactgg aatttgatag cgttctgcaa
    34741 attcatttag ttccgtagca gcctcagcat aactaattcc accaccagct aacaagactg
    34801 gctttttagc cttggacaat tgcttcaaga ttttcttgat ttgcatatca ttcggctcaa
    34861 gagtcggctg ataacttggt aaattcactt ctggtgaata aatgaagtct gtttctaaag
    34921 cagatatgtc ttttggtagg tcaattacaa ctggccctgg acggcctgta gttgcgatat
    34981 ggacagcttc cgtaatgata cgcggaatat cagctgtctc acgaacttgg taattgtact
    35041 tagtgattgg catggtaatt cccacgatgt ctgcctcctg aaaggcatcc ttcccaatcc
    35101 ctgctcgcgc cacctgacct gtaaagacca aaaggggaac gctatcgctc atggcatccg
    35161 caatccctgt aatggcattt gttgctcctg gtccactagt aacgacggca acacccaact
    35221 ttccagttga tttggcataa ccttcagctt catgcaaaca accttgctca tggcgcccta
    35281 gaatgtggcg aatgccttta aaattatata tcgcatcata aaaaggcaaa accgcaccac
    35341 caggataacc aaagatggta tcaactccta aatcacgaag tgtttccaaa actaggtccg
    35401 accccgtctt aggagattct aaactgattt tctccattgt tcccctttct ttcctcttaa
    35461 aaataacttg ttactatcat accatttttt caaaattttt caagacaaaa gaagaaattt
    35521 tctgaatttt ctattttaac gtttatttat gaatgttatt tttgttttta ttaaaaagat
    35581 accgatttca tttactaaaa aagaaaaaag aactgatttc tcagtccttc attaatctta
    35641 ttccacacta aataggtatg ggtaaacagg ttgttgacct tggtgaatct cgacttcaac
    35701 gtcttcgaat tcttctacga tttcttgagc gatttcattg gcaagttctt cgcttccgtc
    35761 ttcaccgaca tagaaggtta cgatttcact gtcttcatcc aacatatgtt tcaaggtttc
    35821 agtcaatgtt tggtgcatat cagggtttga cacaagaatt tttccatcca ccatacctag
    35881 attatcgttt tcatggattt ctaagccatc gatcgttgta tcacgcacgg ctgttgtgac
    35941 gcttccgcta acgacatcgc taagagcagc tgtcatacgc tcttggtttt cttcaatgga
    36001 cttgcttgga tcaaaggcaa gaagacttgt cataccttga ggaagagtgc gagcctctac
    36061 cactactgct ggttgctcca aaacttctgc cgcagattga gctgccatga agatgttctt
    36121 gttgtttggc aagaagatga tgttacgggc attgacctgt tcaacagcct tgataaagtc
    36181 ttctgttgaa gggttcatgg tttgaccgcc ttcgataaca taatccacgc cttgagaaca
    36241 gaagatatct gctagacctt taccagctac cacagcaatc aaagcatact ctttttcttc
    36301 agccgacttg ataacttgag tagcttcttt ctcaacctgt gcttcgtgtt ggttacgcat
    36361 attgtcaact tttaccttga ccaagctacc atatttgaga ccttcttgca taacaagtcc
    36421 tggatcttct gtatgaacat ggactttgac aatttcatca tcgttaacaa cgaggagaga
    36481 atccccaagc tcatccaagt agttacggaa ttcatcgtag tcaaaatctt tagcataggt
    36541 tggaccttgc ttaagagcta ccatgatttc agtacagtaa ccaaacgtga tgtcctcagt
    36601 cgctacgtga ccagctacag acttatgatg ctctacattg atcatctcac tcatgttggc
    36661 aggagtcgct acaaagtcct cagatgcaat atattcgcca gtaagggctg aaaggaaacc
    36721 ttcgtagatg aagaccaatc cttgaccacc tgagtccaca acgccaactt ctttcaatac
    36781 tggaagcatg tctggtgttt tagctagagc tgttttagca ccttccaagg ctgcgcgcat
    36841 gacttcaaca gtatcatctg tttgctcagc ttttttctta gcaccgatag cagctccacg
    36901 agaaactgtc aaaatcgttc cttcaacagg tttcatcact gccttatagg caacttccac
    36961 acctgattgg aaggccaggg ccaagtcttg acctgttaac tcgtctttat ccttgatagc
    37021 ttgggaaaat ccacggaaaa gctgagacgt aatcactcct gagttcccac gcgcacccat
    37081 caaaagccct ttggcaagaa tgctcgctac ttctccaact gtagaagctg gcttgtctgc
    37141 aacttcttta gcaccatttt caatggtcat tcccatattt gtcccagtat ctccatctgg
    37201 aactggaaag acgtttaatg aattgacata ttcagcttgc ttattcaagc gagttgatgc
    37261 agcctgaacc atttcttgaa ataagctagt agtaattttt gacacggtta ttctcctaca
    37321 actttgatat tttgaatgta gacatttaca gtctgagcag taattccaag ctggttttcc
    37381 aagctaaagg caacacgctc ttgaatgttt tttgacactt cactaatctt tgttccgtag
    37441 cttaacacgg tatatacatc aactgcaata ctgccatctt cggctgcctt tacgacgaca
    37501 cctttagaat aattttcctt acctagcagg gcttggaaat tatctttgag ggcattttta
    37561 ctagccatac cgaccacacc aaaaatctca gttgctgctc cacctacgac ggttgcaatc
    37621 acttcatctg ttagttcgat ttgaccatct tttgtattaa tttttacagt catccttttt
    37681 acctcaacta gttgatactc tattttatca tatttcagcc caagtgtaaa agcagaatga
    37741 tgtatcagcg gatatttact ctatttttca aacgatgtta tacccacaat aaaagaaaaa
    37801 agaccctaag gtctcctttg cttttattat taaacgcgtt caactttacc tgatttcaaa
    37861 gcacgagctg aagcccaaac ttttttaggt ttaccatcga taagaacagt aactttttga
    37921 aggtttggtt ttacggcacg ttttgtttgg ttcatcgcgt gtgaacggtt gtttcctgat
    37981 acagtcttac gacctgtaaa gtaacatact ttagccattg tgttttcctc ctattagatc
    38041 taatatagcg gatgtgctag caccacatac cgtactatgt tatcacactt tcttgttttt
    38101 tgcaagggaa ttggaagatt ttttatttgt gtcttaaatc aggtcttgcg tgacattttc
    38161 tgctctccac atgccattgt tgattaacag aacaccagaa ttaaaattat gtgtataaaa
    38221 atcatctcta actgcagcta agggtatagc cgtcaagtcc aaatcccaca gctcatctat
    38281 cgattttctt acaacaatat ctgaatccaa atacagtaca cgagactcgc ttacatactt
    38341 tggaataaaa tacctaaaaa agccgcatat gaaagtccct caaaggggag acgataacct
    38401 ttcagaatac tactgtcaat ctaaacattc acaatctcac tattcaaagt ctctaatctt
    38461 ttttccatca attggaacca ttctcgcgga aggtcatcat taaaaacata aaacttaaga
    38521 ttataatgat gaatacaaag agattttatt gttgtttcaa ctttatccat ataagcatta
    38581 tctgcaccta agacaatcgc ttttttctct tctttcactc tttatcttat ttctttttat
    38641 tcccatcata tgtttctacg taaccattat tttcgcctat tcgttcgtaa aaccatacca
    38701 gtggagattt tagatgaagt cccattacgg tttacaattt ttacattacg acacggagtt
    38761 ttacaaatcg atttcatttg ccaaacgtag ttagtaaggc atttagctag gctacctact
    38821 ttctgctacg acggaaaaat ccacaaatca ataccattcg tggatttttc ctagtgaagc
    38881 ggttaggtaa gccgccatag cggttaccga ttattagatt acgacacgga gtttggcaaa
    38941 tcgattctat ttgccaaacg tagttagtga ggcagttagc tagttcgcca aatagcgact
    39001 agcgtccaac aatttggaac tttagttcca attgttggta ctgagtcaca tcttctcctc
    39061 taactctacg tctggatact tgtccgcaaa ccagcggagg gcaaagtcat tttcaaagag
    39121 aaagactggt tggtcaaaac gatctttggc taagatattg cgacttgacg acatccgttc
    39181 atccaagtcc tcaggcttga tccaacgaac ggtcttttta cccattgggt tcataactac
    39241 ttccgcattg tactcgcctt ccatgcggtg tttaaagact tcaaactgga gttgacctac
    39301 agcgcctagc atgtactcac ctgtttggta attcttataa agctgaacgg ctccttcttg
    39361 caccaattgc tcaatcccct tgtggaaaga tttttgcttc ataacattct tagcagaaac
    39421 tttcatgaaa atttcaggag taaaagttgg cagtggttca aattcaaact tgtttttacc
    39481 aactgtcaag gtatccccaa cttgataagt acctgtatcg taaaccccga taatatcacc
    39541 tgccacggca ttggtcacat tctcacgact ttccgccata aactgagtaa cattagacag
    39601 tttagccccc ttaccagtac gagggagatt aacactcatg ccgcgctcaa attcgccaga
    39661 tacgatacgg acaaaggcaa tacggtcacg gtgacgaggg tccatgttgg cttggatttt
    39721 aaagacaaag cctgagaaat ccttgtcata aggatccaca atttcaccgt ctgttttctt
    39781 gtgaccatgt ggttctggag caaacttgag gaaggtttca aggaaggtct gcacaccaaa
    39841 gtttgtcagg gctgaaccga aaaagacagg cgtcaattct ccagccagaa tagcttcctc
    39901 tgaaaactca ttcccagctt catttaaaag ctcaatgtca tccttgactt gctcgtaaaa
    39961 aggattgcta ccaaaaagtt tgtccccatc ttctaggcta gcaaaacgct catccccttt
    40021 ataaagttct aaacgttggt tatagaggtc atacaagccc tcaaaggctt tccccatccc
    40081 gataggccag ttcatagggt agctagcaat gcccaagatt tcttccaatt cttgcaagag
    40141 atccaaaggc tcacgaccgt cacggtccag cttgttcata aaggtaaaaa ctggaatgcc
    40201 acgatgtttc acaacctcaa acaatttctt ggtttgagcc tcgatcccct tggcagagtc
    40261 cacgaccatg accgcagcat ccaccgccat caaggtacga taggtatctt ctgagaagtc
    40321 ctcgtgccct ggcgtgtcta agatattcac gcgcttgccg tcgtagtcaa attgcataac
    40381 agatgaagta acagaaatcc cacgttgctt ctcgatatcc atccagtcag atttagcaaa
    40441 agtccctgtt ttcttccctt ttaccgtacc agcctcacga atctcacccc caaagtagag
    40501 taactgctca gtgatggttg ttttccccgc gtccgggtgg gagataatgg caaaggtacg
    40561 acgtttctta atttcttctt gaatattcat aagttctctt tctttgattc tctatttttc
    40621 ttgtttcaat agctgagaat gatttttaca ttggatttta ccattccttt caacactcca
    40681 ttatatcgga ttttagcatt tttttcaatt tctatttctt ttcacttccc cctcccttat
    40741 ttataggaaa atatggtaaa atagaacaga ctaaaaatca tcatttcacg aaaggatgca
    40801 agatgaaaat tacgcaagaa gaggtaacac acgttgccaa tctttcaaaa ttaagattct
    40861 ctgaagaaga aactgctgcc tttgcgacca ccttgtctaa gattgttgac atggttgaat
    40921 tgctgggcga agttgacaca actggtgtcg cacctactac gactatggct gaccgcaaga
    40981 ctgtactccg ccctgatgtg gtcgaagaag gaacagaccg tgatcgcttg tttaaaaacg
    41041 tacctgaaaa agacaactac tatatcaagg tgccagctat cctagacgat ggaggagatg
    41101 cctaatgact tttaacaata aaactattga agagttgcac aatctccttg tctctaagga
    41161 aatttctgca acagaattga cccaagcaac acttgaaaat atcaagtctc gtgaggaagc
    41221 cctcaattca tttgtcacca tcgctgagga gcaagctctt gttcaagcta aagccattga
    41281 tgaagctgga attgatgctg acaatgtcct ttcaggaatt ccacttgctg ttaaggataa
    41341 catctctaca gacggtattc tcacaactgc tgcctcaaaa atgctctaca actatgagcc
    41401 aatctttgat gcgacagctg ttgccaatgc aaaaaccaag ggcatgattg tcgttggaaa
    41461 gaccaacatg gacgaatttg ctatgggtgg ttcaggtgaa acttcacact acggagcaac
    41521 taaaaacgct tgggaccaca gcaaggttcc tggtgggtca tcaagtggtt ctgccgcagc
    41581 tgtagcctca ggacaagttc gcttgtcact tggttctgat actggtggtt ccatccgcca
    41641 acctgctgcc ttcaacggaa tcgttggtct caaaccaacc tacggaacag tttcacgttt
    41701 cggtctcatt gcctttggta gctcattaga ccagattgga ccttttgctc ctactgttaa
    41761 ggaaaatgcc ctcttgctca acgctattgc cagcgaagat gctaaagact ctacttctgc
    41821 tcctgtccgc atcgccgact ttacttcaaa aatcggccaa gacatcaagg gtatgaaaat
    41881 cgctttgcct aaggaatacc tcggtgaagg aattaaccca gaggttaagg aaaccattct
    41941 aaatgccgct aaacactttg aaaaattggg tgctattgtc gaagaagtca gccttcctca
    42001 ctctaaatac ggagttgccg tatactacat catcgcttca tcagaagctt catcaaactt
    42061 gcaacgtttt gacggtatcc gttacggcta ccgcgcagaa gatgcaacca accttgatga
    42121 aatctatgta aacagccgaa gccaaggttt tggtgaagag gtaaaacgtc gtatcatgct
    42181 gggtactttc agtctttcat caggttacta tgatgcctac tacaaaaagg ctggtcaagt
    42241 ccgtaccctc atcattcaag atttcgaaaa agtcttcgca gattacgatt tgattttggg
    42301 tccaactgct ccaagtgttg cctatgactt ggactcactc aaccatgacc cagttgccat
    42361 gtacttggct gacctcttga ccatacctgt aaacttagca ggtctccctg gaatttcgat
    42421 tcctgctgga ttctctcaag gtctacctgt cggtctgcaa ttgattggtc ccaagtactc
    42481 tgaggaaacc atttaccaag ctgctgctgc ttttgaagca acaacagact accacaaaca
    42541 acaacccgtg atttttggag gtgacaacta atgaactttg aaacagtcat cggacttgaa
    42601 gtccacgtag agctcaacac caattcaaaa atcttctcac ctacttctgc ccactttgga
    42661 aatgaccaaa atgccaacac taacgtgatt gactggtctt tcccaggagt tctaccagtt
    42721 ctcaataaag gggttgttga tgccggtatc aaggctgctc ttgccctcaa catggacatc
    42781 cacaaaaaga tgcactttga ccgcaagaac tacttctatc ctgataaccc caaagcctac
    42841 caaatttctc agtttgatga gccaatcggt tataaaggct ggattgaagt cgagctagaa
    42901 gacggtacga ccaagaaaat cggtatcgaa cgtgcccacc tagaggaaga cgctggtaaa
    42961 aacacccatg gtacagatgg ctactcttat gttgacctca accgccaagg ggttcccttg
    43021 attgagattg tatctgaggc agatatgcgt tctcctgaag aagcctatgc ttatctgaca
    43081 gccctcaagg aagttatcca gtacgctggc atttctgacg ttaagatgga ggaaggttcg
    43141 atgcgtgtgg atgccaacat ctcccttcgt ccttatggtc aagagaaatt cggtaccaag
    43201 actgaattga agaacctcaa ctccttctca aacgttcgca aggggcttga atacgaagtc
    43261 caacgtcaag ctgaaatact tcgctcaggt ggtcaaatcc gccaagaaac acgccgttac
    43321 gatgaagcta acaaagcaac catcctcatg cgtgtcaagg aaggggctgc tgactaccgc
    43381 tacttcccag aaccagacct gcctctcttt gaaatctcag acgagtggat tgaggaaatg
    43441 cggactgagt tgccagagtt tccaaaagaa cgtcgtgcgc gttatgtatc tgaccttggt
    43501 ttatcagact acgatgctag tcagttgact gctaataaag tcacttctga cttctttgaa
    43561 aaagctgttg ccctaggtgg tgatgccaaa caagtctcta actggctcca aggggaagtc
    43621 gctcagttct tgaatgctga aggtaaaaca ctggaacaaa tcgaattgac accagaaaac
    43681 ttggttgaaa tgattgccat catcgaagac ggtactattt catctaagat tgccaagaaa
    43741 gtctttgtcc atctagctaa aaatggcggt ggcgcgcgtg aatacgtgga aaaagcaggt
    43801 atggttcaaa tttcagatcc agctatcttg atcccaatca tccaccaagt ctttgccgat
    43861 aacgaagctg ctgttgccga cttcaagtca ggcaaacgta acgccgacaa agccttcaca
    43921 ggattcctta tgaaggcaac caaaggccaa gccaacccac aagttgccct taaactactt
    43981 gcacaggaat tggcgaagtt gaaagaaaac tagacagaac aaaaccagcc ctaaggttgg
    44041 ttttttcttg acaaatagaa agaaaacgtt tacaatattg cgtgtaaagt ttccaaacaa
    44101 gaaaactaaa aatcgcaaca ataaggagtt atttatatgg ctacaatgaa ggcagctcgc
    44161 tggcatgcag cgaaagatgt tcgtattcaa gaaatagaag ttcctgaagt acttccgcac
    44221 caagtaaaag ttgcagttaa gttcacagga atttgtggta ctgacctcca tgaattttta
    44281 gatggaccta tctttatccc aacagatgag cacgtctatt ctggtcaaaa agcaccggta
    44341 acactgggac atgaattttc tggtgaaatc atagaagtcg gaagcgatgt tacccgtgtt
    44401 aaagttggtg atcgtgttgc tgtggaacca attctagcta aaaataactt agttggtgat
    44461 tataatttgg atccaaacct taattttgtc ggtttggctg cagacggtgg atttgccaaa
    44521 tattgtgttt tagacggtga cttagtacat gtcattccag atagcttgag ctatgagcaa
    44581 gctgctctta ctgaacccgc tgctgttgca gtctatgctg ttcgtcaatc ttctttgaag
    44641 gctggcgata cagctgttgt ctttggttta ggcccgattg gtctattaat tgtagaagct
    44701 cttcgtgcag ctggtgcttc taagatttat gctgttgaac tatcacctga acgtcaagca
    44761 aaagctgaag agttaggagc tatcgttgtc cgtccggaag aaggtgaaac agctgttgaa
    44821 gctattcatc gtttaacaaa cggtggtgta gatgtttctt atgaagtaac gggtgtgcca
    44881 gtagttttag gacaagcctt agctgccgta cataaagcag gtgagtgcat ggtggtctct
    44941 atctgggaac gagaagcaaa tattaatccg aatgaatttg caatccaaga aaagacacta
    45001 aaaggaatca ttgcttatcg tcatatcttc cctaaagtat tagaattgat ggaacaaggc
    45061 tacttctctg ctgaaaaatt agttactaag aaaattaaat tggaaaatat cgtcgaagaa
    45121 ggctttatcg aattaactca agataagtca caaattaaaa ttttggtaga accagaataa
    45181 aaaatcaatc tttttaagac aatactaaca ggtcggagta aacttacttc ggccttttga
    45241 aatatagtac tcttcgaaaa tctcttcaaa ccacgtcagc gtcacattac cgtatatatg
    45301 gttactgact tcgtcagttc tatccacaac ctcaaaacag tgttttgagc agcctgcggc
    45361 tagcttccta gtttgctctt tgatttccat tgagtatgag aagaaatata gtggatactc
    45421 tttaacatta accactccac ctgactagcc aatattcttt ttcgctccta ccaactccca
    45481 ataactattt tggctttatt tccagagtat tttatggtaa aatgaagagt aataatattt
    45541 gttaaagagg taaaaacatg attgaagcaa gtaaattaaa agctggtatg acctttgaaa
    45601 cagctgacgg caaattgatt cgcgttttgg aagctagtca ccacaaacca ggtaaaggaa
    45661 acacgatcat gcgtatgaaa ttgcgtgatg tccgtactgg ttctacattt gacacaagct
    45721 accgtccaga ggaaaaattt gaacaagcta ttatcgagac tgtcccagct caatacttgt
    45781 acaaaatgga tgacacagca tacttcatga atacagaaac ttacgaccag tacgaaatcc
    45841 ctgtagtcaa tgttgaaaac gaattgcttt acatccttga aaactctgat gtgaaaatcc
    45901 aattctacgg aactgaagtg atcggtgtca ccgttcctac tactgttgag ttgacagttg
    45961 ctgaaactca accatctatc aaaggtgcta ctgttacagg ttctggtaaa ccagcaacga
    46021 tggaaactgg acttgtcgta aacgttccag acttcatcga agcaggacaa aaactcgtta
    46081 tcaacactgc agaaggaact tacgtttctc gtgcctaatc tctagaaaga ggtcattcta
    46141 tgggaattga agaacaactt ggcgaaatcg ttatcgcccc acgtgtactt gaaaaaatca
    46201 ttgctatcgc tactgcaaag gtagagggtg ttcactcttt ttcaaacaga tcagtgtctg
    46261 ataccctttc aaaactttca ctcggccgtg gcatttatct taaaaacgtg gacgaagaac
    46321 tcacagcaga tatctatctc taccttgagt acggagtaaa agttcctaag gtagcggttg
    46381 ctatccagaa agctgtcaaa gatgccgtcc gtaatatggc tgatgtagaa ctcgctgcta
    46441 tcaatattca cgttgcaggt atcgtcccag ataaaacacc aaaaccagaa ttgaaagatc
    46501 tatttgacga ggacttcctc aatgactagt ccactattag aatctagacg ccaactccgt
    46561 aaatgcgctt ttcaagctct catgagcctt gagttcggta cggatgtcga aactgcttgt
    46621 cgtttcgcct atactcatga tcgtgaatat acggatgtac aacttccagc ctttttgata
    46681 gacctcgttt ctggtgttca agctaaaaag gaagaactag ataagcaaat cactcagcat
    46741 ttaaaagcag gttggaccat tgaacgctta acgctcgtgg agagaaacct ccttcgcttg
    46801 ggagtctttg aaatcacttc atttgacact cctcagctgg ttgctgttaa tgaagctatc
    46861 gagcttgcaa aggacttctc cgatcaaaaa tctgcccgtt ttatcaatgg actgctcagc
    46921 cagtttgtaa cagaagaaca ataaggctct ttgtcaactg tagtgggttg aagaaaagct
    46981 aagctcgaga aaggacaaat ttcgtccttt cttttttgat gttcagagcg atgaaaatcc
    47041 gttttttgaa gttttcaaag ttccgaaaac caaaggcatt gcgcttgata agtttgatga
    47101 gattattggt cgcttccagt ttggcattag aatagtgtag ttgaagggcg ttgacaatct
    47161 tttctttatc tttgaggaag gttttaaaga cagtctgaaa aataggatga acctgcttaa
    47221 gattgtcctc aataagtccg aaaaatttct ctggttcctt attctggaag tgaaaaagca
    47281 agagctgata gtggtgatag tggtgtttca agtcttccga ataactcaaa agcttgttta
    47341 aaatctcttt attggttaag tgcatacgaa aagtaggacg ataaaaacgt ttatcactca
    47401 gtttacggct atcctgttga atgagtttcc agtagcgctt gatagccttg tattcatggg
    47461 attttcgatg aaactgattc atgatttgga cacgcacacg actcatagca cggctaagat
    47521 gttgtacaat gtgaaagcga tcaagaacga ttttagcatt cgggagtgaa acagtctggg
    47581 agactgtttc agcctgagcc tagaaatttg aaagcgaagc tgtttagcca agtcatagta
    47641 agggctaaac atatccatag taatgatttt cacttgacaa cgaacggctc tatcgtagcg
    47701 aagaaagtga tttcggatga cagcttgtgt tctaccttca agaacagtga tgatatcgag
    47761 cttttcaaaa tcttgcgcaa taaagctcat ctccatctcc cgattgaaac agtcactccc
    47821 tggactgttt caacgtccca agacataatc ttaggcagat ttctaaaatt acactcaaag
    47881 tggaagtcat tgagctttcg aatgacagtt gaagttgaga tggaaagctg atgggcaata
    47941 tcagtcatag aaatcttttc aattaacttt tgagcaatct tttggttgat gatacgaggg
    48001 atttgatgat tcttcttgac gatagaagtt tcagcgacca tcatttttga acagtgatag
    48061 cacttgaatc gacgctttct aaggagaatt ctagtaggca taccagttgt ttcgaggtaa
    48121 gggatcttag acggtttttg aaagtcatat ttcttcaatt ggtttccgca ctca
//
LOCUS       DAVKQL010000006        47977 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVKQL010000006 DAVKQL010000000
VERSION     DAVKQL010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 47977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47977
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.006"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            269..1036
                     /locus_tag="VW561_000272"
     CDS             269..1036
                     /locus_tag="VW561_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9942608.1"
                     /translation="MSQKLNHLDVGEFVLLLPEHLRSEEEHYKSVFEDDLTSRMSSQD
                     ERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQLTGPQSILFWIDAVQ
                     NYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVMLAGAVLGIAT
                     SILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLGFVASVFLQVE
                     ILVAFLVSLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
     gene            1038..1670
                     /locus_tag="VW561_000273"
     CDS             1038..1670
                     /locus_tag="VW561_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9942609.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLESYDGMIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLAYLDLDKRIFELSGGESQRVALAKVILKNPPLILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            1811..2089
                     /locus_tag="VW561_000274"
     CDS             1811..2089
                     /locus_tag="VW561_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComA"
                     /protein_id="HEU9942610.1"
                     /translation="MKKFDNYIIEKPCDSNSDKLQKILIIENLVDDILQFPLRINNSV
                     GEIFLLQPFQKKTIFIPCYFEEDIVKVKDDDKVEWNLLEFQKFRAFLA"
     gene            complement(2598..3011)
                     /gene="ndk"
                     /locus_tag="VW561_000275"
     CDS             complement(2598..3011)
                     /gene="ndk"
                     /locus_tag="VW561_000275"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU9942611.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYLPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEVIQNVVHGSDSEESAKREIALWF"
     gene            complement(3122..6799)
                     /gene="rpoC"
                     /locus_tag="VW561_000276"
     CDS             complement(3122..6799)
                     /gene="rpoC"
                     /locus_tag="VW561_000276"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU9942612.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKTEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(6832..10443)
                     /gene="rpoB"
                     /locus_tag="VW561_000277"
     CDS             complement(6832..10443)
                     /gene="rpoB"
                     /locus_tag="VW561_000277"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU9942613.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TSEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVIAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            10859..10929
                     /locus_tag="VW561_000278"
     tRNA            10859..10929
                     /locus_tag="VW561_000278"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10891..10893,aa:Cys,seq:gca)
     gene            complement(11127..12458)
                     /locus_tag="VW561_000279"
     CDS             complement(11127..12458)
                     /locus_tag="VW561_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEU9942614.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTNTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(12687..13511)
                     /gene="endA"
                     /locus_tag="VW561_000280"
     CDS             complement(12687..13511)
                     /gene="endA"
                     /locus_tag="VW561_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEU9942615.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMPSAPNSPKTNLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(13550..13738)
                     /locus_tag="VW561_000281"
     CDS             complement(13550..13738)
                     /locus_tag="VW561_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU9942616.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            complement(13731..15014)
                     /gene="murA"
                     /locus_tag="VW561_000282"
     CDS             complement(13731..15014)
                     /gene="murA"
                     /locus_tag="VW561_000282"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU9942617.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(15038..15268)
                     /locus_tag="VW561_000283"
     CDS             complement(15038..15268)
                     /locus_tag="VW561_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEU9942618.1"
                     /translation="MVQLLFTLSSHMFFIYVSFYLLKDLVRWEKVLKVTDDNTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(15326..16363)
                     /locus_tag="VW561_000284"
     CDS             complement(15326..16363)
                     /locus_tag="VW561_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU9942619.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKEILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(16347..16835)
                     /gene="coaD"
                     /locus_tag="VW561_000285"
     CDS             complement(16347..16835)
                     /gene="coaD"
                     /locus_tag="VW561_000285"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU9942620.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATFLVRGLRNAADLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACHVPESILEEIRNEK
                     KD"
     gene            complement(16825..17364)
                     /gene="rsmD"
                     /locus_tag="VW561_000286"
     CDS             complement(16825..17364)
                     /gene="rsmD"
                     /locus_tag="VW561_000286"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEU9942621.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAKRELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(17429..18421)
                     /locus_tag="VW561_000287"
     CDS             complement(17429..18421)
                     /locus_tag="VW561_000287"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEU9942622.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(18532..19215)
                     /locus_tag="VW561_000288"
     CDS             complement(18532..19215)
                     /locus_tag="VW561_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEU9942623.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYLLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(19271..20011)
                     /locus_tag="VW561_000289"
     CDS             complement(19271..20011)
                     /locus_tag="VW561_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU9942624.1"
                     /translation="MTIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRNQLAAFPQTVWISEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVATGILIFHLS"
     gene            20045..20323
                     /locus_tag="VW561_000290"
     CDS             20045..20323
                     /locus_tag="VW561_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEU9942625.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            20401..21327
                     /gene="yidC"
                     /locus_tag="VW561_000291"
     CDS             20401..21327
                     /gene="yidC"
                     /locus_tag="VW561_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU9942626.1"
                     /translation="MKSIKRFAFSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(21402..22196)
                     /gene="pflA"
                     /locus_tag="VW561_000292"
     CDS             complement(21402..22196)
                     /gene="pflA"
                     /locus_tag="VW561_000292"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEU9942627.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(22322..23572)
                     /locus_tag="VW561_000293"
     CDS             complement(22322..23572)
                     /locus_tag="VW561_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEU9942628.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(23639..24466)
                     /gene="purR"
                     /locus_tag="VW561_000294"
     CDS             complement(23639..24466)
                     /gene="purR"
                     /locus_tag="VW561_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEU9942629.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(24733..25674)
                     /locus_tag="VW561_000295"
     CDS             complement(24733..25674)
                     /locus_tag="VW561_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEU9942630.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMSNKIFAMDNRSFYKPDLD"
     gene            complement(25664..26920)
                     /locus_tag="VW561_000296"
     CDS             complement(25664..26920)
                     /locus_tag="VW561_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEU9942631.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVAVSDRLQEVRLELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYEVGDKDEIERCRKSLLASVKRFARDIRNKYI
                     APPRTTNFGILFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(26922..27584)
                     /locus_tag="VW561_000297"
     CDS             complement(26922..27584)
                     /locus_tag="VW561_000297"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEU9942632.1"
                     /translation="MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQYFVQARPEKDDTDLELALLTIFEQNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMRQIEIEDGQNLIAYCSEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(27547..28203)
                     /locus_tag="VW561_000298"
     CDS             complement(27547..28203)
                     /locus_tag="VW561_000298"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU9942633.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTPVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGEVNERVQTLRKQLD
                     "
     gene            complement(28214..29092)
                     /gene="rsgA"
                     /locus_tag="VW561_000299"
     CDS             complement(28214..29092)
                     /gene="rsgA"
                     /locus_tag="VW561_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEU9942634.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(29094..29966)
                     /gene="rsmA"
                     /locus_tag="VW561_000300"
     CDS             complement(29094..29966)
                     /gene="rsmA"
                     /locus_tag="VW561_000300"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEU9942635.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            complement(30097..30405)
                     /locus_tag="VW561_000301"
     CDS             complement(30097..30405)
                     /locus_tag="VW561_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942636.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFERMFRENC
                     "
     gene            complement(30398..31039)
                     /locus_tag="VW561_000302"
     CDS             complement(30398..31039)
                     /locus_tag="VW561_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9942637.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(31041..33071)
                     /locus_tag="VW561_000303"
     CDS             complement(31041..33071)
                     /locus_tag="VW561_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9942638.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGEGKLPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSVYYQGLFTDYL
                     QNFSSESVEVTSQKHYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GARPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRDLMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(33148..33444)
                     /locus_tag="VW561_000304"
     CDS             complement(33148..33444)
                     /locus_tag="VW561_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEU9942639.1"
                     /translation="MKQTVKKLALVASIAATLGGSVAVASAAVQYPEGGVWTYGSGNG
                     GAYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     gene            complement(33764..34663)
                     /locus_tag="VW561_000305"
     CDS             complement(33764..34663)
                     /locus_tag="VW561_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9942640.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLAYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMPQIIEKEAYSINDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTQIENYFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALRVERDFSLAESFYQSSKTFAK
                     LIGDGFLVKKLTEEWQEDVKKYL"
     gene            complement(34735..35295)
                     /gene="rnmV"
                     /locus_tag="VW561_000306"
     CDS             complement(34735..35295)
                     /gene="rnmV"
                     /locus_tag="VW561_000306"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEU9942641.1"
                     /translation="MKERISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(35295..36068)
                     /locus_tag="VW561_000307"
     CDS             complement(35295..36068)
                     /locus_tag="VW561_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU9942642.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIE
                     HALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(36374..37039)
                     /locus_tag="VW561_000308"
     CDS             complement(36374..37039)
                     /locus_tag="VW561_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEU9942643.1"
                     /translation="MTILGKDTVQQSAKGESVTQEATPEYKLENTPGGDKGGNTGSSD
                     ANANEGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKNAIEKAAKNKQDEIKGAPL
                     SDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGVQAIAMVTVPKRPVAPNAA
                     PKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALASLGLVVATSGFALLGRKTR
                     RRK"
     gene            complement(37302..37436)
                     /gene="rpmH"
                     /locus_tag="VW561_000309"
     CDS             complement(37302..37436)
                     /gene="rpmH"
                     /locus_tag="VW561_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEU9942644.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            complement(37581..38795)
                     /locus_tag="VW561_000310"
     CDS             complement(37581..38795)
                     /locus_tag="VW561_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9942645.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(38951..39244)
                     /locus_tag="VW561_000311"
     CDS             complement(38951..39244)
                     /locus_tag="VW561_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942646.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVEVIMICQLESLVPLLYPSFIG
                     FLVFHVLYHLILFLVAKRSGRWDYLMIWGLFLMFNLLYDSFLGLLFLGLSFGM"
     gene            39406..39858
                     /locus_tag="VW561_000312"
     CDS             39406..39858
                     /locus_tag="VW561_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU9942647.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(39893..41281)
                     /locus_tag="VW561_000313"
     CDS             complement(39893..41281)
                     /locus_tag="VW561_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9942648.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAADKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            41347..42309
                     /locus_tag="VW561_000314"
     CDS             41347..42309
                     /locus_tag="VW561_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEU9942649.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVNTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(42338..42541)
                     /locus_tag="VW561_000315"
     CDS             complement(42338..42541)
                     /locus_tag="VW561_000315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942650.1"
                     /translation="MNTIERTRRLVKGCATHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     SKHVFEVVDRTDEVSNHTYGKAT"
     gene            complement(42605..43615)
                     /gene="ccpA"
                     /locus_tag="VW561_000316"
     CDS             complement(42605..43615)
                     /gene="ccpA"
                     /locus_tag="VW561_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEU9942651.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGIAYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(44040..44639)
                     /locus_tag="VW561_000317"
     CDS             complement(44040..44639)
                     /locus_tag="VW561_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9942652.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSERLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSVADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(44641..45738)
                     /locus_tag="VW561_000318"
     CDS             complement(44641..45738)
                     /locus_tag="VW561_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9942653.1"
                     /translation="MLERLKSIHYMFWASLIFMLFPILPVVIGELPAWHLLVDILFVV
                     TYLGVLITKNQRLSWLFWGLMLVYVAGNTAFVAGNYIWFFFFLSNLLIYHFGVRSLKS
                     LHVWTFLLAQVLVVGRLLIFQRIEVEFLVYMLVILTFVDLMTLGSVRIRLVEDLKEAQ
                     VKQNTQINLLLAENERNRIGQDLHDSLGHTFAMLSVKIDLALQLFQMQAYPQVEKELR
                     EIQQISKESMREVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     ESMASMILLELVTNIIKHAKASKAYLKLERTEKELILTVSDDGCGFAFLKGDELHTIR
                     DRVFPFSGEVSVISQKHPTEVQVRLPYKERN"
     gene            complement(45738..46475)
                     /locus_tag="VW561_000319"
     CDS             complement(45738..46475)
                     /locus_tag="VW561_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9942654.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GELANFMRDYLISMTAFSMMSTTILSFPAVLHTDKMNNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFTVGHFVRGVDMPLVKWVGAAILLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAVGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(46477..47361)
                     /locus_tag="VW561_000320"
     CDS             complement(46477..47361)
                     /locus_tag="VW561_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9942655.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLSNQEIDQLLQFVKQQKEQLAEKLSGGQKRLFSFVLTLIGRPKIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            complement(47348..47542)
                     /locus_tag="VW561_000321"
     CDS             complement(47348..47542)
                     /locus_tag="VW561_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942656.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTDWFDQALLRQFIRGALGFGFM
                     IFVVFLMGMEWLKGESHDGG"
     gene            complement(47539..47745)
                     /locus_tag="VW561_000322"
     CDS             complement(47539..47745)
                     /locus_tag="VW561_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942657.1"
                     /translation="MGEEEMRNKMIIAVSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
BASE COUNT        13802 a        11031 c         9114 g        14030 t
ORIGIN      
        1 ttaatttaat tgagttggaa gagagaattc aaactactat tggaattaaa caaataaaca
       61 cacacaatat tattactatt gcccgagaag ggtactctca aaattattta cctaacactt
      121 cagaaaatac atataattca ttacaagtca gtttagttgg agtattacta ctttttataa
      181 gtatggtaaa tattttatgg gctaaaaaaa gtaaatgaaa ataaaatttg gagccctctg
      241 aaaaagtaag tcctacagtt caactaaaat gagtcaaaag ttgaatcacc ttgatgtagg
      301 ggagtttgtc ttattgctgc ctgaacacct ccgttcagag gaagaacatt ataaatctgt
      361 ttttgaagac gacttaacca gtcgcatgtc tagtcaagat gaacgacagc aaatgactgc
      421 tacggtaggt tatttagaat caggtcagga tcgttttgtg tataatacga cccctatttc
      481 ttaccagcag tttttgaaag atccaatcat cattgttata acaccccaat taactggtcc
      541 acagtccatt ttgttttgga tagacgcagt acagaactac gttctcttta atcaattgtc
      601 tgatgcccag gagcttatcc agagacaagg cattgaaaat tgggtctcag aaatgcaaac
      661 aggttaccac aactacatca cattattgga taatatccag agggaacgtt gggtaatgct
      721 agcaggagct gtgcttggga ttgcaacttc aatcttgttg tttaacacta tgaataggct
      781 ctactttgaa gaatttagac gtgccatttt tatcaaacgc attgcaggtc tcaggttctt
      841 agaaatccat cgcacttatc tctttgctca actgggtgtg tttttactgg gatttgttgc
      901 gagtgtattt cttcaggtag agatactagt tgctttctta gtctcgttac tctttactgg
      961 tctatctctt ttacagttac atgtccaaat gcagaaagaa aacaagatgt ccatgcttgt
     1021 tttgaaggga ggttaatatg attgaactta aacaggtgag taaatctttt ggagaacgag
     1081 agttattttc gaatctttca atgacatttg aggctggaaa agtctatgcc ttaattggtt
     1141 caagtggtag cggaaaaaca accttgatga acatgattgg gaaattagaa tcttatgatg
     1201 ggatgatttt ttaccgaggt aaagacttgg ccaattataa atcgagtgat tttttccgtc
     1261 acgaattggg ctacctcttc cagaactttg gcttaattga aaaccaaagt attgaagaaa
     1321 accttaagct aggtctcatt ggtcaaaagt tgagtcggtc ggaacagcgg ttgaggcaga
     1381 agcaggcttt agaacaggtc ggcctggctt atcttgacct agataagcgc atctttgagt
     1441 tatcgggcgg agaatcgcaa cgggttgcct tggcaaaagt tatcttaaag aatccacccc
     1501 ttattctggc agatgagcca acagcttcaa tagacccagc aacctctcag ttgattatgg
     1561 agattttgct atctcttcga gatgataata ggctaatcat tatcgcaaca cataatccgg
     1621 caatttggga gatggctgat gaagtgttca cgatggatca tctgaaataa aaatccttgt
     1681 ttttaattgc acgatgagtt actgaaatat tatcatgaat caataattgg agttaattta
     1741 gaattgtact ttattaatat tgaggtaact ttttcttgat aaaggaggaa atagtggaga
     1801 ggaagttaga atgaaaaaat tcgacaatta tattattgag aagccttgcg attctaattc
     1861 agataaactg caaaaaatct taataattga aaatttggta gatgatattt tgcaatttcc
     1921 tctcagaatc aataatagtg taggagagat tttcctccta caaccgtttc aaaagaaaac
     1981 tatctttatt ccatgttatt ttgaggaaga tattgtgaaa gtcaaagatg atgataaagt
     2041 tgagtggaat ttgttagaat ttcaaaaatt tagagcattt ttggcttagt aatctgtgtt
     2101 gaaggctcaa aacctatggt aaaaaagtcg ctttgaaaac gtattgcctc caaagattta
     2161 gttaaataat gatttaacac aaaaagaaat tattgaagtt ctggaaagat gttgtttcag
     2221 tattgagaaa aggtgggaaa aacttgcgat tttcacagag aaaggaagaa aaagtataga
     2281 aatatagtca attgaaacaa gaacaggaca aaagaacctt ttgtgccata tttttctcct
     2341 ttcgctttac aattggcttg aacaccttta ttgtatcgcg tttggagttt ttttggtata
     2401 accttcgacg cgcacccgca tagcgggtgt ttttttgtct cgcacctaac ggagcgagac
     2461 gaactaatag tcacttaatc aaaaaacgca ccatatcaaa aactaaaaag tttgatatca
     2521 tgcgtcatgt cttaaactaa ttgactatac tttctattca aatgagcttt taaccaattg
     2581 attgagccaa tccactctta aaaccaaaga gcaatttctc gcttagctga ctcttctgaa
     2641 tctgaaccat gtacaacatt ttggataacc tcattttctc cagcagcttt tgcaaagtca
     2701 cctcgaatag tgcctggtaa agcttcttct ggacgagttg cacccatcat ggtccgccaa
     2761 gtttcgatta ctttgggacc agaaatgaca cccacaagaa ctggacctga agtcatgaat
     2821 tcacgaatcg gtaggtaaaa actctgacca accaagtcct gatagtgctg gtcaatcaac
     2881 tcttctgaaa cctgtgaacg aaactccaat ttttcgattg taaatccacg ttgttcgatg
     2941 cgctttaaca cttcacccac tagccctctt tttacaccat ctggtttgat gataaagaat
     3001 gtttgttcca tacccgtctc ctttgtcagc ttctttcttt tattttacca catttcgtgg
     3061 aaaaatggag aaagttttca gaagagagaa tgagagaacc ctcgggttct ctcattctct
     3121 cttattctac tgtttcttcc acagtttcaa cggcagtatc cacaactact tctgttgttt
     3181 cttcatttcc ttcttcctct actggaggat taaggtattc ttcttcgttg acagcatgtg
     3241 gttcaaggtt acggtaacgg gccataccag taccagctgg gatgatctta ccgatgataa
     3301 cattttcttt aagtccaagg agatggtctt tcttaccacg gatagctgcg tcagtaagga
     3361 cacgagttgt ttcttggaag gaagctgctg acaagaaact gtttgtttca agtgaggctt
     3421 tggtaattcc cataaggact gggcgacctg tcgctggaac tccacctgcg ataaggacat
     3481 ctttgttggc atctgtaaag tcattgatat ccatgagggt acccatgaga agatctgtat
     3541 cacctggatc catgacacgg actttacgga tcatttgacg aaccattacc tcgatgtgtt
     3601 tgtcaccgat ttctacccct tggctacggt aaactttttg tacttcaccg agaaggtacg
     3661 tttcaactga caagacatca cgaactgcaa ggagacgttt tggttggata gaaccttctg
     3721 tcagagcagc accacgcgct acttggcccc caacttcgac acgcatacga gctgtaaatg
     3781 gaacgacata ttcaccttcg ccagtttcac ccttaacaaa gactttcttg gtacgagttg
     3841 atgcatcttc ttcgatagca gtaacttgtc ctttaacctc tgtgataacc gcttcccctt
     3901 taggattacg ggcttcaaag atttcttgga cacgaggaag accctgagtg atatcggtat
     3961 ttgaggcaac cccacctgtg tggaaggtac gcattgtaag ctgtgtacca ggttccccga
     4021 tagattgggc agcaattgta ccaactgctt caccaacttc aaccgcatca ccagtcgcca
     4081 agttgatacc gtaacagtga cggcagacac cgtgacgagt gttacatgta aatacagaac
     4141 ggatagtcac ttcttccaca ccagcattga caatttcacg cgccttgtct tctgtaatca
     4201 attcatttgg accaataatc actgcaccag tttctggatg tttaacagtt ttcttagtgt
     4261 aacgaccgtt gagacgctct tcgagagact cgatcatctc ttttccttct gcgatagaac
     4321 ggatcaagag accacggtca gttccacagt cgtcctcacg gatgataacg tcttgggcaa
     4381 cgtcgaccaa acgacgagtc aagtaacctg agtcggctgt cttaagggcc gtatcggtca
     4441 tacctttacg agcaccgtga gttgagaaga acatttccaa taccgacaaa ccttcgcgga
     4501 agtttgaaag gattggcaat tccatgatac gtccattcgg agcagccatc agaccacgca
     4561 taccggcaag ctgtgagaag tttgagatgt taccacgggc tccagagtcc atcatcataa
     4621 cgattgggtt cttaggatct tggttggcaa tcaagcgttt ctcaagtttt tcacgggcag
     4681 cacgccattc agctgtaaca gcattgtaac gctcgtcgtc tgtgatcata ccacgacgga
     4741 attgtttggt gatttgttcg acacgtttgt gtgattcttc aatgatttca gtcttgtcat
     4801 caacgactgg gatatcggca atacccactg tcaatcctgc aagagttgag tggtggtaac
     4861 cgaggttctt catgcggtca agtagggcag aagtttctgt cgtacggaaa cgtttgaaga
     4921 tttcagcgat gatatttcca aggtttttct tcttgaatgg agggttgagc tcaagattgc
     4981 tgatagcttc cttgatatct ccaccaagtg gcaagaagta tttagctgga acaccttctg
     5041 tcaagttggc attgtttggt tcttgcaagt atggtagccc ctctggcatg atatcgttga
     5101 agagaatttt accaactgtt gtaagcaaga ccttatgtct ttgctcttct gtccaaggct
     5161 tgttgaggct gtctgttgcg ataccaacac gtgagtggag gtgaacataa ccattgcggt
     5221 aagccataac cgcttcgtca cggtctttga agaccattcc ttcaccttcg cgaccagctt
     5281 cttccatggt caagtagtag ttacccaaaa ccatgtcctg agatggagta actaccggtt
     5341 tcccatcttt cgggttcaag atgtgctcag cagctagcat gaggatacga gcttctgctt
     5401 gtgcttcttc tgaaagtggt acgtggatgg ccatttggtc cccgtcaaag tcagcattgt
     5461 aggcttcaca gacaagtggg tgcaagcgaa gagccttacc atcaattaag actggctcga
     5521 aggcttggat acccaaacgg tgaagggtcg gtgcgcggtt caaaagcact gggtgttctt
     5581 taatcacttc ttcaaggata tcccagatac gctcatctcc gcgttccacc aagcgtttag
     5641 ctgctttgac gttttgcacg atatcacggg caacgatttc acgcatgaca aatggtttaa
     5701 agagttcaat cgccatttca cgtggcacac cacattggta catcttaaga gttggaccaa
     5761 cggcgataac ggaacgtcct gagaagtcaa cacgtttacc gagcaagttt tgacggaagc
     5821 gtccttgttt acctttaagc atgtggctca atgatttcaa tggacggcta cctggtcctg
     5881 tgattggacg accacgacga ccattgtcaa tcaaagcgtc aactgcttct tgaagcatac
     5941 gcttctcatt ttgaacgatg atacctggtg catttaactc aagcaaacga gccaaacggt
     6001 tgttacggtt gataacacgg cggtaaaggt cattcaagtc agatgaggca aaacggccac
     6061 catccaactg caacattgga cgaagatctg gtgggataac cggaaggatg ttaagaatca
     6121 tccattcagg tttgtttcca gacttgtaaa aggcatccaa aacatccaaa cgacggatgg
     6181 ctttgacacg cttttgtcca gtagctgttt tcaattcttc tttgagttca gcaatttctt
     6241 tttcaagatc tacttgcttc aaaaggtctt ggatggcttc cgcacccatc ttggcaacaa
     6301 atgaaccata accatattca cgcaagcgct ctcggtattc gcgctctgtc atgatagact
     6361 tgtgctcaag tggtgtatcc ttaggatcaa tcaccacata agccgcaaag tagataactt
     6421 cctcgagggc acgagggctc atatcaaggg tcaagcccat acggcttgga atccccttga
     6481 agtaccagat gtgagataca ggagctttca attcgatatg tcccatacgc tcacgacgaa
     6541 ctttcgtacg cgttacttca accccacagc ggtcacaaac aattcctctg taacgaatgc
     6601 gtttgtactt accacaagca cattcccagt cttttgtagg accaaagatc acttcatcaa
     6661 agagtccttc acgttctggt ttcaaggtac gataattgat tgtttcaggt tttttgactt
     6721 ctccataaga ccatgaacgg actttacttg gagaagctag ggtgatttgc atacttttaa
     6781 aacgatttac atcaaccact atttcttccc tttctattct aagtgaactg cttattcttg
     6841 ttcagcagct tcttctgttg cttccgcttt tgttgctttc tcagcttctt cagcttcaaa
     6901 ggctgcttta gcctcttggg ctgctttttc gcgggctttt tcaaggtcat ctacgtggat
     6961 gacatcttcg tccattcctt catccaagtc gcgaagttcc acttcttggt catcttcgtc
     7021 taggacacgc atgtcaagac caagagattg caattctttg acaagaactc ggaaggattc
     7081 tggaacacct ggttttggaa ttggtttgcc ttttgtaata gcttcatagg ctttcaaacg
     7141 tccgttgata tcgtccgact tgtaagtcaa gatttcttga aggacatttg acgcaccgta
     7201 ggcttcaaga gcccaaacct ccatctcacc gaaacgttgt ccaccaaact gagccttacc
     7261 tccgagtggt tgttgggtaa cagttgagta tggtccgact gaacgcgcgt gcaatttatc
     7321 atcaaccatg tggtggagtt tgatcatgta catgactccg acagaaacac ggttatcaaa
     7381 cggttcacca gtacgtccat cgtaaaggat cgttttggca tcgctatcca tacctgcttc
     7441 tttaacagtt gaccaaagat cttcagaact tgctccatca aagactggtg tagcgatgtg
     7501 aataccaaga gtacgagctg ccataccaag gtgaagctcc ataacctgac cgatattcat
     7561 acgtgatggt accccaagtg ggttcaacat gatgtcgact ggagttccgt ctggaaggta
     7621 aggcatgtct tctacaggaa cgatacgaga gacaacccct ttgtttccgt gacgtccggc
     7681 cattttatct ccgaccttaa tcttacgttt ttgagcgatg taaacacgaa ccaacatgtt
     7741 aacacctgat tgcaactcat ctccatttac acgtgtaaag atcttaacat cacgaacgac
     7801 accatcggca ccgtgtggta cacgaagaga agtatcacgc acttcacgag acttgtctcc
     7861 aaagatagcg tgcaagagac gttcttcagc tgaaagatct ttctcaccct taggtgttac
     7921 tttacctaca agaatatcac cttctttaac ctcagcacca atacggataa tccccatttc
     7981 gtcaaggtct ttgagggcat cttcaccaac gtttggaatt tcgcgagtga tttcttcagg
     8041 cccaagcttt gtatcgcgcg tttctgattc gtattcttca aggtgaacag atgtgtagac
     8101 atcgtccttc accaagcgtt cgctcatgat aacggcatcc tcgaagttgt aaccttccca
     8161 agtcatgtag gcaacgattg ggttttgtcc aagcgccatt tctccatttt ccatagaagg
     8221 tccgtcagcg atgaaatcgc ctttttcaac gacatcacca acttttacga gagtgcgttg
     8281 gttgtaagca gtacctgagt ttgaacgacg gaatttttgg atgtggtaaa catccaatga
     8341 accatcttca cgacgaactt ctaccttgtc agcatctgcg taagtaactt taccatcata
     8401 ctgagcaatc acagccgcac cagaatcgtg ggctgcttgg tattccatac cagtaccaac
     8461 gtaaggtgcc tgaggattaa tcaatggcac agcctgacgt tgcatattgg ctcccatgag
     8521 ggcacggttg gagtcatcgt tttccaagaa aggaatacat gctgtcgcaa cggcaactac
     8581 ctgttttggt gaaacgtcca tgtagtcaac aatattagct ggatactctt ggttgacccc
     8641 ttggtgacgt cccatgacaa tcttctcagc aaaggttcca tcttcattca gacgagagtt
     8701 agcctgagct acagtatatt catcttcttc atcagctgtc aaccaaacaa tttcgttcgt
     8761 gacaacacct gtttcacggt caaccttacg gtatggtgtt tgaacaaaac catatttgtt
     8821 caagtgtcca taagatgaca agttattgat caaaccgatg ttaggtcctt caggtgtctc
     8881 gattggacac atacgaccat agtgagtgta gtgcacgtca cgtacttcat atccagcacg
     8941 gtcacgagtc aaaccaccag gtcctaaggc tgacaaacgg cgtttgtgag acaactcaga
     9001 aagcgggttg tgttggtcca tgaactgtga caactgtgat gaaccaaaga attctttaac
     9061 tgcagctgtt acaggacgga tattgataat ttgttgtggt gtcaagactt cattgtcctg
     9121 aacagacata cgttcacgga cattacgttc catacgagaa agtcccaaac gtacttggtt
     9181 ggcaagcaat tcaccaaccg cacggatacg acgatttcca aggtggtcga tatcatctac
     9241 acggccaagt ccttcagcca agttgaggaa gtagctcatc tcagcaagga tatctgcagg
     9301 agtcaccgta cgaaccttgt catctgggtt agcattacca atgatcgtta cgacgcgatc
     9361 tggatcagtt ggagcaataa ccttgaattt ttgaagaaca acaggctcag tcacaacggc
     9421 tgcatcgttt gggatgtaga caatcttgtt caagtcgcca tccaaatggc tttcaatgct
     9481 ttcaatcacg ctacgagtca taatcgtacc agcttctacc aagatttctc cagtttcagg
     9541 gtctaccaat ggctctgcaa tggtttggtt gagcaaacgt gttttaacat tgagtttttt
     9601 attgattttg taacgaccaa ctgctgccaa gtcataacga cgtgggtcaa agaagcgagc
     9661 tacaagcaag ctacgtgagc tttcagccgt cttaggctca cctggacgaa ggcgttcgta
     9721 aatttctttc aaggcttcgt ctgtacgaga gtccattgga ttcttgtgga tatctttttc
     9781 aacagtgttg cgaaccaatt cgctgtcacc aaagatatca aagatttcat catcacctga
     9841 gaaaccaaga gcacgaacca aggttgtaaa tggaatctta cgagtacggt cgatacgagt
     9901 gtaggtgata tcttttgagt cgctttcaag ttccaaccaa gctccacggt tagggataac
     9961 agttgaacca tagcccacct taccattttt gtctactttg tcgttaaagt aaacacctgg
    10021 tgagcggacc aactgagaaa cgataatacg ttcaccacca ttgatgatga aagtacccat
    10081 ttctgtcatg attgggaaat caccaaagaa aacttcttgg gtcttgattt cgcttgtttc
    10141 tttattgatc aaacggaagg ttacaaaaat tggtgctgag tagctagcat cgtggatacg
    10201 agcttcttct agcgtatatt ttggttcctt gatttcatat ccaacaaatt ccaactccat
    10261 tgtgtctgtg aagtttgaaa ttggcaatac atcttcaaac acttccttaa gaccgtggtc
    10321 taggaaagct ttgaatgagt cagtttgaat ttcaatcaaa tttggtaagt caagaacttc
    10381 tttgattctt gaaaaactac gacgggtacg atgtttcccg tattgaacgt catgtcctgc
    10441 caagatgatt ctcctttgta aataagttcc aagccttgtc aatcaggctt ttctaatcgt
    10501 catatggttg taaacccctt atcaccgtgt cctcttgatg aattttcaga atctttaagc
    10561 ctctgttaca aatgctcaaa atcttgaaaa aaagcacaaa aagagcagct aaatctgact
    10621 ttttcagaag atttaactgc tgtgagcctt gtctggacaa tatttcagac aaaacctacg
    10681 acaaatgatt acccatatta taccctattt agctagattt ttcaaggggt ttcagtaggt
    10741 ttttggtaaa ttttttccca tagaaaactt ggcatcacat tcgaatcacg ctatggtaca
    10801 aaaaactgaa aaaactattg actgaaaatc attttcaagg tataataata aacgttaagg
    10861 cggtatagcc aagtggtaag gcacggctct gcaaaagctt gatcgtcggt tcaaatccgt
    10921 ctaccgcctt ctataacttg atttatcagg tttcaaatga acagaaagcc caatttgaag
    10981 ggcttttttt attttccctc gaataaatac gtataacttt aaaaactttt ggagcgagtt
    11041 tgtggcagag ttctttccat ggcataattc ccttttgaaa tcaattgaaa aaagcttcgt
    11101 cacgcctggt gacaaaagcc tctagtttat tctgtttctt cttctatttc gacttctgtg
    11161 atttggactt ttaccttggt aacacgtcca ttcttcactt tatcattggt taagataaac
    11221 tgtttgtttt ggctaaccaa ttcatagctg agtttctcag tcgttggaat agttcccact
    11281 cctgtcaaat aataaccagc gatggtatca acgtcatcac tttccagttc aacatcaaag
    11341 taattattga aatcattgag ggtcatagtt ccttgaacaa tgtaggtatc ctcgccaatc
    11401 tgatgaacat ctattgctgc tttgtccgtt tcatcatcaa tttcaccaac aatttcctct
    11461 aagaggtctt ccaaagtcac caaaccagcc ataccaccgt attcatccag caagattgcc
    11521 atttgtcttt gggtatttcg cagttctttt agcaagtcat ccacaaaaat agtttcaggt
    11581 acaaaaagtg gatcttgcaa aattctcttc caaacaatat tgtcaaaacc gtccacaaag
    11641 cctgccttaa ggagactctt ggtgtgaatg attccaatta cattgtcctt atccccatca
    11701 taaaccggga tacgagaata attttgtttt aaaatacttt ggataatggc ttgactatca
    11761 tcctgaatat ccaccataaa ggcatccgtt cgaggaacca taacctctcg tgccatcagt
    11821 tcatcgagcg aaaagacacc ttgtagcatc tcaatctcat cagcatccaa tgtttcttca
    11881 ctatttgtca gcatataggc aatttcatca cgggtcatct tttcatccgc atcatcgaat
    11941 gacataggag tcaaatggct caagaaattg gtcgaagcag ctaaaagcca aacaaaagga
    12001 ctgactagtt ttccaatccc aatgataacc ggcgctgtac gaattgccaa ggcatccttt
    12061 agattaagag cgattctctt aggatataat tccccaaaaa cgatggaaat ataggtcaaa
    12121 aatgccaagg atagaaaagt tgccacggct tgtgctgttt cgccattccc aagccaagag
    12181 gcaatcacac gtcctagagt attagttaaa ctcgcccctg ataagattgt aatcagggtg
    12241 attcctacct gaatggttga taaaaagtgg ttaggatttt ctagtacctt cagcaggcgg
    12301 atgtagcgtc tgtctccttc ttccgccttt tgttcaactc gggcacgatt aagagaaacc
    12361 atggccattt ctgtggctga gaaaaaggca tttaataccg tcaagataaa caataaaaca
    12421 aattgtagca acaaattctg actacttggg tcttccatag ttcttctcct aaaatagtat
    12481 cttatccttt attatatcac aaaatttaat ccaatacata ttattttttc ttaccgtaac
    12541 ttctcaaagt aacttccatc tctctcccaa aactggaagt tagttttaga agttgctatt
    12601 ttttcttagt acctgttcta gccctgctta aactagtaaa aagaactcct acatccataa
    12661 gtgacatagg agtttaggct tatcttttac tgagttacag ttacttctcc agttcggtaa
    12721 tccagttgaa gtcccttttg aacattggga actagaacat tgaattccaa ttctccatcc
    12781 gaagacttgg cttcaatctg tgaagctgag ggaactaaat cctcgtttga agcgtagtaa
    12841 agggttacac ggtaacggac acgcttgttt tggtccaagg ctttacgcac cttgctttca
    12901 tagtagtttt gaccagtcga atactcggct tgtgcctgat ttgcccaggc tgtctgaaca
    12961 gcaatgtttt taggattgct tgttgaggca tcaaaaccat ccaaaccacc gattaaggca
    13021 tagcctaaca aatgacctct atcgactgca tgggtataag agccctttag attcttgacc
    13081 tgatgccaac ctggaggagt ccaagaagtt gaaccattcc cagtttcttt acgattcttg
    13141 tactgacgag tggccttaga caagagggca ttagctacgg ttggaacagt ttccttgccc
    13201 actgtctttg ttttattgtc agcgtagggc ttacttgaaa ccttggcatc tagatttgtt
    13261 ttattaccat tgacgataaa agcacctgag ccattccact ccagactccc ctttatttga
    13321 ctcttgactg cgtctgttaa gacactctct gccaatgctt gactaggagc ttcagacgct
    13381 tgttttttct gactaagatt ggttttggga ctattaggtg ccgacggcat ctgcttgata
    13441 taatagctcc ctgtagacaa aagcaataac actagcagtc cgattagtgt ctgtcttgtt
    13501 tttttgttca tatttctcct tatctttaga aaaaggctgg ttctccagcc taatttcctg
    13561 taaatttatg aatcaattcc tgccattttg ctggagacag gatagcccat ggatcttgac
    13621 ccttgcccaa gattccataa cctaccatta aaccgattcc tagagccaga gtacctaaaa
    13681 tcagtactat gatgactaaa agtaaacgct tgactacgta gcttgatttc ttattcatct
    13741 tcatcacttg cctcaatccg ctgaatctta gcacctagct gcgccaactt ctcatggaaa
    13801 ccgtagtaac ctctatccaa gtgaaccaat ttaccgacca cagtttctcc ctgtgctacc
    13861 aaacctgtca aaatcaaggc tgcactggca cgaaggtcag ttgaaagaac ttctgctccc
    13921 tgcaaaggct gtccaccaac aatacgagct gtatcacgga taatctcaga atgcaagccc
    13981 atgcggcgca tctcttctag gtgttggaaa cgattttcga aaactgtctc caccatggtt
    14041 gattcgcctt ttgcaactgt catcaaggct gtaaattgag cctgcatatc tgttggaaat
    14101 cctgggtggg gcaaggtttt cacatgaaca gcttttagat tttctagttg agaacgaaca
    14161 cgaattcctt cgtcttcttc aattacttca acacccattt caagtaactt ggcaatcaag
    14221 ggacggttgt gctcccagac agcgtctcga atcaagacat caccaccagt catggcagca
    14281 gctaccataa aggttcctgc ttcgatacgg tcttggacta cattgtgagt cgtaccatga
    14341 agtttctcaa caccagtaat ggttatagtc tctgtaccag cacctttgac cttggctccc
    14401 atttcattaa ggagaatggc taagtcaaca atctcaggct cacgcgcagc attctcaatc
    14461 actgtcaccc catcagccag agtcgctgcc atcatcaagt tctgcgttgc accaacactt
    14521 ggaaagtcca tatagatatg agcgccatgc aagcgttctg ccttggcttc gatgtaacca
    14581 gctgtctgac taatcttaac ccccatagct tccagacctt tcaaatgaag atcaatagga
    14641 cggctaccaa tcgtacaacc acctggcatg gataccttgg catgacccac acgggcaagg
    14701 attggcccta atacaacgat ggaggcgcgc atcttgctga catacttgta aggggcttcc
    14761 tcagtgatgt cgccagtagc atccaccttg acaagatgag cttcctcatc aaagtcaacc
    14821 ttggcattca aaccaccaac tacctgattc ataataaaga catccgacaa aatcggaaca
    14881 ttctgcaaga cggtctttcc ttcacttgct agaatagtcg ctgccaacaa gggtaagact
    14941 gcattttttg ctccctcgat cgtcacgctt cctaccagac gattatcgcc accttgaacc
    15001 acaattttat ccatactcgt ttcctttact cttgatttca taataatcct ctaagcgctt
    15061 cttgccacaa atgatacagg ctaataaaga aagaactcag gatgtagcct atgacaatgc
    15121 taaaaaaggc tactaataaa cgaacttttc ttgtattatc atctgtcact tttaaaacct
    15181 tctcccatct aacaagatcc tttaaaaggt aaaaactcac ataaataaag aacatatgac
    15241 tacttagagt gaataataat tgaaccattt gactattata ccatcttctc tgtttgtgcg
    15301 ctagtttgga aacttcactc aaaaattagg gattgttttt caagtaatca atcgcatctt
    15361 gtagggtttt aacgggcacg attttcatat ccgtcttgat tgttttagcc gcttctaggg
    15421 ctgtttggta gttgtttttc gcgtccggat gcgccttttg ttcttcttcg ctaacagggt
    15481 tatcaggagc aaagaaaata gcagcacctg ccctagccga agctacaacc ttcttatcaa
    15541 tacctccaat gtctcccaca ttaccatcgc ggtcaatggt acctgtaccg gcaacaatac
    15601 gaccattacg aagatctggg tgagctattt gagtatagat agctagacta aacatgagac
    15661 cagcacttgg accgccaata ccagctgttg aaaagctaat tgggacattg ctgattacct
    15721 ctgtacggtc aatcaagccg attccaattc catttttgcc attttccaag gtgatgattt
    15781 ttccttctgc agacttggtt tgcccatcct cttcataggt gaccttgacg gaatccccta
    15841 atttttgaga actgacgtaa tcaatcaagt ctttggaact atcaaaggtc tgatcattga
    15901 ctgctgtgac tgtatcagag atattgagaa tctctttaaa ggttgaatta tccgtcacat
    15961 tcaaaacata aactccaaag tacttgagtt cgatatcctt accagctgtt tttagtcctt
    16021 gatacttggc catattttgc gatgtttgca tgtagaattg attgattcgc ataaattcaa
    16081 catcggaaga accacctgta gtctcctgag cactacgaat atctgtaaaa ggtgtcaacc
    16141 aagcataaat catatgagct aaagtggcat gttgaacacc aaccgtaacg aattgatagg
    16201 caccagcttc cttatcttct gtgtcattta ctttaaggac ttggcgaata tcttccgaac
    16261 cacctggaac ctctatataa taaggcaagg gcactacaaa tgccaagaaa gtcacaatca
    16321 aggccgcaat gacgtataag ggccatctaa tctttttttt catttcttat ttcctccaaa
    16381 atactctcgg gaacatggca ggcaatatcc tgaccaaact tcaaaagctc tcgaacacct
    16441 gatgaactga tatagagatg ttcaggccgg ctatgtaaat aaatagtctc tatatcagaa
    16501 gacagctgat gattgtagta atcaaaacta gcttcatatt gcaaatccgc cgcattcctc
    16561 aacccccgta ctaggaaagt agcccccagt ctttttgcaa catcgaccac caattcatca
    16621 tgagaagcca cgacttcaac attttccaaa tgtcccaaag ccttttctag cccccgttta
    16681 cgattttcaa gagggagaaa tccttgtttg tggggattaa aaaaaatacc cacataaagc
    16741 ttatcaaaaa gtctgctcgc ccgttcaatg atatccagat gcccatttgt catcggatca
    16801 aatgagcctg tgaataagcc aatcttatct gacatagact gtcaccttac taattccata
    16861 aatcttttcc ttccagatac ccagacaggc aatctcttct ggaagttcaa cggccttatc
    16921 cgtctcgcac acaaccataa catcttcaga aaaaagctct ctcttagcca ttttttcaat
    16981 atctgctacg atttgttcct tggcataggg agggtctaag aaaacgaggt caaattcccc
    17041 agatacctgt tccaatgccc tctctgcatc catcttgagg agttgaaatt ttccaacttc
    17101 cttggtcatc tggatatttt cagccacgat ggtctgagcc ttacggtctc gctccaccaa
    17161 aacagcactg gacatgccac gcgatactgc ttcgatagat aaaccaccac tacctgcata
    17221 aaggtccaag actcgtcccc cttcaaagta gggaccaatc atgttaaaaa tggctcccct
    17281 aaccttatcc gaagtaggtc ttgttgtctt gccttctagt gtcttgaggg gacgtccccc
    17341 atagattcct gatacgattt tcatactgtt tattatacca aattatagac aaaaagagaa
    17401 agaaaaccga accttgcggt tcgattctct acaaaatatt ttcgtaagta tcgcggactt
    17461 cttgaggcca aacacttgtt tgcacttctc cgatgtgtct cttgcgaagt aggaacatgg
    17521 ccatacgaga ttgtccaatt cctccaccga ttgtcaatgg gaataggcca ttcaacaaag
    17581 acttgtgcca ttccaattcc aagcggtctt catcacctgt aatttccacc tgacgtctaa
    17641 gagtttcttc atctacacga attcccatag aagacaactc aaaggctcca cctaaagact
    17701 cattccagac aagaatatca ccatttagac ccttgtagcc attctcagac tcgcttgtcc
    17761 agtcatcata gtctggtgca cgtccatcgt gcggtttacc atctggcaac tcgccaccga
    17821 taccaatcaa aaagacggct ccaaattctt tacaaatcgc attttcacgt tctttaggtg
    17881 tcaagtctgg gtagcgttct accaattctt ctgtatggat aaaggtgatt tgttttggca
    17941 agatagactc gatgtcatag cgggcttcaa cagctagctc agtcaggcga atagccttat
    18001 aaatcttctc aactgtttct tttagataaa cgatgttacg tttaccattt gggataacct
    18061 tctcccagtc ccactggtca acataaacag agtgggttgc atccaaggaa tcctcatctg
    18121 gacgaagggc tttcatgtgg acaaagagac cctctccttc accaaagcca aaacgagcca
    18181 aggtgtggcg tttccattta gcaagtgagt gcaccacttc ataagtagca tcagggattt
    18241 ggagaacctt gaccgatact ggattttcca caccagacag gttgtcctgc attccgtcac
    18301 cgaccttact caagatagga ccttgaactt cgacaacttc tagcttatct ttcaaatact
    18361 gggtaaaagt atttttgaca aaggaaattt cttcttgttg atggataaaa cttttcttca
    18421 tagacaactc ctcaaaaatt taataaacgt attatacacc tatttccaag aaaagaaaag
    18481 agaaaattta aaaaaatcag tgtcactcaa aacactgatt tcatagacct tttagtcatt
    18541 gcgaccaaag atacgtagaa tacttaggaa aagattgata aaatcaagat agatgctgag
    18601 agccattgac accacccagc ctgtcgctac acgaccttgt gattgttcat aggcgaggcg
    18661 aatcttttgg ttatcccaag caatcaaccc tgaaaaaacc aaaaccatgg ctacgctaat
    18721 catataatca aagaagccac tagccaagaa aatattgact accatagcaa tcagtaaacc
    18781 aatgagagct gccatcattg ctcgaccaat cccactcaag tctttcttgg taaaaatacc
    18841 aactgccgcc atgacaaaga agagaagggc gctcgataca aaggcagata aaactgtacc
    18901 cggagtatag aaagccacca caaaactaag ggtaaagccg tttaaaacgg agtaaagtaa
    18961 aaatactgga agagcagctg gactatttct tgaagccatg ctactggcaa cgaaaaccaa
    19021 agccaactcc gcaaaagtag caatggtcaa ccagagacgc ccctgcatca acaagtaaac
    19081 caactgagac tgaaagaccg tcaacataag gcctgatacc aaagcagata gtccgattcc
    19141 cagaccaaca aaggcataaa ccttagcgta aaattgattg agacctgcac ggtcatgtat
    19201 aatagtgtga ttcatccttt tctccttttc tgtgaacatc tttccttgat tataacaaaa
    19261 aaagttaaaa ttagcttaaa tggaagatta aaataccggt tgcaactgca acattcaaac
    19321 tctcggcctg ccccttcatg ctaatatgga ccaactggtc tgcactttca gccataaggg
    19381 gactaattcc ttgaccctca tttcccataa ctagtacaaa attttctata gaaggcagtt
    19441 ctctgtaatc aacagaatct ttagataggg ttgttgctag cactgggata gctacctttt
    19501 tagtttcgtc aagaagcgct tgactagtca tccggtaaat gggcagatgg aaatgactac
    19561 cttgcatgga acgtagggtt ttgagactgt agatgtctgc cgacttatct gaaacaatca
    19621 ctcctgtaaa acctgctgca tccgcagttc gaatgatagt tcctacatta ccaggatctt
    19681 gcacatcttc caaaaacaag aacttgccct gactcaaatc agcttgtcct acttcttctt
    19741 tttgaaccac ggcaacaatt ccctgtggag tctgagaatc cgccaaatct agcaaaatat
    19801 cctctgaaat ccagacagtt tgcggaaaag cagctaactg atttcgataa ttttctaggg
    19861 caaagatttt ctcaatcgtc actccagctt ggacagcttc ttcaaacaag tgccagcctt
    19921 caatcaaata ggcagacttg cggtattttt tttggtgtaa tttcttggca ttttttacca
    19981 cagaattggc ttttgaggtt ataatagtca tagaaatatt ataacacaat caaaggggtt
    20041 tggtatgcaa aaggttagaa tgattgccca aggtagggtg cagggagtcg gctttcgttg
    20101 gggtgtttat agcctggccc ttgaaatcgg tggcatcaca ggtcgggtat ggaataacga
    20161 cgatggcaca gtggaaatct tagcccaagc agactcatct gctatcatgg caaaatttat
    20221 ccaagaaatc cgaaaaggac cgacaccttt ttcaaaagta agctacttag atgtcaaact
    20281 aagcaacttt cctccctact ctgactttaa aatcgcaaat taggtctcca gaactattgt
    20341 atattttgta aaaaaacagt agaatagaaa ggtataattt ttaaagaagg aataaaaaca
    20401 gtgaaatcta ttaaacgttt tgcattctcg gctatgggag tggctatgtt gcttgtcttg
    20461 actggctgtg tcaatgtcga taaaaccaca ggtcagccaa caggatttat ttggaatacg
    20521 atcggagcgc ctatggctga agccatcaag tacttcgcta ctgataaagg tctaggcttt
    20581 ggtgtcgcta tcattatcgt aaccattatc gtacgcttga ttatcttacc acttggtatc
    20641 taccaatcat ggaaggcaac gcttcactct gaaaagatga acgctctcaa gcatgtcctt
    20701 gagccacacc aaacacgtct caaagaagca actactcaag aagaaaaact cgaagcccaa
    20761 caagccctct ttgccgctca aaaagagcac ggcatcagca tgtttggtgg tgtaggatgt
    20821 ttccctatcc tccttcaaat gcctttcttc tctgctatct actttgctgc ccaacatact
    20881 gaaggggttg ctcaagcaag ctacctaggc attcctctag gttctccaag tatgattttg
    20941 gttgcctgtg ctggtgtcct ttactatctt caatcgctcc tttcacttca cggagtagaa
    21001 gatgaaatgc aaagagaaca aatcaagaaa atgatttaca tgagcccact catgatcgtc
    21061 gtcttctccc tcttctcacc agctagtgtc acactttact gggttgtcgg tggtttcatg
    21121 atgattctcc aacagtttat cgtcaactat atcgttcgtc caaaacttcg caaaaaagtc
    21181 cgtgaagaac tagccaagaa cccaccaaaa gcaagtgctt tctctaaacc aagtggacga
    21241 aaagacgtta cccctgaaca accaactgct atcacaagca agaaaaaaca caaaaatcgc
    21301 aacgctggaa aacaacgttc gagataagaa gaaaaaggct tagctgattt gctaagtctt
    21361 tttgcaaatc aaaagcccga tgtttccatc aggtttcttt tctatccatg tacacgtttc
    21421 atataatctt gataactttc ggtatccatg agttttttag cgttcttgac gcgatctgct
    21481 gttggtggtt tgactccttc gagggaatat ggaattccaa gttcacgcca cttgaactca
    21541 cccatggtgt gataaggtag aatttcaaac ttatcaacat ttttgagggt cttgacgaac
    21601 ttaccaagtt caatcaagtc atcatctctg tctgtcaatc ctggaactag cacgtggcga
    21661 atccagacag gttttccaat atctgataga tactgggcac aagccaagat atttttatta
    21721 gtttggctag tgacaatctt gtgctgttct tcgttgattt ccttgatatc caaaagaacc
    21781 aagtcagtga cagccatgag tttgtcaaac ttctcaaggt aacgtggttt attacggaaa
    21841 ggaagagcac aggtatccaa ggtacagtgg attccttgtt ccttagcctt ggtgaagaga
    21901 gcaatcagga aatcaatctg caagagagct tctcctccac tgactgtaat cccaccctta
    21961 tttccccaga aaccacggta gcgcaaggcc tctgtcaaga catcatctac cgtccgttca
    22021 cgtgacttat tggactccat agcccaagtg tctgggttgt ggcaatactg gcaacgcatg
    22081 tgacagccct gcaaaaagac aataaagcga ataccaggcc catctactga cccaaagctt
    22141 tctgtcgaat gcaccattcc tgtcacttgt ccataatcaa ttgtttcttc agacatatcg
    22201 ttaccttcct tgaaaacgtt ttatagtttt attatatcac gacttgaagg aaaaacaaga
    22261 tttttgccta ttttttagaa agcaatctgt ttttcaattt cattttcaat ctattatcag
    22321 attattcttc aaaatcgaag ccatataagg ttgcaaaata gtcctcaggg cgctctgcac
    22381 ggcggatttg acgggcttta ccttcttcgg tatagaggat ttccgcagaa cgtaatttgg
    22441 cattatactg gtagcccatt gaaaatccgt gggcacctgt atcatgaatt accagcaaat
    22501 caccgatttc tgtatgaggc agttcgcgat tgactgcaaa tttatcattg ttttcacaga
    22561 gtgacccaac cacatctacc gcttcagctg gtccatctgg atgggtcacg ttgctaatat
    22621 gatggtaagc tccgtacata gctggacgca tgaggttgac tgctgaggca tccacaccta
    22681 gataggtacg gtaggtttcc ttcttatgag tcactcttgt gactagagca ccgtgaggtg
    22741 ccagcataaa acgacccaat tcggtgaaaa tcttgacctg accaagacct gctgacgtaa
    22801 gaacttcttc atacacctta cgaactccct caccaatcaa ggcgatatcg ttcggctcct
    22861 ggtctggacg ataattaaca ccaataccgc cagaaagatt gataaagtct agcgaaatgc
    22921 ccaacttttc cttgatttca acagccagtt caaagagctg acgagccaac tctggataat
    22981 agagatgggt cacggtattg gacgctagga aggagtgaat cccaaaagtc ttggctcctt
    23041 tttccttcaa gatagcaaag gcttcaaaga gctggtcctt ggtcatgcca aacttagcct
    23101 ccccaggatt gtccataatg tctgtcccca gttcaaaaac gcctccagga ttataacgac
    23161 aagagatgat ttctggaatg cctgctactc tctccagatg ttcaatatct tcaaaggcat
    23221 ccaagttaat ggtcgcaccc aattcacgtg cataggcgta ttccttgtct ggcgtgttgt
    23281 tggaagagaa cataatctca gaacccagaa agtccagttt atggctcatc aaaagctcta
    23341 cataactaga gcagtccaca ccacaacctt cttcttggag aattttcaaa atagctggag
    23401 ttggagtagc cttaactgca aaatattcct taaagccctt gttccacgaa aaagcttggt
    23461 taacggctct tgccttctca cgaatcccct tctcatcata caagtgaaag ggagtcggga
    23521 actcggcaac aatcgcttct aactcttctc tattgataaa tggtgttttc ataagcttcc
    23581 ttctattctg tttgcaaaga aaggctggga caatcgttcc aacctttagt tctatctctt
    23641 atttatcttc gtcaaagata ttgccaacct caacatcgat ggcttggttc ttgacatcaa
    23701 tattggttac cttcaagagt gacttgtagt caaactgctt ttcacgttct tcttgggcat
    23761 tgtccgcaaa gaccgctaca cctgccagtt ctgagtcgaa ctcgcgcaag agactaatca
    23821 taccattgac tgttccgcca cctttcaaga agtcatccac aatcaagaca cggctgcctg
    23881 ccttaagact acgttttgaa aggaacattt tctcgatacg gtcaccactt gaacctgaaa
    23941 catagttgac gctaacagtt gaaccttcgg taattttcag gtcacggcgc acaatgacaa
    24001 aagagacatt gaggacattg gcaactgcat ttgcaagtgg cacaccctta gttgctacgg
    24061 tcataaccgc gtcaattttt tggtccataa agcttttggc aataatacga ccaatatttt
    24121 tcaagatggc tggtgtgcta agcaaatcag acagatagat ataaccacct ggcaagatac
    24181 ggtcactttc tgacaacttg gtacgcaagt cttcaaccat ttccttagca tcctgactcg
    24241 aaatagacgg tgtgaagatg acacctccgc cagccccagt cactgtctgg atatgaccga
    24301 tttcaatttc ctcaaaggcg cgtttgataa tgacgatatc ttctgagatg gatgatttag
    24361 cagactcata cttttcagca aaagtattga gactagttag tttataagga ttattaatca
    24421 aatagttgga aatgacaacc atccgatcac ttcttcttaa tttcatttct atttcctatt
    24481 ccatttttat tcaaaaaatc aaaaagcaaa ctagaaagct ggtcgctggt ggttcaaaac
    24541 actgttttga gattgtcaat agaactgaca aaccctgtaa tatacctgca tatatacata
    24601 cgacaaggcg atactaccct agtttgaaga gattttcgaa gagtattcat ttttgtcttt
    24661 tacttattat accatattca cataaaaaaa cgaacattct tatcctaaaa aatgctcatt
    24721 tttcttaaat tattaatcta aatctggttt atagaaggaa cgattatcca tagcgaagat
    24781 tttattggac atctctcctt tatccaccaa agccagagct gttgacatca tcatcatgct
    24841 tgcatccaga ttgtcaatca tatggataat ctctgcctcc ataatacgtg gacggactgg
    24901 acttccatat tcaagcaagc cgtggtgact gaggatgaca tgacgaagca aaacgacttc
    24961 ttccttggta tcatcgatgc cgagttccat aactgtcttg gtaatttcgc tatcaatcag
    25021 agcgatatgt ccaagaagat tgcctctgac cgtgtactcg gtctggtctg gtcccgtcaa
    25081 ctcgatgacc ttagccaagt cgtgcaacat aatccccgca tagagcagac tcttattgag
    25141 ctgaggataa acttcgctaa tagcgtctgc caaacgtacc atggtcgccg tatgataagc
    25201 caaccccgtt tcaaaggcat ggtggttggt cttggcagct ggataggagt agaattcctt
    25261 atcatactta gtgtagagat ttcggacaat ccgttgccag acaggatttt caattttgaa
    25321 aatcatttgc gacatgtagt cacgaatttc cttgacatca actggtgact tgaccttgaa
    25381 atcggctggg tcattgggtt caccagcttg aggcaggcgg agagtaattt gattgacttg
    25441 aggggtattg ttataaactt ctcggcgtcc tttcatgtgg acaaccttac ctgcggtaaa
    25501 ggcctcaatg ttatgaggtt gggcatccca gagcttccca tcaatctcgc cactatcatc
    25561 ttggaaggta aaggctaggt agtttttccc agctcgagtt tgcctcaggt cagctgattt
    25621 gattaggtaa aagccttcaa atagctcatc ttttttcatg tgactaatct tcatattctt
    25681 cctcattttc ttgaaaatgg agtagatcaa gcgcaggctc accttctgac aactcaatgt
    25741 gacggagcgt ccgctcgata gctatggtac gacggtttaa taattcatca atattgccag
    25801 aggcatgttg gagatgtttt tgtgccttga ccagaatacc accaaacttg ccaaactcgg
    25861 tcttgacact ggcaagagtc ttgctgatat ggtcggcact cttttggata ttaagggtct
    25921 tgaaaccaac tgatagggag ttaagaaggg ctgatagggt acttggtcct gcaacgataa
    25981 tctgttcttc ccgtctcaaa tcatcaaaga agaccggatt gcgcacaatc tctgaataaa
    26041 gaccttctgt cggaacaaac aagattccaa aattggttgt ccgaggtggt gctatgtact
    26101 tgttcctaat atccctagca aaacgcttga cacttgctag gagcgactta cgacagcgtt
    26161 cgatttcatc cttgtcacct acttcatagg cttcttctaa gcggtaataa tctgccagtg
    26221 gaaacttaga gtcaattggt aggtagacgt attcttggtc gccttgtccg ggtaacttga
    26281 tggcatactc cactcgttca ctagagtttt caaccgttgc gtattctcgt tcgtactggg
    26341 caggtgtcat gatgtcttca ataatttgcc ccagttgcaa ttctcccaga atccctcgcg
    26401 tcttggttcc agagagaacc ttgttaagag ctccgacatc acgggcaact gtctgcattt
    26461 ctccaagacc acgattgaca gactccagtt gtttagaaac tgtctcaaag gaagcctgta
    26521 agcgtgtctg caaggtcttt tctagttttt cctcgaccgt ctggcgcatt tgttccaaac
    26581 gttgctcatt tgattcctgc aaggcttgga gacgttggtc tgtcttgtct ctagtttgga
    26641 ggagattatc tgtcatttct tgacggactt gggtcaggcc ttggtgcaat tcaagccgca
    26701 cttcttgcaa acggtcgctg acagccactt ccaaatcttt ttggtctaac tggctggctt
    26761 gtctggcttg gtcaaagcgg taatccaact ggtctgacaa atgatctgcc tgatcctcca
    26821 agctcttact taagtgtttc tcctgcctat cctgcctttg ccaaatcaga aagagaccag
    26881 ctaggttggc aattaataat agaataagta aactttccat cctacctcct gtccttgcta
    26941 tgcagtacga ccacataacc atctgggcaa gttaccgaca cttccctatc tatatattcg
    27001 ttagaagcgt acactttttt aaagaaaaaa ttctcctctg tcaactcata cttagctcca
    27061 agaatagtca gctggctatc ccgaactggc ataaaggcta gatagtcgta gtctgaacga
    27121 ggttctagct gactggtccc ttctgaacaa taggcaatca aattttgccc atcctcaatt
    27181 tctatttgac gcatataggg tgccaactta ggattgctag gtagaaagac attggccaac
    27241 atatggtcaa tacggccacc caaggcaccg aaaatagtga cctgagcctg aggattttgt
    27301 tcaaagatgg ttaagagagc caattccaga tctgtatcat ccttttctgg tcgtgcttgg
    27361 acaaaatact gggcacgttt ttgaatcacc tgtcgctctt cttccgtcac agaatcaaaa
    27421 tctccgactg ctagagcaag aggtaagtct tcttccaaga cccagagcga gcctcgatcc
    27481 accccaacaa aagcatcaaa atctgtccga taatgaccgc ggtttccgcc tgcaaaaact
    27541 gcaaccctag tccagttgtt ttctgagagt ttgtactcgc tcattgactt ctcccttaaa
    27601 gacataggaa cctgctacaa aaacagtcgc accagcttct ttggcttgag caatagtttg
    27661 gtcatcaatc ccaccatcca cttcgatttc aaagttcaaa cctttttcct cacgaagagc
    27721 aaccaactca cgaaccttat ccatggtttc tggcagaaag gcttgcccac caaaacctgg
    27781 attaaccgtc atgactaaaa cttggtcaac tagatgaagg acgtgcttga tggcttcaac
    27841 tggtgtgcca ggattgataa cgactgaagg cttaactccg agtgaacgaa ttttttggag
    27901 ggcgccatga atatgaggtg ttgcttctac atggatactg atgatgtctg cacctgcacg
    27961 cgcaaaatct tccagatgat gctcagggtt tgacaccatc aagtggcaat cgaaaaccat
    28021 cttactatga ggacgaaggc tctcgaccac acctgcacca aaactgattt gcggtacaaa
    28081 atgactgtcc atgatatcga tatgggcata ttctgcccca gttgcttcta gacgtttgat
    28141 ttcacgttca aagttggcat aatctgctgc cagaattgac ggagcaatct tgtattgaga
    28201 cataggtttc tccttatttt ggaatttttt tgctgacttt tttataggtt tctctacgat
    28261 tttcaatttc actaaggaat tgcaggtaat tgtcaaaacg gaaggttgca ataacaccct
    28321 cttcaacagc tggtttgacg gcacaagacg gctcatgggt atgggtacaa gtacggaact
    28381 tacaatctcg gctaacagta gcaatctctg ggaaagcctg attgaggtct tcagcccttg
    28441 atacttcata gtccaaggat gaaaatcctg gtgtatctgc gattttaccc ccgttgagat
    28501 tgtaaaaact aacagctcga gtggtatggc gaccgcgacc tagactgtct gaaatttctc
    28561 ccgtttcaag attgaggtct ggtgcgattt tattgagaag agttgacttc ccaacacctg
    28621 tctgccccat aaagaccgta accttgcctg ttaacaaaga caggagttcc tctttactgg
    28681 tcacaaagtc atagccgatg tcaccatagg tctgctggta aaaatccagt tctcccctat
    28741 cttccaacaa atccattttg gaaatataga caatgggatg gatgcccttg tgctccaaaa
    28801 gaaccaagaa acgatccagc aaattgctgt taaaatcagg ttccttgacg gacatgatta
    28861 ctacagcttg atcgatattg acaataggcg gacgaaccag actgtttttc cgttcgtgaa
    28921 ttttgaggat atagccttct gaattttcct cggcagagaa atctacccag tccccaacat
    28981 aaggggtatg gcctttttta cggaaattcc cacgcgcgcg tgtttgataa acctggccat
    29041 cactctccac atagtagaaa cctgccaagg ctttaatgat ttgtccctgc atcttagagt
    29101 ccttgccctt taagtgcgtc tgctagacca gcaaattctg ccaagctgag agcttcccca
    29161 cgcacacttg gtgacaagcc tgcctggtcc aaagccttgg tcagcttgtc cttgacctct
    29221 tcagtcttac caaagtaacc tgtcaagtta ttccacaagg tcttgcggcg atgggtaaaa
    29281 ctagccttgg aaaccttaaa gaaaaagttc tcatcttcta ctgctacggc tggctctgga
    29341 cgacgcacca ttttcaagat ggctgaatcc acatttggcg ctggcacaaa gaccgtacga
    29401 ggcacgataa aggcaacctt ggctgtcatg taatactgca cggcgataga caagctaccg
    29461 taagccttgg tgttaggctg ggctgaaatg cggtccgcta cttctttctg catcatgacc
    29521 acaaactcac taaaaggaat gccactctca atcaagtgca tgagaatagg cgtcgtgatg
    29581 tagtaaggca aattagccac taccttgatt ggcaggttag gatttttaaa attttggata
    29641 tgttgcgcca aatcaacctt gagaatatct tcgttaacta cggtcacatt atcaaaatca
    29701 cgcagggtat ctgccaaaat tggcaccaaa cggtggtcaa tctcaaaagc catgacttgg
    29761 gctgcacgct cagccaaaaa ttctgtcaag gcaccaatac ctggcccgat ttcgatgaca
    29821 ttgacctgat catcaatttc agccgtatcc acaatttttt gaaggatatt ggtatccgtc
    29881 aggaaatttt gcccgaagga ctttttaaag gtaaaaccgt gacgctccag cactgccttg
    29941 gtcacgctat aatctgcaat tctcatctat tctcttttct attatctgtt actactattg
    30001 taacacattc cccttgtatt ttgggaatga aaatcatacc aaaaaactat ctttcctcat
    30061 gaagaaagat agtcttggaa atctttaaaa gtggccttag cagttctctc gaaacatgcg
    30121 ttcaaaaaaa tcagcttctt gataacttcg attttcgggt agggtcaaat ccacatcctc
    30181 ctccacatca tccactgtca gttcaaccgt ctgaggtaaa tttttcaaac gataatgctt
    30241 ttgcctttgc ccttcaatac tagcaaaatc aagcaaatag ccatgcactc ggatttgaac
    30301 caaaaggaga tgattggtca tattttccat gatgaggata tcttctaaac cacctctggc
    30361 actaggataa tcagataccc tgcgaatacg gattttttca cacatgagca agtttcctca
    30421 tatcaatgat ttcatcagcc ttattccaga ctaggggatt atgggtcgca atgatgataa
    30481 ttcgattttc atctttcaaa tccaccaaga gattcataac ctcctctgaa ttttcaggat
    30541 caagagctgc tgttggttca tctgccaaaa tcaagggtgg atttttcaaa atagtcttag
    30601 caagggcaac tcgttgggcc tctcccccag ataaagtata gattttttgt tctaaatcca
    30661 aataccctag attaactttt tctaaagccc ccacttgcct ttccaaacgt tctacttttg
    30721 agattttctg accaacaaaa cccaaatcca aattttcttt gattgattgg ttttctaaga
    30781 ggccgaaatt ttgaaagaga tagcccatct ggtctcgaaa atactcacga gtgggaatgg
    30841 tttttaaatc tttcccctga tagagaaccc ttccaccatc tatcttttct agctttccca
    30901 agatattcag cagcgtcgtc tttccacttc cactctttcc gattaaggca taaaccttgc
    30961 ccttctccag cttgagattc aaaccagaga aaatcgcgcg gtcattaaat ttcttttcca
    31021 atccttgaat atcaatcatc ttattttcct ttcaatactg ccatgctacc agcttcttct
    31081 tttttgtcct gccttactaa aatcaagagg gcgttaagcg taaacaaagc tacaactaga
    31141 gcgctaatca aaccatctct tgtcaaaata ctagatagga ctaggccaag caagagaact
    31201 cctccttgcg ccagtaaata cttgccatga agctcataga ttgtcatacc agcaagacgt
    31261 ttaatcataa gatcccgtct gaactgctca aaatagagaa gattcatgga atcaaataac
    31321 aagatagccg tagacagggt caaaatcgtc ccaataagga gagagtaaaa ctccacccgt
    31381 ttgtcattta gtaccttggc aaaaaatagc tggcttttta ccaagtaaga aaccttgtga
    31441 tacagacctt gctcttttag agctgtgatg ctgtctccat atcgatctag tttcaaggca
    31501 ctattagccg tagttcccca caacatccct gcaacaggtc ttgctccagt cgcttgcggc
    31561 gttagaacta caataatcgg atcttttagg tactggcgtg ttgtcttgta cccatcatta
    31621 tagaggaaac gttcctgtcc tgtttctgta aaagctagcc ttacctgtgg gaggtagtgt
    31681 ttctgactcg tcacttctac actttcagat gaaaagtttt gcaggtaatc tgtaaacaat
    31741 ccttggtagt agacagactg ctctcgcaag ctctcaggca agatcagccc aaactctccc
    31801 tcagacaagt tttgcatctt gtccataaac tctgaagaaa cggtaatctt ttcttctatc
    31861 agatagcgtg gtgagacata gataacattc cctgacggtg tgtagtcact gagacgattg
    31921 ccatctaggt ccacttctgc tccatctgag aaattgtcaa cattgctcat aatataaaaa
    31981 gagtctgtat tggctaaccg ttcttcagta aatgtctgcc actcacgatt atccttacgc
    32041 gttccttctt catcggcaaa tgcactagac caaccaaagg atagacggta acggtctgag
    32101 gactggctcc atttattgct agctctctcc atttcctgca tttcacggta gtggggtagg
    32161 agagctgtcg cactcgatcc gaccaccaat acagctaaga gttgccccac catcatcaat
    32221 gtcatcatac gtttgagagg gagtttccct ttcaatagat ccaccagact attttcctgt
    32281 aaaccaagta gatagacgac acttagtaag gtagaaatcc ctgccaaggt caatccataa
    32341 agtagaagag caatgatgac cagttgcacc gttgccataa acaaggcacc ttgataccag
    32401 agaatcccca atcccaaaag actggatacc agcactgagc agataagctg tctcacatct
    32461 tctaacactg gtctgagagc aactccaaac aagctctcac cagctattaa gcgaatccct
    32521 gcctgacgaa gggatttgat ccgataaatc agggtcagac tcataaaggt cagcagaaaa
    32581 atagctaaac tcagtagcac cataggggtg gccgtcaata gtaagactat cgaaaatgga
    32641 tcttctccat tcgaaacaaa gccttgataa ccaagctctt tcaaggtggt ctgtaagctc
    32701 actccatcca aacttcctga cacaatcaag taagacccta ataaatcact cgtttctgca
    32761 ctctccttgg aggaaatggt cagaccttct ggaagctttc cctcaccata agtggcatag
    32821 gtaaagcgtg tttctccagc ttcatttggc tcaacgattc gacgagcaat gagactattg
    32881 tgctctaccg ccaagtgact caaagcctct gtcacctctt cccgactagc tactggttga
    32941 tttatcatca ttcctgtcac atcaacagaa ggatagttac caaataaaat ctcttctcga
    33001 tggtcaacgg aagttatcac catataaagc gataccaata ccattgaaat caaaataaat
    33061 aaacgtttca tcatttcctc caaaaagtta cttggtcact tagtgagaag gtgaccaagt
    33121 aaagctaaac aatagggcta taccattcta aacattatag tagaatgcaa ctttctctcc
    33181 ccaagaagta cgaatccatg cacgagaagt ccctccagca ccagcatatc ccttgtcaga
    33241 tgaaccagtt tttctgctaa caactgttga actatggtat tttgaagggt gatagtagtt
    33301 tgagtaagca ccaccgttac ctgaaccata agtccaaaca ccaccttctg ggtattgaac
    33361 ggctgcagag gcaactgcta cacttccacc taatgttgct gcaatgctcg ctacaagagc
    33421 taatttttta actgtttgtt tcatattgat actcctctat ttttgattac aagattgttg
    33481 agttacttgc tcctctttcc attatcaacg ttgatactat tatattacca aatgatattc
    33541 taaaattatt tgtttttctc taaattgtca aaatagcacc ctgaaatgaa cttttatagt
    33601 cattatactc catctttatc acactatcaa ggacatcaat gtccttaaaa tctaactatt
    33661 tcatattatt tttagcttat atctaaaaaa acagttctaa agacgaactt tagaactgtt
    33721 ttgatacgag gacaaggaca tttttgtcac tgattcacta tgtttataaa tattttttga
    33781 catcctcttg ccattcctct gtcaatttct ttactagaaa tccatctcca attagcttcg
    33841 caaatgtttt agaagactga taaaatgatt cagctaaact aaaatctctt tctactctta
    33901 atgcttgctt ccacaaaaac ataaatacaa gaggtttctt ttgataatct tgagttttat
    33961 ccataatttc ttgtaaacac gatagataat cattaaaaca atcagttact cctacctttt
    34021 caagacaaca cattcctgaa ataagagtat ctctaattat aaagtaattc tctatttgag
    34081 ttaccttctt ctgttccaaa agaaagagca ttagcttttc gatttgattt ataaatttgg
    34141 caagatcttt tctaatgagc atttgataaa aaaacaaacg aatcaacaac atatcattta
    34201 ttgaataagc ttctttttct ataatctgag gcatatgttc ctcaagtatt aactctgcat
    34261 attcaggcct tctagttcta gaagtatcca aacacgcctg caatacttca catgctaatt
    34321 gttcttcttt aggaagatta tcaaagtatg ttttataaat ctcttccaaa cacgcttcct
    34381 tcttatcgta ctcctcttct ttaccataga ttggttcacg taaaatctgg tatttcaatt
    34441 ctaaataagc agaaggaaga gctgaaaaat ccggcataag gctataaact ggaactccta
    34501 gccggcgagc aatataggct aacttgggca aactcggttg cgaagctcca ctttcaattc
    34561 gcgacagttg acgaactgtc agttcttgct catctccaca aaaatctgga cgactaattc
    34621 ctttttctat ccgaatctct ttaatttttt gaccaattaa catcagacgc ctcctcttct
    34681 acctgcttcg aaaaatactg tgaaacttaa aaaattgtaa cggtcacatc tttcctactc
    34741 ataagatttc atagcttctt ccacttctgc caaagtaacc ccaaacaact ctaggcgttt
    34801 gaggagttgc ttgccgttag aatagccgat tcggagagtc tctccgagat attctctacg
    34861 cttacggctg tctgcccctg ctagaaaacc aaggcgaatc aaatcgctac gactaatgtc
    34921 aaactgactc tcatgttcaa attgttctgt cacctgagct agagccgttt tcaggtcttc
    34981 atagctggca tgctcaattc ccagagaacg ccccttggtc ttggacttgg gaacagcttc
    35041 atctcgcttg agaaaggcat gctgaactgt tggaatggcc gtcataatca tgcgccgaat
    35101 ccgctcccca ttaaaatctg ggtctgtaaa gacaatgact ccatgacgtt ggtgtagacg
    35161 ctgaatccgc tctatgtcct ggtcattgat ggcagaacct cgagtctcat aggtctccac
    35221 atcgaaataa cgcttgagat tgaccgtatc atcacgacct tcaaccacga taacttggga
    35281 aattctctct ttcattactt gctgtccaat ccaaaaatgc gttctgcatt tgcagtcgtt
    35341 gctaccgcca gctcttctgt cgtcatacca cgcaagtcag cgataaagtc gaccacatag
    35401 cgagtatagg ctgttttatt ttcacgacca cgcttgggta caggtgctaa gtaaggcgca
    35461 tctgtttcta ccaacatctt gtccaaaggt aactctttag ctgcttcttg gaggtcagtt
    35521 gccttcttga aggtcaccac tcctgagaag gaaatggtca taccaagatc cacaaacttc
    35581 tctgcccact caagcgtccc tgaaaatgaa tgcatgatac caccacgagg accaacgccc
    35641 tcactcttga taatctcata ggtatcttcc agcgcatcac gggtatggac aacaaaaggc
    35701 aaatccaagt ccttagatag ctgaatctga cggcgaaaaa cctgctcctg cacctctttg
    35761 ggcgctgtca tccaatggta gtctaagcca atttcaccta aagccacaac cttggaatgt
    35821 tttaacttat ccaacaagta agcctcaact tcctctgtat aagtaccagc ttctgtagga
    35881 tgccaaccaa tagtcgcata gagctgctca tactcatcta ccaactccaa ggcatgctca
    35941 atcgtcggtt tatcaaaacc aacaatattc atctgtgtca cacccatctc agcagccaag
    36001 gcaatttctt ctgcctcacg acctgcaaat tcttctacat tcaagtgtgt atgtgtatca
    36061 aaaatcatct cttctaacct cgttttctat ccttctatta taccaaaaaa gaggaggggc
    36121 acctaatttt tcggtttccc ctcctctctt caatagagag ctattctgct atcttttcta
    36181 tccgatattg cccatctcct attccacagt tagagacaga agagattggc tacattgata
    36241 gaatattcct attcagctaa agtatgtgat tatcttgggt tctcactttt ctcacccatc
    36301 actaggattg atgacttctc aaaatccccc ttataatcta actttaagta gagaatagaa
    36361 ttttctagct gttctatttt ctacgtctag tctttcttcc tagcaaagca aaaccacttg
    36421 ttgccaccac tagaccaaga ctagcaagtg ctgcacttga tgctgaacct gtgttaggta
    36481 attgtttgcc agcaggtgac tggtaggtaa catcttgcat tgttcctgca gttgcttgcg
    36541 gtgcacttgt tgtcttagga gcagcattag gagccactgg tctcttagga actgtaacca
    36601 tggcaatggc ttgcactcca atcgtttctg cttccttcac atcttccata gttttcgcat
    36661 tttcaatctc tttgagagct gcttgttttt ctgcttccac tcttgctaaa agttctgctt
    36721 tttctttatc agaaagcggt gcgcctttga tttcatcctg cttgttcttg gctgcttttt
    36781 caatggcatt tttagctgat tctttttcag tagctaattg cttagaagct tgtgattgag
    36841 ctgagttttg tgaacctgtg tgagctgatc cacccgcctg gctaccaccg ccttcattcg
    36901 cattagcatc tgagcttcca gtattgcctc ccttatctcc acctggtgta ttttctagct
    36961 tatactctgg tgtagcttct tgagttacag attcaccttt cgcagattgt tgaactgtat
    37021 cttttcctag aatcgtcact actgaaccat ctgggaatgt gattgtcgct gtgccatctc
    37081 cagctacttc aactttatag taaaataaaa taataacagg acaaatcgat caggacagtc
    37141 aaatcgattt ctaacaatgt tttagaaaca gaggtgtact attctagttt caatctacta
    37201 taacatcgct tccgaatcca tttgcatcta ctgaactttt ttacaaaaat aaaaatcagt
    37261 agactcgagt tcctactgat ttttgataga attcgtttgg attatgcagc caaaactttg
    37321 cgtcctttac gacgacgagc tgccaatacg cgacgaccat ttttagttga catacggtta
    37381 cggaatccgt gtttgcgcgc acgacgaagt ttacttggtt gataagtacg tttcacgatg
    37441 aatacctcct catagatttt gtattcgttt agccggctat aaagtgatca gttactaaac
    37501 atactttact attctatctg attctttcac ttttgtcaat agccctaggc aaatgtttcc
    37561 agctttttcg aatgcaagcc ctatctacga tactgtttca agaaacgagt catcttttct
    37621 tggatttggg ctaactcatc aacacgagga aggtaaacga tacggaagtg gtctggttcc
    37681 tgccagttaa agcctcgacc atgaaccaag agaacctttt cctgcttcaa gaaatcaagg
    37741 acaaactgct catcatcatc gatacggtac atattgcggt cgatttttgg gaagatatag
    37801 agccccgcct tgggtttaac ggcagacaaa cctggaatat cttgaatggc attatagatg
    37861 aaatttcttt gctcgtagat tcgtccacca ggaagaagca attcatcgac tgattggtga
    37921 ccccccaagg aagtttgtac gacttgttgg gccaaaacgt tagagcaaag gcgcatattg
    37981 gacagcatat tgagcccttc gatatagccc ttaacatgag tcttagggcc agacaagacc
    38041 atccaaccca cacggaaacc tgctatgcgg tgggattttg acagaccatt catgctgaca
    38101 cagaagacat ctggtgccaa gctcgccaca ggcgtatgca catgtccgtc cattaccatg
    38161 cggtcataga tttcatccgc aaagataatc aaatcgtttt gacgggcaat ctcaataatc
    38221 tccagcaaga gttccttagg ataaagggct ccagttgggt tatttggatt gataaggacg
    38281 attgccttgg tattggaagt gatttttgac ttgatatcgt caatatctgg gtaccattcc
    38341 acagcttcat cacagatata gtgaacggca tttcctccag ctaggctgac agccgccgtc
    38401 catagaggat agtctggcat tggcactaag acctcatccc cattgtccaa aagcccctgc
    38461 atggacatga caatcaactc actgacacca tttccaaggt aaatatcatc aatatctacg
    38521 tttgggaatt tcttcagctg gcaatactgc atgatggcct tacgggctga gaaaattcct
    38581 ttggagtcag aatagccttc actatcacgc gcattcataa tcaagtcatg aatgacctcg
    38641 tctggcgctg taaagccaaa ttctgctgga tttcctgtat tcagacgtaa aatcttttct
    38701 ccattcgctc gcatccgcat ggcttcttcc aatacaggac cacggatatc ataagcaaca
    38761 tgctctaact tactagactt gttatattct ttcatcttgt ttcctcaatt catcaggata
    38821 gtaacattat accactagaa agagaatgcg acaagtttac tgaaatgtgt tactagtttc
    38881 gtatagaaga aaaaccttgc taaaggcgag gttaagaacg tagagatagc aggtattttg
    38941 caaagacaca tcacatacca aaagataaac ctagaaaaag caagcctaga aaggagtcat
    39001 aaagaagatt aaacatgagg aaaagccccc atatcatcaa gtaatcccaa cgcccacttc
    39061 gttttgcaac tagaaataag attagatggt agagtacatg aaaaactaaa aatccaataa
    39121 aacttggata aagaagcggt accagactct ctaattgaca gatcatgatc acttctacca
    39181 agactgaaac taggataatt ccataataaa ggaaatttga tttttgaaaa gagtttttca
    39241 tcattctccc ttccttcact tctttcctgc tatcctttct tttattttat catatattta
    39301 tatagaattt aagccctaat attcttttga aaaatacttt ttttgcttta cttctatagt
    39361 gaaaaggttt acaattatag taagaaaatt tcaggaggtt tcattatggc acaacgttac
    39421 caaaatatca tggtcgcaat cgatggttct aaggaagcgg acttggcttt tgtcaaggga
    39481 gttcattctg ctctacgaaa cgacgctaaa ctcaccatcg cacatgtcat tgacacacgc
    39541 gctctccaaa gcgtatccac ctttgatgct gaagtttacg aagaactcca agtcgacgct
    39601 gaaagtctga tgaaagagta cgaaaaacgt gctaaagatg ctggggtagc agatgttcat
    39661 atcgtcattg aaatgggaaa tccaaagacc ctgctagcac gtactattcc agatgccgag
    39721 gaagtggacc tcatcctcgt tggcgcaact ggtctcaacg cctttgaacg cctcttggtc
    39781 ggctcttcat ctgaatacat actccgccat gctaaggtcg atttgctggt tgtgagagaa
    39841 caagaaaaaa ccttataatc acaaagaaaa ggagccccta gctccttttt gtttacgatt
    39901 tatttctctc tttatggcgt tcgtaagcct tgagctggcg ctgcagttcc tttttaatag
    39961 caggttctgg agcatatttt tcttcccaat tatctggttt taagatttta tgggtcactg
    40021 gatcaaaatg agccttgcca tctggaaaaa ttttccccat attggcctga tggacaatat
    40081 caaaaatacg ttctgggtcc acccccatca agacaaaact gccgtaggtg aagtaaagcg
    40141 tgtcaatcaa ggcatccact tgccctatca aatcttgctg agcaggtgtc ttcttggcta
    40201 ctttatctgc tgccttatca agggcctgat gaagttgcga tacagcttga ccaaaatctt
    40261 cttcagaagg actggctgct cgaacaaact ccaccaattc ttctatttta aaaccagccc
    40321 tatgggttgc accctctaaa tcccaagctc gaggttcttc ttgggttcgt tcatccatca
    40381 tgtggtggaa ggtcttgacc ttattgaaat gatagtcacg gctgacaaag actttttctg
    40441 aagacaaaac accaaaatgt tccaaggcct tgtggatccc atcttgttga ttgctagtgg
    40501 taatgtgctt ggcagcttcc ttgacactgc tactaccatt tcccatagcg accgacatac
    40561 cgacgccagc cagcatttca aggtcattat ctgagtcacc aaaagccatg acttgattga
    40621 ggtcaaagcc atattctttc ccaactcggc gaatgccttc taatttagaa tttccctgat
    40681 tgatgacatc cgatgcaaaa ggattgctac gtgtcaattt caagtcttca aaatcagctg
    40741 ccgccttctc agattcttct ggtgtcatca gcatcaaaac ttggtagata ggctgattca
    40801 tcaggtgaag caggtcctct tccttttggg gaacaacctt gctgaccatg cgattaaaag
    40861 actgactcac cgtccgagtt aaaacagagg gaacgaagcg actaattcgt tgggaaaaag
    40921 aacccagacc aaaggacatg attttagaac ccaacatggc atccttggtc cctagggcaa
    40981 tctccgtgcc ctctttttta gcatagctaa ttagatggcg caaatgtaac ttggaaatag
    41041 ggctcgtgaa caagactctg tctttactaa agatatactg gccattatag gttaccgcaa
    41101 aatccagatc caaatcgtcc atcaattcct taacaaaaaa aggtcctcgc cctgtcgcta
    41161 cgccaactag taccccttgt tctttgacaa tcttaatcgc atccttagtg gatttcaaaa
    41221 cactcttgcg attgttgacc aaggttccat cgatatcaaa aaaaacagct ttgacttcca
    41281 tcctatccca atctcccctt ttgtgataca atgattatac cacatttcag aaagagttag
    41341 taaatcatgc ctaagaaaat ccttgtttta catacgggtg gaactatttc catgcaggcc
    41401 gatgcttctg gcgctgttgt gacgagttca gataatccca tgaaccatgt gtccaaccca
    41461 cttgaaggaa tccaagtcca cgccttggac ttttttaacc ttccaagtcc ccatatcaaa
    41521 cccaaacata tgctggtcct ctaccagaaa attaaagagg aagcagataa ctacgatgga
    41581 gtggtgatca cacacggaac cgatacttta gaggaaacag cctatttcct tgataccatg
    41641 gaagttcccc acatgcctat cgttctaaca ggagccatgc gtagctccaa tgagctcggt
    41701 agtgatggtg tttataatta cctaagtgct ttacgagtgg ccagtgatga cagggctgct
    41761 gacaaaggag ttttggtcgt tatgaacgat gaaatccacg ctgccaagta tgtcaccaaa
    41821 acacatacga ctaatgtcaa caccttccag actccaacac atggccccct cggtctcatc
    41881 atgaaacagg aaatcctcta cttcaaaaca gctgaacctc gtgttcgctt tgaccttgat
    41941 cacatacaag gtttagtccc tatcatctcg gcttatgctg gtatgacaga tgagctgatt
    42001 gatatgctgg atttagaaca cttggacggt ttgattatcc aagccttcgg agctggtaat
    42061 attcccaaag aaacggctca aaaattagaa agccttctgc aaaaaggaat cccagtcgct
    42121 ctggtatcac gatgctttaa cggtattgcc gagcctgttt atgcctacca gggtgggggc
    42181 gtacagttgc aaaaagcagg cgttttcttt gttaaagaac tcaacgccca aaaagcccgc
    42241 ttgaaactcc tcatcgccct caatgccgga ctaacaggac aggctttgaa agactatatg
    42301 gaaggctaat actcttcgaa aatctctgca aaccacgtca cgtcgcctta ccgtatgtat
    42361 ggttactgac ttcgtcagtt ctatctacaa cctcaaaaac atgttttgag ctgacttcgt
    42421 cagttctatc tgcaacctca aagcagtgct ttgagctgac ttcgtcagtt ctatctacaa
    42481 cctcaaaaca gtgtgttgcg cagcctttaa ccagccgcct agtccgctct atggtattca
    42541 ttaagtcaac atctcttgtt taagagcaca aaatcaggaa atcttctcga ttccctgatt
    42601 ttttctattt acgttttcgt gttgagctac gttctgtcaa accatgaggt aagagaactt
    42661 cacgttcttc caactcttcc ttatgcataa tcttggtcaa catacgcata ctaatggcac
    42721 caaggtcata aagaggttgg gcaatcgttg tcaagtttgg acgggtaaag cgtgagattt
    42781 gtgaatcatc actagtaata atttcaaaat cttctggcac agaaacaccc ttatcagcca
    42841 aaccgttcaa gactcctgct gccaactcat cacctgtcac aactgctgca gttgcatttg
    42901 atgaaatcaa acgctctgct aaggcgtaac catcatcata gctatattta gattcaaata
    42961 ccaaaccctc actataagcg attcctgctt ttttcaaggt ttccttgtag ccaactaaac
    43021 gaaccttacc attgatgtca tccactaacg gaccgctaac gaaagcaata cgctcatttt
    43081 ctttagcaag gtaactcact gcatcaattg ttgcttgctt atagtcaata ttgacacttg
    43141 gcaactggtg ctcaacatcg acagttcctg cgagaacaat cggagtacgt gaacgcgaaa
    43201 attctgagcg aattttatct gtcaagtgat aacccatata gataatgcca tctacctgct
    43261 ttgaaaagag ggtattgaca acagaaactt ctttctcgtt atcttcatcg ctattagcta
    43321 ggacaatatt gtacttgtac atttctgcaa tatcatcaat ccccttagcc aaactcgaaa
    43381 aataaccatt ggtaatattt ggaatcacga caccgacagt ggttgtcttt ttacttgcaa
    43441 gaccacgcgc aactgcattt ggacgataat ccaagcgatc aattacctct agcacttttt
    43501 tacgggtatt ctcttttaca tttttattgc cattgaccac acggctgacc gtcgccatgg
    43561 aaacacctgc ttcacgagcg acatcataaa tggttactgt atcatctgca ttcattcctt
    43621 ttcctgtcct ttctatctca cacattcttt tacaagtaga ggtactgatt gaagctctat
    43681 atctacttac aaaagtgaag atgtgaaaat ttcgttttca tatttctact tattccattc
    43741 tatcactaat tgtaaacact ttcaagtgtt ttttgaagat tgattgaaaa aatttcatag
    43801 aaaacctagg tttagctcct tgctaccacc ttagactaaa caaaacggag gaaactaagc
    43861 cctcctaaag ttatagtaaa atgaaataag aacaggataa atcgatcagg acagtcaaat
    43921 cgatttctaa caatgtttta gaagtagagg tgtactattc tagtttcaat ctactatagg
    43981 tattgttcca ttcactaccg tcaattttag cacatagtct tcatgaaaat attatatcat
    44041 cataaccaac cagattcttt tgcgatatta gctgcctctg ttcgattacc agcatccaat
    44101 tttgaaagaa tattggtgac atagtttcga atagttccgt ttgagaggta aagctgatct
    44161 gcgatttctt ggttggataa gccccgggcg atccccttta agacagcaat ctcttgttct
    44221 gtcaacggat tgggacgggt catcaccact tccatcaatt caggcgaata ctccttgcgt
    44281 ccttcgagga cggtgtgcaa ggtttgcatg aggtctgcaa cgcttcgttc ctttaataca
    44341 taagcatcca ctccagcctt gaccgcacgt tcaaaatacc cagcacgctt gaaggtcgtc
    44401 accacaacca cctttgtttc aagcctttct gatcgtatcc actccaagac ttcaagacct
    44461 gtcttaacag gcatttctac gtcaaggatg gcgatatcta cagactcctt ttctaatagt
    44521 tggattgctt cttgcccatt cttggcttga aggacagact ccacatctgg ttgaagcgtg
    44581 agcaattgac acatggcatc tcgcaacata ctttgatctt ctgcgactaa tactttcatc
    44641 ttagtttctc tccttataag gtagtcgaac ttgcacttcc gttggatgtt tctgactgat
    44701 tacacttact tctcctgaaa atggaaaaac acgatctcgg attgtatgga gctcatctcc
    44761 ttttagaaaa gcaaagccgc agccatcatc acttactgtt aaaatgagtt ccttctctgt
    44821 ccgttctaat tttaagtaag ctttagacgc tttggcatgt ttgatgatat tggtcaccaa
    44881 ctcaagcaaa atcatagaag ccattgactc caattcttga gtaaggctag cagtatctag
    44941 ttggttatct gtttccacct caattccagc aatttctaac atctttttca cagtctctag
    45001 ttcggatgtc aaagttctag acttaagatt ttccacaatg gttcgaactt cacgcattga
    45061 ttctttgcta atttgctgta tttctcttaa ttccttttcc acttgtggat aagcctgcat
    45121 ttgaaatagc tgcaaggcta gatctatctt gacactaagc atagcaaagg tatgtcccag
    45181 actatcatgc aaatcctgac cgatacgatt gcgctcattt tctgctagca atagatttat
    45241 ctgggtattt tgcttgacct gagcttcttt caaatcctcg acaaggcgaa ttcgaaccga
    45301 gcccaaagtc atcaaatcga caaaagtaag aattacaagc atatagacta gaaactcaac
    45361 ttcgattctc tgaaaaatca aaagtcgccc cacaacaagg acttgagcaa ggagaaatgt
    45421 ccatacatgt aaagacttta aactacgtac gccaaaatga taaattaaga gattggaaag
    45481 gaaaaagaaa aaccagatat aattcccagc aacaaaggcg gtattcccag ctacatagac
    45541 cagcatgagc ccccagaaaa gccaagatag gcgctggttc ttagttatta aaacacccaa
    45601 atacgtcact acaaatagaa tatcaaccaa taaatgccaa gcaggaagct ccccaatcac
    45661 tacaggtagg atgggaaaaa gcataaaaat caaactggcc caaaacatat agtgtatgct
    45721 tttcagtctt tcaagcatta agcattctcc ttatgacctt gaaggtaaat agtcaaacca
    45781 aataaaactg ctgaaaaaac aagtaaataa actgtggctg atagattgat gccaccctcg
    45841 tttaagaagg tcttgagcaa ctccatcaat tgataggtcg ggagacactt accaactgct
    45901 tgcatccagt ctggaaataa agaaatgggc atccagagtc cgcctaaaac agccaatcct
    45961 agataaagaa gattgcccac aacagacatc agctgactag ttggtaagag agtcaaggtc
    46021 aagccaagtg ctacaaaagc aatactcccc acaatcagca aaatcgcagc tccaacccat
    46081 tttactagag gcatgtctac acctcttaca aaatgtccaa ctgtaaaaac aaccagaatt
    46141 gagaccaaat aatcaaccag catacttgtt atctttgata gataatattc taccatattt
    46201 acagggctat gacgcaatgt tttctgccag ttgttcatct tatcggtatg taaaacagct
    46261 ggaaatgaga ggatagttgt cgacatcatg gaaaaggcag tcatggaaat gaggtaatca
    46321 cgcataaaat tcgcgagttc acctggtgtg tcctgataga taccagaaaa aaataaatag
    46381 aaagccgtcg gcatccctac cgacaataga taatagatta attgtcgttt ggtcaataaa
    46441 aattctatct tactaagtgc tatccatcgt ttcatcttag ttatctccct tctgcgtctc
    46501 ttcaaagatt gtattcaaca aactacgatt attaacttcg atttcttgca tcctacatcc
    46561 tgcttgggct aatagttccc agaaagcatc tgcttctcga gtgactactt gtaaagaatc
    46621 ttgctttagg gtccagtttt caaccaaatt tgactgctct acgacttcct tgtaagctat
    46681 aggaaggata aagtgctttt ctatttcctc gctacgcata gctagaggcg tcgtatctcg
    46741 aatcaactct cccttattta agagcaaaat gcggtctgca gtgtgttcca cttcctcaat
    46801 ataatgggac gaatagagaa tggtgactcc ttgcgctttt aactcctgga caatttccca
    46861 aaaacgttga cgagttgagg tatccatgga cgcagtaggc tcatctaaaa aaacaatctt
    46921 tggtcgccca attaaggtca agacgaaaga aaagagacgc ttttgaccgc ctgataattt
    46981 ttctgccaac tgttcttttt gttgcttgac aaactgcaat agttgatcaa tttcctgatt
    47041 gctcaaagga tttggataga tacgttgaaa gaaagcaatc aactctttaa cttttaattt
    47101 ctgaacgatt acattttctt gagggaggta agccctagta tagtctaact tagaactcgt
    47161 cactggtaaa ccttgaatag atacttgacc gcttgtgacc agtttatctc caagcaaaca
    47221 atctaagagt gttgtctttc cggcaccatt tggaccaatt aaggcaacac attcaccatc
    47281 gttgatttca aaggagatat tccgcaaaat ttccttgtct tttattttct tactcaattt
    47341 ctcaacttta accaccgtca tgagactctc ctttcaacca ctccattccc ataaggaaaa
    47401 cgacgaaaat cataaatcca aaccccaaag caccacgaat gaattggcga agcaaggctt
    47461 gatcaaacca atcagtaaac atttccacta accataccaa gagtgacagg ccgataaaga
    47521 aatagatgat ccctctcttc attcctcaaa ctcctttttc acatctctga ctaatttcaa
    47581 accttctcta acaagccaag acatgattcc aaagccagca aagaccttcc aaggaaaatg
    47641 gtagaaatcc tcatccaatc ccgaaaacat gagataggtc ataactcctg ctactactaa
    47701 acttactgcg ataatcattt tatttctcat ctcttcttcc cccatttcat actacaatta
    47761 tagtctttta aaatcagagg agacagaagc ttctgtcact agaaaatatg acaaatgtca
    47821 taaaaaattc tgttcaaaac aagcaagata cactatacaa taaaacacaa tcagaaaaat
    47881 ctaaggcaac ttcctcaaaa gagatatcaa acccaattca caccataatg taaactaata
    47941 cttatttaaa atcaaaaaga gtaaaaattt ttatcag
//
LOCUS       DAVKQL010000007        47949 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVKQL010000007 DAVKQL010000000
VERSION     DAVKQL010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 47949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47949
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.007"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(377..2104)
                     /gene="ezrA"
                     /locus_tag="VW561_000323"
     CDS             complement(377..2104)
                     /gene="ezrA"
                     /locus_tag="VW561_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEU9942658.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2186..4132)
                     /gene="gyrB"
                     /locus_tag="VW561_000324"
     CDS             complement(2186..4132)
                     /gene="gyrB"
                     /locus_tag="VW561_000324"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEU9942659.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEIVGDTDKTGTTVHFTPDPKIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKREG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4147..4710)
                     /locus_tag="VW561_000325"
     CDS             complement(4147..4710)
                     /locus_tag="VW561_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9942660.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEVISNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4795..5349)
                     /locus_tag="VW561_000326"
     CDS             complement(4795..5349)
                     /locus_tag="VW561_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEU9942661.1"
                     /translation="MVKVAVILAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYQDVFGKNQ"
     gene            complement(5360..6583)
                     /locus_tag="VW561_000327"
     CDS             complement(5360..6583)
                     /locus_tag="VW561_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU9942662.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6620..9070)
                     /locus_tag="VW561_000328"
     CDS             complement(6620..9070)
                     /locus_tag="VW561_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEU9942663.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLELRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDDPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SHSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9270..10199)
                     /locus_tag="VW561_000329"
     CDS             complement(9270..10199)
                     /locus_tag="VW561_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU9942664.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELLVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVK"
     gene            10339..10626
                     /locus_tag="VW561_000330"
     CDS             10339..10626
                     /locus_tag="VW561_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEU9942665.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10660..11994)
                     /gene="ciaH"
                     /locus_tag="VW561_000331"
     CDS             complement(10660..11994)
                     /gene="ciaH"
                     /locus_tag="VW561_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEU9942666.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(11984..12658)
                     /gene="ciaR"
                     /locus_tag="VW561_000332"
     CDS             complement(11984..12658)
                     /gene="ciaR"
                     /locus_tag="VW561_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEU9942667.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12767..15313)
                     /locus_tag="VW561_000333"
     CDS             complement(12767..15313)
                     /locus_tag="VW561_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU9942668.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEARQVASQIFATHKENLAELPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLTAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15436..15876)
                     /locus_tag="VW561_000334"
     CDS             complement(15436..15876)
                     /locus_tag="VW561_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEU9942669.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDIILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTSESKVVLEEFRKVYPL"
     gene            complement(15851..16720)
                     /locus_tag="VW561_000335"
     CDS             complement(15851..16720)
                     /locus_tag="VW561_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEU9942670.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFSELTNTEANCLVGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSVIFPFVNDDDQLKQAITVLRQQGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKIDFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(16698..17198)
                     /locus_tag="VW561_000336"
     CDS             complement(16698..17198)
                     /locus_tag="VW561_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9942671.1"
                     /translation="MEIKKHFGVYCVCFENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETRFTVRSYSNPRIYDVFVREELKNFMVHHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVND
                     EKSTCP"
     gene            complement(17209..17907)
                     /locus_tag="VW561_000337"
     CDS             complement(17209..17907)
                     /locus_tag="VW561_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEU9942672.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(17900..18136)
                     /locus_tag="VW561_000338"
     CDS             complement(17900..18136)
                     /locus_tag="VW561_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEU9942673.1"
                     /translation="MTFEEILPGLKTKKKYVRTSWGGAENYVQLFDTIEQNGLALEVT
                     PYFLINVSGEGEGFSMWSPTACDVLATDWVEVHD"
     gene            complement(18236..19162)
                     /locus_tag="VW561_000339"
     CDS             complement(18236..19162)
                     /locus_tag="VW561_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEU9942674.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFDLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCKLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(19237..20052)
                     /locus_tag="VW561_000340"
     CDS             complement(19237..20052)
                     /locus_tag="VW561_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942675.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(20056..20376)
                     /locus_tag="VW561_000341"
     CDS             complement(20056..20376)
                     /locus_tag="VW561_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU9942676.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            complement(20583..20663)
                     /locus_tag="VW561_000342"
                     /pseudo
     CDS             complement(20583..20663)
                     /locus_tag="VW561_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(20707..22704)
                     /gene="metG"
                     /locus_tag="VW561_000343"
     CDS             complement(20707..22704)
                     /gene="metG"
                     /locus_tag="VW561_000343"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU9942677.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFCLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(22828..24087)
                     /locus_tag="VW561_000344"
     CDS             complement(22828..24087)
                     /locus_tag="VW561_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9942678.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNVDEIANIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(24089..24790)
                     /locus_tag="VW561_000345"
     CDS             complement(24089..24790)
                     /locus_tag="VW561_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9942679.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(24774..25973)
                     /locus_tag="VW561_000346"
     CDS             complement(24774..25973)
                     /locus_tag="VW561_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU9942680.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PTHLVVANEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAQAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            26259..27605
                     /gene="gor"
                     /locus_tag="VW561_000347"
     CDS             26259..27605
                     /gene="gor"
                     /locus_tag="VW561_000347"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEU9942681.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFNFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESVAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTSSEEFVTMR"
     gene            27801..28337
                     /locus_tag="VW561_000348"
     CDS             27801..28337
                     /locus_tag="VW561_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEU9942682.1"
                     /translation="MKKAHVYAIPAIGAALIAVLAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLYFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQTYFTN"
     gene            28393..28473
                     /locus_tag="VW561_000349"
                     /pseudo
     CDS             28393..28473
                     /locus_tag="VW561_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(28542..28757)
                     /locus_tag="VW561_000350"
     CDS             complement(28542..28757)
                     /locus_tag="VW561_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEU9942683.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            28855..29175
                     /locus_tag="VW561_000351"
     CDS             28855..29175
                     /locus_tag="VW561_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942684.1"
                     /translation="MRHKFQQVLDKIHDFLNGHDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFSKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(29205..29543)
                     /locus_tag="VW561_000352"
     CDS             complement(29205..29543)
                     /locus_tag="VW561_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942685.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(29555..30274)
                     /gene="trmD"
                     /locus_tag="VW561_000353"
     CDS             complement(29555..30274)
                     /gene="trmD"
                     /locus_tag="VW561_000353"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEU9942686.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(30264..30782)
                     /gene="rimM"
                     /locus_tag="VW561_000354"
     CDS             complement(30264..30782)
                     /gene="rimM"
                     /locus_tag="VW561_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEU9942687.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(30813..31601)
                     /locus_tag="VW561_000355"
     CDS             complement(30813..31601)
                     /locus_tag="VW561_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9942688.1"
                     /translation="MKRFEVSTEIGSLSVAYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIYDQAGKNLDNLADVVYEALEELGISEYILCVHSWSGI
                     LACKLLEKPIKCQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFENLSIESHVPVFIFCQT
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
     gene            complement(32178..32417)
                     /locus_tag="VW561_000356"
     CDS             complement(32178..32417)
                     /locus_tag="VW561_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEU9942689.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(32437..32709)
                     /gene="rpsP"
                     /locus_tag="VW561_000357"
     CDS             complement(32437..32709)
                     /gene="rpsP"
                     /locus_tag="VW561_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU9942690.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            32879..33088
                     /locus_tag="VW561_000358"
     CDS             32879..33088
                     /locus_tag="VW561_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942691.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
     gene            33567..33779
                     /locus_tag="VW561_000359"
     CDS             33567..33779
                     /locus_tag="VW561_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942692.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEDFQDDDEIPTII"
     gene            33863..34666
                     /locus_tag="VW561_000360"
     CDS             33863..34666
                     /locus_tag="VW561_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU9942693.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIGGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(34723..36264)
                     /locus_tag="VW561_000361"
     CDS             complement(34723..36264)
                     /locus_tag="VW561_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9942694.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMTEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGIMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(36266..36739)
                     /locus_tag="VW561_000362"
     CDS             complement(36266..36739)
                     /locus_tag="VW561_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU9942695.1"
                     /translation="MTKKRELILRLGVAVYSLCIVYFCFTPQATVEVGTPGIQHLGRL
                     VFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKKVLL
                     FSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN"
     gene            complement(36741..37826)
                     /gene="rlmN"
                     /locus_tag="VW561_000363"
     CDS             complement(36741..37826)
                     /gene="rlmN"
                     /locus_tag="VW561_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEU9942696.1"
                     /translation="MKPSIHSLTHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQGIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFAERGQDERVNHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMAIGARHITVSTSGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALE
                     LAELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGGNCVVRQEHGT
                     DIDAACGQLRFNTMKRDRQKAVAAVNT"
     gene            complement(37847..38377)
                     /locus_tag="VW561_000364"
     CDS             complement(37847..38377)
                     /locus_tag="VW561_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEU9942697.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKGQRHFVIRQK"
     gene            complement(38534..39139)
                     /locus_tag="VW561_000365"
     CDS             complement(38534..39139)
                     /locus_tag="VW561_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEU9942698.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            complement(39312..40349)
                     /locus_tag="VW561_000366"
     CDS             complement(39312..40349)
                     /locus_tag="VW561_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU9942699.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQMLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKADSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            complement(40383..41318)
                     /locus_tag="VW561_000367"
     CDS             complement(40383..41318)
                     /locus_tag="VW561_000367"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEU9942700.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            complement(41880..43070)
                     /locus_tag="VW561_000368"
     CDS             complement(41880..43070)
                     /locus_tag="VW561_000368"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEU9942701.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            complement(43285..44628)
                     /locus_tag="VW561_000369"
     CDS             complement(43285..44628)
                     /locus_tag="VW561_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9942702.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLESNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            complement(44768..45583)
                     /locus_tag="VW561_000370"
     CDS             complement(44768..45583)
                     /locus_tag="VW561_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU9942703.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASLLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAILNWK"
     gene            complement(45635..47815)
                     /locus_tag="VW561_000371"
     CDS             complement(45635..47815)
                     /locus_tag="VW561_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9942704.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFTPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGTAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVGLEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
BASE COUNT        14211 a        10485 c         8658 g        14595 t
ORIGIN      
        1 tgcaaataag aaatctccag attaggaact atccgtgagt tctctagtct ggagattttt
       61 caatagactt cgttattgga cggttaccaa gttactcaaa gtacagattt gaagctgcag
      121 ataagactga cgaaatcact ttaatactaa atgaaaatca aaaagcaaac taagagcctc
      181 aggctgtact tgagtacggt aaggcgacgc tgacatgatt tgaagagatt ttcgaagagt
      241 ataaaacact gttttgaggt ggtggataaa tcggctgggc aaaaactcaa tctcaggaga
      301 aaatgaagta aatctcccca caactataga aaaagatttg attctgctcc tcacacaata
      361 aaatcttttt cttttattaa aaacgaatcg tttcacgtgt tttctcatat gaggtaacaa
      421 agcgattggt tacaccaggc tctgccactt ccaatgcttg agaaatcttg tcaaatgaag
      481 cgtgataatc aaattctttt tcaaaaatat ctaaagcttc gttaaatgct tcttgaatgc
      541 gttcatcaaa tgagcgatag cggttagaat attgcaagag ttgctctgtc aaagttgcat
      601 attgtacaat attataagtt tccgtttcta aagcttccat atcattcgtt gcaatttcaa
      661 gaactcgggt aacagattca atgttaatca ttttttgttc taactcaacc attaaatcct
      721 cggtattatt gcttgccgta aagaataact tcaagaaagt ttgtggaata cctggcagat
      781 tgcgtttttc catgtatcgc ttgatagtat ggagacgatt gacataaaca ttggcctttt
      841 gacgtgcatt aatatcatct ttctcaattt gtgtcaggcg ctcactaact gaaatttgct
      901 catcttcaat atcttttagt tgagtttgta aatcctcaag attttcttca agaactgaat
      961 aagcttgggt tggttcttct tgatttgaag ttacctcaac aatagctgcc tcaaaactct
     1021 ctaattctgt ctgaatacga cgaacatggc ttgcagctgt ctcaggaagt aaataggtct
     1081 tgttcaaacg tgcaatatct tctcccaata aagtattatt ctctttcata tgttgaaggt
     1141 aagttggaag agttgcaagt agattttcca ctactttctg agcagcaatt tctcgagtaa
     1201 aaatatcata caaggcattg atttcctctt gtgcctgtcc attctcatat tcggcattat
     1261 ccaattccaa ctgacgaata ttctcttggt ttttcttgaa tgcttcataa agcaagtgga
     1321 aacgcgcttc aatatccgtt tcaacaaaat gataattagc atcaattagt ttacgataac
     1381 cggcttccaa atcctgtaat tgatctggca attctgtaga aagcgtcgta accaaggctg
     1441 gaacacgatc cacaatatga cttaaggcca aaatgtgatt ttctgtatta tccaaaatca
     1501 ctgcggcttc cacagggtca cccgatgaat tcaaggttac aaattgtgaa aattcagatt
     1561 ggatattttc taattgtttt tcaatttcat ccaaggcttg accatactgt tctgaatttt
     1621 cagcaactct atgctgaagt tcctcaaata aatccaaagc atgaagaaca cgaccactat
     1681 ttttagattc ttgcttctct aagtctgcca aagcattgcg aattgccgca atatcttctt
     1741 caatcaaagt aatttgactc tcaatttggt caatttgatg actggccttg agaaaacgaa
     1801 atgaatggtt ataaccttct gcttcaaaga gattattttc aatatcggca aaagagttga
     1861 gagataaatc gacccatttt tgattccatt cacggaaagc cacttgactt tgtccaatca
     1921 agtgcatatt ttttacagct tctacttcat catttactgg aagattgtat agttcttctt
     1981 ttctttcttc tagcgcctct aatctcccct cgtttcgctt acgtagaaag attgccacta
     2041 cataagccag aactaaaatg actgcaattg caaccattaa ataaattagt tgtccattag
     2101 acatatcaaa ctcctttttt acttgaaaca atcgtaacga ttatatcata ttttttagac
     2161 caagggaact acttctccct attttttaga catcaagtgt actatagaca gcattttctt
     2221 cgataaactc acgacgaggc tctactcgat cccccatcaa catatcaaag attttatctg
     2281 cttctgcagc atcatctaca gaaactctag ccatcaagcg atgttcggga tccatggttg
     2341 tttcccacag ctgatgatcg tccatttcac ctagcccctt ataacgctga atagtcggtt
     2401 tggtacgacc ttcactataa cgggctaaag cttcttggag tttgatttct tgatctgcac
     2461 ccggctggat atattcttta atctcgcttc caaccttgac accatagatt ggtggttggg
     2521 caatataaac ataaccagct tctaggattg gtttcatata acgataaatc aaggttaaaa
     2581 gaagggtacg aatgtgggct ccatcgacat cggcatcggt catcaaaacg agtttttggt
     2641 aacgggcttt cgaaacatca aattctgcgc caaatcctgt tcccatggct gtgaaaagac
     2701 tacgaatttc ttcgttagct agaatcttat ccatacttgc tttttcaacg ttcaaaatct
     2761 taccgcgaat tggaaggata gcctgaaact cacggttacg accagatttg gctgatccac
     2821 cagctgagtc tccttcgacg atgaagagtt ctgtttcagc agggttatta gaagaacagt
     2881 ctgctagttt ccctggaagg ttggaaattt ccaaaccaga ttttttacgt gtgacttcac
     2941 gcgcacgctt ggcagccaca cgagccttgg cagctaaaat ccctttttcc acgatacgtt
     3001 tggcaatctg tggattttcc atgaggaaat cggagaaggc ttcactgaag aggcgattgg
     3061 taatcttgac cacttcgcta tttcccaatt tggtcttggt ttgtccttca aactgtggat
     3121 ttgggtgttt aactgagata actgcagtta agccttcgcg aacatcttcc cctgttaggt
     3181 tgtcttcatt gtctttcagt aacttatttt tacgagcata atcattgata acacgtgtca
     3241 aggctgtacg gaaaccttgt tcatgcgttc caccttcatg tgtatgaata ttattggcga
     3301 aactcatgac attttcatgg taaccagttg tgtactgcat ggctacctca actgtgatat
     3361 catccatctc accgtctgta tagattggtg tatcaaagat tacatccttg ttctcgttga
     3421 tatattcaac gtaactagca atcccacctt cataatgata atgcttggtt tgttccaaac
     3481 cttcacgctt atctgtaatt gaaatttgaa gaccgcgatt tagaaaggcc aactcttgaa
     3541 tccgtttatt taatttatca aaatcaaaga ttgttgtttc agtgaagatt tttgggtccg
     3601 gtgtgaagtg aacagttgtt cctgttttat ccgtatctcc aactatttca agatctgcga
     3661 caacatgacc acgacggtat tcttggtaat gaatcttacc atttttgtga acatgaacgt
     3721 ctaattgagt ggaaagggca ttaactactg acgaccccac cccgtgaaga ccacctgaaa
     3781 ccttgtatcc accaccgccg aactttcctc cagcgtgaag gactgtaaag acggtctcaa
     3841 cagcaggacg gcctgttttt tcctgaatat cgactgggat accacgccca tcatccacaa
     3901 cagtaatcga atcatctggc tcaataaaaa cttgaatatg gctggcaaat cctgccaagg
     3961 cctcgtcaat tgagttatca acaatttccc agactagatg gtgaagacct tcttttgagg
     4021 ttgatccaat gtacatccct ggacgcatac gaacagcctc taagccctct aaaacttgaa
     4081 tttgactggc atcataatcc tgtgcctgca gatttttgat ttcttctgtc atcttattcc
     4141 tttttcttat atgtctaata cttgtcttaa attcacgata ctggtaaaca agtgggtatc
     4201 aagtcctgca gcttgacctg cttcgatatc aatcggccga tcaccaatga caagaccaga
     4261 gctaatctga tacttttctc ttaaataaag catggattcg ggatttggct ttctcttaaa
     4321 gcctgagcta gaagtcacca cttctgtaaa ataagctgct atagaggttt tttctaaaat
     4381 ttccaaaacc tgatcatttc gatgagagac caaaaaatga cggccacctt gatttgaaat
     4441 gacttccaat aggtcagaaa ctccttcaaa taaaatcggg tgttcaagct ctctggcttc
     4501 attttccttg tacttttcta aaaaattctc taaattggga gcgaatgtct caatcgcaaa
     4561 aggagtagaa acctttaaag cttgatagac actgtcatgg tcttgtgtga taccatacag
     4621 tgccaatgtt tcaacaaatg cagctgttga agtttcataa ttatccagta aagttccacc
     4681 taaatcccag atgtaatcgt gatatttcat acccttcatt atagcatttt tttatgaaaa
     4741 aagcgatgat ttccctgagt tttccacata aaaaacgaga gaataactcc cgttttactg
     4801 atttttacca aagacatctt gatagagcat tcctgttcgt aaactctctg cgtcccctcc
     4861 tagttgctcc accaactcgt aggcaaaggc aagggctgtt gaaggacccc gactggttgt
     4921 caactgacca tctaccacta ctgtttcctt gacatagtga ccatcaagga tttgctcttg
     4981 aacgccgtca taacaagtgt atcgcttatt tttcaatatc tctgcttgat tgagggcaat
     5041 tggtgccgca caaatggctg ctagtttctt cccttcttgc tcgaagcttt gcaattcttg
     5101 aatcaaggtc tgattatcac gtaaatgtgc agaaccaggc atacctccag gaagaacaat
     5161 catatcatag tctgataaat ctccatcaaa gacatgatct gctcttactt ggattgcatg
     5221 cgaacccgtt acttgctctt caaaaccaac catatcacat gtgatattgg ctcgacgcaa
     5281 gacatctaca actgtcaagg cttcaatttc ttcaaagccc tgagctaata taactgctac
     5341 ttttaccatg attttctcct tatttaattt gttttaatac aacctttttc cgtttgaatg
     5401 gaacttttcc gctcttttct tcagctagat tagtctggta actcatcgat aaaagcaaac
     5461 caacaccaat cagattactg ataatagctg atcccccttg cgaaatgaaa ggcaagggaa
     5521 tccccgtcaa aggaagtagt ccagtcacag caccgatatt ctcaaagatg tggaagagca
     5581 acatcataat caaacctgtg gaaatataag tgtagaactg gttatttgat ttaagagtaa
     5641 tcttcaacat acggtaaatc aacatgagat agagggcaat aaccaggaca gagccaataa
     5701 agccaaaatc ttctgcaata accgtaaaaa tcatatctga ctctcgaact gggataagca
     5761 gattcgaagc attaaatccc tgaccaaata agccaccact cccaatggca atctgccctt
     5821 gagcctgctg gtaagtcgtt gtttgggcaa actcaaaggg attgagccaa gccaaaatcc
     5881 gattgatttg gtaggtcggc attccaatct ggtgaagaaa agctcgtccg tccttgctaa
     5941 taaagatagc taagaaacca gcaactcctg ttacagcagt cacaaatact gggataataa
     6001 ttttccaaga aacccctgat aataaaacga ttcctgagaa aatggctaca aaaaccaaag
     6061 ccgtccccaa gtcactttga agtgctaaaa gaactaggac tggaatggta aagagaatca
     6121 tccagaaaat taacaaaaag tccagcggaa ccgtgcgtct ccattcctta tgtttctttg
     6181 taaattggac aatgacacga gccaacatga ggatatagga tatcttcata aattctgacg
     6241 gttggaatag ggtaattcca tttattgata cccagttttt ggcacccgtt gatgcaacta
     6301 agcttggatt ataaaataca atcggcaaga tcataagtcc caagcctaaa atatatagaa
     6361 agggggtcac cttccaaaga aattctgtat taaagagcat gaccacaaaa ccaatcacaa
     6421 gccccaaggc aatccaggcg acctgctgcc ctaaaatggg cagaatatta ttggggtaat
     6481 catgactaac ggctatatag atagccacca caccgatgac cagtagaaaa aatactggca
     6541 agagcaaact gtaatcgact cttgagtcga gagaacgttt catatatact aaccttatac
     6601 tttcatacaa tactatttat caaagttcat taaaaaatct atcaatagcc tcgtcaactt
     6661 cggatcgaga gatggtttta acagtcgctt cttctgctag agatgctact atttgtttgc
     6721 cgtatcgttt tccgacgatt ctcctatcca aaataagagt taaggaacgt tggtattcac
     6781 gtctcatact tcttcccaaa gcctgtttta aacgaataat ggccattggc aattgataat
     6841 cataaaaggc atttttccct tcttgattca gttcttgatt aatctttttc gtcaagggtt
     6901 cttgaggatt ttggaaagga agcctcggta caacttgaat cacaaaagga tggcttgaaa
     6961 aatcaactcc ctcccagaaa cttgctgcac caagcaagat ttgttgttca cctttttcaa
     7021 agcgtttctt tagctgatga acatccccat ttttatactg ggccaagtgg ctaactgtaa
     7081 gtaaatccga tactgctaga agcatgtctt tagcggtaaa gagaaccaaa atcggttgct
     7141 ggaaagcttg aatttccact agtaaagcag ctacctctct ggcatagact tctaaggagg
     7201 tttctgttac cagagggaaa tctttgacca tgaccacttc ttgttcctgt tttccccgag
     7261 attcaatctt gacaaattta gcttcaggat agcctaaaag gtctgccaaa gaaaccctct
     7321 gactaatctc aagagtagcc gatactccca ggacttggca actctcagga agtaaagagg
     7381 acagaagagt acggcctgat tttgtagaag aaatctgaat tttcttttga ctcgtttcag
     7441 ttacttcaag ccagtaatca ccttcagctg taaaatagcg aaccagctca tcaaagcctt
     7501 ctacttctaa ttctgaaaaa tactgatgga gattatccag agaatctaag atatttttcc
     7561 tagatttgcc agactgaaat tgttctatca agtagagaca ctcaaagcga atactttcta
     7621 gtatccgttg ttgaaccctg ttttcttctc ctactaaagc cttatcaatt aaatcgataa
     7681 tagattgtat atcgtaggtc tcttgaagca gattttctag agctaacaaa atcttttgga
     7741 cttcatcaat aatcagtaaa cggtcactga caaattcagg attatcttca agtctggtca
     7801 cgagataggc atgattagtc actaaaagct tgcaagtctc tgccctttct tgactacgtt
     7861 tccaaaaatc ttccgtcaca aataagctat gggatgataa attcccatca tgacgaaggt
     7921 ctgctagaaa atgttggtaa cggtagagtt gcccgatttc atccaaatct cctgtctctg
     7981 tctcagtaag ccagaccaag acttgcattt taaagcgtct aaataagcga ttttcatcat
     8041 tttcctgcaa ggaatgataa aaggcatcca acttcagata attttgtggt ccctttaagc
     8101 tatgaatatc tgtatggaac acttccttga gacgtttacc ttcttcttcc atgatttgat
     8161 tttgaagaat ctttgtcgga acactaagaa caatttgtcg ctctttggat tgagataaag
     8221 cgggtaagag atagccatag gttttcccaa tccctgtcgg cgcttgaatc agagagacag
     8281 ggtcatcttt caatagcaag ccaacctctt tagcaaaact ttcttgttcc tccctcactt
     8341 caaggttcaa cagagaaata tttttagaaa agtcttgaga tagttttcgt agctccagag
     8401 aagctgccgt tttcttaaaa tacagacctt gaacttggac caagtctgga gaactcagga
     8461 tagattggtt gcgataagtt tcctcaataa ccaggtagga ctcatatagg agagcgtcag
     8521 ccatttccag caagcgttcc aagagacctt taggaagctg ggtcatcttt tcccgtaaaa
     8581 aaagaagtaa ttctgctgta gcttgggcat ctgaaagggc tgtgtgtgcg tgtttaagag
     8641 gaattcctaa ttctcgacac aaaatcggca agctatattt ttccagttca gggaaaaaga
     8701 cctgggccaa ttcgaccgta tcaacacgag ggtttcttag ctcatagcct tcaaaaaata
     8761 aattttccgc caagagatta gcatcaaact gaacattatg ggctacaaaa atcccatcct
     8821 ccaccaagtc aaagattttt ctggcaactt gcgaaaaatc aggtgcttgc gccagacgtt
     8881 ggtctgtcaa tcctgtcagt tctttgatat gagcatccaa gggttcatgt ggattgacat
     8941 ccgtcgtata gtgatcgacg atttctccat cctcaatcac gacaattccc acttggataa
     9001 ttttagcctt actacctgtg ctagttgcct ctaaatccac cacaacatag cgattaccaa
     9061 tcgttttcat tataaaaaat tataccaaaa aaagggccat ttggcacctg caaacatagg
     9121 ataattttca aactcaagaa cactactttt cttagaaatc ccatcgaata gagcattcta
     9181 gcctgctgat ttttttaaaa tagtgaagaa atacgaaaaa ttgagagaag aaatgttttc
     9241 atcttccttc tctcaacgaa tcatgtattt tatttaacca tgtcaggatc gtagtcataa
     9301 aatcctgtat caaggaaacg gtttttaggg tgaagtttac gaacttcttc atccagcaag
     9361 aaggcttggt catggctaaa gttgccctct tgaatcaagc tacgcgcttg gataaagagt
     9421 tttgcaatct caacaaagtt ctgaccagga gtgtagagcc cttctagttt ccagcgagta
     9481 aagccatgct ccaccaattc tgttaatttg atcatcaaat caaggtcatt gttggcaaag
     9541 atatgggtcc catgattatc ttcaaaaatg gaatagtggc tctctggatc acttggctca
     9601 gccaagaaga ggtcacgttt atgcgtcttt tcatcatcga tatgtgtaaa gttatagtag
     9661 ttttgcaaga gtggacgttt agaatgatgg atgacgctag caccgtaaac caaaacttca
     9721 gcaggaattt ccaaaatctc tggcattttg aaaagttcag ctgatggaat ttcacgcgcc
     9781 aaaacagcct cagatgcgcc agccttttgt ccccagaagt taatctgacg actgctagtt
     9841 accatggttg aagcatcgta gatggtctta aatgaataac catcgcggtt aactacgtaa
     9901 aagacgcctg catccccaat cgtaatatag tctgtcttga tttcttccaa gaagtctaag
     9961 aaaggcttga tacggtccat catatcttgg tgcatgagag cattgaccgc aacgagcaat
    10021 tccttaccag catcatgaac caacttagcg atttcacgta attggtcata actaaaggtc
    10081 gttggcagac gaaggccaaa atctttctca ccgacataga tacggtctac gccagcttcg
    10141 agtagttgtt caacttgttc aatactttca gcagttgctg taatgacaat cttttccata
    10201 agaaaaatta taccacattt ctccaaaatc atccatgctt tgaaaatgaa aagggacttt
    10261 attctttttg taacctttct gtaataattt tctgccgaaa aaatgataaa ataggtatgt
    10321 actgttaagg agagaatcat gcccgtaaga aaattacaat cctatgaggt agactatcaa
    10381 gaagaattaa accagcagct tcctcattac caagcttata cacctgaagc gcaatctgat
    10441 gccaatctca aggaaatcct attttttatt aatatcgctg ttttttgtat ctgtattgct
    10501 atctttagtt ttatcttttt agcattaaaa ttatcaactg ctcttgcctt tgccgcagca
    10561 atcggattca gcttacttgt tttaaaagtt caacggtcta ttatcaaacg aaaacgtaga
    10621 agataaatcc aaaatatcgc cccagttgga gcgatatttt tattttttct ttttagatgg
    10681 tgtctgaatg gcaatcttca cttcaaagat tgttcccttg ggtttattat ctttgacagt
    10741 aacagttcct tttagagcat ctacaatttg cttggctagg gataatccta aaccaaaacc
    10801 acctttttgc cgggttctag ctttgtctac tcgataaaaa cggtcaaaaa ttttcttttt
    10861 atcttctgtc gaaataccga ttccattatc agaaacaagt aaataaagat tgcgatcggt
    10921 cgccgagata agaaaatcaa tttcaccatc ctcctcagta tacttgacgg cattatcgaa
    10981 aagaatggtc atcagttgtt tcagaagaag ctgatctgtg acaattgttc gatggatacg
    11041 attttcaaaa cggaagacac gattattttc cgaagcaatc atctcgtagt ttgtgaaagt
    11101 tgtattaaaa aagctagttg gaacttctgc aagctccggc ttaatcccat catctctccg
    11161 agctaagttc agcaagcttg tcgttaaaaa acgcatattt cggacttctt ccaaactcga
    11221 tgcaatgctt tcgctcacat ccataatggt agcttctggc ttacgaaaaa gggtctctaa
    11281 gcgattttgc aaaactgcga gtggagttcg taactcatga ctggcatttt ccacaaaaga
    11341 ctgttgcttc tgcatactct caagcagggg cctaacactg accctagcta gatagagact
    11401 ggcaagtaaa gacaaaatcc agaaactagc catcacgacc acaatcaatt gctcatgctt
    11461 ttgactagcc tgctccaact gactggtatt aatcaagaca gcagcatact tgatattggt
    11521 tgaaaccgaa ctaatattgg tctccatcaa aatcacacga tagatttctt cctgcccata
    11581 gctattaaaa acctgaatct ggtagatatg tcctagttct ttcttttcta acttaatctt
    11641 atccaagccc aaaaatcgat ttccagaaag aagttgagta aagtctttat caaagagaat
    11701 gacttccgta ttggaactga cattaggttt tatttctact ttactagcgt ccgccctagc
    11761 attttctaaa tctttaatct cttctgttgc cctatttata gccagctgaa taactgcttg
    11821 aggattttca ctcagtccat gaagcttatc gtccaccgaa gtataaagac tcgaatgcat
    11881 gacttgtaaa ataatcagag tcattgtaga aaaaatcagg gtgaagacac cgaagttgcg
    11941 gataaaataa ctaaagtcat ccgcatacca tgttttttta agtttactga acatctttta
    12001 aaagataccc aacactacgc aaggtttgca aattctctgc aaaagtggtt ccctttaatt
    12061 tcttacggac ttttgaaaca tagacttcga caaccgaaat cgttgtatca ctatcaaatc
    12121 cccatagacg gtcaaaaatc tgcgtcttag gcaaaatcac attttgattt tgaaggaaat
    12181 aaactagtaa atcgaactct ttccccagca attcgacagg agtatcttca actttaacgg
    12241 tattggttga taaattaacc acgatattcc cataagtcaa ggtgttttca ttaaacttcc
    12301 ctgaacgttt gagaagggcc tgaatccgca ttttaagttc ttctaggtag aaaggtttgg
    12361 tcagataatc atccgctccc agttcaaatc catgtccctt gtcatccaaa ctttccttgg
    12421 cagtcataat cagaactggt gtcgtaattc ccttttcacg caattctttt aagacttgga
    12481 aaccattttt ttctggcaac atcaaatcca gcaaaatcaa gtcatagaca ccactctcag
    12541 cttcgtagag accttcttct ccatcaaata cctgcataac atccgcaaaa tcgtctaaaa
    12601 agtcaaatac tgaatttgac agacctaggt catcctcaac caataagatt tttatcatga
    12661 gaaactcctc cttattaaaa ctattatacc aaatttgcct taaaaaaaac tcaactctct
    12721 gcattttaca tgagatagct gagttttctt tttattttag gcttatttat gcatttccgt
    12781 attgaagaac aactgcttcg actgcagctt tttcacggct aatcaagtca acacgcgctg
    12841 caatttcctt gattcccata ccgatgttac ggctaagagc aaggtcagaa agttgcggtt
    12901 caaagaactc cttgtattcc gccaagcgtt gctgagtctt aaatacatga gcaggaagga
    12961 taacaaagct atcaaagctc atatctcctc caagggctgc cttaatccaa gcccagtttt
    13021 cacgcgccca agaccaagct gttttctgag ttgcttgatg agctaggaat tggtaatacc
    13081 aagcagacaa gtcctgtggt ttgaccacaa atttgtcctt ccaagaagta atcaagtttt
    13141 ggatattatc cgcatctgta ctgtatgcaa gagctgctgt caactggcgt ttaaagacag
    13201 catctgttgc gtgagtataa gtatcaagat aaagtgctaa caagtcttta gtctcatgat
    13261 gtttcatctc attaatcaga acttgtgaac gaatagctgc tgggagttct gcaagattct
    13321 ccttgtgtgt tgcgaagatt tggctagcga cttgacgagc ttctgcatca tttgagcgaa
    13381 tcatcatcga aacagccagc tgacgaacca attcatcctc atctgattct ccgtctttag
    13441 cttcaaaacc aagacggtca tagttatgac gagccaattt agcaaccagt cctttgaagg
    13501 ctgtttcagc atccgttcct tcatcaataa agcgctcaag ggctgaaatc acttgagaaa
    13561 cagctgaaac caccagataa gactcttcct tagcaagttt atcaagaact ggaagcaagt
    13621 ctgcataaga aatgtgccct gcctcagcca acaaacgacg ttcttgaaca atttgcagtt
    13681 tgcttgtgtt atcaagtgtc tctagctcag caagaacagc ttctaacaag tctccttgat
    13741 agtcggtaat atagtgggca gtattttcag tgttgagacg aagagctcct tcattttcag
    13801 caagaagagc tgcgtagcca gggatttcga tactttcagt ttcgagtgta tcaggcaagc
    13861 ctttccagtt gctattgagg ggcaccaccc agagacggtt cttgtcttcg ttctcaccga
    13921 tgaagaattg tttttgtgaa atcttcaaga catcattttc aactttgaca gtaagaactg
    13981 ggtaaccagg ctgttccaac caagaatcca tgaaggctgc gacatcacgt cctgacgctt
    14041 gaccaagggc atcccaaagg tcactaccaa tggtgttgct gtattggtgt ttttcaaagt
    14101 aggcatgcaa acctttagca aaatcagcat ctcctagcca acggcgaagc atgtgcatga
    14161 gacggcttcc tttggcatag acgatagcgc cgtcaaagag tgtattgatt tcatctggat
    14221 gtttaacttc gacgtggaca gactgaacgc catcagtagc gtcacgttca agagcaagag
    14281 gtactccacc tgtttggaaa tcttcaaaga tattccagct tggttcgatg gtatccacac
    14341 agacgtattc catcatatta gcgaaacttt cattgagcca aaggtcatcc caccatttca
    14401 tagtcacgag gttcccaaac cattggtgag ccaattcatg ggccacaaca agggcaactt
    14461 gttgacggct agcaaatgta gagttctcat cgacaaccaa gtaaacttca cggtaggtca
    14521 caagacccca gttttccata gcaccagctg agaagtcagg aagggcgatg tggagagatt
    14581 gaggaattgg atacttaact ccatagtaat cttcgtaaaa ctcgatagag cgaacagcga
    14641 tatccagtga gaaatcaaga tttgaaagtg gatgtgcttt ggttgagtag acacctacca
    14701 gggtaccatt tttagtttta gcggtcaccc cttgcaaatc accagcaaca aaggccaaca
    14761 agtaagaaga catgcgaggt gttgtctcaa acttccagat acctgtttcc ttacggtttt
    14821 caacatcgat ttctggcatg tttgacaagg ccaattcacc ttctgcttgg tcaaagcgaa
    14881 gagagaggtc aaaagttgct ttggcttcag gctcatccac acatgggaaa gcttcgcgcg
    14941 caaaatggct ctcgaactga gtagacaaga cctccttctt gactccatca actgtataat
    15001 aagaagggta aatccctgtc atgttgtctg taattttacc agaaaaggca agaaccaatt
    15061 caacttgacc agcctcagcc aattcgatat gaagggcttc attgtcatgg tcaactgtaa
    15121 atggacgagc ttgacctgca acttctacag aggtgatttc caagtctttt tggtggaggg
    15181 agatgcggtc actctgtgct tgaccagtga tggtcacttt cccagaaaaa gtcttggtct
    15241 cacgactcaa atctaaaaat aaatcataat gttcaggaac aaattgctta ataaaatgtt
    15301 caactgcttg catagttttc tcctattcta agtttaagag ctgctgctct tcttcaaaca
    15361 aacttattat atcatatttt gcttgaaaaa aaaggattgg acagcgattt ccaaaacttt
    15421 cttccttcta atagtctaca gtgggtagac cttgcgaaat tcttctaaaa caaccttgct
    15481 ttcagatgta aaagtcagcc ctacttcacc caaacagtct gtgaaaaagt cctcatgaac
    15541 ctggcgccaa taggcaagtg atttgtctcc ctcaccttcc ttaaaggcat gttgcgcaga
    15601 aacttgattg aagagttcaa cagaaacctt tgtaatttcg acaatgcaga cagactgatt
    15661 ttgactatct aaaatgatat cgaaggtccc tacttgggga agtggttcgt cttctagtac
    15721 atagaggtca taggctgatg ctgttgctgt cttttctcct ttaaacacca aatccgctaa
    15781 aaggtctggt tcaactccaa aagcccaagc atcgatttca tctccgatca aaggattgat
    15841 ttgcttgtat ttattccaca tttcttgcgg tatcatgggt actcctttgt aattttttac
    15901 tttcttcttt tatgtgttta agatgatctg gatggtcaat ctctaaatca aaaatctctg
    15961 gaatagaact gtagtggata atgcacttga tacccaactg attcattttt tgtatgaaag
    16021 aagtattcag atagcctgct acagcaaaat caatcttgtt ctttcttgct ttatcctgca
    16081 tatctcttag catatctaac attattggac tttccatatc atgccattga ctgtttctca
    16141 tagtcgcaaa aacaaaggaa gtcaaatcat tcattccaac tacaatcttt gaaatgcccg
    16201 tttccagtat actagataag tcaaaatacg ctgacggtaa ttcaatcatc gttccgactt
    16261 tcccagtaaa accctgctga cgcaatactg taatagcttg ttttaattgg tcgtcatcat
    16321 tgacaaaagg aaagataaca gatagattgg ggttggtttg ataaacttct gtaacgacat
    16381 gtgcttcagc ctgaaattca tccaaacacg ccagtaaacg cctggttcct ctatagccaa
    16441 acaagggatg cccttcgtca aaaaactctc tagtccccac taaacaattg gcttctgtat
    16501 tcgttaattc agaaaaacga taccaaactt cttcatctga gtacagggag cagatagtct
    16561 ctagataatc ttttacaaat tgctgacaac ttgctaatat gatgttttga ttaagctctc
    16621 tcaacaaata ttccccacga atcatgccga cgtggtgaaa tagttgagga taaatttttt
    16681 caagaatttt ttcgccacta agggcaagtt gatttctcat cattcacctt ccaattcatg
    16741 taagaagtct tgtccagttc tggaaatcct aataattcag acttaacctt caagactaat
    16801 ggcgatgcat tttcttctgt aatttcttga atatccatcc aaatatatcc aagtgaatca
    16861 ttcgcaccat cagacacagc ttccgaagtc gtaacttgag gtgcactctc attcatttca
    16921 acatcataca aggccatgac atggtgaacc ataaaatttt ttaactcttc tctgacgaaa
    16981 acatcgtaga ttcgaggatt agagtagctt ctaacagtaa atctcgtctc ttccataact
    17041 tctctagtca gcgtttccgt cagtccttca ccaagttgct gactgcctcc aggtagatca
    17101 taccgatgtt gataagggcc tctcgttttt tcaatgcaaa gtaactttcc attttcaaag
    17161 caaacacagt agaccccaaa gtgttttttg atttccatcc aactcctcct acttcaaaga
    17221 ccagccacca tctattgtca agatttgtcc ttgcatggcg ctcgcttttc cacttgctaa
    17281 aaaaagacta agctctgcta tttcctctgg ctcaatccag cgcttgattg gggtttcact
    17341 agccacccag tcagccaaac cacctggttc aaaatccgca gcggtcatag ctgtcttgac
    17401 tgctcctgga gcgataccaa agacctgaat cccagcttca gcatagtcta gagccaactg
    17461 cttggtgaag ccagccaagg catgcttgga tgaagtatag gcgtgaccac ctccacctgc
    17521 taggctagaa gcaatggaac acatattgat gatgattccc tttttatttt ccagcatttg
    17581 tgtcaaataa taccgagtca actctactgg aataatgtag ttgatttcaa aaatctcttg
    17641 aatgtcctgc gccgtttgtt ccaacagtgg tttgtaatca tccaaaactc cggcagtatt
    17701 acacaaaaca tccacctgag ggcaccagtc aaaaataggt tccaagtcca aggtcaaatc
    17761 tctctgtaaa aagtgaaaat caccctctaa gagtggcttt tcaccttggt caactccata
    17821 aacttgatag cccttctcta aaaagaggcg agcttgagcc aatccgatcc ctgaactcac
    17881 tcctgtaatg agtacacgtt tagtcatgca cttctaccca atccgttgcc aaaacatcac
    17941 aagctgtcgg gctccacatg gaaaaacctt ctccttctcc agacacgttg attaggaaat
    18001 aaggtgtcac ttcaagtgca agcccatttt gctcgatggt atcaaagagt tggacatagt
    18061 tttccgcacc tccccaacta gttcgtacat attttttctt agtctttaag ccaggtaaaa
    18121 tttcttcaaa tgtcatgttt ttctccttta atgtcaataa ttctcccctt cattataaca
    18181 aaaaaaccgc tttaaaacgg ctttttgact gtgatttatt caaacctgct actacttacg
    18241 gcaaattatt ccctgcagca agataaattt cataccattc ttttcttgtt aagctaaagt
    18301 ttgccgctcg gctaacttct ctcaagtgct taggatttgt tgtacctacg actgcctgca
    18361 tttttgctgg ataacgcaat atccaagaaa tggcaatagt tgaagaggtt actccatatt
    18421 taatagctaa acgatcaagt acttgattta aagcttgaaa tttctcattt ccaacaaagt
    18481 tccctttaaa atacccgaat tgcaagacag accatgcttg aatgaccaca tcgtgtaatt
    18541 tgcaatattc aaaaatgctg ccatctcgca tagctgcttg actatcttcc atattaacat
    18601 gaaaagctga ttcaaatcct ggagtaaaag ccgcactcaa ttgtagctga ttaacagcta
    18661 acggctgctt gacatctttt ttaagcaact ccatcatcat aggattttga ttagaaactc
    18721 caaaatctcg aactttacct tgtttataaa ggagatcaaa ggcttctgct acttggtcag
    18781 attccatcaa agcatctggt cgatgaagga gcaagctatc tagatgatca atcttcaatc
    18841 tttgcaaaat accgtctact gattttataa tatagtcctt agaaaaatca aaataggtaa
    18901 attcttcaat gcgaatgcca catttggact gaatccacat cttttctctt aaatctggac
    18961 gattttttag gacaagacct aacagttctt cacaacgacc acgaccataa atatcagcca
    19021 agtcgaaggc attgattcca acagaaagtg ctgtttctac aagctcttca acttctttta
    19081 cagacttatc ttttattctc atcattccga gaacaatttc tgataattct ttgtcatctt
    19141 gaccaagagt tatgtatctc atcaaatttt tctcctttaa tttctaacat tcttcccttc
    19201 attataacaa aaaaccgctt tgcaacggct ttttgactat acttcactcc attttatctt
    19261 cttaaaccca cggaacaaga caaagattcc aataaagagg acagctaaag gaataacttt
    19321 tgtaaggaaa acatttgaaa ttcccatcca ctcataataa cggagcagag aacccaccac
    19381 aagatgggca ataatcatac tgacaaatgg acgaaagacc gcttctttcc aattccaaat
    19441 accgataact agcgaaatcg taaagacaga caaactatcc cagggagccg gaatataaaa
    19501 ggctccttct tgtatgaagc ttgccattcc tacatatcct aaaacaacta gaagaactat
    19561 agtcccaaca acaatgtaag tgccaatttt cattttagga gaatcttgga ctaaacttct
    19621 tcgtaaaatt gtggccacaa gtccaaatcc aatcagaaaa ataagaagtt gccctaaaaa
    19681 tgtgagcaaa ttgactgtta agagaggacc tttagaaaaa tcacttagta gttgataata
    19741 acgtaatacc gccaggacaa gaattggcgt caaaagggac tctttgatag aactgcgagg
    19801 tgctcccttg agaatctctt tcattatttt tttaggattc ttacctagat aatcctctgc
    19861 actcatgcca tctcgttctg cttctgagaa atctagcatc atcaaataga tctgctctct
    19921 gagatagtct tcatcataga gaaatccagc aagattaaaa ctttcccaca actcctcaaa
    19981 atacttttga ttctcctcag aaaactcatg tagcaaagcg cttgtttctt cgtaatactt
    20041 cattttcttc atggtttaac ccccattctt aatcccttct actttttgac tcaaatcgtc
    20101 ccattgttgc caaaagactg agacacgctc ttctccttct ttcattaatg aaaaatactt
    20161 ccgatctgga ccatctggcg acgggcgcat gtcgcctctt atccattgat ttttttctaa
    20221 cttttgcaac aaaggataaa tagttcctgg aacgatagta tcaaatccag cctctcgcaa
    20281 agtctgaacc aactcataac cataccgctc tttttgacca atcatatcca agacacaacc
    20341 ttcaagaaca ccttttaata gctgagtttc tttcatcact tctcccttct aatctatttt
    20401 gtaataccta ctagtaactt cacctatagt atatcacttc tacactagtt tgtaaagcat
    20461 aatagttaat actcttcgaa aatctcttca aaccacgtca gcgtcgccct accgtatgta
    20521 gggttactga cttcgtcagt ttcatctaca acctcaaaac catgttttga gctgacttcg
    20581 tcagtttcat ctacaacctc aaaactgtgt tttgagcaac ctgcggctag cttcctagtt
    20641 tgctctttga ttttcattga gtataaataa aaaaacagaa ctagcctgaa ctagtcctgt
    20701 ctacttttac ccaatcacac ttccatttgg tacagctgga tcaactgtga gaagggttaa
    20761 tttgccatca tgttcagctg agagaatcat accctggctg acatattttt tcatcatttt
    20821 acgtggtttg aggttagcaa cgatttgaac tttcttgccg accaattctt gttcatttgg
    20881 atagtatttg gcaattcctg aaagaatctg acgatcttct ccatcaccag catccaagca
    20941 gaattgaagc aacttatctg aaccttctac tttagacact tctttgactt ctgccacacg
    21001 gatttcaacc ttgtcaaagt cttcaaactt gatttcatcc ttgtttagtt tgagctcaac
    21061 ttcgtccgga ttccattctt tttcgactgc tggtttattg ccttccattt gttccttgat
    21121 ataggcgatt tcttcttcca tatttagacg tggaaagata ggtgttcctt tggcaactac
    21181 agtcacatct gctgggaagt cagccaaact caagttttca agactagaaa cttcttccaa
    21241 accaagttga gtcaaaactg cacgactagt ttccatcata aatggttcaa tcaagtgagc
    21301 aactacacga atgctggctg ccaagtggct catgacactt gccaattggt cacgaagagc
    21361 ttcatccttg gccaagaccc atggtgcggt ctcatcgatg tatttattgg tacgagagat
    21421 cagagtccag actgcttcaa gcgcacgtgg atagtcaact gcttccatgt gtgtatggaa
    21481 gtctgcgatt gattgttctg caacctcagc aagaacatga tcaaattcag tcacaccttc
    21541 tacataggca gggatttgtc catcaaagta cttattaatc atggaaaccg tacggttaag
    21601 gaggttccca aggtcattag ccaattcata gttgatacgg ccaacatagt cttcaggagt
    21661 aaaggttccg tctgaaccaa ctggaaggtt acgcatgagg tagtaacgaa gtggatctag
    21721 tccataacgc tctaccaaca tttcagggta aacgacattc ccttttgact tagacatttt
    21781 tccgtctttc atgacaaacc aaccatgggc aatcaaacga tcaggtaatt taacatccaa
    21841 catcataaga aggattggcc agtagataga gtggaagcga aggatatctt ttcctaccat
    21901 atggaagact gttccattcc agaacttgtc aaagttacca tgttcgtctt gagcgtagcc
    21961 aagagctgtc gcatagttaa gaagggcatc aatccaaacg tagacaacgt gttttggatt
    22021 tgatgggaca ggcactcccc atgtaaaggt tgtacgagat accgccaaat cttccaaacc
    22081 tggctcgatg aagttgcgta gcatttcatt aaggcgacca tctggcgtga taaattcagg
    22141 atgagctttg aaaaattcga ccaaacggtc ttggtatttg ctaaggcgaa gaaagtatga
    22201 ttcttcagaa acccattcaa cctcatgacc tgatggagca ataccaccag tcacatttcc
    22261 agcttcatca cggaaaactt ctgccagctg gctttctgta aagaattctt cgtctgatac
    22321 tgaataccaa ccagagtatt cacccaagta aatatcatct tgagcaagta agcgttcaaa
    22381 gacctgtgcg acaacttttt catggtagtc atcagttgta cggataaatt tatcgtatga
    22441 gatatctagt aattgccaga gttctttaac tccaaccgcc attccatcaa cataggcttg
    22501 aggtgtaata ccagcttctt ccgctttctg ctggattttc tgaccatgtt catcaagacc
    22561 tgtcagataa aatacatcgt agcccatcag gcgtttgtaa cgtgctagga catcacatgc
    22621 gatagttgtg taggcagaac cgatatgaag tttcccagat ggatagtaaa tcggcgttgt
    22681 aatataaaaa tttttttcag acataatttt tcctttccag gcaaatgaaa cctgtttttc
    22741 taacacttca ttatatcaca tttttaatga atttcgatag ggaaatccat acaaaaacaa
    22801 gatagacgag tgtccatctt gttgatctca ttcataacga agggcttcaa ttggatcaag
    22861 tttcgatgcc ttgttggctg gcaagactcc aaaaatcata ccaacactag ccgaaactgc
    22921 aagactaaat agggcaactg ggattgatac tccaacttct ataccttcta ttaaaccttg
    22981 cagtaacaaa cctgctaagg cagttaaacc acttgcaatt gtcaagccaa ttaagccacc
    23041 taacaaggtc aaaatcatgg attcaatcaa aaactgaatt aaaatattgg cacgtgttgc
    23101 acccaaagcc ttacgaagac caatctcacg agtgcgctct gtcaccgaaa ccagcatgat
    23161 gttcatgaca ccagttcctc caacaaagag agaaatccct gcgatggaac taataatcgt
    23221 cgtcataaaa ctaaacgatt gttgaatttc tgcaaataca acggactcat ctgccacctg
    23281 gtattctccc tgttgtaagc ctgcaagctc tgtcattttt cgtgccagtt ctggacccag
    23341 agttggggtt aaactggtat cattcactcg aaagacaata ttagctattt catctacatt
    23401 aaaattcgca gcaagggaga tattggtagt aataggcaag ccaccaaacc catatatttt
    23461 tgacctttta gcctccggac tagtataaac cccaatgacc cggtaactaa atccattgac
    23521 ttctacaacc ttgttaatag cctcttgagg agattcaaat aaactaatgg acaattcctc
    23581 atctagcaaa atgacacttg caaactcttt gaaatcttgc tctctcagac tacgacctgc
    23641 aataatttca ttcttaacag cgtccatgta agttctgttt ccacctgtca aattagcatt
    23701 ctcaaccttt ttatcttgat aggtcaagat ggcattcgtt gaattggtta catagtaact
    23761 atccactccc ttcagtttag ctgcctcttg gacccaggat tcttgcggtt ttggcggttc
    23821 aacaggaact tcctcttcct ttccagaaac cgtaaaagct gattgtttct gagtaaaaga
    23881 cccatcttta ctttttttag gagagaaaaa gacgctaata tttttctgag atttagtcat
    23941 atctttattg acttgacgag atagggaatc acccaaagcc ataatcacaa caactgatga
    24001 aacaccgata ataatcccaa tcatagtaag caaagaacgc atcttgtgag ccatgataga
    24061 tgaaaaggca aatttcagat tctgcatctt agttttcctc ctttcctaac tgagcactgt
    24121 cagacgaaat gaccccatcc cgaatgacaa tctgacgttt ggcataggca gcaatctcag
    24181 gctcatgcgt taccatgata atggtttttc cttctttatt caaatcaacc aataattgca
    24241 taatttggtt acctgttttg gtatccaagg ctcctgtcgg ttcatccgct aggataatag
    24301 aaggattgtt taccaaggca cgcgcaatgg ctacacgttg cttttgacca ccagataatt
    24361 ctgaaggtaa atggtgacta cgttctgtca attcaacctt gtctaaatat tcctcagcca
    24421 acttgcgacg ttttgaagac gaaactcctg cgtaaatcaa gggcaattct acattttgca
    24481 gagcattgag cttcgataga agaaagaact gctgaaagac aaaaccgatt tgttggttac
    24541 ggaccttagc tagttgtttt tcaccaagcc cagccacttc ttgaccttca agataatatt
    24601 ctccactggt tggtgtatcc aacatgccaa tcgtattcat cagagtggac ttaccagacc
    24661 cagatggtcc catgatggct acaaattcac cctcattcac ttctagattg atatttttga
    24721 gaacctgcag ttcttggtca ccattacggt aacttctgaa gatatttttt agactaatta
    24781 gttgcttcat cagccttcac ctcttttcct tcttccaagg aagatgttgg attactgatg
    24841 accttagcac cgttcgttaa accagaagtg atttcttgat tttctgcgtc agcatttccc
    24901 aatgaaacct caactttttt agccttttgt tgttcatcca caatccagac ataattttta
    24961 ctatcatcca ttactagact gctaacagga acaagaatag ccttagtttt gcttttaacc
    25021 tcaatgttga cagaaaaacc ttgtttcaaa tcaccaacct cgcctgtcac atcaatagta
    25081 taagggtatt tagaacctgt attattcccg gctgctggac tagctgcttc accattgttt
    25141 ttaggatagt cagaaatata gcttaatttc ccagtccatt ttttatcagg atacacttta
    25201 gaagtaaagc ttacttcttg acctacagaa aggttggcta gattgtactc agacaattct
    25261 cccttgactt gtaaattttc attgctgaca atatgaacca taacttgact cgcccctgtt
    25321 ggagatttag aaacattgct attgacttcg actacagttc cctctagggt actgagaaca
    25381 gttgttgcat ccaattgact ttgagccttg cttaattgcg ctgcagcatc tgcacgcgca
    25441 tcacgggcat cacccaattg agcatcaata gaagcaacag aatttccagc cactggagtt
    25501 gggctttgca ccgttgcatc ttctcctcct actggcgctg gtaactgtgg agcctgagct
    25561 gaagcggctt catttcgtgc ttgattgagt tcattgatat gacgatctgc cttagctact
    25621 gctcgactag ctgaatcata ggccgcctgc gcttctgaac tactgtactt gactaaagcc
    25681 tgcccttcgc tgaccttatc gcccacagaa acaaggattt catctaaatc acccttacta
    25741 gcatcaaaat aaacatattg ttcatttttt gctgttactg tccctgacaa taaaacagag
    25801 gatgccacgc ttccttcatt ggcaacaaca agatgagtag gctcatcttt tagagcagtc
    25861 tgagaaggtt gtctaaagag taaaatcccc ccggcaccca atacaactac actcgcagca
    25921 ccgattgctg catacagttg ccacttttta gctttaccat tctttttctt cataatgaaa
    25981 ctccttttct tttttacaat actttgctat tataccaaat ttccctccag caaacaatgc
    26041 agttcagatg gaaataatgg cagttcagga ttaaacaatc gttcggaatt ttgcttttct
    26101 attgtactag atatataata cactatcttg acttatttgg caagcttttt tgcaattttt
    26161 tgtgcgtagc agatttttgc aatcaaatca aaaaaagaat aaaatataac tatcaaaaaa
    26221 gacactctac tatttaaaag attacaaagg agttttcaat gagagaatat gatatcattg
    26281 ctatcggtgg aggtagcgga ggaatcgcta ccatgaaccg tgctggtgaa catggagcca
    26341 aagcagccgt tattgaggaa aagaaattag gtggaacctg tgtcaacgtc ggttgtgttc
    26401 ctaaaaaaat catgtggtac ggggcgcaaa tcgctgagac tttccatcaa tttggagaag
    26461 actacggctt taagactact gatcttaact ttaactttgc aaccctacgt cgcaatcgtg
    26521 aagcctacat tgatcgcgct cgttcttctt atgatggtag ttttaaacgc aacggtgtag
    26581 acttgattga aggtcatgct gaatttgtag attctcatac tgtaagcgta aatggtgaac
    26641 tgattcgtgc taaacatatc gtgattgcta caggtgccca tccaagtatt cctaatattc
    26701 ctggtgctga gctaggtggc tcttctgatg atgtatttgc ctgggaagaa cttccagagt
    26761 cagttgccat tctaggcgct ggttatatcg ccgttgaatt agctggcgta ctccacactt
    26821 ttggtgtcaa gacagatctc tttgttcgcc gcgatcgtcc tttacgtggt tttgattcct
    26881 acatcgttga aggtttggtc aaggaaatgg aaagaacaaa cttaccactt cacactcaca
    26941 aagtccctgt caagttagaa aaaactactg acggcattac cattcatttc gaagatggta
    27001 ctagtcacac agctagccaa gttatctggg ctacaggccg ccgtccaaac gttaagggct
    27061 tgcaacttga aaaagctgga gtgactctga acgaacgtgg ctttatccaa gtggatgaat
    27121 accaaaatac tgttgttgag ggaatctatg ctctaggtga tgtaacgggc gagaaagaac
    27181 tgactccagt tgcaatcaag gccggacgta ccctatctga acgtctcttt aacggaaaaa
    27241 ctactgcaaa aatggattac tcaactattc caactgttgt cttttcacac cctgctatcg
    27301 gaactgttgg tttgacagaa gagcaagcta ttaaagaata cggtcaagac caaatcaagg
    27361 tttacaaatc aagctttgca tctatgtact ctgcttgcac tcgcaaccgt caagaatccc
    27421 gtttcaaact cataacagct ggttcagaag aaaaagttgt cggacttcat ggaattggct
    27481 acggcgttga tgaaatgatt cagggatttg ccgttgctat caaaatggga gcaaccaagg
    27541 ctgactttga cgcaactgta gcgattcacc caacttcatc tgaagaattt gtaaccatgc
    27601 gttaatcgaa acaaaagaag ctgatataaa tatgtctgct tcttttttaa tgattttcga
    27661 atcaacaaaa aattatccaa cttgactagt cacgaaaatc ggctgttaca gtttttgtta
    27721 ccttgttttt aaaaaatggt tgacatcgac tcctatatga gtataatagt tactatacat
    27781 tagaaaagag gttaactatt ttgaaaaaag ctcacgttta tgctatccct gctattgggg
    27841 ctgctctcat tgctgttttg gcacaaatca gtcttccaat tggacctgtt cccttcactc
    27901 tgcaaaactt tgcaatcggc ttgattgcta ctgtctttag accgagagag gctgtacttt
    27961 ctgctggact ctatcttctt ctaggtgcta tcggtcttcc tgtctttgca ggaggtggag
    28021 ctggttttca ggctttagtt ggccctactg caggctatct ttggttttat ctcgtttact
    28081 ctggacttac ttcctctcta accaacagca agagtggtgt tgttaagatt tttcttgcaa
    28141 acctcttggg tgatgccctt gtctttgtcg gcgggattct cagcttgtat ttcctagctg
    28201 gaatggcatt tgaaaaagct cttgttgtgg gggttcttcc ctttatcatt ccagaccttg
    28261 gcaaacttct ggctattagt tttattagcc gtcccctact tcaacgcctt aaaaatcaga
    28321 cttactttac taactaaaaa aggataccga gttatcatga ctcaatatcc ttttttttat
    28381 tttgaaaact tatactcaat gaaaatcaaa gagcaaacta ggaagctagc cgcaggctgc
    28441 tcaaaacact gttttgaggt tgtagatgaa actgacgaag tcagtaacca tacatacggc
    28501 aaggcaaagc tgatgcggtt tgaagagatt ttcgaagagt attagttgcc tttaaaggca
    28561 tccaccatcg tttgaaattc ttcatttgag agagtaatcc ctttgcccat tttagtatgg
    28621 tctggactcc aagcacgaat atcaaacttt gcaggggcac cattaaagct cacacggtta
    28681 atttccttgg tccaaccttt ttcgttttca gaaagagtca acaagtgctc ttcgatttca
    28741 aatgtaaatt ctgccatttt cttctccttt tttagtttca ttagtttatt cgtaaaatct
    28801 tgtagatttt aggaaaattt tatataatat tgatataaaa gaagggaggc caatatgaga
    28861 cataaattcc agcaagttct agataaaata catgattttt taaatggaca tgaccaacct
    28921 gaccagactg aaaccaactc ccttacagcc actattgaag aggctatcca gaaacaaacc
    28981 gctgttcacc ttatcttgtc tgagacaagc tttacaggtg atatcatcaa atatgatcag
    29041 caaggccagc aaattatcgt gaaaaatttt tccaaaaatg tgagccggat tatccgtata
    29101 agcgatattc aacgcctgcg atttgtcccc tcaactgtcc aaacagccca aaaaaataga
    29161 tttaagaaag agtgagatgt agttgcttca tcccactctt tttcttagcg aatttgttca
    29221 aaatgtaaat gaactgcgat atgatctcca taaccacttc tttccaagtc acgttgtaaa
    29281 cgataggaaa tatagtgttc tgcaatggta atgtaacctg cgcccaataa acgatgttca
    29341 accatagatt gaatcatact gatagtcgca cgttccacct tggcttcttg taaatccaaa
    29401 actaccttct tagtgacttg agcaagattt tgacgcaaat catctgtcaa aacataaaca
    29461 gtttgggctg ccttcaagat ggcttggtaa atcttatctg gattaaattc agcaatttcg
    29521 ccattacgtt tgattacttg cataggtttc tcctttattc tttgttttct ttgatttctg
    29581 ccagcatttt ttcttcttct actgtcagtt gataatgttc aagtaaatcc ggtctgcgct
    29641 cgtaggtttt ctttaaactc tcgtacaatc gccactgacg aatcttttca tgatggccac
    29701 tcatcaatac atctggcacg accatgcctc gataatcata gggacgtgtg tactgaggat
    29761 attctaaaag acctgaagaa aaactatcat cttggtggct agactccttg ccaatcactt
    29821 ctggaatcag gcgaactgta gcatcaatca tggtcatagc tgccaattct ccaccagtga
    29881 ggacatagtc gcctagggaa atctcatctg ttaccaaggt cttaatgcgc tcatcataac
    29941 cctcatagtg cccacagata aagattagct cttcctcttg agccaaatct tcagcataag
    30001 cctgatcaaa ctgctttcca gcaggatcga ggagaataac gcgcggattt ttcttttcaa
    30061 tagcatcaaa ggaatcgaaa ataggttgtg ctctgagcaa catgccctga ccgcctccgt
    30121 agggctcatc atctacatga cgggcctttt cagcattttc tcgaaaatta tgatactgga
    30181 tatccaagag ccctttttct cgagcctttc caacgattga gtgctccagt ggagaaaaca
    30241 tctctggaaa gagggttaaa atatcaatct tcatcgtcta acccttctaa gatttccaca
    30301 tcgacccgtt tatttggaat atcaacattg agaaccactg gtgggatata aggtaaaagc
    30361 aaatcacgtt tgccttttcg tttgaccacc cagacatcat tagcacctgg ttgcaggatt
    30421 tccttgatgg ttccaaccaa gctatcaccc tcatagactt ccaaaccgat aatctcgtga
    30481 tagtaaaatt caccatcgtc taggtcattc aaatcttcct cagcgacctt gagactgtat
    30541 cccttgtact tttcgatagt attgatatgg tacatatctt tgaatttaat aatgtcaaag
    30601 ttcttctgtt tacggtggct agcgatggtc actgtttgga caaactgatc tttttcatca
    30661 aacaaagcca gctcagctcc ttttttaaac cgttcttctg caaaatccgt cacagacaag
    30721 actcgcatct caccctgtaa tccctgcgta ttaacgattt tcccaacatt aaagtagttc
    30781 atcttgtctc ctgtaatctc cttttttcca tcttattctg acaattctcg aataatagcc
    30841 gcaatttttt ccgattctga ccattgtaaa taatggtgat tccctcctaa aatgagttta
    30901 gtattggaag tccaatattc tgattctctg tactcttttt ctctataagt ctgacaaaaa
    30961 ataaatacag ggacatgaga ttctatcgac aaattctcaa aatcttcctc agtaatctct
    31021 ccagatatct gaaattctgg atcttgattt tccaactcta agcctttttc ttgcattaat
    31081 tcccagattt ttttattcgt ttcaggacta aatgttgctt gagttaagtt cttaaaataa
    31141 agttcaggac cacactcgtc aatcagcctc atctgctctt ccatttctgg ataaggattt
    31201 tctgaaaaat cagcaaacat gactttttta gttgtcggtt caattgctac taaagtctga
    31261 cacttaattg gtttctcgag taatttgcaa gctaaaattc cactccaact atgtacacaa
    31321 agtatatatt cagaaatccc taattcttca agtgcttcat aaaccacatc tgcaagatta
    31381 tctagatttt ttccagcttg gtcataaatc ggactcctac ctgtgttcgg aaaatcaatt
    31441 gtcaaataac caattgtagg aggaggtttt tcaagtataa gtgaaaaatt ttcataactt
    31501 ggtagcaaac ctgctccatt tagacaaact agcactttct tttgcttttg ataagcaaca
    31561 gagagactac caatttctgt agatacttca aacctcttca taaagaaatc cactcattct
    31621 atataatgaa ttattaaaaa tcattatcct ttattttatc acgttccaag gattttctca
    31681 agttggagga aggggacaac atctctactt tcccttcaag aatccttcca aattatgttt
    31741 atgttggtaa ttaacggctg cggttttgtc tttctcaaag acagtcttgg taaggtcaat
    31801 atgattaata gctacgattg cgacggtgta gtaaatgata tcagccagtt ctctggcaag
    31861 ttcctcgttc gaatcctatc ccttcttttc gaccagagcg cctattcaaa acctcggcta
    31921 cttttccgac ttcctccact aacttcataa agagaccttc atcagtccga gactgctgtt
    31981 aatgttcgat taagtagtct tggaattgcc taaacgttca atcttttata gtatattgaa
    32041 actagaatag tacaccttta cttctaaaac attgttagaa atcgatttga ctgtcctgat
    32101 cgatttgtcc tgttcttgtt tcattttact atatcttcta ttccacacaa aaaagcgaaa
    32161 catccgtccc gcccttctta tttttcgtca ataacgattc ttactttttt gtattcagtt
    32221 gggacagagt agacaatcgt tcttatcgca gaaatagtgc gacccttacg accgattaca
    32281 cgacccacat cgctttgatc aagattcaaa tgatattcca aaaattctgg tgtatcctca
    32341 atcttgatag ttaaggcatc tggttgtgaa attaagggtt tcacaatcgc aataatgaga
    32401 ttttcaatcg tatccatctg tcaacctact ttaaacttat tttgaaaatt tagaatcgtg
    32461 gaattttttc aatacgcctt cttttgaaag gatgttacgt actgtgtctg aaggttgagc
    32521 tccattagcc aaccatgcaa gaacgcggtc ttctttcaaa gttacttggt tttcagcaac
    32581 aagtgggttg taagttccaa ctgtttcgat gaaacgtccg tcacgtggtg aacgtgaatc
    32641 tgctacgttg atacggtaga aaggtttttt cttagaaccc atacgagtca aacggatttt
    32701 aactgccatt tttaaagtct catttcttta attttttatt tcggtgaaat agctgagcta
    32761 tttagcacat gttctattat agcagatttc tggcatgtgt caagaaaaaa acttgacaga
    32821 ctataaattt cttaaagtat ttagaaattt gttagaatag gaaataaatg tttgaaagat
    32881 gctatccgtg aatactattt tagaaaaatt ttataaagaa caccaagtca aacccttcat
    32941 atcgcctgaa cgagagttgg atacttggct tctaagtccc aagcccgttc ccaagcgaaa
    33001 tatggactta ttagtagatg attcactagc aggagatatc attttactat ggagaatcca
    33061 gtttggtact tttaccactg aaacatgatt ttaaaaactc ccattcgaat atgattcaaa
    33121 attcgcttag aatcaatgtt ttcccacttt ttcaaaaaat aaattttcat ctaatttaaa
    33181 taactttcaa ttaagtgtga attttgctta aaatcattaa aaaacacccc atggtgtgaa
    33241 ccatgaagtg ttgtaaatgc tgtgttgtag ctagttctta gcgtttagag aactggctag
    33301 ctttacgagc tttcttaaga tctggtttcc aaaatacttt gagtacactt atggtattga
    33361 tgcccctaaa catctaaaaa ctctggtaga aaaagggtat gctcttgtcg aaacttcttt
    33421 tgactcactt gaccatctaa atactaccat gaaaaagaat atcctgaaga agaagggagt
    33481 tactggactt tctaaaatga aatcttgcga cctggatcaa gcccttcatg agcatttttc
    33541 agaagaagaa ttagctggtc actttcatgt ccttctatgg acttttttac aatggcattg
    33601 ctatcacacc caatacctat ctaagcgcct ggttcgtaaa ctttattgca gctcttcctc
    33661 taaatttcct aattgttgaa ccaattgccc gttttatact aagttctttt cagaaaccat
    33721 ttactgggga agaagttgaa gattttcaag atgatgatga aatcccaact attatctaag
    33781 ccagttctgt aaactactaa tatttgaaat ccacttcctt ttagggtaca atggttataa
    33841 atgaattttt gagaggatca gaatgaaaaa actagcaacc cttcttttac tgtctactgt
    33901 agccctagct gggtgtagca gcgtccaacg cagtctgcgt ggtgatgatt atgttgattc
    33961 cagtcttgct gctgaagaaa gttccaaagt agctgcccaa tctgccaagg agttaaacga
    34021 tgctttaaca aacgaaaacg ccaatttccc acaactatct aaggaagttg ctgaagatga
    34081 agccgaagtg attctccaca caagccaagg tgatattcgc attaaactct tccctaaact
    34141 cgctcctcta gcggttgaaa atttcctcac tcacgccaaa gaaggctact ataacggtat
    34201 taccttccac cgtgtcatcg atggctttat ggtccaaact ggagatccaa aaggggacgg
    34261 tacaggtggt cagtccatct ggcatgacaa ggataagact aaagacaaag gaactggttt
    34321 caagaacgag attactcctt atttgtataa catccgcggt gctcttgcta tggctaatac
    34381 tggtcaacca aacaccaatg gcagccagtt cttcatcaac caaaactcta cagatacctc
    34441 ttctaaactc cctacaagca agtatccaca gaaaattatc gaagcctaca aagaaggtgg
    34501 aaaccctagt ctagatggca aacacccagt ctttggtcaa gtgattggcg gtatggatgt
    34561 tgtggataag attgctaaag ccgaaaaaga tgaaaaagac aagccaacta ctgctatcac
    34621 aatcgacagc atcgaagtgg tgaaagacta cgattttaaa tcttaaaaac caaaaaaaat
    34681 acagtatcca cattcggtac tgtatttctt ttactctcat tcttaagtta aattattaaa
    34741 atcccatatt tggtctatcc agccttcata aaagtctggc tcgtggcaga ccataaggat
    34801 agatccccta tattctttga gagcgcgttt gagctcatcc tttgcatcca catccaaatg
    34861 gttggtcggc tcgtccagca ctaaaacgtt gttttcacga ttcatcaaga gacagaaacg
    34921 aaccttggct tgctctcccc ctgataatac ttgaatctgg ctttcaatat gtttggttgt
    34981 caaaccacaa cgggcaaggg ctgcacggac ttctgcttga ttaagggcag gaaaggcatt
    35041 ccagacagct tcaagaggag tttggcgatt accaccttct acttcctgct caaaataacc
    35101 aagttctaaa taatctccac gctccacttc cccagcgatt ggcgagataa tgcccaagag
    35161 actcttcaag agagttgttt ttccaatacc attagcacca ataatcgcaa ccttttgatt
    35221 gcgttcgaag gtaagattta aaggcttggt aagaggacgg tcgtaaccaa tttgcaagtt
    35281 cttggcttgg aagataaagc gccctggtgt acgagctggt ttgaaatcaa aggatggttt
    35341 tggtttctca ctttggagtt cgataatatc catcttatcc aatttctttt gacgagacat
    35401 agccatatta cgagttgcaa cacgggcttt attacgagcc acaaagtcct tgaggtctgc
    35461 aatctctttc tgctggcgtt cgtaggctgc ctctagctga gatttcttca tagcataaac
    35521 ttcttggaac tggtagtagt caccagagta acgcgtcagc tgttgatttt ccacatgata
    35581 gacaatattg ataacgtcat tgaggaatgg aatatcgtgc gaaatgagaa caaaggcatt
    35641 ctcatagttt tggagatagc gcttgagcca atcaatatgc tcagcatcca agtagttggt
    35701 cggctcgtcc aacagcaaaa tatcaggctt ttcaaggaga agttttgcca aaagcacctt
    35761 ggttctttgc ccacctgaca aagaagttac atccgtatcc atgccaaagt ccataatacc
    35821 aagagcacgc gctacttcgt caatcttagc atccaaggta tagaaatcac gactctccag
    35881 acggtcttga agttctccta cttcttccat gagagcatca acatccgcac cgtcttcagt
    35941 cattttcata tagaggtcat tgatacgagc ttcagctttg aaaagctcat caaaagccgt
    36001 acggagaaca tcacgcaccg actgtctttc agcaaggaca gagtgctgat ccaagtaacc
    36061 agccgtcaca tatttggacc actcaacctt tccttcatct ggcagcattt taccagtcac
    36121 gatactcata aaggttgatt ttccttcacc attggcaccg accaggccga tatgttctcc
    36181 cttgaggaga cggaaggaca catcttcaaa aattgcacgg tcaccaaaac cgtgactcag
    36241 atttttaact tctaaaatac tcattttaat tccttatctt gtttttatgt aatcgtttat
    36301 aaaggagcca agccagatag ccacccaaag tgttggtcca caaatcatca atctcaaaga
    36361 cgcgattgaa atcaaagaaa aagtccaaga ttaattgcgt acactcgatt cccagactca
    36421 caagaaaact aaaaagaagg accttttttg ttttccgcaa atttggaaat agataaagga
    36481 gttggaaaat cagaggaaaa aacaagaaga cattgaggat attttgtaaa aaaatccaac
    36541 ataattgtcc aatgtcactc acttcgccca gtttccagag agaattgaaa ggagtcaaaa
    36601 gaaaaaccag gcgtccaaga tgctgaatac ctggagttcc cacttccacg gtagcttgag
    36661 gagtaaagca aaaatagaca atgcataggc tataaacagc cactcccaat cttaagatta
    36721 attctctttt tttagtcatt ttacgtattt accgctgcga ctgccttctg gcggtcacgt
    36781 ttcattgtgt taaagcgcaa ttgtccacaa gctgcgtcaa tatctgtacc atgctcttga
    36841 cgaaccacac agttgccccc ttttttctta agcgtatcat agaaagccaa cacgcgctct
    36901 ttgggactac ggctatattg gtcatgctca ctaactgggt tataaggaat caagtttaca
    36961 taagacaatt tcttgatgtt cttgagcaat tcagccaatt ccaaggcttg ttctacaccg
    37021 tcattgactt cattaagcat gatatattca aaggttacac gacggtttgt tgtctcaatg
    37081 tagtattcaa tagcagcaaa gagtttttca atcggaaagg cacggttaat cttcatgata
    37141 cttgaacgaa gttcattgtt gggtgcgtga agagacacgg caagattgac ctgaacccct
    37201 tcatcagcaa agtcacgaat tttatgagcc aaacctgagg ttgaaaccgt gatgtgacga
    37261 gcaccgatag ccattccttt atcatcattg atagtacgaa agaaattcaa gacattgttg
    37321 taattatcaa agggctcacc gattcccatg acaacgatat ggttgacgcg ttcatcctga
    37381 ccacgctcag caaagtattt ctgaaccagc atgatttgcg ctacgatttc accgttattg
    37441 aggtcacgtt gcttcttaat caaaccagag gcacagaagg tacaaccgat attacagccg
    37501 acctgagtgg tcacacagac agataaacca tagtgttgac gcatgagtac agtctcaatt
    37561 aacataccgt caggcaattc aaagagatat ttgactgtac catcagcaga ctcttgcaca
    37621 ataccttgtt tcaagggatt gaccacaaac tggtcattga gcttagcaat caaatccttg
    37681 gaaaggttgg tcatttcttc aaatgactgc acacgtttac ggtagagcca ttcccagatt
    37741 tgatctgcac ggaatttctt ttctccctgc tccaataccc attcctgcat ggtttgatgt
    37801 gtcaaactat gaattgaagg tttcatttct tctccttatt ctctactcac ttctgacgaa
    37861 tgacaaaatg acgttgtccc ttgtcctctt tctgagaatg ctggtctttc tgacgacgtc
    37921 tattcttctt atctgcattc gactttcgtt tagtttgagt cggtttcttt ccttttctag
    37981 aaggtgtttc ttcttccgtc ttacgcattt tcttgtcaaa tgatgctcgc ttaggggctt
    38041 cattttctaa gacaaaatag gcacaaccat aactacaata ctctaaaagg tagtcttgta
    38101 aacgactgat tttttcaagt ttttcttctg ttcggtcatc cttgtaaaaa cctcgtaggc
    38161 gaagctgttc gttgctccag tcccccacga tataatcaaa cttggttaag acttctgaaa
    38221 aacgctgatt aaaagtcgtc acatcaaagg catccttgat attttcaacc aaggaaaaag
    38281 ctatcccttc cgtttcgacc ttatccccgt gtaaatggaa ctccggacca ggaaacttgt
    38341 tatagttgta taattcaggt gcaatttctt ttcgcataga tatccttttt ccacgattac
    38401 ttaatacttt attctaccat aatttctagc agttagcacg tttctcataa aaatgaaaaa
    38461 agtctaacga ttttgtcaga ccagaatctt ataacctaaa aaagagaaga acaattcttc
    38521 cctccaacta tcattattta gcagctgcgt acaattcatc tactttattc cagttgatta
    38581 ctgaaaagaa agctttgatg tagtcaggac gcacgttgcg gtatttcacg tagtaagcat
    38641 gttcccaaac gtccaagccc aagattggtt ttttaccttc tgagattggt gtgtcttggt
    38701 ttgctgttga agtcacttca agtttccctt ctttgttgac aaccaaccat gcccaacctg
    38761 aaccaaaacg agttgttgct gctgcagtga aggctgcttg gaattcttca aatgaaccaa
    38821 atgttgcatc gattgctgct gccagttctg ctgaaggagc tgttttctcg ggagtcatca
    38881 attcccagaa aagagcgtgg ttcaagtgtc cgccaccatt gttgataagt gcttgacgga
    38941 tatcagctgg gatagattct acatcagcaa gcaaggcttc aaggtcttca ccgatttcag
    39001 ggtgtttttc taaagctgca ttggcattgt tgacataagt ttgatggtgt ttgtcatggt
    39061 gcaaatgcat tgtttccgca tcgatgtatg gttccaaagc gtcgtatgca tatggaagtt
    39121 ctggtaagat aatagccatc tgtaatacct ctttttcttt ctatatgaaa atgataacgc
    39181 aaacattcac ttttttcaag ttttttgctt atagataagg aaatcactga catactttct
    39241 aataaaataa gcaaatcagc aagaaaccca cgacaagata atcctgacgc gggttgtagt
    39301 ttcaacaaat gtcaattgac ctgactagca atctgtaaga gtgccttttc aaaaaggaaa
    39361 cctttttcat aaagacctgt cttaatctga tagtctgtct caatcaaata ggaaatagct
    39421 tgcttcagaa agctcaaaga aagtcctctc gaatctctta atgcaaactt gatttgataa
    39481 ggatttgggt tacgtcccag ataactacct aaactacttg caatctgcga ttctgtttgg
    39541 ccagactccg ccaaaatctt cacctgagta aaagtccgaa attgtcccag catgactgca
    39601 atcagtttga tttcatcttc cccttgcaag gtcaagtctc tcaccaaatc gcgcgcctga
    39661 tccatctttt tagtcagaat aaactgagtt aaatcaaaaa tattgtcctg caaggtcttg
    39721 ggaattgcgt taacaatatc ctcttcctcg ataacagaat ccgccttata ggactgtaaa
    39781 aagagaagat ttttctggat ttcgctaaat tgaaaccccg acttgatgag gagattttca
    39841 aaagaatgat tggtaaactg cagaccttgt ttctgactcc acttttggaa atactggcgc
    39901 aattcttgtt cttttacttc tactgcatcg aagaccttgg catcacgctt aagtaatttg
    39961 actaaccgtc ttttgctatc cagctttcct tctgcaaaga ttatcaactt ggttgttgga
    40021 gaaggattgt caaggtattc ctcaaatgac ttaagctcat catctgtcaa aaagcgtttc
    40081 ttagcagtcg tgatatccat aaaataatct aatatcacga ttttctcatc cgcaaagaaa
    40141 ggaaggctga ccaactccag ttccacatcc ttgtaaacta cttctttcat atcaaagtag
    40201 gcaaagttga ggtcagcaga atcataccca atctgtttca acatttgact cttcatcact
    40261 tcaaactgac cctgatctgt ccctgtaaat aggctcaggc tcggtaaatt tgataaagtc
    40321 aacttctgac tttcttcaat ggctagcatc gtctctcctt tcttcagatt tttcgattta
    40381 atttagtcaa tatagcgcaa tttcccacgg aaatcttcta agctctcgta gcctttttcc
    40441 accatgattg ctttcagttc attggtaatg cggtcaaaag cactgacgcc ttctttgtga
    40501 agggtcgttc ccacctgcac catacttgct ccacagagga tgtgttcaaa ggcatctcga
    40561 ccagtcagaa cgccacctgt tccgataatt tggatttgag gatttaaacg ttgataaaag
    40621 gcgtgaacat tggctagagc agtcggtttg atgtattctc caccaattcc accaaaacca
    40681 ttcttaggcc gaataacgac agattcgtct tctatataga ggccgtttcc gatagagtta
    40741 acgcagttga caaacttgag cggatatttg ttgaaaatag ctgccgcttg gtcaaagtga
    40801 acaatatcaa aataaggtgg caatttaatt ccaagaggtt tggtgaagta agcaaacact
    40861 tctgccaaaa tccggtctgt tgtctcaaaa tcataggcaa tctgaggttt acctggaaca
    40921 tttggacagg aaagatttag ctcagtcaga ccacgaaaat cactctcttg gacttttttc
    40981 aaaatagtat gggtttcctc tggagacatg ccgaccagag ataagaagaa agttcggttc
    41041 gactcttttt cctgcaaatc caaaagataa tccaaataat agtctaagcc attatttggc
    41101 aagcccatag agttgatgga accaagtgga acatcttggt agcgtggctc aggattcccc
    41161 tgacggaagt ccaaggtcgc tgtcttagta acaaaggttc ctgccgctga gtttttgacc
    41221 tcttctaact cctctatcgt catacaagcc acacctgctg cattcatcaa gcaattgtca
    41281 aactcaaaac cagcaatttg tgttttcgtt gataccatga ttctaatcct ccagatttct
    41341 tttattgcta atattatacc ataattttaa ttctaggttt actagattct gtggttctat
    41401 ctatttattc ggaaaagaaa gtctagtaca gaagtcatgc tatggaaaca gctagtgtat
    41461 tggaagtttg aacaaggctt gggtaacaga cttttgaatg aagtccaagc aacaaggaga
    41521 aatctcaacc atgagtccat tccatgactc tgttgctcta gaaaaccttg caatagtcaa
    41581 gtgataacga ataaaacttt ggattgaaat atctccatga ttatctatta cgaggagaaa
    41641 tctcaacctt tatcattttt aaataaatat agcaaatata gtaaaatgaa acaagaacag
    41701 gagaaatcga tcaggacagt caaattgatt gctaacaatg ttttagaagt tgaggtgtac
    41761 tattctagtc tcaatctact atacatgtaa atctctactt tcgcaaaatg gattttcaga
    41821 acagtatccc agtatagtta aaaactataa aaaagagagg acgttcccct cttaaaatct
    41881 tattttactg cttctttcaa agcatctacc ttatccaaac gttcccatgg aagatcaata
    41941 tctgtacgtc ccatgtgacc gtaagccgat gtttgacggt aaattggacg cttgaggtcc
    42001 agcatttgga taatccctgc agggcgaagg tcaaagattt gacgagccgc tttttcaagt
    42061 tgactttcag ctactgttcc tgtaccgaaa gtatcgatac gaacagaaac aggttgcgca
    42121 acaccgatag cataggccaa ctgcacttct gccttcttag caaggcctgc tgcaacgata
    42181 ttcttggcaa tatagcgagc cgcataagag gctgaacgat ccaccttagt cgcatcttta
    42241 ccagagaagg caccaccacc atgacgagag tagccaccat aagtatctac aataatctta
    42301 cgaccagtca aacctgagtc cccttgagga ccaccgatta caaaacgacc tgtcggattg
    42361 ataaagaatt ttgtcttatc atcaagataa gaagatggaa taacttcttt gatgaccttg
    42421 tcaatcacat cttgatggat ttgttcatta gtggcctctg gatcatgctg agtagaaata
    42481 acgactgtat ctacacgtac cggacggtca ttttcatcgt actcaactgt aacttgtgat
    42541 tttgcatctg gacggagata gctaatttct ccagacttac gaagttctgc cagacgacga
    42601 accaatttat gactgagtgc aattggcaat ggcataagct cttctgtttc atctactgca
    42661 aatccaaaca tgagcccttg gtcacctgct ccaatcaagt ccagtggatc ttgatcagca
    42721 tttccacgaa cctccaaggc ttcgttaaca ccttgagcga tgtcaggaga ttgttccacc
    42781 aaagatgggt gtactcccac cgtctcagca gaaaatccat attctgtatt ggtataacca
    42841 atctctgcaa tggtatcacg aaccacacgg ttaatatcca cataggcatt tgtagaaatt
    42901 tcaccaaaaa cgtggacaga accagtatat acagctgttt cagcagcaac gtgcgcctct
    42961 ggatcctttg ctaaaatagc atccaaaatc gcatctgaaa tttggtctgc aatcttatcc
    43021 ggatgcccct cagatacaga ttcagacgtg aataatttac gttctgacat aaaaatgtcc
    43081 ccccttaaaa aatattgtta tagagttaca aagtactgat aggaaaactc ttcgtaataa
    43141 atacctacca tcttatcggg actatataac tttatcatta taccattttt aatcgcaatt
    43201 tgcagtcttt aatgtagtgt ttcggtttag atagttcgtt ctcataaagc aaaaagttgg
    43261 agctcagtac tccaacaatt tctattaaaa tgtttgacgt ttagctttac gctctgcacg
    43321 accgcgagcg cgattttcag cacgcttggt tttacggcgc ttttcatcaa ccgcccattg
    43381 aattttcttc ttataacccg gtttgacttt tttctttttc tttttaacca aaccaatcat
    43441 ttcgatatca agtttatctt gttttttctc acggttggca cgacgatcac ggtcataggt
    43501 atcttgaaat tccccgtctt tgaccatctt aggactaaac ttgattccca atttctccaa
    43561 ctcacggata tccgagtcat cacttggctg ataaagggta atagctgtac ctggtaggcc
    43621 atttcgtcca gtacgaccaa cacggtgaac aaaaaaagat aagtcttgcg gaatggcatc
    43681 attgatgaca tggctgacac cttcaatgtc aatcccacgc gctgccaaat ctgttgcgac
    43741 aatatactca aaatccagat tttgcacctg attcatgatt cgcttgcgtt cacgaggggc
    43801 aatatcgcca tggatttttg caaccttcaa gccttgagca gtcagatatg aatgcaattc
    43861 atcagcacgc gttttagtgt taacaaaaat cattgccaaa tacggctgca tcaactgagt
    43921 caactggtaa atttgagcat tcttatcatg tcccttggtc gaaatcaacc aattatcaat
    43981 ggtgtcagaa ataaccgttt tggtcttaat tttctccata acaggattgg ataagtattt
    44041 tttcaagaat ggttgcagtt tttgtgggat agtcgctgag aagaccatga attgcaagtc
    44101 ttttggaaga ctgccagcaa tcttatcaac agtttccaag aatcccatat ccaaggtcat
    44161 atctgcttca tcaacaacaa atgtcttggc tttatgaata gctaaatcac cagatttaac
    44221 caagtcgtag atacggcctg gtgttccaat aacaatatga ggctgattgc tttccaattt
    44281 ctcaatctgg cgagccttat ccgtaccacc cacataatta accacacgaa cttcgacatc
    44341 tgagtgagct gaaatctgac gcgctacttg gtaaatttga gtagccaact cacgactcgg
    44401 tgcagtaatc actgcttgta cactatcgct agcttcatct aattgctgga aaatcggtaa
    44461 caagaaagta tgagtcttac ctgaacctgt ttttgattct cctactaggt cacgacctgc
    44521 caaaacaata ggaatcaact tgtcttgcac ctctgttgga gttgtaaatt ttaactcctt
    44581 caaggcttct ctaatatagt ttttaaattg aaatttcgta aatgacataa catcctcgat
    44641 tctatctatc ttatcaatta taccatattt tattccatta cagtagtctc acttatttag
    44701 gctatttcca gtagcttctc tagtaagaaa aggttggaat tttatagttc caacctcttt
    44761 tcagttatta tttccagttt aatatagcat ttaagccata gtgatcactc acttgtggac
    44821 tcttgttacc atcaaatacg acatgtaaat tttccaccgc taactctttg gtagtaaaga
    44881 cataatcgat tcgaaggggt tcagtgttcc ctttccagcc atcaatttca ggcggaacag
    44941 tatagctacc acttttctct tgagcaactt caaatgcgtc ttgtaagcct aatagactag
    45001 ctaaaatagc ttggtaacct tcctgacctg ctgggttgtt aaaatctcca gctagtaaaa
    45061 gtggcttgtt caattttttc aagacagcct caaatcgtgc ccattcttct tggaaacctt
    45121 tatcccacca agagagatga acactggcaa ctgctagctc cttgccatcg actacagttt
    45181 cagctagggc aacacggcga gtatgatagt ctgttggatc atccacatct gaaaccaaaa
    45241 tttctctggc ttcaataggt gttttagaca agatagccac accttcgtgg tagcggtcat
    45301 agccgatatg gttataagcc caggtccagt agtaattttt cccttgctct aacaactttt
    45361 caaccaagag tctaacataa tggtcttggt gaataggctc agctgctggc aaagcttgat
    45421 aaaggtcatt aacctccacc tctgacgagg tcatctcctg attgatttct tggaaacaaa
    45481 tcaaatcata gtccttttca agaatatctt caagcaaaat ctggaatttt tcctctgctt
    45541 ctttctccat ccaactgtga gtattgagtg ttaaaaattt cattcttttc tcccaaaata
    45601 agaggttgga atacagcttc caacctcaag tatattacaa ttctacttta gcaactgctg
    45661 ttttagctgc aagagaacct gttttttcta atttaactga tttaattgca tcaccatttg
    45721 tgaagacaac tactgttgaa gtttcacgtc ctgctgcacg gatagcatcc aagtcagctg
    45781 tgacaaggag atctcctgct gcaacttttt gtccttcagc aacatgaact gtaaatggtt
    45841 taccttcaag acttactgtg tccaaaccaa tgtgaaccaa tacttcaaga cctgcttccg
    45901 tcacaatacc aaaagcatgt tttgttggga agatgcttga cacagtacct gaaactggag
    45961 atacaatgtt tccatttgca ggttctactg caaatccatc acccatcatt ttttgagcaa
    46021 atactggatc ctttacttgt tccaaaccaa caacttgacc gtctgctact gaataaactt
    46081 cctcagtaag atctttgaag tgaacagtgt tttgttgtgc ttcagtcatt tggcttggaa
    46141 gagtttcagg aatgatttca cctgaatcaa ggatatcttg gatatcagat ttcaaaatgt
    46201 cagcttttgg accgtagata gcttgaaccc cttgtccttt catcacaaga cccatagctc
    46261 cttctgcttt ccattgctct gcatttccta ctttatctgc atctttaaca gttacacgaa
    46321 gacgagtcat acatgcatca acatcaacga tgtttacacg tccaccaaga aggttgataa
    46381 tgtttacagc ttgagagcct gctgcaactt tcacttcgct gctggtttct tctgaacctt
    46441 cagcagtttc gtagtttccg ttgcgccctg gagttgcgta attgaatttt tgaatcatga
    46501 agtttgcgat aaagtacatg attacagcaa agagaacagt tacccaaacg aagttaacga
    46561 tatccatacc aataccagca ctgattgcaa taggtgtacg agtcaagaac tcgattgaac
    46621 cgaatgagtg catacgtagg tttacgacgt cagccatagc gaaggcagca ccttgaacaa
    46681 gtgagtaaac aagatacata ggtgttgcga tgaacatgaa catgtattcg attggttcag
    46741 taacccctgt caagaatgtt gcaagagctg ttgcaatcat catacctttg tatttatgtt
    46801 tcttgtcagc atcaacatta cggtagatag caacaatcac acccatcaag ataccgaatg
    46861 aaccgatcat ttgtccaact ttgaaacgag ctggatgtac tgtatctaac aagtgttgat
    46921 attgactagc atcagtacct ttaaggttta caaggtctgt tacccatgca agccatagtg
    46981 ggtcttgacc gaatacttga gtacctttag ctgtaccagt taaaatgtca taagtaccac
    47041 caagagctgt gtagttcatt gggatagtca acatgtggtg aagaccaaat ggcaagagca
    47101 aacgttccaa agtaccatac aagaatggtg caagaattgg agcagtttct tgtgagttgg
    47161 caatccagat accgaagtta ttgatacctg tttgaactac tggccagaaa gcagcaagta
    47221 gaattgcagc gattgctgaa cgaagaataa ctacaaatgg tacgaaacgt ttcccgttga
    47281 agaatgaaag tgcatcagga agtttacgga agttgtagta tttgttgtaa gcagttgccc
    47341 ctacaaaacc tgagataatc cctacgaata cccccatgtt caaggctgga gcttcaagaa
    47401 cactgataaa gtaatcagca actttgattg aaccaccaaa gaaagtagtt accatagcat
    47461 ctggattttt caacatatcg cctgatacac caaaaattgt accagtgata cggttaatca
    47521 agatgaaggc aagaccagcg gcgaaagcac caccagcacg ttctttagcc cagcttcctc
    47581 caatggctag ggcaaacaaa atgtgaaggt taccgataac cccccaaccg atttgctcaa
    47641 gaattccacc tgtgatgaca agtggtgtaa aggttgggtt aatcatcacg atagacttac
    47701 cgattgaaat catcaaacca gcagccggca taaccgcgat aactaccatc aaagccttac
    47761 cgaatttttg ccaaaattcg aaagacaaga catttttgaa tgtatctttc atcattcaaa
    47821 tctcctttat ttttatttag gcaaacgttt tacgaaaacg tttgcacctt ctatgattta
    47881 attataccac tttaattttt tttgtaaagg cttttataaa aaaattacac ttttttctaa
    47941 aaaatttct
//
LOCUS       DAVKQL010000008        42978 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVKQL010000008 DAVKQL010000000
VERSION     DAVKQL010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 42978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42978
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.008"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(125..1759)
                     /locus_tag="VW561_000372"
     CDS             complement(125..1759)
                     /locus_tag="VW561_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942705.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNTGKKVNVSARV
                     LSSYIFSSLLMLFMFINIAFRFPFEEMPSFFSTMVAILLVLAFSTSFTAFYNVFYESK
                     DLASYRPYAFKESEIIMAKGLSVLLPALAGIVPILAYFLVLYIRLAPSLWLGLPLMLL
                     SLTLLFVSVTLVMVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSTEALVGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVITVLFTSAFLPYL
                     MMISLISKIRDSQIVPDIHPPYWLPLFFVGVFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIFAVQSFLPVLTLLGLSLYLGLSILSMIYLLVVWIIASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAINLGVGALIVLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(1740..2489)
                     /locus_tag="VW561_000373"
     CDS             complement(1740..2489)
                     /locus_tag="VW561_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9942706.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLTSSDLETSLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSAPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVEN
                     LRKDHPDQSLESIYLSLAGRKEEVSDASQGH"
     gene            2668..4122
                     /locus_tag="VW561_000374"
     CDS             2668..4122
                     /locus_tag="VW561_000374"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEU9942707.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKTQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNDFHWHWYHFT
                     GTDYDAKRRKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYNW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(4212..6830)
                     /gene="alaS"
                     /locus_tag="VW561_000375"
     CDS             complement(4212..6830)
                     /gene="alaS"
                     /locus_tag="VW561_000375"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU9942708.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGQ
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNTSQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKIVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDAKEVNGRRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(6852..7337)
                     /locus_tag="VW561_000376"
     CDS             complement(6852..7337)
                     /locus_tag="VW561_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEU9942709.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSQISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(7612..7851)
                     /locus_tag="VW561_000377"
     CDS             complement(7612..7851)
                     /locus_tag="VW561_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942710.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTAILFHLNDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(8112..9182)
                     /locus_tag="VW561_000378"
     CDS             complement(8112..9182)
                     /locus_tag="VW561_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9942711.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYEAFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKLEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDPQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQDS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(9179..9952)
                     /locus_tag="VW561_000379"
     CDS             complement(9179..9952)
                     /locus_tag="VW561_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9942712.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIIAGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(9949..10755)
                     /locus_tag="VW561_000380"
     CDS             complement(9949..10755)
                     /locus_tag="VW561_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9942713.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(10736..11893)
                     /locus_tag="VW561_000381"
     CDS             complement(10736..11893)
                     /locus_tag="VW561_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9942714.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFEPEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(11952..12902)
                     /gene="murB"
                     /locus_tag="VW561_000382"
     CDS             complement(11952..12902)
                     /gene="murB"
                     /locus_tag="VW561_000382"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEU9942715.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARVVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESLSVAKMYAGGFTPCKR"
     gene            complement(13014..13766)
                     /locus_tag="VW561_000383"
     CDS             complement(13014..13766)
                     /locus_tag="VW561_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEU9942716.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLISLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            complement(13776..14507)
                     /gene="budA"
                     /locus_tag="VW561_000384"
     CDS             complement(13776..14507)
                     /gene="budA"
                     /locus_tag="VW561_000384"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEU9942717.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            14649..15920
                     /locus_tag="VW561_000385"
     CDS             14649..15920
                     /locus_tag="VW561_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU9942718.1"
                     /translation="MKKQSKYKEVVSYLKNGIESERFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(15940..16755)
                     /locus_tag="VW561_000386"
     CDS             complement(15940..16755)
                     /locus_tag="VW561_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9942719.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVDAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKVQLTSLTNMGEAVNELQTGKIDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGTYQSYLEKAASLTEVEE"
     gene            complement(16912..17565)
                     /gene="phoU"
                     /locus_tag="VW561_000387"
     CDS             complement(16912..17565)
                     /gene="phoU"
                     /locus_tag="VW561_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU9942720.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(17577..18335)
                     /gene="pstB"
                     /locus_tag="VW561_000388"
     CDS             complement(17577..18335)
                     /gene="pstB"
                     /locus_tag="VW561_000388"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9942721.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(18348..19151)
                     /gene="pstB"
                     /locus_tag="VW561_000389"
     CDS             complement(18348..19151)
                     /gene="pstB"
                     /locus_tag="VW561_000389"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9942722.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(19162..20046)
                     /gene="pstA"
                     /locus_tag="VW561_000390"
     CDS             complement(19162..20046)
                     /gene="pstA"
                     /locus_tag="VW561_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU9942723.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(20036..20953)
                     /gene="pstC"
                     /locus_tag="VW561_000391"
     CDS             complement(20036..20953)
                     /gene="pstC"
                     /locus_tag="VW561_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU9942724.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(20953..21831)
                     /gene="pstS"
                     /locus_tag="VW561_000392"
     CDS             complement(20953..21831)
                     /gene="pstS"
                     /locus_tag="VW561_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEU9942725.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(22405..23709)
                     /locus_tag="VW561_000393"
     CDS             complement(22405..23709)
                     /locus_tag="VW561_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEU9942726.1"
                     /translation="MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQTFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGET
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(23710..24483)
                     /locus_tag="VW561_000394"
     CDS             complement(23710..24483)
                     /locus_tag="VW561_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU9942727.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSSTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRSVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(24473..24751)
                     /locus_tag="VW561_000395"
     CDS             complement(24473..24751)
                     /locus_tag="VW561_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEU9942728.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(24751..25152)
                     /locus_tag="VW561_000396"
     CDS             complement(24751..25152)
                     /locus_tag="VW561_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEU9942729.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(25362..25979)
                     /locus_tag="VW561_000397"
     CDS             complement(25362..25979)
                     /locus_tag="VW561_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEU9942730.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLTLVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            complement(25969..26742)
                     /locus_tag="VW561_000398"
     CDS             complement(25969..26742)
                     /locus_tag="VW561_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEU9942731.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKENPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(26753..27490)
                     /locus_tag="VW561_000399"
     CDS             complement(26753..27490)
                     /locus_tag="VW561_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEU9942732.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIAEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKV"
     gene            complement(27495..28625)
                     /locus_tag="VW561_000400"
     CDS             complement(27495..28625)
                     /locus_tag="VW561_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEU9942733.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(28709..29098)
                     /locus_tag="VW561_000401"
     CDS             complement(28709..29098)
                     /locus_tag="VW561_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU9942734.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIHITEE"
     gene            complement(29114..29497)
                     /locus_tag="VW561_000402"
     CDS             complement(29114..29497)
                     /locus_tag="VW561_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEU9942735.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(29498..30286)
                     /locus_tag="VW561_000403"
     CDS             complement(29498..30286)
                     /locus_tag="VW561_000403"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEU9942736.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(30279..31214)
                     /locus_tag="VW561_000404"
     CDS             complement(30279..31214)
                     /locus_tag="VW561_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEU9942737.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINIADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(31344..31520)
                     /locus_tag="VW561_000405"
     CDS             complement(31344..31520)
                     /locus_tag="VW561_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEU9942738.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(31600..32109)
                     /locus_tag="VW561_000406"
     CDS             complement(31600..32109)
                     /locus_tag="VW561_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942739.1"
                     /translation="MNKEEFLKIKEAYKSARTEERKSIIGFITKKKDKEGNFLFTKSK
                     DKPYTTRNQYSGGGGNKKYTSGSRLSRPYDLSNHMWIDLSYKGNDILISLQSFDIDPN
                     SKELHVLYDRIGILFEQSKKIPIFKDCYTITKVSDAFLKMETTNWELPLSKADMEEMV
                     NYIINHYEE"
     gene            complement(32187..32894)
                     /locus_tag="VW561_000407"
     CDS             complement(32187..32894)
                     /locus_tag="VW561_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEU9942740.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FTKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            33045..34073
                     /gene="queA"
                     /locus_tag="VW561_000408"
     CDS             33045..34073
                     /gene="queA"
                     /locus_tag="VW561_000408"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEU9942741.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRIIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDAMFIY"
     gene            complement(34734..35123)
                     /locus_tag="VW561_000409"
     CDS             complement(34734..35123)
                     /locus_tag="VW561_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9942742.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTGWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNTSGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(35177..35869)
                     /locus_tag="VW561_000410"
     CDS             complement(35177..35869)
                     /locus_tag="VW561_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9942743.1"
                     /translation="MNTSFVHAVDGIQYVRDDTRDKEEGIEYDDADNGDIIVKVATKP
                     KVVIKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTRTRTYDVNPETGHVTEQVTVD
                     RKEATDTVIKVPAKSKVEEVLVPFATKYEADNDLSAGQEQEITLGKNGKTVTTITYDV
                     DGKSGQVTESTLSQKEDSQTRVVKKGTKPQVLVQEIPIETEYLDGSTLDKSQEVEEVG
                     EIGKLLLLQSIL"
     gene            complement(36585..37136)
                     /locus_tag="VW561_000411"
     CDS             complement(36585..37136)
                     /locus_tag="VW561_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9942744.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDVEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGHGMMTEALKAVLDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(37534..38358)
                     /gene="nadE"
                     /locus_tag="VW561_000412"
     CDS             complement(37534..38358)
                     /gene="nadE"
                     /locus_tag="VW561_000412"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEU9942745.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(38355..39815)
                     /locus_tag="VW561_000413"
     CDS             complement(38355..39815)
                     /locus_tag="VW561_000413"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEU9942746.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(39931..40419)
                     /locus_tag="VW561_000414"
     CDS             complement(39931..40419)
                     /locus_tag="VW561_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942747.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYIIYLPWIFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(40409..40618)
                     /locus_tag="VW561_000415"
     CDS             complement(40409..40618)
                     /locus_tag="VW561_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9942748.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            complement(41003..41731)
                     /locus_tag="VW561_000416"
     CDS             complement(41003..41731)
                     /locus_tag="VW561_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9942749.1"
                     /translation="MKLNKLNFLKENIRNLYSSGVIYLSLLISFIPPILLTFFILKTQ
                     ETSLGIKHISNFYAMLGMLMAVIHANRVISRDFSHNTVSLFYNQQKNRMIYVLSNFLY
                     AISVSIIYALNGIVLLVIVSKLGIPGDLGLDFIVAIVVNTILLVLFYFLLSYIFYLYK
                     LKSGLVFGILVALLLFIPNILNTMMMNTSNDLFIKAIELLPFYSLPVFVASNTMSISQ
                     YLVVITTIILLYFFTLKKSKKYSF"
     gene            complement(41747..42538)
                     /locus_tag="VW561_000417"
     CDS             complement(41747..42538)
                     /locus_tag="VW561_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9942750.1"
                     /translation="MNFALNKGEIVGLVGRNGVGKSTLMKILVQNNQPTSGNIISSDN
                     IGYLIEEPKLFLSKTGLENLKYLSNLYGVDYNQERFRSLIQELDLTQSINKKVKTYSL
                     GTKQKLALLLTLVTKPDILILDEPTNGLDIESSQIVLAVLKKLALNENVGILISSHKL
                     EDIEEICERVLFLESGLLTFQKVGKDSHNFLFEIAFSSATDRDIFITKQEFGDIVQEE
                     GLRITMSGNIQSSELFKFFNENSIKVVDFETKKETLKDIYLNRSK"
BASE COUNT        12915 a         9643 c         7926 g        12494 t
ORIGIN      
        1 atttcttatt caaaacattc ctccctatat ttttgattac caatcttaat catttacaac
       61 tacattctaa caaactataa aagcgtttgt cgaattgaat ttatcaagca agcaaccaac
      121 tatctcatct tttttctatt tctgccaata tgcgtgacag gtaataatga tagccaaaaa
      181 tagcaagagc aagcaagacg ataagagctc ctactcccaa gttgatggca aggatagggg
      241 agagagactg aaccaagaat atgctcccaa ttacaagggc catcaggatt gcactataaa
      301 taaacaataa aactatggcg actatgccat ttgaacgatt caccaggtcc gtaatgctac
      361 tccaattggt tgacagattt ttaacgtcct taaagtaatg gtggcaagaa aggatgacac
      421 tggcaatgat ccagactaca agaaggtaaa tcatcgaaag gatggacaag cctagatata
      481 gagaaagacc gagcaaagtc agaactggta aaaaggactg aacagcaaag ataatccaaa
      541 atttcacttt cacataacga gcaaagtcaa agggtaaact cttcagaaaa tcaacatttt
      601 ccctctccaa ggacaaggca attgaatgca ggctggtgat attgttattg acaactgcta
      661 taaacactcc tacaaaaaac aagggtaacc agtatggagg atgaatgtct ggaactatct
      721 gagaatctcg gattttggaa atcagactga tcatcatgag ataaggaagg aaagcacttg
      781 taaaaagcac tgtaatcaca ccagtcccct gtcccaagag ggtgaggtgg tagcgtaaaa
      841 ccatacggaa aaagcccttt ttagtagttg aaatcctctc cttgctgcga cgttcttttt
      901 tgaccttctc ctcactgtta agcaggatca cgtcataaaa acgaggaagg accttctttt
      961 tggtcagata aagcaggaag agagttagtc ctatccaagc gagcagaccc actaaggctt
     1021 ctgtcgaaaa aggctccact gctattttgt aaaagatatg aagaggataa aggagaaatg
     1081 gaatgtctct aactttgtca acaatacttc caaaagtcga ctgaagaaag aagataaata
     1141 ttaaaggtat gagaactcct atcccaatca tcacattcga aaaaatagac tgatactttc
     1201 tgaagaccct agtctgagcc aagaaatgta ctgccactac catcactaaa gtaacagaga
     1261 caaataataa ggtcaaggac agtagcatca aaggcaaacc caaccacaga gaaggtgcta
     1321 gcctaatgta gaggaccaga aaataagcta ggattggtac aattccagct agagctggca
     1381 agaggacaga cagacctttg gccattataa tctctgattc tttaaaggca tagggcctat
     1441 aggaggccag gtccttactc tcataaaaga cattgtaaaa tgcagtgaaa gaagttgaaa
     1501 aggccagcac cagtaaaata gcaaccatcg tactaaaaaa acttggcatt tcctcaaaag
     1561 gaaaacgaaa ggctatattt ataaacatga aaagcatcaa gagactggaa aaaatgtaag
     1621 aacttaggac tctagcggaa acatttactt tttttccagt atttttagct tgcttctttc
     1681 gtaggttagc cagattagct tcttgagatg aatagaggat attgatatca actaattttt
     1741 taatgacctt gagacgcatc tgaaacctcc tcttttctac cagcaaggct aaggtagata
     1801 ctttccaaag actggtctgg gtggtctttc ctcaagttct ctacactacc acaatagatc
     1861 aaatgcccct ttttcaaaat agcaatccga tcacagactt gctcggctac ctctaggaca
     1921 tgagttgaaa acaagaccgt cttgcctttt tgtgcatgtt ccttcatcat ttgcttcaaa
     1981 tcaaaggcgg cctggggatc caaaccagtc aagggttcgt ccaaaaccca aatatcagga
     2041 gcagacaaga gtgccccaat gacaaagact ttctgacgca ttccgtgaga aagagtttca
     2101 ataacctgat agcggttttc agcaaaatca aaaacgttca atagcctagc tagactagtc
     2161 tccaagtcag agctagtcag atcataggat gaagcgatca attcccaaaa ttcattggcc
     2221 gttaagcgta aaaataagtc aggcgagtct gctacgtagc caatctttcg tttgatcgcc
     2281 aagcgatttt ccgataactc ctgaccgtct accaaaatac gaccactgct gggtgaaatg
     2341 atactgacta gggattttat ggtggtcgat tttccagccc cattatgacc aatcaagccc
     2401 ataatctctc cattttcaat ctgcaaattg agattactca aggcctcttt atcaccatac
     2461 aacttactaa cattttgaaa ttcaatcatg ggacaactcc tatctttatc tgtattatac
     2521 atcgctatac tagcactttg atacaaaaaa atcaatactt tattttaagt agttaaaata
     2581 gtttccttct atcttacgca aacgtttgcg ctaatagatg ctttatgata gaataaagaa
     2641 caagattgac aagtaagagg aaacatcatg caaaatcaaa cactcatgca atactttgaa
     2701 tggtatctgc cccacgacgg tcaacactgg acgcgtctgg ctgaaaatgc tccacaccta
     2761 gctcatctgg ggatcagtca cgtctggatg ccaccagcct tcaaggcaac caacgaaaaa
     2821 gatgtcggct atggggtcta tgacttattt gacttaggag agttcaacca aaaagggact
     2881 gtccgcacca agtatggttt caaagaagac tatcttcaag ccattcaagc ccttaaaaca
     2941 cagggaattc aacctatggc cgatgtagtt ctcaaccaca aggctgctgc tgatcacagg
     3001 gaagcctttc aggttattga agtggatcct gtagaccgta cagttgaact tggagaaccc
     3061 ttcaccatca atggctggac tagttttacc ttcgatggtc gccaagatac ctacaatgac
     3121 ttccactggc actggtacca cttcacaggt acagactatg atgccaaacg ccgtaagtct
     3181 gggatttatc tgatccaagg ggacaacaag ggctgggcta acgaggaatt ggtcgataac
     3241 gaaaacggaa actacgacta cctcatgtat gccgacctag actttaaaca tcctgaagtc
     3301 atccaaaaca tctataactg ggctgattgg ttcatggaaa cgactggtgt agctggtttc
     3361 cgtttggatg ccgttaagca tattgactct ttctttatgc gcaacttcat ccgcgatatg
     3421 aaggaaaaat atggtgacga tttctatgtt tttggtgaat tttggaaccc agacaaggaa
     3481 gccaatctgg actatctcga aaaaacggaa gaacactttg accttgtcga tgttcgtctc
     3541 caccagaatc tctttgaagc cagtcaagct ggcgcaaact atgaccttcg tggcattttc
     3601 acagatagcc tggttgaact caagcctgac aaggctgtga cttttgtcga caaccacgat
     3661 acccaacgag gacaagccct tgagtctacc gttgaagaat ggttcaagcc agcagcctat
     3721 gccctcattt tgttacgcca agacggcctt ccatgtgtct tttacggaga ctactatggg
     3781 atttcaggcc agtatgctca agaagatttc aaagaaatcc ttgaccgcct cctagccatc
     3841 cgaaaagact tggcctatgg agaacaaaat gactactttg accatgctaa ctgtatcggt
     3901 tgggtacgtt caggtgctga aaatcaatcc ccaatcgcag tccttatctc aaatgaccaa
     3961 gaaaacagca agtcaatgtt tgtcggtcaa gaatggacta atcaaacctt tgtagattta
     4021 cttggtaacc accaaggtca agttacaatt gatgaggaag gttatggaca attccctgtc
     4081 tcagctagat ccgtaagtgt ctgggcagtc aataccatct aatagctcat aataaccaag
     4141 ctaggtccaa gcggatttgg cttttttgta ttcacaaaaa gacctaccca aatggataga
     4201 tctttacttg attacaattt acctgctact gcatccaaca attcttggat cttagcttgg
     4261 ttgcttcctc ctgccatggc catgtctggt ttaccaccac cacgtccatc gatgattggt
     4321 gctaattctt tgacaaggtt tcctgcatga aggtcttttg tcttgcttgc tacaaggaca
     4381 ttgactttgt caccgatagc ggcaactagg acaagaagat cagagtagtc tttttgtttc
     4441 cagttatctg caaaagtacg aagggcaccg gcatcggata cagacacttg actagcaatg
     4501 taacgacgac cgttgacttc cttagcatct ttgaagatat cgcctgcggc tgcagctgcg
     4561 gctttttctt tcaactcagc attttctttt tgaagttgac gaagttgttc ttgaagtcct
     4621 tctaccttgt gaggtacttc cttgacttga ggtgctttca aggttgctgc gacagcttta
     4681 agagcatcct cttgttcacg ataggcttca aaggcttcct taccagtcac tgccaagata
     4741 cggcgagttc ctgaaccgat tccttcttct ttgacaattt tgaagagacc aatctcagaa
     4801 gtgttgccaa catgagtacc accacaaagt tcaatagagt agtcaccgat agtcacgaca
     4861 cgaacttcct tgccgtattt ctcaccaaag agggccatag ctcccatttc tttagcagtg
     4921 tcaatatccg tttcaactgt cttcacttca agtgcttccc aaattttctc gttaacttgc
     4981 tgttcaatcg cacgaagttc ctcagcagtt actgcttgga agtgggtaaa gtcaaagcga
     5041 aggaattcaa cttcgttaag agatcctgcc tgtgttgcgt ggtttccaag gatattgtga
     5101 agggcagcgt gaagcaaatg agtcgcagtg tggtttttca tgacacggtg acggcgattg
     5161 ctatcaattg ccaaggtata ttcttggttc aaggcaagcg gtgcaaggac ttcaactgta
     5221 tgaagggctt gaccatttgg ggctttctga acattggtca cagtagccac aatcttacct
     5281 gactcatcca agatttgtcc gtagtcagct acctgtccac ccatttcagc ataaaatgac
     5341 gtttccgcaa agataagaga ggcagttcct tctgaaacag ctcctacttc tgcattgtca
     5401 gcaacgatag ctaccaattt agaagacaat tggctagtat tgtagttgaa gacactttct
     5461 acagtgatgt tttgaagagt ttcattttgc atacccattg agccaccctt gacagctgac
     5521 gcacgcgcgc gttcttgctg ttctttcatg gctgcttcaa aaccttcacg gtctacagtc
     5581 ataccagctt cttcagcgat ttcttcagtc aattcaactg ggaacccata agtatcatag
     5641 agtttaaaga catcttgacc agcaatgaca gattgacctt tttctttcaa gtctgccaca
     5701 atgccttggg caaagtgttg acctgagtga agggtacggg caaatgattc ttcttcgctc
     5761 ttaacgattt tctcaataaa gtcacgtttc tcaagcactt ctgggtagta gctttccatg
     5821 atttttccaa cagttggaac caatttgtaa aggaaaggct cgttgatacc caatttttga
     5881 ccatgcatag aagcacgacg gagaagacga cgaagaacat aaccacgacc ttcatttcct
     5941 ggaagggcac catcaccgat agcaaatgaa agagaacgaa tgtggtctgc gataaccttg
     6001 aagctcatgt tgtcgccatc ttggtcataa accttaccag acaatttctc gacttcacgg
     6061 ataatcggca tgaagaggtc cgtttcaaag ttggtcttag ccccttggat aacggccacc
     6121 aaacgctcca aaccagcgcc cgtatcaatg ttcttatgtg gcaattcctt gtattcgcta
     6181 cgaggaacag cagggtctgc gttaaattgt gacaaaacga tgttccagat ttcaatataa
     6241 cggtcgtttt caatatcttc tgcaagcagg cgaagaccga tattttctgg gtcaaaggct
     6301 tccccacggt caaagaagat ttctgtatct ggtccagaag gtcccgcacc gatttcccag
     6361 aagttgtcct caattggaat caagtgactt ggatccactc ccacttcaat ccagcggttg
     6421 taagaatctt tatcgtctgg atagtaggtc atgtaaagtt tttcagcagg gaaatcaaac
     6481 cattcagggc ttgtcaaaag ctcataagcc caagtgatag cttcgtcacg gaagtaatcc
     6541 ccgatagaga agttccccag catttcaaac atggtatggt gacgcgcagt cttccctacg
     6601 ttttcgatgt cgttggtacg gatagccttt tgggcattgg taatacgtgg attttcaggg
     6661 ataatggtcc cgtcaaagta tttcttaagg gttgctaccc cagagttgat ccacaaaaga
     6721 gttgggtcat ttacaggaac caaacttact gatggttcta ctgagtgacc tttggtcgcc
     6781 cagaaatcaa gccacatttg gcgtacttgt gcactagata gttgtttcat attgtctcct
     6841 tattcacttg tttaatgtga ttggctttcc agcatttcca catagtcaat cgcgacacag
     6901 agggaaatga ctaggtctgc ataagcgtct tcaagaaccg ttacggtata ggtagaggtc
     6961 agatggaaga gttccttctt aatttccgca atcaactgat cgcgatcatc cagcaatttg
     7021 aaattcaaat cccagatatt gccctcgata cgaagaccta gattatcaaa ctcatactta
     7081 tctcgccaga aggtcaactt cttacgaatg acaaaactcg agccatcccg aagctgaatc
     7141 tcaaaacgag gaagcaaggt caagatttct ttactgatct gactgacttg ttcaccagcc
     7201 gcatcataga tggtaaaagt tttgggaatc ttaaaaaatg atccctccac ctgataggca
     7261 atttctcccc tgtcatcctt gatagcgaag cgttcgcctc caagacgaaa cttttgtttg
     7321 acaagaaatg ttttcatcaa cacctccaaa aatcaaaaga caagctcata tcacgaaggg
     7381 cgaaaaaccg cggtaccacc ttcattcaat gaacttgtca ttctcttgtt cttatgcaat
     7441 tgtatgattg agtagcatga cttcctagct tagatggctc gcagcaccgc catttctctg
     7501 gactaagaca agtgaaaatc aattctcaac tttcttatta taacgttttt ttaagcttgc
     7561 gtcaactgga aatgatctcc gttgaattag accaattccc tacatctctg attacttttt
     7621 caggatatat tttttcttac tgccattttt ctttttatcc caaattttca tattactaaa
     7681 cacagctact agaatatttc caaatataaa ggtgcctatc acccaatata tggactcagt
     7741 tgttaggtat tgtcgatcca agccatcatt taaatggaat agtatagcag tttggttaac
     7801 aatcataaag gttggccaga aacttttttt gaaaaaagta gacattttca ttatttgttg
     7861 ccgctttcta taaggttaat actcaataaa aatcaaaaag caaactagga agctagcttc
     7921 aagctgtact tgagtacggc aaggcaacgc tgacgtggtt tgaagagtat aggcttagta
     7981 tactactagg caagcaaata agcaaataaa caaataaaca actagaatag aaaaagatag
     8041 ggctctaaaa actgacttct attccttaaa aacgaaccag cttgactgat tcgtcttctt
     8101 acgtttatct cctacttccg atacatttta aactgtagga agaggtcgct atatttccct
     8161 gtccatttat ggtcaaattt ctcataaact tctaggtgtt tcatggtttc aacatcggga
     8221 tagaaggcct tatcttcctt tgtttcctct gggagcaatt ccttcgctgg taggtttggt
     8281 gttgaatagc cgacatactc cgcattttgg agagcatttt caggtttcaa cataaagttg
     8341 ataaaggcat aggctgagtc ttgattttta actgttttgg gaatgaccat attgtcaaac
     8401 caaagattgc tggcctctgt cggtaccaca taacgtagat tttcattttt ttctaacatt
     8461 tggctggctt caccagagaa ggtcacgccg attgcaacat tattctgaat catgtagccc
     8521 ttcatctcgt ccgcaacgat agccttgata tttggagtca gtttgtagag cttatccact
     8581 gtctcttcca actgctgcgg atccttggag ttgaggctgt agccgaggga attgagtcct
     8641 agtcccagca cctcacgcgc cccatcaaaa agcatgatag aattcttata ctccagcttc
     8701 caaaggtcat cccaatgctc aggcgcttca tctaccatgg tttcgttgta gacaattcct
     8761 aaggttcccc agaagtaagg gatggagaat ttattacctg ggtcaaagga ctggttgaga
     8821 aactctggtc cgatattttc gattccttca atttttgaat aatcaagcgg aaccaagagg
     8881 tcttcgtcct tcatcttgtt aatcatgtat tcacttggaa tggcaatatc gtaggtcgtt
     8941 ccaccctgct ttatcttagt gtacatggct tcgttggagt caaaagcctc gtactgaact
     9001 tgaattcctg tttcttctgt aaactgagtc aagagttcag gatcgatata gtctccccag
     9061 ttatagataa ccaatttttg actatctcga ctattgattt tactatctaa atgagtcgca
     9121 attccccaca agacaaggat aatcgctgca attcctgcta aaaatgaata gatttttttc
     9181 atgcttgctc ctccttctca cgagagataa agtaataacc tacaactagg ataatactaa
     9241 agagaaagac tagagcagaa agggcattga tttctagcga aatccccttg cgagcacgag
     9301 agtaaatctc gactgatagg gttgaaaagc catttcctgt tacaaagaag gtcacggcaa
     9361 agtcatctaa cgaataggtg aaggccatga aataaccagc aatgatagac ggagtcaggt
     9421 aaggaagcat gatttcctta aacatctgaa attgactagc tcccaagtca taggccgcat
     9481 gaatcatgtc gccattcatt tccttgagtc gaggcaagac catcaagacc acgataggaa
     9541 tggagaaggc cacatgacta gatagaacgg tcaaaaagcc aagtgaaaac ttgagttggg
     9601 taaagagaat caagaagcta gcaccaatca taacgtcagg cgcaaccatg aggatattat
     9661 tgagtgatag aaaggcttct tggtatttct tacgagactg gtagatgtaa atggcaccaa
     9721 aagtcccgat aatggtcgct atcaaggctg ataggaaggc caagaaaaat gtctgagcca
     9781 aaatcagcat gagtctccca tctccaaaca tggtttcaaa gtgagtccag ctaaaacctg
     9841 taaagctatt catatcatca ccagcattaa aggcatagcc aatcaagtaa aagataggca
     9901 ggtagaggac cagaaagacc agtcccagat aaaggttggc aaattttttc atcgttctct
     9961 cctttcctta gtcacccaca tggtgatgaa catggtcagg atgagaatca caccgatagt
    10021 tgaacccata ccatagttgt cattggttag aaaattctgc tcaatagccg tccccaaggt
    10081 gataacgcgg ttcccaccaa tcaaacgggt cagcatgaag agactcaaac ttgggataaa
    10141 gaccgactga accccacttc gcacaccgtt catagataga gggaagatga catgacggaa
    10201 ggtctcccac ttagttgcac caaggtcgta actagcattg atgagattat tatccatatc
    10261 gtctaagaca ttgaaaatcg gcaaaatcat aaaggggagc tcgatgtagc ttgcgacaaa
    10321 gataaaggaa aaatcggtaa aaagcaactg ttgtgaacca attccgataa attccaagaa
    10381 ttggttaata gagccatttt gaccaaaaat cccgataaaa gcataggctt taaggagcaa
    10441 attgatccag gtaggaagga taatcagcat gagccagagt tgacggtgct tgagacgggt
    10501 caaaaagagg gctgttggat agctgataag caaggtcaca aaggtcacaa tccctgcata
    10561 aagcactgag ttgaaactca ttttgaggta ggtcaagttt tgtgacgcaa agtaagattt
    10621 ataattttct aaactaaact ggccttcaat gttgaaaaag gattgtccga aaatcaagac
    10681 caagggtgcc aatacaaaga gggcaatcca aagcatgtag ggcactacaa agagtttaga
    10741 gcttgttttc ttcatctctt tcctcctcga tcgcattgat cagacctgct tcttgctctt
    10801 cgatttccac gtattcttcg atacgagcat cgaactcttc ttcggtttca ttgagacgca
    10861 tgatgtggat gtcttctggt tcaaagtcca gaccgatttc ctcacccacg atggccttac
    10921 gggttgagtg gatcatccat tcatttccaa gttcgtcata ggcgataatt tcataatgca
    10981 ccccacggaa gagctgggta tcgaccttaa cttggagctt gccttcttca ggaagggtaa
    11041 tgcgcaagtc ctctggacga ataacgacct caacaggttc atttggcttc atcccaccat
    11101 caaccgcttc aaagcgtttg ccgttaaatt cgaccaagta gtcctcaatc atggtacctg
    11161 gcaagatgtt tgactccccg ataaaggtgg caacaaagtg gttgattggc tcatcgtaga
    11221 tgtccacagg ggttccagac tggacaatct cgccatcatt cataacgaaa atccagtcac
    11281 tcatggcaag agcttcttcc tgatcgtgag tgacaaagac aaaggtaatg cccaatcgtt
    11341 gttgtaattc acgcaattcg tactgcatgt ctgttctcaa tttcaagtcc agcgctgata
    11401 aaggctcgtc caacaagacc acacggggtt ggttgatgat agcacgggcg atggccacac
    11461 gctgacgttg tcctccagaa agtttgcgga tggaacgttt ttcataacct tccaactgaa
    11521 ccatcttgag aacttccgct acacgctgct caatttcttt cttatcaatt ttacgcaagc
    11581 gaagtggaaa ggcaacattt tcaaacacat tcatatgtgg gaacaaggca taggattgga
    11641 agacggtatg tacgtcgcgc ttattggttg gaatatcatt gatacgaaca ccgtctagca
    11701 tgatatctcc tgtcgtcgca tccagtaaac ctgcaataat gtttaggata gttgatttcc
    11761 ccgaaccaga tgcacctaga agggtgtaga atttcccttc ttccaactca aagttgatgt
    11821 ctttgagaac cttggtgttg ctgtcttcaa aaactttaga gacgtttttg aattcgataa
    11881 ttggtttttt caattggcat aaattccttc tttttcatag attaaccgat cggggctctg
    11941 tcaggtcccc actacctctt gcagggagta aaaccacctg catacatctt cgctaccgat
    12001 aggctttcac ccaagatccg gacttctctt tcaagcgtaa tacctgagtg ttccttgact
    12061 ttttcgataa ccgattggat caagtcctcg tagtctttgg ccgttccatc tgcgacattg
    12121 atcataaatc ctgcatgctt ttctgacact tctacgccac cgatacgata gcctttcaag
    12181 ccagcttctg aaattaactg acctgcaaaa tgcccgactg gacgcttaaa gaccgagcca
    12241 caagatgggt attccaaagg ttgcttgagt tcacgtaggt gcgtcaagcg gtccatttcc
    12301 tgcttgataa cctgatgggt tcctggagct agggcaaatt taactgacaa gacaactgca
    12361 ccagactcct gaatagctga atggcggtaa ccaaaagcca agtctttagc agacagggtt
    12421 tcgatttctc catccttggt caagacctta caagactgca agatgtgagc aatctcgcca
    12481 ccataggcac ccgcattcat aaagacagca ccgccaacgc ttcctggaat accacaagca
    12541 aactcaaagc cagttaaact atgacggagg gcaatgcgag ttgtttcaat caagttagcc
    12601 ccagcttctg cttcaatggt atagccatca acagaaacgt tattgagctt gtcacacaag
    12661 atgacaaatc cacgaatccc accatcacga acgatgatat tgcttgcatt gccaagaacc
    12721 atccaaggga tattttcttg gttggcaaat ttcacaacgc gagccaactc aaaacgattt
    12781 cgtggaaaga ccaaataatc agcctctcca cctacttttg tataactata gctatgcaag
    12841 ggttccttaa aacggatatc aattccttct aagatttcaa gcattttttc tcttacagac
    12901 atgtcactct tccttttaca aaattcattc cattatacca tttttagaga catttgacga
    12961 ccataaaaat accttgtttg gattttgcat aagaaaaaga ggttcccccc tttttatgat
    13021 tttttacaaa agatttcctt ggttccatag gcgaccagaa cgagctccag tgctagaatc
    13081 acttcaacca agactggatt tgtcaaccag cctacttgga aaagagatgg tgccagatca
    13141 aagaaggcat gcaagccata ggctgctagg agataaatcc atttcttctg gcgaacagct
    13201 tggtaaaccc aaactgtcaa aagtaattgg aaaccaagcg ccaagattcg ctcaaaacca
    13261 agcaaataaa tctgccagac cgaaagtgac tgaatggttt ttaacatatt ttcagacagt
    13321 aattgcataa cctgtggatt ctgagtttga actgccgaaa gaacgatgta aagattgagt
    13381 aaactaataa ggcctagaaa aatcaactcc aagccaccat gccctaggcc ataggccaag
    13441 gcatctgcct tttccaaact tctctttttc tccaaccatt tgaagaaaac aagacgagcc
    13501 gtttcctcaa aaaatgctgc catggctagg ccatagataa tatagataag cggatgatct
    13561 tgcaagaggg caatactacc gtctttttga ggatgcaaaa tcaagatatg caccagtttt
    13621 tccaaaatct gcgaagagac aaagaaagcg actgcaccca agcccaagac agctagatta
    13681 atctggtatt tctttttggc aaaccaaatg cttcctatca gaaaagctag caacagcaac
    13741 atggtaataa tgatatgaat ggtcattttc ttctcctatt ctgccttttc aatatctttt
    13801 ttcatctcgt caacattgaa cttagcaaac aagtattgac ggtcttggac tgggaaacgt
    13861 tggtccaact ggtcaactgc tcccacctcg ataatgcctt ccttgatgac aaaatccatg
    13921 acatgtccac cgaaggtcaa atcatctgat atgaagtgca gatggtagcc tgccacactg
    13981 accccatgga aaatctcagg cgtccagaaa ccaacaatag tccccgcaac attgtcacga
    14041 ctatattccg gttgatgggt tgcgacatca gcaaacttgg tatcgggtgt tgacttagga
    14101 atcatacgca catgcatatg tgaaaattcc ccccgaatct tgatagagcg gaaaagattt
    14161 tccccatcat aataagactc aattcgttct ttcaattcct tgtctgtcat ctcaaagcgc
    14221 tggcggaaaa tgacctctgc ctgatgcggt accactgcag cgtaaggaat aagggcatct
    14281 ggtgacactt ccacaatttc tggctggtct cctgaccctt tggcctgata agccttgcca
    14341 tccaagacaa tcaattcccc atcaatggaa tccaaggttc ccaaaccaag gtcaccatgt
    14401 tctagcaatt ctcctactgt catagtacca ccataaaggc cagccatcaa ggctccaaga
    14461 gtattgtatt gaaataattt cactggttcc tgcacgttct tatccattca ctttcttgtc
    14521 tgttattcta taaatcttac tcctaagtat accacatttg cccctagatg tgaacgagag
    14581 aaacgctcta gacattgcca agaaggaaaa aaaagggtac aatgtaacaa aatcaaggga
    14641 ggtctggaat gaagaaacaa agcaagtaca aagaggtcgt ttcctatctg aaaaatggta
    14701 tcgagtctga acgatttccg acgggtagtc gcctgccttc tatccgtcaa ctgagccttg
    14761 actttcactg cagcaaggac accattcaac gagccctgct ggaattacgg cacgaacaat
    14821 acctctatgc caagcctcag agtggctact atgtattaga acaagggcaa catcaagacc
    14881 tagaaatcga ggttaccgac gaacatgcca gtgcctatga cgatttccga ctctgtgtca
    14941 atgaaacctt gattggacga gaaaactacc tcttcaacta ctatgacaat caagaaggat
    15001 tagaagacct aagacagtcc attcacaaac tcctatttga gcaagctctc tactgcaagg
    15061 ctaaccaact agtactgact tctggaaccc aacaagcctt gtttatcctc tctcaaatat
    15121 cctttcctag acaagccaag gaaatcttgg tggaacagcc aacctaccat cggatgaatc
    15181 gcctcttgat tgcacagggg ctggactatc aaacgattga acgaggcatt gatgggattg
    15241 acttggagga gctggaaggc cacttcaaaa caggaaaaat taaatttttc tacaccattc
    15301 ctagatttca ctatcctctg ggacattcct attctgagca agacaaacga tctattctta
    15361 acttagctgc caagtatgat gtctatatcg tagaggacga ttatctgggt gatttggact
    15421 ccaagaaggg ccaaaccttc cactatcttg atacagagga gcatgtcatt tatatcaagt
    15481 ccttctcgac cagccttttt cctgcccttc gtattacagc actcattctt ccaaatgcta
    15541 tcaaagaagc atttgtggcc tacaaaaata tcctagacta cgacagcaac ctcattatgc
    15601 aaaaggccct gtcactctat attgacagtc aattgtttga aaaaaatcgt ttggctcgct
    15661 tgaccaatca tgaatcttac caaaaacaaa tcgaggaaag gataactaaa acaccttgtc
    15721 cccttcctca ttatccccta cacgatggtt tattgctaga cctgagacag tatcctaaaa
    15781 tcgccagtct caaacacagt caactgggct tggacttctt tgaagaggcc tatttaagca
    15841 cctgtcctta tcaatttgcc aaggtgtccc tagacaatct ggaaaatgtt ttaaactatt
    15901 taaaagcaga attggaatga catccaactc tgctttttct tattcttcaa cttcagttag
    15961 acttgctgct ttttcaagat agctttggta agttccttca tccttaagtt tttggatgac
    16021 cttgtctacc acttctttca aatcatcact attttttcta agagcaacgg cattggcgtc
    16081 gccgtccttc atcttcaagc tgacagttgc gacagctaag ccagcgtttt tagcagcata
    16141 actaagtgca acaggctcat ccatatgaac agcatctatt tttccagtct gcaattcatt
    16201 gactgcttca cccatattag ttagggaagt taattgaact tttggcaatt gttccttgac
    16261 cattgattct ggaacagtcc ctttttgggc tgcaatatta gcactttcta ggctagttaa
    16321 atccttgtat ttttccacat cagccttacg aaccaagaaa ctaatcttgt tttcatagta
    16381 tgggattgaa aaatcaaaga cttcttttct ctcgtcagta gcactaattc ctgcaactgc
    16441 taggtcagcc ttaccagttt gaagactggt caaaacattg tcaaaactca tgcttgagat
    16501 ttccaactta accccaagtt catcagcgat agcctgagcc atgtcgatgt ctgcaccgac
    16561 tacctggttc tttccatcaa ccaatgattg aaattcaaag ggtgcatagt caggactggt
    16621 tgccacaact aatttccctt tttgtttgat agcatccaca gcagactgag aaccattaga
    16681 acctgactga caagctacta agaagaaact tgcaagaaaa ttacataaaa caaatatcca
    16741 tttttttgat ttcataaata aacaacctct ctgttaattt tgtataatta taacgatatc
    16801 caagttactt gtcaagtgtt ttttaaattt ttatctcaaa aatatttttt cgttcaaaaa
    16861 aaggagccat cagttgattt caagctcctt tttatacaga attaaactat tttatagttc
    16921 gacaatctta cctgtttcaa agtagacaac ccattcacag atatttttag catagtcacc
    16981 gatacgctcc aagtaggaaa taacttggaa ataatcacga cccgtaacaa tggcttctgg
    17041 atttttctta atctcttcag tcgcaaggtc acggatagtt tcaaaatagt ggttaatttg
    17101 ctcatccatg gaggccaccc ggtatgcgtc gtcaacagaa ccattaagat aaagatcaag
    17161 tgctgcttca acaacgcttt taacttcacg tcccattttt ttaatttctt cctctacagc
    17221 tggaatgcgc tcttccccct tcatacggat ggttgcctgg gcaatggcta cagcgtgatc
    17281 ccccatacgc tccacatctg atacagcctt aaggacagtc aagactgtac gcaaatcttg
    17341 agagactggt tgttggagtg cgatcatttc aaatgatttc ttttccagtt tcacttcgta
    17401 ttcatttact tctgcatcat cttcgatgac ctcttttgcc aggtcacggt catgcgtgac
    17461 aaaagcacgt accgtacgat tgatttgtga gagcacttct tgtcccatag cgtagaactg
    17521 gttatgtaat ttctctaaat cttcttcaaa ttgagatcgt aacatctttc atctccttat
    17581 ccaaattttc ctgtaatata gtcttccgtt tccttgtgtt ggggatcaag gaacatctgc
    17641 ttggtatcat taaattcaat caaatctcca tctaggaaaa atcctgtctt atcagagata
    17701 cgtgaagctt gctgcatgga acgggttacc agaagcatgg tgtacttgtc ttttagacca
    17761 tacaaggttt cctcaatttt accagctgaa atcggatcca aagccgaagt tggctcatcc
    17821 aagaggatga ttttaggact agttgccaag acacgggcca cgcagacacg ctgctgttga
    17881 ccacctgaca atccaatagc tgaatcatat agacgatcct tgacctcatc ccagatagag
    17941 gcaccttgca aggctttttc tacggcttca tccagaacct gcttatcctt aattccattg
    18001 atacgaagcc cgtagacaac attctcatag atagtcatag ggaaaggatt aggttgttgg
    18061 aaaaccattc cgatttcctt acgtaattca accgtatctg tacgcggact gtagatgttg
    18121 tgaccattgt acaccacgga tccagttgtg gtcacctctg gattgagatc tcccatgcgg
    18181 ttgagagact tgaggagggt tgacttccct gatccagatg gaccaatcaa ggctgtaatt
    18241 tccttaggtt ggaaagatag ggaaacacta ttcaaagcct tctttttatt ataataaacg
    18301 gacaggtctg atacctgtaa aatcgcatct gtcatacggt ttcctttcta accaaagtga
    18361 ccagatacat agtcattggt ggactgtagc ttggcatttt ggaaaatagt tgcagtcttg
    18421 tcatactcaa tcaaatcacc caagtaaaag aagcctgtat agtcacttgc acgagcagcc
    18481 tgctgcatat tatgcgttac aatgatgatg gtaaagtttt tcttgagctc aaacatggtc
    18541 tcttctagtt gcatggtcgc aatcggatcc aaggctgagg ctggctcatc cattaagagg
    18601 atatctggct taacagagat ggcacgagcg atacagagac gttgttgctg accacctgat
    18661 aaggtcaagg ctgacttgtg gagatcgtct ttaacctgat cccagagggc agcctgacga
    18721 agggaggttt ctacgatttc atctaggact tgcttatcct taactccagc acgttcatgc
    18781 gcaaaggtaa tattacggta aattgactta gcaaatggat tggggcgttg aaaaaccatt
    18841 ccaatgtgtt tacgcatttc ataaacgttg atttctggac ggttgacatc aattccacga
    18901 tagagaatct gcccagttac tttagcaata tcaatagtat cattcatgcg attgagactg
    18961 cgtaagtagg tagatttccc cgatcccgac ggaccaatca aagctgtaat tttatttctt
    19021 tcaaattgca tatcaatccc cttaatggat tcatttttac catagtaaac atggacatcc
    19081 ttagtagaaa gggctacttt ttcttcagga aaggtaagga tatgcttctc atcccagtta
    19141 tatgttgaca tggcttctcc tttaggcagc ggttaatttc ttgtgtagat agcttccgaa
    19201 cttacgagct ccaaagttaa aaatcaggat aaagatcagg agcacagcgg cagaacctgc
    19261 tgatacaatg gttccatctg gaatagtgcc ttcactattg actttccaga tatggacagc
    19321 caaggtttct gcttgacgga agatagagat ggggctagtc acactgagga tattccagtt
    19381 agaccagtca agagctggcg ccgattgccc tgctgtatag atcagagctg cagcttcgcc
    19441 aaagatacga ccagatgcca agacgacacc cgttacaata cctggaagcg cttctggaat
    19501 aacaacatga accactgtct cccagcgaga aatcccaaga gccagaccag cctcacgttg
    19561 ggtatggtga acgtgtttca aactatcctc tacattacgc gtcatctgag gcaagttaaa
    19621 gactgtcaag gccaaggcac ctgaaatgat tgaaaatcca tactcaaact ggactacaaa
    19681 gatcaagtaa ccaaagagac ccaccaccac tgatggtaaa gaggacaaga tttcaataca
    19741 agtccgcaca aagttggtaa caggaccttt tttagcatat tcagccaagt aaatcccagc
    19801 tcccatagaa agaggtacag aaataatcaa ggtaatgacc aataggaaaa aggaattgta
    19861 aagctgaatg ccaatcccac cacctgcttg aaaagcagaa gaccttccag tcaagaaaga
    19921 ccaagagata tggggcaagc cccgaaccaa gatatagaga atcaaggaag ccaagattgt
    19981 cacaatgatg ctagcaatcg tatagaggac agctgttgca agtttatcta atttcttagc
    20041 gcgcataatt tttctttcct ctttctttcg taatcaattt aatcacactg ttaaaaacta
    20101 aactcatcaa gagcagtacc aaggccagtg accagagaac attattattt acagttccca
    20161 tgacagtgtt cccaattccc atagttaata tagaagttaa agttgcagct ggtgtggtca
    20221 aggaagttgg gataacagct gagtttccga caaccatctg gatagctaaa gcctcaccaa
    20281 aggcacgcgc catcccaaag accactgcag tgaaaatacc agaacgggcc gccttcaaga
    20341 tcacacgcca gatagtctgc cagcgagtgg ctcccatagc gaaactggct tcacgataat
    20401 aacgaggaac cgcacgcaag ctatccgttg tcataaaggt tacggtcggc aaaatcatga
    20461 caaagaggac ggaaatccct gacaaaatcc caaaaccagt cccaccaaag acactgcgaa
    20521 caaagggaac gacgacttgc aagccaataa atccgtacac tactgaagga atcccaacca
    20581 ggagttcaat agctggttgc aaaatcttcg ccccttttgg tgatacttcg gtcataaaaa
    20641 ctgctgcacc aatagcaaag ggtgttgcga taagggctga gagaatggta acgataaagg
    20701 aacccaaaat cataggaagg gcaccaaatt ctttactaga aggattccaa gttcctccca
    20761 aaagaaagtc aaagatattc acaccattga caaagaaggt cgacaagcct ttttgcgcta
    20821 cgaaaaccaa aatcatggcc acaaggatga ctatcaaaga aagacaggca aaggtcaaac
    20881 cttttcctaa tttctccaga cgagaattct ttgatggaag caacattttc ttagctaatt
    20941 cttcttgatt cattattgtc tcccttccaa cactgtcaca gttccggcag catctttttc
    21001 aaccttcatt tccttaatcg gaatatactt caatcctttg acaatccctt cttgggtctc
    21061 atccgagaga acaaaattga gaaattctgc agccaactca ttgggctgcc ccaatgtata
    21121 catatgctca taagaccaca agggccaatt attgctactt atattttctg gacttaagtc
    21181 atagccattc aacttcatgc ttttgaccga atcatctata taggtaagag ataaataaga
    21241 gatagctcct ggactttttg atacgattga ttttaccgct ccatttgaat cctgctcctg
    21301 actttgcatg gcagactgac cttccataat gacagtatca aaggtagcac gagagccaga
    21361 gccggctgcc cgattgataa cagagatggg taagtcctta ccaccaacct ctttccaatt
    21421 ggttacctca cctatgaaga tttgacgaag ttgctctgtc gttaggttat caacatcaac
    21481 ctccttattg acaatcagag ccaagccagc taccgcgacc ttgtggtcaa caagagcaga
    21541 agcatcaatt ccgtcttttt cctcagcaaa tacatctgag tttcctatat caactgcccc
    21601 agactgaacc tgggacaagc ctgtaccaga acctccccct tggacattga ccgtttttcc
    21661 aacatgaatc gtgccaaatt catctgccgc tacttcaacc aagggttgca aggcagttga
    21721 gccaacagcc gttatggatt ctccacgatc aatccagcta gcacagccta ctaaacaagc
    21781 cgtcagccaa aaagcgataa gagacagagc aagctttttt ctttttttca ctgtttttct
    21841 cctcgaaaat aattatgaat actgtgaatt ttttaagtag ttctttatga gttgacgcat
    21901 gaattcttac caaatttctg cgcaattgat tatttatata atataggcta tattactctt
    21961 tcctaacctc cttttttcat atgtggataa aatctcttgt ctatcccttc ccccattgtc
    22021 acccattata atcatttcgt gtctcttttt ccccttttta atgcaaggga aattactctc
    22081 ctcagatgat aatccaaaag ctagaaaggt atctcaaacc tctctactct cccagactag
    22141 tttacaacta aaaggaaaag attctatttt atgagaaatc tagtttacaa gcggtaagaa
    22201 cgctaataac taaacttctt gtactctttg aaaatctctt caaaccagtg ttttgagcta
    22261 tctatggcta gcttcctagt ttgctctttg attttcatcg agtagtaaaa ctacatgtga
    22321 tggcaatcaa gatatcaaga atcatcctac taaaaaaatc catactttca ctataacata
    22381 gaataagata tttgactagc attttcattt gaatctgagg ccttttggaa aataattttt
    22441 caaaacattt ccagtaacct ttgcaaagcc caagccattg cctttaacca aaacttggta
    22501 ccaaccattt ggcagacttt ctgccagctg aacggtttct ccagccgcat acttgacaaa
    22561 cgcttcttgg ccaatttcaa ccgactgttc gacctgactc ggtttcaagg ctaaaccaag
    22621 agcgaaactg ggctcaaagc gtttcttctt aaaagtgccc agatgcagtc cattgcgagc
    22681 aatcttgagc ttccctaaat ctggcaaaag ttctggcaag agataaagct ggtctccaaa
    22741 agtctgcaag atacccggta gattgacctt caaatggttt tgggcaaatt cctgccacaa
    22801 ggcaacttgt tcacggctga ggttactctt acttgcctta aatttaggag ctggattgtt
    22861 acccttaaac tgtagatggg caacaaactg accctctccc ttaaactgat gagggtacat
    22921 ccgagccgtt tctggcaggt caataccagc taccattcca ttgatatgct ctactggcaa
    22981 caagtcaaaa tcatactctt ccagcaacca attgacaatc tcttcgtttt cctcgggtgc
    23041 ccaggtacag gtcgaataaa ccagacgacc accttcagct aacatggtca ctgcatcctc
    23101 cagaatttct ctttgcaagc tagcacattg actcggataa tctaagctcc aatagtccat
    23161 agcatcaggt tgcttacgaa acattccttc accagagcaa ggggcatcaa gaacgattaa
    23221 gtcaaaatag cctttaaaga ccttgaccaa gcggtcggca gattcattgg tcaccacgac
    23281 atttgtcgct ccaaaacgct ccatgttttc aaccaaaatc ttagcccgtt tgcttgaaat
    23341 ttcattggaa acaagtagcc cctcacctgc tagataggct gccagttgag ttgatttgcc
    23401 ccccggtgca gcagccaagt ccaagacctt cataccagga ctgggttggg ctacttgagc
    23461 caccatttga gcagcaggtt cttgcgaata aactaaacct gtagcatgct caggcgattt
    23521 ccctgaaacc ttcccatagt ggccccaagg ggtttgagta atggcatcag aaaaggaaag
    23581 ttgctcttct tttaagggat tgacccgaaa ggccgaaacc gcttcctcct caaaagaggc
    23641 aagaaaatct cttgcctcat ctcctagtat ctctttatat ttttcaacaa atccttctgg
    23701 aaattgcatt taagttcttt tcctttcgta aatataggac tgaatttcct cctgcatctc
    23761 aagaggcacc atcatgaccg gctgtctggt ttgaaaatca ggagcttcac caaaaagggt
    23821 cacaacccga tagcccagac tttcccctaa aatactagct gcggcataat cccatggttg
    23881 cagataagtg agataggtca acaaacgccc tgacaaaatc ttggcaaaac taatggccgc
    23941 acttccatag acacgaacac caagaaccga tcggctcaaa tcagccagcc cccattcatt
    24001 ggtttccagc ataccactat tccctgcaat gagaaaatct ccaagtggtt tagttttaaa
    24061 aggagctagg ggcctatcat ttagacaaac tggaaattcc ccaccaccgt ggtaacaatc
    24121 ccctttgacc acatcataaa tcagaccaaa ctgtccctga ccattttcaa aataagccat
    24181 cataacagca aaatcttcct gctgggctac aaaattattg gtaccatcaa tgggatcaat
    24241 gacccaaacc ttgccctctt gaaccgaggc tcgcagacaa ccttcttcag cacaaatctt
    24301 atcctcagga taacgggaca aaatctcacc aaccaagagt tcctgaactt ctttgtccag
    24361 tctggtcacc aaatctgttg aagaggactt ggtttcaaca cgcaagtctt cctgcatatg
    24421 gtcaagaatg tactggcctg ctttcttaac aagctcttta gcaaattcaa atttactttc
    24481 caagagaaat ctttccttcc cctttttctt tggcagcctg aactgctcgg taaagtgaat
    24541 agccactaac cgtttcaaat tctcgtccca aacgtttctc ttcgctcttg cttggcacga
    24601 ccgctttgaa tcccttatag gagtccagta actttttagc ctctactttg ccttcatagg
    24661 cagcttcaac atcattaaaa aaagaaagca ctgaagcaag ttcttcagtg ctccacgaca
    24721 aatctagtgg gtaactatac tgtttgttca ttaactaata ccagctctca ttcttgcttc
    24781 ttttagttct tgcttacgat aactacgagg gagaaaagca cgaatctcat cttcattaaa
    24841 accgatttgc atacgcttgg catcaataat aattggacga cgcaaaagac taggatactg
    24901 ctcaatcaaa tgaagcaatt ccgataccga aatactctct acatcaatat tcaatttttg
    24961 aaaaattttt gaacgagttg aaatgatgtc atcagtacca ttttcggtca aggaaaggat
    25021 gtgttgcaat tcttttcttg ttaaaggact ggtcataata ttgtgttcca caaagggaac
    25081 cttatgtttt tctaaccagg ccttagcctt acgacatgat gtacagctcg gtgatagaaa
    25141 tagtgtaatc atgcttttct cttcttatct atactttgct acttctatta tacaaaaaaa
    25201 taaggcgctt gactagggat ttttagaaaa aaagcctatt ttttcaagaa aaataggctg
    25261 tttgcgaacg attgacacaa ttggatttgg ttaattcact cttaacgatg gttttaaacg
    25321 atatatattt ttatatatgc aaattaaaaa catctttcat ttcacttcct acgacttttc
    25381 agacacagat acccaaagaa gctttcatag agggcaaaaa agaggaggaa ggcatgaaga
    25441 aagaaggtct ctggcaaaat cataataaca ggatccttgg ctggatcaaa aagccaggta
    25501 tcatctccca caaagagaat ttgatggaaa agagtaaaga attggtcaaa accaatcaaa
    25561 actcccccaa gtccaatcat cacaggtaag actactagag tcaggagact ttttcgataa
    25621 agagacaaaa agtccttttt cacaatccta ttgacaaaga catagaaact tggcagtgtc
    25681 actagagcta ctagctgaac caaatgaaag agattcttga ccactgcgaa atggtgcaga
    25741 ccagctgctg acgaacgaaa atcaggcatc tgtaagacct gactaaaagg attggtcaga
    25801 taattcatca agatatgaaa attgtattga atggtttctg gttttagata gactcgattc
    25861 gttaagttta gccactgaat ctccatagga tagaaaatcc aagccagata aatggtcaga
    25921 aggattgaga gggagaggag aaagagcata gagccccaaa aggtcaattt agttttcatc
    25981 aaaatcccac tccgcaaggc tagaaaccac atgtgtcggt gcgattggca ggccagctac
    26041 ttcttctgcc ttggtaaaac ctgtcgtcac caagagtgtt ggaatgccat tgtcaatccc
    26101 agcccggata tcagtcaaat aattgtcccc aaccatgatt aactcttcac gttccaaacc
    26161 taagtgctca accgccttgt ccataatgat ggcatttggt tttccgatat aaaccggctt
    26221 cactcgtgtc gctacttcaa gcagcgtaat cagtgagcca gcacctggca aaagaccacg
    26281 ttccgtcggg atgttgaggt caggattggt tccgataaaa tgggcaccct tttgaatagc
    26341 aagagttgct gtggcaaatt tttcatagtc gacttgccaa tccagaccaa ctaccacgta
    26401 ggcagggttt tccttgtctt ccacataacc agccgccttg atggcttcct tgagtcctgc
    26461 ttctccgacg acatagacgg tcttttcaag ccccaaatca ttcatatagt cgatggttgc
    26521 caaagtcgct gtgtagacag tcgatagagg cgtatcgata ttaaaattct gagccaacat
    26581 ctccttaaca ctctctggag tgcgggttgt attgttggtt acaaagagat agggaatgtc
    26641 ccgcttttgc aattcatgaa caaaagtctc tccagcaggg attcggtctt tccccttata
    26701 aatggttccg tctaaatcga ttaaatagcc tttatatttc atctatttct ccctaaacct
    26761 tttttatttc ttgccaagta atgattgctt gggcattgat aaccccatca cttgtaattt
    26821 catgcttgct ttccagtcca gtccgttcaa cagccgatgt aatcacccca cctggtcgaa
    26881 cttccttgac atacttgagg ttgattttct tgggaatata gtgggtcaaa aaatccgctc
    26941 ccatgacctc aaaaatccag tccaagtatt tactgttatt gacatgacca ttcatatcca
    27001 agtcgtaaaa acgaacatgg taatccttgc tgatcggctc ttccaaggat tcatacttcg
    27061 gtccacggat aagtttttta tcaaaatcag actggtaagg agccacaatc tcaggttcaa
    27121 cagcatggac ttttcgactg tcgcggtcca tgagaacaaa ggtcgccatc atatggatga
    27181 gctcctgccc cgcttcattg taaatagtaa agcgacggta gcaaaaaagt cgattgtagc
    27241 tcaaggcttc cgtttcgatg gtaatttctt ccgcaaaacg aggcaaacga accacctcaa
    27301 tatcatattc tgcgataatc cagaccagat tatagtcttc caaaatggcc ttatcactaa
    27361 ctcccagttc aatcgattgc atccctgaaa cttgcagtga cagcaaaatc acatctggaa
    27421 gtttgatatg accgttcata tcagccatat caaaaggaat tttcattttc atttgataag
    27481 ttaagcccat gatcctactc caaaataaat cgttctgcta cagtatctcc caaaaagaga
    27541 cctctctttg tcatgcgaac gtggtcaccc tcaatctgca tgaggccttg ttgaaccaaa
    27601 tctctgacaa tttctccata aagtccagca aaagactgtc caaatttttc ctcaaatcgc
    27661 gccatggaaa ccccggattt cttgcggagt cccaagaaca tttcttcttc catttgctcc
    27721 ttttgactca ggtgatcttc tgtaatacaa gcattgcctt cctcaaccgc actgagataa
    27781 tgacgaatgg gaccatgatt tttatagcgt actccattga cataaccaga tgcccctgca
    27841 ccaataccat agtattcagc attgtcccag tacatgagat tatgacgact ttcaaaaccg
    27901 ggtttggaga aattagaaat ctcataatgc tcaaaacccg ctcgctccag ctctgcaatg
    27961 atgtactcaa acatctccgc ttctagttcc tccttaggca gaggcaattt cccacgtcgc
    28021 atccggttca taaagaccgt atggttttct aaaatcaaac tatacaaact catgtgggga
    28081 atatccaatc caatggcttt agccacattt tcctttactt gctccatggt ctgaccaggc
    28141 agagcataaa tcaaatcaat ggagatgttg tcaaaaccag ccagtttcag gcgatcgata
    28201 ttttcataaa tatccttctc caaatgactg cgcccaatct ttttcaacat cttatcatca
    28261 aaggtctgga cacctagcga aacacgattg acagccgaat ttttcaaaac agctatctta
    28321 tccgcatcca aatcgcctgg attggcttca atggtcaact cttccaagac agacaaatcc
    28381 aagtttttag tcaagccatt cagtaacacc tccagttgcg gagccgacag ggctgtcggt
    28441 gttccaccac cgatataaag ggttgacaac ttttcaatat cataagaacg aaactcttcc
    28501 agcagatgct ctaaatagct gtcgactggc tgatttttga tgaagacctt tgaaaaatca
    28561 caataataac aaatctgggt acaaaatggg atgtgcacat aggctgacgt tggttttttc
    28621 tgcatagtaa ttattatacc acaaagacta gattccagat aaaaatcacc atccccagat
    28681 acatagtccg tccggagatg gtgatggttt attcttctgt tatatgaatc acaatctctt
    28741 ctgagtcatc aagagcttcg gctttttctt gccattgttc cttgagatta tttaattgat
    28801 tttttgatgc ttctgtcgct tgaaaagcat aggatttagc ttgagcaagt atactgtcca
    28861 cagtgatttc acctgactca acctgttctt ttgttttcag aacaaaatct gtagcctgct
    28921 ccttaacttc tgtcagtttt tcacagactt gctccttggc atactccgga tcttctctca
    28981 aatcatctag aaaatcttga gcctgactgc aaacttgttt gcccttatca cttgttaaaa
    29041 acaaggcaag agctgcacct gaaaccgttc ctaaaaggat tgaggataat ttacccataa
    29101 ggattctcct tttttatttt ttgaaaaatt tacttgcaag acgaagagct gacagacttg
    29161 caccagtctt gagtgttttt gaaccagctg atgaagcttt cttgctcaag acacgcgcat
    29221 ggtcattgag gtctgaaaca gatagagata aatctgcaac agcactgaag agtggatcaa
    29281 tcgtagccac cttgacattg atatcatctg ccaagacatt gaccttagcc aacaactcat
    29341 tggtgtgatg caaggtcaca tccacatctg aagtcaaggt tttaatcgtc ttttctgttt
    29401 catcgatgac acgaccaagc ttttgtacag taatgatcag atagaccaaa aagacaatca
    29461 aagctagggc aacaagaata tatgcaactt ctaacattta gttttcctcc tctgtaatat
    29521 agtaaggggc cttctttcga ttttgataaa taacgatcat tataccgaga ccgataagga
    29581 caactgacag ccattgggac actcgaagtc cgaagaacat gagactatct gttcgcatac
    29641 cttcgatgac catacgaccg aaaccatacc aaatcaagta aaaggccgtg atatgacctc
    29701 gtctgagact cttccatttc cgtctaaaaa tcagaatcaa ggcaaagcca agcagattcc
    29761 atagagactc ataaaggaaa gtcggttgac ggtagctccc ctcaatatac atctggtcac
    29821 ggataaagcc aggtagataa tccagattat ccactgttgc accataagct tcttggttaa
    29881 agaaattacc ccaacgcccc aaactttgag caatcataac gctaggcgcc gcaatatcta
    29941 gaaaatccca agtattgatg agtttacggt cagcaaagat atagagcaca agagccccag
    30001 ttatcaaacc accgtaaatg gccaaaccac cattccaaat ggcaaaaatc tctcctaaat
    30061 tctgactata gtaatcaaat cggaaaataa catagtagag acgagctcct aaaatagcca
    30121 agggaaaggc tactaagata aaatctaaaa tatcgtctgg tatgatcttc tttctaggtg
    30181 cttctttcat ggtcaaataa accgcaagaa tcaagcctgt cacaatacat aaggcatacc
    30241 aacgaatggc taggggtcct agttgaatag caattggatc aagcattttg cacctcattt
    30301 cgagcgatga gacttgtcag tcgttcgtcg aacaaacggg tcgcatcaaa gcccatttcc
    30361 ttggcacgat aattcatggc agctgcctca atcacaacag agatattacg acctgtttta
    30421 actggaatac gaatacgagg aatggctacg ccagaaactt caagttcctc tgcattgttt
    30481 ccaagacgat caaaggtctt atgcgtatcg taattttcca aatagacagc aagctgaacc
    30541 tgtgaagaat ccttgacagc actcgcaccg tagagactca taacatcgat aataccaacc
    30601 ccacgaattt caatcaagtg tttcaaaatt tcagctggtt caccccagag agtaatctca
    30661 tccttggcaa agatatcgac acggtcatcg gctaccaaac ggtgaccacg tttgacaagc
    30721 tcaagacctg tctcactctt accaattcca ctatctccct gaatcaagac gcccatccca
    30781 taaatatcca tcaagacacc gtgcacactg gtacgttctg ccaaacgaga atccagatag
    30841 ctagataact ctccagataa acgactggta gctgcacggc tggttaaaat agcaatctta
    30901 cattctctag cagcctttaa catctcctct ggaaccacca aaccacgggc aacaatgact
    30961 gctggtgtct caggtagaaa catttttttc aaaacttcat aacggctgtt ggaaggcatg
    31021 ctgatcagat aagaccactc cttcatcccc aaaagttgaa tccgctctgg tgtatagtag
    31081 tcaaaatagc ctgtcatttc aagaccaggt cgcgtaatat ccgctatatt gatttccttt
    31141 tcaagcaatt ctggttcacc atagacaatg tctagtctaa gcttttcaat cacttctttt
    31201 actaaaaccg acatcatttc ctccttcaaa tgagttcttc tctctattat atcaaattgt
    31261 aggaggaagt ttcagatttt tgcaggcttt ggagtattta tttaaaagaa aaaggactag
    31321 atttctctag cctttcattt caattagaat tttttacgtt tacgagctac ttctgattta
    31381 cgtttacgtt ttacagaagg tttttcatag aattcacgtt tgcgtgtttc ttgaagagta
    31441 ccagctttag taaccgcacg tttgaaacgg cgaagtgcgt cgtcaagaga ttcattctta
    31501 cgtactactg ttttagacat ttttttcact cccttcaagt tcaagattta ttctatttta
    31561 cacgtaaaag gaataattgt caagaggaaa ctaagttttt tattcttcat aatgattaat
    31621 aatatagttc accatctcct ccatatctgc tttagataaa ggtaattccc agtttgttgt
    31681 ttccatttta agaaaagcat cactgacttt agttattgta tagcaatctt taaaaattgg
    31741 aattttttta gattgttcaa ataagatgcc tattctatca tataagacgt gaagttcttt
    31801 actatttgga tcaatatcaa aagactgtaa agagattaag atgtcatttc ctttatatga
    31861 caaatcaatc cacatgtgat ttgataaatc atatggccta gataatctac tacccgaggt
    31921 gtacttttta tttcctccgc ctcctgaata ttgatttctt gtggtatatg gtttatcttt
    31981 tgacttagta aaaagaaaat tcccctcctt atctttcttt tttgtaatga agcctattat
    32041 actttttctt tcttccgttc tagcactctt atatgcttct tttattttta aaaattcttc
    32101 tttattcatg tgtcctccta aagttaagtt gaagttttat atgtctaaaa ccactcactc
    32161 caaagagcaa gcggttctta tactttttat ttttcgagta agctaagcgc ttcagcatct
    32221 gcgatgatgg ttacatcagg gtgattttgg aggctacttg caggtaggtt ctcagtcact
    32281 gggccagata ctgttccggc aatggcttct gctttcgact caccgtaagc aaaaagaata
    32341 atagacttgg catccaaaat gtttttaatc cccattgaaa tagcttgggt tgggacgtct
    32401 tcaatcttgg taaagaagcg tgcattggct tcgatagtag actggtcaag ttctactaga
    32461 tgcgtttgac tgtcaaatgg agtaccaggc tcattaaagc cgatatgccc attgcgaccg
    32521 attcccaaga tttggaggtc aactggatgg tcagccaaaa tttggttgta gcgttcaact
    32581 tcagcttcag cattgtcctt aacaccacga ggcaagaaac tttctttaaa tggtttttgg
    32641 ttgaacaagt tttcttgcat gaagtaacga taagactgtg gattgtcccc atcaaggccc
    32701 acatactcat caaggttgac actggttaga tttgaaaaat caaggtcact ctcaacaatt
    32761 tccttgtaaa attcaagtgg gctacttcct gtcgcaagtc ctagagtttg agctccatta
    32821 gccaattttt ccttcaaaat ctcaaaagca acttttccac cttgaacttg gttttcaact
    32881 ttaataactt tcatcttttc atcctccatt tctgtctata tttattatat ggtatagacc
    32941 aatttttgtc aagtgaaaac gatttaattt ctaaaaaaag agcgtccaaa cttgaaacgt
    33001 gttataatgg ataggattag aacttagaaa gaatgaacaa atatatgaat acagctgatt
    33061 ttgatttcca cttgccagag gaattgattg cccaaacgcc ccttgaaaaa cgtgatgcct
    33121 ccaaactcct catcgtcaac cgtgagacag gagaaatgca agataaacat ttccactcta
    33181 ttattgatat gctggaacct ggtgatgccc ttgtcatgaa cgacacccga gttctccctg
    33241 cccgcctcta tggtcaaaaa gtggagacag gaggtcatgt ggaacttctc ctccttaaga
    33301 acactagtgg agacgagtgg gaagttctgg ctaaacctgc caaacgcctc aaggtcggta
    33361 ctcgtatcag ctttggtgat ggccgcctca gcgctgtcgt tacagaagaa ttgacccacg
    33421 ggggacgcat tgtccgcttt gaataccaag gaattttcct agaagtcttg gaaagtctgg
    33481 gagaaatgcc tctgccacct tatatccacg aaaaattaga tgaccgtgaa cgttatcaaa
    33541 ccgtctacgc caaggaaagc ggctctgctg cagcaccgac tgctggtctt cacttcacca
    33601 aagaactgct ggcagaaatc caagctaagg gtgttcatct agtctatctg actctccatg
    33661 tcggactcgg aacctttaga cctgtttctg tggataatct ggacgaacac gaaatgcact
    33721 cagagttcta tcaactttct gaggaagctg ctgccaccct tcgctctgtc aaagaaaatg
    33781 gtggtcgcat catcgctgtc ggaaccactt ctatccgcac cttggaaact attggttcca
    33841 agtttgatgg gcaaatccaa gcagattctg gttggaccaa tatctttatc aaacctgggt
    33901 atgagtggaa ggtcgtggat gccttctcaa ccaacttcca tctgccaaaa tcaactctgg
    33961 tcatgttggt ttctgccttt gcaggccgtg aattagtctt agatgcctac caccatgcca
    34021 tccaagaaca ctaccgcttc ttcagttttg gtgacgccat gtttatttat tgagaaagaa
    34081 tttctctaat tcttctaata ccaataaatc gctaagatat tatttcaaag aacatctaca
    34141 attgaaactc tagctagctg tagaagacgc ctagtacatt gaaattaaaa tgcttccccc
    34201 tagcttcgaa aatattgcca tagattgcgt tgactctcca aattgattca tctatatttt
    34261 atttcagctt cctatatttt cttcgctgtt tgtaaatcaa aatgcaagac acatgagtag
    34321 caccatattt gttactctta tctgtcctct caagagacta ttatgagtta tttcagaatc
    34381 attcactact ttgaccctga ctctccttag tctcaaaatc aaagacttat actcttcaaa
    34441 aatctcttca aaccgcgtca acgtcacctt ggattatata tgtgactgac ttcgtcagtt
    34501 ctatctacaa cctcaaagca gtactttgag caacctgcga ctagttttct agtttgctct
    34561 ttgattttca ttgagtatta aacaaaaagt gaacaaatct gaattctaat gtacagaaga
    34621 ctaggcttgt tcactttttt atagtcgcta taagatgacc ttatctatag ctttttatat
    34681 ataattatat attcagacat actattatca attttgtcgc agggaggaat ctgttaacgc
    34741 acccattcac cattatcatt gactctatag ccatctatac ttgtattgac cgctaactca
    34801 cccgatgtat ttacataata ccatttacca ccaacttgga accattgatt gactttcata
    34861 gaaccgttgc tgttgagata gtaccatgaa ccattaactt gtacccaacc tgttgccatg
    34921 gaaccatcag tattataaaa ataccacata ccattttctt gtttccagcc tgttgttgga
    34981 gcaactgctt tagctggttc tactgctaca tctgttcctt ggttagatgt aacagataca
    35041 ggatacgaag gaatagatga ttgctcagga acaacaactt tttcaggttc tctcgtccct
    35101 ctccttatac gtctttttac catctcttta gtaatttgac gagaagtagt ttcttcaatt
    35161 gttccatcac gttcatctac agtatagatt gtagtaagag taatttacca atttctccta
    35221 cttcttctac ttcttgactt ttatcaagag ttgagccatc gagatattct gtttcgattg
    35281 gaatttcttg gacaagaact tggggcttgg ttcctttttt aacaactctt gtttgagagt
    35341 cttctttttg acttaaagta ctctcagtta cttgtccact ctttccatct acatcataag
    35401 ttatcgttgt aactgttttc ccattctttc ctagagtaat ctcttgctcc tgtcctgcag
    35461 aaaggtcatt gtctgcttca tatttagtag caaatggaac aagaacttct tcaaccttgc
    35521 ttttagctgg aactttgata actgtatccg tggcttcttt tctatcaaca gtaacctgtt
    35581 cggtaacatg accagtctct ggattaacat cgtaggtcct tgtcctagtt acatagccat
    35641 cctctccatc aattgtaaca ggattttcac tacggtcttt tgtttcatct ttttcataac
    35701 gaattcgcgt acttgaaatt ttcttgatta ctaccttagg tttagtcgct acttttacaa
    35761 taatatcccc attgtcagcg tcatcatact ctattccctc ttctttatct ctagtatcat
    35821 ctctgacata ttgaatccca tcaacagcat gaacaaaact tgtattcaga ttcctcctaa
    35881 aaataaagtt agcccgatta ccgcagaacc aaaaatcttt ccgagtttac gtaatgcata
    35941 gcgcttatta gtattagatt ttgccattac atcctacttc tagtatagca tcttttctat
    36001 caaacgttaa acaatatatt ttatatatca aatagactta gaatgatata ttgattattg
    36061 aactaacact ttaactatat cgtaatcaat ctcatatata aaggattgca gacatcttat
    36121 ctaaatacat gctaatatat ttagatacaa acattccaac ttgataattt tcactcatct
    36181 ttcatcattc cttatacaac tatgcagtat aaatagaata gttttctcat cagaatgaga
    36241 ctattttaat attagatatt ttgatagaaa aaaacataaa taaaattggt tgaatttaat
    36301 tccctaactc gatagaggat tctattttaa atttaacaac agctaggatt acactataaa
    36361 aagcacatta ccacctgttc ggctggagaa tctagtcgat tagctccctc ttagttacaa
    36421 gcttcaagcg attttttaac taatactaaa ccagattaaa gaagaaacta gaacatctgt
    36481 cttgtactaa tcaatatgct attttaaagt tgcaaataaa cttggaatgc taattattat
    36541 atttacaata aatagtcgac ttcgatagaa acagaaaata gaattcaaca ttcttcccta
    36601 cttataccat aataaataag atccgcaaga tagccttttc tctctacacc attaacaatg
    36661 gtttgtagat aggacattcc agccttttcc atgacacgac ctgaagctgg gttgagactg
    36721 gcataacgtg ctctgacctt ttgaaaacct gcttgagtaa aacaaaagtc caagacagct
    36781 ttcaaggcct ctgtcatcat accatgtccc cagtaagcct tgcctagcac atagccgatt
    36841 tcacagctta aatcagcctc gtctatctta acgatgctga tatctcctat cacttgctct
    36901 gggttttctt ttagacaaat ggcccattta taatagttga gattagtata ggaagcaacc
    36961 caattgcgaa tcgagtttcg agtgatttcg acatcaggat ggggatccca ggtaacatag
    37021 gtcagattct cagcggatga agcccaattt tgaaacatgg cttctgcatc actctccaca
    37081 aatcttctta aaatcaaacg ttctgtctgt aatatttgcg taccgataga tttcatgatg
    37141 ctacctcact tccttatttc tatgctcgta gtaaaaagac tcaactcagt aacagctaga
    37201 aaacgagttt gtcttctaat ttcttgacgc agttgctgca tggtttgaaa tgcgctctag
    37261 caagcttttt caaacctagt catcgttgca ggggtaagac gatgaaatcg aactcttcga
    37321 gttaccgaat ctgcctcatt ttcttatttc taaactcggg gttcaagatc ccccggacct
    37381 tctctcactt tcttatttca agatgagagg ctgaaaacct ccagtggagg tttttcttgc
    37441 tttttaattt cagggcaaga agctaaaaag tcccccggac cttcttcgct ttctcatttc
    37501 taggctcagg gtaaaaacct ccaatggagg tttttactcc caaaagtcat caaatacagt
    37561 gatgggtaag tggcgtttgt gttggccttt gtgccaccag ttttcaatgg tcgcttgagc
    37621 ttctgggctg attgttttgc cttctaggta atcgtcaatc tctgcatagg tgactccaag
    37681 tgcgacttcg tcagctaggc ctggtttgtc ttcttctagg tctgccgttg ggattttttc
    37741 ataaagggct ggctctgcgc caagtttctg caagagttgt tttccttggc gtttattgag
    37801 gcggtaaaga gggagaatat ccgcaccgcc gtcaccaaac ttggtaaaga aacctgtgat
    37861 attttccgcg gcgtggtctg ttccaatgac cgctccgcta tgggaaccag caagggcata
    37921 ctgagcaatc atacggcaac gtgccttgat attccccttg ttgaagtctg aaacaggact
    37981 acctgtcgct tcaactgcag ctgtcatggc atcagctgat tccttgatat tcacaaccaa
    38041 gctgacatct ggctggatga aggctagggc tttttgagca tctgcttcat cagcttgcac
    38101 tccgtatggc aggcggacag cgataaattt gtagctatcg tctcccgttt cagctcgcag
    38161 ttcttccata gctaattgcg ccaaacgtcc tgccaaggtt gaatcttgtc ccccagaaat
    38221 ccctagtaca aaggttttta ggaagggatg ttttttcaga tatcttttta agaaatcaat
    38281 agaacgacgg atttcttcct gggcatcaat cactggtttg acacccagct cttggataat
    38341 cgtttcttgc aaactcattc ttcttctcct tcaccaaggg cttccttgcg catcttatca
    38401 atcaagtcca tcttatcttg ccatacatca cgcgccaaat ccactggata gtgctgcgga
    38461 ttgagcacac gcttatactc atcccacaac ttgtcaaatt ccttacgggc ataatcctga
    38521 atgtcagtca aactaggcaa gttgtaaact aatatacctt ctttgaagat atccaccaag
    38581 agaggaacgg catcaaaatt acgaaccgtc ttcttgatgt atgtataggt cggatggaac
    38641 atcttgattt ctgtcatgtc gctaatatcc acaccatcat aagtgatgta gtcaccttct
    38701 gacttgcctt tttcacgact ggtaatgcgc cacacctgct tcttacctgg cgtcgacact
    38761 ttttcagcat tattagacag cttaatcgta ttgcgcatct gaccagtttc atcttcgatt
    38821 gcaacaatct tgtaaaccgc cccaagagcc ggctggtcat aggctgtaat cagcttggta
    38881 cccacacccc agacatcaat cttggccttt tgcatcttga ggttaaggat ggtattttca
    38941 tctagatcat tagaagcata aatcttagcc tctgtaaatc cagcctcatc cagttgctga
    39001 cggactttct tagaaatgta ggcaatatcc ccagagtcaa tccgcacacc cataaagtta
    39061 atctgatcac ccagctcacg cgccacctga atggcagctg gtacaccgat gcgaagggtg
    39121 tcataggtat ccacaagaaa gacacaattt ttgtgggtcg cagcgtaagc cttgaaagct
    39181 tcatagtcat tgccataaac ctgtaccaag gcatgggcat gggttcccaa aacaggaatg
    39241 tcaaagagct tacccgcacg cacgttgctg gttccattgg cgccaccaat cacagctgcg
    39301 cgtgttcccc agatggccgc atccatttct tgagcccgac gtgtcccaaa ctccatcaag
    39361 ggttcatctt cgataaccga acgaatacga gctgccttcg tcgccaccaa agtctggtag
    39421 ttgacgatgt tcaaaagagc cgtttcgacc aactgacatt gggctagagg tccttccacc
    39481 tgcacaatcg gttcattagc aaaaaccaaa tccccttctt gggcagaacg aacggtcaac
    39541 tccaacttga aattgcgaag gtaatccaag aacgccccat gataaccaag cgactccaaa
    39601 taggctatat cactatctga aaaacgcaag tcttcaagat agttcacaat tctttccaaa
    39661 cctgcaaaaa ccgcatagcc gttcttaaaa ggctgttggc ggaaatacac ctcaaagacc
    39721 gccttcttat tgtgaatccc ttggtcaaag taaacctgca tcatgttgat ctggtacaag
    39781 tccgtgtgca atgtcaaact atcatctgga tacatacttt tcctacttcc ttagctagaa
    39841 acccatgaaa attttcaaga actttcatgt attccaataa attagtacta ttatatcaca
    39901 ttttagctgg attgagaaaa gagtaacaag ctattctcca ctctccaatt catccatatc
    39961 ttgttcaaat tttttctgag cccattcgcc atagctctta agaccaagat tgccaataaa
    40021 gatccacgga aggtaaatga tataagtaat gacccaagca gacaggtatt taaaattcaa
    40081 aggattgtgc tgataaattt ctatgttgaa ttgataattc tgcaacatca aaagagccgt
    40141 aatagccaag gttaggaaaa aacaacccaa aatcgtaaaa tgaaaacgac tatagtaggt
    40201 cactcccaga taacgggcac gattgaaaaa gtaaaatgtc cctatgatga taacgattag
    40261 cagcatatta gaattaaaaa ggcttggtgc taatactgaa atgatataag ataggagcga
    40321 caaagcaatg cagatataga aactttcaga gcccgcttta ttgaacagtt gttcttctct
    40381 ttcgtctagt aattgataat aataaaatct atttttcatc ttcttcctcc caaaatagtt
    40441 ggtctagggt tttccctaaa catctgcaaa tagactggca gagcgagaga ctgggattgt
    40501 attttcccgc ctctattaaa ccaatagtct ggcgtgtcac cccgacagcc tctgccagtt
    40561 gaccttgtgt taaatcacgc tctacccgag ctaattttaa ttttaaattt ttagccacct
    40621 tcgtcctcct tatagtttta atactcatct actcttaaaa aatccaaaac caacacaagc
    40681 tatcagctat ttattataac cccattaatt tgcattccag tattatcttc agccgaaaat
    40741 cagtatttta ggttgtttga aataaacttc ctagtttact cttttatttt tatttagtat
    40801 tagctttagt aagatctttt aaacgaattt ttattgctta tatttcttgt ttagtttagt
    40861 tggtttcata tcactattat ataatgcttt tggaaatgag cctagtataa gtcaaaaacg
    40921 tgttgaatag tgaaagcagg agcataccaa gatgactgat tttttactgt attttaggta
    40981 tgcaaaataa tactaaatga aactaaaatg aatacttctt gcttttcttg agagtgaaaa
    41041 agtacaataa aatgattgta gtgattacca caagatactg actaatagac atcgtatttg
    41101 aagccacaaa tacaggtaaa gaataaaaag gaagaagttc aattgctttg ataaacaaat
    41161 cattactagt attcatcatc atcgtattta atatattagg gataaagagt agtaaagcta
    41221 ctaaaatacc aaataccaag ccacttttca atttgtataa atagaaaatg taagataata
    41281 gaaaataaaa caggactaac aaaattgtat tgactacaat agctactata aaatctaatc
    41341 ctaaatcacc tggaataccc aatttactta ctatgactag taacacaatg ccattcaaag
    41401 cataaataat ggaaactgag atggcatata gaaaattaga caagacgtaa atcatccgat
    41461 tcttctgttg attataaaac aaacttaccg tattgtggga aaaatctcta ctaataactc
    41521 gattagcatg tataacagcc attaacatac cgagcatagc ataaaagttt gaaatatgct
    41581 taataccaag cgatgtctct tgagtcttta gaataaagaa tgtaagcaat atcggcggta
    41641 taaacgagat aagcaaactg agatatatta cgcctgatga atataaattt cttatatttt
    41701 ccttaagaaa attcaattta tttaatttca tgattataac cttcctttat tttgaacgat
    41761 ttaagtaaat atctttaagc gtctcttttt tagtttcaaa atcaactact ttaatagagt
    41821 tttcgttaaa aaatttaaaa agctcactac tttgaatatt cccagacata gtaattctca
    41881 gtccctcttc ctgaacaata tccccaaatt cttgtttggt aatgaaaatg tctctatctg
    41941 tagctgatga aaaagctatc tcaaacaaga aattatgact atcttttcct actttttgaa
    42001 atgtcaaaag cccgctctcc aagaaaagaa ccctctcaca aatttcttca atgtcttcta
    42061 atttatgact cgatattaaa attcccacgt tttcatttaa agctaatttt tttagaaccg
    42121 ctaaaactat ttgtgatgat tcaatatcta aaccattagt cggttcatct aaaatcaata
    42181 tatcaggttt cgtaacgaga gttagaagca aagctaattt ttgttttgta cccaaagaat
    42241 aggtctttac ttttttatta atagactgag tcaaatctaa ctcttggatc aaacttctaa
    42301 atctttcttg attgtagtca acaccatata aatttgacaa atattttaaa ttctctaaac
    42361 ctgttttaga taaaaataat tttggttctt cgattaaata cccaatatta tcactgctta
    42421 taatattacc tgaagtcggt tgattattct gaacaagaat tttcatcaac gtactcttac
    42481 caactccatt tctccctact agaccaacaa tttcaccctt gttaagtgca aaatttatat
    42541 ctttgagaat gattgaatga ccataatgct tacttacatt tataacttac attttttatt
    42601 cctccattaa tattcatggt ttgctctttg attcttattg agtttaaaaa gttgcgcctc
    42661 tttttaacaa cattatatca tatatctaac ttaatgcaat atataaatgt cattttgtaa
    42721 aattttatta acaaaaaact ctctacaaca aaataataga gagttttctg cttattaatt
    42781 tttttcaaaa tatctaaaca tcacttcttt gtttaatttg ataggctttg atacctaagc
    42841 tccgacttta tcacttaaaa aataaaaaag gacagctaat ctcttgcgca tcaagggatt
    42901 tcgccattct ttaatctcgt ttaagtgttg tatatggagc tttgcataat tttttaaaac
    42961 taaacaacta cattaaac
//
LOCUS       DAVKQL010000009        42891 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVKQL010000009 DAVKQL010000000
VERSION     DAVKQL010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 42891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42891
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.009"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            13..489
                     /locus_tag="VW561_000418"
     CDS             13..489
                     /locus_tag="VW561_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9942751.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVATLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAEATA"
     gene            527..832
                     /locus_tag="VW561_000419"
     CDS             527..832
                     /locus_tag="VW561_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9942752.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            880..2355
                     /locus_tag="VW561_000420"
     CDS             880..2355
                     /locus_tag="VW561_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEU9942753.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGMQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLLIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVAFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            2378..2455
                     /locus_tag="VW561_000421"
                     /pseudo
     CDS             2378..2455
                     /locus_tag="VW561_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            2750..9448
                     /locus_tag="VW561_000422"
     CDS             2750..9448
                     /locus_tag="VW561_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU9942754.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEVNQMIEDRKVDFNQNWYF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYHPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKSAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVARVRTLIGKEPKMPKTVGFVYSDGSREKLPVTWSQ
                     VDVSQAGVVTVKGTANGREVEAHVEVLAIAKELPTVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEITEVDKAKLSVAGSRIQMTGQLAGETIHATLVVEEGNAAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFVQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLKAEYEGAKGQVDLTIQANTEKKIAQSIRP
                     VNVVTDLHQKPTLPTTVTVEYDKGFPKALKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRSNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAIRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQVRLLQVGQALELPTKVPVYFTGKDGYETKDLTVEWEEVPAEN
                     LTKAGQFTVRGRVLGSNLVAEVTVRVTDKLGETLSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQGKVQFFA
                     DSGTDAPSKLVLERYVGPEFEMPTYYSNYQAYDADHPFNNPENWEAVHYRADKDIEAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IHLTKEKPVSEKTVAAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQAAVAGLALLGLSAGLVVTKGKKED"
     gene            complement(9697..10554)
                     /locus_tag="VW561_000423"
                     /pseudo
     CDS             complement(9697..10554)
                     /locus_tag="VW561_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(10706..11065)
                     /locus_tag="VW561_000424"
     CDS             complement(10706..11065)
                     /locus_tag="VW561_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEU9942755.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKAFTHNFLPRLGLALSILAIILVFFFLLKKRSFYYPKFIKFFWRTGFL
                     LTLIMYIEMVVELFLMK"
     gene            11157..12114
                     /locus_tag="VW561_000425"
                     /pseudo
     CDS             11157..12114
                     /locus_tag="VW561_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; frameshifted;
                     internal stop; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            12098..12655
                     /locus_tag="VW561_000426"
     CDS             12098..12655
                     /locus_tag="VW561_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9942756.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            12648..12902
                     /locus_tag="VW561_000427"
     CDS             12648..12902
                     /locus_tag="VW561_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942757.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERSVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            12914..14968
                     /locus_tag="VW561_000428"
     CDS             12914..14968
                     /locus_tag="VW561_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU9942758.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNRYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGSFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            15147..16007
                     /locus_tag="VW561_000429"
     CDS             15147..16007
                     /locus_tag="VW561_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU9942759.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            16218..16928
                     /gene="ccdA2"
                     /locus_tag="VW561_000430"
     CDS             16218..16928
                     /gene="ccdA2"
                     /locus_tag="VW561_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU9942760.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYILKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            16948..17514
                     /locus_tag="VW561_000431"
     CDS             16948..17514
                     /locus_tag="VW561_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEU9942761.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVTDFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            17525..18637
                     /gene="msrB"
                     /locus_tag="VW561_000432"
     CDS             17525..18637
                     /gene="msrB"
                     /locus_tag="VW561_000432"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU9942762.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            18860..19597
                     /locus_tag="VW561_000433"
     CDS             18860..19597
                     /locus_tag="VW561_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9942763.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQTENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            19594..21285
                     /locus_tag="VW561_000434"
     CDS             19594..21285
                     /locus_tag="VW561_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9942764.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            21401..21964
                     /locus_tag="VW561_000435"
     CDS             21401..21964
                     /locus_tag="VW561_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEU9942765.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILSSRGIHLAVQGCEHVNRALVVERQVAEQFGLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKS"
     gene            21986..27439
                     /locus_tag="VW561_000436"
     CDS             21986..27439
                     /locus_tag="VW561_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9942766.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAGAVDPVATLALASREGVVEMDGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKTEESPKEEPKSEIK
                     PTDDTLPKVEEGKEASAEPAPVEEVGGEVESKPEEKVAVKPESQLSDKPAEESKVEPP
                     VEQAKVPEQPVQPTQAEQPSTPKESSQEDNSKEDRGAEETPKQEDEQPAEAPEIKVEE
                     PVESKEETVNQPVEQPKVETPAVEKQTEPTEEPKVEVTTVETREEVIPFETKEQEDDT
                     LKRGTRQVVQKGVEGKKQITETYKTIRGEKTNEAPIVKETVIEQPQDEIIKKGTKGLE
                     KPTLQWANTEKDVLKKSATASYTLTKPAGVEIKSIKLALKDKDGQLVKEVTVAENNLN
                     ATLDKLKYYQGYTLSTTMVYDRGEGEETEKLEDKQIQLDLKKVEIKNIKETSLMNVDA
                     EGNETDKSLLSEKPTDVSQLYLRVTTHDNKVTRLAVSSVEEVVVDGKTLYKVVAKAPD
                     LVQRRADDTLSEEYVHYFEKQLPKVNNVYYNFNELVKDMQANPTGEFKLGADLNAANV
                     KPNGKSYVTKPFKGKLLSNDGGRFTIHNIERPLFANIEGGKIHDINLANVNINMPWAD
                     KVAPIANVIKNNATIENVKVTGNVLGKDWVSGFIDKIDGSGKLINVAFIGNVTSVGTG
                     GNFLTGIVGENWKGYVERAYVDAHIKGKRAKAAGIAYWSQNQGNNFTIGSEGAIKKSV
                     VKGTIDVEKPIEVGGAVGSFTYHGSIEDTVSMMKVKNGEIFYGSKDIDDDPYYTGNHV
                     NRNYVVIGVSEGTSTYRYSNQHNRIKPITQSEADVKIAETAITADKFTITDPIVNKLN
                     ALTTRDNEYRTTQDYEATREQAYRNIEKLQPLYNKEWIVNQGNKLVEGSNLLTKEVLS
                     VTGIKSGQFVTDLSDIDKIMIHYSDGAKEELNVTRQESNVQQVREYSITDLDILYTPN
                     MVEKDRAQLMTDVKSKLSSVELESDGVRQLLVKRDTKKDANANSVGRQNGYIRDLFLE
                     ESFSEVKANLDKLVKQILENEDHQLNDNELAERALLKKVEDNKAKIMMGLAYLNQYYA
                     FKYDELSIKDIMMFKPDFYGKTASVIDRLINIGSAENNLKGDRTQDAYRGIISGATGK
                     GSLHDFLTYNMKLFTNETDINVWFKKAIEKNAYVVEQPSTNSAFANKKYRLYEGINNG
                     QHGRMILPLLNLKNAHLFMISTYNTISFSSFEKYGKDTDEKREKFKSEINKRAKEQVN
                     YLDFWSRLATDNVRDKLLKSQNVVPTPVWDNHNSPNGWASRHGHIDGKPDYAPIREFF
                     GRINKYHGYKYGYGAYAYIFAAPQPMDAVYFVMTDLISDFGTSAFTHETTHVNDRMAY
                     YGGHWHREGTDLEAFAQGMLQTPSVSNPNGEYGALGLNMAYERQNDGNQWYNTNPNDL
                     TTRAEIDNYMKGFNDTLMLLDYLEGEAVLNKANQDLNNAWFKKVDKQLRGASTKNQYD
                     KVRDLTADEKNITLNSVDDLVDNNFMTKHGPGNNVYDPTGFGTAYVTVPITAGIYGGN
                     TSEGAPGSMSFKHNTFRMWGYYGYEKGFLNYASNMLKNESRQAGHNTLGDDFIIKKVS
                     DNKFSTLEDWKKAYFKEVVDKAKAGFNPVTIDSTTYSSYDDLKNAFAAAVEKDKATLK
                     NGSVKSDNTVALKEKIYKKLLQQTDSFKTSIFR"
     gene            27500..29221
                     /gene="pabB"
                     /locus_tag="VW561_000437"
     CDS             27500..29221
                     /gene="pabB"
                     /locus_tag="VW561_000437"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEU9942767.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVEEVADLLAQVENYQ
                     TQGYYVVGYVSYEAAPAFEEKLAVHKVPLLGEYLLYFTVHDRVETSPIPLTYEDIDLP
                     SNWQEVTSAEDYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLSANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANRDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRSHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQHLLETGQVEEKVLTLADLTQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            29222..29839
                     /locus_tag="VW561_000438"
     CDS             29222..29839
                     /locus_tag="VW561_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9942768.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALTLELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKTLMVGVSEELKTLDLTDIAGTCSYPTLLICGSKDKPNL
                     SSMKALHRLLTDSQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            29889..30887
                     /locus_tag="VW561_000439"
     CDS             29889..30887
                     /locus_tag="VW561_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9942769.1"
                     /translation="MNKRLFLKMSLVTLPILALFSQPVLAEENIHFSSCKEAWANGYS
                     DIHEGEPGYSAKLDRDHDGVACELKNAPKGAFKAKQSTAIQINTSSATTSGWVKQDGA
                     WYYFDGNGNLVKNAWQGSYYLKADGKMAQSEWIYDSSYQAWYYLKSDGSYAKNAWQGA
                     YYLKSNGKMAQGEWVYDSSYQAWYYLKSDGSYARNAWQGNYYLKSDGKMAKGEWVYDA
                     TYQAWYYLTSDGSYAYSTWQGNYYLKSDGKMAVNEWVDGGRYYVGADGVWKEGQASTV
                     SSSNDSNSEYSAALGKAKSYNSLFHMSKKRMYRQLTSDFDKFSNDAAQYAIDHLDD"
     gene            31014..31973
                     /locus_tag="VW561_000440"
     CDS             31014..31973
                     /locus_tag="VW561_000440"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEU9942770.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDREKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            32070..32909
                     /locus_tag="VW561_000441"
     CDS             32070..32909
                     /locus_tag="VW561_000441"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEU9942771.1"
                     /translation="MTKADTIFKENIECILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(32957..33127)
                     /locus_tag="VW561_000442"
     CDS             complement(32957..33127)
                     /locus_tag="VW561_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEU9942772.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            33248..34132
                     /gene="miaA"
                     /locus_tag="VW561_000443"
     CDS             33248..34132
                     /gene="miaA"
                     /locus_tag="VW561_000443"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEU9942773.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEDIQSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQETLYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTFEEASESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            34125..35363
                     /gene="hflX"
                     /locus_tag="VW561_000444"
     CDS             34125..35363
                     /gene="hflX"
                     /locus_tag="VW561_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEU9942774.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            35356..35979
                     /locus_tag="VW561_000445"
     CDS             35356..35979
                     /locus_tag="VW561_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEU9942775.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGFGRIFSENEEKISDDLLFEIAQIFSHQLVFEESGKIYMKPVKDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            35994..36923
                     /locus_tag="VW561_000446"
     CDS             35994..36923
                     /locus_tag="VW561_000446"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEU9942776.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            36944..37699
                     /locus_tag="VW561_000447"
     CDS             36944..37699
                     /locus_tag="VW561_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU9942777.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(37753..38661)
                     /locus_tag="VW561_000448"
     CDS             complement(37753..38661)
                     /locus_tag="VW561_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU9942778.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            38725..38994
                     /locus_tag="VW561_000449"
     CDS             38725..38994
                     /locus_tag="VW561_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEU9942779.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            38994..39374
                     /locus_tag="VW561_000450"
     CDS             38994..39374
                     /locus_tag="VW561_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9942780.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILRARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            39463..39690
                     /locus_tag="VW561_000451"
     CDS             39463..39690
                     /locus_tag="VW561_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942781.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLDYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(39730..40455)
                     /locus_tag="VW561_000452"
     CDS             complement(39730..40455)
                     /locus_tag="VW561_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEU9942782.1"
                     /translation="MRLDNLLAQEKISRKAMKQALFKGDILVDSCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSISQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     EGNYRPLNQKELQIIKNYLEMSR"
     gene            40617..42458
                     /gene="typA"
                     /locus_tag="VW561_000453"
     CDS             40617..42458
                     /gene="typA"
                     /locus_tag="VW561_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEU9942783.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPIDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            42478..42732
                     /locus_tag="VW561_000454"
     CDS             42478..42732
                     /locus_tag="VW561_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEU9942784.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
BASE COUNT        13170 a         7975 c         9528 g        12218 t
ORIGIN      
        1 aagggaaaac ttatggtatt agattatttc tttgacaaaa accttgtgtt ttgcttagaa
       61 gcggataatc aagaacaact ctttgatcag gttgcaactt tattggaaga acgagaaatt
      121 gtcactccaa cttatcgtga agccttgatc acgcgtgaaa agtcatttcc aactggttta
      181 gatatggaat ttctaggaaa ggacttgcca aatgtagcga ttcctcatac agatattgtt
      241 cataatctag ctgagaaagt ggtggttgtt cgattagaaa aaccagtaac ttttcacaat
      301 atgatagctc ctgataagga agtagaagta tccttgctct tctttatcat caataactca
      361 agttcaagtc aaacaaatat tctggctcag ttgatggact ttttcacagg aaatggacat
      421 cttgaagacc tatcaaaaat ttccgaacca gaaaaacttt atgcttacat tgctgaagca
      481 accgcttaat cttgtctatt aaaaaataaa atcggaggaa atctaaatga ttaaaattct
      541 tgctgcctgc ggtgcaggtg ttaactcaag tcaccaaatt aaaagtgctc tagaagaaga
      601 actttcaaac cgtggttatg atgttcactg tgatgcagtc atggtgaaag atgtaaacga
      661 agaccttatg aaaggttacg atatctttac accaatcgct gcaacagacc ttggttttga
      721 accaggtatc ccagttatcg aagctggacc aatcttattc cgtatcccag caatgagcgc
      781 tccagtattt gacaatattg aagcagctat taaagaacac ggattaagct aattctaatt
      841 tgttaacatt catataacat aaaaaaggga ggaactgtta tggatgcaat ctttgaccta
      901 atcggaaagg ttttcaatcc catcttagaa atgggtggac ctgtcatcat gttaatcatt
      961 ttgacagtat tggctttact ttttggagtg aaattctcca aagcgcttga aggtggtatc
     1021 aaacttgcca tcgctcttac aggtatcggt gctatcatcg gtatgctaaa tggtgctttc
     1081 tcagcatcac ttgcaaaatt cgttgaaaac actggtatgc aattgagtat taccgacgtt
     1141 ggttgggcac cacttgcaac aatcacttgg ggttctgctt ggacacttta cttcttgctc
     1201 atcatgttga ttgtcaacgt agtgatgctt gctatgaaga aaaccgatac acttgatgtc
     1261 gatatctttg atatctggca cttgtctatc acaggtctct tgattaaatg gtatgctgat
     1321 aacaatggtg tgagtcaagg ggtttcactc ttgattgcta cagcagctat cgttcttgtc
     1381 ggtgtgttga aaattatcaa ctctgacttg atgaaaccta catttgatga ccttcttaac
     1441 gccccaagtt catcaccaat gacatcaact cacatgaact acatgatgaa cccagttatc
     1501 atggttttgg ataagatttt tgaaaaattc ttcccaggcc ttgataaata tgactttgat
     1561 gctgctaaat tgaacaagaa aatcggtttc tggggatcta aattcttcat cggtttcatc
     1621 cttggtatcg ttatcggtat tatgggaact ccacatccaa ttgcaggtgt tgcagatgca
     1681 gataaatggc gtcttgttat caaaggatgg ttgtctcttg gtttgactgc cggtgtatct
     1741 ttggaactct tctcacttat cggttcatgg ttcatcgcag ccgtagaacc actatcacaa
     1801 ggtattacaa atgttgctac taaacgtctt caaggacgta aattcaatat cggtcttgac
     1861 tggccattca tcgctggtcg tgctgaaatc tgggcttgtg ccaacgtact tgcaccaatc
     1921 atgttgattg aagcagtgct tctttcaaaa gttggaaatg gtatcttgcc acttgcaggt
     1981 atcatcgcta tgggtgttac tccagctctc ttggttgtaa ctcgtggtaa attgctccgt
     2041 atgattatct tcggaacact cttgttgcca ctcttccttc tttcaggtac ccttattgca
     2101 ccatttgcaa cagaacttgc taaaggtgta ggtgccttcc cagaaggtgt gagccaaact
     2161 caattgatta ctcactctac tcttgaagga ccaatcgaaa aacttcttgg ttggacaatt
     2221 ggtaacacta caactggtga tatcaaagca atccttggtg cagtagcctt ccttgtattc
     2281 tatatcggta tctttgcttg gtacagaaaa caaatgatca aacgtaacga agagtacgca
     2341 gcaaaagcaa aataatgcgc tcctataaaa tataaactca atgaaaatca aagagcaaac
     2401 taggaagcta gccgcaggtt gctcaaaaca cagttttgag gttgtagata agactgacga
     2461 agtcagctca aaacatggtt ttgaggttgt agatgaaact gacgaagtca gctcaaaaca
     2521 tggttttgag gttgtagatg aaactgacga agtcagctca aaacacagtt ttgaggttgt
     2581 agatgaaact gacgaagtca gtaatcatat ctacggcaag gcgacactga cgaagtttga
     2641 attggatttt cgcagagtgt aaattgttga aactaggttg tcataccatg tataccactt
     2701 gggctttggt agtagctaac tgcactaaat ataatataag gagggtttta tggggaaagg
     2761 ccattggaat cggaaaagag tttatagcat tcgtaagttt gctgtgggag cttgctcagt
     2821 aatgattggg acttgtgcag ttctattagg aggaaatata gctggagaat ctgtagttta
     2881 tgcggatgaa acacttatta ctcatactgc tgagaaacct aaagaggaaa aaatgatagt
     2941 agaagaaaag gctgataaag ctttggaaac taaaaatgta gttgaaagga cagaacaaag
     3001 tgaacctagt tcaactgagg ctattgcatc tgagaagaaa gaagatgaag ccgtaactcc
     3061 aaaagaggaa aaagtgtctg ctaaaccgga agaaaaagct ccaaggatag aatcacaagc
     3121 ttcaagtcaa gaaaaaccgc tcaaggaaga tgctaaagct gtaacaaatg aagaagtgaa
     3181 tcaaatgatt gaagacagga aagtggattt taatcaaaat tggtacttta aactcaatgc
     3241 aaattctaag gaagccatta aacctgatgc agacgtatct acgtggaaaa aattagattt
     3301 accgtatgac tggagtatct ttaacgattt cgatcatgaa tctcctgcac aaaatgaagg
     3361 tggacagctc aacggtgggg aagcttggta tcgcaagact ttcaaactag atgaaaaaga
     3421 cctcaagaaa aatgttcgcc ttacttttga tggcgtctac atggattctc aagtttatgt
     3481 caatggtcag ttagtggggc attatccaaa tggttataac cagttctcat acgatatcac
     3541 caaatacctt cacaaagatg gtcgtgagaa tgtgattgct gtccatgcag tcaacaaaca
     3601 gccaagtagc cgttggtatt caggaagtgg tatctatcgt gatgtgactt tacaagtgac
     3661 agataaggtg catgttgaga aaaatgggac aactatttta acaccaaaac ttgaagaaca
     3721 acaacatggc aaggttgaaa ctcatgtgac cagcaaaatc gtcaatacgg acgacaaaga
     3781 ccatgaactt gtagccgaat atcaaatcgt tgaacgaggt ggtcatgctg taacaggctt
     3841 agttcgtaca gcgagtcgta ccttaaaagc acatgaatca acaagcctag atgcgatttt
     3901 agaagttgaa agaccaaaac tctggaccgt tttaaatgac aaacctgcct tgtacgaatt
     3961 gattacgcgt gtttaccgtg acggtcaatt ggttgatgct aagaaggatt tgtttggtta
     4021 ccgttactat cactggactc caaatgaagg tttctctttg aatggtgaac gtattaaatt
     4081 ccatggagta tccttgcacc acgaccatgg ggcgcttgga gcagaagaaa actataaagc
     4141 agaatatcgc cgtctcaaac aaatgaagga gatgggagtt aactccatcc gtacaaccca
     4201 caaccctgct agtgagcaaa ccttgcaaat cgcagcagaa ctaggtttac tcgttcagga
     4261 agaggctttt gatacttggt atggtggcaa gaaaccttat gactatggac gtttttttga
     4321 aaaagatgcc actcacccag aagctcgaaa aggtgaaaaa tggtctgatt ttgacctacg
     4381 taccatggtc gaaagaggca aaaacaaccc tgctatcttc atgtggtcaa ttggtaatga
     4441 aataggtgaa gctaatggtg atgcccactc tttagcaact gttaaacgtt tggtcaaggt
     4501 tatcaaggat gttgataaga ctcgctatgt taccatggga gcagataaat tccgtttcgg
     4561 taatggtagc ggagggcatg agaaaattgc tgatgaactc gatgctgttg gatttaacta
     4621 ttctgaagat aattacaaag cccttagagc taagcatcca aaatggttga tttacggttc
     4681 agaaacatca tcagcaaccc gtacacgagg aagttactat caccctgaac gtgaattgaa
     4741 acatagcaat ggacctgagc gtaattatga acagtcagat tatggaaatg atcgtgtggg
     4801 ttgggggaaa acagcaaccg cttcatggac ttttgaccgt gacaacgctg gctatgctgg
     4861 acagtttatc tggacaggta cggactatat tggtgaacct acaccatggc acaaccaaaa
     4921 tcaaactcct gttaagagct cttactttgg tatcgtagat acagccggca ttccaaaaca
     4981 tgacttctat ctctaccaaa gccaatgggt ttctgttaag aagaaaccga tggtacacct
     5041 tcttcctcac tggaactggg aaaacaaaga attagcatcc aaagtagctg actcagaagg
     5101 taagattcca gttcgtgctt attcgaatgc ttctagtgta gaattattct tgaatggaaa
     5161 atctcttggt cttaagactt tcaataaaaa acaaaccagc gatgggcgga cttaccaaga
     5221 aggtgcaaat gctaatgaac tttatcttga atggaaagtt gcctatcaac cgggtacctt
     5281 ggaagcaatt gctcgtgatg aatctggcaa ggaaattgct cgagataaga ttacgactgc
     5341 tggtaagtca gcggcagttc gtcttattaa ggaagaccat gcgattgcag cagatggaaa
     5401 agacttgact tacatctact atgaaattgt tgacagccag gggaatgtgg ttccaactgc
     5461 taataatctg gttcgcttcc aattgcatgg ccaaggtcaa ctggtcggtg tagataacgg
     5521 agaacaagcc agccgtgaac gctataaggc gcaagcagat ggttcttgga ttcgtaaagc
     5581 atttaatggt aaaggtgttg ccattgtcaa atcaactgaa caagcaggga aattcaccct
     5641 gactgcccac tctgatctct tgaaatcgaa ccaagtcact gtctttactg gtaaaaaaga
     5701 aggacaagag aagactgttt tggggacaga agtagcaaga gttcgtacat taattggtaa
     5761 agaaccaaaa atgccgaaaa cagtaggttt tgtatacagc gatggtagtc gtgaaaaact
     5821 tccagtaact tggtctcaag tcgatgttag tcaagcagga gttgtgactg ttaaaggtac
     5881 agcaaatgga cgtgaggtag aggctcatgt cgaggttcta gcaattgcga aagaattacc
     5941 aacagttaaa cgtattgctc caaatactga cttgaattct gtagacaaat ctgtttctta
     6001 tgttttgact gatggaagtg tacaagagta cgaagtagat agctgggaga ttacggaagt
     6061 agataaagcc aaactttcag tagctggatc acgtattcaa atgactggtc agttagctgg
     6121 agaaactatt catgcaaccc ttgtggtaga agaaggaaat gctgcagcac ctgtagtgcc
     6181 aactgttact gttggaggtg aagcagtaac aggtcttact agtcgacaac caatgcaata
     6241 tcgtactcta tcttatggtg cccaattgcc agaagtcaca gcaagtgctg aaaatgctga
     6301 tgtgacagtt cttcaagcaa gcgcagcaaa cggcatgcgt gcaagcatat ttgttcaacc
     6361 taaagatggt ggccctcttc aaacctatgc aattcaattc cttgaagaag caccaaaaat
     6421 tgctcacttg agcttgcaag tggaaaaagc tgacagtctc aaagaagacc aaactgtcaa
     6481 attgtcggtt cgagctcact atcaagatgg aacgcaagct gtattaccag ctgataaagt
     6541 aaccttctct acaagtggtg aaggggaagt cgcaattcgt aaaggaatgc ttgagttaca
     6601 taagccagga gcagtcactc tcaaagcgga atatgaggga gctaaaggcc aagttgatct
     6661 cactatccag gccaatactg agaagaagat tgcgcaatct atccgtccag taaatgtagt
     6721 gacagatttg catcaaaaac ctactcttcc aacaacagta acggttgagt atgacaaagg
     6781 tttccctaaa gctctcaaag tcacttggca agctattccg aaagaaaaac tagactccta
     6841 tcaaacattt gaagtactag gtaaagttga aggaattgac cttgaagcgc gtgcaaaagt
     6901 ctctgtagaa ggtatcgttt cagttgaaga agtcagtgtg acaactccaa tcgcagaagc
     6961 accacaatta ccagaaagcg ttcggacata tgattcaaat ggtcacgttt catcagctaa
     7021 ggttgcatgg gatgcgattc gtccagagca atacgctaag gaaggtgtct ttacagttaa
     7081 tggtcgctta gaaggtacgc aattaacaac taaacttcat gttcgcgtat ctgctcaaac
     7141 tgagcaaggt gcaaacattt ctgaccaatg gaccggttca gaattgccac ttgcctttgc
     7201 ttcagattca aatccaagcg acccagtttc aaatgttaat gacaagctca tttcctacaa
     7261 taaccaacca gccaatcgtt ggacaaactg gaatcgtagt aatccagaag cttcagtcgg
     7321 tgttctgttt ggagattcag gtatcttgag taaacgctcc gttgataatc taagtgtcgg
     7381 attccacgaa gaccatggag ttggtgcacc gaagtcttat gtgattgagt attatgttgg
     7441 taagactgtc ccaacagctc ctaaaaaccc tagttttgtt ggtaatgagg accatgtctt
     7501 taatgattct gccaactgga aaccagttac taatctaaaa gcccctgctc aactcaaggc
     7561 tggagaaatg aaccacttta gctttgataa agttgaaacc tatgctattc gtattcgcat
     7621 ggttaaagca gataacaagc gtggaacgtc tatcacagag gtacaaatct ttgcgaaaca
     7681 agttgcggca gccaaacaag gacaaacaag aatccaagtt gacggcaaag acttagcaaa
     7741 cttcaaccct gatttgacag actactacct tgagtctgta gatggaaaag ttccggcagt
     7801 cacagcaagt gttagcaaca atggtctcgc taccgtcgtt ccaagcgttc gtgaaggtga
     7861 gccagttcgt gtcatcgcga aagctgaaaa tggcgacatc ttaggagaat accgtctgca
     7921 cttcactaag gataagaact tactttctca taaaccagtt gctgcggtta aacaagttcg
     7981 cttgctacaa gtaggtcaag cacttgaatt gccgactaag gttccagttt acttcacagg
     8041 taaagacggc tacgaaacaa aagacctgac agttgaatgg gaagaagttc cagcggaaaa
     8101 tctgacaaaa gcaggtcaat ttactgttcg aggccgtgtc cttggtagta accttgttgc
     8161 tgaggtcact gtacgagtga cagacaaact tggtgagact ctttcagata accctaacta
     8221 tgatgaaaac agtaaccagg cctttgcttc agcaaccaat gatattgaca aaaactctca
     8281 tgaccgcgtt gactatctca atgacggaga tcattcagaa aatcgtcgtt ggacaaactg
     8341 gtcaccaaca ccatcttcta atccagaagt atcagcgggt gtgatcttcc gtgaaaatgg
     8401 taagattgta gaacggactg ttgcacaagg aaaagttcag ttctttgcag atagtggtac
     8461 ggatgcacca tctaaactcg ttttagaacg ctatgtcggt ccagagtttg aaatgccaac
     8521 ctactattca aactaccaag cctacgacgc agaccatcca ttcaacaatc cagaaaattg
     8581 ggaagctgtg cattatcgtg cggataaaga cattgaagcc ggtgatgaaa ttaacgttac
     8641 ctttaaagct gtcaaagcca aagccatgag atggcgtatg gagcgtaaag ctgacaagag
     8701 cggtgttgcg atgattgaga tgaccttcct ggcaccgagt gaattgcctc aagaaagtac
     8761 gcaatcgaag attcttgtag atggaaaaga acttgctgat ttcgctgaaa atcgtcaaga
     8821 ctatcaaatt acctataaag gtcaacggcc aaaagtctca gttgaagaaa acaatcaagt
     8881 agcttcaact gtggtagata gtggagaaga tagccttcca gtacttgttc gcctcgtttc
     8941 agaaagtgga aaacaagtca aggaataccg tatccacttg actaaggaaa aaccagtttc
     9001 tgagaagaca gttgctgctg tacaagaaga tcttccaaaa ctcgaatttg ttgaaaaaga
     9061 tttggcatac aagacagttg agaaaaaaga ttcaacactg tatctaggtg aaactcgtgt
     9121 agaacaagaa ggaaaaactg gtaaagaacg tatctttaca gcgattaatc ctgatggaag
     9181 taaggaagaa aaactccgtg aagtggtaga agctccgaca gaccgcatcg tcttggttgg
     9241 aaccaaacca gtagctcaag aagctaaaaa accacaagtg tcagaaaaag cagatacaaa
     9301 accaattgat tcaagtgaag ctagtcaaac taataaagcc cagttaccaa atacaggtag
     9361 tgcggcaagc caagcagcag tagcaggttt agctcttcta ggtttgagtg caggattagt
     9421 agttactaaa ggtaaaaaag aagactagaa attttgatac cttctttatc attaaagacc
     9481 aaagaaaaac tcttgtccta aatagaagat aaagagtctg ggacaaaata gttctcaacc
     9541 acaaaaaaag ctagatattt ccaattgcgg aactctagct ttttaatttt gagtcgttct
     9601 cttattgtat agtagattga aactagagta gtacacttct acttctaaaa cattgttaga
     9661 aatcgatttg actgtcctta tcgatttgtc ctgttcttat ttcatttcac tatgttttag
     9721 gaggttattg gccataagta ccaatcccat gtcaattctc acttgacgct tgcctctcag
     9781 attacatctc ttgtaaccca aacaagcctt taactgccca aagacaggtt ccacatcaat
     9841 cttgcgttga gcgaaaatct gtctaccttg gggagataaa agtgcctgac attctttagc
     9901 tttcaagttt tgatagcgtt cgttcatata tagccccctt ttgaggggct gattcaggtt
     9961 cgtcggcata gtaaaccttg atttcctgtt gaaagtctgt ctgtgttttc tgatgtttgg
    10021 tatggtgaaa acgataatac cagccatcag gatgtgtgta gctatcctcc ttgtcattat
    10081 agtgccaatt cgctaagttt ttagctgact gtttataccc tctcttttgt tccttatcaa
    10141 acatggcata tttaatcaga aggggagtga tttcttcttg aaaatagact tgtatctgtt
    10201 cttgaagttt ggcggagaat ttctctgtcg ttttcttcca cacaaaacta tacttgttgg
    10261 cattggcttc aatcttagtt ccgtcaataa acaaacaatc taaggtcact aactcttcca
    10321 tttttcaatt tttcgaccag agaaaatccc ttgtgaatag acaaatagaa gagtagatac
    10381 aagcatttta gggtgataag acgggcgatc aaaggcatga tagaaggcgt agaagtgatg
    10441 ttcctccaag gtattcacca ctttttcaat agtaaagacg agatggtctt gtggtaagaa
    10501 ggaactgatt tctagtggta aagttgtttg atttgtgtta tagtgaatat gcatgggata
    10561 gcctttctaa atggtttatt gtgcaattct attttaccaa gactattgtc ccagactctt
    10621 ttctttgtat aaaattgtat agtgttattg tctatactat tttgtagaga aagcaaaaat
    10681 ccctagaaaa tgcttaggga ttcgactatt tcattaagaa caattcaaca accatctcta
    10741 tatacatgat aagggttaat aaaaatcctg tacgccagaa gaatttgatg aatttaggat
    10801 agtaaaagct gcgttttttc aaaaggaaga aaaagacgag aataatggct aggatcgaga
    10861 gggctagccc cagtctaggg aggaaattat gggtaaaggc tttagctgta atcaggtaat
    10921 attcaaatac caagaccgga aaagccaaat ccgcaaagtt tcgtcctagt ttttttaatc
    10981 taaaaagttt ggttaggata atgcagacga ctaaggtcag tatcaataat aaaatagatg
    11041 ctaatttcat taaaatcata cccatattgt atcataaaaa aaggctaatg gaaacgagaa
    11101 acagaggaat ttataggaaa gtcaggcttt tgagattgtg ggtgattttt gttataatga
    11161 aagttataaa atcttataat accttgaatg attattatcg aaaactcttt ggagaaaaga
    11221 cttttaaagt ccctattgat gcgggatttg actgtcccaa tcgtgatgga actgtggctc
    11281 atggaggctg tactttttgt acggtttcag gttctggaga tactattgta gccccagatg
    11341 cgcctatccg tgagcaattt tataaggaaa ttgactttat gcaccgtaag tggccggatg
    11401 ttcagaagta tctggtttat tttcaaaatt ttaccaacac ccatgaaaag gtggaagtta
    11461 ttcgagagcg ttatgaacag gctatcaatg agccaggtgt ggtaggaatc aatattggaa
    11521 cgcgcccaga ctgtttacca gacgaaacca tcgaatattt ggctgagtta tcggagtgca
    11581 tgcatgtgac ggttgaatta ggcttgcaga ccacttatga agcaacctct gacctgatta
    11641 accgtgtgca ttcttatgaa ttgtaggtgg aaacggtcaa gcgtttgaga aagtatccca
    11701 agattgagat tgtttcccca tttgatcaat ggtttgcctg gtgaaaccca tgagatgatg
    11761 gttgaaaatg tccgccgttg tgtcacggat aacgatattc aagggattaa actgcatttg
    11821 ctccatctta tgacaaatac tcgtatgcaa cgagattacc atgagggacg tttacaattg
    11881 atgagtcagg acgaatatgt cagggtcatc tgtgaccaaa tggaaattat tcccaaacat
    11941 atcgtcatcc atcgaattac aggagatgcg cctagggata tgctgattgg tcctatgtgg
    12001 agcctcaaaa aatgggaagt cctcaacagc attgagatgg agatgcgacg tcgtggaagt
    12061 gttcaaggat gcaaggctgt aaaacaggag tttgaaaatg aaaagaccac ttgagatggc
    12121 acatgatttt ttggctgagg tcgtgacaaa agaggatgtc gtagtggatg cgactatggg
    12181 aaatggtcat gacacgcttt ttttagccaa gctagccaag caagtctatg cctttgatat
    12241 tcagaagcaa gccttggaaa agacccaaga gcgtttgcat caggctggct tgacaaatgc
    12301 ccagttaatc ttgcaaggcc atgagacact ggaccagttt gtgacaaaag ctaaggcagg
    12361 gatttttaat ctgggctatt tgccgtcagc tgataagtct gtcatcaccc gaccgcagac
    12421 aacgattgag gcattagaaa agctatgtgg cttacttgtc aaaggtggac gaattgctat
    12481 tatgatttac tatggtcatg aaggaggcga cctcgagaga gatgctgtct tggattttgt
    12541 gatccagttg aaccaacaag agtacacagc tgccatttac cgaactttaa accaagtcaa
    12601 caacccgccg tttttagtga tgattgaaaa attagagaga tacagacatg gataaacaat
    12661 acctacacga aaagctggat gccatgcgcc agaattttgt tgaatcaacc caccacgaac
    12721 gatcggtggg cgtgctagac caagcacata tgagcaaaaa aatgcttaaa atcaagaaaa
    12781 aattagttgc tcttgaaatg gaacggtgcc agagaaaaat tgagcacaaa gactgttcca
    12841 agattgacca aaaaatcaaa gagcagaagg agatatttga atcctgttgt aaaaaagatt
    12901 aaggaggacg tgcgtggaat tactgattta cctcatccta tttttactgg ttttgattgt
    12961 ctcaagtaca accaataagc tcctgccctt tttgcctctc cctttggtgc agattctttt
    13021 gggaattgtg attggtctct ttttacccaa tactgacttt caccttaata cggagttgtt
    13081 tttggcactg gttatcggac ccttgctttt ccgagaggct gaagaagcag atgttacggc
    13141 tattttaaaa cactggcgaa tcattgttta tctcatattt ccagtgattt ttatctcgac
    13201 cctgagtttg ggtggcttgg cccatcttct ttggttcagc cttcccttgg cagcttgctt
    13261 ggctgttggt gcagcccttg gtcctacgga cttggtggcc tttgcctctc tttcggagcg
    13321 ttttagcttt cctaagcgcg tgtccaatat tcttaagggc gaaggactct tgaatgatgc
    13381 ttctggtttg gtggcttttc aggtagcttt gacagcttgg acaactggag ctttttctct
    13441 ggggcaagct agcagttcgc tcatcttttc aatcctaggc ggttttttaa ttggattttt
    13501 aacagccatg accaaccgct tcctccatac cttcttgcta agtgtgcgcg caacggatat
    13561 tgccagtgaa cttttattag aattgagttt gcctctagtg accttctttc tggcagaaga
    13621 agtccatgtt tcagggatta ttgccgtcgt agttgctgga attttaaagg caagtcgctt
    13681 caagaaaatc acgctcctcg aagcccaagt ggatacggtg accgagacgg tctggcatac
    13741 agtgaccttt atgctcaacg gttctgtctt tgtgatttta gggatggagt tggaaatgat
    13801 agcagaacct atcttgacca atccaatcta taatcctcta cttttattgc tatctctcat
    13861 cgcccttacc tttgtcctct ttgtcattcg ttttattatg atctatggct attatgccta
    13921 tagaacccga cgcctcaaga aaaagctaaa tagatatatg aaggacatgt ttctcttgac
    13981 cttttcaggt gttaagggaa cggtgtcgat tgctacgatt ctcttgatac caagtaattt
    14041 agaacaggag tatcctctct tgcttttcct tgttgcaggt gtgacgcttg tcagcttttt
    14101 aacaggtctc ttggtcttgc ctcatctttc tgatgaagag gaagaaagca aggattatct
    14161 catgcatatc gccattttga atgaagtaac gctagagttg gaaaaagagt tggaagacac
    14221 cagaaataaa cttcccctct atgcggctat tgacaattat catggacgta ttgaaaatct
    14281 cattttaagc caagaaaacc aggatgatca agaagattgg gctgctttga aactcttgat
    14341 tcttagtatt gaaagtgatg gtttggagca ggcctatgaa gaggggaaca ttagcaatcg
    14401 tgcttaccga gtttaccaac gttatctgaa aaatatagaa caaggaatca atcgtaaact
    14461 tgcctcaaga ctgacctatt atttccttgt ttctttgagg attttacgtt ttctccttca
    14521 tgaagttttt actcttggaa agaccttccg tagctggaag gacaaggagc aaagccgtct
    14581 tcgtgctctt gattatgacc aaattgcaga gctctatctt gccaatacag agatgattat
    14641 tgaaagtttg gaaaacctga agggagtcta cagacgctct ttgattagtt ttatgcagga
    14701 gtctcgtctt cgagaaacag ctattatcag cagtggttcc tttgtcgaac gggttatcaa
    14761 tcgtgtcaaa cccaacaata tcgatgaaat gctgagaggc tattatctgg agcgcaagtt
    14821 gattttcgaa tacgaagaaa aacgattaat tacaactaag tatgccaaga aattacgaca
    14881 aaatgtaaat aacttagaga actattcctt gaaggaagct gccaataccc tgccgtatga
    14941 tatggtggaa ttggtaagaa gaaattagtt aatactcttc gaaaatctct tcaaaccacg
    15001 tcagcgtcgc cttggattat atatgtgact gacttcgtca gtcttatcta caacctcaaa
    15061 gcagggcttt gagcaacctg cggctagctt cctagtttgc tctttgattt tcattgagta
    15121 taagattata agtgaaggag tgtgacatga aaaagtggtg gaaagagctg atagacaagc
    15181 ctttattaaa agcttttttg cattattatc aagcatcaga tagtgagttg accagtgtcg
    15241 cagtagccta ctattggttg atttcgattt tccccctgct tttggtggtg gtcaatatcc
    15301 tcccctattt tcagattcct gtgggagaat ttctgggctt tatgaaagac gtcctgcccc
    15361 caagcctcta tgaaggtgtg gaaaaaatag ccagagaagt cttgacgcaa ccttcaacag
    15421 gtttattgag tttttctgtt ttatcggcgt tatggagttt ttcaaaatct atgaatttct
    15481 tgcaaaaagc ttttaacaag gcttatggcg tcgaaaagag tcgtggactt atttcccatc
    15541 agatgctaag tctcctagtc agttttggct tgcagctcct ctttgcgttt gccttgtttt
    15601 tgatcttatt tggtcagatg attttggctt tacttgctca ttattggaca aaagatggca
    15661 ttatctatca ggctttacaa gggttggcag gtcctctgat ttacgccctc ctctttgcta
    15721 tcttggtcat gctttattat tttcttccca atctatctaa caggaaaatt agctataccc
    15781 taccaggcag tgcctttgtt ctcttagtga ttttaggttt actaactctg ttctctagtt
    15841 atctcaatta ctatgtccat catttggtgg atgtccgaat tttaggttcc gttcttcttg
    15901 ttgttatgat gttttggttt atcttgattg ctaaaattgt tatcctaggc gcggttatca
    15961 atgccagtat gcagagtttg aaagatccgg tctttaaaaa agactaacta tttatccatt
    16021 ataaaagcgc atagtatcaa gactttatag aaccttgata ctatgcgctt ttgtgctttg
    16081 acagtttttt gtatagtttt tgtccaagat ttctaaatca tctgattaaa ttcctatttt
    16141 cccctatcat tgtccctgtt ttttcgaaaa tggaaggagt ataataaatc tatcaataaa
    16201 atgataggag gaactttatg ggtcatattt tcttttttct aagtgtcttt ttggcaggga
    16261 ttctatcctt cttttctcct tgtatcttac ctttgttacc ggtctataca ggagtgttac
    16321 tagatgataa ggatggtgct caggcttcta gcggcaaatt ttcaatctca gttactagtt
    16381 tattacgaac tctggccttt atagcaggaa tttcctttat atttattttg ttgggctatg
    16441 gagctggttt tttaggcgat ttgctttatg cttcttggtt ccaatatctt actggggcaa
    16501 ttattatcct tcttggtttg caccaaatgg agattctaca ctttaagggg ctttataagg
    16561 aaaagagact acaactgcaa ggacaggggc aaaatggtaa gggctatagt caggcatttt
    16621 tattgggctt gacctttagt tttgcttgga cgccttgcgt ggggccggtt ctggggtctg
    16681 ttttggcctt ggcggcttca ggtggttcag gagcttggca gggagctggt ctcatgttgg
    16741 tgtatacgct gggcttggcg ctaccattct tgcttctagc tctgacctct agttatattt
    16801 tgaaacattt ccgaaaactt catccctatc tcggaatcct caaaaaagtg ggtggttttc
    16861 tcattattgt gatgggactc ttggttctgt ttggaaatgc ttcaatttta agtcaattat
    16921 ttgaataaaa tggaaaggaa tatcaatatg aaaaaatggc aaacatgtgt tcttggagca
    16981 ggttcgctcc tttgtttgac ggcttgttca ggcaagtccg tgactagtga acaccaaacg
    17041 aaagatgaaa tgaagacgga gcagacagct agtaaaacaa gcgcagctaa agggaaagag
    17101 gtgactgatt ttgaattgat gggagtagat ggcaagacct accgtttatc tgattacaag
    17161 ggcaagaaag tctatctcaa attctgggct tcttggtgtt ccatctgtct ggctagtctt
    17221 ccagatacgg atgagattgc taaagaagct ggtgatgact atgtggtctt gacagtagtg
    17281 tcaccaggac ataagggaga gcaatctgaa gcggacttta agaattggta taagggattg
    17341 gattataaaa atctcccagt cctagttgac ccatcaggca aacttttgga aacttatggt
    17401 gtccgttctt acccaaccca agcctttata gacaaagaag gcaagctggt caaaacacat
    17461 ccaggattca tggaaaaaga tgcaattttg caaactttga aggaattagc ctaggaggcg
    17521 tcttatgaat gataagttaa aaatcttctt gttgctagga gtattttttc tagccataac
    17581 cggtttctat gttctattga tacgaaatgc agggcagaca gatgcctcgc aaattgaaaa
    17641 ggcggcagtt agccaaggag gaaaagcagt gaaaaaaaca gaaattagta aagacgcaga
    17701 cttgcacgaa atttatctag ctggaggttg tttctgggga gtggaggaat atttctcacg
    17761 tgttcccggg gtgacggatg ccgtttcagg ctatgcaaat ggtagaggag aaacaaccaa
    17821 gtacgaattg attaaccaaa caggtcatgc agaaaccgtc catgtcacct atgatgccaa
    17881 gcaaatttct ctcaaggaaa tcctgcttca ctatttccgc attatcaatc caaccagcaa
    17941 aaataaacaa ggaaatgatg tggggaccca gtaccgtact ggtgtttatt acacagatga
    18001 caaggatttg gaagtgatta accaagtctt tgatgaggtg gctaagaaat acgatcaacc
    18061 tctagcagtt gaaaaggaaa acttgaagaa ttttgtggtg gctgaggatt accatcaaga
    18121 ctatctcaag aaaaatccaa atggctactg ccatatcaat gttaatcagg cggcctatcc
    18181 tgtcattgat gccagcaaat atccaaaacc aagtgatgag gaattgaaaa agaccctgtc
    18241 acctgaggag tatgcagtta cccaggaaaa tcaaacagaa cgagctttct caaaccgtta
    18301 ctgggataaa tttgaatccg gtatctatgt ggatatagca actggggaac ctctcttttc
    18361 atcaaaagac aaatttgagt ctggttgtgg ctggcctagt tttacccaac ccatcagtcc
    18421 agatgttgtc acctacaagg aagataagtc ctacaatatg acgcgtatgg aagtgcggag
    18481 ccgagtagga gattctcacc ttgggcatgt ctttacggat ggtccacagg acaagggcgg
    18541 cttacgttac tgtatcaata gcctctctat ccgctttatt cccaaagacc aaatggaaga
    18601 aaaaggctac gcttatttac tagattatgt tgattaatac tcttcgaaaa tctcttcaaa
    18661 ccacgtcagc gtcgccttac cgtatgtagg gttactgact tcgtcagttt tatttacaac
    18721 ctcaaagcgg tactttgaga aacctgtggc tatcttccta gtttactttt tgatttttat
    18781 tgagtataag aggtctttcc taagcagtta gaggagaatt ttgctatact gatactagta
    18841 agtggtaaag gagcagatca tgacctacac aatcttaatc gtagaagatg aatatctggt
    18901 aagacaaggt ttgactaaac tggtcaatgt agcagcctac gatatggaaa tcatcggtca
    18961 gactgaaaat ggaaggcagg cttgggaatt gatccaaaag caggtgccag atatcatttt
    19021 aaccgatatc aacatgcctc atctaaatgg catccagttg gccagtctgg tacgagaaac
    19081 ctatcctcag gttcatttgg tctttttaac aggttacgat gattttgatt atgccttgtc
    19141 tgctgtcaaa ctaggtgtgg acgactacct gctcaaaccc ttttctcgtc aggatattga
    19201 ggaaatgttg gggaaaatca aacaaaaact agacaaggaa gagaaagaag agcagttaca
    19261 agatttattg actaataggt ttgaaggaaa catggcccag aaaatccagt ctcatctggc
    19321 tgatagccaa tttagtttaa agtctttagc cagtgactta ggttttagtc cgacctatct
    19381 gagttccttg attaagaaag agttgggctt gccttttcag gattatctgg tgagagaacg
    19441 tgttaaacaa gccaagctct tgcttttaac tacagatctg aagatttatg agatcgcaga
    19501 gaaggttggt tttgaagata tgaactattt tacccaacgt tttaagcaga ttgcaggtgt
    19561 gacacctcgt cagtttaaga agggagaaga ccgatgaagc gttcttctct tttagttaga
    19621 atggttattt ccatctttct ggtctttctc attctcctag ctctggttgg aactttctac
    19681 tatcaatcaa gttcttcagc cattgaggcc accattgagg gcaacagcca aacgaccatc
    19741 agccagacta gccactttat tcagtcttat atcaaaaaac tagaaaccac ctcgactggt
    19801 ttgacccagc agacggatgt tctggcctat gctgagaatc ccagtcaaga caaggtcgag
    19861 ggaatccgag atttgttttt gaccatcttg aagtcagata aggacttgaa aactgttgtg
    19921 ctggtgacca aatctggtca ggtcatttct acagatgaca gtgtgcagat gaaaacttcc
    19981 tctgatatga tggctgagga ttggtaccaa aaggctattc atcagggagc tatgcctgtt
    20041 ttgactccag ctcgtaaatc agatagtcag tgggtcattt ctgtcactca agaacttgtt
    20101 gatgcaaagg gagccaatct tggtgtgctt cgtttggata tttcttatga aactctggaa
    20161 gcctatctca atcaactcca gttggggcag cagggctttg ccttcattat caatgaaaac
    20221 catgaatttg tctaccatcc tcaacacaca gtttatagtt cgtctagcaa aatggaggct
    20281 atgaaaccct acatcgagac aggtcagggt tatactcctg gtcacaaatc ctacgtcagt
    20341 caagagaaga ttgcaggaac tgattggacg gtacttggcg tgtcatcatt ggaaaagtta
    20401 gaccaggttc ggagtcagct cttgtggacc ttgcttgggg ccagtgtcac atctcttctt
    20461 gtctgtctct gcttagtgtg gttcagtctt aaacgctgga ttgctccttt gaaggatttg
    20521 agagaaacca tgttggaaat tgcttctggt gctcaaaatc ttcgtgccaa ggaagttggt
    20581 gcctatgaac tgagagaagt aactcgccaa tttaatgcca tgttggatca gattgatcag
    20641 ttgatggtag ctattcgtag ccaggaagaa acgacccgtc agtaccaact tcaagccctt
    20701 tcgagccaga ttaatccaca tttcctctat aacactttgg acaccatcat ctggatggct
    20761 gaatttcatg atagtcagcg agtggtgcag gtgaccaagt ccttggcaac ctatttccgc
    20821 ttggcgctca atcaaggcaa ggacttgatt tgtctctctg acgaaatcaa tcatgtccgc
    20881 cagtatctct ttatccagaa acaacgctat ggagataagc tggaatacga aattaatgaa
    20941 aatgttgcct ttgataattt agtcttaccc aagctggtcc tacaacccct tgtagaaaat
    21001 gctctttacc atggcattaa ggaaaaggaa ggtcagggcc atattaaact ttctgtccag
    21061 aaacaggatt cgggattggt catccgtatt gaggatgatg gcgttggctt ccaagatgct
    21121 ggtgatagta gtcaaagtca actcaaacgt gggggagttg gtcttcaaaa tgtcgatcaa
    21181 cggctcaaac ttcattttgg agccaattac cagatgaaga ttgattctag accccaaaaa
    21241 gggacgaaag ttgaaatata tataaataga atagaaacta gctaactccc agtcaattct
    21301 gggagtttta tgcgtaattg ggcaaacttt ctaggttgtc tatcttgcta aaaacaagaa
    21361 aaaacggtat gatagaagag tgacaacaga ggtaacagag atgaacgaga cacagattca
    21421 aagagaaaca cgtcaggtag tggaagatgt tttagaaaag accaatttga agcagggggc
    21481 cctttttgtt ttaggtctat cttctagtga ggtgctaggt ggtcagattg ggaaagaatc
    21541 cagtcaagag attggtgagc tgatagtgga gactatcttg ggtattctaa gtagtagagg
    21601 tatccattta gcagttcaag gttgtgagca tgtcaatcgg gctctcgttg ttgaacgtca
    21661 ggtggcagag cagtttggtc tggaaattgt cagtgtccat cctactcttc atgcaggagg
    21721 ttcggggcag ttggcagcct ttaagtttat gcaggatcca gttgaggttg aatttatcaa
    21781 ggctcatgct ggattggata tcggagacac tgcaattggc atgcatgtca agcatgttca
    21841 ggttccgatt cgccctattt tgagagagat tggtcatgcc catgtaacgg cactggctag
    21901 tcgtccaaaa ttaatcggag gtgcgcgtgc gcactatccg caagacgcta ttagaaagtc
    21961 ttgagaatga ataaaggaga taaaagtgtt taaaaaagac cgtttttcaa ttcgtaagat
    22021 taagggagtt gtaggctctg tatttcttgg aagccttttg atggctcctt ctgtagtgga
    22081 cgcagccacc tatcactatg taaataaaga gattatttca caagaagcta aagatttaat
    22141 tcagacagga aagcctgaca ggaatgaagt tgtatatggt ttggtgtatc aaaaagatca
    22201 gttgcctcaa acagggacag aagcatctgt tttgacagct tttggtttgc tgactgttgg
    22261 gagcttgctt ttaatctaca agagaaagaa aattgctagc gtctttctag ttggagctat
    22321 gggattggta gttcttccta gtgcaggggc tgtagaccca gttgcgaccc tagcgctggc
    22381 tagtcgagag ggtgttgttg aaatggatgg ctatcgctat gttggttatc tatcaggtga
    22441 catcctcaaa acgcttggct tggacactgt tttagaagaa acctcagcaa aacctggaga
    22501 ggtgactgta gtcgaagttg agactcctca atcaacaaca aatcaggagc aagctaggac
    22561 agaaaaccaa gtagtagaga cagaggaagc acctaaaaca gaagaaagtc caaaggaaga
    22621 accaaaatcg gagataaaac ctactgacga cacccttcct aaagtagaag agggaaaaga
    22681 agcttcagca gaaccagctc cagttgaaga agtaggtgga gaagttgagt caaaaccaga
    22741 ggaaaaagta gcagttaagc cagaaagtca actatcagac aaaccagctg aggaatcaaa
    22801 agttgaacca ccagtagaac aagcaaaagt cccagaacaa cccgtgcaac ctacacaagc
    22861 tgagcaacca agtacaccaa aagaatcatc acaagaagac aattctaaag aagatagggg
    22921 agcggaagag acaccgaaac aagaagatga acagccagca gaagccccag aaatcaaggt
    22981 tgaagaacca gtagaatcaa aagaggagac tgttaatcaa cctgttgaac aaccaaaagt
    23041 ggaaacgcct gctgtagaaa aacaaacgga accaacagag gaaccaaaag ttgaagtaac
    23101 aacagttgaa acaagagaag aagtcattcc ttttgaaaca aaagaacaag aagatgacac
    23161 tttaaaacgc ggaacaagac aagtagttca aaaaggtgta gaaggtaaga aacaaattac
    23221 tgaaacttat aaaacaattc gtggagaaaa aacaaatgaa gctccaatag ttaaggaaac
    23281 agtaattgaa caacctcaag atgagattat caaaaaaggt acaaaagggt tagaaaaacc
    23341 aacattacaa tgggctaata ctgaaaaaga tgtcttgaag aagagtgcta cagctagtta
    23401 tacattgact aaaccagctg gtgtagaaat taaatcaatt aaattagcgc taaaagataa
    23461 agatggacaa cttgttaaag aagtaacggt agctgaaaat aatttaaatg ctactttaga
    23521 taaattgaaa tattatcaag gttacaccct atctactaca atggtttatg atcgtggaga
    23581 aggtgaagaa actgagaaat tagaagataa acaaattcaa ttagacctta aaaaagttga
    23641 aatcaaaaac attaaagaaa caagcttaat gaatgttgat gctgagggta acgaaactga
    23701 taagagctta ttatctgaaa aaccgacaga tgtttcccaa ttgtatttac gtgtgacaac
    23761 tcatgacaac aaagtgacaa gacttgcggt aagttcagtt gaagaagttg ttgttgatgg
    23821 aaaaacattg tacaaagttg tagccaaagc tccagactta gttcaacgta gagctgatga
    23881 tacactgagt gaagaatatg ttcattattt tgaaaaacaa ttaccaaaag taaataatgt
    23941 atactacaac ttcaatgaac ttgtaaaaga tatgcaagct aatccaacgg gtgagtttaa
    24001 acttggtgca gacttgaatg cagcaaatgt aaaacctaac gggaaatctt atgtaacgaa
    24061 accatttaaa gggaaattac taagtaatga cggcggtaga tttacaatcc ataatataga
    24121 acgtccatta ttcgctaata ttgaaggtgg taaaatccac gatattaatc ttgctaatgt
    24181 aaatattaat atgccttggg ctgataaagt tgctcctatt gcaaatgtaa tcaaaaataa
    24241 tgcaaccatt gaaaatgtca aagttactgg taatgtcctg ggtaaagact gggtatctgg
    24301 atttatagac aaaattgacg gcagtggtaa attaattaat gttgcattta tcggtaatgt
    24361 tacatctgtt ggtactggag gcaacttctt aacaggtatt gtgggagaaa actggaaagg
    24421 atacgttgaa agagcatatg ttgatgctca tattaaaggt aagcgagcta aagctgcggg
    24481 tatagcctac tggtcacaaa accaagggaa taactttact attggatctg agggggctat
    24541 taaaaaatct gttgttaaag gtacaataga cgttgaaaaa ccaattgaag ttggtggcgc
    24601 tgttggaagt tttacttatc atggttctat tgaagatact gtttctatga tgaaagttaa
    24661 gaacggtgaa attttctatg gttctaaaga tattgatgat gatccatatt acacaggtaa
    24721 ccatgttaat cgaaactacg ttgttatagg tgttagcgaa ggtacttcta catatagata
    24781 ttctaatcag cataatcgta ttaaacctat aacacaatca gaagctgatg ttaaaattgc
    24841 agagacagct atcacagcag ataaatttac aattaccgat ccaattgtaa ataaacttaa
    24901 cgctctcaca acacgtgata atgagtatag aacaacacaa gattacgagg cgactcgcga
    24961 acaagcttat cgcaatattg aaaaacttca accgctctat aacaaagaat ggattgtcaa
    25021 ccaaggtaac aaacttgtag aaggttcaaa cttgttgaca aaagaagtat tatctgttac
    25081 aggtataaag agcggtcaat tcgtaactga tttgtcagat attgataaaa ttatgattca
    25141 ctattctgat ggagcaaagg aagaattgaa tgttactcgt caagaatcaa acgttcaaca
    25201 agtgcgtgag tacagtatta cagatttaga tatactctat acaccaaata tggttgagaa
    25261 agatcgcgct cagttgatga ctgatgtaaa gtctaagcta tctagtgtag aattagagtc
    25321 tgatggagtg cgtcaacttc tcgttaagag agatactaag aaagatgcga atgcaaatag
    25381 tgtaggtcgt caaaatggat acattagaga tttattcctt gaagaaagtt tctctgaagt
    25441 taaagcaaat cttgacaaat tggtaaaaca gatacttgaa aatgaagatc atcaactcaa
    25501 cgataacgaa ttagctgaac gtgctttact gaagaaagtt gaagataata aagctaaaat
    25561 catgatggga ctggcatact taaatcaata ttatgctttt aaatacgatg agttatcaat
    25621 taaagatatt atgatgttta aacctgattt ttatggtaag acagcaagtg ttattgatag
    25681 attgattaat attggttctg cagaaaataa tctaaaagga gatagaacgc aagatgctta
    25741 tcgtggtata atttcagggg caacaggtaa aggtagttta catgacttct tgacctacaa
    25801 tatgaaatta ttcacaaatg aaacagacat aaatgtttgg tttaagaaag ctattgaaaa
    25861 gaatgcttat gtagttgaac aaccatcaac gaactctgca tttgccaaca aaaaatatcg
    25921 tctatacgaa ggaattaata atgggcaaca tgggcgcatg attttacctc ttcttaactt
    25981 gaaaaatgca catctattta tgatttcaac atacaacact atctcgttta gttcgtttga
    26041 aaaatatggt aaagacacag atgaaaaacg tgaaaagttc aagagtgaga tcaataaacg
    26101 tgctaaagag caggttaatt acttagattt ttggtcacgt cttgctactg ataatgtccg
    26161 tgataaactt cttaagagtc aaaatgtagt tccaactcct gtatgggata atcataatag
    26221 cccgaacgga tgggctagta gacatggtca tatagatggg aaaccagatt atgctccaat
    26281 tcgtgagttc tttgggcgaa tcaataagta tcatggttac aaatatggat atggcgcata
    26341 tgcatatatt tttgcagcac ctcaaccgat ggatgcagtt tatttcgtta tgacagattt
    26401 aattagtgac tttggaacat ctgcatttac acatgaaact actcacgtta atgatcgtat
    26461 ggcttactat ggtggtcatt ggcatcgtga aggtacggat cttgaagcat ttgctcaggg
    26521 gatgttacaa acgccatcgg tatcaaatcc aaacggagaa tatggcgcat taggtctaaa
    26581 tatggcgtat gaacgtcaaa atgatggaaa tcaatggtat aacacaaatc caaacgacct
    26641 tacaactcgt gcagaaattg ataactacat gaaaggtttc aacgacacat taatgcttct
    26701 tgattacctt gaaggagaag ctgtcctcaa taaagcaaat caagatttaa acaacgcttg
    26761 gttcaaaaaa gtggataagc aacttcgtgg agctagtacg aagaaccagt atgataaagt
    26821 tcgtgattta acggcagatg agaaaaatat cactctaaat tcagtagatg atttagttga
    26881 taataatttt atgacgaaac acggacctgg aaacaatgta tatgatccaa ctgggttcgg
    26941 aacggcctac gtgactgtac ctattactgc aggtatttat ggaggtaaca caagtgaagg
    27001 tgctcctggt tctatgtcat tcaaacataa cacattcaga atgtgggggt actacggata
    27061 tgaaaaagga ttcttaaact atgcttctaa catgctgaaa aatgagtcta gacaggctgg
    27121 tcacaatact ctaggtgatg atttcatcat taagaaagtt tctgataata aattcagtac
    27181 attggaagat tggaagaaag catacttcaa agaagttgta gataaagcta aagcaggatt
    27241 taaccctgtt acaatcgaca gtactacata tagtagttac gatgacttga agaacgcatt
    27301 tgctgcagca gttgaaaaag ataaagctac tcttaaaaat ggatcagtta aatctgacaa
    27361 tactgttgca cttaaagaga aaatatacaa aaaacttctt caacaaacag atagctttaa
    27421 aacttcaatc tttagataat cgaaatctct catctgcttt gcggatgagg gttttcttat
    27481 tttatgctat acttaaagta tgcatagaaa aacagtgatt gattttagga ctttggggga
    27541 gagatatacc tttacccagc ctattaaaga gttgaaaacg agaaatgtag aagaagtggc
    27601 agatttgctg gcacaagtgg aaaactatca aacgcagggc tactatgtgg tggggtatgt
    27661 cagctacgag gctgcacctg cttttgagga gaaattagca gttcacaagg ttcctctact
    27721 gggcgagtac ttgctttact ttactgttca cgatagggtg gagacctccc ctattcctct
    27781 gacttatgag gatattgatt tgccctcaaa ttggcaggaa gtaacgtctg cagaggacta
    27841 tgaaaaggcc attgcccaga tacaccatca cttgaggcag ggagacacct atcaggtcaa
    27901 ctacaccgtc caactcaagc aaaagttaag tgccaatcct tttgccatct acaatcgtat
    27961 ggtggtagag caggaggcgg gctacaatgc ctatgtggaa catgacgaga tggcagtgat
    28021 ttccatgagc ccagagctct tttttgagca aaatgatcgc gagttgacaa cacgaccaat
    28081 gaaggggacg actcagcgtg gggtaactga ccaagaagat cttgaacagg ccagttggtt
    28141 ggaacaggat cccaaaaatc gctctgaaaa tatgatgatt gtggacctct tgcgcaatga
    28201 tatgaaccgt atttctgaag ttgggagcga gcacgtggag cgtctgtgtc aggtagagca
    28261 gtattcaact gtttggcaga tgacttcgac catcaagagt cagttgcgag aggatgtgga
    28321 ccttgttgaa atcttccgct cactctttcc ttgtggttcc ataacgggtg caccgaaaat
    28381 tgcgacaatg gagattatca aggacttgga gcctcaaccg cgtggagtct actgtggaac
    28441 gattggtctc ttgcttccaa atggacgacg gatttttaat gtggccattc gtaccattca
    28501 acttcacaaa ggtcaagcca tttatggagt tggcggaggg attacttggg atagcacatg
    28561 ggaatctgaa taccgagagg ttcatcaaaa ggcagcagtt ctttatcgta aacaagctcg
    28621 cttccaactg attacgacag gggaaatcag ccagaagaac ctgctgtttg aagatcaaca
    28681 tctggaaaga ctgagaaaag ctagtcgtta ttttgctttt ccttttgatg cagaagactt
    28741 gggacacaag attgaggaag agtgtcagga ttgtgaagct aatcgagatt accgcttgcg
    28801 aatcagcctt agcaaatctg gagagataga agtcaatcgt caagtattaa cccctctcag
    28861 tacaagcttt tgtcaggccc aagtctgtct tcaggaagct gctttgaatc aatcctttac
    28921 ctactttaaa accactcacc gatcgcattt gagcctagga gaacaagaga agatttacca
    28981 caataagtca ggagaactgc ttgaaacctc tataggaaat ttggttctga aaatcgctgg
    29041 aaaactctac acaccgccta tccgacttgg aatcttgcca ggaatttacc gtcagcattt
    29101 gctagaaaca ggacaggtag aagagaaagt cttgaccttg gcagatttaa cccaagcaga
    29161 agctatttac ggctgtaatg cagtgagagg cttgtatgaa ctaagccttg aggagaacta
    29221 gatgaaattg atatttttgc atgggttagg gcagtcagca gagtcttgga aagaagttcg
    29281 gaatctactg acagattatc cttctgaggc tatcgagtta tttccttctg gagttagtaa
    29341 ctatcaacaa gctaaagagc gagtttatca gcacctagct caagagacag aaccttttgt
    29401 tttgattgga ttgtccttag gtgctgccct gacactagaa ctttccagtt acgatttacc
    29461 aaatcttcga gctttgattc tgtcaggatg cccgcttaaa ttagcaggga atatcctatt
    29521 ttatcttcag ttgctgattt ttaaactact tcccaaaaga gtatttgaaa aacagggagc
    29581 agacaagact cttatggtcg gagtttctga ggaattgaag acacttgatt taacggatat
    29641 agcagggact tgctcctatc caaccttact aatttgtggc agcaaagata aaccgaatct
    29701 tagttctatg aaagctctcc atagactgtt aacagattcg cagtttcaga tcattcctga
    29761 tggccctcat gtcttgaata aggcaaaacc aaaggagttt gtagaaaaga ccagaagttt
    29821 ccttgaattg ctgaaataag tttgataaaa taatattgaa atcgatatca taattatggg
    29881 agaaagaaat gaataaacgt ctatttttaa aaatgagtct ggtgacgttg ccaattttag
    29941 ccttgttttc acaaccagtt ttggcggaag aaaacatcca tttttcgagc tgtaaggaag
    30001 cttgggcgaa tggctattcg gatattcacg agggagaacc tggttattct gccaagttag
    30061 accgtgatca tgatggtgtg gcttgcgaat tgaaaaatgc tcctaagggt gcttttaaag
    30121 caaaacagtc aacggctatt caaatcaaca caagttcagc aacaacaagt ggttgggtta
    30181 agcaggacgg cgcttggtac tactttgatg gaaatggaaa tctagtgaaa aatgcatggc
    30241 agggaagcta ttacctgaaa gctgatggta aaatggcaca gagtgaatgg atttatgact
    30301 cttcttatca agcttggtat tatttgaaat cagatggttc ttatgcaaaa aatgcatggc
    30361 aaggagctta ttaccttaaa tcaaacggta aaatggcaca aggtgagtgg gtttatgatt
    30421 cttcttacca agcatggtat tacttgaaat cagatggttc atatgctcgc aatgcatggc
    30481 aaggaaacta ctatttgaaa tcagatggta aaatggctaa aggtgaatgg gtttatgatg
    30541 ccacctatca agcttggtat tatttgacat cagatggttc ttatgcttac agtacatggc
    30601 aaggaaatta ctatctaaaa tcggatggta aaatggctgt caatgaatgg gttgatggtg
    30661 gacgttatta tgttggcgct gacggagttt ggaaggaagg tcaagcaagt acagtttctt
    30721 ctagtaatga tagcaatagt gaatattctg ctgctttagg aaaggcaaaa agttataatt
    30781 cgttattcca catgtcaaaa aaacgtatgt atagacaatt aacttctgat tttgataaat
    30841 tttcaaatga tgcagctcaa tatgccattg atcatttaga tgattaacag atactggtag
    30901 gcgaaaaaat tccttataat tcatttacgc aaacctttgc ataagtgaat ggattttgat
    30961 atactagtac tgtaagcatt ttcaattact acacaatgaa aaaggagaat atgatgagtc
    31021 aaaagattat tgggattgac cttggtggaa cttctatcaa atttgcaatc ttaacaacag
    31081 caggagaaat ccaaggaaaa tggtcaatca agaccaacat tttggatgag ggaagtcata
    31141 tcgttgatga tatgattgag tctattcaac atcgtttgga cttgcttgga ttggcagcag
    31201 cggacttcca aggcattgga atgggatcac caggtgttgt tgaccgtgaa aaagggactg
    31261 ttatcggtgc ctacaacttg aactggaaaa cccttcaacc aatcaaacaa aagattgaaa
    31321 aagctttggg cattccattt ttcatcgata atgatgccaa cgtagcagct cttggtgagc
    31381 gctggatggg tgctggagat aaccaaccag acgttgtctt tatgacactc ggtactggtg
    31441 ttggtggcgg tatcgtcgca gaaggcaaat tgcttcacgg tgttgctggt gcagcaggtg
    31501 agcttggtca catcactgtt gactttgacc agccaatctc atgtacttgt ggtaaaaaag
    31561 gctgccttga gacagttgct tcagcaacag ggattgtcaa cttgactcgt cgctatgccg
    31621 atgaatacga aggcgatgca gccttgaaac gcttgattga taacggagaa gaagtaactg
    31681 ctaagactgt ctttgatctc gcaaaagaag gagacgacct tgctttgatt gtttaccgta
    31741 acttctcacg ttacttggga atcgcttgtg ctaacattgg ttcaatccta aacccatcaa
    31801 caatcgtcat cggtggtggt gtgtcagctg cgggagaatt ccttctacaa ggtgttcaaa
    31861 aagtttacga tgaaaatagt ttcccacaag tacgcacatc tactaaatta gctcttgcaa
    31921 ctctaggaaa tgacgctgga gttatcggag cagcatcact tgtattgcaa taagataatg
    31981 agagaggaaa atgctaacct agagttagta atgttcctct tttatttttt atgctatact
    32041 agttaggaca atgaatgagg tgaaacgaaa tgacaaaagc agatacgatt tttaaagaga
    32101 atattgaatg cattctaaag gaaggtgttt tctcagagca ggctcgtcct aagtacaagg
    32161 atgggactgt tgctaattcc aagtatgtaa ctggtgcctt ttccgagtac gacttatcta
    32221 aaggagaatt tcctatcaca accttgcgtc ccattgcaat caaatccgcc atcaaagaag
    32281 ttctctggat ttaccaagac cagtcaaata gcttagaagt gcttaatgac aagtacaatg
    32341 ttcactactg gaatgactgg gaagttggag acacgggaac cattggtgag cgctatggtg
    32401 ccgttgttaa gaaacacgac attatcaata agcttctcaa acagttggaa accaatcctt
    32461 ggaaccgccg caatattatt tcgctctggg attaccaagc tttcgaagaa acagatgggc
    32521 tgctcccgtg cgcctttcag accatgtttg atgtccggcg tgttgatggg gaaatctatc
    32581 tggatgcgac cttgacccag cgctccaatg atatgctggt ggcccaccac atcaacgcta
    32641 tgcagtatgt ggctttacag atgatgattg ccaaacattt tggttggaag gttgggaagt
    32701 tcttctattt catcaacaac ctccatatct atgataatca atttgaacaa gctcaggaat
    32761 tgctccgtcg ggagccgtca aactgccaac cacgcttggt tttaaatgtt cctgatggga
    32821 ctaatttctt tgatatcaaa gcagaagatt ttgagttggt ggattatgac cctgttaagc
    32881 cacagttgaa gtttgaccta gctatttaaa agaatagaaa aaagaagttg agaataatcc
    32941 caacttcttt tgtttcttaa cgtgatacgc ggcgacgagc tgctttttta cgattttctt
    33001 cgatgaaagc tgctttttgc tcttctggtt cgattacttt ctttttaaat gcgtatactg
    33061 cacctgcaac ggcagcgaca gttcctgcga cacctgttac aagaccttta gcgaatcctt
    33121 tagccatgag tcttcctcct ttatattctc aatcagccag cctcctcaag aggtcacatt
    33181 tttctgactg acctttttgt gttataataa tagtaacgaa aaaatgggaa tttttcaagg
    33241 aaaaaagatg aaaacaaaaa taattgtgat tgttggaccg actgctgttg gaaagacggc
    33301 tctagctatt gaagttgcaa agcgctttaa tggcgaagtg gttagtggag atagccagca
    33361 agtctatcga ggacttgata ttgggacggc caaggctagt ccagaagagc aggcagctgt
    33421 tcctcatcat ttaatcgatg ttagagagat aaccgagtct tactcggctt ttgattttgt
    33481 ttcagaagct aagatgacta ttgaggatat tcagagccgt ggcaagctag ccattatcgc
    33541 cggtgggact ggactttata tccagagctt gctagaaggt taccacctag gtggggagac
    33601 tcctcatgag gagattttag cttatcgagc tagtttggag ccatattcag atgaggaatt
    33661 agcccatttg gtggagcaag caggccttga gattccccag tttaatcgtc gtcgtgctat
    33721 gcgtgcctta gaaattgccc attttggtca ggatttggaa aatcaagaga ccttgtatga
    33781 accgctgatt atctgcttgg atgatgaacg tagtcaactt tatgagcgta tcaaccaccg
    33841 agtggatttg atgtttgagg ctgggctttt ggatgaggcc aagtggctgt ttgaccattc
    33901 ccctaatgta caggctgcta aaggaattgg ctataaggaa ctctttcctt atttccgtgg
    33961 agagcagacc tttgaggaag ctagtgagag tcttaaacag gcgacccgtc gttttgccaa
    34021 gcgtcagctg acctggttcc gtaatcgcat gcaggtcacc ttttatcaga ttggagaatc
    34081 tggtgtacaa gaccgcattt taagccagat agaggagttt ttagatgatt gaaacggaga
    34141 aaaaagagga gcgagtcctg ctgattggtg tggaattgca gggtatggac agttttgacc
    34201 tctccatgga agaattggct agtttagcga aaacggcagg ggcagtcgtt gtagatagct
    34261 acagacaaaa acgtgaaaaa tatgattcca agaccttcgt cggctctggt aagttggaag
    34321 agattgcgct tatggtggat gcagaagaaa tcactactgt catcgtcaac aatcgtctga
    34381 ccccaaggca gaatgtcaat ctagaggaag ttctcggtgt taaggtcatt gaccgtatgc
    34441 agttgatttt ggatatcttt gccatgcggg ctcgaagcca tgaagggaag ctccaagtcc
    34501 acctagccca actcaaatac cttttgcctc gcttggttgg tcaggggatt atgctcagcc
    34561 gtcaggcagg gggaattggt tcccgtggtc ctggtgaaag ccagctggag ctgaaccgtc
    34621 gtagcgttcg caatcaaatc acggatatcg agcgccagct caaggtggtt gagaaaaatc
    34681 gggcgactgt cagagaaaaa cgtttggagt ctagcacctt taagattggt ttgattggtt
    34741 ataccaatgc tgggaaatca actatcatga acatcttgac cagtaagacc cagtatgaag
    34801 cagatgagct ctttgcgact ctggatgcga caaccaagag tattcatctg ggaggcaatc
    34861 tccaagtaac tttgacagat accgttggct ttattcaaga tttgccgaca gagttggtgt
    34921 ccagtttcaa gtcaaccttg gaagaaagca agcatgtgga ccttctggtt catgttatcg
    34981 atgctagcaa tccttaccac gaggagcatg aaaaaacggt tctctccatc atgaaagacc
    35041 tggacatgga agatattcct cacttgacgc tttataataa agcggatttg gtggaggatt
    35101 tcacgcctac ccaaacgcca tataccctca tttctgccaa gtctgaggac agtcgtgaaa
    35161 acttgcaagc attattgcta gataagatta aggaaatttt tgaagcattt accctgcgag
    35221 tgcctttttc aaagtcctac aagattcatg atttagagag tgttgcaatt ctggaagaac
    35281 gtgattatca ggaagacggc gaagtgatta caggctacat ttccgagaaa aataaatgga
    35341 ggttagaaga attttatgac tgatattaaa actttggctc taaagtatgg aggttataca
    35401 agtctggaca aggtctatct ggatcagctt ctagctggta aaacagagca ggagcagtta
    35461 acacttatta cacctccgcc aagtgttgtt aatgcctact ttgcagaact ctatcagaaa
    35521 aaaagtcctc aggctgcgac ggattatttt gcagaactca gtcaggaact gaatctttac
    35581 aatactgagc caagtttcac cctagaaaat aagcctttta ttcggcttaa tctgtctggt
    35641 aaatcctttg gtttttgtta tgagagtgac ggctttggtc ggattttctc tgaaaatgag
    35701 gagaaaatct cggatgactt gctctttgaa attgcgcaaa ttttctccca tcaattagtc
    35761 tttgaagagt ctggtaagat ttacatgaag cctgtcaagg acgaggaagt tgtcagccta
    35821 gaaagtctca cagctttgac tgatttggaa agattggctg atggtcgaaa acgtctcaaa
    35881 ggctacagcc aagaggattt attgcaagaa gctgctgctt tttctggcaa acgctatttc
    35941 cgatcggaaa accgcacagc catgttatat attgattaat tagaaagtat cgaatggata
    36001 ttcaattttt aggaacgggg gctggtcagc cctctaaagc ccgcaacgtt tcaagtctcg
    36061 ccctgaaact tttggacgag attaacgaag tttggctctt tgactgtgga gaaggtacgc
    36121 aaaatcgcat tctggaaacc acaattcgac cacgtaaggt cagcaaaatc tttattaccc
    36181 atctgcatgg agaccacatt tttggtttgc caggtttcct ttctagccgt gcctttcagg
    36241 ccaatgaaga gcagacagat ttggaaatct acggacctca aggaatcaag tcatttgtct
    36301 taaccagcct tcgtgtgtca ggttctcgtc tgccctaccg cattcatttc catgagtttg
    36361 accaagattc tctgggtaaa attcttgaaa ccgataaatt cactgtgtat gcagaggagc
    36421 tggaccacac tattttctgt gttggctatc gtgtcatgca aaaggatcta gaagggacgc
    36481 tggatgctga aaaactcaag gctgctggtg ttccgttcgg cccgcttttt ggtaaaatca
    36541 aaaacggcca ggatcttgtt ttggaagacg gaactgaaat caaggcagca gactatatct
    36601 cagcgccacg tccaggtaag attatcacta ttttaggaga cactcgaaaa acggatgcca
    36661 gtgtgcgtct ggctgtcaat gcagatgtcc tagttcatga gtccacttat ggcaagggtg
    36721 atgaaaaaat tgctcgtaac catggtcact caactaatat gcaagctgca caagtagcgg
    36781 tagaagcagg tgccaaacgc ctcctactca accatatcag tgcccgtttc ctctcaaaag
    36841 atataagcaa actcaagaag gacgctgcca caatttttga aaatgtccat gtggtcaaag
    36901 acttggaaga agtggaaatc tagcagtcac agaaaggata agtatgccta ctattctcat
    36961 taccggagct agcggtggtc tagctcaaga aatggtcaaa ctcctgccca atgaccaact
    37021 catcttgctt ggtagaaata aggaaaaatt agcccaactc tacggaaatt attcccatgc
    37081 agaattgatt gaaattgata ttaccgacga ttcagcccta gaagctctgg taactgatct
    37141 ttatctccgc tatggcaaga ttgatgtctt gattaacaac gctggttacg ggatttttga
    37201 gggatttgac cagattgctg ataaagatat tcaccagatg tttgaggtca atacctttgc
    37261 cctgatgaat ctgtctcgtc accttgcggc tcgtatgaag gaaagcagca aagggcatat
    37321 catcaacatc gtcagcatgg caggtctaat agctactggc aagtctagtc tttactcagc
    37381 gaccaagttt gcggctattg gtttttcaaa tgctctgcga ctcgaactta tgccctatgg
    37441 agtctatgtg acaacagtca atccaggtcc aatccgaaca ggattttttg accaagctga
    37501 cccagatgga acttatctta aatcggttga ccgcttcctc ttagaggcag atgcagtggc
    37561 taaaaagatt gtcaagatta taggcaaaaa taaacgagaa ctcaatctcc cgattttgtt
    37621 gaacctagcc cataagtttt atactctctt tcccaagcta gctgataagt tggcagggga
    37681 aacttttaat tataagtaaa aagaaccaat gtgcaggttg ttgctagcct acatattggt
    37741 tcttatcatt tctcaactat caaactgaac ttcttctagt agatagtcga taaagcgttc
    37801 gcccatctta gataggctgg ttttctcatg ctggatatag accagctcaa tcgggtcatc
    37861 aatatccagt gggatagaaa cgatattgtc tccgtttagg ttgctgttca aaatccctgt
    37921 cgcaatggta taaccatcca aaccaatcaa gagattaaag agggtggcac ggtcactgac
    37981 cacaatggat ttcttgtggt gttcttgaga aagaatctct tctgaaaagt agaaggagtt
    38041 gtgcgtccct tggtcatagc tgaggtaagg gaaattctcc aaatcagaca gtttcacctt
    38101 gtctttcttt gccagagggt tggtcttgct gacaaagata tgcggttgcg ctgtgaagag
    38161 atggtgggct agcaggtgat tgtcatccag catcttggtt aaaacatcac ggttgtaact
    38221 gtttaagaag aggaccccga cctcactgcg gaagttcttg acgtcgtcga taatctccca
    38281 agtccgagtt tcacgaagga agagttcgta tttctccata tcgcttttct tgagcaaaga
    38341 gacaaaggca ttgaccacaa aggcatagtg ttgagacgaa acgctaaaga gttcgcggtg
    38401 ggcgacagga tttttatagc gttcctccag aagctgggtc tgctcgacaa cctgacgggc
    38461 ataagagaga aactccatcc catcacgggt caaggtgatt cccttgggat tgcggataaa
    38521 gatctcaatg cccatttcat tttccaaatc tctcacagca ttggaaagac ttggctgagt
    38581 gataaagagt tgcttggctg cctcattcat ggagccagtt tcgacgattt tgataatata
    38641 atgtaattgt tgaattctca tgtttttatt gtaccacact tgccttagaa tcgctaaaga
    38701 agataggaaa ataaggagca ttgtgtgagt ctcttcttga aaaaaacaca aaattttgct
    38761 agaatgagtc ttatgaaaac attctatgat gtgcaacaat tcctcaaacg atttggtatt
    38821 attgtttaca tgggaaaacg cttatatgat attgaactga tgaagttgga actatctcgg
    38881 atttacgatg cagggttgat ggataaatta gactatctag aggcagaagc agttcttcgc
    38941 agagagcaca aggtagaatt ggattatatt gagaaaaatg gagaaaagaa ctaatggtta
    39001 cttggatttt gtgggcactt atactagcaa tgttggcgtg gatgggcttt aactatcttc
    39061 gtattcgccg tgcggctaaa attgtggaca atgaggagtt tgaagccttg attcgtacgg
    39121 gtcaattgat tgatttgcgc gacccagcag aattccacag aaaacatatc cttcgtgcac
    39181 gcaatattcc ttcaagtcag ttgaaaacta gtcttgcagc ccttcgtaaa gataaacctg
    39241 tccttctcta cgagaaccaa cgtgcgcaac gagttacaaa tgcagctctt tacttgaaaa
    39301 aacaaggttt ttctgagatt tatatccttt cttatggctt ggattcttgg aaagggaaag
    39361 tgaagactag ctaaccttat ttccaaatta gtcgaatgat aggatgaagc tattgtttta
    39421 tagtataatt aatttttata aaatttaaag agttcttaga acatgaaaga aaagaagaaa
    39481 aatccacttt ttgtgggtat cttgtcgatt attcttggtt tgttatttcc aattgtaggc
    39541 ctgattttgg gtatcatagg attggtcttg gctatttcat accaaaaaga gtcacaatta
    39601 gactataaaa tagaaaagat tcttaatatc ttaggaattg tgatttctgt agttaactgg
    39661 attgtagcaa tcgccttaat ttttcgataa ataaaagaaa aaagctttag ttttaaagct
    39721 ttttttgttt tatcgactca tctctaaata gtttttaatg atttgtaact ctttttggtt
    39781 caaagggcgg tagttacctt ctgctaaatc tgggttcaat gtgaagtccc caaattggat
    39841 tctcttgagg ctagtcacct taacaccaac cgagaggaac attttcttga tttgatgaaa
    39901 ttttccttct gaaatggtga tagaggcttg actgatggag ggacttgcag atagaatctc
    39961 tagttttgcg ggtttacaga cagtaccatc taaaaagaca attccctttt gaaaggcttg
    40021 gatatggtca ggtgttagaa gtccattaac ctcaacttga taagtcttat cgacatgata
    40081 ttggggatgg aggagttgga agcccaaggg accgttatcg gtcaagagaa ggagtcctgt
    40141 tgtatctcgg tccagtcggc cgacggcata gagcttgtca gactggatgt cagatggaag
    40201 caggtccatg acggtcggaa gttccttgtc tttgttggct gtaacgacac cagcaggttt
    40261 atgaagcata agataggtgt gttcatagcc ttgaatgatt cgatcctgaa aaaggagttc
    40321 ttgtagtcct gtatcgatat tttgggctag ggagcgggct gggcaactat cgacgagaat
    40381 gtcccctttg aataaggctt gtttcatggc ctttcggctg attttttctt gagctaataa
    40441 attatctaaa cgcatactgt gcctatctta tcataagtcg cttgctttga aaaggcttga
    40501 cgtgaaaaga aaagcggagt gaaaacattt acacttgctt gagttatgtt atttatttga
    40561 aattatggta taatcgttca gttagaaaat aaattttgaa tattatagag gaaatcatga
    40621 caaaattaag agaagatatc cgtaacattg cgattatcgc ccacgttgac cacggtaaaa
    40681 caaccctcgt tgacgaatta ttgaaacaat cagaaacgct tgatgcacgt actgaattgg
    40741 cagagcgtgc tatggactca aacgatatcg aaaaagagcg tggaatcacc atccttgcta
    40801 aaaatactgc cgttgcttac aacggaactc gtatcaacat tatggacaca ccaggacacg
    40861 cggacttcgg tggagaagtt gagcgtatca tgaaaatggt tgacggtgtt gtcttggtcg
    40921 tagatgccta tgaaggaacc atgccacaaa ctcgtttcgt attgaaaaaa gccttggaac
    40981 aagaccttgt cccaatcgtg gttgttaaca aaatcgataa gccatcagct cgtccagcag
    41041 aagtagtgga tgaagtcttg gaacttttca tcgagcttgg tgcagatgac gaccagcttg
    41101 atttcccagt ggtttatgct tcagcgatca acggaacttc ttcattgtca gatgatccag
    41161 ctgaccaaga agcgactatg gcaccaatct ttgacacgat tatcgaccat atcccagctc
    41221 cagtagataa ctcagatgag cctttgcagt tccaagtgtc acttttggac tacaatgact
    41281 tcgttggacg tatcggtatc ggtcgtgtct tccgtggtac agttaaggtt ggggaccaag
    41341 ttaccctttc taaacttgac ggtacaacta aaaacttccg tgttacaaaa ctcttcggtt
    41401 tctttggttt ggaacgtcgt gaaatccaag aagccaaagc gggtgacttg attgccgttt
    41461 caggtatgga agacatcttt gtcggtgaaa ccatcactcc gatagatgca gtagaagctc
    41521 ttccaatcct acacatcgat gagccaactc ttcaaatgac tttcttggtc aacaactcac
    41581 catttgctgg taaagaaggt aaatgggtaa cttctcgtaa ggtggaagaa cgcttgcagg
    41641 cagaattgca aacagacgtt tcccttcgtg ttgacccaac tgattcacca gataaatgga
    41701 ctgtttcagg acgtggagaa ttgcacttgt caatccttat cgaaacaatg cgtcgtgagg
    41761 gctatgaact tcaagtatct cgtccagaag ttatcgtaaa agaaatcgac ggtgttaaat
    41821 gtgaaccatt tgaacgtgta caaatcgaca ctccagaaga ataccaaggg tctgttatcc
    41881 aaagcctttc tgaacgtaaa ggtgaaatgt tggatatgat ttcaactggt aatggtcaaa
    41941 ctcgtttggt cttccttgtt ccagcgcgtg gtttgattgg atactcaact gagttcttgt
    42001 caatgactcg tggttacggt atcatgaacc ataccttcga ccaatacttg ccattgattc
    42061 caggggaaat tggtggacgt caccgtggtg cccttgtttc tatcgatgct ggtaaggcta
    42121 caacttactc aatcatgtct atcgaagaac gtggtacgat ctttgtcaac ccaggtactg
    42181 aggtttatga aggaatgatc atcggtgaaa actctcgtga aaatgacttg acagttaaca
    42241 tcacgaaggc aaaacaaatg accaacgttc gttcagctac taaggaccaa acagctgtta
    42301 tcaagacgcc tcgtatcttg acacttgaag agtctcttga gttcttgaac gacgatgagt
    42361 acatggaagt aacgcctgag tctatccgtt tgcgtaaaca aatccttaac aaagcagagc
    42421 gtgagaaagc taacaagaag aaaaaatcag ctgaataaga gctagaaaga gagaaagatg
    42481 gtctatttag tcctaggaat tttactgctc ctactctatg tatttgcgac accagaaagc
    42541 attaaaggga ctgtcaatat cgtcgctatg gtatgtattt tagtggcact cttgatttta
    42601 ttggttctat cttttctgaa aatttttcaa ttaccaacag aaatattcct agcaatagcc
    42661 atgttgatcc tagcttactt tagtgttaga gacatcacac tcatgccagt caaaaaaagt
    42721 aaaagaagat aaaaaagaga gtttcggctc tctttttgct aggaatttta caatagtttg
    42781 aattgctttt tagttaacac catctctttt acttgtatag tatgaatggg aaaaacttga
    42841 ataaaattcg ttaatggcat ctattttata tttgcttagt actggttgca t
//
LOCUS       DAVKQL010000010        41008 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVKQL010000010 DAVKQL010000000
VERSION     DAVKQL010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41008
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.010"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(94..369)
                     /locus_tag="VW561_000455"
     CDS             complement(94..369)
                     /locus_tag="VW561_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU9942785.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(475..1314)
                     /locus_tag="VW561_000456"
     CDS             complement(475..1314)
                     /locus_tag="VW561_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9942786.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRIGRVTGLLSSLLNIRAVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1456..2304
                     /locus_tag="VW561_000457"
     CDS             1456..2304
                     /locus_tag="VW561_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU9942787.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSGWLAFFEHIPLHIDLQ
                     DDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2397..3314)
                     /locus_tag="VW561_000458"
     CDS             complement(2397..3314)
                     /locus_tag="VW561_000458"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEU9942788.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVEQLKADEEVTRNWS"
     gene            3752..3957
                     /locus_tag="VW561_000459"
                     /pseudo
     CDS             3752..3957
                     /locus_tag="VW561_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(4268..4561)
                     /gene="rpmA"
                     /locus_tag="VW561_000460"
     CDS             complement(4268..4561)
                     /gene="rpmA"
                     /locus_tag="VW561_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEU9942789.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(4578..4922)
                     /locus_tag="VW561_000461"
     CDS             complement(4578..4922)
                     /locus_tag="VW561_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEU9942790.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENS
                     SEFVQTRVITEN"
     gene            complement(4938..5252)
                     /gene="rplU"
                     /locus_tag="VW561_000462"
     CDS             complement(4938..5252)
                     /gene="rplU"
                     /locus_tag="VW561_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEU9942791.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(5288..5375)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(5434..6137)
                     /locus_tag="VW561_000463"
                     /pseudo
     CDS             complement(5434..6137)
                     /locus_tag="VW561_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
     gene            6539..6838
                     /locus_tag="VW561_000464"
     CDS             6539..6838
                     /locus_tag="VW561_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEU9942792.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
     gene            complement(7180..8154)
                     /gene="pta"
                     /locus_tag="VW561_000465"
     CDS             complement(7180..8154)
                     /gene="pta"
                     /locus_tag="VW561_000465"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEU9942793.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(8198..9094)
                     /locus_tag="VW561_000466"
     CDS             complement(8198..9094)
                     /locus_tag="VW561_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9942794.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIYIPAEEGFETLEAIEHPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGNGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            complement(9091..9909)
                     /locus_tag="VW561_000467"
     CDS             complement(9091..9909)
                     /locus_tag="VW561_000467"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEU9942795.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIECIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            complement(9893..10564)
                     /locus_tag="VW561_000468"
     CDS             complement(9893..10564)
                     /locus_tag="VW561_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEU9942796.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            10692..11261
                     /locus_tag="VW561_000469"
     CDS             10692..11261
                     /locus_tag="VW561_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEU9942797.1"
                     /translation="MKHLEIELKILLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            11374..12333
                     /locus_tag="VW561_000470"
     CDS             11374..12333
                     /locus_tag="VW561_000470"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU9942798.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            12343..13458
                     /locus_tag="VW561_000471"
     CDS             12343..13458
                     /locus_tag="VW561_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9942799.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLKAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            13463..13810
                     /locus_tag="VW561_000472"
     CDS             13463..13810
                     /locus_tag="VW561_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEU9942800.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            13818..14033
                     /locus_tag="VW561_000473"
     CDS             13818..14033
                     /locus_tag="VW561_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU9942801.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGKKVYQGLVGDLYRFLSHADFLHQN"
     gene            14116..14757
                     /locus_tag="VW561_000474"
     CDS             14116..14757
                     /locus_tag="VW561_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEU9942802.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            14775..15464
                     /locus_tag="VW561_000475"
     CDS             14775..15464
                     /locus_tag="VW561_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU9942803.1"
                     /translation="MKKPVIGITGNEKTHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(15461..16141)
                     /gene="radC"
                     /locus_tag="VW561_000476"
     CDS             complement(15461..16141)
                     /gene="radC"
                     /locus_tag="VW561_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEU9942804.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(16184..18475)
                     /gene="pcrA"
                     /locus_tag="VW561_000477"
     CDS             complement(16184..18475)
                     /gene="pcrA"
                     /locus_tag="VW561_000477"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEU9942805.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGPGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KTAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDSDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKPASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            complement(18666..19468)
                     /locus_tag="VW561_000478"
                     /pseudo
     CDS             complement(18666..19468)
                     /locus_tag="VW561_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(19849..20709)
                     /locus_tag="VW561_000479"
     CDS             complement(19849..20709)
                     /locus_tag="VW561_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEU9942806.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            complement(20725..22002)
                     /locus_tag="VW561_000480"
     CDS             complement(20725..22002)
                     /locus_tag="VW561_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9942807.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            complement(21995..22546)
                     /locus_tag="VW561_000481"
     CDS             complement(21995..22546)
                     /locus_tag="VW561_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9942808.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENKASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            complement(22562..23821)
                     /locus_tag="VW561_000482"
     CDS             complement(22562..23821)
                     /locus_tag="VW561_000482"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU9942809.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            complement(24964..25122)
                     /locus_tag="VW561_000483"
     CDS             complement(24964..25122)
                     /locus_tag="VW561_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942810.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            complement(25132..26442)
                     /gene="obgE"
                     /locus_tag="VW561_000484"
     CDS             complement(25132..26442)
                     /gene="obgE"
                     /locus_tag="VW561_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEU9942811.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLAENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            complement(26506..26640)
                     /locus_tag="VW561_000485"
     CDS             complement(26506..26640)
                     /locus_tag="VW561_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEU9942812.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            complement(26699..26875)
                     /locus_tag="VW561_000486"
     CDS             complement(26699..26875)
                     /locus_tag="VW561_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU9942813.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            complement(26906..28231)
                     /locus_tag="VW561_000487"
     CDS             complement(26906..28231)
                     /locus_tag="VW561_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU9942814.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGILIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGALYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFRTVLYLPQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            complement(28247..29290)
                     /gene="cpoA"
                     /locus_tag="VW561_000488"
     CDS             complement(28247..29290)
                     /gene="cpoA"
                     /locus_tag="VW561_000488"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEU9942815.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAVLKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            complement(29405..29734)
                     /locus_tag="VW561_000489"
     CDS             complement(29405..29734)
                     /locus_tag="VW561_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU9942816.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            complement(29748..30857)
                     /gene="rpoD"
                     /locus_tag="VW561_000490"
     CDS             complement(29748..30857)
                     /gene="rpoD"
                     /locus_tag="VW561_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEU9942817.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            complement(30860..32620)
                     /gene="dnaG"
                     /locus_tag="VW561_000491"
     CDS             complement(30860..32620)
                     /gene="dnaG"
                     /locus_tag="VW561_000491"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU9942818.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVTFMEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKTSEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAAILKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYAQIEQIVNESRQVQRQNRMEGISRPTPITMPIT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            complement(32710..33468)
                     /locus_tag="VW561_000492"
     CDS             complement(32710..33468)
                     /locus_tag="VW561_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9942819.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEELTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            complement(33465..34331)
                     /locus_tag="VW561_000493"
     CDS             complement(33465..34331)
                     /locus_tag="VW561_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9942820.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            complement(34400..35404)
                     /locus_tag="VW561_000494"
     CDS             complement(34400..35404)
                     /locus_tag="VW561_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9942821.1"
                     /translation="MKNKRLIGIISALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     IELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     misc_binding    complement(35517..35734)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(35817..38513)
                     /gene="ppc"
                     /locus_tag="VW561_000495"
     CDS             complement(35817..38513)
                     /gene="ppc"
                     /locus_tag="VW561_000495"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEU9942822.1"
                     /translation="MSLQKLENSSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDVYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYEIILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     gene            complement(38533..39762)
                     /locus_tag="VW561_000496"
     CDS             complement(38533..39762)
                     /locus_tag="VW561_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU9942823.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNRLLK"
     gene            complement(39918..40220)
                     /locus_tag="VW561_000497"
                     /pseudo
     CDS             complement(39918..40220)
                     /locus_tag="VW561_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(40666..40887)
                     /locus_tag="VW561_000498"
     CDS             complement(40666..40887)
                     /locus_tag="VW561_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9942824.1"
                     /translation="MYRRLRDLREDHDLTQKQIAKILSFTNSAYAKIERGEHTLTADV
                     LVTLSNFYDVSTDYLLELTDFPDKIRFRK"
BASE COUNT        12355 a         8932 c         7245 g        12476 t
ORIGIN      
        1 aactttttaa ctctatttat tactagattt cataattaaa aagcctactg accaagctag
       61 aaagcttgat acaataggct ttttaaagac tgattattta acagcgtctt taagagcttt
      121 accagctttg aatgctggta ctttagaagc tgcaattgtc atttctttac cagtttgtgg
      181 gttgcgacct ttacgttctg cgcgctcacg aacttcaaag ttaccaaaac cgatcaattg
      241 aactttttca ccagctgcaa gatagtcagc tactgctgca aatacagctt caactgctgc
      301 tgctgagtct ttcttagtca attctgtagc ttctgctact ttagcgatca aatcttgttt
      361 gtttgccatg ttaatgattc ctccaaataa tttctaatta acaaatataa tcatatccta
      421 aaatcccttt taggtcaagt taaaaacgct atttcttccc attttattta ttttttagga
      481 gtggtaacgt atcaaaatag cccaagcgtt ctcacccgtg tgagtttgaa taatggaacc
      541 cgtttccaaa acagaaattg gcttttcaac ataagcttgt aagctttctt tcatctcttt
      601 tgcccaatca tcactaccag aatatgaaat tccaatctct gctacagcac gttcagaaag
      661 cgatgttatc aactcatcta accatttttt aaatgtttta gttccacgac ctttaaccat
      721 tggctgcaat tcatggtctt tcatttgcat gacagcacgg atattgagaa gagagctcaa
      781 caagccagtt acacggccaa ttcgtccacc tttgacaaga ttttccaaag ttgaaacacc
      841 aatataaagc tctgtatggt ttttaacctc ttctacatga gataaaattg cctccatatc
      901 tttaccttct tgagctaact tcgcagcctc aacaacttgg aatttcaggg cttggtcagt
      961 gaaggaacta tcaacaacag tcacatctgc agtagatagg ctagcacctt ggcgtgctgc
     1021 ttctaccgta cccgaaagag catgggacat atgaatagca agaatctggc caccatcttt
     1081 gcataggtct tcaaaaatct cagcaaagac acctacaggt ggctgacttg ttttcggaag
     1141 attcttactt tcttgcatca actgaagaaa tttaccttct tctttcaaat ccgcatcaga
     1201 ataaacaaca ttatcaatca ttacagataa tggaacaatt gtaatatcta attgctttac
     1261 tagttcaggt tcaatagtaa cagatgaatc ggttacaatc ttaatttttg tcatagtatc
     1321 aatctttcta ttttaggatt cagattggtt tccttacttc taattatatc aaaaaaaaga
     1381 ttaaaaatcc taatggagtc aatcaaattt tccgtaaaat ttgatataat caacttataa
     1441 gaaaagaggt gtcctatgat taaaaaaatt taccccattt ttaccatttt actaggtgct
     1501 gctatttatg cttttggact gacttatttt gtagttcccc atcatctctt tgaaggaggg
     1561 gcgacaggca ttaccctcat caccttttat ctttttaaaa tccctgtttc cctcatgaac
     1621 ctgctgatta atattcccct tttcatccta gcttggaaga tttttggagc caaatccctc
     1681 tattctagtt tactaggaac cttagctttg tccggctggt tagctttttt tgagcatatt
     1741 ccccttcata ttgatcttca agatgattta ctaatcacag cccttatagc gggaatccta
     1801 ttgggaattg gccttggaat tatttttaat gctggaggta caactggcgg aactgatatt
     1861 ctagctcgta ttctcaacaa atacactcat atatccatag gaaaactgct ctttatctta
     1921 gatttttgta ttctcatgtt gattctccta atcttcaagg atttgagatt ggtttcctac
     1981 acgcttttgt ttgattttat tgtttctcgt gttattgatt tgattggtga aggaggatat
     2041 gccggcaaag gctttatgat tatcacaaaa cgtcctgacc aacttgctaa ggcgattaat
     2101 gatgacctcg gaagaggtgt tacttttatt tctggtcaag gctactatag taaagaaaat
     2161 ttgaaaatca tttactgtat tgtcggaaga aatgaaattg tgaaaacgaa ggaaatgatt
     2221 catcgaatcg atcctcaagc ctttataact attacagaag cccatgaaat cctaggagaa
     2281 ggcttcacct ttgaaaaaga ataaaaagag gtagtgtcgt gacctcaaaa gttagactaa
     2341 atcatctatc ttttgggtta cagacaacct cttttttgtt ttatttactc aagctcttaa
     2401 gaccaattcc gagttacttc ttcatcagcc tttaactgtt ccactaattg gtcaactgag
     2461 tcaaatttgg tcatatcacg aatgcgatca agccaataaa ccatgacggt ttccccataa
     2521 atatcttgat taaaatcaaa aatattgact tcaaaacgtg cttcttctcc atcaaaggtc
     2581 acatttttcc cgacactagc catagcacga tacttctgtc tttgaatctc aacatcaacg
     2641 acataaacgc catctgctgg catataagta cggtctaaaa gcactaaatt cgctgtcgga
     2701 taaccaattg tacgaccacg agcattacca tgaaccacca tacctcttga tggaagcggt
     2761 gcccccaaaa gttttcctgc ttctttcaca tttccatcta aaatagcttg acggatacga
     2821 gttgaactaa tctttccttt ctcatcttct acaggtggaa caatgataac ttctccatca
     2881 aagtaatcct ttaaatcttc tgctgttttt ttgtcagaac caaatgtata atcaaaacct
     2941 gcaacaataa ttttggcatt catagccttg atataagttg caaagaattc ttgtgcagtg
     3001 agactagcga attgactact aaaatcaagg agatataatt cttctacacc ttcgcgcttt
     3061 aattttcttt cacgttcagc agggttcaaa atatgcaaaa acaaatctgg atgataaggc
     3121 tctaaagcga tctttggaga ttcattaaag gtcataacga cgataggtaa taaatccttt
     3181 ctcgcagcct tgttggcaac acgaaataat tcttgatgcc ccttatgtat gccatcaaaa
     3241 tagccgagaa caacgactga atcagatggt gtgccaatat ctttttggtt ttttatagga
     3301 atagtaataa tcataaaata attatatcat agcgatagct atttctggaa cagaaaatct
     3361 gaaatgttgt ttttttcaca tgaagtgtac ctgttttcaa aaagcacttt attctatcgt
     3421 tgcttaacta tgaactttgc aatattcttc tcaaaaactt gtaggacatc ttcaaaattt
     3481 tgcaaggagt gattagactt gttcggtaac cataaagtgt catactatgc ttatgtatga
     3541 aaaagcaatg caactaactc ctgagaactt taaattacta attggtgccg aaaaggtaga
     3601 atttagaatc gaggtacacc tatggctgta aaatttacaa aatgagacaa cttgagcaag
     3661 atgtttgaag aatttcctaa actccctgat ttgaagcaag tcactttccc tgatgacaaa
     3721 gaaaaaagcc aaaacagcaa agaaaaacta gatgactgct ttccaacaac tcccatctag
     3781 tgtgcttcag actaggctat ttttctctcc atctgttagc ttggattctc agaccgtttc
     3841 agctaaagaa tatcttttcc cttatcagaa ggaacggctc aaaccattca gacaagtgaa
     3901 gggacgacaa gccaatattt gaaaccagat agcagttctt atagtcaatt gaaataaaat
     3961 ctgaagaaat cgagtaggaa actcatatca atttttaaca gtgttctatt ccagattcat
     4021 actcaatgaa aattaaagtg caaactagga agttagccgc aggctgtact tgagtacggc
     4081 aaggtgaaac tgacgtggtt tgaatttgat tttcgaagag tatcaatcca ctatacatat
     4141 taaaaagtat ggatatcaaa acaaaaaaac aggctctccg aaaactcgga aagccttatt
     4201 ttaagctgtt tctagcttcc tcacctttac atttcaaggt tcgggataaa aggttcaatg
     4261 gaccttttta tttagcgatt gggtaaacag aaacttgttt tttatcgcgt cctttacgtt
     4321 caaagcgtac tacgccttca actttagcga acaaagtatc gtctccacca cgaccaacgt
     4381 ttacacctgg atagatgtgt gtaccacgtt gacggtaaag gattgatcca cctgttacag
     4441 tttgtccgtc agctgcttta gctccaagac gtttcgcttg tgaatcacgt ccgtttgatg
     4501 tagaacctcc accttttttg tgggcgaaaa gttgcaagtt gttaagagtc atttttaaca
     4561 taatgttttc ctccgtgtta gttttctgtg ataactctgg tttggacgaa ctcagaagag
     4621 ttctccgata agtttgccat acctaagaaa aatgattcaa agaataactg ggtcatttct
     4681 ctctggtgtg aaggaagatc ttttggtatt tcaaccatca gatagccacc ttcatcttcg
     4741 tttaattcta ggattggttc atagcctgca aatttctcaa tagaattgat aaagttaatg
     4801 gcaagcgtag aaaccgatgc acacacgaca tctaagccgt attcgccact ctcggcgtgt
     4861 ccagtaattt ccgcactcct cagctcgcca tcttcggctc tctcaaagac tgcctgtatc
     4921 atgtgttctc cttaaaatta agcgttgatt gcgttgatga caacttttgt atatggttga
     4981 cggtgacctt gtttacggtg gctacctttt ttaggtttgt acttgtaagt aaccactttc
     5041 ttttgttttc cttgtttttc aacagttcca actacagtag ctccagcaac aagtggagtt
     5101 ccgacaacag tgttttcacc accaacaaga acaacttcgt taaaagtaac ttcttgacca
     5161 gcttcaacgt tcaatttttc aacgtaaact gcttgaccaa cttcaacttt aacttgtttt
     5221 ccgccagttt tgataattgc gtatgtgctc attatgcacc tcctatgatt tttatgggtt
     5281 tccccgattt tttgtgaaga ctcgcctagc atcgtgggac gaaccactta aacttgaata
     5341 atcaagcaac gatgttcgtg cggttgcaca ggatcgtgca tagtcaactc ttcaagtata
     5401 gcatatctcc tattttctta caagtaataa cacctaaaat gaagcttttt cttttacttt
     5461 tttctgccaa gaggcaaaaa gcatgctgag gtaaaaaacg ctcatcataa taggaacacc
     5521 aagaatggtc ttttcatgat agaaaatcgt caaataggct gaaaagacaa cgccaaggac
     5581 aaaactacta agcaggctaa caaatatgaa tccttcacgc aaaaaagagt gtgcttggtt
     5641 cggaaataat ctccaaaagc cagcatggtc cgtttgatat tccctgtcat aaaagcgtta
     5701 ttataggcaa tacccgacac ttctccaaaa gcagttgtca ccagtcccat acagaaggcc
     5761 aagggcggca ctagatagat attatccaca gtttgcggca caaaagcaat aatgattgat
     5821 aagattgcca agggaatcaa ggacagaata ggttttttca caattctcaa tttttcctta
     5881 taaatcgtta ataaaaagac tcccatcata aacgctagca aggtgagaac cttgtcccta
     5941 acatccgaaa cattattttt aattaattct actgaaagaa agacaacatt tccagtttgt
     6001 ccagctacaa gggtattccc gcgaacaata aaagtgtaag catccacata tccagcacaa
     6061 aacgtcaaaa aaagtgctaa ccttttagac tgacgtgata tttttcttat aggtaataac
     6121 ctcattttac ctcccattgt attttctctt agaaatattg taccattttc tttctaaaaa
     6181 atcgtaggct accatttaga ttttactatt agcataaaaa taataataga caactattta
     6241 tccaaaaata gatagatgta acatgtttgc aaacaaagca tacgaacctt tagtaaaatc
     6301 atttccatga aactagaata gagccctctt agcaaaaatc attattttaa tttatttcta
     6361 atcactcctt gacataaata actctcacca ataaaagact atgtcttaaa aaaatggtat
     6421 aataaaatca atacttgggc ttgatggcta tgctactaat aacaattagg agagaaaatc
     6481 aggcacttgt taacaacaag gattatcccc ttgagatgaa aggaacttta gaaatcttat
     6541 gatgaacatg caaaacatga tgcgtcaagc acaaaaactt caaaaacaaa tggaacaaag
     6601 ccaagctgaa cttgctgcta tgcaatttgt tggcaaatct gctcaagatc ttgtccaagc
     6661 gaccttaact ggcgataaga aagttgtcag cattgatttc aatccagctg tcgttgaccc
     6721 agaggacctt gagactcttt ctgatatgac cgttcaagcc atcaactctg ctcttgaaca
     6781 aatcgatgaa actaccaaga aaaaactggg tgctttcgct gggaaattgc cattctaaaa
     6841 acaaggagct agaacaatac ttgtcgataa caaaggctaa gaaaggtgca aaaattataa
     6901 aaaacgcata atagcaggtg tcaagacact taatattatg cgttttctca tgaaaaaatt
     6961 ctcttttcat actcatttaa aatcaaaaag ggaatttatc gttttacgga catgtatatt
     7021 acagatttct ataggtcgtg tatagacagg atatcatcgc ttggaagttt tcctttttga
     7081 caccaatcag taatttagtt ctcaggaatt aatggcagtg cttttcgtac atagtttcat
     7141 aactacagta tagcatagta tatcacttta tagttttcac tattgatgaa ctgcttgagc
     7201 tgctgtgatg agggtcaact tgtaaacatc atctgcatta catccacgag aaagatcatt
     7261 aactggcttg tttaaacctt gcaaaacagg tcctacagcc gcaaagccac ccaggcgttc
     7321 agccatcttg taaccgatat ttcctgcctc gatacctggg aagatgaaga catttgcttg
     7381 accagctact gtacttcccg gagctttcag agctgcagtt tcgggaacaa aggccgcatc
     7441 aaattgcaac tcaccatcga tttcaaggtc aggacgcaag tcgtgagcaa ttttagttgc
     7501 ttcaacgacc ttatcaacgc tttcaccaaa ccctgaacct ttagtagaat agctcaacat
     7561 ggcaatttta ggttcgatgc caaacatctt agctgtgatt gctgagttga tggcaatttc
     7621 agccaaggct tctgcatctg gattgatatt aatggcacag tctccaaata ggtaacgttc
     7681 cgtaccacga accatgagga aggctcctga agtacgagtt acatttggac gagttttgat
     7741 gatttgtaga gctgggcgaa ctgttgaagc tgttgagtga atcgctcctg acaccattcc
     7801 atcaaccaag cccaagtaaa ccaacatcac accaaagtag ttgacatctt caaccaaaac
     7861 cttgcgtaca tcttcttcag tcattttgcc cttgcgacgc tccaccaagg cagaaaccat
     7921 ttcttcaaat tgaggataat gttgagggtc gatgacctca taaccatcca tgattccttc
     7981 aatttcaaga taaattttaa ttttttcagg atttccaagc aaaacaggaa tcacttctgt
     8041 ttcttttact aagcgttttg ttgcttgaag aatacgaggc tcttcccctt cagggagaac
     8101 gatacgagca tttttaccaa caaggttggc tttgagactt tcaaaaactt ccatgagttt
     8161 tctcctttaa gataataatt atactctgag acagttttta tagagtatta gttgataact
     8221 gggtaataag gttactgaaa tcatccggca agggactttc taactgcaag tcttgctcta
     8281 aaaatggatg ataaaaggat aggtaatggc aatgcagagc ctgacgttga ataccatctt
     8341 ccagactacc accatacaaa tcatctccca gcaaaggaaa accgatatga gaaaaatgga
     8401 ctcggatttg atgggttcga ccagtgtgca ggtgaatata gaccaagtga atatttccat
     8461 aagaagctac aatcttgtat gaagtatggg catactttcc gcctttagcc actcgtctgg
     8521 taataatgga atcttcatca cgcgcaatcg gagcaataat ttccccttct ggctccaaat
     8581 gtccatttcc cttaaccaaa gcaaagtagc gtttctcgat agatttcttc tgcaactgct
     8641 tgtctaatcg tgcatgggca taaccgtgct tggcaaagag catcaagcca gaagtgtccc
     8701 tatctagtct ggtaacaatg tgaacctgct gattttcata attttgcttg acatagtaac
     8761 ccttgataaa attggcaatg gtattagagt gattgacact aggaatagaa gccactccat
     8821 agggtttatt caagactaga aagtggtcat cctcatagag aatatctaat ggatgctcaa
     8881 tagcctccaa ggtttcaaag ccttcctcag cgggaatgta aatggtaacg tagtctccaa
     8941 cgtccaatag atacgttgca ttttgcggtt gattattgac cagaatagct ccacctcgaa
     9001 acttaatctt ggccagcaat cccttagaaa cctcgtgctt ttttaaaaag gtcttaacct
     9061 tgacatgttc atctgcgata aattcaaacc tcattcatcc acctcaccga taaaggcatc
     9121 cttaacacgg ttccagaaac tggtatggct aggagtcgcg acaaagtgaa tcttatgatg
     9181 gtcgatttga tactcaatac actcaatatt acggaaagaa taaacgctat tgtcaaccga
     9241 aatagtatga taatcgtttc ttgttggaat aagttcaatc ttatccttct taggcacaat
     9301 aatggaagag cccaatgttc gatagacacg attattaagg ctggcaatct ccgttaattg
     9361 caaagcttca atggtagggt gtaaaacagc accgccaaga gacttgttat aggcagtact
     9421 accagtcggt gtcgaaactg ttagcccgtc tccacgaaaa cgttcaaagg gaacaccatt
     9481 tattacaata tctgccacca tggttcgatc agacctgcgg atgctggctt cgttgagtgc
     9541 tctgaaaatc ttaacttcac cattttcgag aaagaccttc acattcagaa cagggtaaga
     9601 aacccttgcc ccagtatcta gctgcaaatt agtcactagc ttgtccaact caaaatcacg
     9661 ataatctgta tagaagccca aatgtccagt atgaagaccg ataaagcgga ccttgtcaag
     9721 ctgattttcg tacttatgaa aggccgacaa gagcatacca tccccgccaa tggaaatgac
     9781 aatatccgga ttggtatcat tgagtataaa ctgatttctc ttcaaacgat ctcgcaattc
     9841 atacaaaacc ctttgactct gcggttttct attggctatc agatcaattc gtttacctgt
     9901 attcttcatc tgtatcgtca ctgtttccta caccgtcatt taattttcta ctcaaaggat
     9961 caaaaagtgc ctgggcttct tggatatcat cacgaatttc acccatttct tcatccaact
    10021 gatgggcgat tctagctgta atttccagtc gcttcttaat ctcatctggg aaatcccctt
    10081 ggtacttgta gttgagagaa tgttctatcg ttgcccagaa attcatggcc aaagtacgaa
    10141 tttgaatttc tgccaaaata gtcttagctc cattgatggt atcaaccgta tattctacta
    10201 ccacatgata ggaacgatag cctgatgctt ttctatgagt aatgtaatct cgctcctgta
    10261 tgattcgcat atcctgacgc ttgtgcaaaa tatccactac ttccttgacg tcatctacaa
    10321 actgaaccat cacacgtaag ccagcaatat cctgcaaatc gtgttccaag gtcgcataag
    10381 taatgccacg acgagccatt ttttctttga tgctctcaat tggcttgact cgaccggtca
    10441 caaactcaat tggagaatgc ttattttgct tacgatattg cttacgaata ccacgaagtt
    10501 taatctttaa ctcaccaaca gcttgaatgt aaggatctag aaattcttcc cattctaagg
    10561 tcatatattc tcccttgttc tcatattatc cgtcaaaaat atctttgatt ttttctccag
    10621 attcatatac aataaataca atgcttatta taccataaat ccgctttcaa cgataaaaaa
    10681 aggtaagaaa aatgaaacat ttagaaattg aattgaaaat actattgaaa aaagatgaat
    10741 acaatcgtct aaaagaccag ttcacaggtg tcactcctgt tcttcaaaca aattactaca
    10801 tcgacacgcc tgattttgaa ctgcgagaaa agaaagtcgc tatgcgcatt cgaacctttg
    10861 aagactgggc tgagttgaca ctcaaagtcc cgcagagtgt tggaaacatg gaatacaacc
    10921 aaaaattgca actaaaagat gctgagaact atctgagtaa ggaagaactt cctcaagggc
    10981 tagtactgga tgaattggcg aaacatggta tccaaagtaa gaactggcag gttcttggtt
    11041 gtctaacaac gcttcgctat gaaatgaaaa cagctattgg tctcatggca ctggatcaga
    11101 gccgatactt tgatataaca gattacgaat tggagcttga agtggaaaat catgagcaag
    11161 gcaaacagga tttccgacaa tttttagaga aaaatcagat ttcctaccaa aaagctcctt
    11221 caaaattggt tcgatttgtc aaaagtatga aaaatagctg aaataatctc cattttttgg
    11281 taaaatagaa aagataaaaa tacacaaatc ccagttcata tatgtaaggt aaatataact
    11341 aggatttata aagtttacag aggacggtct ataatgtcag atagaaaaaa catgaaactt
    11401 ttcgcactca actctaacca agagattgca caaaaaattg cccaagctgt tggtgtccca
    11461 cttggaaaac tatcatcacg tcaattttca gacggagaaa tccaagtaaa tatcgaagaa
    11521 agtgtccgtg gttatgatgt ttacatcatc caatcaacaa gtttccctgt caacaaccac
    11581 ctaatggaat tgttaatcat ggtcgatgct tgtgtgcgtg caagtgccca cagtatcaac
    11641 gttgtccttc catattttgg ctatgcacgt caagaccgca ttgcttgtcc tcgtgagcca
    11701 cttacagcaa aactagttgc caatatgctg gttaaggctg gagttgatcg tatcctgact
    11761 cttgatttgc atgccgttca ggttcaaggt ttctttgata ttccagtgga taatcttttc
    11821 actgttcccc tattcgcaaa acattactgc gataaaggat tgcttggttc agatgttgtt
    11881 gtcgttagcc ctaaaaattc aggtgtcaaa cgtgcgcgta gcctggctga atatcttgat
    11941 gctcctatcg ccattatcga ctaccctcaa gacgatgcaa ctcgtaacga aggttatatt
    12001 attggtgatg ttgaaggtaa gaaagctatc ttgattgatg atattttaaa tacaggacgt
    12061 accttctctg aagcttctaa aatcgttgaa cgtgaaggag ctacagaaat ttatgctgtt
    12121 tctagccacg gtctcttcgt cgagggagct gctgagcttc ttgacaatac taatattaaa
    12181 gaaattcttg tgactgattc agtagcaaca aaagaaaaaa ctcctaaaaa tgtatgctac
    12241 atcactgcta gtgagttaat tggtgatgct atcgtccgta ttcacgaaag aaaaccagtc
    12301 agcccactct ttgcctacaa taaaaagaaa taaggtgatt ctttgattta tttggacaat
    12361 gctgcaacga ctcctatgtc agcagttgct atttcagcta tgaccaaggt tatgcaagaa
    12421 acccacggaa atccttctag tattcatggt catggtcgtc aagctggcaa actcttgcga
    12481 gaagcccgtc aggaactagc ccagttacta aggacaaaac ctcaacatat ctttttcact
    12541 tctggtggga ctgaaggcaa taatactacc atcattggct actgtcttcg tcaccaagaa
    12601 caaggaaaac atatcatcac aactgccatc gagcaccatg ctgtccttga aacaattgat
    12661 tacttggttc aacactttgg gtttgaagca accattatcc agccagaaaa tcaagaaatc
    12721 acagcccagc aaattcaaaa ggctttacgt gacgatacga ttttggtttc taccatgttt
    12781 gccaataatg agacaggaaa cctactgccc atcgctgaaa ttggccaaat actcaagcaa
    12841 caccctgctg cctatcatgt tgatgcagtt caggctattg gtaaaatccc aattcattca
    12901 gaagaattgg gcattgattt tctcactgct tctgcccaca aattccatgg tcctaaggga
    12961 atcggttttc tctacgcatc tagcatggac tttgattcct atctacatgg cggagaccag
    13021 gaacagaaaa aacgtgcagg aactgaaaat ctgcctgcta tcgtaggcat ggttgcagcc
    13081 ctaaaagaag acctagaaaa acaagaagaa cattttcaac atgtacaaaa tctagaaact
    13141 gcctttctgg cagagctgga gggtattcag tattacctga atagaggaaa acatcatctc
    13201 ccttatgttc tcaatattgg atttcctggt cagaaaaatg acctcttact ccttcggcta
    13261 gatttagctg gaatttcaat ctctactggc tcagcctgta ctgcaggcgt tgtccaatcc
    13321 agccatgttc ttaaagccat gtatggcgca aattcagaac gcttgaagga atcccttcgc
    13381 atcagtttgt cgccacaaaa taccgttgaa gacctacaaa ccctcgcaaa aaccttaaaa
    13441 gaaattatcg gaggttagcc atatggcatt tgaaaaaatc attcagttaa aaaattgtcg
    13501 ttacgattac actcttagcc cttctgttaa aaaattcacc ctcaaagata acaccttttt
    13561 tgaaactaag gttggtaact atgaactgac tcgccttttg gaaaaagtgc caaacagcgg
    13621 tgaaggcttc caactcaaaa tcatcattaa caaggaactt acaggggcta aaatcaatat
    13681 cactgacaag tttggccttc gtctagttga tattttcaaa tcagaagacc accatattca
    13741 tcaggaaaaa ttctacttcc tcatggatag cttggtagaa cgtggtgtct ttacaaaatc
    13801 ggaaagatag agccaaaatg tttgaactga cctataagga ctgctatcat gtagagcgta
    13861 ctctcaagta tgaggatcat gaggctctca tgttgacttt gtcaggatgt gtaaccttac
    13921 cagatacact ttatgtgact tctctgactt ttaggggcaa aaaagtttat caggggctgg
    13981 tcggagacct ctaccgtttt ctatctcatg cagatttttt acatcaaaac taagattttt
    14041 cttagttttt tcttattttt gttgattttt tcacaatgtt tctataaaat agaagaatag
    14101 aaaggttgtg attttgtgaa agataaacag tttgctattc caaaagctac agcaaaaaga
    14161 ctctctctct actatcgaat ttttaagaga tttcatgcag aaaagattga acgtgccaac
    14221 tctaagcaaa ttgcagaggc tatcggtatt gattcagcga ccgtacgtcg tgatttttcc
    14281 tattttggtg aacttggtcg tcgtggtttt ggctatgatg tcaaaaaact aatgacattt
    14341 tttgccgatt tgctcaatga caactctatt accaatgtca tgctggttgg tattggaaat
    14401 atgggccatg cccttctcca ctaccgcttc cacgaacgta acaagatgaa gattatcatg
    14461 gcctttgacc tagacgacca tcctgaagtc ggtacccaaa ctcctgacgg gattcccatt
    14521 tacgggattt ctcagatcaa ggataaaatc aaggatgctg atgtgaagac tgctatccta
    14581 actgttccca gcgtcaagtc acaagaggtt gctaatctct tggttgatgc tggtgtgaaa
    14641 ggaattctca gtttttcacc agtccatctg catttaccaa aagacgtggt cgttcagtat
    14701 gtcgatttga caagtgaact ccaaaccctc ctctacttca tgcgaaaaga ggattagaaa
    14761 gcgaaaatca ttttatgaaa aaaccagtta ttgggattac aggaaacgaa aaaactcatc
    14821 cagatgatga catcatgatg agctacgcag caaaaggctt tgttgaaggc gttaaagatg
    14881 ctggagggat tcccatcatc ctaccgattg gtgatcaaga aatggcctgc cactatatca
    14941 gtttgattga caagctcatc ttgacaggtg ggcaaaatgt cgatccaaaa ttttatggcg
    15001 aacccaaaac cattgatagt gatgactatc accttcaaag agatatcttc gaactggccc
    15061 tcatcaagga agctattaaa cagaaaaagc ccattttctc tgtctgccgt ggtacccaac
    15121 tctttaacgt tgccatgggt ggaactttgt accaagatat cgaagaccat tggcaggatt
    15181 cttctgttga gtacacaact caacgcttgg taacagaacc agataccgtt ctccaagaaa
    15241 tctatggaga aatctctcat atcaactcct tccaccacca gagcatcaag gacttagcac
    15301 caaatttaaa agttgtggct catgatccta aagatggtat cattgaggct gtcatgagta
    15361 cggatgatgt cgcctttctc ggtgtccaat ggcatccaga acttcttttt gaaaatcgtc
    15421 ccaaagataa aaaactcttt gactatgtcg ttaatgaact ttagattaaa tctgtctttt
    15481 cacgataact aaagtaatta gaatgagaga caatcaaatg gtccaagaga acaatcccca
    15541 tcaattcgca ggcttcttta acaagtttag tgacatgatc atcattttgg ctaggcgcta
    15601 ccgctcctga aggatgattg tggaccaaga taagagaagt cgccatatgc ttgattgcat
    15661 agtgaagaat ctctcgcggt tcagcgatac tacgagttac agacccgata aaaatggtct
    15721 gctgatggat gatttgattt tgagtattga gatagagtgc caccaggtgc tcttgttttt
    15781 tatcccctaa ttcctgctgc atcttcttgg ccaacttttg actgctgaga atactttcca
    15841 tttcaagagt ctcgtgtttg tgaatacgat gccccagttc aatcatagct tgtaattcta
    15901 tggccttaac acgcccaata ccagacaaac tctgcaattc ctgcagggtc atttttttca
    15961 aatccgttag gcttgaaaga ttgttcaaga ctttttgggc aatttcaaaa acgctagctt
    16021 gacgtgttcc tgtcctgagt aaaatagcta gcaactcttg gttactaagc gcttcaactc
    16081 cttccttggc cagcctttct cttggtaata gtgaatcttc ttggaatgaa atactgtaca
    16141 taaaaatcct cctcacttta ttattcgtga gaaggatgga aaattagatt tttttctcaa
    16201 ttggagccac actggctaaa agttttttca aacctacttc tgggaaattg attttcaatt
    16261 cctgcctagc accgctacct gaaacttcca gaacggttcc ctctccccat ttcttgtgga
    16321 gagcaatatc accaatggac caatttgcct cgctagatgc tggttttgcg ccagctgtaa
    16381 attgaccaaa tggaagaccg cttgactgga ttgattttgg ggcagcaccg cgtttacggt
    16441 cttgaagagc ctgagccaaa ctcatacctt gaccaaagga aatactacca ctgctatatg
    16501 atgccttaaa gcttgtattt gcaggacgag ccagaccttg atactcaagc aagtctgaac
    16561 tgatttcgtt aataaaacga gtcggacggt tataattggt acgaccaaaa agcaagcgtg
    16621 agttggcatt ggtcagatag agaattttct ctgcacgcgt gatacctaca taggctagac
    16681 ggcgctcttc ttctaattca tctgaatctt cagtcgcacg actaagtgga aagacatttt
    16741 cttccatccc aatcaaaaag acaactggaa attcgagacc tttggcagca tgcagggtca
    16801 tcaaggtcac ttctgatgtc tcctgactac ctgaatctgt gtcggcaatc aaagccaagt
    16861 catttaagaa acgactcagt ttgtccagac cagtttcctc ttctgtcaca tccgtggtgt
    16921 catcaaagtt cttcgtaaca gaaagaaact cttcgatatt ttcaacccgt gccttgcttt
    16981 ctagagtcgc ttgggcgtta agaatatcga cataacctgt tttttctagg acggactcaa
    17041 ccaactctgt aatgcttaag tggtctagct gctcccgcaa atcaagcatc atattggcaa
    17101 aatcccagat agattgggct gtcttaccct tgataccaga caacataata ttagcagaag
    17161 catccagcat agacatattt tgcaaatttg caaaatcacg gattttctca actgtacctg
    17221 gaccaattcc acgtttaggc tcgttgataa tacgctcaaa actaatattg tcactcaaat
    17281 tagcaataag gttgagataa gcaataatat cgcgaatttc cttacggctg tagaatttgg
    17341 ttccgccaac catggtataa ggaatgttag acttgagcag ggcttcctca attgtacggg
    17401 actgggcatt agtccgatag agaactgcaa aatccttatg aaggaagttt tgactgcgac
    17461 taagttcatc gatggttctg gctacaaata cagcctcatc cagctcatca tcggcacgat
    17521 agtaaacgat ttgctcccca tcagcgtttt gagtccagag atttttagga cggcgatttt
    17581 tattattttt aataacctcg ttggccgctt ggagaatggt tttggttgag cggtaatttt
    17641 cctccaacaa aacaacctta gctttggggt aatccttttc aaagtccaag atattctgca
    17701 tatcagcacc acgccaaccg tagatagact ggtccgcatc cccaaccaca cagatatttt
    17761 taaaacggga agccaagagt ttgaccaatt ggtactgagc gtggttggta tcttggtact
    17821 catcaacgtg gatgtattgg aatttttgct ggtagtaggt caaaacatca ggattttgat
    17881 caaagagacg caaggtcagc ataatcaaat catcaaagtc aacggattca gactgacgaa
    17941 gttctttttg ataggctgta taacactggg ccacaatttg cgtatacata tcgccagctt
    18001 gggcagcata agcaacatca tcaatcaaat cattcttagc attggaaatg gtccccaaaa
    18061 tagttcgttc attccatttt ttaggatcca agttcaactg tttgagaata cgtttcatga
    18121 gcgttcgctg ttcaccagga tccacaattg taaaattacg attgtagcca atatggtccg
    18181 catcgcgacg caaaatacgc acacacatgg agtggaaggt cgcaatcaga cagtcctgag
    18241 tcgctggatt gaggctataa gcacgctctt tcatctcacg cgcagccttg ttggtaaagg
    18301 taatggccaa gatattccaa ggattgacca gcttttcatc aatcaaataa gcgatacggt
    18361 gggtcaaaac acgagtcttt ccagaaccag cccctgccat gattagcaag ggaccttctg
    18421 tcgtttgcac cgcctcagcc tgacggtcat tcattccatt taataatgcg ttcatcttct
    18481 cttccttact cttgaagtca atatttctat tatatcagaa atcagggaaa atagaaactt
    18541 tacggggagt agcaaaatat agtggattga aactagaata ctacactgtg ggtttctaaa
    18601 acactcgact tttcacccaa ttttttcata aactagctca atacctcaat agacttcctg
    18661 caaaactaga atcctagttc atgattgata ataccagcaa tcaaattcat tcgtaatccg
    18721 aagcgtttac gatgatttcg ataggttgtt gaaaacattt taaacgtttc tactttggca
    18781 aagatgttct caaccttgct tctctcctta gatagcgcat ggttataggc tttatcttca
    18841 gctattagcg acttgagttt gctggattta cgtggagttt gtgcttgagg atatatcttc
    18901 atgggccctt gataaccact gtcagccaag atttttccag cttgtccgat atttctgcga
    18961 ctcattttga acaacttcat atcatgacaa tagttcacag cgatatccaa agaaacaatt
    19021 ctcccttgac ttgtgacaat cgcttgagcc ttcatagcgt ggcatttttt taccagaatc
    19081 attcgctaat tcttttttta gggcgattga tttgtacttc cgtcgcatca atcattaccg
    19141 tgtcctcaga actgagagga gttcttgaaa tcgtaacacc actttgaaca agagttactt
    19201 caacccattg gctccgacgg attaagttgc tttcgtggat accaaaatca gccgcaattt
    19261 cttcataagt tcgatattct cgcacatatt gaagagtggc cataagaagg tcttctaggc
    19321 ttaatttagg ttttcgtcca ccttttgcgt gtttaagttg ataagctgtt tttaatacag
    19381 ctaacatctc ttcaaaagtc gtgcgttgaa caccaacaag acgcttaaat cgtgaatcgg
    19441 ttagttgttt acttgcttca tcattcatag aactactata ccatatttta tttcgcagga
    19501 agtctaatct aaaaaaggta aatttaagat tggcaatacg aacattaaac agaattccat
    19561 ttctcttttt agaatcaaaa cgacaatatt gtggaatctc tacaggaaca atagactgcg
    19621 acactaaaat atcctccctt agttttactt tactaatagt atataggagg agagccctga
    19681 atcctagacc ctttgttatg atgcgcttac aaacagagtt ggaaaaaatc ttaaaagcag
    19741 aaaaacgcat gatatcagcc cctaacactt gatacaatgc gttttattat gaaaagatct
    19801 actggatttt ttatcaaaat aaacttccag tagtcttttc actttttatt aataagttcc
    19861 ttcttcacct tggctagtca agataacagg tccatctttc gtaatgacaa attggtgttc
    19921 atactgacat gacaatccac cgtcaatggt cttatgcgcc caaccagttt tcatatctgt
    19981 atcaatttcc caatcgcctg tattgatcat tggttcaatg gttaagacca ttccttcacg
    20041 aagacggagt ccacgacctg caataccata gttaggaacc attggttctt cgtgcatagt
    20101 tgggccaaca ccatgaccaa ccaaatcacg cactacaccg taaccacgac tttcagcgta
    20161 ttcttgaata gccgcaccga tatcaccgat acgatttcca acaacagctt gctcaatacc
    20221 cttgtacata gcttctttgg ttacatccat caagtttttg acttcttcgg acggtgtacc
    20281 aacagcataa gcccaacatg agtctgctaa accaccagaa tagctctgag tgtatttttt
    20341 catttgttca acattgttga agtttaattt tgagacattc aggtcggatt tagcaatggg
    20401 acctcccaaa accatatcaa ctttgagcaa atcaccatct ttcaagatat aatgacgagg
    20461 gaaagcgtga gccacttcat cgttaagaga gcaacaggta gcataaggat agtccatcat
    20521 ggcaccgtca accccaatct gaagtggaag gaaattttct tctttacaac gacggcggac
    20581 atattcttca acttcccaca tatctacgcc tggcttaatc aaatcacgta agcctatatg
    20641 aatacttgct agaaaatcac cagccttgtc catagcttcg atttcacgag ctgattttaa
    20701 tgttatcatc ttttctccta gtttctaatt aattttaaca gtcacatttg cttttgaaac
    20761 aatctggtga ccatgataaa tatcgtaatc aattatagct gaccgtctcg tatgatgaat
    20821 aatccgtgcc tgaatgcgca atatatcatc tatctgaaca gcctgcaaaa agtagattag
    20881 catctgctcg ataatgagat tgcgaccact attaacaact aaatcttggg tcatgtgggt
    20941 cagaatttct gccaatacac cattagccaa aactccattt ttttctagca taaagggttc
    21001 cactgtaatg actacttcat catggtgata agagagcttt tgtccaatct gctcagaaaa
    21061 agttggtaga gccgaaactt gagaacggct catcttctcc atgacatctc gtcgcgtcac
    21121 aacgccaagc aaagtttgat tgcttcgaac aactggtacc atttcaaagt cttctgcgat
    21181 catccgttga ctcacattgg caatatttgt cgataatcca accaaaaata gactacgaga
    21241 cataacctta tcaattgtcg tgcttggtga tttatcacca gcgtctctca tggttacaac
    21301 gccaacaacg acctgatgtt gattgataac agggaaacgg ctgctacgat tcttacgaac
    21361 caagtccaaa taatctttaa ctgtatctgt ctctctcaga aaaccatact catgactagg
    21421 gcgataaagt ttctcaactg tcagaatatc agtcttgatt tggacatttg acaaggcttt
    21481 attgatcatg gtcgcgacgg taaaggtatc atgcttactt cttagaacag gaatcccttt
    21541 ttgattggcc agtttaagca catcatcatg aacctgaaat ccccctgtaa ccagaactgc
    21601 attttcattt tccaaggcta gcaactgaat acgggttcgg tctccgacaa tcaagagccc
    21661 cccatcatga aggtaagaca agatattttg ttcagtcatg gcaccaattg aaaacttact
    21721 aaattctctc tctaaacctt cttgcccagc cagaacctca gaagaagtca cttctgcaat
    21781 ttcagcaaac gttaatctct ctatagcaac tttctgggat ttaacacgaa ttgttccact
    21841 tctaggacgg gtctccacaa ttccacggtt ttcagcttct ttaatagccc gataggctgt
    21901 tccatcacta actcctagat gattcgaaat gctacgaaca ctgacccttt tacctactgg
    21961 taattcctcc aaatagctta gaatttcctg atgcttactc attgaactct ccttttacat
    22021 accgaaattc aagataatcc cgcatttttc ttgtgtaacg atcacttccc ttaaactgac
    22081 ggaacaaact aaatccagac ttaagagcaa ctctttggct agctttattt tcaaggtggg
    22141 taatgataaa taattgtttt aagccaaatt cctcaaaaga aagctgacaa atttttctaa
    22201 caacctctgt cataaatcct tgcgaccaag catcttttct caaaaaatag ccaagctcag
    22261 cttctttttt gatttcatct aacttctcaa atttaataga accaatcatt tgttgatttt
    22321 tctggtcaca aattgcccac actcccaaag gggacttcat aaagtaattg gccagtgcat
    22381 attgactttc ttccagactt gcctgcgttg ggaaaataaa ttgaagattt tctggatttg
    22441 aagctatctc gcggaagtcc tgactatcac taaaaaagaa aggacgcaaa tacaagcgat
    22501 ccgtttcaaa aaaagaaaac attgctaatt tggtccaaat attcataaac acctctacgg
    22561 tttaatcctc aacaagtcta atatccgctc ctaaattacg taatttttcg ataatatcag
    22621 aataaccacg taagataaac tcgatattgg taatttcagt tttaccttca gccataagcc
    22681 cagcaatgac tagtgcagcc ccagctctta agtcggtcgc tttaacactg gccccacgta
    22741 aatcacgtcc acccgtgtac aaaatatgac catttgttgt cgaaatatcc gcatccatct
    22801 ttgctagttc aaaaacatga tttacacgtt tttcgtaaat cgtatcgaca attgtaccac
    22861 gaccattcgc tcttagtaaa agaggggtaa gcggttgttg caaatcagtt gcaaagcctg
    22921 ggtaaggagc tgtcttaata ttgattgctt tcaaattaga ctgttcctcg acaaaaatgc
    22981 tgtcttcaga tacagtcatt ctcactccca tttcttccaa cttagcaata aacccttcca
    23041 ggtgttcgta aagaacatta tttatacgaa ttcctttacc aactgcagca gctaaagata
    23101 tatatgttcc agcttcaatg cggtctggaa tcacctgatg acgtgtccca tgtaatcttt
    23161 caacaccatc aataatgatg atattagttc ctgccccacg gatatgggca cccatattat
    23221 tcaagagagt agctacatca ataatctcag gttcacgggc tgcattttca ataatagtac
    23281 gaccatttgc tttaaccgca gcaatcatcg tattaatcgt tgctcccaca ctaaccgtat
    23341 ccatgtaaat acttgcacca tgaagtcctg tatctttagc agataacttc atgttatctc
    23401 cctcgtagct agcagtggca cccatagctt caaacgcctt aaggtgtaag tcaatcggac
    23461 gaggaccaag atcacatcct cccggtagac caactgtcgc ttcaccaaaa cggcctaaga
    23521 ggctcccata aaaatagtaa gatgcacgaa gactgttaat tttaccataa ggcattggaa
    23581 tattttgaac acctcttggg tcaatctcca atacatcgtc ataacgctta acagtagctc
    23641 ccatcaattc catgatttcg acaagactgg ctacatccga aatatctgga acgcaatcca
    23701 aagtcaccac atcatcagcc aagataatag ctggaattaa ggcaacgaca ctatttttag
    23761 caccactaat agtgatttca ccttgcagtg gtaatccacc attgataaca atttttctca
    23821 ttctaagttt tcaatactct ttcaagattt ctaataaggt cttcaaaata aagtatatca
    23881 taaatttatc cttttttcca tccttttcaa ttttattgga taaatcagag ttgacgagtt
    23941 agtaactgaa ttactaatac tcaatgaaaa tcaaagatca aactaggaaa ctagccgcag
    24001 gttgctcaaa acactgtttt gaggttgtag atggaactga cgtagtcagt atcctacaca
    24061 tacgacaagg cgatattgac gtgatttgaa gagattttcg aagagtataa tctttctcga
    24121 atcgctattg cccctttttt atcccctaaa actgagtagg agctaagact agatctctta
    24181 tctaagtatc ggaatacggc tgttcaacta aattttagca agaacttctc ccccttctct
    24241 cagttcacag aagcgtttta tacataccgt cctatcagac agataaccgt gactcaggat
    24301 cacttctatc tgtcgttttc tctttatagt gatagacctc acgggataag tcgtacatct
    24361 ttcctcgtgt atacaagata taagggcgtt aaaaggttac agtgaaaata gaaaatctta
    24421 gtacgaattc aattactctc gtgatttacg catataggtt acgactacct ttttggactt
    24481 tagagtttga tgccctctta tccgctatat acgccttatt atcacgtccc tgttcgtaga
    24541 gccacgattt cgctatccct tcttctgaca attcttattt ccagcgtcag acttaaaacg
    24601 gtctatcccc agaccatttt aatccgctac ctcacgatag tcaggcttgg gaatcgctat
    24661 ttgactcgcc gagaactttg acatcagata tactataaca aattgaaata agaatcaaac
    24721 aatttaattt tgaaattcaa atcaatttat aacaatatag ttgattgaaa ctagaatagt
    24781 acatctctgc ttctaaaaca ttgttagaaa tcgattttac tgtcctgatc gatttgtcct
    24841 gttctcattt cattttacta tattttagaa gatatgctgt gctattccag attcagtttg
    24901 ctatacatgc ctgtcgtacc acaaaatccc gtgtctgatg aggcacggga ttttttagct
    24961 tctttactga gatggatttt cctttttagt tcgtgccaat ctcaaggcac gatttggatt
    25021 gagacgatta aatagttctt ccaatttctt ttcattccaa acgtctgcgg cggaaacaaa
    25081 attaccatcc gcatctcgga aagatatcgg tttatctccc ataccagtct cctagtctac
    25141 aaattcaaac tcaaatttac caatgcggac caaatcccca tctttagctc cacgcgcacg
    25201 aagggcttca tcaaccccca taccacgaag ctgacgggca aatttcatga cagattcatc
    25261 acgatcaaag ttggtcatat taaagagttt catgagtttt tcaccagaaa gtacccatgt
    25321 cgcatcgtca tcacgactaa tttcaaaggc tttttcttct tcgtcaaatc catagtaagc
    25381 ttcttcttcc atatcggact cgtcgtagag caaaaattct ggtgtcttgt ctaacaattc
    25441 agctgtagca tctaaaagtg ttgccagacc ttgcttggtc aatccagaaa ttgggaagat
    25501 agctggtaac tcttcaaatt catcataatt ttcagccaat tttttcttaa attcttcaag
    25561 attttcctga ctctcaggca tgtccatctt attagctaca ataatctgtg gacgctccat
    25621 gaggcgaaga ttgtaagact ccagctcttt attgatagct aggtaatcct catatggatc
    25681 acggccttcg ctagctgaca tatcaatgat gtgaaggata acacgtgtac gctcgatgtg
    25741 acggagaaac tgagttccca aaccaacacc ttgactagcc ccttcaatca aacctggcaa
    25801 gtcggctact gcaaaggatt cacctgattg ggtgcgaacc atacctaaat ttggtacaat
    25861 agtggtaaag tggtaggcac caattttagg cttagctgag gtaataacac ttaaaagtgt
    25921 tgacttccct acagatggga atcctactaa accgacatct gccaagattt ttagttccaa
    25981 ttgtaactca cgttcctgac ctggttctcc attttcagag atttccggtg caggattttt
    26041 tggtgtcgcg aaacgaatat ttccacgtcc accacgacca ccgtgggcaa cgataaattc
    26101 ttgcccatgt tcaatcaaat ctgttaaaac cttgccagtc tccgcatcac gaacagtcgt
    26161 accttgtggt actcgaactc taaggtcctc agcaccacga ccatgcatcc ctttggtcat
    26221 ccctttttca ccagaatcag ccttgaaatg acgattgtag cggaaatcca tcaaggtacg
    26281 tagtccttcg tctacaacga agaccacatt gcctccacga ccaccatcac caccccaagg
    26341 gcctccatta gggacatatt tttcacgacg aaaggcaacc ataccatcgc caccattacc
    26401 agccttgacc ttaatcttag ctgtatctaa aaacatactc attatttctt ctcactttaa
    26461 aaaagggctg ggaaatccca gtcactaaat tttcttgaat ctattttata gattactgag
    26521 ggcaccaatt gcagttgcaa aaattcccaa taaacttgct actagcatga taatcacgat
    26581 aaacaaggtt attttctcaa acatagtttt tttacgattt ccattatctc caaatgccat
    26641 ttttctctcc ttcgttacat tctattttct attatcttag catgaattga gctagttttc
    26701 aatagtcagt tgctactggt gcgataatcc ataaaataat gtaagctaca attccagctc
    26761 cgtaacagca agtaagaaca ccccaaatga cttgaacgat agtcggatcc atatccagat
    26821 aatgggccac cccagcacaa acacccgcaa tctttttatc acgaccgctt ctcattaatt
    26881 gtttttttca tagctgttcc tctttctatt cttcatggtc tttccaatag tctctcatac
    26941 tgatcaactg tgtttttgaa gccttcaaca tgcgtctaga tccttttaca ggtacagcaa
    27001 ccacctgttg cggaagatac aaaactgtcc taaagatacg ctgactgacc gtatcatctt
    27061 tagccaaatc tttctggaag ttatttgaaa taatggcatc cagataaaac tcatgcactc
    27121 gtttcccaaa gttctcagct gaaatctcat acaatttctc tgataaggta tgctcattca
    27181 tgtctggtgt tgcaatcagg gcttccaaaa tagcaccagc caaatcatgt tctccatagt
    27241 acaaggctcc aaacatttta tcactgatga ggttgttcaa ataaggattt ccgtgagcaa
    27301 tgacaggtgt tccactggct aagctttcca agtaggtcaa accttgcgtt tcgcttgtcg
    27361 atgccgaaat gaagaaatcc gccgctttat agtaaagagc cgtctcacta ggagcaatca
    27421 tccctgtaaa gatgactgag tcttgaatct ctaggttctg ggcttgctct ttgaggtcat
    27481 tcagataagg gccatcccca gctactacca gtttaacctt gtcttcctct ttcagaacat
    27541 cagcaaaggc tgctaaaact gcttgaatat ttttttcata ggagattctc gaaagactaa
    27601 gcaacgtctt ttcaccatct tgaatcccta gtttactacg cagttctttc aaattttcct
    27661 gcttgatttc cggacgctca aacttggcta attcaatccc agtaggaatg acccgttttt
    27721 caaccttgac cttataatca gatagcaagt cacggacaat ctcactaggg caaataaccc
    27781 catccacatc atgcaggaaa cctctaacca gatacttgac catactcggc cggatcaata
    27841 tccccttagc aatataatgg acatagtctt catactgggt gtgataggta tggatgactg
    27901 gaattttcaa ttcacgcgca atccaaatcc ccaacaggcc aagagaaaat tctgtctgag
    27961 tatggataat atctagctga tactgtttag caatttcaag tgccttgcta aaacctcggt
    28021 aggcaaagcg acgatcctta aaagcaaaga aaggaacact tggaatgcgg ataatttgcc
    28081 aatcttcgta gcgattgaca tccttatctg tcgtcgtaaa gataaaaaca gcatgtccct
    28141 gcttttcaag ttctgttttc aaggttcgaa tactggtcgc aacaccagaa acctgaggaa
    28201 aataggtatc tgtaaataaa ccaattcgca tagattacct cactttttac tttctcccta
    28261 aagcggcttg tttctcataa aagtccaacc agatttgtaa cagatgctct tcagaatact
    28321 ctctggaaat attcttagcc ttttctttga gatcttttaa gacagcagga tttgcttgat
    28381 attccaaaat agcctctttc atctcttctc tacccgctgt cgcccgataa tttccctcca
    28441 aaatcacctt atagagatct aaatcacgca acataatagg agcctcacaa ctcgcagctt
    28501 ctaaaatagt cataggaaag agctcattgt aactaggcaa caagaaaaga tccgctagag
    28561 catacaattc gcgcatccgc tctggcgata caatgcctgg aaaaatcaaa tttttagggg
    28621 gattttccat aattttctta tagtgttcat aaccatctgt cataccacca aaagagaagc
    28681 caccagccca gataaaggta atctgaggca attcctcagc cagacggata aagtcatcaa
    28741 tccctttacg tttctgaact tgcccagcac ctactacgat aaactgattg tcactaagac
    28801 caagatctgt gcgcagtctg actacctctt cttgtggtag aggatgccat ttttccttgt
    28861 tgacaaagtt aggaatatag gtcacttttt cacgtggaat accagctgct accaaatcct
    28921 caataaacat aggattgacc acaaccaagt gctccatccg gttgtaaaaa gaaaatacat
    28981 agcgtttcac aattcccttt aagaaaaatg gaattttcaa acttccttca agtgtagctg
    29041 gcaagaaatg cacatagcca atctttctcc ctgagcgttt cttttggaag gttgataaat
    29101 aatagggaaa atcaatcgta tgaaagtgag tcacatctgc ctcgattgga agattttctg
    29161 taacaatcaa ttggtccttg gcagcacggt gaagaagacg aactaattca cggtaagcac
    29221 ctgaaactcc ctgtcctgct actttctcac ttgaactcaa catattgatg cgtaatttct
    29281 ttttctccat aactactatt atatcatttt ctttgaataa aatcagcaaa agaaaggaga
    29341 gactagagga aaagagggga aatttcctcg cttttccaac agtctctcac atacttttat
    29401 atgtttactt aatgcctagg gcaatgcggg catagcgact cattttttca actgtccagg
    29461 ctggatacca gactaattta acctcagtat ccgttacttc tggaacctct atcatagcat
    29521 cataaatctg gtctgtcaaa aggtctgcta agggacaacc catagttgtc aaagtcatgt
    29581 caatctctgt ttgccctgtg tcaccgtcaa aacgaatctc atagatcaaa ccaagattga
    29641 caatatcgat tcccaactca gggtcgatga cttcttccaa ggctgttaaa atccgtgttt
    29701 tgatgttttc aatttgctct tctgtataag ccatattttc ctcactctta gtcttcaata
    29761 aaatcacgaa gcggtttgct gcggcttggt tggcgtagtt ttctcaaagc ctttgcttca
    29821 atctgacgga tacgctcacg agttacgtta aagactttcc ccacatcttc aagtgtgcgc
    29881 atttttccat catctagtcc aaaacgtaga cgcagaacat tttcttcacg gtctgtaaga
    29941 gtatctaaga tttcatccaa ttgctcacgc aagacgatac gagtcgtata atccactgga
    30001 ttttcaatca cttcatcttc gataaagtct ccaaggtggc tatcgtcctc ttcaccgata
    30061 ggagtttcaa gagatactgg ttcttgggca atcttcaaga tttcacgaac cttatcaggt
    30121 gtcatatcca ttcgttcagc aatctgttct ggtgtcggat cttgccccaa ttcttgaagg
    30181 agattccgct gttcacgaac caatttattg atagtttcaa ccatgtgaac tgggatacgg
    30241 atggtacgag cttgatccgc aatagcacga gtgatagcct gacgaatcca ccaagttgca
    30301 taagttgaaa acttgaaccc tttagaatag tcaaacttgt caaccgcctt catcaagccc
    30361 atatttcctt cttgaatcaa gtcaaggaac tgcatgccac gaccgacata gcgtttggca
    30421 atggaaacaa ccaaacgaag attggcttcc gcaagacgtt gtttggcttc gatatcgcca
    30481 gcttcaacag ccagtgccaa ctctttctcc tcttcattgg tcaagagagg aacgacccct
    30541 atttctttca agtacatacg gacagggtca ttgaccttag cagaagttga cccaatcaag
    30601 tcctcatcgc tgagttctgg ttcttcttca ttgctaagaa cacgcgcact tggatttcct
    30661 tcgttatctg tgatagaaat gcctgcatcc tgaatccgtt gcaagagatc ttcaatccca
    30721 tcagcgtcca aggtaaaagg aataaccaga cttgcattga tttcatcatc tgttgctgtc
    30781 cctttttgct tatgattacg gataaattct gctacctgta cgtcaaatgt tgttacttct
    30841 ttttgttttg ttgccattat tactccattc ttctcttttg ggaaattaaa cgttccaatt
    30901 cttctagggc tgtatctgta tctcctacat ggctagcttc ctgcaccttc tttttgattc
    30961 tcatattgtc ctgattcaag agagccttgt ttcgagtcat ctctacttca ctaagttcct
    31021 gcggcgatat ctcagcaggc aaatcctgag ctaaaacttg gtaccaagct ctttcaactt
    31081 cctctgtctg ctctgctaaa acttctggag gaagatttcc atactggcca agcaagtcat
    31141 ataagacctg aaattcaggt gtagcaaatg caaagtcttc tcgcaaacgg taatcgttca
    31201 aaacaagagg ggattccatc atccgataga gtagatgggc ttctgccctc ataatagccg
    31261 ataactgctt ggtgataggc atggtgattg gcgtcggtct ggaaattcct tccatgcgat
    31321 tctgcctttg cacctgacga ctctcattaa caatctgctc aatctgggca taatcaaagg
    31381 acgccagact gtcagctaaa atatgaatat aactgttttg agcagcgatg gacttttctt
    31441 gaacaatcaa gggagctatt ttttcaataa actcaatctg agcctgcaga ttttcactgt
    31501 tttcaggttt gtactgatga atgtagaact caatcggact aatacgagtt ttcgttaata
    31561 gataggccaa gtcttctgga ccattttttt gtagatactc atcaggatcc aagttatcag
    31621 gcatgctaac gatttgcaca ggcatatcac caatttcatc caatgctttc aatatcgcgg
    31681 cttgcccagc cttatctcca tcgtaaacaa gaaccaattt cttggttaac cttttcagat
    31741 gctcaacatg ctctcgactc aaggccgttc ccatcgacgc cacagcattt tcgattccag
    31801 cccgataggc tgcaataaca tccatgaatc cttccatcag gtaaatctca ctagtttttc
    31861 cagaagattt ttttgcccta tccatatgat ataattcgta acttttgtta aaaattgcag
    31921 tcgatcggct gtttttatac ttagaagttt gtgaatctgt tttttgccag atacgacctg
    31981 agaaggcaat gacctttcct tggtcatttg tcaggggaaa cataatgcga ttgtgaaagg
    32041 tgtctacaaa ttgattggca tccgagagat aaaacaggcc tgaatccagt aaatcttctt
    32101 cacgatactg atcagacaaa cgttgataga gatagtttcg ttctggaggt gctaaaccaa
    32161 tccaaaaatg tttaagcact tcatctgtca aaccccgctg ataaaggtaa tttctggcct
    32221 cttcgcccat agtcgttgtc atgagaatag catggtaaaa tttagccgca tcttcgtgca
    32281 tatcataaag agcttggtga ggcgaggctg gcttctgttc actataaagc ggtttttcaa
    32341 cctcaatccc gacacgctga cctaatattt ggacagcctc cataaaggta accccttggt
    32401 actcctcgat gaatttaaag acatcacctg agcgaccaca accaaaacag tggtaaaact
    32461 gcttgtcctc tacaacgttg aaagaaggtg ttttttcacc atgaaaagga cagagcccta
    32521 gatagttccg tcctgccttt tgtaaagaaa tcacatctcc tatgacttcc acaatgttgg
    32581 cattgttttt gatttcttca atgacttgtt tgtcaaccat acacaatacc tccatgttat
    32641 catagtttac tttatatagt atactttatt tcagaaaaaa agtaaaccat ttcactcatt
    32701 ttccctactt tattcaaaga gttgataata atcagagatt ttcatttttg ctttttcttc
    32761 ttggtttaaa tcttggataa tccgtccttc tttcatgaca atcaagcgat tgccgtattt
    32821 gagagcatct tccatatgat gagtaatcat aagggctgtt agctgatctt tcttaacaaa
    32881 ttcatctgtc aattccatca aagcaacact agtctttgga tccagggcag cagtatgctc
    32941 atctaacagg agtaattcag gtcgcttcaa ggttgccatc aagagactca aagcctgtct
    33001 ttgtccacct gataagaact caatcggtgt attcaagtgt ttctcaagac catttcctac
    33061 tttttcaatg gttgcctgaa attcatcctt atagctagtc aagcgtcgtg gtaacaatcc
    33121 acgcttttca ccacgaaact tggcgattaa aagattttca gcgaccgtca tacggggagc
    33181 tgtccccatc tttggatctt ggaagacacg agacaggtac ttggcacgct tctcgggtga
    33241 aaacttagtg agttcttcac ctaaaatacg gatagttcca ctagttagtg ataaggtccc
    33301 tgctatagtg ttaaagaggg ttgattttcc agcaccattt ccgcccaaaa tcgtgataaa
    33361 gtcccgttca aaaatttcta aggaaacatc atttaaaata atcttttctt catcaaagcc
    33421 atttttaacg attttggttg cattttttaa ttctacaatt gctgtcattt gcttaacttg
    33481 gctcctttca agattgtttg cttaaatgtt ggaatcatga ggcagactgc taaaatcaag
    33541 gcactgtata aacgaaggta acttgtatta aagccaagtg cgataactgc ccacactaaa
    33601 aattgataag cgatagaacc tacaacgata gtaaccaaac gctctgccaa gctcaaactc
    33661 ttgaaaataa cttctccaat aatcaaactt gcaagcccca caacgataac cccgatccct
    33721 cgagacacat cggcatagcc ttcttgctga gcaatgaggg cacctgcaag ggcaatcaca
    33781 ccatttgata agaccaagcc catgagctcc atgcgtccag tatgaatccc gaaacttcta
    33841 gccatatcag gattatcccc tgtagcaata taggcttgtc cgagtttagt gtccaagaaa
    33901 aagagcatga gagcaataac aatactcaca aagatgagac ctgtcaagag ttgattcaaa
    33961 tccgaatcaa aaggcaaaac atcctgaatt tgcttggttc caagcaggcc taaattcgca
    34021 cgtcccataa tcaagagcat gattgagtga caagaagtca tcaccaaaat ccctgagagc
    34081 aaggttggga tcttcccttt tgtataaaga aggcctgctg ccattccagc caaacaacct
    34141 gctcctacag caacaagtgt cgctaaaaat gggttcacgc ctttggttat caaagtgaca
    34201 gcaacagctc ccccaagagg gaaggaacct tctgtcgtca tatctggaaa gtttaaaatc
    34261 ctaaatgtca taaagattcc cagacctaga atagcccaga caaatccttg agaaataatg
    34321 gaaacaatca tattttattt aatcctttct atattcatct ttttaaaaaa tgggaagagt
    34381 ctcctcctcc ctaccttatt tattcgatga cttgtcctgc ttctttgaga acagactcag
    34441 gaatagtaat acctagttct tgtgctattt ttttattgat gactgactta ccagttgaaa
    34501 agacattgac tggggtatcg gctggttttg cacctttcaa gacttgcaca atcattttac
    34561 ctgttgccac accaaggtca tgttggtcaa ttacaactga tgccaaacca cctacttcta
    34621 ccatagctgt cgcactggga taaattggtt tcttagaact ttgattgcta gagacaaccg
    34681 ttggaaatcc tgatgcaatg gtgttatcaa ttggaaccca aatagcatct accttgctag
    34741 tcataacagt gacagttgag gcaatttcat ttgttgaagg aactgcaaat gtttccactg
    34801 tcagacctgc tttttcagca taagccttaa attcttcgac ctgtgttttt gaattgtctt
    34861 cgctacttga gtaaagagct ccgattgttt tcacattcgg tgtcagagcc ttgatgagtt
    34921 caatttgttg ttgagctgga ttgtggtcag atacccctgt aacgttgcca cctggttttt
    34981 tcaaatcttt aaccaagtta gcaccaattg ggtctgtaat agcggccatg ataactggta
    35041 ggtcttttgt tgcactagcc aacccttggg ctgctggtgt tgcgatacca accacaaggt
    35101 cattcccatt tgcaaccaat tgtttactca ttgtcgcaac cttactttga tcaccttctg
    35161 agttcataaa atcaatttta acttgatcat ctttatatcc ttcttctgca agtccatctt
    35221 ggatcccttt ataaatcaaa tcaagggatg gatggctcac aaattgaagc acaccaatct
    35281 tggttatttt cttctcatcc ttattattct gagcttctga tttattcatt gaagaataaa
    35341 tcaagcttcc tgctactaag actgctaatg cagaaataat tccaattaaa cgtttatttt
    35401 tcattctgtt tctccttttt atttccttta acatatttca cttatctaaa caagcgacgc
    35461 catcgttttc tttccatact aacctccatc aaaaaaatcc tcacacaaaa aacttgtgtg
    35521 aggacgtcga tgcgcggtac cacctcaatt atagggaatt tccctatcgc tcagtctcgc
    35581 aataacgaga tgcactgtaa ggtgtgctta ccgaattttt ataatttcaa attctaaata
    35641 gcattcagcc caattttcat cattgccact tatctgtttt cagctaccac agactctcta
    35701 aaaagtttgt atgattactt ttctgaatgg ttaaattata tcatttttaa actacttgtc
    35761 aatagtctat tagaattttt ttgatattca cttttagcat tcactcttga aaattatcaa
    35821 cctgaattac gcaatcctgt cgcaattccg ttgatggtga tatgaatcaa tcgttcttga
    35881 tcactggaca attctccacg acgttggcgt ttaatcaact ctaactgaat atagttgaga
    35941 atattaaagt aaggcatacg gtaatccaga ctagctttta gatatggatt gtcagctaag
    36001 agttcgtcat gtccttcaat agccaagata acgttcttag taacttgcca ttcatttaaa
    36061 ataatctcat agatggcctt aacttgctcg tcttcacaaa gtttagcata ttcaaaagca
    36121 atattcatat ttgattttga caaaaccata tcaacatttg aaagaagcga ttggaagaaa
    36181 ggccaatttt ggtacatatc tcgtaagata gcaatattct ctggattttt attgataaat
    36241 tccttgaagc ttgaaccaac cccgtaccat ccagggaaca taacacgact ctgtgaccat
    36301 gagaataccc aagggatggc acgcaaacca ccgatttcag taatagtctt acgagcggct
    36361 ggacgagaac caatattaaa acttgaaata gccttgattg gacttgactc gaagaaataa
    36421 tcatagaaat gctcattacc aaagaccaaa tcacggtaga catcgtaact acggtccact
    36481 acttgatcca taatggcttc ataacgattt ggggtattgg tatcactctt cttctgagta
    36541 atcatacggt taatagctgc cgatactagc atttcaaggt tatagtaagc ggcgtctttg
    36601 ttaccgtatt tattcccaat tacttcaccc tgctccgtca agcggatacg atccttgata
    36661 gacttgagcg gttgagatgt aatggcttca taggttggcc caccaccacg accgacagta
    36721 ccaccacgac catggaagaa ggtaacctta acgccaaatt catctccaat agcagtcaat
    36781 tgctgttgag ccttgtagag ggtccaacat gatgacaagt aaccgccatc tttattactg
    36841 tcagagtagc caagcatgat ttcttggtag ttatttcgtg agtcaatcca ttttttggca
    36901 aggctaagag aaagatattt tctcattgtt tcctctgaat gatccaagtc ttcaattgtt
    36961 tcaaaaaggg gaacaatctg aacacgcgcc ctttccgtat ccaccagtcc tacttctttt
    37021 aacagaatag ctaattctag catatcagaa aggctggttg catgtgaaat gatggtctga
    37081 cggatgacat catctcccaa cttatctttc aaaacacgag ccgtcttaaa aatagctagt
    37141 tcttttgcta ataattctga tttttctgcg tgagtcgcag aaagaattcg gggatcttct
    37201 tctaattctt tcaagagaag gtcacacttt tcttcttcgc tcaactcgct ataacgagaa
    37261 tgaattcctg ctgatttcaa gagttctgcc acacaggctt catagacgct agagtcttgt
    37321 cgcatatcaa ttgatgctaa gtaaaaacca aatatctcta ctgcctgcaa taattccaca
    37381 aaatcacctg aaatcaagga ctcgccctta ttttctagta gagaatctcg aatggcaatc
    37441 aaatccttgt agaaatcatt ggcggtttca taacgagtcc caacttcttc atcctcaatc
    37501 agataggttt tagttgcctg aatttttgat tgaatatcaa acaaggcacg acggtaaagc
    37561 tctttttcgc ggtaaatcga gttatccttg gattgacgag ccatttctct gacttgcttg
    37621 ctgacgttga caatgctagt tgaaagagaa aattcacgat aaagttggta aatcttttta
    37681 tcatagtagt tcatgatgac ttcacactga gtgagtgcag actgcttcaa ggtctttgct
    37741 gtaacaaatg gatttccatc acggtcacca cctatccaca tacccatggt gattggttta
    37801 gcctgtttta aattcagacc atgcgcttgc gctaagcgct tatactccgt cgtcaaatga
    37861 ggtacagctt tcaaaaagga gctgttataa tattccatag cattcgtgat ttcgttagtc
    37921 acttttaatt ttttctcacg aatcatgtct gtctgcatga taatttcgat gtaacgacgc
    37981 aaatcattgt accatttatc tttattgatc aaccccaact taacatcacg gtatttacgc
    38041 aaaagactat gaatatgatt tgttaaatcc aacatacttt tgcgttgcac ttgtgttgga
    38101 tgggctgtca aaaccggaac aacattcaag tgttctagga tctcaacggc attttccttt
    38161 tctgctacca atttaatcgt tgtagataat ttacctaaat agtcctgatc aatattattt
    38221 tgatgattga tttcataagc taaatccaca tcctctgaaa tattaatcaa aagaggcaag
    38281 atagagaaat agcgtgaaat atagaccatt tcatcatttg ataagctagt cactagacgg
    38341 tttagacctt gataatcttc ctgcgttgat aattctttca actgtattat tttttcaaag
    38401 gtctctgggg caagcatatt tttagtaata tcttccagta attctgttag aatcaagact
    38461 tcttcttgca caacactttt attactagaa ttttctaatt tttgaagaga cataaactat
    38521 cctttcttta tcctacttca acagacggtt cattggttga ttttccaatt ctcggtacaa
    38581 cttagcgcgt ttttcactgg catcaatatt taagacaaag gctactgcca ctgataagac
    38641 tagaagacta tttccaccct gggataagaa ggggaaggtt actcctgtag atggaatcaa
    38701 gcccgaaatc cctccgatat tgacaaatac ctgaaccaac atcatccctc cgacaccgag
    38761 tgcaaccatg gcattgaaag gattctccgc tcgaataccg accaagataa tccgcaaaat
    38821 catgaaaaac aagagagcta aaataagact ggcaccaaca aagccaaatt cttcaatcac
    38881 gatagaaaag acaaagtctg tatgagcttc tggcaaataa cctcgttttt caatcgagtt
    38941 tccaagacct agaccaaacc aaccaccatt gaccatggca aaataagaat tagctaactg
    39001 gtgacctgca tcagcacgat cggcaaaagg attaaaaaag gcactaaagc gtttggctac
    39061 atagccaaat actggaattt ttgaaaaggt ctcaacaccg attaggctga tagtggtcaa
    39121 gacaaagaca gaagcggcag atacgagcgc cagaatggtt gaaaaccagc gataagcgat
    39181 tccactaact gtatacataa tcaaggaaac caagactaaa atagtcgcat ttcctaaatc
    39241 agggaaaatt cccaaacttc caatcagaac taggagaacg aatcgccaat cattaaaagc
    39301 acggggaagc cattgatttt gagtcaaaac ttgaaaatca taagtagcta tttcttcttg
    39361 ctgtttggag aatcggtgag ctaaatacca aataataatg atttttaagt actcagctgg
    39421 ctgaatagtt actcctgcaa ccgaaatcca accgtatgcc ccgtttactg aaataccaat
    39481 aaaacgagcc aagaacaata aaagcatttc tattaatata actaaaatga ttagtcgctc
    39541 atttctcaaa aaatctagtc tcaatttata aattaaggca atcagtatca aactaacaat
    39601 ccaaaagatt ccttggtttc gaaccaactg caaggcgctc ttgccttctt caattaaaat
    39661 agcactggtg gtcgaataga ccacaatcaa gcccaaaata gataaaagca agtagggaat
    39721 caagatggaa taatttaata agtgcctctt actaatcttc atagtatcac cactctacta
    39781 ggatacagac aaatctgttc caaattccgt tttattttct agttttgatt gctattatac
    39841 cattttttca gaaagaaaaa aactcttacc ttacaatcct tcgaatttta ctgtttttac
    39901 agaattaagg cagaatttga aatcgtaaaa ccattttgaa taagagttgc ttcaacccat
    39961 tgactccgac ggattaagtt gctttcgtga atgccaaaat cagccgcaat ttgttcataa
    40021 gtgctctatt ctcgcatgta ttgaatagtt accataagga gatcgtctag gcttaatttg
    40081 ctttttcgtc caccttttgc gcgtttacgt tgataagctg tttttaacac agctaacatc
    40141 tcttcaaaag tcgtgcgctg aacacctaca aggatcttga aacggctatc gctcaattgt
    40201 ttacttgctt catcattcat agttttattg tattatattt ttgtttcgca ggaagtctat
    40261 tattcctttc gttccaaggt ttttacgctt ttagcaacaa gcttacacac tccacgtgat
    40321 gcgtctgtag aactcaaaag gttcggggaa ccttttgagg attaattgcg tttatctaac
    40381 aaactcacac attcaacgtg gcatgactga tgaagtcatc ataagaattt cacttgctgc
    40441 tcttttacgt tggcaggctg aacggtcaga agtatcattg tatatcattt gtatcatggc
    40501 atataaagta tcgctctatt ttattgatgg ttttaaaatc gctcttatca tggcgagcca
    40561 ttcaaatttg ctccacaaat gaggaaagta ccattttgga ctatttaatt ttcaaagaac
    40621 aaataaaaaa tctcctcatt ttcacactct atgaaattga ggagattatt ttctaaagcg
    40681 aattttatca ggaaaatctg tcaattccaa taggtagtct gtactgacat cgtaaaagtt
    40741 tgagagagtt accaatacat ctgctgttaa cgtatgctca ccccgttcaa ttttagcata
    40801 agctgagttt gtaaacgaaa gtattttagc tatttgtttt tgggtcaaat cgtgatcttc
    40861 cctcaaatct ctcaaacgtc tgtacatcta gctttctcct gagaaaagta tagaccatat
    40921 attgtaacta tttaggatat tgtccaaatc ggacaacagg aattttacca tcctttatat
    40981 agacttttat ttttatgtat caagtttt
//
LOCUS       DAVKQL010000011        40270 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVKQL010000011 DAVKQL010000000
VERSION     DAVKQL010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40270
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.011"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            5..75
                     /locus_tag="VW561_000499"
     tRNA            5..75
                     /locus_tag="VW561_000499"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37..39,aa:Gly,seq:tcc)
     gene            110..183
                     /locus_tag="VW561_000500"
     tRNA            110..183
                     /locus_tag="VW561_000500"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:144..146,aa:Ile,seq:gat)
     gene            266..355
                     /locus_tag="VW561_000501"
     tRNA            266..355
                     /locus_tag="VW561_000501"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:302..304,aa:Ser,seq:tga)
     gene            366..439
                     /locus_tag="VW561_000502"
     tRNA            366..439
                     /locus_tag="VW561_000502"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:400..402,aa:Met,seq:cat)
     gene            443..515
                     /locus_tag="VW561_000503"
     tRNA            443..515
                     /locus_tag="VW561_000503"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:476..478,aa:Phe,seq:gaa)
     gene            528..608
                     /locus_tag="VW561_000504"
     tRNA            528..608
                     /locus_tag="VW561_000504"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:562..564,aa:Tyr,seq:gta)
     gene            613..683
                     /locus_tag="VW561_000505"
     tRNA            613..683
                     /locus_tag="VW561_000505"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:645..647,aa:Trp,seq:cca)
     gene            698..770
                     /locus_tag="VW561_000506"
     tRNA            698..770
                     /locus_tag="VW561_000506"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:731..733,aa:His,seq:gtg)
     gene            784..855
                     /locus_tag="VW561_000507"
     tRNA            784..855
                     /locus_tag="VW561_000507"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:816..818,aa:Gln,seq:ttg)
     gene            865..948
                     /locus_tag="VW561_000508"
     tRNA            865..948
                     /locus_tag="VW561_000508"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:899..901,aa:Leu,seq:caa)
     gene            1055..2332
                     /locus_tag="VW561_000509"
     CDS             1055..2332
                     /locus_tag="VW561_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HEU9942825.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATPEIQAFALNGDEVIISPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            2408..3892
                     /locus_tag="VW561_000510"
     CDS             2408..3892
                     /locus_tag="VW561_000510"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU9942826.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLDF
                     DKLKDTSYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTVELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVCELDS
                     YIEDPHTAVASAVYKKYQSATGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            3953..5233
                     /locus_tag="VW561_000511"
     CDS             3953..5233
                     /locus_tag="VW561_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU9942827.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTILSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLLGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            5237..5857
                     /locus_tag="VW561_000512"
     CDS             5237..5857
                     /locus_tag="VW561_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9942828.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTAILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            6023..7165
                     /locus_tag="VW561_000513"
     CDS             6023..7165
                     /locus_tag="VW561_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU9942829.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEAPKEVASSSEVTKTV
                     IASEEVAPSTGTSVPEEQTAETSSAVAEEAPQETTPAEKQETQASPQAASAVEATTTS
                     SEAKEVASSNGATAAVSTYQPEETKVISTTYEAPAAPDYAGLAVAKSENAGLQPQTAA
                     FKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGI
                     SYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            7488..7913
                     /locus_tag="VW561_000514"
     CDS             7488..7913
                     /locus_tag="VW561_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU9942830.1"
                     /translation="MLEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAIQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIKVKEEMESILTEFERTELSRLLNKLVLGIENIEI"
     gene            7924..8325
                     /locus_tag="VW561_000515"
     CDS             7924..8325
                     /locus_tag="VW561_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEU9942831.1"
                     /translation="MSIITIILTTIVALEHFYIFYLESIATRSDATSRVFNMEKEELA
                     HPSVSSLFKNQGIYKALLGVFLWYGIYFSQNLEIVTIFVLFVIGAATYGSLTADKKII
                     LKQGGSAILALISILLFKIHLKVDSNLANPF"
     gene            8400..9044
                     /gene="pcp"
                     /locus_tag="VW561_000516"
     CDS             8400..9044
                     /gene="pcp"
                     /locus_tag="VW561_000516"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU9942832.1"
                     /translation="MKVLVTGFEPFGGEKGNPTLEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSVTPERVAINQDDARISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKFPYVK
                     AGFMHIPYMMEQVVNRPTTPTMSLVDIRRGIEAAIGAMIEHGDQELKLVGGETH"
     gene            complement(9072..9929)
                     /locus_tag="VW561_000517"
     CDS             complement(9072..9929)
                     /locus_tag="VW561_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEU9942833.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSSFLNP
                     LSRIHGTVLEQPFFSILGQILQAYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            10059..11201
                     /gene="tgt"
                     /locus_tag="VW561_000518"
     CDS             10059..11201
                     /gene="tgt"
                     /locus_tag="VW561_000518"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU9942834.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            11302..13119
                     /locus_tag="VW561_000519"
     CDS             11302..13119
                     /locus_tag="VW561_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEU9942835.1"
                     /translation="MRIKWFSLVRITGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNHEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     ASVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAVAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPIVQTLRPLPYIHAILAAGTGILTIIVCTVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            13272..14423
                     /gene="nagA"
                     /locus_tag="VW561_000520"
     CDS             13272..14423
                     /gene="nagA"
                     /locus_tag="VW561_000520"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEU9942836.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14586..15644
                     /locus_tag="VW561_000521"
     CDS             14586..15644
                     /locus_tag="VW561_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEU9942837.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLINGMQAEYLRVPHADNTLYHTPADLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETAVSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPATFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            15795..16160
                     /locus_tag="VW561_000522"
     CDS             15795..16160
                     /locus_tag="VW561_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEU9942838.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            16236..17177
                     /locus_tag="VW561_000523"
     CDS             16236..17177
                     /locus_tag="VW561_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEU9942839.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKIASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGAIVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            17125..18141
                     /locus_tag="VW561_000524"
     CDS             17125..18141
                     /locus_tag="VW561_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU9942840.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSALLDKQCVTQMRVGLSQGKSFSEMMESLGCSSAIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
                     LGMIVFVSVGVLLALTFYKRSSKMRVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLRPMYQNM
                     EVNF"
     gene            18143..18469
                     /gene="comGC"
                     /locus_tag="VW561_000525"
     CDS             18143..18469
                     /gene="comGC"
                     /locus_tag="VW561_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="comG operon protein ComGC"
                     /protein_id="HEU9942841.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLEKNEDASLSKLKDDGRITEEQVKAYKEYNDKNGGA
                     NRKVND"
     gene            18438..18866
                     /locus_tag="VW561_000526"
     CDS             18438..18866
                     /locus_tag="VW561_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEU9942842.1"
                     /translation="MEEQIVKSMIKAFTMLESLLVLGLVSILALGLSGSVQSTFAAVE
                     EQIFFMEFEELYRETQKRSVASQQKTSLNLDGQTISNGSQKLPVPKGIQVPSGQSITF
                     DRAGGNSSLAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            18871..19131
                     /locus_tag="VW561_000527"
     CDS             18871..19131
                     /locus_tag="VW561_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942843.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVNINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            19094..19555
                     /locus_tag="VW561_000528"
     CDS             19094..19555
                     /locus_tag="VW561_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU9942844.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALLVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWILFVDQLEAELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            19533..19979
                     /locus_tag="VW561_000529"
     CDS             19533..19979
                     /locus_tag="VW561_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU9942845.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQIAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTSKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKAEKKNQKRSLKRKRIPSQFNYNALNPE"
     gene            20082..20669
                     /locus_tag="VW561_000530"
     CDS             20082..20669
                     /locus_tag="VW561_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9942846.1"
                     /translation="MKHDFNHKAETFDSPKNIFLANLVCQAVEKQIDILSDKEILDFG
                     GGTGLLVLPLAKQAKSVTLVDISEKMLEQARLKVEQQAIKNIQFLEQDLPKNPLEKEF
                     DCLAVSRVLHHMPDLDAALSLFHQHLKEDGKLIIADFTKTEANHHGFDLAELENKLIE
                     HGFSSVHSQILYSAEDLFQGNHSEFFLTVAQKSLA"
     gene            20730..21683
                     /locus_tag="VW561_000531"
     CDS             20730..21683
                     /locus_tag="VW561_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9942847.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEMDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKEWLKEEASLVAMISLPENLFANTKQSKTIFI
                     LQKKNEIAVDPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            21734..22924
                     /locus_tag="VW561_000532"
     CDS             21734..22924
                     /locus_tag="VW561_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEU9942848.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKYIGQYLAVLNGADAIVFTAGVGE
                     NAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            23073..23444
                     /gene="rnpA"
                     /locus_tag="VW561_000533"
     CDS             23073..23444
                     /gene="rnpA"
                     /locus_tag="VW561_000533"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEU9942849.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            23419..24243
                     /locus_tag="VW561_000534"
     CDS             23419..24243
                     /locus_tag="VW561_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU9942850.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYS
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            24262..25248
                     /locus_tag="VW561_000535"
     CDS             24262..25248
                     /locus_tag="VW561_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEU9942851.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERYASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQTIIDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNCYSRTFYITINVNDYVEHRAEVLQTYAQKL
                     AIRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            25299..25922
                     /locus_tag="VW561_000536"
     CDS             25299..25922
                     /locus_tag="VW561_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEU9942852.1"
                     /translation="MLNKIRDYLDFSGLQYRSPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            26053..27510
                     /locus_tag="VW561_000537"
     CDS             26053..27510
                     /locus_tag="VW561_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9942853.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIVAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            27533..27814
                     /locus_tag="VW561_000538"
     CDS             27533..27814
                     /locus_tag="VW561_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9942854.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            27891..28376
                     /locus_tag="VW561_000539"
     CDS             27891..28376
                     /locus_tag="VW561_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9942855.1"
                     /translation="MNLKQALIDNDSIRLGLEANDWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            28393..29058
                     /locus_tag="VW561_000540"
     CDS             28393..29058
                     /locus_tag="VW561_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEU9942856.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            29062..29925
                     /locus_tag="VW561_000541"
     CDS             29062..29925
                     /locus_tag="VW561_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEU9942857.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIS
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAVQEAQAF
                     LYPLIKKAGLM"
     gene            29927..30631
                     /locus_tag="VW561_000542"
     CDS             29927..30631
                     /locus_tag="VW561_000542"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEU9942858.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            30812..32485
                     /locus_tag="VW561_000543"
     CDS             30812..32485
                     /locus_tag="VW561_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9942859.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALVDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNESKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            32597..33688
                     /gene="ulaG"
                     /locus_tag="VW561_000544"
     CDS             32597..33688
                     /gene="ulaG"
                     /locus_tag="VW561_000544"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEU9942860.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            33802..35778
                     /gene="tkt"
                     /locus_tag="VW561_000545"
     CDS             33802..35778
                     /gene="tkt"
                     /locus_tag="VW561_000545"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU9942861.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDTNRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            35895..36194
                     /gene="yajC"
                     /locus_tag="VW561_000546"
     CDS             35895..36194
                     /gene="yajC"
                     /locus_tag="VW561_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU9942862.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            36241..36669
                     /locus_tag="VW561_000547"
     CDS             36241..36669
                     /locus_tag="VW561_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEU9942863.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            36671..37081
                     /locus_tag="VW561_000548"
     CDS             36671..37081
                     /locus_tag="VW561_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942864.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            37381..40032
                     /gene="adhE"
                     /locus_tag="VW561_000549"
                     /gene_synonym="adhC"
     CDS             37381..40032
                     /gene="adhE"
                     /locus_tag="VW561_000549"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEU9942865.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT        12212 a         7204 c         9090 g        11764 t
ORIGIN      
        1 tttagcgggt gtagtttagt ggtaaaacta cagccttcca agctgttgtc gcgagttcga
       61 ttctcgtcac ccgctttgaa ctttgttctt tgtaccaagt ttttgacttg ggcgcgtagc
      121 tcaggtggtt agagcgcacg cctgataagc gtgaggtcgg tggttcgagt ccactcgtgc
      181 ccatagtgtt tagtccatta ctaggggatt ggaatattat ctgttcacta agaggacacg
      241 ggcttgttcc cgtataaact attttggagg attacccaag tccggctgaa gggaacggtc
      301 ttgaaaaccg tcaggcgtgt aaaagcgtgc gtgggttcga atcccacatc ctccttttat
      361 attaacgcgg gatggagcag ctcggtagct cgtcgggctc ataacccgaa ggtcgtaggt
      421 tcaaatcctg ctcccgcaat aaggctcggt agctcagttg gtagagcaat ggattgaagc
      481 tccatgtgtc ggcggttcga ttccgtctcg cgccatttat atattttgga agggtagcga
      541 agaggctaaa cgcggcggac tgtaaatccg ctccttcggg ttcgggggtt cgaatccctc
      601 cccttccatt ttacgggcat agtttaaagg tagaactaag gtctccaaaa ccttcagtgt
      661 gggttcaatt cctactgccc gtgttaatag aattatggcg ggtgtggtga agtggttaac
      721 acaccagatt gtggctctgg catgcgtggg ttcgatcccc atcactcgcc tattttatat
      781 tattggggta tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga
      841 atccagctac cccagttact atttgccggc gtggcggaat tggcagacgc gctggactca
      901 aaatccagtg tccgcaagga cgtgccggtt cgaccccggc cgccggtata gtatagtgtt
      961 aggaacgttg ttattcttcg ttcctttttt atattatttt tggtataatt atagttattc
     1021 aaattttatt tagattaaga aagtgtaggg tagtatgtct tgttctatcg atttattaaa
     1081 acatcggtat ttgaaaaata ttaaagaaaa tcctgaattg tttgtcggaa ttgagttgga
     1141 gtatcctgtt gcaagtttag aaggggatgc tacagatgtt gaagttataa aggatctatt
     1201 tcattattta gtttctactt tggatctcac cgtagcaaag gtagatgatt ttggcaatct
     1261 gatccagtta gtagatccga taagtcagga tgctatttta tttgaagttt cctatacaac
     1321 gattgagttt gcatttggta aggctgaaac gattcaagag gtcgaaaatc gtttcaataa
     1381 ttatatgaat gtaattcaga gaaagttagc tgaatcaaat catgctattg ttggctgtgg
     1441 tatccatccc aactgggata aaaatgagaa ttgtccagtg gcttatccac gctatcagat
     1501 gttgatggat tatttgaatt tgagtagaaa tattattaaa tcagatttac atcatttccc
     1561 tgaatatggt acttttatct gtgggagcca ggttcagctg gatatttcaa aaaccaacta
     1621 cttacgggtg attaatgctt ttactcaaat tgaagcggct aaggcttatt tatttgcaaa
     1681 ctctgaattt tcgggtgcgg attgggatac gaaaatttca agggatattt tctgggaaga
     1741 atctatgcat ggtatctatc cagagaatgt tggggtcaat gctagactcc ttaatgatga
     1801 agctgatttt tttgactatc taaatcattc tgcgattttt actgcggaac gtgatgggca
     1861 gacctattat ttttatccta ttcaggctgg ggactatttg gctacgcctg aaatccaagc
     1921 atttgctctg aatggggatg aggttattat ttccccccaa gagaaggatt ttgaaactca
     1981 tcgtagttac cagtaccaag acttaacgac tcgaggaaca gttgagtttc gtagtgtgtg
     2041 tacacagcca cttgatagga cttttgcttc tgcagctttt cacttgggat tattggttaa
     2101 tttagacaag ttagaagctt acttagaaac agcacctttc tttaaagtat ttggttatga
     2161 ttacaagtct ttaaggagac aattttctaa gaaaaatctt acagatgagg aagaaactac
     2221 gattattgaa ttttccaaag acttactcct actagctgag gagggactag tggtgagaaa
     2281 taaggaagaa atgacctatt tacagccttt gagagaagaa ttgagcctat aatttctctt
     2341 ataaagggag aattttctga aaaatcatga tataatggac gagactatag ataaaggata
     2401 gagagtaatg acattagttt atcaatcaac gcgtgatgcc aacaatacag taactgctag
     2461 ccaagcaatt ttgcaaggtt tggcgacgga cggcggtttg tttacaccgg ttacttatcc
     2521 aaaggtagat ttggactttg acaaattgaa agatacttct taccaggaag ttgctaagct
     2581 agttttgtca gcatttttag atgactttac agctgaggag ttggactact gtatcaacaa
     2641 tgcctacgat agcaaatttg atactccagc tattgcacca ttagtgaaat tagatgggca
     2701 atacaatttg gaacttttcc atggttcaac gattgccttt aaggatatgg ccttgtctat
     2761 tttgccatac tttatgacga ctgctgctaa gaaacatggt ttggagaaca agattgttat
     2821 cttgacagcg acatctggtg acacggggaa agctgctatg gcggggtttg cggatgtgcc
     2881 tggtactgag attatcgtct tttatccaaa ggatggtgtc agcaagattc aagagttaca
     2941 aatgaccact caaactggcg acaatactca tgttattgct attgatggta actttgacga
     3001 tgcgcaaaca aatgtgaagc acatgtttaa cgacgtggct cttcgtgaaa aattgactac
     3061 caacaagttg caattttcat cagctaactc tatgaacatt ggtcgtctgg tgccacaaat
     3121 tgtttattat gtttatgctt acgctcaatt ggttaagact ggtgaaattg tagctggtga
     3181 aaaggttaac ttcacagtac caacaggaaa ctttggaaat atcttggctg ccttttatgc
     3241 caaacaaatt ggtttgccag ttggtaaatt aatctgtgct tcaaatgaca ataatgtctt
     3301 gacagacttc tttaaaacac gtgtctatga caaaaaacgt gagtttaagg taacaaccag
     3361 cccatctatg gatatcttgg tatcttcaaa cttggagcgc ttgattttcc atcttttggg
     3421 aaataatgct gaaaagacag ttgaacttat gaatgccttg aacacgcaag gacaatataa
     3481 gttgacagac tttgatgcag agattttgga cctctttgca gctgaatatg cgactgagga
     3541 agaaacggca gcagagatca agcgtgtttg tgagttagat tcttatatcg aggaccctca
     3601 tacagctgtt gcttcagcag tttataaaaa ataccaatcg gccactggag atgtaactaa
     3661 gacagtgatt gcttcaacag ctagtccata caagttccca gtagttgcag tagaagctgt
     3721 aactggaaaa gcaggtttaa cagactttga agccttggct caattacatg aaatctcagg
     3781 cgttgcagtg ccaccagcag ttgatgggct tgaaatagct ccaattcgtc acaagacaac
     3841 agtggcagct gctgacatgc aagcagcggt tgaggcttat ttaggacttt aagacagagg
     3901 gagcaaactc ggttgggaaa ccaactgagt ttcttttcat caggaggaga gattgtttaa
     3961 gaaaaataaa gacattctta atattgcatt gccagctatg ggtgaaaact ttttgcagat
     4021 gctaatggga atggtggaca gttatttggt tgctcattta ggattgatag ctatttcagg
     4081 ggtttcagta gctggtaata ttatcaccat ttatcaggcg attttcatcg ctctgggagc
     4141 tgctatttcc agtgttattt caaaaagcat agggcagaaa gaccagtcga agttggccta
     4201 tcatgtgact gaggcgttga agattacctt actattaagt ttccttttag gatttttgtc
     4261 catcttcgct gggaaagaga tgataggact tttggggacg gagagggatg tagctgagag
     4321 tggtggactg tatctatctt tggtaggcgg atcgattgtt ctcttaggtt taatgactag
     4381 tctgggagcc ttgattcgtg caacgcataa tccacgtttg cctctctatg ttagtttttt
     4441 atccaatgcc ttgaatattc ttttttcaag tctagctatt tttgttctgg atatggggat
     4501 agctggtgtt gcttggggga caattctgtc tcgtttggtt ggtcttgtga ttttgtggtc
     4561 acaattaaaa ctgccttatg ggaagccaac ttttggttta gataaggaac tgttgacctt
     4621 ggctttacca gcagctggag agcgacttat gatgagggct ggagatgtag tgatcattgc
     4681 cttggtcgtt tcttttggga cggaggcagt tgctgggaat gcaatcggag aagtcttgac
     4741 ccagtttaac tatatgcctg cctttggcgt cgctacggca acggtcatgc tgttggcccg
     4801 agcagttgga gaggatgatt ggaaaagagt cgctagtttg agtaaacaaa ccttttggct
     4861 ttctctgttc ctcatgttgc ccctgtcctt tagtatatat gtcttgggtg taccattaac
     4921 tcatctctat acgactgatt ctctagcggt ggaggctagt gttctagtga cactgttttc
     4981 actacttgga acccctatga cgacaggaac agtcatctat acggcagtct ggcagggatt
     5041 aggaaatgca cgcctccctt tttatgcgac aagtataggc atgtggtgta tccgcattgg
     5101 aacaggatat ctgctgggga ttgtgcttgg ttggggcttg cctggtattt gggcagggtc
     5161 tcttttggat aatggttttc gctggttatt tctacgctat cgttaccagc gctatatgag
     5221 cttgaaagga taggaaatgc aaaaaacagc ttttatttgg gatttagacg ggactttatt
     5281 ggactcttac gaagcgattt tatcagggat tgaggagact tttgctcagt tttctattcc
     5341 ttatgataag gagaaggtga gagagtttat cttcaagtat tcggtgcaag atttgcttgt
     5401 gcgggtggca gaagatagaa atctggatgt tgaggtgcta aatcaggtgc gtgcccagag
     5461 tctggctgag aagaatgctc aggtagtttt gatgccaggt gcgcgtgagg tgctagcttg
     5521 ggcagacgaa tcaggaattc agcagtttat atatactcat aaggggaaca acgcttttac
     5581 cattctcaag gacttggggg tggaatccta ttttacagcg attttaacca gtcagagtgg
     5641 ctttgtgcgg aagccaagtc cagaagcggc tacctatctg ctagataagt atcagttgaa
     5701 ttctgataat acttattata taggggatcg gactctggat gtggaatttg cccagaatag
     5761 tgggattcaa agtatcaact ttttagagtc tacttatgaa gggaatcaca ggattcaagc
     5821 gttagcagat atttcccgta tttttgagac taagtgataa aaagattgtg tcagttttgt
     5881 gacagagacc taacaaacta tttcaagtaa cctagtttgt tacaaggaat agacagttct
     5941 gttaaatagg cccgagaggg ctttttttct acattttttg tgttatcata gacaggtact
     6001 catttgaaag gaatttgaaa gaatgaagaa aagaatgtta ttagcgtcaa cagtagcctt
     6061 gtcatttgcc ccagtattgg caactcaagc agaagaagtt ctttggactg cacgtagtgt
     6121 tgagcaaatc caaaacgatt tgactaaaac ggacaacaaa acaagttata ccgtacagta
     6181 tggtgatact ttgagcacca ttgcagaagc cttgggtgta gatgtcacag tgcttgcgaa
     6241 tctgaacaaa atcactaata tggacttgat tttcccagaa actgttttga caacgactgt
     6301 caatgaagca gaagaagtaa cagaagttga aatccaaaca cctcaagcag actctagtga
     6361 agaagtgaca actgcgacag cagatttgac cactaatcaa gtgaccgttg atgatcaaac
     6421 tgttcaggtt gcagaccttt ctcaaccaat tgcagaagct ccaaaagaag tagcatcaag
     6481 ttcagaagtt acaaagacag tgattgcttc tgaagaagtg gcaccatcta cgggcacttc
     6541 tgtcccagag gagcaaacgg ccgaaacaag cagtgcagtt gcagaagaag ctcctcagga
     6601 aacgactcca gctgagaagc aggaaacaca agcaagccct caagctgcat cagcagtgga
     6661 agcaactaca acaagttcag aagcaaaaga agtagcatca tccaatggag ctacagcagc
     6721 agtttctact tatcaaccag aagagacgaa agtaatttca acaacttacg aggctccagc
     6781 tgcgcccgat tatgctggac ttgcagtagc aaaatctgaa aatgcaggtc ttcaaccaca
     6841 aacagctgcc tttaaagaag aaattgctaa cttgtttggc attacatcct ttagtggtta
     6901 tcgtccagga gacagtggag atcacggaaa aggtttggct atcgacttta tggtaccaga
     6961 acgttcagaa ttaggggata agattgcgga atatgctatt caaaatatgg ccagccgtgg
     7021 cattagttac atcatctgga aacaacgttt ctatgctcca ttcgatagca aatatgggcc
     7081 agctaacact tggaacccaa tgccagaccg tggtagtgtg acagaaaatc actatgatca
     7141 cgttcacgtt tcaatgaatg gataaacccg acttgataac atcattttga cgaatgagat
     7201 ctagctttcg tgatggaaag cgattctcgt tcgttttttc tttgtcatac tcttcgaaaa
     7261 tctcttcaaa ccacgtcagt tttatctgaa acctcaaagc tgtgctttga gcaacctgcg
     7321 actagcttcc tagtttgctt tttgattttc attgagtatc aatttgaatg gaaaatggaa
     7381 agttatcatc ttgtaatgag ttaagcaaca ttcttgcaat ctattttact ttatatcaca
     7441 attaattagt caaatattga taaatcaata aaaagagagg ggaagaaatg ctagagattc
     7501 aagatttact gtatcaactc cgcttgtctg agcaagcgag tacgcaattg tttgaaaaaa
     7561 ggcttgggat tagtttgaca cggtatcaga ttttactgtt tttgctggag cattctcctt
     7621 gtaaccaaat ggcgattcag gagcgtttga aaattgatca ggctgctttg acacggcatt
     7681 tcaagatttt ggaaacggaa ggtttggtgg agcgtcatcg taatcctgaa aatcagcggg
     7741 aagtgttggt agaggctgcg aagtatgcca aggagcagtt agtggtgaat ccccctctgc
     7801 aacatatcaa ggttaaggaa gagatggaaa gtatcttaac agagtttgag agaacagaac
     7861 tcagccgttt attaaataaa ttggttttgg gtattgaaaa tatagaaatt taaggagaaa
     7921 tagatgtcaa ttattacaat tattttaaca acgatcgttg ctttggagca tttttacatt
     7981 ttttatttgg aaagtattgc tacgcgatca gatgcgacta gtcgtgtatt taatatggaa
     8041 aaggaagaat tggctcatcc gtcagtaagt tcattgttca aaaatcaagg gatttataag
     8101 gctctgctag gagtctttct ctggtatggc atttatttct cacagaattt agaaattgtg
     8161 actatttttg tcttatttgt gattggtgct gcgacttacg gctctttaac agcggataaa
     8221 aaaattattt tgaagcaagg tggatcagct attttggcct tgattagtat tttactcttt
     8281 aaaatacact tgaaggtcga ttctaatctc gctaatcctt tttaatccag aataagggaa
     8341 atatgttata cttgttttta agaaaaaagt ctcattgaat tggttttgag gagttagaaa
     8401 tgaaagtatt agtgacaggt tttgagccct ttggagggga aaagggcaat ccaactttgg
     8461 aggccattaa aggtttacca gctgaaatcc atggtgctga ggtccgttgg ctagaggtgc
     8521 cgacagtttt tcacaaatct gctcaagtat tggaagaaga gatgaatcgt tatcaacctg
     8581 actttgtcct ttgtattggg caagctggtg gaagaactag tgtgacacct gaacgagtgg
     8641 ccattaatca agacgatgca cgcatttctg ataacgaaga taatcaaccg attgaccgtc
     8701 ccattcgccc agatggtgct tcggcctact ttagtagttt gccgattaaa gcgatggttc
     8761 aagctataaa aaaagagggc ttaccggcct ctgtttccaa tacggcaggg acttttgtct
     8821 gcagccattt gatgtatcag gctctctatt tggtagaaaa gaaatttcca tatgttaagg
     8881 caggttttat gcatattcct tatatgatgg aacaggtggt gaacagaccg actactccaa
     8941 ctatgagttt agtggatatt cggcgaggga tagaagcagc aatcggcgct atgatagaac
     9001 atggagatca ggaactcaag ttggtaggcg gagaaactca ttgatagaaa aaagcttgag
     9061 gggaaaaacc ttcaagcttt tggacgtttt cgagccaata ctgctcggta aaacataatt
     9121 ttagtgcatt ggatataagg taggagtgaa aaactagcaa tgccaaaggt aatccaattg
     9181 aggaagtacc aaggaagaag ctgtaaatct aggacaaagt gctggaactt gtagcccttc
     9241 ataaaggaac ggctagtttt taggattcgt cttggtggga cctgtcctag gtctagacta
     9301 taacagagaa gaaattccac ctgtgaatag gcataatact gtggaatata gaggatattt
     9361 cctacaatga tcaagatgag acttgcaaga aagtagagtc caaagaccat gaggaaacgc
     9421 tcggtttcaa ctgatgagag atctagattt ggaaactcag gatgtagggt gacgaatttt
     9481 ttggctaaaa agctactata aaagaggaga taaatcccaa gtaaattagg gatactccat
     9541 aaaaagagat agaaacgttt gagaagtagg gtcaaaaagg tttgagaaaa gcgctcctca
     9601 tcaaagagag ctaggctgtt ttttacagat ggctccgttt tagaatcttt catgagtgtc
     9661 agtgttgcat agacggaact ggtcaaaaga atagtcccga taaaggagac tagtagagga
     9721 aagaggtagg cttgaagtat ttggccaagt atgctgaaaa atggctgttc taaaacagtc
     9781 ccgtggatcc gagataaggg attaagaaaa ctagataaga tgaccagcat actgggaagg
     9841 atatagagga gaaagagacg gggggtgtca gcctgaaaat gttttgactc ctgacgaatt
     9901 gtttttaaat caatttttgg atagttcatt ctcttattat accatagttc ttatacatag
     9961 ttcgtgacag ttcctacttt ttttgataaa atcatacagt gtgtccttgg gcacactgta
    10021 tgaactggga ctgtctttcc cagcttcgga ggtaaaaaat gtcagattca ccaatcaaat
    10081 atcgtttgat taagaaagaa aaacacacag gagctcgtct aggagaaatc atcactcccc
    10141 acggtacctt tccgacacct atgtttatgc cagttgggac acaagccact gtcaaaactc
    10201 agtcacctga agaattgaag gagatgggtt cgggaattat cctatcaaac acttatcatc
    10261 tctggcttcg ccctggagat gaactcattg cacgcgctgg tggtctccac aagttcatga
    10321 attgggacca gcctatcttg acagatagtg gtggttttca ggtttattct ttagcagata
    10381 gccgtaatat cacagaagaa ggagtaacct ttaaaaatca tctaaatggt tctaagatgt
    10441 tcctatcccc agaaaaagcc atctctattc agaataatct gggttcagac atcatgatgt
    10501 cctttgatga atgtcctcag ttttatcaac cttatgacta cgttaagaaa tcgatagagc
    10561 gtaccagccg ttgggctgag cgtggtttga aggctcaccg tcgtccacat gaccaaggtt
    10621 tgtttggaat tgtgcaaggt gcaggatttg aagaccttcg ccgccaatca gctcatgatc
    10681 ttgtcagcat ggatttctca ggctactcta tcggtggttt ggcagtggga gaaacccatg
    10741 aagagatgaa tgcggtcttg gactttacaa ctcaactgct gcctgaaaat aaacctcgtt
    10801 atctgatggg tgtgggagcg ccagatagct tgatcgatgg ggtcattcgt ggggtggata
    10861 tgtttgactg tgtcttaccg actcgaattg ctcgtaacgg gacttgtatg accagtcaag
    10921 gacgtttggt tgtgaaaaat gcccagtttg ctgaggactt tacgccactg gatcctgagt
    10981 gtgattgcta cacatgtaat aactatacac gcgcttacct tcgtcacctg ctcaaggctg
    11041 atgaaacctt tgggatccgc ttgactagct accacaatct ttacttcttg cttaacctga
    11101 tgaagcaagt gcgacaagcc atcatggatg acaatctctt ggaattccgt gagtattttg
    11161 tggaaaaata tggctataat aagtcaggac gtaatttcta aaatggaatt gatataagct
    11221 aaaaatccta agttttctct taggattttt cttctttttt tgatagaata aagtgtacaa
    11281 tgaaaggaag aataaactcg tatgcgcatt aaatggtttt ccttggttag gattacaggt
    11341 ttacttttgg tactcttgta tcacttcttt cagacgatct ttcctggagg atttttcggg
    11401 gtagatgtct ttttcacatt ttcaggtttc ctgattacag ctctactcat tgaagaattt
    11461 tctaaaaacc atgagattga tttgatagga ttttttagaa gacgctttta tcggattgtg
    11521 ccacctgtgg ttttgatggt cttggtgacc atgcctttta ctttcttggt tcgccaagac
    11581 tatgttgctg gaattggtgg ccagattgcg agcgtcttag gctttatgac caacttctat
    11641 gaactcctaa caggtgggag ttatgaatct cagttcattc ctcatttgtt tgttcataat
    11701 tggagtttgg cagttgaggt tcactactat attctttggg gattggcagt ttggttctta
    11761 tccaaacaag ctaaatcaaa tggtcagttg aaggggatgg tctttctctt atctgctgtt
    11821 gccttcttga tcagtttctt ctccatgttt attggtagtt ttctagtgac ctcttattcc
    11881 tctgtttatt tctccagttt aactcatgtc tatccattct ttttgggaag tatgttagca
    11941 actattgtag gcgttcgtca gacgacttcc ctcgtcaagc agttggataa aatctgggat
    12001 ttacgaaaga ctttggtagt ttttggagga ggctttggtt tcctagttct tttgactttc
    12061 tttgtcaaat tcacttatct ttttgcctat cttatcggct tcttacttgc cagtcttgca
    12121 gctcttgcca tgattctggc ggcgcgtgtc ttacatgaaa agacacatca tatacaggag
    12181 ccgaagatta tcagcttttt agcggatact agctatgcgg tttatctttt ccattggcct
    12241 ttctatatca ttttctcaca gttgacatca aatcttcttg ctgtattact gactctgatt
    12301 tgttcttatg gctttgccag tctgtcattt tatgtattgg aaccttggat tgcaggcaag
    12361 aacacaccta ttgtccaaac ccttcgtccc ctgccttata ttcacgcaat tcttgcagca
    12421 ggtacaggaa tcttgaccat cattgtctgc acggtgacct tgttggcacc acaagtggga
    12481 gcgtttgaga cagacttgac tgtcaatggc ttgaagcaag ctgcaacaaa tattggccag
    12541 accaaggtga tggcagaacg ggcagatgca aacagtttgg gaattgctga tggcactatg
    12601 ttaattggtg actcagtggc tttaagggca aatacagcac tacagacagc tcttcctgga
    12661 gcacagatta acgcgcaggt cagcgtaaca accaagaccg caaatgaaat catgctaaat
    12721 aatagccaga ataaattttt acctaagacg gtggtcattg cgactggggt aaataatcct
    12781 gagaattaca aggatgactg ggacagtatc gtgaaaaatc ttcctaaggg acaccatatg
    12841 attttggtga ctccttatga gggagataag acaaaagaga cctatgccat cgttgagaag
    12901 gctgctgcct atatgagaga attggcagag aagacacctt acattacgat agcagattgg
    12961 aatcaagttg cgaaagagca tccagaaatt tgggctggaa cagaccaggt tcatttcggg
    13021 agtgagagta gcactatcga agcaggagca aaattgtatg cagatacgat tgccacagct
    13081 ttgcagacag ctcaagacaa gccggttaaa tcaaaataac ttgtattaaa aaagagaaga
    13141 gttggagaaa tccagctctt ttaaaatatc tccaaaaaat aggtctatac catttacaaa
    13201 taaaaaagaa aggtttataa tgtaattgac ataataaatt gtagaatcaa tcttttaagg
    13261 aggttaacat tatgcctaac tatattaaag cggatcagtt tttctaccca cacggagttc
    13321 gtcgaggtgg ttacttggaa cttgtggacg gcaagtttgg gaaacatgta gagcagattc
    13381 ctgaaggggc tgaggtgatt gactatacag gttatagcat tgccccaggt cttgtggata
    13441 ctcatattca tggatatgca ggtgtagatg tgatggacaa caacattgaa ggtacattgc
    13501 atactatgag tgaaggactt cttagtaccg gtgttaccag tttcttaccc acaactttaa
    13561 cagccactta tgagcaattg cttgcagtca ctgaaaatct tggaaaccat tataaagaag
    13621 caacaggtgc taagattcgt gggatttatt atgaaggtcc atatttcaca gaaactttta
    13681 agggggcaca aaatccaact tatatgagag acccgggtgt tgaggagttt cattcttggc
    13741 aaaaagcggc aaatggcttg cttaataaaa ttgcccttgc accagaacgt gatggggtgg
    13801 aagactttgt tcgtacagtt acgggcgaag gtgtgacggt tgctcttgga cattcaaacg
    13861 cgacttttga tgaagccaaa aaagcagtcg atgctggagc gagtgtttgg gtgcatgcct
    13921 acaatggaat gcgtgggttg actcaccgtg aattgggtat ggttggagcc atgtaccaat
    13981 tgccacatac ctatgcagag ttgatctgtg atggtcacca cgtagatcca aaggcttgcg
    14041 aaattcttat caaacaaaaa ggaacagaaa acatcgctct tatcacagac tgtatgacag
    14101 ctgggggatt ggaagacgga gattatatgt tgggagaatt cccagttgtc gttgcaaatg
    14161 gaactgcacg cctcaaatcg acaggtaact tggcaggttc tatcctcaaa ctcaaagatg
    14221 gtttgaagaa tgtggtcgaa tggggaattg cgaatccgca tgaagcagtc atgatggcca
    14281 gcttcaaccc agctaaatcc gttcacatcg atgacgtctg tggccaaatc cgtgaaggct
    14341 acgacgctga cttcatcgta ttagataaag atttggaatt ggtagcaacc tacctagatg
    14401 gcgtaaaacg ttatcaagca taagagagaa agcagaagtt agagactaac ttctgctttt
    14461 ttatgcaggg tactttactg gtaaataaaa agttaaaaaa atcacaaaaa aagcttgaag
    14521 aaacaaagaa aaaggcttac aattgaacta taaatattac aaataaaaaa acggaggagt
    14581 gctttatgaa agcctatact tatgttaaac caggacttgc ttcttttgtt gatgtagaca
    14641 aaccagttat tcgcaagcca acagatgcta ttgtgcgtat tgtaaaaacc actatttgtg
    14701 gaacagacct ccatattatc aaaggggatg ttcctacttg ccaaagtggt accattcttg
    14761 gccacgaagg gattgggatt gttgaagaag ttggggaagg agtttctaac ttcaaaaaag
    14821 gtgacaaggt cttgatttct tgcgtctgtg cctgtggtaa atgctactac tgtaaaaaag
    14881 gaatttatgc tcactgtgaa gacgaagggg gctggatttt tgggcatttg attaatggta
    14941 tgcaggctga atacctacgt gtacctcatg cagataatac tctttaccat acgccagcag
    15001 acttgtcaga cgaagctttg gttatgctat cagacattct gcctactgga tatgaaattg
    15061 gtgtcttaaa agggaaagta gaacctggtt gcagcgtagc cattattggt tcaggtccag
    15121 ttggattggc tgctctttta acagcccaat tctattcacc agctaaattg attatggtag
    15181 acctagacga taaccgcttg gaaactgcag tatcctttgg tgctactcat aaggtaaact
    15241 cttcagaccc tgaaaaagcc attaaagaaa tttatgattt gacagatggt cgtggtgtgg
    15301 atgtcgctat cgaagctgtt ggtattcctg caacatttga tttctgtcaa aagattatcg
    15361 gtgtagacgg aacggttgcc aactgtggtg tgcatggtaa accagttgaa ttcgatttag
    15421 ataaactttg gattcgcaac atcaatgtaa caactggttt ggtatctaca aatacgactc
    15481 cacaattgtt gaaagcactt gaaagtcata agattgaacc agaaaaattg gtaactcact
    15541 atttcaaact tagtgaaatt gaaaaagcct acgaagtctt cagtaaggca gcagaccacc
    15601 atgccattaa ggtcattatc gaaaacgata tctcagaagc ctaagtagta aaaatatttt
    15661 tgtacataag caaatagaaa ttcagtcatc catcagatgg ctggattttt tatcaaaaaa
    15721 ttaagaaatg agcatatttc tttccttgtc tggcggaatt ggttataata tacggtacaa
    15781 aggaatgaat gaatatgtat cgtgttatag aaatgtacgg agattttgaa ccgtggtggt
    15841 tcttagaagg ttgggaagaa gatattgtag caagtagaaa atttgaccag tattatgatg
    15901 ctctcaaata ctacaaaact tgctggttta gattggaaca agaatcgcct ctttataaaa
    15961 gtagaagcga cttgatgacc attttttggg acccggaaga ccaacgctgg tgtgatgaat
    16021 gtgatgagta tttacaacaa taccattctt tggctctttt gcaggatgag caggttatcc
    16081 cagacgaaaa actacgctca ggctatgaaa aacaaaccag tcaggaaagg aatcgttctt
    16141 gccgtatgaa attaaaatag agaaaagtaa cttttttgga gttgcttttt ttatttttct
    16201 aactctttgc gaatagtata ggtgaggagg taagtatggt tcaagaaatt gcacaagaaa
    16261 tcattcgttc agctcggaaa aaagggacgc aggatatcta ttttgtccct aagttagacg
    16321 cctatgagct tcatatgagg gtaggagacg agcgctgtaa aattggtagc tatgattttg
    16381 aaaagtttgc agccgttatc agtcacttta agtttgtggc gggtatgaat gtgggagaaa
    16441 aaagacgtag tcaactgggt tcctgtgatt atgcctatga ccagaagata gcgtctctac
    16501 gtttatctac tgtaggcgat tatcgggggc atgagagttt ggttatccgt ttgttgcacg
    16561 atgaggagca ggacctgcat ttttggtttc aggatattga agaattaggc aagcagtaca
    16621 ggcaacgggg actctatctt tttgctggtc cggttgggag tggtaagacg accttgatgc
    16681 atgaattgtc caagtcactc tttaaaggac agcaagttat gtccatcgaa gatcctgtcg
    16741 aaatcaagca ggacgacatg cttcagttgc agttgaacga agcaatcggc ctaacctatg
    16801 aaaatctaat caaactttcc ttgcgtcatc gaccagatct cttgattatc ggagaaattc
    16861 gtgacagcga gacggcgcgt gcagtggtca gagctagttt gacaggtgcg atagtctttt
    16921 caaccattca cgccaagagt atccgaggtg tttatgagcg tctgctggag ttgggtgtga
    16981 gtgaagaaga attggcagtt gttttgcaag gagtctgcta ccagagatta atcgggggag
    17041 gaggaatcgt tgactttgca aacagagatt atcaagaaca ccaagcagcc aagtggaatg
    17101 agcaaattga ccagcttctt aaagatggac atatcacaag tcttcaggct gagacggaaa
    17161 aaattagcta cagctaagca aaaaaatatc atcaccctat ttaacaatct cttttctagc
    17221 ggttttcatc tggtggagac tatctctttt ttagatagga gtgctttgtt ggacaagcag
    17281 tgtgtgaccc agatgcgtgt gggcttgtct caggggaaat cattctcaga aatgatggaa
    17341 agtttgggat gttcaagtgc tattgtcact cagttatccc tagctgaagt tcatggcaat
    17401 ctccacctga gtttgggaaa gatagaagaa tatctggaca atctggccaa ggtcaagaaa
    17461 aaattgattg aagtagcgac ctatcccttg attttgctgg gttttcttct cttaattatg
    17521 ctggggctac ggaattacct gctcccacaa ctggatagta gcaatattgc cacccaaatt
    17581 atcggtaatc tgccacaaat ctttctagga atgatagttt ttgtttcagt aggtgtcctt
    17641 ttagcactca ctttttataa aagaagttct aagatgcgcg tcttttctat cttagcgcgc
    17701 cttcctttta ttggaatctt tgtgcagacc tatttgacag cctattatgc acgtgaatgg
    17761 gggaatatga tttcacaggg aatggagttg acgcagattt ttcaaatgat gcaggaacaa
    17821 ggttcccagc tctttaaaga aatcggtcaa gatctggctc aaaccctgaa aaatggccgt
    17881 gaattttctc agacgatagg aacctatcct ttctttagga aggaattgag tctcatcata
    17941 gagtatgggg aagttaagtc caagctgggt agtgagttgg aaatctatgc tgaaaaaact
    18001 tgggaagcct tttttacccg agtcaaccgc accatgaatt tggtgcagcc actggttttt
    18061 atctttgtgg cactgattat cgttttactt tatgcggcaa tgctcaggcc catgtatcaa
    18121 aatatggagg taaattttta aaatgaaaaa aatgatgaca ttcttgaaaa aagctaaggt
    18181 taaagctttt acattggtgg aaatgttggt ggtcttgctg attatcagcg tgcttttctt
    18241 gctctttgta cctaatctga ccaagcaaaa agaagcagtc aatgacaaag gaaaagcagc
    18301 tgttgttaag gtggtggaaa gccaggcaga actttatagc ttggaaaaga atgaagatgc
    18361 tagcctaagc aagttaaaag acgatgggcg aatcacggaa gaacaggtta aagcttataa
    18421 agaatacaat gataaaaatg gaggagcaaa tcgtaaagtc aatgattaag gcctttacca
    18481 tgctggaaag tctcttggtt ttgggtcttg tgagtatcct tgccttgggc ttgtccggct
    18541 ctgttcagtc cacttttgcg gcagtagagg aacagatttt ctttatggaa tttgaagaac
    18601 tctatcggga aacccaaaaa cgcagtgtag ccagtcagca aaagactagt ctgaacttag
    18661 atgggcagac gattagcaat ggcagtcaaa agttgccagt ccctaaagga attcaggtcc
    18721 catcaggcca aagtattaca tttgaccgtg ctgggggcaa ttcgtccctg gctaaggttg
    18781 aatttcagac cagtaaagga gcgattcgct atcaattata tctaggaaat ggaaaaatta
    18841 aacgcattaa ggaaacaaaa aattagggca gtgattttac tggaagcagt agtcgctcta
    18901 gctatctttg ccagcattgc gaccctcctt ttgggacaaa ttcaaaaaaa taggcaagag
    18961 gaagcaaaaa tcttgcaaaa ggaagaagtc ttgagggtag ctaagatggc cttgcagaca
    19021 ggtcaaaatc aggtaaacat aaacggagtt gagattcagg tgttttctag cgaaaaggga
    19081 ttggaggtct accatggttc agaacagttg ttggcaatca aagagccata aggtcaaggc
    19141 ttttaccttg ttagaatccc tgcttgccct gcttgtcatc agtgggggct tactcctttt
    19201 tcaagctatg agtcagctcc tcatttcaga agttcgctac cagcaacaaa gcgagcaaaa
    19261 ggagtggatt ttgtttgtgg accaacttga ggcagaatta gaccgttcgc agttcgaaaa
    19321 agtagaaggc aatcgcctat acatgaagca agatggcaag gacatcgcca tcggtaagtc
    19381 aaagtcagac gattttcgta aaaccgatgc tagtggacgg gggtatcagc ctatggttta
    19441 tggcctcaaa tcagctcaga ttacagagga caatcaactg gttcattttc gtttccagtt
    19501 tcaaaaaggc ttagaaaggg agttcatcta tcgtgtggaa aaagaagaaa gttaaggcag
    19561 gtgttctcct ctacgcagtc accatagcag ccatctttag tcttttgttg caattttatt
    19621 tgaaccgaca aatcgcccac tatcaagact atgctttaaa taaagaaaaa ttggttgctt
    19681 ttgccatggc taaacgaacc aaggataaag ccgagcaaga aagtggggaa caggttttta
    19741 atctaggtca ggtaagctat caaaacaaga aaacaagctt ggtgacgact gttcgtacgt
    19801 ctaagagcca atatgagttt ctgtttcctt cagtcaaaat caaagaagag aaaagagata
    19861 aaaaggaaga ggtagcgacc gattcaagcg aaaaagcgga gaaaaaaaat cagaagagaa
    19921 gcctgaaaag aaagagaatt cctagtcaat tcaactataa tgcgttgaat ccagaatagt
    19981 ccactgtagt ttctagaaaa ttgctggaaa tggatgttaa gctccaattc atttgtttat
    20041 atcttatttc agtttactat actttatgct aaattaaaga tatgaaacat gattttaacc
    20101 acaaagcaga aactttcgat tcccctaaaa atatcttcct cgcaaacttg gtatgtcaag
    20161 cagtcgagaa acagattgat attttatcag acaaagaaat tttagatttc ggtggtggca
    20221 cgggtctatt agtcttgccc ctagccaagc aggctaagtc agtcactctt gtagacattt
    20281 ctgagaaaat gttggagcaa gctcgtttga aagtggagca gcaagcaatc aagaatatcc
    20341 agtttttgga gcaagattta ccgaaaaatc ccttggagaa agagtttgat tgccttgctg
    20401 ttagtcgggt tcttcatcat atgcctgatt tggatgcggc tctctcactg tttcatcaac
    20461 atttgaagga agatgggaaa ctcatcattg ctgattttac caagacagaa gctaatcatc
    20521 atggatttga tttagctgaa ctggaaaaca agctaattga gcatggtttt tcatctgtgc
    20581 atagtcagat tctctatagc gctgaagacc tgtttcaagg aaatcactca gaattctttt
    20641 taacagtagc ccaaaaatca ctcgcctagt cagggagtga tttttctata aggatggaaa
    20701 aaagaaggga aatttggtaa gataggaata tggattttga aaaaattgaa caagcctata
    20761 cctatttact agagaatgtc caagtcatcc aaagtgattt ggcgaccaac ttttatgacg
    20821 ccttggtgga gcaaaacagc atctatctgg atggtgaaac tgagctaaac caggtcaaag
    20881 acaacaatca ggcccttaag cgtttagcac tacgcaaaga agaatggctc aagacctacc
    20941 agtttctctt gatgaaggct gggcaaacag aacccttgca ggccaatcac cagtttacac
    21001 cagatgccat tgctttgctt ttggtgttta ttgtggaaga gttgtttaaa gaggaggaaa
    21061 ttactatcct cgaaatgggg tctgggatgg gaattctagg cgctactttc ttgacctcgc
    21121 ttgataaaaa ggtggattac ttgggaatgg aaatggatga tttgctgatt gatctggcag
    21181 ctagcatggc agatgtaatt ggtttgcagg ctggctttgt ccaaggagat gccgttcgtc
    21241 cacaaatgct caaagaaagc gatgtggtca tcagtgactt gcctgttggc tattatcctg
    21301 atgatgccgt tgcgtcgcgc catcaagttg cttctagcca agaacatact tacgcccatc
    21361 acttgctcat ggaacaaggg cttaagtacc tcaagtcaga cggatacgct atttttctag
    21421 ctccgagtga tttgttgacc agtcctcaaa gtgatttgtt aaaagaatgg ctgaaagaag
    21481 aggcgagtct ggttgctatg attagtctgc ctgaaaatct ctttgctaat accaaacaat
    21541 ctaagactat ttttatctta cagaagaaaa atgaaatagc agtagatcct tttgtttatc
    21601 cacttgctag cttgcaagat gcaagtgttt taatgaaatt taaagaaaat tttcaaaaat
    21661 ggactcaagg tactgaaata taaaatagat tttgttataa tagttgaaaa cgcttaaaaa
    21721 ggggtatcat gttatgacaa aaacaattgc aatcaatgca ggaagttcaa gtttgaaatg
    21781 gcaattatac ttaatgccag aagaaaaagt attggcgaaa ggtttgattg aacgtatcgg
    21841 tttgaaagat tcaatttcaa ctgtaaaatt tgacggccgt tctgagcaac aaattttgga
    21901 tattgaaaat catacacaag ccgttaaaat tttattggat gacttgattc gtttcgatat
    21961 tatcaaggct tatgacgaga ttacaggtgt tggacatcgt gttgttgctg gtggagaata
    22021 tttcaaagaa tcaacagttg ttgagggaga tgttttagaa aaagttgaag agttgagttt
    22081 gttggctcct ctacacaacc cggccaatgc agcaggtgtt cgtgccttca aggaattgtt
    22141 gccagacatt accagtgtag ttgtttttga tacttccttc cacacaagta tgccagagaa
    22201 agcttatcgc taccctctac caacaaaata ttacacagaa aacaaggttc gtaaatacgg
    22261 tgctcatggt acaagtcacc agtttgtagc aggagaggct gcaaaactct tgggacgtcc
    22321 attagaagac ttgaagttaa ttacctgtca tattggtaac ggaggctcaa ttacagctgt
    22381 gaaagccggc aaatctgtag acacttctat ggggttcact cctcttggtg gtattatgat
    22441 gggaacgcgt acaggggata ttgatccagc tatcattcct tatttaatgc aatatacaga
    22501 ggattttaac acaccagaag atatcagtcg tgttcttaac cgtgaatcag gtcttttggg
    22561 agtttctgct aattctagcg atatgcgcga tatagaagca gctgtagcag aagggaatca
    22621 cgaggctagc ttggcttatg aaatgtatgt tgaccgtatc caaaaatata tcggtcagta
    22681 ccttgcagtg ctaaatggag cagatgccat tgttttcaca gcaggtgtcg gtgaaaatgc
    22741 agagaatttc cgtcgtgatg taatctcagg gatttcgtgg tttggttgtg atgttgatga
    22801 tgaaaagaat gtctttggcg ttacaggaga catctcaaca gaggcagcta aaatccgtgt
    22861 cttggttatt ccaacagatg aagaattagt cattgcccgt gacgttgaac gcttgaaaaa
    22921 ataagtgaaa ctaaaaaaat attcaataca aggagttggg aaagttattt ttccagcttc
    22981 tttttctgat gaaattgtcc aaaaccttgc tatgattggc ttttttgaaa aatatggtat
    23041 aatagtagta atttaataga tggagttgag ttttgaagaa aaactttcgt gtaaaaagag
    23101 agaaagattt taaggcgatt ttcaaggagg ggacaagttt tgctaatcgc aaatttgtgg
    23161 tctaccaatt agaaaaccag aaaaaccatt ttcgagtagg tctatcagtt agcaaaaaac
    23221 tggggaatgc cgtcactaga aatcaaatta agcgacggat tcggcatatt atccagaatg
    23281 caaaagggag tctggtagaa gatgtcgact ttgttgtcat tgctcgaaaa ggagtcgaaa
    23341 ccttgggata cgcagagatg gagaaaaatc tactccatgt attaaaatta tcaaagattt
    23401 accgggaagg aaatgggagt gaaaaagaaa ctaaagttga ctagtttgct aggactgtct
    23461 ctgttaatca tgacagcctg tgcgactaat ggggtaacta gcgatattac agccgaatcg
    23521 gctgattttt ggagtaaatt ggtttacttc tttgcggaaa tcattcgctt tttatcgttt
    23581 gatattagta tcggagtggg gattattctc tttacggtct tgattcgtac agtcctcttg
    23641 ccagtctttc aggtgcaaat ggtggcttct aggaaaatgc aggaagctca gccacgcatt
    23701 aaggcgcttc gagaacaata ttcaggtcga gatatggaaa gcagaaccaa actagagcag
    23761 gaaatgcgta aagtatttaa agaaatgggt gtcagacagt cagactctct ttggccgatt
    23821 ttgattcaga tgccggttat tttggccctg ttccaagccc tatcaagagt tgacttttta
    23881 aagacaggtc atttcttatg gattaacctt ggtagtgtgg atacaaccct tgttcttccg
    23941 attttagcag cagtattcac ctttttaagt acttggttgt ccaacaaagc tttgtctgag
    24001 cgaaatggcg ctacgactgc gatgatgtat gggattccag tcttgatttt tatctttgca
    24061 gtttatgcgc caggtggagt cgccctatac tggacagtgt ctaatgctta tcaagtcttg
    24121 caaacctatt tcttgaataa tccattcaag attatcgcag agcgcgaggc cgtagtacag
    24181 gcacaaaaag atttggaaaa tagaaaaaga aaagccaaga aaaaggctca gaaaacgaaa
    24241 taaataagga ggaatctggt aatggtagta tttacaggtt caactgttga agaagcaatc
    24301 cagaaaggat tgaaagaatt agatattcca agaatgaagg ctcatatcaa agtcatttct
    24361 agggagaaaa aaggctttct tggtctattt ggtaaaaaac cagcccaagt ggatattgaa
    24421 gcgattagtg aaacgactgt tgtcaaagca aatcaacagg tagtaaaagg cgttccgaaa
    24481 aaaatcaatg atttgaacga gcctgtgaag acggttagtg aagaaaccgt tgaccttggt
    24541 catgtggttg atgctattaa aaaaatagag gaagaaggtc aaggtatttc tgatgaagtc
    24601 aaggctgaaa tcttaaaaca tgaaagatat gccagcacta tcttagaaga aactggtcac
    24661 attgagattt taaatgaact tcaaatcgag gaagcgatga gggaagaagc aggcgctgat
    24721 gaccttgaaa ctgagcaaga tcaaactgaa aatcaagact tgaaagagat gggcttgaag
    24781 gtcgagcaaa gttatgatat tgcccaggtg gctacggatg tgactgctta tgttcaaacg
    24841 attattgatg acatggatgt tgaggctaca ctttcaaatg attataaccg tcgtagcatc
    24901 aatttgcaaa ttgatactaa cgaaccaggt cgtattatcg gctaccatgg taaagtcttg
    24961 aaggccttgc aactgttggc tcaaaattat ctttacaact gctattcaag aaccttctat
    25021 atcactatta atgtcaatga ttatgttgaa caccgtgcag aagtcttgca gacctatgcg
    25081 caaaaattgg cgattcgtgt tttagaagaa ggacgtagtc atcaaacaga tccaatgtcg
    25141 aatagtgaac gcaagattat ccaccgtatt atttcacgta tggatggcgt gaccagttac
    25201 tctgaaggtg atgagccaaa tcgctatgtc gttgtagata cagaataagt aaaatcaggg
    25261 ttgacctgat tttttgctag ctagaggagg ttaaactgat gttgaataag ataagagact
    25321 atctagactt ttctggtttg cagtaccgta gtcctgataa agcgggagca gagcgagaga
    25381 agatgctggc gttccgccac aaaggacaag aggcccgaaa ggcttttaca gaactggcca
    25441 aagcctttca agcaagccat ccagaatggc aactccaaca gactagtcag tggatgaatc
    25501 aggcccagcg tttgagacca catttttggg tttatctaca gagagacgga caagtgacag
    25561 aacctatgat ggccttacgt ttgtatggga catctactga ctttggaatt tctttggaag
    25621 tcagtttcat cgaacgtaag aaggatgagc aaacactggg caagcaggcc aaagttttag
    25681 acattccaac cgttaaaggg atttattatc taacctactc taatggtcaa agtcaacggt
    25741 gggaggcgaa tgaagaaaag cgtcgtactt tacgcgagaa ggtgagaagt caagaagttc
    25801 gaaaagtttt agtgaaggta gatgttccta tgacagaaaa ttcgtctgaa gaagaaatcg
    25861 tagaaggctt attgaagtct tattctaaaa ttcttcccta ttatctagct acgagaaaat
    25921 aagataattt gtaaaacatc ataaatcata cagtccaaga gtgaacagtc cgctgtgtaa
    25981 ttcttggtct ttttgtttgc gctttcgcat tatataataa acttacaaaa acaattcaaa
    26041 aggagaacaa ttatggaagt cgtttcaagt gttctaaatt ggttttctag caatattttg
    26101 cagaatcccg catttttcgt aggtttattg gtgttgatag gatatgcact tttgaaaaaa
    26161 cctgcccatg acgttttttc agggtttgtt aaagcaacag tagggtatat gttgcttaac
    26221 gtgggtgctg gtggtttggt tacaaccttt cgtccaatct tagcagctct taactacaaa
    26281 ttccaaattg gtgcagcggt tatcgaccct tattttggac ttgctgcagc aaacaacaaa
    26341 attgtagcag agtttccaga ttttgttgga actgcaacta cagctctatt gattggtttt
    26401 ggaataaata tcttgctcgt agctcttcga aagattacga aggtaagaac cctctttatt
    26461 actggtcaca tcatggtaca acaagctgca acagtatctc ttatggttct attcttagta
    26521 ccacaattgc gcaatgctta cggtacagca gcgattggta tcatctgtgg actttactgg
    26581 gcagttagtt caaatatgac tgttgaggca actcaacgct tgactggtgg tggcggattt
    26641 gcgattggtc accaacagca atttgcaatc tggtttgtag ataaagtagc aggacgcttt
    26701 ggtaagaaag aagaaagttt agacaatctt aaattaccta agttcctctc aatcttccac
    26761 gatacagttg ttgcatctgc taccttgatg ctcgtattct tcggagccat tcttttaatc
    26821 ttgggtccag acattatgtc taataaagaa gtcatcactt caggaactct attcaatcct
    26881 gctaaacaag atttctttat gtacattatc caaacagcct ttaccttctc agtttacttg
    26941 ttcgttttga tgcaaggtgt ccgaatgttc gtatctgagt tgacaaacgc cttccaaggt
    27001 atttcaaaca aattgttgcc aggttcattc ccagcggttg acgttgcagc ttcttatgga
    27061 tttggttctc caaatgctgt cttgtcagga tttacctttg gtttgattgg tcaattgatt
    27121 acaattgttt tgctcatcgt ctttaaaaat ccgattctta ttattacagg atttgtacca
    27181 gtgttctttg acaatgcagc cattgcggtc tacgctgata aacgcggcgg atggaaagcg
    27241 gctgttatcc tttcctttat atcaggtgtc cttcaagttg ctctaggagc tctttgtgtg
    27301 gcccttctcg atttggcatc ttatggtggc taccatggaa atatcgactt tgaattccca
    27361 tggcttggat ttggatatat cttcaaatac cttggtattg ttggttatgt acttgtgtgt
    27421 ctcttcttgc ttgttattcc tcaacttcaa tttgccaaag caaaagataa agagaaatat
    27481 tacaacggtg aagttcaaga agaagcttag tatctagaaa aggagaaata aaatggttaa
    27541 agtattagca gcgtgcggaa atggaatggg ttcatcaatg gttatcaaga tgaaggttga
    27601 aaatgctctc cgtaagctta atcaaacaga ttttacagtc aattcatgca gtgtcggtga
    27661 agctaaaggt ttagcagtag gatatgacat tgtaatcgct tctcttcatt tgattcaaga
    27721 attggaaggg cgaactaatg ggaagttaat tgggcttgat aacttgatgg atgataaaga
    27781 aatcaccgaa aaactcagtc aagcactaca gtaaaaggtt ggagggggct ggacagaaac
    27841 tgagagttat cgtttctgtc cagccccctc tttaaataaa ggaggcagat atgaatttaa
    27901 aacaagcttt aattgacaat gactcgatcc gactaggttt agaggctaac gattggaaag
    27961 aagcagtcaa ggtagcagta gatcccttga ttgaaagtgg ggcaattttg ccagagtatt
    28021 acgatgctat cattgaatcg actgaagagt atgggcctta ctatatcttg atgccaggta
    28081 tggctatgcc ccacgctaga cctgaagctg gtgtgcaaag tgatgccttt tcattgatta
    28141 ccttacaaaa tcctgttgta ttttcagatg ggaaagaggt atctgttttg ttggcactag
    28201 cagcaacaag ttcaaaaatt cacacaagtg tagccattcc acaaattatt gccctgtttg
    28261 aattagaaga ttctattgca cgtttacagg cttgccagac taaagaagat gtcttggcta
    28321 tgattgaaga atctaaggat agcccttatc tcgaaggatt ggatttggaa agttagaaag
    28381 aggaataaag aaatgacaaa aagaatacct aatttacaag ttgcattaga ccattcagac
    28441 ttgcaaggag cgattaaagc agctgtttct gttggtcagg aagtagatat tatcgaagct
    28501 ggaactgttt gcttgcttca agttggaagt gaactggctg aagtcttgcg tagccttttc
    28561 ccagataaga ttattgtggc agacacaaaa tgtgctgatg ctggtggaac agttgctaaa
    28621 aataatgcgg ttcgtggagc agactggatg acttgtatct gttgtgcaac catccctact
    28681 atggaagcag ctctaaaggc tatcaagact gaacgaggag aacgaggcga aatccagatc
    28741 gagctttatg gcgattggac ttttgaacaa gctcagcttt ggctagatgc aggtatctca
    28801 caagctattt atcaccaatc tcgtgatgct cttcttgctg gtgaaacttg gggtgaaaaa
    28861 gaccttaata aggttaaaaa actcattgac atgggcttcc gtgtatctgt aacaggtggt
    28921 ctagatgtag atactctcaa actctttgaa ggtgttgatg tctttacctt tatcgcaggt
    28981 cgtggaatta cagaggctgc ggatccagca ggagcagcgc gtgccttcaa ggatgaaatc
    29041 aaacgaattt gggggtaaat catggtacgt ccaattggaa tttatgaaaa ggcaacccca
    29101 acacacttta cttggctaga acgtttaaat tttgccaagg agttaggctt tgattttgtc
    29161 gagatgtcta ttgacgaacg tgacgagcgt ttagcaagac ttgactggag taaggaagaa
    29221 cgcttggaag ttgtcaaagc aatctatgaa actggtgttc gtattccttc tatctgtttt
    29281 tcaggccatc gtcgctaccc attgggttca aaagatccag ttctagagga aaaatctcta
    29341 gaactcatga aaaaatgtat cgaattagct caagacttgg gagttcgtac gattcaatta
    29401 gctggttacg atgtttacta tgaggaaaag tcaccccaga cacgccaacg ttttatcaaa
    29461 aatttgagaa aagcctgtga ctgggctgaa gaagctcagg tggtacttgc tattgaaatt
    29521 atggatgatc ctttcatcag tagcatcgaa aaatatttgg ctatagaaaa agagattgac
    29581 tctcccttcc tctttgtata tccagatatt ggtaatgtgt ctgcatggca taatgatatc
    29641 tatagtgagt tttatcttgg tcatcatgcc atcgcagctc tccatctcaa ggatacttat
    29701 gcagtgacag aaagttcaaa gggccagttc cgagatgtac ctttcgggca aggttgtgtt
    29761 aaatgggaag aagctttcga tattttaaag gaaaccaatt ataatggacc tttcctaatc
    29821 gaaatgtggt ctgaaaattg tgaaacagta gaagaaacac gcgcagccgt tcaagaggcg
    29881 caagcttttc tctatccact cattaagaaa gcaggtttga tgtaagatga atcaagtaat
    29941 caatgctatg cgtaaacgag tctgtgatgc caatcaatca ttgccaaaac atggacttgt
    30001 caaatttacc tgggggaatg tatctgaagt taatcgcgaa ctcggtgtca ttgttatcaa
    30061 accatcaggc gtggattatg acgaattgac acctgaaaac atggtagtga ctgatctaga
    30121 tggtaagatc ctagaagggg atttaagacc atcttccgac ctcccaactc atgtgcaatt
    30181 atataaggct tggtcagaaa ttggtagtgt ggttcacacc cattcgacag aagctgttgg
    30241 ttgggctcag gcaggtcgtg atattccttt ctacggaaca acccatgcag attatttcta
    30301 cggttcaatc ccttgcgccc gtagtttgac caaggacgaa gtagaagtgg cctatgaaaa
    30361 agatactggc ctggttatcg tagaagagtt tgaacatcgc ggacttaacc cggttgaagt
    30421 accaggaatt gttgtacgca atcacggtcc attcacctgg ggcaaaaatc cagagaatgc
    30481 tgtttatcac tctgtcgtac tagaggaagt atcaaagatg aatcgattta cagaacaaat
    30541 caatccaaga gttggacctg ctccccagta catactagaa aaacactacc aacgtaaaca
    30601 tggaccaaat gcttattatg gtcaaaagta agaacgatga aggaggagaa aaagataaat
    30661 ttagctcctc tttttacatt tgatttttat tgagagtaaa gttggaattg aagtaatttt
    30721 aaaagatttt ttagaaatag cgcttgatat atatatatgg taaaataaaa agaattgctg
    30781 tgatatcaat agatttgggg gattttttaa tatggtactg gataaggcaa gttgtgattt
    30841 gcttcaatat ttgatggatc aagaaacgtc caaaacgatt atggcgattt cgaaagattt
    30901 gaaagagtca agaaggaaaa tttattatca cattgacaaa atcaatgctg ctctggttga
    30961 cgaggcgctt cacatcatta gtattccacg aattggtatt cacttaacgg aagagcagag
    31021 agatgcttgt tgtaaactat tatcggaagt agattcgtac gattatatca tgagtgcgca
    31081 tgaacgtatg atgataatgt tactatggat aggtatttct aaagaacgta ttacgattga
    31141 aaaattgata gagttaacag aggtatctag gaatactgtt ctcaatgatt tgaatagtat
    31201 tcgttatcaa ctaactttgg aacaatatca ggtgatcttg caagtgagca agtcacaggg
    31261 ataccacctt catgcccacc ctcttaataa aattcagtat cttcaatcgc ttctatatca
    31321 tatttttatg gaagaaaatg ccacttttgt atctatttta gaagataaga tgaaagagag
    31381 gttagatgat gagtgtttgc tttctgttga aatgaaccaa ttttttaagg aacaggttcc
    31441 tttagttgaa caagatttag ggaagaaaat aaaccatcat gaaataactt ttatgttgca
    31501 ggttctacct tatttgctgt taagctgtca taatgttgaa cagtatcaag aaagacatca
    31561 ggatatagag aaagaatttt ctttgataag aaaaagaata gagtatcagg tgtctaagaa
    31621 attaggagaa cggttgtttc aaaagtttga aatttctttg tcaggacttg aagtttctct
    31681 tgtagctgtt ctcctcctct cctatcgtaa agatttggat attcatgcag aaagtgatga
    31741 ttttcggcaa ttaaaacttg ctttagaaga atttatctgg tattttgaat cacaaatccg
    31801 aatggagatt gagaacaagg atgatttgtt acgaaatttg atgatccact gtaaagcctt
    31861 gttatttaga aagacttacg gtattttttc taaaaatcct ctaacaaaac aaattcgatc
    31921 caagtatgga gaattatttt tagtcactag aaaatctgcg gaaattttag aaggagcatg
    31981 gtttattcgg ctaacagacg atgatattgc ctatttgacg attcatattg gaggattttt
    32041 aaagtataca ccatcgtctc aaaaaaatat gaaaaaagtt tatctcgttt gtgatgaagg
    32101 tgttgcggtt tcgagacttt tgctgaaaca atgcaaactt tattttccaa atgagcaaat
    32161 tgacactgta tttacaacag aacaatttaa gagtgtggaa gatattgcac aagttgatgt
    32221 agtgattact actaatgatg atttggatag cagatttccg attttaaggg ttaatcctat
    32281 ccttgaagca gaagatattt tgaaaatgct agactatctt aaacacaata tatttcgtaa
    32341 tgagagcaaa agtttcagtg aaaatctttc tagtcttatt tcgtcttata ttgtagatag
    32401 caagttggct agtaagttcc aagaagaggt tcaaacactt ataaatcaag aaatagtagt
    32461 tcaagctttt ttggaagata tttgaaggac agtccaatga tgaacacaaa cctgtgtttt
    32521 tcttggtctt ttttagtgtt ttgaagggtg gtatactaat ctcaaagata acaattatat
    32581 ccaaaggagg caacatatgc caaacgtcaa agaaattaca agagagtcat ggattttagc
    32641 cactttccca gagtggggaa catggttgaa cgaagaaatc gaagaagaag tcgtacctga
    32701 aggcaacttt gccatgtggt ggctaggcaa ctgtggtact tggattaaga caccagctgg
    32761 tgctaacgtt gtcatggacc tttggtcaaa ccgtggaaaa tcaaccaaaa aagtgaaaga
    32821 tatggttcgt gggcaccaaa tggcaaatat ggcaggtgtt cgtaagttgc aaccaaactt
    32881 gcgtgttcag ccaatggtta tcgatccatt tgctatcaac gaactagact attacttagt
    32941 ttcacacttc cacagtgatc atatcgaccc atacacagct gcagcaattc tcaacaatcc
    33001 taagttagag catgttaagt ttatcggtcc ttaccactgt ggacgaatct gggaaggatg
    33061 gggtgttcca aaagaacgta tcatcgttgt taaaccaggt gacactatcg aattaaaaga
    33121 tatgaagatt catgcagtag aatcatttga ccgtacttgc ttggtaactc tcccagtgaa
    33181 cggtgctgat gagacaggcg gtgaacttgc tggcttggct gttacagatg aagaaatggc
    33241 tcaaaaggct gttaactata tctttgaaac accaggtgga accatctatc atggtgcaga
    33301 ttctcacttc tcaaactatt ttgcaaaaca tggtaaagac tttaaaattg atgttgcttt
    33361 gaataactat ggtgaaaatc cggtaggtat ccaagacaaa atgacatcta tcgaccttct
    33421 tcgtatggca gaaaatctgc gtaccaaagt cattatccca gttcactatg atatctggtc
    33481 taacttcatg gcttctacta atgagattct agaactttgg aaaatgcgaa aagatcgctt
    33541 gcaatacgat ttccatccat ttatctggga agttggcggt aagtacactt atcctcaaga
    33601 tcaacactta gtagaatacc atcatccacg tggttttgat gattgttttg aacaagactc
    33661 taacattcaa tttaaagctt tgctataata taaaaatatt ctttggaggt tatccgtatg
    33721 ttgcatgata cggataattt tcatataata gtaaaataga atgtgtgatt caataatcac
    33781 ctcaaataga aaggaaattc tatgtcaaat ctatctgtta atgcaattcg ttttctaggt
    33841 attgacgcca ttaataaagc caactcaggt catccaggtg tggttatggg agcggctccg
    33901 atggcttaca gcctctttac aaaacaactt catatcaatc cagctcaacc aaactggatt
    33961 aaccgcgacc gctttattct ttcagcaggt catggttcaa tgctccttta tgctcttctt
    34021 cacctttctg gttttgaaga tgtcagcatg gatgagatta agagtttccg tcaatggggt
    34081 tcaaaaacac caggtcaccc agaatttggt catacggcag ggattgatgc tacgacaggt
    34141 cctctagggc aagggatttc aactgctact ggttttgccc aagcagaacg tttcttggca
    34201 gccaaatata accgtgaagg ctacaatatc tttgaccact atacttacgt tatctgtgga
    34261 gacggagact tgatggaagg tgtctcaagc gaggcagctt catacgcagg tttgcaaaaa
    34321 cttgataagt tggttgttct ttatgattca aatgatatca acttggatgg tgagacaaag
    34381 gattccttta cagaaagtgt tcgtgaccgt tacaatgcct acggttggca tactgccttg
    34441 gttgaaaatg gaacagactt ggaagccatc catgctgcta tcgaaacagc aaaagcttca
    34501 ggcaagccat ctttgattga agtgaagacg gttattggat acggttctcc aaacaaacaa
    34561 ggaactaatg ctgtacacgg cgcccctctt ggagcagatg aaactgcatc aactcgtcaa
    34621 gccctcggtt gggactacga accatttgaa attccagaac aagtatatgc tgatttcaaa
    34681 gaacatgttg cagaccgtgg cgcatcagct tatcaagctt ggactaaatt agttgcagat
    34741 tataaagaag ctcatccaga actggctgca gaagtagaag ccatcatcga cggacgtgat
    34801 ccagtcgaag tgactccagc agacttccca gctttagaaa atggtttttc tcaagcaact
    34861 cgtaattcaa gtcaagatgc cttgaatgtt gtggcagcta aattaccaac cttcctaggt
    34921 ggatcagctg accttgctca ctcaaacatg acttatatca agacggatgg acttcaagac
    34981 gataccaatc gcttgaatcg taacattcag tttggtgttc gtgaatttgc aatgggaaca
    35041 atcttgaacg ggatggccct tcatggtgga cttcgtgtat acggtggaac tttcttcgtc
    35101 ttctctgact atgtgaaggc agctgtccgc ttgtcagcct tacaaggact tcctgtgact
    35161 tatgtcttta cccatgattc aatcgcagtt ggggaagatg gtccgactca cgaaccagtt
    35221 gagcatttag caggtcttcg tgctatgcca aatctaaatg ttttccgtcc agcagatgcg
    35281 cgtgaaacgc aagcagcttg gtaccttgca gtgacaagtg agaaaacacc aactgccctt
    35341 gtcttgacac gtcaaaactt gactgttgaa gatggaacag acttcgacaa ggttgctaaa
    35401 ggtgcttatg ttgtatatga aaatgcagcc gactttgata ccatcttgat tgcgacaggt
    35461 tcagaggtta atcttgctgt ctcagctgct aaagaattgg ctagtcaagg cgaaaaaatc
    35521 cgcgtagtca gcatgccatc tacagatgtc tttgataaac aagatgcagc ttacaaggaa
    35581 gagattcttc caaatgcagt ccgccgtcgt gttgcagtcg aaatgggtgc aagtcaaaac
    35641 tggtacaaat atgttggtct cgatggtgcc gttctaggta ttgatacctt cggagcctct
    35701 gccccagcac caaaagtatt ggcagaatat ggctttactg tagaaaatct tgtaaaagtt
    35761 gttcgaaact tgaaataatc ctaaaaatca gggcgtaagc tctggttttt cttaccagaa
    35821 aagtaaggta caatcttgta aaagtagctg aaatttgata tagtagtcct atgtaaaaga
    35881 caaaggagaa aacaatgaat ccaaatatta cttttttaat catgcttgta ggtatgatgg
    35941 ccttgatgtt ctttatgcaa cgttctcaaa agaaacaagc tcaaaagcgt atggaaagct
    36001 taaataaact acaaaaaggc tatgaagtga ttacaatcgg tggactttat ggaacagtcg
    36061 atgaagtaga tacggagaag ggaacaatag ttcttgacgt agatggagtt tacttgactt
    36121 ttgaactagc tgctatcaag acagtattac cgctgaaaga aacagcttca ctagaaggcg
    36181 caattgaaaa ataagacggg atcacgaact cccgtttttc tataaaagaa aggaaatggg
    36241 atgaaaaaat tagtctttgt ctgtctggga aatatttgcc gtagccctat ggccgagttt
    36301 gttatgaaat caatgacaga taactacgaa atccaaagtc gagcaacttc ctcttgggaa
    36361 catggcaatc cgattcataa ggggactcag ggaatttttc aagagtatga gattccttat
    36421 gacaagaaca agacatcgct tcagattagt aaggaagatt ttgaagcctt tgattatatt
    36481 atcggaatgg acgcttcaaa tatttctgac ttacgtcaga tgtgtccagt agactgtcaa
    36541 gataagattt actcattttc atctgaaagt gttccagacc cttggtatac aggagatttt
    36601 gaagaaacct atcgacgtgt tcaagagggc tgtcaagctt ggttagaacg tttagaaaag
    36661 gagagttaag gtggaaaatc ttacgaattt ttacgaaaag tatcgtgtct atctgactcg
    36721 tccacgatta gagcttttgg cagtagttac cattgttttc tgtgctgtac tcgtcttttt
    36781 tctaaatatt ccaggaaaag gtgtcttaaa actcgataat ggaacgattg tttatgatgg
    36841 cagtcttgtc cgtggtaaaa tgaatggtca aggtaccatt accttccaaa atggagacca
    36901 atatacaggt ggcttcaaca atggagcctt caacggaaaa ggtacctttc aatctaaaga
    36961 aggctggacc tacgaaggtg attttgtaaa tggtcaggct gaaggaaaag ggaaactaac
    37021 aacagaacaa gaagtcgttt atgaaggaac ttttaaacaa ggcgtttttc aacaaaaata
    37081 aagcctcctt atcaaaggag gtattattag aattacaagg taagcgctta cctgtaaatc
    37141 cctttctttc caaatccctc ttccaagcaa gtttgtgaaa taaaaaatat ttgaaataaa
    37201 tttcacaaac ttcaaagata aaacctgata agaaaagaaa atgagaaaag tttcacaaga
    37261 gtttaaaaat tctttgtgaa atgtttatga aactgtttac aaagttgaaa taatacgtta
    37321 taataaactt gtgaaaaaat taacaaaggg tatcgatcct tgaaagctat ggaggaaaat
    37381 atggctgata aaaaaactgt gacaccagag gaaaagaaac tcgttgctga aaaacacgta
    37441 gatgagttgg ttcaaaaagc tctagttgcc cttgaagaaa tgcgtaaatt ggatcaagag
    37501 caagttgact acatcgttgc caaagcatca gtagcagctt tggatgccca cggagaattg
    37561 gctttacatg cctttgaaga aacaggacgt ggtgtatttg aagataaagc aactaagaac
    37621 ttgtttgcct gtgaacacgt agtaaacaac atgcgtcaca ctaagacagt tggcgttatc
    37681 gaagaagacg atgtaacagg attgactctt attgctgaac cagttggtgt tgtttgtggt
    37741 attactccaa caacaaaccc aacatcaaca gcaatcttca aatcattgat ttcattgaag
    37801 acacgtaacc caatcgtctt tgccttccat ccatcagcac aagaatcatc tgctcatgca
    37861 gctcgtatcg tccgcgatgc agctatcgca gctggtgctc ctgaaaactg tgtgcaatgg
    37921 attactcaac catctatgga agcaacaagt gcccttatga accacgaagg tgttgcgaca
    37981 atccttgcaa caggtggtaa tgccatggtt aaggcggctt attcatgtgg taaaccagct
    38041 cttggggtag gtgccggaaa cgttccagct tatgttgaaa aatcagcaaa cattcgtcaa
    38101 gcagcacacg atatcgtcat gtctaaatca tttgataacg gtatggtctg tgcatctgaa
    38161 caagcagtta tcattgataa agaaatttac gatgaatttg tagcagagtt caaatcttac
    38221 cacacttact ttgtaaacaa aaaagaaaaa gctcttcttg aagagttctg cttcggcgtc
    38281 aaagcaaaca gcaaaaactg tgctggtgca aaattgaacg ctgacatcgt tggtaaacca
    38341 gcaacttgga ttgcagaaca agcaggattt acagttccag aaggaacaaa cattcttgct
    38401 gcagaatgta aagaagttgg cgaaaatgag ccattgactc gtgaaaaatt gtcaccagtt
    38461 attgcagttt tgaaatctga aagccgtgaa gatggtatta ctaaggctcg tcaaatggtt
    38521 gaatttaacg gtcttggaca ctcagcagct atccacacag ctgacgaaga attgactaaa
    38581 gaatttggta aagctgttaa agctattcgt gttatctgta actcaccttc tacttttggt
    38641 ggtatcgggg acgtttacaa tgccttcttg ccatcattga cacttggatg tggttcttac
    38701 ggacgcaact cagttgggga taacgttagt gccattaacc tcttgaatat caaaaaagtc
    38761 ggaagacgga gaaataacat gcaatggatg aaacttcctt caaaaacata ctttgaacgt
    38821 gattcaattc aataccttca aaaatgtcgt gacgttgaac gtgtcatgat cgttactgac
    38881 catgccatgg tagagcttgg tttccttgat cgtatcatcg aacaactgga ccttcgtcgc
    38941 aataaggttg tttaccaaat ctttgcggat gtagaaccgg atccagatat cacaactgta
    39001 aaccgtggta ctgagattat gcgtgccttc aaaccagata ccatcatcgc actcggtggt
    39061 gggtctccaa tggatgctgc caaagtaatg tggctcttct acgagcaacc agaagtggac
    39121 ttccgtgacc ttgtccaaaa attcatggat atccgtaaac gtgccttcaa gttcccattg
    39181 cttggtaaga agactaaatt catcgcgatt ccaactacat ctggtacagg atctgaagta
    39241 acaccatttg ccgttatctc tgataaagca aacaaccgta aatacccaat cgctgactac
    39301 tcattgacac caactgtggc aatcgtagat cctgctttgg tattgacagt tccaggattt
    39361 gttgctgctg atactggtat ggacgtattg actcacgcga cagaagcata cgtatcacaa
    39421 atggctagtg actacactga tggtttagca cttcaagcca ttaaattggt ctttgaaaat
    39481 ctcgaaagct cagttaagaa tgcagacttc cactcacgtg agaaaatgca taacgcttca
    39541 acaatcgctg gtatggcctt tgccaatgcc ttcctaggta tttctcactc aatggcccat
    39601 aagattggtg cgcaattcca cacaatccac ggtcgtacaa atgctatctt gcttccatac
    39661 gttatccgtt acaacggtac acgtccagct aagacagcaa catggcctaa gtacaactac
    39721 taccgtgcag atgaaaaata ccaagatatc gcacgcatgc ttggacttcc agcttctact
    39781 ccagaagaag gggttgaatc ttacgcaaaa gctgtctacg aactcggtga acgtattggg
    39841 atccaaatga attttagaga ccaaggaatt gacgaaaaag aatggaaaga acattctcgt
    39901 gaattagcct tcctggctta tgaagaccaa tgttcaccag ctaacccacg tcttccaatg
    39961 gtagaccata tgcaagaaat catcgaagat gcatactatg gctacaaaga aagaccagga
    40021 cgccgtaaat aattgtttat cagtctagaa gcaagacaaa aactcaattt gagggaaaga
    40081 tccagtaatt tttctatgat aaaaggcatc ctatcaaggt ttttgaacac ctgataggat
    40141 gcctttttat gatattgagg cctttttgcc ctttttgaaa aactagaata gaaacaaaat
    40201 atataataga ttgaaactag agtagtacac atctgcttct aaaacattgt tagaattcga
    40261 tttgactgtc
//
LOCUS       DAVKQL010000012        40161 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVKQL010000012 DAVKQL010000000
VERSION     DAVKQL010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40161
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.012"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            766..2919
                     /locus_tag="VW561_000550"
     CDS             766..2919
                     /locus_tag="VW561_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEU9942866.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPYKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTGINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNINLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLIVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2932..4281
                     /locus_tag="VW561_000551"
     CDS             2932..4281
                     /locus_tag="VW561_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEU9942867.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTVKSAIETGASLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4451..5158
                     /locus_tag="VW561_000552"
     CDS             4451..5158
                     /locus_tag="VW561_000552"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU9942868.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQELK"
     gene            5360..9085
                     /locus_tag="VW561_000553"
     CDS             5360..9085
                     /locus_tag="VW561_000553"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEU9942869.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLTEDLFAPAEKHIFSEQGTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDAFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTVFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            9178..10620
                     /locus_tag="VW561_000554"
     CDS             9178..10620
                     /locus_tag="VW561_000554"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU9942870.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     CDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDSLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10657..11679
                     /locus_tag="VW561_000555"
     CDS             10657..11679
                     /locus_tag="VW561_000555"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEU9942871.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKTLEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11676..12221
                     /gene="purN"
                     /locus_tag="VW561_000556"
     CDS             11676..12221
                     /gene="purN"
                     /locus_tag="VW561_000556"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEU9942872.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKADYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            12305..12814
                     /locus_tag="VW561_000557"
     CDS             12305..12814
                     /locus_tag="VW561_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU9942873.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLIFFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVRVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12839..14386
                     /gene="purH"
                     /locus_tag="VW561_000558"
     CDS             12839..14386
                     /gene="purH"
                     /locus_tag="VW561_000558"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU9942874.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            14508..15770
                     /gene="purD"
                     /locus_tag="VW561_000559"
     CDS             14508..15770
                     /gene="purD"
                     /locus_tag="VW561_000559"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEU9942875.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVKKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            16173..16661
                     /gene="purE"
                     /locus_tag="VW561_000560"
     CDS             16173..16661
                     /gene="purE"
                     /locus_tag="VW561_000560"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEU9942876.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTILPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16648..17739
                     /gene="purK"
                     /locus_tag="VW561_000561"
     CDS             16648..17739
                     /gene="purK"
                     /locus_tag="VW561_000561"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEU9942877.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLVDKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            17749..17976
                     /locus_tag="VW561_000562"
                     /pseudo
     CDS             17749..17976
                     /locus_tag="VW561_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
     gene            18039..19337
                     /gene="purB"
                     /locus_tag="VW561_000563"
     CDS             18039..19337
                     /gene="purB"
                     /locus_tag="VW561_000563"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU9942878.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(19392..23318)
                     /gene="strH"
                     /locus_tag="VW561_000564"
     CDS             complement(19392..23318)
                     /gene="strH"
                     /locus_tag="VW561_000564"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU9942879.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVTAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLDDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYILGQKPEDGGG
                     FLKKAIENTGKTPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEILSQPAPAEESK
                     ALPQDPAPVVIEKKLPETGTHDSAGLVVAGLMATLAAYGLTKRKED"
     gene            complement(23633..24349)
                     /locus_tag="VW561_000565"
     CDS             complement(23633..24349)
                     /locus_tag="VW561_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9942880.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            24697..26484
                     /locus_tag="VW561_000566"
     CDS             24697..26484
                     /locus_tag="VW561_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEU9942881.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFDGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVSRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            26481..26957
                     /locus_tag="VW561_000567"
     CDS             26481..26957
                     /locus_tag="VW561_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU9942882.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            26985..27890
                     /locus_tag="VW561_000568"
     CDS             26985..27890
                     /locus_tag="VW561_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9942883.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            27877..28692
                     /locus_tag="VW561_000569"
     CDS             27877..28692
                     /locus_tag="VW561_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9942884.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            28699..29103
                     /locus_tag="VW561_000570"
     CDS             28699..29103
                     /locus_tag="VW561_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9942885.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNMVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKIRAAESIVKVNDLLASFDDDEDE"
     gene            29397..30563
                     /locus_tag="VW561_000571"
     CDS             29397..30563
                     /locus_tag="VW561_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU9942886.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            30679..31716
                     /locus_tag="VW561_000572"
     CDS             30679..31716
                     /locus_tag="VW561_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEU9942887.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHIYNGTLLKDIFAEEDEQIQLASGLDHPFALPVGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            31924..32778
                     /locus_tag="VW561_000573"
     CDS             31924..32778
                     /locus_tag="VW561_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9942888.1"
                     /translation="MTYEYKSHIYLAETALNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTRIRLAR"
     gene            32928..33185
                     /locus_tag="VW561_000574"
     CDS             32928..33185
                     /locus_tag="VW561_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEU9942889.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADIVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(33227..33589)
                     /locus_tag="VW561_000575"
     CDS             complement(33227..33589)
                     /locus_tag="VW561_000575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942890.1"
                     /translation="MKIKVQTRKLAAGCSKHCFKVVDGTDEVSSKHCFEVVDRTDEVS
                     NHIYGKVKLTWFEEIFEEYKSLHNKTHITKVLMNCTPKVRQKKSNFLGSVHSTPDTMR
                     FFNFKDFLQLLLTHIKLF"
     gene            complement(33643..33939)
                     /locus_tag="VW561_000576"
     CDS             complement(33643..33939)
                     /locus_tag="VW561_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9942891.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPDAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQGLTVTNVEGGMNAWPGQVE"
     gene            34161..34925
                     /locus_tag="VW561_000577"
     CDS             34161..34925
                     /locus_tag="VW561_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEU9942892.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGADCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            35490..36869
                     /locus_tag="VW561_000578"
     CDS             35490..36869
                     /locus_tag="VW561_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU9942893.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            36883..37548
                     /locus_tag="VW561_000579"
     CDS             36883..37548
                     /locus_tag="VW561_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEU9942894.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            37662..37757
                     /locus_tag="VW561_000580"
                     /pseudo
     CDS             37662..37757
                     /locus_tag="VW561_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            37903..38442
                     /locus_tag="VW561_000581"
     CDS             37903..38442
                     /locus_tag="VW561_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9942895.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKGHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQEFESKGQEVDVFVTNFVYEKEGQSCKKSMSYDSVLPVRQIFGWDQVGNFSKGQ
                     YIMMHSLIYRTDLLRASQF"
     gene            38509..38919
                     /locus_tag="VW561_000582"
     CDS             38509..38919
                     /locus_tag="VW561_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEU9942896.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSGTAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            39035..>40161
                     /gene="pavB"
                     /locus_tag="VW561_000583"
     CDS             39035..>40161
                     /gene="pavB"
                     /locus_tag="VW561_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEU9942897.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVEKAVKDNVKDSIDVPAAYLEKAKGEGPFTAGVN
                     HVIPYELFAGDGMLTRLLLKASDKAPWSDNGDAKNPALSPLG"
BASE COUNT        11068 a         7797 c         9348 g        11948 t
ORIGIN      
        1 atgaacaaat tgataaatta tctgaactat ctaatctttt tacaattaaa atagaaacaa
       61 tattgacagt gatttctgtt gttttcacat ttatagcaac tcttattgaa tatggatttt
      121 ctctctttct gattacatta ttttttgata agaataagag gaactttgga gatattcttt
      181 tatctaaaaa tataacctta agttttagca ttttactttg ttatttattt aatccatcgg
      241 atataaagtt tgcctatttc agcgtattga cagtattact aattgggata acaaattata
      301 cgagatttaa aaatttaaga ttgacagtag tgttttctgt accattttta tttaatgcta
      361 taatgtctat tataaatagt ttttagaaat actaaaattt ttttctttac ttaataatga
      421 tatgaaatca tttcggtttt gttaacttgt aattcttata ttttctaggt agaacgtttg
      481 ttctgcctat ttttttatcc aaaaagtgca gttgggaggg agataggctc atttgggaag
      541 gaagtccagt ttttgtttag tgattggggt aagatagttg ttatcagatg agtttatact
      601 cttcgaaaat caaattcaaa tcacgtcaac gtcgccttgc cgtatatatg tgactgactt
      661 cgtcagtcct atctacaacc tcaaaacagt gttttgagca gcctgcggct agtttcctag
      721 tttgctcttt gattttcatt gagtattagg gaaaaggaga tgaatatgaa atttgggaaa
      781 cgtcactatc gtccgcaagt ggatcagatg gactgcggtg tagcttcatt agccatggtt
      841 tttggctact atggtagtta ttattttttg gctcacttgc gagaattggc taagacgacc
      901 atggatggga cgacggcttt gggcttggtc aaggtggcag aggagattgg ttttgagacg
      961 cgagccatta aggcggatat gacgcttttt gacttgccgg atttgacttt tccttttgtt
     1021 gcccatgtgc ttaaggaagg gaaattgctc cactactatg tggtgactgg gcaggataag
     1081 gatagcattc atattgccga tccagatccc ggggtgaagt tgactaaact gccacgtgag
     1141 cgttttgagg aagaatggac aggagtgact ctttttatgg cacctagtcc agactataag
     1201 ccttataagg aacaaaaaaa tggtctgctc tcttttatcc ctatattagt gaagcagcgt
     1261 ggcttgattg ccaatatcgt tttggcaaca ctcttggtaa ccgggattaa cattgtgggt
     1321 tcttattatc tgcagtctat cattgatacc tatgtgccag atcagatgcg ttcgacacta
     1381 gggattattt ctattgggct ggtcatcgtc tacatcctcc agcaaatctt gtcttacgct
     1441 caggagtatc tcttgcttgt tttggggcaa cgcttgtcga ttgacgtgat tttgtcctat
     1501 atcaagcatg tttttcacct ccctatgtcc ttctttgcga cacgcaggac aggggagatc
     1561 gtgtctcgtt ttacagatgc taacagtatc atcgatgcgc tggcttcgac catcctttcg
     1621 attttcctag atgtgtcaac ggttgtcatt atttcccttg ttttattttc acaaaatatc
     1681 aatctctttt tcatgacttt attggcgctt cctatctaca cagtgattat ctttgccttt
     1741 atgaagccgt ttgaaaagat gaatcgggac accatggaag ccaatgcggt tctgtcttct
     1801 tctatcattg aggacatcaa tggtattgag actatcaagt ccttgaccag tgaaagtcag
     1861 cgttaccaaa aaattgacaa ggaatttgtg gattatctga agaaatcctt tacctatagt
     1921 cgagcagaga gtcagcaaaa ggctctgaaa aaggttgccc atctcttgct taatgtcggc
     1981 attctctgga tgggggctgt tctggtcatg gatggcaaga tgagtttggg gcagttgatt
     2041 acctataata ccttgctggt ttactttacc aatcctttgg aaaatatcat caatctgcaa
     2101 accaagcttc agacagcgca ggttgccaat aaccgtctaa atgaagtgta tctagtagct
     2161 tctgagtttg aggagaagaa aacagttgag gatttgagct tgatgaaggg agatatgacc
     2221 ttcaagcagg ttcattacaa gtatggctat ggtcgagacg tcttgtcgga tatcaattta
     2281 atcgttcccc aagggtctaa ggtggctttt gtggggattt cagggtcagg taagacgact
     2341 ttggccaaga tgatggttaa tttttacgac ccaagtcaag gggagattag tctgggtggt
     2401 gtcaatctca atcagattga taaaaaagcc ctgcgccagt acatcaacta tctgcctcaa
     2461 cagccctatg tctttaacgg aacgattttg gagaatcttc ttttgggagc caaggagggg
     2521 acgacacagg aagatatctt acgggcggtc gaattggcag agattcgaga ggatatcgag
     2581 cgcatgccac tgaattacca gacagaattg acttcggatg gggcagggat ttcaggtggt
     2641 caacgtcaga gaatcgcttt ggcgcgtgct ctcttgacag atgcgccggt cttgattttg
     2701 gatgaggcga ctagcagttt ggatattttg acagagaagc ggattgtcga taatctcatg
     2761 gctttggaca agaccttgat tttcattgct caccgcttga ctattgctga gcggacagag
     2821 aaggtagttg tcttggatca gggcaagatt gtcgaagaag gaaagcatgc tgatttgctt
     2881 gcacagggtg gcttttacgc ccatttggtc aatagctaga aagaggagag gatgaaacca
     2941 gaatttttag aaagtgcgga gttttataat cgtcgttacc ataatttttc cagtagtgtg
     3001 attgtaccca tggcccttct gctcgtgttt ttacttggct ttgcaactgt tgcagaaaag
     3061 gagatgagtt tgtccactag agctactgtc gaacctagtc gtatccttgc aaatatccag
     3121 tcaactagca acaatcgtat tcttgtcaat catttggaag aaaataagct ggttaagaag
     3181 ggggatcttt tggttcaata ccaagaaggg gcagagggtg tccaagcgga gtcctatgcc
     3241 agtcagttgg acatgctaaa ggatcaaaaa aagcaattgg agtatctgca aaagagcctg
     3301 caagaagggg agaaccactt tccagaggag gataagtttg gctaccaagc cacctttcgc
     3361 gactacatca gtcaagcagg cagtcttagg gctagtacat cgcaacaaaa tgagaccatc
     3421 gcgtcccaga atgcagcagc tagccaaacc caagccgaaa tcggcaacct catcagtcaa
     3481 acagaggcta aaattcgcga ttaccagaca gttaagtcag ctattgaaac aggtgcttcc
     3541 ttggccggtc agaatctagc ctactctctt taccagtcct ataagtctca gggcgaggaa
     3601 aatccccaaa ctaaggttca ggcagttgca caggttgaag cacagatttc tcagttagaa
     3661 tctagtcttg ctacttaccg tgtccagtat gcaggttcag gtacccagca agcctatgcg
     3721 tcagggttaa gcagtcaatt ggaatccctt aaatcccaac acttggcaaa ggttggtcag
     3781 gaattgaccc ttctagccca gaaaatcttg gaggcagagt caggtaagaa ggtacaggga
     3841 aatcttttag acaaggggaa aattacggca agtgaggatg gggtgcttca tcttaatcct
     3901 gagaccagtg attctagcat ggttgcagaa ggtgccctac tagcccaact ttatccatct
     3961 ttggaaagag aagggaaagc caaactcaca gcctatctaa gttcaaaaga tgtagcaaga
     4021 atcaaggtcg gtgattctgt tcgctatact acgactcatg atgccgggaa tcaacttttt
     4081 ctagattcta ctattacaag tattgatgcg acagctacta agactgagaa agggaatttc
     4141 tttaaaatcg aggcggagac taatctaact tcggagcagg ctgaaaaact taggtacggg
     4201 gtggaaggcc gtctacagat gattacgggc aagaaaagtt atctacgtta ttatttggat
     4261 caatttttga acaaagagta atgttcgtgt ttttagagtt aaataatttt taaactgtga
     4321 gaaagattct tcttgcagtt ttttctttac aatttttgaa aaacatctgc tatttattcg
     4381 gttaaattct tgtgtttttt ggttttttgt ggtaaaatgt gctcaagtaa tacgaaaggc
     4441 gaactttaaa atgtcaaaac aattgatcta ttcgggaaaa gctaaagata tctatacaac
     4501 tgaggatgaa aatcttatta tttcaactta caaggaccag gcgactgctt tcaacggtgt
     4561 caagaaggag cagattgcag gtaagggagt cttgaataat cagatctcat cttttatttt
     4621 tgagaaatta aatgcggctg gtgtggcgac tcactttgtg gagaaacttt cagacacgga
     4681 acaactcaat aaaaaggtta agattattcc tttggaagtc gtgctccgca actatactgc
     4741 tggttccttt tcaaaacgtt ttggtgtgga tgagggaatc gccttggaga ctccgattgt
     4801 cgaattttac tacaaaaatg atgatttgga tgatccattt atcaatgatg agcatgtgaa
     4861 attcctacag attgcgggtg accagcagat tgcctacttg aaggaagaaa cgcgtcgtat
     4921 caatgaacta ttgaaagtct ggtttgctga gattgggctt aaattgattg actttaagct
     4981 agagttcggt tttgacaagg atggcaagat tatcttggca gacgaatttt caccagataa
     5041 ctgccgcttg tgggacgctg atggcaacca catggataag gatgttttcc gtagaggatt
     5101 gggagaacta accgacgttt atgagattgt ttgggaaaag ttgcaggaat tgaaataatc
     5161 tgtttgcaac ggaaaacctt cgtctctcaa ctaaaaggac tcaggctgaa aaggtccccc
     5221 agaccttttc actctgtaga gaactaggtg aactaacaga tgtttacgaa attgtctggg
     5281 aaaagttgca gggtttaaaa taacaacctc aaggctgttt gggaatattg caagagctga
     5341 aataaaggaa taagaattga tggataaacg tatttttgtt gaaaaaaagg ctgattttca
     5401 ggtcaagtca gagagtttgg ttagagagct ccagcacaac ttgggactgt caagcttgaa
     5461 aagtattcgt attgtgcaag tatatgatgt atttgacttg actgaggact tgtttgcacc
     5521 tgcagagaag cacattttct ctgagcaggg aaccgaccat gttttagatg aagtatctgt
     5581 gcaggcggat cttgctaact atgctttctt tgccattgaa agtctgccag ggcagtttga
     5641 ccagcgtgca gcttcgtcac aggaagcctt gcttttgttg ggaagttcga gtgacgtgac
     5701 agtcaacaca gcccaacttt acttggtgaa taaagatatt gatgcgactg agttggaagc
     5761 tgtcaaaaac tacctgctca atccagttga ttctcgtttc aaggatatca cgacagggat
     5821 tgccaagcag gagttttcag agtcagacaa gaccattcca aaattgactt tctttgaaag
     5881 ctatacagca gaagactttg ctcgctacaa ggccgagcaa gggatggcca tggaagtgga
     5941 tgatttgctc tttatccaag actactttaa gtcaatcggg cgcgtgccaa ctgagactga
     6001 actcaaggtt ttggacactt actggtctga ccactgccgt catacgactt ttgagacaga
     6061 gttgaaacac atcgactttt cagcttctaa atttcaaaag caattgcagt caacctatga
     6121 caagtatatt gccatgcgcg aggagttagg tcggtctgaa aaaccacaaa ccttgatgga
     6181 tatggcgact attttcggtc gttatgagcg tgctaacgga cgattggatg atatggaagt
     6241 ctctgacgaa atcaatgcct gctcagttga aattgaagtg gatgttgatg gtgtcaagga
     6301 accttggctc ctcatgttta aaaatgaaac ccacaaccat ccaacagaaa ttgagccatt
     6361 tggtggagcg gctacctgta tcggtggagc tattcgtgat ccgttgtcag gccgttccta
     6421 tgtttaccaa gccatgcgta tttcaggtgc tggtgatatt acagcaccga tttcggaaac
     6481 tcgtgctggg aaattgccac aacaagtcat ttctaaaaca gcagctcatg gttattcttc
     6541 atatggtaac cagattgggc ttgccacaac ctacgttcgt gaatacttcc acccaggctt
     6601 tgtagctaaa cgtatggaac ttggtgccgt tgttggagcg gctcccaagg gcaatgttgt
     6661 ccgtgaaaaa cctgaagcag gtgatgtgat catccttctc ggaggcaaaa caggtcgtga
     6721 tggtgtcggt ggtgcgacgg gctcttctaa ggttcaaaca gttgagtctg tagagactgc
     6781 tggtgctgag gttcaaaaag gaaatgccat cgaagaacgc aagattcagc gcctcttccg
     6841 taatggcaat gtcactcgtc tgatcaagaa gtccaatgac tttggggcag gcggcgtctg
     6901 tgtggctatc ggtgaattgg cagacggtct tgaaatcgac ctcaacaagg tgcctcttaa
     6961 ataccagggc ttgaatggta cagaaattgc catctctgaa tcacaagaac ggatggcggt
     7021 cgtggttcgt cctgaagatg tggatgcctt cgttgccgaa tgtaacaaag aaaatattga
     7081 tgctgttgtg gtggcgacag taactgaaaa accaaatctt gtcatgcact ggaatggtga
     7141 gacaatcgtt gacttggagc gtcgtttcct tgacaccaat ggtgtgcgcg tggttgtcga
     7201 tgccaaagtt gtggacaagg atgtcaaact cccagaagag cgtcaaacat ctgctgaaac
     7261 actggaatca gataccctta cggttctatc tgacctcaac catgcaagtc aaaaaggatt
     7321 acagactgtc tttgactgct ctgttggacg ctcaacggtt aatcacccac ttggtggtcg
     7381 ttaccaactc acaccaactg aggcatctgt gcagaaattg ccagttcaac acggtgtgac
     7441 tcatactgcg tcggtcattg ctcaaggttt caacccatat gtagctgaat ggtctccata
     7501 ccacggtgct gcttacgcgg ttatcgaagc aactgctcgt ttggtggctg ctggtgccaa
     7561 ctggtctaag gctcgtttct cttaccaaga atatttcgag cggatggaca agcaagcaga
     7621 gcgttttggt cagccagtag ccgcccttct aggctctatt gaagctcaga ttcaacttgg
     7681 cttgccatct atcggtggta aggactccat gtctggtacc tttgaagaat tgaccgttcc
     7741 gccaaccttg gttgcctttg gggtgacgac ggcagatagc cgtaaggtgc tctctccaga
     7801 atttaaagct gttggggaaa atatctacta catcccaggt caagccctct ctgcagagat
     7861 tgattttgac ttgattaaga aaaattttgc tcagtttgaa gcaagccaag ctgaccataa
     7921 agtgacatct gcatcagctg tcaaatacgg tggtgtagtt gaaagtttgg ctcttgctac
     7981 ctttggaaac tatattggtg cagaggtgac cttgcctgaa cttaaaacag ctttgacagc
     8041 tcaattaggc ggctttgtct tcacatctcc tgaagaaatt gctggagtag agaagattgg
     8101 acaaacgaaa gcagacttta cactgactgt caacggtgtg aagctagatg gacacaagct
     8161 tgacagtgca tttcaaggga cattggaaga agtttaccca acagaattta cccaagcgaa
     8221 agaactagaa gaagtaccag ctgtggcatc agatgttgtg attaaagcca aagaaaaggt
     8281 tgaaaaacct gtggtttaca tcccagtctt tccaggaacc aactcagaat atgattcagc
     8341 taaggccttc gaaaaagaag gtgcagaggt caatttggtg ccattcgtga ccttgaatga
     8401 agaagctatt gtcaagtcag ttgaaactat ggttgacaat atcgacaaga ctaatattct
     8461 cttctttgcc ggtggattct cggctgcgga tgaaccagat ggttcagcta agtttatcgt
     8521 caatatcctg cttaatgaaa aagtgcgtgt ggctattgat agctttatcg cccgtggtgg
     8581 cttgattatc ggtatttgta atggattcca agccttagtc aaatcgggtc tcctaccata
     8641 cggaaacttt gaagatgcta acagtactag tccaaccctc ttctacaatg atgccaacca
     8701 acacgtggcc aagatggtgg aaactcgcat tgccaatacc aactcaccat ggttggctgg
     8761 tgtgcaagtg ggcgatatcc acgctattcc tgtttcgcac ggtgaaggga agtttgtcgt
     8821 gacggctgag gaatttgcag agctccgtga caatggacaa attttcagcc aatacgttga
     8881 ctttaacggt aaaccaagta tggattctaa gtacaatccg aatggttctg tccatgccat
     8941 cgaaggaatt accagcaaga atggtcaaat catcggtaag atgggccact cagaacgtta
     9001 tgaggatggt cttttccaaa atatcccagg caataaagat caacacctgt tcgcatcagc
     9061 ggttaaacat ttcactggaa aataagactt acagattttc taatagatag tatcagtaat
     9121 gtaaaagtca tgtaaatcta gctcttgatg attacaaatg aaaattaggt ataaaaaatg
     9181 acatacgaag taaaatctct taatgaagaa tgtggtgttt tcggtatttg gggacatcca
     9241 gatgctgcta agttgaccta ttttggactc cacagtcttc aacaccgtgg tcaggagggg
     9301 gcaggaatcc tctccaacga tcaaggacaa ctgaagcgcc atcgtgacat ggggctttta
     9361 tcagaagttt tcagaaatcc agctaatttg gataaattga caggagctgg tgcgattggg
     9421 catgtgcgtt atgcgactgc tggcgaagct tctgtagata acatccagcc cttcctcttc
     9481 cgttttcacg atatgcagtt tggcttggct cataatggaa atctgaccaa tgcagcctct
     9541 ctcaagaaag aattggaaca aagaggagca attttcagcg cgacttcgga ctctgaaatc
     9601 ttggctcacc tcattcgtcg cagtcataat cctagcctga tgggcaaaat caaggaagcg
     9661 ctcagccttg tcaaaggtgg ttttgcctat atcttgctgt ttgaggacaa gttgattgcg
     9721 gctcttgacc ctaatggctt tcgtccgctt tctatcggga aaatggccaa cggagcggtg
     9781 gttgtttcct ctgaaacctg tgcttttgag gtcattggtg ccgagtggat ttgtgatttg
     9841 aagccaggtg agattgtgat cattgatgac gagggcattc agtatgacag ctatacagat
     9901 gatacccagt tggcgatttg ttctatggag tatatctact ttgcccgccc tgattctaat
     9961 atccacggtg tcaatgtcca tacggcacgt aagagaatgg gagcgcaatt ggcgcgagaa
    10021 tttaagcatg aggcagatat tgtagttggt gtgcccaatt cttccctaag cgcggctatg
    10081 ggatttgcgg aagaatcagg cttaccaaat gaaatgggtc tgatcaaaaa ccaatacacc
    10141 cagcgaactt ttatccaacc gactcaagaa ttgcgggagc aaggagtgcg gatgaaactg
    10201 tctgctgttt cgggtgttgt caaaggcaaa cgtgtggtca tggtggatga ttccattgta
    10261 cgtggaacaa cttctcgtcg tatcgttcag ctcttgaaag aagcgggtgc gactgaggtt
    10321 cacgttgcca ttggaagtcc tgcactagcg tatccatgtt tctacgggat tgatatccag
    10381 acccgtcagg agctgattgc agccaatcat acggtcgaag aaactcgcca aatcattggt
    10441 gcggacagtc tgacttatct ttcaattgat agcttgattg agtcgattgg tatcgaaaca
    10501 gatgcgccga acggtggtct ctgtgtcgct tactttgacg gtgactaccc aacgcctctc
    10561 tatgactacg aagaagacta tcgtagaagt ttggaagaaa agaccagttt ttacaagtag
    10621 gcgacagatt ctccattaaa gaaaaggaaa aaacaaatga caaataaaaa tgcatatgcc
    10681 cagtcgggtg tggatgttga agcgggttat gaagttgttg aacggattaa aaagcacgtg
    10741 gcccgtacgg agcgtgcagg tgtcatggga gctcttggtg gctttggtgg tatgtttgac
    10801 ctttccaaga ctggggttaa agaacccgtc ttgatttcag ggactgacgg tgtcggaacc
    10861 aagctcatgt tggctatcaa gtacgacaag cacgatacca tcgggcagga ctgtgtggcc
    10921 atgtgtgtca acgacatcat tgctgcaggt gcggaacctc tctattttct cgactacgta
    10981 gcgacaggga agaatgaacc agctaagcta gaacaagtgg ttgctggtgt ggcagaaggt
    11041 tgtgtgcagg ctggtgctgc cctcatcggt ggggaaacgg ctgaaatgcc gggcatgtac
    11101 ggcgaagacg actatgactt ggctggtttt gcggtcggtg tggctgaaaa atctcaaatc
    11161 attgacggtt caaaggtggt agagggagat gttcttctcg gacttgcttc aagtgggatt
    11221 cactcaaatg gttactcttt ggttcgtcgt gtctttgcgg attacacagg tgaggaagtc
    11281 ctaccagaat tggaaggcaa gaaacttaag gaagttctac ttgagccgac tcgtatctat
    11341 gtcaaggctg tcttgccact catcaaagaa gagttggtca acggcattgc ccacatcaca
    11401 ggtggtggct ttatcgaaaa tgtccctcgt atgtttgcag atgacctagc tgctgaaatt
    11461 gatgaaagca aagttccagt gcttccaatt ttcaaaaccc ttgaaaaata cggtcagatt
    11521 aaacacgaag aaatgtttga aatcttcaat atgggtgtgg gacttatgtt ggcggtcagc
    11581 cctgaaaatg tagagcgtgt aaaagaattg ttggatgaag cagtctatga aattggtcgc
    11641 atcgtcaaga aagaaaacga aagtgtcatt atcaaatgaa aaaaatagcg gtttttgcct
    11701 ctggtaatgg ctcaaatttt caggtgattg ccgaagaatt tccagtggag tttgtctttt
    11761 cagaccatcg tgatgcctat gtgcttgagc gtgcaaagca gctcggcgtt ctgtcctatg
    11821 cttttgaact caaggagttt gagagcaagg cagactacga agcagccctt gtcgaactct
    11881 tggaagaaca ccagattgac ttggtttgcc tagcaggcta catgaaaatc gttggaccaa
    11941 ccttattgtc ggcttatgaa ggtcggattg tcaacattca tccagcctac ttgccagaat
    12001 ttccaggagc tcatgggatt gaggatgctt ggaatgctgg cgtgggtcag tctggtgtga
    12061 ccattcactg ggtggattcg ggtgtggata caggccaggt catcaaacag gttcgtgtgc
    12121 cacgactagc tgatgatacc attgacagat ttgaagctcg catccatgaa gcagagtaca
    12181 ggttgtatcc ggaagtagtg aaggctctat ttacagattg actttttgat gattcatatg
    12241 atatctttga ttttaaattg gagtcagtgt ttgttgaaga cggcttcaaa cggaggtatt
    12301 tgtaatgtta gaatctaaaa aaacaactcg atatgtattt tatgtctatc tgatgttatt
    12361 aacttgggga atcttattta agtttgaaac aaatcctgaa tttatagcat ttttcttagc
    12421 tccaaggtat atcaattgga ttccattttc agaaccacta atagtcgatg gaaaaattgt
    12481 ttttgctgaa atgttattta atctgatttt ctttattcca ttaggtgttt gtttcccttt
    12541 gataaaaact aatttatcta gtttaagaat agtcgggaca ggtttcttga ttagtttatt
    12601 gtttgagtgc ttacagtata ttttagcaat aggtataaca gatataacgg atttgacttt
    12661 aaatacgcta ggtgtccgtg taggcttact gatttatcaa atttttataa gagtgttcaa
    12721 atcacagact agaaaatgga tcaatatctt aggtatgctt agccttggtt ttgcttatct
    12781 tgttttactg ttactgcatt taattggtgt ttaactaatg attaaaaagg agaatataat
    12841 gactaaacgc gtcttaatca gcgtctcaga caaagcgggc attgttgaat ttgcccaaga
    12901 actcaaaaaa cttggttggg agattatctc aacaggtgga actaaggttg cccttgataa
    12961 tgctggggtg gataccattg ctatcgatga tgtgactggt ttcccagaaa tgatggacgg
    13021 tcgtgtgaag accctccacc caaatatcca cggagggctt ctcgctcgtc gtgacttgga
    13081 tagccacttg gaagcggcta aggacaacaa gattgagctc attgaccttg tggtggtcaa
    13141 cctttaccca tttaaggaaa ctatccttaa accagatgtg acttatgctg atgcagttga
    13201 aaatatcgat attggtgggc catctatgct tcgttcagca gcgaaaaatc atgccagtgt
    13261 tacagttgtg gtagatcctg ctgactacgc tgtggttttg gacgaattgg cagcaaacgg
    13321 cgaaacctct tatgaaactc gccaacgttt agcagccaaa gtatttcgtc acacagcggc
    13381 ttatgacgcc ttgattgcag aatacttcac agctcaagtg ggtgaaagca agcctgaaaa
    13441 actcactttg acttatgacc tcaagcaacc aatgcgttac ggtgagaatc ctcaacaaga
    13501 cgcggacttt taccagaaag ctttgcctac agactactcc attgcttcag ccaaacagct
    13561 caacgggaaa gaattgtcat ttaataatat ccgtgatgca gatgctgcta tccgtatcat
    13621 ccgtgacttc aaagatagtc caaccgttgt ggctctcaaa cacatgaatc catgtggaat
    13681 tggtcaagct gatgacatcg agactgcttg ggactacgct tatgagtctg acccagtgtc
    13741 tatctttggt gggattgtcg tcctcaaccg tgaggtggat gctgcgacag ctgagaagat
    13801 gcacggcgtt ttcctcgaaa tcatcattgc accaagctat acggatgaag cgctagccat
    13861 tttgatcaat aaaaagaaaa acttgcgtat ccttgccttg ccatttaatg ctcaagaggc
    13921 tagcgaagtg gaagcagaat acacaggtgt agtcggtgga cttctcgtgc aaaatcaaga
    13981 cgtggtcaag gaaagcccag ctgactggca agtggtgact aaacgtcagc caactgagac
    14041 agaagcgact gctcttgagt tcgcttggaa ggctatcaag tacgtcaaat caaatggtat
    14101 tatcgtgacc aacgaccaca tgacacttgg tgttggtcca ggtcaaacca accgtgtggc
    14161 ttctgttcgc cttgccattg accaagccaa agatcgtctg gacggggcgg tccttgcttc
    14221 agatgccttc ttcccatttg cggataacgt ggaagaaatc gccaaagcag gaattaaggc
    14281 catcatccag cccggtggct ctgtccgtga ccaagaatct atcgaagcgg cagacaaata
    14341 tggcttgacc atggtcttta caggtgtgag acattttaga cattaagaag ataaaaggga
    14401 agaaaacagt ttctttcctt ttttggctta aaatgctaac tgaaacaaga ttaaaacgaa
    14461 cttttttgat ataatgttgg taaataattc gcaaaatagg ttgaggaatg aaactgcttg
    14521 ttgtcggttc tggtggtcgt gagcatgcga ttgctaaaaa gttacttgaa tcaaaagacg
    14581 tgaaaaaagt ctttgtagct cctgggaatg atgggatgac tctggatggt ttggaattgg
    14641 taaatatctc tatttccgaa cattctaaat tgattgactt cgcaaagacc aatgatgttg
    14701 cttggacctt tatcggtcca gatgatgccc ttgctgctgg tatcgtggat gattttaacc
    14761 aagctgggct taaggccttt ggtccgacta gggctgcagc ggagctggag tggtccaagg
    14821 atttcgccaa ggaaatcatg gtcaaatatg gtgtttcgac agcagcctat ggcacatttt
    14881 cagatttcga ggaagcaaag gcctatatcg aaaagcatgg tgcgcctatc gtagtcaagg
    14941 cggatggctt ggcgcttggg aagggtgtcg tcgttgctga gacggttgag caagcggtcg
    15001 aagccgctca tgagatgctt ttggacaata aatttggtga ctcaggtgcg cgcgtggtta
    15061 ttgaggaatt ccttgaagga gaggaatttt cactctttgc ctttgtcaat ggtgataagt
    15121 tctacatcat gccaacggct caggaccaca aacgtgccta cgatggcgac aaagggccta
    15181 acacgggtgg tatgggtgcc tatgcgccag ttccacactt accacagagt gtagttgata
    15241 cagcggttga caccattgtc aagccagttc tagaagggat gattaaagaa ggtcgccctt
    15301 atctgggagt tctttacgca gggcttatcc tgacagctga tggaccgaaa gtcattgagt
    15361 tcaacgctcg gttcggagat ccagaaactc agattatctt gcctcgcttg acctctgact
    15421 ttgctcaaaa tatcacagat atcctggata gcaaggagcc aaatatcatg tggacggaca
    15481 agggtgtgac tctgggtgtg gttgtcgcat ccaagggcta cccgctagac tatgaaaggg
    15541 gcgttgagtt gccagccaag acagaaggcg atgtcatcac ctactatgca ggggctaagt
    15601 ttgcggaaaa tagcagagca ctgctctcaa acggcggacg agtttatatg ctggttacca
    15661 cagcagatac cgtcaaagaa gcccaagcca gcatctacca agaactatcc caacaaaaaa
    15721 tagaaggact cttctaccga acagatatcg gaagcaaggc aattaagtaa agatataaga
    15781 ataacgcgcc gtagtcgcca aacacgataa tggtcgtcgt ggtgaaaaga ccagaacagt
    15841 gaatgttctg gtcaggggga aacttggaga ccgtaggctc aaagtttagg aatgaaaccg
    15901 aaggtttgct tccgcctcca tcacctaaga ccattatcaa aaagaaaaat aaaaattcac
    15961 aaaatacgtt aatgatcgta tggtttgcga gcgttaacga gctaatatag aacaatcacc
    16021 gccgttgtga aagaacgatt ggatgataat ccaatcgttc agggaaattg gaagaccttg
    16081 ggtttccaat ttaggcatga gacacctttg gtggctgctg ccgtccctca caagctaagg
    16141 tgattgttga aaaagaggaa aaaggagaag aaatgaaacc agtaatttcc atcatcatgg
    16201 gctcaaaatc cgactgggca accatgcaaa aaacagcaga agtcctagac cgcttcggtg
    16261 tagcctacga aaagaaagtt gtttccgccc accgtacacc agacctcatg ttcaaacatg
    16321 cagaagaagc ccgtagccgt ggcatcaaga tcatcatcgc aggtgctggt ggcgcagccc
    16381 atttgccagg tatggtcgct gctaaaacaa tccttccagt cattggtgta cctgtcaagt
    16441 ctcgtgctct tagtggagtg gattcactct attctatcgt tcagatgccg ggtggggtgc
    16501 ctgttgcgac catggctata ggtgaagctg gagcgactaa cgcagctctc tttgccctcc
    16561 gtctcctctc tgtagaagat aagtccattg cggatgcact tgccaacttt gctgaagaac
    16621 aaggaaaaat cgcagaggag tcgtcaaatg agctcatcta aaacaatcgg aattatcggt
    16681 ggcggtcaac tgggtcagat gatggccatt tctgctatct acatgggcca caaggttatc
    16741 gcgctggatc ctgcggcgga ttgcccggcc tctcgcgtgg cggaaatcat tgtggcacct
    16801 tataacgatg tggacgccct ccgtcagttg gcagaccgtt gcgatgtcct cacttacgag
    16861 tttgaaaatg tcgacgctga cggtttggat gccgttatca aggatggaca actccctcaa
    16921 ggaacagatc tgctccgcat ttcgcaaaat cgtatttttg aaaaggactt tttgtcaaac
    16981 aaggctcaag tcactgtggc accctacaag gtcgtgactt ctagcctaga cttggcagat
    17041 atcgacttgt cgaaaaacta tgtcctcaag actgcgactg gtggctacga tggtcatgga
    17101 caaaaggtta ttcgttcaga agcagacttg gaagcagcct atgcgctagc agactcagca
    17161 gactgcgtct tggaagaatt tgtcaacttt gaccttgaga tttctgtcat cgtgtcagga
    17221 aatggcaagg aggtgacgtt tttcccagtt caggaaaata tccaccgcaa caatatcctg
    17281 tctaagacca tcgtaccagc ccgcatttct gaaagtctag tagacaaggc taaagctatg
    17341 gcagtgcgaa tcgcagaaca actcaacttg tctggaactc tctgtgtgga aatgtttgcg
    17401 actgatgatg acgttattgt caatgaaatc gccccacgac cacataactc tgggcactat
    17461 tctattgaag cctgtgattt ctctcagttt gacacccata ttctcggtgt tctcggagca
    17521 ccattaccag tcatcaaact ccatgcgcca gccgttatgc ttaatgtcct cggtcagcat
    17581 gtcgaggctg ctgagcaata tgtgacagaa aattcaagcg cccacctcca catgtatggt
    17641 aaaatagaag cgaagcacaa ccgcaagatg ggacatgtga ccttgtttag tgatgtaccg
    17701 gatagtgtgg atgagcttgg ggaagggatt gatttttagg acaagtctat gatacaaatt
    17761 atcgttaata catttattga aaaggataag actggagcag ttgttgaagt gttgtatgcc
    17821 agtgctgacc aagataaggt acaagctaaa tatgaagaac tagctgcata ataccccgaa
    17881 aattatttag ctatctatga tgtaccgctg gatacggatt tgaatacact agatcattac
    17941 ccgtctgtgt ttattgaaaa agaggagttt gagtagaaat cttggtttac ctagatagct
    18001 tattcccaac agcttaagaa gaaaggaaaa attaacacat gatcaaccgt tactctcgcc
    18061 ctgagatggc gaatatttgg agtgaagaaa ataaataccg tgcttggctt gaggtggaaa
    18121 tcctctctga cgaggcatgg gctgagttgg gggaaatccc taaggaagat gtggctttga
    18181 ttcgcaagaa ggcggacttt gacatcgacc gtattttgga aattgagcag gagacgcgcc
    18241 acgatgtggt ggctttcacg cgtgcggttt ctgagactct tggtgaagag cgcaagtggg
    18301 ttcactatgg gttaacttct accgacgtgg tggatactgc ttatggttac ctctacaagc
    18361 aggccaacga catcatccgt cgtgaccttg aaaacttcac taatatcatc gctgacaagg
    18421 ccaaggagca caagttcacc atcatgatgg ggcgtactca tggtgtgcac gctgagccga
    18481 caacctttgg tcttaaattg gcaacttggt acagcgaaat gaaacgcaat atcgagcgtt
    18541 tcgagcatgc agctgctggt gtagaagctg gtaagatttc tggtgcggtt gggaactttg
    18601 ccaatatccc accatttgta gaggagtatg tctgcgataa acttggcatc cgtgcccaag
    18661 aaatctctac acaagtcctt cctcgtgacc ttcacgctga gtactttgcg gttcttgcca
    18721 gcattgcgac ttcaatcgaa cgtatggcga ctgagattcg tggtctacaa aaatctgagc
    18781 aacgcgaagt agaagagttc tttgctaaag ggcaaaaagg gtcttcagca atgcctcaca
    18841 aacgcaaccc aatcggttct gaaaatatga ctggtctggc gcgtgtcatt cgtggtcaca
    18901 tgattacggc ttatgaaaac gtcgctctct ggcacgaacg cgatatttcc cactcatcag
    18961 ctgagcgtat catcacacca gatacgacca ttttgattga ctacatgctc aaccgttttg
    19021 gaaatatcgt taagaacttg acagtcttcc cagaaaatat gatccgaaac atgaactcga
    19081 cttttggtct tatctttagc caacgggcta tgttgacatt gattgaaaaa ggcatgaccc
    19141 gtgagcaagc ctatgacttg gtgcaaccaa aaacagccta ctcttgggac aaccaagtag
    19201 actttaaacc acttcttgag gcagattcag aagtaacatc acgtctcaca caagaagaaa
    19261 tcgatgaaat cttcaaccca gtttattaca ccaaacgagt ggatgatatc tttgaacgtc
    19321 ttggactagg tgattaatta aaaaataaac agcgagcttc aatctcgctg tttatttttt
    19381 atcgaaaaga cttagtcttc ttttctttta gtgagtccat aggctgctag tgtggccatg
    19441 agtcctgcga ctactagtcc tgcagaatcg tgagttcctg tttcaggaag ttttttctct
    19501 attaccacag gagctggatc ttgaggaaga gctttgcttt cctcagcagg agctggttgg
    19561 cttaggattt ctagttttgg tttttcttca gcaatagcgg cttgtccgtt ttcatcgcct
    19621 acatgtgtta ccatagttcc gacttcgact atttgagtaa cggcttcctg tgctacgaca
    19681 ctatttacaa gtgttttcac ttccttacca tcggcagaag tgctcacaga gtagaagttg
    19741 ctacgatgtc cattgacacc cttagtaatg acttgtgttt ttcctttgag taagagtgga
    19801 ttttcacgag tcactgtggt aaatggaatt tcttcttttt ggatatccag tctaggtttt
    19861 acctcagtag ttggtgcaag accactttca tcacccttgt gagttacagg agcgccaact
    19921 tcaaccactt ggtttataac ttctttggtt acctggctat caaggactgt ttctgttgtt
    19981 tttccatttt cagtgagtac agagatgtaa tgagttcgtt cacctttgac tcctgctgtg
    20041 ataatatttt cctgaccagc tgggaggtta ggattttctt tcttgataac ttcaaatgga
    20101 atttcttcag ttcttgtgat gagttctggt ctggtttcaa cattggcagc cacttcattt
    20161 tcatctaggc ttcctgaatg agttgcagct ggtttgaggc cttgaagagc ggcttttagg
    20221 ttggctacaa gtgtgtcaag ctcagcttgt ttattacggt tgaggttgta atttagagct
    20281 gttttagctg cgtcaagggc ctcaagactt tctttactat atccttctaa gtttgtagga
    20341 attttagcta attcttcgcg gagagcatta taattagcac ggaagtagtc tttgttgtgg
    20401 tctgcaaagg cagtcatgag ttcaaagatt tcctcttcct tgtattcagc gcttggtcta
    20461 tctgcccaga ttgaaagcat acttccgact gttggaagat ctacttcagg atatttggta
    20521 gaagctagtt gattgaatgg tgtttttcca gtattctcaa tagctttctt gaggaaacca
    20581 ccaccatctt ctggtttttg accaagaatg tagtaccagt caccgttggt attcaagaat
    20641 ttatagcctt tgcttgctag gtattgaggt gatgcgaggt tatatcccca ccagccttta
    20701 gaccagtaag aaatcaagac atctttgtca aactgaacat cgtccttgtc ttcatagtag
    20761 aagccatcgt tgaaggccat tggttgaagc cctctttctt tggccatagc tgcgagggtg
    20821 ttggcatatt cggcaaattt gccatagagt tgataccact tgaggtagta ccagccttgg
    20881 gcactagtcg catcgttggc gtattcgtca gtaccaaagt tgaaaatctt tgttttacct
    20941 gcaaagaagt ccatgtattt accgatgagg gcttttacaa agttcatcgc ttcttcgttt
    21001 ttcaagtcca tagttgtttt tgaaacttta tcaaagtggg cttgaggatt tttaatacct
    21061 aatttttcca tggcaaccag catagcatcc atgtgacctg gactgttaat agctgggatg
    21121 agaccgatgt ccttagattt agcgtattca attagctctg ttacttctgc ctgtgttagt
    21181 gcagtaccgt ttggatcgtc gtagtaagct ttagttcctt cgataatagc ttttttaacg
    21241 tcatcactag cataggtttt tccgttggca gtaatggtca tatcatcgag tagaaagcga
    21301 agtccgtcat ttcctagaag gagatggaca tcagaatatc cgagctcact ggccttgtct
    21361 acgatgcgtt tgagctggtt cagagtaaag tatttgcgtc cagcatcgat tgagattacc
    21421 ttgtttttgg caagtttttc aacctcacgt ttagcttctt cttctttttg agcttcaggc
    21481 gtgagggtca agttgttgac agtttcttga agtttagcaa tggcttgatc aatcgtatct
    21541 tgttgggcac ggctaaggtt gctatcgaga gagcgaatag ctttttcagc ttcttttacg
    21601 gccgtgacgc tttctgcagt ataacggttc aggtcttttg gtacctcgtt aagtgcttgc
    21661 tctgcagatt cataatcagc tgcgaagtat tcagcgttgg catttgcaaa atgacgcatg
    21721 agtttgaaga ggcgtgatgg tgaataacgt gcagatggag tgtcagccca agcagctacc
    21781 ataccaccga tgattgggat atcagctcct tctgtttttg gtacagaagt gattggtgtg
    21841 tttttaatac cattgagccc ctgatcgaga ttgtaccagc cttggccatc agcgtttcgt
    21901 ccaagaacgt agtaccaagc atcattggta ttaaggattt ggtgaccttt ttcagctagt
    21961 agtttagaag aagcgacatc gtagcctccc caaccaccag tccacataga aacgatgatg
    22021 tctttgtcaa aactaccaaa gcttgtgtcg ctattgtagt agataccgtc gttaaaggcc
    22081 attggtttga gaccgtgcga ttttacaata cgagcgaggt cattggcgta ggcaataaat
    22141 ttttcatagc cttttacagg gtagccttcg tttggatagt atttatcagc ttgaagcaca
    22201 ctccaacctt tagcatctgt cgcatcattg gcatattcat caagtccgat gttgaagatt
    22261 tcagtctttt tcgcgaaata agcagcatac ttgtcgataa gggcttttgt aaaagcgaca
    22321 gcttgttcgt tgtcaagatc gacagtacgg gctgatttct tcccaaaata gctaaagtta
    22381 gggttttgga ttcccaattc tttcatggca ttgagaatcg catccatgtg tccaggacta
    22441 tttactgtcg gaatgagacc gataccttta tctttggcat agttaatcag atctgtcatt
    22501 tgactttctg ttaagtgatt gccgtttgga tcgttgtaat aatcatttgt acctttttca
    22561 atggcgcgtt tgacatcgtc actggcatag gtcttgccgt tagctgtgat gctcatatcg
    22621 tccaacatga aacggagtcc atcatttccg actaataggt gtaaatcagt gtagccataa
    22681 tgtttcgctt tatcgatgat ttccttgagc tgttctggtg agaaatattt acgtccagca
    22741 tcaatagaaa caattttctt tttcgctagt ttttcattta cagttgcagc acgttccttt
    22801 cctgcctctg tttccggttt gtcagcctct gctttcgctt catctttttt agctggttta
    22861 tccttgtcag tcttgtctgt atttgactct ttagaatcaa cctctttcgc ttcttccttt
    22921 ttagggctag cttcttctgc ctttttatta gcagtttctt tttcagcaga agttggagtt
    22981 accacttctg ctttatcact aggagttgaa ctaacttcct cttgtggttt ttcttctgtt
    23041 tttggaagac tagctacctt atcagtagct ggagtttctg tttctacagt ttttggagct
    23101 tctggttgaa gctctgcttt aggtgtttcc tcagtccgat tttcggatga ttgaggggaa
    23161 tcagaaaccg tatggatagt cggttggttt tctgtagtag taggagtaac tccatcggct
    23221 gcaacagtct gtgcttggaa ggcaaatcca attagaacag aagctgctcc tacagcgtat
    23281 ttacgaatag aaaaacgctg ttgtttttca tgtttcattg caaaacctcc tgattgcatt
    23341 gttatattga tagcgattat ataaatcaac gcctttattt tatttcttat attaacgaga
    23401 gtcaagagga gatgacaaaa aactataata agtataaaaa aatataaaat ttaaacttaa
    23461 gatttcagat tggtcgaaaa aatacatata tatatctagt ataatttttg gttctatttc
    23521 tataaaatat tccacaaatt atagaatctt ccaaaaatag gtaagcgcta cctttttggt
    23581 gtggtataat aagcatagaa aaagcccgag cgattaggct caggctttct tcttagtgat
    23641 cacggtcaca tgagatgaat ttaatcttgt agtaatcaga tcgtttgtaa gtttcactgt
    23701 attctaaaac ttggccagtt gattcgagtt tggtgatttt agtttgtagg acagtaggga
    23761 attgttcatc gactccgagg actgaagccg catgttctgg agttggaaag actatttcgt
    23821 tgatttcttc aaagtgttca tcattcatgt gaatgtggta gtctaacttg aaacgattat
    23881 agatagaact atagtattca aggtttggat aatttgcgtt gatatattgt tctgggatgt
    23941 aggatgtatg gtagatataa acgacaccgt ttgattcgcg gatacgttca atcttgtagt
    24001 agaattgatc gccgcgtaga cccaattttt ccaagtaaac aagcttgttt ccgcgttcaa
    24061 ttgaaagaac agttacctta tcatctttag cattgaagag ttcaatatct gaaaactcta
    24121 caagcttgtg tttgcgtgca cgtgaaacga aggttccttt tccttgttgg cggacaatat
    24181 agccatcttt ggcaaggtcg tttaaggcgc gaacaactgt gatagagctg acatcgtaca
    24241 ttgaaatgag ttctgcttca gtgtaaaatt tatctccact gctaaactgc ccagagatga
    24301 ttttattttt taattcgtct tttatgtatt gatatttggg aattgccata tttttacctc
    24361 tattttcctt ctccatagaa aattttacca taaaagcgaa aaaaatagta gtcttttgaa
    24421 taaaaaatta gaataatagt ttgaaaaatt aagcgatata gggattgctc gtattttata
    24481 aatgtattct atacaaaatt attttaagtc ctatatttcg aatttaaaga gcgtttgaaa
    24541 tgggtctaaa aatgtttgaa actaattgaa tgtgtgtata atagtaaaaa tgcagtaaat
    24601 atcccgacaa tttctattga caattcaaac agattggttt ataattaata taacaacaaa
    24661 tgaaagcgca aactttcgtg gtcggaaggt agttttatga cacgatttga gatacgagat
    24721 gatttctatc tcgatggaaa atcatttaag attttatctg gtgccattca ttattttagg
    24781 gttcctccag aggattggta tcattcgctc tataacttga aggctcttgg ttttaatacg
    24841 gtagagactt atgttgcttg gaatttacac gagcctcgtg aaggtgaatt tcattttgac
    24901 ggtgatctgg atttagagaa atttctccaa atagcgcagg atttgggtct ctacgcaatt
    24961 gtgcgtccgt ctccatttat ctgtgcggaa tgggaattcg gtggcttacc agcttggctc
    25021 ttgaccaaga acatgcgaat tcgctcatcc gacccagcat atatcgaggc agttggtcgc
    25081 tactatgatc agttattgcc aagactggtg tctcgtttgt tggacaatgg tggcaatatt
    25141 ctcatgatgc aggttgaaaa tgagtatggt tcttacggag aagataaggc ttacctgaga
    25201 gcgattcgac agctaatgga agagtgtggc gtaacctgtc ccctctttac atcagatggt
    25261 ccatggcgag ctactctgaa agctggaacc ttaattgaag aggacctctt tgtaacagga
    25321 aactttggtt ctaaggcacc ttacaacttt tcgcagatgc aggaattctt tgatgaacat
    25381 ggtaagaaat ggccactcat gtgtatggag ttctgggatg gttggttcaa tcgctggaaa
    25441 gagccgatta tcacacggga tcctaaggaa ttggcagatg cagttcgaga ggttttggaa
    25501 caaggctcta tcaatcttta catgttccac ggtggtacaa actttggttt catgaatggt
    25561 tgctcagctc gaggaacttt ggacctgcca caagttacat cttatgatta cgatgccctt
    25621 ctggatgaag aaggaaatcc aactgctaaa tatcttgcag tcaagaagat gatggcaaca
    25681 catttttcag agtatccgca gttggaacca ctctacaaag agagtatgga gttggatgct
    25741 attccactag ttgaaaaagt ttctttgttt gaaaccttag atagcttgtc aagtcctgta
    25801 gaaagtctct atcctcaaaa gatggaggag ctgggacaaa gttatggcta cctactttat
    25861 cgaacagaaa caaactggga tgcagaagaa gaaagacttc gtatcattga tggtcgagat
    25921 agggcccagc tgtatgtcga tggtcagtgg gttaaaactc aatatcagac agagattggg
    25981 gaagatattt tttatcaagg taaaaagaaa gggctatcta ggttagatat cttgatagaa
    26041 aatatggggc gtgtcaacta tgggcataag ttcttagcgg atacgcaacg taagggaatt
    26101 cggacagggg tctgtaagga tctgcatttc ttactaaact ggaaacacta tccactccca
    26161 ctagacaatc ctgagaaaat tgatttttca aaaggatgga ctcaaggaca accagccttt
    26221 tacgcttatg actttacagt cgaagagcca aaagatactt acctagactt gtctgagttt
    26281 ggtaagggag ttgcctttgt caatgggcag aatctaggac gtttttggaa cgttggccca
    26341 actctctcac tttatatccc tcatagctat ctcaaggaag gtgccaaccg tatcattatc
    26401 tttgaaacag aaggtcaata taaagaagag attcatttaa ctcgtaaacc tacactaaaa
    26461 catataaagg gggaaaactt atgacaattg taggatgccg tattgatgga cgtttgatcc
    26521 acggacaagt agccaatctt tgggctggaa aactaaatgt ttcacgcatt atggttgtag
    26581 acgacgaagt tgtcaacaac gatattgaaa agagtggttt gaaacttgcg acaccaccag
    26641 gtgtgaaatt gagtattttg ccagttgaga aagctgcagc caatattctt gctggtaaat
    26701 acgatagcca acgtctcttt atcgtggctc gtaaaccaga ccgcttcctt ggtttggtag
    26761 aagcaggtgt accacttgaa acccttaatg ttgggaatat gtctcaaaca ccagaaactc
    26821 gttctattac acgttctatc aacgtagtag acaaggatgt ggaagacttc cacaaactgg
    26881 cagaaaaagg tgttaaactt actgctcaga tggttccaaa tgatccaatt tcagactttt
    26941 tgagcttatt aaaataggaa aaaaattttt aggaggtcat tgttatgata caatggtggc
    27001 aaattttact tctcactttg tactcagctt atcaaatctg tgatgagttg acgatcgttt
    27061 catctgcagg ttcccctgta tttgctggtt tcattactgg tttaatcatg ggagatgtga
    27121 ctactggttt acttatcggt ggtaacttgc aactgttcgt tcttggggtt ggtaccttcg
    27181 gtggtgcttc tcgtatcgac gcaacttctg gtgcggttct tgcgacagcc ttctctgttt
    27241 cacaaggaat tgatgcaccg cttgccatta ctacaatcgc tgtaccagta gcagctctct
    27301 tgacttactt cgacgttctt ggtcgtatga ctactacctt cttcgctcac cgtgtggatg
    27361 ctgcaatcga acgctttgac tataaaggta ttgaacgcaa ctacttgctt ggtgcgattc
    27421 cgtgggctct atctcgtgcc cttccagtct tctttgccct tgcttttggt ggtgcctttg
    27481 tacaatcagt agtagacttc gttgaagcct acaaatgggt tgcagatggc ttgacacttg
    27541 caggacgtat gcttccaggt cttggatttg caatcttgct tcgttacctt ccagttaaac
    27601 gtaaccttca ctaccttgct atgggatttg gtttgacagc tatgttgact gttctttact
    27661 catatgtaac aggtcttggt ggcgctgttg ctggtatcgt aggtactctt cctgctgaag
    27721 ttgctgaaaa aattggtttc gtgaacaact tcaaaggttt gtctatgatt ggtatttcta
    27781 tcgtaggtat tttccttgca gtgcttcact tcaaaaatag ccaaaaagta gctgtagcag
    27841 caccttctac accatcagaa agtggggaaa tagaagatga cgaattctaa ttacaaactt
    27901 acaaaagaag attttaatca aatcaacaaa cgtagcttgt ttactttcca attaggttgg
    27961 aactacgaac gtatgcaagc ttctggttac ctttacatga tcttgcctca gttgcgtaaa
    28021 atgtatggtg atggaactcc tgaattgaaa gaaatgatga aagttcatac tcaattcttc
    28081 aatacttcgc cattcttcca taccattatc gctggttttg accttgccat ggaagaaaaa
    28141 gatggtgtag gttcaaaaga cgccgttaac ggtatcaaga caggtttgat gggaccattc
    28201 gctcctcttg gggatacaat ctttggttca cttgtacctg ctatcatggg gtcagtcgca
    28261 gcaactatgg ctatcgctgg ccaaccttgg gggatcttcc tttggattgc agttgcagta
    28321 gcgtatgaca tcttccgttg gaaacagttg gaatttgctt acaaagaagg ggttaacctt
    28381 atcaacaaca tgcaaagtac cttgacagct ttgattgacg ctgcatctgt acttggtgtc
    28441 ttcatgatgg gtgctcttgt agcaacagtg attaactttg aaatttctta caagttgcca
    28501 atcggtgaaa agatgattga tttccaagac atcttgaacc aaatcttccc acgtttgctt
    28561 ccagcaatct ttactgcctt tatcttctgg ttgcttggta agaaaggtat gaactctact
    28621 aaagctatcg gtattattat cgtacttgct ttggctcttt ctgcccttgg tcactttgca
    28681 cttggaatgt aattccttat gactaaatca ttaattttgg tgagccatgg tcgcttctgt
    28741 gaggagctta gaggtagcac agaaatgatt atgggcccac aagacaacat ttacacagta
    28801 gctcttcttc cagaagatgg cccagaagaa tttactgcta aatttgaagc tgttattgaa
    28861 gggttggatg atttcctagt ctttgcggat cttctcggtg ggacaccttg taatatggtg
    28921 agtcgcttga tcatggaagg tcgtgatatt gacctttacg cagggatgaa tcttccaatg
    28981 gtgattgaat ttatcaatgc gagccttaca ggcgcagatg cggactacaa gatccgtgct
    29041 gcagaaagca ttgtgaaagt taatgacctg ttagcgagct tcgatgatga cgaagatgaa
    29101 taatactctt cgaaaaactc ttcaaactac gtcaacgtcg ccttgccgta ggtatatgtt
    29161 actgacttcg tcagtcttat ccggcaacct caaaacggtg ttttgagctg acttcgtcag
    29221 ttctatctgc aacctcaaag cagtgctttg agcagcctgc ggctagtttc ctattttagt
    29281 tggaactcga ttcaattcat gtgacaacgt gaaaatcgtt agagcatttt atatagaata
    29341 tacatgggaa tgtagcttac tctcattccc atatttaata gaaaaagagg aactcaatgc
    29401 tacattatac aaaagaagac ttgctcgaat tgggtgcaga aatcactacg cgtgaaatct
    29461 accaacagcc tgatgtatgg agagaagctt ttgaatttta tcaagcaaaa cgtgaagaaa
    29521 ttgcagcctt cctacaagaa atcgctgata aacatgacta tattaaggtt atcttgacag
    29581 gtgctgggac ttctgcttat gtgggagata ccttgctacc ttattttaag gaagtctatg
    29641 acgaacgcaa atggaatttc aatgctattg cgacaacaga tatcgttgcc aatccagcaa
    29701 cctatttgaa aaaagatgtg gcaactgtcc ttgtgtcttt tgctcgtagt gggaactcgc
    29761 ctgaaagtgt ggcgactgtt gatttggcca aatccttggt ggatgagctt tatcaagtga
    29821 cgattacttg tgcagcagat ggtaaattgg ctcttcaagc tcacggtgat gaccgtaatc
    29881 tcttgctctt gcaaccagct gtctctaatg atgctggatt tgccatgact tctagcttta
    29941 cgtctatgat gttgacagct ctcttggtct ttgatcctac agaatttgct gttaagtctg
    30001 aacgttttga agttgtatct agtcttgccc gtaaagtttt agacaaggca gaagatgtca
    30061 aagagctcgt tgatttagac tttaaccgtg tcatctatct aggcgctggt cctttctttg
    30121 gacttgctca tgaagctcag ctcaagattt tggaattaac tgctggtcaa gttgcgacca
    30181 tgtatgaaag cccagttggc ttccgtcacg gtccaaaatc tcttatcaac gacaatacag
    30241 ttgttttggt ctttggtaca acgacagact acactcgtaa gtacgacttg gacttggttc
    30301 gtgaagttgc tggtgaccag attgctcgtc gtgttgtgct tttgagtgat caagcttttg
    30361 gtcttgaaaa tgtcaaagaa gtggcccttg gttgtggcgg tgtcttgaat gatatttacc
    30421 gtgtcttccc ttacatcgtt tatgcccaac tctttgccct attgacttca ctcaaggtag
    30481 aaaataaacc agatacaccg tctcctacag gtacagtaaa ccgtgtagta caaggtgtca
    30541 taattcacga atatcaaaag taagacagtg tttatgaatt cttgataaga ggatttgtaa
    30601 attatcagat aaaccataga ttgtcagtat gctttctatg gtttgtttgc ttgagagaaa
    30661 tagtaaaagg agaacagaat gaaagcatac acagagcgtg tatttggaaa tgttgagggc
    30721 gaggatgtct tggcctatcg atttgagaca gacggtggct accaacttga ggttatgact
    30781 tatggcgcga ccatcttgcg ctatgtcgca cctgacaagg ctggaaattt tgccaatgtt
    30841 atcttgggat ttgatgactt tgatagttat gtaggcaata gtcccaagca tggagcaagt
    30901 gtaggtcctg tagcgggtcg tattgcaggt gcgacctttg agctcaatgg taagacctat
    30961 gaccttgagg ttaataatgc tagcaactgt aatcacagtg gttcaactgg ttgggattcc
    31021 agcttgtttg aagttgaaga agtaagcgat catggcttga ctctctacac agagcgtaca
    31081 gatgggacag gagggttccc tggaaatctc aagatttgga tcagttatca cttggaagaa
    31141 actggtgcct atgaaatcag ctacaaggta acgaccgatc aggatacgct ggtcaatcca
    31201 accaaccaca gctatttcaa cttgtctggt gatttcacgc agacgattga ccgtcatgtc
    31261 ttccaactaa acacagaggg catttactca atcgctcctg acggtgttcc tgccaaaact
    31321 ccagaagcca accgtgatgt ggtcaaacac atctacaatg gtaccttgtt gaaggatatc
    31381 tttgcagaag aagatgagca aatccagctg gcatcaggtt tggatcaccc atttgccctt
    31441 cctgtaggcc atgacaatgc tggattcctt tatgaccaaa attcaggtcg cttcctgctt
    31501 ttcaagacag aagctccttg ctttgtggtc tacacagcaa actttgtgga tgaaagtgtc
    31561 atcataggag gtcagccaat gctacagcac aatgggattg ctcttgaagc acaagcttta
    31621 ccagatgcca ttcacagtga ccttaaaggc caagtcattc ttaaagctgg tcaaaccttc
    31681 accagtaaga cacgttatga acttgttgtg aaataaaaaa agctggtcaa aaagtcttta
    31741 aaattaaaat aatgcatatt gtcaggtgat ggaaagtttg ataatatgcg ttttattata
    31801 aaatgattta ctttatctta tcctgaaatg gtgttatttt tgagttatct ggcttgactt
    31861 tcttgatgaa aattatataa tagttgtaat aataataatt acaacgagaa aagaggacca
    31921 tttatgacct atgaatacaa gagtcacatt tatttggcag agacagcttt aaatgtaaag
    31981 gatttggcca gtcaaacagc cttttatcag caagttattg gtttagaaat cttatctcaa
    32041 actgagacag agtccattct aggccttggt ggaaaagtct tggtacagct gattcaagca
    32101 caagagagcg gagaagtgag ggaacattat ggtctttacc atttggccat tctcttgccc
    32161 acacgcaagg ctttggctga tgtcttgaaa cacctgacag atttacagat tcctcttgtc
    32221 ggcggtgcag accatggtta cagtgaagct atttacttgg aagatttgga gggaaatggc
    32281 attgaactct atcgagataa gccagtttcc acatgggata ttcgagaaga tggacgcatt
    32341 atcggagtga cagaggctct tgcggcgcag gacatttatg agttggggga aagagtagag
    32401 ccctttatcc tggcagaagg tacgagaatg gggcatattc atctttccgt caaggatagt
    32461 cgaaagtcca gccagtttta tcaaaaggtg ttagggctag aggataaatt cagtgtgcct
    32521 agcgctagtt ggattgcggc tggagattac catcatcatt tagcagttaa cgaatgggga
    32581 ggaaaaggtc tggcttcgcg taagcaaggc ttaccaggtt tagcctacta tgtcatcgaa
    32641 gtcgcacata aaaaagaact attaacgatt gcccaacgag cacaagaagt tgacgcacca
    32701 atcaaatgga tgacatcgag ccaattggaa atcacagact cagatggcat cgtgacccgt
    32761 attcgtttag caaggtaaaa ttaggaattt ttaataagtt ataagtattg ctagtttgct
    32821 ttttgaacgg tctttggtaa cagaactgcg tgagctggat gggcgagaat tttgacaaag
    32881 aagctgatta gagatataat gaagaaaaat cgagcaagga aaaagaaatg acaaattcaa
    32941 agtatattac ttgtttgaaa cgttcagagg gccagttgtg tgggattcaa aagatgattg
    33001 aaggagatcg tgactgtgct gatattgtga cgcaactgac agcagtgaga tctagtgtag
    33061 agcgcgtgat tgagatgata attaccgaaa atcttactga atgtatcaat cagccactag
    33121 atgattctga agctcaaaag gaacgcctag aaaaggctat ccgatacttg attaaacgga
    33181 aataaatgaa tgttttgaga cgatatgtgc aaaggtctat taactattag aatagtttaa
    33241 tgtgagtcaa aagaagttgt aaaaaatctt taaaattaaa aaaacgcata gtatcaggtg
    33301 ttgaatgtac tgaccccaaa aagttagatt ttttctgtct aacttttggg gtgcagttca
    33361 taagaacctt ggtaatatgc gttttattat gtagagactt atactcttcg aaaatctctt
    33421 caaaccacgt cagtttcacc ttgccgtaga tatggttact gacttcgtca gttctatcta
    33481 caacctcaaa acagtgtttt gagctgactt cgtcagttcc atccacaacc ttaaaacagt
    33541 gttttgagca gcctgcggct aacttcctag tttgcacttt aattttcatt gagtattact
    33601 tcatcttctc ctgaaattga gtttttgccc agctcctctt gattattcta cttgaccggg
    33661 ccaagcattc ataccacctt ctacgttggt aacggtgagg ccttgggagc tgagaaattg
    33721 gcaggtagat gcagagcgga ctcctccttg acaaataaca tagtattcat ggtcaggttt
    33781 gagttctttg tagccttgct ctaaggtact taatggaaga tttttggcat ctggtgcatg
    33841 tcctgcttgg aattcatgtg cttcacggac atcgataagt tctagatttt cattttgata
    33901 ttttccataa aagtcagcca tgctgatact agtttccata ttctactcct tctgggttag
    33961 tcattttgta tagtgaataa gcgccgtcaa ggttttggac ggtaaatcct gcttgcttga
    34021 ggataggctc tgcgatatag ctgcgcaaac cactgtggca gctgacgatg taggcttgtt
    34081 tttctcctca gttcatcagc ttcctccttg acactttgtc agattttgat acaatagtac
    34141 aaaattagag gaggcaggct atgattcaga aacatgcgat tcctatttta gagtttgatg
    34201 acaatcctca ggcggttatc atgcccaatc acgaggggct ggacttgcag ttgccaaaga
    34261 agtgtgttta tgcattttta ggtgaggaga ttgaccgcta tgcaagggaa gtaggggcgg
    34321 attgtgtcgg cgaattcgtt tctgctacca agacctatcc agtctatgtc atcaactaca
    34381 agggtgagga ggtctgtctg gctcaggctc ctgttggctc cgctccagca gcccagttta
    34441 tggattggtt gattggctat ggtgtggagc agattatctc tactgggacc tgtggtgtgc
    34501 tagccgatat agaggaaaat gcctttctag tccctgttcg cgctctgcga gatgagggag
    34561 ccagttacca ctatgtggca ccttgtcgtt atatggaaat gcagccagag gctattgctg
    34621 ctattgagga agttttggaa gacagaggga ttccttatga agaagtcatg acctggacga
    34681 cagacggttt ttaccgagaa acggctgaaa aggtggctta tcgtaaggaa gaaggctgtg
    34741 ctgttgtgga gatggagtgt tctgctcttg cggcagtagc tcaattgcgt ggggttctct
    34801 ggggtgaatt gttgttcaca gcagattctc tagcggactt ggaccagtac gacagtcgtg
    34861 actggggctc ggaagctttt aataaggcgc tagaactgag tttagcaagt gttcaccacc
    34921 tttagttgta ctggcaaagg atttgtttta tcataaaatg tctagctcat acttttcaaa
    34981 aatatgttta aacgaggtca ccttcctctt gtcctaggca tgttgaggtt gggaaaaatc
    35041 tttaaaatca gaaaaacgta tcatatcagg tgatgaaaac tttgatacta tgcgttttat
    35101 gttggtaaga tttagagtga gatgaaatga tactcttcga aaatcttttc aaaccaggtc
    35161 agcttcacct tgccgtatag atgttactga cttcgtcagt tctatctgca acctcaaaac
    35221 agtgttttga gcaacctgtg actagctttc taagcgatgc cttggttctc attgcctata
    35281 atcaaaaaga gaaattttct cctgaaaagc atatagagta gctggcgtta aaagctcctg
    35341 tcttgctttt ttgacctata gtcacatcta tcaagtattg ttcttgccta agctatcaat
    35401 aaaaaggtgg cattttttag gcttggtgtt agtgaatttt gccttatcct atctaagtca
    35461 tttcgagctt tttatggtac aatggaaaca tgttattcaa attatctaag gaaaaaatag
    35521 agctaggctt atctcgttta tcgccagccc gtcgtatttt tttgagtttt gccttggtca
    35581 ttttactagg ctctcttctt ttgagcttgc cctttgtcca agttgaaagc tcacgagcga
    35641 cttattttga tcatcttttc actgctgtct ctgcagtctg tgtgacaggt ctctcaaccc
    35701 ttccagtagc tcacacctat aatatatggg gccaaataat ctgtttgctc ttgattcaga
    35761 tcggtggtct agggctcatg acctttattg gggttttcta tatccagagc aagcaaaagc
    35821 ttagtcttcg tagccgtgca actattcagg atagttttag ttatggagaa actcgatctt
    35881 tgagaaagtt tgtctattct atttttctca cgaccttttt ggttgagagc ttgggagcta
    35941 ttttgcttag ttttcgcctt attcctcaac ttggctgggg acgtggtctt tttagttcca
    36001 tttttctagc gatctcagcc ttctgtaatg ccggttttga taatttaggg agcaccagtt
    36061 tatttgcttt tcagaccgat ttactggtca atctggtgat tgcaggcttg attattacag
    36121 gcggccttgg ttttatggtc tggtttgatt tggctggtca tgtaggaaga aagaaaaaag
    36181 gacgtctgca ctttcatacg aagcttgtat tattattgac tataggtttg ttgttatttg
    36241 gaacggcaac tactctcttt cttgagtgga acaatgctgg aacgattggc aatctccctg
    36301 ttgccgataa ggttttagtt agcttttttc aaacagtgac gatgcgaaca gctggctttt
    36361 ctacgataga ttatactcag gctcatcctg tgactctttt gatttatatc ttacagatgt
    36421 ttctaggtgg ggcacctgga ggaacagctg ggggactcaa gattacgaca ttttttgtcc
    36481 tcttggtctt tgcacgaagt gagcttctag gcttgcctca tgccaatgtt gcgagacgaa
    36541 cgattgcgcc gcgaacggtt caaaaatcct ttagtgtctt tattatcttt ttgatgagct
    36601 tcttgatagg attgattctg ctagggataa cagccaaagg caatcctccc tttatccacc
    36661 tcatatttga aactatttca gctcttagta cagttggtgt aacggcaaat ctgactcctg
    36721 accttgggaa attggctctc agtgttatca tgccacttat gtttatggga cgaattggtc
    36781 ccttgacctt gtttgttagc ttggcagatt accatccaga aaagaaagat atgattcact
    36841 atatgaaagc agatattagt attggttaag aaaggaaaga gcatgtcaga tcgtacgatt
    36901 ggaattttgg gcttgggaat ttttgggagc agtgtcctag ctgccctagc caagcaggat
    36961 atgaatatta tcgctattga tgaccacgca gagcgcatca atcagtttga gccagttttg
    37021 gcgcgtggag tgattggtga catcacagat gaagaattat tgagatcagc agggattgat
    37081 acctgcgata ccgttgtagt cgcgacaggt gaaaatctgg agtcgagtgt gcttgcggtt
    37141 atgcactgta agagtttggg ggtaccgact gttattgcta aggtcaaaag tcagaccgct
    37201 aagaaagtgc tagaaaagat tggagctgac tcggttatct cgccagagta tgaaatgggg
    37261 cagtctctag cacagaccat tcttttccat aatagtgttg atgtctttca gttggataaa
    37321 aatgtgtcta tcgtggagat gaaaattcct cagtcttggg caggtcaaag tctgagtaaa
    37381 ttagacctcc gtggcaaata caatctgaat attttgggtt tccgagagca ggaaaattcc
    37441 ccattggatg ttgaatttgg accagatgac ctcttgaaag cagataccta tattttggca
    37501 gtcatcaaca accagtattt ggatacccta gtagcattga attcgtaaag agggatgacc
    37561 cctctttttt gatgcccaag atggcaaata gagacagaag ccccttgtct tctagtaaaa
    37621 gttcttcaaa ggctggactt tatggtaaaa tagaaggaag tgacaagaga gagtaatact
    37681 caatgaaaat caaagatcaa actaggaaac tagctgcggg ctgctcaaaa cactgttttg
    37741 aggttgtgga tagaactgac gaagtcagct caaaacactg ttttgaggtt gcagatagaa
    37801 ctgacgaagt cagtaacatc tatacggcaa ggcgacgttg acgcggtttg aagagatttt
    37861 cgaagagtat aagaaaaaat cagtccccta aaggagcaga ttatgaagtt attgtctatc
    37921 gccatttcta gctataatgc agcagcctat cttcattact gtgtggagtc gctagtgatt
    37981 ggtggtgagc aagttgggat tttgattatc aatgacgggt ctcaggatca gactcaggaa
    38041 atcgctgagt gtttagctag caagtatcct aatatcgtta gagccatcta tcaggaaaat
    38101 aaaggccatg gcggtgcggt caatcgtggc ttggcagagg cttctgggcg ctattttaaa
    38161 gtagttgaca gtgatgactg ggtggatcct cgtgcctact tgaaaattct tgaaaccttg
    38221 caggaatttg agagcaaggg tcaagaggtg gatgtctttg tgaccaattt tgtctatgaa
    38281 aaggaaggtc agtcttgtaa gaagagtatg agttacgatt cagtcttgcc tgttcggcag
    38341 atttttggct gggaccaggt cggaaatttc tccaaaggcc agtatatcat gatgcactcg
    38401 ctgatttatc ggacagattt gttgcgtgct agccagttct aattgcctga acatactttt
    38461 tatgtcgata atctctttgt ctttacgccc cttcagcagg tcaagaccat gtactatctg
    38521 cctgtcgatt tctatcgtta tttgattggg cgtgaggacc agtctgtcaa tgagcaagtg
    38581 atgattaagt gcattgacca gcaactcaag gtcaatcgac tcttggtaga ccaacttgat
    38641 ttgtcccaag tgagtcatcc caaaatgcga gaatatctgc tgaatcatat tgaaatcacg
    38701 acggtgattt ccagtaccct gctcaaccga tctggaacag cggagcatct ggcaaaaaaa
    38761 cgccaattgt ggacctatat tcagcagaaa aatccagaag tctttcaggc tattcgtaag
    38821 accatgttga gccgtttgac caaacattct gtcttgccag atcgcaaact gtccaatgtc
    38881 gtctatcaaa tcaccaaatc tgtttatgga tttaattaat ataagtgttt tataagaggg
    38941 atttaagaaa aattttaact ttttcttagt cctttttaat ttcaggagat tatactagag
    39001 tcatcaaata aagaaagact ctaaggagaa tcctatgaaa ttcaatccaa atcaaagata
    39061 tactcgttgg tctattcgcc gtctcagtgt cggtgttgcc tcagttgttg tggctagtgg
    39121 cttctttgtc ctagttggtc agccaagttc tgtacgtgcc gatgtggtca atccgacccc
    39181 aggtcaagtc ttacctgaag agacatcggg aacgaaagag ggtgacttat cagaaaaacc
    39241 aggagacacc gttctcactc aagcgaaacc tgagggcgtt actggaaata cgaattcact
    39301 tccgacacct acagaaagaa ctgaagtgag cgaggaaaca aactcttcta gtctggatac
    39361 actttttgaa aaagatgaag aagctcaaaa aaatccagag ctaacagatg tcttaaaaga
    39421 aactgtagat acagctgatg tggatgggac acaagcaagt ccagcagaaa ctactcctga
    39481 acaagtaaaa ggtggagtga aagaaaatac aaaagacagc atcgatgttc ctgctgctta
    39541 tcttgaaaaa gctgaaggga aaggtccttt cactgccggt gtaaaccaag taattcctta
    39601 tgaactattc gctggtgatg gtatgttaac tcgtctatta ctaaaagctt cggataatgc
    39661 tccttggtct gacaatggta ctgctaaaaa tcctgcttta cctcctcttg aaggattaac
    39721 aaaagggaaa tacttctatg aagtagactt aaatggcaat actgttggta aacaaggtca
    39781 agctttaatt gatcaacttc gcgctaatgg tactcaaact tataaagcta ctgttaaagt
    39841 ttacggaaat aaagacggta aagctgactt gactaatcta gttgctacta aaaatgtaga
    39901 catcaacatc aatggattag ttgctaaaga aactgtcgaa aaagctgtta aagataacgt
    39961 taaagacagt atcgatgttc cagcagccta cttagaaaaa gccaagggtg aaggtccatt
    40021 cacagcaggt gtcaaccatg tgattccata cgaactcttc gcaggtgatg gcatgttgac
    40081 tcgtctcttg ctcaaggcat ctgacaaggc accatggtca gataacggtg acgctaaaaa
    40141 cccagcccta tctccactag g
//
LOCUS       DAVKQL010000013        38244 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVKQL010000013 DAVKQL010000000
VERSION     DAVKQL010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 38244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38244
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.013"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            2..74
                     /locus_tag="VW561_000584"
     tRNA            2..74
                     /locus_tag="VW561_000584"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            99..171
                     /locus_tag="VW561_000585"
     tRNA            99..171
                     /locus_tag="VW561_000585"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:132..134,aa:Lys,seq:ttt)
     gene            177..258
                     /locus_tag="VW561_000586"
     tRNA            177..258
                     /locus_tag="VW561_000586"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:211..213,aa:Leu,seq:tag)
     gene            277..349
                     /locus_tag="VW561_000587"
     tRNA            277..349
                     /locus_tag="VW561_000587"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:310..312,aa:Thr,seq:tgt)
     gene            372..443
                     /locus_tag="VW561_000588"
     tRNA            372..443
                     /locus_tag="VW561_000588"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:404..406,aa:Gly,seq:gcc)
     gene            451..536
                     /locus_tag="VW561_000589"
     tRNA            451..536
                     /locus_tag="VW561_000589"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:485..487,aa:Leu,seq:taa)
     gene            546..619
                     /locus_tag="VW561_000590"
     tRNA            546..619
                     /locus_tag="VW561_000590"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:580..582,aa:Arg,seq:acg)
     gene            635..708
                     /locus_tag="VW561_000591"
     tRNA            635..708
                     /locus_tag="VW561_000591"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:669..671,aa:Pro,seq:tgg)
     gene            714..787
                     /locus_tag="VW561_000592"
     tRNA            714..787
                     /locus_tag="VW561_000592"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:748..750,aa:Met,seq:cat)
     gene            801..874
                     /locus_tag="VW561_000593"
     tRNA            801..874
                     /locus_tag="VW561_000593"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:835..837,aa:Ile,seq:cat)
     gene            885..974
                     /locus_tag="VW561_000594"
     tRNA            885..974
                     /locus_tag="VW561_000594"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:921..923,aa:Ser,seq:tga)
     gene            988..1061
                     /locus_tag="VW561_000595"
     tRNA            988..1061
                     /locus_tag="VW561_000595"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1022..1024,aa:Met,seq:cat)
     gene            1065..1137
                     /locus_tag="VW561_000596"
     tRNA            1065..1137
                     /locus_tag="VW561_000596"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1098..1100,aa:Phe,seq:gaa)
     gene            1157..1227
                     /locus_tag="VW561_000597"
     tRNA            1157..1227
                     /locus_tag="VW561_000597"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1189..1191,aa:Gly,seq:tcc)
     gene            1263..1336
                     /locus_tag="VW561_000598"
     tRNA            1263..1336
                     /locus_tag="VW561_000598"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1297..1299,aa:Ile,seq:gat)
     gene            1343..1430
                     /locus_tag="VW561_000599"
     tRNA            1343..1430
                     /locus_tag="VW561_000599"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1377..1379,aa:Ser,seq:gct)
     gene            complement(1556..2491)
                     /locus_tag="VW561_000600"
     CDS             complement(1556..2491)
                     /locus_tag="VW561_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEU9942898.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLIQCDNGKEIWLNSGEISLNSWK"
     gene            complement(2488..3348)
                     /locus_tag="VW561_000601"
     CDS             complement(2488..3348)
                     /locus_tag="VW561_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU9942899.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELSQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYIRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            3456..5618
                     /locus_tag="VW561_000602"
     CDS             3456..5618
                     /locus_tag="VW561_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEU9942900.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSC
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQIEKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRRHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHDIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMMRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGLVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTVSNEAAVQFNYENQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEIYSGAELMYAGLTVILSQGDFLSRQYIFRRL"
     gene            5653..6912
                     /locus_tag="VW561_000603"
     CDS             5653..6912
                     /locus_tag="VW561_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9942901.1"
                     /translation="MEWYKKIGLLATTGLALVGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            6926..7792
                     /locus_tag="VW561_000604"
     CDS             6926..7792
                     /locus_tag="VW561_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9942902.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSMVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            7806..8639
                     /locus_tag="VW561_000605"
     CDS             7806..8639
                     /locus_tag="VW561_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9942903.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     LFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQCHIISGMSNGAV
                     K"
     gene            8950..10392
                     /gene="gtfA"
                     /locus_tag="VW561_000606"
     CDS             8950..10392
                     /gene="gtfA"
                     /locus_tag="VW561_000606"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEU9942904.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGERYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFTLPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVVEIDLQNQTYRVIENGVEVSF"
     gene            10530..11723
                     /locus_tag="VW561_000607"
     CDS             10530..11723
                     /locus_tag="VW561_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU9942905.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            12409..14388
                     /locus_tag="VW561_000608"
     CDS             12409..14388
                     /locus_tag="VW561_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9942906.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            14455..15951
                     /locus_tag="VW561_000609"
     CDS             14455..15951
                     /locus_tag="VW561_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9942907.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGTLTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPVTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            15951..16877
                     /locus_tag="VW561_000610"
     CDS             15951..16877
                     /locus_tag="VW561_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9942908.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            16886..17953
                     /locus_tag="VW561_000611"
     CDS             16886..17953
                     /locus_tag="VW561_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9942909.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            17964..18890
                     /locus_tag="VW561_000612"
     CDS             17964..18890
                     /locus_tag="VW561_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9942910.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            19509..20318
                     /locus_tag="VW561_000613"
                     /pseudo
     CDS             19509..20318
                     /locus_tag="VW561_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813348.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            20330..21207
                     /locus_tag="VW561_000614"
                     /pseudo
     CDS             20330..21207
                     /locus_tag="VW561_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            21220..22023
                     /locus_tag="VW561_000615"
     CDS             21220..22023
                     /locus_tag="VW561_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9942911.1"
                     /translation="MASLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFISVICGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKHLNVRKRANDIARVFQDPKMGTASRLTI
                     EENMAIALRCEQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMAALVKPKLLLLDEHTAALDLKTSQMVMNLTQKIVEHHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(22056..22766)
                     /gene="treR"
                     /locus_tag="VW561_000616"
     CDS             complement(22056..22766)
                     /gene="treR"
                     /locus_tag="VW561_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEU9942912.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            22951..24918
                     /gene="treP"
                     /locus_tag="VW561_000617"
     CDS             22951..24918
                     /gene="treP"
                     /locus_tag="VW561_000617"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEU9942913.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLRDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEATKSAAKSNQNVV
                     QGVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKIEGDTNLQAEFVAQEEAEFVSHEPVELTSVEII
                     SPLTGQVKELSQATDPVFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVIVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATITGTYPTTIQAGASLMVATRI"
     gene            25096..26721
                     /gene="treC"
                     /locus_tag="VW561_000618"
     CDS             25096..26721
                     /gene="treC"
                     /locus_tag="VW561_000618"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEU9942914.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNPNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKDINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            26977..27225
                     /locus_tag="VW561_000619"
     CDS             26977..27225
                     /locus_tag="VW561_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEU9942915.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            27432..28226
                     /gene="racE"
                     /locus_tag="VW561_000620"
     CDS             27432..28226
                     /gene="racE"
                     /locus_tag="VW561_000620"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEU9942916.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            28223..29194
                     /locus_tag="VW561_000621"
     CDS             28223..29194
                     /locus_tag="VW561_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEU9942917.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKLSL"
     gene            29170..29691
                     /locus_tag="VW561_000622"
     CDS             29170..29691
                     /locus_tag="VW561_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU9942918.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPY
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWMEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            29688..30149
                     /locus_tag="VW561_000623"
     CDS             29688..30149
                     /locus_tag="VW561_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9942919.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            30140..30880
                     /gene="xerD"
                     /locus_tag="VW561_000624"
     CDS             30140..30880
                     /gene="xerD"
                     /locus_tag="VW561_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEU9942920.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            30880..31608
                     /locus_tag="VW561_000625"
     CDS             30880..31608
                     /locus_tag="VW561_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEU9942921.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLVGRAQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            31627..32196
                     /locus_tag="VW561_000626"
     CDS             31627..32196
                     /locus_tag="VW561_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEU9942922.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            32183..32905
                     /locus_tag="VW561_000627"
     CDS             32183..32905
                     /locus_tag="VW561_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU9942923.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            32905..33147
                     /gene="yidD"
                     /locus_tag="VW561_000628"
     CDS             32905..33147
                     /gene="yidD"
                     /locus_tag="VW561_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEU9942924.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(33372..34337)
                     /locus_tag="VW561_000629"
     CDS             complement(33372..34337)
                     /locus_tag="VW561_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9942925.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEITI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMADFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(34433..35185)
                     /locus_tag="VW561_000630"
     CDS             complement(34433..35185)
                     /locus_tag="VW561_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9942926.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            complement(35182..36138)
                     /locus_tag="VW561_000631"
     CDS             complement(35182..36138)
                     /locus_tag="VW561_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEU9942927.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSFFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRARQ"
     gene            complement(36128..37087)
                     /locus_tag="VW561_000632"
     CDS             complement(36128..37087)
                     /locus_tag="VW561_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9942928.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            complement(37510..38211)
                     /gene="galE"
                     /locus_tag="VW561_000633"
     CDS             complement(37510..38211)
                     /gene="galE"
                     /locus_tag="VW561_000633"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9942929.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYQVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNASGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
BASE COUNT        10949 a         7172 c         8580 g        11543 t
ORIGIN      
        1 aggtcccgta gtgtagcggt tatcacgtcg ccctgtcacg gcgaagatcg cgggttcgat
       61 tcccgtcggg accgtttaag gtaacgcaag ttattttaga ctcgttagct cagttggtag
      121 agcaattgac ttttaatcaa tgggtcactg gttcgagccc agtacgggtc atatatgcgg
      181 gtttggcgga attggcagac gcaccagatt taggatctgg cgcttaacgg cgtgggggtt
      241 caagtccctt aacccgcatt aagatataac aaatgagccg gcttagctca gttggtagag
      301 catctgattt gtaatcagag ggtcgcgtgt tcaagtcatg tagccggcat ttttagatag
      361 aagaaaacag tgcgaacgta gttcagtggt agaacaccac cttgccaagg tgggggtcgc
      421 gggttcgaat cccgtcgttc gcttagagag gccggggtgg cggaactggc agacgcacag
      481 gacttaaaat cctgcgattg gtaacgatcg taccggttcg attccggtcc tcggcataat
      541 atggagcacc cttagctcaa ctggatagag tacctgacta cgaatcaggc ggttagaggt
      601 tcgactcctc tagggtgcat ttttctattt aactcgggaa gtagctcagc ttggtagagt
      661 acttggtttg ggaccaaggt gtcgcaggtt cgaatcctgt cttcccgatt catggcggtg
      721 tagctcagct ggctagagcg tccggttcat acccgggagg tcgggggttc gatccccttc
      781 gccgctataa tgatcttgtc ggacctttag ctcagctggt tagagctctc ggctcataac
      841 cgagtggtcg taggttcaag tcctacaagg tccatttgaa tattggagga ttacccaagt
      901 ccggctgaag ggaacggtct tgaaaaccgt caggcgtgta aaagcgtgcg tgggttcgaa
      961 tcccacatcc tccttttata ttattaacgc gggatggagc agctcggtag ctcgtcgggc
     1021 tcataacccg aaggtcgtag gttcaaatcc tgctcccgca ataaggctcg gtagctcagt
     1081 tggtagagca atggattgaa gctccatgtg tcggcggttc gattccgtct cgcgccattt
     1141 tattgatagt atttatgcgg gtgtagttta gtggtaaaac tacagccttc caagctgttg
     1201 tcgcgagttc gattctcgtc acccgctttg aactttgttc aaattaccaa gttttttaac
     1261 ttgggcgcgt agctcaggtg gttagagcgc acgcctgata agcgtgaggt cggtggttcg
     1321 agtccactcg tgcccattaa ttggagaatt actcaagagg ctgaagagga cggtttgcta
     1381 aatcgttagg tcgggtaact ggcgcaaggg ttcgaatccc ttattctccg ttttaatgag
     1441 agtttgtaac tctctttttt gtttttagag acaataaaac ttcagatccg taaaacttaa
     1501 ttgaattact aatctgaagt tatttttata tcgtttatat tataattgtg ttattttact
     1561 tccaactatt gagagaaatt tcgccactat ttagccagat ttcttttccg ttatcacatt
     1621 gaattaaaag ttttccattt tctgagatgt ctttagcaag tcccttgtag tctttttgct
     1681 ctagtgtgaa agtgacttct tttcctagaa tgaatgactg ttttttgtat aggtataata
     1741 gctcttctgc tggtgtttcg aagaaagcac gccagatttc tatgatcaat tcattccttg
     1801 ttataggagc tgtagcttta aataagctgg cagctttttc ttttaattcc tgagggaagt
     1861 ctttaatagt gaaattgata cctactccaa taatgatatc tgtgactaag ccagtttcta
     1921 cagaggtcat tgcttcagta aggattcctc caattttatg attgtttaga tagatatcat
     1981 tgacccattt tatgtcgaca tctattaaag ttaggttctt aatagctttg tagacagctc
     2041 cagctacaag tagtgtgtag gatggtaatt tgtcataggg gagatttggt ttaagatgga
     2101 gtgtcatata aataccacct tgtggtgagt agaaggaacg ttgaaaacgg cctcggcctg
     2161 ctgtttgata ggaagctaga tagagggtat ttgcttcatg gcctaaatca attgcttctt
     2221 ttgcatctag ttgtgttgat tttgtttcgg gtttaaagct gactttaatt ggaagatttt
     2281 cttctagaat ctctggaaga ataaggtcac cattcatcaa tttatatcct ctatttttga
     2341 tactatcaat ttcaatgcct tcttgttcta gtcgcttgat ggctttccaa attgatgttc
     2401 ggcttaggga tagtttttct gcgatttttt ctccgctgat atagtcggtt tctttagata
     2461 ggatttggta gacagcttgg taggatttca taatgttgcc tttctcacta gttggtattg
     2521 agagtattct tttcttgtat gacttggagt ctgattaaag tattgtttat aagctttcga
     2581 aaaatggagt ggatctgaaa aacctaccga gtatgcaatt accttgatgg actcttgggt
     2641 attttcgaga agttgtctag ctcggtgcat tcgaatgtag agtaggtatt ctttgggtga
     2701 taaggtatta aattctttga atacgcttga taagtagctt ctgtgaacgg atagttcttt
     2761 tgctaaatct tgaattgtaa gtgattgagg atagtggcta tcaattaatc gtttgcattc
     2821 aagatagagt tggtgggttg atgaaatatt cttttttttc tgattgggag caatagttcc
     2881 cagatgaaac atcagttcat gaagttgtcc catgatatgg agttgagata attcacttga
     2941 ttttgtaatc tgagcgaagc ggacaatgtc tgagatgagt tttgcagtag tctgggtatg
     3001 acaagtttca gattggatga gataggattg atcagaaatt tgagaaagag caaaataatc
     3061 aggggatttc cctccagtga ttcctaacca gtagtaggcc caaggttctt tactatctgc
     3121 ttgataaaag gttagttcct ctggttttaa tagaaagaaa tctccttctt ttaaatcaac
     3181 aattttaccc ttgtaatgaa attttccttg tcctttagta atgtaatgta ggacgtatgt
     3241 atcacgaatg gctggaccaa aagagtaatt aggtgtgcat tcctcatatc cataaaagct
     3301 tagggcaagg tcaattgttc cagtctggta ttctgaaaaa actagcatgt gctacctcct
     3361 acctaacatt ttaccatatt cttgagacat ttttctattt tggaaagcga tttcaggtga
     3421 taaaatataa tcaataaagt gatgaggtga agtaaatggg agttaggata gagaataatc
     3481 tattttatgt tgagagtaaa aatctaagtt tgattattga aaatcgaaat ggctacttac
     3541 ttttgaaaca tttaggaaag actattaaga actataaagg ttccaatagt gtttatgaac
     3601 gagaccatgc cttttcagga aatccaacgg ctactaatcg aacctttagt ttagatactc
     3661 agcgacagat ttttggacaa catggcttag gagattttag gaaaccaacc atacaggttc
     3721 agcatagtgt aactgaagta acagactttc gatttgtaga agcaaagatt ttaaaaggtc
     3781 agaatggtcc acagggctta ccttctccac atagcatgga cgatacagag actcttgtct
     3841 taatgttaga agattctaag gctcaactta gtctgacttt gtattatact acttttaata
     3901 atgatgcgac tattgctagc tacagtaaat tagataataa tagtaatcag gaagttgtca
     3961 tccataaaga tttttctttt atggctgatt ttccagctgc agattacgaa atagtaactc
     4021 tgcagggtgc ttatgctcgt gaaaagactg ttagacgtca acaggtagaa caaggaatct
     4081 tttcgattag ctcaaaccga ggtgcttctg gtcatgctca aacaccagct cttttactat
     4141 gcgaacaagg agtcacagag gatgctggga atgtgtttgc tattcaacta atgtatagtg
     4201 gcaactttga agcttttgtt caaaaaaatc aattgaatga agttcgggtg gctattggca
     4261 ttaatccaga aaacttttct tgtaagttag ctcctgagga atactttgaa acaccggtag
     4321 ctttagtgac ccattcagat cagggattaa ctggtattag tcatgaaagt cagaattttg
     4381 tactgaagca cattatgcta agtgaatttt ctaaaaaaga acgtccaatt ctaatcaata
     4441 actgggaagc tacttacttt gactttcaga tagaaaaact gttagagtta gcagatgaag
     4501 ctaagaaagt tggcattgaa ctttttgtat tagatgatgg ttggtttggc aatcgttttg
     4561 atgataatcg tgctttaggt gattgggttg ttaatgagga aaaactgggt ggaagtctag
     4621 aaagtctgat ttcagctatc catgaaagag gtttgcagtt tggactttgg ttagaacccg
     4681 aaatgatttc tgtagatagt gatttgtatc gtcgacatcc tgactgggct attcaggttc
     4741 ctgactatga gcatacttat tctcggaatc aattagtact taatcttgcc aatcctcagg
     4801 tagtagaata cttgaaaagt gtcttagatc aactcctatc ttatcatgat attgattaca
     4861 ttaaatggga tatgaaccgc aatatcacta agctagggaa tggattaact tatctagaga
     4921 cacagatgca atctcatcag tacatgctgg ggctttacga actcgtttct tatctgacag
     4981 agaagcacag ccatattctc tttgagtcct gctctggtgg tggtggacga aatgatcttg
     5041 gtatgatgcg ctatttccca caagtttggg ctagtgataa tactgatgcc attgcacgtt
     5101 taccaattca atacggttca tcctatctct atccaaccat ttctatgggg gctcatgtgt
     5161 cagcagtacc gaatcatcag atgggacgaa tgacaccatt agaaacacgt ggccttgtag
     5221 caatgatggg aaatttgggc tatgaacttg atttgacaaa tttatcagat gaagagaaag
     5281 ctacgattgc taatcaggtg aacttgtata aagaattacg accagtagtt cagttaggac
     5341 agcagtatag actaattaat cctgatactg tatccaatga agcagctgta caatttaatt
     5401 acgaaaatca aacgattgta acctacgttc gcgttttatc tgttgtagag accatggaaa
     5461 caactttaaa attaaaagat ttggatgaag agggactata taaattacag gaaaatggcg
     5521 aaatttactc aggtgcagaa ctaatgtatg cgggcctaac tgttatctta tcccaaggag
     5581 attttttgag tagacagtat atttttagaa gactatgata agggtgtata aatttataaa
     5641 ggaggctttc taatggaatg gtataaaaaa atcggacttc ttgcaactac aggtttagct
     5701 ttggttgggc tcggcgcttg ctccaactat ggtaaatctg cggatggcac agtgaccatc
     5761 gagtatttca accagaaaaa agaaatgacc aaaaccttgg aagaaatcac tcgtgatttt
     5821 gagaaggaaa accctaagat caaggtcaaa gtcgtcaatg taccaaatgc tggtgaagta
     5881 ttgaagacac gcgttctcgc aggagatgtg cctgatgtgg tcaatattta cccacagtcc
     5941 atcgaactgc aagaatgggc aaaagcaggt gtttttgaag atttgagcaa caaagactac
     6001 ctgaaacgcg tgaaaaatgg ctacgctgaa aaatatgctg taaacgaaaa agtttacaac
     6061 gttcctttta cagctaatgc ttatggaatt tactacaaca aagataaatt cgaagaactg
     6121 ggcttgaagg ttcctgaaac ctgggatgaa tttgaacagt tagtcaaaga tatcgttgct
     6181 aaaggacaaa caccatttgg aattgcaggt gcagatgctt ggacactcaa tggttacaat
     6241 caattagcct ttgcgacagc aacaggtgga ggaaaagaag caaatcaata ccttcgttat
     6301 tctcaaccaa atgccattaa attgtcggat ccgattatga aagatgatat caaggtcatg
     6361 gacatccttc gcatcaatgg ctctaagcaa aagaactggg aaggtgctgg ctataccgat
     6421 gttatcggag ccttcgcacg tggggatgtc ctcatgacac caaatgggtc ttgggcgatc
     6481 acagcgatta atgaacaaaa accgaacttt aagattggga ccttcatgat tccaggaaaa
     6541 gaaaaaggac aaagcttaac cgttggtgcg ggagacttgg catggtctat ctcagccacc
     6601 accaaacatc caaaagaagc caatgccttt gtggaatata tgacccgtcc agaagtcatg
     6661 caaaaatact acgatgtgga cggatctcca acagcgatcg aaggggtcaa acaagcagga
     6721 gaagattcac cgcttgctgg tatgaccgaa tatgccttta cggatcgtca cttggtctgg
     6781 ttgcaacaat actggaccag tgaagcagac ttccatacct tgaccatgaa ctatgtcttg
     6841 accggtgata aacaaggcat ggtcaatgat ttgaatgcct tctttaaccc gatgaaagcg
     6901 gatgtggatt aggagtagag agactatgaa aaaagtatta caaaaatatt gggcatgggc
     6961 ttttgtggtc atccccctct tgttacaagc aattttcttc tatgtgccga tgtttcaagg
     7021 agccttttac agttttacca actggacagg attgacttat aactacaaat ttgtcggctt
     7081 aaacaacttt aagctcctct tcatggatcc aaaattcatg aatgcgattg gctttaccgc
     7141 aatcattacg attgccatgg tggttggtga gattgcactc gggatcttca ttgcgcgtgt
     7201 cttgaattct aaaatcaaag gccaaacctt cttccgtgct tggttcttct tcccagctgt
     7261 tttatctggt ttgacagtgg ctttgatctt caagcaagtc ttcaactacg gtcttccagc
     7321 gattgggaat gcccttcata ttgaatttct ccaaaccagt cttttaggga ctaagtgggg
     7381 agcaatcttt gcggctgtct ttgtccttct ttggcaaggg gtggctatgc ccatcatcat
     7441 cttcctagct ggtttgcaat ctattccaac tgagattaca gaggcagcaa ggattgatgg
     7501 tgcgacgagc aagcaagttt tctggaacat tgaattgcct tacttgctac caagtgtctc
     7561 tatggtcttt atcctagccc taaaaggtgg gctgactgcc tttgaccaag tctttgccat
     7621 gaccggtggt ggtccaaaca atgccacaac ctcacttggg ctcttggttt ataactatgc
     7681 ctttaaaaac aaccaattcg gttatgccaa tgccattgcc gtaatcttgt tcttcttaat
     7741 tgtagtgatt tcgatcatcc aattgagagt atctaagaaa tttgaaattt aagaggagaa
     7801 gcatgatgaa acaagatgaa agaaaagccc tgattggcaa atacattcta ttgattctag
     7861 gatcggttct gattttagtg ccgctccttg ctaccctctt tagttccttc aaacccacta
     7921 aggatattgt agataattta tttggctttc caaccaactt cacatgggac aactttagcc
     7981 gtctcttagc tgatgggatt ggaggctatt attggaactc tgtcgtcatc actgtcttgt
     8041 ctttacttgc agtaatgatc tttatcccta tggcagccta ctccatcgct cgcaatatga
     8101 gtaaaagaaa agcctttacc atcatgtata ccctcttaat cctcggaatc ttcgtacctt
     8161 tccaagtcat catgattccg attacggtta tgatgagtaa actcggtttg gctaatacct
     8221 ttggtttgat cttgctctac ttgacctatg cgattccaca gaccctcttt ctctatgttg
     8281 gctatatcaa aatctcgatt ccagaaagtc tggatgaagc agcagagatc gatggggcta
     8341 atcaatttac aacctatttc cgcatcatct tcccaatgat gaaaccgatg catgcgacaa
     8401 ccatgatcat caatgccctt tggttctgga atgacttcat gttgccactc cttgtcttga
     8461 accgggattc caaaatgtgg actctgcctt tgttccaata caactacgca ggccaatatt
     8521 tcaacgacta cggaccaagc tttgcctctt acgtggtcgg cattatcagt atcaccattg
     8581 tctatctctt cttccaatgc catatcattt caggaatgag caacggggca gtgaagtaat
     8641 actcaatgaa aatcaaagag caaactagga agctagccgc aggctgtact tgagtacggt
     8701 aaggtgaagc tgacgtggtt tgaagagatt ttcgaatagt ataagaagag ctcatggaat
     8761 ctatagtctt tgacacagag gaagagttca gcttgttgat aattctatgt agaaaacaag
     8821 aaagtagtac tttgtttatc acagtctata ttagtacgag tttgatgata aatagagatt
     8881 taaaatgaat tttatttatg aagccagatc atatgagact tggcttcaat tagaaaaagg
     8941 agagtaatga tgccaattca aaataaaacc atgttgatta cctattctga tagccttgga
     9001 aataatctta aagacttata tgataatttg gaagagcatt ttggagatgc tattggagga
     9061 gttcaccttt taccattttt cccatcaaca ggtgatcgtg gatttgcgcc agttgactac
     9121 gacgaagtgg actcagcttt tggtgattgg gaggatgtta agcgtttagg tgagagatat
     9181 tatcttatgt ttgattttat gattaatcat atttctcgtc aatccaagta ttataaggac
     9241 tatcaagaaa aacatgaagc cagtgaattt aaagatctct ttttaaactg ggataagttt
     9301 tggccagaaa accgtccgac acagtctgat gtagatttaa tttacaagcg taaggatcgt
     9361 gcaccaaagc aagagattgt gtttgaagat ggttcagtgg aacatttgtg gaataccttt
     9421 ggtgaggagc agattgatct tgatgtgacc aaagaagtaa ctatggaatt tatccgtaag
     9481 accattcagc acttggcaag taatgggtgt gatttgattc gtctagatgc ctttgcttat
     9541 gcagtgaaga aattggatac taatgatttc tttgtggaac cagatatttg ggatttattg
     9601 gacaaagttc gagatatcgc tgctgagtat gggacagagc ttttacctga gattcatgaa
     9661 cactattcga ttcagtttaa aatagcagac catgattact atgtttatga ttttactctt
     9721 ccaatggtga cactttatac tctttacagt tccagaacag agcgtttggc taagtggttg
     9781 aagatgagcc cgatgaagca atttacgacg ctagataccc atgatgggat tggagtagta
     9841 gatgtcaagg atatcctgac cgatgaggag attgactatg cttcaaatga actctataag
     9901 gttggagcta atgtcaaacg taagtactct agtgccgagt ataacaactt agatatctac
     9961 caaatcaatt caacctacta ttcagcgctt ggagatgatg atgtcaagta ttttctcgct
    10021 cgtctaattc aagcttttgc cccaggtatt cctcaggttt actatgtggg tctattagca
    10081 ggcaagaatg acttgaaatt attagaagaa actaaagaag gtcgaaatat taatcgtcat
    10141 tactatagca acgaggaaat agcaaaagaa gtgcaacgac ctgttgtgaa ggcccttctc
    10201 aatctatttt ctttccgtaa ccgttcagaa gcctttgatc tagaagggac tactgagata
    10261 gagacaccaa cagctcacag cattgtaatc aaacgtcaaa ataaagataa gtccgtaaca
    10321 gcagtagtag aaattgattt gcaaaatcag acttatcggg taattgagaa tggagttgaa
    10381 gtatcatttt gaagccttga tatggtggat aaaatagggt ttttattttg gaaaacgttt
    10441 cctttgtttt caaattgcta aaaaagtggt acaatatagg atagcttact attatctgaa
    10501 tcagctgatt tggagagaaa ggattcattt tgaaatcaat aggctttatt gaaaagctga
    10561 aggggttgtc tagtaaagag ctgattttat tgggaattat cctaagtatc tttttaccct
    10621 tttatctttt tgtagttgta ctctgtttat atattatcag tttgattttt acaggagaca
    10681 tgaaaagtat tcttcagaaa atgggggagc atccgatgct gcttcttttt cttagctata
    10741 gtactgttat atccattctt gcacaaaatt ggatgggtct tgtggcttca gtaggaatgt
    10801 ttctatttac tattttcttt ttgcactatc agtcgatttt atcccataaa ttctttcgat
    10861 tgattttgca gttcgtcttg tttggtagtg tcttgtcagc tgcttttgcc agtttagaac
    10921 atttccaaat tgtgaagaaa tttaactatg cttttctttc acccaatatg caggtgtggc
    10981 atcagaaccg ggcagaagtg accttcttta atcctaatta ttatggaatt atttgttgtt
    11041 tctgtattat gattgctttc tatctgttta caacgaccaa gttgaattgg ttgaaagtat
    11101 tctgtgtgat tgcaggcttt gttaatctct ttggtttgaa ctttactcaa aatcgaactg
    11161 cctttcctgc tattatcgct ggagcaatta tctatctctt tacgactatt aaaaactgga
    11221 aggccttttg gcttagtatt ggggtcttcg cgattggttt gagtttcctc ttttctagtg
    11281 atttgggagt tcgaatgggt actttagact cttctatgga agaacgcatt tctatctggg
    11341 atgctgggat ggccttgttt aagcaaaatc ctttttgggg tgaagggcca ttgacctata
    11401 tgcactctta tcctcggata catgctcctt atcatgaaca tgcccacagt ctttatattg
    11461 atacgattct gagttacgga attgtgggta ccattttatt agttttgtct tctgttgctc
    11521 ctgttcgctt gatgatggat atgagtcagg agtcggggaa acgtccgatt atcggccttt
    11581 atctatcttt ccttacagtg gttgctgtgc acggaatttt tgacttggct ctcttctgga
    11641 ttcagtcagg ctttattttc ttgctagtta tgtgcagtat tccattggag catcgaatgt
    11701 tggtatcgga catgacggat taagttttat aagtttgaaa tcttctacct tgggtaggag
    11761 atttttttat actatgggca tgtcgtgcat ctgaggtgta agtcctctgt agggatccac
    11821 taccggtgaa tacaatagcg ctccccaagc ctgactgtcg tgaggtagcg gattaaaatg
    11881 gtctggggat agatcgtttt aagtctgacg caggaaataa gaattgtcag agaaagcaat
    11941 agtgaaatcg taggctatga atagaaacgt gatagcgagg agcatatagt ggataaggta
    12001 gcgagaaatt ttaaagtata aaagatagtc ttaatctata tatgtaaatc acaaaagtaa
    12061 ttgaattcgg aataaggttg gtgtgaaaag acatagtaaa ctgaatcttg aatactagaa
    12121 tgatacttct acaatattgt tataatttaa tttgaattcc aaaatcacat tgttccgtcc
    12181 gtatttcatt ttactgtatg tcttcctaaa ctccaaagtt tctgcgtcaa atttcctatt
    12241 ttcactgtag tctttgacac ttcgaacgac aatttgaatc atatattcaa aaaaaatgaa
    12301 aaatttgtgc caacaagcct aaatattctg aaaatttgtt tgatttttaa tggataatgt
    12361 gatataatgg ttgtcaatct ataattcaca taaacagagg agtagaaaat gaaaaaaaat
    12421 cgtgtatttg ctacagcagg tcttgtttta ttagcagcag gtgtacttgc agcatgcagt
    12481 tcttcaaaat catctgattc atcagcccct aaagcttatg gctatgttta tacagcagac
    12541 ccagaaacct tggactacct gatttcaagt aaaaatagta caacagtagt gacttcaaat
    12601 gggattgatg gtttattcac taacgataat tacggtaatc ttgctcctgc agttgcagag
    12661 gattgggaag tctctaagga tggtttgacc tacacttata agattcgtaa aggggttaaa
    12721 tggtttacct ctgatggaga agaatatgca gaggtgacgg ctaaagattt cgtgaacggt
    12781 ttaaaacacg cagcagataa aaaatcagaa gctatgtatt tagctgaaaa ttcggttaaa
    12841 ggcttggcag attatctatc aggaacttca acagattttt caacagttgg tgtcaaggcg
    12901 gttgatgatt atacgttaca atacactttg aaccagcctg aaccgttctg gaactctaag
    12961 ttgacctatt ctattttctg gcctctgaat gaagaattcg aaacatcaaa aggaagcgat
    13021 tttgctaaac caacagatcc gacatccttg ctttataatg gtccattctt gttgaaaggg
    13081 ttgactgcaa aatcttctgt agagtttgta aaaaatgagc aatattggga taaagaaaat
    13141 gtccacctag atactatcaa tctagcttac tatgatggat cagatcagga gtcgctagag
    13201 cgtaacttca ctagtggagc ttatagttat gcccgtcttt accctaccag ctccaactat
    13261 tctaaggttg cagaagaata caaggacaat atctattaca cacaatcagg ctctgggatt
    13321 gctggtctgg gtgtgaatat tgatcgccaa agttacaact atacttctaa aactacagat
    13381 tcagagaaag tagctactaa gaaggcattg cttaacaaag atttccgtca agccttgaat
    13441 tttgctcttg atcgctcagc ttactcagct caaatcaatg gtaaagatgg agcagcttta
    13501 gcagttcgta atttatttgt aaaaccagac tttgtttcag ctggtgagaa gacctttggt
    13561 gatttagtcg ctgctcaact tcctgcttat ggtgatgagt ggaaaggtgt gaatttagct
    13621 gatgggcagg atggtttatt caatgctgac aaggccaagg cagagtttgc gaaagctaag
    13681 aaagctttag aagcagacgg cgttcagttt cctattcatc tggacgttcc agtagaccaa
    13741 gcatcaaaaa actacatatc tcgtattcag tcctttaaac aatctgtaga aacagttctt
    13801 ggtgttgaaa atgtcgttgt tgatattcaa caaatgacaa gtgatgaatt ccttaatatt
    13861 acttactatg ctgccaatgc ttcatctgag gattgggatg tatcgggagg agtttcatgg
    13921 gggccagact atcaagaccc atctacttac ctggatattt taaaaacaac tagcagtgaa
    13981 actacaaaaa catatttagg atttgataat ccaaatagcc cttcagtagt tcaagttggt
    14041 ttgaaagaat acgataaatt agttgatgaa gctgccaaag agacaagcga cttgaatgtc
    14101 cgttatgaaa aatatgcagc ggctcaagca tggttgacag atagttcact ctttattcct
    14161 gctatggctt cttctggtgc agcaccagtg ctttcacgaa ttgttccatt tactggagct
    14221 tctgcgcaaa caggctctaa ggggtcagat gtttacttca aatatttgaa attacaagat
    14281 aaagtggtga ctaaggaaga gtatgaaaaa gctcgtgaaa aatggttgaa agaaaaagct
    14341 gaatcaaatg agaaagctca aaaagaattg gcaagtcatg tgaagtaaat aatttctaat
    14401 agaactttct ctccatgagg agaaggttct tttgggattt ttaaaggaaa taatatgaaa
    14461 aaatatattt ttatgcgtgt tttgcggtca ttggtttcga ttttcttagt aacgactttg
    14521 acctacacga ttatctatac cttggttcct cgaaaattga ttttcaagca ggatcctaac
    14581 tataataaaa ttgcgacaac ggctgataaa cgtgataact atgaaaatac tgtgtttgag
    14641 cgtatgggct acattgagta ttacgatact aaagagttgc aagaaaaggc aagtagcatg
    14701 gattcttctg taacagtaga agcaaatgcg accaataaag ctatttatga aaagtacatc
    14761 aatcaattag gtcatggttg gactttggga gaatttactg aaagtggtca attctatgct
    14821 actcgtgaaa ttccaatttt tgaacgtgtt tttcacttct atgctaactt gattgacatt
    14881 gaccatacaa ataaaatcca agaccctgaa aatccagact tgaaacgcta ccttcgtttt
    14941 gaaaatgatc cagctatcgg atggtcattg gtcggttcag gaactaaaca taaatatctc
    15001 ttgtacttta acagtcagtt cccatttgtt catcaaaact ttgtgaactt gaatttaggt
    15061 gactcttacc caacctatgc taatacacca gttcttcagg ttattactca aggtcaagga
    15121 caaaccaaaa ctgcccaagt tcagttccca acaggtaaga aaacgtcttc tgtaaatatt
    15181 tactcaagaa cctacaagtc acctagtcag gctgactctc gtgaagtagc tagctatggg
    15241 aaagatgatc cttatacagc gactgaaagt aattaccaat atccatctat gattgtcagc
    15301 tctgctatta ctggtttgat tggtttggtt cttgcctatg ctcttgccgt gccacttggt
    15361 tcagccatgg ctcgtttcaa gaacacttgg attgatagcc tctcaacagg gactttgacc
    15421 ttcttgcttg ctcttccaac gattgccttg gtttacatcg ttcgattgat tggatcatct
    15481 attgcccttc cagattcatt ccctatcttg ggagctggag attggcgttc ttacgtttta
    15541 ccagcagtca tccttggttt gttgggtgct cctgttacag ccatttggat tcgtcgttac
    15601 atgattgact tgcaatctca agactttgtt cgtttcgctc gtgcaaaagg tttgtctgaa
    15661 aaagaaattt caaacaaaca catctttaaa aatgccatgg ttccgctggt ttcaggaatt
    15721 cctggtgcca ttattggggt tatcggtggt gcaaccctta ctgaaacagt cttcgccttc
    15781 ccaggtatgg gtaaaatgtt gattgactct gtaaaagcat ctaataactc tatggtcgtt
    15841 ggtcttgtct tcatctttac atgtatttct atcttctcac gtcttttggg agatatttgg
    15901 atgactatta ttgacccacg tattaaattg actgagaaag gaggcaaata atgtctacaa
    15961 tcgataaaga aaaatttcag tttgtaaaac gtgacgattt tgcctctgaa actattgatg
    16021 cgccagcata ttcttactgg aaatcagtgt ttaaacaatt tatgaagaaa aaatcaactg
    16081 tagtcatgtt gggaatcttg gtagccatca ttttgataag tttcatctac ccaatgtttt
    16141 ctaagtttga tttcaatgat gtcagtaagg taaacgactt tagtgttcgt tatatcaagc
    16201 caaatgcgga gcattggttc ggtactgaca gtaacggtaa atcgctcttt gacggtgtct
    16261 ggttcggagc tcgtaactcc atcctcattt ctgtgattgc gacagtgatt aacttggtta
    16321 tcggtgtttt tgtcggtggt atttggggta tttcaaaatc agttgaccgt gtcatgatgg
    16381 aagtttacaa cgtcatctca aacatcccac ctcttttgat tgttattgtc ttgacttact
    16441 caatcggagc tggattctgg aatctgattt ttgccatgag cgtaacaaca tggattggta
    16501 ttgccttcat gatccgtgtg caaatcttgc gctatcgtga cttggaatac aacttggcgt
    16561 cacgtacttt gggaacacca accttgaaga ttgttgccaa aaatatcatg cctcaattgg
    16621 tatctgttat tgtgacaacc atgactcaaa tgcttccaag ctttatctca tacgaagcct
    16681 tcttgtcttt cttcggtctt ggattaccga ttacagtgcc aagtttgggt cgtttgattt
    16741 cggattattc acaaaacgta acaaccaatg cttacttgtt ctggattcca ttgacaaccc
    16801 ttgtcttggt atccttgtcc cttttcgtag ttggtcaaaa cttagcggat gctagtgatc
    16861 cacgtacaca tagataggag tagaaatgac aaaagaaaaa aatgtaattt tgactgctcg
    16921 cgatattgtc gtggaatttg acgttcgtga caaagtattg acagccattc gcggcgtttc
    16981 ccttgaacta gtcgaaggag aagtattagc cttggtaggt gagtcaggat caggtaaatc
    17041 tgttttgaca aagaccttca caggtatgct cgaagaaaat ggtcgtattg cccaaggtag
    17101 tattgactac cgtggtcagg acttgacagc tttatcttct cacaaggatt gggaacaaat
    17161 tcgtggtgct aagattgcga ctatcttcca ggacccaatg actagtttgg accccattaa
    17221 aacaattggt agtcagatta cagaagttat tgtaaaacac caaggaaaaa cagctaaaga
    17281 agcgaaagaa ttggccattg actacatgaa taaggttggc attccagacg cagatagacg
    17341 ttttaatgaa tacccattcc aatattctgg aggaatgcgt caacgtatcg ttattgcgat
    17401 tgcccttgcc tgccgacctg atgtcttgat ctgtgatgag ccaacaactg ccttggatgt
    17461 aactattcaa gctcagatta ttgatttgct aaaatcttta caaaacgagt atcatttcac
    17521 aacaatcttt attacccacg accttggtgt ggtggcaagt attgcggata aggtagcggt
    17581 tatgtatgca ggagaaatcg ttgagtatgg aacggttgag gaagtcttct atgaccctcg
    17641 ccatccatat acatggagtc tcttgtctag cttgcctcag cttgctgatg ataaagggga
    17701 tctttactca atcccaggaa cacctccgtc actttatact gacctgaaag gggatgcttt
    17761 tgccttgcgt tctgactacg caatgcagat tgacttcgaa caaaaagctc ctcaattctc
    17821 agtatcagag acacattggg ctaaaacttg gcttcttcat gaggatgctc caaaagtaga
    17881 aaaaccagct gtgattgcaa atctccatga taagatccgt gaaaaaatgg gatttgccca
    17941 tctggctgac taggaggaag gaaatgtctg aaaaattagt agaaatcaaa gatttagaaa
    18001 tttccttcgg tgaaggaagt aagaagtttg tcgcggttaa aaatgctaac ttctttatca
    18061 acaagggaga aactttctcg cttgtaggtg agtccggtag tgggaaaaca actattggtc
    18121 gtgctatcat cggtctaaat gatacaagta atggagatat catttttgat ggtcaaaaga
    18181 ttaatggtaa gaaatcgcgt gaacaagctg cggaattgat tcgtcgaatc cagatgattt
    18241 tccaagaccc tgccgcaagt ttgaatgaac gtgcgactgt tgattatatt atttctgaag
    18301 gtctttacaa tcaccgttta tttaaggatg aagaagaacg taaagagaaa gttcaaaata
    18361 ttatccgtga agtaggtctt cttgctgagc acttgactcg ttaccctcat gaattctcag
    18421 gcggtcaacg tcaacgtatc ggtattgccc gtgccttggt catgcaacca gactttgtta
    18481 ttgcagatga gccaatttca gccttggacg tttctgtacg tgcccaagtc ttgaacttgc
    18541 tcaaaaaatt ccaaaaagag ctcggcctga cctatctctt catcgcccat gacttgtcgg
    18601 ttgttcgctt tatttcagat cgtatcgcag ttatttacaa gggtgttatt gtagaggttg
    18661 cagaaacaga agaattgttt aacaatccaa ttcacccata tactcaagcc ttgctttcag
    18721 cggtaccaat cccagatcca atcttggaac gtaagaaggt cttgaaggtt tacgatccaa
    18781 gtcaacacga ctatgagact gataagccat ctatggtaga aatccgtcca ggtcactatg
    18841 tttgggcgaa ccaagccgaa ttggcacgtt atcaaaaagg actaaactag taatggtttt
    18901 ataatttcca tgtcaatttt tatggaaatt ataaaacttt ttttgttgga ctgatttgaa
    18961 aaactttgaa agcttcctga aagaattttc gaaaaatttt aaaaaattct gaaatattat
    19021 tgacaggtga tgaaaaaggt ggtaggatag gaagcatcaa aaaagaaagc agaaaaagaa
    19081 atgaataaga gacaagccgt aactatgaat caaaactttt actttagcta ctttagatag
    19141 tgtttgtaga catgtcacca tggatgcaaa cagttcaagc aatttgtttg tatctatgtg
    19201 gctaagattt ttgattgtgg tccatataga tgaatatcta aatggactag ctaagttgta
    19261 acttgttagg ttatttcaag tatccgttta gatagtatct aggcggtttt ttgttttatc
    19321 ttttctgaga aggagtttca ttatgaaagt tgttcgaaaa tcactagccc cctcttggta
    19381 gtggggctcc tcttgacttc tctgatcagt ttgcatcagt tgaaggcaga taagaaaaag
    19441 atgtctttcg tatcggtatt tcgcaattta ttacccacca gtccttatac gctactagag
    19501 aaggatttgt ggatgagctg gccaaacaag gctatgttga gggggaaaat attgagattg
    19561 atttgcaaaa tgcacaggga gaacaaagaa atctaaaaac gatttcccag caactagcag
    19621 aatccagcga tgtcgttcta gccatcgcaa ggccttctgc tcagagcttg gccaatacga
    19681 cgcaaacgac accagttatc ttttcagctg taacagaccc agtcagtgcc aagttggttg
    19741 agtcaagaga acatcctggg ggcaatgtaa ctgggacaag cgatcagtca tcagatgcca
    19801 tttcaaccca aatcaatttg ataaagaaag tgttgctaaa ggctaaaacg attggaatcc
    19861 tctatactca gagcgagccc aattcggttg tttaaaagga tgaagctaag cgacttctga
    19921 aagaaaaagg ctttaccgtt gttgaaaaaa caatcttgga cagtaataac gtcaaggcgg
    19981 cagcagagag tttgatggca gaggtggata tggtttttgt cccaactgat aatatcattt
    20041 cgtcaaccat ggaaacagtt aagcaggttt ctattaaaca caaggttcca gtattcggtg
    20101 gttcaacaga aatgattgct gttggtggct tgtataacta cggaaccaat tatgaagaat
    20161 tgggaagaca gacagctcgc atgctgattc gtgttttaaa aggtgaggaa ccagaaaata
    20221 tagcagttga gttgcctgaa aaactggaat tgcataccaa tcaagaaatg gcagatgcat
    20281 tgggaattga tattagtaag ttagaaggca aggaataagg aggattaaga tgaattttgt
    20341 gttatctagt ttatcagaag gtttgttgtg gtcgattatg gcaattgggg tctacttgac
    20401 tttccgtatt ttggatattg cggatatgac tgctgaggga gcctttcctc tgggggcagc
    20461 agtcgttgta tcacagatac aggcaggggc aaatccttgg cttgcgactt tacttgcttt
    20521 ggtggcaggt atggtagcag gtcttgtatc aggaatgctc cacaccaata tgaaaatccc
    20581 agctctcttg acagggattg tgaccttgac agggctctat tcaatcaata ttaaaattat
    20641 gggcagtgta cccaatcttt ccttgggaga ttctgcaacc gtctttaaac agttggcgag
    20701 cttgggtctg acaaatgaag gagctgtttt ctcacttagt ttagtctgtc tcttacttgt
    20761 ttggtcttga cccttttgat gaaaacagag attggtttgg tcttgcgttc gactggggac
    20821 aatatcccga tgagtgaggc caatggagtc aatgtaaaca ccatgaagat tgttggttac
    20881 atgatttcaa acggtttgat tgccctatgt ggttccttgt ttgcccaaaa tgatggattt
    20941 tcagatgtga cttctgggac aggaaccatt gttgttggtt tgagctcgga agtcttgata
    21001 cacgacttga ccattggagg ccgcttatta tccatcggaa tcggtgctat tgtttaccgt
    21061 ttgattattt taaatatcta tgaaattcca aatctagatc aaaacttggt acgtctcttt
    21121 aatgcaatct tgctcgcttt ggttttattt gcaccagaat tgcaaaagag attaaagatt
    21181 cgtggtctga aattgagaaa tgaataggag gagaaagtta tggcaagttt gttaacactt
    21241 gaaaatattc acaagacatt tgaagcaggg acggtcaatg agaaccatgt cctcaaagga
    21301 ttagatttag aggttgaaga gggagatttt atctctgtga tttgtggaaa tggagcaggg
    21361 aaatccactt tgatgaatat cttggctggc aatctatcgg tggacgaggg ggacctttta
    21421 ttggcaggga aatccattaa acatctgaat gtaagaaaga gagccaatga tatcgcccgt
    21481 gtttttcaag atcctaagat gggaacagct tctcgtttga cgattgagga gaatatggct
    21541 attgccttga gatgtgagca aaagagaggg cttggttggg gtgtgaagga gaaggataga
    21601 atccagttcc aagaggcctt gaaggagttg aatattggtc ttgaaaaccg cttaaaagta
    21661 gatactcaat atctctcagg aggacaaaga caggctttga ccctagtcat ggcagctctg
    21721 gtgaaaccca aacttttact tttggatgaa catactgcgg cacttgacct gaaaaccagt
    21781 caaatggtga tgaacttgac gcaaaagatt gtggagcacc atcagtggac gactttgatg
    21841 attacccatg atatgaatca tgcgattgag tacggcaatc gtctcattat gctctatcaa
    21901 ggaaagatag tggtagatgt caagggagaa gagaaaaagc atctgacagt tgaagacctt
    21961 atgcatctct tccagaaaaa tagtggccaa agtctggtca gtgatgaatt agttttggga
    22021 taaagaaaaa ggctgagatc tggtgtctca gtcttttatt tcttgcgttt tgcaaaatct
    22081 acaaatctaa atttttctaa cttatggcga ctttcggtaa actgaaattg gcgtccgtct
    22141 tggagataga cttttgattt aatcgaaacg acataagggt ctttgccaat gtccatgaga
    22201 atcttgtctc ggtcgcttga gtggtcaatg gtgatttcct tctgagcata atcaataagg
    22261 agtttgaggc cattttctat ataagaatag atagactgct cagcaatttg gcgagtgaga
    22321 tttggaatta gttccatatc cagatagtct gtatccagaa cggataccag atcatccacc
    22381 acacgctggc ggaccacctt ccaaaccatc cgaaactctg ggaaaccggt tatcagtgag
    22441 gattttttat caataataat cttgtccaga ctgaccacgt tggttttaga gcgcagtcca
    22501 agttctttaa ctagttcttg gtagctggtt aggttggata cagggaaatt gacggtttct
    22561 tctttgacga cttgagaacc ttgccctctt atctttttga tcaatccttc ctcttggagg
    22621 agagacaggg cctttcggac ggtatcacga ctcacttgat attgctccat aaggtcgtgc
    22681 tcgctaggaa ggaaatctcc gacagcgtaa gtctcgtttt gaatggtttc ttggatttgc
    22741 ttaaataatt gttggtattt cttcattttg cctgcttaaa taggttttta gtctagttta
    22801 atgaacacct gtatctattt taccatacaa gtgctagaaa atttacaaaa aaacaccctt
    22861 tttataccaa acaagttgta gacaagtttg gtatcgttta caatatactt atcaaatcaa
    22921 acttgctttg acaagtataa ggagaatcaa atgggaaaat ttgaacaaga agccaaagat
    22981 ctgcttcagg caattggagg caaagaaaat gtgactgccg taactcactg tgcgacacgg
    23041 atgcgctttg ttttaagaga tgataagaag gctaatgtta aagctatcga gtcaattcca
    23101 gctgttaaag gaacttttac aaatgcaggt caatttcagg taatcattgg aaatgacgtg
    23161 cccatctttt ataatgattt tacagccgtt tcaggtattg agggtgtttc caaagaagca
    23221 accaagtctg cagctaagag taatcaaaac gtggttcaag gtgttatgac cactctggcg
    23281 gaaatcttta ctccgattat tccagccttg attgttggtg gattgatcct cggtttccgt
    23341 aatgtcttgg aaggtgtcca ttggtcgatg ttggatggca agaccatcac agaatcctct
    23401 cagttttggg caggggttaa tcacttcctc tggttgcctg gtgaagctat cttccagttc
    23461 ttaccagtag ggattacttg gtctgtttct cgtaagatgg gaaccagcca aattttgggg
    23521 attgttctcg gaatctgttt ggtatcgcct cagttgctca atgcctatgc ggttgcttca
    23581 acgccagcag ctgatatcgc ggcaaactgg gtttggaatt ttggttattt tactgttaat
    23641 cgtattggtt accaagccca agttatccca gccttgcttg caggtttgag tctgtcttat
    23701 cttgaaattt tctggcgcaa acatatccca gaagtcattt ccatgatttt tgtacctttt
    23761 ttgtcattga ttccagcctt gattttggct catactgttt tgggaccaat cggttggaca
    23821 attggacaag gactttcatc agttgtcttg gcaggtttaa ctggtccagt taaatggctc
    23881 ttcggtgcaa tttttggtgc cctctacgct ccatttgtca tcacaggtct gcaccatatg
    23941 accaatgcca ttgatacaca attgattgcg gatgctggtg gcactgccct atggccaatg
    24001 attgctcttt ctaatattgc tcaaggctca gccgtgtttg cctattattt catgcatcgc
    24061 catgatgagc gtgaggctca ggtttcactt cctgcaacca tttcagccta tctcggtgtt
    24121 acagaaccag ctctttttgg ggttaacgta aaatatattt atccatttgt tgctgggatg
    24181 actggctcag cccttgcagg catgttatcc gttactttta atgtaactgc ggcttctatt
    24241 ggtatcggtg gtttgccagg tattctctct attcaacctc aatacatgct gccatttgca
    24301 ggaactatgc tagttgcgat tgttgttcca atgctcttga ctttcttctt tcgcaaggct
    24361 ggtctcttca caaaaataga gggcgatacg aacttgcagg cagaattcgt tgctcaagaa
    24421 gaagcagaat ttgtgagcca tgaaccagta gaacttactt cggtagaaat tatcagccca
    24481 ctaactggcc aagtgaaaga attgagtcaa gcgacggatc ctgtttttgc atcaggtgtc
    24541 atggggcaag gtctggtcat tgaaccaagc caaggtgagt tgacctctcc agttaatgga
    24601 acagtgacgg ttcttttccc taccaagcat gccatcggca ttgtctctga cgagggagtt
    24661 gaattgctca tccacatcgg tatggataca gtaggtcttg atggcaaagg ttttgaaagt
    24721 cttgtagtcc aaggagatca cgttatagtt ggccagcaac tgattcgttt tgatatggat
    24781 gtcattaagg ctgcaggtct ggtgacagaa actcctgtta tcatcaccaa ccaagatgct
    24841 tatacagcga ctattactgg aacttatccg acaacgatcc aagctggagc atctctcatg
    24901 gtcgctacac gaatctaata ctcttcgaaa atctcttcaa accacgtcag ctctatctgc
    24961 aacctcaaaa tagtgttttg agcaacctgt ggctagcttc ctagtttgtt ctttgatttt
    25021 cattgagtat aattgaaata gcacaaatag ggacggaaga ctgcttcctc ccttttatca
    25081 gaaaaggaga aacagatgac acttgataaa ggaaaagtag tctatcaaat ctatccaaaa
    25141 tcttacaaag acaccactga aaacggtatt ggggatttcc gtgggattat cgaaaaaatt
    25201 ccctatttag ccaaactagg tgtggatatg gtctggctca atccattcta tccaagccct
    25261 caacgggata atggttacga tatttcagat tatatggcag tggatcctct ttttggtgat
    25321 atggctgatt ttgaggaaat ggtgtgtgtc ggtaaagagc acaagattga ctttatgctg
    25381 gacatggtac ttaatcattg ttcgacagag catgaatggt ttcagaaagc cctagctggt
    25441 gataagtatt atcaagactt tttcttcatc caagaccaac caacagattg gcagtctaag
    25501 tttggtggtt ctgcatgggc gcctttcggg gatactggga aatactatct tcacctattt
    25561 gatgagaccc aggctgacct taactggcgc aatcccaatg tccgtaagga gcttttcaag
    25621 gttgttaatt tctggcgcga caagggtgtc aaaggttttc gatttgatgt gatcaatttg
    25681 atcggtaagg acgaggtttc agtggactgt cctgaaaatg aagggaagcc agcttataca
    25741 gacaaaccca tcgttcataa ctatttgcgt atgatgaacc aagccacttt tggatctgac
    25801 gatagcttta tgacagttgg ggaaatgtct tctaccacca tggaaaactg tgtcctctat
    25861 tcgtcgcctg atcgtcagga attatccatg acctttaatt ttcatcacct caaggtggac
    25921 tataaggatg gacaaaagtg gaccttggct ccctttgatt ttgaagagtt gaagagtctt
    25981 taccatagtt ggggtaagga aatgagtgat aaagacggtt ggagtgccct cttttggaac
    26041 aatcacgatc aaccacgtgc cttgaaccgt tttgtcgata ttcaaaactt ccgcaaggaa
    26101 ggagccacca tgctagcagc cagcattcac ctgtcacgtg ggacacctta tatctacatg
    26161 ggtgaggaaa tcgggatgat tgacccagac tatgattcta tggctgatta tgtggatgtc
    26221 gaatcgctta atgcctatca gatgctctta gaagaaggaa agagtcagca agaagccttc
    26281 cagattattc aagctaagtc gcgtgataat tcacgcattc ccatgcagtg ggatgcttcg
    26341 gaaaatgcag gattttcaac aggcactcca tggttgaaag ctggtaaatc ctataaagat
    26401 atcaatgtag aaaatgaaat ccagggtccg atttttacct tctatcaaga cttgattcga
    26461 ctgcgtaagg aaatgccaat catttcagaa ggaagttaca aaccagcctt tgaagatagc
    26521 aaacaagtct acgcttttga acgtcagttt gaggatcaaa agttactagt gctcaataac
    26581 ttttatgcta aagaagtgga aatcgacttg ccagcagtct accaaaatgg acagattttg
    26641 atatcaaact atgaagatgc agaagtgtct gagaaaattc tactcaaacc ctatcaaaca
    26701 ctggctattt atgtaaatta atactcttcg aaaatctctt caaaccgtgt cagttttatc
    26761 tgcaacctca aagcggtgct ttgagcaacc tgtggctagc ttcctagttt gctctttgat
    26821 tttcattgag tataaactta gcaaggagac ctctttttca gaaaagcagg gttctttttg
    26881 ctgtctattc catccctgaa agtgtagaac aaactggtca gggtggctag tttgtggtat
    26941 aatgaaaggg attcaaaaag aaacaggaga aaaatgatgg atttactttt agcaattgta
    27001 ttgattgtgc tagcttttct aggaggagct cttggaggaa tgtacttggt tcgtaagcaa
    27061 atcgaaaaag aatttgctga caacccacgt ttgaatgctg aagcagttcg tactcttttg
    27121 agtgcaaatg gtcaaaaacc aagcgaagct aaggtacaac aagtttacca ccaaatcatc
    27181 cgccaacaaa aggcagccct tgctaacaat aaaaagaaaa aataaataag gaaaagtctg
    27241 ggatgaaagt tccagacttc tcactatgtt ggttatggtt tagggatgag actttttcct
    27301 tgcattctat tgatatttga ttatgattaa gagagaggta gttgacattc tatcaagctg
    27361 tcggtcagtt cgttcagtac aaggaatcat aatgaacgat caatcagaaa aaagactaga
    27421 aagaagactg tatggataat cgaccaattg gttttttgga ttcgggtgtc gggggcttga
    27481 ccgttgtgcg cgagctcatg cgccagcttc cccatgaaga aatcgtctat attggagatt
    27541 cggcgcgggc gccctatggc ccccgtcctg ctgagcaaat tcgtgaatat acttggcagc
    27601 tggtcaactt tctcttgacc aaggatgtca aaatgattgt cattgcttgt aacactgcga
    27661 ctgcggtcgt ctgggaagaa atcaaggctc aactagatat tcctgtcttg ggtgtaattt
    27721 tgccaggagc ttcggcagcc atcaagtcca gtcaaggtgg gaaaatcgga gtgattggaa
    27781 cgcccatgac ggtacaatca gacatatacc gtcagaaaat ccatgatctg gatcccgact
    27841 tacaggtgga gagcttggcc tgtcccaagt ttgctccctt ggttgagtca ggtgccctgt
    27901 caaccagtgt taccaagaag gtggtctatg aaaccctgcg tcccttggtt ggaaaggtgg
    27961 atagcctgat tttgggctgt actcattatc cactccttcg ccctattatc caaaatgtga
    28021 tggggccaaa ggttcagctc atcgatagtg gggcagagtg cgtacgggat atctcagtct
    28081 tactcaatta ttttgaaatc aatcgtggtc gcgatgctgg accactccat caccgttttt
    28141 acacaacagc cagtagccaa agttttgcac aaattggtga agaatggctg gaaaaagaga
    28201 ttcatgtgga gcatgtagaa ttatgacaaa taaaatttat gaatataagg atgaccagga
    28261 ctggtatgtt gggtcttata gtatttttgg tggcgttaac agtttgagcg actataagac
    28321 agattttcct ctgtttgaat tctccaaaat atttggagat gaagagtatg gtttcccgct
    28381 ttcagttact gttttacgct atggttctac ctaccgtttg ttctcctttg tggtagacat
    28441 gcttaatcaa gaaatgggac gaaacttgga agttattcaa cgtcatgggg ccctgctctt
    28501 ggttgaaaat gggcaactct tgtatgtaga attgcctaaa gaaggggtca atgttcatga
    28561 tttctttgag acaagcaagg tcagagaaac cttgttgatt gcgactcgta acgaaggtaa
    28621 aaccaaggaa ttccgagcta tctttgataa gttaggctac gatgtggaaa atcttaatga
    28681 ctaccctgac ctgcctgaag tagcagaaac aggtatgacc tttgaagaaa atgcccgcct
    28741 taaggcagaa accatttctc aattaacggg caagatggtt ttggcagatg attctggtct
    28801 caaagtcgat gtccttggtg gcttaccagg cgtctggtca gctcgtttcg caggtgtggg
    28861 agcaactgac cgtgaaaata atgccaaact cttgcacgaa ttggccatgg tctttgaact
    28921 caaggaccgc tcggctcagt tccacacaac cctagtcgta gccagcccaa ataaggaaag
    28981 tttagttgtt gaagcagact ggtcaggtta tattaacttt gaacctaagg gtgaaaatgg
    29041 ctttggctat gatcccctct tccttgtagg agagacaggt gagtcatcag ctgaattaac
    29101 cctggaagaa aaaaatagtc aatctcaccg tgccttagcc gttaagaaac ttttggaggt
    29161 atttccatca tggcaaagca aactatcatt gtaatgagcg attcccatgg cgatagcttg
    29221 attgtggaag aagtccgtga tcgctatgtg ggcaaagtcg atgccgtttt tcataacggc
    29281 gattctgaac tacgtccgta ttctccactt tgggagggca tccgcgttgt taaagggaac
    29341 atggacttct acgccggcta cccagaacgt ctggtgactg agcttggttc gaccaagatt
    29401 atccaaactc atggtcactt gtttgacatc aatttcaact ttcaaaagtt ggactactgg
    29461 gctcaggagg aagaggccgc tatctgcctc tatggtcact tgcatgtgcc aagtgcttgg
    29521 atggaaggca agatcctctt tctaaatcca ggttctatca gtcaaccacg aggtaccatc
    29581 agagaatgtc tctatgctcg tgtggagatt gatgatagtt acttcaaagt ggactttttg
    29641 acacgagatc acgaggtgta tccaggtttg tccaaggagt ttagccgatg attgccaagg
    29701 agtttgagac tttcttgttg gggcaggagg aaactttttt gacccctgct aaaaatctag
    29761 ctgtgttgat tgatacccac aatgcggatc atgcgaccct cttgctcagt cagatgacct
    29821 atacccgtgt tcccgttgtg acagatgaaa aacagtttgt tgggacgatt ggactcagag
    29881 atattatggc ttatcagatg gagcatgact tgagccaaga aatcatggcg gatacggata
    29941 tcgttcatat gacaaaaacg gacgtagcgg ttgtttcgcc tgatttcacc attacggagg
    30001 tcttgcacaa gctagtagat gagtccttct taccggttgt ggatgcagag ggtattttcc
    30061 aagggattat tacgcgcaag tccatcctca aggccgttaa tgccctcttg catgacttta
    30121 gtaaggaata tgagattcga tgccaatgag agacaggatt tcagcctttt tagaggaaaa
    30181 gcagggcttg tctgtcaatt ccaagcagtc ctataagtat gatttggagc aatttttaga
    30241 catggtaggt gagcggattt ctgagaccag tctcaagatt taccaagccc agctagccaa
    30301 tctaaaaatc agcgcccaga agcgaaagat ttcggcctgt aaccaatttc tatactttct
    30361 ctatcaaaaa ggagaggtgg acagctttta ccgcttggaa ttagccaaac aagctgaaaa
    30421 gaagacggaa aagccagaga ttctatacct agactctttt tggcaggaaa gcgaccatcc
    30481 agagggccgc ttgctagcgc tcttaatcct agaaatgggg ctcttgccca gtgagatttt
    30541 agccatcaag gttgcggaca tcaatctgga ttttcaggtg ttgcgaatca gcaaggcttc
    30601 ccaacagagg attgtcacca ttcccacggc cttgctttca gaattggaac ccttgatggg
    30661 gcagacctat ctttttgaaa gaggagagaa accctattct cgtcagtggg cctttcgtca
    30721 gttagaatct tttgtcaagg agaaaggttt tccatcctta tcagctcaag tcttacgtga
    30781 acagtttatt ctaagacaaa tagaaaacaa ggtcgatttg tacgaaattg caaaaaaatt
    30841 aggattaaaa acagtcctga ccttagaaaa atatagataa tggatattaa attaaaagat
    30901 tttgaaggac ccctggactt gctcttgcat ctggtttcta agtaccagat ggatatctac
    30961 gatgtgccca ttacggaagt catcgaacag tatctagcct atgtctcaac cctgcaggcc
    31021 atgcgtctgg aagtgacggg tgagtacatg gtcatggcta gtcagctcat gctgattaag
    31081 agtcgtaaac tccttccgaa ggtagcagaa gtgacagact tgggggatga cctggagcag
    31141 gacctcctct ctcaaatcga agaatatcgc aagttcaagc tcttgggtga gcacttggaa
    31201 gccaagcacc aagaacgggc ccagtattat tccaaagcgc cgacagagtt gatttacgaa
    31261 gatgcggagc ttgtgcatga caagacgacc attgacctct ttttggcttt ttcaaatatc
    31321 ctagccaaga aaaaagagga gtttgcacaa aatcacacga cgatcttgcg ggatgagtat
    31381 aagattgagg acatgatgat tatcgtgaaa gagtccttgg ttggacgagc tcaattgcgc
    31441 ttgcaggatt tgttcaagga agcccagaat gtccaagagg tcatcaccct ctttttggca
    31501 accctagagt taatcaaaac ccaggagttg atcctcgtgc aagaggagag ttttggagat
    31561 atctatctca tggaaaagaa ggaagaaagt caagtgcctc aaagctagac ttgatagaga
    31621 ggaaagatga gtactttagc aaaaatagaa gcgctcttgt ttgtagcggg tgaagatggg
    31681 attcgggtcc gccagttagc tgaactcctc tctctgccac cgacaggcat ccagcaaagt
    31741 ttaggaaaat tagcccagaa gtatgaaaag gacccagatt ccagtttggc tttgattgag
    31801 acaagtggtg cttatagatt ggtgaccaag cctcaatttg cagagatttt gaaggaatac
    31861 tctaaggcgc ctatcaacca gagcttgtct cgggctgccc ttgagacctt gtccattatt
    31921 gcctacaaac agccgattac gcggatagaa attgatgcca tccgtggagt taactcgagt
    31981 ggagccttgg caaagttgca ggcttttgac ctgataaagg aagacgggaa aaaggaagta
    32041 ttggggcgcc ccaacctcta tgtgactacg gattatttcc tagattacat ggggataaac
    32101 catttagaag aattaccagt gattgatgag cttgagattc aagcccaaga aagccaatta
    32161 tttggtgaaa ggatagaaga agatgagaat caataagtat attgcccacg caggtgtggc
    32221 cagtaggaga aaagcagaag agctgattaa gcaaggcttg gtgacggtta acggccaagt
    32281 ggtgcgtgaa ctagcaacca ctatcaagtc aggcgacaag gtcgaagttg aaggtcaacc
    32341 tatctacaac gaagaaaagg tctactatct gcttaacaaa ccacgcggtg tgatttccag
    32401 tgtgacagat gataagggtc gcaagacggt tgtcgacctc ttgcccaatg tcaaagagcg
    32461 tatttaccct gtgggtcgtt tggactggga tacatcaggt gtcttgattt tgaccaatga
    32521 tggggacttt acagacgaga tgattcaccc tcgtaatgag attgacaagg tttatgtcgc
    32581 gcgtgttaaa ggtgtggcca ataaggacaa tctccgcccc ttgacccgtg gtcttgagat
    32641 tgatggtaag aaaaccaagc cagctgttta tgaaattctc aaagtggacc cagtcaaaaa
    32701 tcgctctgtg gtgcagttga ccatccatga agggcgtaac catcaggtta aaaagatgtt
    32761 tgaagctgtt ggtctccaag tagataagtt gtctcggact cgtttcggac acctagactt
    32821 gacaggactc cgtccaggag aatcccgtcg tcttaataaa aaagaaatca gccaactaca
    32881 caccatggct gtaactaaga aataatgaaa cgaattttaa tagcgcctgt gcgcttttac
    32941 caacgtttta tctcaccagt ctttccaccc tcttgtcgct ttgagctgac ttgttccaac
    33001 tacatgattc aggctattga aaaacatggt tttaaggggg tattgatggg cttggctcgg
    33061 attttacgtt gtcatccctg gtcgaaaaca ggtaaggatc ccgttccaga ccacttttcc
    33121 cttaaacgaa atcaagaagg ggaatgaggt ggggtaaata gatttcaaaa tgataaaaac
    33181 gcatcctatc aggtttgagt aaacttgata ggatgcgttt tagaatgtca aaattttata
    33241 ctcttcgaaa atctcttcaa accgcgtcag ctttcatctg caacctcaaa acagtgtttt
    33301 gagcaacctg cggctagttt cctagtttgc tctttgattt tcattgagta ttaaattgag
    33361 tttgaagtgg cttatttcaa agctttttgt atgtcttcaa tcatgagttt tgttgattca
    33421 agtccgcctc cgcttagata ccagaggtct ggtgttagtt ggataatctt accattttta
    33481 gcagcaggtg tttcagcgat aagggcattt tctaggacac cgtcgttgct agagttgtcc
    33541 ccaccgatgg caagggtacg gttgatgaca aagaggatgt cagggttgat ttctttgaca
    33601 ctttcaaagc tgacttcttg tccgtggcgt gagtcttcaa attttgtatc agttggtttg
    33661 aatttcaagg tttggtacaa gaaagagaaa cgagatttgg caccaaagtc tgccattttt
    33721 ccttcattaa ggaggatcgc aagggctttt ttgtcagagc tttcattttt agtagcgact
    33781 tcttggatgc tcttgtctag cttggccaat tcttccttgg ctttctgtgt accagtttcg
    33841 ccgaaggcac ttgctaagga ttcgatatta gccttggtag aagtccagta gtcatccttg
    33901 cttgcttgga agagaacggt tggggcgatt tctttgaatt tgtctacgaa tttttgtgta
    33961 cgtggtgaag cgataatcaa atcaggctca agggcggcga tagcttctaa atcaggttct
    34021 ttcatagaac caacattttt gacagttccc actaggtctt ttagataagt cggaacagtt
    34081 tttgtaggca ttccgacgat atttttttca aatcctaaag cgcgaatagt atccgcagcg
    34141 ccgaggtcaa aggtcacaat cttttcagga actttggaaa gtttgacctc gtccagtgaa
    34201 cttttaatgg ttatctctgt tggagcagag ctactggtct ctgtctgact agtgcttgag
    34261 tttgtactac atgcaccaag taggagcaag aagctggcca ctagggcagt gaaataaagt
    34321 ttaagggatg ttttcataat ttctcctttt taaaatgtga taacgattta gggagtctct
    34381 taatcttatt gactaagaga ctgaaggttc tctaacttga gcttttatgt tactagctat
    34441 agatacagat ctttttgtca ttgatatcag ctagcgtgat gggaatctca taaagttgac
    34501 tgagcaggtc agcctgcatg atttgatcgg ttcttccctt gctaaagacc tggccgtcct
    34561 tgaaggcgac aatttcatct gcatactgac tggccatgtt gatatcgtgg aggacgatga
    34621 taatggtctt gccgagttcc tccaccagtc gtcgaagaat ctgcatcatg ctgacgcttt
    34681 gcttgatatc gagattgttg agtggttcgt ccagcaagat aaagtccgta tcctgggcca
    34741 gtaccatagc gataaagacg cgctggagtt gcccccctga caggctattg atgtagcggt
    34801 cttttaagtt ggtcagttcc aaatagttca gagtttctcg gattttttcc cagtcttctg
    34861 atctaagtcg acctcggctg tagggaaaac gtccaaaact gaccagttct tcaacagtca
    34921 atttggcttg gtaattgatt ttctgtttta ggatggttag ttcttgggcc agttcttgcg
    34981 aattccagct ctcgatttca cgtcctttga tactgagaac tccctgatct ttcttggtta
    35041 gtctgctcat gatggagagg agagtcgatt ttccagcacc atttggacca ataaaggctg
    35101 tcagtttttg aggactgact tcaagcgaaa tgccttgcaa aatatcctgt ttttgaatgg
    35161 atttgtcaat gttttccagt ttcactgacg agccctccta tatagtaaga taaagaataa
    35221 gaagccaccc acactctcaa tgatcatact gatacgaatt tccagtgcaa agactcgttc
    35281 aatcaaggct tgccccaagg ttaagctaat aaatccaacc agaatggcca ctataaagag
    35341 taacttgtgc tgatagtctt tgacaatcag gtaggtgagg ttggccagca taaagccgaa
    35401 gaaggccata ggtcctacca aggcagtggc cgttgaggtc aaaagcacga ttccccagag
    35461 gagctctttc tgttcttttt caacatcgag tcccaatatc tgagccgttt ctctttgcag
    35521 gtgcaagaca tctagaacga ctgcttttcg aaagaaaaag attgtcaaag cgaggatgat
    35581 cagagaaccg atggctagga tggaagtgtt aagatgttga aaggaggcaa aaagactatt
    35641 ttgcagttta tcgtattcgt ttggatccat taggacttga aggaaggtgc tgatatttcg
    35701 aaagagactt ctgagcgcta gacagatcag caggatgaag accaggtctt gcttcatcag
    35761 tgtcttcaag taaccttgta aggcgagaaa gaagaaggac tggacaagaa gtaagactag
    35821 gaattctaag ataggggatt tgccaagttg aagaaacttg ctttcaaaaa ccagtagtag
    35881 ggtttgtagt aggacgtaga aggattcaat tcccaaaata ctaggcgtca ggaagcgatt
    35941 ttccgtcagg gtttgaaaac taatggtcga aatcccagtc gcgatggcta ccaagagata
    36001 aacgatgatc ttttgggaac gcaacttcca agcaaaggct gacaagtgag tgatgggcca
    36061 aaagtagaga agacaagctc cgatggcaag aataatgaga atccagaaga gcttggtatg
    36121 tttgctttta gtctgcatct tttcgtcccc ctctccagag aagtaggata aagacgagac
    36181 taccgatgat tcctagcaag agactgacag acaactcata gggcctaatc agaactcggg
    36241 ataggatatc gcaagccaga actagattgg caccaaccag tgcgaccatg agtttggttt
    36301 gacttagatt atctccatag cgcttgcgaa caagattggg aacgataact ccgagaaatg
    36361 gtaagccacc cacggtaatc atggtgacgc ttgtcgttag cgccaccaga aagagggcca
    36421 gtttttcaag tagggagtag gaaatcccca aactctcgct ggtttctttc cccagattca
    36481 tgatggtgaa ggtttgggat aatttccaaa cggttatcag gatgatgagg cctaagaaga
    36541 gccactcata ctgatgggtc tgaatcatgg agaaggagcc ctgggtccag gcagtcatac
    36601 tctgaaccag attgaaacga taggcgataa cttctgtgac tgagccgata atcccgctat
    36661 agatgatccc aatcagaggc aacatccacc tttcctttac agtaaaaatg gtcataaagg
    36721 ctaggaagaa gagggtgaat acgatggatg aaacaaaagc gaagagcatc ttttgggtca
    36781 gactagccga tggaaagaca aaaaggctca gcaccattcc cagtttggcg gcttcagtcg
    36841 ttccaactgt actcggtgca gcaaactgat tttgggtaat agtctgcatg agaaggcctg
    36901 ccatactcat actagaggca gtcaggagaa tactgatagt tcttgggaga cgggactctt
    36961 gaaaaaggag ccaggtctgc tggtcgaaat caaatagctt tccccatgaa aaatcactgg
    37021 tcccaatgct aatagagaga aagactagga gtagaagtaa gccaattaaa taatgagaaa
    37081 gtttcatacc ccgtcctttc atgtagattt ggtatcgaaa gatatctgcg gatataaatg
    37141 taacattatt tttctaatct gtcaataaaa tttctgacaa tttaataaat ataacaagga
    37201 gagagcaaca agactttctc ctttgttatc ctattctaaa atgtttttac cttaatctga
    37261 taaaataata tcttcgaggg agtagctagc cgtccaatca agatattgtt tagcttttga
    37321 agcatctgct aggacactgg ctgggtcact agcacgtcga gcaacaatct cgtgtgggat
    37381 tttttaattt agtaattctt cagcagtttt aaagatttct ttgatagtat agcctttttt
    37441 agttcctaag ttaaagattt gagaagaact gtcttcttga aataggtagt tcattccttt
    37501 aacatgagcc tatgcaaggt ccaagacata aatgtaatct cgaatacatg aaccgtcacg
    37561 tgtatcgtag tcatctccaa atatttttaa gctatcattt tgtcccaatg cggtcttgtt
    37621 gatatttgga atgatgtgag ttggattttt cacacgcaga ccgtttgaag catccatttc
    37681 agccccagaa gcattaaagt aacggaaaat aacatatttc cagtcgtagc gattggccat
    37741 ccagtaaatc attcgttcgc ccatcagttt tgtctctgca taagggttga cagggtcgag
    37801 cagggtatct tcagtcaccg gcttgtcaat acagttattt ccatagagag aagcagtcga
    37861 agagaacatg attttttgaa tgccaacttc agataagact ttgagaactt ggttcatacc
    37921 agcaacgttg gcagtgaagt atttacttgg attttcaata ctttcgctca caacaatttc
    37981 acctgcacaa tggaggacag catcaatctg attttcttct aagtaagcct ttaaggcgct
    38041 agcatcataa acatccagtt ctttaaagct agcacgacta tccacagccg ctcgatttcc
    38101 tgtggagaga ttatcgagga catgcacctg atagccagca tttaaaagag ctttaacggt
    38161 atgggagcca atgtagctag tcccacctgt aacaagaatt gttttggtca tgattttttc
    38221 cttttctagt tttgtactat agta
//
LOCUS       DAVKQL010000014        36467 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVKQL010000014 DAVKQL010000000
VERSION     DAVKQL010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36467
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.014"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            <1..1080
                     /locus_tag="VW561_000634"
     CDS             <1..1080
                     /locus_tag="VW561_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase"
                     /protein_id="HEU9942930.1"
                     /translation="YFSYISVNNTLRICGIDYNDSNNYTKEQVLKNWDSMLRESILRV
                     YSEAGEANITLSSGFDSNYILYILANYTNSSINAFCIGGEKGINEIPEVTKIAKFYGI
                     NLLVDTIMSQDLEYFPDIVWRLEGSLFECGVILQYYLGRLLFQKGNTSILCGESADEI
                     MTFKYHSVNYNQFCNDKQKSVYFSYSDYPFYVTNSIVLKKNSLLLHSFGIHPRYPYKM
                     SEIVEMSKKISNLNDKKEFHKKNCELRFQDSVLDNINSVPGTTHLFSCLNIQTLVKII
                     LYIFRYNSCMKIFNFRDKELIFFNKIVNGLIQNIEENLEDDIERILKYLYICLFNEIF
                     IIKNKVNFFDDVEFNQTLSEFLDKL"
     gene            1092..1840
                     /locus_tag="VW561_000635"
                     /pseudo
     CDS             1092..1840
                     /locus_tag="VW561_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
     gene            1853..3025
                     /locus_tag="VW561_000636"
     CDS             1853..3025
                     /locus_tag="VW561_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEU9942931.1"
                     /translation="MNIAVIGLGHVGLAYALLFASKYKVVAYDIDSVKINNLKKGILP
                     SKNEELMKFFCENNLNITFFDTFSEIKNNIDYYIIALPTDYDEKIGSFNTYEIEQTVS
                     KILRVKPNGKIILKSTVPIGFSNKLKRLFDTKNIIFVPEFLREGCSIYDNLYPSRIVV
                     GDDTVEGRKIAELFLSISTHSTANIRNVMLVSPTEAEAIKLFSNTFLALRVAFFNELD
                     SFAERRSLNAEVVIKGVCLDPRIGNFYNNPSFGFGGYCLPKDTKQLKKEFIEINAPVI
                     EAIDISNTNRKQFIVKQILERKPKIVGIYKLGMKYNSDNYKESAILSIINELLIVGIK
                     ILVYEPNLNVSIDNVIFEKNFELFTKQSDLIVANRWDRGLEAYKDKVYTRGIWIRD"
     gene            3420..4283
                     /locus_tag="VW561_000637"
     CDS             3420..4283
                     /locus_tag="VW561_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU9942932.1"
                     /translation="MIEKMELGEFYKELRLARKLKQTDVACEGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMRIGRKVVNRFAHQDIAALEQLLEEV
                     EQEQMAQTYRRLNAIVIKNALHSLDKSYPLAEEDSEFLTTYLYAIESWTWFELYIFCN
                     TMPFLSNQDLVFLSTALIEKSKEFKELVHNRLYMKQGLLNILSELMERKLFSYIPIFE
                     AELESMLRPYDVFEKVLWQFLKKMSIFLQTKGSNQKEIENFIQSLQVLENPQLTALFK
                     LRLQQYKALID"
     gene            4569..4769
                     /locus_tag="VW561_000638"
     CDS             4569..4769
                     /locus_tag="VW561_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone VP1"
                     /protein_id="HEU9942933.1"
                     /translation="MLNLQFAETMELTEAELETVYGGEFGNNAVIPAGAWGGFGTPWS
                     ITNFWKKNFNDRPDFDSDRRRY"
     gene            4836..5501
                     /locus_tag="VW561_000639"
     CDS             4836..5501
                     /locus_tag="VW561_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9942934.1"
                     /translation="MKKIILHRYFIIVFLLVIADQKFSVLVLRSDLVTGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVVWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEEIIHRGLVMTVLEKGKKWGLDVL
                     GSAILFGVSHISNHGWVLTDFFVYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            5573..6799
                     /locus_tag="VW561_000640"
     CDS             5573..6799
                     /locus_tag="VW561_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9942935.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFTKFLLYSIVSLSLSGQSFSLL
                     LVTFICLLNIVSDTLSAFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(7195..7272)
                     /locus_tag="VW561_000641"
                     /pseudo
     CDS             complement(7195..7272)
                     /locus_tag="VW561_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            7343..7999
                     /locus_tag="VW561_000642"
                     /pseudo
     CDS             7343..7999
                     /locus_tag="VW561_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
     gene            7989..8312
                     /locus_tag="VW561_000643"
     CDS             7989..8312
                     /locus_tag="VW561_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEU9942936.1"
                     /translation="MVSKYLLLAVIFSGLVTWILRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAI
                     LRLVF"
     gene            8416..9246
                     /locus_tag="VW561_000644"
     CDS             8416..9246
                     /locus_tag="VW561_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9942937.1"
                     /translation="MKKIVKYSSLAALALVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPKPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            9400..10254
                     /locus_tag="VW561_000645"
     CDS             9400..10254
                     /locus_tag="VW561_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU9942938.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIGDTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEETS
                     DGLDQPVW"
     gene            10354..11727
                     /locus_tag="VW561_000646"
     CDS             10354..11727
                     /locus_tag="VW561_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU9942939.1"
                     /translation="MVFPSEQEQIEKFEKDHVVQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEAPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDVKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            11720..12781
                     /locus_tag="VW561_000647"
     CDS             11720..12781
                     /locus_tag="VW561_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEU9942940.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGGQ"
     gene            12783..13475
                     /locus_tag="VW561_000648"
     CDS             12783..13475
                     /locus_tag="VW561_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9942941.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(13505..14074)
                     /locus_tag="VW561_000649"
     CDS             complement(13505..14074)
                     /locus_tag="VW561_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU9942942.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADGVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPRTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            14209..14823
                     /locus_tag="VW561_000650"
     CDS             14209..14823
                     /locus_tag="VW561_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU9942943.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFISSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            14825..16471
                     /locus_tag="VW561_000651"
     CDS             14825..16471
                     /locus_tag="VW561_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9942944.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDR
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            16483..17769
                     /locus_tag="VW561_000652"
     CDS             16483..17769
                     /locus_tag="VW561_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9942945.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFAIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLSSFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            17821..18354
                     /locus_tag="VW561_000653"
     CDS             17821..18354
                     /locus_tag="VW561_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9942946.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELA"
     gene            18432..18902
                     /gene="nrdI"
                     /locus_tag="VW561_000654"
     CDS             18432..18902
                     /gene="nrdI"
                     /locus_tag="VW561_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEU9942947.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKRIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(19195..20457)
                     /locus_tag="VW561_000655"
     CDS             complement(19195..20457)
                     /locus_tag="VW561_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEU9942948.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
     gene            complement(20767..21225)
                     /locus_tag="VW561_000656"
     CDS             complement(20767..21225)
                     /locus_tag="VW561_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEU9942949.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(21222..21662)
                     /locus_tag="VW561_000657"
     CDS             complement(21222..21662)
                     /locus_tag="VW561_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9942950.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            22018..23967
                     /gene="mutL"
                     /locus_tag="VW561_000658"
     CDS             22018..23967
                     /gene="mutL"
                     /locus_tag="VW561_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEU9942951.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(24292..24759)
                     /locus_tag="VW561_000659"
     CDS             complement(24292..24759)
                     /locus_tag="VW561_000659"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEU9942952.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(24760..25965)
                     /locus_tag="VW561_000660"
     CDS             complement(24760..25965)
                     /locus_tag="VW561_000660"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEU9942953.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(25985..26620)
                     /gene="ribE"
                     /locus_tag="VW561_000661"
     CDS             complement(25985..26620)
                     /gene="ribE"
                     /locus_tag="VW561_000661"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEU9942954.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(26605..27705)
                     /gene="ribD"
                     /locus_tag="VW561_000662"
     CDS             complement(26605..27705)
                     /gene="ribD"
                     /locus_tag="VW561_000662"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEU9942955.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHYVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(27840..27954)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            28111..28704
                     /gene="ruvA"
                     /locus_tag="VW561_000663"
     CDS             28111..28704
                     /gene="ruvA"
                     /locus_tag="VW561_000663"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU9942956.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDGLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            28714..29277
                     /locus_tag="VW561_000664"
     CDS             28714..29277
                     /locus_tag="VW561_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU9942957.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTETELEAMLENPAIIRNRAKLFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            29274..29951
                     /locus_tag="VW561_000665"
     CDS             29274..29951
                     /locus_tag="VW561_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9942958.1"
                     /translation="MMSNKNKEILIFAILYTVLFMFDGVKLLASLMPSAIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVRL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(30106..31137)
                     /locus_tag="VW561_000666"
     CDS             complement(30106..31137)
                     /locus_tag="VW561_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEU9942959.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCPKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVIRFDYNK"
     gene            complement(31392..32075)
                     /locus_tag="VW561_000667"
     CDS             complement(31392..32075)
                     /locus_tag="VW561_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEU9942960.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(32087..33031)
                     /locus_tag="VW561_000668"
     CDS             complement(32087..33031)
                     /locus_tag="VW561_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU9942961.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            complement(33192..34358)
                     /locus_tag="VW561_000669"
     CDS             complement(33192..34358)
                     /locus_tag="VW561_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU9942962.1"
                     /translation="MKITEVIKKDGSKVYRANIYLGVDQVTGKKVKTKVTGRTQKEVK
                     QKATQEKIAFQKAGSTRQKASTIKNYQELANLWLESYKNTVKPNTQDNVRKLIDNHIL
                     PIFGAYKLDKLTTPLIQSIINELADKTNRGEKGAFLHYDKIHALNKRILQYGVTMQAI
                     PSNPARDVVLPRNTQKAKRKKVKHFENQELKKFLAYLDNLDSDRYRYYYETTLYKFLL
                     ATGCRINEALALSWSDIDLDNAVVHVTKTLNRDLEINSPKSKASYRDIDIDQATVSML
                     KQYKLRQTKEAWKIGQRESVVFSDFIHEYPSSSRLKRRLQTHFKRADVPNIGFHGFRH
                     THASLLLNSGIPYKELQYRLGHSTLSMTMDIYSHLSKENAKKAVSFYETALKAL"
     gene            complement(34691..35680)
                     /locus_tag="VW561_000670"
     CDS             complement(34691..35680)
                     /locus_tag="VW561_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3644 domain-containing protein"
                     /protein_id="HEU9942963.1"
                     /translation="MEDLSKRLVNKSIEAFIMGIEIYNKPTIKYRIEGFSFFICNAWE
                     LMLKAYIINNDGEESIYFKDSKDRTISLENAVETVFPDKHGSLRKNLIRIIELRNTST
                     HFITEDYEHIYAPLFQACVSNYILKMQEFHNEDITKQIAQNFLTLSVRIDQLNQEEIR
                     AKYSPKMAERILAEQDKIESEISTNNSDYAVPVETRFYITKRKQDADLFVKLDNTAET
                     SIGIVREIKDPNAIYPLTTREVIKIVNRYLKSKGILLTKIVGGEKQKKTFTTNDLQLF
                     NTFYDIKTNQRYCYHYKIGNRYGYSQILCDFIIEEIERNPETFVENLKKRQKK"
     gene            complement(35768..36403)
                     /locus_tag="VW561_000671"
     CDS             complement(35768..36403)
                     /locus_tag="VW561_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9942964.1"
                     /translation="MAKNSPQDIKNRLYFSARLNRLMKEQGKRQIDLHNDLGIPKSTL
                     TGYVKGRSMPNTENLQKLADYFKVEASDIDPRYFVIDPDVILKYEQPVIDFVTMFESL
                     SERKQKKFFEGVIKDLEHDNLPHDFLKEPSDLVTHLGSQLDEKLRIDKELKMLEKENA
                     MLEEEIRILKISNEYALIDPKDTEAINNFRNSHSEEDIIKVEELLNKKKQP"
BASE COUNT        10696 a         6600 c         7527 g        11644 t
ORIGIN      
        1 tatttctctt acatttctgt aaataatact cttcgtatat gtggtattga ttataatgat
       61 tcaaacaatt atactaaaga acaagtatta aagaattggg actctatgct acgtgaatct
      121 atattaagag tgtatagtga agcaggagag gctaatatta cactatcatc tggttttgat
      181 tcaaattata tattatatat attagcgaat tatacgaatt cttcaattaa tgcattttgc
      241 ataggaggag aaaaaggaat taatgagatt cctgaagtaa ctaaaattgc aaaattttat
      301 ggtataaatt tattagttga tactataatg agccaagatt tagaatattt tcccgatata
      361 gtatggagat tggaaggaag tctctttgaa tgtggtgtca tactgcagta ctatttaggc
      421 aggttgttgt tccaaaaagg gaacacaagt attctttgtg gcgagtcagc ggatgaaata
      481 atgactttta agtatcattc tgtgaactac aatcaatttt gtaatgataa acagaagtca
      541 gtatatttta gttattccga ctatccattt tatgttacaa attcaattgt tttaaagaaa
      601 aatagcctac ttttgcatag ttttggcatt catcctagat atccatataa aatgtctgag
      661 attgttgaaa tgtctaaaaa aatatcaaat ctaaacgata aaaaagaatt tcacaaaaag
      721 aactgtgagt tgaggtttca agatagtgtt cttgataaca taaatagtgt cccgggtaca
      781 actcatttat ttagttgttt aaatattcaa acattagtaa aaattatttt atatattttt
      841 cgatacaaca gttgtatgaa aatatttaat tttagagata aagagcttat cttttttaat
      901 aagattgtaa atgggctgat acaaaatatt gaggagaatt tagaggatga tattgaaagg
      961 atattgaaat atttatatat atgtttattt aacgaaatat tcattattaa gaataaggtg
     1021 aatttttttg atgatgtgga atttaatcag acacttagtg agtttttaga taaattgtag
     1081 agggaatggt tatgagtaac aatactaagt atatcttttt atctccacat ttagatgacg
     1141 caattttttc ctgtggtgat tatatttcaa aattaacttc agaaggagaa attgtacttg
     1201 ttattactat attttctggt taccctttga gccaacagct acagccctct gctaagcaat
     1261 ttcataagtt gtgtaacctt gggaaatatc cgattgaaga aaggaagaaa gaagacaggt
     1321 tagcttgtga acgtctacaa tgtgatttta gacacttatc gtactatgaa tgtctctata
     1381 ggaaagatag aaatggcaat tttctatacc gtcatatcta tagtgaatta aaaaatgagg
     1441 acacattaaa gaatgatatc ataaaagagc tgttaatgca tttagacgac aaatgtgtcg
     1501 tatattgtcc gctttcactt ggtgaccatg ttgctcatgt ttttgtgaat agtattggaa
     1561 gggcgcttga atttatgcgt tataaagtaa tatattatga ggacttccca tatgtaagtg
     1621 atagtagtat ggttagttat atgggaaaaa ctaaagaatt aaaaatgtat caagaggaac
     1681 ttgatgaaaa aattatattg atagaatatc atcaatattg tgctataaat ctcagatttt
     1741 gataatctgg aaatcagttg aaaaactttt aaacaatatt aaagagttgt atttaaggaa
     1801 tggggcagct tattctatta gattttggat taagaaataa ggaagtagtt ttatgaatat
     1861 agcagtaatt ggtttggggc atgttgggct ggcctacgcg ttactatttg catctaaata
     1921 taaagttgtt gcatatgata tagattctgt aaaaataaat aatttaaaaa agggcattct
     1981 tccatctaaa aatgaagagc ttatgaagtt tttttgcgag aataacttaa atattacttt
     2041 ttttgataca ttttctgaaa ttaaaaataa tattgattat tatattattg cgcttccgac
     2101 agattatgat gagaaaattg gtagttttaa tacatatgaa atcgaacaaa cggtatcgaa
     2161 gattctgagg gtaaaaccta atggaaagat tattttaaag tcaacagttc cgatcggctt
     2221 ttcaaacaaa ttaaaaaggc tgtttgatac aaaaaatatc atttttgtcc ctgaattttt
     2281 gagagaaggt tgttctatat atgataattt atatccaagt cgcatagttg ttggagatga
     2341 cacagttgaa ggaagaaaaa ttgcagagtt gttcctttcg attagtactc atagtactgc
     2401 caatattaga aatgttatgt tagtttctcc tactgaagca gaagcaatta agcttttttc
     2461 taacacattc ttagctctcc gtgttgcttt ttttaatgaa ctagattctt ttgctgagag
     2521 gagaagttta aatgctgaag ttgtaataaa aggtgtttgt ttagatccaa gaattggaaa
     2581 tttttataat aatccttctt ttggatttgg aggatattgt cttcccaaag atactaaaca
     2641 attaaaaaaa gaatttatag aaataaatgc cccagtgata gaagcgattg atatttcaaa
     2701 cacaaataga aaacagttca tagttaaaca aatattggaa cgaaagccaa agatagtagg
     2761 aatatataaa ttggggatga aatataattc agataattac aaagagtcag ctattttaag
     2821 tataattaat gaactattga ttgtcggtat aaaaatttta gtatatgaac caaatttaaa
     2881 cgttagtata gataacgtaa tttttgaaaa gaattttgaa ctatttacaa aacaaagtga
     2941 tctaattgtt gcaaatagat gggatagggg ccttgaagct tataaagata aagtttacac
     3001 aagggggatc tggataagag attaactatt gatattaaaa aggcaataat ttataaaatt
     3061 tgtacaaaaa aatgttaaat tttcacattt tcatagtata ctgtttaata gaaagtaggt
     3121 tctttgtaac cgctccatta taaaatcctt tcactcttcc ggtatactat taggaaaaac
     3181 ataactggag gatattttga gctcacacct catcgttcca gtggctgttc cccaagctcg
     3241 tgtactgccc aaaaaagtta gatattctat ttagaacgag gattgagttc tgtattgtac
     3301 aaggctcggt ccttttagag tcagcttaag gctggctttt tcaatcacca aagtgtcaga
     3361 atgttttgac aaatgaacgc aaataatgat ataatatgca aaagctagga ggtggtagga
     3421 tgattgaaaa aatggaactg ggggaatttt acaaggaatt acgtttggcg agaaagctta
     3481 agcagacaga tgtggcttgt gagggtctaa cagcttctca gttgtccaag tttgaactag
     3541 ggcagtctat gctgtctgcg gataagttga ttctagctat ccaaggtata aatgtgacct
     3601 ttgatgagtt tgggcacaag ctcaacaact atcaagaatc tccacatatg cgaatcggtc
     3661 gaaaggttgt gaatcgcttt gcccatcagg atatagctgc tttagagcag ctattggagg
     3721 aagtcgagca agaacagatg gcgcagacct atcgtcgttt gaatgctatt gtaattaaaa
     3781 acgcactcca ttccttagat aaaagctatc cgctagcaga ggaggatagc gagtttttga
     3841 ccacctatct ctatgctatc gagtcttgga cctggtttga actctatatt ttttgcaata
     3901 ctatgccctt cctgagcaat caagatttgg tatttttatc aaccgcttta attgagaaat
     3961 ctaaagaatt taaagagtta gtacacaatc gattgtatat gaagcaagga ctcttaaata
     4021 tcttatcaga acttatggag cgaaaacttt tctcttacat cccaatcttt gaagccgagt
     4081 tggagagtat gctccgtccg tatgatgttt ttgagaaagt gttgtggcag tttttaaaga
     4141 aaatgagtat ttttcttcaa accaagggaa gcaaccaaaa agagattgaa aattttatcc
     4201 aatctctgca agtattagaa aatccacaat taacagccct ttttaaacta cgcttacagc
     4261 aatacaaagc acttatcgat tagttagaaa tgggaaaaag ataataatct cagcaacgat
     4321 tgtcaataat gttaaaactt gtttcttcat atgacttccc tcctcattag taataacttt
     4381 attataccag ggaaataatc aaatatacca aaatcgcaaa tctgaaattt ctataagaaa
     4441 aaatatcaaa tatgcgattt tctaaaataa gccaattttc gtgttatact gtacttgtaa
     4501 agcacttgaa gcaaatccta ggtcgcagaa gtggtttaca aatgaagata attgaaggag
     4561 tgtaaaagat gttaaattta caatttgcag aaacaatgga attgacagaa gctgagttgg
     4621 agacagttta tggaggggaa tttgggaata atgctgttat cccagccggt gcttggggag
     4681 ggttcggtac accttggtca atcactaatt tctggaagaa aaattttaac gatagacctg
     4741 actttgattc tgaccgccgt cgttattaat atccaaagcc cttgcctttt cttgggtagg
     4801 ggctgtcttt atttcatagt ataggaggaa atagaatgaa aaagataatc ttacaccgct
     4861 actttattat agtttttcta ctagttattg ctgaccagaa gttcagtgtt ctagttttac
     4921 gcagcgacct tgttactggg ctatctgact ttgcctatta tctgtcggat atgatgttga
     4981 attttcttgt ggttttattt gctcttattg ctatgatttg gtcggggaaa tggcagaaaa
     5041 tcaatagtag aaagtttaaa ggatcctatc ttttctattc cttcctagct ctgcttgctt
     5101 ttgttgtttg gaatttcgtt accttttttc tattcccacc tactcgaaat gaaatttctt
     5161 atcaacatgc tgctccgact tttacagggg ctacagcatt tttgatgtat tttttctatc
     5221 ctgtgattgc aggtcccatt tttgaagaga taatccatcg gggattggtg atgacagttc
     5281 tggaaaaagg aaagaaatgg gggctagatg tacttggttc tgctatttta tttggggtct
     5341 cgcacattag taatcacggt tgggtcttga cagacttttt cgtctatatg ggtggcggac
     5401 ttatatttgc agtcttattt agaatgacaa agtccattta ttggcctatt gggctgcata
     5461 tagtctacaa cggcattggt cagcttttga tgttactgta attttggttg ttagtagtat
     5521 cgcggtctat ttctggggaa agattcccat ttgaacttaa gaaggagttg tgatgaaagt
     5581 atttcttcaa aatagagatt ttaggcaatt aactatcaac cagtggattt caacgcttgg
     5641 ggatacgatt ttttatctgg cctttttgaa ttatgtggca gatgcgtctt ttgccccttt
     5701 ggcgatttta cttatcacga tttcagaaac ccttccccaa gttctacaaa tctttctggg
     5761 agttttggca gattttcaac atcatcgtgt cttgaaatac acagtcatta gttttacaaa
     5821 atttttgctt tactctatag tgtccctatc gctttcaggg cagtcttttt ccttgttgct
     5881 agtaaccttt atttgtctgc ttaacatcgt gtctgataca ttgagtgctt tttcaggcgc
     5941 catgctcact ccgattttca ttagaattat tgggcaagac catctggcag aagctattgg
     6001 atttaaacag tcaactgtta gtttagtgaa aacaatcagt aatatcctag gaggagtctt
     6061 actaggcatt ctatccatcc agtttatttc cttactgaac gctcttacct ttttaatcgc
     6121 atttttaggt atcctcttca taaaaactga cctcttgaaa gtagaaaaaa cgattagcta
     6181 tcaagaagga ctctctgtaa aatccttttg ccagcatttg ctccaatcat cgaaattgat
     6241 atggaatatg aataaggtgc tcttggtttt gtttattatc tctactagtc aagcagtgat
     6301 aaatgttaca gttcctgtct ctactctatt tttgaggaat caaccctttt tgaatttaca
     6361 aacaggtcaa tctcttgcct tgctatccac ttttgaactg tcagccctga ttgtcggaag
     6421 ccttgtaagt ggctatctgc aacataccat ttccataaaa acagctctct atgcctcact
     6481 tgtcatccag ttgcttcttc tagtaggatt tgccacagtt cgttttgact ggattctcat
     6541 ctttagtacc ttggatgcct ttttcgcagg tgttctctct cctagattgc aagaactcgt
     6601 ttttaaacaa atacctgagg agtcaatggg agcagttcaa tcttctatcg gtgccattac
     6661 ggttgtttta cctagcttat ttacaatagc tttggtaacc attgctacta gctttggaac
     6721 tctggcagtt agctttgttt tattactatt tcttctagtt gcctttgtca tgcttctgaa
     6781 tattcgtgaa agtatttagt ggagaatttc tataatatat gtttgagact ggattgccag
     6841 tctttttatg atggtcaaaa tcaacagact ttttctcttt tctgaatgtg ttatagtaga
     6901 ctgaatctaa aatagtacga aacaattgct aaaacattta tagaaattaa ttttactttc
     6961 ccaatcgatt tgttctcatc ttatttcaat ctgctatata atagtccatg ctgtggctgg
     7021 attcttttca gcctacttat actcttcgaa actctcttca aaccacgtca gcttcacctt
     7081 gccgtaggta tggttactga cttcgtcagt tctatccaca acctcaaaac agtgttttga
     7141 gctgacttca tcagttctat ccacaacctc aaaacagtgt tttgagctga cttcatcagt
     7201 tctatccaca acctcaaaac ggtgttttga gcaacctgcg gctagtttcc tagtttgctc
     7261 tttgattttc attgagtatt attaagacaa tataaggaga aagatgaaag aaaaaggatt
     7321 ttaggagggg gcgcaggcag ccatgccaac ggctcttggc tatgtcagta tcggcctggc
     7381 ctgtgggact atcggtgcgc cctatgtgac acctgttgag atgggcttga tgagtctctt
     7441 tgtttatgct gggagtgccc agtttgccat gttggcactg attgtggttc aagctcctgt
     7501 ggcagctatt gctatgacgg tttttctaat caacttgcgt ctctttttgt tgagtttaca
     7561 cgcatcgact tatttccgtc ataccagtct ttggtacaat atcggtatgt ctagtatctt
     7621 gacagatgag acctatggcg ttttgatggg tgaattggcc catacagaca aggtaaatcc
     7681 tatgtggatg cacggaaaca atcttaacag ctatgtggct tggtttgtgg ggacagtagt
     7741 cggaacggct ctgggtggcc tgctaccaaa tccagaaatc tttggcttgg attttgccct
     7801 ggttgggatg tttattggta tttttgctta gcaatttcag attatgcaaa gacggattcc
     7861 tgtccgcaat ctgctcatta tcctagcagt tgttgcggtg tccttctttt tgctcttgac
     7921 agtggtgtct cagtcactag ctgttctgtt tgcgacgcta cttggttgta gcatgggggt
     7981 ggttttagat ggtcagtaag tatcttttat tagcagttat tttctctggc ttggtgactt
     8041 ggattctccg tatgattccc ttcatcttag tcaagtataa gggcttgcct gcaatcgttg
     8101 agcgtttttt gaagttcttg cccgtttcca ttatctttgc cttgattctt tcaagcgtag
     8161 tgacaggtaa ggttgggagc cttcctcaaa ttaaatggct agacttctta gccgtctttc
     8221 caacagcttg ggtagccttt cgctaccgca atctagtcgg aacagttctc tttggagtgg
     8281 tcttgattgc catcttgcgt ttggtctttt aaattaccca taaaaaaaac ttatcacaga
     8341 gatagatatc atataatggc gtaaatgctc tttttctgtt aagattataa ggtattctat
     8401 tttggaggaa atgacatgaa aaaaatcgtt aaatactcat ctcttgcagc ccttgctctt
     8461 gttgctgcag gtgtgcttgc ggcttgctca ggtggtgcta agaaagaagg agaagcagct
     8521 agcaagaaag aaatcatcgt tgcaaccaat ggatcaccaa agccatttat ctatgaagaa
     8581 aatggcgaat tgactggtta tgagattgaa gtcgttcgcg ctatctttaa agattctgac
     8641 aagtatgatg tcaagtttga aaagacagaa tggtcaggtg tctttgctgg tcttgacgct
     8701 gatcgttaca atatggctgt caacaatctt agctacacta aagaacgtgc ggagaaatac
     8761 ctctatgccg caccaattgc ccaaaatcct aatgtccttg tcgtgaagaa agatgactct
     8821 agtatcaagt ctctcgatga tatcggtgga aaatcgacgg aagtcgttca agccactaca
     8881 tcagctaagc agttagaagc atacaatgct gaacacacgg acaacccaac tatccttaac
     8941 tatactaagg cagacttcca acaaatcatg gtacgtttga gcgatggaca atttgactat
     9001 aagatttttg ataaaatcgg tgttgaaaca gtgatcaaga accaaggttt ggacaacttg
     9061 aaagttatcg aacttccaag cgaccaacaa ccgtacgttt acccacttct tgctcagggt
     9121 caagatgagt tgaaatcgtt tgtagacaaa cgcatcaaag aactttataa agatggaact
     9181 cttgaaaaat tgtctaaaca attcttcgga gacacttatc taccggcaga agctgatatt
     9241 aaataatttc ttgaaatcat ccattctgag taaggtgggt ggtttctcag tttttaggga
     9301 tatagtttta aactatatat ctgactatct aataacaatt tgtgaaatca aacaaattgt
     9361 gagatactag tacggtatta tttttaagga gaaagaatca tgaaaatcaa aaaatggctt
     9421 ggtctagcag cccttgctac agtcgcaggt ttggctcttg cagcttgcgg aaactcagaa
     9481 aagaaagcag acaatgcaac aactatcaaa atcgcaactg ttaaccgtag cggttctgaa
     9541 gaaaaacgtt gggacaaaat ccaagaattg gttaaaaaag acggaattac cttggaattt
     9601 acagagttca cagactactc acaaccaaac aaagcaactg ctgatggcga agtagatttg
     9661 aacgctttcc aacactataa cttcttgaac aactggaaca aagaaaacgg aaaagacctt
     9721 gtagcgattg gagatactta catctctcca atccgccttt actcaggttt gaatggaagt
     9781 gccaacaagt acactaaagt agaagacatc ccagcaaacg gagaaatcgc tgtaccgaat
     9841 gacgctacaa acgaaagccg tgcgctttac ttgcttcaat cagctggctt gattaaattg
     9901 gatgtttctg gaactgctct tgcaacagtt gccaacatca aagaaaatcc aaagaacttg
     9961 aaaatcactg aattggacgc tagccaaaca gctcgttcat tgtcatcagt tgacgctgcc
    10021 gttgtaaaca ataccttcgt tacagaagca aaattggact acaagaaagc acttttcaaa
    10081 gaacaagctg atgaaaactc aaaacaatgg tacaacatca ttgttgcgaa aaaagattgg
    10141 gaaacatcac ctaaggctga tgctatcaag aaagtaatcg cagcttacca cacagatgac
    10201 gtgaaaaaag ttatcgaaga aacatcagac ggtttggatc aaccagtttg gtaataagaa
    10261 acagggaggt gggagagaga attccacctc ttgcttttgt atagagcacg gagtgtaaag
    10321 aagattattc gttcatagaa aggtagagag aatatggttt ttcctagcga acaagaacag
    10381 attgaaaaat ttgaaaagga tcatgtagtc caacattatt ttgaggtttt gcgtaccttg
    10441 atttctaaga aatcagtctt tgcccagcag gttggactca aggaagtcgc aaattatctg
    10501 ggtgagattt tcaagcgtgt tggagctgaa gtggagattg atgagagcta tacagcgccc
    10561 tttgtcatgg cacatttcaa gagttcgcgt ccagatgcca agaccttgat tttctataac
    10621 cactatgaca ctgtgccagc ggatggggat caggtctgga cagaggctcc ttttacgctt
    10681 tcggtccgca atggcttcat gtatgggcgt ggggttgatg acgacaaggg tcatatcaca
    10741 gctcgcttga gtgctttgag aaaatatatg cagcaccatg atgatttacc tgtcaatatc
    10801 agctttatca tggagggagc ggaggaatcg gcttcaacag acctagataa gtatttggaa
    10861 aagcatgcag acaaactccg tggggcggat ttgttggtct gggaacaagg gaccaaaaat
    10921 gccttggaac agctggaaat ttctggtggc aataagggga ttgtgacctt tgatgtcaag
    10981 gtaaaaagcg ctgatgtgga tatccactcg agttatggtg gtgttgtgga atcagctcct
    11041 tggtatctcc tccaagcctt acagtctctt cgtgctgcgg atggccgtat cttggttgaa
    11101 ggcttgtacg aagaagtaca agagcccaat gaacgagaaa tggccttgct agaaacttat
    11161 ggtcaacgaa acccagagga agttagtcgg atttatggat tggagttgcc tctcttacag
    11221 gaggagcgga tggcctttct aaaacgtttc tttttcgagc cagcgcttaa tatcgaagga
    11281 atccagtctg gttatcaagg tcagggtgtt aagactattt tgcctgcaga agccagtgcc
    11341 aagctagagg ttcgtctggt tccgggtcta gaaccgcatg atgttctgga aaaaattcgg
    11401 aaacagctag acaaaaatgg ctttgataag gtagaattat actatacctt gggagagatg
    11461 agctatcgaa gcgatatgag cgcaccagcc attctcaatg tgatcgagtt ggccaagaaa
    11521 ttctatccac agggcgtttc agtcttgccg acgacagcgg ggacaggacc tatgcatacg
    11581 gtctttgatg ccctagaggt gccaatggtt gcattcggtc taggaaatgc caatagccga
    11641 gaccacggtg gagatgaaaa tgtgcgaatc gctgattatt atacccatat cgaattagta
    11701 gaggagctga ttagaagcta tgagtagaga tattatcaag ttagatcaga tcgatgtgac
    11761 ttttcaccaa aagaagagaa ccatcacagc ggttaaggat gtgaccattc acatccaaga
    11821 aggggatatc tacggaatcg ttggatattc tggagcaggg aaatcaaccc ttgtacgggt
    11881 gattaacctc ttgcaaaaac catctgcagg gaaaattacc attgacgacg atgtgatttt
    11941 tgatggtaag gtgaccttga cggcagagca gttgcgtcgt aaacgtcaag atatcgggat
    12001 gattttccag cattttaacc tgatgagcca aaagacagca gaggagaatg tagcctttgc
    12061 ccttaaacac tctggactca gcaaggaaga aaagaaggct aaagtagcta agttgttgga
    12121 cttggttggt ttggcagatc gtgctgaaaa ctacccttca caactatctg gagggcaaaa
    12181 acagcgtgtg gcaattgcgc gtgccttggc caatgatcca aaaatcttga tttcagacga
    12241 gtcaacttct gcccttgacc ctaagacaac caagcagatt ttggccttgt tgcaagattt
    12301 gaaccaaaaa ttaggattga cagttgtctt gattacgcat gaaatgcaga ttgtcaaaga
    12361 cattgccaac cgtgtggcgg ttatgcagga tgggcatttg attgaagagg gaagtgtcct
    12421 tgaaatcttc tcaaacccta aacaaccttt gactcaagac tttatctcaa cagccacagg
    12481 tattgacgaa gccatggtca aaatcgagaa gcaagaaatc gtggaacact tgtctgaaaa
    12541 cagtctcttg gtgcaactca agtacgctgg agcttcaaca gacgagccac ttttgaatga
    12601 attgtacaag cattaccaag taatggctaa tattctctat gggaatatcg aaattctcga
    12661 tggtactcct gttggagaat tggtggtggt cttgtcaggt gaaaaagcag cgttggcagg
    12721 tgcccaagaa gccattcgtc aagcaggtgt acaactaaaa gtattgaagg gaggacagta
    12781 agatggaatc attgattcaa acctatttac caaatgtcta taaaatgggt tgggctggtc
    12841 aggcaggctg gggaacggct atctacttaa ctctttatat gacagttctt tccttcatta
    12901 tcggaggctt cttggggcta gtggcaggtc tctttctcgt cttgacagcg ccaggtggtg
    12961 tcttggagaa taaagtcgta ttctggattt tagacaaaat tacctcaatt tttcgtgcgg
    13021 ttccctttat catcctcttg gcaatcttgt caccactttc tcacttgatt gttaaaacaa
    13081 gtatcgggcc aaatgcagcc cttgtcccac tttcttttgc agtctttgcc ttctttgccc
    13141 gtcaggtgca ggttgtcttg gctgaattgg atggcggtgt cattgaggcg gctcaagcga
    13201 gcggagcgac tttctgggac atcgtgggtg tttacctatc agaaggtctt ccagatttga
    13261 tccgtgtgac gactgtgacc ttgatttccc ttgttgggga aacagctatg gccggtgcgg
    13321 ttggagctgg tggtatcggt aacgtagcca tcgcttatgg atttaaccgc tacaatcacg
    13381 atgtgaccat cttggcaacc attattatca ttttgattat ctttgcaatc caattcttgg
    13441 gagatttctt gactaagaaa ttgagccata aataaaaaag agccgcgtgg ctctttttaa
    13501 ctgatcagat tttctgtgca aattttttac tcaaggcttg tccaatcaag gcacccacta
    13561 gggctccgat gacaatactt gcgataaata gaaggacagt tctagggttt ggagcgacca
    13621 tgatacggtc gatatattct tgggattttc ctcttgccag aagagtagcc atataggctt
    13681 tgggcgcaat ccacataagc aagattggtc ctgttgtact aaaggcgaaa ataatgaaag
    13741 aaaggaagtt ctttgttttg tccttgtatt ttcctaaatg agctactcca tctgctagga
    13801 ggccacagat aattccagga aggaaggaac cagcaccgtg tttagttccc aagaaaaaga
    13861 gggcaatgac aaggccgata gtggtaatgg ctccaaagcg cggaactttt gcgactagga
    13921 tcatgtagac gctaccgccg acaagggcag taaaggcagg cgcataaaac atgtttccag
    13981 tttggtcaaa gagattgccc aaaaggacac caatccccat gcagaggaag taaagaactg
    14041 caaaaagcag tgtagttaag atagatttct tcatgaattg tctcctgata attttttcac
    14101 aattctcatt ttaccatggt ttggtgggat tgtaaatgga tactagaagt ttttgaaaac
    14161 gcttgaaata tgatataatg gtttcatagt tatttttagg ggaatatcat gggtagattt
    14221 ttagactttg tctttaatcg tttcttttta gggatgattg cgacagcctt cttttggcta
    14281 ttaactttag caggagggat tatccttggt ctagcgccgg ctagtgccac cttgatgagc
    14341 ttatatgcag aacatggtta tagctttcgg gaatacagtt tgaaggaggc ttggtctctt
    14401 tacaagcaaa attttatctc aagcaacctg attttctata gctttttagg tgtgggtcta
    14461 gttttgacct atggtttgta tctcttggtg caattacctc atcagaccat tgttcatttg
    14521 attgcgaccc ttttgaatgt cctagtagtt gccctgatct ttttggctta tacagtatct
    14581 ttaaaattac aagtttattt tgccttgtcc tatcgaaata gtctcaaatt atccttgatt
    14641 ggcatcttta tgagtctagc agctgtggct aaggttctcc ttgggactgt gctacttgta
    14701 gcaattggtt attatatgcc tgccctgcta ttttttgtag gaattgggat gtggcatttc
    14761 tttatcagtg atatgttgga acctgtctat gaaatcatcc atgaaaaatt ggcgacaaaa
    14821 tagaatgaag cagttttggc tacatacgct tctaagaacc tatagttcag tgatgatcat
    14881 tatcattgcg agttttgcaa tcttactctc ttacgctgac tgggattcac gtgaaaagga
    14941 agcccagaga gtagcccagc gtgtaactgc tagaacagtg agtgaaattg aatattacca
    15001 tagagagtca acccagatag ctcaggcttt agttgaaaat caagcgcgta tcgagggaat
    15061 ctataaatac tttagcctta gcatgccaga ctatttttac tggcaattag agcggaaagc
    15121 ttcgccttat atatcagtct ctctgtatga aaatgttgat gacctctatg ttcgaaatga
    15181 ttttgtaact ggggtggcca ttgcttttca agattacaag gaagtctatg tttctactaa
    15241 agacaaacgt agtggagaaa aaatcagggc tgaggatttc aaaccagcag gaaatagttt
    15301 tgccattccg gtgtcagatc cagtgtcaga tcaagattta ggagtgattt acatctcctt
    15361 ggatcctgct gttttatacc atgccattga taatactaga ggtcatactc cgatggcagt
    15421 aacagtgacc tcaccttttg atacggagat ttttcatatt ggtgagacag ttgataggga
    15481 gagtgaaaat tggctagttg gcttaacttc tcatggatat caggttcagg tggcagttcc
    15541 taaaaacttt gttttacaag gaacagtgac tagctctgct ttgattgtgg gtttgagcct
    15601 tctctttatt gtcattcttt atctgacttt gaggcagact tttgctaatt accaaaagca
    15661 ggtagtggat ttagtggaat ccattcaagt cattgctcaa ggcgaagagg ggcgtcggat
    15721 tgacatttcc gaaaaagatc aggaattact cctaatcgcg gagacgacca atgatatgtt
    15781 ggatcgattg gaaaagaata tccatgatat ttaccagtta gagcttagtc aaaaagatgc
    15841 caatatgcga gccttgcagg cgcaaatcaa tcctcatttt atgtataata cgctggagtt
    15901 cttgcgcatg tatgcagtta tgcagagtca agatgagttg gcagatatca tttatgaatt
    15961 cagtagtctc ttgcgtaaca atatttccga cgaaagagag accctcctca aacaggaatt
    16021 agaattttgc cgtaaataca gctatctctg catggttcgc tatcccaagt ccattgccta
    16081 tggtttcaag atagatccag agttagagaa tatgaagatt cccaagttta ccttgcaacc
    16141 gctggtagaa aactatttcg cgcatggtgt tgaccacagg cggacagata atgtgattag
    16201 catcaaggct cttaaacagg atggttttgt ggaaattttg gtggtcgata atggtagagg
    16261 aatgtcggct gaaaagttgg caaatatccg agaaaaatta agtcagagat attttgaaca
    16321 ccaagccagc tacagtgatc aaaggcagtc tatcgggatt gtcaatgtac acgagcgttt
    16381 tgtgctctat tttggagacc gctatgccat tacgatagag tctgcagagc aagccggtgt
    16441 tcagtatcgt attacaattc aagatgagta gaaagggaga aaatgtataa agtattatta
    16501 gtagatgatg agtacatggt gacagaaggt ctgaagcgtt tgattccctt tgataagtgg
    16561 gatatggagg tcgtcgcaac agccagtcat gccgatgaag ctctagaata tgttcaggaa
    16621 aatcctgtcg atgtcatcat ttccgatgtc aatatgccag acaaaacagg gcttgatatg
    16681 attcgggaga tgaaagagat cttaccagat gctgcctata tcctgctctc aggttatcag
    16741 gagtttgatt atgtaaaaag agcaatgaac cttagtgtgg tggactattt ggtcaagcct
    16801 gttgataagg tagagctggg aaatctgctg gagaagattg caggtcagct cggcgagaga
    16861 gggaagaaaa gtcagactct tagtcaagaa ttagacgagg ctggatttgt tagttattta
    16921 ggggataagg agaattggtg gataggtcta tccaaggaaa aacaaggttc cttcgccatt
    16981 ccctactatg tcttgggcca agactggcag attttcattt ctgaccaacc cctagatggt
    17041 ttagtcgtta caccttttga agctccttat caagaacact ttgaacgctg gaagctgaat
    17101 gctgagaaaa ccctctttta cggttctgta aatctgcagc agtctgagag tctctttgcc
    17161 tattacgaac cgatttatag ggttatcatt cagggaaatc tcaatcaaat cgtagaagag
    17221 ttaaatctct tggagaaggt agttcttgaa aatacgccgc gtgttccgat tactaaacag
    17281 ctttttattc agtttgtcat ggatgttttc catttatttg aacatctcaa agctgatgac
    17341 ctgacagaca tcgtcaaaac cattcatgct attcaatcct tcgatgaatt ggttccttat
    17401 atcaaggaaa ctctgagcag ctttttcggt caataccgta tgaatgaaaa tgtggtcagt
    17461 gtgctggaag tcattggtcg tgattaccaa aaagagcttt ccctcaagga tatcagtaag
    17521 gctctcttta tcaatcctgt ctatctaggg cagttgatta agcgtgaaac cgattcgact
    17581 tttgcagagt tactaaacaa acaacgtatt aaggctgccc aacaactttt gctttcaact
    17641 agtgacagca tcgaagatat ttgttatgct gttggttaca gtaaccttgg atatttctat
    17701 aaagtttttc gaaaattgtg cggaaaatcg ccaaaagcct accgaaaaca ggtagaaact
    17761 atactataag atttgtattc ctttacaaaa ggtgctataa tattatcagg aggttctatc
    17821 atgaaaaaga aaccaattta tctatgggtc ttgctaatct tgtctgccct tatttcagtt
    17881 ccatctctgt ttggaattgt gagtcccttg cctagtaaag aagcccttcg tgctgctcag
    17941 aaacaagttg caggggtcaa tgctcagcaa ttagaagacc agcttaatta tacctataga
    18001 gtagcagaag catctcattc tatttttaat gttgctttga ttgtgctatc tactatttta
    18061 gtggtcgtag cgattgtctt ccttgttcgt aaaaatttgc aatatgcaaa ctatacttac
    18121 gttggttatg ttttgttagc tattattggt tcgatttatg gctatgttgg tttacaagac
    18181 gctgtgcagt tggttcaaga tgagagcatg cgtttgacag tgagtattgg ttcaaaagct
    18241 gttagcattt tctatatcgt catcaatgtt ctcttcctag cccttgtctt ctataagatg
    18301 tggcgacaac agaaagcctt ggcagaggaa gaggaaacag aagagcttgc ctaagtattg
    18361 acaaaaaaga actatcaaga tacaatcttg gtagttgttt tttgattata gataaaatca
    18421 tggaggtaca tatgaagaca atttctttag tttatattag tttgagtggc aacactgaaa
    18481 gttttgtgac gcgcttgaaa gactatctct tgtcccagta caaaaggatt gaggttcaaa
    18541 agattcatat caaggatttg gtcaaggaag gcaaaaattt ctatgaaatg gaccatccct
    18601 atgtcgcttt tttgccgacc tatctcgaag gtgggaatgg cgtggataac ggagatgttg
    18661 agattttgac gacaccggtg ggagatttta ttgcctatgg taacaatgct agtaagtgtt
    18721 ttggtgtggt tgggtcagga aatcgtaact ttaataacca atactgccta acagccaagc
    18781 aatacagtca acgctttggc ttccctgtat tggctgactt tgaaatgcga ggcatgctgg
    18841 aagatatcaa acatgtcgca gctattatcg cagatttgta tgagttggaa aaggagaatt
    18901 aatactcaat gaaaatcaaa gagcaaacta ggaagctagt cgcaggttgc tcaaagcaca
    18961 gctttgaggt tgcagataga gctgacgtgg tttgaagaga ttctcgaata gtataaagtt
    19021 cttataagag gtggttgaag ttgttcaact gttttttgag tataaacagt ctttaagaac
    19081 gtaaaaaagc ataagttcag gtctttaaaa ccttgttctt atgcttttta tcatagaaat
    19141 atttgctgaa ttttctcttg aaatgaaacc ttatcttcat ctcgcgcttg tcccttaccc
    19201 tgcttttccg attgcgagtg cgtagataaa gaagatggcg aagccaaaga ggaaaatcaa
    19261 tcccgcaatg gcaaggtgtt tgagccaaga aggaagattt ttgttttcac gcgttaccaa
    19321 ggcgtaattc aaaagagcaa agaaaggtgt tgtcaggaaa gatccaatca tggcaaagcg
    19381 gagcatggtt gaaacctgac cagcgaagaa cttgataatg acgataccga tgatagcagt
    19441 gatggtcatc cagatgttca aagatttacg attgtcctct ttttgactga ttagcagtcg
    19501 gagagattcc tgattaacgc gagaatagcc atcgataaca gttataactg ttccaaagat
    19561 acagaggaag gcaataaagg taatcaagta acgggaccat tcgccaagaa cagaggcata
    19621 catgcccacg aattgagaga tgtatttggc tgaagcagct tcaaccgcct gccctgtagg
    19681 atactgaatc agtgctccca gtgccacaaa gaagacggct aggatagctg ttccaatata
    19741 accagtgtta aagtcaaaca gagcgtcttc tgtgttaaag ttgacggtct ttctcttttc
    19801 agctgaccaa agtgaattga tggctgaaat ttcaatagga gccggcatcc atcctaggag
    19861 ggagacgatg aagggcagag ctgccatttg ccaaggtgtc ttctcgacaa aatcagaact
    19921 gtattctgga tgcttgaccg ctgctatgat aactgccaga acagtcgcaa tggttaaggc
    19981 agacataatc catttgacca tgccgtctaa aagtttgtag cctccaaaga gtagcatagc
    20041 ccaaatgatt gcaacgagaa tgagggacca ctgagtaatg ctaagtccaa tcattgggaa
    20101 ggcactggcg atgatagctg agcacagaat ggcaacacca gccgtgttga ccatagccga
    20161 aaagacattg agaataaaga aaatccagag atagagtttt cctttttcgg cataaccttc
    20221 aaccaaagtc tttccagtat cagctgtgta ttcagcacca aaacggaaaa atggatattt
    20281 aaagacattg gctaagatga ccaagagaag tagagaccaa ccgtaagaac cgccagcttg
    20341 agttgaggat acaatgtggg aacctccaac agcggcagtt gccattagga ttccaggtcc
    20401 cattgctttt agcttgcttg cccaggttga ttgatttaag gaaatagctt gcgacatgat
    20461 aaatactcct ttttgaattt tcagaataat ctgtatatgc aatctatatt ctacaaaaaa
    20521 ttttggaaaa atgcaagaat attttgaaaa aagatagaaa atttacaaag aaaatctgaa
    20581 ataactgtta tgatatagca gattgaaact agaatagtac acttttgctt ctaaaacatt
    20641 gttagaaatc gatttgactg tcctgattga tttatcctgt tcttatttca tttcactata
    20701 gtaaggtgca gcaggataaa gcaaaaccga gaagtttctt tctcggtttt gcttattatg
    20761 aggttcctat tttctatgct ccaagagttg attgatgtga tctacttttt tagcctctct
    20821 tttatagaga atagcccaga tgatgagata gacaatcgca aactcgataa tgagctggag
    20881 gtagaaaatc cagtggaagg ggaaccaacc agcaagagtt gctagtggga caaagccagc
    20941 cagcataagg aagaaatgag tcagagtggc acgaagcatg ctccagtcac ggctgaataa
    21001 gcggttgcca aagttgaaga gcataccgat ggctgcccag ataagtgtgc agtagagcaa
    21061 gaccagggca ccgtgaacct gatgctgatc catcacttgg ccgatgagag agtagggatt
    21121 tagtggtgcg taggtatttg gtgcataaat gagtgaaaag aggatggaga ggatgaggcc
    21181 gataagaaca ccggcagctg catcgtgaaa tacttgtttt ttcatagttc taatttctcc
    21241 ttgatggttt ttagataacg gcgtgaagag taggtgaagc tttcgttttt caagaaaatt
    21301 tctaccagac cgtttggcgt gagcttgagg tgagagatgg aatcgatatt gatgatttct
    21361 gattgggaaa tttggataaa attggttggc aagagtttaa gaacctgata gagtcgcaaa
    21421 tcaatgctgt aggtctgact cgcggtttct gctagaacct tccgattctc gatatagaag
    21481 cgctgaatct tgccaatctc aactagatag acctgatcat cgatttttcc tttgattttt
    21541 tctctttggt ccagattttc tgcgaactcg atgactttct ggactttttc ggtttcttga
    21601 ggtgcttgga caatcagctt ttcctcctcg taagtctcac taatctgtag ttctactttc
    21661 ataaatttct ctccttgttt ttcatacaaa attaatctca cttcttgtac acctttatta
    21721 atactcttca aaaatctctt caaaccacgt cagcgtcgtc ttgccgtagg atagttgttg
    21781 actttgtcag ttttatctac aacctcaaaa cagtgttttg agctgtctgc gtctagcttt
    21841 ctagtttgct ctttgatttt tattgagtat accactattt tactccctct ggcaagggac
    21901 tttgtctatg tggagggatt gggctcctat gtggtggagt ttttctgttc tttctgaaat
    21961 atggtataat agcactaatt aatttctagg aaaatagata cagaaagggg ctgaaagatg
    22021 tctcatatta ttgaattgcc agagatgctg gcaaaccaaa tcgcagctgg ggaggtcatt
    22081 gaacgtcctg ccagtgtggt taaagagttg gtagaaaatg ccattgacgc gggctctagt
    22141 cagattatca ttgagattga ggaagctggt ctcaagaagg ttcaaatcac ggataacggt
    22201 catggaattg cccacgatga ggtggagttg gccctgcgtc gccatgcgac cagtaagata
    22261 aaaaatcaag cagatctctt tcggattcgg acgcttggtt ttcgtggtga agccttgcct
    22321 tctattgcgt ctgttagtgt cttgactctg ttaacggcgg tggatggtgc tagtcatgga
    22381 accaagttag tcgcgcgtgg gggtgaagtt gaggaagtca tcccagcgac tagtcctgtg
    22441 ggaaccaagg tttgtgtgga ggatctcttt ttcaacacgc ctgcccgtct caagtatatg
    22501 aagagccagc aagcggagtt gtctcatatc attgatattg tcaaccgtct gggcttggcc
    22561 catcctgaga tttcttttag cttgattagt gatggcaagg aaatgacgcg gacagcaggg
    22621 actggtcaat tgcgccaagc aatcgcaggg atttacggtt tggtcagtgc caagaagatg
    22681 attgaaattg agaactctga cctagatttc gaaatttcag gttttgtgtc tttacctgag
    22741 ttgactcggg ctaaccgcaa ttatatcagc ctcttcatca atggccgtta tatcaagaac
    22801 ttcctgctca atcgtgctat tttggatggt ttcggaagca agcttatggt tggacgtttt
    22861 ccactggctg tcattcacat ccatatcgac ccttatctag cggatgtcaa tgtgcatcca
    22921 actaagcaag aggtgcggat ttccaaggaa aaagaactga tgaccctggt ttcagaagct
    22981 attgcaaata gtctcaagga acaaaccttg attccagatg ccttggaaaa tcttgccaaa
    23041 tcgaccgtgc gcaatcgtga gaaggtggag caaactattc tcccactcaa agaaaatacg
    23101 ctctactatg agaaaactga gccgtcaaga cctagtcaaa ctgaagtagc tgattatcag
    23161 gtagaattga ctgatgaagg gcaggatttg accctgtttg ccaaggaaac cttggaccga
    23221 ttgaccaagc cagcaaaact gcattttgca gagagaaagc ctgctaacta cgaccagcta
    23281 gaccatccag agttagatct tgctagcatc gataaggctt atgacaaact ggagcgagaa
    23341 gaagcatcca gcttcccaga gttggagttt ttcggacaaa tgcacgggac ttatctcttt
    23401 gcccaagggc gagatggact ttacatcata gatcagcacg ctgctcagga acgggtcaag
    23461 tacgaggagt accgtgaaag cattggcaat gttgaccaga gtcagcagca actcctagtg
    23521 ccctatatct ttgaatttcc tgcggatgat gccctgcgtc tcaaggaaag aatgcctctc
    23581 ttagaggaag tgggcgtctt tctagcagag tacggagaaa atcaatttat tctacgtgaa
    23641 catcctattt ggatggcaga agaagagatt gaatcaggca tctatgagat gtgcgacatg
    23701 ctccttttga ccaaggaagt ttctatcaag aaataccgag cagagctggc tatcatgatg
    23761 tcttgcaagc gatctatcaa ggccaatcat cgtattgatg atcattcagc tagacaactc
    23821 ctctatcagc tttctcaatg tgacaatccc tataactgtc ctcacggacg tcctgttttg
    23881 gtgcatttta ccaagtcgga tatggaaaag atgttccgac gtattcagga aaatcacacc
    23941 agtctccgtg agttggggaa atattaaaag tataaaaaag tctgggaaaa attttcaaaa
    24001 tcagaaaaac gcataaaatc aggtgttcaa aaaccttgat tttatgcgtt ttatcatgga
    24061 aatagttact tcatgttttc ctaattcttt tcgaaactct ttttaaacca cgtcagtttt
    24121 atcagtaatc tcaaaacagt gttttgagct aattttgcca gttttgtctg taacatcgaa
    24181 gttgtgtttt accactctgc gactggtttc ctagtttgct ctatgatttt cacagagtat
    24241 taaattgcga ttttgccaag tttctttatt cgtctaaaag tagagtctgt tctatgcgtc
    24301 taatgtacga attaggttga ccatttcaat agctccttgt gcacactcag aacccttatt
    24361 tcctgctttg gtaccagctc gttctatggc ttgttcaatt gtatctgtcg ttagcacacc
    24421 aaacataaca ggaatttcgc tatttaaact gatttgggcg attcccttag atacctcgct
    24481 acatacataa tcataatgac ttgtattccc tctaatgaca gctcccaagc agataattgc
    24541 atcatatttt ttactttttg ccatttttga tgcaatcagt ggtatttcaa aagctcctgg
    24601 aacccaggct acctctatat ctttctcgtt tacattctct cttttgagat tatctagtgc
    24661 tccagataat aattttgaag ttataaattc attaaatctc gctacaacaa tacctatttt
    24721 aatattgttt gctactaaat taccttcata agtgttcatt tatttttcct ccatatttaa
    24781 aatgtgaccc attcgatttt tctttgtttc taaataaaaa ctatcgtaag gattggcttc
    24841 tatttcgatt gatattctac tggaaatggt aattccatat ttttctaact gttcaacctt
    24901 gtcaggatta tttgtcagta aatgaagtga ctgaagtccc agatctttaa gcatttgtgc
    24961 tccaatatga tattctctta aatcaccttc aaagcctaat gcaagattgg catcaagcgt
    25021 atccatgcct tgatcttgta aatgataggc ttttaattta ttgataagtc caattcctcg
    25081 tccctcctgt cgcaagtaaa gtaagacacc cgaaccattc tcaacaatca ttttcatagc
    25141 tttatcgaat tgctgtccac aatcgcaacg taaagagcct aaaacatctc ctgttaaaca
    25201 ttcggagtgg acccgacata atacattggc ttcatcctct atgtttccca taataagagc
    25261 aagatgatgt tccccattta gtttatctat atagctaatt gctttgaaat taccgtatct
    25321 agtaggcata ttgacagttg aaactcgttc taccagctga tcatatactt ttctatattc
    25381 ttgtaattct ttgatggtaa ttagtggaat gttgtgtttc ttcgaaaact gaattaaatc
    25441 atctgttctc atcattttgc catcatgatt cattatttca caacataggc cacactcttt
    25501 tagtccagct aattttaata aatcaacagt tgcttctgtg tgtccatttc tttctagaac
    25561 accacctttt tttgcaatta aaggaaacat gtgtcctggc ctgcgaaaat cagagggtgt
    25621 tatatcttca gctacacaca tacgtgcggt cagtcctctt tcctcggcag aaatacctgt
    25681 ggtcgtttct ttataatcaa ttgaaactgt aaaagcagtc ttatgattat ctgtattgtt
    25741 ttcaaccata ggtgaaagca ttaattgatt agctaaactt tcgctcatag gcatacaaat
    25801 taatcctttg gcataagtag ccataaaatt aacattttct gttgtagctg cttgtgcaga
    25861 acaaattaag tctccttcat tttctctatc cttgtcgtct ataacaagaa caagtcgtcc
    25921 cttctgcaat gcttctaatg cttcttgtat ttttcgatat tccattgact gattatcctt
    25981 tctgctaaaa tccattttga tataatagtt ccttagatat ttctgatttt ggagagttat
    26041 ccatcagttt ttgcacatat ttacctaaga tatcattttc aagatttact gtactcccga
    26101 cttgtttact cttaagaatg gtttgttcca aggtatgagg gataacagat actgaaaagt
    26161 ttactttgga gactttagcg acagtcagac taatgccgtc aattgtaata gatccttttt
    26221 caactattaa atctaaaatt tctttttgtg tgttgatttg ataccataca gcattatcat
    26281 ctttttttat tgacgagatt tttcctgtac catcaatgtg tcctgtaacg acgtgacccc
    26341 caagtcgacc gttgacagat aaggctcttt ctagattcac ctcacttcca tgttttaata
    26401 gagtaagagc tgttcgactc catgtttcat tcattacatc aactgtaaag gattgatgat
    26461 tgaaatgagt aactgtaaga cagataccat ttactgctat actatcgcct aaatggatat
    26521 ccgttaatat ttttgaggct ttaattgata gtttacaatt acgagagtct ttctgtattc
    26581 tttcaacttt tccgatttct tcaattattc ctgtgaacat ggataaatca cttcactttc
    26641 tatgagatag tcatttccta tttgagaaaa tgcataaggt ttcaatctaa tagcgtcatt
    26701 tggcaaagaa atgccttcac ctccgacagg aaacttggca ctgcctccaa aaatttttgg
    26761 tgcaatatat attttcagct catcaacaat ttgttgttcc aaagcactcc aattcattag
    26821 actgccccct tctagaacta ggctatcaat ctgcatgttt cctagatgtt gcattaaact
    26881 cgataagtct atatgattgc cttttttctt tatggaaagt atttcacagc catgattttg
    26941 atatagcttc attttatttt tgtcttcaga ggaagtggca atgtaagttt taatatcatt
    27001 tgctgttttt acgattttag aggtaagagg agttcgtaaa tgtgtatcgc atatgatacg
    27061 gataggattt ttcccttcct ccaatctaca tgtcagcaaa ggatcgtctt gaataacagt
    27121 attgactccc accataattg cactaacata gtgtcgtaac tgatgcacat gctttcttgc
    27181 ttcttcttca gtaatccatt tggattgatt tgttttagtg gctatttttc catccattga
    27241 cattgcatat ttcataaaaa catagggtac atgctgggta atatactttc taaaactttt
    27301 tattaagtta agacactcat tttctaaaat tccaacagta acttgaagat tattttcctc
    27361 aagtatcttt actccttttc cagatacaat aggattacag tctaggcttc caatgactac
    27421 tcttgtaata ccactatcga ttatagcatc tatacaggga ggtgttttcc cgaagtgaca
    27481 acagggttca agtgttacat aaagcgtcgc tccgacaggg gattctctac agtttttaag
    27541 agcatttctc tcagcatgtg ggccaccaaa aaactcatga taaccttgtc cgatgatgtg
    27601 attatctttt acaataactg cgccgaccat aggattggga ttgacgtaac cagccccttt
    27661 ttgtgccagt tttattgcta atttcatata ttttgaatcg ctcatctcac tacctccaaa
    27721 aaaaatatac cttgaatagg ggactactca aggcatacaa aagaaaactt atgcgattaa
    27781 caaaaatact ctgaaatgac aagtaatcat ttcagagcac gcaaaaagca caaatatact
    27841 tttatcttct ttcatccaga ctatactgtc ggctttggaa tttcaccaaa tcatgccttt
    27901 cggctcgtgg gctataccac cggtagggaa ttacaccctg ccctgaagat agttattcaa
    27961 ttacagatga ttatagtact taattttgaa tatgtcaaca gataaatacc gattgttttt
    28021 gatatactgt atttgtgata atcgattctc gctcctcgga taaagaaaat atgatatact
    28081 agataaacga aataagagag aaggaatact atgtacgcat atttaaaagg aatcattacc
    28141 aaaattactg ccaaatacat tgttcttgaa accaatggta ttggttatat cctgcatgtg
    28201 gccaatcctt atgcctattc aggtcaggtt aatcaggagg ctcagattta tgtgcatcag
    28261 gttgtgcgtg aggacgccca tttgctttat ggatttcgct cagaggatga gaaaaagctc
    28321 tttcttagtc tgatttcggt ctctgggatt ggtcctgtat cagctcttgc tattatcgct
    28381 gctgatgaca atgctggctt ggttcaagcc attgaaacca agaacatcac ctacttgacc
    28441 aagttcccta aaattggcaa gaaaacagcc cagcagatgg tgctggactt ggaaggcaag
    28501 gtagtagttg caggagatgg ccttcctgcc aaggtcgcag tgcaagcaag tgctgaaaac
    28561 caagaattgg aagaagctat ggaagccatg ttggctctgg gctacaaggc aacagagctc
    28621 aagaaaatca agaaattctt tgaaggaacg acagatacag ctgagaacta tatcaagtcg
    28681 gcccttaaaa tgttggtcaa ataggagcag agaatgacaa aacgttgttc gtgggtcaag
    28741 atgaccaacc cgctctacat cgcctatcat gatgaggagt ggggtcagcc cctccatgat
    28801 gaccaagcat tgtttgagtt gttgtgtatg gaaacctatc aggcaggcct gtcttgggaa
    28861 acggtactca acaaacgcca agctttccga gaagcctttc atagctatca aattcactca
    28921 gtcgcagaga tgactgagac tgaattggaa gccatgctgg agaatccagc tatcattcga
    28981 aatagagcca agctttttgc tacacgcgct aacgcccaag cctttctaca gttacaggca
    29041 gagtacggct cttttgatgc ctatctttgg tcttttgttg aggggaaaac tgtcgttaac
    29101 gatgttcctg attatcgcca agccccagct aaaacaccct tatctgagaa attagccaaa
    29161 gatctcaaaa aacgaggctt caagttcaca ggcccagtcg ccgtattgtc ttttctacag
    29221 gctgcagggc tagttgatga ccacgagaat gattgtgagt ggaaaggtct taaatgatgt
    29281 ctaacaaaaa taaggaaatt ctgatttttg cgattctcta tacagtcctc tttatgtttg
    29341 atggcgttaa attgctggct tctttaatgc catctgccat tgcaaattat cttgtttatg
    29401 tagttttagc tttatatggc tccttcttgt tcaaggatag attgatccaa caatggaagg
    29461 agattagaaa gactaaaaga aaattcttct ttggcgtctt aacaggatgg ctctttctca
    29521 ttctgatgac tgttgtcttt gaatttgtat cagagatgtt gaagcagttt gtgagactag
    29581 atggacaagg tctaaatcag tctaatattc aaagtacctt tcaagaacaa ccactactga
    29641 tagctgtttt tgcttgtgtc attggacctc tggtagaaga attatttttc cgtcaggtct
    29701 tattgcatta cttgcaggaa cggttgccag gtttactaag cattattctg gtaggacttg
    29761 tttttgcttt gactcatatg cacagtttgg ctctatcaga gtggattggt gcagttggtt
    29821 acttaggtgg aggccttgcc ttttctatta tttatgtgaa agaaaaagag aatatctact
    29881 atcccctact tgttcacatg ttaagcaaca gcctctcctt aatcatttta gctatcagta
    29941 tagtaaaatg aaataagaac aggacaaatc aatcaggaca gtcaaatcga tttctaacaa
    30001 tgttttagaa gtagaggtgt actattctag tttcaataca ctgtaacatg tgatgaaaat
    30061 gccagtaatg ataccgagaa aaaagctgag aaacttttcc cagctttatt tgttatagtc
    30121 aaagcgaatg acttgttcct gtgcatctac atgagcatgg accccaaagg gtacaattgc
    30181 tcttggagtt gcgtggccga cattcagatt atagacaatc gggatattgc tgtcaatgat
    30241 atctaatagt gcctctttat agtcgtcatg gaaagtttca tccataggtt ttccgaccaa
    30301 gagtccattg atgaccgcga atatgccagt gtcctttaaa gttagcaaca tctttttgaa
    30361 gtcttctggc ttaggctttt cttcgcttgt ttcgagcaag aggattttcc cttcccagtc
    30421 tgacaagtca gggaaaagtt tgtattttgg gcagagttcc gtgctatctg cgtatcgaga
    30481 gttgtcaaag atatcgtaga gggattctag gcaaccaccg aggattttcc cctcgaactg
    30541 agcacttcct tgcaacaagt caaaacctgt atttgtatga ctgacacgag gtgttcccag
    30601 ggccgtggga ctaaaatcag ttcgttcctc ataccaaacg tcactagggc ggatttctga
    30661 aattcttccc gtctcaatca attctttaaa gtagtgaagg ctataggcta gcatttcttt
    30721 gtctaattca caaatgtctg ctaaaaagga ttgaccataa aaagtcttga ttcctaattt
    30781 atgcaacatg aggtggttca tggttgtatc cgagaagcca agaaaaattt tttgcttgat
    30841 aaccttttgg agttggtcat tttcaaaaag ataaggtagc aagcgatagg tatcgtctcc
    30901 accgatggca cataggatca tgtcgatgct atcatcagaa aaggcatgaa tcaaatcctc
    30961 tgcacgagct tcaggatggt ccttgataaa gtctaatcct tttaacgaat ggggcaaaaa
    31021 gatgggattg agtcccagat ccttgagacg ttggataccc aagtccactt cgtgtttgac
    31081 aaaatcctct ccgataatgc cactagataa actaacaata ccaatagtag aaaccatatc
    31141 tcatcctcct agaaatagat tgagcctatt ttatcacaaa atataagaga aagctatgtg
    31201 ggatttcaga agaatacttt taatactctt cgaaaatctc ttcaaaccac gtcagtttta
    31261 tctgcaacct caaagctgtg ctttgagcaa cctgcagcta gcttcctagt ttgctctttg
    31321 attttcattg aatataactc aaaaaagtaa gtgaaaaagc aaccgcacct gcagttgctt
    31381 ttatcgtttt cttattcttg atagcgtgtt ggtcctgcac ttgcactacg gatattcaag
    31441 cgtttcttga gatagaagcg aagggctagg agggctgctc caataatagc tagtggcaat
    31501 ggatccagta ctgggttaag gctagctggt aggaagcttg ttgcaaagaa gacaagcaac
    31561 caaaggaaca tagaagctag gataactagt acagatttcc agaaaggtgg acgttgactg
    31621 cgatccatat ctggtccata gtattggtaa acaaagtagt acatcaagta gaaggcaaat
    31681 ccaccaacca gtccaactaa tagaagagta atcaatccat agccgaaagc ttggtctgcc
    31741 gcaaagaagg ttgtgagggc gctgacaagg gcaaagaggc tagtgatgaa aagagctgag
    31801 tccataatca tcagttttgg gtcatcattt tcttttggat gctctttttc gtactgctct
    31861 ttgacagtga agctatgagc ccaatgagtt ggtgcgccgt atagggaacg ggcagttgta
    31921 cctttagatt gctcctcaag gatttgggga atgacttcct caaaaatagt ctggatttca
    31981 gcgtctgttt tcccatcttt gatgaattgt tgggtagcaa tgtggacaaa ctcttggttt
    32041 ttcttagtta atttttgtag atcaatttga gacataggaa ctcctcttag aaccattttt
    32101 tatggatgag atagagagtg agcgagacac tcatagcaaa ggcgataaag acgattaacc
    32161 agaaggcatt tggctctccg tttaggggga tttcattatc cttaaagttc atcccgtagg
    32221 cagaaaagac catggttggg atggacatga cgatggtcac aagggccaag gttttcatga
    32281 tgttgttctg gttgttagaa atgatagagg caaaggtctc tgtcatagaa tgcaagacgt
    32341 ttccataaat atctgccatc tcgatggcct gttgggtttc aatcagggtg tcttcaagca
    32401 ggtcttcgtc ctcaaggtat ttcttgatat tgctggttga actggtcaat ttcttaatca
    32461 cgcgctcatt tgttttgagg gaggccttga aatagacgat agttttttcc aattccatga
    32521 gctcaatcaa ttcttcatta cgagttgatt gatgcagttg actttcgatt tgttcactct
    32581 tgcggtcgat tgaacgaagg gctgttaggt aaagctctgc attgcgataa agaatttgaa
    32641 agataaaacg tgaacgcatg aaggtataga aattacgcaa tcgacggttg ataaagacat
    32701 caaggacagg tagtggttcc aaacacgtag tgataatggt ttcctcagtg atgataatac
    32761 caagcgggat ggttacgtag taggtgcggt tatttctttc ctccgtgacc ggcacgtcta
    32821 cgataatcag ggtatactcg tcttcaatgg taatacgaga catttcttcc gcatcgagcg
    32881 gtgctcgaag gtcggcaata tcaatatcga aggtgttggc gatttcgagt gattcatttt
    32941 gagtcggatt gacgagattg atccaagtac ccggttcaag cgtatcgatc tctttaaatt
    33001 cagttgttgt agagagaaaa acttgtttca tatcccttat cctttctcat ttttcagatt
    33061 ttttcacact gtactattat actacaaaat cggccttttt caaaataaat ctaacaaaaa
    33121 acctgttgct attggatttt tgctttgtct tgaaatgttg atttttaaat ttgctcccct
    33181 ttttgctccc cctatagtgc ttttagtgca gtttcgtaga atgatactgc tttttttgcg
    33241 ttctcttttg agaggtggct gtatatatcc atggtcattg ataaagtgga atgacctagg
    33301 cggtactgga gttccttgta tggtattcca gagttaagca agagactagc gtgggtgtga
    33361 cggaatccat gaaagccaat gttaggaaca tcagcacgct taaaatgtgt ttgcaatctt
    33421 cttttaagtc ttgaactgct aggatactca tgaatgaaat cagaaaatac tacactttca
    33481 cgttgcccta tcttccaagc ctctttagtt tggcgtagtt tgtactgctt cagcatgcta
    33541 acagtcgctt gatctatatc tatatcccga taactagcct tagattttgg gctattaatt
    33601 tctaaatcac gatttagtgt tttggtaaca tgtaccacgg cattatctaa atctatatca
    33661 gaccaggaaa gagctaaagc ttcattgata cggcaaccag tagccaatag gaacttatag
    33721 agcgttgtct catagtagta acgatatcta tcgctatcta aattatcgag ataagcaaga
    33781 aactttttta gttcttgatt ttcaaagtgt tttacttttt tccgctttgc tttctgagtg
    33841 ttacgaggta aaacaacatc acgggcagga ttggacggta tagcctgcat agttacgcca
    33901 tactgtaaga tacgtttgtt tagagcgtgt atcttgtcat aatgtagaaa agcccctttt
    33961 tcccctctat tagtcttgtc agcaagttcg ttgataattg actggataag tggagtagtg
    34021 agtttatcga gcttataagc cccaaaaata ggcaatatat gattatcaat taactttcta
    34081 acattgtctt gagtgtttgg cttaaccgta ttcttataac tttctaacca gagattggct
    34141 aattcttgat agttttttat ggtgctagcc ttttgtctag tagatcctgc tttttgaaaa
    34201 gctatttttt cttgagtagc tttctgctta acttcctttt gtgtccgtcc cgttacctta
    34261 gttttaactt tctttcctgt aacctggtca actcctagat atatattagc acgatagact
    34321 ttactaccgt ctttttttat aacttcagtt attttcatga tttaaacctt tctaaaatac
    34381 atcagcaggc aagccattat caaaaaggtt ttatatcatg ctagggcttc acagattgcc
    34441 ctgtattcaa gtttaacaag ttagataggt aaattatatg cttaattgtt aagtggtaaa
    34501 aacccttatt ttacagtgtt ttttgagagt ttttttctaa agttagttta ccagttagct
    34561 aacaaaacac tttacatttc ccacgcgcgt ggtataatag aattaacaag ataacttgcg
    34621 aaggattaac gttgtgttcc caaaggggag taagtcaatg gacgagaatt cacatatgcc
    34681 tggggttatc ttattttttt tgtctttttt tcaagttttc tacgaacgtt tcaggatttc
    34741 gttcgatttc ctcaattata aaatcgcaga ggatttgaga gtagccgtat cgattaccaa
    34801 ttttataatg gtagcaataa cgttgatttg tcttgatatc ataaaatgta ttaaaaagtt
    34861 gtaagtcgtt tgttgtaaac gtcttttttt gtttttctcc accgactatt tttgttaata
    34921 gaatcccttt cgatttgaga tatctattaa caattttaat aacttcccta gtagttaatg
    34981 gatagatagc gtttggatct ttaatttctc taactatacc tatactcgtt tccgcagtgt
    35041 tatcaagttt aacaaataaa tcagcatctt gttttcgctt agtgatataa aatcgagttt
    35101 caacaggtac agcatagtca gaattattag tagatatttc actttcaatt ttatcttgct
    35161 cggcgagtat acgttcagcc attttaggag agtacttggc tcgaatttct tcttgattaa
    35221 gttggtcgat gcggactgat aaagtcaaga aattttgtgc aatttgtttt gtgatatcct
    35281 cattgtgaaa ctcttgcatt tttaaaatat aattagaaac acatgcttga aataatgggg
    35341 catagatatg ttcataatct tctgttatga agtgggtact agtattccta agttctataa
    35401 ttcgaattag attttttcgt aatgatccat gtttatctgg aaatactgtt tcaactgcat
    35461 tttctaatga tatcgttcta tccttactat ccttaaaata tattgattct tcaccatcat
    35521 tattaatgat ataagccttt agcattaatt cccaggcgtt acagataaaa aaactaaaac
    35581 cctcaattct atacttaatt gtaggtttgt tatatatttc aatccccata ataaaagcct
    35641 caatactttt attaactaat cttttactta aatcttccat acgtttcctc tcttaaaata
    35701 ttttctatat tgtggcgcgt ggtatgctat aggtcgcatt ttcggcactt tcggccatcc
    35761 tgttttgcta tggttgcttt tttttattaa gtaattcttc aactttaata atatcttctt
    35821 ctgaatgact atttctaaaa ttattaatag cttcagtatc tttagggtct attaaggcgt
    35881 attcattaga aatttttaaa attctaattt cttcctcaag catagcattt tctttttcaa
    35941 gcatttttag ctctttatca attctcagtt tttcatctaa ctgtgaacct aaatgagtaa
    36001 cgaggtcaga gggttctttt aaaaaatcat gaggtaaatt atcatgctct aaatctttta
    36061 taacgccttc aaaaaatttc ttttgttttc tttctgataa agattcaaac attgtaacaa
    36121 aatcaatgac aggttgctca tattttaaaa taacgtctgg gtcaataaca aaataacggg
    36181 gatcaatatc tgatgcttcc actttaaaat agtcagcaag tttttggagg ttttcggtgt
    36241 ttggcattga cctacctttg acataacctg ttaaggtact ttttggaata cctaaatcat
    36301 tatgtaagtc aatttgtctt ttaccttgtt ctttcataag tctatttaac ctagcagaga
    36361 aatagagtct attttttata tcttgagggc tattttttgc catgtataat tacctcctcc
    36421 taaatttcct aagcccattg taacacaata aaatagaaat agtacga
//
LOCUS       DAVKQL010000015        35286 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVKQL010000015 DAVKQL010000000
VERSION     DAVKQL010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35286
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.015"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            <1..221
                     /locus_tag="VW561_000672"
     CDS             <1..221
                     /locus_tag="VW561_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942965.1"
                     /translation="KKSNMSLVFLFTFLFVESLFWIYQLDNGIIGLLPIFQYMVNSNP
                     YALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            231..929
                     /locus_tag="VW561_000673"
     CDS             231..929
                     /locus_tag="VW561_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9942966.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
     gene            926..1573
                     /locus_tag="VW561_000674"
     CDS             926..1573
                     /locus_tag="VW561_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9942967.1"
                     /translation="MNEIITLKNIQLELKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(1660..2526)
                     /locus_tag="VW561_000675"
     CDS             complement(1660..2526)
                     /locus_tag="VW561_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9942968.1"
                     /translation="MKLFKPLLTVLALAFALIFITACSSGGNAGPSSGKTTAKARTID
                     ENKKSGELRIAVFGDKKPFGYVDNDGSYQGYDIELGNQLAQDLGVKVKYISVDAANRA
                     EYLISNKVDITLANFTVTDERKKQVDFALPYMKVSLGVVSPKTGLITDVKQLEGKTLI
                     VTKGTTAETYFEKNHPEIKLQKYDQYSDSYQALLDGRGDAFSTDNTEVLAWALENKGF
                     EVGITSLGDPDTIAAAVQKGNQELLDFINKDIEKLGKENFFHKAYEKTLHPTYGDAAK
                     ADDLVVEGGKVD"
     gene            complement(2538..3295)
                     /locus_tag="VW561_000676"
                     /pseudo
     CDS             complement(2538..3295)
                     /locus_tag="VW561_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            complement(3296..3973)
                     /locus_tag="VW561_000677"
     CDS             complement(3296..3973)
                     /locus_tag="VW561_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9942969.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIEISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLDFTRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GTAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTH
                     LEKHWRN"
     gene            complement(3933..4553)
                     /locus_tag="VW561_000678"
     CDS             complement(3933..4553)
                     /locus_tag="VW561_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9942970.1"
                     /translation="MYIAVWGILGSFLLGLIVSIIRHYRIPVLAQVATAYIELSRNTP
                     LLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMAESFRSGLEAISQTQQEIGLA
                     IGLTPLQVFRYVVLPQATAVALPSFSANVIFLIKETSVFSAVALADLMYVAKDLIGLY
                     YETDIALAMLVVAYLIMLLPISLVFSWIERRLRHAGFGNPSTLSGK"
     gene            4586..4732
                     /locus_tag="VW561_000679"
     CDS             4586..4732
                     /locus_tag="VW561_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HEU9942971.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TSFQ"
     gene            4860..6350
                     /gene="lysS"
                     /locus_tag="VW561_000680"
     CDS             4860..6350
                     /gene="lysS"
                     /locus_tag="VW561_000680"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEU9942972.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            6974..8110
                     /gene="lctO"
                     /locus_tag="VW561_000681"
     CDS             6974..8110
                     /gene="lctO"
                     /locus_tag="VW561_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEU9942973.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     regulatory      8364..8452
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8667..9335
                     /locus_tag="VW561_000682"
     CDS             8667..9335
                     /locus_tag="VW561_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEU9942974.1"
                     /translation="METQDYAFQPGLTVEELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     gene            9578..10360
                     /gene="thiM"
                     /locus_tag="VW561_000683"
     CDS             9578..10360
                     /gene="thiM"
                     /locus_tag="VW561_000683"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU9942975.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALSVSPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKKFKRVK"
     gene            10362..10991
                     /locus_tag="VW561_000684"
     CDS             10362..10991
                     /locus_tag="VW561_000684"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU9942976.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFDTQSKYDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
     regulatory      11200..11288
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(11505..14681)
                     /gene="carB"
                     /locus_tag="VW561_000685"
     CDS             complement(11505..14681)
                     /gene="carB"
                     /locus_tag="VW561_000685"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU9942977.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKEGPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDTLIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDKVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
     gene            complement(14994..16073)
                     /gene="carA"
                     /locus_tag="VW561_000686"
     CDS             complement(14994..16073)
                     /gene="carA"
                     /locus_tag="VW561_000686"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEU9942978.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            complement(16123..17046)
                     /locus_tag="VW561_000687"
     CDS             complement(16123..17046)
                     /locus_tag="VW561_000687"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEU9942979.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGSELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            complement(17065..17586)
                     /gene="pyrR"
                     /locus_tag="VW561_000688"
     CDS             complement(17065..17586)
                     /gene="pyrR"
                     /locus_tag="VW561_000688"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEU9942980.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            complement(17797..18426)
                     /gene="nth"
                     /locus_tag="VW561_000689"
     CDS             complement(17797..18426)
                     /gene="nth"
                     /locus_tag="VW561_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU9942981.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            complement(18426..18968)
                     /locus_tag="VW561_000690"
     CDS             complement(18426..18968)
                     /locus_tag="VW561_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEU9942982.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNHLNQEILDDD
                     LVLPIENEALDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            19138..19455
                     /locus_tag="VW561_000691"
     CDS             19138..19455
                     /locus_tag="VW561_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEU9942983.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EKLNNSILIDLEKFYQPTSLLIGLGSLKLNDQARTAWRNYDKFHYEHIKHVLSLYGPV
                     FGF"
     gene            19694..21253
                     /locus_tag="VW561_000692"
     CDS             19694..21253
                     /locus_tag="VW561_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9942984.1"
                     /translation="MHYEHSRKGNHMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKILMGEALSDFTIKGNIQSDYQSLAYIPQKLPEELKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQKIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRTYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLKQLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYISKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEICSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(21402..22301)
                     /gene="htpX"
                     /locus_tag="VW561_000693"
     CDS             complement(21402..22301)
                     /gene="htpX"
                     /locus_tag="VW561_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEU9942985.1"
                     /translation="MLFVQIASNKRKTWILLVVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALVSAITMLSGMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(22303..22863)
                     /locus_tag="VW561_000694"
     CDS             complement(22303..22863)
                     /locus_tag="VW561_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU9942986.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNIKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            22957..23670
                     /gene="rsmG"
                     /locus_tag="VW561_000695"
     CDS             22957..23670
                     /gene="rsmG"
                     /locus_tag="VW561_000695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEU9942987.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            24022..25305
                     /locus_tag="VW561_000696"
     CDS             24022..25305
                     /locus_tag="VW561_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEU9942988.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLEANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLIGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(25500..27071)
                     /gene="ffh"
                     /locus_tag="VW561_000697"
     CDS             complement(25500..27071)
                     /gene="ffh"
                     /locus_tag="VW561_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEU9942989.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPNRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(27083..27415)
                     /locus_tag="VW561_000698"
     CDS             complement(27083..27415)
                     /locus_tag="VW561_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEU9942990.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            complement(27506..27883)
                     /locus_tag="VW561_000699"
     CDS             complement(27506..27883)
                     /locus_tag="VW561_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEU9942991.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            27955..29259
                     /locus_tag="VW561_000700"
     CDS             27955..29259
                     /locus_tag="VW561_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU9942992.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSQRFVNRKVFKSITFSQEDQDQLASMRKLAE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            29272..30078
                     /gene="yidA"
                     /locus_tag="VW561_000701"
     CDS             29272..30078
                     /gene="yidA"
                     /locus_tag="VW561_000701"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEU9942993.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYERYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            complement(30133..30204)
                     /locus_tag="VW561_000702"
     tRNA            complement(30133..30204)
                     /locus_tag="VW561_000702"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30169..30171),aa:Arg,seq:cct)
     gene            complement(30246..30593)
                     /gene="rplS"
                     /locus_tag="VW561_000703"
     CDS             complement(30246..30593)
                     /gene="rplS"
                     /locus_tag="VW561_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU9942994.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(30712..31041)
                     /gene="crcB"
                     /locus_tag="VW561_000704"
     CDS             complement(30712..31041)
                     /gene="crcB"
                     /locus_tag="VW561_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU9942995.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(31035..31409)
                     /gene="crcB"
                     /locus_tag="VW561_000705"
     CDS             complement(31035..31409)
                     /gene="crcB"
                     /locus_tag="VW561_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU9942996.1"
                     /translation="MKKEQFYPLGIFIAAMLGGLIRYLVSIWLPASPDFPWGTLLVNY
                     LGIFCLIYLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDAVKLLDTGRYLSLI
                     LYLLLSIGGGLLLAYYLGRKKW"
     gene            complement(31406..31672)
                     /locus_tag="VW561_000706"
     CDS             complement(31406..31672)
                     /locus_tag="VW561_000706"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEU9942997.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEEVVAYKKASGKPILD
                     TKRETVIFEKIRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            complement(31788..32231)
                     /locus_tag="VW561_000707"
     CDS             complement(31788..32231)
                     /locus_tag="VW561_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEU9942998.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRIFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEDLAAKVG"
     gene            32335..33270
                     /locus_tag="VW561_000708"
     CDS             32335..33270
                     /locus_tag="VW561_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEU9942999.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQEVLKQYNVTDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            33324..33608
                     /locus_tag="VW561_000709"
     CDS             33324..33608
                     /locus_tag="VW561_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEU9943000.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(33665..34243)
                     /locus_tag="VW561_000710"
     CDS             complement(33665..34243)
                     /locus_tag="VW561_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron permease"
                     /protein_id="HEU9943001.1"
                     /translation="MVRNYWKAKSWLLVLALSFLILSPAGAQESLSFFFVKITDASKA
                     VKNGGQTETQKLVTKMASDFERVENKDSEVGKIVKEKLALSGDITEAKLTEISSALLA
                     FEKEQNPVDLDAEKEKLVNRLSPRFETLEQAIASKDLEKVREAFKKMNSTWTINESMV
                     RDNSTAHYGRVETAISFLPSSMEIEPTDESGT"
     gene            complement(34218..35229)
                     /locus_tag="VW561_000711"
                     /pseudo
     CDS             complement(34218..35229)
                     /locus_tag="VW561_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259617.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
BASE COUNT        10574 a         7322 c         6999 g        10391 t
ORIGIN      
        1 ttaaaaaatc aaacatgagc ttagtatttc tgtttacttt tttatttgta gaatccttat
       61 tctggattta tcagttggac aatgggataa ttggattatt gccaattttt cagtatatgg
      121 taaattccaa tccgtatgca ttgatttatt ggcttacatt actatctatc ataattccat
      181 tgactgtatt ttctgttcat agaaactgga ggagagtgta aaagttggaa atgggaaagt
      241 taagtagtca catgtggagg ttgaatcaga taatctatac caagtacttt tggggttatg
      301 ttcttttttg gatattgatt tgtttaggat tatggtattg gttagaagga aatgatagac
      361 ttgttataga aattttaaaa gggcctaatc tgagtcaaaa ctctttttta gtcttatcca
      421 tatggttgct tcattggttt attattcata cattttttct agcagttgta tatcgtagaa
      481 gagcatccga tttctttatg gaagtgattc gattttcttc tattaagctc tggattaggt
      541 atcagatttg gacctgtttt ctttatggac tcattttaat catggtaaaa gttctagtga
      601 ttcaatttat gttacagtta ccaaactggg atataggagt tttgtttata gttgattctt
      661 tgaatgcttg tgtgttagtc ttgttttgct ttatgttata cgcactagga gcgaatgtac
      721 aaatgaactt tgcttgcgtt agtttctttt tactcatgat tgtgtttggt ggtctatttg
      781 taggaaatcg aactaattat ctattttata ttttgaatcg agggaatgga gatataggac
      841 gagatttatt tcttcagttg ctatttttag tctttctttt taaaagcatt ttctatttca
      901 ctcgtcaaaa gaggaggttt atagaatgaa tgaaataatt acattaaaaa atattcagtt
      961 ggaattaaaa aaaacatgtg tttttcaaaa ccttaatttt agttgtaaac agggggaaat
     1021 tataggaatt actggtgcga atggctcagg gaaaagtgta ttgtttaaat taatagctgg
     1081 tttatatagt ccgtcttatg gagaagtgtt aatcaatggg gaaaatattg ttcctgagag
     1141 aaaaattcca gctaatttgg gagctttgat tgaagaacct ggttttataa attattatag
     1201 tggctttaag aatttacaat atttggcaag catacgagga gtagttggta atcaggaaat
     1261 caatgataca ctgaaaatag ttggtctata tgagcaaaaa gaccagaaag ttaaaactta
     1321 ttcgctaggt atgaggaaaa agctagggat tgctcaagca attatggaga atccctctat
     1381 tcttttacta gatgaaccta tgaatgcctt ggataaatca agtgtagaaa atatgagaac
     1441 attgtttaga aagctctcta gtgaaaaagg gacaacaatt ttgattgcta gtcatagtga
     1501 agaggatatt cgtatcttat gtgataaagt atatgcaata gaagataaag tatgtacact
     1561 gtgttcagat tgaacatctt agatagactc aggatgttat aggtgaaatc ttaaaaaggg
     1621 attggatctt aaaatccagt tccttttaag agttaatgac taatcaactt ttccaccttc
     1681 aacaaccagg tcatctgctt tagcagcgtc accgtaggtt gggtgaagtg ttttttcata
     1741 ggccttgtgg aagaagtttt ccttgcctaa tttttcaata tctttattga tgaagtctag
     1801 caattcttgg ttgccttttt gaactgctgc cgcaatggta tcgggatcac cgagggaagt
     1861 aattcctact tcaaatcctt tattttcaag cgcccaagct agaacttccg tattgtcagt
     1921 tgaaaaggca tctccacgtc cgtcaagaag agcttggtaa gagtcactgt attggtcgta
     1981 tttttggagt ttgatttctg gatgattctt ttcaaaataa gtctcagcag tcgttccttt
     2041 tgtgacaatt aaggttttac cttcaagttg tttgacgtct gtaatgagac cagtcttagg
     2101 tgatacgaca cccagagaaa ctttcatata tggaagggca aaatcaactt gtttcttacg
     2161 ttcgtcagtt actgtaaagt tagcaagagt aatatctacc ttgtttgaaa tcaagtattc
     2221 cgcacggttg gcagcatcga ctgaaatgta tttaaccttg acaccaaggt cttgagctag
     2281 ttggttccct agttcaatat cgtagccttg gtaagaacca tcattgtcaa cgtagccaaa
     2341 cggtttttta tctccaaaca cggcgattcg cagttcaccg ctttttttgt tttcatcgat
     2401 agtgcgagct ttggcagttg tttttccaga ggatggacca gcgtttccac ctgagctaca
     2461 agcagtgata aagataaggg caaaggcaag tgctaaaaca gttaagagtg gtttgaatag
     2521 tttcataaga atctccttta tagatatgag ccgaattggc taaagtcaaa gacgtttaaa
     2581 aattcctggg ctcgtttggt ttgcggattg gtaaagaagg cttgagctgt tccttcttca
     2641 gcgattttcc cttggtcgag gaagataatc cggtcagcaa tggcttgggc aaactgcatt
     2701 tcgtgggtta ctaaaatcat ggtacggcct tcttggacca aatcattgat aagttccagc
     2761 acctcacgca ccatttctgg atccagcgaa gcagtcacct cgtcaaaaag gatgatttct
     2821 ggatgcatta ggagggcacg gacaattgca acacgttgct tctgtccacc agataattga
     2881 cgggcaaagc tatgttgttt atccagcaaa ccgacacgct ctagtaattg caaagcttct
     2941 tccgttactt ctttcttgtc ccttccttga gctttgatag ggcctaggat gaggttttgt
     3001 aagacatcca gatggggaaa gagttcataa ctttgaaaga ccatgccaat cttttggcga
     3061 actaggtgaa aatctttttt attttcaacg atagactgac catccagaag aatatctcca
     3121 ccttgaatac tttctaagcc gttgaggcaa cgaaggaggg tacttttccc acaaccagat
     3181 ggccctagga tgacaacaac ttcccttttt tgatttctag agaaagtcct tggaggatgg
     3241 gattgtctcc gaaggatttt tttagttcct tgatttctaa gatagtttca gacatttagt
     3301 ttctccaatg tttttctaag tgagtggata gtttggaaat agggtagcaa actgcgaaat
     3361 ataagactag aatggttcca taaatccaaa atgaagcagt tgggatggtc aggcgattgc
     3421 tatcgatgat ttgttgtcca actttggtca cttccacaac cccaatcaaa acaactaatg
     3481 aagtggtttt aatcatccga gtgacaagat tgatagcctg cggtagcagt cttcttaaga
     3541 cttgtgggat gatgatgtgg tagtaaagtt gaacattagt caagccgagt gcctgtccac
     3601 tttcaaactg atgtttaggg agagaagtga tagctccacg taccaagtct cccatttcag
     3661 ctgttcccca gagggtaaaa acgataatag ctgaagtctc acctgagata ttgatattaa
     3721 agtttcgagc taagccaaag taaacgatga agagtagcac cagctggggc atgatacggg
     3781 taaagtccag atacaatcgt gttaaaaatc gtatgattct agaatgggag gtcatgatga
     3841 ttcccatgac tgttccgaac atcatggata agaggacaga caggatggat atctcaatcg
     3901 taacgcccaa tccctgtaag attctcagga gattatttcc ctgaaagagt acttggattc
     3961 ccgaatcctg catggcggag cctcctttct atccagctaa agaccagtga gatgggtagc
     4021 agcatgatca gataagcaac taccaacata gctagcgcaa tgtctgtctc atagtagaga
     4081 ccaatcaaat ccttggcgac atacatgagg tcggccaaag ccactgctga gaaaacagag
     4141 gtttccttga taaggaaaat gacattggca ctaaaggagg ggagtgccac cgctgttgct
     4201 tgcggaagaa ccacatagcg aaagacctgt agaggtgtca gaccaatagc gaggccaatc
     4261 tcctgctggg tttgactgat ggcttccagc ccacttcgga aagattctgc catataggat
     4321 cctcctaaaa agacaagccc aagcgttgca cagacttctg aagataggac aatcccgatt
     4381 cggggaagac cgaagtagag aaagaagagt tgaatcaaaa ggggcgtatt acgtgacaat
     4441 tcaatgtagg ctgttgctac ttgcgccaaa acagggattc gataatgtcg gatgatacta
     4501 acgattaaac cgagcagaaa ggatcccaaa attccccaaa ctgcaatata caaagtaaac
     4561 gcttacttat caaggagggc attttatgtc atacaaaaca agcaatgcag aaggtcatgt
     4621 agatttcatc aatacctatg atttggagcc aatggcgcaa caagttattc ctaaagcagc
     4681 atttggctat atcgctagtg gggcggaaga tactttcact tctttccagt gattttagcg
     4741 tcaggttctt tttagttttt aaagattatc cgtgaatttc ttgcttattt atgataaaat
     4801 gggggtatcg caaaaaatga ctcatcgtat tcaattttga gtaaaactag gaggatccca
     4861 tgtctacaga acatatggaa gaactaaatg accagcagat cgttcgccgt gaaaaaatgg
     4921 ctgcgctccg cgaacaagga atcgatcctt tcggaaaacg ttttgaacgt actgcaaatt
     4981 cacaagaatt aaaagataaa tatgccaacc tcgataaaga acaattacac gataaaaacg
     5041 aaacagctac tatcgcagga cgcttggtaa ccaaacgtgg taaaggaaaa gttggttttg
     5101 cccaccttca agaccgcgaa ggccagattc agatctacgt tcgtaaggat gctgtcggtg
     5161 aagaaaacta cgaaatcttc aaaaaagcag accttggtga cttccttggt gtcgaaggtg
     5221 aagtgatgcg tacggatatg ggagaactct ctatcaaggc aacccacatc acacacttgt
     5281 ctaaggctct tcgtcctctt cctgagaaat tccatggttt gacagacgtt gaaacaattt
     5341 accgtaaacg ttaccttgac ttgatttcta atcgtgaaag ctttgaacgc tttgtcactc
     5401 gttcaaaaat catctctgaa atccgtcgtt accttgacca aaaaggattc cttgaagtgg
     5461 aaacacctgt tcttcataat gaagccggtg gtgctgctgc ccgtccattt atcacccacc
     5521 acaatgccca aaacattgac atggtgcttc gtatcgcgac tgagcttcac ttaaaacgcc
     5581 ttatcgtggg tggtatggaa cgtgtctatg aaattggccg tatcttccgt aacgaaggaa
     5641 tggacgctac tcataaccct gagttcactt ctatcgaagt ttaccaagct tatgcagact
     5701 tccaagacat catggacttg actgaaggca ttatccaaca cgctgctaaa tcagtcaaag
     5761 gtgatggccc agtcaactac caaggtactg aaatcaagat taacgaacca tttaagcgtg
     5821 ttcatatggt ggatgctatc agagaaatta ctggtgttga tttctggcaa gacatgactt
     5881 tggaagaagc taaagctatc gctgctgaga agaaagttcc agttgagaaa cactacactg
     5941 aggttggtca catcatcaat gccttctttg aagagtttgt tgaagaaact ttaatccaac
     6001 caacctttgt ctatggacat ccagtagctg tatctccact cgctaagaaa aatcctgaag
     6061 accaacgctt tactgaccgt ttcgagctct ttatcatgac taaggagtac ggtaatgcct
     6121 ttactgagtt gaacgaccca atcgaccaac ttagccgttt tgaagcccaa gctaaagcta
     6181 aagaacttgg tgatgatgaa gcgacaggca tcgactatga ctacatcgag gctcttgaat
     6241 acggtatgcc accaacaggt ggtttgggaa tcggtatcga ccgtctctgc atgctcctca
     6301 ctgatacaac aactatccgt gatgtattgc tcttcccaac aatgaaataa attcttaccc
     6361 tctggctctt accaggggat tttttgattc aaaaagagac tgaatttaag gagaaaatga
     6421 agtgtagtat attgaagttg aaatagtaca ctttgatttc taagacactg ttagaaattg
     6481 gtttaaattc cctaatcaat ttgtgcatgt tttatttcat tttacgatag tacgctgaaa
     6541 cttttcaaaa agtactagaa attgacttgg attccccaat tgatttgttc agattcacta
     6601 taaataaaaa attaataagt gggataggaa gttagcgtca actaggatag tatcttgctt
     6661 aaacagtata tatgggactg atataagtcc ataggtccta ttagaggatg ttctggtgtc
     6721 ttattcactt gttttttata gtattagtag atagaatcag caaataaaaa agtagttcaa
     6781 atgcctggca gcaaaaaatc tcaacgattc acttaatata gactaaggtt tagagtttaa
     6841 ggaaagtagg gctagtatat tgtatgaaaa aataagatgg tttttaagca aaactcttga
     6901 aaatgattga aaaaagagtt attatagttg tatagtaaaa agcaaacgct tacttaatcg
     6961 aggaggacag tttatgtcat acaaaacaag caatgcagaa ggtcatgtag acttcatcaa
     7021 tacctatgat ttggagccaa tggcgcaaca agttattcct aaagcagcat ttggctatat
     7081 cgctagtggg gcggaagata ctttcacttt aagagagaat atccgcgcct ttaaccacaa
     7141 actcatcgtt cctcatacac tttgcgatgt tgaaaatcca agtacagaga ttgaatttgc
     7201 aggtgaaaaa ctatcttcgc caatcattat ggcacctgtt gcggctcata aattggcaaa
     7261 tgaacagggg gaagtggcga ctgcgcgtgg tgtgcatgag tttggttctc tttatacaac
     7321 cagttcttac tctactgttg accttccaga gatttctgaa gcccttcaag ggacacctca
     7381 ttggtttcaa ttttacttta gtaaggatga cggtatcaac cgccacatca tggaccgtgt
     7441 gaaggctgaa ggttataaag cgattgtctt gacggcagat gctactgtag gggggaatcg
     7501 tgaagtggat aagcgtaatg gttttgtctt cccagttggc atgccgattg ttgaagaata
     7561 cctgccagaa ggtgctggta aatcaatgga ctttgtttac aaatcagcta aacaacgctt
     7621 gtctccacgc gatgtagaat ttatcgctga atactctggt cttcctgtgt atgtcaaggg
     7681 accacaatgc cgtgaggacg ttgaacgttc gcttgctgca ggagcttctg gtatctgggt
     7741 aaccaaccac ggtggtcgtc aaatcgacgg tggaccagct gcctttgact cgcttcaaga
     7801 agtggcagaa gcagttgata gacgtgtgcc gattgtcttt gactctggtg ttcgtcgtgg
     7861 tcaacacgtc tttaaagcct tggcttcagg agcagacttg gtagctattg gtcgcccggt
     7921 tatttatggc ttggctctcg gtggtagtgt cggtgtgcgt caagtctttg aacacttgaa
     7981 tgcggaattg aagacagtta tgcaattgtc tggagctcag accattgaag atgtcaaaca
     8041 ctttaagctc cgtcacaatc catacaaccc aaccttccca gttgaccctc gtgacttaaa
     8101 attgtattga taaaacagat tgcctccact gaatgtggag gtttttcttc gtgtattgga
     8161 agaataaatc gctatcgaaa accatagtaa aatggaataa aaacaggaca aatcaatcag
     8221 gatatttaaa tcgatttcta acaatgtttt agaagtagag gggtactatt ctagtttcaa
     8281 tctactatat ataaagatat ttttcggtta aatatcttgc taaggctttc attttttgct
     8341 atactggttc agtaattaaa taatagagac atttggggtg ctttaagctg agatgatacc
     8401 cattgaacct gatacagtta agactggcga agggaaatgt gaaacgtttt ttcgtatggc
     8461 gaaatgaacg atctaatctt gtaagtcaac tctaattctt gattgtaatt ggagtttttt
     8521 gtttatataa actggatagg cttgtttagt tagctctctg caaatgctct cgatgtgctt
     8581 taaaaggatc aagtggaatt acgattttgc ttgatatttc ttggtgtttt attaattact
     8641 aacaagtaaa gagaggagaa gtaaatatgg aaacacaaga ctatgcattt cagccaggtt
     8701 tgactgttga agaattatta aaaagcagtc agaaggattg gcaggctgca atcaatcatc
     8761 gttttgttaa ggaacttttt gcggggacaa ttgagaataa ggtcttaaag gactatctga
     8821 ttcaagatta tcacttcttt gatgccttct tatccatgct gggtgcctgc gtagctcacg
     8881 cagataaact tgaatccaag cttcgttttg ccaagcaact aggctttctt gaagcagatg
     8941 aagatggtta tttccaaaag gcttttaaag agttaaaagt agcagagaat gactacctag
     9001 aagtgacttt gcaccctgta acaaaagcct ttcaggatct aatgtattcg gcggtggctt
     9061 catcagacta tgcccatctt ttggttatgc tggtcattgc agaaggtctc tatttagact
     9121 ggggttctaa agatttggct ctacctgaag tctatattca ttcggaatgg atcaatctcc
     9181 acagaggtcc tttctttgca gagtgggttc aatttctggt tgacgaactc aatcgtgtcg
     9241 gtaaaaatcg agaagatttg acagaacttc agcaacgctg gaatcaagca gtcgctttag
     9301 aattagcttt ttttgatatt ggttacgatg tctagagaaa gctcatgata tttacaaatt
     9361 tgatgaggta agactcttcg aaaatctctt caaaccacgt cagttctatc cacaacctca
     9421 aaacagtgtt ttgagcaacc tgcggttagc ctcctagttt gctctttgat tttcattgag
     9481 tataaatcat tcaatgtaaa tgaaaaaaat tcttaacgaa agatagaaat aaatcgaaat
     9541 cagtagatcg tataaagtga aaaggaagga tttcaatatg acaagtttaa aattattaaa
     9601 agaaaaagca ccattggtca tttgtataac caatgatgta gtaaaaaatt tcacagcaaa
     9661 tggattagta gcactgagcg tctcaccagc catgagtgag tttccagcag atttagagga
     9721 tttgttaaag tatgctggtg gtttattaat aaacatagga acattgacag atgaaaattg
     9781 gaaattatac caagctgctc tgaaaattgc ggagaaatat aatgtcccag cagttttaga
     9841 tcctgtagcc tgtggagcag gagaatatag aaaaaaagta gcagatgatc taatcaacaa
     9901 ttataaacta gcagcgatta gaggaaatgc tggcgagatt gcctctttag taggaataga
     9961 tgtggcatct aaaggagtag atagtgcggg cgtagataat attgacgaaa ttgctctagc
    10021 agcaaatgag aagttcaata ttccaatagt agtaacaggt gaagtggatg ccattgctgt
    10081 taatggagaa gtggtaacga ttcataatgg tagtgctatg atgccgaaag tcattgggac
    10141 aggatgctta ttaggagctg tagtagcaag ttttatcgga ctggaaaaag gtcaagaatt
    10201 aaaatcatta gaaacagcaa tgttagttta caatatcgct ggagaaatgg cagaaaaacg
    10261 tccaaatgga catcttcctg ggacatttaa agttgaattt ataaatgcct tatatgaaat
    10321 tacagatgaa gatgtcaaga aattcaaaag agtgaagtaa gatgtttcat aaagaattac
    10381 taaaactata ttttatttgt ggaacgacta cttgccaagg aaaaaatcta tatacagtcg
    10441 ttgaggaagc cttaaaaggt ggtataacct tatttcaatt tcgagaaaaa ggtgagggtg
    10501 ctttagaagg cttagaaaaa cttgagctag ctatccaaat taaagaactt tgtaaaaaat
    10561 acaatgttcc atttatcgtt aatgacgata tagatttagc aatggaaatt gacgccgatg
    10621 gcgtacatgt aggtcaagat gatattggtg ttgatgaaat tagaaaattg atgccagata
    10681 aaataattgg tctttctata agaaacgaag aagaatttca acaatcaaaa gttgaatatg
    10741 tagattatgt aggtgttggg cctgtatttg atacccagtc aaaatatgat gctggtggtg
    10801 ctataggtta tgaaggtctt gaattgatga gaaaactatt gccacaaatg cctttagttg
    10861 caattggtgg gatacagacg aaacatatta aagacattat aaagaccaat atggatggcg
    10921 tttcaatcat ttcggcaata tcgtatgcaa aaaatataga aaaaactgtt cgggaaatga
    10981 gtgaacaata gatatctatc cttggattct aagtaagtag gattttcctt ttttaaaaag
    11041 ctatgtccag attcagcttg aatggctaat tcatcaaaca ggtcatttgt tttttgcaaa
    11101 agaaagatct gctgtctttg tgagtgcaag gctgtatttc cagttcaatt tcttgaacta
    11161 gtataaaatg tgtgttataa taatcacata attaaataat aaagacattt ggggtgcttt
    11221 aggctgagat gatacccatt gaacctgata cagttaagac tggcgaaggg aaatgtgaaa
    11281 tgtttttttc gtatgtcgaa aagaactgtc taatcttgta agctgaactc taattcgtat
    11341 ctgtaattag agttttttct tttacgaaac tggatgaaga tgatatagta gattgaaata
    11401 agatgcgaac aaatcaatta gaaaattcaa attaatttct agaaatattt tagtatccct
    11461 gtgtactgtt ctaaattcag tctgctatat cttatttttt ctatttaaat cgcttctgta
    11521 acaaagctac gactttcaag taccttaagc atggcattag ctgtatctag cgctgtgaag
    11581 aggggcaccc cgtgttcaat ggctgaacga cgaatttgct caccatcttc gtcagcagtt
    11641 cgttttgttc ctactgtgtt aatgatagct tgaattcttc ctttgcgtac aaaacttggg
    11701 atatccttat cgtcatcacc aatcttacca acaggttggg cttgcaagcc atgactagca
    11761 aagaaggctg ctgtcccttc tgtcgcaagg attccataac caatgttttg gaaacgacga
    11821 gccaagttca aggcttcttc tttggcatca tcagcgatgg taaagacgac attaccaaaa
    11881 gttggcaagt gtagataaga agcttcaaag gctttataga gagctttttc caaagtagca
    11941 tcagaaccca taacttcacc tgttgacttc atttcaggac cgagcaagct gtctacctta
    12001 gctagtttgg taaaggagaa gacaggtgcc ttgatatgaa cacgggtgct ttcagggtaa
    12061 agtccatttt ggtagccaag ttctgataaa ctttgaccaa gaatgagttt ggtcgctact
    12121 tgagccatag gaatattggt taccttagat aggaatggaa cagtacggct ggcacgtgga
    12181 ttgacctcaa taacgtagac tttttcatcc ttgataacaa actggatgtt catcattcca
    12241 aggcagtgaa gaccgattgc taagcgtttg gtgtagtctg cgatggtctc ctgaaccttt
    12301 tgcgacaagg tttgtggtgg gtaaacagcc attgagtcac ctgagtggac accagcacgt
    12361 tcgatatgct ccatgatacc aggaatgagt acatttttac catctgaaat ggcatcaact
    12421 tcgcactctt gcccaacgat ataagagtcg acaagaactg ggtggtctgg actagcctta
    12481 acagcagttc gcatgtaaga acgaaggtct tcttcgtttt caacgatttc catggcacgt
    12541 ccaccaagta cataagatgg gcggacaaga actgggaagc caatcttacg agctgcaaga
    12601 gctgcttctt cttcattggt agccgtttgt cctggtggct gtggaatatc caattctttg
    12661 agagcttgct cgaagaggtc acggtcttcg gcacgatcta ggtcagcaac ctgtgtacca
    12721 aggatggtca cacctgcttt tgccaatggc tccgcaaggt tgatggctgt ttgaccaccg
    12781 aactgaacga taactccctt tggttgttcc aagtcaatga cgttcataac atcttcgaat
    12841 gtcaatggct caaagtaaag cttatctgat acagagaagt ctgttgaaac ggtctctggg
    12901 tttgagttca tgatgatagc ttcataacca gctgcctgga tagccttaac agagtgaacg
    12961 gttgcgtagt caaactcaac cccttgaccg atacggattg gacctgaacc taggacaagt
    13021 acagattctt tatcagatct gatagattca ttttcccaac cataggttga atagaaatat
    13081 ggcgtttcgg agtcgaactc tgccgcacaa gtgtctacca tcttataaac tggaacaatc
    13141 ttgttttcca agcgaagttg gcgaacttta tcatcagtcg ttccccagag ttcagcaatc
    13201 ttacggtctg aaaaaccatt aagtttggct gttttcaaaa cttctaaatc ttgtggatga
    13261 gcacccaatt cttgctcaat ttcaaagata tgcaagagtt tatcaagata gaagatatca
    13321 atttttgtaa gctctgcaat ttcttcaggt gtgtagccac gacgaatggc ttctgatacg
    13381 tagaagagac ggtcatcttg ggctttgaca accttttcaa tcaaggtatc atcagaaact
    13441 gctgcaagtt caggtatttc attgtggtgc accccaattt caagggagcg gcaggccttg
    13501 agaagagatt cctcgatgtt acgaccgatt gccatgactt ctccagtcgc cttcatttgt
    13561 gtaccgagac ggcgttcacc cttttcaaac ttgtcaaatg ggaaacgtgg aatcttagca
    13621 actacgtagt caagggctgg ttcaaacatg gcataggttg aacctgtaac tgggtttata
    13681 acctcatcca aggtcaaacc tactgcaatc ttggcagcca acttagcaat cggatatcct
    13741 gtcgctttag aagcaagggc tgacgaacgt gatacacgag ggtttacttc gataacataa
    13801 tacttgaagc tgttaggatc aagagctagc tgaacattac atccaccttc aatcttgagg
    13861 gcacgaataa tgctcaagct cgcatcacga agcatttggt tttcatagtc tgacatggtt
    13921 tgcgcagggg caaatacaat ggaatccccc gtgtgaatcc caactgggtc aaagttttcc
    13981 atgttacaaa caaccaaggc attgtcagct gagtcacgca tcacttcgta ttcaatttcc
    14041 ttgaaaccgg caatcgaacg ctcaatcaaa cattgggtaa caggtgacaa tttcaaacca
    14101 ttttcagtga tttcacgcaa ttctttctcg ttggcacaca taccaccacc agtaccacca
    14161 agggtaaagg ctggacgaac gatgactggg tagccaattg tcgctgcaaa ggcaactgct
    14221 tcttctactg tgttaacaat ttcagattct ggaatgggtt gctcaagctc ttccatcaat
    14281 tgtttaaaga gatcacggtc ctccgcttgg tcaatggcag ataatttggt acccagaagt
    14341 tcaacgccaa gctcgtctag gataccattt ttagataatt ccatggccat gttgagacct
    14401 gtctgaccac cgagtgttgg tagcaaggca tctggacctt ccttacgaag aatacgtgtc
    14461 acaaactcaa gtgtaatcgg ttcaatgtaa accttgtcag caatttcctt gtccgtcatg
    14521 atggttgcag gatttgagtt aaccaaaaca acctcataac cttcctcttt caacgacaag
    14581 caagcctgag tcccagcgta gtcaaactca gcagcctgac caataataat cggaccagaa
    14641 ccaatcacca taattttttg aatatcagta cgtttaggca tatataagat attaagggtg
    14701 tcaagcggac aaagctaaaa taggagttat gacgaagaac tgtcagttct aggaataact
    14761 atctttttag caccgtccgt agcccgtatt cagttcagca aatacggagc tcccttctcc
    14821 tttctattcg tcgcctctca gggcgacatt aaataagata caaaggacga atagaaagcg
    14881 attgaatttt aggaaatcaa ggaaggattg acaatccaag ttggtttctc tacattctga
    14941 gctttccgtc cgtgttcagt tacataaatt ctccgacgag cttttactcg ttcttagttt
    15001 gattgtttaa aaacttccat catctcgata aactcgtcaa ataggtagct agcgtcgtgt
    15061 ggcccaggag ctgcatctgg gtggtattga acagagaaag caggttggta tctgtggcgc
    15121 acaccttcca ctgacttgtc attgatttct tcgtgggtaa taatcaagtg ctctggcaaa
    15181 tcctcgcggc tgactgcata accatggttc tggctggtga agtctactcg tcctgttgcg
    15241 atttcacgta ccgcatggtt gaatccacgg tggccaaact tcatcttata ggtcttagcc
    15301 ccgtttgcca ttgcaaagag ttggtgtccc atacaaatac caaagattgg aatttttcct
    15361 tgtacaccgc gaatcatgtc gagtgcttgt ggaacgtctt ctgggttacc tggaccattt
    15421 gacaacataa ctccgtcagg attgagatgg agaatttctt cagccgttgt cgaataagga
    15481 acaactgtca cgttacagtt gcgtttagaa agttcacgta ggattgagtg cttgagacca
    15541 aagtccacta gcaccacgct caaaccaact cctggagctg gataagacgt tttagtagaa
    15601 acctgtttga tattgtctgt cggtaaaact gttgcttgga gctggtccgt cacatggtcc
    15661 atactgtccc caacatgggt caaggttgca cgcatagtac catgcttacg gataatcttg
    15721 gtaagagcac gcgtatcaat tcctgaaatc cctggaattt tcttggcttt caaaaattca
    15781 tccaaggtca tttggttgcg ccagttgcta gctctacgcg cttcttcaaa aacaacgact
    15841 cccttacaag ttggaataat ggattcataa tcatcacgat taataccata atttcctacc
    15901 aaaggataag taaaggtcaa gatttgtcca ttataagact ggtctgtaat ggattcttgg
    15961 tagccggtca tccctgtatt aaagacgatt tcgcctgtta catcaatatc tgctccgaag
    16021 gccttgcctt caaaaactgt gccatcttct aatactagaa ttctttttgt catattttca
    16081 cctctcgtgg acgctcactg gcgtctttta acgtcttgtg ttttagttgg cgtttctact
    16141 cgctagtacg gattctaaga ttgccattcg aacaaagaca ccattggtca tttgttggac
    16201 aatccgtgat tttggtgctt caaccaagtg gtctgctatt tctacatcac gattgattgg
    16261 agctgggtgc atgaggattg ctgtttcttt caaacgatcg taacgttctt gagtcaagcc
    16321 atgttgggca tggtagtctt cttttgaaaa tacagctcca ctatcatggc gttcgtgttg
    16381 cacacggaga aacatcatga catcaacctg atcaatgatt tcatcaatgg ttacaaactg
    16441 tccatagtct gcaaactctt gacttctcca ttcctcaggt ccagcgaaaa agagttcaga
    16501 tcccaagcgt ttcaaaatct gcatattgga tttggcaacg cgtgagtggt ccaagtcacc
    16561 tgcaatagca actttaagac cctcaaagtg gccaaattcc tcataaatgg tcatcaaatc
    16621 aagcaagctc tggctagggt gttggcccga accatctcca ccattgatga tggaagtcgt
    16681 aatcgttgga ctagcaatca attctctata gtagtcgacc tctggatggc gaatcacaca
    16741 gacatccact cctaaagcag acagagtcaa aatggtgtca taaagtgtct cacccttatt
    16801 aaccgaacta gtcttcacat caaagtcaag tcgttccaat ccaagtttaa tctctgcgac
    16861 ttcaaaggac ttatgtgtcc gtgtagaatc ctcaaagaag agattggaaa caatcggatg
    16921 gtcttcatag ggaagctggg ctccattttt aaactcaatt cctcgcttga tcaatttcat
    16981 tacttgatcg acagtgaggt cttccatgga caccacatgg ttcaatgctt gttgattttc
    17041 tgacatggct actcctttaa ctttctaagc ttcttcagta atcagaactc tgtcttggtc
    17101 atcaagttct gtcatctcta cgatgatttc ttcagaacga ctggttggga tattttttcc
    17161 aacgtaatct ggacggattg gcaattctct atgtccacga tcgactagaa ctgctaaact
    17221 cacacgcgca ggacgaccat gaccgacaat attatcaata gcagcacgga tggtacgacc
    17281 tgtatagagc acatcatcca ccaagataac ttcgcggtct gtcacatcga cagaaaccaa
    17341 agaagtatct tctccacttt taacatcatc acggaaaggt ttagtatcca attccacaac
    17401 aggaactgaa agattttcta actgcttcaa acgttcttgg attcggtggg caataaagac
    17461 accacgagtt ttaataccag ccaagacgat cttattcaaa tctttgttgc gttcgataat
    17521 ctcataagta atacgcgtaa tcgctcgttt gacggtcaat tcgtctacaa cttcttttgt
    17581 tttcatgaca aacctccaaa aagaaaagtc tccttaaaca aggagacttg aaatttatag
    17641 ccaagcgagc cctactgcac acagtataga cttcaccctt ctactttatc gcgctccttg
    17701 cctgcctcac gggacaggtt taaaggaata tttagttatc atttactata gcacaaagca
    17761 tgcttaaaat caagcaaaaa gtttcaatgt agcatcttac aaattgctaa aatcatataa
    17821 ttgtgggtac tggtcacact ctggattttt tggatggcaa atggctcttc caaaataaat
    17881 catggcctga tgggcagcta accactgctc aggcggcaag atatccatga cccgcttttc
    17941 cacctcaagt ggcgtcgctg attttttgac aatatcgtgg tgtttgcaaa tacgctccac
    18001 atgagtatcc actgcaaagg ctggaatccc aaatcctaca ctcatgacaa cattggctgt
    18061 cttgcgacca acacctgcca aactctccaa ttcttcacgt gtctgaggga cttgaccatc
    18121 aaaatcgtct agtaactgtt gggcacattt tttaaggaat ttagctttat tccgatacaa
    18181 tcccaagcga gaaatatgtg aagcaatctc actctctgtc gctacagaca tagcttgggg
    18241 tgttggaaag gcaacaaaga gacctggtgt ggccttattt accgctgcat ctgtcgtctg
    18301 ggctgataac atgaccgcaa ccaggagttc aaaatgattg gtaaaatcaa gactaggctt
    18361 ggcatctggg aagagggcaa tgatttcttc tagcaccttt cgtgctcgtt tttttgacaa
    18421 gaccattatt catctccgtc aaatagtcct tgtaagccag caaaaggact gttttcttct
    18481 ttctttactg cttgttgagc ttggtattct tcctctgtca tgatttgcca gtcatttcct
    18541 gagacaaatc cttgaccagc ttcttcttca gccgtcaaga ccttgatagg aatgtttagc
    18601 aagatattgt ctgatacact ctcagcaagg tcaagcgcct cattttcgat gggcaagacc
    18661 aagtcatcat ctaaaatttc ttgatttaga tggttagttg cgccttccat gaaaacttcc
    18721 gtgactggat aagattcaac taactcaact ggctccatac tgcgactcga agcaagaaca
    18781 atggtataag atagttgata atctaagaaa tacatacggt cttcatattg tactttccca
    18841 actgcaagga tatcttttac atctaaaatt tcttgattac gtgcacgcag gtcatcaact
    18901 aaatctaacg tttgttcaaa gttcaaacct tcagactgct tacgaatttc ttgaatattt
    18961 aatttcatac ttcctccata aagatttact ctcttgatta taccatgaaa aggctacaaa
    19021 tcagcacacc aaactttgta attaaaattc agaattttaa catatttact atgacagttt
    19081 tgttttttag tgctatacta tagggaaaga gtacatcaga tcaaggagga tgctcacatg
    19141 gaagacaaga aactcattca actcctatcc aagttaaata aaagctacca aaactgtaaa
    19201 cagggtacgg cagatgatat tcgactacaa gagctgctaa acactactat gcaagagctc
    19261 aaaaaaacgg aaaagttgaa caacagtatc ttaattgatc ttgagaaatt ttaccaaccc
    19321 accagtcttc tgattggact gggtagccta aaactaaacg atcaagcacg cactgcttgg
    19381 cgaaactatg ataaattcca ttacgagcat atcaaacacg tactaagtct ctatggacct
    19441 gtttttggat tttagagcat agaatttcca gttttctgtt gacaaaattt ccttaaaggt
    19501 ataatataaa gatactaata ctcggaggta agggagacat gaacaactaa gtctatcaaa
    19561 taaagaacct ttatttagta gatcttgttt ttgtctcttt ttgtgtgctc ttttatgctc
    19621 tttttctggc atgttaatag agtttttttg acatagactt tgggctctac taggtaaagt
    19681 agagcttttt gttatgcact atgaacattc tagaaaggga aatcatatga taaaaatcaa
    19741 tcatctaacc atcacacaaa acaaagattt acgagatctt gtatctgacc taagcatgac
    19801 catccaagac ggggaaaagg ttgctattat tggtgaagaa ggaaatggca aatcaacctt
    19861 acttaaaatt ttaatggggg aagctttgtc tgatttcact atcaagggaa acatccaatc
    19921 tgactatcag tcactggcct acattcctca aaaacttccc gaggagctga aaaagaaaac
    19981 tttacacgac tacttctttt tagattctat tgatttagac tacagtatcc tctatcgttt
    20041 ggcggaggaa ttgcattttg atagcaatcg tttcgcaagt gaccaaaaga ttggcaatct
    20101 atcaggggga gaagctttga aaattcagct tatccatgag ttagccaaac cctttgagat
    20161 tctattttta gatgaacctt caaatgacct agaccttgag acagttgatt ggctaaaagg
    20221 ccagattcaa aagaccaggc aaaccgttat tttcatttcc catgatgaag actttctttc
    20281 tgaaacagca gacactattg ttcacttgcg actggtcaaa caccgtaaag aagcggaaac
    20341 gctagtagag catttagact atgatagcta tagtgagcag agaaaggcta attttgccaa
    20401 acaaagtcag caagctgcta acaaccaaag aacctacgat aaaaccatgg aaaaacatcg
    20461 gagagttaag caaaatgtag aaactgcgct tcgagctacc aaagatagta ctgccggtcg
    20521 cctattggct aaaaagatga aaactgtcct ctcacaagaa aaacgctacg aaaaggcagc
    20581 tcagtccatg actcaaaagc cacttgaaga ggaacaaatc caacttttct tttcagacat
    20641 ccaaccatta ccagcttcta aagtcttagt ccaactggaa aaagaaaatt tgtccattga
    20701 cgaccgagtt ttggttcaaa aactacaact aactgtccgt gggcaagaaa aaatcggtat
    20761 tatcgggcca aatggtgttg ggaaatcaac tctgttagcc aaattaaagc aacttctgaa
    20821 tgataaaaga gagatttccc ttggttttat gccacaagat taccacaaaa aactgcaatt
    20881 ggatttatca ccaatagctt atatcagcaa aactggggaa aaagaggaac ttcagaaaat
    20941 ccaatctcac ctagccagtc tcaatttcag ttatccagaa atgcagcatc aaattcgctc
    21001 cttatctggc ggacaacagg gaaaactcct gcttttggat ttagtcctgc gcaaaccaaa
    21061 ctttctcctg ctggatgaac ccacacgaaa cttttctccc acttctcaac cccaaatcag
    21121 aaaactcttt gcaacctatc caggcggtct catcactgtt tcgcatgacc gtcgtttctt
    21181 aaaagaaatc tgctcgatca tctatcgcat gacagaacac ggtttgaagc tagttaattt
    21241 agaagattta taaatttgca acatagcaga taggtggggc aaaaacacaa ttttaggaga
    21301 aaaagaagta aatcttccca tagtaaaacg cataatatca agttttcaca cctgatatta
    21361 tgcgtttttc agattttaaa gacttttttc cagccttcat tttacatctg ttttaaacgt
    21421 tcaatccgtt ctgagatagg tgggtgggta taaaagagtt tttggaaccc cccacctttc
    21481 ttgggatcat tgatataaag agcactgcta gcatcatcga cgtggcgact cataggtttg
    21541 ctattgtcca acttatctag ggcattaatc attccctggg gattgcgagt cagctcgaca
    21601 ctagatgcat ctgccagaaa ttccctctga cgagaaatag cgagctgaac caaggttgca
    21661 gcgagaggtg ccagtacaat agctagtagg gaaaccacta gcataatgat ttcaagacca
    21721 tttccatctc ggtcatcatc acttcgtctg cgacctgctc caccccacca catcatacga
    21781 cctgccatac cagaaagcat ggtgatagca ctaacaaggg caactgcaat agtcgaaata
    21841 cggatatcat aattacgaat atgactgact tcatgtccca taacagcttc tagttcttca
    21901 cgattcatga tagctagtag acctgaagtc gcagcaacag ccgcattttg aggattagaa
    21961 cctgtcgcaa aggcatttaa ggctggatca tcaatgatga aaacacgggg cataggaatc
    22021 tgagcgacca gagccatatc ttccactaca tggtagaggt ctggtgccgt ttgctcatcc
    22081 acctcacgcg ctccattcat ggacatgaca atctctgtcg attgaaaaat catagacaaa
    22141 gcgtagataa agccgataat cagtgcgata accaaaccac caagtccaga tcttataaag
    22201 agataaccaa ccgcataacc aacaagagct aagagtagga aaaataccac caacaaaatc
    22261 caggtttttc gtttattgct tgcaatttga acaaacaaca tcttagtcac ctaaaccgct
    22321 aaaatcaact ttaggaaccg acttttcctc ttcaggtgtt tgaaggaaat ctgccgcttt
    22381 aaatccaaac attccagcga taatattgct cgggaaagtt tctaatttta tattgtagtt
    22441 gctgacaaca ctgttataga gttgacgaga gtaagaaatt ttattttctg tgtttgtcaa
    22501 ctcctcttgc aatttaacaa agttagcact agctttcaaa tctggatagc tttctgcaac
    22561 tgcaaaaata cctgaaacct gacgagtgag ggcatcactg gctttcatag cttctgctgg
    22621 tgaagtcgct gccgccactt ggttacgtag ttctgccacc ttttcaaggg tagaaccttc
    22681 atatttggca taacctttta cagtctcaat caagtttggc aagaggtcat ttcgacgttt
    22741 caactgaaca tcaatctgac tccaagcctc cttggtttgc atacgatttt taaccaaacc
    22801 gttatagcta acaatcacaa aaataacaat aagagcgata actccaagaa taatccaagt
    22861 cataatataa gtcctttctg cttttagatt agtaccagta tatcaaattt tctatgattg
    22921 tggtaaaata agatgatact aaagaaggaa ataactatga aaccaaaaac attttacaac
    22981 ttgcttgccg agcagaatct tccactttcg gaccagcaaa aagaacaatt tgaacgttat
    23041 tttgagctct tggtcgagtg gaatgagaag attaatttga cggcgattac ggacaaggaa
    23101 gaagtttatc tcaaacattt ttacgattcg attgcaccca ttcttcaagg tttgattccc
    23161 aatgaaacta tcaaacttct tgatatcggg gctggggcag gatttcctag tctaccaatg
    23221 aaaattctct atccggagtt agatgtgacc attattgatt cactcaataa gcgcatcaac
    23281 ttcctacaac tcttggctca agaactggat ttgaacggag ttcatttcta ccacggacgt
    23341 gccgaagatt ttgcccaaga caagaacttc cgtgctcaat atgattttgt aacagctcgt
    23401 gcggttgccc gtatgcaggt cctatctgaa ttgactattc cctaccttaa ggttggtggc
    23461 aaactattag cactcaaggc tagcaatgcg cctgaggaat tattagaagc taagaatgcc
    23521 ctcaatctcc tttttagtaa ggtcgaagac aatctcagct acgccctacc gaatagagat
    23581 ccgcgctata tcacagtggt agaaaagaaa aaagaaacac caaataaata tccacgtaag
    23641 gctggtatgc caaataaacg cccactttaa attttttagt aaacaaatgt ttactaaatc
    23701 agcctcgctc ttttatttct aggcttggga aaaaatgatt tacaaaatca gcctcgctct
    23761 tttatttcta ggctcgggaa aaaatgattt acaaaatcag cctcgctctt ttatttctag
    23821 gctcgggaaa aaatgattta caaaatcatt tttttctgct atactatcct aagcaaaggt
    23881 ttttaatgtc atcccgtgag gtgacgaaga cgcagaaata tttaaaactc tttaaaatct
    23941 aaattttaaa gaagtcttac tctgagggcc tattgctgta aaataatggg ctcttttttg
    24001 atgcccaaaa gtgaggttta tatgaaacaa gaatcaactg ttgatttgtt actagacgtt
    24061 gaccaacgtc cttcggctgg aaaaggaatt ctccttagtt tccaacacgt tttcgccatg
    24121 tttggtgcga ccatcttggt accattgatt ttgggaatgc ctgtatctgt tgcccttttt
    24181 gcttcaggtg ttggaacact catctacatg attgctactg gttttaaagt tccagtttat
    24241 ctaggttctt cattcgcctt tatcacagct atgtcactgg ctatgaaaga aatggggggg
    24301 gatgtatctg ctgcccaaac aggggttatc ttgactggtt tggtctatgt ccttgttgct
    24361 accagcatcc gatttgtagg aacaaaatgg attgataaac tcttgccacc aatcattatc
    24421 ggtcctatga tcatcgttat cggtcttgga cttgcaggtt cagctgttac caatgcaggt
    24481 cttgtagcag acggaaattg gaaaaatgct ctggtagccg ttgttacttt cctaattgct
    24541 gcctttatca atacaaaagg aaaaggcttc ctacgaatca ttccattcct ctttgccatt
    24601 atcggtggtt acctcttcgc actaactctt ggtttggttg actttacgcc tgtcctagaa
    24661 gctaactggt ttgaaattcc tggtttctac ttgccattta gcacaggtgg tgcctttaaa
    24721 gagtacaatc tttactttgg tccagaagcc atcgctatct tgccaatcgc tatcgtaaca
    24781 atttctgaac atatcggaga ccatactgtt ttgggtcaaa tctgtggccg tcaattctta
    24841 aaagaaccag gtcttcaccg tactcttatt ggtgacggta tcgcaacttc tgtttctgcc
    24901 ttccttggtg gaccagccaa tacaacttac ggagaaaata caggggttat cggtatgact
    24961 cgtatcgctt ctgtctcagt tatccgtaac gctgccttca tcgcgattgc cctcagcttc
    25021 cttggtaaat tcactgcctt gatttcaact attccaaacg ctgtacttgg tggtatgtca
    25081 atccttctct atggggttat cgccagcaat ggtttgaaag tcttgattaa agaacgtgtt
    25141 gatttcgctc aaatgcgaaa cctcatcatc gcaagtgcta tgttggttct tggacttgga
    25201 ggagctatcc ttaaacttgg tccagttaca ctttcaggta ctgccctttc agccatgaca
    25261 ggaatcatct tgaacttgat cttgccatac gaaaataaag actaagagtc taaatacacc
    25321 taatccactc agacagctga gtggattttt cgtataccat aataaaagtg tcttaacaaa
    25381 attattaaaa tcaaaaaacg tataatatca gatattctaa aaccttgata ctgtacgttt
    25441 tatcatagaa atttttactt tattttctca tcaaatgaga tttgcatcaa tctcttgtct
    25501 tacttgcgtt tcttcttcgc tttcttcatt ttgttagcca tacgtttcat ggactgtttc
    25561 atggcaaatt caccaatttt acctttcaaa ccgccaccaa acatctggct catatctggc
    25621 attcctgctc ctccgagagc tgataagtca ggcataccgc cttgtcccat cattccttca
    25681 agggcagaca tatccattcc tcccatattt ggcatatttt taggaaggtt atttggatta
    25741 atccccattt gcttcatcat tttattcata tccccagaca taacaccctg catgagctgt
    25801 ttagcctggt taaagtcctt gatgaattta ttgacttcga cgaatgtatt tccagaacca
    25861 gcagcaatac gacggcgacg gtttggattt aacaaatctg ggttttcacg ttcttcaggt
    25921 gtcatcgaag acacaatggc acgtttacga gcaatctggc gttcatccac cttcatgttt
    25981 tgaagtgctg gattgttggc catacctgga atcatcttga gcaagtcttc catcggcccc
    26041 atattttgca cctgatctaa ttgatcgatg aaatcattaa aatcaaaggt gttttcgcgc
    26101 atcttctcag ccatttcaag ggctttttgt tcatcgtatt cctgagaagc tttttcaatc
    26161 aaagtgagca tatcccccat gccaaggata cggctagaca tacggtctgg gtggaaggtt
    26221 tcgatatctg taattttttc acctgtacca gtgaacttga ttggttttcc agtgatgtga
    26281 cgaaccgata gagccgcacc accacgagta tcgccatcaa tcttggtaag gatgacccca
    26341 gtcacttcca actgagcatt aaactcacgc gcaacattgg ctgcttcctg accaatcata
    26401 gcatcaacga caagcaagat ttcatttggt tgagccaatg ctttcacatc acgaagctca
    26461 ttcatgagga gctcatcaat ctgcaaacga cccgcagtat caatcaagac atagtcgtta
    26521 tgattagttt gggcttgctc caaaccttga cgtacaatct caacagctgg tacttctgtt
    26581 ccaagtgcaa agacaggcac atcaatctgt tgtcccaagg tcttaagctg gtcaatggca
    26641 gctggacgat aaatatccgc cgcaatcatc aaaggacgag cattttcttc tttcttgagt
    26701 ttgttggcca atttaccagc aaaggttgtt ttaccagccc cttgtaaacc aaccatcatg
    26761 atgatggttg gaatcttagg tgacttgata atttctgccg tatcagaacc taaaacggct
    26821 gtcagttcct catcaacgat tttaataatc tgttgcgcag gattaagtgt atcaatgacc
    26881 tcatgcccga ctgcacgctc acgaactttc ttgataaagt cctttacaac aggcaaggca
    26941 acgtcggcct cgagcaaggc caagcgaatt tctttggttg cctcttggac atcagattca
    27001 gagatttttc cttttttacg tagattttta aagacgttct gcaaacgttc tgttaaactt
    27061 tcaaatgcca tttttcttcc tcttattctc tattatcaat gcttgttaaa atttctatct
    27121 gctcctgcag aaagtcatcc ttgggatagc gctccaaaat ctgatcaaaa atctgactgc
    27181 ggacaatata gtccgagtac atgtgcaatt tcatctcata atcttccaga atcttttctg
    27241 ttcgcttgat attgtcatag acagcctgac gactgacacc aaactcctcg gcaatttcag
    27301 caaggctgta atcatcagcg tagtagagct cgatataatt catttgctta tctgtcaaaa
    27361 gcgccgcata aaattcaaag agcgcattca tacgattggt tttttcgatt tccataactt
    27421 ttattatacc aaaaattagc ctaatctacc acactaggaa gccgatccaa gaagatagat
    27481 agctaaattt gaaaaagaca tgagcctagc cccaagtaat ttccaattga tagctggcaa
    27541 aaggatgtcc ctcttgattt tgtagttgat aatctagttc aatcttttgc ctatcaactt
    27601 gataatggct cgtttggatg ataaactcct gcatgcccat aggtgtagga atataggcta
    27661 aactatcgct atcctttaga aagcgcataa tggtcttggg attagaaaat cggctcatca
    27721 caagttcttg accatgaaat ttaatcacta ctttttcctt ttcctcatta tagaaaagca
    27781 ggtagctata atctcctttt tcatgcactt ccacatcata aagctggtca atcacttcca
    27841 actgctcatc aaactgaatt gtatttcgca tccgaatctt cacatcaggc cctctttctt
    27901 gtctcttgtc ctactatttt accaaaaaga gcaggatttt gctataatgg tcatatgaac
    27961 gaaaaagtat tccgtgaccc agttcacaac tacatccatg tcaataatca aatcatctat
    28021 gacttgatta atacaaaaga atttcaacgt ttgcgccgta tcaagcaact gggaacctcc
    28081 agttatacct tccacggtgg agagcacagc cgcttctctc actgtctagg agtctatgaa
    28141 attgcacgac gcatcacaga gattttcgaa gaaaaatatc ctgaggaatg gaatcctgcc
    28201 gagtctctct tgaccatgac cgctgctctc ctacacgacc ttgggcatgg tgcctactcc
    28261 catacttttg aaaatctctt tgatacagac catgaagcca ttactcagga gattattcaa
    28321 aatcctgaga cagagattca ccaagtcctg ctacaagtgg cacctgattt cccagaaaag
    28381 gtggccagtg tcattgacca tacctatcct aataagcagg tcgtgcagct catttctagc
    28441 cagattgatg cggaccgcat ggactatctc ttgcgtgact cctattttac aggagcatcc
    28501 tatggggaat ttgacctgac tcgaatcctc cgagtcattc gtcctatcga aaatggtatc
    28561 gcctttcagc gcaatggcat gcacgccatc gaagactacg tcctcagtcg ctaccagatg
    28621 tacatgcagg tttatttcca ccccgcaaca cgcgccatgg aagttctcct acagaatctt
    28681 ctcaaacgcg ccaaggaact ctatcctgaa gacaaggact tctttgcacg aacttctcca
    28741 cacctcctgc ctttctttga aaaaaatgtg accttgactg actatctggc tctagatgat
    28801 ggtgtgatga atacctactt tcaactctgg atgaccagtc ctgacaagat tctcgcagac
    28861 ttgtcacaac gctttgtcaa ccgcaaggtc tttaaatcca ttaccttttc acaagaggac
    28921 caagaccaac tcgccagcat gagaaaatta gctgaggata tcggctttga tcccgactac
    28981 tacactgcca ttcataagaa ctttgacctc ccttatgata tctatcgtcc cgaatctgaa
    29041 aacccacgga cacagattga gattttacaa aaaaatggag aactggccga actctctagc
    29101 ctgtctccta tcgtccaatc ccttgctggc agtcgccacg gagataatcg tttctatttc
    29161 cctaaagaaa tgttagacca aaacagcatc ttcgcaagca ttacccagca atttttacac
    29221 ttgattgaga acgatcattt taccccaaat aaaaactaga agaggaaatt tatgagtatt
    29281 aaactaattg ccgtcgatat tgacggaacc ctagtcaaca gccaaaagga aatcactcct
    29341 gaagtctttt ctgccatcca agatgccaaa gaagctggtg tcaaagtcgt gattgcaact
    29401 ggccgcccca tcgcaggtgt tgccaaactt ctggacgact tgcagttgag agaccaaggt
    29461 gactatgtgg tgaccttcaa tggtgccctt gtccaagaaa ctgctactgg ccatgagatt
    29521 atcagcgaat ccttgaccta tgaggattat ctggatatgg aattcctcag tcgcaagctc
    29581 ggtgtccaca tgcatgccat taccaaggac ggtatctata ctgcaaatcg caatatcgga
    29641 aaatacactg tacacgaatc aaccctcgtc agcatgccta tcttctaccg tacccctgaa
    29701 gaaatggctg gcaaagaaat tgttaaatgt atgtttatcg atgaaccaga aattctcgat
    29761 gctgcgattg aaaaaattcc agcagaattt tacgagcgct actccatcaa caaatctgct
    29821 cctttctacc tcgaactcct taaaaagaat gtagacaagg gttcagccat tactcacttg
    29881 gctgaaaaac tcggattgac caaagatgaa accatggcaa tcggtgatga agaaaatgac
    29941 cgtgccatgc tggaagtcgt tggaaacccc gttgtcatgg aaaatggaaa tccagaaatc
    30001 aaaaaaatcg ccaaatacat caccaaaaca aatgacgaat ccggcgttgc ccatgctatt
    30061 cgtacatggg tactgtaaaa ggaaagaata gactaaaacc gctcagttga gcggtttttg
    30121 atgtctaatt actgccccct gcaggaatcg aacctgcaac tactccttag gagggagttg
    30181 ttatatccat tgaactaagg gagcttgata aaaactctgc tgaaaagcag agttttttag
    30241 tcgaattaac gacggatttc tttgatacga gctgctttac cttgaagagc acgcaagtag
    30301 tacaatttcg cacgacgtac tttaccgtaa cgaacaactt cgattttttc aacacgtgga
    30361 gtgtggattg ggaagatacg ctcaacacct acaccgttag agattttacg aactgtgtag
    30421 ttttctgaga ttccagcacc tttacgtgcg ataacaacac cttcaaaaat ctggatacgt
    30481 tcacggttac cttcgacaac tttcgcatgt acacgaacag tgtcaccagg acggaatgat
    30541 gggatatctg tacgaagttg accttcagtc aagctttgga ttaatggatt cattttattc
    30601 tcctatcttt gtcaatcttg aggaaacttc ctcagcggat aaactgtatt tttgtgcgtc
    30661 catcacacac aagatacagt ctaccaaatt ttcacaaaaa agtaaagaaa tttatagcaa
    30721 aattcctaaa aaaatcgcaa ccaaaccacc tatgtaagtc aaagtcaggt aagaataaaa
    30781 gaccttctta tcacttagca gtctttgaag ttcgtcattc aaggtcgaaa aagttgttaa
    30841 accgccacaa aatcctgttg ctagaatagc atatacttcc ttagactcta catgattgta
    30901 gaatactcca attaaaaaac aacctaaaag attagctatg agcgttccga gtggcaattt
    30961 agaagcttga ttgtagcggg aaaagaaata ccgtacgagt gctcccaagc cacaggcgat
    31021 tgcaagatag acgattacca tttcttcctc cccaaatagt aagctaaaag caggcctcca
    31081 ccgatactca aaagcaaata cagtatcaaa ctaagataac gccctgtatc cagtaatttc
    31141 acagcatcaa gcatgagact agaaaaggtt gttaaacctc cgcaaaaacc tgtacccagt
    31201 gctaaaatca agcccttact agttccctta taaaccagat agcccttaac aagataaatc
    31261 aagcagaaaa tgcctagata gttgacaaga agggttcccc agggaaaatc tggactggct
    31321 ggtaaccaaa tggaaaccag atagcggata agtccgccca acatggcagc tataaaaatg
    31381 cctagcggat aaaattgttc ttttttcatt tgatattctg atcctgataa tcacgcgaac
    31441 gtttgagtat atccgaaaac gttgcgacaa tagtctcctg atagcgcttg tcctctacac
    31501 gactcctaat tttttcaaaa ataactgttt ctctcttggt atctaaaatc ggtttacccg
    31561 aagctttctt ataagccaca acctcttcaa ctaagtgcat tcgttcttcc aacagtttga
    31621 caatttggtc gtcgatttga tcgatttctt gacgaataat atctaaatcc atacagtctc
    31681 ctcctttatt tgaattattg tatcaaaaag ccatcaatta ggctagtaaa atcccaactc
    31741 tcgataagaa aaatgcactg attgattaca tcagcgcact tttatgctta tcctacttta
    31801 gcagccaagt cttctgcgaa ttgttccaag cgttcaatat cttcttcctc ggcagaaaga
    31861 tcaactttga cacactcaga acctttttct gctcctgttg acacaaagat acggtcaaag
    31921 tcatcaacag ccttacagaa ttcatcgtag aaggtatctc ctgagccgac cactccgtag
    31981 attttgccat tcaagttgag atctgctagg tcttcgtaga agtccatcat ctcatctggc
    32041 aattcaccat caccataagt ataggtcgca acgatagcga tgtctgcttc caagaagtct
    32101 gaagcgtcaa cagttgtaca ttcatcaaca tcaacatcca agcccaagtc acgtaatttg
    32161 tctgctacaa tatctgcaat ttcttcggta ttaccggtca tactggcaaa tacaattttt
    32221 gctaatgcca tatcgtcctc ctcaatttat ctttctccat tatatcacat ctttttcatt
    32281 ttaaaaataa aaattcttgt gctacaatga aatgagaaag aattgaggtt atttatggag
    32341 atttgccaac aaattttaga aaaaattaaa gaatacgaca ccattatcat tcatcgtcat
    32401 atgaaaccag accctgatgc cttgggaagt caggtgggat tgaaagcctt gctggaacat
    32461 catttcccag aaaaaaccat caaagccgtc ggttttgatg aaccaactct tacttggatg
    32521 gctgagatgg atcttgttga agatagagcc taccaaggcg cacttgtcat cgtctgtgat
    32581 acagctaata ctgctcgtat cgatgataag cgctatagtc aaggtgattt tctcattaag
    32641 attgaccacc atccaaatga tgatgtatac ggtgacctgt cttgggtcga tactagttca
    32701 agtagcgcta gcgagatgat taccctattt gcccaaacaa cccaactagc cttggcagat
    32761 cgcgatgctg agttgctctt tgcaggaatt gtcggtgata caggtcgctt cctctaccct
    32821 tctaccactg cacggactct tcgcctggct gcttatttga gagaacataa ctttgacttt
    32881 gcggctctca ctcgcaaaat ggacactatg agctacaaaa ttgctaaact gcaaggctac
    32941 atctacgacc atctggaagt ggatgaaaat ggtgctgctc gcgttatcct gagtcaagaa
    33001 gtcttgaaac aatacaatgt aaccgatgct gaaactgcag ccattgtcgg tgcacctgga
    33061 cgcattgaca gagtgagtct ctggggaatt tttgtcgaac aggctgatgg ccactaccga
    33121 gttcgcttac gcagtaaaat ccatcctatc aatgaaatcg ccaaggagca tgatggtgga
    33181 ggccaccctc tagcaagtgg tgctaattcc tatagcctag aagaaaatga aatcatctac
    33241 caaaagttaa aaaacttgct taaaaactga taaaatactt gccaaacttt tcagaatctg
    33301 atagactagt atggtaacaa tctatggctc gcaaagagac catggcagaa aggaaatatt
    33361 gcaaaatgaa aaaagatatc catccagaat atcgcccagt tgtcttcatg gacacaacta
    33421 ctggttacca attccttagc ggttcaacaa aacgctctaa cgaaacagtt gagttcgaag
    33481 gcgaaactta cccattgatc cgtgtggaaa tttcatcaga ctcacaccca ttctacactg
    33541 gacgtcaaaa gttcactcaa gcagatggac gcgtggatcg tttcaacaaa aaatacggtc
    33601 tcaaataatg ataagaggac agtttcggct gttctttttt gtttcttgaa atcaactgct
    33661 gttttcatgt tccagactca tctgtaggtt cgatttccat gctactaggc aggaaggaaa
    33721 tagctgtttc aacacgtcca taatgagctg tactattgtc acgaaccata ctttcattga
    33781 tggtccaagt ggaattcatt ttcttaaagg cctctcgaac cttttccaaa tctttggagg
    33841 caatggcctg ctctaaggtt tcaaaacgag gacttagacg gtttaccaat ttttcctttt
    33901 ccgcatctag gtcaacagga ttttgttctt tttcaaaagc taatagagct gaggaaattt
    33961 cggtgagttt ggcttcagta atatctccag atagagctaa cttttcctta acaatcttac
    34021 caacctcaga atccttattt tctactcttt cgaagtctga tgccatcttc gtaacgagtt
    34081 tctgggtctc agtctgacca ccatttttaa cagctttgga tgcatctgtg attttcacaa
    34141 agaaaaaact caagctttct tgggcacctg ctggggaaag aattaaaaat gacaaagcta
    34201 aaactagtaa ccagctttta gccttccaat aatttctgac caatgtagcc tcctttctcc
    34261 acaccaccaa agcaagtaaa aagtccacta cctatgtgag tgatgtattc attcatctta
    34321 tcagtagcac ctaggttagt ttgaacctta acaaagttat caggatcctt ctggaaagaa
    34381 atgaagagca atcccgtatc aaactgacca gtcttttcat caatgccatc tgagtaagag
    34441 taagaacaac gcaaaagtgg cttgtccact tacttagcca aacgaacatg cgaatcatct
    34501 agcaaaaaac tcaaattcac ttcatcaaac tcattttttt accgaaaggg gcactacttt
    34561 ccttgtaacg accaaaggta ttttcttgtt cttcgagacc agtgcggtcc caggtctcta
    34621 aaaacatctg aatccgacga actgccatat aagaaccatt ttccatccag tccttactat
    34681 cagcccagat aacgcggtca aagtcttgct ccttggttgg atttgctgtt ccatccttaa
    34741 aaccaaagag gttccacgga gtctccattc gtccccgata gctgcaaatc cagactgact
    34801 ccaacgaagg ggcaccgcat ttctcccctt acgtatgaga ttgcgaataa cgtgaaaggc
    34861 aatttgctca tcatctgcac aagcatgaat cacaatatct ccaccagtat atttttcacg
    34921 taattgctcc ttaggaaata gaggtaaatc cctgaacagt cgagggcgct tgttttccaa
    34981 gttcatcttt ttcaagaaac ttgcagatac accaaaagtc agcgtcaaac gatgaggatt
    35041 gagcccaact gtctcaccag tatcgctcgg aggaaagaga gcattctgtc catctttttt
    35101 aaccaattcc ccctcgacca acttggcact ataatccgtc caatccttga atagctggat
    35161 aattttatct ctatccgtcg tatgcaggtc caagacaaca aaatagatat tcttttgcat
    35221 gggcgtacta tttggtacat caagcgaacc ttacgaaaac gtctactata tttaaaccgc
    35281 tttctt
//
LOCUS       DAVKQL010000016        35236 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVKQL010000016 DAVKQL010000000
VERSION     DAVKQL010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35236
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.016"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(44..608)
                     /locus_tag="VW561_000712"
                     /pseudo
     CDS             complement(44..608)
                     /locus_tag="VW561_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(684..896)
                     /locus_tag="VW561_000713"
     CDS             complement(684..896)
                     /locus_tag="VW561_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9943002.1"
                     /translation="MIQPLLHADETSYRVLENDSHLTYYWTFLSGKAENQAITLYHHD
                     QRRSGSVVQEFLGDYSGYVHCDMLRQ"
     gene            complement(905..1210)
                     /locus_tag="VW561_000714"
     CDS             complement(905..1210)
                     /locus_tag="VW561_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HEU9943003.1"
                     /translation="MFPVETEEITYKRKKSKGKRQAILAQFDSEEVHHQVEESICPDC
                     QDDLKEIGASLQRQELVFIPAQLKRVDHIQHAYKCQTCSKNNPSDKIVKAPIPKAPL"
     gene            complement(1197..1391)
                     /locus_tag="VW561_000715"
     CDS             complement(1197..1391)
                     /locus_tag="VW561_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9943004.1"
                     /translation="MKIIQQQSAIIDSLTNELSLLCEQVAYLTQKLSGKSSEKSVCPF
                     GQLNLFEEESPSEKDGDVPS"
     gene            complement(1561..1911)
                     /gene="tnpB"
                     /locus_tag="VW561_000716"
     CDS             complement(1561..1911)
                     /gene="tnpB"
                     /locus_tag="VW561_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU9943005.1"
                     /translation="MTIHLSSLGQVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWLLYKRFENGRLTWPSTEKDVKALTPEQVDWLMKGFS
                     ITPKINLSESRDFY"
     gene            complement(1892..2077)
                     /locus_tag="VW561_000717"
     CDS             complement(1892..2077)
                     /locus_tag="VW561_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943006.1"
                     /translation="MPQPIVPVEIPQSRPFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MVEQLLDKVLLYDNSSI"
     gene            complement(2381..3943)
                     /gene="guaA"
                     /locus_tag="VW561_000718"
     CDS             complement(2381..3943)
                     /gene="guaA"
                     /locus_tag="VW561_000718"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEU9943007.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            4085..4783
                     /locus_tag="VW561_000719"
     CDS             4085..4783
                     /locus_tag="VW561_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9943008.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQISYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(4826..5722)
                     /locus_tag="VW561_000720"
     CDS             complement(4826..5722)
                     /locus_tag="VW561_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU9943009.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAIFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(5731..6666)
                     /locus_tag="VW561_000721"
     CDS             complement(5731..6666)
                     /locus_tag="VW561_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEU9943010.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(6663..7388)
                     /locus_tag="VW561_000722"
     CDS             complement(6663..7388)
                     /locus_tag="VW561_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEU9943011.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDIASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(7472..8107)
                     /locus_tag="VW561_000723"
     CDS             complement(7472..8107)
                     /locus_tag="VW561_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEU9943012.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEREEYQQAVYIRSNEKIQNWNQAYQDL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKSLKDYLY"
     gene            complement(8109..8936)
                     /locus_tag="VW561_000724"
     CDS             complement(8109..8936)
                     /locus_tag="VW561_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9943013.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALTHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(8958..9884)
                     /locus_tag="VW561_000725"
                     /pseudo
     CDS             complement(8958..9884)
                     /locus_tag="VW561_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809127.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
     gene            10535..11146
                     /locus_tag="VW561_000726"
     CDS             10535..11146
                     /locus_tag="VW561_000726"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU9943014.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRTVAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            11191..11919
                     /gene="rlmB"
                     /locus_tag="VW561_000727"
     CDS             11191..11919
                     /gene="rlmB"
                     /locus_tag="VW561_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEU9943015.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(11958..12869)
                     /gene="trxB"
                     /locus_tag="VW561_000728"
     CDS             complement(11958..12869)
                     /gene="trxB"
                     /locus_tag="VW561_000728"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEU9943016.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLIVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDSHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(12935..13159)
                     /locus_tag="VW561_000729"
     CDS             complement(12935..13159)
                     /locus_tag="VW561_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEU9943017.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(13237..13980)
                     /locus_tag="VW561_000730"
     CDS             complement(13237..13980)
                     /locus_tag="VW561_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9943018.1"
                     /translation="MIKISNLSKSFSGQTVLDHLNLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMPFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(13980..14780)
                     /locus_tag="VW561_000731"
     CDS             complement(13980..14780)
                     /locus_tag="VW561_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9943019.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(14938..15294)
                     /locus_tag="VW561_000732"
     CDS             complement(14938..15294)
                     /locus_tag="VW561_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEU9943020.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(15288..15818)
                     /locus_tag="VW561_000733"
     CDS             complement(15288..15818)
                     /locus_tag="VW561_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU9943021.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDRSNRRRSTC"
     gene            complement(15818..16312)
                     /locus_tag="VW561_000734"
     CDS             complement(15818..16312)
                     /locus_tag="VW561_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9943022.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            16447..16893
                     /locus_tag="VW561_000735"
     CDS             16447..16893
                     /locus_tag="VW561_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEU9943023.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTIKLYHQTLDLIDHTQEEVIQ"
     gene            16890..17537
                     /locus_tag="VW561_000736"
     CDS             16890..17537
                     /locus_tag="VW561_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEU9943024.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASVLQYIAIVYYM"
     gene            complement(17558..18139)
                     /gene="pdxT"
                     /locus_tag="VW561_000737"
     CDS             complement(17558..18139)
                     /gene="pdxT"
                     /locus_tag="VW561_000737"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEU9943025.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(18140..19015)
                     /gene="pdxS"
                     /locus_tag="VW561_000738"
     CDS             complement(18140..19015)
                     /gene="pdxS"
                     /locus_tag="VW561_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEU9943026.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(19167..20546)
                     /locus_tag="VW561_000739"
     CDS             complement(19167..20546)
                     /locus_tag="VW561_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU9943027.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVITAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            20840..21763
                     /locus_tag="VW561_000740"
     CDS             20840..21763
                     /locus_tag="VW561_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEU9943028.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            21823..22428
                     /locus_tag="VW561_000741"
     CDS             21823..22428
                     /locus_tag="VW561_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9943029.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKTIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            22446..23690
                     /locus_tag="VW561_000742"
     CDS             22446..23690
                     /locus_tag="VW561_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9943030.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRNFKFKFELEVLEIDEVPMF
                     NQDEKWDESFQLRFLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNHGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGDHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(23913..24170)
                     /locus_tag="VW561_000743"
     CDS             complement(23913..24170)
                     /locus_tag="VW561_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEU9943031.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(24212..26248)
                     /locus_tag="VW561_000744"
     CDS             complement(24212..26248)
                     /locus_tag="VW561_000744"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEU9943032.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQSVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEEEFDLDFLDIKGGRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(26508..27425)
                     /gene="glyQ"
                     /locus_tag="VW561_000745"
     CDS             complement(26508..27425)
                     /gene="glyQ"
                     /locus_tag="VW561_000745"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEU9943033.1"
                     /translation="MSKKLTFQEIILTLQRFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRVKLLAEDAE"
     gene            complement(27619..28380)
                     /locus_tag="VW561_000746"
     CDS             complement(27619..28380)
                     /locus_tag="VW561_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU9943034.1"
                     /translation="MENLYLVKDDSQLAAFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSNFNAATQIIRESAVPAYTNNRRMVMTPDLAVWKELYLYQLMDYECSQQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            complement(28653..29495)
                     /locus_tag="VW561_000747"
     CDS             complement(28653..29495)
                     /locus_tag="VW561_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU9943035.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQDQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     VAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTIAVQSGAPRVD
                     DVDF"
     gene            complement(29609..31000)
                     /locus_tag="VW561_000748"
     CDS             complement(29609..31000)
                     /locus_tag="VW561_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU9943036.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKHQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(31235..31612)
                     /locus_tag="VW561_000749"
     CDS             complement(31235..31612)
                     /locus_tag="VW561_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEU9943037.1"
                     /translation="MICQFIRDMLDLPAKNVTILEGSNIHVLPSMPYSAQDFYTSIDV
                     LAELDNGIQVIIEIQVHHQNFFINRLWAYLCIQVNQNLEKIRQREGDTHQSYKQIALV
                     YAIAIVDSNYFSDDLAFHSFIVK"
     gene            31827..32804
                     /locus_tag="VW561_000750"
     CDS             31827..32804
                     /locus_tag="VW561_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU9943038.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFEKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            32801..33883
                     /locus_tag="VW561_000751"
     CDS             32801..33883
                     /locus_tag="VW561_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9943039.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTINLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILTVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(33947..34977)
                     /locus_tag="VW561_000752"
                     /pseudo
     CDS             complement(33947..34977)
                     /locus_tag="VW561_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT        10444 a         7852 c         6325 g        10615 t
ORIGIN      
        1 tgttttagca gtcgatgcgt actgttatag attcaacgca ctataattga tgacgtttag
       61 ctgtttccaa caaactcata ataatagctc ttgctctagc tccttcaaaa ctttgagaaa
      121 ataaccaccc tcgctttttt aaactgagct taggctaaaa gagtccactg gactctttta
      181 ctccgtccca taaccaatga tttaatggcg cattcagcta gattattgga aaggaccaga
      241 tgtccgtctt tcaaaatagt cttaaaggtt tctttatact tgaggctata cttgaggctg
      301 tattcaattg cccttcctag ttttgaaccc gataaaactg actgacgccg gcaccaagca
      361 aagaaatctt ccattagggg ttggagctct tcttgacgtt tctgtagtcg ttcatcagct
      421 ggcaaagcct cccagtttct ttccaaggaa aataactgat tacaataagc taaaccttta
      481 gctcctaagg atgatttatt cgcttgcttg ggggtcgctt caaaaaactt ccttctcaca
      541 tgtacccaac atccaacaag ttcagcttct tccagttgac aataagcttc tagcggttcg
      601 cagtttacca agcttagcgt cgccttgctc ctaaaccttg gactgctatc gcataggcag
      661 aactagagga ctaaagtcct aagttactgc cgcaacatat cacaatgcac atagccagaa
      721 taatctccta ggaattcttg cactaccgaa ccactccgac gctgatcatg gtggtacagc
      781 gtgattgctt gattctcagc tttcccagac aaaaaggtcc aatagtaggt cagatggcta
      841 tcattctcta gaacccgata ggaggtttca tctgcatgaa gtaagggctg aatcaatagt
      901 ctcgttataa aggggcttta ggaataggag cttttacaat tttatcactt ggattattct
      961 tactgcatgt ttggcatttg taagcgtgtt ggatatgatc cactcttttt aattgcgcag
     1021 gaataaagac caattcttgt cgttgaaggc tcgctccaat ctcttttaaa tcgtcctgac
     1081 aatcagggca aatgctctct tctacttgat gatgaacttc ttctgaatca aattgggcaa
     1141 gaatagcttg acgtttccct ttagatttct tacgtttgta ggtaatttcc tctgtttcaa
     1201 ctgggaacat ctccatcttt ctcagatgga gactcttcct caaaaaggtt gagttgtcca
     1261 aaagggcaaa cacttttctc agaggatttt ccagagagct tttgcgttag ataagccact
     1321 tgttcacaca gaagggaaag ttcattagtg agactatcaa ttatagcact ctgttgttga
     1381 atgattttca atcttctccc atagactttc ctccttattt cctaacttta ttatatagtg
     1441 tattgaatct ataacagtac accttgactg ctaaaatatt tctataaatt aatttgactt
     1501 tcctgataga gatgttcata tcttatttca attcactata ctaaaaagaa agtcctcatt
     1561 tcaatagaaa tcacgacttt ctgataaatt tattttggga gtgatagaaa agcccttcat
     1621 aagccaatct acttgttcag gtgtgagagc tttgacatcc ttttctgtac tgggccaagt
     1681 cagtctgccg ttctcaaagc gtttatatag tagccaaaat ccttgaccat cccagtaaag
     1741 ggctttaaag cggtctttac gtccaccaca aaagagaaaa acttgaccgg agaaaggatc
     1801 caattcaaag tgggttttaa ccagataagc cagtgaatcg attccttgtc tcatatcagt
     1861 cttcccacac acgagataga cctgccctag gctagataga tgaattgtca tagagcaaca
     1921 ccttatccaa aagctgttct accatttcga gattgagaga ttgaaaaaaa cttacttcaa
     1981 gcttgccaat acgaatttta agcagaatat catttctctt tttagaatca aaaggacgag
     2041 attgtggaat ctctacagga acaataggtt gtggcattaa aatatcctcc aatagtttta
     2101 ctttactaat agtatacagg aggaactcca tgaatgatag ataccttgtt atgacgcgct
     2161 tactatatta gaaagttggc tctctaatta aattaattct cattatatat atcaaaagac
     2221 cgcagtttga aaactacggt ctaatttttt gtccaaatgt aaatcacttg gaactattat
     2281 tcaatttatt ctttttagta aatgttcccg ttttaatccc agttacagtt tttaatcctc
     2341 tgtaaccgtt gattttacaa agcttttaat ttttttgcga ttactcccac tcaactgttg
     2401 caggtggttt acttgtaata tcgtagacga tacggttaac atgatccact tcatttacga
     2461 tacgtactga gattttttga agtacttccc atggaatttt ggcaaaatca gcagtcatac
     2521 catcgataga agtgatagca cggattgcaa tcgtgtagtc atacgtacga ccgtcaccca
     2581 taacaccgac tgaacgaacg cctgtgttaa cagtgaagta ttgccaaata tcgcggtcaa
     2641 gtccagcttt agcgatttct tcacgaagaa tagcgtctga ttcacgaacg gtttcaagtt
     2701 tctcttcagt gatttcaccc atgacacgga tagcaagtcc tggtcctggg aatggttggc
     2761 gccatacgat atggtctggc ataccaagct ctgtaccaag agcacgaact tcatccttgt
     2821 aaagagtatt gagtggttca atcaattcaa actgcatatc ttctggaaga ccacccacgt
     2881 tgtggtgtga cttgatagtt tgagctgtat ccgtaccaga ctcgataaca tctgtatata
     2941 aagtaccttg agcaaggaat ttcacatctt tgagcttgct tgcttcgtca tcgaatacat
     3001 agacaaactc gttaccaatg attttacgtt tttgttcagg atcagaaacg ccagcaagtt
     3061 tgtcaaggaa acgtttagca gcgtctgctt tgacgatatt caaaccaaac ttaccaccga
     3121 gcatgtccat aacttgatca gcttcgcctt tacgaagaag accgtggtct acgaagatac
     3181 agatcaattg atcgccaatc gctttttgga gaagaacccc aacgacagat gagtcaacac
     3241 cacctgatag accaagaagg acacgtttat caccgacggt ttcacgaatt tttttgatct
     3301 gcatgtcaat gaaattatcc attgaccagt cacctttagc cttacaaatg ttaagggcaa
     3361 agttacgaag gatatcattt ccgtatacag aatgacgaac ttctgggtgg aattggatac
     3421 cgtaaatgtg tttatctggg ttttcgatgg ctgcgtatgg gcagtcagct gatgtacctg
     3481 tacgaacaaa gtcagcagga atctcagtaa ccgcatcacc atggctcatc aaaacagtct
     3541 gttcatcagg tgttgattca aaaagcgctg atggtgtgtg agttagggtt gattgaccgt
     3601 attcacgatt tccagcatca cctgcaggaa caacttttcc tccaagttta tgggtcaata
     3661 actgcatacc ataacagatt cccaaaattg gaattccgag ttcgaagatt tctgggtcaa
     3721 tatcaaatga accatcttca tatacagaat ttggaccacc tgatagaata attcctacag
     3781 gattgacttc acgaacttca gcagctgaaa ttttatggct ttttagttct gaaaaaacac
     3841 caatctcacg gatacggcgt gaaatcagct ggttgtactg gctaccatag tccaatacga
     3901 tgattttttc tacatcttgc aaatcagttg aaatgttgct catctttttc ctttctcaaa
     3961 agaaatatct tttctcaata ttctatcaca aataagggcg aattaccaac taaacaaggc
     4021 gaagtttgac tttttctagt ttattggggt acaatagaga aaagatagaa atgaagtgaa
     4081 aaatatgcta cctgcttata tgaaaatcca tgatcagata aaaaaggata ttgacgagca
     4141 ccgttgggct attggtgaga ggcttcccag tgaaagagat ttagctgagc agtttgcggt
     4201 cagtcgcatg accctccgcc aagccgtatc tctattagtc gaagaaggcg tcttagagcg
     4261 ccgtgtagga agcggcacct ttgtttccag tactcgagta caagaaaaga tgcgagggac
     4321 aaccagtttt actgaaattg tcaaatccca aggtaaagtt ccctctagcc agctcatttc
     4381 ctacaaaaaa accattccca atgagcaaga agttgccaag ctaggaattt ttccaacgga
     4441 caatattatc cgaatggagc gggtccgcta tgccgaccaa gttcccctag tttatgaagt
     4501 tgcttctatt cctgaaaaat tcattaagga ctttaaaaaa gaagaaatca ccagtcattt
     4561 cttccaaacc ttgcaaaaac atggctatcg tatcggtaaa tcccaacaga ccatctatgc
     4621 tcgccttgct aaagaaaaga ttgcccacta tttggaagtt gaaaaaggac atgctattct
     4681 tggattgacc cagatttcct acctagaaga tggtactgct tttgaatacg taaaaagtca
     4741 atatgtaggc gaacgctttg aattttatct tgaaaataat tagattacca cgcaaaaact
     4801 ctctgtcacg gacaaagagt cttttttatt tttctaagaa taaatctttc aaagaaatca
     4861 aagttacagc aaacaatatc attgccatac caagaaaatc aatgggataa aattgttcat
     4921 tcattattaa gaaggcaaag aaaatcgccg atattggctc aattgaagcc aacaagcttg
     4981 acttgaccgg tcctatcaga ctggctcctt taaggaaagc tgtataggca aagacagtcc
     5041 cgataaggat aatgcctgca aacgcaagga gaaaatcaag actagtcggg atatcggcct
     5101 gtagaacccc tgtaaaagga agggcgacca aacctgctat gaccattccc acaccaatga
     5161 ccaagctgct cccccacttt ttaatcaagg ctatgggtaa aatgatatac agagcataag
     5221 tcaaggcaga aaagagaccc cagaacagac cagcaggtgt catggataac tggtccaact
     5281 gcccatgtgt tgcgatcagg aaggttcctc cgatggcgaa tatgatggaa actatctctc
     5341 ccagtgtcgg tgccacccta tccttgatac agctataaat taaaattccg acaggacaaa
     5401 catactgaag caccgtcgct gttcccgcat tggtctcctg aatagcagac agataggcga
     5461 attggttgag aaaaagacca atcagagcaa aaataagaag agacagcaaa ctctttctat
     5521 cctttaaaaa gaccagtatt ttatcctttg cagtagcata agccaagagc atgagaattc
     5581 caccagcgat taaaagacgc aagttagtca agaccagagc cgaaattccg tgtgccatta
     5641 ggtattggcc actcgttcct gacaaccccc aagcaatacc agccacaact gttagtaaag
     5701 tcccttttaa actatttgac atgtctctcc ttactcttct ttgcgaatca agttcatcac
     5761 ttgattgcgg tctaatatcc attgagctct ctcgtccttt tcataggtac gattcgatag
     5821 gaaaatagtg gcttcttgct tctgacgatt ccacatgata aaggtacctg tatagcccgt
     5881 atggtctagc caatctcctt ccaaattcca tgctaaagaa cgttccttgt catccaaagg
     5941 agaaaaattt tgatttaagt ctcttgcaaa atcatctgct aaatagtgtt ctaaaaagat
     6001 ttgtaaatcc tttatagtcg aaaataaacc agcactccca gcatgtctac ccaggagacg
     6061 agccttggga tcatgcacta tgcctgcctc tacacctcta actgttggaa cagcaagctc
     6121 aactggccca aacttggttt ccgtcattcc ccaaggtttc cagacttgat cctttaaaat
     6181 cacatccaaa tcttgattaa aaattctttc caaaataaag cccaacagca aaaaatggac
     6241 atccgaataa agaaaggctg gctgacttcg tctgttgaga tgaaacatcg cttcctttaa
     6301 ttcaggggct gttaaaagat cacgattagg aataaaagga tcaaggtctg ttgcatgagt
     6361 caagagctgg cgaatagtga tgtctggata atcactctca ggtaaaaaat ctattaccag
     6421 tctatcaata tctaattgac ctatttccca caagaaggta caaactgtgc caaccccaac
     6481 aaccttgctg acactagcta ggtcataaac tagtcctgcc tcagtctgca agccatgctc
     6541 tgggtcactc tggcctaaat agaactctgt ccattgattg tccttaaaat acgcaaaaga
     6601 ggctccggga taaatccctg cctcgatttg ttcttctatt tttttaataa tcttggtcca
     6661 cttcatactt cttcaaacca caattcaaca ttatttctat ctttaccaag gaagaatttt
     6721 tcagacttag gaataaaata ttcagtagac tcaaacttct ggcgaagact tgctatgtct
     6781 aattcattga ccaagaactt gagcatagat aagtcccagg taaccgtatt gtccacagtc
     6841 aaatcctgcc cctgagctgg aataaaatca agggatgccc caatttcaga tgattccaag
     6901 aaactttcga tatcagttgg gagatgtaat tccatagaaa tctcaaattt acttaaagaa
     6961 attgatgcca aatctgtttg aaattcaggc ttttctccta cttctactag acttgctatg
     7021 tcatcttccg catgaatcaa aatcaaatca tcttctggtg agaaaatttc aaaagcgtag
     7081 ccattttgac ctttatataa tcgatgaatc gaatctgttt tagataagat tccttcaatt
     7141 tctaagggat tttccacctt gacaatcaat ctagctagtt tttttcttcc ctctacctta
     7201 cgagtacgca tactgggagc ttcttctaaa accagctttt caagacccgt ttggtcacct
     7261 agtgacagaa aggccgattc ttctaacaag gccttcattc caagggtttc aatataaaat
     7321 gtttcattta attttctatt attagctttt aaagtaggaa taatccgtac aatctgattt
     7381 acattcataa attcctccgt cactttttat tttaaaggat tttagaattt tttacaagat
     7441 attatgctca aatttaacat tatttagcta tttagtaaag atagtctttc aaggattttg
     7501 acaaagcctg ataaccagca atactgaggt gcagtccatc agttgtatat tcttttttga
     7561 gttggcctgc ttggtctgtc aaacaatcaa atactggcac aaattccacc tgcatatagg
     7621 cagatgcaag atcttgatag gcttgattcc agttctgaat tttttcattc gagcggatat
     7681 agactgcctg ctggtactcc tctctctcat tgacaggcaa aatggaaagc aatttaatct
     7741 ctgtcaatgg ataatcgcga gcaacggatt gaatgatagc ttcgagatta ttgagagcct
     7801 cattcacagg aacatccttt ccgatatcat ttgtcccaat cagaagaaaa attttatcta
     7861 ctgctccacc atatagatga gcatcaaggt tctctagtaa cagtcctgtc tgatagccac
     7921 gaattcctcg attgacaatc gtctttgaag tcccaaatag ctcctgtaga ggataatact
     7981 cgacaatgga atccccaata aaaagaatgt ttggttctac aacagaaatg tgatttaggt
     8041 gacgatactt agtttgaatt ttttcttgtt cctttaggag ccaattttct aataactgta
     8101 ctgccacttc attctccttt ttctaaccag ttttccaaga cttgataaac tcctccttgg
     8161 ctgttggctg gtgctagagc agtcgccaca gctttggctt tctcatcagc attttccatc
     8221 gcataggcaa ttccagccat ttcaagcatt tcaacatcat tttcactatc accaaaagcc
     8281 atgatttctt gggatttcaa gtcccagcgc ttgagtaatt cctccaagcc ccatgcttta
     8341 tgaatcccag cttggaggat atcaatgcaa ccatagccac tggatacagc tcggacacgg
     8401 ccatcaaaga gagcattgat ttcttccaaa accgaactca aacgttcctc accaacaacc
     8461 atgctcatct tgagcacacc accaaagagg tcagatgtta attcatccac aaattgcatc
     8521 cgttggtaga atttttcaat catttctgga gtcataaaac tttcaagatc tgtaaaaatc
     8581 gtaccttcct tgacaaaatc acccttcatc cccgttacaa caaactggtc ctgacacgct
     8641 cgacccttga aatgagtcaa agccttgttg acaatggcgt catcccatgt ctgagcctga
     8701 atcaattcat tgttttcaaa aatacgagcg ccattagcaa caaccagaac cactcgatcc
     8761 accaagggac tcagtagttg tctcatgcgg tgaatttcat tgcccgtcgc aatgacaaaa
     8821 cgaatgcccc tttgatccaa ctgatctaaa atcttttcca atcgtgggag atcaagctga
     8881 cctctagcat ccagcaaggt cccatccata tctgtcgcaa ttaccttaat catcatttac
     8941 tttcctctgg attcaagcta ataccacttc aatcccacat gttcattcat ttctttatag
     9001 gctgtatcaa ttcgttcctt agtcgcttca tctaactggt cacgatgact gccatcctct
     9061 ataaaatctt ccaaaatata agtttggtaa tgatacaagg gatatccttc tggtgaaaag
     9121 gttccctttg gtgttcgatt gacccaagta ggatgagctt tagctgttcc gatagttgtt
     9181 tttccatcct tcttcttgat ggtgacatcc atgagaacac cacgttcagt ccacttagca
     9241 ttctcttcat ctcccataga ttcaattcgt tgattggaaa tgaagttccc catttaatag
     9301 ataatgagtt tcttatctcc atctttttca accgtttcag atggttcaac aacgtgagga
     9361 tgccctccaa agataatatc cgctcccaaa tcgatcatct tgtgataaag agctttttgt
     9421 tcttcagttg gttccaatcg atactcaaca cccatctgaa gcatgataat ggtgatatct
     9481 gcttccttct ctgcccgttc aatttcaacc ttcatcttat cttcgtttaa atctgaaaga
     9541 taacgattat agtcttcctg agaaatatac tgctcaattc cattgaaacc ataggaatag
     9601 gctaacaatg caaccttgat accattcact tccttaatga ccagcggagc ctgatcacgt
     9661 ggttcgtgcg tataaactcc gattggagtg attccagctt tctcaataat atcggccgtt
     9721 gaaataactc cctcaatttg cgaatccaaa atatgattat gagctaaatc cagcacatga
     9781 taacctgcct ccttaatagc atccataact tcaacaggag cattaaagag aagataaccc
     9841 gctaaataat gatccttatt aatggttcct tcaaaatcac caatagctaa atctgctgct
     9901 tagtcccctt gcttgagcca aggagtcaca tactcaaaat tttcatgaaa gtcataggta
     9961 ccgtcttctt ttttagctga aaagaaaagt ccatcgtggt agagcaaatc cccgtgggcc
    10021 ataattctgg cagttttttc ctcttcctac tcctgagact tttgcttagt cccctcttga
    10081 gaaatagtat ctcgtttttg actagtcaag ggattccttg gaagctttca aacaacaaga
    10141 ccaagcccat tgataaacca actgctagca agagtatcgc cacaaatccc ttattgctcc
    10201 acttgcgata actcctaaaa agtttaccaa gcccttcata aaacgaaaag ctaaaccacc
    10261 ctgatttcga ttttgtcttc tttgtatctt cgttctccct actttcttat gcaagccttt
    10321 tctttttatt atatcacaga taagtatttc tttcacaatt gaattgaact tcccatctat
    10381 tttctataaa tcctaaatgc cataatgctt tcaattcttg tcattttgtg atatcatgta
    10441 gaggaaatga actaatccac agtggcttat tccaagtata ccacttgggc tttggcagta
    10501 gctaactgca ctaaacataa tataaggaga gaaaatgtct gcaatagaac gtattacaaa
    10561 agctgctcac ttaattgata tgaacgatat tatccgtgaa gggaatccta ctctacgcac
    10621 ggttgctgag gaagtcactt tccccctatc tgaccaggaa atcatcctag gcgaaaagat
    10681 gatgcaattc cttaaacatt cccaagatcc tgtcatggct gaaaaaatgg gactccgcgg
    10741 tggtgttgga ctggctgctc cccagttaga tatctcaaaa cgcattatcg ctgttttggt
    10801 acctaatatt gttgaagaag gcgaaactcc acaggaagcc tacgatttgg aagccattat
    10861 gtacaatcca aaaatcgtct ctcactctgt tcaagatgct gctcttggcg aaggagaagg
    10921 ttgcctgtct gttgaccgta acgtgcctgg ctatgttgtt cgccatgccc gcgttactgt
    10981 tgactacttt gacaaagatg gagaaaaaca ccgtatcaaa ctcaaaggct acaactccat
    11041 tgttgttcag catgaaattg accacattaa cggtatcatg ttttacgatc gcatcaatga
    11101 aaaagaccca tttgcagtta aagatggttt actgattctt gaataaagaa aatcccgttg
    11161 caagacgggg ttttgtgtta taatagaggc atgaaaacaa atgatattgt ctatggtgtc
    11221 cacgccgtta ccgaagctct ccttgcaaat acaggaaaca aactctacct ccaagaagat
    11281 ctccgaggta agaatgttga gaaagtcaag gaactagcta cagaaaagaa ggtgtccatt
    11341 tcttggacat caaaaaaatc tctctctgag atgactgaag gtgctgttca tcaaggtttt
    11401 gttctacgag tgtctgaatt tgcctatagc gagctagatt acatccttgc aaaaacacgc
    11461 caagaagaaa atccacttct attgattcta gatggtctaa ccgatcccca taatctgggt
    11521 tctatcttgc gaacagccga tgcgaccaat gtttcaggtg tcatcattcc caagcaccgt
    11581 gctgtcggag taactcctgt cgttgccaaa acagccacag gtgctattga acacgttcca
    11641 attgcccgag tgacaaacct cagtcaaacc ttagataaac tcaaggatga aggcttctgg
    11701 acctttggaa cggatatgaa cggtactcct tgccacaagt ggaatacaaa agggaaaatc
    11761 gccctcatca ttggaaatga aggaaaaggt atctctagca acatcaaaaa acaagtcgat
    11821 gagatgatta ccattccgat gaatggacat gttcaaagcc ttaatgccag tgttgctgcg
    11881 gccattctca tgtacgaagt tttccgaaat agactataaa aaagtttcca gtcatctgat
    11941 tggaaacttt tttatgatta actatgttct gtgataaact tgtaggcttc ttgaccagcg
    12001 atagctccat ctccaactgc tgttgttact tggcgaaggt ctttcaagcg aacatctcca
    12061 actgcaaaga taccgtcgac tgcagtcttc atgtggctat ctgtcacaat ccagcctgcc
    12121 tgatcttgga tattcaattc tttaacaaaa tcgctaagag gatccaaacc aacatagata
    12181 aagacaccac cgaaggcttg ttctgtcact tgacctgttt tcacattttc aaatacgact
    12241 gattctactc ggttttcacc cttgatttcc cttactacag aatcccagat aaagctgatt
    12301 ttttcattcg caaaggcgcg atcttgtaaa accttttggg cacgaagttg gtcacgacgg
    12361 tgaacaatgg taacagtctt agcaaaacga gtcaagaaga gggcttcttc aacagctgaa
    12421 tctccaccac caactatcaa taaatcttgg tcacggaaga aagcaccatc acacacagca
    12481 cagtaagaaa caccacgact gttcagttct tcttctccag gcactcccaa aggacggtgt
    12541 ttagaaccag ttgctacgat aactgtacgt gtttcatatg tttggtcatc agtcatcact
    12601 ttcttaaaat caccatggtc ttcgacattt tcaacataac cataaatgtg ctcaacacca
    12661 agattttcaa gtggttcaaa catcttttca gccaattcag gtccactaat attagcgtat
    12721 cctgggtaat tttcgatatc agatgtatta ttcatctgac cacctggcag accaccttca
    12781 atcaaagcta cttttagatt gcttcgagca gcatacaagg ccgcagtcat ccctgcaggt
    12841 ccagcaccga taataatagt atcgtacata tagattcctt ctttcttgtt gtaactatct
    12901 ttattctaac tctttcttgt caatcacgca aggattatgc cttgaaaaca agaattaaac
    12961 tcataaccgc aaaggataat actgggacaa ccaagactcc aatcataatc atatcataga
    13021 gatgggcttg gcgagccctg gctgtcttat caactcccga catggctctc agaccaatcc
    13081 aaatccctaa aaaaatcaga acaaggatgg tggtcaagat caaactctcg aaatataaag
    13141 aaaatagttg cagtagcatg atttctctca tttctatctt ttttaaagag taaactcagc
    13201 tagtccaact aactgagttt tcctttatct attatatcaa atataagtcc gtttgtaact
    13261 agcgaagaat tcttttgtcc gctcttcttt aggggtgtgg ataatctcat ccggagttcc
    13321 agactcgatg attttcccct tatctaagaa gagaatttta tccgcaactt gggctacaaa
    13381 gggcatgtca tgactgacca aaatcatggt ctgacctgac ttagcagcat ctgcaataga
    13441 cttttctact tcaccgacca attctgggtc aagggctgaa gttggttcgt ctaagagcaa
    13501 aacatctggt ttcatagcaa gcgcacgcgc tagggcaacc cgttgcttct gtccacctga
    13561 taaatggcga ggataatggt tttcacggtc cgaaagccca accttagcca actcttcctt
    13621 ggcaatctta gtcgcttctt ggtcagataa tttcttgaca acaaccaagc cttctttcac
    13681 attatcaagt gctgttcggc gttcaaataa attaaactgt tggaaaacca tagacaactt
    13741 acggcgtagg gcaaggattt cttcttgagt gattttagaa aaatcaactg aaaaaccatc
    13801 aatctgaata gagccactgt caggtgtttc aagataattg agactgcgaa gaaaggttga
    13861 ttttccagct cctgaagaac caatcaaggc tacaacttcc cctttttgaa tatccaagtt
    13921 cagatgatcc aagacagtct gtcctgaaaa ggatttgctt aaattcgaaa tcttaatcat
    13981 taacgaaggt ctcctttcac atctgtttgc actgtatcag gtgcagaaat agccattttt
    14041 ctctcgatga aacgaccgag gctttcaatt ccgatattga ctacccaata aacaagggca
    14101 acggagatga agcgttcaaa atagcgataa tcagctccac ctagaatctg agcttgggca
    14161 aagacttcca caacacccgc actaaaagct agagatgttc ccttggtcaa accgatgagg
    14221 gaattaatca aggttggagt agctaccacc gctgcattag gaataatcac tcgtcgataa
    14281 acttgcgctc gggtcatacc cagactgcgt gccgcctcaa tctcaccagg attaactgag
    14341 agaatggctg cacgaatggt ttcactagca taagctgcct cattaaaggc aaaggcgaca
    14401 atcgcaaaag ctgcagctgg aatcgcattg atattgagac cagttcccca ttgctgattg
    14461 agggctttca aagccaaagg aattccgtag taggtcaaca tgagttgcac caaaatcggt
    14521 gtccctttta agaaactaac aaagaaggcc tgcaagggat ataaaatctt gacacgattg
    14581 atcttcacaa tggcaaaaag aagcgccaaa accaagccaa aaagggcacc accaattgtc
    14641 aacataattg ttgttggaag ttgttggaca attctaggga ttccatcaaa gaccgaacgt
    14701 aggctaaaca gcttgccatc cggaatcaat tgcatcaagt tttggtacca atctgatgct
    14761 aaaatcgttg taacattcat aaaaacatcc tcttctgata atgattcatt ctaccatgct
    14821 tctgccgtca atgaaattat ttgctacggt cagatacaga ctttgtctac ctactagttt
    14881 atcatacttt gaaacaggga agttatatat tttatctatc aaagagatag ataaaaacta
    14941 tttcagtccc agttcttcat aagattttcg ataaccgatt tgcttaacag ttccattttc
    15001 tactaaaatg ggccgtttta acaacatacc gtcacttgct agcaactcag ccgcttcttg
    15061 gtttgacaaa cttcctacct tatcttttag ccctaattca cggtatttga taccactggt
    15121 gttgaaaaat tgcttcaatt caaatcctga ggtttctagc caattcaaaa tgacttcttg
    15181 gctaggtgtt tcttccacga tatggacggc tttatagtcc acacctaatt gatttaattc
    15241 ttgttttgct tttttacaag ttgaacattt ggggtattcg ataaattcta acatgtgctt
    15301 ctccttctat tgcttctatc tttaaaatct actccttcat gctccaagag ccaagctttc
    15361 ttttccactc ctgcagcata acctgtcaga cgcttgcctg ctcccaacac acgatgacaa
    15421 ggtactagga tagaccaagg attgcgtccc actgctccac caattgcttg agcagaagcc
    15481 acttgcaggt cttgagcaat ttgtccatag gttactgtct gaccataagg aatgccctgt
    15541 aaatagtccc aaactctctt ttcaaaattc gttccgattg gcgccaagag caagttggat
    15601 aaatcctgag gcttgccttt aaagtaatca tctaagcaag caataactgg gtctaaaata
    15661 ggatgactaa caacttcttc tatcgtttca tctcctagtc ccctctcaaa atgcttctgc
    15721 tcctgaaccc aaattccata caaataatgg tcatcagcta caagtgatag aataccaatt
    15781 ggtgaggagt agagtatttt tacatacttc ttttgcatta tttctttaat ttttgatagg
    15841 ccaagcgttc gccatctact ggcaccttac caacttgttt aaaaccaagt ttttcaaaaa
    15901 tatgttgcat aaccttgttt tcagcatgcg tatctgagcg aaaatcaaga taatcaaaac
    15961 cttcaatcaa gccctctaag aaggtttgag caactccttt tccctgcaca tctgctgcca
    16021 cagcaatacg gtgaaagact agatactctg actctccagc ttgccagttt ccctcataaa
    16081 tagcttcata ggcctcctct ggactcttgg tcacagcagc ataggctagc agttctccct
    16141 cttccaaggc tacgtaggct tgccctgaga tgatatcatc gataataaca tctgcatttg
    16201 gatagccatt ttgccactgg tcactaccag catctgctaa acattttttg gcatcctcca
    16261 tcacctgcat gatggcatct acttcatttg gaaaagctaa acgaatttcc atcttttttc
    16321 ctcatttctg actagtttct cccatcatag cataatttaa gtatttctac aagcagttaa
    16381 aacttgactt tgtgtttttg ttattttata atctagttat caaaactata taaaaaggag
    16441 ttgaaaatga aaactacctt ttcctaccca aaatgggcag aaattccaaa cattgacctc
    16501 tatctggacc aggttttgct ctatgtcaat caggtctgcg cccctatctc tcctaataaa
    16561 gacaagggcc taacagcatc tatggtcaac aattatgtga aaaatggtta cctgacaaag
    16621 cctgacaaaa aaaaatacca acgccaacag attgcccgtt tgattgctat cacaaccctc
    16681 aagtctgtat tttctattca agaaatagcc cagacactta atactctaca aactcaagca
    16741 agttcagacc aactctacga tgcttttgtg gactacatga accaaggaat tgacccagct
    16801 aaccctatta tccaaaccag ctgccaaacc attaaactct atcatcaaac tctagactta
    16861 atcgatcata ctcaagagga ggtaatccaa tgaacactag tcttaaactc agcaaacaac
    16921 tcagttttgg agaggagatt gctaatagcg tgacccatgc tgtgggtgca gtcatcatgc
    16981 ttatcttgct gcctatttca tccatctata gttatgaagc acacggattt ttatcctcta
    17041 tcggcgtttc cattttcgtc atcagtctct ttctcatgtt cctatcatcc accatttatc
    17101 actctatggc ctatggttcg acccacaaat atgttttgcg aatcattgac cattctatga
    17161 tttacgttgc cattgccggc tcatacacgc ccgttgtctt gaccttgatg aataactggt
    17221 ttggctatct gattattgtc atccaatggg gaacgaccat ctttggtatt ctctataaaa
    17281 tctttgctaa aaaggtcaat gagaaattta gccttgctct ttacctgatt atgggctggt
    17341 tggttctggc tatcattcct gccattatca gtcaaacgac acccgttttt tggagtctca
    17401 tggtaactgg cggactctgt tatacagttg gagctggatt ttatgccaag aaaaaacctt
    17461 atttccacat gatttggcat ctctttatcc tagctgcgtc cgtactccaa tacatcgcta
    17521 ttgtttatta catgtaaaaa agttgagaaa ttcaatctca acttttttct ttacacatat
    17581 tgataaagta ctggtgcaag cgcacatcat cagtcaattc tggatgaaaa gaacttacca
    17641 acatattttt ttcttgggct gcaacaattt gattgttcac tgttgctaaa atttctacac
    17701 cctcaccaac actactgata atcggaccac ggataaaggt cattggaatc ttgccaactc
    17761 ccttacattc tgcttccgtg tagaaacttc ctaattggcg cccataagca ttacgctcga
    17821 ccaccatatc catagttcct agatgactct ctttctgaga agtgatttcc ttagccagca
    17881 aaattaagcc cgcacaggtc ccaaacactg gtaagccaga tagaatggct tctcggatgg
    17941 gaagtagcat gttctggtca cgtaagagct tgcccatggt tgtagactca ccaccaggca
    18001 aaatcaaacc cgacaagtca ctctgatctt gctgaaaatc atctagattt ctgagttcta
    18061 cactctcgac acctaattga tctagcactt ttgcatgttc tgcaaaggcc ccttgcaagg
    18121 ccaatattcc gattttcatc tattttcctc gttcagccat gagaatttgg atttcatttt
    18181 cattaatacc aaccatggct tctcctaaat cttcagagat ttgagctagg atttgaggat
    18241 tacggaagtt agtcacagcc ttaacaatgg cactcgctcg tttaacagga tctcctgact
    18301 tgaaaatacc tgaaccgaca aagaccccct ctgcccctaa ttgcatcatt aacgcagcat
    18361 ctgctggcgt tgcaacacct ccagcagcga aatttacaac tggcaatttt ccatgttcat
    18421 gaacatattg gaccaattct acagggactt gcaaatcctt ggcagcaaca taaagctcgt
    18481 cctcacgtaa gttttgaatg cggcgaattt cctgattcat catacgcata tgacgaacag
    18541 cttggacgat atcccctgtc cctggttctc ctttggtacg aatcatggaa gcaccttcag
    18601 cgatacgacg caaggcttca cccaaatcct tagcaccaca gacaaaagga acttggaatt
    18661 ctttcttgtc cacatggaaa cggtcatcag ctggagatag aacttcactc tcgtcgatat
    18721 aatcaatttc aatagcctct aaaatctgag cttcaacaaa atgcccgatt ctgaccttag
    18781 ccattactgg aatactaacc gcttcttgga tttccttaat catctttggg tcgctcatgc
    18841 gggaaactcc tccagctgca cgaatatcag ccggaattcg ttccaaggcc atcacagctg
    18901 ccgcaccagc agcttctgcg atacgagcct gttcaggatt ctgcacatcc ataataacac
    18961 cacccttgag catctgtgcc aagtttttat ttagttcata acgattttca gtcatttgtt
    19021 ttatcctcat catttgtatt ggtagctacc ttttcatttt aagtcagaac agaatgaagc
    19081 acaagataaa aaaactataa ttgtatgggg acagattggc taaaaactat ctgaccttaa
    19141 cttaaggcca gatagctcat tcatttctat ttttcagccg taagggcagc cattgtgatg
    19201 tagttgtatg gtttgttgaa gtgtggcaag aagaagaggt ctgtcaatgc caatttatca
    19261 attgtcacat gctcttggat agcaagtgag aacatgtgga ttcccatgct aattgcaata
    19321 tcatgtgaaa ccatttgggc accaagaatt tcacggctat ctttgtcaaa gacaatctta
    19381 attgctactt catggttgtc atgtttcatg aattctggtt tttgaagatc gttaaagcct
    19441 gtttcagttg cgttgtaacc agcagctttc gctttttcaa gagtcaaacc agttgaaacc
    19501 atgtgaagac cgtagattga gataccattt gaaccttgaa caccgattcc ttccaattca
    19561 tgtccacaag cattgtaggc accaacgata ccagtgcgaa cagcatttga agcaagagcg
    19621 atatagcttg tatctttacg agcattgtca taaacagtcg cacagtcacc aacagcgtaa
    19681 acgcctggga tagatgtttc ttgtttcttg tctacaagga aggcaccgtt gcggaagagt
    19741 tcgatcttac catctgcaag ggctgtgttt ggacggaaac caactgcaag gataaccata
    19801 tccacgtcaa agctttcttt gtcagtaatc aagcgttcaa ctttaccgtc accttcgatt
    19861 gctttaacag tttgacctag agccaagcgg atgttgtgat cttccaagtt cttcgccatc
    19921 atttgtgtga agtctttgtc atagtaaccg ttcaagacag tatcaacgat atcaacaagg
    19981 acaacttctt ttccaagacg ttcaaaggct tcagcaagtt caacaccgat gtaaccacca
    20041 ccaacaacgg cgatacggtc gaggtgttgg ctcttgtcag aaagtttatt gataacttct
    20101 tcagcatttt ggtacaattt cacgaattgt acgttttcaa gagttgcttt aaattcgcgg
    20161 tttcctttaa caatttcaac accttcgatt ggtggcaaga ttggtgtaga acctgtagcg
    20221 aaaatcaatt tttcgtatga ttctttgtgc tcttttcctt caacttccgc tgtaattact
    20281 ttgttatcat agtcgattga aagaacaggt gagttcatgt aaactttagc acctttagct
    20341 tccaattttt ctttatcaga atagaacaag ccttcagcac cgtcaatttg ttcaccaatc
    20401 caaagagcca ttccacatcc taggaaagag atgttagagt tttggtcaaa tacaacaatt
    20461 tcgttctcat ttccaaaatt atccaacatg gtattgatac atgctgtacc agcgtggtta
    20521 gcaccgacta caacgatttt actcataaaa atttcctgcc ttcattctaa atttacctta
    20581 ctagtatacc atttaccgtt agcgtttaca aggggggtac tattatttca gcatttttta
    20641 tcaaaaataa ttccccattt atcctgtttt caagcctgat gaattttatg agcatatttc
    20701 ttgacttttt ccaaattttg tttatgaaaa ttctaacttt atggtattat aataacatga
    20761 aaatgaaatg taagggcttc aatttagatg attatttgat attacaactt attttctata
    20821 agagaaagga gttggtagct tgactcttcg ttcacattct gaacggctaa tggggactac
    20881 tatcactatt tcattagtgg atgaacaagc cgatatcttt ctccaaaaat cctttgactt
    20941 gctcaaagaa cttgaatacc gcttcaacgc caatagtcaa gaatctgagt tgatggaaat
    21001 caattatcaa gcaggaatag cccccgtcac ggttcatcca gacctgtttg agctgatttc
    21061 acttgggtta gagcatagcc tagcgctctc tagccacctc aatatcagca ttggtccctt
    21121 aattcaaacc tggcgtatcg gtttttcaga tgccaaggtc gcccagcctc aagaaattga
    21181 atcggtgctg cctctaatca atcctcatgg tatcgagtta gattcttcta cttctactgt
    21241 gtttttaaaa cagaaaggaa tgaagatcga tctaggttgt ttagccaaag gatacagtgc
    21301 ggataaggtt gcccaatttc ttaggaaaga gggggtgact tctgccttga tcaatctggg
    21361 agggaatatc ctgaccattg gaaaaaatca ggcaagaggg gataacccat ggcaaatcgg
    21421 gattcaagac ccagccaatc ctaggggaaa tcatttaatg accatccctg ttgtcaataa
    21481 atctgtcgtg acttcaggca tttatgaacg tcacctgacc gtcgatggac aagattacca
    21541 tcacattttt gacagtcaaa caggatatcc tgttgaaacg gaactagcga gtctaacaat
    21601 catctctgat aaatcagtcg atggcgaaat ctggacgact cgactctttg gagaaagacc
    21661 tgcttctatc ctctggcaag tcgaaagttt ggagggcatc gaagtcatcc tcatcgataa
    21721 agaaggccac ctaagctgtt cttcaggaat tcctactcta tagagacaac tgtttcaatg
    21781 gaattttaaa attgtattat gtaaaaagag aaaggaaatc tcatgttaaa acttattgct
    21841 attgttggaa caaattcaaa acgttctaca aaccgtcaat tgcttcaata catgcaaaaa
    21901 cactttgctg acaaagctga aattgaactt gttgaaatca agaccattcc tgtcttcaac
    21961 aaaccagctg acaagcaagt acctgctgaa atattggaaa ttgctgctaa aatcgaagag
    22021 gcagatggcg ttattatcgg tactcctgag tatgatcact ctattccagc tgttttgatg
    22081 agcgctcttg cttggttgtc ttatggtatt tacccacttt tgaacaaacc aatcatgatt
    22141 acaggtgctt cttacggtac gcttggttca tctcgtgccc aattgcaact tcgtcaaatc
    22201 ttgaatgctc ctgaaatcaa ggcaaatgtt cttccagatg aattcttgct ctcacactct
    22261 cttcaagcat ttaacccaag tggcgacttg gttgaccttg atgttatcaa gaaattggat
    22321 gccatctttg atgacttccg tatcttcgtg aagattactg agaaattgcg caatgcgcaa
    22381 gaattgcttc gcaaagatgc tgaagacttt gactgggaaa atttgtaaga taggagaccg
    22441 aaagaatgaa atttgttgga cttgttggat caaactacga tcaatcatat aaccgcaaac
    22501 tcttggaatt tatccgtcgc aatttcaaat tcaaatttga attagaagta cttgaaatcg
    22561 acgaagttcc aatgtttaac caagatgaaa aatgggacga aagcttccaa ttgcgtttct
    22621 tgtataacaa gattacacgt gctgatggtg tcattatcgc cactcctgag cacaaccaca
    22681 ctatctcagc ttcactcaaa tctgtacttg aatggctttc atacgaagtt catccatttg
    22741 aaaacaagcc tgttatgatt gtgggtgcat catactatga ccaaggaaca tcacgtgctc
    22801 aagttcacct tcgtaaaatc cttgatgctc caggtgttaa tgcctacacg cttccaggta
    22861 acgaattcct tcttggtaaa gctaaggaag cttttgataa ccacggaaat atcaccaacg
    22921 aaggcactgt taaattcctt gaaacttgct tagataactt tgttaaatac gtaggagtcg
    22981 tttcgaaatt gaaaaaacca aaaccaatcg aatcagaaga cttggattgt ggaaaaccaa
    23041 tcgctacaac catcacagaa gttgaccctg acgatccaga atgggtagaa aaagttgcag
    23101 caatcactgg tgctgtttct ggtgatacct atgtcaaatt ggaccacggt atcctaacag
    23161 ttaaccaaat tgatatgttc ttgaaagcta tgccatttga attgacatac gctgacgaca
    23221 acaaccaatt cctctactac aacaacgctc accaagatcc agacactatg ttcgctaaac
    23281 gtgtaccacc tcaatcaggt agccgtatgt cgactgttca tggttctctt ccaccagcac
    23341 gtatgaagaa cgtagagtgg gttatcggaa cacttcgcaa cggaaaccaa gaatacgtcc
    23401 gtacgatcgt tccaggttct cctgcaggtg ttatcaacac tcacaactac caagcaatgt
    23461 actatcctga tggatcatac gctggtatca atgaaatcgt ctttaacttc caaccatggc
    23521 ttgactggta cctaaaagag actggtcaac gtttggtggg tggtagcgga ccatttgctc
    23581 ctgctgccgg agatcatgga gacgccgatg ctacttctgg cgcttctgat tcaggggatg
    23641 ctggaggcca cggtggtgac gcagacgcta cagccggtgc aagttactaa tgatagtttt
    23701 taggaaccat tatggttcct tttttagcga aaaaagacta acaaacacaa actgctagtc
    23761 tctttgttat cgtttcatct atccttgtta aaagaaagaa tacttatact caatgaaaat
    23821 caaagagcaa actaggaagc tagccgcagg ctgtacttaa gtacggcaag gtgaagctga
    23881 cgtggtttga atttgatttt cgaagagtat tattatgaat ttgggtcatc aagactacgg
    23941 ccatgtaatc ctttttcacg ttgtacttgg cgtagttttt ctggtgtaac atcgtttcct
    24001 tcttcgtcca caattttgat tccttcaatg tggtgacgaa cagcgcggcg ataaccttcg
    24061 atgtactcct cacgtagttt ggcttgttcc actttttctt ctggtgttaa gccttctgtt
    24121 tttttctttt tagcaagctc attgatacga gcaatttttt tcggatccat ttcttctcct
    24181 ttgtaataag ataaagtccg tttatcaaat tttatttagt gttaatttgg ttaaaacaag
    24241 caaacttagc tgctttcttg gttagttgtg acaagattgc caaacgattt tgacggacag
    24301 cctgatcttc agccattacc atagtatttt caaagaaagc atcaatgact gggctaagcg
    24361 caaagagttg tttcaattgc tgacttgcag gtcctgataa aacgagtgtt tctactgctt
    24421 ctgccaaagc tttttcttgg tcattctcaa atagtgctga atcaactgta gcaacccctt
    24481 ctgccttctc agccaggtta aaggcacgag aaagtgattc aacagatggt ttaaaatctt
    24541 cttccttgct tacttctacg agagcacttg ctgcttccaa catatctgcc acaacaaagt
    24601 ttgaacctgc aagaactgct tccttgatat cttttggagt agagcccatc atcttatcaa
    24661 cacgagcctt gataaagtcc ataacctctg ctttattttc ataagtcaaa ctgtcaaatt
    24721 tcaatgcata aaggctatca atcagctcat ccatagcaat gtgccaacca aaggcatcca
    24781 agatacgaac cacaccttga gttgcacgac gaagggcata agggtcatta gaacctgatg
    24841 gaatcaatcc tactgagaag aaactcaaaa tcgtatccaa tttgtctgca atggctagaa
    24901 ctgcgccgac cttgctctct ggaagttctc cttcagctga tgtaggcatg tagtgttcac
    24961 gaatagcagc tgccaccgct ggagtttcac cagcaagaag ggtgtatttt tcacccataa
    25021 ttccttggag ttcgtcaaat tcaccaacca tacctgtcaa caagtcaaac ttgtaaatgg
    25081 ctgctgcacg agcaaggtca actgtttcat ccactgacaa accagctttt tctgccaaaa
    25141 gtacagtgat ttgacccgta cgaatcatgt gttcacgaag ggaaccaatc ttctcatgga
    25201 aggtgacatt gtttaatttt tcaacaagat ctgaaatcac caatttttgg tcttcacgcc
    25261 agaagaattc tccgtcttcc aagcgagcta ccaagacttt ttcatttcct ttaatgacat
    25321 ttttcaaacg ctctgcgttt ccgttacgaa cagaaatgaa gtttggcaag agttttccat
    25381 cttgatcacg aacaacaaag taacgctggt gttccttcat agaagtcacc aagacttctt
    25441 ctggaacttc aaggtattta gcatcaaaac ttcccatgaa ggcagttggg tattcaacca
    25501 aattcaagac ttcattcagc aaatccgcat caatttcgat acgtacacca tgttttgcct
    25561 caatttcctt gatttggtca acaatcattt gctcacgttc acatggatct gcgattacaa
    25621 actgcttacg aaggtcttct tcatagctca atgctgactg aattttggtt tctttcccca
    25681 agaaacgatg accacgactc acacgacctc ccttgatatc aaggaaatcc aaatcaaact
    25741 cttcttcatc taaaagaaca gttaaagtgt gaacaggacg aatgtattca aagctatttc
    25801 cagcccagtg catgctgaca ggaaaagtca gtgacttcaa gacatctaca acaccaggaa
    25861 caatggcttc aactgattgg ccaacttctt ccttagtgac atagacatat tcttcaccct
    25921 taatttcacg gaattcgata tcttcaacag tcaaaccttt cccacggaca aatccttgag
    25981 ctgctttggt gaagtttcca tcactatcca aggcaatttt ctttgctggt cccttgaaat
    26041 cttctgtcaa atcagactgt ttgtctgata aaccagtcac acgaacagcc aaacgacgtg
    26101 gtgttgagaa ggtttgaatg gcttcaaaag acaggcggtt ttccttgagg aaggctgcca
    26161 ttttttcgcc tagttgtttt tcacttggtg tgacaacata ggctggtaat tcttcaagac
    26221 cgagttctac taataagttt tttgtcatgt tttttccttt acattttctt caatcttttt
    26281 tgatatgcac cttaggtact ggggacgtcg ctaactaact ctgtgagtct gtatggaaat
    26341 ccaatgctaa atttatcaag atttccaaca atctcataaa agtggattta gctgactgat
    26401 ttttgaacct aaggtttcaa aaatccccac ataacagtac ggcggtgcat atccctctag
    26461 caagtcttcg acttgcctct aacgatttta gagtacagta ttactcacta ttctgcgtct
    26521 tctgctaaaa gtttaactcg tgtttcttca tccaaaagtg ggtaacctag gcgtttgcgt
    26581 tctgcaacaa aggttttggc tacaacacgg gccaagttac ggatacgagc gatatagcct
    26641 gcacgctctg ttacagatac ggcaccacgc gcgtcaagca gattaaaggt atgtgaacat
    26701 ttgagaacat agtcataggc agggtgtacc aagccttctt ctaatgcacg accagcttct
    26761 ttttcaaact tatcaaagtt ttcaagcaac atttcttggt ccgaaatttc aaatgaatat
    26821 tttgagtgct catactcagg ctggataaag atttctccgt attttacacc atcagcccac
    26881 tcgatatcat agacagagtc tacttcttga atgtaagaag ccaagcgctc caaaccatag
    26941 gtaacttccg cagtcacagg gccagttgcc aatccaccga cttgttggaa ataagtgaac
    27001 tgagtgattt ccattccgtc aagccaaact tcccaaccaa gaccagctga accagttgat
    27061 gggttttccc agttgtcctc aacaaaacga atatcgtgct ccaaaggatt gattcccaat
    27121 ttttccaaag actcaaggta aagttcttgg atatttgatg gagaaggctt catgaccacc
    27181 tggaattggt ggtgttggta gagacggtta gggttttccc cataacgacc gtcagcagga
    27241 cgacgtgatg gctctacata agctgcattc catggctcag gtccgatagc acgaaggaaa
    27301 gtgtaaggac tcattgttcc cgcacctttt tcattgtcat aagcctgcat aagcatacaa
    27361 ccttggtcat tccaaaatcg ttgcaaagtc aaaataattt cttgaaatgt taatttctta
    27421 gacattgaat actccttaat aaaaaataat ttcttgcgac acgagtctac ctcgtcgatt
    27481 atacgaggca agacgagtta gtactgcgtc tagctcagca ccctagtgct gagcgtgggg
    27541 cagtccgact gtaaggaggt ctctgccact gatgcagtga aatgtcaatt tcttagacat
    27601 tgtttactcc tttagttttt atatttcttt ttcaattaat ttctgctgaa caaaccaatc
    27661 taacaaggga aaagtttttt ctgtatttgc ccataaatga taagaatcta agaccgcttg
    27721 tacactacct aaattttcta ccaacttatc tactgtcaaa aagctgcgcc agtattcata
    27781 aaaaatactt gcatagttct tatcataagt cgaagaacca aaatcattca atgaatgcca
    27841 accatacttc ttctgaaaaa gttctactag agattgatta caaatttttt ccgcttgaaa
    27901 ttcctcttct gtcaagaaat acttgcgact aatatattca accatcccct cttcgaacca
    27961 gatataagag tcataaccat caaaatcatc taaaaaatgc tccgaccaat gagctaactc
    28021 atgtcctaca atctgtaaga ggaaattttc agataaagaa tgatagtgac tttctattgc
    28081 ttgagtttgc tgagaacact cgtagtccat caattgatac aaatacaact ctttccaaac
    28141 agctaaatca ggcgtcataa ccattcgtct attatttgta taggctggaa cagcgctttc
    28201 cctaataatc tgtgtagcag cattaaaatt tgaccaaata acggcttgcg gtaaatcaca
    28261 gactgcaagt tcattcttta aaaaagattg ataatctttc aacttttcag tatttcttac
    28321 tacaaaatca cgaaatgcag ctagttgact atcgtctttt acaagataaa ggttttccac
    28381 gtctctcctt tctttcacac aatagactga ataagcaaaa gaaccacatc tgacaatcct
    28441 aggttgattt aacctagggg cgtcagaaac gcggttccac cctaatttat tacttcattg
    28501 tttttgaaaa tactacagaa agcgccagct cattattttg ccctacttgg ctcccactgt
    28561 cccaagctcg cttgaagaac gccataagac tttctttcct gctgactatt atatcaaaaa
    28621 ttagaataat gtacaattct ttttatgatt ttctagaaat ccacatcatc aactcgtgga
    28681 gcacccgatt gaacagcaat cgtttttaag gtttctctct cctcatgact cagttcaatt
    28741 ccaaaacaat caagattagc ttgaatacga gaggttgtga cagattttgg aagtggtaaa
    28801 aatccttctg ccaagctcca ggccaaggct atctgagcaa ccgattttcc gtgatttgct
    28861 gctatttctt ggacttgctt gctatcaaac agttctcctt gtccaaaagg cccccaagct
    28921 tccaataaaa ttcccttttc acgacagtaa gctacgactt gatcttgata cacacctggc
    28981 gccaagcgaa cttgattgac cgcaggaacg atagttgcag tttcaagcaa ggcatccaaa
    29041 tgatggggaa gaaaattgct aacgccgata gcacggattt tcccttcttg atagaggtct
    29101 tccatcgctc tccaaacttc cgcattgcga gttttccatg cgtcattttc tctgagcggt
    29161 tttgggttcg gccaatgaat caaatacaaa tccaaataat ccaagcccag tttttctata
    29221 gatttttcca aagcttgacg agtttgctca taggtttgct gactattcca aagcttggta
    29281 gttacgaaca tttcttcacg tggaactccg ctatctttga ttgcttgacc aacactttct
    29341 tcattctgat aaatcgccgc cgtatcaata tgacgataac cagccttcaa ggcttctaac
    29401 actgcacgat aggcttcttc tccatcctta gccttaaaag ttccaaatcc caatactgga
    29461 atttctactc cattatttaa ttgataggta ttcatatgct tctccttcta ttttattcat
    29521 caattatagc ataaaacatt ttttaagtag tactatcttc tgtaaaaaat aagtctgtca
    29581 aatatctaac agacctattt tttcttgctt attcatcacg actatagtac agctcatgag
    29641 cttttaggcg agtattgagc atctctggta tggtcacaaa ggtataacct tgatttttca
    29701 aatactcaat gacccttggc aaggcattga ctgtcggact gtgaatatca tgcatcaaaa
    29761 cgatagagcc gttagctact tgatgctgaa tttctgtcaa aatagatgct tcatttttac
    29821 tcttccagtc cagactatcc acatcccaca tgataaagct caaatccaag ctattgcgaa
    29881 tatcatctgt aatagcacca taaggtggac gcatgagttt agaactagaa cccagcactt
    29941 tagttagcac atcctcagta tcagtaatct gctttttagc ttcatcaaga gagagttgcg
    30001 agagaatcgg atggctccaa ctatggtttc caacaacatg accttcagat tttatcctct
    30061 tcaccaagtc ctcattccca gaaacatttt tcccaagcac aaagaaagtc gctttaatat
    30121 catatttagc tagggtctct aatacctgcg gggtcgttgc tggatttgga ccatcatcaa
    30181 aggttagagc gacaactttt tgatgtttct tatcaaagta agattggtac aaggccgcat
    30241 ctttttcgag taagtacgaa gattggataa catcaaagaa agcagatact ggcaaggcta
    30301 tctcttctaa attttcaacc acaggacttg gataaaggat aatctgacta tccttgtaat
    30361 caaaattcca tgcagacaag tcttggtcag agaagttttt tacaatctgc tcactctggt
    30421 cttgctctat ttttttatcc tctatgaagg aggtcaactc ttttatcagc tgttccttag
    30481 ccttactagc atctgaaaac agttggtcaa gtgtaaaagg ttgcccatct tctgttaagt
    30541 gaacctttcc tagactggtc ttttcagtct cttcaatctt aaaagcaact aaatcataga
    30601 tttgtttggt cacattacga ttaacgactc cctttaaacc tgactcttct tgctctgtat
    30661 agtagaaaac aagattgtcc ttactttcta gcttgtcctt aacatcttga tttatcagct
    30721 ccctaacaga ggaaatcact ttctcccctt ggagaggata ataggcaatc acttcggctt
    30781 gcccctgacg aaaatgctcc ttctgatttc cctcactcaa ttgatcatct ttctcttttt
    30841 tgagcgattc aatcttttgc tcaaaacttt tttgctggta gatcttaaaa gcaataaatc
    30901 cgcctaatag gcaaatagaa attgctaaaa taccaatcaa agccaataat acatgtctcg
    30961 ttttcccgtg tctgccacgt cctagtctac ttttattcat atttatatca taacaaattt
    31021 gagcaataat ttcaatgata caatttggca atccacaatt cctctagcaa gccaaagcat
    31081 cttacatcaa ctccaacatc cgtatctgta gagattatgt agttttctat ctatggaatt
    31141 tctagtagat tgaagctaga atagtagacc tatacttcta aaacattgtt agaaatcgat
    31201 ttgactgtcc tgatcgattt gtcctgttct catttcattt cactataaaa ctatgaaaag
    31261 ctaggtcatc tgagaagtaa ttactatcga caattgcgat agcgtatact agtgcgattt
    31321 gtttgtagct ctggtgggta tcaccttcac gttggcgaat tttttctagg ttttgattaa
    31381 cctgaatgca cagataagcc cataggcgat tgatgaaaaa attctgatga tgaacctgaa
    31441 tttcgatgat aacttggatt ccattatcta actccgccaa gacgtctata ctagtataga
    31501 aatcttgcgc tgagtagggc atggaaggca agacgtgaat gttacttccc tccaaaatcg
    31561 tcacattttt tgctggcaag tccagcatat cgcggataaa ttgacaaatc attagttggg
    31621 ctgatacctg gatgtctgag aatcctcctt ttcctccttc tattatagca catttttaaa
    31681 tctaggtttg ctagattcta tgcttctatc tatttattcg gaaaagaaag ctaatagctt
    31741 atttgaaatt ttctcctctc tgttggatag tcccgtctat ctatgttata atgaaagaag
    31801 gatttaaaat cggaaaagga gtatttatgc taaaattagg tgtcatcgga actggcgcta
    31861 tcagccatca tttcatagaa gcagcccata ccagtggaga ataccaactg gtcgcaatct
    31921 attctagaaa actagaaact gcagcaacct ttgcttctcg ctatcagaat atccaactat
    31981 tcgatcaatt agaagtcttc ttcaaatctt cttttgattt agtctatatc gccagtccaa
    32041 actccttaca ttttgctcag gcaaaagctg ccttgtctgc tggtaaacat gttattctcg
    32101 aaaagccagc tgtcactcaa ccacaagaat ggtttgattt gattcaaaca gctgaaaaaa
    32161 ataactgttt tatctttgaa gcagctcgta attaccacga gaaagccttt actactatca
    32221 aaaacttttt agcagataag caagtgttag gagcagattt caactatgcc aaatactctt
    32281 ccaagatgcc tgacttgttg gctggacaaa caccaaatgt cttttcagac cgttttgctg
    32341 gtggagccct catggatttg gggatttatc ccctctatgc tgctgttcgt ctttttgaaa
    32401 aagctaatga cgcgacttac catgctcaac agcttgacaa tagcattgac ctaaatggag
    32461 atggtatcct cttctaccca gactatcaag ttcacatcaa ggctggaaaa aacatcactt
    32521 ccaatcttcc ttgcgagatt tatacaacag atggaacctt gactctcaac acgattgagc
    32581 atattcgctc agctattttt accgaccacc aaggaaatca agtccagctt cctatccaac
    32641 aggctcctca tacgatgact gaggaagtcg ctgcatttgc acacatgatt cagcaaccag
    32701 atctgaatct ctaccaaact tggctgtatg atgcaggctc tgttcatgag ctactatata
    32761 ccatgcgcca gactgctggc attagatttg aggcagaaaa atgaaaacca aactaccgac
    32821 tgaatggcaa gaactgagtg accaactcgg tttccaagaa ttcaccccca ttcaaactca
    32881 actatttgag ccccttcttg ctggagaaaa cctcctagga gtgagccaaa ccggaactgg
    32941 taagacccta gcgtatctcc taccaagtct tctcagacta caaaagaaaa aagcccaaca
    33001 actcttgatt ctagcaccaa acacggaact tgctggacag atttttgatg tatgtaaaac
    33061 gtgggcagaa gctatcggat tgactgctca actcttcctt tcaggttcaa gtcagaaacg
    33121 acaaattgaa cgtcttaaaa aggggccaga aattctgatt ggaactcctg gccgtatctt
    33181 tgaactcatt aaacttaaaa aaatcaagat gatgaatgtg gaaaccatca tcctggatga
    33241 atttgaccaa ttactagatg attctcagat tcactttgta gaaaaaatca ctcactacgc
    33301 acctcgtgac caccaactgg tctacatgag tgccacgacc aagtttgacc aagaaaagat
    33361 tgtgccgaat acacgcacta ttaatctctc tgaccaaaaa ttggacaata tccagcattt
    33421 ctacatgcag gtagaccaac gtcatcgagt ggatatgcta cgaaaactgg ctcatgtaga
    33481 ggatttccgt ggtctagtct tcttcaatag cctgtcagac cttgggaatg ccgaggaaaa
    33541 actacagtat cgtgatatat tgactgtttc cctcgctagt gatgttaatg ttaaatttag
    33601 aaaaatcatc ttagaaaagt ttaaagataa ccaactaacc ctgcttcttg caactgacct
    33661 tttggctcgt ggaattgata tcgatagcct agaatgtgtc gtaaactttg atattcctag
    33721 agatagcgaa acctacacac accgtgctgg ccgtactggc cgtatgggca aggaaggata
    33781 tgttattact ctcgtcactc atccagaaga aatcaaaaaa cttaaaaagt ttgcaagtat
    33841 ccgagaaatt gtcctaaaaa accaagaact ctatatcaaa taaagcccca ttgctctata
    33901 cacatccagt gtatggagct tttttataag caattcaatt ttatactcat ctgctttcaa
    33961 aaagcattct attccatctc cgattaacga tggactttat cacctccttc tccagtcctt
    34021 gtatgacatc ttggagttga ttcatgacat cttccaaagt tcgaaaggct ttattcttaa
    34081 atccacgttt acgaatctct ttccacactt gttcaatggg ttcatctctg gtgtgtatgg
    34141 aggaataaag gtaaaatcaa tattagtcgg aatctttaag gtacttgatt tatgccatat
    34201 agcattgtcc ataacgagta aaaaataatc atctggataa gcttgtgaaa gctcttctaa
    34261 aaagacgttc atccactcag tattacatcc accagctatt aagaaaaatg attcgcctgt
    34321 atggggcatc aacagctcca taacaatagc gaaattctcg tatatagtga ctatggacat
    34381 gtggacctac tcctattgga gaccaacaag atcccagttt actgattcta ccgaaaccag
    34441 cctcatcttg gtacatcaag cgaaccttat gaaaaggtct actggtttta aagcgctttc
    34501 ttgtcttctt gaattgagat tttattttta gacgcgacaa tggtttgagc gtctgctttc
    34561 ctaggatgtt ctggacgtgg cataatattt cgccaaccat ggtgcttcaa cagttgatag
    34621 aaggcatcac gtgtgtagga acgatataag cctgaaataa ggtattgtaa caaattctcc
    34681 tgcctctgta gccttcaaat ggcgggcaag aaaggctttc tcttcctcaa ctgtcatata
    34741 tgcatggtta cgaccaccac gtgtttcttg aaggagagag tcgagtccga actcctcata
    34801 tttttttaca tttcgccaaa tcgttgtttg attacagtct aaaagatcta taatctcttt
    34861 ataagatttg cccatcagac gaaatagaac gatttgaagg cgtttatgat atttagctgt
    34921 acgaaagtct tttaaaagtg ttttgatggt ttggatttct tctttagttg atttcatatt
    34981 actattgtat aatgcttttt gatttgagtc tagtattata ctcttcgaaa atctcttcaa
    35041 accgcgtcag cttcgccttg tcgtatagat gttactgact tcgtcagttc tatctacaac
    35101 ctaaaaacag tgttttgagc agtctgcagc taactttcta taacaaaatg aaataagaac
    35161 aagacaaatc aatcaagaca atcaaatcga tttctaacaa tgttttagaa gtagatgtgt
    35221 actattctag tttcaa
//
LOCUS       DAVKQL010000017        34631 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVKQL010000017 DAVKQL010000000
VERSION     DAVKQL010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34631
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.017"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            787..1239
                     /locus_tag="VW561_000753"
     CDS             787..1239
                     /locus_tag="VW561_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9943040.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            1258..2595
                     /locus_tag="VW561_000754"
     CDS             1258..2595
                     /locus_tag="VW561_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEU9943041.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIRGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2674..3558
                     /locus_tag="VW561_000755"
     CDS             2674..3558
                     /locus_tag="VW561_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9943042.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3558..4391
                     /locus_tag="VW561_000756"
     CDS             3558..4391
                     /locus_tag="VW561_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9943043.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAVFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4409..6502
                     /gene="nanB"
                     /locus_tag="VW561_000757"
     CDS             4409..6502
                     /gene="nanB"
                     /locus_tag="VW561_000757"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEU9943044.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISSLLLDKLSGESQTVVMKFKADEPNSLQALFGLSNSKADFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLREKHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSEVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6514..7617
                     /locus_tag="VW561_000758"
     CDS             6514..7617
                     /locus_tag="VW561_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU9943045.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDVCKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVRLSEIVK"
     gene            7791..8489
                     /locus_tag="VW561_000759"
     CDS             7791..8489
                     /locus_tag="VW561_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEU9943046.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8508..10025
                     /locus_tag="VW561_000760"
     CDS             8508..10025
                     /locus_tag="VW561_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9943047.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADPAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPFGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            10150..11478
                     /locus_tag="VW561_000761"
     CDS             10150..11478
                     /locus_tag="VW561_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9943048.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11730..12596
                     /locus_tag="VW561_000762"
     CDS             11730..12596
                     /locus_tag="VW561_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9943049.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            12612..13451
                     /locus_tag="VW561_000763"
     CDS             12612..13451
                     /locus_tag="VW561_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9943050.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13724..14176
                     /locus_tag="VW561_000764"
     CDS             13724..14176
                     /locus_tag="VW561_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9943051.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNQFEHHKNYADLHLLIEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            14273..14617
                     /locus_tag="VW561_000765"
     CDS             14273..14617
                     /locus_tag="VW561_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943052.1"
                     /translation="MVSSKYAARASFLDGQGITVDEMSWIINGIVNGLIGRYIKLGIY
                     AAKYGISMVRSILSRAAATAAARVGLLTKIFGWILRIAVLAADVYGNFANNIAVAWDA
                     HDKIPNNGRINF"
     gene            14897..15541
                     /locus_tag="VW561_000766"
     CDS             14897..15541
                     /locus_tag="VW561_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU9943053.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            15710..16627
                     /locus_tag="VW561_000767"
     CDS             15710..16627
                     /locus_tag="VW561_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU9943054.1"
                     /translation="MSDLKKYEGVIPAFYACYDDQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGARAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEVLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            16645..17529
                     /locus_tag="VW561_000768"
     CDS             16645..17529
                     /locus_tag="VW561_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9943055.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17545..18396)
                     /locus_tag="VW561_000769"
     CDS             complement(17545..18396)
                     /locus_tag="VW561_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEU9943056.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18596..20653
                     /gene="pbp2b"
                     /locus_tag="VW561_000770"
     CDS             18596..20653
                     /gene="pbp2b"
                     /locus_tag="VW561_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEU9943057.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            20664..21260
                     /gene="recR"
                     /locus_tag="VW561_000771"
     CDS             20664..21260
                     /gene="recR"
                     /locus_tag="VW561_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEU9943058.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21436..22479
                     /locus_tag="VW561_000772"
     CDS             21436..22479
                     /locus_tag="VW561_000772"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEU9943059.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            22563..23936
                     /locus_tag="VW561_000773"
     CDS             22563..23936
                     /locus_tag="VW561_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEU9943060.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIKTAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIHQAFQGLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSIQLHNQMILSLSPDVLDTVIFYGEDIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLAKQVKESLGAYDQILLKGSNSMNLAMLVE
                     SLENETK"
     gene            23923..24534
                     /locus_tag="VW561_000774"
     CDS             23923..24534
                     /locus_tag="VW561_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9943061.1"
                     /translation="MKPSDFVKYLQRMIALTDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQGSDLDAEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIEILWQVYQGQREQYLD"
     gene            24625..25329
                     /locus_tag="VW561_000775"
     CDS             24625..25329
                     /locus_tag="VW561_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEU9943062.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            25548..26921
                     /gene="ftsA"
                     /locus_tag="VW561_000776"
     CDS             25548..26921
                     /gene="ftsA"
                     /locus_tag="VW561_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEU9943063.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDTAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26938..28197
                     /gene="ftsZ"
                     /locus_tag="VW561_000777"
     CDS             26938..28197
                     /gene="ftsZ"
                     /locus_tag="VW561_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU9943064.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETAELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            28202..28873
                     /locus_tag="VW561_000778"
     CDS             28202..28873
                     /locus_tag="VW561_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU9943065.1"
                     /translation="MNVKENTELVFREVAEASLSANRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELAGLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNIPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28883..29422
                     /locus_tag="VW561_000779"
     CDS             28883..29422
                     /locus_tag="VW561_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEU9943066.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTSVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29422..29685
                     /locus_tag="VW561_000780"
     CDS             29422..29685
                     /locus_tag="VW561_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEU9943067.1"
                     /translation="MIFLIRMIYNAVDIYSLILIAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            29682..30467
                     /locus_tag="VW561_000781"
     CDS             29682..30467
                     /locus_tag="VW561_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEU9943068.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            30476..31264
                     /gene="divIVA"
                     /locus_tag="VW561_000782"
     CDS             30476..31264
                     /gene="divIVA"
                     /locus_tag="VW561_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEU9943069.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMAELQARIEV
                     ADKELSEFEAQIKQEVEAPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            31612..34404
                     /locus_tag="VW561_000783"
     CDS             31612..34404
                     /locus_tag="VW561_000783"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEU9943070.1"
                     /translation="MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFETEDFVQLSELPEAQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SDLTIAEGPAPEAALSFEDVAFTVERAAGEVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFADAVAEGFEEK"
BASE COUNT        10673 a         6106 c         7503 g        10349 t
ORIGIN      
        1 ctgttataga ttcaatatat tatgtgacta ttaaccttct cttctcctaa aattgacttt
       61 cttgttttct tatcttgtcc actcgaaaca agtattgtaa gaatttgatt atttttgaaa
      121 gtacttttaa tatacttgat atagttaaaa aagatttgaa actaaattcc aaattagaaa
      181 aagacttgaa atactaaaaa aaaaagtata ctctaattga aaacggtaac aaaactaatt
      241 tagagaatga aatatagagt atttctctct taaaagtttt tggtgaaacg agatgtagaa
      301 aggagattta gccaaagagt ctattagtgc tagaataata gattagaatt attttagaaa
      361 aacgaagtga gcagcttata aattcaagtc cccaaataga ttcatactag tatcttttgc
      421 aaaaaataaa gggcgacttc cttcatgaat atcaatttca tctataagga aggtagctaa
      481 ttgaactaac ttatttattc tgtttgtcgc tagaaaaatc agacctcctt gtgaagattg
      541 aggagatact gaatgaaaat caaagaagaa actagcaagc tagtagcaga ttgcccaaaa
      601 caccgctttg aggttgtaga taagactgac gtatataatc caaggtgaag cgactgtggt
      661 ttgaagagat tttcaaagag tataggctag agagtagtgt ttttatgtcc ttctagtaga
      721 aaatgctaga cagaagaatg gggaacttgg ataggagaaa tagattgaga aaggaggtta
      781 gaagagatga ttattacaaa aattagccgt ttaggaactt atgtgggagt aaatccacat
      841 tttgcaacat taatagattt tctagaaaaa acaggactag aaaatttaac agaaggttcg
      901 attgctatcg atggtaatcg attgtttggg aattgcttta cttatctagc agatggtcaa
      961 gcaggggctt tctttgaaac acaccaaaaa tatttggata ttcatttagt tttggaaaac
     1021 gaagaagcca tggctgttac atcgccggaa aatgtaagcg ttacccaaga atatgatgaa
     1081 gagaaagata ttgaattata cacagggaaa gtggaacagt tggttcattt gagagctggt
     1141 gaatgcctca tcacttttcc agaagattta catcaaccca aggttcgtat aaatgatgaa
     1201 cctgtgaaaa aagttgtctt taaagttgcg atttcttaat gtagaaagag aagaacgatg
     1261 aaaaaaatga gaaagttttt atgtctagct ggaattgcgc tagcggctgt tgccttggta
     1321 gcttgttcag gaaaaaaaga agctacaact agtactgaac caccaacaga attatctggt
     1381 gagattacaa tgtggcactc ctttactcaa ggaccccgtt tagaaagtat tcaaaaatca
     1441 gcagatgctt tcatgcaaaa gcatccaaaa acgaaaatca agattgaaac attttcttgg
     1501 aatgacttct atactaaatg gactacaggt ttagcaaatg gaaatgtgcc agatatcagt
     1561 acagctcttc ctaaccaagt aatggaaatg gtcaactcag atgctttggt tccgctaaat
     1621 gattctatca agcgtattgg acaagataaa tttaacgaaa ctgccttaaa tgaagcaaaa
     1681 atcggagatg attactactc tgttcctctt tattcacatg cacaagtcat gtgggttaga
     1741 acagatttgt taaaagaaca taatattgag gttcctaaaa cttgggatca actctatgaa
     1801 gcttctaaaa aattgaaaga agctggagtt tatggcttgt ctgttccgtt tggaacaaat
     1861 gacttaatgg caacacgttt cttgaacttc tacgtacgta gtggtggagg aagcctctta
     1921 acaaaagatc ttaaagcaga cttgacaagc caacttgctc aagatggtat taaatactgg
     1981 gttaaattgt ataaagaaat ctcacctcaa gattctttga actttaatgt ccttcaacaa
     2041 gctaccttgt tctatcaagg aaaaacagca tttgacttta actctggctt ccatatcgga
     2101 ggaattaatg ccaacagtcc tcaattgatt gattcgattg atgcttatcc tattccaaaa
     2161 atcaaagagt ctgataaaga ccaaggaatt gaaacctcaa acattccaat ggttgtttgg
     2221 aaaaattcaa aacatccaga agttgctaaa gcattcttag aagcacttta taatgaagaa
     2281 gactacgtta aattccttga ttcaactcca gtaggtatgt tgccaactat tagggggatt
     2341 agcgattctg cagcctataa agaaaatgaa actcgtaaga aatttaaaca tgctgaagaa
     2401 gtaattactg aagctgttaa aaaaggtact gctattggtt atgaaaatgg accaagtgta
     2461 caagctggta tgttgactaa ccaacacatt attgaacaaa tgttccaaga tatcattaca
     2521 aatggaacag atcctatgaa agcagcaaaa gaagcagaaa aacaattaaa tgatttattt
     2581 gaggctgttc agtagatgta aaagactaga aaataggtgg gctagtgagc tgaaaagctc
     2641 tagcccaatc ttgtaaaaga agggagaagg agaatggtta aagaacgtaa tttaactcgc
     2701 tggatatttg ttttgccagc tatgattatc gtaggattac tctttgttta tccgtttttc
     2761 tcgagtattt tttatagctt taccaataag catttgatta tgcctaatta taaatttgtt
     2821 ggtttggcta actataaagc tgtgctatca gatcccaact tctttaatgc gttctttaat
     2881 tcaattaagt ggaccgtttt ctcattagtt ggtcaagttt tagtagggtt tgtgttggct
     2941 ttagctcttc acagagtacg ccacttcaag aaattatata ggaccttatt gattgttcct
     3001 tgggcatttc ctaccatcgt tattgccttc tcttggcagt ggattctaaa cggggtttat
     3061 ggctacttac ctaatctaat cgtaaaatta ggtttaatgg aacatacacc tgcatttttg
     3121 acagatagta catgggcatt cctatgtttg gtgtttatca acatttggtt tggagcacca
     3181 atgattatgg ttaatgtgct ttcagctttg caaacagtac cagaagaaca atttgaggct
     3241 gctaagatag atggtgcttc aagttggcag gtgttcaagt ttatcgtctt tccacatatt
     3301 aaagtggttg taggacttct agttgttttg agaactgtat ggatctttaa taactttgac
     3361 attatctacc tcattactgg tggtggacca gccaatgcta caacgacgct tccaattttt
     3421 gcttacaacc tgggctgggg aactaaattg ttgggtcgtg cttcagcagt tacagtattg
     3481 ctctttatct tcttggtggc gatttgcttt atctactttg ctatcatcag taagtgggaa
     3541 aaggagggta gaaaataatg aagaagaaat ccagtattta tttagatatt ctctcacatg
     3601 tacttttagt tggtgcgacc atcgttgcag ttttcccatt ggtatggatt atcatatctt
     3661 ctgtcaaagg gaaaggggaa ttaactcagt atccaacacg attttggcct gaacagttta
     3721 cattagatta tttcactcat gttatcaacg atttgcactt cattgataac attcgaaaca
     3781 gtttaatcat tgccttggct acaaccctca ttgcgattat tatttctgct atggcagcct
     3841 atggtattgt tcgattcttt cctaaattgg gagcaatcat gtcgagacta ctcgtcatta
     3901 cctatatttt cccaccaatt ttgttagcaa ttccctattc aattgccatt gctaaagttg
     3961 ggttaacaaa tagtttattt ggcttgatga tggtttatct atcttttagt gttccatatg
     4021 cagtttggct cttagttgga tttttccaaa cagttccaat tggaattgaa gaagcggcta
     4081 gaattgatgg tgcaaataaa tttgttacgt tttataaagt tgtgctaccg attgtagcac
     4141 caggtattgt agcaacagct atttatacat ttatcaatgc ttggaatgaa ttcctgtatg
     4201 ccttgatttt gattaacaat acaggaaaga tgacagtagc agtagccctt cgttcactta
     4261 atggttcaga aatactagac tggggagata tgatggcagc gtctgttatt gtagttcttc
     4321 catcaattat tttcttctct atcatccaaa ataagattgc aagtggatta tcagaaggat
     4381 ctgtgaagta gacgaaagaa ggaaaaaaat gaataaaaga ggtctttatt caaaactagg
     4441 aatttctgtt gtaggcatta gtcttttaat gggagttccc actttgattc atgcgaatga
     4501 attaaactat ggtcaactgt ccatatctcc tatttttcaa ggaggttcat atcaactgaa
     4561 caataagagt atagatatca gctctttgtt attagataaa ttgtctggag agagtcagac
     4621 agtagtaatg aaatttaaag cagatgaacc aaactctctt caagctttgt ttggcctatc
     4681 taatagtaaa gcagacttta aaaataatta cttttcaatt ttcatgagag attctggtga
     4741 gataggtgta gaaataagag acgcccaaaa gggaataaat tatttatttt ccagaccagc
     4801 ttcattatgg ggaaaacata aaggacaggc agttgaaaat acactagtat ttgtatctga
     4861 ttctaaagat aaaacataca caatgtatgt taatggaata gaagtgttct ctgaaacagt
     4921 tgatacattt ttgccaattt caaatataaa tggtatagat aaggcaacac taggagctgt
     4981 taatcgtgaa ggtaaggaac attacctcgc aaaaggaagt attgatgaaa tcagtctatt
     5041 taacaaagca attagtgatc aggaagtttc aaatattccc ttgtcaaatc catttcagtt
     5101 aattttccaa tcaggagatt ctactcaagc taactatttt agaataccga cactatatac
     5161 attaagtagt ggaagagttc tatcaagtat tgatgcacgt tatggtggga ctcatgattc
     5221 taaaagtaag attaatattg ccacttctta tagtgatgat aatgggaaaa cgtggagtga
     5281 gccaattttt gctatgaagt ttaatgacta tgaggagcag ttagtttact ggccacgaga
     5341 taataaatta aagaatagtc aaattagtgg aagtgcttca ttcatagatt catccattgt
     5401 tgaagataaa aaatctggga aaacgatatt actagctgat gttatgcctg cgggtattgg
     5461 aaataataat gcaaataaag ccgactcagg ttttaaagaa ataaatggtc attattattt
     5521 aaaactaaag aagaatggag ataacgattt ccgttataca gttagagaaa atggtgtcgt
     5581 ttatgatgaa acaactaata aacctacaaa ttatactata aatgataagt atgaagtttt
     5641 ggagggagga aagtctttaa cagtcgaaca atattcggtt gattttgata gtggctcttt
     5701 aagagaaaaa cataatggaa aacaggttcc tatgaatgtt ttctacaaag attcgttatt
     5761 taaagtgact cctactaatt atatagcaat gacaactagt cagaatagag gagagagttg
     5821 ggaacaattt aagttgttgc ctccgttctt aggagaaaaa cataatggaa cttacttgtg
     5881 tcctggacaa ggtttagcat taaaatcaag taacagattg atttttgcaa catatactag
     5941 tggagaacta acctatctca tttctgatga tagtggtcaa acatggaaga aatcctcagc
     6001 ttcaattccg tttaaaaatg caacagcaga agcacaaatg gttgaactga gagatggtgt
     6061 gattagaaca ttctttagaa ccactacagg taagatagct tatatgacta gtagagattc
     6121 tggagaaaca tggtcggaag tttcgtatat tgatggaatc caacaaactt catatggcac
     6181 acaagtatct gcaattaaat actctcaatt aattgatgga aaagaagcag tcattttgag
     6241 tacaccaaat tctagaagtg gccgtaaggg aggtcaatta gttgtcggtt tagtcaataa
     6301 agaagatgat agtattgatt ggaaatacca ctatgatatt gatttgcctt cgtatggtta
     6361 tgcctattct gcgattacag aattgccaaa tcatcacata ggtgtactgt ttgaaaaata
     6421 tgattcgtgg tcgagaaatg aattgcattt aagcaatgta gttcagtata tagatttgga
     6481 aattaatgat ttaacaaaat aaaggagaaa aacatggtta aatacggtgt tgttggagca
     6541 gggtattttg gagctgaatt ggctcgctac atgcaaaaaa atgatggagc agagattact
     6601 cttctctatg atccagataa tgcagaggcg attgcagaag aattgggagc aaaagtagca
     6661 agttccttag atgagttggt ttctagcgat gaagtagact gtgttatcgt cgcaactcca
     6721 aataatcttc ataaggaacc ggttattaag gctgcacagc atggtaaaaa tgttttctgt
     6781 gaaaaaccaa ttgcgctttc ttatcaagat tgtcgcgaga tggtagatgt gtgtaaagaa
     6841 aacaatgtaa cctttatggc aggacatatt atgaatttct ttaatggtgt tcatcatgca
     6901 aaagaactca ttaatcaagg agttatcgga gacgttctat attgtcatac agctcgtaat
     6961 ggttgggaag aacaacaacc gtcagtatca tggaaaaaaa ttcgtgaaaa atcaggtggt
     7021 cacttgtatc accacatcca tgaattggat tgcgttcaat tccttatggg gggcatgcct
     7081 gaaactgtaa ccatgacagg tggaaatgtg gcccatgaag gtgaacattt cggtgatgaa
     7141 gatgatatga tttttgtcaa tatggaattt tctaataagc gttttgcttt gttagaatgg
     7201 ggttcagctt atcgttgggg tgaacattat gtcttaatcc aaggaagcaa aggtgccatc
     7261 cgcttagact tattcaactg taaaggaact cttaagctag atgggcaaga aagctatttc
     7321 ttgattcacg aatcgcaaga agaagatgat gatcggactc gtatctatca tagtacagag
     7381 atggatggag caattgctta tggtaaacca ggtaaacgta ctccattatg gctatcatct
     7441 gtcattgata aagaaatgcg ctatctgcat gagattatgg aaggagctcc agtatcagaa
     7501 gaatttgcaa aacttttgac aggtgaagct gccctagaag caattgctac tgcagatgct
     7561 tgtacccagt ctatgtttga agatcgcaaa gtaagattgt cagaaattgt aaaataaatt
     7621 ttggtattct cctatctata ggtcgacttg ctcctctgaa agtactttta gaggagctgt
     7681 ttgactttgc tagtttttga aactgaaatc tattatacta caaactattg aaagcgtttt
     7741 aattttaagg tataataatc tcatagaaat aaagaaaagg aggaaagagg atgccacaga
     7801 ttagcaaaga agccttgatt gagcaaatca aagatggaat catcgtttct tgtcaggctc
     7861 ttcctcatga accgctttat acagaagcgg gaggggtgat tcccttgctg gtcaaagcgg
     7921 ctgagcaagg tggagcagtc ggtatccgag caaacagtgt tcgcgatatc aaggaaatta
     7981 aggaagtcac taaacttcca atcattggga ttatcaaacg tgattatcca cctcaggaac
     8041 ccttcatcac ggctactatg aaagaagttg atgaattggc agaactggac atcgaggtga
     8101 ttgctctgga ttgtaccaag cgtgaacgct acgatggttt ggaaattcaa gagttcattc
     8161 gtcaggttaa ggagaaatat cctaatcagc ttttgatggc tgatactagt atcttcgaag
     8221 aagggctagc agctgtagaa gcaggaattg actttgtcgg aacaacctta tcaggctaca
     8281 catcctacag tccaaaagta gacggtccag attttgaatt gattaagaaa ctctgtgatg
     8341 ccggtgtaga tgtcattgca gaaggaaaaa ttcatacacc agaacaagcc aaacaaatcc
     8401 ttgaatatgg agtgcgaggc atcgttgttg gtggcgccat tactagacca aaagagatta
     8461 cagaacgctt cgttgctagt cttaaataag atgtgagggg gagttttatg tttaaagttt
     8521 tacaaaaagt tggaaaagct tttatgttac ctatagctat acttcctgca gcaggtctac
     8581 ttttggggat tggtggtgca ctttcaaacc caaccacgat agcaacttat ccaatactag
     8641 acaatagtat ttttcaatca atattccaag taatgagctc tgcaggagag gttgtattca
     8701 gtaatttgtc actacttctc tgtgtgggat tatgtattgg cttagcgaaa cgagataaag
     8761 gaaccgctgc gttagcagga gtaactggtt acttagttat gactgcaacg atcaaagctt
     8821 tagtaaaact ttttatggca gaaggatctg caattgatac tggagttatt ggagcattag
     8881 ttgtcggaat agttgccgta tatttgcaca accgatataa caatattcaa ttaccttccg
     8941 ctttaggatt ctttggaggt tcacgcttcg ttcctattgt tacatcgttc tcttctatct
     9001 tgattggctt tgtcttcttt gttatttggc cacctttcca acaacttctt gtttctacag
     9061 gtggatatat ttctcaggcg ggtccaattg gaacttttct atatggattt ttaatgagac
     9121 tttctggagc agtaggctta catcatataa tttaccctat gttttggtat actgaacttg
     9181 gtggtgttga aactgttgca ggacaaacag tggttggagc tcaaaaaata ttttttgctc
     9241 aattagccga tccggcccat tctggattat ttacagaagg aacaaggttt tttgcaggtc
     9301 gtttctcaac aatgatgttc ggtttaccgg ctgcctgttt agcgatgtac catagtgttc
     9361 ctaaaaatcg tcgtaaaaaa tacgcgggtt tgttttttgg agttgcttta acatctttta
     9421 ttaccggtat tacagaacca attgaattta tgtttctatt cgtcagtccg gttctatatg
     9481 ttgttcacgc attccttgat ggtgttagct tctttattgc agacgtctta aatatttcaa
     9541 taggaaacac attttcagga ggtgtaatcg atttcacatt atttggaatt ttgcagggga
     9601 acgctaagac gaattgggtt cttcagattc catttggact tatttggagt gttttgtatt
     9661 atattatttt tagatggttc attactcaat tcaacgttct aacgccaggg cgaggagaag
     9721 aagtagattc taaagaaatt tctgaatccg cagattcaac ttcaaatact gcagattatt
     9781 taaaacagga tagcctacaa attatcagag ccttgggtgg atcaaataat atagaagatg
     9841 tagatgcttg tgtgacacgt ttacgtgtag ctgtaaaaga agttaatcaa gttgataaag
     9901 cacttttaaa acaaattggt gcagttgatg tcttagaagt gaagggtggc attcaagcaa
     9961 tctatggagc aaaagcaatc ttatataaaa atagtattaa tgaaatttta ggtgtagatg
    10021 attaagtact tactgactta ataaaaaaca gaggagagtg atggatgagt aggatgaaat
    10081 gaaatcgcat acaagaaata aagaactcat tatccaagtt ggatacgctt attacatagg
    10141 agaatacaaa tgaaatttag aaaattagct tgtacagtac ttgcgggtgc tgcggttctt
    10201 ggtcttgctg cttgtggcaa ttctggcgga agtaaagatg ctgccaaatc aggtggtgac
    10261 ggtgccaaaa cagaaatcac ttggtgggca ttcccagtat ttacccaaga aaaaactggt
    10321 gacggtgttg gaacttatga aaaatcaatc atcgaagcgt ttgaaaaagc aaacccagat
    10381 ataaaagtga aattggaaac catcgacttc aagtcaggtc ctgaaaaaat cacaacagcc
    10441 atcgaagcag gaacagctcc agacgtactc tttgatgcac caggacgtat catccaatac
    10501 ggtaaaaacg gtaaattggc tgagttgaat gacctcttca cagatgaatt tgttaaagat
    10561 gtcaacaatg aaaacatcgt acaagcaagt aaagctggag acaaggctta tatgtatccg
    10621 attagttctg ccccattcta catggcaatg aacaagaaaa tgttagaaga tgctggagta
    10681 gcaaaccttg taaaagaagg ttggacaact gatgattttg aaaaagtatt gaaagcactt
    10741 aaagacaagg gttacacacc aggttcattg ttcagttctg gtcaaggggg agaccaagga
    10801 acacgtgcct ttatctctaa cctttatagc ggttctgtaa cagatgaaaa agttagcaaa
    10861 tatacaactg atgatcctaa attcgtcaaa ggtcttgaaa aagcaactag ctggattaaa
    10921 gacaatttga tcaataatgg ttcacaattt gacggtgggg cagatatcca aaactttgcc
    10981 aacggtcaaa catcttacac aatcctttgg gcaccagctc aaaatggtat ccaagctaaa
    11041 cttttagaag caagtaaggt agaagtggta gaagtaccat tcccatcaga cgaaggtaag
    11101 ccagctcttg agtaccttgt aaacgggttt gcagtattca acaataaaga cgacaagaaa
    11161 gtcgctgcat ctaagaaatt catccagttt atcgcagatg acaaagagtg gggacctaaa
    11221 gacgtagttc gtacaggtgc tttcccagtc cgtacttcat ttggaaaact ttatgaagac
    11281 aaacgcatgg aaacaatcag cggctggact caatactact caccatacta caacactatt
    11341 gatggatttg ctgaaatgag aacactttgg ttcccaatgt tgcaatctgt atcaaatggt
    11401 gacgaaaaac cagcagatgc tttgaaagcc ttcactgaaa aagcaaacga aacaatcaaa
    11461 aaagctatga aacaatagtc cttagttatt ctataaaaag tagtttttta aagaacctaa
    11521 gagtgtatac ccccttttcc ctctacacag atagtgtaag aaaagggggc ttttgtttaa
    11581 aatgtaagaa actgtcacga aattaaaatg aagttcttac ataagcgaat cataaaaaat
    11641 ttcattttga ttttaaaaca gttcaagaaa gtcaaaaaat tattctattt gaaagagagg
    11701 tgccgactgt gaaagtcaat aaaatccgta tgcgggaaac agtgatttcc tacgctttcc
    11761 tagcaccagt attattcttc tttgtcatct ttgtgttggc tccgatggtg atgggcttca
    11821 ttacaagttt ctttaactac tcaatgacta aatttgagtt tgtaggcttg gataactata
    11881 tccgtatgtt taaagatcct gtctttacaa aatctctgat taacacagtt attttggtta
    11941 ttggatctgt accagttgtt gttctattct cactctttgt agcatctcag acctatcatc
    12001 aaaatgtcat tgccagatcc ttctaccgtt tcgtcttctt ccttcctgtt gtaacgggta
    12061 gtgttgccgt gacagttgtt tggaaatgga tttatgaccc gctatcaggg attctaaact
    12121 ttgtccttaa gtcaagccac atcatcagcc aaaacatttc ttggttggga gataaaaact
    12181 gggcattgat ggcgattatg attattctct tgaccacttc agttggtcag cccatcatcc
    12241 tttatatcgc tgccatgggg aatattgaca attcactggt tgaagcggcg cgtgttgatg
    12301 gtgcaactga gtttcaagtt ttttggaaga ttaaatggcc aagccttctt ccaacaactc
    12361 tttatattgc aatcatcaca acaattaact cattccagtg tttcgccttg attcagcttt
    12421 tgacatctgg tggtccaaac tactcaacaa gtaccttgat gtactacctt tacgaaaaag
    12481 ccttccaatt gacagaatac ggctatgcca acacaattgg tgtcttcttg gcagtcatga
    12541 ttgctatcgt aagctttgtt caatttaaag tacttggaaa cgacgtagaa tactaaagaa
    12601 aggagacagc tatgcaatct acagaaaaaa aaccattaac agcctttact gttatttcaa
    12661 caatcatttt gctcttgttg actgtgctgt tcatctttcc attctactgg attttgacag
    12721 gggcattcaa atcacaacct gatacaattg ttattcctcc tcagtggttc cctaaaatgc
    12781 caaccatgga aaacttccaa caactcatgg tgcagaaccc tgccttgcaa tggatgtgga
    12841 actcagtatt tatctcattg gtaaccatgt tcttagtttg tgcaacctca tctctagcag
    12901 gttatgtatt ggctaaaaaa cgtttctatg gtcaacgcat tctatttgct atctttatcg
    12961 ctgctatggc gcttccaaaa caagttgtcc ttgtaccatt ggtacgtatc gtcaacttca
    13021 tgggaatcca cgatactctc tgggcagtta tcttgccttt gattggatgg ccattcggtg
    13081 tcttcctcat gaaacaattt agtgaaaata ttcctacaga gttgcttgaa tcagctaaaa
    13141 tcgacggttg tggtgagatt cgtaccttct ggagtgtagc cttcccgatt gtgaaaccag
    13201 ggtttgcagc ccttgcaatc tttaccttca tcaatacttg gaatgactac ttcatgcaat
    13261 tggtaatgtt gacttcacgt aacaatttga ccatctcact tggggttgcg accatgcagg
    13321 ctgaaatggc aaccaactat ggtttgatta tggcaggagc tgcccttgct gctgttccaa
    13381 tcgtcacagt cttcctagtc ttccaaaaat ccttcacaca gggtattact atgggagcgg
    13441 tcaaaggata atactctgcg aaaatcgaat gcaaactacg tcagcttcac cttgccatac
    13501 ttaagtattg cctgcggtta gcttcctagt ttgttcttca attttcattg agtataggaa
    13561 aatcaatcta tcaagataca gaagtatatt ttatagattt agagaatata gaggttataa
    13621 gtgtctacaa aatggagggt atgcagttac tttatgaagt tttgtcagac acttatcaac
    13681 ttaagaatgg ttttagttaa ctatcagaaa cgaaggaaag agtatgattt ttgacgattt
    13741 gaaaaacatc accttttaca aagggattca tcccaattta gacaaggcta tcgactacct
    13801 ctaccaacat cgtaaagatt cattcgaatt aggaaagtat gagattgatg gggataaagt
    13861 ctttctagtt gttcaggaaa atgtcctcaa tcaagttgag aataatcaat ttgaacacca
    13921 taagaactat gcagatttgc atttgctgat agaagggcat gaatattcga gctacggttc
    13981 acgtatcaaa gacgaggcag tagcattcga cgaagcgagt gacattggct ttgttcattg
    14041 tcatgaacac tacccactct tattgggtta tcacaatttt gcgattttct tcccaggtga
    14101 gccacatcag ccaaatggtt atgcaggcat ggaagaaaag gttcgaaaat atctctttaa
    14161 aattttgatt gattaaaaat aggatgaatt gtttttttgt aaagctttga caatactcta
    14221 ccatgaaatt gatctttgtg aggtagagaa atgagaataa aatatttaaa aattggtatc
    14281 ttctaagtat gctgcaagag ccagtttctt agatggacag gggattacag ttgatgagat
    14341 gtcctggata attaatggca ttgtgaatgg attgattggt agatacataa aattaggtat
    14401 ttatgcagcc aagtatggta ttagtatggt gcgatcgatc ttaagtaggg cagctgcaac
    14461 tgcggcagca agagtaggat tattgaccaa gatttttgga tggattttac gaatagctgt
    14521 gctcgcagct gatgtatatg gtaattttgc caacaatatt gctgtagctt gggatgcaca
    14581 tgataaaatc cctaacaatg gtcgtataaa cttttaaaat gcgagaatga aagcactttg
    14641 tattttttta ttgaatatgt tagcttggac agtgcttgca atgacagtcc gtggagggct
    14701 agatggattt aatgagcata cttggagtac tattttaatt gcttcgctgt ccgggatata
    14761 tatagattat atgtccatag ataaaaatag aaaaaagtcc aaaagaaaaa atagatttgt
    14821 tcatggtagg gacttatgaa agctttactg acaaaaaaga aaacagttta caaagaaaaa
    14881 tgatggagga gcaaacatgg cacaaaaagg agtaagcctt atcaaggcag catttgatac
    14941 agataacttt ctcatgcgtt ttagtgagaa agtcttggac atcgtgacag ccaatcttct
    15001 ttttgtcgtc tcttgtttgc ccatcgtgac gattggagtg gctaaaatca gcctctacga
    15061 gaccatgttc gaagttaaga agagcagacg ggtgcctgtt tttaaaatct atctaagatc
    15121 tttcaagcaa aatctgaaac taggtcttca gctgggttta atggagttag gaattgtgtt
    15181 tcttaccctt tcagatctct atcttttctg gggtcaaaca gctctgccct tccaattgct
    15241 gaaagccatt tgtttaggta ttctgatttt tcttactatc gtgatgctgg ctagttaccc
    15301 tatcgcggca cgttatgacc tatcttggaa agaaattctt caaaaaggat tgatgttggc
    15361 tagttttaac tttccttggt tcttcctcat gttagccatt cttgtcctca ttgtgatggt
    15421 tctttatctg tccgccttca gtctactctt aggtggctca gtcttcttac tttttgggtt
    15481 tggactattg gtctttatcc agactggatt gatggagaaa attttcgcaa aataccaata
    15541 ggagccttat ttctgaaact actttcaaac gctccaaacg ctattctata agcgagaaac
    15601 taaaatctaa gtgtctataa gatattgaaa gccttttcgg taaagtgtat actaaagaag
    15661 taaagaaaaa tctgctttag cagaaaataa aaataatagg agaaaatcta tgtcagattt
    15721 gaaaaaatac gaaggtgtca ttccagcctt ctacgcatgt tatgatgatc aaggagaagt
    15781 aagcccagaa cgtacgcgtg ccttggttca atacttcatt gataaaggtg ttcaaggtct
    15841 ttatgtcaat ggttcttctg gtgaatgtat ctaccaaagc gttgaagatc gcaagttgat
    15901 tttggaagaa gtcatggcgg tagccaaagg taaattgacc attattgctc atgttgcctg
    15961 caacaatact aaagacagta tggaacttgc tcgccatgct gaaagcttgg gagtagatgc
    16021 tattgcaacg attccaccaa tttatttccg cttgccagaa tactcagttg ccaaatactg
    16081 gaacgatatc agttctgcag ctccaaacac agactacgtg atttacaaca ttcctcaatt
    16141 ggcaggggtt gctttgactc caagccttta cacagaaatg ttgaaaaatc ctcgtgttat
    16201 cggtgttaag aactcttcta tgccagttca agatatccaa acctttgtca gccttggtgg
    16261 ggaagaccat atcgtcttta atggtcctga tgagcagttc ctaggaggac gcctcatggg
    16321 tgctagggct ggtatcggtg gtacttatgg tgctatgcca gaactcttct tgaaactcaa
    16381 tcagttgatt gcggataagg acctagaaac agcgcgtgaa ttgcagtatg ctatcaacgc
    16441 aatcattggt aaactcactt ctgctcatgg aaatatgtac ggtgtcatta aagaagtctt
    16501 gaaaatcaat gaagtcttga atattggatc tgttcgttca ccattgacac cagtgactga
    16561 agaagatcgt ccagttgtag aagcggctgc tgccttgatt cgtgaaacca aggagcgctt
    16621 cctctaatct aaaaggaggt atttatgaca tactacgttg caattgatat cggtggaacc
    16681 aacatcaagt atggtttggt tgatcaagag gggcaacttc ttgaatcgca tgaaatgcca
    16741 actgaggcgc ataagggtgg acctcatatc ttacaaaaga ccaaagatat cgtagctagt
    16801 tatttagaaa aaggcccagt agcaggtgtt gccatatctt ctgctgggat ggtggatccg
    16861 gataagggtg agattttcta tgctgggcct caaatcccta actacgcagg cacccagttc
    16921 aaaaaggaaa tcgaagaaag ctttactatt ccttgtgaga ttgaaaatga tgtcaactgt
    16981 gcaggtcttg ctgaggcagt atctggttca ggcaagggag caagtgtgac actttgcttg
    17041 accattggaa ccggtatcgg tggttgcttg attatggata ggaaagtctt ccatggtttt
    17101 agtaattcag cctgtgaagt cgggtatatg catatgcagg atggagcttt tcaggatctc
    17161 gcttctacaa cagctttagt gaaatatgta gctgaagccc atggagaaga tgttgatcag
    17221 tggaatggcc gacgtatctt taaggaagct actgaaggaa acaaaatctg catggaaggt
    17281 attgaccgta tggttgacta tctaggaaaa ggtctggcaa atatttgtta tgtggccaat
    17341 ccagaagtgg ttattcttgg tggtggtatc atggggcaag aggctatcct caaacctaag
    17401 atccgcacag ccttgaaaga ggcattggta ccaagtttag cagaaaaaac acgattagaa
    17461 tttgcccatc accaaaatac agcagggatg ttgggtgcat attatcattt taagacaaaa
    17521 caatcctagt ttggctcagc caaactagga ttttcttaca cgtttttgtc tacgatagcc
    17581 gttgagtttt ttattttccc agtagctatt aaagattttt tccttgcttt cgcgattgat
    17641 ttccaaaaag taggcataaa tcaaatcgat aaagaagagc ataggaagtt gagcggatat
    17701 tcgttggatg taggagggtt ggctgtgggt ggctacaaga acagtctctg tataggtctg
    17761 gctatcttta ttgggaacac ttgtaaagag tacagtcttt gcccccatct ccttggcatc
    17821 taatagacta tctaaaatag aaggagttga gcctgaaagt gagaaaccca gtactagaca
    17881 attttcatcc atgatgctgg ttgtccaggc aaagccgtct tggtctgtca aagcttcgca
    17941 gaccacacct agtcgcataa aacgtaattt catttcacgg gcgacgaggc cagaactccc
    18001 tgttccaaag aagtagatac gctcagcatc ttcgattagc tgggcaattc gctctagttg
    18061 gatttcgtca atcaagtctt gtgtttgttc cctcatattg ctataacttc tgaggactcg
    18121 tttggtcagt ggactatgct tggagacttg gttggcttga ttttctgcct gatgttggta
    18181 ttggaaaata aattctcggt agccagtaaa gccacacttt ttagcaaagc gggtcaaagc
    18241 agcttgagaa atatgtaatt tttgggtgac ttgttgagaa gataaatcat ctgtaatcgt
    18301 ttcagcttgc aaaaaatagc gagcgatttc ttgctctagg tctgtcattt cttcaaaatg
    18361 tgaatcaatg atagttgcga tatctggttt gtccataggg aaagctcctt tacatgggtc
    18421 atactggaag actagatcag ggaatagtca cacttcatta taacacataa tataaggata
    18481 gataaataaa aacgcatctc tgttttaaaa acgaaaaaat cgaaaaagct tctctctttt
    18541 ccataatttt ctactcaaat tgtggtacaa ttaagagtaa gattttaagt tagaaatgag
    18601 actgatttgt atgagaaaat ttaacagcca ttcgattccg attcggctta atttattgtt
    18661 ttcaatcgtc attttactct ttatgaccat tattggtcgt ttgttgtata tgcaggtttt
    18721 gaacaaggat ttttacgaaa aaaagctagc ctcagctagt cagaccaaga ttacaagcag
    18781 ttcagcccgt ggggaaattt atgatgctag tggaaaacct ttggtagaaa atacgttaaa
    18841 gcaggttgtt tcctttacgc gtagcaataa aatgacggct acagacttaa aagaaacagc
    18901 taaaaagtta ctgacttatg tgagcatcag ttctccaaat ttgacagaac gccagctggc
    18961 ggattactat ttggctgatc ctgaaatcta taaaaaaaca gtggaagctc tcccaagtga
    19021 gaaacgcttg gattcagatg gcaatcgtct atccgaatca gaactgtata acaatgcggt
    19081 cgatagtgtc caaacaagtc aactaaacta tacagaggat gaaaagaaag aaatctatct
    19141 ttttagtcag ttaaatgctg ttggaaactt tgcgacagga accattgcta cagatcctct
    19201 aaatgattct caggtggctg ttattgcctc tatttcaaag gagatgcctg gcattagtat
    19261 ttctacttct tgggatagaa aggttttgga aacttccctt tcttctatag ttgggagtgt
    19321 atccagtgaa aaagctggtc tcccagcgga agaagcagaa gcctatctta aaaaaggcta
    19381 ttctctaaat gatcgtgtag gaacctccta tttggaaaag caatatgaag agaccttaca
    19441 aggaaaacgc tcggtaaaag aaatccatct ggataaatat ggcaatatgg aaagcgtgga
    19501 tacaattgag gaaggtagta agggaaacaa tatcaaactg accattgatt tggctttcca
    19561 agatagcgtg gatgctttac tgaaaagtta tttcaattcc gagctagaaa atggtggagc
    19621 caagtattct gaaggtgtct atgcagtcgc ccttaaccca aaaacaggtg cggttttgtc
    19681 tatgtcaggg attaaacatg acttgaaaac gggagagttg acgcctgatt ccttgggaac
    19741 ggtaaccaat gtctttgttc caggttcggt tgtcaaggcg gcgaccatca gctcaggttg
    19801 ggaaaatgga gtcttgtcag gaaaccagac cttgacagac cagtccattg tcttccaagg
    19861 ttcagctccc atcaattctt ggtatactca ggcttacggt tcattcccta tcacagcggt
    19921 ccaagctctg gagtattcat caaataccta tatggtccaa acagccttag gtcttatggg
    19981 gcaaacctat caacccaata tgtttgtcgg caccagcaat ctagagtctg ctatggagaa
    20041 actgcgttca acctttggcg aatatggctt gggtactgcg acaggaattg acctaccaga
    20101 tgaatctact ggatttgttc ccaaagagta tagctttgct aattacatta ctaatgcctt
    20161 tgggcagttt gataactata cgccgatgca gttggctcag tatgtagcaa ctattgcaaa
    20221 taatggtgtt cgtgtggctc ctcgtattgt tgaaggcatt tatggtaata atgataaggg
    20281 aggactgggt gacttgattc agcaactgca accgacagag atgaataagg tcaatatatc
    20341 cgactccgat atgagcatct tgcaccaagg tttttatcag gttgcccatg gtactagtgg
    20401 attgacaact ggacgtgcct tttcaaatgg cgccttggta tccattagcg gaaaaacagg
    20461 tacagccgaa agctatgtgg cagatggtca gcaagcaacc aataccaatg cggtggccta
    20521 tgccccatct gataatcccc aaatcgctgt tgcagtggtc tttcctcata ataccaatct
    20581 aacaaatggt gtaggacctt ccattgcgcg tgacattatc aatctgtatc aaaaatacca
    20641 tccaatgaac tagaaaggaa attatgcttt atccaacacc tattgccaag ttgattgaca
    20701 gttattctaa gttaccaggt atcgggatta agacggctac gcgtctggcc ttttatacga
    20761 ttgggatgtc tgctgatgat gtcaatgaat ttgcaaaaaa tctcctttct gctaagagag
    20821 aattgacata ttgttctatt tgtggacgtt tgacagacga cgatccttgt tctatctgta
    20881 ctgatccgac tcgtgaccag acaacaattt tagttcttga ggatagtaga gatgtggcag
    20941 ccatggaaaa tatccaagaa taccatggac tctatcatgt ccttcatggc ctcatttctc
    21001 ctatgaatgg tatcagtccg gacgatatca atctcaagag ccttatgact cgtcttatgg
    21061 atagtgaagt ttcagaagtg attgtggcga ctaatgctac agcggatggt gaagcgactt
    21121 ccatgtatct ttcacgtttg ctcaagccgg ctggtatcaa ggttacgcgt ctagcacgag
    21181 gtctcgctgt gggagcggac attgagtatg cggacgaagt gacactctta cgagccattg
    21241 aaaatcggac agagttgtaa gtgtaggcaa atttacgaac ttcattcatt tataaaaaat
    21301 caaagaggct gaaaatcgtt cctatcggcc tctttttgta tagtgtgatg agtaggatca
    21361 ggttcaagtt ttaaaaaacc aagcaaatat gatatactaa agagcgagta ttctagtaga
    21421 attaggacaa ataatatgaa acaaacgatt attcttttat atggtggacg gagtgcggaa
    21481 cgcgaagtct ctgtcctttc agctgagagt gtcatgcgtg cggtcaatta cgaccgtttc
    21541 acagtcaaga ctttctttat cagtcagtca ggtgacttta tcaaaacaca ggaatttagt
    21601 catgctccgg ggcaagaaga ccgtctcatg accaatgaaa ccattgattg ggataagaaa
    21661 gttgcaccaa gtgctatcta cgaagaaggt gcagtggtct ttccagtcct tcacgggcca
    21721 atgggagaag atggctctgt tcaaggattc ttggaagttt tgaaaatgcc ttacgttggt
    21781 tgcaacattt tgtcatcaag tcttgccatg gataaaatca cgactaagcg tgttctggaa
    21841 tctgctggta ttgcccaagt tccttatgtg gctatcgttg aaggcgatga tgtgactgct
    21901 aaaatcgctg aagtggaaga aaaattggct tatccagtct tcactaagcc gtcaaacatg
    21961 gggtctagtg tcggtatttc taagtctgaa aaccaagaag aactccgtca agccttaaaa
    22021 cttgccttcc gatatgacag ccgtgtcttg gttgagcaag gagtgaatgc ccgtgaaatt
    22081 gaggttggcc tcttgggtaa ctacgatgtc aagagcacgc tacctggaga agttgtcaag
    22141 gacgttgcct tttatgacta cgatgccaag tatattgata acaagattac tatggatatt
    22201 cctgccaaaa tcagtgatga tgtggtggct gtcatgcgtc aaaatgcaga aacagccttc
    22261 cgtgccattg gtggccttgg tctatctcgt tgcgatttct tctatacaga taagggagag
    22321 atttttctca acgagctcaa taccatgcca ggttttaccc agtggtctat gtacccacta
    22381 ctttgggaca atatggggat cagctaccca gaactaatcg agcgtttggt tgaccttgcc
    22441 aaggaaagtt ttgacaagcg cgaagcgcat ttgatataaa atgaaagaga gggtagaagc
    22501 cagaaccatc actgcacggt gactagagtt ctcggacttc agcccttttt aaaggagtag
    22561 aaatgaaatt aacaatccat gaaattgccc aagttgttgg agccaaaaat gatatcagta
    22621 tctttgagga cacccagtta gaaaaagctg agtttgatag tcgtttgatt ggaactggag
    22681 atttatttgt gccacttaaa ggtgcgcgtg atggccatga ctttattaaa acagcctttg
    22741 aaaatggtgc agcagtaacc ttgtctgaga aagaggtctc aaatcatcct tacattctag
    22801 tagatgatgt tttgacagcc tttcaatccc tagcatccta ctatcttgaa aaaacgactg
    22861 ttgatgtctt tgctgttaca ggttcaaatg gcaagacaac gactaaggat atgttggcgc
    22921 atttactgtc aacaagatac aagacctaca aaacacaagg caattacaat aatgagattg
    22981 gccttcctta cacagttctc cacatgcctg aaggaacaga aaagttggtc ttggagatgg
    23041 ggcaggacca cttgggagat attcatctct tgtctgaatt ggctcgtcca aaaacagcca
    23101 tcgtgacctt ggttggagaa gctcatttgg cctttttcaa agatcgttct gagattgcta
    23161 aaggaaaaat gcaaattgca gacggaatgg cttcaggttc cttgctttta gcgccggctg
    23221 accctatcgt agaggactat ttgccaactg ataaaaaggt ggtccgtttt gggcaagggg
    23281 cagagttgga aatcacagac ttggttgagc gcaaagatag tctaaccttc aaggccaatt
    23341 tcttagagca agcccttgat ttgccagtaa ctggtaagta caatgctacc aatgctatga
    23401 ttgcatccta tgttgcccta caagaaggag tttcagagga gcaaattcat caggccttcc
    23461 aaggtcttga attgacgcgt aatcgtactg agtggaagaa agcagccaat ggagcagata
    23521 tcctgtcaga cgtatataat gccaatccaa ctgctatgaa gctgatttta gagactttct
    23581 ctgccattcc agccaatgaa ggtggcaaga aaattgcagt gttggcggat atgaaggagc
    23641 ttggtgacca gtctattcaa ctccataacc agatgatttt gagcctttct ccagatgtgc
    23701 ttgataccgt tattttctat ggagaagaca ttgctgaatt agcccaattg gccagtcaaa
    23761 tgttcccaat aggccacgtt tactacttca agaaaacaga agaccaagac caatttgaag
    23821 acctagccaa gcaggttaag gaaagccttg gagcctatga ccaaatcttg ctaaaaggtt
    23881 ctaactctat gaacctagct atgttggtag aaagtttaga aaatgaaacc aagtgatttt
    23941 gttaagtatc tgcaaagaat gattgccctt acagatactg gcttaacctt tacaaaagat
    24001 ccttttgacc gtgagcgcta cgaggacttg cgaagtctgt tatctgaaat gttgaatcag
    24061 ggatcagacc tggatgcaga agaagtagca gaagtcttga aaccaacttc agcttatgcg
    24121 actcccttaa tggacgtccg tgcttggatt gttgaggatg agaagatttg tctggttagg
    24181 ggacaaggag aggatagttg ggctttgcca ggtggctttg gtgaagtcgg ctattctcca
    24241 actgaaaata ttctcaagga aattgaagaa gaaaccggtt ttaaagccaa agttgaaaga
    24301 ctgctagctg tttttgatac caatcgtttc caactacaga gcaaacaata tgcaaagttt
    24361 gtttttgaat gtaagcttct cgatggacaa ttccaagaaa atcaagaaat cgctgacctt
    24421 caattttttg caattgacca actgccgaat ttatctgaaa aacgcattac caaggagcaa
    24481 atcgagattc tttggcaggt ttatcaaggt cagagggagc aatatcttga ctaagaagat
    24541 gattatcgta tttctaaatc catttttgac aactagcatg gtataataat atgcaggaaa
    24601 attttgaatt atgaggaaga ctagatgaat ttatgggata ttttctttac gactcaggca
    24661 accgagccgc ccaaatttga ccttttttgg tatgttagcc tatttacgct cttagcctta
    24721 accttttata cagcccatcg ctatcgtgaa aagaaggttt accaacgatt tttccaaatc
    24781 ttgcagactg ttcagttaat ccttctttat ggttggtact gggtcaatca tatgccactg
    24841 tcagaaagcc taccctttta ccattgccgt atggctatgt ttgtggtact cttgcttcct
    24901 ggtcagtcca aatataaaca atactttgca ttattgggaa catttgggac attagcagcc
    24961 tttgtttatc cagtgccaga tgcttaccct tttccacata tcaccattct atcctttatc
    25021 tttggtcatt tagcactctt ggggaactct ctagtttatc tattgagaca gtataatgcg
    25081 cgattgctgg atgtgaaggg aatttttctc atgacctttg ccctaaatgc cttgattttt
    25141 gtggtcaatt tggtgacagg tggcgattac ggatttttga caaaaccgcc attggttggg
    25201 gatcacggtc tagtagctaa ttatttactt gtttcaattg tgctggtagc tactatcagt
    25261 ttgactaaga aaatcttaga attcttttta gctcaagaag cagaaaaaat gattgcaaag
    25321 gaagcttaac acagagcttt cttttttgct cttagagagt ttttacaagc agcttataaa
    25381 ataagaattt ttgaatagac aaactcaaaa aatggctggg aaatttagga aaaaagcaag
    25441 cacgattaaa ttttttgtgt tataatattt tgtgaatagc tatgcctatg tttagctatg
    25501 gaataatacg aagtgcgaaa cttggaagat agagaggaag cgatgtaatg gctagagaag
    25561 gcttttttac aggtctagat attggaacaa gctctgtcaa ggtgcttgtg gccgagcaga
    25621 gaaatggtga attaaatgta attggcgtga gtaatgccaa aagtaaaggt gtaaaggatg
    25681 gaattattgt tgatattgat acagcagcaa ctgctatcaa gtcagccatt tcccaagcgg
    25741 aagaaaaggc aggcatttcg attaaatcag tgaatgtcgg cttgcctggt aatcttttgc
    25801 aggtagaacc aactcagggg atgattccag taacatctga tactaaggaa attacggatc
    25861 aagatgttga aaatgttgtc aaatcagctt tgacaaagag tatgacacct gaccgtgaag
    25921 tcattacctt tattcctgaa gaatttattg tggatggttt ccaagggatt cgtgacccac
    25981 gtggtatgat gggggttcgc cttgaaatgc gtggtttgct ttatacagga cctcgtacta
    26041 tcttgcacaa tttgcgtaag acggttgagc gtgcaggtgt tcaggttgaa aatgttatca
    26101 tttcaccact agcaatggtt cagtctgttt tgaatgaagg ggaacgtgaa tttggtgcta
    26161 cagtgattga tatgggggca ggtcaaacga ctgtcgctac aatccgtaat caagaactcc
    26221 agttcacaca tattctccaa gaaggtggag attatgtaac taaagatatc tccaaggttt
    26281 tgaaaacctc tcgcaaatta gcggaaggct tgaaactgaa ttacggggaa gcctatccgc
    26341 ctcttgcaag caaagaaacc ttccaagtag aggttattgg agaagtggaa gcagtcgaag
    26401 tgacggaagc ctacttgtca gaaattattt ctgcacgaat caagcacatc cttgaacaaa
    26461 tcaagcaaga attagataga aggcgtctat tggacctccc tggtggtatt gtcttaatcg
    26521 gtgggaatgc cattttacca ggtatggttg agcttgctca ggaagtcttt ggcgtccgtg
    26581 tcaagcttta tgttccaaat caagttggta tccgtaatcc agcctttgcg catgtgatta
    26641 gtttatcaga atttgcgggt caattaacag aagttaatct tttggctcag ggagcgataa
    26701 aaggtgagaa tgacttaagt catcagccaa ttagttttgg tgggatgctg caaaaaacag
    26761 ctcagtttgt acaatcaacg cctgttcaac cagctcctgc tccagaagta gagccggtgg
    26821 cgcctacaga accaatggcg gatttccaac aagcttcaca aaataaaccg aaattagcag
    26881 atcgtttccg tggcttgatc ggaagcatgt ttgacgaata aagaggaaaa ataaattatg
    26941 acattttcat ttgatacagc tgctgctcaa ggggcagtga ttaaagtaat tggtgtcggt
    27001 ggaggtggtg gcaatgccat caaccgtatg gtcgacgaag gtgttacagg cgtagaattt
    27061 atcgcagcaa acacagatgt acaagcattg agtagtacaa aagctgagac tgttattcag
    27121 ttgggaccta aattgactcg tggtttgggt gcaggaggtc aacctgaggt tggtcgtaaa
    27181 gccgctgaag aaagcgaaga aacactgacg gaagctatta gtggtgccga tatggtcttc
    27241 atcactgctg gtatgggagg aggctctgga actggagctg ctcctgttat tgctcgtatc
    27301 gccaaagatt taggtgcgct tacagttggt gttgtaacac gtccctttgg ttttgaagga
    27361 agtaagcgtg gacaatttgc tgtagaagga atcaatcaac ttcgtgagca tgtagacact
    27421 ctattgatta tctcaaacaa caatttgctt gaaattgttg ataagaaaac accgcttttg
    27481 gaggctctta gcgaagcgga taacgttctt cgtcaaggtg ttcaagggat taccgatttg
    27541 attaccaatc caggattgat taaccttgac tttgccgatg tgaaaacggt aatggcaaac
    27601 aaagggaatg ctcttatggg tattggtatc ggtagtggag aagaacgtgt ggtagaagcg
    27661 gcacgtaagg caatctattc accacttctt gaaacaacta ttgacggtgc tgaggatgtt
    27721 atcgtcaacg ttactggtgg tcttgactta accttgattg aggcagaaga ggcttcacaa
    27781 attgtgaacc aggcagcagg tcaaggagtg aacatctggc tcggtacttc aattgatgaa
    27841 agtatgcgtg atgaaattcg tgtaacagtt gtcgcaacgg gtgttcgtca agaccgcgta
    27901 gaaaaggttg tggctccaca agctagatct gctactaact accgtgagac agtgaaacca
    27961 gctcattcac atggctttga tcgtcatttt gatatggcag aaacagctga attgccaaaa
    28021 caaaatccac gtcgtttgga accaactcag gcatctgctt ttggtgattg ggatcttcgc
    28081 cgtgaatcga ttgttcgtac aacagattca gtcgtttctc cagtcgagcg ctttgaagcc
    28141 ccaatttcac aagatgaaga tgaattggat acacctccat ttttcaaaaa tcgttaagta
    28201 aatgaatgta aaagaaaata cagaacttgt ttttcgagaa gttgcagagg ctagtctgag
    28261 tgctaatcga gagagtggtt cggtctctgt cattgcagtt accaagtatg tagatgtacc
    28321 gacagcggaa gccttgcttc cgctaggtgt tcatcatatc ggtgaaaatc gtgtagataa
    28381 gtttctggaa aaatatgaag ctttaaaaga tcgagatgtg acttggcatt tgattggtac
    28441 cttgcaaaga cgtaaggtga aagatgtcat tcaatacgtt gattatttcc atgcattgga
    28501 ctcagtaaag ctagcagggg aaattcaaaa aagaagtgac cgagtcatca agtgtttcct
    28561 tcaagtaaat atttctaaag aagaaagcaa acacggtttt tcgagagagg aactgctgga
    28621 aatcttgcca gagttagccg gactagataa gattgaatat gttggtttaa tgacgatggc
    28681 accttttgag gctagcagtg agcagttgaa agagattttc aaggcggccc aagatttaca
    28741 aagagaaatt caagagaaac aaattccaaa tatacctatg accgagttaa gtatgggaat
    28801 gagtcgtgat tataaagaag cgattcaatt cggttccact tttgttcgta taggtacatc
    28861 attttttaag taggagagaa ccatgtcttt aaaagataga ttcgatagat ttatagatta
    28921 ttttacggag gatgaggatt caagtctccc ttatgaaaaa agagatgagc ctgtgtttac
    28981 ttcagtaaat tcttcacagg aaccggctct cccaatgaat caaccttcac agtcggctgg
    29041 tacaaaagag aacaatatca ccagacttca tgcaagacaa caggaattgg caaatcagag
    29101 tcagcgtgca acggataagg tcattataga tgttcgttat cctagaaaat atgaggatgc
    29161 aacagaaatt gttgatttat tggcaggaaa cgaaagtatc ttgattgatt ttcagtatat
    29221 gacagaggtg caggctcgtc gttgtttgga ctatttggat ggagcttgtc atgttttagc
    29281 tggaaatttg aaaaaggtag cttctaccat gtatttgttg acaccagtga acgttattgt
    29341 aaatgttgaa gatatccgtt taccagatga agatcaacag ggtgagttcg gttttgatat
    29401 gaagcgaaat agagtacgat aatgattttt ttaattcgta tgatttataa tgcagtggat
    29461 atttactccc tgattttgat agccttcgct gtcatgtctt ggtttccagg tgcctacgaa
    29521 tccagtttag gtcgttggat tgtagcgttg gtgaaaccag tgcttgctcc cttgcaacgc
    29581 ctgcctttac agatagcggg tcttgattta tctgtttggg ttgcgattgt tttggttcga
    29641 tttttaggag aaaacttagt gcgttttctg gcgatgatag gatgaataaa gggatttatc
    29701 agcatttctc catagaagat cgtccatttc ttgacaaggg aatggaatgg ataaagaagg
    29761 tagaagatag ctatgctcct tttttaactc cttttatcaa tcctcatcag gagaagctat
    29821 taaagatttt ggcaaaaacc tatggtcttg cttgtagcag tagtggggaa ttcgtctcga
    29881 gtgagtatgt tcgagtttta ttatacccag attatttcca accagagttt tcagattttg
    29941 aaatatctct tcaggaaatt gtgtattcca ataaatttga acatttaacg catgctaaga
    30001 ttttagggac agtcatcaat caattaggga ttgaacggaa actttttgga gatatcctag
    30061 tagatgaaga acgggcgcag attatgatta atcagcagtt tcttcttctc tttcaagatg
    30121 gactaaagaa aattggtcgt atacctgttt cgctggagga acgtcctttc accgagaaaa
    30181 tagataagct agaacagtat cgagaactgg atttatctgt gtctagtttt cgattagatg
    30241 ttcttttatc aaatgttttg aaactatcta ggaatcaagc aaaccagttg attgaaaaga
    30301 aacttgtcca agtaaattat catgtggtag acaaatcaga ttacactgtt caagttggag
    30361 acttgattag tgtgagaaaa tttggtcgct tgagattact tcaagataag ggacaaacga
    30421 aaaaagagaa gaaaaaaata accgtccagt tattattaag taagtgagga atagaatgcc
    30481 aattacatca ttagaaataa aggacaagac ttttggaact cgattcagag gttttgatcc
    30541 agaagaagtc gatgaatttt tagatattgt ggttcgtgat tacgaagatc ttgtgcgtgc
    30601 gaatcatgat aaaaatttgc gtattaagag tttagaagag cgtttgtctt actttgatga
    30661 aataaaagat tcattgagcc agtctgtatt gattgctcag gatacagctg agagagtgaa
    30721 acaggcggcg catgaacgtt caaacaatat cattcatcaa gcagagcaag atgcgcaacg
    30781 cttgttggaa gaagctaaat ataaggcaaa cgagattctt cgtcaagcaa ctgataatgc
    30841 taagaaagtc gctgttgaaa cagaagaatt gaagaacaag agccgtgtct tccaccaacg
    30901 tctcaaatct acaattgaga gtcagttggc tattgttgaa tcttcagatt gggaagatat
    30961 tctccgtcca acagctactt atcttcaaac cagtgatgaa gcctttaaag aagtggttag
    31021 cgaagtactt ggagaaccga ttccagctcc aattgaagaa gaaccaattg atatgacacg
    31081 tcagttctct caagcagaaa tggcagaatt acaagctcgt attgaggtag ccgataaaga
    31141 attgtctgaa tttgaagctc agattaaaca ggaagtggaa gctccaactc ctgtagtgag
    31201 tcctcaagtt gaagaagagc ctctgctcat ccagttggcc caatgtatga agaaccagaa
    31261 gtagctccaa tgcatccgac aggtccaaca ccagctacag aaactgttga ttcaataccg
    31321 ggatttgaag caccgcaaga atctgttaca attttataag aaatattctg agaacaatat
    31381 cttatcctta tatttccagc gagcaggaga tggtgtgagt cctgcattcc ctatcgataa
    31441 gattatcctc tcaaaaactc aagtctgaag ctagtaagat ttgacgtttc ccacgttacg
    31501 ggataagagg gagaaagact aaatcttttt ccgaataaag gtggtaccac gattttcgtc
    31561 ctttttggaa gtcgtggttt ttaatttgtt attatttata aaggagatac catgaaactc
    31621 aaagacaccc ttaatcttgg gaaaactgaa ttcccaatgc gtgcaggcct tcctaccaaa
    31681 gagccagttt ggcaaaagga atgggaagat gcaaaacttt atcaacgtcg tcaagaattg
    31741 aaccaaggaa aacctcattt caccttgcat gatggccctc catacgctaa cggaaatatc
    31801 cacgttggac atgctatgaa caagatttca aaagatatca ttgttcgttc taagtctatg
    31861 tcaggatttt acgcaccatt tattcctggt tgggatactc atggtctgcc aatcgagcaa
    31921 gtattgtcaa aacaaggtgt caaacgtaaa gaaatggact tggttgagta cttgaaactt
    31981 tgccgtgagt acgctctttc tcaagtagat aaacaacgtg aagattttaa acgtttgggt
    32041 gtttctggtg actgggaaaa tccatatgtg accttgactc ctgactatga agcagctcaa
    32101 attcgtgtat ttggtgagat ggctaataag ggttatatct accgtggtgc caagccagtt
    32161 tactggtcat ggtcatctga gtcagccctt gctgaagcag agattgaata ccatgacttg
    32221 gtttcaactt ccctttacta tgccaacaag gtaaaagatg gcaaaggagt tctagataca
    32281 gatacttata tcgttgtctg gacaacgact ccatttacca tcacagcttc tcgtggtttg
    32341 acggttggtg cagatattga ttacgttttg gttcaacctg ctggtgaagc tcgtaagttt
    32401 gtcgttgctg ctgaattatt gactagcttg tctgagaaat ttggctgggc tgatgttcaa
    32461 gttttggaaa cttaccgtgg ccaagaactc aaccacatcg taacagaaca cccatgggat
    32521 acagctgtag aagagttggt aattcttggt gaccacgtta cgactgactc tggtacaggt
    32581 attgtccata cagcccctgg ttttggtgag gacgattaca atgttggtat tgctaataat
    32641 cttgaagtcg cagtgactgt tgatgaacgt ggtatcatga tgaagaatgc tggtcctgaa
    32701 tttgaaggtc aattctatga aaaggtagtt ccaactgtta ttgaaaaact tggtaacctc
    32761 cttcttgccc aagaagaaat ctctcactca tatccatttg actggcgtac taagaaacca
    32821 atcatctggc gtgcagttcc acaatggttt gcctcagttt ctaaattccg tcaagaaatc
    32881 ttggacgaaa ttgaaaaagt gaaattccac tcagaatggg gtaaagtccg tctttacaat
    32941 atgatccgtg accgtggtga ctgggttatc tctcgtcaac gtgcttgggg tgttccactt
    33001 cctatcttct acgctgaaga tggtacagct atcatggtag ctgaaactat tgaacacgta
    33061 gctcaacttt ttgaagaaca tggttcaagc atttggtggg aacgtgatgc caaagacctc
    33121 ttgccagaag gatttactca tccaggttca ccaaacggcg agttcaaaaa agaaactgat
    33181 atcatggacg tttggtttga ctcaggttca tcatggaatg gagtggtggt aaaccgtcct
    33241 gaattgactt acccagccga cctttaccta gaaggttctg accaataccg tggttggttt
    33301 aactcatcac ttatcacatc tgttgccaac catggcgtag caccttacaa acaaatcttg
    33361 tcacaaggtt ttgcccttga tggtaaaggt gagaagatgt ctaaatctct tggaaatacc
    33421 attgctccaa gcgatgttga aaaacaattc ggtgctgaaa tcttgcgtct ctgggtaaca
    33481 agtgttgact caagcaatga cgtgcgtatc tctatggata ttttgagcca agtttctgaa
    33541 acttaccgta agattcgtaa cactcttcgt ttcttgattg ccaatacatc tgactttaac
    33601 ccagctcaag atacagtcgc ttacgatgag cttcgttcag ttgataagta catgacgatt
    33661 cgctttaacc agcttgtcaa gaccattcgt gatgcctatg cagactttga attcttgacg
    33721 atctacaagg ccttggtgaa ctttatcaac gttgacttgt cagccttcta ccttgatttt
    33781 gccaaagatg ttgtttacat tgaaggtgcc aaatcactgg aacgccgtca aatgcagact
    33841 gtcttctatg acattcttgt caaaatcacc aaactcttga caccaatcct tcctcacact
    33901 gcggaagaaa tttggtcata tcttgagttt gaaacagaag acttcgtcca attgtcagaa
    33961 ttaccagagg ctcaaacttt tgctaaccaa gaagaaatct tggatacatg ggcagccttc
    34021 atggacttcc gtggacaagc acaaaaagcc ttggaagaag ctcgtaatgc aaaagttatc
    34081 ggtaaatcac ttgaagcaca cttgacagtt tatccaaatg aagtggtgaa aactctactc
    34141 gaagcagtaa acagcaatgt agctcaactt ttgatcgtgt cagacttgac catcgcagaa
    34201 ggaccagctc cggaagctgc ccttagcttc gaagatgtag ccttcacagt tgaacgtgct
    34261 gctggtgaag tatgtgaccg ttgccgtcgt atcgacccaa caacagcaga acgcagctac
    34321 caggcagtca tctgtgatca ttgtgcaagc atcgtagaag aaaactttgc ggacgctgtc
    34381 gcagaaggat ttgaagagaa ataagattga aaagtctagg caaaattcaa tttgagaaga
    34441 aaagacaact aattttatag tctattaaac gcattgtatc acgtttttga acacctgata
    34501 tgatgcgttt tttatttatt ttaaaaattt gcgaggtatg actttttata ctcaacaaga
    34561 atcaaagaga aacttagcaa gctaacagta gtaagataaa ataggaattt gatattaggg
    34621 ataagattgg t
//
LOCUS       DAVKQL010000018        34435 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVKQL010000018 DAVKQL010000000
VERSION     DAVKQL010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34435
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.018"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(777..1646)
                     /locus_tag="VW561_000784"
     CDS             complement(777..1646)
                     /locus_tag="VW561_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU9943071.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTENPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIVA
                     AIAAVAVFKFLYL"
     gene            complement(1870..2391)
                     /locus_tag="VW561_000785"
     CDS             complement(1870..2391)
                     /locus_tag="VW561_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9943072.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVVSGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPDPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAS
                     LGLGFFKKKEDEK"
     gene            complement(2349..3455)
                     /locus_tag="VW561_000786"
     CDS             complement(2349..3455)
                     /locus_tag="VW561_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEU9943073.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(3566..3820)
                     /locus_tag="VW561_000787"
     CDS             complement(3566..3820)
                     /locus_tag="VW561_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943074.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRTTNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRAYKLLKTLIPGENYLSC"
     gene            complement(4160..4693)
                     /locus_tag="VW561_000788"
     CDS             complement(4160..4693)
                     /locus_tag="VW561_000788"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEU9943075.1"
                     /translation="MTSFKEQEPEKVAEFLDILDNLKDLPIVYIDETGIDRYLYRPYA
                     GAPRGEKVYEKISGRRFERTSIVAGQVDGEFIAPMIYKKSMTSDFFVEWFKTQLLPAL
                     KTPHVIVMGNASFHPKNILDELCIQDKHFFLPLPPYSPDLNPIEQAWAILKKKVTDLL
                     REVPTIFECLERFFKTR"
     gene            complement(4680..4895)
                     /locus_tag="VW561_000789"
     CDS             complement(4680..4895)
                     /locus_tag="VW561_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9943076.1"
                     /translation="MGKERCEQGHIERKKRVVKNRKIPLEELKAFVEAHPDAFLREIA
                     AHFDCAVPSVWAALKQIKVTLKKDDEL"
     gene            complement(4862..5005)
                     /locus_tag="VW561_000790"
     CDS             complement(4862..5005)
                     /locus_tag="VW561_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tnpB"
                     /protein_id="HEU9943077.1"
                     /translation="MAYSTDFKQRALDYIKEGHSHVEAAKFFGVGVRTLFTWEKKDVN
                     KDT"
     gene            5239..6957
                     /locus_tag="VW561_000791"
     CDS             5239..6957
                     /locus_tag="VW561_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEU9943078.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDVEYLKEVKDVNI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKITNIEAE
                     INAFVK"
     gene            7068..7415
                     /locus_tag="VW561_000792"
     CDS             7068..7415
                     /locus_tag="VW561_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU9943079.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFIHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(7694..8530)
                     /locus_tag="VW561_000793"
     CDS             complement(7694..8530)
                     /locus_tag="VW561_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9943080.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGSRKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(8543..9172)
                     /locus_tag="VW561_000794"
     CDS             complement(8543..9172)
                     /locus_tag="VW561_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9943081.1"
                     /translation="MLELRNINKVFGDKQILSDFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(9182..9823)
                     /locus_tag="VW561_000795"
     CDS             complement(9182..9823)
                     /locus_tag="VW561_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9943082.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(9969..11198)
                     /locus_tag="VW561_000796"
     CDS             complement(9969..11198)
                     /locus_tag="VW561_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU9943083.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLVL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(11188..12354)
                     /locus_tag="VW561_000797"
     CDS             complement(11188..12354)
                     /locus_tag="VW561_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU9943084.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            12498..13511
                     /locus_tag="VW561_000798"
     CDS             12498..13511
                     /locus_tag="VW561_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEU9943085.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FVRMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(13556..13975)
                     /locus_tag="VW561_000799"
     CDS             complement(13556..13975)
                     /locus_tag="VW561_000799"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEU9943086.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(13986..15392)
                     /gene="atpD"
                     /locus_tag="VW561_000800"
     CDS             complement(13986..15392)
                     /gene="atpD"
                     /locus_tag="VW561_000800"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU9943087.1"
                     /translation="MSSGKIAQIIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(15478..16356)
                     /locus_tag="VW561_000801"
     CDS             complement(15478..16356)
                     /locus_tag="VW561_000801"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEU9943088.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(16372..17877)
                     /locus_tag="VW561_000802"
     CDS             complement(16372..17877)
                     /locus_tag="VW561_000802"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU9943089.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(17892..18428)
                     /locus_tag="VW561_000803"
     CDS             complement(17892..18428)
                     /locus_tag="VW561_000803"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEU9943090.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(18428..18922)
                     /gene="atpF"
                     /locus_tag="VW561_000804"
     CDS             complement(18428..18922)
                     /gene="atpF"
                     /locus_tag="VW561_000804"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEU9943091.1"
                     /translation="MHVTVGELIGNFVLITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(18936..19652)
                     /gene="atpB"
                     /locus_tag="VW561_000805"
     CDS             complement(18936..19652)
                     /gene="atpB"
                     /locus_tag="VW561_000805"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU9943092.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKIQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(19687..19887)
                     /locus_tag="VW561_000806"
     CDS             complement(19687..19887)
                     /locus_tag="VW561_000806"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEU9943093.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            complement(19962..20969)
                     /locus_tag="VW561_000807"
                     /pseudo
     CDS             complement(19962..20969)
                     /locus_tag="VW561_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(21414..21812)
                     /locus_tag="VW561_000808"
     CDS             complement(21414..21812)
                     /locus_tag="VW561_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9943094.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
     gene            complement(22140..22622)
                     /gene="greA"
                     /locus_tag="VW561_000809"
     CDS             complement(22140..22622)
                     /gene="greA"
                     /locus_tag="VW561_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEU9943095.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            complement(22693..24348)
                     /gene="mltG"
                     /locus_tag="VW561_000810"
     CDS             complement(22693..24348)
                     /gene="mltG"
                     /locus_tag="VW561_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU9943096.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     IRDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQTVGQLQGDFKESLTAEAF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            complement(24429..24923)
                     /locus_tag="VW561_000811"
     CDS             complement(24429..24923)
                     /locus_tag="VW561_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9943097.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            complement(24920..25384)
                     /locus_tag="VW561_000812"
     CDS             complement(24920..25384)
                     /locus_tag="VW561_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9943098.1"
                     /translation="MEIPIKIIQASKFDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQCLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEEILLYATSQGYSMIWFNPFYLEEQ"
     gene            complement(25394..26728)
                     /locus_tag="VW561_000813"
     CDS             complement(25394..26728)
                     /locus_tag="VW561_000813"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEU9943099.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            complement(26739..27356)
                     /locus_tag="VW561_000814"
     CDS             complement(26739..27356)
                     /locus_tag="VW561_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU9943100.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPITEEKPVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            complement(27404..30502)
                     /locus_tag="VW561_000815"
     CDS             complement(27404..30502)
                     /locus_tag="VW561_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9943101.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVSDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRICLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPRQATQVQADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            complement(30595..31761)
                     /locus_tag="VW561_000816"
     CDS             complement(30595..31761)
                     /locus_tag="VW561_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9943102.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDGLFEIDFDQLEKDLVEEE
                     VKLYILCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            complement(31769..32863)
                     /locus_tag="VW561_000817"
     CDS             complement(31769..32863)
                     /locus_tag="VW561_000817"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU9943103.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLATIESADYALATSSGMSAIVLAFSGFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQAIEG"
     gene            complement(33020..33565)
                     /locus_tag="VW561_000818"
     CDS             complement(33020..33565)
                     /locus_tag="VW561_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9943104.1"
                     /translation="MEQSSIYDFNLKEWRSKIAWVSQNNAVLSGSIRDNLCLGLNRLV
                     TDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRFLSGGQSQRLQIARTYLKDAEILI
                     FDEATANLDADSEYAIISSLYSVLKEKTVVIIAHSLSTVKDVDCIFFLEEGKITGSGT
                     HKELLENHERYARFVQEQMIE"
     gene            complement(33601..34140)
                     /locus_tag="VW561_000819"
     CDS             complement(33601..34140)
                     /locus_tag="VW561_000819"
                     /inference="COORDINATES: protein motif:HMM:NF012869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943105.1"
                     /translation="MTEKIQEHELIKTNQAEKSVQDVLDNCIERVQNNSLKSDRVTSF
                     ETPFALLFIFATIAVMLTYGGYRVSAGYISVGTLVSFLIYLFQLLNPISNIANFVTVY
                     SRSKGSSVALDNLLAVPKEKFEGGKSVSGQGLNFNHVYFGYDENRPVLKDITCSIFKG
                     QKLLLLDHLDQENQRLCVC"
     gene            complement(34149..>34435)
                     /locus_tag="VW561_000820"
     CDS             complement(34149..>34435)
                     /locus_tag="VW561_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9943106.1"
                     /translation="GLIYAEKSFFDKSQSGELTSAIVNDMSVIREFLITTFPNIILSL
                     VMVLGSIVVLFSLDWNLSLLLFITLPCMMFIILPLSNISEKYSRRLQEEI"
BASE COUNT        10086 a         7578 c         6261 g        10510 t
ORIGIN      
        1 aaaaatttaa ctaattaata gaaagtacta aatagactta tccttcaagg agttttcagc
       61 attatccatc cactcacttg ctgaaaatgt aaacagtctc tggtttatat agtaaaatga
      121 aacaagaata gagcaaatcg atcaggacag tcaaatcgat ttctaacaat gttttagaag
      181 tagatgtgta ctattctagt ttcaatctac tatacaacct caaaactgtg ttttgaacaa
      241 cctgcggcta gcttcttagt ttgccctttg attttcattg agtataaaat tgcagataaa
      301 acgataacaa tcataggact cagcagagtc aatgtaggtc gaagaatacc cccctaatcg
      361 cttattttat agcaaaatga aatagaaact gaacattttg ttgaggaagg taaatcaatt
      421 cctcacaatg tttgagaaag aagggaggac tattccaact tcaacccact ataaagcaga
      481 cttacaagat agggaaccca tctccgataa tatatagtag aatgaaaact gcacaatttg
      541 attggagaat tcaaaccaat ttctcacaat gttttagaaa caagggtcta ttatttcaac
      601 tgcaacctac tatagtccat tgaagctgga atagcccact gagacttcta aaccactgct
      661 ggagaggagt ttgtttagag tttctttcat ttccctaaaa aatcaaaaag agagaaccag
      721 tctgatcctc tcttacttat agatggtgct cactcaaatg ttaaaggagc tatttcttag
      781 agataaagga atttgaatac agctactgcc gcaattgctg ctacgatagg tgctactact
      841 ggtacccaag aataccacca ttttgaatcg cctttatgct caccaagaac tgattttgga
      901 aggaaagcat gaaggagacg tggtcccaag tcacgggctg ggttcaaggc aggtcctgta
      961 ggtcctccaa gtgatgttac caaagccata acgaggaatc caagtgccaa gtgagccaca
     1021 gaaagtcctg aagcagtgtg tggagccacc tgtgctttaa tagccaagtc agaaaaatca
     1081 actgtttgtc ctgcttctat tgccttttgt ttcatgaatt gaagcacttc agcaccaaag
     1141 aagtttttag tcaaaccaag agctgcaaag aacaaaacaa atgaaccaac aaactcatta
     1201 atcaaaccat tgacagttgc tgcatagcga ctttcttttg taccatggtc aatacttgaa
     1261 atagttgaga aagttcccaa gatgttattt gggttttcag ttttcaagta gaatggacgg
     1321 tatgttgcca caactaaggc ttggccaaag atagctccca agacttgcgc gataatataa
     1381 ggtaccactt gtgcccaagg gaaaagaccg ctaactgcaa gccctagagt gaaagcaggg
     1441 ttgatgtgat tcccagatac gttaccaaac atcaaggctg ggatcataac ccccatacca
     1501 taaccaacag cgatgacgat ccagccactt tggtgacctt tcgtaccttt aagttcaacg
     1561 ttggcaactg caccattccc aagaatgatc aaaatggcag ttcccaaaaa ttcagtggca
     1621 tacttcagtg cccatgtgaa atccatttgc tagattctcc ttaaaatttt ttagatattc
     1681 cttattttat caatttttac cctatttagc aactgatttc tggaaatatt tttaaaaaaa
     1741 taggattcat agtttcccag ttcaacggac ttgtaagcgt ttttattctt cacgaaagac
     1801 ctcgttctca aaaccaggaa aaaagcccac atctgtgagc ttttcctggt tcctagattc
     1861 taaaatctat tatttttcat cttctttttt cttgaagaat cctaatccta gactagctaa
     1921 caaggtcatg ataccagcac tagccaaggt agcattagct tctgtacctg tgtttggcaa
     1981 ttcctctctc ttacctgtct cataagtcgg aacttctggg tctggattca ctggagtttc
     2041 agtttttgga gtacctgggt ctggagttgg tttatctggt gttgataaac ggtcatacct
     2101 taccgttatt tctttatcac tagagtctga cgtaacttct tgtgattcaa ctgttggaat
     2161 atctggatct ttgtacttgt caatcttacc agatacaacc tcgtcccagt ttcctgttgt
     2221 ccattcaccg taggttacaa ctcccgtgac cttgttctca gtttttgtac ggcttaaggt
     2281 tacaggttga acaacatctt cttttacatt ttggttcgta actttatcaa cgtaatgaat
     2341 gatacgcgtt atagtcttcg tctcagtaga ggttgctgtt ttgggaacca ctgtttcctc
     2401 aacattctca cggtagtaat agtcaactgt tgcaccgtct tctggtacgc atttgcagga
     2461 gttgctacca aggtgtatgt tgttttcctt gtgataactc ggtcttcttt gtcctcagtt
     2521 gttgttttcc cttcaatagt ttttgattct gtggtatact cagaacctat cgctaaatca
     2581 gcttttataa cagactctgc caacttctct tggctacctt ctttatagta attggctgtt
     2641 actgtagcag tggttggcgc ttcgctttct ctataaacta aggtcactgt tctaccttcg
     2701 cttacaatat tcccagttaa acttgcagaa tttgtatctg cttctttaaa agtataatat
     2761 tttccgtcag tagtagtcat gctactgagt tttttatctg tgacattgta gctggtacca
     2821 atcagttgtt ttttattggt aatgtaggtt ccgtcacttt ctttttctcc aattccagta
     2881 tcatttttca atgatagcaa acgcccttgt tcatcaacat agcgaacttt cacattttct
     2941 gagattagtt ctgccaattc tgaggttttt ttctttttct tgatttcttc ggttattttc
     3001 cctttctctt cttcgggaat atttagtttt ggaatgattt tttcaacaac ggttcgtgat
     3061 ggttccacag tatcttggat gactgaaaag tcagctagaa ttgggagatt atactgaaca
     3121 cggtgacttt gagtgtttac tcctactctt tcattattct ctgaaaatac ttgtacggta
     3181 taagaaacaa catcttttcc taatagaaca tccccagtag agaaatagcc gccttttcct
     3241 agtttgctat ctccagagcc cacttctttc ctaatcttat cagatagttt tttaccagtc
     3301 agtacattcg ttcgcacaat ccctttgtct acccctacaa agtgggagaa ctttttgaac
     3361 tcttcagaac cagatctagc ccaaccatta ttaagggcat ttgcttttgt atttgtattc
     3421 tctctcaaaa gtttggcgat tagaattata ttcatcggca cttagagttg ctgctatatc
     3481 tgactcttga ataccaactt ccttactacc atttctagcg gcagtatatg tgtatttatt
     3541 tgtttctact tctagctgat tatccttaac atgataggta attttctcct ggtataagag
     3601 tcttcagtag tttatatgct ctgtcatcat ctttagcttt agaagcagct gagtttagcc
     3661 aagttggggt attaggagta tctttatcag cctgtttcaa gacattattc aagtcatttg
     3721 ctagcctatt ggttgtcctc actgtgaaag ttttcttcca gtctgaacca cttttattag
     3781 acttagatat gctcatgaaa tcctggtcaa cattcaacac aatatcgcct tgttttttag
     3841 catcttccgt tgcttctgct tcaaattgat catggagttt ttgttgctct ggtgtatagt
     3901 ttttttattt acacgattta atgtactatt ttttatttta gtattaagaa ctccattaat
     3961 acgattttta aactctgctt caaaaaatcc cttaatttgt tggtgcgcaa gttcaacatt
     4021 caaacctttc ttatacaagt cttcattctg aacgatttcg gctacacggt gaatggtttg
     4081 atcgtatacc gtcttctctt aaccagcagt atccttagtt ttaatgacta ctcgataagt
     4141 tcctttagaa ccgttatagt catctagttt taaaaaagcg ttccaaacat tcaaaaatag
     4201 ttggaacttc ccttaataaa tccgtcactt tctttttcaa gatagcccaa gcttgctcaa
     4261 taggattcaa atccggtgaa taaggtggta gaggtaagaa aaagtgttta tcttggatgc
     4321 agagttcatc caaaatgttc ttgggatgaa aactagcatt gcccatgaca ataacatgag
     4381 gtgtcttcaa agcaggtagg agttgcgttt tgaaccactc cacaaagaaa tcgcttgtca
     4441 tgcttttctt gtaaatcatg ggagctataa actctccgtc tacttgtcct gcaacaattg
     4501 aagttcgctc aaaacgacgt ccgctaatct tttcatagac tttctcccct ctaggagccc
     4561 ctgcataagg acgatagagg tagcggtcga ttcctgtttc gtcaatatat acgattggta
     4621 aatcttttag gttatccaaa atatcaagaa actcagcgac tttctctggc tcttgttcct
     4681 taaagctcgt catctttttt taaagtgacc ttaatctgct ttaaagctgc ccatactgaa
     4741 ggaacagcac aatcaaaatg tgccgcaatt tcccgtaaaa aagcatctgg atgagcctct
     4801 acaaaggctt tcaattcctc taaaggaatc tttcggtttt tgacgactcg ctttttcctc
     4861 tctatgtgtc cttgttcaca tctttctttt cccacgtgaa gagagttctg acgccaacac
     4921 caaaaaactt ggctgcctcg acatggctgt gcccctcttt gatgtaatct aatgctcgct
     4981 gtttaaaatc agtactatat gccatagctt tattataata tgcttgttta aaactaagca
     5041 actatatcaa tgttttgtta ttagaagact tacttttttt actgaaatcg aatttttcct
     5101 aacctcaaca tcatataacc ccaaaattca atctgacctg gtttgaagag atgttcgaag
     5161 cagataaggt tttactaaaa ctaaaaaaaa tgatataatc aaactgatta aatgttttaa
     5221 aaataaagga gaatttgaat gtcttaccaa gaaaattacc agaaatgggt tgattttgtg
     5281 gagcttcctg actaccttcg tcaagatttg gaaaatatgg acgaaaaaac taaggaagat
     5341 gccttctata caaatcttga atttggtact gcaggtatgc gtggcttggt tggtgctggt
     5401 acaaaccgta tcaacatcta cgttgttcgc caagctactg aaggattggc tcgtttgatt
     5461 gagtcaaaag gtggaaacga gaaagaacgc ggtgtagcaa ttgcctacga tagccgtcac
     5521 ttctcacctg agtttgcctt tgaatctgcg gcagttcttg ctaaacacgg catcaaatct
     5581 tacgtatttg aaagccttcg tccgactcca gaactatcat ttgcagttcg tcatctcaac
     5641 tgtttcgcag gtatcatggt cacagccagc cacaaccctg ctccatttaa cggttacaag
     5701 gtttacggtg aagacggtgg acaaatgcct ccacacgatg cggacgcttt gactacttat
     5761 atccgtgcaa tcgaaaaccc atttgcagtt gaagttgctg atgtggaaac tgaaaaagct
     5821 tctggcttga ttgaagttat cggcgaagct gttgacgtag aataccttaa agaggttaag
     5881 gacgtaaaca tcaacccagc cttgattgaa gaatttggta aagacatgaa gattgtctac
     5941 acaccacttc atggtactgg tgaaatgttg gctcgtcgtg ctcttgccca agcaggattt
     6001 gactctgttc aagttgttga agcgcaagca actgctgacc cagacttctc aactgtaaca
     6061 tctccaaacc cagaaagcca agcagccttt gcccttgctg aagaacttgg tcgtcaagtt
     6121 ggtgcagatg ttcttgtcgc aactgaccca gacgctgacc gtgttggtgt tgaagttctt
     6181 caaaaagatg gtagctacct caacctttca ggtaaccaaa tcggtgctat catggctaaa
     6241 tacatcttgg aagctcacaa aaacgctgga actcttcctg aaaatgccgc tctctgcaaa
     6301 tctatcgttt caactgactt ggtaacgaag attgctgaaa gctacggcgc aactatgttc
     6361 aacgtcttga caggtttcaa atttatcgct gaaaaaatcc aagaattcga agaaaaacac
     6421 aaccacactt acatgatggg atttgaagaa agcttcggtt acttgattaa accatttgta
     6481 cgtgataaag atgccatcca agccgttctt gtcgttgctg aacttgctgc ctactaccgt
     6541 tctcgtggtt tgacacttgc tgacggtatc gaagaaatct ataaagaata tggctactac
     6601 gcagaaaaaa caatctctgt tactctttca ggtgtcgatg gtgctgaaca aatcaaagcg
     6661 attatggcta aattccgcaa caatgctcca acagaatgga acgcaacagc tatcactgtc
     6721 gtagaagact tcaaggcaca aactgctact gttgctgacg gtactgttac aaacttgaca
     6781 actcctccaa gtgatgtgtt gaaatacaca cttgctgacg gttcatggat tgccgttcgc
     6841 ccttcaggta cagaaccaaa aatcaagttc tacattgcag ttgtagggga aaccaacgaa
     6901 gaatcacaag ctaagattac taacatcgaa gcagaaatca atgcatttgt aaaataaaga
     6961 cctataaaag atgagaccaa ccaacctcat cttttttcgt atcaaatcta agcaatttta
     7021 gaaactttat ggcatactag acttatcaat gaaagcgagg taatctcatg gaacaatttt
     7081 tagataacat caaagacctt gaagtcacta cagttgtgcg tgcgcaagaa gctcttgata
     7141 aaaaagaaac tgcaaccttc tttatcggtc gcaaaacttg cccttactgc cgtaaatttg
     7201 caggtacatt gtcaggtgtc gtagctgaaa ccaaagctca catttacttc atcaatagtg
     7261 aagaaccaag ccaactcaat gatttgcaag cattccgctc acgttatgga atcccaactg
     7321 taccaggttt tattcatatt acagatggac aaatcaacgt ccgttgcgac tcttcaatgt
     7381 cagcacaaga aatcaaagat ttcgcaggat tgtaaaaaat cagctaacaa gtcttaaaat
     7441 cacaaaaacg cataatgtaa ggtgcaaaaa ccttgatatt atgcgtttta ttgttcctaa
     7501 aacagttaca tcagcatttc attacagaac attttaattt tggaagtcaa atcaattttt
     7561 aacaatgttt tagaaatcac agtgggctat tccatattca attatccaga ttcaatcgct
     7621 attatcgttt tcctagatta caaaaaaaac tacgttttgc taatcaaaac gtagtctcag
     7681 ccactatttt atcttactgt ccttctttta cttctttggt tgctacatct tctccaaacc
     7741 atttttggct gatttcttgg aacttgccgt ccttgtaaag actagaaaaa gcttcattta
     7801 tcttcttaac caaggttgta tcttccttac gggatccaac cgcaaaagct tctgtttcta
     7861 gtccaactgt aaagacatta taatcgttta aaacaccttc tgcttctaaa taatagtttg
     7921 catagacacg gtcaatcaat agaccatcaa ttcgatcgtt tttcaaatca atcaaggctt
     7981 cattaaaggt ttggtattga ttcgcttcct tattagcgac aatattcttc aaaatttctg
     8041 gatttgcttc aaagtccgca taaccagatg aaccagcttg agctcctaat gtctttccag
     8101 tcatatcctt tgcagtcgtg ataccagatg atttcttcgt aaccaatacc tgctcattct
     8161 tcatatatga gttactgaaa gccacctttt cacggcgttc gtctgtagcg gaatagccat
     8221 tccaaatcag atcaatcgtt ccttttgtca attcagcttc tttcaaatcc caatcaatcg
     8281 gttgccaatt taccgtgatt ccgtattttt caaaaacagc tgtagctaaa tcaatatcaa
     8341 atcctgcata agaaccatct ttctgagcaa atcccattgg aacaaaagta ctatcaaatc
     8401 caatagtaat agacttgtta gactggtact ttgaccaatt atctccacta gtttcgctag
     8461 aatttttccc acaagctact aagaacaaag cagtcatcag actgactaat acaagcatcc
     8521 attttttcat ccatttttcc tcctatttag gttctacttt caataataca tctgcgatat
     8581 tttcagcaaa ctgcaaatca tgggtaacca caatctgggt catcccaagt tccctatttt
     8641 gcaagattag cttctccact tccaaacgta attctggatc cagggcagaa gttggttcat
     8701 cgtagccaat gatttctggg tcaatcatca tagcacgcgc caaagccacc cgctgctttt
     8761 gcccaccaga tagtgagaaa ggataggcct ctgcgtgtcc tcctagtcct aactgttcca
     8821 agagcccact cgccttcttc tcagcctctt cctgcttcat tcccatggtc ttcacaggcg
     8881 ataaagtcaa attttccaga actgatagat gaggaaatag ttgaaaatct tggaagacaa
     8941 atcccagtag gttgcgcttc tgcaattcat ccagctctaa aggttgtcca ttataaaaga
     9001 tttgccctga atcaatggtt tcaagacctg caagcatacg taaaagagtt gtcttacctc
     9061 caccagaagg tccaacgata gccaggattt gcttttcagg aatacttaga ctgaaatcag
     9121 acaggatttg tttgtctcca aagactttat tgatatttcg taattctaac atagctgccc
     9181 cctatctata ataactatac ttcttctcaa cttttttgga aataattgtc acaatcccaa
     9241 ttaaaatcaa gtaaatggct cctgccaaga acataggaac cagactagca tcgcggttag
     9301 cagctgttcg actagccaag ataaggtctg aaattccgag agcatagacc aaagaagtat
     9361 ccttgaccaa actcataact tcattaaaga cactaggaag aacgatcttg gtcacttggg
     9421 gcaagataat atagcgcact ctgtcaaaag ggctaaactt caagaccttg gcagcctcat
     9481 actgtcctct tggaatagtg tcaattcccc cacggaaaat ttctgcaaag taagctgcat
     9541 agttgagaac aaaggcaata atagctgcag gaaggcggtc taaacgaatc ccaatacttg
     9601 ggagcacata atagataaaa atcagttgca agagtaaggg ggttcctcgc ataacccaga
     9661 tgtaaatgtt aatcagataa tggaggggct tccaatggac ttgcaaggca aaggcaatca
     9721 aaacgcccaa cggaatcgaa aatagcaaga ccagtgcaaa gacctgtaca gtcgtgctcg
     9781 caccattcag taaactcggt aatatctcaa ataaataaga catactgcac ctcctaaaag
     9841 taattgttcc tattatagca tgaataaaca agattaccaa gtatttttgt aaaaagattc
     9901 taccatatac aaataaaaac cgtagctatc aaaagagata aactacagtt ctatatgttt
     9961 aaacattctt acaatctctc aaacagttct tgcatttgca catcatctgg aaccagtttt
    10021 aagtaagtgt gagcatggac ttttgcttct tcaaaatgtc ccaattcacg caagagatag
    10081 atatagtgtt ccagaaattc tggattgtcc ttcaaatctc ctgtcaactc ttgataatgc
    10141 tcataagcag tatccaaatc gtccatttct tgataagaac gagcaatcat ccacttggtc
    10201 aaaagatttt ctggctcctc actctgcaag tctagaatat cctcataacg ctcctgctcc
    10261 agataaatag tggctaaacg aagcaagatt tcttctgtat cctcagcgtc ttcttttgca
    10321 gtaaggagat aattttctgc accactagca tcatgcaatt cataagaaaa ttgtgaagca
    10381 gctagcaaga ggcgagtttc aaagggattt ttctctaatc cttgcttagc gatacgcagg
    10441 gcttcttgaa cttgatgttc cttatgtaaa gcctgactgt acccatactc atagccttca
    10501 aagtcaggag aaatggtatc aagctgctta aagtagaggg tggctttttg atattcttct
    10561 tgatcaaaat aaagactggc caactcaaaa gctgttaagt catcgtattc taactccagg
    10621 gctttttcta aaaactcagt agccgtttca aatttcccta actgagcata ggcaaagcca
    10681 attcgttgat aggtggaaat gcccgtttgc tcataaatcg agcgattatc taactgggca
    10741 taggcttgaa tagccgcttg gtaattttcc aactcactat ccaactctgc caaacccaat
    10801 atcaagagag aatcctctga gtaggtcaag gcctccaata atttctcacg tgccacatct
    10861 gtcaaacctt ccagctggta aaggtctgcc ttcaaaacca aagacgagac ataccagtca
    10921 ctgtcagctt ggatttcctc aagataggta aaggcttctt ctatttgacc atcctcgcta
    10981 gcaattgcag ctagattaag atgaacctct ggaaaatcct ctacaatttt caggtaaatt
    11041 tccttggcct gaggatagaa accaatccct tcaagataag ttgccaactc atacagaaga
    11101 tcacttgaat cattttctaa agctttggcg aaataatgct cagccttagt taaatcttgc
    11161 tcctccaaag cctgtaacat ctgttgacta ttgttcactc ttgacttcct caccctctaa
    11221 ttcatataga ccactgacta ccttatacca ttcgaaaatg gctgaaatga caaccttagc
    11281 agaggcataa actggaatac caagtaaaac tccccagata ccaaacatag atcctgaagt
    11341 taacaaaaca aagagaacat taatagggtg gatgtttaat tgacttccca aaatcaatgg
    11401 agagacaaaa cggccttcaa tagtttgttc tacaataaag acaatcacta ctttcaaaag
    11461 catgactgga ccagcaatca aacccaatac tagggcagga agcatggcta gaaagctacc
    11521 aagataaggg accagattta aaataccagc agtaaccccc agcgtaaccg catagcgtag
    11581 accaataatc ttgaagaaga tgataaacat tactgctaca ataatagcca ctgtcacttg
    11641 ccctcgaaca tagttggaca actgttgatt cacatctgat agaacttgtc caacaggttc
    11701 cttcaatttt cttggaatga attgggtcaa atagttacgc aagcctttcc catcacgcaa
    11761 gagataaaag agcatgaaag gaacgataat caaggcaaca atcacttgag aagccccgct
    11821 aataaaggca ctcacccagt tgactgcctg agatgaaacc ttacttgcca aaactgtagc
    11881 ctggctagaa aaattggtca aaacttgctc taattgaggt ctgaaatcat ctggcagatg
    11941 ctgggctacc aatccattaa caatcctatc tatatcttct aagtaaacag gaacgtttct
    12001 tgcaaaggtc aaaacctgac gttgcagatt tggaatggcg actgccaagc cccaaatgat
    12061 aaagagagcg atgataacaa agacaatagt gatagctata acacgattaa ccttatgctt
    12121 ctccatccaa tcaacaatag gattcaacaa ataatataac aaaccagaca aaatgactgg
    12181 caacatcaca actgctaaaa agtctaaaac aggtgaaaat agaaaactaa tcttacttaa
    12241 aataaaaaga ttcaatccca ataataaggt tactaaaaat accgtaattg ccttgttatc
    12301 taaaaaccac ttgaaaaacc aagataggct aaaatgtttc tctttttgct ccataaatac
    12361 ttcctttcta ttgttctctc attataccat ttttgaaaca aaataactct tttttaaagt
    12421 gcttacatct aattggatgt ctttaatttg cttgtttttg tgatataata gtcttatctt
    12481 tacatagagg tatagtaatg aaagaaactg tttattttgg aacttataca cgtcgtactt
    12541 ctcaagggat ttacaaggca gactttgata cagaaactgg tcagctttca aatctagaac
    12601 tttttgcagc tgagccaagt ccaacctacc ttgcctttga ccagcaccaa catttataca
    12661 ctgttggtag ccaagacgat aaggggggaa ttgcagccta tcaaactgac gggactgtgt
    12721 taaatcatgt tgttgaagaa ggagctcccc actgttatgt tgctgtcgat gaaaagcgtg
    12781 atttggttta cgcagccaac tatcacaagg gacaagtcct tgtttataaa cgccaggaag
    12841 atggtagtct tctacttagt gatatggatc aacacagtgg ccaaggtcca catgaaaatc
    12901 aagcttcccc ccatgttcac tatacagatt taacacctga ccactatcta gtgacctgcg
    12961 acttgggaac tgaccaagtc atcacctatg acctcgatca agaaggaaaa ttatctaagc
    13021 tctataccta tcacagcaag ccaggagcag gctcacgcca tatcattttc cataaccact
    13081 ataaaatcgc ttatctcatt tgtgaactca atagtactat cgaagtttta atctacgatg
    13141 gcgttggcga atttgtacgt atgcaggtta tttcaacttt accagaagct tacgaaggct
    13201 ttaatggaac cgctgctatt catctctcta aagacggtaa atacctctac gcttctaacc
    13261 gtggccatga ttctatcgca gtatatacca tccttgcgga cggtagctta gagttattag
    13321 aaatcgttcc aacgcatggt cagactccac gtgattttga tttgacaccc gaccaaaaat
    13381 ttctcattgt tgtccatcaa gactctgaca atgcaactgt ctttaaacgt aattgtgaca
    13441 atggtcgtct agcagaactc tccaacgact tccatgttcc cgaagcagtc tgcatccgtt
    13501 ttgctcctta aaacaccata aaaaatgaac ttggtatttt caagttcatt ttttcttata
    13561 gtctatttcc gacattaata cggttaatgg cacgttgcaa agcaatctta gcacgacgtt
    13621 cttggtcaat caaatgtttg tcttgtgctt cttcaattgc acgttctgca cgaagtttgg
    13681 cacgttctgc acgactgata tcgatatcac gagcacgttc tgcagagtca gcgacgattg
    13741 taatcatatc attggcaatt tcaataacgc caccgtttac tgcaatccag ttcacgtgat
    13801 ctttatcatc gatacgtttt acctttactt catcaactgc taaaaccgca atcatatttt
    13861 catgtcgtgg caagatcccc atctcaccat ccagagttcg aaccgataca tagctggcat
    13921 ggtgatcata gacgagacca tctggtgtca cgatctggac agttaactga gccataggtc
    13981 acctcttaaa atcccatttt ttcagctttt gcaatcacat cttcgataga acctacacca
    14041 cggaaggcat cttctggcaa gtggtcgtat ttaccatcaa ggatttcctt aaagccacgt
    14101 acagtttcag caactggaac ataagaacct ggctgaccag taaattgttc cgcaacattg
    14161 aagttttgtg acaagaagaa ctggatacga cgggcgcgag caaccaaggt cttttcttca
    14221 tcagaaagct catccatacc aaggatagca atgatatctt gcaattcatg gtaacgttga
    14281 aggacacgtt ttacttcagc agcaactgca tagtgctctt ctccaacgat ttcaggtgcc
    14341 aaggcacgtg agcttgaagc aagtgggtca acggctgggt agatacccaa ttgtaccaat
    14401 ttacgttcca agtttgttgt tgaatccaag tgagcgaagg ctgttgctgg cgctgggtca
    14461 gtatagtcat ccgctggcac atagatagcc tggatagagg ttacagaacc cttcttggtt
    14521 gatgtgatac gttcttgcaa ttgacccatt tccgtagcaa gtgttggttg gtaaccaacg
    14581 gctgatggca tacgacccaa aagggcagat acttctgaac cagcctgagt gaaacggaag
    14641 atattatcga taaagagaag cacgtcttgg ccttccacat cacggaagta ttcagcgatt
    14701 gtcaaaccag taagggcaac acgcatacgt gctcctggtg gctcattcat ctgaccaaag
    14761 accatggctg ttttctcgat aacgcctgat tctttcattt cccagtaaag gtcattcccc
    14821 tcacgagtac gttccccaac accagtaaat actgaaatac caccgtgctc ttgggcaatg
    14881 ttgtgaatca attcttggat taagacggtt ttaccaactc cggcaccacc gaaaagtcca
    14941 actttaccac ctttaaggta aggggcaaga aggtcaataa ccttgatccc tgtttcaagg
    15001 atttcagaag aggtagacaa ctcatcaaaa gttggagctt ttttatgaat tggctgacgc
    15061 tctgcgtctt ctgtaaaagg agcttccaaa tcaatggtat ctcccaaaac gttgaagaca
    15121 cgtcccaaag tttctttacc tactggtaca gagattggac gacctgtgtc caatacttcc
    15181 attccacgag tcaacccatc tgttgattcc atggcgatag tacgaaccat accatctcct
    15241 aactccaagg ctacttcaag gacgattttt gtttttcttt cgtcattttt gtagacgaca
    15301 agtgcattgt taatctcagg aagtttttcc cctgctgcaa acaaaacgtc tacaacggga
    15361 ccgataatct gagcaatttt acctgaactc atctccttct cctattctat ataagatact
    15421 gtcggttcct agctcaacta ggtcctaagt tcattttcat acgagctgga ctagagccta
    15481 ttctaaggca ctagcacctg ctacgatttc tgtaatttct tgtgtaatcg ccgcctgtct
    15541 ggcacggtta tactgaattg tcaaatcatt gatgactttc ttagcattat ctgtcgctgt
    15601 ttgcatggct gtcatacccg cagcattctc agctgtcttg gcatcgataa tggcaccgta
    15661 aatcatactt tctgcaaact gaggcaacaa ctgctccaga atttcttctc ggctggtttc
    15721 caattcaaaa gtcaagctgt actcttcatc cgcttcattt ggatccaagt caacaatcgg
    15781 aagcatttgt tccacacgca tttgactggt tagcgtattg acatggtggt tgtagcaaac
    15841 ataaagctca tcaaagagtt cattttggta catttcaaca gtttttgaaa taatcttacg
    15901 aacttgatca aagctaggtt ggtctgccaa gccacgtaat tcataaagtg gttgaatacc
    15961 gcgagcctta aagaaatcag ctcccatccc accgatacag atcatttcaa aacctttacc
    16021 gtctgggtgg tattcttctt tcaactccat aacagctttc aaaatagagg aattataacc
    16081 tccaaccaaa ccgcggtctg aagtgataac gatatagcct gtcttcttca cagaacggct
    16141 aatcaacatc ggattagttg aagcaccagc tccattacca tgaaggatat ctgtcaaaag
    16201 tttacgcact ttctgagcgt aaacttggaa gttgcgagca gcttcttcag agcgacctaa
    16261 cttagcagcc gataccattt gcatagcatt agtgatttga ctcgtatttt ttgttgaggc
    16321 gatttttgtt ttaatatcat ttagagacac tgccatctga cacctctatt cttattggaa
    16381 gctagattga ttgagaaact ctgtaatcgc agcatccaag actgcttctt ctggcaagtc
    16441 ttttgtatca cgaatggttt ccaaaatctc tggatgttga gcatcaaaga aggcatggaa
    16501 ctcttcctcg aaacgaacaa tatcatctac tggaacagta tccaagaaac catgtgtcaa
    16561 agcataaaga atggttactt gtttctcaac aggtaatggt ttgtgaacag gttgtttcaa
    16621 gacctcaacg gtacgacgtc cacggttcaa cttagcctgt gttgctgcgt ccaagtcaga
    16681 accaaactta gtaaaggctt ccaactcacg gtatgaagca aggtcgatac gaagtgtacc
    16741 agcaaccttc ttcatggctt tgatttgtgc agaaccacct acacgagata cagatgaacc
    16801 cgcatcgatg gctggacgaa tacctgcatt gaagaggcca tcgccaagga agatttgtcc
    16861 atcagtgata gaaatcacgt tggttgcgat ataggctgag atatctcctg cttgtgtctc
    16921 gataaatggt agggctgtaa ttgatccacc accaagttca tcagaaactt tagctgagcg
    16981 ctcaagcaaa cggctgtgga gatagaaaac atcccctggg aaggcttcac gacctggagg
    17041 acgacgaagc aagagcgata gttcacgata agctaccgct tgttttgata gatcatcgta
    17101 tacaatcaaa acatgcttac cttgatacat aaattcttcc gccatagcaa ccccagtata
    17161 aggagctagg aagagcaatg gagatggttg tgaagcagag gctgtcacaa cgattgtgta
    17221 gtccaaggca ccgtactgac gaagtgtttc tacttgcgta cgaactgttg attctttttg
    17281 tccaatcgcg acgtagatac agatcatatc ttgatctttt tggttcaaga ttgtatcaat
    17341 cgcaatggtt gttttccctg tctgacggtc accgataatc aactcacgtt gaccacgacc
    17401 aatcggtaca agggcgtcaa tagctttcaa accagtttgc aatggttctg aaacagactt
    17461 acgttgcata acaccaggag ctggtgcttc tactggacga gttttatcag tgtggatttc
    17521 tccaagaccg tcaactggac gaccaagcgg atccacaaca cgaccaatca gactttcacc
    17581 tacagggact tccatgattt tccctgtacg gcggattgta tcgccttcac ggatatctgt
    17641 aaagtcacct aggatgataa taccaacgtc tgttgactcc aagttttgag ccataccata
    17701 agagccgttt tcaaaattca acaactctcc actcatgaca ttttcaaggc catgagcacg
    17761 cgcgataccg tccccgatat aggttacaac acctgtttca gtcacatcaa aattgggttt
    17821 gaaattttca atttgttgct taattaaagc gctgatttct tgtgcgttaa ttgccaaaag
    17881 aacaccactt tctatttcaa attttcttta acaactttaa gttgttgttt aatactcaca
    17941 tcaattgtct tgtgattggc aaaaatgaca aaaccaccaa tgagactttc atcgatttgt
    18001 tcttttacac tccttacttt cagagacatt tttttctcaa tcaaagggag caaacgagtc
    18061 ttctgttcat cagttagagg atgagcagac gtaatcgtca cttcaaatcg atttgtttct
    18121 ttttcaagtc ggttcaagca atctacaagc acatcataaa aaagatttgc tctgtgattg
    18181 taggccagaa cctggataaa gttttgtaat aaaggcgaca cagaatcttg gaaaaaagca
    18241 attgtttttt ccttatccga ctcgtctact gccacttgtt ttaaaaaaga aggcagacct
    18301 gttttttcaa caacttgctt gatttgagtc aagtctgaaa agatacggtc ttcttctcct
    18361 ttttcaagta ccaattggac aaaaggcatg ctgtattttt caattacctt tactgttttc
    18421 ttgtccatta agcttctcct agctgatcga tatactgatc aatgagtgct ttatgggcat
    18481 gactgtcaag gttttgtgag atgattttac cagctaagct gatggtcaaa tctgcgacct
    18541 cacccttaac actctgtaaa gcttctactt tattttgagc aatttcttga ttggcttttt
    18601 cttttaagtg tcctgcttct agtttagcat ctgctaagat attagcctta ctttgctcag
    18661 ctgtttcctt tgcattttca atgattgtct tagcttcttt acggctacca gccaattcat
    18721 cttcgcgttt ttgagccaat acttctgctt tttgacgggc ttcttcagct ctgtcaatat
    18781 ctgaagcaat tttttcagct ctttcttcga aaatgcctgt aatattagac catgcaaatt
    18841 ttttaatcaa gactagcaaa agaataaaag agccagtgat taaaacaaaa ttaccaatta
    18901 attcacctac tgttacgtgc atcagttact cctttctatt cctcttcaat attaatctta
    18961 ttccctaaat acacagatgt caaaagagta aaaacataag cttggatgca ggaaataaag
    19021 acagaaaatg cagtccaagc caaattagct ccaaaggcta ctggatacca ataaatagct
    19081 tggtgggaaa gaagaagtaa caaactcgtc atgacctctc ctgcaaagat attcccaaaa
    19141 atccgcaaag ccaaagataa gaagtttgta aattcttcca agatattcat cggtatgaca
    19201 aaaacaggac tcataaaaga ttttatactt tttttaaatc cacgacgacg aacgctttct
    19261 atatgtgtca acaaaatgac aagaaaagat aaggttaagt catactgtaa attagcggtt
    19321 ggcgaactcc accagttagt cccatcgatc gtttgaatct ttgtcattaa gccaaggtta
    19381 ttggcaatca ccatgaaaag gaataaacaa aggaaaaaga gtgagtaatc tttcatgtag
    19441 cgcgaaccaa tgttaggttc tgtaaatcca ataacaaagt catagacata ctcaagtaca
    19501 ttttgctttc ctttgggttt caaggtcata ttgcggcttg cccaataaat aaagacaaaa
    19561 ataactccca caatcaacaa agtcatggct aacatagtca gattgaagat aacaggacca
    19621 atagagatga ttggattaat actttcttcc atctaaaatc ctcccttttc ttctcgttcc
    19681 atgtatttat ttgataataa atgagaagac aagagttaca aagaaagttc cttcaataaa
    19741 ggcaacacct aaaaacatca aactacgaaa ctcagaaagc atatctggtt ggcgtgctac
    19801 tgatttaaac agtccattca tcaataaacc ttcaccgaca gatacgccca tacaggcaat
    19861 acataagcct aaaaatgtta aattcataac aaattctcct ttaaaataaa atttacttac
    19921 ttagtgtagt cctaaaattt aaatttgtca acttttaacc gctattggaa gcgtttaata
    19981 aaaattattt ccattttgct aaatttcaag ttcaagttag ccatccagaa gtcttctctg
    20041 ggtgacttgt agtccaagca ttttttagga tagttgttaa tccacttttc gatgaatgcg
    20101 acttctttgg gagtcatttt cttggttccc ttaggtaacc atctacgaat gagcctgttg
    20161 tgattctcat tagttcccct ttcccaagag gcatagggat gtgcataata aatgtgctcc
    20221 tcagaaaata cattagacaa gcgattgaat tccgttccat tatctgccgt gatggaaaga
    20281 atcttgtgtt gttttaagat gagttttaga gcctgattga ccacctcagc acttttattt
    20341 ggaatcaatc ggatgatctg atgtctactt tttcgatccg tcaagacaag caagcagtag
    20401 tttttcgctc tcgtaagtag aaccgtatca atctcataat gcccattctc caagcgaaaa
    20461 ttgatagctt caggccgctg ttcgatggat tgaccagcag gtttaaagtt ggtgctggcc
    20521 tgtttcttaa gcgcttttcc ttttctaggg taaagcagat cctgcttgct ttaccccaat
    20581 tttccatgat gaatccaata gtaaatggtt gaaattccca cgttaacccc tttagccata
    20641 accatcattt caggcaaaaa tttttggtta tgatagtgga gaatcttttc ctttagttcc
    20701 ttggtcacgc ttgatctctt gaccgagtgc ttgcgattat tttcataaga ctgttgagca
    20761 tagtcggcag aataaacctc tttgaagcgc ccttttccaa gacattttcg gactgtccta
    20821 cgcttgattt tagtgtggat agtttgagga gcttttccaa gtagagaggc aatttctcta
    20881 tttgattttc cttctttttt ccatctttcg attaagcgac ggctatcgat tgtcaaatgt
    20941 ttaccttttg tagtataatt gtcttgcatt tctgtgcctt tcttgtgttt gtggttgaac
    21001 aacaagtata acacagaggt gttttcttat gcctacaaga gctttcatta tttccatttt
    21061 cttttggatt tcactctatt ctgaaaaact tgtgtatatt tactgaagct agcaagtctt
    21121 acctgtaaat ttaatgaaag caacacaaaa tccgagaggg gaatctcgga ttaatagata
    21181 gagagttttt agtttaaata aattgtttaa aatatcaaca acatcacttc ttttcttaac
    21241 ctgataagtc ttgattccta attttggggc tacgattata ttgtcctcaa tatcgtctag
    21301 aaagacacaa tttctaggtt ataactggta tttatcaata gttactcata tacatcagtc
    21361 cacctccata cttatgtgcg agcctctctt tgtattatac ctccatactc accttacaga
    21421 ttcttttggt aataatatct ttgcctaatg tagagatagt cttgcaaaga aaaaacttcc
    21481 ttgtagccat gtttctgata aaagtccggt gcctggaact ggtaagtatt gacaaaggca
    21541 aaacaacaat ttcgattctt agcttcactt tctgcctgtt gcaatagttt tgaaccgatt
    21601 ccttgccctc gcagttcctc ttttacaaac aaatactcga tttctagcca atttccaaaa
    21661 gtctctgcta tcaaacctgc caggagattg cccttttcat cttcgacata aagattaagt
    21721 ggctcacttt cagcctcttc tctttttgaa cggttataaa cacgaatcag attccctatt
    21781 tcttgcgatt tatgtgattc cttattttcc aatctaaagt atagtgaaat gaaataaaac
    21841 atgcgcaaat cgattaagga atttaatcta atttctaaca atgtcttaga aatcaaagtg
    21901 tactatttta acttcaatgc actatacatc taatactcaa taaaaatcaa agagcaaact
    21961 aggaaactag ccgcaggttg ctcaaaacac tgttttgagg ttgtagatga aactgacgaa
    22021 gtcagtaacc atacatacgg caaggcgacg ctgacgtggt ttgaagagat tttcgaagag
    22081 tagcaaaatg gaaaaaggag tgagtgaagc acatcgcctc cccactcctt tttctgtttt
    22141 taggctgttt tttcaacctt caagattttt acatcatagc taccaacagg cgtttcaatg
    22201 gttgctgtat cacctgtttt cttgccaatc aaggcctgcc caattgggct ttcatttgaa
    22261 accttacctg caaaggcatc cgcaccagct gaacctacga taatataaac ttcttcttcg
    22321 tcctcaccaa tttcttggat ggtgactgtt ttaccaatcg ctacttcgtc ctgggcaact
    22381 gcgtcgctat tgacgatttc agcatagcgg atttttgttt ctaagctaga gatttgtcct
    22441 tcgacaaagg cttgttcatc cttagctgct tcgtactcac tgttttctga aaggtcaccg
    22501 tatgaacggg caatcttaat gcgttctacc acttctggtc gacgaaccaa tttcaattct
    22561 tctaattctt tttcaagttt ttccttttcc tcaagggtca taggatatgt tttttctgcc
    22621 atttttctca actttcttct gataatattt tctaaagaaa attatgtgaa gtatcacata
    22681 attttagttt gtttagttta atttgctgtt gacatgttca gcgacattgc ggtcgtggtc
    22741 ttcttgattg ttagcatagt agaccttgcc ttctgtgaca tctgctacaa agtagaggtt
    22801 atcgctctta gtttgattga tgcttgactc aatcgcatcc agacttggac tatcgactgg
    22861 accaggcatg agacctacat ttttataaac attataaggt gaatcaatgt tggtatcaat
    22921 cgcaacatcc tcagctagac tgatattttg ccccagtttt ccttgggcat acaagattgc
    22981 aatattactt tgaagtggca tatcacgatt caaacgattg tagaatacac ctgcaatgag
    23041 cttacgatct tctgtcttgg caccttcttt ttcgaccaag gaagcaatgg tcaacaactc
    23101 attgacagtc aagtttttag atttgatagt actatagtaa ggagataggt tcttatccat
    23161 agcagctaac atctcatcaa tcaagctctc aatagttgtg ctttccttga tagagtatgt
    23221 agctgggaaa aggtatcctt ccaaacgata acgcgcaccg ctgtctttta caggcaaact
    23281 ttccagtaaa gtaggatatt tcgctactgc ttgactgata aacgtctcat cttgaacttt
    23341 agccaagaaa gcctccgctg tcaaagactc tttgaagtca ccttgcaatt gacccacagt
    23401 ttgagcaatc tgatccaagg tataaccttc tggaattgtc aaagtcgcaa gtacaggttc
    23461 ttgcggttca tctgttccac ctttttgcaa ctctttgagt aagtcttctg tactcatact
    23521 cttttgcaaa ttgtagtaac ctgctttcaa gtcggtataa tttttatact tggcataaaa
    23581 actaaaaatc agaccatgct ttaccaaacc agctttttca agcgtcgtac cgatttcttg
    23641 aacgtttgaa ccttctggaa ttccaaccgt cacatatttc ttagaattag catcgatagg
    23701 taataaggaa tctagcacgt actggtaacc aaagtaacca cctgctgaga gcaaggcaag
    23761 gaatacaagg aatgaaatca caaaagcttt cacatgtgac ttcttctctt tcttaggctt
    23821 aacgggtttt gctccttctc tacggctacg acgtgatgta tctctgatga tatccactgt
    23881 ttctgtagca ggcgtaggtg tttctggacc tgcttgttct gcttttttct ctgccgtcgt
    23941 ataggaaaca gctacccttg ttggggtttc attgtattct ctttcaagtt tcttaggtct
    24001 aacaggacct ggacctggtc ttgatccact ttcttccgct ggagaagaag gtacatcttg
    24061 acttggatga cttggaacac caggagtttc tctttgaatc tcatctgctg gagaagctgg
    24121 tacaccttga cttgggtgag taggcacggt aggagctttt ctcataatct cctctaccgt
    24181 tgacaaggaa tcagccatga gttcttcagc tgaaggttca tttgcaggag tgcgaactac
    24241 tgcctcatct tctttcagaa cttcatcata gccttttact ttttctaaat ctctcagaat
    24301 ctgctcttta aagcttaatt tctcttcttc tcttgacttt tcactcaaaa gtttttcctc
    24361 cttgttgata atccataata ttatatctta aaagtccaaa aaaagcaatc tatgatactt
    24421 ttcctaactt attttttcat ttcccagacc atatcatacc atgtttcccc tgcaaaggtt
    24481 gactgggact ggccttcgtt gacaaatcca tgtttttcat aataggcgat gagatagtca
    24541 tgacaggtta gattaatgcc atctctctca tcttcaagag caacttcttt caaggctgtc
    24601 agcaatttct gacccagtcc gagtccctgt gcctccttgg caatagacag acaggtcaca
    24661 gagatataac caccaggctc atgactatag tcttttattt cttctgtaaa agactggtct
    24721 tgcagatggc ggtgcaggcc aactggtcct tcgatataac ccatgattct tccttctttt
    24781 tcagcaacca gaaaagaggt ctgaatttct ctcaaatgtg cttcaaagac agaaggagga
    24841 atggcttctt cgaccgaaaa attttcaaat tcaagttcaa caatccgatc caaatcttct
    24901 aatcttgctt gtctgatttt cattgttcct ccagataaaa gggattaaac caaatcatac
    24961 tatagccctg gctagttgca tagagcaaaa tttcttcttc atcaacaaaa ccgttcattt
    25021 caaaatagga aagcagctca tcaggactct ccaaacgaat ccctttgtaa tccagctcaa
    25081 ctgccacctc tttcaaggct gcaagaagaa gtgttcccag gccctgtctc tgatggtcag
    25141 actcgatgac taaagaatgt acttttagac attgcggatt gtctgactgg ggacttgata
    25201 aaatatagcc taaaagttga ttttcatccc tagctagaag aaaggtatcc gcacacttac
    25261 ggatactttc ttctaaaata tgggaaagtt gctgcttttc agctggaaaa gacgaggtct
    25321 gaagtgcccc tatctcaggc aaatcaaact tgcttgcctg aatgatctta attggaattt
    25381 ccatgggaac atcctattga acattgcttg tcaagttaga caagagacgc tcaaatgagt
    25441 attcataggt ttggatgtct cctgctccca taaagacgta aacagcattg tcatggtcta
    25501 ggagtggaga aacattttca acagtaatca cttggtgttt tttgttgatt ttattggcta
    25561 ggtcttctac cttaacgtca ccatgatcta cttcacgagc cgagccataa atttgcgcta
    25621 gataaacagc atctgcttgg tttaaagcat gggcaaagtc gtccaacagg gcaatggttc
    25681 ttgtaaaggt atgcggttga aagactgcta caatttcctt gcttgggtat ttctgacgag
    25741 ccgcatccaa ggtcgcaata atttctgttg gatgatgggc aaagtcatca ataatcactg
    25801 tatcattgac aattttctca gtgaaacgac gtttaacacc ggcaaatgtt ttcaagtgct
    25861 cacgcaccaa gttcaaatca aatcctgctg tgtaaagaag accaataacg gctgtcgcat
    25921 tcatgatatt gtgacgacca aaggttggaa tgtggaattg ccccaagttt tgtccacgga
    25981 aatgaacggt gaaggttgaa ccagttgttg aacgaagaag atcactagct acaaagtcat
    26041 tgccttcagc ttcaaaacca taataataaa ttggtgcatc agacgtaatc ttacgcaatt
    26101 cagcatcttc accatagaca aaaagaccct tggtaatttg tttggcatag tcgttaaagg
    26161 cattgaaaac atcctcgaga cttgtgaaat aatctggatg gtcaaagtca atgttggtga
    26221 taatagagta ttctgggtgg taaggcatga agtgacgctc atattcgtca gattcaaaga
    26281 caaaatattt ggcattagcc gaaccacgac ctgtcccatc tccaatcaag aagctggtat
    26341 ctgtaatgtg agacaagaca tgagacaaca tacctgtcgt tgaagttttt ccatgtgctc
    26401 ctgctactcc catgctaaca aagtcacgca taaagctacc tagaaactca tggtaacgtt
    26461 tgtagctgat accattttgg tccgcatagg caatttcgac gttgttatct ggacgaaagg
    26521 catttccagc gataatttcc atatcaccgt ctagattctt ttcatcaaaa ggaagaatgg
    26581 taattcctgc ctgctcaaga ccgcgttggg taaagtagta cttttcaaca tctgatccct
    26641 gaaccttgtg ccccatctgg tgcaacatca aggccaaggc actcatccct gatcccttaa
    26701 ttccgataaa atgatatgtc tttgacatgt tttctcccct attctgtcat tctggtcaga
    26761 ttcaactctt gggcaacccg acgttcttgt tctgtttgtt tacttttttt attgtagatt
    26821 tggctcttct ttagaaaatc ataattgttt ttctttggag caggtgctga cacttcttca
    26881 ttcttggtag ggatagaatg aacttcttcc gccaagatat aatgagactg ggtcaatttt
    26941 tggctatatt tgacaaattc accaggattt tccttttgga aaggagttgt cggttgattg
    27001 ccctgtctaa ctagactggg ctgagaatga cgtctcgcaa ggctgaaatc ctgagttagg
    27061 tagttagcag agcgtttctt tttcaagtcc gcacgcgctt cttcacgcgc cacctccgca
    27121 tagctctttc cttctttttt aacccctaaa ggagcctttt taggtttttc gacttgcttt
    27181 tcaatcggtt ttactggttt ttcttcagta ataggagccc attctaaata atttttatct
    27241 cgatactcac ccttgatatt actgatcaga tcagactcat catagagatt catgactggc
    27301 atttcagtca acatgacctc gtcatctgac accaatggaa atcgttcttg tttcattttc
    27361 tatttccttt caacacttca ttatagcgta ttgtcttgat ttttcaagtg ctggcttcag
    27421 aaattcccaa aatttctcta atttctgcta gggtcagact accacgtgac tctgtgccat
    27481 ccaatacttg tgacaccaga tgtttctttt gttcttggag ttcctgaatt ttttcttcaa
    27541 tggttccctt ggtcaccaag cgatagacct caaccgtttc ttcctgaccc atccgatggg
    27601 cacggccaat ggcttgcgct tccaccgcag gattccacca aaggtcaacc aagatcactg
    27661 tatctgcacc tgtcaggttc agaccgaccc caccagcctt gagggaaatc agaaaggcat
    27721 ctctttctcc ttggttaaag gccttggtca tgtcttgtct ttccttggct ggggttgaac
    27781 ccgtaatttt aaaggatgtc aggcccaagt ctggcagttc ttgttcaatt ttttccaaca
    27841 ttcccttgaa ctgagagaaa atcaagacac ggtgtccgcc gtctgccacc tgtaccagta
    27901 gatctcggag actatctagt ttgccgctgg ctccctgata atcttccata aacagggcag
    27961 gagtgtcaca tatttgacgc aagcgcatca aaccagataa aatttccaca cgacttcgct
    28021 gaaattcctg ttctgacact tgagccagat ggtctcgcat ctgttgtaac tgggcaaggt
    28081 aaatagcctt ttgctggtct tccagttcat ttttataaac cacctcaatc aagtctggca
    28141 attcagtcag aacttcttct ttcttgcgtc gcatcacgaa aggcttgata aactgagcca
    28201 ctcgctctgc tggcaatttc ataaattctt tcttgcttgg caaaagtccg ggcatgacga
    28261 tttggaaaat agaccacaac tcacccagat ggttttcaat cggagttcct gacaaggcaa
    28321 agaccgacgg caccacaaat tgtctcaagg tctgggcaat cttggtctgg gcatttttca
    28381 tgacctgagc ctcatctaag aaaaggaagt caaaggccat cccctgataa aactcactgt
    28441 cctgacggaa ggtggcatag ctagtcacat agatttgatg gctctcggca agaatctcct
    28501 cacgacttgc tttcaaacca tgaacaacag ccacatccaa ctgtggggca aatttctgaa
    28561 actcatctgc ccagttgtaa atcaaacctg acggagcgag aatcaaaacc cgactttctt
    28621 ttgtcacttg actagtcaaa aaagcaatgg tctgaagggt tttcccaagt cccatatcat
    28681 cagccaaaat cccaccaaaa ccataatgat ggagcatctg caaccagcca attccctttt
    28741 cctgataatc tcgcaagtca gcctgaacct gagttgcctg tcgaggaaag tcctctggat
    28801 gtgtcaaatc ctgggccaga ttctggaatt cttgtgaaaa agaaacacgg tctcgccctt
    28861 caaagagatg agctaaactg taggccaagg atttccgagc ctgcaaggtc ccatctttta
    28921 attcaaattg ccccagttcc tgtagatttt ggcgaatttt cttggtttct tcatcgaaaa
    28981 agtaaacttg attagacgaa tcaatataaa aatcctgatt ggcaaccaag gcctgcatgg
    29041 cttggtcgat ttcctcctgg gcaatatctt gaaaatcaaa ctggatttcc aagagacctc
    29101 ccttggaggc aatctgcacc tgaggactcg ctaggctata aagctcttct agtttatctg
    29161 ataggtcaac atgcccgagt ttttcaaaga ctggaatgat atcatgaaaa aaatgataga
    29221 cagactccgc ttttaaggcc tgacgccaag attgaaaatc ggcttcaaag cccgcagcca
    29281 aacagacttg gaaaattctt tcttctaagt ccgcgtcact tgaaaatggt aattcttcta
    29341 gctcttgtcg gctagatacc tgtctatttc cataatcaaa ctgaatttct aaacaaatcc
    29401 gattatcttc ttccctgtca aagtaaaaag agggcgcaaa agttttgatt tgtagacgtt
    29461 ctggagctga aacggtgccc atctggataa aaagagtcag acaggaggcc aatttgtctc
    29521 gatcactgct atcaaattgc aggtatttct ttccttgttg acccacaggt aacgctttaa
    29581 tttccttgag aagacgcatc tgctggtctg ttaaaaaata aacctgacct ttatggaaaa
    29641 gtactgctcc ctgataaaag acattgaccc taggactctc actgatttcc atttcaaaat
    29701 agtccgagta ttctgttact gtaaaggcaa atagattggc atcagcatgc atatcctgaa
    29761 aaagcagggt ttggtagcta tccacttgat ggtcaaattg aaaatggggc aaggccatca
    29821 gtaaattcac accctgctca aaaaaggtca gagggaaaaa gaggtgccga ccttggtttt
    29881 ggaaaaagag gtctgaaacc agcccttcct ccgttagtcc gtgcaagaaa gtcaaaagtt
    29941 cttggctggc atcatcaaag gcttcccaag aaagagactc ctcataaatc ttgccaatca
    30001 tatacgactt tctctgctcg acaatcctta aaaaaagtgg aatatcccga atgacatagt
    30061 atttttggct attgatttgg ccgattctca gagtccacaa gatatgattg gttcctgctt
    30121 ccacctgacc cacagctgat aactcatagg cgcattctga ttttggagat aaaattcgat
    30181 ccaaaaactt gccacccaag gtcaccttgg tttcaacagc ctctttttct tcatgacctt
    30241 cttccagact ccacaagatt tcctgaccac gctcatcatt tttcagaaaa tgctctagcg
    30301 ctgccaaatg cacacagtaa cccctctttt gaaaaaaatc acaggcacaa aaaaccaaat
    30361 catcctctaa actatagcgc agttcttctt ctgcaacgcg agcgtagagc cgattgttct
    30421 tttccttgat gatatcaacc ttaccagttt cataaagggc aacaccttcg atacgaattt
    30481 tccccggaat caatttagcc atattttcac ctttacctta tctttttatt ataccatatt
    30541 ttcgcctatg aaaatagcct tctaggaaga cttttctcct agaaggctgg atttttaacg
    30601 tttggcaaaa gtagccacaa tccgctgaca gacttcttgc aacagagatt tgggcatagc
    30661 tatattgatg cgggcatgga gacttccttc ctctccaaaa tccaaaccac ggttgaggat
    30721 aaccttggct tcatttctca acaactcttg caatgtttca tcagtcaggt cataagctga
    30781 aaagtcaagc caaatcaagt aggtaccttg cggtttcatg accttgattt tagtctcttt
    30841 tccaaataga tccaccacat aattgatgtg gtcttcaaag acttgcttga gttcctctag
    30901 ccaatcttta ccgtatcgat aggcagcttc tgtcgccaaa taacccaagc ctgaaatttc
    30961 atgctgatta ttggccaaca ggcgtttctg gaaagctagt ctcaacttag gattttcaat
    31021 gactgcatag gaattttttg ttccagcaat attaaatgtt ttagtggcac tgctcaagac
    31081 gatagcaaaa tttttgaagg caggattgat ggtattgaaa gactggtgtt tgtgaccaaa
    31141 gagggtcaaa tcttggtgaa tctcatccga aactaacaaa acaccgtgtt tttggcagag
    31201 ttggccaatc ttctccaaca cttctttttc ccaaacacgt ccaccaggat tgtgagggtt
    31261 gcaaagaata tagagtttaa cctcctcttc caccaaatcc ttttcaagtt ggtcaaagtc
    31321 aatctcaaac agaccatcct tttccactaa ggaattagta atcaatctac gattattcaa
    31381 cttgacactg cgagcaaagg gtgggtagac aggcgtgtta attaaaaccg cctcgccttc
    31441 ttttgtaaag gtttgaatag ctgttgagat ggctggtacc acaccctcga taaagacaag
    31501 agcctctttg tcaaagttgt aaccgtattg tgtagcttcc cacttttgaa cttccttaat
    31561 taagtcttca ctggcatagg tataaccata aaccagttgg tctgcgtaag tttgcacggc
    31621 ttggcggatt tcaggcaaga ccacaaagtc catatccgct atccaagctg gtagaacttc
    31681 actatccgtt tctgtttctt tccatttata ggtatggtgc cctaaacggt tgggcaggct
    31741 tgtaaaatca tattttccca tctttgtctt atccttctat ggcttggcgc aaatctgcaa
    31801 tcaaatctct agcatcctca atcccaatag acaaacgcaa gaggtcatct gtcaaaccat
    31861 aagaatggcg tacctctgct ggaatatcag catgagtttg agtcgttgga taagtaataa
    31921 gactttccac tccacccaaa ctttccgcaa aagagaagac cttgagactg ttcaaaatat
    31981 gaggaatgcg tgtttcatcg gctactttaa aggaaatcat gcctccacga ccagtgtaga
    32041 gaacttcctt aactgctgga gaatccttca aaaaggcaac cacttcttgg gcgttagctg
    32101 ttgagcgctc catacgaaga gacaaggtct tgagaccacg aagcaactgg tagctgtcaa
    32161 atggagacaa gactgcccct gttgtattaa gattgtaaaa aagcttctcg tatagttcta
    32221 aactattggt cacaaccact ccagccaaga catcattgtg gcctgctaga tacttggttg
    32281 ctgaatggag aacgatatct gctccatctt caatcggacg ttggtagata gggctataga
    32341 aggtattgtc caccaccact ttggcaccct tagcatgagc caattttgct agtttttcga
    32401 tatcaaattc caacatcaag ggattggttg gggtttcgat atagagaaca tccacatcct
    32461 tttctaactc ggcaatcaac tcttcttctg tattggcata ggtaaaatgg aaacgacctt
    32521 cctgctccac ttggttaaac cagcgaaaag aaccgccgta aagatcacgc actgccaaga
    32581 ccttacttcc tactggaaag ccgctaaagg ccagtacaat agctgacatc cctgagctag
    32641 tcgctaaggc atagtctgct gactcaatag tcgccaagac ttcctcagcc ttactacgag
    32701 ttggattttt agtgcgcgta tagtcaaacc cagtagatcg accaaactct ggatgctgat
    32761 aggtcgttga aaaatgaagc ggtgtcacca atgcacctgt cgcttcatct gacttaatac
    32821 ccgcctgggc caaaattgtg ttaatgtgta attccttgct catacaatac ctccaaatct
    32881 atagtaacta ttgtaccact tattttcatc aactcatttt cttcttttca agagctagtt
    32941 atagtttcaa actatataaa aagggagttt ttcctgctcc ctttaataga ctataaaatg
    33001 gtgaatctca aaagacactt cactctatca tttgctcctg cacaaaacga gcataacgct
    33061 catgattttc cagtagttcc ttatgagttc ctgagccagt gattttcccc tcctctaaga
    33121 agaaaataca atccacatct tttaccgttg acaaactatg cgctataatc acaaccgtct
    33181 tctcctttaa tacagaatag aggctactga taatcgcata ctcagaatcc gcatcaagat
    33241 tagcagtagc ttcatcaaat ataagaattt cagcatcttt taagtaggtt ctagctattt
    33301 gaagtctttg gctttgcccc cctgacaaga atcgtccgcg ttcaccaact tcagtatcta
    33361 gtccctcttt catggagcga atctcatcac ctagtgatac taagtctagc actttcatca
    33421 attcatcatc agttactaag cgattcaaac cgagacaaag attgtcacga atactgccag
    33481 ataagactgc attattttgt gaaacccaag cgattttact tctccattct tttaagttaa
    33541 aatcatatat acttgattgc tccattagaa tatctcctga aagcggttta taaaaccgct
    33601 ctaacaaacg cacaatcgtt gattttcctg atccagatgg tccaacaaaa gcaatttttg
    33661 ccccttgaaa attgaacaag taatatcctt taagacaggt cgattttcat cataaccaaa
    33721 atagacatgg ttaaaattca acccttgtcc tgataccgat tttcctccct caaatttttc
    33781 tttaggaact gcaagcaagt tatccagtgc aactgaagat cccttgctcc tagaataaac
    33841 agttacaaaa ttagctatat tactaatagg attaagtaat tgaaagaggt aaatcaaaaa
    33901 cgaaaccaag gttcccacag atatatatcc tgcgctgacc cgataacccc cataggttag
    33961 catcacagct atagtcgcaa agataaataa gagagcaaac ggggtctcaa aagaagtaac
    34021 cctatctgat ttcagtgaat tgttttgtac cctttcaata caattatcca aaacatcctg
    34081 tacacttttc tctgcttggt tagtcttaat taattcatgt tcttgaatct tttcagtcaa
    34141 ttgccctgtt aaatttcctc ctgtaaacga cgactatact tttcactgat attggaaagg
    34201 ggcaagataa taaacatcat acaaggaaga gtgatgaata aaagtagaga aagattccaa
    34261 tcaagactaa ataagactac aatggaacca agtaccataa ctaaactcag aataatattt
    34321 gggaaagtcg taattaaaaa ctcacgaatg acactcatgt cattgacaat ggcagaagtc
    34381 aactccccac tttggctctt atcaaagaag gatttctctg cataaatcaa cccct
//
LOCUS       DAVKQL010000019        34254 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVKQL010000019 DAVKQL010000000
VERSION     DAVKQL010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34254
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.019"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            519..1682
                     /locus_tag="VW561_000821"
     CDS             519..1682
                     /locus_tag="VW561_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEU9943107.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQTFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGFDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            1679..2356
                     /locus_tag="VW561_000822"
     CDS             1679..2356
                     /locus_tag="VW561_000822"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEU9943108.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQFYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            2346..3200
                     /locus_tag="VW561_000823"
     CDS             2346..3200
                     /locus_tag="VW561_000823"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEU9943109.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLVETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            3219..4286
                     /locus_tag="VW561_000824"
     CDS             3219..4286
                     /locus_tag="VW561_000824"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEU9943110.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     IAEEKGLMPAGITQSITEMCQKFGLPVDYENWKVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            4296..5462
                     /gene="aroC"
                     /locus_tag="VW561_000825"
     CDS             4296..5462
                     /gene="aroC"
                     /locus_tag="VW561_000825"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEU9943111.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            5472..6575
                     /locus_tag="VW561_000826"
     CDS             5472..6575
                     /locus_tag="VW561_000826"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEU9943112.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEVIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            6586..6924
                     /locus_tag="VW561_000827"
     CDS             6586..6924
                     /locus_tag="VW561_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEU9943113.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            7016..8299
                     /gene="aroA"
                     /locus_tag="VW561_000828"
     CDS             7016..8299
                     /gene="aroA"
                     /locus_tag="VW561_000828"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU9943114.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKECTRNHTEDMLKQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIVPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEICGALIPRLIDELPIIALLATQAQDVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVAGGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            8292..8768
                     /locus_tag="VW561_000829"
     CDS             8292..8768
                     /locus_tag="VW561_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEU9943115.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     QRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            8765..9613
                     /gene="pheA"
                     /locus_tag="VW561_000830"
     CDS             8765..9613
                     /gene="pheA"
                     /locus_tag="VW561_000830"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU9943116.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAIAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWLLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            9610..10884
                     /gene="psr"
                     /locus_tag="VW561_000831"
     CDS             9610..10884
                     /gene="psr"
                     /locus_tag="VW561_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEU9943117.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFVYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            11163..12008
                     /locus_tag="VW561_000832"
     CDS             11163..12008
                     /locus_tag="VW561_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9943118.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKNFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            11998..13101
                     /locus_tag="VW561_000833"
     CDS             11998..13101
                     /locus_tag="VW561_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9943119.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLTRRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYEIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFNVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            13103..14089
                     /locus_tag="VW561_000834"
     CDS             13103..14089
                     /locus_tag="VW561_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9943120.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAKKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGYAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            14105..15538
                     /locus_tag="VW561_000835"
     CDS             14105..15538
                     /locus_tag="VW561_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEU9943121.1"
                     /translation="MIDGKRLLFSLTIVSYTLTIVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGVLARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            15567..16601
                     /locus_tag="VW561_000836"
     CDS             15567..16601
                     /locus_tag="VW561_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU9943122.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPEDSAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            16801..17538
                     /gene="mecA"
                     /locus_tag="VW561_000837"
     CDS             16801..17538
                     /gene="mecA"
                     /locus_tag="VW561_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEU9943123.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            17689..18975
                     /locus_tag="VW561_000838"
     CDS             17689..18975
                     /locus_tag="VW561_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEU9943124.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLVAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILAPDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            18977..19846
                     /locus_tag="VW561_000839"
     CDS             18977..19846
                     /locus_tag="VW561_000839"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEU9943125.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            20096..21034
                     /gene="msrB"
                     /locus_tag="VW561_000840"
     CDS             20096..21034
                     /gene="msrB"
                     /locus_tag="VW561_000840"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU9943126.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKTSLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            21092..22816
                     /locus_tag="VW561_000841"
     CDS             21092..22816
                     /locus_tag="VW561_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9943127.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISTELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVKLFKGTIRSNLTLGLNQEVSDQKLWQALEIAQAK
                     DFVSEKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFIILDDATSALDTITESK
                     LLKAIRENFPNTSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            22818..24566
                     /locus_tag="VW561_000842"
     CDS             22818..24566
                     /locus_tag="VW561_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9943128.1"
                     /translation="MKRQTVNQTLKRLAVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFVDRQGSGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKIAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRASLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQFPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            24823..26082
                     /locus_tag="VW561_000843"
     CDS             24823..26082
                     /locus_tag="VW561_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEU9943129.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIELLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVVVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    26158..26293
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            26332..26832
                     /locus_tag="VW561_000844"
     CDS             26332..26832
                     /locus_tag="VW561_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEU9943130.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            26908..27276
                     /gene="rplL"
                     /locus_tag="VW561_000845"
     CDS             26908..27276
                     /gene="rplL"
                     /locus_tag="VW561_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEU9943131.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            27463..27633
                     /locus_tag="VW561_000846"
     CDS             27463..27633
                     /locus_tag="VW561_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943132.1"
                     /translation="MQKEGQSSRQFMKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQ"
     gene            27633..27881
                     /locus_tag="VW561_000847"
                     /pseudo
     CDS             27633..27881
                     /locus_tag="VW561_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            27895..28218
                     /locus_tag="VW561_000848"
     CDS             27895..28218
                     /locus_tag="VW561_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943133.1"
                     /translation="MTFLDDYHKKHNYPLFYESYLQNVMEFLESQDIKNGVDAFVDDH
                     QNLVFVLYGQGYRAEGKEGILTTQVTVKAYDEDKKPINFANLLDSLIVSEYQMEPNLW
                     EVSYD"
     gene            28211..28591
                     /locus_tag="VW561_000849"
     CDS             28211..28591
                     /locus_tag="VW561_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943134.1"
                     /translation="MIDLYLSKNSRRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLKLTDFIEMILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            28604..28837
                     /locus_tag="VW561_000850"
     CDS             28604..28837
                     /locus_tag="VW561_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU9943135.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRHYKMEQRMPQSPWVERIVQVLDLDYEDLFRR"
     gene            28840..29427
                     /locus_tag="VW561_000851"
     CDS             28840..29427
                     /locus_tag="VW561_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9943136.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYCASLIS
                     FLWMYAQRKQAHKVNMKLRIKWIGIGLASLLIISLCFSLIHAQESTNQANLIGLQYQV
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHPGTIIAWCLYV
                     SLRMFLGLVRYKLDLWGSMGLHLVWNLLVYSLLLF"
     gene            29629..29766
                     /locus_tag="VW561_000852"
                     /pseudo
     CDS             29629..29766
                     /locus_tag="VW561_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
     gene            30056..31459
                     /locus_tag="VW561_000853"
     CDS             30056..31459
                     /locus_tag="VW561_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase"
                     /protein_id="HEU9943137.1"
                     /translation="MNNDEWVYQYPIGKFVERQGWKIHISSEYNSSHELLQDVAKICH
                     EMRIPFKHLSTEDKFIMRNGKLVSRGFSGKFITCYPNQNELESVLQKLESALKQYNGP
                     YILSYKRWDEVPIYLRYGVFRPSRDDEKKVAIDELIVGDEVVKDERLPVFKIPKGIVL
                     PDFLNKWLDKKDKKQGDFPFIIDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLG
                     FDGTYSSERLASECKALKILNEWSETPKIYWCGKIWEHTFLGIEHMKGVPLNRWVTNN
                     FPLYEVVDKTKDYLLRVSKIVEKLIDLTNKFHSENVYHQNLHLGNILVKDEDEISIID
                     WEQAVFSNDEKVVHKVAAPGFRAWRETLPSEIDWYGIRQIAHYLYMPLVTTSDLTYNY
                     VSQTRIEGKKLFESLGYTREHIDYVESLLSYLDSKCPQIENISRKKVLKPMHEIRTIE
                     SEQDIQDFIIKAFLLVN"
     gene            31648..32268
                     /locus_tag="VW561_000854"
     CDS             31648..32268
                     /locus_tag="VW561_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9943138.1"
                     /translation="MKDVVYSITPENKLSIDFSFKSPFRVLLTGTSGSGKTTILNLIN
                     GSLKPQKGYVNLLSHGKKSSDSIPTVDQTPYIFDTTIRENVTLFQNEYFSDDQIIEVL
                     KKVNLYEELEKIDILNYQCGENGSNLSGGQKQKIALARALIRNNKVYLFDEISANLDN
                     DNSNSIHDILFNLGISFIEVSHHYDLNDKRYTDIYKLENGTLFKIK"
     gene            32283..33023
                     /locus_tag="VW561_000855"
     CDS             32283..33023
                     /locus_tag="VW561_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9943139.1"
                     /translation="MDKDYILKVKGLYHQFLLGNNKTLQVLKNVSLSASRGEFISILG
                     ISGSGKSTLLKCISSLLEPTSGEVILNGINPYKIRNAKLSSIRRNEVSFIFQAYNLIP
                     SLPVIENIALPLRLSQKKLTIKNVENLLKRMKFNAGLNDFVGTLSGGEQQKVAIARAV
                     IADSDIIFADEPTGALDSVSREVIFELLRELVGAGKCVIMVTHDIELASKTDRALILK
                     DGKIFKELHRPSGEELYKILEVQSTTEE"
     gene            33026..>34254
                     /locus_tag="VW561_000856"
     CDS             33026..>34254
                     /locus_tag="VW561_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943140.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASLLQLSF
                     LVGGQIFIISSIISFIAYLCSIFVTSNYYYFLQYFFGENILPDIQFQTSAVGCIITVV
                     LISFLAFLSGCFYTFKKIRNRKSSKIRHVLSIVKRILLLAGFSVIWLLSLQQIFQDST
                     ILAKAQIIFNIVILDIVIIYQLSPFIQSCFIKLLSIIIFRNNFMFIVSKWNLLYRKPY
                     IKSISAAITGAILLISSFQMISQNILSQFQDDSDLELKVAFIVYVGAPILIVLANIIS
                     IAFLSSHQERIEIQQFEILGTSNYQMVKIKVGEAIFLTFVTSLIAFLLNIKIMALIYY
                     SLEDILIDDMNLLGLILP"
BASE COUNT        10441 a         6009 c         7479 g        10325 t
ORIGIN      
        1 gatatttttc aatttcaaaa acgttaattc ctataatgtg tctttttcaa gtatttttga
       61 tagaacatat atttagagtt cgaaatagaa tgatgcagat aggtgaaatt ataattattg
      121 ttgcttctat aatattattt attgatagta tattgagttt gtaacaaaag gcttctctat
      181 aaaatcaaat tttccagata cctattgaag cagatttatt tcttcatctt tcttctacta
      241 cctttgtcac taaatttgac agacacatat tgctgattca acctctctac ctataatctc
      301 acagttaacc tgaggctagt ttcatagttt gttatttgat tttcgttgat aaaaagaaat
      361 gctcctccaa gacggaagag cattttttat tgtgattctt acacatttac ttcctagttt
      421 tgcaggaagt ctagtatacc attttttaaa agaagagtaa aaaagcaagg gtagtttctt
      481 ttttgagaat tgtctaaaag tttgatataa tggtagttat gaatagaata agagtcagca
      541 aaagggttga aaagaagctt gctaaggggc tagttttact agaagccagt gatcttgaga
      601 atgtcaatct taaggatcag gaagtagagg tgcagggtca ggaaggaaac tttcttggga
      661 ctgcctacct ttctcagcaa aacaagggct tgggctggtt tgtcagcaaa gacaaggtgg
      721 ccttcaatca aactttcttt gaaacgttgt ttagaaaagc caaagaaaag agaaacgcct
      781 actatcaaga tgatttgaca actgcctttc gtctctttaa tcaagaggga gatggctttg
      841 ggggtctgac agtggacctt tatggcgact acgccgtctt ttcttggtat aactcttatg
      901 tttatcagat tcgtcagact atatcagaag cctttagaca ggttttccct gaggttttag
      961 gggcttatga gaaaatccgc tttaagggtt ttgactatga atctgcccat gtttatggtc
     1021 aagaagcacc tgactttttc actgttttag aaaatggtgt cctgtatcaa gtctttatga
     1081 atgatggctt gatgacagga attttcctag accagcatga ggttcgcggt agtttagttg
     1141 acggcttggc tatgggtaaa tccttgctca atatgttttc ctacacagcg gctttttcag
     1201 tagctgcggc catgggagga gctagccaga caacttctgt tgatctagcc aaacgttcac
     1261 gagaattgtc tcaagcgcat tttcaggcaa atgggctcag cacagacgag catcgtttta
     1321 tagtcatgga tgtctttgag tatttcaaat atgccaaacg caaagacttg acctacgatg
     1381 ttattgtcct agatccgcct agctttgctc ggaataaaaa acaaactttc tctgtggcca
     1441 aggattatca caagttgatt tcccagagtc ttgagatttt aaatccggga gggattatca
     1501 ttgccagtac caatgctgcc aatgtttccc gtcagaaatt tacagaacaa attgataaag
     1561 gttttgcagg aagaagttac cagattttaa acaaatatgg tcttccagca gattttgcct
     1621 ataataaaaa agatgaaagt agtaattacc tcaaggtgat tagtatgaag gttagtaaat
     1681 gaaattaatc gtttcagtaa tgccaagaag tttagaggag gctcaggctc tggatgccac
     1741 gaggtacctg gatgccgaca tcattgaatg gcgtgccgac tatctgccta aagaagcgat
     1801 tttgcaggta gctccagcca tttttgaaaa attcgcaggc cgtgagttgg ttttcacgct
     1861 acgaactcgc tccgaaggtg gagaaatcga cctttctcca gaagagtata tccatctaat
     1921 caaggaagtt gcgcaattct atcaaccaga ctatattgat tttgagtact atagctacaa
     1981 ggatgttttt gaggaaatgc tggacttccc aaatcttgtt ttgagttacc ataatttcca
     2041 agaaacacct gagaacatga tggaaatctt gtcagagttg acgatcctaa atccaaaact
     2101 tgttaaggtt gcggtgatgg ctcacacgga gcaggatgtc ttagacttga tgaactatac
     2161 acgaggcttt aaaaccctca atcctgaaca ggaatatgtg accatttcta tgggtaaggt
     2221 gggcaaggtc tctcgtatca ctgcggatgt gactggttcg agttggtctt ttgccagtct
     2281 ggatgaggtt agtgcccctg ggcagatttc tctagctagc atgaaaaaaa tcagggagat
     2341 tttggatgaa gcttgatggc tatacacgtt tagctgcagt tgttgccaat cctattaagc
     2401 attctatttc tcccttcatc cacaatagag cctttgaggc gacagctacc aacggtgctt
     2461 atgtggcttg ggagattgaa gcgagtgact tggtagaaac agtggccaat attcgtcgct
     2521 accagatgtt tggcatcaat ctgtccatgc cctataagga gcaggtgatt ccttatttgg
     2581 ataagctgag cgatgaagcg cgcttgattg gtgcggttaa tacggttgtc aatgagaatg
     2641 gcaatttaat tggatataat acagatggca agggattttt taagtgcttg ccttctttta
     2701 caatttcagg taaaaagatg accctgctgg gtgcaggtgg tgcggctaaa tcaatcttgg
     2761 cacaggctat tttggatggc gtcagtcaga tttcggtctt tgttcgttcc gtttctatgg
     2821 aaaaaacaag accttaccta gacaagttac aggagcagac aggctttaaa gtggatttgt
     2881 gtgctttaga atatgtttct gaactgcaag caaggattgc cgagtcggat ttgctagtta
     2941 atgccaccag tgtgggcatg gatggccaat cctctcctgt tcctgaaaac atagtcttac
     3001 cagaaactct tttagttgca gatatcatat accaaccctt tgaaacaccg tttttgaaat
     3061 gggctagaag tcagggcaat ccagcagtca atggtctggg aatgttgctc tatcaagctg
     3121 cagaagcttt tcaactgtgg acaggcaagg aaatgccgac agaagagatt tggcagtctt
     3181 taacagaaaa ataccaataa agaaagggag actctcctat gaaaatcaga atcgatattc
     3241 ctcatcatcc ttatgatatt cagattgaaa aaggttgtat ggcccaggct ggtcagtggt
     3301 tgcgagaact ctggcaaccg caaaaggtag tcattgtgac agataaccat gtagcctctc
     3361 tctatgcaga gaaggtcaag ctcagcctag aagatgctgg ttttcaggta gctgtttttg
     3421 atttcttaga aggtgaagaa agaaagaatt taactactgt tcagaaagtc tatgaatttc
     3481 tagtcaagca aggtctgact cgtagcgatg gaatcgttgc tcttggtggt ggcgttgttg
     3541 gggacctggc tggctttgta gcctctacct atatgcgggg tattcacttt gttcagattc
     3601 cgactagttt gacagctcag gttgattctt ctatcggtgg aaagacaggc gttaacacac
     3661 catttgctaa aaatatggtg gggacctttg cccaaccaga tggggttctg attgatccac
     3721 ttgttcttga aaccctcgga aaaagagagt tgattgaagg gatgggtgaa gttatcaagt
     3781 atggcttgat tgaggatcca gaactgtggg ctctcttgac gggactgaat ggttctgttg
     3841 agagtatttt ggaacatgca gagaccttga ttgaacattc ttgtcaggtg aagcgcaaga
     3901 tggtggttga agatgagttg gacaatggta ttcgtcttta cctcaatttt ggccacacta
     3961 ttggccatgc catcgaagcg actgccggtt atggcaaggt catgcatgga gaggctgttg
     4021 ccatgggaat ggtacagatt tccaagattg ctgaggaaaa aggcctcatg ccagctggca
     4081 ttacccaatc tatcacagag atgtgtcaga aattcggctt gcctgttgac tatgaaaatt
     4141 ggaaagttga caagctttat caggctctta ctcatgacaa gaaagcgcgt ggtaacacct
     4201 tgaaattggt cttggtgcca gagcttggtt cagcgaccat tcacccagtt tctctggaag
     4261 agatgaaaga ctacttggta aaataaggag agtgtatgag atatttaact gcaggagaat
     4321 cacacggccc ccgtctaaca gctattattg agggaattcc agctggactt ccattgacag
     4381 ctgaggatat caatgaggat cttagacgcc gtcagggtgg ctacggtcgt ggtggtcgta
     4441 tgaagattga gagtgaccag gttgtcttta cttcgggtgt tcgccacggg aagacgacag
     4501 gggcgcctat tactatggat gtcatcaata aggaccacca gaaatggctg gacatcatgt
     4561 ctgcggagga cattgaagac cgccttaaaa gcaagcggaa aattactcat cctcgcccag
     4621 gtcatgccga tttggttggg gggattaagt accgttttga tgatttgcga aattctttgg
     4681 agcgttcatc agctcgtgaa accaccatgc gggtggcagt tggtgcagta gccaaacgcc
     4741 tcttggctga gctggatatg gagattgcca accatgtcgt ggtctttggt ggtaaggaaa
     4801 tagatgttcc tgaaaatctg acagtcgctg aaattaagca aagagctgcc cagtctgaag
     4861 tttctattgt caaccaagaa cgagaacagg aaatcaagga ctatattgac caaatcaaac
     4921 gtgatggtga taccatcggt ggggttgtgg agacagtcgt cggaggagtt ccagttggtc
     4981 ttggttccta tgtccaatgg gatagaaaat tggatgcaag attggctcaa gctgttgtct
     5041 ctatcaatgc ctttaaaggg gtggaatttg gtcttggctt tgaggctggt tatcgtaaag
     5101 gcagccaagt tatggatgaa attctctggt ctaaagaaga cggttatact cgccgtacca
     5161 ataatctagg tggttttgaa ggtggtatga ctaatgggca acccatcgtt gttcgtgggg
     5221 tcatgaaacc cattcctact ctttataaac ctcttatgag tgtggatatc gaaacccacg
     5281 aaccttacaa ggcaaccgtg gagagaagtg atccgactgc tcttccagct gcaggaatgg
     5341 tcatggaagc agttgtagca acggttctgg cgcaagaaat cctcgaaaaa ttctcatcag
     5401 ataatcttga ggaactaaaa gaagcggtag ccaaacaccg agactataca aagaactatt
     5461 aaggagttcc tatggcaaaa acaatctata tcgcaggtct tgggttgatt ggagcctcta
     5521 tggcacttgg tatcaaacgc gatcatccag attatgaaat tttaggttat aatcgtagtc
     5581 aagcttcgag agatatcgcc ttgaaagaag gtatgattga ccgtgcaacg gatgattttg
     5641 ctagttttgc tcctttggca gatgtcatta tcctcagctt gccaatcaaa caaactattg
     5701 ctttcattaa ggagttggcc aatttggatt tgcgagaagg cgttattatt tcagatgctg
     5761 gttcgaccaa gtcaaccatt gtggatgcgg cggagcagta tttggctggc aagtctgttc
     5821 gctttgtcgg ggcccatccc atggctggta gtcacaagac aggggctgct tcggcagatg
     5881 tcaatctttt tgaaaatgcc tattatatct ttacaccttc aagcctgaca agtcaggaca
     5941 cgcttaagga aatgaaggat ctgctttcag gtcttcatgc tcgttttatc gagattgatg
     6001 ccaaggagca tgatcgtgtc acttctcaga ttagccattt tcctcatatt ttggcttcta
     6061 gtctcatgga gcagactgcg gtctatgctc aagagcatga gatggcaagg cgctttgcgg
     6121 caggtggttt tcgagatatg acccgaattg cggaaagcga gccaggaatg tggacctcca
     6181 ttctcttgtc caatagcgag accattctgg atagaattga ggatttcaag gaacgtttgg
     6241 aagtgattgg tcaggccatt agtaagggag atgaagagca aatttggaac ttttttaacc
     6301 aagcgcgtga acaacgccag accatggaaa tccataagcg tggtggtgtg gatagctctt
     6361 acgacctcta tgttgacgtt cctgatgaag aagatgtcat cctgcggatt ttggaactgc
     6421 tacgtggaac ttccttggtt aacatccaca tcaacgagga aaatcgtgag gatattcacg
     6481 gaatcctaca aatttcattt aaaaatgctc aagacttgga aagagctgag catctcataa
     6541 cagaaaatac cgactataca gtcgtcatca aataaggaga agaacatgtc aaatatttac
     6601 gatagtgcaa acgaactcag tcgcggtcta cgcggattac cagaatacaa ggctgttaaa
     6661 gcagctaaag atgcgattgc agcagatgct gaggcaagca aaatctttac agaatatctt
     6721 gcctttcaag aggaaattca aaaactagca cagacaggtc aaatgccaga tgcgtccttc
     6781 caagctaaga tggaaggctt tggtaaacag attcaaggaa atagcctctt gtcagaattc
     6841 tttaccaagc aacaacaatt ggccatttac ctttctgaca ttgaaaaaat tgtttttgaa
     6901 ccagtttcag aattgctgaa ataagaaaag aacgatccta aagtcaggaa tttttaagga
     6961 atttctgact ttttatgata aaataggtaa aagtattgca agtatgaggt ccagtatgaa
     7021 actaaaaaca aacattcgcc atttacatgg tagtatccgc gtcccaggtg acaagtctat
     7081 cagccaccgt tccattatct ttggaagttt ggctgagggt gagaccaagg tttatgatat
     7141 tctgcgaggt gaagacgttc tttcgaccat gcaggttttt cgtgaccttg gtgttgaaat
     7201 tgaggataaa gatggggtta ttaccattca aggtgtaggc atggctggct taaaagcgcc
     7261 acaaaatgcc cttaatatgg gaaattctgg cacctcgatt cgcctgattt caggtgtcct
     7321 tgctggtgca gatttcgaag tagagatgtt tggagatgat agtctttcca aacgtcctat
     7381 ggaccgtgtg acccttccac tgaaaaaaat gggcgtcagc atttcagggc aaactgaacg
     7441 agacttgcct ccccttcgct taaaagggac gaaaaaccta agacctattc attatgagtt
     7501 gccaattgcc tctgcccaag tcaagtcagc cttgatgttt gcagccttac aagctaaggg
     7561 ggagtcagtt attatcgaaa aagagtgcac ccgtaatcat actgaagata tgttgaaaca
     7621 atttggtggt catttaagtg tggacggtaa gaaaatcaca gtccaagggc cacaaaaatt
     7681 gacaggacag aaggtggtcg taccaggaga tatttccagt gcagcctttt ggttagtcgc
     7741 aggtttgatt gttccaaatt ctcgtctagt gctgcagaat gtggggatca atgaaactcg
     7801 caccggtatt attgatgtca ttcgtgccat gggtggaaaa ttggaaataa ctgaaatcga
     7861 tccagtcgct aaatctgcaa ccttgattgt tgagtcttct gacttgaaag gaacagagat
     7921 ttgtggcgct ttgattccac gtttgattga tgaattgcct attattgccc tacttgcgac
     7981 ccaagcccaa gatgtaacag ttatcaagga tgctgaggag ctcaaggtca aggaaacaga
     8041 ccgtattcag gttgtggcag acgccttaaa tagtatggga gcagatatta ctcctacggc
     8101 agatgggatg attatcaaag gaaaatcagc tcttcacggt gctagagtca atacgtttgg
     8161 tgaccaccgt atcggcatga tgacagctat cgcagcccta ttggttgcag gtggagaggt
     8221 ggagcttgac cgtgcagaag ccatcaatac cagctatcct agtttctttg atgatttgga
     8281 gagcttgatt catggctaag gtgttattag ggtttatggg ggctggaaaa tcgactattg
     8341 caagaggctt ggaccctaat taccttgata tggatgctct gattgagaag cgcctaggta
     8401 tgtccattgc gaattttttc gctgaaaagg gagaagcagc ctttcgtcag gtagaatcag
     8461 aagtcctagc tgatttacta caaacagacc aagtcgtgtc aactggagga ggagtggtta
     8521 tttctcagag aaatcgtgac ttactcaaga ctaatacaga taacatctac ctgaaagcag
     8581 attttgaaac cctctaccaa cgtatcgcag ctgataagga caatcagcga ccgctttttc
     8641 taaataatag caaggaagaa ctagtagcta tttttcaaga aagacaggct tggtatgagg
     8701 aagtggctag tcgggttttg gatgtgacca agctaagccc agaggaaatt atagaggaac
     8761 taagatgaaa attgcttatc taggtcccaa aggatcattt tcacaccacg ttgtgcagac
     8821 agcttttcct catgaggaat tgcaggcttt tgccaacatt acagatgtca tcaaggctta
     8881 tgagcaagga ttagtggact attctgtggt gccagttgaa aattctattg agggtagtgt
     8941 tcatgaaacc ttggactatc tttttcatca ggctcacatc caagcagtag cagaaatcgt
     9001 tcagcctatt catcagcagt tgatggtggt tccaggccat actaagattg aaaagatttt
     9061 ttcacatcca caggccttgg ctcaaggaaa gaaattcatc gatgaacaat atccagaggc
     9121 tcaaatcgag gtaacagcta gtacagctta tgcggcccgt tttatttccg aacatccaga
     9181 tcagcccttt gcagcaattg cacctagaag ttctgctgaa gaatatggat tggaactgat
     9241 tgccgaggat attcaggaaa tggaagccaa tttcacacgt ttctggcttc taggagctga
     9301 aaagcctagt attcccttgc aagcacaaac tgaaaagatg agtttggcct tgacattacc
     9361 tgacaacctt ccaggtgcac tttataaggc cctgtcgacc tttgcttggc gaggaattga
     9421 cttgacaaaa attgaaagtc gtccactcaa gacagcactg ggtgaatact ttttcattat
     9481 cgatgtggat tataccgata aggacttggt ccactttgcc caaaaagaat tagaagcgat
     9541 tggaatccag tataaaattc tgggtgccta tcctatttat ccaatatcag accatggaaa
     9601 ggagagaaga tgagtaaaga aaatccctta agtcatcatg agcagttgcg ttatgattat
     9661 ttgctaaaaa atattcacta tctcaatgag agagaaaaaa atgagtttgt ctatttgcaa
     9721 gaaaagctaa ctcttgctag gggaaatagt agctctagct tggaacaaga aagagaagag
     9781 caggttgact taccaagcta tgcgaaccgg agtcgctcac aatccaaatc acaagcactc
     9841 tctttccctc caaaaaagaa aagacggaag ctccgtctta aacgaatttt tatggtgatt
     9901 ttttcacttt tggtctgtgt ggctttggcc atggtattca tgtttttgcg tggttaccaa
     9961 gatgctagcg caaagaaaac tgctgatgcc cgggcagctc aagtagaagt ctttaatggt
    10021 caggacacta gagatggagt taatatttta atcatgggta ctgatggtcg aatcggccag
    10081 aacagtgttg agacacgaac tgactctatt atggtattaa atgtcggggg ctcagataag
    10141 aaaatgaagc tagtcagttt catgcgtgat aatttggtct atatagatgg ttatagtcaa
    10201 gtgattaacg gtagaaaaca gacagataac aagttaaacg tagcctacga gttaggagaa
    10261 caagaggggc aaaaaggggc agaaatggtt cgccaagtct tgaaagataa ttttgacttg
    10321 gacattaagt actacgcctt ggttgatttc caggcctttg ccacagcgat tgacacgctt
    10381 ttccctgatg gggtgacgat tgatgctcaa ttttcaacat tgaatgggcg tcccctaaca
    10441 gaagctacag tcggagatga tttatacgct accgagactg agtctccaac ccaaaccatt
    10501 aaagtcggaa aacagcagat gaatggctcg accctgctca attatgctcg tttccgtgat
    10561 gatgatgagg cggattacgg ccgtacaaaa agacagcaac aagttttaac agcaatttta
    10621 gagcaaatta aagatcccac taaacttttc actggttctg aagctcttgg aaaggttttt
    10681 gctatgacct caaccaacgt accctatact ttcctcctaa caaacggttt atctgttttg
    10741 gatggagcaa aaaatggtat tgaaaaattg acgattccag aactgggtga ctgggtagat
    10801 gcctatgatg tttatggagg cttgggcttg ctggttgatc aaaacaaata tcaaaccaag
    10861 ctcgctcaaa tgggcttgag ataagataat actcaatgaa aatcaaagtg caaattagga
    10921 agctagccgc aggctgctaa aaacacagtt ttgaggttgt agataagact gacgaagtca
    10981 gttacctata cctacggcaa ggtgaagctg acgttgtttg aagagatttt cgaagagtat
    11041 aagacagaaa aatcaaagtt tgatagctat ttggatagca gtcaggcttt gattttttac
    11101 agtctgcaat agattttcac cccttgtttg tggtataatg aaacgatacg atagaaagaa
    11161 taatgaataa tatgactgat ttaaaagcaa ttcaggctcg tagtctggag atggctgaat
    11221 attttgtggc cttttgtaaa gaacatgatt tgctctgtta tctctgtggc ggaggcgcta
    11281 ttggtgccct tcgaaacaag ggatttattc cttgggacga cgacctagac ttttttatgc
    11341 ctcgtaaaga ttatgagaaa ttagcagaat tatggcctcg ttatgcagat gaacgttatt
    11401 tcttgtcaaa gagtcacaag aattttgttg atcgtaatct ttttattacc attcgtgaca
    11461 aggaaaccac ctgtatcaag ccttatcagc aggatttgga tttgccacat ggtctggcct
    11521 tggatgtttt gcctttggat tattatccga aaaatccagc tgagcggaaa aaacaggttc
    11581 gttgggcctt gatttattca ctcttttgtg cgcaaactat tccagaaaag catggtgctc
    11641 tcatgaaatg gggaagtcgc attttactgg gtttgactcc aaaatctctc cgttatcgca
    11701 tctggaaaaa agctgagaaa gaaatgacta agtatgattt ggctgattgt gatggcatta
    11761 cagaattatg ctcaggtcct ggctacatga gaaacaagta cccaatcaca tcttttgaag
    11821 acaatctttt cttgccattt gaaggaacag agatgcctat tccaatcggc tatgatgtct
    11881 atctcagaac tgcttttggg gattatatga cgcctccacc agcagacaag caggtaccgc
    11941 atcatgatgc tgtcatcgct gatatggata agtcttatac agaatacaag ggagaatatg
    12001 gtggctaaga aaaaaatctt attttttatg tggtcttttt ctcttggagg tggtgcagag
    12061 aagattctat caaccattgt ttcaaatctg gatccagaaa agtatgatat tgatattctt
    12121 gaaatggagc actttgacaa gggatatgaa tctgttccaa agcatgtacg cattttaaaa
    12181 tcccttcaag attatcgcca aaccagatgg ttacgagctt ttttgtggag aatgagaatt
    12241 tattttccaa gactgactcg tcgtttgctt gtaaaagatg attatgatgt tgaagtttct
    12301 tttaccatta tgaatccacc actgttgttc tctaaaagaa gagaagtcaa gaagatatct
    12361 tggattcatg gaagtattga agaacttctt aaggatagct ctaaaagaga atcacataga
    12421 agccagttgg atgctgcgaa tacaattgta gggatttcaa aaaagaccag caattctatc
    12481 aaggaagttt atccagatta tgcttctaaa ttacagacaa tctacaatgg atatgatttt
    12541 cagactattc tagaaaaatc tcaagagaag atcgatatcg agattgctcc tcaaagtatc
    12601 tgtactatcg gacggattga ggaaaataag ggttctgacc gtgtagtgga agtgatacga
    12661 ttattacacc aagagggaaa aaactatcat ctctatttta tcggggctgg tgatatggaa
    12721 gaggaactga aaaaacgagt caaagagtat gagattgagg actatgtaca tttccttggt
    12781 tatcaaaaaa atccttatca gtatttatct cagacgaaag ttctcttgtc tatgtctaaa
    12841 caagaaggct ttcctggagt gtatgtggag gccttgagtc tgggactccc ttttatctct
    12901 acggacgttg gaggggctga ggaattatcc caagaaggac gatttggaca aatcattgag
    12961 agcaatcaag aggcagctca ggcgattact aattacatga cttctgcctc aaactttaat
    13021 gtcgatgagg ctagccaatt cattcaacaa tttacaatta caaaacaaat cgaacaagta
    13081 gaaaaactat tagaggagta gcatggaaac tgcattaatt agtgtgattg tgccagtcta
    13141 taatgtggcg cagtacctag aaaaatcgat agcttccatt cagaagcaga cctatcaaaa
    13201 tctggaaatt attcttgttg atgatggtgc aacagatgaa agtggtcgct tgtgtgattc
    13261 aatcgctgaa caagatgaca gggtgtcagt gcttcataaa aagaacgaag gattgtcgca
    13321 agcacgaaat gatgggatga agcaggctca cggggattat ctgattttta ttgactcaga
    13381 tgattatatc catccagaaa tgattcagag cttatatgag caattagttc aagaagatgc
    13441 ggatgtttca agctgtggtg tcatgaatgt ctatgctaat gatgaaagcc cacagtcagc
    13501 caatcaggat gactattttg tctgtgattc tcaaacattt ctaaaggaat acctcatagg
    13561 tgaaaaaata cctgggacga tttgcaataa gctaatcaag agacaaattg caactgccct
    13621 atcctttcct aaggggttga tttacgaaga tgcctattac cattttgatt taatcaagtt
    13681 ggccaagaag tatgtggtta atactaaacc ctattattac tatttccata gaggggatag
    13741 tattacgacc aaaccctatg caaagaagga tttagcctat attgatatct accaaaagtt
    13801 ttataatgaa gttgtgaaaa actatcctga cttgaaagag gtcgcttttt tcagattggc
    13861 ctatgctcac ttctttattc tggataagat gttgctagat gatcagtata aacagtttga
    13921 agcctattct cagattcatc gttttttaaa aggctatgct tttgctattt ctaggaatcc
    13981 aattttccgt aaggggagaa gaattagtgc tttggcccta ttcataaata tttccttata
    14041 ccgattctta ttactgaaaa atattgaaaa atctaaaaaa ttacattaga acgggggtga
    14101 ccaagtgatt gatgggaaac gattattatt tagtttgacc atagtcagtt ataccttgac
    14161 gatagtaagt ggaattgtgt atctgtttaa taataataat gttagcttac tttctacttt
    14221 attgttcttg ttggttagta gcttaattgc ttgttggaat gatatcaagt attacttaat
    14281 ccattttatt ttctatttaa ccatttttgt atttctggta tcaagaccga ccattgatta
    14341 ttttagggat ggtgctttgg atacctatca tccaatagcc tatcgttttg cctttatagt
    14401 tgttatggtt tcgattctgg gcttgaccac aggaggcgtc ctggctcgtt acttcatagc
    14461 taggaagaaa ataaaagtag caaatatagg aaattctcta aaagaggttt atatcaagcg
    14521 gttacgcttt gtatcactag gagtttttct tctaacctat cctttctatt tcattcggtt
    14581 atttgaacgg ctcttgtatc gtttgcagac ttcctactat gcctactatg caaattttga
    14641 aagtaaactg ccttatttta cctacatttt gtctaccttt acggtctatg caatgtgtat
    14701 gtatctggca accaagccaa agaaattgca ggccacagca gtgcttgtct cctttattgc
    14761 agctaatact attcatttgg caattgggac acgaaatccc tttattttaa gtattttatt
    14821 tgcttttgtt tattacttta tgcgggagca aactgaaaaa ggaaaatgga ttgggtttaa
    14881 agaaaagtta gcgatttttg taggttctcc tattctcatg ttagcgatgg gagtacttaa
    14941 ttatgtacgg gataatgtcc aagtttccca tacaggtttc tgggatatct tacttgactt
    15001 tatctataaa caaggaacta gttttggtgt tctggctcga ggttttctat ttaacagtag
    15061 cctcccttac cgagatttcc gtaattttac ttttggtcct gttcttgatt attttgcaag
    15121 ggggagtttg ggagccattt tcggaggaaa agcctttgaa catacaacca atagtgtgga
    15181 actagctatt gatagtaata gttatgccca caatctatcc tatcttgtct tgaacaagga
    15241 atacttgaaa gggcatggta tcggaagtag ttatatcatg gagttgtata ccgactatgg
    15301 tatgattgga gtctttctgc ttagtttctt actcggcgta ttatttatag ccatgctgca
    15361 agtagcctat cgctcaagga caatcctatt tgctttatcc ctactcatct tgaataatct
    15421 attctttatg ccaagaagca gcttttcaga aagtttcttc aatttattta caatgcaatt
    15481 ctggggaatt gttcttgtga ttatatttgt agcaaaaatg cttacaaagg aaaactagta
    15541 tctactaaac aaaggagaaa aaaatcatgt ttgaacatta ttcagtagct gatttgtttg
    15601 caaatcttta taaaaaacga aaagcaaata ttttagctct catcgcttta tttgctctca
    15661 ttgctgtacc atttacaatt aaagcagtta ggaataaaaa cactgtcaaa gatacaacaa
    15721 gttattcaac ttatcttatc tataaaatca ctcctccaga agattcggcc aaaacgattt
    15781 tgaatcatca aattggtggt tatagtgatt tttatgggaa attgattgat ggtaatttga
    15841 atggagctta tcttttcaat gatgtagaac ccagtgagtt gaaaaaaatt gccagtgaat
    15901 tagacacgac ggaaacaacc ttgaaaaatt caacgaatga ctattggtgg aaaaaattga
    15961 ccgtctacta tatgattgac gatgcagggg ttggtgtgaa aattttgaca tcaagtaaag
    16021 atgccaatga cttgttagag aaaaaaattg atgggttgat tgagaaattt aaacatgctt
    16081 atgcaaatgt gaaaattgaa aaactggaaa ccatcaactc taaagaattg aacgcaaatg
    16141 gtgaaacagc gcttggctta aatgtgaaaa atctgattct tcgtttagtt gttattggag
    16201 tggtttgtgt gattttggtt gtgatgggaa atgtgttagt ttatctcttt aatccaacaa
    16261 tcaatagagt aggtgatttt tctcagtatc aaattgattt tgtaacagag attacaacaa
    16321 ttgctaacct agcagatgtt ttgtcataca aaaatactgg acaggaattg accatcgtta
    16381 gctcaaataa agctatccta gataaattga aacagagtca agaagcttta aaaggaatgc
    16441 attttgtaga tttgcaggat gtatcatctc ttttggaaag agatacagtc cttcttgttg
    16501 aagagtacgg agtgactcgt tataagaaat ttgagcaaag tcttcaaatt ctcagaaact
    16561 taaatcgttc tatcctcggt gtagcaacct ttaaattata agtattctga tgtataaggt
    16621 cttcaaaatc ggctaagagc cgattttgaa ggctttttct atctgtctgc agaaattttc
    16681 tcttggagtc tcctatagaa agaccctagg gaaaatctat ccgtatagag gagccttctt
    16741 ttgataaaat cgtaaaaatc tagtataata gatagaactg aaagtatgag gttactagca
    16801 atgaaaatga aacaaattag tgatacaact ttaaaaatca cgatgtcttt agaggatttg
    16861 atggatcgtg gaatggagat tgctgacttt ctcgttcctc aagaaaaaac agaagagttc
    16921 ttttatgcta tcttggatga gctagagatg cctgatagct ttctggatac aggcatgttg
    16981 agcttccgtg tgactccaaa acctgataag gtagatgtct ttgtgaccaa gtcaaagatt
    17041 gaccaaaatc tagattttga agacttatcg gatttgccag atatggaaga attggctcaa
    17101 atgtctccag atgaatttat caaaaccctg gaaaaaagca tcgcagacaa aaccaaggat
    17161 gatatcgaag ccattcaatc tcttgagcaa gttgaagcca aggaagaaga gcaagagcag
    17221 gctgaacaag aagctgagag taagaaagaa ccttacatct actacatcct ttcttttgct
    17281 aagttggctg acttggtagc ttttgccaag acagtgactt ttgaaatgga aacttctgaa
    17341 ctctacaaaa tgaacgagcg ctattatttg accattttag tggatattga aaatcatcca
    17401 agcccatatc cagcttggct gttggcccgt atgcgcgagt ttgcagacga tagtgatatc
    17461 agtcgctcag tcttacaaga gtatggtcaa gtcttgatga gtcacgatgc agtgctcaat
    17521 ctgcaaaaaa tcggctaatc ctttctggaa agctattttc agattttaag aagagcgaat
    17581 cgtctgattc gttttttctt tttatactga aatagtgatt tactataata ggaattttca
    17641 caaaattctg ttataatggc tatattagaa aatttcgagg agataaagat gacagttaaa
    17701 attgctttac taggatttgg taccgttgca agtggtgtgc ctttcctcct aaaggaaaat
    17761 ggaggaaaaa tcaatcaatc agcacattca gatatcaaag ttgctaaggt attggtcaag
    17821 gatgaagatg aaaaaaatcg cttgcttgta gcagggaatg actttaactt tgtaaccaat
    17881 gtggatgata ttttatcaga ccaggatatt actatcgtag tggaattgat ggggcgtatt
    17941 gagcctgcta aaacctttat cactcgtgcc ttggaagctg gaaaacacgt tgttactgct
    18001 aacaaggacc ttttagctgt ccatggcgca gaattgctag aaatcgctca agctaacaag
    18061 gtagcacttt actacgaagc agcagttgct ggtgggattc caattcttcg tactttagca
    18121 aattccttgg cttctgataa aattacgcgc gtgcttggag tagtcaacgg aacttccaac
    18181 ttcatggtga ccaagatggt ggaagaaggc tggtcttacg atgatgctct tgcggaagca
    18241 caacgtctag gatttgcaga aagcgatccg acgaatgacg tagatgggat tgatgcagcc
    18301 tacaagatgg ttattttgag ccaatttgcc tttggcatga agattgcctt tgatgatgta
    18361 gcccacaagg gaatacgcaa tatcacacca gaagacgtag ctgtagctca agagcttggt
    18421 tacgtagtga aattggttgg ttctattgag gaaacttctt caggtattgc tgcagaagtg
    18481 actccaacct tcctacctaa agcgcaccca cttgctagtg tgaatggcgt aatgaacgct
    18541 gtctttgtag aatctatcgg tattggtgag tctatgtact acggaccagg tgcgggtcaa
    18601 aaaccaactg caacaagtgt tgtagctgat attgtccgta tcgttcgtcg tttgaatgat
    18661 ggtaccattg gcaaagactt caacgaatat agccgtgact tggtcttggc aaatcctgaa
    18721 gatgtcaaag caaactacta tttctcaatc ttggctccag actcaaaagg tcaggtcttg
    18781 aagttggctg aaatcttcaa tgctcaagat atttccttta agcaaatcct tcaagatggc
    18841 aaagagggtg acaaggcgcg tgtcgttatc atcacacaca agattaataa agcccagctt
    18901 gaaaatgtct cagctgaatt gaagaaggtt tcagaattcg acctcttgaa taccttcaag
    18961 gtgctaggag aataagatga agattattgt acctgcaacc agtgccaata tcgggccagg
    19021 ttttgactcg gtcggtgtag ctgtaaccaa gtatcttcaa attgaggtct gcgaagaacg
    19081 agatgagtgg ctgattgaac accagattgg caaatggatt ccacatgatg agcgtaatct
    19141 cttgctcaaa atcgctttgc aaattgtacc agacttgcaa ccaagacgct tgaaaatgac
    19201 cagtgatgtc cctttggcgc gcggtttggg ttcttccagc tcggttatcg ttgctgggat
    19261 tgaactagcc aaccaactgg gtcaactcaa cttatcagac catgaaaaat tgcagttagc
    19321 gaccaagatt gaagggcatc ctgacaatgt ggctccagcc atttatggta atctcgttat
    19381 tgcaagttct gttgaagggc aagtctctgc tatcgtagca gactttccag agtgtgattt
    19441 tctagcttac attccaaact atgaattacg tactcgcgac agccgtagtg tcttgcctaa
    19501 aaaattgtct tataaggaag ctgttgctgc aagttctatc gccaatgtag cggttgctgc
    19561 cttgttggca ggagacatgg tgaccgctgg gcaagcaatc gagggagatc tcttccatga
    19621 gcgctatcgt caggacttgg taagagaatt tgcgatgatt aagcaagtga ccaaagaaaa
    19681 tggggcctat gcaacctacc tttctggtgc tgggccgaca gttatggttc tggcttctca
    19741 tgacaagatg ccaacaatta aggcagaatt ggaaaagcaa cctttcaaag gaaaactgca
    19801 tgacttgaga gttgataccc aaggtgtccg tgtagaagca aaataaagaa tagaagatag
    19861 gatggggaaa ctcttgacca gagggtttca tatccttttt gtgaaaagaa gtttatactc
    19921 aatgaaaatc aaagagcaaa ctaggaagct agccgcaggc tgctcaaaac agtgttttga
    19981 ggttgcagat agaactgacg aagtcagtaa catacctacg gtaaggtgac gctgacgtgg
    20041 tttgaagaga ttttcgaaga gtattagtta aaaacgtgat aaaggagaaa taaagatggc
    20101 agaaatttat ctagcaggtg gttgtttttg gggcctagag gaatattttt cacgcatttc
    20161 tggagtgcta gaaaccagtg ttggctacgc taatggtcaa gtcgaaacga ccaattacca
    20221 gttgatcaag gaaacagacc atgcagaaac ggtccaagtg atttacgatg agaaggaggt
    20281 gtcactcaga gagattttac tttattattt ccgagttatc gatcctctat ctatcaatca
    20341 acaagggaat gaccgtggtc gccaatatcg aactgggatt tattatcagg atgaagcaga
    20401 tttgccagct atctacacag tggtacagga gcaggaacgc atgctgggtc gaaagattgc
    20461 agtagaagtg gagcaattac gccactacat tctggctgaa gactaccacc aagattatct
    20521 caggaagaat ccttcaggtt actgtcatat cgatgtgacc gatgctgata agccattgat
    20581 tgatgcagca aactatgaaa agcctagtca agaggtgttg aagaccagtc tatctgaaga
    20641 gtcttatcgt gtcacacaag aagctgctac agaggctcca tttaccaatg cctatgacca
    20701 aacctttgaa gaggggattt atgtagatat tacgacaggt gagccactct tttttgccaa
    20761 ggataagttt gcttcaggtt gtggttggcc aagttttagc cgtccgattt ccaaagagtt
    20821 gattcattat tacaaggatc tgagccatgg aatggagcga attgaagttc gttctcgttc
    20881 aggcagcgct cacttgggtc atgttttcac agatggaccg cgggagttag gcggcctccg
    20941 ttactgtatc aattctgctt ctttacgctt tgtggccaag gatgagatgg aaaaagcagg
    21001 atatggctat ctattgcctt acttaaacaa ataaaacaga gagtggggct tcccactttc
    21061 ttcatttcta gaatatgaat agaagggatt tatgaaacac ctattatctt acttcaaacc
    21121 ctacatcaag gaatcaattt tagccccctt gttcaagctg ttagaagctg tttttgagct
    21181 cttggttccc atggtgattg ctgggattgt tgaccaatct ttacctcagg gagatcaagg
    21241 tcatctctgg atgcagattg gcctgctcct tatctttgca gtaattggcg ttttagtggc
    21301 cttgatagct caattttact cagcaaaggc agcagtaggt tttgctaagg aattgacaaa
    21361 cgatctttat cgtcatattc tttccttgcc caaggacagc agagaccgtc tgacaacatc
    21421 tagtttggtc actcgcttga cttcggatac ctaccagatt cagactggta tcaatcaatt
    21481 cctgcgtctc tttttgcgag cgcccattat cgtttttggt gccattttta tggcttatcg
    21541 aatctcaact gagttgactt tctggttctt agtcatggtt gccattttga ccattgtcat
    21601 tgtagggtta tctcgattgg tcaatcctct ctacagtagt ctcagaaaga aaacggacca
    21661 actggttcag gaaacgcgcc agcaattgca agggatgcgg gttattcgtg cttttggtca
    21721 agaaaaacga gagttacaga tttttcaaac ccttaaccaa gtttatgcta gattacaaga
    21781 aaagacaggt ttctggtcta gtttattaac acctctgacc tatctgattg tcaatggaac
    21841 tcttctcgtt attatctggc aaggctatat ttcaattcaa ggaggagtac tcagtcaagg
    21901 tgctctcatt gctcttatca attacctctt acagattttg gtggaattgg tcaagctagc
    21961 catgctgatc aattccctca accagtccta tatctcagcc aagagaatcg aggaggtctt
    22021 tgcggaatct cccgaaaaca tccattcaga attagaacaa aagcaagtta ccagtggtcg
    22081 ggttttacaa gtccaagaat tgacttttac ctatcctgat gcggcccagc cttctctgag
    22141 agacatttcc tttgatatga ctcaaggaca aatccttggt atcattgggg ggactggttc
    22201 tggtaaatca agcttggtgc aactcttact tggactttat ccagtagaca aggggaacat
    22261 tgacctttat caaaatggac gtagtcctct taatttggag cagtggcggt cttggattgc
    22321 ctatgtacct caaaaggtca aactctttaa gggaactatt cgttccaact tgactttagg
    22381 tttaaatcaa gaagtatctg accagaaact ctggcaggcc ttggagattg cgcaagctaa
    22441 ggattttgtc agtgaaaagg aaggactttt ggatgcccta gttgaggcag gggggcgaaa
    22501 tttctcaggt ggacaaaaac aaaggttgtc tatcgcccga gcagtcttgc gccaagctcc
    22561 gtttatcatc ctagatgatg caacctcggc actggatacc attacagagt ccaagctctt
    22621 gaaagctatt agagaaaatt ttccaaacac gagcttaatt ttgatctctc aacgaacctc
    22681 aactttacag atggcggacc agattctcct cttggaaaaa ggtgagttgc tagctgttgg
    22741 caagcacgat gacttgatga aatccagcca agtctatcgt gaaatcaatg catcccaaca
    22801 tggaaaggag gactagaatg aaacgacaaa ctgtaaacca gacgctcaaa cgtttagccg
    22861 tagatttagc aaaccatccc ttcctccttt tcctagcctt tctaggaact attgcccaag
    22921 ttggcttatc aatttaccta cctattctga ttgggcaggt cattgaccaa gtcctagtgg
    22981 ctggttcatc accagttttt tggcagattt ttctccagat gctcttggtg gtaataggaa
    23041 atactctggt acaatgggcc aatcctctcc tctataatcg tctaatcttc tcttatacca
    23101 gagatttgcg agagcgaatc atccataagc tccatcgttt accgattgct tttgtagata
    23161 ggcaaggtag tggagagatg gttagtcgtg taaccacgga catcgaacag ttggcagctg
    23221 gcttgaccat gatttttaac caatttttca ttggtgtttt gatgattctg gttagtattc
    23281 tagccatgct ccaaattcat ctcctcatga ctctcttagt cttgctgttg acgccactgt
    23341 ccatggtgat ttcacgcttt attgccaaga gatcctatca tctcttccag aagcaaacag
    23401 agacgagggg aattcagact cagttgattg aagaatcgct tagccagcag actataatcc
    23461 agtccttcaa tgctcaaaca gaatttatcc aaagattgcg tgaggctcat gacaactact
    23521 caggctattc tcagtcagcc atcttttatt cttcaacggt caatccttcg actcgctttg
    23581 taaatgcact catttatgcc cttttagctg gagtaggagc ttatcgtatc atgatgggtt
    23641 caaccttgac cgtcggtcgt ttagtgactt ttttgaacta tgttcagcaa tacaccaagc
    23701 cctttaatga tatttcttca gtgctagctg agttgcaaag tgctctggct tgcgtagagc
    23761 gtatctatgg agtcttagat agccctgaag tggctgaaac aggtaaggaa gtcttgacca
    23821 gtgaccaagt taagggagct atttccttta aacatgtctc ttttggctac catcctgaaa
    23881 aaattttgat taaggacttg tctatcgata ttccagctgg tagtaagata gccatcgttg
    23941 gtccgacagg tgctggaaaa tcaactctta tcaatctcct tatgcgtttt tatcccatta
    24001 gctcgggaga tatcttgctg gatgggcaat ccatttatga ttatacacga gcatcattga
    24061 gacagcagtt tggtatggtg cttcaagaaa cctggctcac acaagggacc attcatgata
    24121 atattgcctt tggcaatcct gaagccagtc gagagcaagt aattgctgct gccaaagcag
    24181 ctaatgcaga ctttttcatc caacagtttc cacagggata cgataccaag ttggaaaatg
    24241 ctggagaatc tctctctgtc ggccaagctc agctcttgac catagcccga gtctttctgg
    24301 ctattccaaa gattcttatc ttagacgagg caacttcttc cattgataca cggacagaag
    24361 tgctggtaca ggatgccttt gcaaaactca tgaagggccg cacaagcttc atcattgctc
    24421 accgtttgtc aaccattcag gatgcggatt taattcttgt cttagtagat ggtgatattg
    24481 ttgaatatgg taaccatcaa gaactcatgg atagaaaggg taagtattac caaatgcaaa
    24541 aagctgcggc ttttagttct gaataagcca ttctcttttg aaagtttatg gacgaaaaaa
    24601 gttgccttcg agtgactttt ttgttacaat agctagaaaa attgttcact gtaatactca
    24661 atgaaaatca aagagcaaac taggaagcta gccgtaggtt gctcaaaaca gtgttttgag
    24721 gttgcagata gaactgacga agtcagtaac cacacctacg gcaaagtgaa tttgaagtgg
    24781 tttgaagaga gtacaacttg tcttttagaa aaggagccta taatgaaagt ctttcagcat
    24841 gtaaatatcg tgacttgtga tcaagatttc catgtttatc ttgatggaat cttagcagtc
    24901 aaggattctc aaatcgtcta tgtcggtcaa gataagccag cgtttttaga acaagctgag
    24961 cagattatag actatcaggg agcttggatt atgcctggtt tggtcaattg tcacacccat
    25021 tctgcaatga caggtctgag agggatccga gatgacagca atctccatga atggctcaat
    25081 gactatatct ggccagcaga atctgagttt actcccgaca tgactaccaa tgcggtcaaa
    25141 gaagccctaa cagagatgct ccagtcagga acaacaacct ttaacgatat gtataatccc
    25201 aatggtgtgg atatccagca aatttatcag gtggtgaaaa cttccaagat gcgttgttat
    25261 ttttctccga ctctcttttc ttcagagaca gaaacaactg ctgagactat aagcagaact
    25321 cgatccatca tagacgaaat cttaaaatat aaaaatccaa atttcaaggt tatggtagca
    25381 cctcattctc cgtatagctg cagtagagac ttgctggaag cgagtttgga aatggcaaaa
    25441 gagctaaata ttccgctcca tgtccatgtg gcggagacca aggaggagtc aggaattatc
    25501 ctcaaacggt acggcaaacg cccccttgct tttcttgaag aactgggtta tttagatcat
    25561 ccgtccgtat ttgctcacgg ggtcgaatta aacgagagag aaattgaact cttggcatct
    25621 tctcaagtgg ctatcgccca caatcctatc agtaacctca aactggcatc aggaattgct
    25681 ccaattatcc agctccaaaa agcgggagta gtagtcggaa ttgcgactga ctcggttgct
    25741 tccaataaca atctagatat gtttgaggaa ggaaggactg cagctcttct tcagaagatg
    25801 aaaagtgggg atgccagcca gtttccaatc gaaacagctc tcaaggtact gacaatcgaa
    25861 ggggctaagg tccttggaat ggaaaatcag ataggaagtc tggaagtcgg caagcaagca
    25921 gattttctgg tcattcaacc acaagggaaa attcatctcc aacctcagga aaatatgctg
    25981 tctcacctgg tttatgcagt taaatctagt gatgtagatg atgtttatat cgccggagaa
    26041 caggttgtta agcaaggtca agtcctgaca gtagaacttt aaaagaaaaa tcacgaaaaa
    26101 ttttaaaaaa agtttgcaaa aatcttgcat tctttttttg actatgctat acttatatac
    26161 ggtttaaaaa aactgcctaa gacagtaggg gagctcgact cataaatatc ctaccgagga
    26221 caaaacgtat catgtaaaaa gaagcgtatt gtactttcgt gtctaggttt gggcgcgttt
    26281 ttctttttga aaaattcccc aagcaaaata attacggagg tgaacacact aatgagtgaa
    26341 gcaattattg ctaaaaaagc ggaactagtt gacgtagtag ctgaaaaaat gaaagctgct
    26401 gcatctatcg tcgttgtaga cgctcgtggt ttgacagttg agcaagatac agttcttcgt
    26461 cgtgagcttc gtggaagcga agttgagtat aaagttatta aaaactcaat cttgcgtcgt
    26521 gcagctgaaa aagctggtct tgaagatctt gcatctgtat ttgttggacc atctgcagta
    26581 gcattttcta atgaagatgt tatcgcacca gcgaaaatct tgaacgactt ttctaaaaac
    26641 gctgaagcac ttgaaattaa aggtggtgca atcgaaggcg ctgtcgcatc taaagaagag
    26701 attcttgcac ttgcaactct tccaaaccgc gaaggacttc tttctatgct cctttctgta
    26761 cttcaagcgc cagtgcgcaa cgttgctctt gcagtcaaag cggttgcaga aagcaaagaa
    26821 gacgcggctt aatcttaagc tacacagcgt agcctagcta cgaaaaaaac tattataaaa
    26881 tttaaaactt atttggagga aataacaatg gcattgaaca ttgaaaacat tattgctgaa
    26941 attaaagaag cttcaatcct tgaattgaac gaccttgtaa aagctatcga agaagaattt
    27001 ggtgtaactg cagctgctcc tgtagctgtt gctgcagctg atgcagctga tgctggtgct
    27061 gctaaagatt cattcgacgt tgaattgaca tctgcaggcg acaaaaaagt tggcgttatc
    27121 aaagttgtac gtgaaatcac tggtcttggt cttaaagaag ctaaagaact tgttgacggt
    27181 gcaccagcac ttgttaaaga aggcgttgca actgcagaag ctgaagaaat caaagctaaa
    27241 ttggaagaag ctggagcttc agttactctt aaataataga gtatcgcaat ttgattgcgg
    27301 aatccctatt taccagtctt tgagagcgat aagcgattga tagaaactga taaattgatt
    27361 tggtttcctg ccaaaaattc tttggcagga tttttgtttt aataaacttt cgggggtaga
    27421 cactagattg tctacccctt tttttgtgtt tcggaggtaa ttatgcaaaa ggaaggacag
    27481 tcatcacgtc aattcatgaa gagtcatctc atgactatca tgggagttga tattgtaaaa
    27541 gcaactcagt tagttgatga aatggaacaa gtaggtttaa tccgatttga tgaatttgga
    27601 aatgttggaa ttttagtctt ggagggacaa taatgaagcg tattaccgct aatcaatatc
    27661 agacatcaga gcgttattat aagctcccca aacttttatt tgagagtgaa cggtataaga
    27721 atatgaaacc ggaggttaag gtggtttact cagttttaaa agatcggttg gagttgtctt
    27781 tgagcaaagg ttggattgat gaggatggga ctatttattt gatttattcc aattcaaatt
    27841 tgatggcact tttaggctgt tcaaagtcaa aattactctc catgtgagtt tgaagtgaca
    27901 tttttagatg attaccataa aaaacataac tacccactat tttacgaatc ctatcttcaa
    27961 aacgttatgg aattccttga aagtcaagac ataaagaatg gggttgatgc ctttgtagat
    28021 gatcatcaaa atctcgtttt tgttttatat ggacaaggct atcgagccga gggaaaagag
    28081 ggaatactta caacccaagt aactgtaaaa gcttatgatg aagacaagaa accgattaac
    28141 ttcgcaaatt tattagattc cttaatcgtg tcagaatatc aaatggaacc gaatctttgg
    28201 gaggtctcct atgattgatc tctatctaag taaaaatagc cgaagaaatc aacttctttt
    28261 agacttcttc caaaactatg gcatcgaggt atcttgtcat tcagtttctg aaatgacaaa
    28321 ggacaaatta attgagatga tgagctattc ttcagattgt tttgagtttt tatctccaaa
    28381 tttattacgt tttaagaatc gagataacct aaaattaaca gatttcatcg aaatgatatt
    28441 aaaaaatcct gaactaacca tcagacttcc tcttgcggtt tcaaataagc gagtttatcc
    28501 aagtctgaat ttggaagagg ctagagctct attgccaaga gatacgaaac aattgattta
    28561 catggcgcaa acacattatt tatctaacta aaggagaagc aatatggctg aacgattttg
    28621 ggagaacttg tccattattt tggctgaaag aaatatcagc tggattgagc taaccagaaa
    28681 aatgtttgcg ggagagtttc actatccaag tgaattgaat cgtttgtatc aaaagattcg
    28741 tcactacaag atggaacaac gaatgcctca aagtccatgg gtagaaagga ttgtgcaggt
    28801 attagattta gattatgaag atttatttcg gaggtaacta tgaaaagaat aattccagtt
    28861 tatatattcc aacaagtaaa tgtcctattg gtatctctat acttactgaa atttctttgt
    28921 atcggtgagt taactatact acagattcta tattgtgcgt cactcatttc ttttttatgg
    28981 atgtatgccc aacgtaaaca agcgcataag gtcaatatga aattaaggat aaaatggatt
    29041 ggtattggat tagccagtct actgattata agtctatgtt ttagtctaat ccatgcgcaa
    29101 gaaagcacga atcaagcaaa cttaattggg cttcaatatc aagttccttg gttttcattt
    29161 ttattgttct taatcaatgc gagtatggtt gaagaatttc tgtatagaga aattttatgg
    29221 aacttggtta gaaaattaga tattcgagtt gctttgacaa gtgttttatt tgccttagca
    29281 catcatccag gaaccattat agcttggtgt ttgtatgtat cgctgaggat gtttttaggg
    29341 cttgtacgct acaaattgga cttatggggc agtatggggc tacatttggt gtggaaccta
    29401 ctagtttata gtttgctgct tttttaaaat aggtaacctg atttttaggt aacttctaaa
    29461 actaacttcc agttttggga gagagatgga agttactttg aaaagttacg gaaacattat
    29521 tttctatgtg acaacattgt atcattgctt gcattttact gctggagaga gtataatgaa
    29581 tgtggataaa gcacttaatc ccaaaaattt aaataggagg agttagctat ggcagaagaa
    29641 gtattaaatc tttaattagt atctgttcaa gttgacgaaa cagatgaagt tgatggaatg
    29701 cgatttagta cattcagtac aaaccgttgc ggtaactgga gtgcttttag ttgggaaaat
    29761 tgttaattca tttaaattgt aaactttaga gattctctgg agttatggat gtcgttccaa
    29821 tatgtgcacg ttggaatgtt agtgcttata tggggggaat ccaaaggatt ctcccttttt
    29881 attttaataa gtctgtctag ctatagatat attaagtttg atagaatttc ttggagattt
    29941 tgttatggat tataatttca atttggagca tcctttcttt ttcactaata atgattatag
    30001 tacagatact agcataaagt actaagtaag tctgcctttt aattggcacg aagtaatgaa
    30061 taatgatgag tgggtttatc aataccctat tggaaaattt gttgaaagac aagggtggaa
    30121 aatacacatt agttcagagt ataatagttc acacgaattg ttacaagatg tagcaaaaat
    30181 ttgccatgaa atgcgaattc catttaaaca tcttagtaca gaagacaaat ttatcatgcg
    30241 aaacgggaaa ttggttagtc gaggattttc tggtaaattt attacatgtt atccaaatca
    30301 aaatgaatta gagtcggttt tgcaaaagtt agaaagtgcc ttaaagcaat ataatgggcc
    30361 atatatttta agctataaac gttgggatga agtaccaata tatctgaggt atggtgtgtt
    30421 taggccgtca agggatgatg aaaagaaggt tgctattgat gaattaattg ttggcgatga
    30481 agttgttaaa gatgaacgtt taccagtatt taaaattcca aaaggtatag tgctccctga
    30541 ctttttgaat aaatggcttg acaagaaaga taaaaaacag ggagactttc cctttattat
    30601 agataatgca attagatttt caaacagtgg tgggatatat aatgctagac taaaagaaga
    30661 tgggaaaaaa ataattttaa aagaggctag accgtataca ggtctaggat ttgatggtac
    30721 ttattcttca gagagattag cttcggagtg caaagcacta aaaattctaa atgaatggag
    30781 tgagacgcct aaaatatact ggtgcggtaa gatctgggag cacaccttct taggaattga
    30841 acatatgaag ggcgttccac taaatagatg ggttaccaat aattttcctt tatatgaagt
    30901 tgttgataag accaaagatt atctattgag agtttcaaag atagtagaaa aactcatcga
    30961 tttaacgaat aagtttcatt ctgaaaatgt atatcatcaa aatttacatt taggaaatat
    31021 cttggttaag gatgaagatg agattagtat aatagactgg gaacaggctg tcttttcgaa
    31081 tgatgaaaaa gttgtacata aagttgcagc ccctggtttt agagcatggc gagaaacact
    31141 tcccagtgag atagattggt atggtattag acaaatagct cattatttat acatgccact
    31201 agtaactaca tcggatttga catacaacta tgtaagtcaa accaggatag aaggaaaaaa
    31261 actatttgag tctttgggtt atacaagaga acatatagat tatgtagaaa gtttattgtc
    31321 atatctagat tctaaatgtc cacagattga gaatatttcc aggaaaaaag ttttaaaacc
    31381 catgcatgag attagaacta tcgagagtga gcaagatata caagatttta ttataaaagc
    31441 ctttctttta gtgaactgat aggtattttc ttagcaaacg ataaagtttt agggccgata
    31501 caatcaattg cctattcatt aaataagata aatacaacca aagatttaag gaaaccgttt
    31561 ttaaaatact taagtggaga gaagaatttt atagacgctg aacatgataa taacggactg
    31621 tatacttcat caatagatga gatacacatg aaagatgttg tatattctat tacaccagaa
    31681 aataaattaa gtattgactt ctcatttaag tcaccattta gggtattatt aacaggaact
    31741 tctggtagtg ggaaaacaac gattttaaat ttaattaatg gttctttaaa gccacaaaaa
    31801 ggttatgtaa atttgttatc acatgggaaa aagagttcag attcaatacc aacagttgat
    31861 cagacaccat atatttttga cactactatt cgtgagaacg taactttatt tcaaaatgaa
    31921 tatttttcag atgatcagat aattgaggtg ttaaaaaagg taaatctata tgaagaatta
    31981 gaaaagatag atatactaaa ttatcaatgt ggtgaaaatg gtagtaattt gtctggaggt
    32041 caaaaacaaa aaatagcttt agctagagct ctgattagaa ataataaagt gtacttattt
    32101 gacgaaatat cagctaattt agataatgat aattcaaatt ccatacatga tattctgttc
    32161 aatttaggta tttcatttat tgaagtttca catcattatg acttaaatga caagagatac
    32221 actgatatat ataaattgga aaatggaaca cttttcaaaa ttaagtgatt ggaggcaaga
    32281 atatggataa agattatata ttaaaagtga aagggctgta tcatcaattt ttactaggaa
    32341 ataataaaac gttgcaagtg ctgaaaaatg tttctctttc tgcttcgaga ggagaattta
    32401 taagtattct aggaattagt ggttctggaa agtcaacttt attaaaatgt atttctagtt
    32461 tgcttgaacc aacaagtggg gaagtaattt taaatggaat caacccctat aaaatcagaa
    32521 atgcaaaatt gtcgagtata agacgtaacg aagtatcttt tatatttcaa gcatacaatt
    32581 taataccttc cctgccggta atagaaaata tagcacttcc tttgcgatta tcacaaaaaa
    32641 aattaactat taaaaatgta gaaaacttac tcaaaagaat gaagtttaat gctggcttaa
    32701 acgattttgt tggaactctg tctggtggag aacaacagaa ggttgctata gctagagcgg
    32761 ttattgctga tagtgatata atatttgctg atgagccaac tggggcttta gacagcgttt
    32821 ctcgtgaagt aatttttgaa ttattgagag agttagtagg ggcgggtaag tgtgtaatta
    32881 tggtaacgca cgatatagaa ttggcctcga aaactgatcg tgcattaata ttgaaagatg
    32941 gaaaaatttt caaagaactt catagaccta gcggggaaga gttgtataaa atcttagagg
    33001 tacaatcaac tacggaggaa tagagatgct cagattagtt tactatcaat ttttacataa
    33061 taagaaacaa tggttaggcg tttcaccagt aatttttgtt tcgagtttag ttatgggatt
    33121 ggctgttaat ggggtaatta atgttgagaa taattcgcaa gtatttgtcg gacttccaga
    33181 tcctaaacca atttttatgt ttccgatagt attcggagga gtaacattat tctttgtgct
    33241 ctctaacata ataaatatgt tggttgaaat ttttagagat gattacgaac ttctggaggt
    33301 gttgggagca agtctccttc aactatcatt tcttgtgggg ggacagattt ttatcatttc
    33361 ctcgattatt agttttattg catatctatg ctctatattt gttacgtcaa attattatta
    33421 ttttttacaa tactttttcg gggaaaatat attacctgat atccaatttc aaacgagtgc
    33481 tgttggatgt atcattacag tagtgttgat ttcattttta gcctttctaa gtggttgctt
    33541 ttatacgttc aagaagatta gaaataggaa gagttctaaa ataaggcatg ttctttcaat
    33601 agtaaaaaga atactgctat tggctggttt tagtgttatc tggttgcttt ctttgcaaca
    33661 aattttccaa gatagcacaa ttttagcgaa ggcccagata atattcaata ttgtaatctt
    33721 agatattgtg attatttatc aattatctcc ttttatacag tcatgtttta tcaaactact
    33781 ttctataatt atatttagaa ataattttat gttcattgtt tcgaagtgga atttactata
    33841 tcgtaagcct tatataaaat ctatcagtgc tgcaattaca ggagctatat tactaatttc
    33901 tagttttcaa atgatttctc agaatatact atctcaattt caagatgaca gtgatttaga
    33961 gctaaaagtg gcatttattg tctatgtagg agcaccaatt ttgatagtac ttgcaaatat
    34021 tatttcaatt gcatttttgt catcgcatca agaaaggatt gagattcagc aatttgaaat
    34081 tttaggaact tctaattacc agatggttaa gattaaagtt ggtgaggcta tatttttgac
    34141 atttgttact tcattaattg catttttact taacattaaa attatggctt taatatacta
    34201 ctctttagag gatattttaa tagatgatat gaatcttttg gggttaatac ttcc
//
LOCUS       DAVKQL010000020        33917 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVKQL010000020 DAVKQL010000000
VERSION     DAVKQL010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33917
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.020"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            60..656
                     /locus_tag="VW561_000857"
                     /pseudo
     CDS             60..656
                     /locus_tag="VW561_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            813..1187
                     /locus_tag="VW561_000858"
     CDS             813..1187
                     /locus_tag="VW561_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9943141.1"
                     /translation="MAVGLSYEDPAVKLLNDGANIKVVYPKEGTVFLPASAAIVKKAK
                     NMENAKKFIDFIISQEVQDTLGTTTTNRPVRKNAKTSENMKPIDKIKTLTEDYDYDYV
                     IKNKSDIVKKYNEVFTDIQSKQ"
     gene            1200..2231
                     /locus_tag="VW561_000859"
     CDS             1200..2231
                     /locus_tag="VW561_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9943142.1"
                     /translation="MSEIKIINAKKIYHDVPVIENLNITIPKGSLFTLLGPSGCGKTI
                     LLRMIAGFNSIEGGEFYFDDTNINNMEPSKRNIGMVFQNYAIFPHLTVRDNVTFGLMQ
                     KKVPKEELIQQTNKYLELMQIAQYADRKPDKLSGGQQQRVALARALAVNPSVLLMDEP
                     LSNLESKLRLDIHQAIREIQHEVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRP
                     KELYHKPANEFVATFIGRTNIIPANLEKRSDGAYIVFSDGYALRIPALDQVEEQAIHV
                     SIRPEEFIKDESGDIEGTISDSVYLGLNTEYFIETGFASKIQVSEESTFEEDLQKDNR
                     IRLRINTQK"
     gene            2281..3933
                     /locus_tag="VW561_000860"
                     /pseudo
     CDS             2281..3933
                     /locus_tag="VW561_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(4036..4728)
                     /locus_tag="VW561_000861"
     CDS             complement(4036..4728)
                     /locus_tag="VW561_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9943143.1"
                     /translation="MVKVRLYLIRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(4972..6309)
                     /locus_tag="VW561_000862"
     CDS             complement(4972..6309)
                     /locus_tag="VW561_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEU9943144.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGKEGDMIEFGGLLGTAPVMRVNGASSVDFISRGGQIPAPIHSFKN"
     gene            complement(6319..6585)
                     /locus_tag="VW561_000863"
     CDS             complement(6319..6585)
                     /locus_tag="VW561_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEU9943145.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAIVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            complement(6850..7236)
                     /gene="rplQ"
                     /locus_tag="VW561_000864"
     CDS             complement(6850..7236)
                     /gene="rplQ"
                     /locus_tag="VW561_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU9943146.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIVELV"
     gene            complement(7248..8183)
                     /locus_tag="VW561_000865"
     CDS             complement(7248..8183)
                     /locus_tag="VW561_000865"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU9943147.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            complement(8226..8609)
                     /gene="rpsK"
                     /locus_tag="VW561_000866"
     CDS             complement(8226..8609)
                     /gene="rpsK"
                     /locus_tag="VW561_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEU9943148.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(8627..8992)
                     /gene="rpsM"
                     /locus_tag="VW561_000867"
     CDS             complement(8627..8992)
                     /gene="rpsM"
                     /locus_tag="VW561_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEU9943149.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(9010..9126)
                     /gene="rpmJ"
                     /locus_tag="VW561_000868"
     CDS             complement(9010..9126)
                     /gene="rpmJ"
                     /locus_tag="VW561_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEU9943150.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            complement(9151..9369)
                     /gene="infA"
                     /locus_tag="VW561_000869"
     CDS             complement(9151..9369)
                     /gene="infA"
                     /locus_tag="VW561_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU9943151.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(9486..10124)
                     /locus_tag="VW561_000870"
     CDS             complement(9486..10124)
                     /locus_tag="VW561_000870"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEU9943152.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLN
                     ELGIELEGVINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            complement(10275..11585)
                     /gene="secY"
                     /locus_tag="VW561_000871"
     CDS             complement(10275..11585)
                     /gene="secY"
                     /locus_tag="VW561_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEU9943153.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            complement(11598..12038)
                     /gene="rplO"
                     /locus_tag="VW561_000872"
     CDS             complement(11598..12038)
                     /gene="rplO"
                     /locus_tag="VW561_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEU9943154.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            complement(12183..12407)
                     /gene="rpmD"
                     /locus_tag="VW561_000873"
     CDS             complement(12183..12407)
                     /gene="rpmD"
                     /locus_tag="VW561_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEU9943155.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGCIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            complement(12379..12873)
                     /gene="rpsE"
                     /locus_tag="VW561_000874"
     CDS             complement(12379..12873)
                     /gene="rpsE"
                     /locus_tag="VW561_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEU9943156.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            complement(12891..13247)
                     /gene="rplR"
                     /locus_tag="VW561_000875"
     CDS             complement(12891..13247)
                     /gene="rplR"
                     /locus_tag="VW561_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEU9943157.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            complement(13331..13867)
                     /gene="rplF"
                     /locus_tag="VW561_000876"
     CDS             complement(13331..13867)
                     /gene="rplF"
                     /locus_tag="VW561_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU9943158.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEVTLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            complement(14059..14457)
                     /gene="rpsH"
                     /locus_tag="VW561_000877"
     CDS             complement(14059..14457)
                     /gene="rpsH"
                     /locus_tag="VW561_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEU9943159.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            complement(14742..15011)
                     /gene="rpsN"
                     /locus_tag="VW561_000878"
     CDS             complement(14742..15011)
                     /gene="rpsN"
                     /locus_tag="VW561_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEU9943160.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            complement(15029..15571)
                     /gene="rplE"
                     /locus_tag="VW561_000879"
     CDS             complement(15029..15571)
                     /gene="rplE"
                     /locus_tag="VW561_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEU9943161.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            complement(15595..15900)
                     /gene="rplX"
                     /locus_tag="VW561_000880"
     CDS             complement(15595..15900)
                     /gene="rplX"
                     /locus_tag="VW561_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEU9943162.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            complement(15978..16346)
                     /gene="rplN"
                     /locus_tag="VW561_000881"
     CDS             complement(15978..16346)
                     /gene="rplN"
                     /locus_tag="VW561_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEU9943163.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            complement(16372..16632)
                     /gene="rpsQ"
                     /locus_tag="VW561_000882"
     CDS             complement(16372..16632)
                     /gene="rpsQ"
                     /locus_tag="VW561_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEU9943164.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            complement(16657..16863)
                     /locus_tag="VW561_000883"
     CDS             complement(16657..16863)
                     /locus_tag="VW561_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEU9943165.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            complement(16873..17286)
                     /gene="rplP"
                     /locus_tag="VW561_000884"
     CDS             complement(16873..17286)
                     /gene="rplP"
                     /locus_tag="VW561_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEU9943166.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            complement(17290..17943)
                     /gene="rpsC"
                     /locus_tag="VW561_000885"
     CDS             complement(17290..17943)
                     /gene="rpsC"
                     /locus_tag="VW561_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEU9943167.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(17956..18300)
                     /gene="rplV"
                     /locus_tag="VW561_000886"
     CDS             complement(17956..18300)
                     /gene="rplV"
                     /locus_tag="VW561_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEU9943168.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            complement(18312..18593)
                     /gene="rpsS"
                     /locus_tag="VW561_000887"
     CDS             complement(18312..18593)
                     /gene="rpsS"
                     /locus_tag="VW561_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEU9943169.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            complement(18697..19530)
                     /gene="rplB"
                     /locus_tag="VW561_000888"
     CDS             complement(18697..19530)
                     /gene="rplB"
                     /locus_tag="VW561_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU9943170.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            complement(19548..19844)
                     /locus_tag="VW561_000889"
     CDS             complement(19548..19844)
                     /locus_tag="VW561_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEU9943171.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(19844..20467)
                     /gene="rplD"
                     /locus_tag="VW561_000890"
     CDS             complement(19844..20467)
                     /gene="rplD"
                     /locus_tag="VW561_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEU9943172.1"
                     /translation="MANVTLFDQTGKEAGQVVLNDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQVAISKIEEVLA"
     gene            complement(20492..21118)
                     /gene="rplC"
                     /locus_tag="VW561_000891"
     CDS             complement(20492..21118)
                     /gene="rplC"
                     /locus_tag="VW561_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEU9943173.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            complement(21335..21643)
                     /gene="rpsJ"
                     /locus_tag="VW561_000892"
     CDS             complement(21335..21643)
                     /gene="rpsJ"
                     /locus_tag="VW561_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEU9943174.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(21909..22526)
                     /locus_tag="VW561_000893"
     CDS             complement(21909..22526)
                     /locus_tag="VW561_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEU9943175.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRHLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKAGEVLSGTKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            complement(22523..23113)
                     /gene="nrdG"
                     /locus_tag="VW561_000894"
     CDS             complement(22523..23113)
                     /gene="nrdG"
                     /locus_tag="VW561_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEU9943176.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            complement(23106..23612)
                     /locus_tag="VW561_000895"
     CDS             complement(23106..23612)
                     /locus_tag="VW561_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9943177.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKSQSAHDGGFWDTENFVYEEWL
                     ETNIQKEMGINLPENRVPSIQFVLFGESGCALRFLNLRLRLNERLLNYAGHIGYSIRP
                     SEKGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDAHNGVER
                     YWIEVANE"
     gene            complement(23656..23796)
                     /locus_tag="VW561_000896"
     CDS             complement(23656..23796)
                     /locus_tag="VW561_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943178.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            complement(23808..26015)
                     /gene="nrdD"
                     /locus_tag="VW561_000897"
     CDS             complement(23808..26015)
                     /gene="nrdD"
                     /locus_tag="VW561_000897"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEU9943179.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKWEDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHHLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            complement(26135..27673)
                     /locus_tag="VW561_000898"
     CDS             complement(26135..27673)
                     /locus_tag="VW561_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU9943180.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLFLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            complement(27710..27853)
                     /locus_tag="VW561_000899"
     CDS             complement(27710..27853)
                     /locus_tag="VW561_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943181.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            complement(27931..29463)
                     /gene="cls"
                     /locus_tag="VW561_000900"
     CDS             complement(27931..29463)
                     /gene="cls"
                     /locus_tag="VW561_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEU9943182.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            complement(29609..30151)
                     /locus_tag="VW561_000901"
     CDS             complement(29609..30151)
                     /locus_tag="VW561_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEU9943183.1"
                     /translation="MTPFFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDIDG
                     TYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVNG
                     NQIVVDDMDRDPSDQIVLTK"
     gene            complement(30148..31398)
                     /locus_tag="VW561_000902"
     CDS             complement(30148..31398)
                     /locus_tag="VW561_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU9943184.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRIQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKE
                     MEKTLLQTPDQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLICQKKAESLAVDLYQA
                     GQDFSVLNGDFSSSLLNLSQLNIGLEGAYQQENAALALQTFLLFMGERKEAIDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTCLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            complement(31705..32010)
                     /locus_tag="VW561_000903"
     CDS             complement(31705..32010)
                     /locus_tag="VW561_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEU9943185.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            complement(32026..32445)
                     /gene="ruvX"
                     /locus_tag="VW561_000904"
     CDS             complement(32026..32445)
                     /gene="ruvX"
                     /locus_tag="VW561_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEU9943186.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            complement(32449..32715)
                     /locus_tag="VW561_000905"
     CDS             complement(32449..32715)
                     /locus_tag="VW561_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEU9943187.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            complement(32802..33371)
                     /locus_tag="VW561_000906"
     CDS             complement(32802..33371)
                     /locus_tag="VW561_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU9943188.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQQGVE
                     ETQKALLEAEEKDKSIIEARKLVGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            complement(33437..33835)
                     /gene="spx"
                     /locus_tag="VW561_000907"
     CDS             complement(33437..33835)
                     /gene="spx"
                     /locus_tag="VW561_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEU9943189.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
BASE COUNT         9661 a         7506 c         6201 g        10549 t
ORIGIN      
        1 tttaaaaacg cttacattgt tttaaaataa acttaaataa cacaacggag gtatccatca
       61 tgaaaaagaa atggatgtat tatgctgctt gttcttctaa tgaatctgcc gatgacagtt
      121 catctgataa aggagacggt ggttcgctag tcgtttattg accaaactca gaggacttaa
      181 ttggagcaac tattcctgcc tttgaagaaa aatatggtat caaagtagaa ctgattcaag
      241 ctggtactgg agaacttttc aaaaaactag agtcagaaaa agaagttcct gtagctgatg
      301 ttatctttgg tggttcttat acacaatatg ctacccacgg agaactcttt gaaaactata
      361 cttcaaaaga aaatgataat gttatcaaag aatatcaaaa cacaactggc tactctactc
      421 cttatacact agatggtagt gttttaatcg tcaaccctga tttaactaaa ggcatgaaca
      481 tcgaaggata tagcgacctt ctcaaacctg aactaaaagg aaaaatcgca actgctgacc
      541 cagcaaactc ttctagcgcc tttgctcaat taacaaatat gctacaagct caaggtggtt
      601 acaaagatga taaggcttgg tcttatgtaa aagatctttt cacacttatt gatggtatag
      661 taaaatgaaa taagaacagg acaaattaat caggacagtc aaatcgattt ctaacaatgt
      721 tttagaagta gaggtgtact attctagttt caatacacta taaaatcggt tcaagttcat
      781 ctggtgtcta taaagcagtc gttgatggag aaatggctgt tggtctctct tatgaggatc
      841 cagcagttaa actcttaaat gacggagcta acattaaagt agtctatcca aaagaaggaa
      901 ccgtcttcct acctgctagt gctgctatcg ttaaaaaagc taaaaatatg gaaaatgcca
      961 agaaatttat cgattttatt atctctcaag aagtacaaga tacacttggt acaaccacta
     1021 ctaaccgtcc tgttcgtaaa aatgctaaaa caagcgaaaa catgaaacca attgacaaaa
     1081 tcaaaacact cactgaagat tatgattatg attatgtcat caagaataaa tcagatatcg
     1141 ttaagaaata caacgaagtc tttacagata tccaatctaa acagtaaaag aggttcacta
     1201 tgagtgagat caaaattatt aacgccaaaa aaatctacca cgatgtccct gttattgaga
     1261 atttgaacat tacaattcca aaaggaagtc tctttaccct tcttggacct tcaggatgtg
     1321 ggaaaacgat ccttcttcgt atgattgcag gtttcaacag tatcgaaggt ggagaatttt
     1381 acttcgatga tacaaacatc aataatatgg aacccagcaa acgcaatatc gggatggttt
     1441 tccaaaacta cgctattttc ccacatttga ctgtccgaga caacgttact tttggtctta
     1501 tgcaaaagaa ggttccaaaa gaagaattga ttcaacagac caacaagtat cttgaactta
     1561 tgcaaattgc tcaatatgcg gatcgaaagc ccgataaact cagtggtgga caacaacaac
     1621 gtgtcgcctt ggcacgcgcc ttagcggtta atccaagtgt tctcctcatg gacgagccac
     1681 ttagtaatct ggagtccaaa cttcgcttgg atatacatca agccatccga gaaatccaac
     1741 acgaagtggg aattacaact gtttatgtaa cccacgacca agaagaagcc atggctattt
     1801 cagaccaaat tgctgttatg aaagatgggg tgatccaaca aatcggccga ccaaaagaac
     1861 tctatcataa accagctaat gagtttgtag caacctttat cggacgcaca aatattatcc
     1921 ctgccaatct tgaaaaacgg agcgacggcg cttatatcgt cttttcagat ggctatgccc
     1981 ttcgaatacc agctcttgat caggttgagg agcaagctat tcatgtaagc attcgtcccg
     2041 aagagtttat caaagatgaa tctggagata tcgaaggaac tattagcgat agcgtctatc
     2101 ttggactaaa tacggagtat ttcattgaga caggttttgc ctcaaaaatt caagttagtg
     2161 aagaatcaac ttttgaagaa gatctacaaa aagacaatcg tattcgtcta cgaatcaata
     2221 cgcaaaaatg aaacatcttt tctgcagatg gttcccaaaa cctgataaaa ggagtcaacc
     2281 atggaatgta aaaaactaaa tatttggaca gcctcctctt tcttcatctt tcttacctat
     2341 cttgtctttc tcgtttatcc tatcgttacc gtgctcaagt aagcacttat acatgaagga
     2401 taattctcac tagctaattt tgtcactttc tttagtaaag cctactactc tgagacacta
     2461 gtcaacagtt tcagggtttc cattaccgct actgtcactt ccttagttgt aggaacccta
     2521 ttagcttatc tcttctctat gtatgacttc aaggggaaga aatttctaca aatattgatt
     2581 atcattgctt ccatgtcagc tcctttcgta ggagcctact cctggattct cttgctggga
     2641 cgaaatgagg tcatcactaa atttttgaca aatgcccttc atcttccagc tatcgatatt
     2701 tatggattca aaggaattat acttgtcttt acactgcaac tattcccact ggtatttcta
     2761 tatgttgctg gaacaatgaa cagtattgac aattctctat ttgaagcagc tgaaagcatg
     2821 gggtccttcg gatttaaacc tatcgtaacg gttgttttac ctctcctagt tccaacctta
     2881 ctagcagctc cttgcttgta tttatgagag cattctcaga ctttggaacg cctatgttga
     2941 ttggcgaagg atatcggact ttccctgtcc tgatttatac ccaatttatt agcgaggttg
     3001 gaggaaattc tgcttttgca attatggcga ttatcattgc cttggcaatt ttccttatcc
     3061 aaaaacacat tgcaaaccgc tacagtttca gcatgaatcc gctccatcca attgagccta
     3121 aaaaaactac aaaaggaaaa atggctgcca tttatgcaac agtctacgga attatcttta
     3181 tctctgtttt acctcaaatc tacttaattt atacctcttt cctaaaaaca tcaggtatgg
     3241 tatttgttaa aggttattct ccaaatagtt acaagctagc tttcaatcgt atgggatctg
     3301 ctattttcaa taccattcgt atccctttga ttgccttagt tctagttgtt ctatttgcga
     3361 catttatctc ctacctagcc gttagaaaac ggaatttgtt tacaaactta attgacagcc
     3421 tcagtatggt accttatatt gtaccaggaa ccgttctagg gattaccttc atttcttcct
     3481 tcaatactgg tctatttgga agtggatttc ttatgattac agggactgct ttcatcttga
     3541 ttatgtctct atctgtcaga agattacctt atactattcg ctcatctgtt gctagcttac
     3601 aacaaatagc accaagtatt gaagaagctg ctgaaagctt aggaagtagt cgtctcaata
     3661 cctttgctaa gattacaact ccaatgatgc tatctggtat catttctgga gccatcttat
     3721 cttgggtcac aatgatttca aaactctcta cttctatcct ctatcctcct ctacaatgtc
     3781 aaaacaagaa caatgactgt agctatttat acagaggttc tcagaggaaa ttacggtgta
     3841 gccgcagcct tgtcaactat cctgactgtt ctaacagtag gttccttgct cttgtttatg
     3901 aaaatctcta aaagcaatag cattacactt tagttttcca catcaaaaac agccgaaagg
     3961 agtaccctcg gctgtttttt cttatcttga tattcttatc aaatccctag ctcatggcaa
     4021 gcaagtctag actgattaaa tagagccttc ttccatcttc tcacgtccta gctctcggta
     4081 actacggtca ccgacaactt caaccctaaa ctgaccgtcc tcatattcaa gaatcgtcac
     4141 gctaccatta tctagaccat gcggatgcat gccattaatc agataaacaa tggttccaat
     4201 agtcattcca tggctgacaa caagggcgtt acctccacct tgatcttcca tttcttttgc
     4261 aatcatttca aagccttcct tgattcggcc actgagtttt tcccagcctt cagcccaacc
     4321 agctgtatcg acctctacca agccctcagc cagttcagca taagacaatt ggtggacgtg
     4381 gtccacatta aagatacgag gaataatgcc catgaaaaga tcgccatcat aggctccatc
     4441 aaaactaccg aaacaccatt ctctgatacg cttgtccatg cgataaggga ttttcccctg
     4501 caagccaagt tcttcaagta taattcccat ggtctgaatg gtacgaccag aatcactcga
     4561 ataagcgcgc tcaaactgta gatcagattc tcgcaaaccg attcctaact cttgaatccc
     4621 tcgttcacct tcagcagtta agggagtatc gctccaacct tgcgcgcgac caatcgtgtt
     4681 aaacatggtc ttgccatgac gtatcaaata caatcgtact tttaccattt tccgtcctcc
     4741 gtttgcttct attatatcat gaatgataaa ataaaagcca cccatacagg cgacttttga
     4801 aggagattat tatgaaaaag tttaggagtt cattaaataa agatattaga tgaaaatcaa
     4861 attcaaacta attcagtgtt atctgtttca aataatatta ggaggtcttt atttaatgat
     4921 tatagtatac acatctaatc ttaaatagaa cttaaaattt ctcctatttt cttaattttt
     4981 aaaactatga attggtgctg ggatttgtcc accgcgagag atgaagtcga cagacgaagc
     5041 cccattaacc ctcataacgg gtgcagttcc taatagacca ccaaactcaa tcatatcgcc
     5101 ttcttttcct ttgggaatga tacgaacagc tgttgttttc atgttgataa caccgattgc
     5161 tgcttcatcc gcaatcatag ccgcaatggt ttcagcaggc gtatcttctg ggatggcaat
     5221 catatccaat ccaacagaac agatagccgt catagcttct agtttttcta aattaagaga
     5281 gccattttgc actgcagcaa tcattccttc atcctcagaa acagggataa aggcaccaga
     5341 taaaccaccg acttgattgc aggccatcac tccacccttt ttaacttggt cgttcaagag
     5401 ggccaaggca gccgtcgttc catgcgtgcc aactgtttct agccccattt cctcaaggac
     5461 acgtgccaca gagtctccaa ccgcaggggt tggtgccaaa ctcaagtcca caataccaaa
     5521 ctccacaccc agtctctcac tggccatttg accaaccaat tgaccgatac gagtgatttt
     5581 aaaggcagtt ttcttaactg tttcggctac tacatcaaag ctctgtccac gaactttttc
     5641 caaagcacgt ttcacaacac caggaccaga aactccgaca ttgatgataa catctgcttc
     5701 cccaacacca tgaaaggcac ccgccataaa tggattgtcc tcaacagcat tagcgaatac
     5761 aaccaacttg gccactccca tatctgaaag atttgctgtt tccttgataa ttcgtcccat
     5821 atctgccaca gccgtcatat taataccaga cttggttgag ccgatattga ctgacgagca
     5881 gaccttatcc gtctcagcca aagcgcgagg aatggaattg atgagaatct catctccctt
     5941 ttgataacct ttttgtacta aggcagaaaa accaccaata aagtccacac caatctcttt
     6001 cgcagcctta tcaagcgctt ttgccagaac cacgtagtcc gtcgcatctg tcgctgcccc
     6061 aatcagagaa ataggtgtca ccgatacacg cttattaacg ataggaattc ccaactcagc
     6121 cgcaatttca tcaccaacag ctactaaatt agccgccttt gtcgtaattt tttgatagat
     6181 tttctccgca gcacgattga tatctggatc gatacagtcc aaaagagaaa tccccatggt
     6241 aatggttctg atatcgaaat tttgctcctc aatcatggcg atggtttcag taacttgtct
     6301 aatatccatg ataacctcct agatattata catagcttcg aaaatcgctg cactctgaat
     6361 attgattttt acattcaaag tttgcccaaa agcttcaaat tcattacgaa gataggtaaa
     6421 atcttgcttt tcatcactag atacaatagc catcatcgta aaatattcat ccaagacagt
     6481 ttgagagata tcgtcaatat tcaatcctaa ttctgcaatt ttaccagaaa cacctgcaac
     6541 aattccagat ttatctttac caacaacagt tataatcgct ttcataagca aactccaatt
     6601 cttcttaaaa taagaaacct taacattaaa caggattctt ttcaaatagt atagcataat
     6661 tctaacgaaa atactcaaaa acgcctgctt acgaagttgt tcttaagaaa aaaacaattt
     6721 cgtaaacaag cgtctactat cccaacttat gatgagtgtt cccgctagga ccgaaatcct
     6781 agcggtagac cagagctaga ctaagagcac aagactccat catcataaca ctcaacaaaa
     6841 ttgatgattt tatactaatt cgacgatcgc cattggcgct gcatcaccac gacgtggttc
     6901 agttttaagg atacgagtgt atccaccgtt acgttcagca taacgaggtg cgatttctga
     6961 gaacaatttt tgaagtgctg tagtagaagt gtacttatca gttgcttcat catagttttc
     7021 agatgcgatt tcattacgta cgaaagcagc tgcttgacga cgtgcatgca aatcaccacg
     7081 tttacctaga gtaatcattt tttcaacagt tttacggatt tctttagcac gagcttcagt
     7141 tgtcacgatt gattcgttga tcaaaaggtc agttgtcaaa tcgcgaagca ttgctttacg
     7201 ttgtgagcta gtgcgtccta gtttacggta agccatgtat tcctccttta tttatctttt
     7261 aatccaagac ccaaatcaat gagtttgagt ttcacttctt ccaaactctt gcgtccaaga
     7321 tttcgtactt tcatcatctc tgcttcagat ttttctgtca aatcatgcac agtattgata
     7381 ccggcacgtt ttaaacagtt gtatgaacgc acagacaagt ccagttcctc aatcgtacga
     7441 tctaaaatac ggtcgtcaga ttcagtatca gcttctttca tcacttcagt tgacttagca
     7501 atctcagtaa gatttgtaaa caaatcaaga tgttctgtca aaatacgtgc tgaaagccct
     7561 aaagcatctt ctggaataat tgttccattt gtcaagattt caagggttaa tttgtcgaaa
     7621 ccatcattgc tacctacacg agcaggttcc acttgatagt tgacttttgt aactggtgta
     7681 taaatagaat ctacagcaag tgttccaact ggtgcattat cctttttatt ttcatcagca
     7741 ggtacatatc cacgaccact gttaacagtc atagtcgctt ttagagaaga accttcaccg
     7801 attgtaaaga gataatgatc tggatttaca atttcaatat cgctatctgt caaaatgtca
     7861 ccagctgtta cttcagcagg accttcaaca tccagttcga tgattttttc gtcttcaacg
     7921 tacgatttca ctgcaattcc tttaatgttc agaatgattt gcatcacgtc ttcacgaaca
     7981 cctggaactg tgtcaaactc atgtaacaca ccatcaatgt tgatagatgt cacagctgct
     8041 cctggtagag aagctagaag tacacgacga agagagttac caagagttgt accgtagcca
     8101 cgttcaagtg gttcgattac aaacttgcca taatctttat tttcatcaat ttttgttata
     8161 tttggttttt caaactcgat catttagtta ctccctctta aacgaaaagc agtgtaatgc
     8221 gatgattata cacggcgacg ttttggagga cgagcaccat tgtgtggcac tggagtcaca
     8281 tcacgaattg ctgttacttc aagaccagcg gcagcaagcg cacgaatagc tgactcacga
     8341 ccagaacctg gaccttttac agtaacttca actgatttaa gaccgtgttc ttgtgcagat
     8401 ttagcagcag cttcagaagc catttgagca gcgaatggtg tagatttacg agaacctttg
     8461 aaaccaagag caccagctga tgaccaagca attgcattac catgcacatc agtaatcata
     8521 acaatagtgt tattaaatgt agcgtgaata tgagcaatac cagattcgat attctttttc
     8581 acacgacgtt tacgtgttgg tttagccaag acttttacct cctatattat tttttcttac
     8641 cagcaatcgc aacagcttta cctttacgag tgcgagcgtt gtttttagtg ttttgtccac
     8701 ggacaggaag tccacgacgg tgacggatac cacggtatga accgatttcc atcaaacgtt
     8761 tgatgttcaa gtttacttca cgacgaaggt caccttcaac tttgattgca tccacttcac
     8821 gacggatagc atcttcttga tctgatgtaa gatcacgtac acgaacatct tctgagattc
     8881 cagcagcagc caaaattttc ttagatgttg caagtccgat accataaaca taagtcaatg
     8941 agattactac gcgtttgtca tttggaatat caactccagc aatacgagcc atgttttctc
     9001 ctttctatct tatccttgac gttgtttgtg ttttggattt gctgggcaaa ttaccataac
     9061 acgaccatta cgacgaataa ctttacagta ttcgcaaatt ggtttgaccg atggtcttac
     9121 tttcatttct tatccctcca agtttttcga ttatttaaag cggtaagtga tacgtccacg
     9181 tgtcaagtca tatggactca tttcgacagt aacacgatct cccgctaaaa tacgaatata
     9241 gtttttacga attttaccag aaactgttgc taaaatctga tgtccatttt caagttcaac
     9301 cgtaaacatt gcattcggca ttgtatcaac tactttgcct tcaacttcaa tcacatcgtc
     9361 ttttgccacg caaaagcacc tccataaatt tcgattcgat gcctctagac acagagacaa
     9421 caattataag tcagactatt tcagtataac atttgtagcg gatttttgca agtgtgaaaa
     9481 acgctttatt tcaaatttgt caatactttt tcaatatctg agaagacatc attgatatct
     9541 tgattacctt cgatgtcatg aaccaaacct ttggcacggt agtgagcaat gattggttct
     9601 ccttgagcaa tattaacatc caaacgacgt tttactgtct caggcttatc atcttcacgt
     9661 tggtagtaat cttcttcttt atagtcaact ggtgggttaa agaccttgtg gaaagtttct
     9721 ccagttacgc ggtggatgat acggccactc aaacgttcca aaaggctgtc agggttcact
     9781 tcaatattga taacaccttc tagttcaatg ccaagttcat tcaatgtttt gtccaaggca
     9841 tgagcttgtt caattgtacg tgggtaacca tccaataaga atcctgtttc tttaatatca
     9901 tcttgtgaaa gacgttcttt tacgattcca tttgtaactt cgtcaggaac caattcaccc
     9961 ttgtcaatat atgacttagc aagaacaccc atttcagttt gatttgccat tgcagcgcgg
    10021 aacatatcac ctgttgagat atgtgcaaca tggaattgtt ctacgatttt tgctgcttga
    10081 gttcccttac ctgcaccagg taagcccata atcaaaagat tcatgatttg atctccttat
    10141 tttattttta aatagaagca aaaagtcttt tcacccctat ttaaaaacaa aatagaagag
    10201 gggagatcca actcttacaa atgtcagagt ttaaacctcc actccctcag tatttactga
    10261 ttcagtaaat acttttattc tgttctgtcc atgaaaccaa catacttacg tttcaatagg
    10321 taaccttcca attgcttgat tccttcgata cctgtagaga taatgatcaa gagacttgtt
    10381 ccaccaaagg caacaacatc agaaagacca aatacatctt tagctgcaat cggtaaaatg
    10441 gaaatcacac caaggaagag ggaaccaaca gttgcaagac gacgaagaag tttagacata
    10501 tattcttctg tacctttacc aggacgaact ccatggatat aggcaccact cttttgtagg
    10561 ctctctgctg ctttttcagg attaatctgt acaaacgtat agaagaatgt aaagagaata
    10621 atcaacaaag catacatggc aataccagtt ggagaagtag ttgccagcat ctcttgtgct
    10681 acccttaccc aagcccaatc atgacctgtg gcactcaaaa actgaagaat agccgcaggc
    10741 gctgcagtaa tcgaactggc aaagataaca gggataactc cagcagggtt tacctttaac
    10801 ggaaggtaag agctagatgg agcaccttgt gcaaccttag tatattggat tggaattttg
    10861 tattctgctt gttgaacata agttgtaaag taaataatca acaatacagt aataatcaaa
    10921 atgattacga aaatgataga tgaagtgata cggctacttg ggacgttcac aaagtagtcc
    10981 acatagatgc cctgaatcat ctctggaatt gaggaaacaa tcccggcaaa gataatcatg
    11041 gaaacaccgt ttccgtatcc cttatctgta atttgctcac ccaaccaagt gacaatcata
    11101 ctaccagctg ttaagatgat accaatcgtc agaaaaactt gtggagttaa agcagtttta
    11161 atcaattgag ctccagccaa ggtattaaaa ccagctgtaa tcccgataga ttgcacaaaa
    11221 gcgagaacta gagcaatata acgagtagct tgattcaatt ttcttcgacc tacttcccct
    11281 tgtttacccc actctacaaa cttgggtaaa atatccattt gcaagagttg gacaacaata
    11341 gaagcggtga tatagggact aactcctagg gcaaaaatcg aaaagttttt tagggcattc
    11401 cccgacacca agctcaacat gtttaagaag gataatccac ttaaagcatt caagctattg
    11461 gcattcacac caggaactgt aatgctagtt ccgatacgaa agaccaaaac gataaaaatt
    11521 gtaaataaaa tttttgatcg aacctgcttg actttaagag cttctcttaa taatttaaaa
    11581 aacataggtc acctctctta gatgacttct actgaaccac ctttagcagt gatagcttct
    11641 tcagctgatt tagagaattt agctgctttc acagtcaatt tcttagtcaa ctcaccgtta
    11701 ccaagaattt taatacctga cttttcagct ttaacaattc ctgcttcgat aagaacaact
    11761 ggagttactt cagcaccatc ttcaaagacg ttcaattggt caaggttcac aattgcgtat
    11821 tctttagcgt tgatgttagt gaatccacgt tttggaagac gacggaacaa tggagtttgt
    11881 ccaccttcaa aaccaaggcg aactccgcca ccgctacgag ctttttgacc tttttgacca
    11941 cgaccagatg ttttaccgtt acctgatgaa gtaccacgac caacgcggtt acgtacttta
    12001 cgagaacctt ctgcaggttt caattcatga agtttcattt ttattttctc ctcttttgta
    12061 aaatgctagc gccgataagg gagaaaaggt tgtctccccc atcaactcgc ctatacgatg
    12121 tcatcataga tgactatatc tagttttagg ggatggtaca gtgcacatcc cctaaaactt
    12181 cattagttta cttcttcaac tgttactaag tgagatactg ctgtgatcat accacggata
    12241 gcagcgttat cttctttaat aacagagctg ttcaatttgc caagtccaag tgctacaaca
    12301 gttttacgtt gtgatggaat gcatccgatt ggagacttag tcaaagtaat tttaatttga
    12361 gccattttat cccctttctt atgccaaatc agaaactgaa ataccacgaa gggcagcaat
    12421 ttcttcagcg cgtttcaatt gtttcaaacc ttcaacagtt gcacgaacaa tgttgattgg
    12481 agtgttagaa ccaagtgatt tagatgtaat atctgccaca cctgccaatt ccacaacggc
    12541 acgaactgca ccaccagcgg caactccaga accttctaca gcaggtttca acaatacttt
    12601 agctccaccg aattctgaaa gaacttcgtg tgggattgtt gttccaacca taggaacttc
    12661 gatcaagttt ttcttagcat catctactgc tttacggatt gcttctggaa cttcttgagc
    12721 tttaccagta ccaaatccta cgcgaccatt gtggtcacca acaacaacaa gagctgcgaa
    12781 acgaagacga cgtccacctt taacaacttt tgtaacacgg ttgacagcaa ctacgcgttc
    12841 ttctaattca actgcattgt ctttaaatgc cattttctag tgtcctccta ttagaatttc
    12901 aatccgtttt cacgagctgc atcagccaaa gctttcacac gtccgtgata tagatatcca
    12961 ccgcggtcga acaccacttc tgaaatacct ttagcgtttg cacgttctgc aacgagttta
    13021 ccgacagcaa cggcttgttc agttttagtt ccttttgaaa cttctttatc aagagttgaa
    13081 gcacttgcga gcgttacacc cgctacgtca tcaatcactt gagcgtagat gcctgtatta
    13141 gaacggaata cgttcaaacg tgggcgatca gcagttccag agagttttcc gcgaacgcga
    13201 cggtggcgtt tttggcggag tttgttttta tctggttttg aaatcacagt tttcacctct
    13261 ttagttttaa atcgtgtgct atgcacaaag ttggaaaata ggttggtggt tgatgatcaa
    13321 ccactcaaca ttatttacct gttttacctt ctttacggcg aacgaattca ccaacgtaac
    13381 ggataccttt acctttatat ggttctggtg aacgaaggct acgtacgtaa gcagctgttt
    13441 gaccaactac ttcttttgaa attccgctaa caacgattgt tgttgggttt ggaagttcaa
    13501 aagtaattcc ttctggagct tcaacttcgt ctggatgaga tttaccaaca gccaaaacaa
    13561 gtttagatcc ttgaagctgt gcacggtaac caaccccacg catttcaagt tctttcttga
    13621 atccttctga tacaccaaca accatgttgt tcaaaagggc acgagtagtt ccgtggatag
    13681 ttttcatttc ttttgaatcg tttggacggt gaagagttac ttcagtacct tccacacgga
    13741 tttcaatatc ttttgagaac tcacgagtaa gttctccttt aggtcctttt acagttacaa
    13801 cgttgtcatt gttagcgagt tcaacaccag caggcaacac gataacttta ttaccaatac
    13861 gtgacatgtt tattttctcc tgttaaattg tcaggccaaa acggccagtt ttcacggggt
    13921 tcagatactt atttagttca agagctgaac tgaacacgct ctaagaactg tgcaaaatag
    13981 ataagttggc ttgtttgtaa acaaacttct ccaacttcct aattttgctt tgttctttac
    14041 gagctttgta tcttgatttt accaaacgta agcgataacc tcaccaccaa cattcttttg
    14101 gcgtgcttct ttatcagtaa gcaaaccttc agaagttgaa aggatggcaa ttccaagtcc
    14161 gttaagaact tttggaaggt cttcacgttt tttgtagaca cgaagtcctg gtttagaaac
    14221 acgtttcaag ttagtgataa ctttctcacc atttggtccg tatttaagaa atacacggat
    14281 gacgccttgt ttgtcatctt caatgatttc aacgtttttt acaaaacctt cgcgtttaag
    14341 gatttcagca atcccttttt tgatgtttga tgcaggtact tcaagtactt cgtgtttagc
    14401 ttggttagca ttacgaatac gagttaggaa gtctgcgatt gggtcagtca taaccatttt
    14461 gtttttctcc tcttactagt agtttgcaag ttgcacttgc tagttaatac gtgatacaaa
    14521 gagcgtaaca gcacgagcaa aaatagacaa tttaatgcag aagcaatgct ccaaagcaaa
    14581 ttggtctttt ttgccaaagc tgtagctcgt gttcaaattg gcaagcccaa ttgaacccgg
    14641 gctaaactct gtgtgaaaaa gataaacttt cctagaaact ttagtttctt catcaagttt
    14701 cctattttta cttggagttt tgacgctctt gatatcttaa attaccaaga tgcttttgtt
    14761 acaccaggaa tttgaccttt atgcgcaagt tcgcgaaaag cgatacgact cagaccaaat
    14821 ttgcggtaaa ctgaatgtgg gcgccccgta actctacaac gattatgtaa acgagtcggt
    14881 gaggcgttgc gaggtaattt agataaacct tcgtagtccc ctgccgcctt taatgcagca
    14941 cgtttttcag cataacggtc aacaattttt tggcgtttag cctctctagc taccattgat
    15001 tttttagcca ttagatttac ctcctatatt attttgcaaa aggcattcca aggcctgtaa
    15061 gcaatgcacg tgactcttcg tcagtgttag cagttgttac gataacgatg tcaagaccac
    15121 gagttttgtc aacgtcatcg aagttgattt ctgggaagat taattgttct ttcacaccaa
    15181 gtgtgtagtt cccacgtcca tcaaatgatt ttgttggaac accgtgaaag tcacgtacac
    15241 gtggaagtga aactgatacc aatttatcca agaattcgta catacgttca ccacgaaggg
    15301 taacttttgc accgatcgca acaccttcac gaagacggaa gccggcgatt gattttttag
    15361 ctttagtgat aagtggtttt tgacctgaga taagtgccaa ttcttcagca gctttttcaa
    15421 ggcttttagc gtttgataca gcttcaccaa cacccatgtt caaaacaatc ttatctactt
    15481 taggcacagc catcactgat gagtagttga attgttctgt caaagcagga actacttcat
    15541 taagatattt ttcttttaaa cgatttgcca ttatacttct cctttccttc gtgattaatc
    15601 aagcacttcg cctgattttt tgttgtagcg aactttttta ccgtctacaa atttgtatcc
    15661 aacacgacca gctacaccat ttttgtccaa aacttgaacg tttgatacgt ggatagctgc
    15721 ttctttctcg atgataccac cttgaggaag ctcgttagtt ggacgttggt gtttcttaac
    15781 aatgttaaca ccttcaacga taactttgtt tacttttgga agggcagtaa ggacaacagc
    15841 ttctgttccc ttgtctttac cagcgattac gcgaactttg tcgccttttt ttacaaacat
    15901 taggtttctc cttgattttt cttacgccca taagggcacc ctagcttgaa gctaggggac
    15961 tagtttgttc ctaaaaatta aagtacttct ggagcaagtg acacgatctt catgaagcca
    16021 ccttcacgca attcacgtgc aactgggcca aagatacgtg ttccgcgagg agttttgtct
    16081 tcacggatga taactgctgc gttttcgtca aatttgatgt atgaaccatc agcacgacga
    16141 gcacctgatt tagtacgaac gataactgct ttaacaacgt cacctttttt aaccgcacca
    16201 ccaggagtag cttgttttac agatgccacg ataacatcac cgatgtttgc aaatttacgt
    16261 cctgaaccac caagaacttt gatagtcaag atttcgcgag caccgctgtt gtctgcgact
    16321 ttcaaacgag tttctgtttg aatcatttca gttttctcct ttcaggtttg attagatgat
    16381 gaccgcttct tcaacaactt ctacaagacg gaaacgtttt gtagctgaaa gcgggcgagt
    16441 ttccatgata cgtacgatat cgccttcttt ggcaacattg ttttcatcat gagctttgta
    16501 ttttttagag tagttaatac gtttaccata gactgggtgg ttacgttttg tttcaactac
    16561 aactgtgatt gtcttgtcca ttttgtcaga tacaacacgt ccaacaagaa ctttacgatt
    16621 attgcgttcc attgaaattt ctccttccct agtctattat ttcgcttcag attgaactgt
    16681 tttgatacga gcgatttgtt ttttaacttc tttcaagcga gctgtttgtt ccaattgacc
    16741 agtagcagct tggaaacgaa gttcaaacaa ttcttttttc aattcgtttt cgcgcttcgc
    16801 gagttcttct tgagaaagac cacgaagttc tttaacaaat tcttttactt cattaagttt
    16861 catgccttct ccttattctg cttcacgttt tacgaattta catttaactg gcaatttgtg
    16921 gctagcaagt cgaagcgctt cacgtgcaat ctcttcagat acaccagcga tttcgaacat
    16981 cactttacca cgtttaactg gtgctaccca accttcaggt gcccctttac cagatcccat
    17041 acgcacaccg atagctttag cagtgtatga tttgtgtggg aagattttaa tccaaacttt
    17101 accaccacgt ttcatgtaac gagtcatggc gatacgagca gcttcgattt ggcggttagt
    17161 gatccagtgg ctagttgtag cttgaagacc gtattcaccg aatgctactt cttttccacc
    17221 ttttgcttca ccgcgcattt ttccacggaa ctcacgacgg tgtttaacac gtttaggtac
    17281 taacattggt tatttacctc ctttagtgtt tttacgagct ggaagaactt caccacggta
    17341 gatccatact ttaacaccaa gtttaccgta tgtagtatct gcttcttccc aagcgtaatc
    17401 gatatctgca cgaagtgtgt gaagcggaac agttccttca gagtatcctt cagcacgggc
    17461 gatatctgca ccgttcaaac gacctgatac ttgagttttg attcctttag ctccagcacg
    17521 cattgcacgt tggattgctt gtttttgtgc acgacggaaa gcaacacgtt gctccaattg
    17581 acgagcaatt ccttcaccta caaggtgagc atccaaatca ggttgtttga tttcgatgat
    17641 gttgatgtgt acttgttttc cagtcaattt gttaagtttt gcacggagtg catcaacgtt
    17701 agcaccacct ttaccgataa ccatacctgg tttagcagtg tgaagtgaaa cgttaacttt
    17761 gtttactgcg cgttcgattt caatagttga aactgctgcg tcagcaagtt ctttttgaac
    17821 gaatttacgg attgcaagat cttcatgaag gtaatccgcg tattcttttt cagcatacca
    17881 tttggcatcc caatcacgga tgatgccgac acgcatacca attggatgta ctttttgacc
    17941 cacgatttta cctccttatt tttctgcaac agctacagtg atgtgagctg tacgtttgtt
    18001 gattggtgaa gctgaacctt tcgcacgtgg acggaaacgt ttcatagttg gtccttcgtt
    18061 tgcgaatgct tcagatacta ccaagttagc tttatccaaa ccaaagttgt tttcagcgtt
    18121 agctacagct gagttcaaaa ctttcaagat gatttcagca gctttgtttg gagtgaatgt
    18181 caagattgcg attgcatcgg ctacgctttt accacggatg ttatcaagaa caagacgtga
    18241 tttacgaggt gaaacacgta ctgtacgagc cattgcttta gctgaagtaa tttctgccat
    18301 ttatgttctc cttattttct acgtgttttc ttgtcgtctg cagcgtgacc tttgtaagta
    18361 cgagttggtg caaattcacc aagtttgtgg cctaccatgt cttcttggat gtaaacaggt
    18421 acgtgtttac gtccgtcata aactgcaata gtgtaaccaa tgaaacttgg gaagatcgtt
    18481 gatcgacgtg accaagtttt aataactttt ttcttttcgt cgttagcttg agcttcaact
    18541 tttttcatca aatgctcatc gacgaaaggt ccttttttaa gactgcgtcc catttttata
    18601 ttttctcctt taaatgttgt accacagcgg cttgcactcc tatggagcgc taccgagctg
    18661 gcggatttac tagttgctta agcgactagt ttaatattat ttctcgttgc gacgacgaac
    18721 gataagtttg tcagatttcg ctttcttgtt acgagtttta agaccaagag caggtttgcc
    18781 ccatggagta gatggtgctt tacgaccaac tggtgcttta ccttcaccac caccgtgtgg
    18841 gtgatcgtta gggttcatta cagaaccacg aactgttggg cggatacctt tccaacggct
    18901 acgtcctgct ttaccaaggt ttacaagtcc atgttgttcg tttccgacaa caccaactgt
    18961 agcacggcaa gttccaagaa tcatacgaac ttcacctgat tgaagacgaa caagaacata
    19021 tttaccttca gaacccaata cttgagcaga tgcaccagca gcacgtacca attcaccacc
    19081 acgacctggt ttcaactcga tgttgtgaat caaagtacca actgggatgt tagcaagtgg
    19141 aagagcgttt ccgactttga tatctgcttc tggacctgaa acgatacgtt gacctacttc
    19201 aagacctttt ggagcgatga tgtatgcttt cacaccgtca gtgtagtgta caagagcgat
    19261 gtttgcagaa cggtttggat cgtactcgat tgttttaaca actgcttcaa cgttgtcttt
    19321 attacgtttg aagtcaacca aacggtagaa acgtttgtgt ccaccacctt ggtgacgaac
    19381 tgtgatacga ccgttgttgt tacgaccagc cttgctcttc aatgcaacaa gcaatgattt
    19441 ttcaggagtg cttgttgtga tttcagcgaa atccaaagaa gtcatattac ggcgaccgtt
    19501 tgttgttggt ttataaacac gaattcccac gatatttcct ccttagatta ttcagcttca
    19561 gcagcaaaca actcgattgc tttagaatca gctgtaagtg tgatgatagc ttttttagtt
    19621 ttgttagtaa aaccagtgta acgtccaaca cgtttagctt ttggttttac gttgattgtg
    19681 ttaacattgg caactttaac accttcgaaa gcagcttcaa cagcttgctt gatcaaaagt
    19741 ttgtgtgcac gagtgtcaac ttcaaataca tatttccctg cttcaagttg agccattgag
    19801 ctttcagtga tgacaggttt tttgataaca tcatacaaat tcattatgca agaacctcct
    19861 cgattttaga gatagctact tgtgtgacaa gaagtttgtc gctatttgcg atgtcaagaa
    19921 cacttgcagt tgtagcagtt gcaactttca cgtttggaag gttacgagct gaaagagctg
    19981 cgaattcatt tccttcttca aggataacaa gaactttaga atcgatgctc aatgctgcaa
    20041 gaacttttgc aaattcagca gtttttggag ctgtaaatga aagagcgtct acagctacga
    20101 atttgttttc agcaactttt tcagagtaaa ctgatttaag agctaggcga cgaacttttt
    20161 gtggaagttt gtagccgtat gaacgtggag ttggtccgaa gacaacacca ccaccacgcc
    20221 attgtggtga gcggatagaa ccttgacgag cacgtccagt tcctttttga cgccacggtt
    20281 tgcgtccacc acctgatact gcagagcggt ttttaacagc gtgtgttcct tgacgaaggc
    20341 ttgcgcgttg gctgatgatt acatcaaaca caactgattc atttggttcg ataccaaata
    20401 ctgcatcgtt aagaacaact tggccagctt ctttaccagt ttggtcaaat aatgttacgt
    20461 ttgccattgt gactgatttc ccctttcttt attatttacc agctttaact gctgatttga
    20521 tagtgataag agatttctta gcacctggta cgttaccttt gataaggata acgttctttt
    20581 ctggaacaac ttgtacaact tcaagatttt gaattgttac gcggtcacca cccatacgtc
    20641 ctgcaaggtt tttacctttg aatacgcggt taggtgcaac aggccccata gaacctggac
    20701 gacggtggta acgagaaccg tgagccattg gtccacgtga ttgtccgtgg cgtttgataa
    20761 caccttggaa acctttacct ttagaagtac cagttacatc aacaacatct ccagctgcga
    20821 atgtttcaac tgtgatttca gcgccaactt ccaagccttc aacgtttttg aattcacgaa
    20881 tgaagcgctt aggagccgtg ttagctttcg ctacatgtcc tttagcaggt ttgttgctca
    20941 atacttcgcg tttgtcatcg aaaccaactt ggatagcgtt gtatccgtct gtttcaacag
    21001 ttttaacttg aagaacaacg tttggagttg cttcaataac tgttacaggg atcaattcgc
    21061 cagcttcagt gaagatttga gtcattccca cttttttccc taagattcct tttgtcatga
    21121 gaaaatagtt ccttttctat attttttatt caaaaagttt ttaacgagcg ttttttatgc
    21181 tcaagtctaa gatacaaagg gcgtcaaact caacttgaaa ataggaaact gacgcagtgt
    21241 ctagcagaca ctaggaagtt tatctttttc acaccgagtt tagcccgtgt tcaattagat
    21301 tgaaacacca gcctctgctc ttttatattt tagattaaag tttgatttct acgtttacac
    21361 cacttggaag atccaatttc atcaaggcat caactgtttt ttgagttggg ttaacgatat
    21421 cgatcaaacg tttgtgtgta cgcatttcaa attgttcgcg agagtctttg tatttgtgag
    21481 tcgcacgaat gattgtgtag aggctacgtt cagttggaag tgggattgga cccgcaactt
    21541 gtgcacctgt acgagtagct gattctacga tttttgcagc cgctgtgtca agcgtacggt
    21601 gttcgtaagc tttcaaacgg atacggattt ttttgtttgc catctttttc tccttttcgt
    21661 ctatttaaga taataggcta gctccacaag aaaaccgaca cggtgttgcg tggcaatgca
    21721 accgagcgtg tcgcaacctc ttgcatcaaa actaaggctg taatttacag caccataata
    21781 gaataacaca aatcccctgc gattgcaagg gatttgttag gttttttcgt ttttttggga
    21841 tgaaactgtt ttcctacttt ttctttctaa aattagggat tttcttctat atttcttcta
    21901 tacataagtt aagtcctctt gacatcaaat ttatcttctg attgatccac taagatatcc
    21961 gccttagact cagtttcttt atagtatcgc agatactgct cccgtctcat ctgatggcta
    22021 gccaatataa aggatgcatc acgatttctc acagtcgtat ctctagcaag gcgtcgcttt
    22081 aattcggtct cctcatccgt gtagaaacag atggtttttt caaatagttc cttgggtaga
    22141 aagccaacag acatcccttc gacaatcaaa attggtttgg ttccagacaa gacctcacca
    22201 gccttccaag gttcttcaat tgtcaagaca tccatacccg cctgcaaggc aaggatatct
    22261 ctctgcaaac tctccagttc atgcgccact ggcagactgg ctgtcacctt ttgattcggc
    22321 gcgtccttgg gtactaccag atggcgtcct gaggtgatat aaggatctgt ctctagcaaa
    22381 tttactgtag tagaatctaa agcttggtac aattcctgtg caaaggttga tttacctgaa
    22441 gctccatgac cgtatattcc cagtgtcctg actttccccg tctcgatctc tgagactagt
    22501 tggtctacta agtctttttt cttcattctc tcttcacctg ttcatagctt tcttttccgt
    22561 cattgagctt gtcccaaatc actacttgcc cacttttgag cgatttttgc acatcgataa
    22621 ttcgttggtt agacgaacct cgaaactgga gcataagatt tctcttagtt cgatcatatc
    22681 ttccatcgac aagaatgtca atcagtgaca agagttccag tttatctgga gtttccaaca
    22741 tcatttcttc ccaagtgtag ccggtccagg accaaatgtc cttgtctggc aattcctttc
    22801 ggatgcgttt aactagaggc aagagaatgc cagtattaag aaaaggctcc cctcccagca
    22861 aagtcaagcc ttgaacatag ggttgggcaa ggtctgccat aatctgctct tctaattctg
    22921 ctgtataggg aatgccagca ttaaaagacc aagtcgcaac attataacat ccctcgcagt
    22981 gaaacataca gcctgataca tagagagagt tacgcacgcc ttcgccatca acaaagttaa
    23041 aggccttgta gtcaatgata cgaccttgac taagttcctc gcttttccat tcttgtggtt
    23101 ttggattatt cattcgctac ctctatccaa taacgctcga ctccattgtg agcatcctca
    23161 aatattccac catttgctag aatgactgct ctgctagcag gattattcac gctacaggtc
    23221 accagagctt tcttgatgtt cttttcctta gcaacttgca agccctgacg gagagtctct
    23281 tttgcataac ccttgccttt ttcagatgga cgaatggagt agccaatgtg gccagcataa
    23341 ttcagtaacc tctcattcag tctcaatcgc agattcaaaa atcttagagc acaacctgat
    23401 tcaccaaaca atacgaactg tatcgatgga acgcgatttt caggcaagtt tattcccatc
    23461 tctttttgaa tatttgtttc caaccactct tcatacacaa agttctctgt atcccaaaat
    23521 cctccatcat gggctgattg gctcttttca aactctgtca tcatatctaa aacagctttc
    23581 ttatccgcta atcttggtct gcgtaattcc atccttacct cccactatga gaaaagcgag
    23641 agctggctct cacttttatt ttttcttgtt cttgtttaaa aattgttctc tgaagctaag
    23701 caagcgttct ttcttaccag ctccaccttt agagtgcttc tcgtgataac gggtcacttg
    23761 tgcgcgcccc ttatcgtcta gttgatattt tcccatttca tttctttcta atttgttact
    23821 tgatgcccag ctattttaat cgttgaacca ttcatatgtt tgacacgcgc agcgatttcc
    23881 ttgtgacgcc cgttgaccat cggtcttgct tgaggattac ctaggtagcc acaagttcgc
    23941 ttcaccacat ctactgtttt agggtcgcta ttgccacagt ttggacaagc aaaccctctc
    24001 tcagttggtt caaaatcccc ttcaaagtca cacttgtagc aacggtcaat cggagtattg
    24061 gtgcctagat agcctacacg gtcataagca taatcccaga cagcttccaa ggcctttgga
    24121 ttttgctgaa ggactggata ctcacaataa tggatgaaac cacctgacgc acctgcttct
    24181 ggatagactt tctcaaagtc caatttttca aacggtgttg gatttttacg aacatcgtag
    24241 tggaaagagt tggtgtagta ttccttgtct gtgatatcag gaatagagcc aaacttgtct
    24301 atatctagtc ggcagaaacg gtctgtcaga ctttcggatg gtgttgagta gatagagaaa
    24361 tggtagccat attggtctga ccactcttct acacggcgtt tcatatcgtg aatgatgtct
    24421 agcgtgaatt ccttagcatc tggattactt tcccagctgt taccaaagaa aactgtcgct
    24481 acttcataca agccgatata gcccagcgaa acggtcgcac gacgattctt aaagagctgg
    24541 tcaacacttt cttctttacc tagatgatgg ccaaaagcac cgtactgata aaggatagga
    24601 gcattcgctg gtgtcgcctc tttagtgcgt tcgacacggt aaacaagagc atcttctgcg
    24661 atattcattc gctcgttgaa gatttcccag aacttattca tatcaccttc agactcaaga
    24721 gcaatacgag gcagattaac cgtcacaaca cccagattca tgcgacctga attgacttct
    24781 acaccatttt catccttcca tccttggagg aaagaacggc aacccatagg caccttgaag
    24841 gatcctgtca agtcaacaat cttatcgtaa gataaaacat ctggatacat ccgtttggtt
    24901 gcacactcaa gagccaactg cttgatgtcg tagttgggag ttccttcctc taaattaagg
    24961 cctcttttca gagtgaaaat aagtttagga aagatagccg tacggtgttc tgaaccaaga
    25021 cccttgatgc gaatgtttaa aatagctttt tgaatttctc gttcaaaacg actggttccc
    25081 agaccaaaac ctagcgaagt aaaaggtgtt tgtccatttg aagtgaagag agtattgatt
    25141 tcatactcaa gagattgcat ggcatcgtag atgtcctttt gcgttttctt ccaagcgtaa
    25201 tcttcccatt tttcaggcaa tacccactct tctgcatctt tgagatgttt ttgatagttc
    25261 ttctctgcat aaggcgccaa aatttcatcg atacggtcag ctgaacagcc accgtactgg
    25321 ctagaagcaa cgttggcaat gatttgagaa atctgtgctg tcgcagtctg gatagactta
    25381 ggactctcta cctctgcatt tccaatctta aaaccatttt ccaacatacc cttaaaatca
    25441 atcaaacagc agttggtcat aggggtatag ggactgtagt ccaaatcgtg atagtggata
    25501 tccccctttt ggtgggcatt ggctacgtgc ttaggaagca tttgcagtcc gattgatttc
    25561 ccaacaatcc ctgctgtcaa atcacgctga gtgttaaaga catcactgtc tttattagca
    25621 ttttcattga caactgtctg gtctttgttg agaagtttat gaatactaaa gttgatatcc
    25681 gtcgcttttg agcgctcaaa atccctctgt gtccgataag taatatactc ctcagccagc
    25741 gcatattctt tggcttcaag gagttcatgt tctacgatat tttgaatttc gtaaatctta
    25801 attccctgtg gaaagcgact atgaatttct gtaataattc gctcagtcag atcattgagg
    25861 cgtttttcaa ccaggggtgt cacatccata accttgtcag ccgccttgtg gagagccttg
    25921 tcaatcttgt ccacatcaaa tacaacacgt ctcccatctc gtttctcgac gaagagagtt
    25981 ggcaaaattg taattttttc ttctagtgca atcatatgca tgctcttttc taaaatgttc
    26041 tttctaaaaa gtattatacc actctaaaaa gaaaaatcaa tatcttgtgt taaaaatcct
    26101 aaaattttta aaaatacaca agatattgat ttggttattt aattttatag actttaaact
    26161 ctgagtaatc agtctttact tgttggtaat tttcttttaa gagtgtttcc acttcagacc
    26221 agactaccac cttgtcattt accacaatca ccttaggttg tttttctttc aagtcattaa
    26281 gtaacttatt acgattttcc tcagttgctg tatagtgcaa cggggacaaa atcgccgatg
    26341 gcgacaagcg ctcactctta cgataaagag tcgcagtatc atcccatgca tagatagcat
    26401 cttccgtatt tgtctcctct tttaccaaat cagcaagacg attacgttct tgataaggta
    26461 ctggataaag gacaaacttc atcaaaaaag gaacagacaa aagatagaca aacccaaaaa
    26521 ctagcagata tagatttcct ttgaagaaac gactccatag gctaactggc gtttctcttc
    26581 ggcttcttct agcactacgc cccctctgtt gactcttaat attggttacc aataaaagta
    26641 ggaaaacagg gaagacgacc atcaaataag aagcgtgcag aggctcttgt gaaaagaaaa
    26701 gtaaaatcaa acctaaaatt accacaagac ttgcaggaac cgaaatgacg tatagtttaa
    26761 agggtttgga ttgaaagaga cctaaaaaga tacaaaccac aaatcccaaa ccaaggaaca
    26821 acaagccata aaaaaacata ttctctaaaa ttttagaagt aaaatcaaag cgaatactgt
    26881 caataggata acgaatacca ctaatcgcat ccccaaaact tcctgttgca gcactatagt
    26941 aggcagttgg ataaaagaca agtgaaaaac ctaaagccac tgcaagaaac tgataaaacc
    27001 catgcgcaaa gcgtctatgc ccaaggttaa agaccaacaa gcctaaactc actacagcaa
    27061 taaacaggag cgatgataag ggagcaaaga aaaatccgcc tgccaaagct agcccaatcc
    27121 gtacaaatcc cttatcatgg cttggattgc ttaggtaagc cgcaactaaa ctaaaggctg
    27181 cgaataagaa aggaagcgct aaaagagtcg catagcctcc accaaaagca agacctgtaa
    27241 ctagcatgta aaaaatagtc accagatgtc cagcttggtc tccttgctct gtcaaggtgt
    27301 ccgctgatct aaaaaggaaa aatcctcctg ctaccaaggc taaccactca aagatggcaa
    27361 agaaaaatcc gccctgactc acgtaagtca gcaaataata aagcaaacct tgacttccat
    27421 aatagtcgct gtaaatcttc cctgtctgat gaagcgccca acctgcataa aaatcctgca
    27481 cttcttgtgc actcattaag tcgagtaata gcggtactcc tagagttatc cccgttacaa
    27541 gcgtactcca tagtaaaatt ttcaccaaag gaagacgact tgattcacga tgatgcgatt
    27601 cttgttcgat ttggtattct agaggttcac gattctcctt atgaacttct tctactctac
    27661 catacacact catatcgttt ctcctgttca atttatctgt cttagtatat caaaaagtcc
    27721 taggattgtc agcttgcgat ggctgtttga gtgatttttt gcatagtttc acaaaggttt
    27781 caatttcccg aaagcggtgt aaatgtgtct gtttgaactc aatcatatca ttcacttctt
    27841 ccttagttag catgaattcc cctcctcttt ctttctattc gtaaaagaca agaaaaatag
    27901 gtctggtttg tatcctaagt tctagatttc ttataagata ggggcgaata gctgggcgat
    27961 ttcttttaca agcttttgat accagctcgt tttgatagaa tgaggataga cttcctgaga
    28021 atctgcaaat atatttcgaa aatctcgggc gatttccctt atagaaggag ttttatagag
    28081 taagactgca ttttcataat ggtgtaccaa gctccggtag tcgagattga ttgtccccac
    28141 caccgcaaaa tcttcgtcta ccaacatctg cttactatga ataaaacctg gactatactc
    28201 ataaatccga acaccagcag aaagaaggtc gggataagct cctctcgtga ctaactgaat
    28261 gaacttctta tctggtatgt aaggggtgat aattcgaaca tcgacccctc tcatagctgc
    28321 atttttgatt gtctctgtta aatcataatc tataatcaaa taaggtgtcg taatatagac
    28381 cgattctgtt gcttgattga ttaaactctg ataaactttt ttccctacct gcgctcgaaa
    28441 aattggcttg ggtccacttc cgtatggaat ggttaaaccg tcactcggga tagaatgatt
    28501 ttctaaatga tattgatcaa aatcactaat ttctcctcga ttaatgtacc aagtggtcaa
    28561 aaataagcgt gtcagagctt ttactgctag tccgtctaag cgaattccac tatccttcca
    28621 ataaccaaat ctctcgacgt ggttaatgta ctcatctgcc agattgaccc caccagtata
    28681 ggctatctga ccatcaacaa tcaatatttt tctatgatct ctgttattat aagccactgt
    28741 caaacgagga ataactttat tgaatttatg ggcctcgatg cccagctgac gaagtcgatg
    28801 tgcataatct cctgttaaag tagccataca gccgatatca tcatatagca tcttaacctc
    28861 tacaccctga gctacctttt gctctagtat atctagtatg cgattccaca tcaaaccttc
    28921 ttctataatg taatattcca agaaaataaa tttctcagcc tttttaagat cttctaccat
    28981 ctttttccac atagcttctc cgttaggaaa aaatgtagag gcagtttgat catagatgtc
    29041 agcattggta tccatactca ataaggactt gatgactcca taagctgcct tgtcactttc
    29101 ttttaatttc tcttttagta gctggctatt tgcttcttgg aaatgcatag agcctagctt
    29161 cttcagttgt ttgatttctt ttttggacaa tcgcctttca ccaaacatca gatagagcaa
    29221 gggaccaaat actggcacaa aggctactaa caaccaggtt accttattct caggagtcgt
    29281 attacggttg actatcgaaa tgattgtgat gatactcagt agaatgagga cagtaatcca
    29341 taaaatggga gccatgcgtc ctaaataaag aaagagacca aaaacaagac aaagttcaag
    29401 tagcataatc gaaagactaa agccatactt ggacatcaat aattgaaatt tcctatattt
    29461 catatcccct ccttgatagt ttgaatgcta aaaacaagta attgatacta gtataacgca
    29521 ggtattcggt agaaggcaag tatttatgat tattttctag gtaaaagtaa aaaggctgaa
    29581 aaatccagcc ctttctactt gcatagtctt atttagttaa aacgatttgg tccgatgggt
    29641 ctctatccat atcatctacg acgatttgat taccgtttac agtgtaaatt ttgacatcat
    29701 cgccaataat catgcgttga tttgctgaat caaatgtaac ctgtttgact ttctgatccc
    29761 cgtcagattc gagctcagtc catgtaccag tcgttccagt taccactaaa gtgatacggt
    29821 ctccgtcatc ctgaccagta taagtcccat caatatcggt cggttgagca acaggttggt
    29881 tttgtactgg agccgcttga ttttggttac ctcctgcatt tgcagtattg gtattctgtt
    29941 gagcaggctg ttggacctgc tgagcaacag gctgttgaac ctgttgagct ctttgtgaac
    30001 aagctactaa gagactaaaa cttgctagag aagcaagaga aagtgtgaat ctttttaatt
    30061 tcatgatgaa tttcctttct gctaccaatt tagagaaatt ttctctaacc agcaattccc
    30121 ctagtataac aagttcaaaa aagggggtca atttatctgc tcacggtcca gcaggtagcc
    30181 ccgtacttct gagataaaat agagagaccc tgtaacgaac agcaagtctt gagcgtctgc
    30241 cctttcttcg aaactgctga taaattctcg gtaagaggga attacatggt aacctgttac
    30301 atctgtctcg tccaaagacc cttgatagtc aaagccggtc attttgagtt ccacttgagg
    30361 taaattttca gtcagataac ccaacatccc ttgataatcc ttacgtttca aggatccaaa
    30421 gaggatttga ggtcgatagc cttcctgctc tttttctttg ataaactcaa ccaagcaagt
    30481 caaggcaggg aggttatgag caccatccaa gtaaatctgt gggcgaatac gctccaagcg
    30541 accagcccaa tgggtctgtt ccaaggcctt tcttacagcc tgttcatcaa tagcttcctt
    30601 tctttctccc ataaaaagaa gaaaagtttg caacgccaag gccgcattct cctgctgata
    30661 ggctccttct aagcctatgt tcagctgtga aagatttagt aaagagcttg aaaaatcacc
    30721 attcagcact gaaaaatctt gacctgcctg ataaaggtca acagctaaag attcggcttt
    30781 tttctgacag ataagcctag cttctggagg caattttgca atcactgcct ttttaccagc
    30841 cttgaaaata ccagctttct gctctgcaat tgcttctaga ctatcaccca aggtttcttg
    30901 atggtcaagc ccaatggagg tgatgacagc aagctctcca gttaccacat tggtcgtgtc
    30961 aagtaagcca ccaattccca cttctaataa aaccaaatcc acctcctgct ccctaaaata
    31021 aagaaaagca accaaggtca gcaattcaaa aaaggacaac tggtcaggag tttgcagaag
    31081 cgttttctcc atctccttga cctgatcagc caaacggata aagtctgcgt ctgctatagg
    31141 ttgcccatta atgcaaattc ggtcattgat ggatacgata tgaggggagg taaaggtcgc
    31201 aacttttttg ccatgcccca taaataattc cctcataaaa gcaatggtgg atcctttccc
    31261 attagtccct gttacgtgga taatagggta agactgctca ggatttccta acaaatccac
    31321 cgcttgctgt attcggccca aacctgatcg aaaattcaaa ccaatccgac tatgaagcca
    31381 ttcttctact tcaaacatac acatctcctt gacaaaagtc caatcaatta tcgcattaaa
    31441 gtatagttac taataaaaac aaggccagga ttttcgtccc gacctcttac ctggttagct
    31501 aataactagc tactatgaat gtgaacgtgg gctaaaaaca tccactgaat gttccaactc
    31561 ttctcaattt ctaggagttg gggtaaaaat gttcactgga cgttccaact cttccccatt
    31621 tctgggagtt gggctgatac agtctcccag actacctgcg tttctaattt ctagcgcagg
    31681 gctaaaaacg tcccccggac gtttttactc ctccataaag ctgttgaaga cttcttcaat
    31741 catgttccat tcgtcttctg agtcttctgg gattggttgc aattcgcctt ctgttccatc
    31801 ttcgttttcg atgaatgagt aagcttggat ttcaacttgt ccgtcttcgt cttcttctgc
    31861 gttaactggt actagaagaa catagttttt accaaattct tcttttccat caattgtcaa
    31921 aaggatttca aacaaggttt catttccttg ctcatctact agtgtgatta gttcacgttc
    31981 ttcgtggtcg tggttatgat cgtgtgacat agcctctcct ttatattaaa attttctatc
    32041 taaataattt tgtaaaatca gctgagctgc taacttatca atgactttct tgcgcttatt
    32101 gcgactgata tctgcttgtt caatcaacat gcgctcagca gccactgttg tcaagcgttc
    32161 atcctgatag tctactggta aaccaaaaaa ctcttctagc tttgctccgt aggcttgact
    32221 agcttctacg cgcggtccac ttgtattgtt catgttttta ggcaagccca ctacaaatcg
    32281 ttccaccttg taagtatcaa ccaattcctt aacgcggtca aaaccaaatt ggccttgttc
    32341 ttcatttatc tggatgattt caagcccttg agctgtaaaa ccaagcggat cgctaatcgc
    32401 cacccctacc gtttttgaac cgacgtccaa tcccataatt ctcataggtt atagatcgac
    32461 tccttgtcct ttgaggtagt agcgaaccaa ttcctcaacg atttcatcac gctcatactt
    32521 acggatttga tttcgtgcat tattataacg aggaacgtag gcagggtctc cactcaatac
    32581 gtaacctacg atttggttaa ttgggttgta acccttatcg ttcaacgaag cataaacgtc
    32641 tgtcaaagtt tcgctaattt cttttttatt ggaatcgtcc aatttaaaac gtactgtttc
    32701 ttcagtaaat cccattctaa caccctcttt ccttagaata gtaccattat agcataattc
    32761 cttaccttct acaattcagg cagtctattt atttggattt tctactgttc tgtcgcacca
    32821 tttgccaatc tatctgaaat atatttgctt ggttcatttt tcaaaagatt ttccaaacca
    32881 atattcttca gatgttccaa ctgggaagcc ttcttgacat ccagaacttg aaaatcaaaa
    32941 ctagtcgttg tttgaagttc cgttgcgctc aatagttttg tttcaagttt gaaacctacc
    33001 aatttacgag cttcaatgat agacttatcc ttctcctccg cttcaagaag agctttttga
    33061 gtttcttcca ctccctgttg gtcaatataa gtcttcaact catcagaaac tggacgtttt
    33121 tgttgaatcg ttaagctcaa aaaagtcttg tccttgtatg taatggtttg agtttgctgg
    33181 ctaccatcat ctgacttggg caagaccaaa gtcttggtta caactgtatt cttctcggca
    33241 ttttcaataa ctggcaatgc cgactgaagc gtatcctttt ctgtttttgt agctggtcca
    33301 gtttcttttt tctgtccgca accaaccagg acaaaaagga aagctagact aacaagaact
    33361 atttttttca tttctttctt ctttcttttt gaaattaaaa tagaataaga ctgggaattg
    33421 ctcccagcct tgatgtttat agagctgcac gtaaacgtgc ttctgcattt tctacattac
    33481 ggacagagcg tggtaggaag gcacgaatat cgtcttcctt gtagccaact tgcaggcgtt
    33541 tttcatctac aaggattggg ctctttaaaa ttctcggtgt ttccataatc agattgagaa
    33601 cttcattgac actcaaatct tcaatatcca ctccaagggc tttggcatag cgatttttag
    33661 acgaaacgat gctggctatt ccgttatctg ttttggttag aatatccagt aattcttctc
    33721 tcgtaattcc ttctttacca aggttttgtt ctttataact taactggtgg gcattgagcc
    33781 aggtttttgc ttttttacag ctagtacaac ttgagactgt ataaatttta atcatgtacc
    33841 tacccctttc gctacatgtt actatcagtt tagtctatta taccataaaa aacatccgac
    33901 ttgcgaccta tttttaa
//
LOCUS       DAVKQL010000021        33583 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVKQL010000021 DAVKQL010000000
VERSION     DAVKQL010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33583
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.021"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            296..2797
                     /locus_tag="VW561_000908"
     CDS             296..2797
                     /locus_tag="VW561_000908"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEU9943190.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWPESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSTEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEIPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVAEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDETSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDTSIAWSEEGLEGSRKFLDRVYRLITSKEIVAENNGALD
                     KVYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEEKLYVDYAKGFIQMIAPFAPHL
                     AEELWQTVAATGDSISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
     gene            2858..3919
                     /locus_tag="VW561_000909"
     CDS             2858..3919
                     /locus_tag="VW561_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943191.1"
                     /translation="MRTQSHKEIVEEYSASRGSYEDCLNYVENTVKNIIKSQSINVHE
                     IIGRVKTVESLEGKVKRKNYSNLAEITDLCGIRIITYFSDDVDKIAELISQEFEVDVE
                     NTIDKRKSEDPTKFGYVSLHYVVGLKEENTSPTLYGRFKNIKLEIQIRTVMQHAWAEI
                     EHDLGYKSKEDIPDQYRRQFARLAGLIELADDDFVQLKNNINNYEQEIKKKLPTSKKE
                     LPIDSSTLMTYVKEDQNYIELLNTIKSSDMDIDFNIDSSFLSTMIQRAKKLDLRTIGD
                     IAELFEKYKDIFLKVLRIEEDIEPCMSISALTPLLHITNVLESVNNINISDEEFMKVS
                     DDEFSDVFGISYPIDVYEG"
     gene            4146..4841
                     /locus_tag="VW561_000910"
     CDS             4146..4841
                     /locus_tag="VW561_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9943192.1"
                     /translation="MPVNEYGQMIGESMEGYTPGELPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQEPVADMEELVSLLNQMLARKDRFYYAIIDRSTGKVLG
                     TFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAF
                     NLPSRRAAERLGFIYEGTFRQAVVYKGRTRDTDWLSMTDKDWPKVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            4853..5269
                     /locus_tag="VW561_000911"
     CDS             4853..5269
                     /locus_tag="VW561_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9943193.1"
                     /translation="MITIKKQEFVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLAI
                     YVALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSALMKEALEDYKDAYQVQ
                     LVTEQTERTLGFYCSMGFEILSTYDCIGMTWINREK"
     gene            complement(6121..6213)
                     /locus_tag="VW561_000912"
     CDS             complement(6121..6213)
                     /locus_tag="VW561_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEU9943194.1"
                     /translation="MMELILKTIIGPIVVGVILRLVDKWLNKDR"
     gene            complement(6554..6646)
                     /locus_tag="VW561_000913"
     CDS             complement(6554..6646)
                     /locus_tag="VW561_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEU9943195.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            6807..7778
                     /gene="ruvB"
                     /locus_tag="VW561_000914"
     CDS             6807..7778
                     /gene="ruvB"
                     /locus_tag="VW561_000914"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU9943196.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            7759..8331
                     /locus_tag="VW561_000915"
     CDS             7759..8331
                     /locus_tag="VW561_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU9943197.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTTVGLRLNEESDFELYAPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            8562..9320
                     /locus_tag="VW561_000916"
     CDS             8562..9320
                     /locus_tag="VW561_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEU9943198.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            9329..10132
                     /locus_tag="VW561_000917"
     CDS             9329..10132
                     /locus_tag="VW561_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU9943199.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKVLLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            10154..11413
                     /gene="rseP"
                     /locus_tag="VW561_000918"
     CDS             10154..11413
                     /gene="rseP"
                     /locus_tag="VW561_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEU9943200.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            11426..13279
                     /locus_tag="VW561_000919"
     CDS             11426..13279
                     /locus_tag="VW561_000919"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEU9943201.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTSEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            13379..14758
                     /gene="bglA"
                     /locus_tag="VW561_000920"
     CDS             13379..14758
                     /gene="bglA"
                     /locus_tag="VW561_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEU9943202.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            14950..16758
                     /gene="glmS"
                     /locus_tag="VW561_000921"
     CDS             14950..16758
                     /gene="glmS"
                     /locus_tag="VW561_000921"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU9943203.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEKYLAGHHFKGQTDTEIAVHLIGKFAEEDGLSVLEAFKRALHIIRGSYAFAL
                     IDSENPDVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDEAGQ
                     VVVDPDIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(16835..16972)
                     /locus_tag="VW561_000922"
     CDS             complement(16835..16972)
                     /locus_tag="VW561_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943204.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEVVDRTDEISNHTYGKATLTWFEEI
                     F"
     gene            17029..18078
                     /locus_tag="VW561_000923"
     CDS             17029..18078
                     /locus_tag="VW561_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU9943205.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLTAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRAEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(18178..21948)
                     /gene="spuA"
                     /locus_tag="VW561_000924"
     CDS             complement(18178..21948)
                     /gene="spuA"
                     /locus_tag="VW561_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEU9943206.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQDPTPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQASSVKEAVQNESV
                     ENSSKKNIPATPDRQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNKKEN"
     gene            22365..22778
                     /gene="rpsL"
                     /locus_tag="VW561_000925"
     CDS             22365..22778
                     /gene="rpsL"
                     /locus_tag="VW561_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEU9943207.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            22798..23268
                     /gene="rpsG"
                     /locus_tag="VW561_000926"
     CDS             22798..23268
                     /gene="rpsG"
                     /locus_tag="VW561_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEU9943208.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            23693..25774
                     /gene="fusA"
                     /locus_tag="VW561_000927"
     CDS             23693..25774
                     /gene="fusA"
                     /locus_tag="VW561_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU9943209.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEEIRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            25878..30269
                     /locus_tag="VW561_000928"
     CDS             25878..30269
                     /locus_tag="VW561_000928"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEU9943210.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVSRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYCSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            30371..30634
                     /locus_tag="VW561_000929"
     CDS             30371..30634
                     /locus_tag="VW561_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEU9943211.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQVLTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            30627..30905
                     /locus_tag="VW561_000930"
     CDS             30627..30905
                     /locus_tag="VW561_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEU9943212.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            30926..31078
                     /locus_tag="VW561_000931"
     CDS             30926..31078
                     /locus_tag="VW561_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943213.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            31100..32341
                     /locus_tag="VW561_000932"
     CDS             31100..32341
                     /locus_tag="VW561_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEU9943214.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKANMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            32351..32581
                     /locus_tag="VW561_000933"
     CDS             32351..32581
                     /locus_tag="VW561_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU9943215.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            32849..33571
                     /locus_tag="VW561_000934"
     CDS             32849..33571
                     /locus_tag="VW561_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU9943216.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
BASE COUNT         9657 a         6275 c         7852 g         9799 t
ORIGIN      
        1 actatacgtt cttcataaat caaaaagcga cataggttgt cggctattta ttgtgaatac
       61 attaattagc attccagttt tatcttcggt ctaaaataag tattttgtgc tatacgagat
      121 aagtttcttg acttactcct tgatttactg cataacaatg ggataaaaag tgggagatag
      181 agcaattcat agtcatcaaa attaatgaga tacagtatac agttttttct ttaaacacat
      241 ttcaaattcc ctcaaaaatg gtataatagt aacatcacaa aattggagag agaccatgag
      301 tttttacaat cataaagaaa ttgagcctaa gtggcagggc tactgggcag aacatcatac
      361 ctttaagaca ggaacagatg catcaaaacc gaagttttat gctcttgata tgttccctta
      421 tccgtctgga gcgggtctgc acgtaggaca cccagaaggt tataccgcaa ccgatatcct
      481 cagccgttac aaacgtgcgc aaggctacaa tgttcttcac ccaatggggt gggatgcttt
      541 tggtttgcct gcagagcaat acgctatgga tacaggtaat gacccagcag aatttacagc
      601 ggaaaatatt gccaacttca aacgccaaat caatgcgctt ggattttctt atgactggga
      661 tcgtgaagtc aacacaacag atccaaacta ctacaagtgg actcagtgga ttttcactaa
      721 gctttacgaa aaaggcttgg cctatgaagc ggaagtgcca gtaaactggg tggaagagtt
      781 gggaacagcc attgccaatg aagaagtgct tcctgatgga acttctgagc gtggaggcta
      841 tccagttgtc cgtaagccga tgcgtcaatg gatgcttaaa attacggcct atgcggagcg
      901 cttgctcaat gacttagacg agcttgactg gccagagtct atcaaggaca tgcaacgcaa
      961 ctggattggt aaatcaactg gtgctaatgt aacttttaaa gttaagggaa cagacaagga
     1021 attcaccgtc tttaccactc gtcctgatac tcttttcggt gcgactttca ccgttttggc
     1081 tcctgagcat gaactagtag atgcaatcac aagcacagag caagcagaag cagtagcaga
     1141 ctacaaacac caagccagtc tgaaatcaga cttggctcgt acagaccttg ccaaagaaaa
     1201 aacaggggtt tggactggtg cttatgccat caatcctgtc aatggtaagg aaattccaat
     1261 ctggattgcg gattacgttc ttgctagcta tggtacaggt gccgttatgg ctgtacctgc
     1321 ccatgaccaa cgtgactggg aatttgccaa acaatttgac cttccaatcg tagaagtact
     1381 tgaaggtgga aatgtagcag aagctgccta cacagaggat ggtcttcatg tcaattcaga
     1441 cttcctagat ggactcaaca aagaagacgc tattgccaag attgtggctt ggttggaaga
     1501 aaaaggctgt ggacaagaga aggttaccta ccgtctccgc gactggctat ttagccgtca
     1561 acgttactgg ggtgagccaa ttcctatcat tcattgggaa gatgaaactt caacagctgt
     1621 tcctgaaact gaattgccac ttgttttgcc tgtaaccaag gacatccgtc cctcaggtac
     1681 tggtgaaagt ccactagcta acttgacaga ctggcttgaa gtgactcgtg aagatggtgt
     1741 caaaggtcgt cgtgaaacca acactatgcc acaatgggca ggttcaagct ggtactacct
     1801 ccgctatatt gatccacaca atactgagaa attggctgat gaggatctcc ttaaacaatg
     1861 gttgccagtg gatatctacg tgggtggagc agagcatgcc gttcttcact tgctttacgc
     1921 tcgtttctgg cacaaattcc tctatgacct cggtgttgtt ccgactaaag aacctttcca
     1981 aaaactcttt aaccaaggaa tgattttggg aaccagctac cgtgaccacc gtggtgctct
     2041 tgtggcaact gataaggttg aaaaacgtga tggttctttc ttccatgtgg aaacaggaga
     2101 agaattggaa caagcgccag ccaagatgtc taaatccctc aagaacgtgg ttaacccaga
     2161 cgatgtggtg gaacaatacg gtgctgatac ccttcgtgtc tatgaaatgt tcatgggacc
     2221 acttgatact tcgattgctt ggtcagaaga aggtctggaa ggaagccgta aattccttga
     2281 ccgtgtttac cgtttgatta caagcaagga aattgttgca gaaaacaatg gcgctctcga
     2341 caaggtttat aatgaaacgg ttaaagctgt tactgagcaa atcgaatccc tcaaattcaa
     2401 cactgccatt gcccaactta tggtctttgt caacgcggct aacaaggaag aaaaacttta
     2461 tgtagactac gctaaaggct ttatccaaat gatcgcccca tttgcacctc acttggcaga
     2521 agaactctgg caaactgttg cagcaacagg tgactcaatc tcttacgtgg cttggccaac
     2581 atgggacgaa agtaaattgg ttgaagacga aatcgaaatc gttgtccaaa tcaaaggaaa
     2641 agttcgtgcc aaactcatgg tagctaaaga cctatcacgc gaagaattac aagaaattgc
     2701 tctcgctgat gaaaaagtca aagcggaaat tgacggtaag gaaatcgtga aagtgattag
     2761 tgtaccaaat aaactcgtta atatcgttgt gaaataattg taaattatag ttaattaaaa
     2821 agagagacag tttttgttca ctttttggga ggtattaatg aggactcaaa gtcataagga
     2881 gattgttgaa gaatattctg cttcacgggg gagttatgaa gattgcttaa attatgtaga
     2941 gaatactgta aagaatatta ttaaaagtca atctattaac gttcatgaaa taattgggag
     3001 agtaaaaact gtagagtctc ttgaaggcaa agtaaaaagg aaaaattatt ctaatttagc
     3061 tgagattacc gatttatgcg gtattcgtat catcacgtac tttagtgatg atgttgataa
     3121 aatagctgag ttaatcagtc aagaattcga ggttgatgta gagaatacta ttgataaacg
     3181 taaaagtgag gatccaacaa aatttggtta tgtttcactt cattatgtgg tgggtttaaa
     3241 agaagagaac acttcaccta ctttatatgg tagatttaag aatataaaat tagagataca
     3301 aattaggaca gttatgcaac atgcctgggc tgaaattgaa catgatttgg gatataaaag
     3361 taaggaagat atacctgatc aatacaggag acaatttgcc agattggcgg gactaattga
     3421 attggctgat gatgattttg ttcagttgaa aaataatatt aataactatg agcaggagat
     3481 aaagaagaaa ttacctactt cgaaaaaaga attaccgata gatagcagca cgcttatgac
     3541 ttatgtaaaa gaagatcaaa attacattga actattgaat acgattaaat catcagatat
     3601 ggatatagat tttaatattg atagttcttt tctatctact atgattcaga gggcgaaaaa
     3661 attggatttg aggactatag gagatatagc tgaattattt gaaaaatata aggatatctt
     3721 tctaaaggta ttgagaattg aggaggatat tgaaccttgt atgagtatat cggcattgac
     3781 tccattattg catatcacta atgtgttaga atcagttaat aatattaata tttctgatga
     3841 agaatttatg aaagtttctg atgatgaatt ttcagatgta tttggtatat cgtacccaat
     3901 tgatgtttat gaaggttaaa aagtaatttt ttaaatatga aaagaggggt ttcctatttg
     3961 actaagttga ataattcgta gtgttgctct ttctaaagtg aaggtaacta aactcgtcaa
     4021 tatcgttgtt agataagaaa taaatccttc agagtagaat ctggaggatt ttttgaatct
     4081 tcttatgaaa gtatgatata ctatgggcaa ctataaagtt tgaaaagtga aacaaggaga
     4141 ataagatgcc agtaaatgaa tatggtcaga tgattgggga gtcaatggaa ggttatacac
     4201 caggtgaact gccttctatt gatttcttag aaggacgtta tgctcggata gaggctctct
     4261 cggtagaaaa acatgcggag gatttgttag ctgtttatgg cccggatact cctcgggaga
     4321 tgtggaccta cctctttcag gaaccagtag cagacatgga ggaactggtc agccttttaa
     4381 atcagatgtt ggctcgtaag gaccgttttt actatgcaat catagacagg tcaactggta
     4441 aggttttggg aactttttct ctcatgcgca ttgaccagaa taaccgagta atagaagtgg
     4501 gagctgtcac tttttctcca gaactcaggg ggacacggat agggacagaa gcccagtatc
     4561 tactggctcg ctatgtcttt gaagagctta actatcgtcg ctatgagtgg aaatgcgatg
     4621 cttttaatct gccatcaaga cgagctgcag aacgtttggg ctttatttat gaaggaacct
     4681 tccgtcaggc agtggtttat aaggggcgta cgagggatac ggattggttg tctatgactg
     4741 ataaggactg gcccaaagtc aaagatcgtt tggaaacatg gttgcgtcct gaaaattttg
     4801 ataaaaatgg acagcagtac aagagcttga gagagcttta agaggtgttg agatgattac
     4861 tattaaaaag caagaatttg tcaagctaga ggatgttttg catctctatc aggctgtcgg
     4921 ttggacaaat tatacccatc aaccagagat gctggagcag gccttatctc attcattagc
     4981 gatttatgtg gcgcttgatg gcgatgctgt ggtgggcttg attcgtttgg ttggagatgg
     5041 attctcatca gtattggttc aggatttaat cgttttacca atctatcaac gtcaagggat
     5101 tggtagtgcc ctaatgaaag aggctttaga ggattacaaa gatgcctatc aagtccagct
     5161 ggtaacagaa cagacagaaa gaaccttggg cttctattgt tctatgggct ttgaaatctt
     5221 atccacctat gattgtatag gaatgacttg gataaacaga gaaaaataat aaaacttggt
     5281 tgttcttaag caaagtttaa ggctggtcta gtatcatata gtcattaaat aaagacctcc
     5341 taactttatt taatgaaatc ctaaaacttt ttttcatcat aatctcctaa tgaagtcacc
     5401 taatcaggtg gcttttttgt agggtgaggt gatgctgata gaaatttttt taaaaatggt
     5461 ataatttgag aaaagttaag ctagttttaa gcttcgtctt gtatcatata gttattaaat
     5521 aaagacctcc taactttatt taataaaatc ctaaactttt ctttttcata ataatctccc
     5581 ttaactccac ccaatcaggt ggagtttttt ggctctattt caggcttttg gggactattc
     5641 taaaaataat ttttcgatat ttttcggtat ttttcggatt ttggtcgggg aattggcggg
     5701 gactttttga gattttggcg gggatttttt tagcgaatat gactaagaaa taggtcggtt
     5761 gtcgcttcag caagttcgtc ctcaacttga ttgtaagttg ctgttaattg aaggactgga
     5821 actgctgtaa taatgatttt gcttgtccag tagtggcgta ttaaatgagg agtgacatat
     5881 aagattgtct cttcgtttac taaatcaaaa tttctatgaa attgatagaa tccctaactt
     5941 tctcttaaaa ttgaaagtct tgttgtaaag cggttaaagc ttgagaagct gaggatgtaa
     6001 agttttgaca aattttgtga actgtgggat aatggagagg aattaaggat tagttctata
     6061 tcatggacta gaaaagaccc caagctaact tccacattaa gcttggggtc ttttttgaca
     6121 ctatctatct ttgtttagcc atttatcgac taagcgaagg atgacaccga ccacaatcgg
     6181 tccgatgata gttttaagga ttagttccat catgggctat ctcacctcct ttcgcaagcg
     6241 gtgtagcagt gccgtagaat attataccac ataagtgcta aactcgggag tcacccaacc
     6301 aggtggagtt ttttgctcta tttcaggctt ttggggacta ttttaaacat cattttccga
     6361 taactttcac ttaatcttat aaactttatt ggacagattc taaagttgga gaagctgaat
     6421 ggatgaaaat ataaaacttt gacaaaatgt gtgaactatg ggataataaa aaggaattga
     6481 gtactagttc tatatcatag gctagaaaag accccaagct cacgaccaaa taagcttggg
     6541 gtcttttttg acactatttg tccttgttta gccatttatc gactatacga agaacgacac
     6601 cgaccacaat tggtccgatg atagttttga gtactaattc catcatgggc tatctcacct
     6661 cctttcgtag gcggtgtagc agtgtcgtag aatattatac cacataagtg ctaaacttag
     6721 gagtcaccca atcaggtggc ttttttgttt gtggcttggt tttttgatat aatagagcca
     6781 tgagtagaat tttagataat gagataatgg gggatgagga gttagtagaa cgcacgctcc
     6841 gtcctcagta tttacgtgaa tatatcggac aggataaggt caaggaccag ctacaaatct
     6901 ttattgaagc tgccaaaatg cgggatgaag cgctggatca tgtgctctta tttgggcctc
     6961 caggtctcgg gaaaacgacc atggcctttg ttattgccaa cgaactggga gtcaatctta
     7021 agcagacgtc gggtccagtc attgaaaaag ccggagatct ggtagctatt ttgaatgagt
     7081 tagagcctgg ggatgtactt tttattgatg agatccatcg tttgccaatg tcagtggaag
     7141 aggtgcttta tagtgctatg gaggacttct acatagatat tatgattggg gctggtgagg
     7201 gtagtcgtag tgttcatttg gagttaccac cttttacctt gattggtgcg acgactcggg
     7261 ctggtatgct ctccaatccg ctacgggcac gttttgggat tacaggccat atggagtatt
     7321 atgcccatgc tgacttgaca gaaattgtcg agcggacggc agatattttt gagatggaaa
     7381 tcactcatga ggcggcatct gagttggccc tacgtagtcg tgggacccct cgtattgcca
     7441 atcgtctcct caagcgcgtg cgcgattttg cccagataat ggggaatggg gtaattgatg
     7501 atattattac cgataaggct ttgactatgc tggatgttga ccatgaaggt ttggactatg
     7561 tggatcaaaa aatccttcgt accatgattg agatgtacag tggaggacct gttggtctag
     7621 gaactctttc tgtgaatatc gccgaagagc gtgagacagt tgaagatatg tatgagcctt
     7681 acttgattca aaaaggtttt atcatgcgga cacggtctgg acgggtggcg actgctaagg
     7741 catatgagca cttaggttat gaatacagtg aaaaataagc aagaaatctt agaggctttt
     7801 agagaaaatc cagatatgat ggccattctg acgatcatcc gagaccttgg tctgaaagac
     7861 tcgtggttgg cagcaggttc tgtcagaaat ttcatttgga atcttttgtc agacaaatcc
     7921 ccttttgatc atgaaacaga tatagatgtg attttctttg atccagattt ttcttatgag
     7981 gaaaccttat tactggagaa aaagctgcga gaggattttc ctcagtacca gtgggaattg
     8041 aaaaatcagg tctatatgca tcagcacagt cctcacactg cttcctatac cagttctcgt
     8101 gatgctatga gtaagtatcc agaacggtgt acgacagttg gactgcgctt gaatgaagaa
     8161 tcagattttg aactctatgc accttatggt ttggaggata ttttgaattt tcaagttcgt
     8221 ccaactcctc atttcttaga aaatgaagac cgaatggaac tctatcaaac acgtttatcc
     8281 aagaaaaatt ggcaggagaa atggaaaaat ttgattttta aaaatactta aggaaacttt
     8341 aagctaggga gtgtacacta agttcataag ttaagaagac cttaacttaa actcctaaaa
     8401 ctttttcata ataatctccc tataaaaata aagtcgccca atcaggcggc ttattttttt
     8461 gaaaaatggg cttggtgcct gagaataaat agcttagtga tagaagaaaa tgggtaaata
     8521 tggtataata aaacgataga tttttgaata ggaataagat catgtttgga ttttttaaga
     8581 aagataaggc tgtggaagta gaggttccga cacaggttcc tgctcatatc ggcatcatca
     8641 tggatggcaa tggccgttgg gctaaaaaac gtatgcaacc gcgagttttt ggacataagg
     8701 cgggcatgga agcattgcaa accgtgacca aggcagccaa caaactgggc gtcaaggtta
     8761 ttacggtcta tgctttttct acggaaaact ggacccgtcc agatcaggaa gtcaagttta
     8821 tcatgaactt gccagtagag ttttatgata attatgtccc ggaactacat gcgaataatg
     8881 ttaagattca aatgattggg gagacagacc gcctgcctaa gcaaaccttt gaagctttaa
     8941 ccaaggctga ggaattgact aagaacaaca caggattgat tcttaatttt gctcttaact
     9001 atggtggacg tgctgagatt acacaggcgc ttaagttgat ttcccaggat gttttagatg
     9061 ccaaaatcaa tccaggtgac atcacagagg aattgattgg taactatctc tttacccagc
     9121 atttgcctaa ggacttacga gacccagact tgattatccg tactagtgga gaattgcgtt
     9181 tgagcaattt ccttccatgg cagggagcct atagtgagct ttattttacg gacaccttat
     9241 ggcctgattt tgacgaagcg gccttgcagg aagctattct tgcctataat cgtcgtcatc
     9301 gccgatttgg aggagtttag gaggaaatat gacccaggat ttacagaaaa gaaccttgtt
     9361 tgcagggatt gccctggcta ttttcctacc aattttaatg attgggggcc tcttgcttca
     9421 gatagcaatc ggaatcatag ccatgctagc catgcatgaa cttttgaaga tgagaggtct
     9481 agagaccatg acgatggagg gcctcttgac cctctttgca acctttgcat tgaccattcc
     9541 cttggagaat tacctgactt ttttgccagt tgatgggaat gtggttgcct atagtgtttt
     9601 gatttcaatc atgttaggaa cgaccgtttt tagcaagtct tatacgattg aggatgcggt
     9661 tttccctctt gctatgagct tctacgtggg ctttggattt aatgctttac tagatgctcg
     9721 tgttgcaggt ttggacaagg ttctcttagc cttgtgtatc gtctgggcga cagacagtgg
     9781 tgcctatctt gttgggatga actatgggaa acgaaagtta gcaccaaggg tatcgcctaa
     9841 taaaaccctt gagggtgcct tgggtggtat tttaggagca attttagtaa ccattatctt
     9901 tatgatagtt gacagtacag ttgctcttcc atatggaatt tacaagatgt cagtctttgc
     9961 tattttcttt agcattgctg gacaatttgg tgatttacta gaaagttcga tcaaacgtca
    10021 ttttggtgtt aaggattctg ggaaatttat ccctggacat ggtggtgttt tggatcgttt
    10081 cgatagtatg ttgcttgtat ttccaatcat gcacttattt ggactctttt aatcaaaaga
    10141 cggaggaaac gctatgctcg gaattttaac ctttattctg gtttttggga ttattgtagt
    10201 ggtgcacgag ttcgggcact tctactttgc caagaaatca gggattttag tacgtgaatt
    10261 tgccatcggt atgggaccta aaatctttgc tcacattggc aaggatggaa cggcctatac
    10321 cattcgaatc ttgcctctgg gtggctatgt ccgcatggcc ggttggggtg atgatacaac
    10381 tgaaatcaag acaggaacgc ctgttagttt gacacttgct gatgatggta aggttaaacg
    10441 catcaatctc tcaggtaaaa aattggatca aacagccctc cctatgcagg tgacccagtt
    10501 tgattttgaa gacaagctct ttatcaaagg attggttctg gaagaagaaa aaacatttgc
    10561 agtggatcat gatgcaacgg ttgtggaagc agatggtact gaggttcgga ttgcaccttt
    10621 agatgttcaa tatcaaaatg cgactatctg gggcaaactg attaccaatt ttgcaggtcc
    10681 tatgaacaat tttatcttag gtgtcgttgt tttttgggtt ttaatcttta tgcagggtgg
    10741 tgtcagagat gttgatacca atcagttcca tatcatgccc caaggtgcct tggccaaggt
    10801 aggagtacca gaaacggcac aaattaccaa gattggctca catgaggtta gcaactggga
    10861 aagcttgatc caagctgtgg aaacagaaac caaagataag acggcaccga ctttggatgt
    10921 gactatttct gaaaagggga gtgacaaaca agtcactgtt acacccgaag atagtcaagg
    10981 tcgttacctt ctaggtgttc aaccgggggt taagtcagat tttctatcca tgtttgtagg
    11041 tggttttaca actgctgctg actcagctct ccgaattctc tcagctctga aaaatctgat
    11101 tttccaaccg gatttgaaca agttgggtgg acctgttgct atctttaagg caagtagtga
    11161 tgctgctaaa aatggaattg agaatatctt gtacttcttg gcaatgattt ccatcaatat
    11221 tgggattttt aatcttattc cgattccagc cttggatggt ggtaagattg tgctcaatat
    11281 cctagaagcc atccgccgca aaccattgaa acaagaaatt gaaacctatg tcaccttggc
    11341 cggagtggtc atcatggttg tcttgatgat tgctgtgact tggaatgaca ttatgcgact
    11401 cttttttaga taatcgagga atattatgaa acaaagtaaa atgcctatcc caacgcttcg
    11461 cgaaatgcca agcgatgctc aagttatcag ccatgctctt atgttgcgtg ctggttatgt
    11521 tcgccaagtt tcagcaggtg tttattctta tctaccactt gccaaccgtg tgattgaaaa
    11581 agctaaaaac atcatgcgcc aagaattcga aaagattggt gctgttgaga tgttggctcc
    11641 tgcccttctt agtgcagaat tgtggcgtga atcaggtcgt tacgaaacct atggtgaaga
    11701 cctttacaaa ctgaaaaacc gtgaaaaatc agactttatc ttaggtccaa ctcacgaaga
    11761 aacctttaca gctattgtcc gtgattctgt taaatcttac aagcaattgc cactcaacct
    11821 ttatcaaatt cagcccaagt atcgtgatga aaaacgcccg cgtaatggac ttcttcgtac
    11881 acgtgagttt atcatgaaag atgcttatag tttccacgct aactatgata gtttggatag
    11941 tgtttatgat gagtacaaag cagcctatga gcgtattttc actcgtagtg gtttagactt
    12001 caaggctatt attggtgacg gtggagccat gggtggtaag gatagccaag aatttatggc
    12061 tattacatct gctcgtacag accttgaccg ctgggttgtc ttggacaagt cagttgcctc
    12121 atttgacgaa attcctgcag aagtgcaaga agaaatcaag gcagaattgc tcaaatggat
    12181 agtctctggt gaagatacca ttgcttactc aagtgagtct agctatgcag ctaacttaga
    12241 aatggcaaca aacgagtaca aaccaagcaa ccgtgttgtc gctgaagaag aagttactcg
    12301 tgttgcaacg ccagatgtta aatcaattga tgaagttgca gctttcctca atgttccaga
    12361 agaacaaacg attaaaaccc tcttctacat tgcagatggt gagcttgttg cagcccttct
    12421 agttggaaat gaccaactca acgaagtcaa gttgaaaaac cacttaggag cagatttctt
    12481 tgacgttgct agcgaagaag aagtggcgaa tgttgttcaa gcaggatttg gttcacttgg
    12541 accagttggt ttgccagaga atattaaaat tattgcagat cgtaaggtgc aagatgttcg
    12601 caatgcagtt gtcggtgcta acgaagatgg ctaccacttg actggtgtga acccaggccg
    12661 tgattttact tcagaatatg tggatatccg tgaagttcgt gagggtgaaa tttccccaga
    12721 tggacaaggt gtccttaatt ttgcgcgtgg tattgagatc ggtcatattt tcaaactcgg
    12781 aactcgctat tcagcaagca tgggagcaga tgtcttggat gaaaatggtc gtgctgtgcc
    12841 aatcatcatg ggatgttacg gtatcggtgt cagccgtctt ctttcagcag tgatggagca
    12901 acacgctcgc ctctttgtta acaaaacgcc aaaaggtgaa taccgttacg cttggggaat
    12961 caatttccct aaagaattgg caccatttga tgtgcatttg attactgtca atgtcaagga
    13021 tgaagaagcg caagccttga cagaaaaact tgaagcaagc ttgatgggag ctggttatga
    13081 agtcttgaca gatgaccgta acgaacgtgt cggtgttaaa tttagcgata gtgacttgat
    13141 tggtcttcca atccgtatca ctgttggtaa aaaagcggcc gatggcattg tagaagttaa
    13201 gatcaaggca actggtgaca ccattgaggt tcatgcagat aacgtgcttg aaacgcttga
    13261 aatcctaagc aagaaataaa aactataatc agaagaaaaa caaggaaaaa atgtaactag
    13321 tttttacctt gttttttctg tataatggga gaaatgagta gataaagagg taaatgttat
    13381 gctaagattt ccaaaggatt ttgtctgggg atcctctact tctggaccgc agacagaagg
    13441 acgtgtagct ggtgacggta agggagacaa tctctgggat tactggttcc aagtggagcc
    13501 aaatcgctac tataatggga ttggttcaga taagacatcg actttttatg aaaattggga
    13561 gcgggatatt gagcttttgt tagagactgg tcacacagtc tttcggactt ctattcagtg
    13621 gtcacggatt tttccacaag gctgtggaaa agtcaaccct caaggtgtgg atttttatcg
    13681 taaggtcttt gaggctatta aggctaaagg aattcgtctg ttagtcaatc tctatcattt
    13741 tgatttacct tttgcccttc aagaggatgg tgatggttgg gaaaataagg cgacagtctc
    13801 agcctatgaa gactatgctc gtttttgttt tgagacttat ggagatttag tggatcaatg
    13861 gattaccttt aacgagccca tcgttcctgt agaatttggt tatttttatg atgcccatta
    13921 tccacataag gtggatgcag aggcagctgt taaggtagcc tatcatacac aattggccag
    13981 cagccgggct gttaaggctt gccatgaact tttgcctgat tccaagattg ggattgtcct
    14041 caacttgaca ccggcttatc cacgtagcca gcatcctgct gatgtcaagg cagctcgtat
    14101 tgcggccctt tttcaggccc aatctttctt agatccatct gtcttgggaa cttatccaca
    14161 ggagttagta gaaatcttgc atgaacacgg tcttttacct gatgctacag aggaagagtt
    14221 ggaactcatt cgtgataata cggtggactt ccttggtgtg aactactatc aacctttgcg
    14281 tgttatggct cctcgatttg ctaagcatcc agagagtcca ctcttaccag aacattttta
    14341 cgagccttat gtgatgcctg gacgtaaaat caattctcac cgtggttggg agatttatga
    14401 gcaagggatt tatgacatcg cccaaaatat caaggaaaat tatggcaata ttgagtggat
    14461 gttgactgag aatggtatgg gtgttgaagg ggaagaaaaa ttccgtcaag atggaatgat
    14521 tcaagatgat taccgtattg actttgtaaa aggtcatctt cgtgaacttc accgtgccat
    14581 tgaagatggt gccaactgta agggctactt gatttggacc tttattgact gctggtcatg
    14641 gctcaacagc tataaaaatc gctatggttt ggtcgaatta gacttggaaa cgcaagaacg
    14701 tcgtctgaag aaatcaggcc actggttcaa agaattaagc gataataatg gattttaaaa
    14761 aggactctga ctgattatcc caatcggtca gagttttttg cttacaggag cttaatttga
    14821 actataccaa tttttactct tgacaagtga aagaaacaag tatatactgt ttttgctgat
    14881 tctataaaag atgaattaga ctataccaat tttaaggaga aagcacagct tgcctgtgtc
    14941 gtatatacta tgtgtggaat tgttggtgtt gttggaaaca caaatgcaac tgatattttg
    15001 attcaagggc ttgaaaagct tgaataccgt ggctatgatt ctgcgggaat ttttgtccta
    15061 gatggtgctg ataaccattt ggtgaaggct gttggtcgta tcgcagaatt gtctgccaag
    15121 acagctggtg ttgagggaac aactggtatc ggacatactc gttgggcaac tcatgggaaa
    15181 ccaacggaag acaatgctca cccacaccga tctgagacag aacgttttgt cttggttcac
    15241 aatggggtaa ttgaaaacta ccttgaaatt aaagaaaaat accttgctgg gcaccacttc
    15301 aaagggcaaa cagatacgga aatcgccgta catttgattg gaaaatttgc ggaagaagac
    15361 ggtctctcag ttcttgaagc ctttaaaaga gctcttcata ttatccgtgg ttcatatgcc
    15421 tttgccttga ttgactctga aaatccagat gtcatctatg tagcgaaaaa caaatctcca
    15481 cttttgattg gtcttgggga aggctacaat atggtctgct cagacgccat ggctatgatt
    15541 cgtgaaacta accaatacat ggaaatccat gaccaagagt tggtaatcgt caaggctgat
    15601 agtgtggaag ttcaagacta tgatggaaat cgtcgtgagc gtgctagcta cactgctgaa
    15661 cttgacttgt cagatatcgg taagggaact tacccttact acatgctcaa ggaaattgac
    15721 gagcaaccaa ctgttatgcg gaaactcatc caagcctaca cagatgaggc tggtcaagtt
    15781 gtcgtagacc cagatatcat taaggctgtt caagacgcag accgcatcta catccttgca
    15841 gctggaacat cttaccatgc aggatttgct tctaagaaaa tgttggaaga attgacagat
    15901 acaccagttg aacttggaat ctcatctgag tggggctacg gtatgccact tctcagcaag
    15961 aaaccactct tcatctttat cagccaatct ggtgaaacag cggatagtcg tcaagttttg
    16021 gtcaaggcta atgaaatggg aattccaagc ttaacagtga caaacgttcc aggttcaacc
    16081 ctctcacgtg aagccaacta taccatgctc cttcacgcag gtcctgaaat tgccgtggca
    16141 tcaactaaag cctatacagc gcaaatcgca gcccttgcct tccttgcaaa agcagtcgga
    16201 gaagcaaatg gtaatgctaa agcgcaagcc tttgacctgg ttcatgaatt gtcaatcgta
    16261 gctcagtcta tcgaatcaac tctttcagag aaagaaacca ttgaagccaa ggttcgtgaa
    16321 cttcttgaaa caactcgtaa cgccttttac atcggacgtg gtcaagatta ctacgtagcc
    16381 atggaagcaa gtctcaaact caaagagatt tcttatatcc agtgtgaagg ttttgcggca
    16441 ggagaactca agcacggaac cattgccttg attgaagaag gaacgcctgt tttggctctc
    16501 ttgtcagatc cagttcttgc caaccatact cgtggaaata tccaagaggt cgcagcccgt
    16561 ggtgccaaag tcctcactat cgcagaagag aatgtagcca aagataccga cgatatcgtc
    16621 cttacgaccg tacatccata cctctcacca atttcaatgg tcgtaccaac gcaattagtc
    16681 gcttactttg caaccctcca ccgtggcctc gatgtggata aaccacgtaa ccttgctaaa
    16741 tcagtaacgg tagaataagc taaaaaagtc tagttatcta ggctttttct aatgagcaaa
    16801 ttggttgctt gtacttaaga ttcgtgttat actcttagaa aatctcttca aaccacgtca
    16861 acgtcgcctt gccgtaggta tggttactga tttcgtcagt tctatccaca acctcaaaac
    16921 actgttttga gcaacctgcg gctagcttcc tagtttgctc tttgattttc attgagtatt
    16981 agaataattt ttcaaattga aggacagaga tagacaagga gaatcacaat ggtagaattg
    17041 ggaatttcaa catttgggga aataacggag cttgaaggga ctggacaaac ttacagtcat
    17101 gctgaacgca ttcgccagtt ggtggcagag attgagctgg ctgacaaggt tggtttggac
    17161 gtatatggga ttggcgagca ccatcgggcg gattttgcag tatcagcccc agagattgtc
    17221 ctgaccgctg gggcagtcaa taccaagaaa atccgtttga ccagtgcagt cagcattctg
    17281 tcaagcatgg acccgattcg tttgttccaa cagtatgcca ctatcgatgc tttgtcaaat
    17341 ggacgagcgg agattatggc tggaaggggc tctttcacgg aatctttccc tctgtttggt
    17401 tatgacttga aagactacga ctctcttttt gatgagaaat tagacttgct tcagttagtc
    17461 aatgaaaaga ccaagttaga ctggcaaggt cgattgactc aaacgattgc tggtaaagaa
    17521 gtttatcctc gtccagttca ggacaaattg cccttgtgga tagctacagg tggtcatgtc
    17581 gaatcaacag tgaagattgc tcaggctggt ctaccgattg tatatgctat tattggtggt
    17641 aatccacgtt attttaaaaa gttgattcag gcttatcgtg agattggaag cgaagcgggc
    17701 catgctgaca aagacttgaa agtgggagcc cattcttggg ggtggattgc ggaagatggt
    17761 gagcaggcgg tgaaagatta tttccatccg actaagcaag tggtggatgc tatttccaaa
    17821 gaccgtccgc actggcagga attgcgttat gagcaatatt tggagcaggt agggccaaat
    17881 ggcgccatgt ttgttggcaa tccagatcag gtggccgaaa aattgattcg catgatagag
    17941 gatttaggat tggaccgctt catgcttcat ctaccgcttg gttctatgcc tcatgaccag
    18001 gttctgagag ctattgaact cttcggaaca caagttgcac ccaaagtacg agcttatttt
    18061 gctatgaaag aggcttaata aaaaatccta atcaagttta ttaggacaac aaatattgta
    18121 cgaaaattaa tcccccgagc tataatgctt gggggatttt tctataggag ggctagttta
    18181 gttctctttt ttatttttta gtaagaaacc gagaccaagg agcgcaagga ggctgattcc
    18241 tgcaaatagg agtttgtttt cgtttttgat tcctgtattt ggaagttcag cttgtctatc
    18301 tggggttgca ggtatatttt tcttgctaga gttttctacc gattcgtttt gaaccgcttc
    18361 ttttacagat gatgcttgtg cttcttgtgc tggttgttca gcttgtgaat cagctgtagc
    18421 ttggctaggt ttattttccg catcagctac tttggcatct ggtttagtag aatcaggtct
    18481 agcttctgga gttgggtctt gatgttcagg cttggaaggg gttgagtctg gtttctcttc
    18541 tgcagttgac tcatggctag ttccattttg agagactcga agaacagtag ctgtaagggc
    18601 attcaatttc aagccttttt cagtccattc aagtcctttc gggttggcaa ttccgactga
    18661 tcctgcttgg ttttcatctg ccaaaacttc cgcatttctt agatgtgcaa aggcagttcc
    18721 caaattaaat tcgcgagctt tttcatccgc attgacaaag actgcgtaga tatcgccgtt
    18781 tggagcagtg atttggtagc caatcactac atcctctttt tccacaccat tttggcctgg
    18841 gacagtgatg aggtggacac ggtctttgat atcttggaga ctcttaagtc ggaaggcatc
    18901 tgtagattga cgaagggcaa tcaaaccttt catatagtca cggctcttga cattttcagg
    18961 ataagcttta ccatctgtag ccttagtcca gtcaaacttg ttgactgcgt cgctagaatc
    19021 gtaagaatca tggatgaagt aaggatagtc aaatgggttg ccgtccttat cacgcaacaa
    19081 gtgagatttg tttggaacct tatcctctgc tactggagtc ttgtaggctg ggtcacggaa
    19141 ttgtttagta cgtccatatt cctgaccgga gtggataaat ggagttcctt gagctgtcaa
    19201 gaccatgaga tttccaagtc gtaaacgacg gtggatttca gcatagttct cagccttgct
    19261 tgggtctttt ttgatagact gggcaatgat gtcaaagagg gtcaagttat catgggctgc
    19321 gatgtattgg atgacatctc cagggctgtc agcttcaaag ttagttggtt gagcaatgag
    19381 atttttaaag atggtgttga catcacgctt gccacctgtg ataaaggcag gttgaccttc
    19441 gtttggataa ccagatttga ggttgttacg gatgtcatct gaaaagacag cgacagtatc
    19501 ggtatgtttc atccaatctt ggtcagcagc tttagtaggc atgttttcat caccggcata
    19561 ggttctccaa ccttcgccta acatgattag atttggattg agggcgcgtg cagccttgta
    19621 agcttcttcg atagaagcgg cgtcatggtc tcccatcata tcgaagcgga agccatccac
    19681 tttgtaggta tcaactaggt atttgataga gtcaactagg agccgtttgg tcatatggtg
    19741 ggttgtcccc aagcgtccac caccaaagct agttcgaggt gtgccatcgg catccataaa
    19801 gtggtagtag tttggctcta ggtcttcaaa gatatcgact ttggctgtgt ggttataaac
    19861 gacatctagg atagctccca taccacgttt gtggatttcg ttgatgaggt ttttaaattc
    19921 tgcgattcgt ttttctggat tcttaggatc gcttgagtac ataccagtca aggagaagta
    19981 gttttgaggg tcatatcccc agttgtagtt gctgttgctt gaagcatagt cagacaagcg
    20041 ttcatggttt ttcaattcat tgacaaagta gtaagacaag actggaagga gctggatatg
    20101 ggttacaccc aagtctttga gatagtctag tttttcaatg aaggcttcaa aagtcccaaa
    20161 tggtttggtc aagtcttttg caatggcagg atctgaagtg aagtcacgca catgagcttc
    20221 gtagataacg gcgtcttcac gagtcttgaa attgtgaatc ttaccataag tcaagtcttg
    20281 aggtccgagt ttagctggat ctacaaaggc ggctttagcc actttatggg catcgtcaat
    20341 cttggcatcg tcgctattcc aagcagcaag agatttagcg taaggatcga gtgcaagaac
    20401 agttttacct tgacgctcga tttggtattg ataatagtag ccagtgaaat ctgtgattcc
    20461 gagtttgttt gtgctgtcta gagtttgttt ccaagttcct ctttcccctt tttcgagagc
    20521 gacagttcca actactttgt cagggtcatt cttgtcgtag acaacaacag aaaccttatc
    20581 agcacttggt gaccaaaggg tcaaatcaac ttgttttcct tcttctttta ggtcagctcc
    20641 cagtttgcca tcatagctgt atgtctcatc tttcaggcgc cagcttgttt tggtagtgaa
    20701 ttggtcggaa ttgtagctaa cagtataagg atgttttgtg tcagagaaat ctccgctgta
    20761 ggtcactttc ttaccagctt catcgattgc aacatcggta atagttacct tgtttcctag
    20821 gtgattagtg atgttggagt gtttgaggat atcttctttt ttagcaccga caagtgttga
    20881 aaagctactt tcaatgctag aagtgcctac gtgttgggct cctgtcatac ggatatcatg
    20941 gacatagtat ggatttgtgt aaatcgattc atcatcgtct tttaggaaaa tttggctatg
    21001 atttttcaaa tctgtgaact tataattttc tttacggatt ttcacgtcgt ctccttgttt
    21061 gctctcatct agtaataaaa atccaaattc tcttgcggct tcattaagag gaatgtcgat
    21121 atagcggcca tatttgcctg tagccgtaaa gtctgttccg tcaggccatt gagcgctact
    21181 tggatttttt acatctcccc agtaccagag agatttcttg tcatagttgc catctgtgcg
    21241 gtagtagttg acgcgaacag ttcctgcagg ctgtggctcg taagagaaaa ccttgtaatc
    21301 ttggtctaac caagcctcgt tcatttttgg aactagtttt tctacagatt tatcgccggt
    21361 tagatttttt ccagctgtat tgttgatgag gaagctaatt ttcttggctt gttctccctt
    21421 taatttgaca tctaggtaat agccgtagtc atctttcttg gcatccttga aggacaaagc
    21481 tccgtttggc cagttttcag atggtttttc aacatcgtcc caagtccata gtccttgagc
    21541 atccttgttt tcttcaggaa gttttttgac atggatacgg aagtagttat cttcgatggg
    21601 ctcttctgcc ttagtttcag ttgttactgg actagtagaa gtagttggct ctgttgcagg
    21661 tttttcttct gttttctcta gggaagcgtt tgcatttcta ggtgaagaaa tatcactttc
    21721 gttcttacca gagccagctg ctgtatcagt aagaggttgg gcaagggtgg ttgtatttct
    21781 gttgttgggt tcgttagtgg ttggtgtact ttcgtcggat gagatagttg gtgtagccat
    21841 ggcaagcaag acaaggcttg cgccgataag gacagaacca gttccatttt tgagggaacg
    21901 gatgctgtag accatttttt tctcagtgtg agatggtgtt tttctcataa tgattctcca
    21961 atcaataaat ttctatatca gtatagcatg tagtaaaaca atatgcaagc gttttctttg
    22021 atttgtgaaa aaaattctta cttttgagaa gttttctgta ctattttata tatcagtata
    22081 aatgacggag ataaaaatgt cacataagcc aagccgtatt attaggaaga tgatgtgttc
    22141 ccagctattt ttattcagaa attgatctct aacattacca ataaaaaaga aaaatatctt
    22201 gacaagcaag ggaaaatttt gttacaataa tagacggtac tttttacttt tggtctctca
    22261 agagtgtaca gggacgtgct gacaaatgtt gcaaaagtac acacagatga tatctgtcac
    22321 caagtgtatc atcaccaaaa ataaaaaaac acaggagaat gtagatgcct acaattaacc
    22381 aattggttcg caaaccgcgt aaatcaaaag tagaaaaatc taaatcacca gctttgaacg
    22441 ttggttacaa tagtcataaa aaagttcaaa caaacgtttc ttcaccacaa aaacgtggtg
    22501 ttgcaactcg tgttggaaca atgacaccta aaaaacctaa ctcagccctt cgtaaattcg
    22561 ctcgtgtacg tttgagcaac cttatcgaag ttactgccta catcccaggt atcggacaca
    22621 acttgcaaga gcacagcgtg gtgcttcttc gcggtggacg tgtaaaagac cttccagggg
    22681 tacgttacca tatcgtccgt ggtgcacttg atactgcagg tgttaacgat cgtaaacaag
    22741 gccgttctaa atacggtact aaacgtccaa aagcataagg aaaggggata aagagaaatg
    22801 agtcgtaaaa atagagctcc aaaacgtgac gtattgccag atccgctata caattcacaa
    22861 ctagttactc gtcttatcaa ccgcgttatg cttgatggta aacgtggtac tgctgcttca
    22921 atcgtttacg gtgcttttga gcaaatcaaa gaagctactg gcaacgatgc acttgaagta
    22981 tttgaaacag ctatggaaaa catcatgcct gtacttgaag tacgtgcacg tcgtgttggt
    23041 ggttctaact accaagtccc agttgaagtt cgtccagaac gtcgtacaac acttggactt
    23101 cgttggttgg taacaatcgc tcgtcttcgt ggtgaacaca caatgcaaga ccgtcttgca
    23161 aaagaaatct tggatgctgc taacaacact ggtgcagctg ttaagaaacg tgaagatact
    23221 caccgtatgg ctgaagctaa ccgtgcattc gcacacttcc gttggtaaga taggatgcga
    23281 aagcgttaag aaagtcccag agaaaatagg gaatcgaagc aggttgcggt tgcaaccaat
    23341 gagattcatc tttttctcca gacttttagc ttgagctcaa ctaaatcatg atgctaggca
    23401 cggtaaggat gcaaggtgaa aataggaaac tgacgcagta ttcaacgaat acaaggagtt
    23461 ttatcttttt cacgcagcat cccgttccag ctcacatcgg ctaactaact ttagcccggg
    23521 ttcaaattag ctaaatcgat tagtattagc tataactcag cttaccatct cgtaagttga
    23581 aaccaacaat agcatgaaaa cattgagaac gggtaggtcc tgcctatccg tttttattaa
    23641 aatcgtgtta taatagaata gaaatcaaaa ataaatagga gaaacaaacc tcatggcacg
    23701 cgaattttca cttgaaaaaa ctcgtaatat cggtatcatg gctcacgtcg atgccggtaa
    23761 aacaacaact actgagcgta ttctttacta cactggtaaa atccacaaaa tcggtgaaac
    23821 tcacgaaggt gcgtcacaaa tggactggat ggagcaagag caagaacgtg gtatcacgat
    23881 cacatctgct gcgacaacag ctcaatggaa caaccaccgc gtaaacatca tcgacacacc
    23941 aggacacgtg gacttcacaa tcgaagtaca acgttctctt cgtgtattgg atggtgcggt
    24001 taccgttctt gactcacaat caggtgttga acctcaaact gaaacagttt ggcgtcaagc
    24061 aactgagtac ggagttccac gtatcgtatt tgccaacaaa atggacaaaa tcggtgctga
    24121 cttcctttac tctgtaagca cacttcacga tcgtcttcaa gcaaatgcac acccaatcca
    24181 attgccaatc ggttctgaag atgacttccg tggtatcatt gacttgatca agatgaaagc
    24241 tgaaatctat actaacgacc ttggtacgga tatccttgaa gaagacatcc cagctgaata
    24301 ccttgaccaa gctcaagaat accgtgaaaa attgattgaa gcagttgctg aaactgacga
    24361 agaattgatg atgaaatacc tcgaaggtga agaaatcact aacgaagaat tgaaagctgg
    24421 tatccgtaaa gcgactatca acgttgaatt cttcccagta ttgtgtggtt cagccttcaa
    24481 aaacaaaggt gttcaattga tgcttgatgc ggttatcgac taccttccaa gtccacttga
    24541 catcccagca atcaaaggta ttaacccaga tacagacgct gaagaaattc gtccagcatc
    24601 tgacgaagag ccatttgcag ctcttgcctt caagatcatg actgacccat tcgtaggtcg
    24661 tttgacattc ttccgtgttt actcaggtgt tcttcaatca ggttcatacg tattgaatac
    24721 ttctaaaggt aaacgtgaac gtatcggacg tatccttcaa atgcacgcta acagccgtca
    24781 agaaatcgac actgtttact caggtgatat cgctgctgcc gttggtttga aagatactac
    24841 aactggtgac tcattgacag atgaaaaagc taaaatcatc cttgagtcaa tcaacgttcc
    24901 agaaccagtt atccaattga tggttgagcc aaaatctaaa gctgaccaag acaagatggg
    24961 tatcgccctt caaaaattgg ctgaagaaga tccaacattc cgcgttgaaa caaacgttga
    25021 aactggtgaa acagttatct caggtatggg tgaacttcac cttgacgtcc ttgttgatcg
    25081 tatgcgtcgt gagttcaaag ttgaagcgaa cgtaggtgct cctcaagtat cttaccgtga
    25141 aacattccgc gcttctactc aagcacgcgg attcttcaaa cgtcagtctg gtggtaaagg
    25201 tcaattcggt gatgtatgga ttgaatttac tccaaacgaa gaaggtaaag gattcgaatt
    25261 cgaaaacgca atcgtcggtg gtgtggttcc tcgtgaattt atcccagcgg ttgaaaaagg
    25321 tttggtagaa tctatggcta acggtgttct tgcaggttac ccaatggttg acgttaaagc
    25381 taagctttat gatggttcat atcacgatgt cgactcatct gaaactgcct tcaagattgc
    25441 ggcttcactt tcccttaaag aagctgctaa atcagcacaa ccagctatcc ttgaaccaat
    25501 gatgcttgta acaatcactg ttccagaaga aaaccttggt gatgttatgg gtcacgtaac
    25561 tgctcgtcgt ggacgtgtag atggtatgga agcacacggt aacagccaaa tcgttcgtgc
    25621 ttacgttcca cttgctgaaa tgttcggtta cgcaacagtt cttcgttctg catctcaagg
    25681 acgtggtaca ttcatgatgg tatttgacca ctacgaagat gtacctaagt cagtacaaga
    25741 agaaattatt aagaaaaata aaggtgaaga ctaatccgtc ctcactctag aaggaagtca
    25801 cttagtggct tccttttgtc tttagaaaat acctctaaat atggtaaaat agtagaagaa
    25861 taatgtgagg aaaatgaatg tcaaatagtt ttgaaatttt gatgaatcaa ttggggatgc
    25921 ctgctgaaat gagacaggct cctgctttag cacaggccaa tattgagcga gttgtggttc
    25981 ataaaattag taaggtatgg gagtttcatt tcgtattttc taatatttta ccgattgaaa
    26041 tctttttaga attaaagaaa ggtttgagcg aagaattttc taagacaggc aataaagctg
    26101 tttttgaaat taaggctcgg tctcaagaat tttcaaatca gctcttgcag tcctactata
    26161 gggaagcttt ctctgaaggt ccatgtgcta gtcaaggttt taagtccctt tatcaaaatt
    26221 tgcaagttcg tgctgagggt aatcagctat ttattgaagg atctgaagcg attgataagg
    26281 aacattttaa gaagaatcat cttcctaatt tagccaaaca acttgaaaag tttggttttc
    26341 caacttttaa ctgtcaagtc gagaagaatg atgtcctgac ccaagagcag gaagaggcct
    26401 ttcatgctga aaatgagcag attgttcaag ctgccaatga ggaagcgctc cgtgctatgg
    26461 aacaactgga gcagatggca cctcctccag cggaagagaa accagtcttt gattttcaag
    26521 cgaaaaaagc tgcagctaaa cccaagctgg ataaggcgga gattactcct atgatcgaag
    26581 tgacgacaga ggaaaatcgt ctggtatttg aaggggttgt ttttgatgtg gagcaaaaag
    26641 tgactagaac aggtcgtgtt ttaatcaact ttaaaatgac ggactatact tcaagttttt
    26701 ctatgcaaaa gtgggttaaa aacgaggaag aggcccagaa gtttgacctc atcaagaaga
    26761 attcttggct ccgagttcga gggaatgtgg agatgaataa cttcacacgc gatttgacta
    26821 tgaacgtaca ggatctgcag gaagttgttc actatgagcg gaaggatttg atgccagaag
    26881 gtgagcgtcg ggttgagttt catgctcata ctaacatgtc gactatggat gctttgccag
    26941 aggtcgaaga gattgttgca acagctgcta agtggggaca caaggcggtt gctatcacag
    27001 accatgggaa tgtccagtcc tttccacatg gctataaggc ggctaagaaa gcgggaatcc
    27061 agctgatcta tgggatggaa gccaatatcg tggaggaccg tgtccctatc gtctataacg
    27121 aagtggagat ggacttatca gaagcaacct acgtggtctt tgacgtggaa acgacgggac
    27181 tttcagctat ctataatgac ttgattcagg ttgcggcctc taagatgtac aaggggaatg
    27241 ttattgctga atttgatgaa tttatcaatc ctgggcatcc cttgtcagct tttactacag
    27301 agttaactgg aattacagat gatcatgtca aaaatgccaa accactagaa caagttttgc
    27361 aagaattcca agaattttgc aaggatacgg tcctagttgc ccacaatgct acctttgacg
    27421 ttggctttat gaatgctaat tatgagcgtc atgatcttcc aaagattagt cagccagtta
    27481 ttgatacgct ggagtttgct agaaacctct atcctgagta taaacgccat ggtttggggc
    27541 ctttgaccaa gcgttttggt gtggccttgg aacatcacca catggccaac tacgatgcgg
    27601 aagcgactgg tcgtctgctt ttcatcttta tcaaagaggt agcagaaaaa catggtgtga
    27661 ccgatttagc tagactcaac attgatctaa tcagtccaga ttcttacaaa aaagctcgga
    27721 tcaagcatgc gaccatctat gtcaagaatc aggtaggtct aaaaaatatc tttaagctgg
    27781 tttccttgtc taataccaag tattttgaag gagtgtcacg gattccgaga acggttctag
    27841 atgcccatcg agagggcttg attttaggtt cagcctgttc agagggtgaa gtttttgacg
    27901 tggtcgtttc tcaaggtgtg gatgcggcgg ttgaggtggc caagtattat gactttatcg
    27961 aggtcatgcc accggctatc tatgcgccct tgattgctaa agagcaggtc aaggatatgg
    28021 aggaactcca gaccattatc aagagtttga tagaggttgg agaccgcctt ggcaagcctg
    28081 ttttggctac gggaaatgtt cactatatcg aaccggaaga agagatttat cgtgaaatta
    28141 tcgtccgtag tttgggacag ggtgcgatga ttaaccgaac tatcggtcat ggtgaacatg
    28201 cccaaccagc accacttcca aaggctcatt ttcgaacgac taatgagatg ttggatgaat
    28261 ttgccttttt gggagaggaa ctggctcgta aactggttat tgaaaacacc aatgccttgg
    28321 cagaaatatt tgaacccgtt gaagtcgtta agggtgactt gtatacgcct ttcatcgaca
    28381 aggctgaaga aacagttgct gagttgacct ataagaaagc ttttgagatt tatggaaatc
    28441 cgctgccaga tattgttgat ttgcggattg aaaaagaatt aacatccata ctggggaatg
    28501 gatttgctgt gatttatctg gcatcgcaga tgctggtgca acgttctaat gaacggggtt
    28561 atttggttgg ttctcgtggg tctgtcggat ctagtttcgt tgcgaccatg attgggatta
    28621 cggaggtcaa tcctctctct cctcactatg tctgtggtca gtgtcagtac agtgagttta
    28681 tcacagatgg ttcgtacggt tcaggatttg atatgcccca taaggactgt ccaaactgtg
    28741 gtcacaaact cagtaaaaac ggacaggata ttccgtttga gaccttcctt ggttttgatg
    28801 gggataaggt tcctgatatt gacttgaact tctcgggaga agatcagcct agcgcccact
    28861 tggatgtgcg tgatatcttt ggtgaagaat atgccttccg tgcgggaaca gttggtacgg
    28921 tagctgccaa gactgcctat ggatttgtca agggttacga gcgagattat ggcaagtttt
    28981 atcgtgatgc agaagtagaa cgcctcgctc aaggagcggc gggtgtcaag cggacaacag
    29041 gccaacaccc ggggggaatc gttgttattc cgaactacat ggatgtctac gattttacgc
    29101 ctgtccagta tccagcagat gatgtcacgg ctgaatggca gaccactcac tttaacttcc
    29161 acgatatcga tgagaacgtc ctcaaactcg atgtactggg acatgatgat ccgaccatga
    29221 ttcgaaaact tcaggatttg tctggtattg accctaataa aattcctatg gatgacgaag
    29281 gcgtgatggc actcttttct gggactgatg tgctaggggt aacacctgaa caaattggaa
    29341 cgcctacggg tatgttgggg attccagagt ttggaacaaa tttcgtacgt ggaatggtag
    29401 acgaaaccca tccgacaacc tttgcggaat tgcttcagct gtctggtctg tcccacggta
    29461 ctgatgtttg gttggggaat gctcaggatc tgattaagca aggaatagcg gacctatcga
    29521 ctgttatcgg ttgtcgggac gacatcatgg tttacctcat gcatgcgggt ctggaaccta
    29581 agatggcctt tactattatg gaacgggtac gtaagggttt gtggctaaag atttcagaag
    29641 aggagagaaa tggctatatc gaagcaatga aggctaataa ggtgccagag tggtatatcg
    29701 aatcctgtgg gaaaattaag tacatgttcc ctaaggccca tgcggcagcc tacgttatga
    29761 tggccttgcg tgtagcttac ttcaaggttc accatcctat ttattactac tgtgcttact
    29821 tctccattcg tgctaaggct tttgatatca agaccatggg tgcgggcttg gaggtcatca
    29881 agcgcagaat ggaagaaatc tctgaaaaac ggaagaacaa tgaagcctct aatgtggaaa
    29941 tcgatctcta tacaactctt gagattgtca atgagatgtg ggaacgaggt ttcaagtttg
    30001 gtaaattaga tctctactgt agtcaggcga cagagttcct catcgacggg gataccctta
    30061 tcccaccatt tgtagcaatg gatggtctgg gagagaacgt tgccaagcaa ctggtgcggg
    30121 cgcgtgaaga gggagaattc ctctctaaaa cagaactacg caagcgtggt ggactctcat
    30181 caaccttggt tgaaaagatg gatgagatgg gtattcttgg aaatatgcca gaggataacc
    30241 agttgagttt gtttgatgag ttgttttaaa aaattgctta ataatctatt aaaagaggct
    30301 aacgtatatc caatagattt acattagctt tcttttttgt taaaatagtc tatggaaaga
    30361 gggtgagagt atgtcaaaga tgagtataag catccgtctg gatagtgagg ttaaggagca
    30421 ggcccaacag gtgtttagta atctgggaat ggatatgaca acagctatta atattttcct
    30481 tcgtcaggca attcaatatc agggattacc ttttgatgtt agactagacg aaaatcggaa
    30541 gttgctccaa gtgttaacgg atttagacca aaatcgtaat atgagccagt cttttgaatc
    30601 agtctcagat ttgatggagg acttacgtgc ttaagattcg ttatcataaa cagtttaaaa
    30661 aagattttaa gttggctatg aagcgtggtt tgaaggcaga attattagaa gaagttttga
    30721 attttctggt tcaagaaaaa gaacatcctg ccagatatcg tgatcattca ttgacggcat
    30781 ccaagcattt tcaaggagtt cgtgaatgcc atacccagcc agattggctt ttggtttata
    30841 aagtagacaa gtcggaattg attttaaatt tgctgaggac aggcagtcac agtgatttat
    30901 tttaatctat tttaaggggg ttctcatgaa actaagaata tttgcggaag ataagccggc
    30961 taagaaggta tttgaatatc aattagaact tgctgatcgt acaattcttc tatcgacagc
    31021 actcttgtca ggtgctattg ctttagcagg aatcttttct gctttgaaag aaaaataaaa
    31081 atagaaaaga gaaaacagaa tggttttacc aaattttaaa gaaaatctag aaaaatatgc
    31141 gaaattgttg gttgcgaacg gaattaacgt gcaacctggt cacactttgg ctctctctat
    31201 tgatgtggag caacgtgaat tggcacatct aatcgtgaaa gaagcttatg ccttgggtgc
    31261 gcatgaggtc atcgttcagt ggacagatga tgtgattaac cgtgagaaat tcctccatgc
    31321 cccgatggag cgtttggaca atgtgccaga atacaagatt gctgagatga actatctctt
    31381 ggagaacaag gctagccgtc ttggagttcg ttcatctgat ccaggtgcct tgaacggagt
    31441 ggacgctgac aagctttcag cttctgctaa agctatggga cttgccatga agcctatgcg
    31501 tatcgcaact caatctaaca aggttagctg gactgtagca gctgcagcag gacttgagtg
    31561 ggctaagaaa gtcttcccaa atgctgcgag cgacgaagaa gcagttgatt tcctttggga
    31621 ccaaattttc aaaacttgcc gtgtctacga agcagatcct gttaaggctt gggaggaaca
    31681 tgcagccatt ctcaagagca aggccaatat gcttaataag gagcaatttt cagcccttca
    31741 ctacacagcg ccaggaacag atttaacact tggtttgcca aagaaccacg tttgggaatc
    31801 agctggtgct gtcaatgcac agggcgaaga attcttgcca aatatgccaa cagaagaggt
    31861 cttcacagcg cctgacttcc gtcgtgcaga tggttatgtc acttctacaa aaccgcttag
    31921 ctacaacgga aatatcattg aaggcattaa ggtgaccttt aaggatggac aaatcgtaga
    31981 tatcactgct gagaagggtg atcaggttat gaaagacctt gtctttgaaa atgcgggtgc
    32041 gcgtgccttg ggtgaatgtg ccttggtacc agatccaagt ccaatttctc agtcaggcat
    32101 taccttcttt aacacccttt tcgatgaaaa tgcgtcaaac cacttggcta tcggtgcagc
    32161 ctatgcgact agcgttgttg atggagcgga gatgagcgaa gaggagcttg aagctgcagg
    32221 gcttaaccgt tcagatgttc acgtagactt tatgattggt tctaaccaaa tggatatcga
    32281 tggtattcgt gaggatggaa cgcgagtacc tcttttccgt aatgggaatt gggcaaatta
    32341 aggagataat atgttaggaa gtatgttcgt tggtctctta gtgggatttt tagcaggtgc
    32401 tatgaccaat cgtggagagc gaatgggatg ttttggaaaa atgtttctcg gttggatcgg
    32461 agcctttcta ggtcacttgc tctttggaac ttgggggcca gttttatcag gaacagctat
    32521 tatcccagcg attttaggag ccatgattgt tttagctatt ttttggagac gaggaagtta
    32581 atttcctaaa tctgatactc aatgaaaatc aaagagcaaa ctaggaaact agccgcaggt
    32641 tgctcaaagc actgctttga ggttgcagat agaactgacg aagtcagcta aaaacactgt
    32701 tttgaggttg cagatagaac tgacgaagtc agtaacatat atacggcaag gcgacgttga
    32761 cgcggtttga agagattttt gaagagtatg aaacgaaaag gaggttggtc attgtactag
    32821 cctccttttg atatgatata atagttctat gagattagat aaatttttag ttgcctgtgc
    32881 ggtgggaagt cggactgagg tcaaaaactt gctcaaggct gggcgcgtga ctgtaaatgg
    32941 taaaaaagaa aaatcagcta aattgcagat tgatgaaaaa atagatgaga ttcgctttga
    33001 tgggcaagtg ttggagtatg aagagtttgt ctactacatg atgaacaagc ccaaaggagt
    33061 tatctcagcg actgaggatc ccaagcacag aaccgttctg gacttgttgg atgacttggc
    33121 gcggagtaag gaagttttcc cagtaggacg cttggatatt gacacgcatg gtcttttgct
    33181 cttgaccaat gatggtcagc tggctcatgt tcttctttcg cccaagcgtc atgtggacaa
    33241 gacttatctg gcacaagtca agggaatcat gacccaagaa gatgtggaga catttgctga
    33301 gggtattcct ctcaaagact ttacctgtca acccgctata ctggagcttg tatccataga
    33361 tacagaaaag aatcaaagcc aaatccgtgt gaccattgca gaagggaagt ttcatcagat
    33421 caagcgtatg gtgggctact gtggcaagga agtggtggac ttgcaacgat tgactatggg
    33481 aactttagta ttagatgaga acctggaacg aggggaatgg cgtcgcttga ccaaggaaga
    33541 attagaaatt ctccgcgcta atattattta atttagttta ata
//
LOCUS       DAVKQL010000022        32878 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVKQL010000022 DAVKQL010000000
VERSION     DAVKQL010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32878
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.022"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(162..929)
                     /locus_tag="VW561_000935"
     CDS             complement(162..929)
                     /locus_tag="VW561_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEU9943217.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDKLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEIGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(926..1792)
                     /locus_tag="VW561_000936"
     CDS             complement(926..1792)
                     /locus_tag="VW561_000936"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEU9943218.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNVGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(1829..3196)
                     /gene="accC"
                     /locus_tag="VW561_000937"
     CDS             complement(1829..3196)
                     /gene="accC"
                     /locus_tag="VW561_000937"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU9943219.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNANFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(3208..3630)
                     /gene="fabZ"
                     /locus_tag="VW561_000938"
     CDS             complement(3208..3630)
                     /gene="fabZ"
                     /locus_tag="VW561_000938"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEU9943220.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            complement(3627..4112)
                     /locus_tag="VW561_000939"
     CDS             complement(3627..4112)
                     /locus_tag="VW561_000939"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEU9943221.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(4115..5350)
                     /gene="fabF"
                     /locus_tag="VW561_000940"
     CDS             complement(4115..5350)
                     /gene="fabF"
                     /locus_tag="VW561_000940"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU9943222.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(5372..6103)
                     /gene="fabG"
                     /locus_tag="VW561_000941"
     CDS             complement(5372..6103)
                     /gene="fabG"
                     /locus_tag="VW561_000941"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEU9943223.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVVAIDGGLSM"
     gene            complement(6137..7057)
                     /gene="fabD"
                     /locus_tag="VW561_000942"
     CDS             complement(6137..7057)
                     /gene="fabD"
                     /locus_tag="VW561_000942"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU9943224.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDRTAHLAHVEDQASLVALLEK"
     gene            complement(7050..8024)
                     /gene="fabK"
                     /locus_tag="VW561_000943"
     CDS             complement(7050..8024)
                     /gene="fabK"
                     /locus_tag="VW561_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEU9943225.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     VSIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVE
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWAGVVRND"
     gene            complement(8143..8367)
                     /locus_tag="VW561_000944"
     CDS             complement(8143..8367)
                     /locus_tag="VW561_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU9943226.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(8427..9401)
                     /locus_tag="VW561_000945"
     CDS             complement(8427..9401)
                     /locus_tag="VW561_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEU9943227.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(9401..9835)
                     /locus_tag="VW561_000946"
     CDS             complement(9401..9835)
                     /locus_tag="VW561_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9943228.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(9929..10714)
                     /locus_tag="VW561_000947"
     CDS             complement(9929..10714)
                     /locus_tag="VW561_000947"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEU9943229.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            11039..12403
                     /locus_tag="VW561_000948"
     CDS             11039..12403
                     /locus_tag="VW561_000948"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEU9943230.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDVTGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            12406..12777
                     /locus_tag="VW561_000949"
     CDS             12406..12777
                     /locus_tag="VW561_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEU9943231.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            12991..14265
                     /gene="serS"
                     /locus_tag="VW561_000950"
     CDS             12991..14265
                     /gene="serS"
                     /locus_tag="VW561_000950"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEU9943232.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILIK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEIKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSATKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(14290..15189)
                     /locus_tag="VW561_000951"
     CDS             complement(14290..15189)
                     /locus_tag="VW561_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEU9943233.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWGLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(15299..15847)
                     /locus_tag="VW561_000952"
     CDS             complement(15299..15847)
                     /locus_tag="VW561_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU9943234.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(16065..17387)
                     /locus_tag="VW561_000953"
     CDS             complement(16065..17387)
                     /locus_tag="VW561_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEU9943235.1"
                     /translation="MLELLKSIDDFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMAIIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(17478..17566)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(17730..20066)
                     /locus_tag="VW561_000954"
     CDS             complement(17730..20066)
                     /locus_tag="VW561_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEU9943236.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRKVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDAFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(20170..20718)
                     /locus_tag="VW561_000955"
     CDS             complement(20170..20718)
                     /locus_tag="VW561_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEU9943237.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQIYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(20715..21017)
                     /gene="zapA"
                     /locus_tag="VW561_000956"
     CDS             complement(20715..21017)
                     /gene="zapA"
                     /locus_tag="VW561_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEU9943238.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            21104..21985
                     /locus_tag="VW561_000957"
     CDS             21104..21985
                     /locus_tag="VW561_000957"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEU9943239.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLSVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLNFC
                     AKLHFKNTEKAKKRLER"
     gene            21989..22603
                     /gene="lepB"
                     /locus_tag="VW561_000958"
     CDS             21989..22603
                     /gene="lepB"
                     /locus_tag="VW561_000958"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU9943240.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            22837..25203
                     /locus_tag="VW561_000959"
     CDS             22837..25203
                     /locus_tag="VW561_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEU9943241.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGEQKSHDLETIQKHITTIEEELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDLRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDECSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSKESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKRDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(25251..26534)
                     /locus_tag="VW561_000960"
     CDS             complement(25251..26534)
                     /locus_tag="VW561_000960"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEU9943242.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDQKFGEEALYQDAMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVITAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEETYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
     gene            complement(27333..28469)
                     /locus_tag="VW561_000961"
     CDS             complement(27333..28469)
                     /locus_tag="VW561_000961"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEU9943243.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKNGFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQKTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQVLDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPAQSHEDSLFVVVEPFNEWVVETKRLKNPDLRLKDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHK
                     VIIERFENPFIVDEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYAFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIEQIVEYI"
     gene            complement(28531..28968)
                     /locus_tag="VW561_000962"
     CDS             complement(28531..28968)
                     /locus_tag="VW561_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9943244.1"
                     /translation="MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKNDVLKSGITVVQVPRGVDFGNVSNPQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            complement(28970..30925)
                     /locus_tag="VW561_000963"
     CDS             complement(28970..30925)
                     /locus_tag="VW561_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9943245.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSMQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEITKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECTKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTRLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQAYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKVLFNFLFHHIRLSLGVPILFQGENLPESV
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIDVIS
                     SFPTVKELFELQERLQYVQAHRTIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRDYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKREEEEVKRCLLMLMSKEASEEARDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            complement(30949..32718)
                     /locus_tag="VW561_000964"
     CDS             complement(30949..32718)
                     /locus_tag="VW561_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEU9943246.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGAIATVGAITGSSVPMFIGA
                     MVMGPLGGWTIKKFDEKFQEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     GAVGNGVEAIVNARLLPMANIIIEPAKVLFLNNALNHGIFTPLGVEQVAQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMVIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIMATAPKGVWPHINVLLGVLVAAVVSFLVA
                     ALILHADKSTEDSLEAAQAATQAAKAQSKGQLVSTSVDTVVLTDSVEKIIFACDAGMG
                     SSAMGASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHV
                     SVDNFLASPRYDEIVASLTGASPIAEIEGDIPTSAPVDSQEIDLNHIDAVVVAYGKAQ
                     GTATMGCETIRAIFRNKNIRIPVSTAKISELGEFNSKNIMIVTTISLQAEVQQAAPNS
                     QFLIVDSLVTTPEYDKMAARMYK"
BASE COUNT         9687 a         7437 c         6232 g         9522 t
ORIGIN      
        1 actgttatag attcaataca ctataaattt ttgtaagctt acatctacag tatacatctt
       61 ttttgtctat tttatagccc atctcctcaa ctgtcaattt ttcaacctga attgcatttt
      121 tccataaaaa atgagacctt tctagtctca tttagtcatt cttagtattt tctaaatcgt
      181 tgatagcgtt cttccagcaa ctcttctagc ggtttttgtg aaagtctagc cagctccgtt
      241 tggagttctt ttttgacact cttaatcagt tctttactag aaagtcctat ttcagaaatc
      301 accttatcca ccacgtccat ttctaacagt tcatgcgaag tgattttcat cagttctgct
      361 gcttccatag cgcgagtacc gtccttccat aaaatggaag caaagccttc tggactgaga
      421 atggcataga tagaattttc cagcatccag acacggtccg cgacagctag agccagagcc
      481 ccgcctgaac caccttcacc gataataatg gcgataatag gaactttcag gtcactcatt
      541 tccatgagat tgcgagcgat agcttcccct tgaccacgtt cttccgctcc gacaccagga
      601 taagcacctg ctgtattgat aaaggtcaca actggacggc caaatttctc agcctgtttc
      661 atcaaccgca gtgcctttcg gtagccttct ggatgtggtt ggccaaaatt ccgtttgagt
      721 ttgtcttgca aactcttgcc tttttggata ccaaccactg ttacagcttg atctccaagc
      781 caaccaatac caccaacaac tgcaccatca tcacgaaaag aacggtcacc atgtaattgg
      841 ataaattcat caaaaatgcc tgtcgcaaag tccaaggttg tcaagcgact ctgctcacgc
      901 gcttctctga ctatttttgc aatattcatc taggactccc tccatgcaat ctgactaggc
      961 tagcaatcgt atctggtaag tctcttcttt tgacaatagc atccacaaag ccatgttcta
     1021 ataggaattc tgccttttgg aaatcctcag gcaagctttc acgaaccgta ttttcaatca
     1081 cacgacgccc agcaaaacca accaagctct gtggttcagc cagaatgata tcgccttcca
     1141 tagcgaaaga agctgtcaca ccaccagtcg ttggatctgt caaaatggtc aggtaaaaga
     1201 gaccaacatt tgaatggcgt ttaaccgccg cagagatctt agccatctgc atgagactca
     1261 tgattccttc ctgcatacgg gctccaccag aggctgtgaa taggacaact ggcaattttt
     1321 cgacagtcgc atactcaaac aaacgagtga ttttttcacc tacaaccgta cccatagaag
     1381 ccatgataaa gttagaatcc ataatcccaa gagccacagt ctgaccttta ataagagcag
     1441 ttcctgtcac aacggcttca tgcagacctg ttttttcacg catagatgcc agtttctttt
     1501 ggtaaccagg gaaatgcaag ggatccttgc tttcaatccc tgtaaacaat tctttgaagg
     1561 ttcccatatc aatcgtcaaa gccaagcgtt cttgggcaga aatacgaaag gtatagctac
     1621 agtgcggaca gatacgttca cttcccagat ccttctgata gatggtatgc ttacagcctg
     1681 gacactggga aaataattca tctggaacct ctggcttagc ttgaggtttt tccctaaccg
     1741 aacgattggg attgattcga atatacttat cttttttact aaatagagcc attgattccc
     1801 cttttcggtt taaactctta aagtcatttt attctttttc ttgatattta ggtaagaagg
     1861 tttccatcaa gaaggaagta tcataatccc cagcaatgac attgcgatct gaaatgaggt
     1921 caagctggaa atttgcattg gtctgcactc cttcaatctc taattcatag agggcacgtt
     1981 gcattttcat caaggcgtca aaacgatttt cgccgtgtac tatgattttg gcaatcatac
     2041 tatcataata aggcggaatg gtataacctg gataaactgc tgaatccacg cgcaagccaa
     2101 ctccaccact tggcagatag agattagtaa tcttacctgg acttggagca aagttaaagg
     2161 ctgggttttc tgcattgata cgacactcga tggcatgacc gcgtaggaca atatcttctt
     2221 gcttaacaga caaaggctga cctgccgcaa tgcaaatctg ttccttaacg atatcaacac
     2281 ctgaaacaaa ctctgttact ggatgttcta cctgaacacg agtattcatc tccatgaaat
     2341 agaaattgct acttgcttca tcaagaagaa attcaatggt tcctgcattc tcatagccaa
     2401 caaactctgc cgctcgaaca gcagcagcac ctatttcatg acgcagcgtt tttccgattg
     2461 caatcgaggg actttcttcc aaaacctttt ggttattcct ttgaagagaa caatcccgct
     2521 cacccaagtg aatcacatgt ccatgctcat cacctaggat ttgaacctca atgtgccgag
     2581 ctggatagat aacccgttct atgtacatgg caccattgcc ataattggcc ttggcctcac
     2641 tagaggcagt ttcaaaggca gaaacgaggt catctggttt ttcaacctta cgaatccctt
     2701 taccacctcc acctgctgaa gccttgagca taacaggata gccaattttt tcagcaacaa
     2761 tcaaagcttc ttcagagtta tgcacttctc catctgaacc tggtataaca ggcacacctg
     2821 ctttaatcat ctgagcacgc gcattgatct tatcccccat catatccata acatgaccag
     2881 atggaccgat aaacttgata cctacttctt cacacatggt cgcaaatttg gaattttcac
     2941 tgagaaatcc aaaaccaggg tgaatagctt ctgcctcagt caagactgca gctgatagaa
     3001 ctgcattaat attgagataa gactctgttg ccttgccagg accaatacaa actgcttcat
     3061 ctgccaaaag cgtatgaaga gcttccttat cagcagttga ataaaccgct accgtcgcaa
     3121 tccccaattc acgtgccgca cggataatac gaaccgcaat ttcaccacga ttggcaatta
     3181 aaatttttcg aaacatggag aacctcctta gttcccaatt gcaaaagtaa ggataccact
     3241 ggctgcaagc ttgccatcca cttcagcctt tgcttcaacc acagctatgg tgccacgacg
     3301 ttttacaaaa gtcgctgtca taaccaattg gtcgcctggt acaacttgct tcttgaactt
     3361 aaccttgtcc ataccagcgt aaaagaccag ttttccttta ttttcaggtt ttgataactc
     3421 taacacacca gcagtttgcg ccaaggcttc cataatcaga acacctggca taactgggta
     3481 ttgaggaaag tggccgttaa agaaaggttc gttaatggtc acatttttga tagcaacaat
     3541 ggtatcctcg ctcacttcca agacacggtc cactagaagc ataggataac ggtggggaag
     3601 agcttctttg attccttgaa tatcgatcat ttgatacgta ccaatccttt accaaactca
     3661 accatttctt cgttagagac gagaatttcc gttaccacac catccttagg agctgggatt
     3721 tcattcatga ctttcatggc ttcgataatt accaatgttt gacctttttt gacactatca
     3781 ccaactgtaa cgaaggcagg tttatctggt ccagcagcca agtaaaccac tccaacaagt
     3841 ggactctcta caagatttcc ctcagtagcc acacttgctt cagctggagc tggaacttct
     3901 tctgctacag tctctgctgg agcagatgta ggagctactg gactcggtgt tgctagaacg
     3961 ggtgctggag cgacttgagt tgcaacttca ggcacaggtc tcgcttcatt cttgctaaac
     4021 tgcaactcat ccgtcccatt tttataagaa aattctctca aacttgactg gtcaaattga
     4081 gtcatcaagt ctttaatatc gtttaaattc atacttatct attctcccaa cgtttgaaag
     4141 caagaactgc attgtggcct ccaaaaccaa aagtatttga aatagcgtat ggaatttctt
     4201 tctccaagcc ttgtccataa acgacattag cttcgatata atctgatact tcacttgtcc
     4261 cagctgtcat tggtacaaag ttatggcgca tagcttcgat ggtgacgata gcttctactg
     4321 cacccgcagc ccccagcaaa tgtcctgtaa aagacttggt tgatgataca ggtacttcct
     4381 taccaagaac agctacgata gctccacttt ctcctttttc attggcagga gttgacgttc
     4441 cgtgagcatt gacataggct acttgctctg gagaaatctc agcttcttcc aaggctagtt
     4501 tgatggcctt gatagctccc tgaccttctg gatgtggaga agtcatgtgg taggcatcac
     4561 aagtatttcc gtaaccaacc acttcagcca ggatagtagc tccacgtttt tcagcgtgtt
     4621 caagactttc taaaaccaac atccctgaac cttcacccat aacaaaccca ttgcgatcct
     4681 tatcaaatgg gatcgaagca cgagttggat cctctgtagt agagagagct gttaaggctt
     4741 ggaaaccagc gatggcaaaa ggtgtgatag aagcttctgt tcctcccacc aacatcacat
     4801 cttggaaacc aaacttaatg gagcggaagg catccccaat cgcatcattt gatgaagagc
     4861 aggcagtatt gatagattta caaacaccgt ttgcaccaaa acgcatggct acattcccag
     4921 aagccatatt tggtaaagct tttggaagag tcattggttt gacacgtttg ggtccttttt
     4981 catgaaggcg aagtacctga tcttcaattt ccttgattcc accaatacca gatgcaacga
     5041 taacaccaaa acgatcccta ttaagagcct ctacatcaag attggcatga tttacagcct
     5101 cttgggctgc atacaaggca tataaagaat agttatcaaa acggttggta tcttttttta
     5161 caaagtattt atcgaacgga aaatcttgga tttctgccgc attatgcaca tcaaagtcac
     5221 tatgatcaaa ttttgtaatg ccaccaatgc cgattttccc agttgctaaa ctattccaaa
     5281 attcttctgg tgtatttccg attggagatg ttactccata acctgttacc actactcgat
     5341 ttagtttcat tcttttcacc tctagctttc gctacatact taagccacca tcaatggcaa
     5401 ccacttgacc agttagataa tcttggcctg ctaaaaatac tgtcaaatct gcaacctgct
     5461 ctgcctgccc aaattctttc atcggaatct gagctagtgt agcttcctta atcttatctg
     5521 ataagatagc tgtcatatca gactcaatca ttcctggagc aatcacattg actcgtatat
     5581 tccgactagc gacctcacgt gccacagact tggtaaagcc aatcaagcca gccttagaag
     5641 cagcatagtt agcttgacca atattcccca tcaaaccaac aacactagac atattaatga
     5701 tagcaccttc tctggctttc atcatcggtt tcaagactga ttgtgtcata ttaaaggcac
     5761 cagtcagatt gaccttgagc actttttcaa aatctgcttc tgtcatctta agcataagag
     5821 tatcttgggt gatccctgca ttgttgacca aaacatctac tgaacccagt tctgcaatag
     5881 cttgatcaat catacgctta gcgtctgcaa aatctgatac atctcctgaa atgggaacca
     5941 ccttgatacc atagtttgaa aactcagcga gcaattcttc tgagattgcc ccacgactgt
     6001 ttaagacaat gttggctcct gcttgagcaa acttgtgggc gatggcaaga ccaattccac
     6061 gactcgaacc tgtaataaag atatttttat gttctagttt catttttttc ctttcaaaac
     6121 ttctacttat tttagtctat ttttctaaaa gtgctactaa actcgcttga tcttccacat
     6181 gagctaagtg agcagttcga tcaatttttt taacaaaacc tgacaaaact ttccccggtc
     6241 caatctcgat aaagttgctt atgcctgctt cttgcatgac cccaatactt tcatagaaac
     6301 gaacgggttc cttgacctga cgcgtcaaga gctgagcaat gtcctctttt tgcatcacag
     6361 cagcttctgt attgccgact aggggacaag taaaatctga aaaacttacc tgagctagag
     6421 tttcagctag tttctggcta gcaggttcaa ggagagcggt gtgaaaggga cctgacacct
     6481 taagaggaat caagcgtttg gcacctgctt cttgcaaaag ttcaaccgct cgatcaactg
     6541 caaccacttc tccagcaatg acgatttgtg caggtgtgtt atagttggct ggagtaacca
     6601 ctccaagttc agaagctttt tgacaggctt cttcaatgac ctctactggc gtattgagaa
     6661 ctgctaccat cttgccagag tcagcaggag ccgcttcttc catataggct ccacgcttag
     6721 ctaccaaggc aaccgcatct tcaaaatcca aggcgccgct tgccaccaag gcagagtatt
     6781 ctccaagaga caaaccagca accatatcag gctgatagcc cttttcttgc aataaacggt
     6841 agatagcaac cgaagtcgct agaatggctg gttgcgtata gcgggtctga ttgagtttgt
     6901 cctcttccgt atcgatgaga taacgcaaat cataacctag cacctgactc gctcgatcaa
     6961 tcgtttcttt aacaatcgga tactgatcat agaaatcccg tcccatccct agatactggg
     7021 caccttgacc agcaaataaa aaggctgttt tagtcatttc ttacaactcc tgcccagcga
     7081 gaggcttctt cttgaatttt cttagcggct ccgtaataca aatcttttag gatttcttca
     7141 gctgtttctt ctttagaaac aagccctgcg atttgacctg ccatgacaga gccaccctcc
     7201 acatcaccgt gaacaactgc tttggctagg gcacctgctc ccatttgttc aaagatttct
     7261 aaatcaggat cttcctgctt aaaggcatct ttttcagcca gttcaaaatc tctagtcaac
     7321 tgatttttaa tagcacgaac agcatgacca aagtgctgag ctgaaatcgt agtgtcaata
     7381 tcccttgctt ttaaaatttt ctccttgtag tttggatggg cattcgactc ttttgcaact
     7441 acaaaccgtg tccccacctg tacagcctct gcacctagca taaagccagc cgcagcacct
     7501 tcaccatccg caattcctcc tgcagcaata acaggaatag atacagctgt ggctacctgt
     7561 cgcaccaagg tcatggttgt taatttaccg atatgccccc cagcttccat tccttctgca
     7621 ataacagcgt ctgcaccgat tttttccatg cgtttagcta aagcgacact aggaacgacg
     7681 ggaataacga ttatcccagc ttcatggaaa cgttccatat acttgcttgg atttcctgct
     7741 cctgttgtga caactttaac accttcttca ataacgagat ccacgatatc ttccacaaag
     7801 ggagataaga gcatgatgtt gaccccaaag ggtttatcag tcaatgattt gattttatca
     7861 atattggcct tgacaacttc tttcggggca tttcccccac cgataattcc taatcctcca
     7921 gccttggaaa cagcccctgc caaatcacca tcagcaaccc aggccatccc tccttggaaa
     7981 ataggatagt caatcttcaa taattctgta atacgcgttt tcatagtgcc tccaaccttc
     8041 cttgcttacg taatagttcg atttaaccat aatttgacag tcaaactatt acctaaacaa
     8101 gagggagtgg gtttctccct actccttcta ctaatattct gcttattttg cttgctcttc
     8161 aacgtaagca accaagtcac caactgtttt caagtcattt tctgcttcga tttggatatc
     8221 aaaagcatct tcgatttctg agattacttg gaacaagtcc aatgaatctg cgtccaaatc
     8281 atcaaaagtt gattcaagtg ttacttctga tgcgtctttt ccaagttctt caacgataat
     8341 ttcttgtact ttttcaaata ctgccatgat aggactcctt taaaataaat agttttttta
     8401 taacaatgtg ttcaccacat gattacctaa attgtaagaa tgagcgtgcc ccaggtcaag
     8461 cctccaccga agcctgatag aagaacagtc tggctaccat ctaaagggat gagaccttgt
     8521 tctacacact ctgaaagtaa aatcgggata ctggctgcac tggtattgcc atattccatc
     8581 atattggctg gaagtttggc tcggtcaaca ccaatttttc tagccatctt atccaaaata
     8641 cggtcattgg cttgatgaag tagcagataa tccaagtctg tcacctctat aggagattca
     8701 tcaatagtct gcttgataga cttggctaca tctcgaatgg caaaatcaaa gactgtgcgt
     8761 ccatccatct tcaaaaatga atctgcactt tcttgatctg aaaatggaga atgcaaacct
     8821 gaatgcccat aagttaaaca ctcgctgcga cttccatcgc tattgagact ctcagctaag
     8881 aaatgctctt gctcgctagc ttctaacaag acaccaccag caccatctcc aaacaacaca
     8941 gctgttgatc gatccgacca atcgactgcc ttagagaggg tttcactacc aatcaccaag
     9001 cctttttgaa agcgaccaga agcgataaac ttttcagcag ttgaaagagc aaatacaaat
     9061 ccactgcaag ccgcggttaa gtcaaaagca aaggctttat tagcgccaat attagcttga
     9121 acacgagcag ctgtagaggg catcatcgaa tctggagtaa tggtagctag gatgataaaa
     9181 tccagttctt ctcctgttat tccagctttt gccatcagtt tcttagcaac ctctgtagcc
     9241 aaatcactgg tagattctgt tcttgaaata tgcctttgtc gtattcccgt tcgacttgaa
     9301 atccactcat cattggtatc cataatctga gccaagtcgt gatttgtaac cacttgctct
     9361 ggcacataat gagcaacctg acttattttt gcaaaagcca ttatttcaaa tcctccaaaa
     9421 attggtaaag attagtcaag cctttaccca tgacagcaat ttcttcctcg ctcatgccat
     9481 caataatttt ttctaccatg gccttgtgga agcgtttatg cagtctatga atcaagcgac
     9541 ccttctttgt caaatgcaga tgcaccacac gacgatcctg ttctgaccga actcgctcaa
     9601 tgtagccctt acgctctaaa ttgttcaaac ttgtcgtaac agttccaaga gttaccatca
     9661 actcttttga cacttgactt ggagtcacat ctggagcctt accgatgaca tcaatcgtat
     9721 gcatttcttt gatggagata tccttaaaac gactacctct caagttcact tcctcaatta
     9781 caaggacatt gttaaatata gatgttaaat attcattgat tcgttggtag tccatttttc
     9841 atatccctcc ttcttcaaaa aacttttaca atcaaaacat ttgacagaaa gaattatatc
     9901 aaaattcaaa gaatgatgca agtatttttt attttcctgt aaatttaggt cttcttctct
     9961 ccgaatgagc tcgcactcct tctttgaaat cctctgtttg agctaaggat ttctgtaggt
    10021 tcagttctaa agtagcgtaa ccctgccagt ctttaaattg gctctcccaa accaacttct
    10081 taatggcagc ataggaatta cttgaagcac gtcttaattt tttaagaagc tgttctctcg
    10141 tcttttcaag tttttcagct tcagaaacgc ggtaaaccaa cccccattct agggcttttt
    10201 ctgccgttaa agcctctcct gtcatagcta attgagcagc acgcgtcaca ccgatactgc
    10261 ggctcaagag atgaattccc cctgcatctg gagccaaacc aacaccaaca aaggcttgga
    10321 taaacttagc cttatccgtc gccaaacaga aatctgccgc aacagccata ttcgctgcgg
    10381 cacctgcaac agcaccgtca acttccatta aaacaggttt ggctatttgc ttgattttat
    10441 aagaaatcgt attgaccaac tctgcgattt ttgtcaatga tggaatatca tcctcatcca
    10501 ctgcccgctt catctctacc aaatctcccc caactgagaa gacctttcca ttggcattga
    10561 ttaagataaa atgcacagct ggattttctt ctgccaaagt cagagcttct aaaatctcct
    10621 cacacatggg aatatgaaaa ccatttgcga cctcaggacg gttcaaggta aggattgcca
    10681 agtcctcttc aagttgataa ataatgtgtt ccatcagggc tcctttttat tttataagca
    10741 gtttatactc ttcgaaaatc tctttaaacc gcgtcagctt ccatctgcaa cctcaaaaca
    10801 gtattttgag ctgacttcgt ctagcttctt agtttgctct ttgattttca ttgagtgtta
    10861 aattatttca ctgtcaaagt atatcttttt taggagcaga aagcaagaaa aaatcgactg
    10921 aaagtgtctc agccgataag aaaggctcgc tttaggctag aaagcatctt attttcttca
    10981 ttgaaaaaag cctttctttc tgctataatc gtataaaata cttactttag gagttcttat
    11041 gaaagttgtt aaatttggag gtagttctct tgcctctgct agtcaattag aaaaagtttt
    11101 aaacatcgtc aaaagcgatt cagagcgtcg ttttgtagtc gtttctgcgc ctggtaaacg
    11161 caatgctgaa gatactaagg ttacggatgc cctgattaaa tactaccgcg actatgttgc
    11221 gggtaacgat attagcaaga gccaaagctg gattatcgac cgctatgctg ctatggttag
    11281 tgaattggga ctaaaaccag ctgtgctaga aaaaatttct aaaagcattc acgccttggc
    11341 cactcttcct attgaagaaa atgaatttct ctacgatact ttcctagcag ccggtgaaaa
    11401 taacaatgcc aaattgattg ctgcctactt taaccaaaat ggtatcgatg cacgctatat
    11461 gcaccctaga gaagctggga ttgtggtcac aagtgaacct ggtcacgctc gcatcattcc
    11521 atcaagttat gacaagattg aagaattgac aaacaccaat gaagtccttg tcattcctgg
    11581 tttctttggt gtcactaagg aaaatcaaat ctgtactttc tcacgtggag gttctgatgt
    11641 tacaggttct atcattgctg ctggtgtcaa agccgacctc tatgaaaact ttacggacgt
    11701 tgatggtatc tttgcagccc accctggtat tatccaccaa ccacactcga ttcctgagtt
    11761 gacctaccgt gaaatgcgcg agttggccta tgcaggcttc tcagtccttc atgacgaggc
    11821 tcttcttcct gcctaccgtg gaaaaattcc tctggttatc aagaatacca acaaccctga
    11881 ccatccaggt actcgtatcg ttctaaaaca cagtaatgat gaatttccag ttgtgggaat
    11941 tgctggtgac tcaggctttg tcagcattaa catgtcgaaa tacctcatga accgtgaggt
    12001 tggatttggc cgcaaggttc tgcaaatcct ggaagaactt aacatcggtt gggaacatat
    12061 gccaacaggt atcgacgatc tttctatcat tctccgttct cgccaactaa ctcccatcaa
    12121 ggaagaagaa atcctgcgtc agttggttca aaaggctaaa gtagatcatg cagaaatcga
    12181 acacgacctt tctatcatta tgattgttgg tgaaaaaatg aagagccaca tcggagtgac
    12241 tgctactgcg acacgcgctc tatctgaaaa caaaatcaac atccagatga tgtctcaagg
    12301 ttctagtgaa gtttctatta tgtttgttgt caataaagac caagagaaag cagcaattaa
    12361 agccctctac aatgcctttt ttggagaaag taaggaagac taagcatggc ccaatcactt
    12421 aacaaaacag tactcctcag tacgacaggc acttcctacc tctctatcgc tgggaaagtt
    12481 gggaaattcc ttgtcggaga tcaggctttg gaattttacc cagatgtcaa tgtcgaacaa
    12541 tttatccaga ttccttggag ccatatcaat cagattggag ccaatgtcac tggacgcaaa
    12601 atcagccgcc acttcgaagt ctttacagac aaaggaaaat tcctctttgc ttcaaaagac
    12661 tcaggtgcca ttctcaaaat tgcacgcgaa aaactaggca atgacaaggt cgtcaaactt
    12721 ccgaccctga ttcagacaat tagccaaaaa tttaaaaatc tatttgcaaa aaagtagaaa
    12781 ctttagtata atcatagcaa cggataagta ggttatcttc ttctttcaga aagtctgcgg
    12841 gtactgcgag cagataggag ggataggtga aattctaccg ttagtaacaa ttacatacac
    12901 aatcaagtgc acacctgtga agttggatgg aaccgtggcc ctgccactcc aacgttttgt
    12961 caggcgtgct tttttcataa aggagttctt atgttagata tcaaacgtat tcgtacagat
    13021 tttgaagctg tcgcagaaaa attagctaca cgtggtgtag atgctgctgt cttgaatgaa
    13081 atgaaagaaa tcgatgctaa acgtcgtaac atcttgatca aggttgaaac tctcaaagca
    13141 gaacgtaaca cagtttctgc tgagattgcc caagctaagc gcaacaagga aaatacagat
    13201 gacaagattg ctgccatgca aaatctatct gctgagatta aagccttgga tgctgaattg
    13261 gcagaaatcg atgctaaatt gacagaattt acaacgactc ttccaaatat cccagctgac
    13321 agcgttcctg ttggggctga cgaagacgac aatgtggaag ttcgccgttg gggtactcca
    13381 cgcgagtttg acttcgaacc taaagctcac tgggatctcg gtgaagacct tggtatcctt
    13441 gactgggaac gcggtggtaa ggtaacaggc gctcgcttcc tcttctataa aggcctcggt
    13501 gctcgtttgg aacgtgctat ctacaacttt atgttggatg aacatggaaa agagggctat
    13561 actgaagtca tcacacctta catagtcaac catgattcta tgtttggtac tggtcagtat
    13621 ccaaaattta aggaagatac ttttgaactc agcgatacca actttgtctt gattccaact
    13681 gctgaagttc ctctgacaaa ctactaccgt gatgaaatct tagacggcaa agatcttcca
    13741 atctacttca ctgctatgag tccgtcattc cgttctgagg ctggttctgc cggtcgtgat
    13801 acgcgtggct tgatccgttt gcaccaattc cacaaggttg aaatggtcaa atttgccaaa
    13861 ccagaagaat cttacgaaga attggaaaaa atgacagcca acgctgaaaa cattcttcaa
    13921 aaactcaacc ttccataccg tgtcgttgct ctctctactg gagatatggg cttctcagct
    13981 acgaagactt acgacttgga agtgtggatt ccagcacaaa acaattaccg tgaaatctca
    14041 agctgttcaa acacagaaga tttccaagcc cgtcgtgccc aaatccgtta ccgtgatgaa
    14101 gcagatggca aggtgaaact ccttcatacc ttgaacggtt ctggacttgc agttggacgt
    14161 acagtggctg caattcttga aaattaccaa aatgaagatg gttctgtgac catcccagaa
    14221 gcacttcgtc catacatggg tggagctgaa gtcatcaaac cataaaaaat aaggtttagc
    14281 tatttctagc tagacctttt ttcgtaacca aatcagataa gcacctagta caaagaataa
    14341 aatagttagg catataatgg tttcagccaa taccaggtaa tccagaaatg gaagtttcaa
    14401 aattccctga gccatcttga gcgaggtcgc tgtgataatg gttgggaagg tgagggctga
    14461 gaaggctggt tgaaaacctt gttttaaaat gttgggcaga cgagttaaaa caaagaaaaa
    14521 gaaggattga gaagccaaaa tcatgacaat caagccccaa gtcggcaggc tggttcctcc
    14581 tactcgaact agagaagcca agagtagaga gaaaggagca cagtagattc cttcttgtcc
    14641 aagcaaggct agtgggagtg gatgtttctt taaatcgcta taaataaggg gatagagata
    14701 gaaggtcaag agaaaaccaa aactcaaggt cgcataggca atttcgataa tacctaccag
    14761 aggataggtc aaggcagcca ctgctatccc cacatagaga accgtccaac ttggagtggc
    14821 atgaaccctc cgccctggac aagcaaactt gatggtaaaa ccagcaatca aggtcaaatc
    14881 caagagaaat gaaaaccacc aaatcccttg tgctaccaaa ggaagataag agaatacgcg
    14941 aaagacatag gtcgataaaa tcatcccagc cataggaaag gttgccattc ctgacaaaag
    15001 agggggcttg gtcaattctt gcttggtttc tttccaatta aagagatgca gaattagaaa
    15061 gtaaatccat aaaaccaaac cagtcagact aaaaagatgg gatagaaccg gcaacgtatc
    15121 taaaataaga tttccagctc ctgccaaacc tagcaaacaa cctgaaaata ctaaggggag
    15181 ttttttcatc ctaacctcca ataatcatgt tagtttcagt ataacataaa agcgcttaaa
    15241 tgaggattta aaaaaacgaa gtccgcttat ttcagacttc attttactca gatatgaatt
    15301 aggcataagg ttgcaattct ggattaattg gtgtattagc taagttgttg gcatagttac
    15361 agaggattgc taggctgaca ccaaaaacca catccaaggc attttgttga gtgtagccag
    15421 cttctaaaaa ctcagacaag gcttcatctc ctacacgacc cttggtattg ataactgcca
    15481 aggtaaactt agctagggta tccaatttag gatctgtttc aattggagta cgattgcgaa
    15541 gagcttgaat caagtcatca ttcatctgga tttgtttgat ggaaaaggct gtgtgacctg
    15601 cgacacagaa ggcacaacca ttggtcacgg ctgccgtgat ttgcaccact tcacgctcaa
    15661 cgggtgtcag gctgttgcga cggtggatag atgagacaat ttggtaggct tctaaaacag
    15721 tcggggcatt ggccaagaga ccgattaggt tgggaatata gccattgttg tctttttcta
    15781 ctgtttcaag aatttctttc acttctgctg gtgctgactc tactgtatgg atagtaaatg
    15841 ttgtcataag atacctcttt tcttattatt gacactaata ttattggaaa atcttataaa
    15901 atcctgattc ctaagtttat ctaagataaa gctttattct ctcataagat tttcgttgtt
    15961 atattagttt atcacacttc caatcacttg cataatatat attatatatc aggctgataa
    16021 aaattattta taggcaaaaa aatcacacga gctgtgtgat tccattattt gtcaaaatac
    16081 tttttagttt cagcaataac gactggcgac aagaccaaga gggcaatcaa gtttggcaga
    16141 gccatcaagg cgttaacgat atctgcgata atccagacca tatccaactc gataaatcct
    16201 cctaacaaga ccatgagcac aaaaaccaca cggtagagcc agataaagcg aaccccaaag
    16261 aggaactcaa aacagcgttc tccgtaatag ttccaaccta gaatcgttgt aaaggcaaaa
    16321 agcacaagga agatggtcaa gagggcaggg ccaaagtgtg aaaagactgt tgagaaagct
    16381 gactgagtca aggcaacccc attcaagtca ccactccaaa ctccagttac caagatggtc
    16441 aaaccagtta gagtacaaat gatgagggta tcaataaagg ttcctgtcat ggaaatcaaa
    16501 ccttgctcta ctggttcatt tgtcttggca gctgcagctg caataggagc agaacccaga
    16561 ccagattcgt ttgagaacac accacgcgcc acaccatttt gaatagccat ccgaacgcta
    16621 gcaccagcaa atccacctac cgcagcaagg ggactaaaag ctgaggtaaa gactaaagcg
    16681 attgtgccag ggatttttcc gatattaaag aaaataactg taagagttcc taagatataa
    16741 atgatggcca taaaaggaac aacagtagtt gaaaccttag aaatagactt gagtccacca
    16801 aagactgcaa tcgctacaaa gacagacaag acgagagctg tgatggctgg cgaaatcgtc
    16861 gttgtatttt ggatagattc tgtaatcgag ttgacttggg tgaaggttcc gattcccaag
    16921 agagcaacca atactcctgc tactgcaaac aaaacagcaa gtggtcgcca cttttctccc
    16981 atccctagaa ggatataatg catgggacct cccgctactg caccatggtc gtccttggtg
    17041 cggtatttga tggccaagag tccttccgca tacttggtag ccattccaaa gaaagccgcc
    17101 atccacatcc aaaatagagc tcctggtcca ccaaccttga tagccgtcgc aactcctatg
    17161 atatttcctg ttccaacagt tgatgccaag gctgtacaca gagctgcaaa actggataca
    17221 tcaccatgtc ccttatcctg gataaaaata agctgaaagg ccttgggcag acgcaaaacc
    17281 tgcaagagtc ctagccgcat ggttaggtaa atccctgttc cgaccaataa aatcaagagg
    17341 ggcggtcccc aagcaaaatc atcgattgat ttaagcaatt ctaacatttc cttctcctat
    17401 cgtttcaacc ccaaaagaaa gagcacatgc aagatacatg tactctggaa tgcttagata
    17461 aatgctaaaa agcggtctat cctagctctg tccttttacc tgagagtttg agcagttgcc
    17521 tgccttgccc cttcggtgcc tttacggtct ctccagagtt ccgtccattt acagtcatgg
    17581 aaaatcaaac gattccccac ttctattaaa cttcattcgg tgttggtatt taattgattc
    17641 taattttaca aaaaatgttg gcttttgtca atgtgtttat tagtaaaaat tagttcaaca
    17701 gtttttactt tataaagtcc agaatactgc tatcctttaa aagtgacaat agtcgcacca
    17761 ctgcctccag cattttgtgg ggcatagccg aaactcttga catgtttgtt tctttgcaag
    17821 tatttggtaa ctccttcacg gatgactcct gttccgatac catggatgat atcaacttga
    17881 gccatattgt taagcaaggc ttggtcgatg aaggcatcta gctcattcat ggcttcttca
    17941 tagcgcttgc ctcgaagatc cagtctagct tgaggtcctc gcccagaagt tcgtttcaca
    18001 acattgacct gtttcttctt gactggtttt tcttgctggg cttgaacaag atcaaactct
    18061 ttctcttcca aggtcatctt aatcaagcca acttgggctt cccagcgacc gtccttgagt
    18121 tgactggtca aggtaccacg ctgaccataa ctgagaacca cgatatcatc tcccaccttt
    18181 ggagctcgtt ttttcttggc cttttgaagg accttatttt tagacaagtc cactttttca
    18241 ggagccaatt ttttcaactt ggccttggct tcaatgattt cgtggggctt gagttgggat
    18301 ttactgtgga gatttttgag aatctggtca ctttcactta gggccatatc cacaatctca
    18361 gcagcctgtt cacgcgcctt gttaagctcg gtttcctttt cacgattaag ctcgttgtag
    18421 agttttttta gcgcacggtt catcttgaga ttttcttgct ccacctcacg gatattgtcc
    18481 aagcgtttgc ggctttccag cgtctgctct tctaattgct caatgatacg attgacgtca
    18541 ttgtcctgat cgatctgctg actggcatct cctacgataa cttcagatag gcctagacgt
    18601 ttggcaattt caaaggcatt acttcggcca ggaacaccct gcataaagcg ataggtcggg
    18661 cgaagagttg cagtatcaaa ctccatactg gcattttgca caaaggctgt ctcaataccg
    18721 taggccttga gttctggata gtgggtcgtc gccatggtct tgatttgacg caggcgaagg
    18781 tcctccagaa tagccatggc aagggctgct ccctcttggg gatcagtacc agcccccaat
    18841 tcatccaaaa gtaagagtga atgttggttg accttgccaa gaatatccac gatattggtc
    18901 atatggctag agaaggtaga caagctctgc tcaatagact gctcatctcc aatatcagca
    18961 aaaatttctt caaaaatacc aacacgactt cccttgtctg ctaaaatcgg caatcctgac
    19021 tgggccatga cctgtgtcaa gcccagagtt ttgagcatga tggtcttccc acctgtattg
    19081 ggacctgtaa tgacaatagc tgttaaatct tgaccaaaat agacatcatt tgcgacggca
    19141 tttttgacca aaggatggca gacatggagc agttgaatct cttgattttc tgacagctga
    19201 ggcacgactg cttgtctttc ttggataaat cgaaccttgg cacgaatcaa gtccagatga
    19261 ccgataatcc aagcatcatt agcaatctca gccgcatgag ggcggacacg ctcagaaatt
    19321 tcttggagaa tgcgaagcat ttcatagcgc tcatctgctc gcagactagc aatttcttcg
    19381 ctcagtttga ctacctcacg gggttcgata tagacggtgt ttccactagc agaaatatca
    19441 tgaacgacac ctgcaatctt attgcggtag gtgtttttga ctggtaaaac cttacggcca
    19501 tttctgctag caacaattcc ttccgtcaac atctgcgctt tttgcttgag caagtcttgt
    19561 aaaacatcgc gtacctgact ctcgctatca tgtatttttc gacggattcg cgccaattct
    19621 tcactggcaa aattttcaat gaaacccgca tcattaaagg cctgaagatt tccttgtaat
    19681 tgcggaaaat catgtaattt ctcaaaccaa agggctaatt cttccaagct gacattttcc
    19741 agattggcgt aaaaactttg aagttctcgg ctggcaagaa gcacgcgttt caagagtagg
    19801 aactcctcga tattgagatc cgctcccatc tccaacctct tgcagactcc tgcaatttcc
    19861 ttagttgaga gaatagtaaa atgcggttgc tcgacgaaaa gagcctgcat ttccttcatc
    19921 tcagcaaaag cctgtttgat tttatctgct ttggcagtcg gagccagttg tctcaattgc
    19981 tccaagccct gctcggtcaa caaatgaggc tcaaacaagg ccttgacctt atcgaactct
    20041 aatgtttcta atattttctt attcatcttt atttccttgt ctttgaatgt ctaggtgtgg
    20101 tagcttttta cattctgata gattcaagtc aaatctgtaa acaaagggct aggaaaactc
    20161 ctgccctttt tatccgatta aatttgtcac ccagatttgt ttgagccaac tggttgttat
    20221 cggtatgctc tggatgatgt gttttgcgac gatactcttt tcaagaggat tttgtataac
    20281 tgccatgggg atggtcgcca agattgtcaa ggccatttgc aagacaaata aggtcaccaa
    20341 catggacaag atacctgctg aaacttggaa caacttacca cccagttttt tgctaggaat
    20401 caagtgtaag agaagaccaa gcaaacgacc aatgctatag acaatcccaa atacaagcaa
    20461 gtagccgata cctgcataaa agaccttatc cagctgaaag agttgatccg atgggaaaaa
    20521 gaaagtcccc tgaccttcct gcgaatttgc ataagggagc aataaatgga attgctctcc
    20581 aagcccctta taaaactgac cagccataaa agccgatgcc atggctgaaa tcaggtaata
    20641 aatctgtaag agcaggcctc tccgatagcc gatataaaat ccccaaacca agaccaatag
    20701 aagaaggaat gaaatcataa ggaatcctca atcttgctct gttcttgctt acaagtcaca
    20761 agcttgtgac ggagttcttc tagctcttgc tccttatcgt caaattcaat ctcacggctg
    20821 agctgagttg ataaacagtt gactgccaac aaaagagcga ttgtttcatc atctgcgcta
    20881 ggcatttgtt ctttaattgc ttggtatttt tctgtcgcaa ccttagcgat ttcctccata
    20941 aaaaggttat catgttcact tgtcaaggtt aacgattttt tcccgaatgt aaatttgaat
    21001 cgatttagat ttgccataaa attcacctca cgatattata ccaaaattcg ctaattttgt
    21061 cagtttttac aaattttact gcttttgtgg tacaatagaa actatggcaa gtatcacact
    21121 cacaccaagc gaaaaggata ttcaggcttt tcttgaacac tatcaaacca gtctggctcc
    21181 tagcaagaat ccctatatcc gctacttttt gaaactacct caagcaacgg tttctatcta
    21241 tacttctgga aaaatcttgc ttcagggtga aggggctgaa aaatacgcca gtttctttgg
    21301 ctatcaagct gtagagcaaa ccagcggaca aaatcttcct ttaattggga cagatgaggt
    21361 gggaaatggt tcctactttg gtgggcttgc agttgtggct gcctttgtca cacctgacca
    21421 gcacgacttt ttacgaaaac tcggtgtggg agattctaag actctgaccg accaaaagat
    21481 tcgtcagatt gctcctattc tcaaggaaaa aatccagcac caggcactcc ttctctcacc
    21541 cagcaagtac aacgaggtca tcggagaccg ctacaatgct gtttcggtta aggttgccct
    21601 ccataatcag gctatctatc tcctccttca aaaaggtgtt cagcctgaga aaattgtgat
    21661 tgatgccttt accagtgcta aaaattatga caagtacttg gcacaagagg ccaatcgttt
    21721 cagcaatcct atcagcttag aagaaaaggc tgaaggcaaa tacttgtctg tcgcagtttc
    21781 ttctgtcatt gcgcgtgatc tctttctgga aaatcttgaa aatctgggac gagaactggg
    21841 ttatcagctt ccaagtggag ctggaacggc ttctgacaag gtggctagcc agattttgca
    21901 agcctatggt atgcagggac tcaacttctg cgccaaattg cactttaaaa acactgaaaa
    21961 agcgaaaaaa cgcttagaaa ggtaagttat gaattcattt aaaaatttct taaaagagtg
    22021 gggactgttc ctcctaattc tgtcattact agctttaagt cgtatctttt tttggagcaa
    22081 tgttcgcgta gaaggacatt ccatggatcc gaccctagcg gatggcgaaa ttctcttcgt
    22141 tgtaaaacac cttcctattg accgttttga tatcgtggtg gcccatgagg aagatggcaa
    22201 taaggacatc gtcaagcgcg tgattggaat gcctggcgac accattcgtt acgaaaatga
    22261 taaactctac atcaatgaca aagaaacgga cgagccttat ctagcagact atatcaaacg
    22321 cttcaaggat gacaaactcc aaagcactta ctcaggcaag ggctttgaag gaaataaagg
    22381 aactttcttt agaagtatcg ctcaaaaagc ccaagccttc acagttgatg tcaactacaa
    22441 caccaacttt agctttactg ttccagaagg agaatacctt ctcctcggag atgaccgctt
    22501 ggtttcgagc gacagccgcc acgtaggtac cttcaaagca aaagatatca caggggaagc
    22561 taaattccgc ttctggccaa tcacccgtat cggaacattt taagaaacct aagaggccga
    22621 gaatcaccaa tctcagcctc ttcttctatc gtgagaagtg attgtttatt gacaaatact
    22681 caatgaaaat caaagaacaa actaggaagc tagccgtagg ctgcttaaaa cagtgttttg
    22741 aggttgcaga tggaagctga cgtggtttga agatattttc gaagagtata aaataaaaac
    22801 aatcccaact ctcacctatt catgcaaagg aattttatgg aagtttattt ttcaggaacc
    22861 attgaacgga ttatttttga aaatcccagc aatttttatc gcatcctcct cctagaaatc
    22921 gacgatacgg acgcagagga ttttgatgac tttgaaatca ttgtcacagg aaccatggct
    22981 gatgtaattg agggcgaaga ctatactttt tgggggcaaa ttgtccagca ctccaagtat
    23041 ggagaacaac tgcaaatcag tcgttatgat cgcgcaaaac caactagtaa gggcttggtc
    23101 aagtactttt caagtagcca tttcaagggg attggtctca agacagctca gaaaatcgtg
    23161 gatacctatg gcgaaaatac cattgacgaa attttgcaac acccagaaaa gttagaaggc
    23221 atcgcaggac tctctgccaa aaatcgcgag gctttcgtct ccactctccg tctcaactac
    23281 ggtacggaga tggttttggc caaactagcc aactacggca ttcccaacaa actagccttt
    23341 cagattcaag acttttacaa ggaagaaacc cttgatgtgg ttgaaaatta tccctaccaa
    23401 ctggttgagg atatcaaggg tttgggcttt accattgctg accaactagc tgaggaacta
    23461 ggcatcgaaa gtcaggctcc tgaacgcttc cgcgccggtc tagttcacag tctttttcag
    23521 gcctgtatgg aaacaggaga cacctatgtt gaagcacggg atttgctgga acaaaccctt
    23581 actctccttg agtcttcccg tcccgtggaa ctagacccca gccaagttgc ccaagagctc
    23641 tcctacctga tcgaagaaga caaggttcag cagattgata ccaagatctt tgacaacagc
    23701 ctctttttcg ctgaggaagg catccgcagt cacttggttc gtatccttga aaaaggagaa
    23761 cagaagagtc atgatttaga aaccattcaa aaacatatca ctactatcga ggaagagctg
    23821 ggaattgagt atgataacat tcaaaaacag gctatctgtg atgctatcca gaataaggtc
    23881 tttatcctga caggtggacc tggtactggt aagacaactg ttatcaatgg gatcatcgct
    23941 gtttacgccc tgttagaggg acttgacctc aggaagaaaa gcaatctgcc cattcttctt
    24001 gctgctccaa ctggacgagc cgctcgccgc atgaatgaat tgacaggttt gcctagcgcg
    24061 accatacacc gccacttggg aatgacaggt gacgatgata ccagtcatct ggaagattac
    24121 ctagatgctg actttatcat cgtggatgaa tgctctatgg tagatacttg gctggccaat
    24181 caactcttct ccaatatctc ttctaacagt aagatcctca tcgtaggtga cagtgatcag
    24241 ctaccttctg tcagtcctgg acaggttcta gcggatctgc ttcatattcc tttgattcct
    24301 cagactcgct tggaaaaaat ttatcgacaa agcaaagaat caaccatcgt caccctagct
    24361 agtcagattc gacagggcat cttgccagct gatttcaccc aaaaaaaagc tgaccgttcc
    24421 tactttgaaa ttgctagtgg ccatattcct gccaccattg aaaaaatctt aggtgccgcc
    24481 ctcagaaatg gtattcctgc ccgtgatatc caagttctgg ctcctatgta ccgagggacg
    24541 gcagggattg atgctatcaa tcagctcatg caagacctgc tcaatccccc acaaaaagat
    24601 caactcagtt ttgaagctcc tcagtgccac tatcgtaaga gagacaaggt cattcatttg
    24661 gttaacgatg ctgaaatcaa tgtctttaat ggggatttag gagctatcac agacctgatt
    24721 cctggtaaat acaccgaatc gaaacaagac gagattgtca ttgattttga tggtaatgaa
    24781 gtctcttacc cccgtaacga atggtacaag attcgcttgg cctatgccat gagtattcat
    24841 aagtctcagg gaagtgagtt cccagttgtc atcctaccga ttactagtgc tagccgacgt
    24901 atgctggagc gaaatctcat ctacacagcc attacacgtg ccaaaagcaa actcatctta
    24961 ctaggcgaat tacaagcctt cgactatgct acccaacaca tcggaactgc ccgaaaaacc
    25021 tatctgattg aacgcttcag tgatctactg gagaatgttg aagaaaagca acaagctgtc
    25081 tctgaaacag tcacatcaag tgcctctgaa caatcctaca tcctaaccga agaaaactgg
    25141 gacagcattc cagccatgat tgggattaca gacacagacc tcaaagagat ttttggaaaa
    25201 tagtacatca gaaaacccac ctgaattggt gggttttctg tttattaaga ttattttact
    25261 gttgctgtgc ttgtgatcaa ttcaacagct tttttgatag tgatatcatg tttcaacatg
    25321 tcagctgaaa gcaagttttg aacttgtgca acttccatgt tgtagtctgc tgccaattgc
    25381 tcaacttctt tttgtatttc ttcttctgaa gcatcaaatc cttcagcttt ggcaactgct
    25441 tcgataacaa ggttagtctt agtacgtgac tcagcttctg cttggtattg gttgtgaagg
    25501 tcttcttgag tagttccagt gatttggaag tacatgtcag ggttgatccc ttgacgttgc
    25561 aagttcccaa ggaattcatt tactgaacgg tgaacttctt catggatcat ttcttctgga
    25621 agttctacga tttcagcgtt ttctacagct gtatcaattg ctgcaccttc aactgcatct
    25681 ttgtaagttt cttctttagc agcagccaat tctttgcggt atttttcttt caagtcagca
    25741 agtgtttcaa cttcttcatc aatgtctttt gcaagttcat cgtcaagagc tggaacttct
    25801 ttagctttta cttcgtggat agttgtcacg aatttagctt ctttacctgc aaggtcttct
    25861 gcttggtagt cttctgggaa tgttacgata acatcaacgg tttcgccagc tgagtgacct
    25921 accaattggt cttcgaaacc agggatgaat tgacctgaac caagtccaag tgagaagttt
    25981 tcaccttttc caccgtcaaa ttcaacaccg tcgatagaac caacgaagtc gatcacaaca
    26041 gtgtcgccgt tttcagcagc agcttccttg ataaccaatt cagccaggtt gttgcgttcg
    26101 cgttcgatac gctcttcgac atcagcgtca gttacttctt tttctacatc aactgatact
    26161 tcaaggtttt tgtagtcacc caattttact tcaggttttg taacgacttc agcagtgata
    26221 acccagtctt gacctttttc cattgaagtt acgtcaattt ttggttgggc aaccacttca
    26281 agaccagctt cttttacagc tgcttcataa gcgtttggca aaagtgcgtt cattgcatct
    26341 tgataaagag cttcttcacc aaatttttgg tcgaagatag ggcgtggaag gtgaccttta
    26401 cggaaacctg gaacattaag agatttcttc actgacttga agacacggtc caattctggt
    26461 ttgatttggt cttgagagat agtgaaagtc aagacaccac ggtttgtttc tttgttttca
    26521 aatgatacag acattctgtc atttctcctt aaaatttttt aaatacagtc tattataaca
    26581 ttttctataa ataaaatcta gactaatgca gccaattata gacaacaagc ctttttccta
    26641 actaaaacac tcataaagta tagacaattt tagactactt tggatatctt ttggttagtc
    26701 attttccaaa actaatttat ctaatttttc agttatatag tagattgaaa ctagaatagt
    26761 acatctctac ttctaaaaca tttttagaaa tcgatttgac tgtcctgatc gatttgtcct
    26821 gttcttgttt cattttacta tatagatcgc attcgaaaga attcgttcca aaattgcttg
    26881 gttcgttctt ctcttggacg actaaagaat tgatgggcat tatttttttc aacaattttc
    26941 ccactgtcca taaaaataac atgatgggct acatctctag caaaacctat ctcataaagc
    27001 tgatcaaaaa ctcaatctga ggagaaaatc caataaggct ttccataata aaggacatcc
    27061 tatcaagatt tttgaacacc cgataggctg ccttttcccg attttgaaga ctttttgtcc
    27121 agcttctttt accttataaa taacaacaaa aatcaaagac aagctagaaa gattaccgca
    27181 gataactcaa acagtgtttt gaggttgcag ataagactga cggagtcagc tcaaagcact
    27241 gctttgaggt tgtagataga actgacgaag tcagtaacat atgcctaaga caaagcgatg
    27301 ctaacctgat ttgaagagat tttcgaaaaa gattaaatat actctacaat ttgctcaatc
    27361 aattcttggt cgtctaaacc tgtaacttgt ataacaacat ctttgactga ttgtttagcc
    27421 aacaattcac ctaatcgaat actttcttca tcatttacat cgcgatagtc aaaggcatag
    27481 ccaactgttt taagtaggtt tttatatgac aaactgagtt ctttcaattc acgtatcggc
    27541 cggatgaatc gttcattata gcctaatttt cggattggag tacgagctac gcgactaacc
    27601 tcgtccacta tgaaagggtt ttcaaaccgt tctataatga ctttgtgata attctccaat
    27661 tcttttttat caaagttcca tttggcaatc aagagactcc gaatttcagc taatacagat
    27721 tcaatccgag atttgatatt aggattttga agagcttcca aaattgtctt ggcaccataa
    27781 tgcgcaccaa tgtaagctga agttgcatgt ccagaattga ctgaaaaaag ttttcgctca
    27841 ataaagggtt ctaaatcttc ttcataatgc acatctttta gacgtaaatc tggattttta
    27901 agacgcttgg tttccacgac ccattcatta aagggctcga ccacaacaaa aagggaatct
    27961 tcgtgacttt gtgctggaac aatcctgtct actgcagcat ttggaaaacc tatgtagtta
    28021 tcagcaaatg tcaaaccttc cggacttaaa tatttcttga cttcttgata aagaaattga
    28081 gacccgccaa tcatattttc acaggccata acatccaata cctgtgtatt ccctgcaact
    28141 cggcgagctt cgattccttt ggctagaagt tcagcgataa aagggagtat attaggtccg
    28201 attgcagtag taataatatc cgtcttttga atcgcttcaa tgacttgctc aggatgttct
    28261 ttgctattaa tgccagccac attagttact tctatacgag actgtccttt ctgtgcaatt
    28321 tcaatttcat acttgccctt ttcatttaga gcatgaatta tctgattatt gacatccaca
    28381 aaatcaatat ggaaaccatt tttaaataga atttcaccta taaaaccacg accgatatta
    28441 ccggcaccaa aatgaacaga atgtttcatt caatttctcc ttttcctatt tgagggacat
    28501 atatactaga tgacaaatga cgaaattcaa ttattcaaca gcatcaaata agcgcaatac
    28561 ttcctctttt gactgagcat ctgctagttt aagaacatta tccacatctg cacagaagat
    28621 agaaattttc tgaataattt ctaagtgttc attaccaata ccagcaatac caaaaagaac
    28681 cgttgccact tgagggttag atacattccc aaaatcaacc cctctaggga cttgaacgac
    28741 tgtaatacca gacttaagga catcattttt tgctgcatct gttccatgtg gtatggcgat
    28801 aaagttaccc atgtaaacag atagctcttt atctcgctca atcatggctt caatatagtc
    28861 ttcattaaca tatccaccct cataaagaac ttgcccacaa taacaaatag cttcctcctt
    28921 gttagaaaat tgtttattaa gcttaatcaa atgtttttca agtttcatat tagttctcca
    28981 atttcttaat tttttcgtta aaaatagtat ttagcatctg ataaataatg gattgatttc
    29041 ccgtcttgta aatctctgta taaagatgat tttcaataat cgactgacta atagctgtca
    29101 ttaaatctct agcttcctcg ctagcttctt tagacattag cattagcaga caccttttga
    29161 cctcttcttc ctctcgtttc attgacaatg cggagatagg tagtttgagt tcaaacatag
    29221 taaaactatt tgttgttact ttgctagact gactatgtaa aagcaccaga ccagtattag
    29281 gaatagccat aggactattc tggaagtgag ataacaattt tcttgttagg taatctctgt
    29341 cactcacatt cttctgatat tggatgattt gttctaccgt gtgctcaaat gattgattat
    29401 tctccaattg aaccaactcg aattgactca aaagctgact actagatatt aaataatctt
    29461 gcaagtcata acttttctct ggagcgatag catcacgcgc gacaattgta cgatgtgcct
    29521 gtacatactg aagtcgttcc tgtaattcaa acaattcttt gacggttgga aaactagaaa
    29581 ttacatcgat ttcctgatta gtcagcggct tggtagataa aatataatca tactgactga
    29641 gatcaatact gtggtaatct actagagact gaatatctat caactctaca aaaggagcaa
    29701 cactcttaat tttactgact aataaatctc tagtgacccg acgttcatcc gttaaaagca
    29761 aaacacggac tggataaatc tctggactac ggcctaagct agagataaaa tgtagggcaa
    29821 tcatgccata ctcatactct gtatgaagat atttcggaaa aatatcattc actaaaagac
    29881 tgatgactgt ataaagaaat ttattccttt caactaaaat ctggacagat tctggcaaat
    29941 tttcaccctg aaaaaggata gggacgccta aactgagccg aatatgatgg aaaagaaaat
    30001 tgaataaaac cttgtcctta aaaaagtcaa tcttggtata catggaaacc gtatcaatca
    30061 gatttgaaat attgtagaaa aattcaccat caaatttctc cgtaaagagc ggattgtcct
    30121 gacgtttaat gattaattca tccaagatgc tcgcaaaata gataatttcc tgaatactat
    30181 aaaattgtgc agtctgctta gagtaagctt gaaaaatttt ttgagaaatt tccaaagcct
    30241 gcttactagt attaggtgaa ttttcaatgt tttgctcctg acctataaga gataacaaga
    30301 tcgcaaaaaa catcttcatc ctagcatcca tatctggaaa acctgacagt tgcttattaa
    30361 caatctgact ggccagccta gttctatctg cttctaaaat atcaaagctc ccaaaattac
    30421 cggttgaaaa atctgctact gagatacagt ttgtcagtaa aatagccaaa aatcttctct
    30481 taccaactga atcaccagaa atccgataac ctttttgtcg ttcaattttc agatcaaagt
    30541 ctaaaagacg cttatcaata tctgaaatat cctgaataat agttacatta ctgactttcg
    30601 tgcactcttg caatgcttca ttggtgacaa aaccactctc agtcagaagg cgataggtaa
    30661 tcaactcttg tctttcttgg ggactatact caactaacac ttcaagttct gtcggcaaat
    30721 catccaactc tccagtcaaa atatagtatt tcccatgctt cgttatttcg attccatatt
    30781 gctccatgct atctgtcaaa tctgataaag ttcgataaat tgttctagat gaaacctgta
    30841 agatttcagt catatcttgc attgaaagct tccctacatg taggaaagcc ttcaataatt
    30901 gttcttctcg tttggttaat agcattcgta atttagaaac ctctagttct atttgtacat
    30961 tctagcagcc attttgtcat attctggtgt tgttactaaa ctatccacaa taagaaattg
    31021 agaattcggt gctgcttgct gcacttctgc ctgtaaagaa atagttgtta caatcattat
    31081 gtttttagaa ttaaattcac ctaattctga aattttggca gtagaaactg gaatacgaat
    31141 attcttgttt ctaaaaatag cccgaatcgt ttcacagccc atagttgcag ttccctgtgc
    31201 tttaccataa gcaactacta cagcatcaat atggttaagg tcaatttcct gactatctac
    31261 tggtgctgaa gttggtatat ctccttcaat ttctgctatt ggagaagctc ctgttaatga
    31321 agctacaatt tcatcataac gaggggacgc taagaaatta tcgacagaaa catgaatagc
    31381 acttggactc ttgtctttag ctcttggtgt cagttcttcc tgagtaacaa ttaatgtttt
    31441 tggtgtatca agcaaatttg agattgcctg attagatact ggaatctcta gacctgcttt
    31501 tttaacctta tctcgaagaa tactagctcc catagcagag cttcccatac cagcatcgca
    31561 ggcgaaaatg attttttcca ctgagtctgt caaaacaact gtatcaacag aagttgatac
    31621 taactgacct ttagactgag ccttagctgc ttgggtagcc gcctgagcag cttcgagcga
    31681 atcctcagtt gacttgtctg catgaagaat aagggctgct acaaggaaag aaacaactgc
    31741 tgctactaaa acacctagaa gaacatttat atggggccaa acaccttttg gcgccgtagc
    31801 cataatcgca ataatagaac ctggtgaagc tggagattta agaccagcgt ctaagagttg
    31861 aaaagtaaaa gttccagaga tacctcccgc catagcagct aaaaatagag taggcttcat
    31921 cataacataa ggaaagtaaa tttcatgaat ccctccgaag aaatgaataa ccattgcccc
    31981 ccaagaagaa gatttagcag aacctttacc gaatacagca taagctaata gaattcccag
    32041 gcctggtcca ggattagctt ccaataggaa gagaattgac ttaccagctt gagctacctg
    32101 ttctactccc agaggagtaa aaatgccatg attgagggca ttattgagga aaaggacttt
    32161 agccggttcg atgataatat tagccatagg aaggaggcga gcattgacaa tagcctcaac
    32221 accattccca acagctccag taagagtcga tacgactgga ccgattgcgt agaaagccaa
    32281 aagcaataat gcaaaaccaa cgagaccagc tgagaagtta ttaactaaca tttcaaatcc
    32341 gggacgaatt ttttcttgga acttctcatc aaatttcttg atagtccatc ctcccagtgg
    32401 gcccattacc atagctccga taaacatagg aacactagaa cctgtgattg caccaacagt
    32461 agcaatagct cctacaacgg caccacgttg gccatggatc atatatccac ctgtgtaacc
    32521 aatcaggatt ggcaataaat acgttaacat aggaccaaca acagtagcta actgttcatt
    32581 tggcagatag ccatcagcga taaagagggc agtcaatact ccccaagcaa taaatgctcc
    32641 aatattgggc ataaccatat ttgaaagcga tgtccctagt ttttgaaccc tgactttcaa
    32701 tgatactttt tcttccatta gattatcctc cttttcttaa aactagtata tacaaagtta
    32761 aaaaccttta aaactaaagt tttgtcacag attttgtgtc aaaacattac ttttgacgag
    32821 gctgggcaaa aaggcttaaa aattataaaa cgcatcatat caggtgtaca tgaactac
//
LOCUS       DAVKQL010000023        31479 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVKQL010000023 DAVKQL010000000
VERSION     DAVKQL010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31479
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.023"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(1..275)
                     /locus_tag="VW561_000965"
                     /pseudo
     CDS             complement(1..275)
                     /locus_tag="VW561_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(288..1061)
                     /locus_tag="VW561_000966"
                     /pseudo
     CDS             complement(288..1061)
                     /locus_tag="VW561_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566051.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(1507..2262)
                     /locus_tag="VW561_000967"
     CDS             complement(1507..2262)
                     /locus_tag="VW561_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEU9943247.1"
                     /translation="MIDVEEILCKMPPNQKINYDRVMQKMVQAWEKNEQRPTILVHVC
                     CAPCSTYTLEYLTKYADVTIYFANSNIHPKVEYHKRVYVIKKFVSDFNERTGNTVQYL
                     EAPYEPNEYRKLVRGLEEEPEGGDRCKVCFDYRLDKTAQVAMDLGFDYFGSALTISPH
                     KNSQTINSIGIDVQKIYTTHYLPNDFKKNQGYKRSVEMCEEYDIYRQCYCGCVYAAQA
                     QNIDLVQVKKDATAFLLDKDVEKDYSHIKFIVD"
     gene            complement(2400..4022)
                     /gene="groL"
                     /locus_tag="VW561_000968"
     CDS             complement(2400..4022)
                     /gene="groL"
                     /locus_tag="VW561_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU9943248.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(4038..4322)
                     /gene="groES"
                     /locus_tag="VW561_000969"
     CDS             complement(4038..4322)
                     /gene="groES"
                     /locus_tag="VW561_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEU9943249.1"
                     /translation="MLKPLGDRVVLKIEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(4592..4987)
                     /locus_tag="VW561_000970"
     CDS             complement(4592..4987)
                     /locus_tag="VW561_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU9943250.1"
                     /translation="MYNKVILIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(5065..5826)
                     /locus_tag="VW561_000971"
     CDS             complement(5065..5826)
                     /locus_tag="VW561_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU9943251.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHVINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            complement(5859..6485)
                     /locus_tag="VW561_000972"
     CDS             complement(5859..6485)
                     /locus_tag="VW561_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEU9943252.1"
                     /translation="MIFTYNKEYVGDVLMVIVKNSGDAKLDVERKGKVARVFLKDNGE
                     TVAWNIFEVSSLFEIAERGQVFLTDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKTVQIVAGAPNARVGLKTIVALPGAMMPKGNLIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            complement(6501..6818)
                     /locus_tag="VW561_000973"
     CDS             complement(6501..6818)
                     /locus_tag="VW561_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU9943253.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKQQINDFLA
                     GLK"
     gene            complement(6815..7114)
                     /locus_tag="VW561_000974"
     CDS             complement(6815..7114)
                     /locus_tag="VW561_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEU9943254.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDLFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFIYENEDLVYEEEVL"
     gene            complement(7399..7590)
                     /locus_tag="VW561_000975"
     CDS             complement(7399..7590)
                     /locus_tag="VW561_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HEU9943255.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            complement(7751..8425)
                     /locus_tag="VW561_000976"
     CDS             complement(7751..8425)
                     /locus_tag="VW561_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943256.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLTIRGDVAFGKL
                     EIVYVK"
     gene            complement(8431..8877)
                     /locus_tag="VW561_000977"
     CDS             complement(8431..8877)
                     /locus_tag="VW561_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9943257.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(8991..9494)
                     /locus_tag="VW561_000978"
     CDS             complement(8991..9494)
                     /locus_tag="VW561_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU9943258.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(9491..9853)
                     /locus_tag="VW561_000979"
     CDS             complement(9491..9853)
                     /locus_tag="VW561_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 family protein"
                     /protein_id="HEU9943259.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICSVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(9919..11778)
                     /locus_tag="VW561_000980"
                     /pseudo
     CDS             complement(9919..11778)
                     /locus_tag="VW561_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11817..12266)
                     /locus_tag="VW561_000981"
     CDS             complement(11817..12266)
                     /locus_tag="VW561_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9943260.1"
                     /translation="MDKPMLVFKRFGHQIHLMVQKEAKRCGIEFMGGPQGQVVHFLDN
                     REKNQDLVLIKDIEQELNITKSVASNLVKRIVQNGLVELEASPVDKRAKFVRLTDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVIGQLQAKYQGNRRRE"
     gene            complement(12390..13106)
                     /locus_tag="VW561_000982"
     CDS             complement(12390..13106)
                     /locus_tag="VW561_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9943261.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(13987..15402)
                     /gene="ply"
                     /locus_tag="VW561_000983"
     CDS             complement(13987..15402)
                     /gene="ply"
                     /locus_tag="VW561_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEU9943262.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(15413..15745)
                     /locus_tag="VW561_000984"
     CDS             complement(15413..15745)
                     /locus_tag="VW561_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943263.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDHLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(15816..16424)
                     /locus_tag="VW561_000985"
     CDS             complement(15816..16424)
                     /locus_tag="VW561_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943264.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(16437..16895)
                     /locus_tag="VW561_000986"
     CDS             complement(16437..16895)
                     /locus_tag="VW561_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEU9943265.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            17020..17416
                     /locus_tag="VW561_000987"
                     /pseudo
     CDS             17020..17416
                     /locus_tag="VW561_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(18157..18414)
                     /locus_tag="VW561_000988"
     CDS             complement(18157..18414)
                     /locus_tag="VW561_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943266.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDITRLTASLLGDEKLSAITSSSV
                     KADIHRFVIELEPVKSTILQNDDISLDQNEIFEILKNFLDG"
     gene            complement(18473..19024)
                     /locus_tag="VW561_000989"
     CDS             complement(18473..19024)
                     /locus_tag="VW561_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943267.1"
                     /translation="MPVNTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIYEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFPSMIELFSIL
                     PEYPLKKDGREIPLHFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCGLYSSKI
                     SSNHVSFHLQPQNSVLSSLQLAS"
     gene            complement(19014..19799)
                     /locus_tag="VW561_000990"
     CDS             complement(19014..19799)
                     /locus_tag="VW561_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943268.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEIPKELTPSEQLTWIAFLLPDGDIKQIKSVSNLLYGGAYALS
                     HSTFLAETDENTSYVIWQRKFNQLSLPLSQHVLKGKMLEIWKYRPFVSEFWNDFKNNH
                     DKQFVDPISLYLTLKDDDDPRIEEESEALENMILQYLGEDDAS"
     gene            complement(20207..20632)
                     /locus_tag="VW561_000991"
     CDS             complement(20207..20632)
                     /locus_tag="VW561_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943269.1"
                     /translation="MTSRVFIDADCISAFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVTKGSAEIVSIDIGTEEYALYRDLTRNYDSNKIIGKGEATSIFLA
                     KKHNGILGSNNLRDVKPYVEEFSLEHMTTGDILIEAFKA"
     gene            complement(20625..21119)
                     /locus_tag="VW561_000992"
     CDS             complement(20625..21119)
                     /locus_tag="VW561_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943270.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKKEMVLGHYIKSTEQLLENNRISQGKYEELLLDAFRYDIVYGLDEEGE
                     LSFD"
     gene            complement(21165..21371)
                     /locus_tag="VW561_000993"
     CDS             complement(21165..21371)
                     /locus_tag="VW561_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943271.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMPLGR"
     gene            complement(21358..21519)
                     /locus_tag="VW561_000994"
     CDS             complement(21358..21519)
                     /locus_tag="VW561_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9943272.1"
                     /translation="MNMINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            complement(21884..22840)
                     /gene="lytA"
                     /locus_tag="VW561_000995"
     CDS             complement(21884..22840)
                     /gene="lytA"
                     /locus_tag="VW561_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEU9943273.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(23216..24586)
                     /locus_tag="VW561_000996"
     CDS             complement(23216..24586)
                     /locus_tag="VW561_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU9943274.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQSSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKQPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSTFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            complement(24893..26059)
                     /gene="recA"
                     /locus_tag="VW561_000997"
     CDS             complement(24893..26059)
                     /gene="recA"
                     /locus_tag="VW561_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEU9943275.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVTLDLGDELEIEIEE"
     gene            complement(26114..27370)
                     /locus_tag="VW561_000998"
     CDS             complement(26114..27370)
                     /locus_tag="VW561_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEU9943276.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGASSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQEQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            complement(27455..28471)
                     /locus_tag="VW561_000999"
     CDS             complement(27455..28471)
                     /locus_tag="VW561_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU9943277.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            complement(28479..28997)
                     /locus_tag="VW561_001000"
     CDS             complement(28479..28997)
                     /locus_tag="VW561_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9943278.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQAGGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            complement(28987..29430)
                     /gene="tsaE"
                     /locus_tag="VW561_001001"
     CDS             complement(28987..29430)
                     /gene="tsaE"
                     /locus_tag="VW561_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU9943279.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDTYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            complement(29516..30136)
                     /locus_tag="VW561_001002"
     CDS             complement(29516..30136)
                     /locus_tag="VW561_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943280.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTVFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            complement(30519..31382)
                     /locus_tag="VW561_001003"
     CDS             complement(30519..31382)
                     /locus_tag="VW561_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9943281.1"
                     /translation="MNTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEVKSNLSNFKHLSARLLDDRNYDDLEYLYKIEVERS
                     TFLTLEDRTYLEWIKAIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
BASE COUNT         9474 a         6926 c         5340 g         9739 t
ORIGIN      
        1 aatcctttaa tattcaaact ataaaaattt ggagataaat taaccaaaaa atcatcacgc
       61 tccgatcttt tataatgttc taggtgatta caaaaattct ctatcgaaac attgatattt
      121 gataataatt caaaaaatgt tgaaagagaa atttcatttt ctcccctttc aaatctagaa
      181 agttgagatt ttgatataga atcagctgca gcatactcta aagaatatcc gtttaatttt
      241 ctaaaatatc gaaaagcttc tccgtaattt ttaacacttt cattccctta actttctttt
      301 cgagcgataa acataccata ttctttagga atgatcaaca taccattatt aaactcactt
      361 tccaatcttt tacgtattat ttcctcttct agtgaaccta tttctgaaat tcctttaaac
      421 gattggatgt atttaactag gtcatctata ctagttacct gtaactcatc atcatagagt
      481 aatgtatcga cagagttaaa acacctactc aggtaccttt taccattttg taaagtaaac
      541 gttttatttt ctaaatctta attaacctca tctttaaata aatttgccaa ataatttaca
      601 actccatttt caccaaatgt agcacagtaa aaaatgcctt cggtttttaa gactctattc
      661 acctcagaga gagctttagg aatatcattc acatgatgta gaagcatatt agcaataaca
      721 atatcaaaag tttcattctc aaaagaaatc ttttgaatat ccattatctc atagttgaca
      781 ttgtctctat ttccaatcac tgacttcgtt gattttacca tatctttaga aaaatctgta
      841 acaatcaact gcttcatttt atctatagaa tcactattac ttttccacag ttctcctgta
      901 ccacatccca attctagaac ctttacttca tcagtgattt ggtagttaga aaatatccaa
      961 ttattaaatc ccaatttatt ttttgaatac ttcttatgca attctactcg aatatcaaga
     1021 ttatcatgac ttttatattg ttcaattact ttatcaatca ttttaaatga cctccactct
     1081 ataatttggc attatatcac aagaataaag atagtgttaa ataagttgta taaatgcaaa
     1141 aaaggaatat tccgttatag cagagttgta acacatttct ataaagaaat ttattcctac
     1201 gactcagttt ggcatgctta acttcctaac tcaaaaacaa agcatcgatt aatttatccg
     1261 ttcttctttt gaaacagatt tgaactgata attgctcgtt ttaatttaag aagctctttt
     1321 gagcaaaact tcattttttt aagagtccaa caatttctgt ctatcgcttt tctactagca
     1381 aaaaacctac cttgaggcaa gttttcattt atatatctaa tttcgtaata gtatagtaaa
     1441 atgaaataag gacagtcaaa tcgatttcta acaatgtttt agaagtaaag gtgtactatt
     1501 ctagtttcaa tctactataa atttgatatg agaatagtct ttctcaacat ccttatccaa
     1561 caagaatgct gtggcgtcct tcttaacttg aaccaggtca atattctggg cttgggctgc
     1621 atagacgcag ccacaataac attgacgata gatatcatac tcctcacaca tctctactga
     1681 acgtttgtag ccttgatttt tcttgaaatc gttgggaaga tagtgggtcg tgtaaatttt
     1741 ttgcacatcg attccgatgc tattgatggt ttgagaattc ttatgaggac tgatggtcaa
     1801 ggctgaacca aagtagtcaa agcccaagtc catagccact tgcgctgttt tatccagtcg
     1861 gtagtcaaaa caaaccttgc aacggtcgcc accttcgggc tcctcctcta gtcccctaac
     1921 tagttttcgg tattcattgg gttcgtaggg agcttctagg tactgaaccg tatttcctgt
     1981 ccgctcatta aaatcactaa caaatttctt gatgacatac acccgcttat ggtattctac
     2041 cttgggatgg atattagaat tggcaaaata gatggtcaca tctgcatact tggtcaaata
     2101 ttctagtgta taggtactac aaggggcaca gcaaacatgc acgagaatgg ttggccgctg
     2161 ctcatttttc tcccatgctt gtaccatttt ctgcatgaca cgatcataat taatcttctg
     2221 attggggggc atcttgcaca gaatttcttc tacatcgatc atgttcttct cctttttcta
     2281 gtcttatttt atcatataag ttaggagagc tcaaatcaat ttagaagtga atattataaa
     2341 aagaagggtt agtcattccc tccttttgtg ttttttataa gttgttttct atagaaagct
     2401 tacatcatcc cgcccatcat acttggatcc attgctggag ctggggctac tggttctggt
     2461 ttattggcta cgactgcttc tgttgtcaaa atcaagcttg ctacagatgc tgcattttgt
     2521 agggctgaac gactcacttt aactggatca atgatacctt gatcaatcat gttaacccac
     2581 tcgccagttg ctgcgttgaa tcctatacca agctcagcat ttttcaaacg atcgataacg
     2641 atagatcctt caaatcctgc attgtgagca atttgacgaa cgggttcttc caaagcacgg
     2701 agaacaatat tacgtcctgt tgcttcatct cctgtcaatt ccaaggtagc aacagctgga
     2761 atcacattgg caagagctgt tccaccacct gcaacaatac cttcttcaac agctgcacga
     2821 gtagcgttga gggcatcttc aatgcggagt ttcatttctt tcaactcagt ttcagttgcg
     2881 gctccgacct taataaccgc tacaccacct gacaatttgg ccaagcgttc ttgcaatttt
     2941 tcacggtcaa attcagaagt tgtagtttcg atttgagact tgataaccgc aacacggtga
     3001 gaaatcgctt caggatttcc tgcaccttct acaataaccg tgctatcttt gtccacggtc
     3061 actctcgctg cttgaccaag agcttcaatt gtcgcatctt tcaactcaag accaaggtct
     3121 tctgtgataa ctgttccgcc tgttaagatg gcgatatctt caagcatggc tttgcgacgg
     3181 tcaccaaaac caggtgcctt gactgctact acgttgaagg ttccacgaat cttgttcaaa
     3241 acaagagttg gaagagcctc gccatccaca tcatccgcaa taatcaagag tggacgattg
     3301 ctttggagaa tgctttccaa aagtggcaag atttcttgga tattggaaat tttcttgtct
     3361 gtaatcaaaa tgtacggatt ttcaaggtca gccaccattt tttcgctatc agtcaccatg
     3421 tactgtgaaa ggtaaccacg gtcaaactgc attccttcta cgacttcaag ctctgtttcc
     3481 ataccacgtg actcttcgat ggtgatgaca ccgtctttgc caactttttc cattgcttca
     3541 gagatgtact caccaacttt ttcagaacga gaagatacgg ctgcaacttg agcgatagct
     3601 tctttattgg caacagggat ggcgttgttt ttcaaagctt caactgctgc ggcaactgcc
     3661 gtttcaatcc cacgacgaat accgattgga tttgcacctg ctgtgacgtt tttgattcct
     3721 tcacggacga ttgcttgggt caaaacagtt gcagttgtag ttccatcacc tgcgatatca
     3781 ttggtttttg aagctacttc tgataccaat ttggcaccca tattttcaaa atggtcttcc
     3841 aattcgattt ctttggcaat ggtcacaccg tcattggtaa tcaaaggtga accgaatgac
     3901 ttttcaagaa cgacattgcg accttttggt cccaaggtta ctttaacagt gtctgcaagg
     3961 atatcgacac cacgaaccat ggctgaacgg gcatctgatg aaaatttaat ttcttttgac
     4021 atacttactt tctccttcta ttcctcaatg attgccaaaa tgttagcttc gcctacgatg
     4081 atgtactttt catcgccatc tttgacatca agacctgcgt gggcttcaac taagacacga
     4141 tctccagttt taacacttgg agcaaccaag tcaccgttca aggtacgaac accttgtcca
     4201 gtagccacaa cttgagctgt tttggttttt tcttgggctg agcctgcaag gacaaagccc
     4261 ccaacggttt gttctttttc ttctattttt aagaccacac ggtcccctaa tggtttcaac
     4321 atctgatttc ctccataatg agatagatat tattagcact ctttgaactt gagtgctaat
     4381 tcataattct attgtatcac ttggtcagaa atagtcaaga aaaaagtctg actttctcaa
     4441 gataaaaagc ctgagaccaa ctcagacttt ttaatgttta tactcaatga aaatcaaaga
     4501 gcaaactagg aagctagccg caggctgctc aaagcattgc tttgatgttg tggataaagc
     4561 taacgtggtt tgaagagatt ttcgaagagt attaaaatgg caattcttcc tcttccaaga
     4621 ccaaatctgc caaatcttgg cctgcattat tttcacgcat ggcacgttga gcacgacttt
     4681 ctaagagttg gaatcctgtc acaaggactt cggttacata gttcatctga ccatttttct
     4741 caaagcgacg ggtacgcaat tctccatcaa cggaaatgag actacctttg gttgcgtagc
     4801 ttgccaaagt ttctgctagc ctgccccata ggaccatatt gacaaaatca gcttcacgtt
     4861 caccgttttg gtctttgtaa cgacggttga cagcgatagt tgctcgcgct accgacttgt
     4921 cattgttggt tttgtgcaat tctggtgtag acgttaaacg cccaatcaag ataactttat
     4981 tatacatatt ttcttcctcc tacttatcta ttcgtaggaa atcaaaaaaa gttacagaaa
     5041 tttgtaactt ttcgaggaaa tttcttattt tttatgaatc atgaaacctg tcgcctgttg
     5101 attggccata atggtcatat ctgtaatctg aacacggcga ggctgactgg tcacatagac
     5161 tactgtgtct gcaatatcct gagcttgcaa ggcttctatt ccttggtaaa cggacgcagc
     5221 ccgctcttta tcaccatgaa aacgaacagt tgagaaatct gtttcgacaa tcccaggctg
     5281 aatggttgtc accttgatat ccgttgcgat ggtatcaatt cgcagtccat ccgaaaaggt
     5341 cttaaccgct gccttggtag ctgagtaaac agcggcacca gcataggcgt agattcctgc
     5401 ggttgacccc atattgataa cgtgaccttg attgactttt atcattgctg gcaagaaaca
     5461 gcgagtaacc gccatcagac ctttaacatt ggtatccaac atggtcaaca tatccaactc
     5521 ctcatagtct tgatagggag ccaagtcaag agccagtccg gcgttgttga ccaagatatc
     5581 aatcctccct atcgtttcta gaatatcaga gcaaacagtc ttcaccatga ccatatccgt
     5641 cacatctagt ggaaaagtcc agacagtttg atttggaaag cttactgcaa actccgactt
     5701 gaggatttct aatctgtcta tccgtcgtcc tgttagaacg acatcctcac cctgctccag
     5761 ataagcacgc gcaatcgctt caccgattcc tgaggttgct cccgtaatca ctacattttt
     5821 tgccatctta tttccttcta tcagatgctg acaagttcct aggcagtcca gtgtttcgct
     5881 gggtcgaacg gtgttccgac aacttggtct tctgataatt caagcacccc acgtttttgc
     5941 ggagcatttg gcagatgcaa ttcacgagga ctgcacatca taccaaaact cttttcacca
     6001 cgaagttcac ctgggaaaat gagattccct tttggcatca tagctccagg aagcgcgaca
     6061 atggttttca accccacacg cgcattggga gctcctgcaa cgatttgtac agtcttatca
     6121 cttgcgactg caacttggca gatgttgagg tggtcactat ctggatgggc taccatctca
     6181 acaatttcac cgacaacaaa cttaggttcc ttatcattaa caatttcttc tgtaaaacct
     6241 tccgcctgca actcttggtt caaacgagcg acttgctcat ctgtcaaaaa gacttgaccg
     6301 cgctctgcaa tttcaaataa acttgaaact tcgaaaatat tccaagccac tgtttcccca
     6361 ttatctttga gaaaaacacg ggctaccttg cctttgcgct ccacatccag tttggcatct
     6421 ccgctatttt tcacgatgac cataaggaca tcaccgacat attctttgtt atacgtaaaa
     6481 atcattgttt cctttttctc ctatttcaat cctgctaaaa aatcattgat ttgttgcttg
     6541 cttttacggt cgcgattgac aaaacgaccg atttccttgt ccttttctag aacaacaagg
     6601 ctaggaattc cgtaaacatc ccagagtttg gccaaatcca tatactgatc tcggtccatt
     6661 cgaataaagg tgaactctgg attggtctcc tcaatctctg gtaaggcagg ataaatataa
     6721 cgacaatcgc cacaccagtc tgccacaaaa aggaagacct tcttgcccgc tttttccact
     6781 aaagatgcta attcttctaa acttgctggc tgtatcataa gacttcctcc tcatagacta
     6841 ggtcttcatt ttcatagata aaggtataat gacggccatc ctcaaaaatg acgccaccaa
     6901 ccaagctctc cagactgctt tcgtaaactt gaacatagag ggtcgcaatt tcccccatgt
     6961 cggaaaaaag gtctcgcaca atctctgtca actcttcctg agtcttcatg agcttacggt
     7021 catctgcaac ttttttcgta gcaagagcaa ggcttccgat acctagcaga gccaagcctg
     7081 ccatccacat ttttttagct ttcataccat tcattttaac acaaaaaagg cttcaggaca
     7141 aatgaggaag cagcagaaaa gcaagtaaaa agcctcttcc tttaaggaaa aagacttctt
     7201 atactaagag caaactagga agctagccgc aggctgctca aaacacggtt ttgaggttgt
     7261 agataaggcg acgttgacgt ggtttgaaga gattttcaaa gagtattatt cttattgcca
     7321 ggcacctaag ttgccaacgt agtaactatc aggtgtgtag gtattgcgaa gcatcttacc
     7381 tgatgaagcc agataatact acttgccatt gtctttgacc caatcattcg caatcatgga
     7441 accagaagaa cttacataat accattctcc cttgtcataa acccaagtac tgactttcat
     7501 ggttcctgag caattaaagg caaaaaaact gtccaatatc attcgttttt taaaagcatt
     7561 tgacactaca tcatctacgc tgtagtacat agatacatca aacgtaaaag ccaaaatatc
     7621 gaaaaatata caacttttaa aggcagcatt tcatttttta aagtctaaca accatagaac
     7681 tgaaatcaga gcaaggcatc aatttcctac tcttagtacg tctattttga tggttgaagt
     7741 gaagattttt ttatttaacg tagacaattt ccaacttccc aaaagccaca tctccacgga
     7801 tagtcaaggt tttgctggtt ggggctacag gagcgtcagc cttagctgca ccaaaggagg
     7861 tttctacttt caaatctaca cgccagtgtt gtggaacata gacggttgca tttccgaagg
     7921 ccacttcaat atttaaagtt gcaaaatcac ctagcatctc tgcattatca tagtagattt
     7981 tagcatcccc aaaagcgact tccacttggt catctacgag atcttgatct tgcttataga
     8041 aggtcccgct accaaaagcg acttccttat ccgtgacgac tgtttttttc ccattgtacc
     8101 accatttttt ttcattccag aacttacttg aatgcgtcag ataaccaaca ccaagtacca
     8161 ccaagatgct agcccaaata agagaatgat tggtaactgg taacaagtca taagcgtaat
     8221 ttgcaatgat gagcgccagt aaacctgtaa aaactgccga agtgagatga cgtctaagga
     8281 tggactcaat ggacttataa gcaaaaaaaa caatacctag taaaggccat attttaccat
     8341 ccaaagaagg aattccaaaa ttcccttgca gcaagatcca agctgctaaa accaacaaaa
     8401 caataccaaa tgctttcttt ttcatgtttt ttacctcatg tttcttagat tttcttttag
     8461 gagggattgg taatgccgtg agacatgaac ctccttgtgc gtctgataaa aggaaatggt
     8521 gcccgttcct gaaaaggact tgtccactga gtaaatctga cggatgtttg cgatcgttga
     8581 cttggaaact cgattaaaat agcgaggcaa gatagactcc aactcataga gcttgaggcg
     8641 aacctcataa gcttccttct ggttatgagc gtagatcttg ctcccttctg tttcaaagaa
     8701 gagaatttct gacaggtcta gataatattc acctgtcccc ttgtaaaaag tcaacctcgg
     8761 agcctttgac tcttgcaaga gccgttgcaa gtctgcaatt tcatctgtca aagtgggggt
     8821 cttgatgaca atttcagtct cctctaaatt gccgtcaatc tcaattcgta acttcatcac
     8881 atctcctttc tgactctact atatcaaagc taagttagga atacaagggc aaaaaagcaa
     8941 gtggtcaatt tgaagcagta agtggttgta ggactagctt aacttacatg tcagaatagg
     9001 aagaaaagga atccccacac caaaccacaa gcatagccaa ccaagacatc cttgggataa
     9061 tgaacacccc ctaaaaccct caccaacccc agcaaagctg aaaagatcaa gcatactaag
     9121 ccaataggta gactagcatg cagacaggcc atggagatga tagttgccga aaagacatgg
     9181 cgactgggca tcgaccgtcc cgaactatcc ttatcaagaa ggggacagat atcccaagtt
     9241 tcataaggtc tcgggtgatt gattttctta cgaaacaggg acaaggccac aaaacctgat
     9301 gcaggaataa gcacataaac cccaacttgc tgaccaagtc ctagttgcag gtaagtggtg
     9361 actagcaaga tcagatagac caggggcatg gcgactgtca tcaaacgatt gaaactgcgt
     9421 aataggaata aaagaattgg acgactagtt agtctggagc tgatatttcg ataccattct
     9481 tgataatttt tcatttatgc ttctcatact tttctagaac ttgttgagat gttttcccct
     9541 tggcaagcca atccactagc ttgtccaagc gacgtatttc ctgcatcaga ggatcatcaa
     9601 tttcctcaat acggacactg caaatcttcc ctgtaattgc aattcgttca gggacataat
     9661 atggtgcttg tctaaaaaaa tctccgtaag taacatccct atccagacaa gcaagaacat
     9721 cactcacttc ataccctgtc aaccaactgg tcacttggtg aactgactct gcctttccac
     9781 cctttcgctc aacttttgca atcaaggcta agtagacagc agcaaatttc atattgtata
     9841 atttctgact catacttctc tctttctatg tgtactagta taaataagaa aaagaaggcc
     9901 gtcaagcctt cttttgattt attcttctgc ttcatcttct gtaaattgac tattgtacaa
     9961 gtcagcgtag aagccacctt gcgccatcag ttcctcatag ttgccttgct cgatgatatt
    10021 tccatctttc atgaccaaga tcaagtctgc atttcggatg gttgacaagc ggtgggcaat
    10081 gacaaaggat gtgcgtcctt ccatcaaacg gtccatggct ttttggatca attcctctgt
    10141 ccgtgtgtca acagaagaag tcgcctcatc caaaatcaaa agcggtgcat ccttaagaag
    10201 ggcacgagca atagtcaata gttgtttttg tcctacagac aaggtcacgg tgtcatccaa
    10261 gatggtatca tagccatctg gcaaggtcat aataaagtgg tgaattccca cagccttact
    10321 agcttccatc attcgttcat cactaatccc tatttgatta tagatgagat tgtctcgaat
    10381 agttccttca aagagccagg tatcctgcaa gaccattgaa aaggcatcgt gcacttctga
    10441 acgcgtcata gccttggtat ccacaccatc aatgtgaata cttcccttat caatctcata
    10501 gaatttcatc aaaagattga caatggttgt cttaccagtc ccagtcgacc caacaatggc
    10561 aaccttttga ccagcatgag ctgtcgcaga gaagtcatag tcttgaacat tgacaccgtc
    10621 caccagaatt tctcctgctg acacgtcgta gaaacgtgga atcagattga ccagagttga
    10681 tttaccagaa cctgttgacc caataaaggc cactgtttga ccagtttctg ctttaaagct
    10741 aacatgttca ataactgcct ccgaatttgc cgcatagcgg aaggtcacat ccttaaactc
    10801 gacctgacct ttgaagtttt catcagtcag ctgtacttga acagggtttt ggatagaaga
    10861 atgcaaatct aaaacttgat taatccgctt agcagagacc atagttcggg gaagaacgat
    10921 gaagagtgct cccatgagaa ggaagcccat gacaacctac atagcataag acatgaaaac
    10981 aatcatgtca ctaaagagag gcagacgcgc tatcggagca gcgtcgttaa tcacataggc
    11041 cccaatccag taaatcgcca cactcaaacc acttgaaatc cccatcatga taggattcaa
    11101 aatagccata agacggttga caaacaaatt ttaacgggtc aattcatcat ttactgctgc
    11161 aaatttttca ttttgataat cctctgcatt gtaggcacga acgacacgaa tacctgttaa
    11221 actctcacga gtgatactgt tcagtttatc tgtcagcccc tgaatcaagg actgttttgg
    11281 aaaggctaac gtcatcaaaa cggtcgtcat caggacgttg ataatcactg ccacaagtac
    11341 ggcccagagc cagtattctg aatgacctaa aatcttccca atagcccaga tagccataat
    11401 tggaccacac gttaccactt gcaagcccat agtaatcaac atttgaactt gagtaatgtc
    11461 attggtagta cgcgtcaaga ggctaggaat tgaaaatttc ttaatctctg tctgcgagta
    11521 atccaaaact cggttaaaaa tatcacttct cagcctacta gtataagaag ccgccactcg
    11581 ggatgcaaaa aatccaactg caactacgga caagaaggca agaaaggaca ttcccatcat
    11641 catgcttgcc ggctgccaca actcatctaa attagtttct tgactaccta gcaaatccgt
    11701 aattttcgag atataggtcg gcactttcaa ctctagatag accaaaaagc aagtaaagag
    11761 aatggctagt aaaatcatcc cccattcttt tctactaatt cttttggcta atttctttat
    11821 tctctcctcc tattcccttg atattttgcc tgtagttgac cgataacctt ctcaaaaatc
    11881 agtaattcat cttcatcaat gtcttccatc aactgcttgt ctatgcgttc aaaaaaagcc
    11941 ttaacctgtt gcatctgaga acgtgctttg tccgtcagac gaacaaactt agcccgctta
    12001 tcaacaggac tcgcctccaa ttccaccaaa ccattttgca ctatacgctt aaccagatta
    12061 ctagcaacag acttggtaat attgagttcc tgctcgatat ctttaatcaa gaccaagtct
    12121 tggtttttct cgcgattatc caaaaaatgc acaacctgac cttgcggccc acccataaat
    12181 tcaatgccgc aacgtttggc ttccttttgc accatcaggt gaatttgatg accaaaacgc
    12241 ttaaagacta acatcggttt atccataatc tcccccttct aaataaaaat agttctctgg
    12301 agaataatta aatttctatg agaactattt tcttgattaa aaaaatccca agtgattttc
    12361 tcacttagga tcatgttcta taggttaaat taaaacccat ctacgttcgt ataaatcttt
    12421 tggacgtctt cgtcgtcttc aagaacgctg taaagttttt caaaggtttc aaggtcttcg
    12481 cctgacaatt ccacttctga ctgaggaatc atttccaatt cagtcacttg gaattcttca
    12541 ataccagact cacggagggc aacgatagcc ttgtgaaggt cagttggcgc tgtgtaaact
    12601 gtgattgtac cttcttgtgc ttctacgtca tccacatcca catccgcttc gagcaattgc
    12661 tcaaagactg cgtccgcatc ttcacctcca aatacaataa cacctttgtt gtcaaagagg
    12721 taagaaacag aacctgaagc gcccatgttt ccgccgtttt taccaaaggc tgcacggaca
    12781 ttggctgctg tacggttgac gttagaagtc aaagtatcca caattagcat agagccattt
    12841 ggcccaaaac cttcgtaacg tccttctgta aaggtttcgt ctgtgtttcc tttggcttta
    12901 tcaatcgctt tatcaataat gtgttttggc acttgggctt gtttagcacg gtcgataacg
    12961 aatttcaaag ctgagtttga ttctggatct ggatcacctt ttttagctgc tacatagatt
    13021 tctacaccaa attttgcata taccttagag ttagctccat ctttagccgt tttcttggct
    13081 acgatattgg cccatttacg tcccattagg aatctccttt tttcacattt taatctttat
    13141 tattataaca caagtttttt tgattttcac tagaggaaat ggattttatt agcaaatcaa
    13201 gctaggatag cactttacct gctaagatgg tcttaccttt ctatctttat caacagacac
    13261 tcatccacat tcaaaaaaca aactagacca ttatctgcaa atagaaagtt tcagccaagt
    13321 ttgacaaagt cagctcaaat tactgtttga agtttgtaga tataagcgac aaaaacaatc
    13381 atactgcacc ttttgttgac agtctactcc agacatatca tagttcaagt aaatactttg
    13441 aaattcaaca gttcttatag gcgctattgt attctaagaa atcaatagaa gagtttctaa
    13501 gcaaacctct aatactcaat aaaaatcaaa gagcaaacca gaaagctagc cgcaggttgc
    13561 tcaaaacact gttttgaggt tgcggatggg gctgacatgg tttgaagaga ttttcgaaga
    13621 gtataattta cgtgttccca agatggagaa gttagactag tacactggca cttctaaacc
    13681 attgctagca attgatttgt tcatatttaa tttcattttt tccataaatg ggtattagat
    13741 ataaacagca aaatatttcc gatacgtgtc gttcttgaat ttccaatcat ctaaaacaag
    13801 taaaggataa tcaatcccct gtatatcaag gaattggcta ccctttttac ttttttacac
    13861 attctgtttg atagattcat tttaacatca cgagcatact ccaatggaaa tcgctaggca
    13921 agagataaac tttcagatat ccgcagagag atcatcgcct ctttttgtcg caagcattct
    13981 cctctcctag tcattttcta ccttatcttc tacctgagga tagagagttg ttccccaaat
    14041 agaaatcgtc cgcttacgca ctagtggcaa atcggttttt tcataaaccg tacgccacca
    14101 ttcccaggca agcccggtac actctctaat tttgacagag agattacgaa cattcccttt
    14161 taaaggaata ctagtggtaa agtgagccgt taaatcctgc ccatttctgt cccaagcctt
    14221 aggagtcaag acttccttac cttgatgatc ataggataat tcattccaag taatataata
    14281 ttgggcaaca taggcaccac tatgatccag cagtaaatct ccgtttctgt aagctgtaac
    14341 cttagtctca acatagtctg tactattttg aaaggtcgca actacattgt cacgtaaaaa
    14401 agaagttgta taggaaatcg gcaagcctgg atgatctgct gtaaagcgac tgccttcttg
    14461 aatcaagtcc tctaccatat ccaccttgcc tgttacaact cgggcacccg aacttgggtc
    14521 gccccctaaa ataaccgcct tcacttctgt attgtccaaa atctgcttcc actctgtctg
    14581 aggagctacc ttgactcctt ttatcaaagc ttcaaaagca gcctctactt catcactctt
    14641 actcgtggtt tccaacttga gatagacttg gcgcccataa gcaacactcg aaatatagac
    14701 caaaggacgc tctgcagaaa ttcctctctg ttttaaatcc tctaccgtta cagtatcttg
    14761 aaacacatct cctggatttt taacagcgtc tacgctgact gtataataaa tctgcttaaa
    14821 attaacaatc tgaatctgct tttcgcctga atggacagag ttaaaatcaa tatcaagaga
    14881 attccctgtc ttttcaaagt cagaaccaaa cttgaccttg agttgttcca tgctgtgagc
    14941 cgtgattttt tcatactgca ttctagctgg gacattattg acctgaccat aatcttgatg
    15001 ccacttagcc aacaaatcgt ttaccgctcc gcgaacactt gaattgctgg ggtcttccac
    15061 ttggagaaag ctatcgctac ttgccaaacc aggcaaatca atactataag tcatcggagc
    15121 acgatcgacc gcaagaagag tgggattatt ctctaacaag gtctcatcca ctacgagaag
    15181 tgctccagga tagaggcgac tgtcgttggt agctgttaca gaaatatcac ttgtatttgt
    15241 cgacaagctc cgcttctttc tttcgataac aacaaactca tcgggtagct gattaccctc
    15301 tttgatgaaa cgattttcaa tactttctcc ctgatgggtc aagagtttct ttttatcgta
    15361 attcatagct agtataaagt catttactgc tttatttgcc atcttctacc tcctaataag
    15421 ttcctggatt gagttgcata aactcagact tgttcagcga aatcagccgt ggttggacta
    15481 agtgatccaa aatttcctcg tacaattctt ctgagacatt gcgtcgccgt ctggctagat
    15541 aagaagtcgg aataaccgta ttatccaaca taaatacctt atctaagtca atcaaggttg
    15601 gtcttgtaaa aggattacga gctagatccg gctcttctat cataaagttc ttgaccaaac
    15661 gtctggtcaa gagagctggt ttgaaggtct gatttttaac caactctttg tttttagtca
    15721 tgctgttgtc aatacagata tacatatgat tcttcacagc caaatcgcta ctaatagtcg
    15781 gaaaaggcaa ataaagaact acaacatctc ctctcttaat caagcaagag cacccccttt
    15841 tctcctaatg taacatagac aggattgacc aagtcttctg attgactcag aatttccaaa
    15901 gtttgagttt ggcgcgctgt caatttagta gcatcttgtc tcttcaatac aaaatgcttg
    15961 tcgccaataa ccttgacaat ataatccttc tccaaagctg actggtaaat ccacatcaga
    16021 tgttgtctgt cctgagaact caagagagaa ggattttcaa gcctcccgat agtctgataa
    16081 aaatcaaaaa caggagctaa ctcctgccaa tctgattggc tagttgtcaa ggctagaaaa
    16141 agggctttgc gagctgatac ttcttggtta gccttgagag ttactttccc ctccaagttt
    16201 tttagaaatc gggaaactcc agaaagcaaa tttttctcta actgcgagaa ataaaaacct
    16261 ttcgttccca gacataagtc tttcatgtcg ctttctctag caaataagag ctcaaacatt
    16321 tgatagtaaa agaaaaatat ctggcactgg gtcgcgctca tcttttcctt atcggcttct
    16381 ttttttaacc agagcaaggg cgacaggtag ctggattgag acatttcctc tacctcctac
    16441 tcttttttaa ctggagcatc tgcactagct gccacttctt ttgactggat actttcccac
    16501 tggttaatct cctctgagat aagaccttcg catgtcttga caaatagggc aaaagccttg
    16561 gtctttcctg catatttctc cgtttggcat tgatagagga atttttcttt ctccaggagt
    16621 tgcgcagttt tttggtaaga aatccaattt tcctttgcat tatacaaatt gataatcccc
    16681 tcacacagca agccgagact ggataaggca accgaaatca aacggtagcg atcacctggc
    16741 ataggaatag cacaaaagac agctatgagg aaacctgcca cgatttctgt tatttttaat
    16801 accttatagc gtctacgatg ttgaacgctt ttctttaaaa aatgagctat ctgtacgtct
    16861 aatcgctctg tcaggtacat ttcttctggc gtcatattcg taactccttt catttacttt
    16921 gataaccata gtataacata tttgaatatc aaagtatagt aaaaactgga ctataactta
    16981 ttagacttcc tgcgaaacaa aatatggtat agtagttcta tgaattatga agcaagtaaa
    17041 caactaactg atgcacgatt taagcgtctt gttagtgttc agcgtaccac ttttgaagag
    17101 atgttagctg tattaaaaac agcttatcaa aaaagtcgga caagctggta aaatcttggc
    17161 tgacagtggt tatcaagggc tcatgaagat atatcctcaa gcacaaactc cacgtaaatc
    17221 cagcaaactc aagccgctaa cagttgaaga taaagcctat aaccatgcgc tatctaagga
    17281 gagaagcaag gttgagaaca tctttgccaa agtagaaatg tttaaaatgt tttcaacaac
    17341 ctatcgaaat catcgtaaac gcttcggatt acgaatgaat ttgattgctg gtattatcaa
    17401 tcatgaacta ggattttggt ttcgcaggaa gtctattata tatgcacaga catatgaaat
    17461 ctttttgaga cagcagttat gatatattta tccattcaca acatcttctg tactaatact
    17521 acaatattgg tacattttga ttacacaatc gtttcaaaaa atatgatgga agcctgagta
    17581 ttttatagta attcccttcc tatcaactag atcaaccagt tgagtgttga atgttgaaac
    17641 accctagcct cagcaaagga ctatgatcag catttcctag ccaataggac gctccaaatc
    17701 acctattcta agtccatcac tagcttggtc agtaagatac agactgcaag gcgagactga
    17761 cgtggttgat gagattttgg aagagtatta caatcataga ttcaccagta gacttgaccg
    17821 cagatttaac tatagtagat tgaaactaga atagtacatc tctgcttcta aaacattgtt
    17881 agaaatcgat ttgactgtcc tgatcaattt gtcctgttct cgtttcattt cactatacta
    17941 aaaaaccacc tgaaacaaat caagtgacta gaggataaaa acaaagacta ggcaaaaagt
    18001 tttgaaaagc aaaaaacgca taatatcagg tcttgaaaaa ccttgatact atacgtttga
    18061 ttgtgggaag attgacttca ttttctcctg aaattgaagt tttttctagt ttccagatat
    18121 agacgaattc aatatagcca tctcgctaca attattttaa ccatcgagaa aatttttcag
    18181 aatttcaaaa atttcatttt gatccaatga aatgtcatca ttttgaagaa tagttgactt
    18241 cacaggctct aattctatca caaagcggtg tatgtctgct tttacactac ttgatgttat
    18301 agccgataac ttttcatctc ctagcaagga agctgtcaaa cgggtaatgt cattcaaatg
    18361 ctttttaatg gacttactta aaccttgagc acctgtagca gagcgttccc tcatatccag
    18421 ccaagcttta gccttaaaaa taattaatac tcaatgaaaa tcaaagagca aactaggaag
    18481 ctagctgcag gctgctcaaa acactgtttt gaggttgtag atggaagctg acgtggtttg
    18541 aagagatttt cgaagagtat aaaccacaat tactcaatac cgaatacccc tgaatggttt
    18601 ctttttcatg caccaatata ttataataat cttcatccaa taataaggct gataaactag
    18661 catcttggtc aaaatgtaag ggaatttctc gaccgtcctt ctttaatgga tattctggta
    18721 agatactaaa tagttcaatc atagaaggaa actcaggatt agttgttgta aatcgaaaaa
    18781 gctgcgcttt ctcatctttc tgacttcctt gatactctcc caattctaaa aaatgattca
    18841 aggtagtata aaattcctta ttttttactt cataaatgat gaccatatca taatcttttg
    18901 ttgtgcgact tttaaatcct tgcgaatcca atacgataga ggtagcagtt cccccaatca
    18961 gaacataata gttctgaaaa tccgcaaaca tttcttgaaa aataactttc gtattaactg
    19021 gcatcatctt ctcccagata ctgtaatatc atattttcta gtgcttcact ctcttcctct
    19081 atacgtgggt catcatcatc ttttaaggtc aaataaagag aaatcggatc tacaaattgt
    19141 ttatcatgat tatttttaaa atcattccaa aactcagata caaaaggacg atatttccat
    19201 atctctagca tctttccttt taaaacatgc tgagaaagtg gcaaggataa ctgattgaat
    19261 tttctctgcc atatgacata gctagtattt tcatccgttt cagctaaaaa agttgaatgc
    19321 gacaaagcat aagcaccacc atatagaagg ttagaaacag attttatctg ctttatatcg
    19381 ccatctggca ataaaaaggc aatccacgtt aattgttcgc taggtgttaa ttccttaggg
    19441 atttcagtat cattcgcatt gagtactagt cccaatggag ggaagaagag gtttccctta
    19501 aagtctacaa acggaactct agcttgaagt aattgctttt tttcactgtc tgataacttc
    19561 gaaaacacca aaacaacatc catattggct ttttcaccca tagtgcgagc ctgagtaaca
    19621 aatgaactca aactccccct tctcttttct ttaatcaata aaaatgattc cttatgaaaa
    19681 gtcatttgag tatactcaaa cctttttaca aaactgattg gtaaagtcaa agtcagttcc
    19741 tcaatactga catcaacata aaaggattga aaaattgaca ttatcttttt gacattcatg
    19801 ttttttcctt attctatcat ttttattata ttttatcatt cggtataata aaatataagt
    19861 tttttgataa aataaccaaa aactactcgc tatcccctct cacattaccc tacatatcgt
    19921 ttgacatgcg actgatagtt caggaaaact tcaaggagaa cttttctctc atccactatg
    19981 caggacttac tatttcactt ctactcctat aggctcaatt tgacactttt ctttttcgaa
    20041 ttgctcatat gcctcctgaa tagtgagttc gatttatcca aatttatttt tgtctatttt
    20101 gatgaatact tccacgaaga tagtctgaaa atgaatttgc accaattttc cttctctttt
    20161 taagcatatt attccagata tgattgccct cttgttcagt aataaattac gctttaaacg
    20221 cttcaatcag tatatctcct gttgtcatat gttctaaaga aaattcttct acatatggtt
    20281 taacatctct taggttatta cttcctaata tcccattatg ctttttcgct aagaaaatag
    20341 aagtcgcttc tcccttacca ataatcttgt tactatcata atttcttgtt aaatctctat
    20401 ataatgcgta ttcttcagtt ccaatgtcta tgctcacaat ctcagctgaa cccttagtta
    20461 ccaactggtc tatcctagat tttaaatggg gaattgtagg tatattgatt tcatcataca
    20521 cctcttgtgg aataacaatt ttacccgaat agagcttttc taaaagatgt tcagtgccaa
    20581 cccataaaaa tgctgaaata caatctgcat caataaatac acgactagtc aaacgacaac
    20641 tccccctctt catctagccc atatacaata tcatatctga aagcatctag taacagttcc
    20701 tcatacttcc cttgcgaaat tctgttattt tctaaaagtt gttcagtcga tttaatatag
    20761 tgtcctaata ccatttcctt tttactttct gacaaaggac gatataaact tgtatcatag
    20821 cctaatcttg aagctgtctc tataacacta atatccatat ttttaatttc ttctgcatca
    20881 aggtatccat catttctcaa tctatataac atagctttat gactgatacc ataaaactga
    20941 cccaatttta taatatcttc tacttcaaga tgagttctat tggcattttc tctgatttcc
    21001 tcaaccatcc tatacagtga agatgggaaa attaaaaaat aagaagcaaa ctgatccgct
    21061 tttctttcag tttcatctcc ttcaccaatc aagataagac tgactgaact cttcttcacc
    21121 tcatcataat aaagatgata cagtccatgt gctaaagaaa atctttacct tcctaatggc
    21181 atgtctgaat tgactgcaat gagactgaac tgagttcctt tataacagac tccgctaata
    21241 ttctttccga gtccataaaa taccagcgtc aaattttcta tcttttgtac caatttaaaa
    21301 atatctatcg gcgattcacc atcagctccc aacttttttc taagatttga agctttatta
    21361 cttaaatcca ttcgtgatat tcctttcacc tttttccatt ttggcaatca tgctttgatt
    21421 caaagacaaa tactcagcaa tcttatcttg agtaagattt gaagatattc taagttcttt
    21481 tattcttttt ccaacatcac atacatttat catattcata cacctctcat aaaaagaata
    21541 gcacattctt gtcataattt tttaaataaa aaaattatga caaaacaagg aagcaattta
    21601 ttgatgctac ttaaaaatct aaaattgatg atattaaacc tatttgatga gttcctatca
    21661 aaaatcgtat cttcaacctc aaaacagtac ttaaagctat ccgactcggt ttacattgtc
    21721 aaatttagat tttatttgag cataactttc tagtttgctt tttgattttt gtttaatata
    21781 gtaacaaaaa ttgaaggaaa tatctccaca agaaaacgca tactattaag ctttttcaag
    21841 acctaataat atgcgctgtt ctgatttgaa agacattcca ttattatttt actgtaatca
    21901 agccatctgg ctctactgtg aattctggct tgtctgccag tgttccgtct ggtttgaggt
    21961 agtaccagcc tgttccgtcc gctgactgga taaaggcatt tgataccatg gcgccttctt
    22021 tagcgtctaa gtagtaccaa gtgtccttgt acttgaccca gcctgtcttc atggcacctt
    22081 cttcgttgaa atagtaccac ttatcagcga ttttcttcca gcctgtagcc atttcgcctg
    22141 agttgtcgaa ccagtaccag ttgccgtctg tgtgcttcct ccagcggtct gcaagcatat
    22201 agcctgaact gtcaaagtag taccaagtgc cattgatttt ctcaaacttg tcttttggat
    22261 aagagccgtc tgaatgtacg taccagtagc cagtgtcatt cttctgccag cctgtttcaa
    22321 tcgtcaagcc gttctcaata tcatgcttaa actgctcacg gctaatgccc catttagcaa
    22381 gatatggata agggtcaacg tggtctgagt ggttgtttgg ttggttattc gtgcaatact
    22441 cgtgcgtttt aattccagct aaactccctg tatcaagcgt tttcggcaaa cctgcttcat
    22501 ctgctagatt gcgtaagagt tcgatataaa ggcggtagtc cgtcatgaac tcttctttgg
    22561 ttgaatggct ttcaataagt tcaaccgctg cataggtctc agcattccaa ccgcccccaa
    22621 cgtcccaggc accattatca acaggtccta cctgcatgat gcaaccgttc ccaacaatgt
    22681 gcgagaaaaa acctaattct gggtctttcc gccagtgata atccgcttca ttctgtacgg
    22741 ttgaatgcgg attcccagtt gagtgtgcgt gtacttgcct atatggttgc acgccgactt
    22801 gaggcaaatc tgttcttaat ttactcacat taatttccat attctactcc ttatcaatta
    22861 aaacaactca ttttttacaa tccagaacca gaaactcctt tatttctacc ttacaaagaa
    22921 gacaatctta gtcacgatta ggcttgtaga tagaacttca aaacgcacta ttttgacact
    22981 gtaaatagga ctgacaaggt ctgcattcta tctacaataa caccccagac taaaaagctt
    23041 ttcaaaagta tatttttaca gtctctatat gtccttttca taaatactat actttattat
    23101 atcatataaa agaagtcaaa agtctgttaa actattttca acaccaaact aaagacgaga
    23161 acacaagttt tttcgatttt cactagagga aatggatttt attcagtaaa tccaactagg
    23221 attgcacttt ggttgccaag attgccttgc cttcttttat caagggatga cggaataatg
    23281 agaagtacag ttgaactgtc atggcaaccc agataagagg ttcacaaagg ataacaccct
    23341 tatatcctgc ccaaggaata atcaaaacca caaaaacgat ttttccgatt agttcaataa
    23401 agctagaaac tagaggaagg atcttttgcc ccaagccctg caagcaattg cgataaatca
    23461 acaagaggct caaaatggga taaaaggttg aactgatttg cagatagaga cttccatttt
    23521 ctatcaagta accatctgtc gaactagcca agaaggaaac caaagctgga ctggcaaaaa
    23581 agaggaaaat acaaacaaaa actgcccagg atatacttaa acgactgccg attcgaagac
    23641 cttgaacaat gcggtcaggt tgcttagctc ctagattctg agaagcaaag gtcgtcattg
    23701 atgcagaaat agcggtcata ggaagaaggg caaaggtcat aatgcgtcga gctgccgtct
    23761 gggcactaat aatcactgca ccaaatgtat taacagaaga ctgtaaaatc acactgccga
    23821 tagatacaat tgaactcatc aagcccatag ccaaaccttg ctccaagaga tctgcgtaca
    23881 agcttttgtc ccatttgaaa tgtttaaact gtggcaagag ttctggcaca cttttacgaa
    23941 tataataaaa gcagagaacc gctgataaac cttgcgaaat aatggtagca agtcctgcgg
    24001 attgaactcc cagatgcaat tgcgtaataa aatagagatc cagaaccaca ttaaccaagg
    24061 cagagaaaat cagaaatccc agggctgcta gactgtcacc aatagaccgc aacaagcctg
    24121 caaaaagatt ataagcaaag ctgacaccta cacaggtcac aatcatagaa atatattgat
    24181 aagattgagg aagaatttct gcaggagtat ctaagtattg caagagagga tacaagccaa
    24241 gaaagcccag caacataact agaatgctca aaagagcacc taaaatccaa gtagctgcta
    24301 ctgcttcctt gattttagtg aaattccgag ccccataata acgagcaatg acaatcccca
    24361 tgccattgcc aacaccaagt gtaaaaccta caatcaggtc aaaaatcgct gtcgtcgctc
    24421 ctactgcagc caaggattct tgaccaagaa atcgtccaac aatcaagaca tcagcagtgt
    24481 tatagagctg ttgaaaaata tttgatagca aaattggaaa ggtgaagctt aagagcgagg
    24541 gaagaatcgg accatgtatc aggtccactg ttcgtttttt attcataaag ctattatatc
    24601 agctttccag aggagcgaca agaaacagca aacttatata tagcggaatg aaataaggaa
    24661 agtcaaatcg atttttaaca atgttttaga agtagatatg tactattcta gtttcgatct
    24721 attatagttg gttgagtttg gaatagtacg ctgtagctgc taaaacattt ctagaaatta
    24781 atttgacttt cctaatagag ttgttcatat cttatttcaa gctactatat ataatctaaa
    24841 aacttgaatc aaaaatcgaa aaagtagcgg atttctccac tgctttaaca gcttattctt
    24901 caatttcgat ttcaagttca tcgcctaagt caagcgtaac ttcttcattc actgcttctt
    24961 ctttctttgg ctcatctttt ttgttttcag tatcttgttc tgaaacttct tctccatcaa
    25021 tcaagccaaa tttagaacgg acttgcttat caatttcatc aaagatttct gggtgctctg
    25081 ccaagtattt cttagcattc tcagaacctt gcccaatttt ttcatctttg taagaatacc
    25141 aagcccctgc ttttttgata atatccaaat cgcttgcaat cttcaaaagc tcaccagtct
    25201 tagaaattcc ttctccgtac ataatttcaa ctacggcttc cttaaacggt ggagctacct
    25261 tattttttac aaccttaatc ttagtttctt taccgacatt ggtttctttt tggtcaccag
    25321 ttcccttaat ttgtgtatta ccacgaacat ccaagcggac tgaagcatag aatttcaaag
    25381 cacgtccgcc cggtgttgtt tctggatttc caaacatcac tccaactttt tcacgcaatt
    25441 ggttgataaa aatggcaatt gttttggttt tattgataga ggcgccaagt ttacgcatgg
    25501 cctggctcat catacgagcc tgcaaaccaa catggctatc tccgatatct ccatcaattt
    25561 ccgcacgagg aacaagggca gcaactgagt cgactacgac aagatcaact gcacctgagt
    25621 caatcaattt tcccgcaatc tcaagacctt gctctcctga gtctggttga gacaagagca
    25681 attcgtcaat attgacacca agggccgcag cataagctgg atcaagggca tgttccgcat
    25741 cgataaaggc agcaatccca ccttcttttt gcgcttgtgc aactgcatga agggcaaccg
    25801 ttgtcttacc agatgactct gggccataga tttcgatgat acgtccctta ggataaccac
    25861 ctgagccaag ggcaatgtca agagctaaag aacctgagct catcacttgc accttttgct
    25921 ccgcacgttc acccaaacgc atgattgatc ctttaccaaa gtctttctca atcaatttaa
    25981 gagcatcatt caaggccttt tcacgttctg ccccaaattt ttttgaaatt tcttctaatt
    26041 tttttggttt tttcgccatt ctattctcct acattctaat gatcctcaga cctatctact
    26101 attatatcaa aagttagtca cttaataaag ccttgcgaac taggttaaag gcatgcataa
    26161 ccgcaatatg acgtacatct gctcggcttc tgcctccaat attaaccttg ataacctcag
    26221 ttccttgctc ttgcgccaag cctatgaaga ctgtcccaac tgggtgtcct tctaggctat
    26281 ctggtcctgc cactccggtc aaactaattc caaaatcaga ctgggtcttg cttcgtgcct
    26341 gctcagccat cttctgagct gtaaattcag acaccacacc atgttcttcc aaattcttgg
    26401 caggaatatc caacatcctt gatttttcct ccaagctata ggtcacaaaa ccacccttaa
    26461 atatacttga agctccagaa aaattcgcca cggtagcttg gaaaagacct gccgttaaac
    26521 tctctgcagc cgcgatggtt ttcccttgcc ttttcagttc ttctaccaca atgctggcta
    26581 aactagtttc ttccccataa ccatagcaaa agtctcgtaa agaaattcct tcgaaagtct
    26641 ggcagtccaa gatttgattt tccaagatat ccagcgcttg attcgcctct tcttgactgc
    26701 tagcctttgt tgacagacgt agagtgactt ctcctgtctt ggcataaggg gccaaggtag
    26761 gatcgatctg attatcaatt aaatcagcca aaatcgtaac cagctggctc tcgccaatcc
    26821 caaagaaacg aagaactcgg gaatacagct tgctccctgt catcaacttg ggtagaagtt
    26881 ggtttaagac catgggtttc aattcacttg gcggacctgg aaggacgaca taggtcactc
    26941 cgtctacttc taattttcct ccaacagcca gtcctgtttc gtttggcagt ggaatcgctc
    27001 cttctacaat ttgagcttgt ctttcgttat tcggtgttcg ggcatagtct ggtcgcaggg
    27061 caaaaaagat atccaacttc tcctgagcct gaggatcaaa gactaatgct ttccctaaaa
    27121 atttagctag ggtttgtttg gttaggtcgt cctcagttgg ccccaaaccg cctgtcaaaa
    27181 tcaccagact gctacgttga ctggcaatct caagcaaaga caagagacga acttcattgt
    27241 ctcctacagc cgtctgaaaa tatacatcta ccccaatctc agctagtttt tccgacaaaa
    27301 actgggcatt ggtgttgaca atctgtcctg tcaaaatctc tgttccaaca gcaatgattt
    27361 ctgctttcat gtttcctcct acctatctat tcgtattttt ttgaaaaaat cgcaggaatt
    27421 ttcctacgat tgattttttt atttgtatca aaagttaatt atcttcatca ccaacaggtg
    27481 ctctgccaaa taaatcttca aataaaaccg cattggtttc aagctgagta acttcttctt
    27541 gtcccaaaga acgtcggagt atattttgca tttccaacat atgtgctctc gaaacaatct
    27601 ggtaagaaac accttgaagt atctctcctt caccctgcaa ctgctgagtt tcaatggttt
    27661 taaatgaatc tttatagcct agcaagttag ggatactttt tgcagacaaa tcaatattgg
    27721 tctgcatatt gtcactcaaa gcttttagaa tctcttgata atgaccaacg ctatttaaac
    27781 tgagagcttt ttccatgact ttttgaataa cttcacgttg acgtttttga cgaccataat
    27841 ccccctcagg atcttggtaa cgcattcgtg catagactag ggcttcttct cccccaatat
    27901 gttgctcccc aacaccgata gaaatggtat taaattcttc ttggtcactg atagaaattg
    27961 ggaaacctag gatattattg actgtaatac ctcctactgc atccactaat ttttgcaacc
    28021 ctctcatatt gaccatcaca tagcgatcaa tatggatatt catcattttt tgaatggttt
    28081 ctatagcaag ctctgctcca ccatctgcat atgctgagtt cagtttcgct tcatgagcct
    28141 gaccattccc tgattcaatg cgcgtcagaa tatcccgctc taaactcatc attgttgttt
    28201 ttttcgtttt aggattcact gtcatcaaga tcatgctatc acttctaccg acccaagttt
    28261 cagttcgttc aacatttccg gtgtccactc ccattaacag aatggttaga ggttcagtcg
    28321 cttcaataac cttggtttct tcgccgattt ttttataggt tttagctaag gtttctgtcc
    28381 cttgttgata aatagtataa gcaaaaacac ctactcctac tacagttaca gaaagtaaag
    28441 ctagcaccat tccaataatt tttttaacca tatttctact aacctatcag tttacccatc
    28501 aagtaaacat cgataaattt cccttcttct atatatgccc cacgctcttg gctaccttca
    28561 atgacaaagc catgcttttg ataaagatgg actgctgctt gattacgagt ttggacagtc
    28621 agttggagac gacgcagaat gccacctgct tgtgcccact ctatcgcttc ttctagcaac
    28681 aaacttccca agccattatt ccaatatctt tttccaatca caatgaagag atccccaata
    28741 tgacggactc tcttacgctg gtcagctgta atatttacaa taccagcaat tttgccattt
    28801 aagaatgcaa gtaaggttat ctgattgtcc gaactagctt gcttgttgag gaatatttcc
    28861 atctcctcac tagtcaagag aataccatct ccgtctaggc tggtaaagtc tgtctccaaa
    28921 ctcacacgat ttaaaaaggc cactaattca gctgcatctt tgggctctgc ttccctaatg
    28981 agcaattcat actccatatt gaagctcctc taacaatttc tcagcacgca aaccctttgc
    29041 ctgaaaattt aaacggcgtc catctgcttc ttttagaatt tccaattcta aataagtatc
    29101 tggcaaggca tctcctaaga gatttcccca ctcaataaca gtcacaccgc caccaaagat
    29161 aaactcatcc aagtcgatag aatcagcatc tccttcaata cgataaacat ctaggtgata
    29221 aagtggaagt cgaccttcat actctctcac gatagtatag gtgggacttt taatcatttg
    29281 agaaatctgt aatccttttg caagtccttt agtaaaggtc gttttacctg cacccagttc
    29341 tccagttaag attaaaacat cattctttgc taatagatgg cccaaacgct cccctaaggc
    29401 ttgcaactct tcttcatttt ttgtgtacat attcttatta taccaaaaac ttttcttttg
    29461 tgtctatttt cctactaaac ttatcatcat aacatccata aaaaacaggc tttctctaaa
    29521 agaaaatgag cgtaacaatg accaatacaa gatctcggaa aatatgacca taaaaggaaa
    29581 cttccttctt aaccgaattt gggacaagat aggctgcaaa aaacaagccc agtccaatat
    29641 aaatcagaag tgagacaatg gtcattggat ttcttaagaa aagaagtgtt gctaaaatag
    29701 tcaccaacac tgtctttttt ctgtctagca tagcaagaaa atcgcgcacg tattttttca
    29761 agggtaaaaa aatcagcaaa tctagcccaa ataggaaaaa gaaggatggc aataaaaagt
    29821 caactaattc ttgctgcagc gtatttttga tgaacaagtt atctgacaaa acaagaacag
    29881 ctcctaacaa attaattaag agtaacatac tgtaaaaaag cttcacagac ttcttactgg
    29941 ctaggacact atggacttct tgcttacggg tataaagata atttactcca gcacagattc
    30001 ctgaaacgaa aaccatgctt ccgatgaaaa aaactgtact ttgtttaaag gacaagatgc
    30061 attccttcca taggaaacag ctactcaaac tgatttgaat taaagctaac aaaaataaga
    30121 ttctcattga tttcatcttc tctctccctt cctaccaatc attatactag gagaaaagag
    30181 agaactgttt ctaatcttct caaatgtctc tttaagacgc taaacaaaca ctagagacta
    30241 atactcaatg aaaatcaaag atcaaactag gtagctagcc acaggttgct caaaacactg
    30301 ttttgagatt gcagatagag ctgacgtgat ttgaagagat tttcgaagaa tataaatttg
    30361 aaatcatgaa aatccgtcaa acgggtggtt gttttgtctc gcacctcacg gagcgagacg
    30421 gactcagagt cacataatta taaggctgat agtattaatc taactatcag ccttacaggt
    30481 tatttaacgt ttcagaaaaa ctataatgtc aagattaact aaacagtatc taattccttc
    30541 aaataatttt ctatcttcat caacattaaa ggattgttat aaatcttaca taactctctt
    30601 gcttctatat aataattttt gacttgttct ctgtctagaa atttggctcc agcatttcct
    30661 acaagaataa gtagaggagc caattggtag cttgtctgtc tttgtttaca gagttcaatc
    30721 gtttcaagag cttcttggat ggcttcatta tatttttcct ttgatactag gtagtgagcg
    30781 tagttgtaac gaactctgat gtagccaaat aaaaactctt gatggtccaa attttttgtc
    30841 tgatacaact ctattaaatg agagtagttt gcctcatatt cttgttcacg acccactaag
    30901 gaatagaaat tagatagagt attcaacgcc tttaaataaa tcagagtatt tgaagagact
    30961 tttaataata tattttccaa tgaagaaatt gcctcacact tactgtcata ttgatagaag
    31021 tcaataatag ctttaatcca ttcaaggtaa gttcggtctt ctaatgttag aaaagtgctt
    31081 cgttctacct ctattttata aagatattct aaatcgtcat aatttctgtc atctaatagg
    31141 cgagcagata gatgtttgaa attagagagg ttagacttaa cttcgatttg ttcattgaaa
    31201 aagtaatcca aagggacttc aagtcgttga gagagtttga ataacaagtc tgcggaggga
    31261 ataaaatgac ctctttcaat tttactaatc tggctttgtt cacaaattcc ttctgcaaga
    31321 gtttgttggg agagtctcaa ctcttttctt ttcaatctga atttctctgc gagtgtattc
    31381 attaaagata ataaaccttc ctatttgctt aatttcatta taaagttttt agttcaaaat
    31441 agcaagttaa aggaataaat aattctttat ggtaataaa
//
LOCUS       DAVKQL010000024        31173 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVKQL010000024 DAVKQL010000000
VERSION     DAVKQL010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31173
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.024"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(1..881)
                     /locus_tag="VW561_001004"
                     /pseudo
     CDS             complement(1..881)
                     /locus_tag="VW561_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1250..3220)
                     /gene="tkt"
                     /locus_tag="VW561_001005"
     CDS             complement(1250..3220)
                     /gene="tkt"
                     /locus_tag="VW561_001005"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU9943282.1"
                     /translation="MSLQSVNAIRFLGVDAINKSNSGHPGIVMGAAPMAYSLFTKHLR
                     ITPEQPNWINRDRFILSAGHGSMLLYALLHLTGYNDVSMDEIKNFRQWGSKTPGHPEV
                     THTSGVDATSGPLGQGISTAVGFAQAERFLAAKYNKDGFPIFDHYTYVIAGDGDFMEG
                     VSAEAASYAGHQALDKLIVLYDSNDICLDGETKDTFSENVRARYDAYGWHTVLVEDGT
                     DLAAISTAIETAKFSGKPSLIEVKTVIGYGSPNKSGTNAVHGAPLGAEETGATRKFLG
                     WDYDPFEVPEEVYSDFKTNVADRGQEAYDAWASLVSDYKVAYPEVASEIDAIVAGKSP
                     VTITEKDFPVYENGFSQATRNSSQDAINTAAAVLPTFLGGSADLAHSNMTYIKADGLQ
                     DKYNPLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAIRLSAIQEL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRSMPNLTVIRPADARETQAAWHHALTSTT
                     TPTVIVLTRQNLVVEEGTDFGKVAKGAYVVYDTPGFDTIIIATGSEVNLAIKAAKELV
                     LQGGKVRVVSMPSTELFDAQDATYKEDILPSKTRRRVAIEMAATQSWYKYVGLDGAVI
                     GIDIFGASAPAQTVIDNYGFTVENIVAQVKSL"
     gene            complement(3257..3952)
                     /locus_tag="VW561_001006"
     CDS             complement(3257..3952)
                     /locus_tag="VW561_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU9943283.1"
                     /translation="MSRIEFSPSLMTMDLDKFKEQITFLNDKVASYHIDIMDGHFVPN
                     ITLSPWFIQEVQKISDTPLSVHLMVTDPTFWVDQVLDLQCEYICIHAEVLNGLAFRLI
                     DKIHDAGLKAGVVLNPETPVSTIFPYIDLLDKVTIMTVDPGFAGQRFLESTLYKIQEL
                     RQLRVQNGYHYIIEMDGSSSRKTFKQIDVAGPDIYVIGRSGLFGLDDDIAKAWDICSR
                     DYEEMTGKTMPIK"
     gene            complement(3963..5048)
                     /locus_tag="VW561_001007"
     CDS             complement(3963..5048)
                     /locus_tag="VW561_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEU9943284.1"
                     /translation="MLKHLNLKGHLLTAISYMIPIVCGAGFLVAIGLAMGGGVPDALV
                     AGKFTIWDALATMGGKALGLLPVVIATGLSYSIAGKPGIAPGFVVGLIANSVGSGFIG
                     GILGGYIAGFLVQAIIKKVKVPNWIKGLMPTLIIPFVASLVSSLIMIYIIGAPIAAFT
                     NWLTSLLQSLGSASNGLMGAVIGVLSAVDFGGPLNKTVYAFVLTLQAEGVKEPLTALQ
                     LVNTATPVGFGLAYFIAKLLKKNIYTQEEIETLKSAVPMGIVNIVEGVIPIVMNNLVP
                     GLIATGIGGAVGGAVSLTMGADSAVPFGGVLMLPTMTRPVAGICALLANIVVTGLVYA
                     ILKKPIKHTEPVMTVEEEIDLSDIEIL"
     gene            complement(5074..5385)
                     /locus_tag="VW561_001008"
     CDS             complement(5074..5385)
                     /locus_tag="VW561_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEU9943285.1"
                     /translation="MKIVGVAACTVGIAHTYIAQEKLENAAKVAGHVIHVETQGTIGV
                     ENELSQEQIDAADVVILAVDVKISGMERFEGKKIIKVPTEVAVKSPNKLIAKAVEIVT
                     K"
     gene            complement(5413..5862)
                     /locus_tag="VW561_001009"
     CDS             complement(5413..5862)
                     /locus_tag="VW561_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9943286.1"
                     /translation="MEIKDILNVSLIQTDLQMQSKEEVFEALAQLLVETGYVSDRDQF
                     IEGLYQREAEGQTGIGNYIAIPHSKSSAVEKAGVVIAINHNEIPWETIDGKGVKVIVL
                     FAVGDDTEAAREHLKTLSLFARKLGNDEVVAKLVRAQTSDDVIAAFC"
     gene            complement(5840..6268)
                     /locus_tag="VW561_001010"
     CDS             complement(5840..6268)
                     /locus_tag="VW561_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9943287.1"
                     /translation="MNNLSLVLMDISVQNRQEAYKELANQISLLVSEDTEKIEELLYY
                     RERQGSIEVAKGVLLPHCEGNFQHHVLVITRLKPPIREWSKDIQCVDLIIGLAIAVSQ
                     DKSCIKTLMRRLADESFINQLKQLTKEELREIIYGNQRYS"
     gene            complement(6261..7724)
                     /locus_tag="VW561_001011"
     CDS             complement(6261..7724)
                     /locus_tag="VW561_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEU9943288.1"
                     /translation="MSRKQEQMETLLLLLRDSKDYISAKVLGEKLNCSDKTVYRLVKG
                     INKDCPVEAFILSEKGRGFKLNPRSSLVDVDGNFTEAFDPEVRREKLLERLLLTAPKP
                     HSIYDLGEEFYVSESVVLKDRQILQESLAIYGLDLKMRQRKLFIDGDEAQIRSAILNL
                     LPMFNQLDLEQITQNKVQPLDGELAHFCLGLLITLERELGVNIPYPYNINIFSHLYIF
                     ISRNRRSTSIHVVAPSKPTIVDEKIYSVCQKIIQEIEQYFRMKVDAVEIDYLYQYVVS
                     SRLQKPFSSGKLPFSQRVLDVTHYYFSRMCMDNREIETTDPDFVDLASHISPLLRRLD
                     NRVQIKNSLLSQILLTYPNLVKELTTISKEVSLVFGFASLSLDEIGFLVLYFARFQEK
                     RARPLKTVVMCTSGVGTSELLRARLEKQFTELDIIDVVAYHQLDELINLYPDLDFIVT
                     TVALQEPASVPFVLVSVFLTEGDKQRLQAKIQEINYE"
     gene            complement(8253..10589)
                     /locus_tag="VW561_001012"
     CDS             complement(8253..10589)
                     /locus_tag="VW561_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEU9943289.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAVASILTSYMA
                     HSEDKNPTAHAIRQRFQGQVVYPMLSNLPFSSDRKWGAMELEGLGIVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLNY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELIALAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRRSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEIATLTGQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            10722..11471
                     /locus_tag="VW561_001013"
     CDS             10722..11471
                     /locus_tag="VW561_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEU9943290.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPINRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYAGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            complement(11764..12378)
                     /locus_tag="VW561_001014"
     CDS             complement(11764..12378)
                     /locus_tag="VW561_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEU9943291.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(13194..13463)
                     /gene="rpsO"
                     /locus_tag="VW561_001015"
     CDS             complement(13194..13463)
                     /gene="rpsO"
                     /locus_tag="VW561_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU9943292.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            complement(13604..14233)
                     /locus_tag="VW561_001016"
     CDS             complement(13604..14233)
                     /locus_tag="VW561_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU9943293.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTSDFPEEFKSLAKDQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
     gene            15089..15229
                     /locus_tag="VW561_001017"
     CDS             15089..15229
                     /locus_tag="VW561_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEU9943294.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDFKWTVEEDLLALSNDIGN
                     FQ"
     gene            15236..15412
                     /locus_tag="VW561_001018"
     CDS             15236..15412
                     /locus_tag="VW561_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEU9943295.1"
                     /translation="MTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENF
                     LSDKEKQLNVRTRK"
     gene            15470..17413
                     /gene="thrS"
                     /locus_tag="VW561_001019"
     CDS             15470..17413
                     /gene="thrS"
                     /locus_tag="VW561_001019"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU9943296.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAHEIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(17462..18436)
                     /locus_tag="VW561_001020"
     CDS             complement(17462..18436)
                     /locus_tag="VW561_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU9943297.1"
                     /translation="MLDWKQFFLAYLRSRSRLFVYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLLLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(18429..19106)
                     /locus_tag="VW561_001021"
     CDS             complement(18429..19106)
                     /locus_tag="VW561_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9943298.1"
                     /translation="MHKILLVEDDQVIRQQVGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDNLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            19262..20335
                     /locus_tag="VW561_001022"
     CDS             19262..20335
                     /locus_tag="VW561_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEU9943299.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPRDPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILEAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            complement(20426..20728)
                     /locus_tag="VW561_001023"
     CDS             complement(20426..20728)
                     /locus_tag="VW561_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943300.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(20796..21233)
                     /locus_tag="VW561_001024"
     CDS             complement(20796..21233)
                     /locus_tag="VW561_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU9943301.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            complement(21309..22151)
                     /locus_tag="VW561_001025"
     CDS             complement(21309..22151)
                     /locus_tag="VW561_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU9943302.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADAQQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            22263..22913
                     /locus_tag="VW561_001026"
     CDS             22263..22913
                     /locus_tag="VW561_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEU9943303.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            23033..23740
                     /locus_tag="VW561_001027"
     CDS             23033..23740
                     /locus_tag="VW561_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEU9943304.1"
                     /translation="MKKSLKIFATSKWFDLFGVALVVGIAIASGYLNSRLDKFVDWGP
                     WTALVPFGLISVTNVGISMLSTRFTGKLSKWGNYFSIVNTILSGAIDYILGNKAAIIT
                     YPVTFLIYTFAIKKWKASQEGRPNQMSQKQVKLAVIIISIIAFLFAFVTNYIGYEGKM
                     NLLAYVTTIAFALSLIANALNALKLTTQWGFWLIYNFVQLTKAGIQGNFANIGKYIFY
                     ILNAIGALFVWNDEEVR"
     gene            23871..24284
                     /locus_tag="VW561_001028"
     CDS             23871..24284
                     /locus_tag="VW561_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943305.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFIIRLETSFEWLLLSGLIFFLA
                     QGLLLFLLIVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLRENYPDFYDKHISLWECL"
     gene            24303..24489
                     /locus_tag="VW561_001029"
                     /pseudo
     CDS             24303..24489
                     /locus_tag="VW561_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720032.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24756..25199)
                     /locus_tag="VW561_001030"
     CDS             complement(24756..25199)
                     /locus_tag="VW561_001030"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEU9943306.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(25229..27451)
                     /locus_tag="VW561_001031"
     CDS             complement(25229..27451)
                     /locus_tag="VW561_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU9943307.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRMIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            28445..29074
                     /locus_tag="VW561_001032"
     CDS             28445..29074
                     /locus_tag="VW561_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU9943308.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(29197..31089)
                     /gene="pepO"
                     /locus_tag="VW561_001033"
     CDS             complement(29197..31089)
                     /gene="pepO"
                     /locus_tag="VW561_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEU9943309.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
BASE COUNT         9247 a         7032 c         5687 g         9207 t
ORIGIN      
        1 atctgtgtag cacttttcca ttgttttaga gccttcaaat tggccttgaa tgagattctc
       61 tgccttctta ccaacgtctc ctttatgaga agaatatttt cgtttctgtc gcattttagc
      121 ttgtaaattg agtactttca tcaagccttg aactctttta tgatttacca gataaccacg
      181 atttcttagt tctaaatgaa cccggcgata agcataattt cccttgtgtt cgataaaaat
      241 ggattgaatt tcagctttaa gctcttggtc cttatctggt ttatctagct gtttcaagtg
      301 atagtagtag gtccaacgag ctagtttaat ggattttaga agaagatcta acgaaaactc
      361 agtcattaat tcttgaacaa tttctgtctt tcttctttct ctttttcctc cttcaatcgg
      421 agttctctta acttttttag gatggcattc tccgctctca ggtactcatt ttctgattga
      481 agacgttcta attctgtcct ctcttcaggt ctcttttttg gcttacgtcc cattttaggt
      541 actctccctc ttgttttctc aacaatagta tacccgtttt tcctgtattg tgctagccag
      601 ttaagaagta tcgtacgact tgggagaccg tattcaagag aaactctatc ttcagtccag
      661 ccttcatgtc agactttatg aatcatttct tgttttaaat caggagaata gtaacgattt
      721 tttccttttt tgacgaactc tattccgtaa cgatcaatca atttaatcat gtacctaaga
      781 ttagaattgt ttatcccaaa tttatttgaa agcttctcta agctatatcc ttgttttcta
      841 agttcataga tctgaacttt atcatcataa cttaatttca taataaaaca ccccaaaaag
      901 ttagattttt tctgtctaac ttttggggtc agttcatgta cacctgatat gatgcgtttt
      961 ttgaatttta tagaccttct ctctagcttt caaaaaactt acaatatcct aagtcaatct
     1021 gtggatgctg tgtttattga ttaaagaaac tatctagatc agtagcaaag tgattagcgt
     1081 acttcgcaac cgtttcaggg tatactctat tgtgtatact atctactata actttcgtag
     1141 ataaattatt acattctcat tatagttttt aaataaccaa tcatctgatt agtgtctatc
     1201 actacatctg ctagtctgac aacagctgta tcttcattgt aattggtttc tatagggact
     1261 taacttgagc aacgatattc tctaccgtaa atccataatt atcaatcaca gtctgagctg
     1321 gggcagacgc accgaagatg tcaataccga tgaccgcgcc atccaaacca acatacttgt
     1381 accaactttg ggtcgctgcc atttcaatgg ccacacgacg acgagtctta gatggtaaaa
     1441 tgtcttcctt gtaggtagca tcttgagcat caaatagttc ggttgagggc atagatacca
     1501 cacgtacttt accaccttgt aaaaccaatt ccttagcagc tttgatagct agattgacct
     1561 cagatcctgt agcaatgata atagtatcaa atcccggggt atcatacacg acgtaggctc
     1621 ctttagcgac cttaccaaag tctgtccctt cttcaactac caagttttga cgggttaaga
     1681 caatgacagt tggagtggtg gtactggtca aggcatgatg ccaagccgct tgagtttcac
     1741 gggcatccgc tggacggata acagtcaagt ttggcattga gcgtaaacct gccaaatgtt
     1801 caactggttc atgagttgga ccatcttcac caacggcaat tgaatcatgg gtaaagacat
     1861 aagttacagg caactcctga atggctgata gccgaatagc agctttgacg tagtcagaga
     1921 aaacaaagaa ggttccgcca taaactcgta aaccaccatg aagagccatt ccattgagga
     1981 ttgttcccat ggcaaattca cgtaccccaa actgaatatt gcggtttaat ggattatatt
     2041 tatcttgtaa gccatctgcc ttgatgtagg tcatgttgga gtgagctaag tctgccgatc
     2101 cacctaagaa ggttggtaaa actgctgctg ctgtattaat agcatcttgg gacgaattac
     2161 gagttgcttg agagaagcca ttctcataga cagggaagtc tttttcagta atggttacag
     2221 gggattttcc agctacaata gcgtcaatct cactagcaac ttcgggataa gcaaccttgt
     2281 aatcagacac caaactagcc caagcatcgt atgcctcctg accacgatcc gctacatttg
     2341 tcttgaaatc agaatatact tcctctggta cttcaaatgg atcgtaatcc catcccaaaa
     2401 acttacgagt tgctcctgtt tcttctgctc ctagtggtgc accatgaaca gcatttgtac
     2461 cacttttatt gggtgagccg taaccaatta ccgttttcac ttcaatcaaa ctcggcttac
     2521 cagaaaactt ggccgtctca attgctgtag aaattgctgc taaatctgtt ccatcttcta
     2581 ccagaactgt atgccaacca taagcatcgt aacgggcgcg aacattttca gagaaagtat
     2641 ctttggtctc accatccaag cagatatcgt tggagtcgta gaggacgata agcttatcta
     2701 aagcttgatg acctgcataa gaagccgcct ccgcagacac tccttccatg aagtcaccgt
     2761 ctccagcgat aacataagta taatggtcaa aaataggaaa accatctttg ttgtacttag
     2821 cagctaaaaa acgctctgct tgggcgaaac caacggcagt agaaatcccc tgaccaagcg
     2881 gaccagatgt cgcatccaca ccagacgtat gcgtcacttc aggatgacca ggtgtcttag
     2941 atccccattg ccggaaattt ttaatctcgt ccatggatac atcgttatac cctgttaaat
     3001 gcaagagagc atacagtagc attgatccat gacccgcaga caagataaag cgatctcggt
     3061 taatccagtt tggctgctca ggtgtaattc taaggtgctt tgtaaatagg ctataagcca
     3121 ttggcgcagc acccatgacg attcccgggt gaccagaatt agatttgtta atagcatcta
     3181 cgccaagaaa acgaatggcg ttaactgatt gtagtgacat atattcctcc taactaataa
     3241 atttcttctc aaaccattat ttgattggca ttgtttttcc ggtcatttct tcgtaatctc
     3301 tagaacagat atcccaggct ttggcaatat cgtcatccaa accaaataat ccactgcgac
     3361 ctataacata aatatctggt cctgccacat caatttgttt gaaagtctta cgactcgaag
     3421 aaccatccat ctcaatgatg tagtgataac cattctgaac tctaagctga cggagttctt
     3481 ggattttata caaggtagac tccaaaaagc gttgtcctgc aaaacctgga tctacagtca
     3541 taatagttac tttgtcaagt aaatcaatgt agggaaagat tgtagaaaca ggtgtttcag
     3601 gattaaggac aacaccagcc tttagacctg catcatgaat tttatcaatc aaacgaaaag
     3661 caagaccatt cagaacttca gcatgaatac aaatatactc acattgtaaa tcgagaactt
     3721 gatctaccca aaaggttggg tctgtgacca tcagatgaac tgataaaggt gtgtcactaa
     3781 ttttttgaac ttcttgaatg aaccaaggag acaaggtaat attgggaaca aaatggccat
     3841 ccataatatc gatatgataa gatgctactt tatcattcaa aaaagtaatc tgctctttga
     3901 atttgtccaa atccatggtc atcaaagatg gtgaaaattc aattcttgac atcgttaccc
     3961 tcttacaaaa tttcaatatc tgacaaatca atctcttctt caacagtcat aactggttct
     4021 gtatgtttta ttggtttttt caaaatcgcg tagacaagtc ctgtgactac aatgttggct
     4081 aacaaagcac aaataccagc tactggacga gtcatggttg gtaacataag cactccacca
     4141 aatggcacgg cagaatcagc acccattgtc aaagaaacag caccaccaac agcaccaccg
     4201 atacctgttg caatgagacc tggaaccaag ttattcataa caatcggaat tacaccttca
     4261 acaatattga caatccccat aggaacagcc gatttcaatg tttcgatttc ctcttgagta
     4321 tagatatttt ttttgagtaa tttcgcgata aaataggcca atccaaatcc aactggtgta
     4381 gcagtattca ccaattgcaa agcagtcaat ggttctttca caccttcagc ctgtaaagtc
     4441 aacacaaagg catagactgt tttattaagt gggccaccaa agtcaacagc actgagaact
     4501 ccaataactg cccccatcaa accatttgaa gcacttccca agctttgtaa taagctcgtc
     4561 aaccagttgg taaaggctgc gataggcgct ccaataatat aaatcataat caaactactt
     4621 accaaagagg ctacaaaagg aataatcaag gttggcatta aacctttaat ccagtttggt
     4681 actttgacct ttttaataat cgcttgaacc aagaaaccag ctatataacc tcccaagata
     4741 ccaccgataa accctgaacc aacagaattg gcaattagac caacaacaaa acctggtgca
     4801 atccctggtt taccagcaat cgagtaagac aaacctgtag caataacaac tggcaagaga
     4861 ccaagggctt taccacccat agttgctaaa gcatcccaga tagtgaattt tcctgctaca
     4921 agagcgtcag gaacaccacc ccccattgct aaaccaatgg caactaagaa tcctgcgcca
     4981 caaacaattg gaatcatata ggaaatggct gtcaataagt gaccttttaa gtttaagtgt
     5041 ttcaacatat attttctcct taaatatttt cagttatttc gtaacaatct caacagcttt
     5101 agcaatcagt ttattgggag atttgactgc cacttctgtt ggaaccttga taatcttttt
     5161 accctcaaag cgttccatac cagaaatctt aacatcaact gctaaaataa ctacatccgc
     5221 tgcatcaatc tgctcttgac tcaattcatt ttctacccct attgtcccct gagtctcaac
     5281 atgaatcaca tgtccagcta cctttgcggc attctctaat ttttcctgtg caatataagt
     5341 gtgggcaatt cccacagtac aagctgcaac accaacaatt ttcatacgga taccctccaa
     5401 aattttttct tattaacaaa aagctgcaat cacatcatca gatgtctgag cccgaactaa
     5461 tttggcaaca acttcgtcat taccaagttt tctagcaaag agtgataagg tcttcaaatg
     5521 ctccctagca gcttctgtat catcaccaac tgcaaagagt acaattactt tgaccccttt
     5581 cccatcaatg gtctcccaag gaatctcatt gtgatttata gctatgacta cccccgcctt
     5641 ctccacagca gaactcttgc tatggggaat agcaatataa ttcccaatac cagtctgtcc
     5701 ttctgcctct ctctgataaa gaccttcgat aaattggtct ctatcagaca cataacccgt
     5761 ctcaaccaat agttgagcta atgcctcaaa aacctcttct ttgctctgca tctgtaaatc
     5821 cgtctggatc agactcacat taagaatatc tttgatttcc atatattatc tcccgtaatt
     5881 cttcttttgt taactgtttt aattgattta tgaatgattc atctgctagt cttctcatca
     5941 atgttttaat acatgacttg tcctgtgata ctgcaatggc caaaccgata ataaggtcaa
     6001 cacactggat atccttcgac cattctctga taggtggttt taatctagta atcactaaga
     6061 catgatgttg aaagtttcct tcacaatgtg gtagaagaac acctttagca acctctatac
     6121 ttccctgtct ctcacggtaa tatagaagct cttctatttt ttctgtatct tcagaaacaa
     6181 gaaggctgat ttgatttgct aattctttgt aggcttcttg acgattttga acagatatat
     6241 ccataaggac aagcgaaaga ttattcatag tttatctcct gaatttttgc ttgaagacgt
     6301 tgtttatcac cctcggttag aaaaacacta actaggacaa acgggacact tgctggttcc
     6361 tgcaaagcta ccgtcgtcac aatgaaatct aaatctggat atagatttat cagctcatct
     6421 aattgatgat aagcaactac atcaataata tccaattcag taaattgctt ttctaatcgt
     6481 gctcgtaaaa gctctgaagt tccgacacct gatgtacaca tcactactgt ttttagagga
     6541 cgtgctcgct tttcttgaaa ccgtgcaaaa tataagacta gaaaaccaat ctcgtccaga
     6601 ctcaaggacg caaaaccaaa tactagactc acttctttag aaatagttgt taactcttta
     6661 accagattag gataggttaa aagaatttgt gataaaagac tattcttaat ctgtactcta
     6721 ttatctaatc tcctcagtaa gggactgata tgacttgcca agtcaacaaa gtcaggatct
     6781 gtcgtttcaa tctctctatt gtccatacac atacggctaa aatagtaatg agtgacatct
     6841 aaaactcgct gagaaaaagg aagcttcccg gaagaaaatg gtttttgcaa tctcgaagat
     6901 acaacgtatt gataaagata gtcaatctca actgcatcaa ccttcatcct aaaatattgt
     6961 tcaatttctt gaataatttt ttgacagaca ctgtaaattt tctcatcaac aatagtaggt
     7021 tttgaaggtg ctacaacatg aatactagta ctacgacgat tcctactgat aaaaatatac
     7081 aggtgagaga aaatatttat attatatgga tagggaatgt ttacccccaa ttctctctca
     7141 agtgtaatca gtaatcccaa acaaaagtga gcaagttctc cgtcaagagg ctgaacctta
     7201 ttctgtgtaa tttgctctaa atccaactga ttaaacattg gcagtagatt tagaatggct
     7261 gaacgaattt gagcctcatc cccatcaata aaaagctttc gttgtctcat ttttaaatct
     7321 aacccataaa ttgctagact ctcttgtaat atctgacgat cttttagtac tactgactcg
     7381 cttacgtaga attcctctcc taaatcataa atagaatgtg gcttaggagc agtcaacaag
     7441 agacgttcta gtaatttttc acgccttact tcaggatcaa aagcctctgt aaaattccca
     7501 tcaacgtcca cgagggaact tcttggattt aatttgaaac ctctgccttt ttcagataaa
     7561 atgaatgctt ctaccggaca atctttgttg attcccttga caaggcgata aaccgtttta
     7621 tcagagcaat ttaatttttc tcccaatact ttagcagata tataatcctt actatctcgc
     7681 aaaaggagca acaacgtttc catttgttct tgttttctag acacgataca cctcctttat
     7741 gattccatta tatcgctttc aaaaaataat atacttcgca ttttgtcctt tgcaatggac
     7801 atttttgttt ttatcaaaaa tatgatatca aatagaacag aacctcacta tgccaaaatc
     7861 attttacctt ttcttcataa catctatttt agttgaaata tttaagttgc ttttcgtatt
     7921 tgataagaaa taaaccaaac gattttctaa taaataacac tatgttccat ttaacaatag
     7981 attgcatctt cattttcaaa ctaccaacct atccgaccat ttgttctagc aatagaagag
     8041 agttaaaatt acttcaatct gtcgctttct ctttcttaca tcaaacttca aatagtctaa
     8101 ctacagatat ttgaatctat tacctcaatc caagcaggct tgagaatcac tcttgggttc
     8161 accgatagat aatagacacg taggccttgc acatcagatc tacgatagat ccatcgtata
     8221 aaaaactgca atccacagat tgcagccttt ctttattttc tggcttgata gcgactggtc
     8281 aggatgaaaa tcacggtaaa gaccaacatc atgacaccat aaacaggtaa tgtttgtcct
     8341 gttaaggttg caatttcaag cagtttttga attcttggga agagagctgt ggctaggaag
     8401 cctcctactg accagacaat caataggact cgccatagag tgaatggcat gcaggctcta
     8461 aatacggata agaaaccaat tgaccccaag agataataga ggagagttga gatttctaac
     8521 tcagaccaac cttgactcgt tccaaagatt ttcacaaaaa gaacgctaaa cacgaccatg
     8581 agggcacttg gtagggcacg aagcatggat cttctgagga agtttggctc gacaggtttg
     8641 atatttcgct caaaagtcaa aacgaatggt gggaaacctt ccacaaactg gtcaatcatg
     8701 gtaatctgga ttggaatgaa ggggaaaatc aagatccatt cagaccgacc tagtaaagca
     8761 ctggcgatgc agataactgc gagcaagaat gagtagatgg tctttatcaa gaaaatcgga
     8821 gcgatgtggg caatgttatt gaccacacga cgaccttcaa aaagaatctc aggaacatca
     8881 ttaaagtctg agttcaagag aaccaggttg gcaatctgac gggttgccgg gtctccctcc
     8941 gccattacga tagaacaatc cgcctcacga agagccagga tatcattaac tccatcccca
     9001 gtcatagctg ttgtatgacc cgcttttttc agcgtttgga taatgagttt cttttgatga
     9061 ggggaaacac gtccgaaaat cgctgtctcc tccgccagag caatcaattc ctcatccgtg
     9121 atttttgaac aatcgacata gctgtgatag tccgcaaaac cagccttctg ggcaatgctg
     9181 gataccgtaa ctggattgtc accagagata atcttgagcc ccacttcctg agaacggaga
     9241 tagttcagcg tctctgccgc tccctctcga ataggatcca agatttccag caaggctaga
     9301 gcctgaatat cagatggttt ctgtggtttg tgatggtcta atttctcctg actgagagct
     9361 aagaccaaga cacgtgaacc tctctccaag gcctctctgg cttctgggac ctcagaatcc
     9421 agcaacatct caggtgcccc taagaaaact attcccaaac cttctaactc catggctccc
     9481 cacttgcggt cgctggagaa gggaagatta gagagcatag gatagactac ttgaccttgg
     9541 aatctctggc gaatggcgtg agctgttgga tttttatcct cactatgggc catatagctg
     9601 gtcaggatgc tggcaacagc ctcttcacca taagtttccg tcagtggaag aacagcctcc
     9661 acctgcatct ttccttgggt gatggtaccc gtcttgtcca gacagagcat atccacgcgc
     9721 gccaaggtct caacagagta catctcctgc accaagacct ttttcaagcc aagcttaatc
     9781 actgcagtca agagcgaagt aatggtcaaa agggcaattc ccttaggcaa cattcccaaa
     9841 agggctgtcg acgaatttac aacggatgac ttgagaggca ggccttttaa aagcaaggct
     9901 tccagcaaga gagctagacc aaagggaatg ataatcttcc cagtaaaacc agccaacttg
     9961 tccagcgatt tcatgatacg ggagttgatg ggtttaacgg tcttagcctc aagcatgagt
    10021 tttgcagcat agttgtctgc accgacatgg tgaacttgag ataaaactga cccactggct
    10081 aggaaacttc ctgacaaaag taagccgtca acttcctttt gcaccaaatc actttctccc
    10141 gttaacatgg cttcattgac ttccgcaaag ccttccaaaa ccaaggcatc actaggaatc
    10201 tgctctcctg cagacaaacg aatgacatct cctagcacta attcttcagg attaagagca
    10261 acttcctgac catcacggat ggttttgacc ttttccttgg tcatgagatt gagcttgtcc
    10321 accatgtgtt tggctcgtag ctcggtcaca atcccagaaa aagcgttaaa gcagataaca
    10381 gcaaagaaga ccagattgct ccaagcctgc acaaaggcaa gggccaaagc aatggcaaag
    10441 ttcaaagcgt taaaaagggt aaagacattt cgtttaacga tttgccaagt gctggtactg
    10501 gctgatgcgg taaagtcatt gaccaagccc tcagcctgtc tttccttgac ttctctttgg
    10561 gttaatccca taatcttatt tttatccata aattctatta tatcattttt tttaacagaa
    10621 ttctaatctc atcccaaata tgcatctagt caagggaaaa gtttgccagc cctcctttga
    10681 taaggtataa taataacaag aaaacaatcg aaggagatcg catgttttat acttatttgc
    10741 gtggattagt tgtattgctc ttatggtcca tcaatggcaa tgctcactat cataatactg
    10801 ataaaattcc taatcaagat gaaaattata ttttagttgc gcctcaccgt acctggtggg
    10861 atcctgttta tatggccttt gcgaccaagc caaaacagtt catctttatg gcaaaaaaag
    10921 aactctttac caaccgtatc tttggttggt ggattcgtat gtgtggcgcc tttcccatca
    10981 accgtgaaaa tcccagcgcc tcagccatca aatatcctat caacgttctc aaaaaaagtg
    11041 accgctctct catcatgttt ccaagtggta gccgccactc aaacgatgtc aaggggggcg
    11101 cagcactgat tgccaaaatg gccaaggtcc gtatcatgcc ggttacctac gccggtccca
    11161 tgactttgaa gggcttgatt agccgtgaac gtgtcgatat gaactttgga aatccaatcg
    11221 atatctcaga tatcaagaaa atgaatgatg aaggcattga aacagtcgcc aatcgtattc
    11281 aaacagaatt ccaacgtctg gacgaagaaa cgaaacaatg gcacaatgat aaaaaaccaa
    11341 atccactctg gtggtttatc cgcatccctg ccctcatcct tgctattatc ctcgctatcc
    11401 taaccatcat ctttagcttt atcgcaagct tcatctggaa cccagataag aaaagagaag
    11461 aacttgcata gaagaaatga accttggcca aacagctaag gttttcattt atatagtaga
    11521 ttggaactag aatagtacac ctctacttct aaaacatttt tagaaatcga tttgactgtc
    11581 ctgatcgatt ttgtcctatt cttatttcat tttactatat ttataagaat ctcctaatca
    11641 aatcaaaagc cactctacaa tagagtaact ttaagattgt ccatttatac tcaatgaaaa
    11701 agccctctga aaatcagaga gctgatttga gctcaggcta aaatcctagt gaaacaaaaa
    11761 aatctacacg atcagaaaag tctctatcgt gccattttca tacatgatgt atagtccaag
    11821 tagaatgaat actaagggca caatgattcg ctcgtatttt tcaattgtct cgaatattaa
    11881 cggaatagag gataacaccc tactaatctc gcaaaagata attatgccga ttacaaacac
    11941 aagcaaggcc acgagggtct gtgaccaatc taacgaagca aaataaggta tatagatacc
    12001 taaattatct ccgccagacg caattgtcag caatgtaact gtccaaaaca gttgatttgc
    12061 cttgctttgt tctaatcttt caataatttc ttcctcttct tcttccgcat cttctccaac
    12121 aattgcaaag cgaatcccta aatagatagg gattaaacca agcaatccaa ccatccattc
    12181 ttcaggcacg aaattaacga cataagcagc aactaaactc gcccctacaa gtaagcctgt
    12241 gcctagatat tgccccgcat aaatatgcca tttctgttta ttctgtgata gctgtgcaaa
    12301 taaaataatt aaaataatta aataatcgat actggtggaa atataaacac caatagcaga
    12361 tatgattgtc tgtcccataa aaaaaacctc ctgtattagt cagtaataaa tcagcagatt
    12421 ttaggagagc acagacacat taatttagct atcgctagtg ggtaatgtcg aacgtaggaa
    12481 agcattttac tcctcctcaa aacgtagtta caaagtaatt tctaactgga attcagtgac
    12541 tacttccatg taaagtataa catactatac tttactttgt aaagagtggg ctcttctagg
    12601 gggcttgcat acagctactc ggcttgaaaa gccgagtaac tgcgcacctt tatggtgtaa
    12661 ttagctgata ccgtttgagg tttttgtagt ctccaaaatt gttaccgata aaacctcaat
    12721 aaaaaagccc tctgaagtca gagagctgat ttgagcctgg gctaaaatcc cagtgaaaaa
    12781 gatgaaactc cttgtgctca tcgaacactg tgtcatttcc ctattttcat acggattttt
    12841 gacgccctta gcatcatgat tcttgctaga taaatcgttt atagactagg cgacgaggcg
    12901 aagatcgtac gaaactttca gtgaaacaaa aaaaatctcc ccgaagggag aaaagctggt
    12961 ttattttacc ttacagtgct aggaaccgta accaaagatt atacttgagt atatcgaggt
    13021 aaggtgacaa cgcaaaaaag ctacccaatt tagagtagct tcattatcaa gatatgctca
    13081 attgaacacg gcctaagcac ttggcaaaaa agataaaatc tcctagaaac tgaagtttct
    13141 tcgtcaattt tcctattttt gctttgtgct tttgacagca tttgtatctt gaattagcga
    13201 cgaagtccta gagagttgat caactcacgg taacggttaa cgtcgttttt acgcaagtat
    13261 gcaagcaagt tacgacggcg accgattttt ttcatcaatc cacggtaagt agcgtggtct
    13321 tttttgtgtt gtttgatgtg ttcgttaagg tggttgattt cccaagtaag gacagcaact
    13381 tgaacctcta ctgaacctgt atcaccttcg tgacgtgcat attgtgcaat gatttcattt
    13441 tttttctctt ttgagattgc catgatgttt ctccttttta tttggcttca tccgagtgac
    13501 aggttggcat gcctgtaacc aagaagaagt tatttgtctt tacgacaatc cctattttac
    13561 caagatttaa attttctgtc aaacattaat actctcatag ttattatttt tcaagtaaag
    13621 agatacgttc ttttatatga tgaccttttt caagttcatc taccaatcca atagcataat
    13681 ctgcataact aatctggctt attccttttt cattagtagt aaaaatttct cctgctagaa
    13741 tgtagtttcc tgttttttca ccatcgggaa taaaatctac agcaggactg acaaaagtcc
    13801 agttgagatt attttttgta cgaagaagat ccaattcatc cgcttgatct tttgctagtg
    13861 acttaaattc ttcaggaaaa tcagacgtat ctaaaagtcg tgtcgttttt gtttcatcta
    13921 tataaagact tcctgctccc cctacaacta gaaaacgtgt ctgtgttcca gcaagaagct
    13981 gattaaatag ttcgattgat ttgctgtgga gcggtagcgt atctggtgta taagcaccaa
    14041 acgctgaaat aacagcatca aatccagtaa gatcatcttt tgtcaactca aataaatctt
    14101 ttttaataat agactcagct tgacttttgt tttcagaacg aacaatagcc gttacttcat
    14161 gtcctcgttt gactgcttct tcaacaattg ctttccccgc ttgtccattt gctgcaataa
    14221 ctgctagttt cattttttat acctctcttg ttgtaattat tttagttaca acaattgtga
    14281 cacttttaat aatcaatgtc aatagtcttg cttaattatt atcaaaatat ttctaccaag
    14341 aaaactaacc atgattctag tgaaaaaaat cttctttgtc aacaaattta ctttcttgtt
    14401 ttaaatatgc tataataatc atagcaagag atctaagttg tctgtttttt taaaacgagg
    14461 tgattatcat gcgtagattc tattcccatc tcccctacta tctggtcata ttattctttt
    14521 attggccact ttatgagttg ttcttgctag ttgtttctga cccccttaca ctatataaac
    14581 aatcttctct tctttacacc tctggtaatc ttgattgtat cgttactcta tagctaccgt
    14641 ttccgtttct cactttgatg gttagttggt aacggactgc tcttttactt tactatcata
    14701 acctttggtg agtttatact aatttacttg ctaatctatg aaacagttgc tctggtcggc
    14761 atggattctg gtattagcat caagcatatt ctacaaaaaa tgaaaaacaa aaaactttca
    14821 caaaatcctt gaaaaatctc acaatcatgc tataataatc catagagaca agtcacttag
    14881 tccctttcta ctagagagtg cgtggttgct ggaaacgcat aggaagtcta aactgatact
    14941 actcttgagt tttttatgaa aacataaaac ggtggccacg ttagagccga tcagaggtgt
    15001 ccctctcttt tgaggtacat aaatgaaggt ggaaccacgt tgcgacgtcc tttcgaggat
    15061 gtcgcatttt tttattagga tactaattat ggagttgcaa gaattagtgg agcgcagttg
    15121 ggcaatccga caagcttatc acgaactgga agttaagcat catgatttca agtggacggt
    15181 agaagaagac ctcttggctt tatctaatga tattggaaat ttccaatgac tggtgatgac
    15241 aaagcaagga cgctactatg atgaaacacc ctacacactg gaacaaaaac tttcagaaaa
    15301 tatctggtgg ctattagaac tttctcaacg tttggatata gacattctga cggaaatgga
    15361 aaacttcctc tctgataaag aaaagcaatt gaacgttagg actcggaagt agtctgctga
    15421 taaaaaatca atgcttagaa actatgaaat aataaaaaag gagaacatca tgattaacat
    15481 tactttccca gatggcgctg ttcgtgaatt cgaatctggc gtaacaactt ttgaaattgc
    15541 ccaatctatc agcaattccc tagctaaaaa agccttggct ggtaaattca acggcaaact
    15601 catcgacact actcgcgcta tcactgaaga tggaagcatc gaaattgtga cacctgatca
    15661 cgaagatgcc cttccaatct tgcgtcactc agcagctcac ttgttcgccc aagcagctcg
    15721 tcgtcttttc ccagacattc acttgggagt tggtccagcc atcgaagatg gtttctacta
    15781 cgatactgac aacacagctg gtcaaatctc taacgaagac cttcctcgta tcgaagaaga
    15841 aatgcaaaaa atcgtcaaag aaaacttccc atctattcgt gaagaagtga ctaaagacga
    15901 ggcacatgaa atcttcaaaa atgaccctta caagttggaa ttgattgaag aacactcaga
    15961 agacgaaggc ggtttgacta tctatcgtca gggtgaatat gtagacctct gccgtggacc
    16021 tcacgttcca tcaacaggtc gtatccaaat cttccacctt ctccatgtag ctggtgcgta
    16081 ctggcgtgga aacagcgaca acgctatgat gcaacgtatc tacggtacag cttggtttga
    16141 caagaaagac ttgaaaaact accttcaaat gcgtgaagaa gctaaggaac gtgaccaccg
    16201 taaacttggt aaagagcttg acctctttat gatttcacaa gaagtgggac aaggtttgcc
    16261 attctggtta ccaaatggtg cgactatccg tcgtgaattg gaacgctaca tcgtaaacaa
    16321 agagttggct tctggctacc aacacgtcta cactccacca cttgcttctg ttgagcttta
    16381 caagacttct ggtcactggg atcattacca agaagacatg ttcccaacca tggacatggg
    16441 tgacggggaa gaatttgtcc ttcgtccaat gaactgtccg caccacatcc aagtttttaa
    16501 acaccatgtt cactcttacc gtgaattgcc aatccgtatc gctgaaatcg gtatgatgca
    16561 ccgttacgaa aaatctggtg ccctcactgg ccttcaacgt gtacgtgaaa tgtcactcaa
    16621 cgacggtcac ctattcgtta ctccagaaca aatccaagaa gaattccaac gtgcccttca
    16681 gttgattatc gatgtttatg aagacttcaa cttgactgac taccgcttcc gcctctctct
    16741 tcgtgaccct caagatactc ataagtactt tgataacgat gagatgtggg aaaatgccca
    16801 aaccatgctt cgtgcagctc ttgatgaaat gggcgtggac tactttgaag ccgaaggtga
    16861 agcagccttc tacggaccaa aattggatat ccagattaaa actgcccttg gaaaagaaga
    16921 aaccctttct actatccaac ttgatttctt gttgccagaa cgcttcgacc tcaaatacat
    16981 cggagctgat ggcgaagatc accgtccagt catgatccac cgtggggtta tctcaactat
    17041 ggaacgcttc acagctatct tgattgagaa ctacaagggg gccttcccaa catggctggc
    17101 accacaccaa gtaaccctca tcccagtatc taacgaaaaa cacgtggact acgcttggga
    17161 agtggccaag aaactccgtg accgcggtgt ccgtgcagac gtagatgagc gcaatgaaaa
    17221 aatgcagttc aagattcgtg cttcacaaac cagcaagatt ccttaccaat taattgttgg
    17281 agacaaagaa atggaagacg aaacagtcaa cgttcgtcgc tacggccaaa aagaaacaca
    17341 aactgtctca gttgataatt ttgttcaagc tatcctagct gatatcgcca acaaatcacg
    17401 cgttgagaaa taagagtcta gcataaaagc ctccaatctg gaggcttttt ctcatctatt
    17461 tttactcaag gactaagttc acttgagcaa actgaatccg cactgtcgtt ccttttccga
    17521 cctcagactc gatacgaatc tggtgcccca gttcttcaga aattttctta gatagataaa
    17581 ggccaagtcc agaggactgc tgggtcaaac ggccattgta tcctgaaaag ccacgttcaa
    17641 atactcggag gacatcactg ttttttatcc cgattcccgt atctttgata caaagctctt
    17701 ggtcatccat ataaatctcc agaccacctt ccttggtgta cttgagactg tttgagatga
    17761 tttgctcaat aaccactagc agccactttt tatccgtcac gatttcttta tcaaggtcat
    17821 gtagattgac atttaagcct ttttgaataa agaaaagagc atatttacga attatttcct
    17881 tgaccaagtc ctcaatttga acctgcttta agagcaaatc atcatggaaa ctttctaaac
    17941 gcaggtactg taaaactagg ttggtatagg agtcgatttt gaaaatttcc tgttctagct
    18001 gctgcttcag ttggcggtcg accacttctg caactaagag ttgactggct gcaatggggg
    18061 tctttatctg atggacccac aaggtatagt aatccagcaa atccgtcagt tttctttctg
    18121 cttttgacct ctgctgatag agttccatct cacgcgcttc taatttttct gctaaagcta
    18181 tttccaaagg agacttggct tccctctctc catagagaag ttcctggcga tagacctgcg
    18241 tttccaccaa tatgtcccaa gtgaaaaata agatggttac aaagcaacac aagaagaaaa
    18301 agtagaggaa gtaaattcct agactggcaa ataaaaactg aaagagtaag acaagaaatg
    18361 ccaaagaaag cagatagaca aaaagacgac tacgggagcg cagataggct agaaaaaatt
    18421 gtttccaatc aagcatgctt caatccgtac cctattcctt tcttggtctc gataaatcct
    18481 accaatccct gctcctccaa ctttttacgc aaacgagcca cattgacaga gagggtatta
    18541 tcatcaatga aaaagtcact gttccaaagt tcccgcatca ggttgtcacg tgctacgatg
    18601 ttgcctgcat gctcaaataa cacgcgtaaa atctggaatt cattcttggt caaattcaag
    18661 acttgccctt gataatgtaa atccatggat ttggtattga ggataacacc agcatattcc
    18721 agcaaactct catcacgccc aaactcatag gaacgacgca acaagccctg aaccttggct
    18781 aaaagaacct gctggtcaaa aggcttggtc acaaagtcat ctgcccccat attgattgcc
    18841 atgacaatat ccatagcctg gtctctcgaa gaaagaaaca tgataggtac cttggaaatc
    18901 ttgcggattt cctgacacca gtgataacca ttaaacaagg gcaaaccaat atccatgagg
    18961 accagatgag gttccgactg aacaaataga ctcaaaactt ccataaagtc ttctaccagg
    19021 accacttcaa atccccattc agagagcatt ttcccgacct gttgacgaat gacctgatca
    19081 tcttccacta ataaaatctt gtgcatgcgc ttctcctttt ctactattat aacagatttt
    19141 tccatgctag atggtctgaa actgaatttg aaatagcctg tttttagcca gtacaaacag
    19201 gcaatgctac tagctaattt gagggaaatt tgctaagata aataaaaaga aaggagctct
    19261 tatggccaat atttttgact atctgaaaga tgtcgcatat gattcttatt acgaccttcc
    19321 cttgaatgag ttagacattc taaccttaat agaaatcacc tacctctcct ttgataatct
    19381 ggtctccaca cttcctcaac gtcttttaga tctagcacct caggttccaa gagatcccac
    19441 catgcttact agcaaaaatc gccttcaatt attagatgaa ttggctcaac acaagcgctt
    19501 caaaaattgc aaactctccc attttatcaa cgacatcgac cctgaactgc aaaagcaatt
    19561 tgcggctatg acttatcgtg tcagcctcga tacctatctg attgtctttc gtgggacaga
    19621 tgacagtatc attggctgga aggaagattt ccacctgacc tatatgaagg aaattcctgc
    19681 tcaaaagcac gcccttcgct atttaaagaa cttttttgcc catcatccta agcaaaaggt
    19741 tattctagct gggcattcca agggaggaaa tctcgctatc tatgctgcta gccaaattga
    19801 gcaaagtttg caaaatcaga tcacagcagt ttatacattt gatgcacctg gtctccatca
    19861 agaattgaca cagactgcgg gttatcaaag gataatggat agaagcaaga tattcattcc
    19921 acaaggttcc attatcggta tgatgctgga aattcctgct caccaaatca tcgttcagag
    19981 tactgccctg ggtggcatcg cccagcacga tacctttagt tggcagattg aggataagca
    20041 cttcgtccaa ctggataaga ccaacagtga tagccagcaa gtagacacaa cctttaaaga
    20101 atgggtggcc acagtccctg acgaagaact tcagctctac ttcgacctct tctttggcac
    20161 tattcttgag gctggtatta gctctatcaa tgacttggct tccttaaagg cgcttgaata
    20221 cattcatcat ctctttgtcc aagctcaatc cctcactcca gaagaaagag aaaccttggg
    20281 tcgccttacc cagttattga ttgatactcg ttaccaggca tggaaaaata gataatactc
    20341 ttgaaaatta aatgtataca aaacaaaaga cctagaatac atactttcat gtgcattcta
    20401 agtcttttta aatagaatct aatagtcaat aaaaatcaaa tagcattgag agataatggg
    20461 gcttggaacg tccctctcgc ttcaacaaaa tgaccccatt atagattaaa aagatgccac
    20521 ttagaaaaag caaaaaagga agtaagacaa aggcaaatac ataaaaagct aactgaacat
    20581 tctcgtatcc atttttataa aaaaggtagg atagataaaa ataacttgaa atgagggata
    20641 ataaaaataa tactggattc cacaaacttc tattatcctt ccaaaatgac actataaagg
    20701 ctaatacaat tcctataacg agatacattt cttactcctt taatagctac attttatcat
    20761 aattatccaa agaaaaaaga gggcatttat ccctcttaat ccttcatctg actctctgca
    20821 tcggccacga ctttttctag actggtttga ccaagttctg cctccatagt caactgaatt
    20881 ctctccaatt tttgatccaa aacatcatga atatgagctc ctacagggca atttggattc
    20941 ggattgtcat ggaaactgaa gagttgacct gtcttaccaa gacattcgac cgcctgataa
    21001 acatctaaaa gactaatatc cttaaggtcc ttgacaatct ctgttccgcc cgttccacgc
    21061 gctactgaaa tcagctctgc cttcttcaac tgggacaaga tctttctgat aatgacagga
    21121 ttgaccccga cactagcagc cagaaaatca ctggtcacct tgctttcctt cccctcgagg
    21181 gcaatgatta tcagcatatg agtcgcaatg gtaaatctac ttggaatttg catcctcttc
    21241 tcctttttac gaggctaccc tgcctctact cttctttttc tattattata ccctttttag
    21301 ttgtaatgtc aatcgttacc acttttcaac cagtcgtcta actcccggtc gcagccctct
    21361 ttctgagcca attctctcaa aaattcctga tgatgagtat ggtgaatccc attgaccaga
    21421 ctttcatagt aaacctcaaa atagggaagt ctcaggtctt tagccagctg caattccgct
    21481 gccacatcgt agtctacccg tcggaagtcc atatctacca ggcctttgtc atcaaactcc
    21541 aaaatcatat actgggcccg caagtccttc cgtagctgag cgtccaaaaa gaaaggttgg
    21601 ccaatcgaac ccggattgac aatcaattgc ccaccagtcc cgtaacgaag caactgctgg
    21661 tgaatatgtc cataaacagc aatatcacag ggaggatgag tcaccaagcg gtcaaactcc
    21721 tcttgtttgc cagtatgaat caactctcgc ccccagttct tatcaggcag atgatggcta
    21781 attcccaccg tcaaatcccc aaactgacga tgaatttgaa gaggttgatt gtggagcact
    21841 tcaatttctt ctagggaaat ttcctctaaa acatactggc actggcgcaa gagatagcgt
    21901 tgactggggc gagtactgtc caattcctta cggacaccat gccaaagact gtcttcccag
    21961 tttcccaaaa ctctagccgt aatcggtagt tgatccaaca agtccaaaat ccttctacgt
    22021 cctgtccctg gcatgagaat atctcccaaa agccagtatt catccactcc tagctgctga
    22081 gcatctgcca aaacagcctc caaggcggtg gtatttccat gaatatctga aagaagagct
    22141 attttcgtca tatccatctc ctcgtttttt ctcttgcaat aagtataaca taaaaagtca
    22201 cagctagaga aatctagctt tttttgatat actagataaa gatattagac aagaggaaac
    22261 gaatgacccc aaacaaagaa gactatctaa aatgtattta tgaaattggc atagacctgc
    22321 ataagattac caacaaggaa attgcggctc gcatgcaagt ctctccccct gccgtaactg
    22381 aaatgatcaa acgaatgaaa agtgaaaatc tcatcctaaa ggacaaggaa tgtggctatc
    22441 tactgactga cctcggtctc aaactggtct ctgagctcta tcgtaagcac cgcttgattg
    22501 aagtttttct agttcatcat ttagactata caagtgacca gattcacgag gaagctgagg
    22561 tcttggaaca cactgtctct gacctgttcg tggaaagact agataaactg ctaggtttcc
    22621 ctaaaacctg cccccacgga ggaactattc ctgccaaggg agaactactc gttgaaatca
    22681 ataacctccc actagctgat atcaaggaag ctggcgccta ccgcctgact cgggtgcacg
    22741 atagttttga cattctccat tatctggaca agcactcact tcacatcggt gaccagctcc
    22801 aagtcaagca gtttgatggc ttcagcaata ccttcactat cctcagtaac gacgaggatt
    22861 tacaagtgaa tatggacatt gcaaaacaac tctatgtcga gaaaatcaac taatttctca
    22921 agtcccctac caaccctgaa agttttattt tcagggtttt atttctatca ttttatttta
    22981 ctttcatttg gtggtagaat atttgaaaaa aaaaagaaag gtagtttaac ctatgaaaaa
    23041 atcactaaaa atttttgcta catctaaatg gtttgacctc ttcggggttg ctttggtcgt
    23101 tgggattgcg attgcatctg gttacctcaa ctcacgcctc gataaatttg tagactgggg
    23161 accatggaca gctcttgtac cctttggatt gatttccgta accaacgttg ggatttccat
    23221 gttgtccact cgtttcacgg ggaaattaag caaatgggga aattacttta gtattgttaa
    23281 taccattttg tccggtgcta ttgattatat ccttggaaat aaggcggcca tcattaccta
    23341 tcccgtcacc ttcctcattt atacctttgc tataaagaaa tggaaagctt cgcaagaagg
    23401 cagacccaac caaatgagcc aaaaacaggt aaaattggcg gtcatcatca tttccatcat
    23461 cgccttcctc tttgcctttg tgaccaacta tatcggatat gagggcaaga tgaatctcct
    23521 tgcctacgta acaactattg cctttgcact atccctcatt gccaatgctt tgaatgcatt
    23581 aaaactaaca actcagtggg gcttttggtt gatttacaat ttcgttcagc tgacaaaagc
    23641 tggtattcaa ggaaacttcg ccaatatcgg taaatacatc ttttatatcc tcaatgcaat
    23701 cggagcttta tttgtctgga atgatgaaga agttagataa tagaaggaaa ctcaaataaa
    23761 gaatactagc caacttaaga gaatctccct tctgctattt ttatcatttt agttgtaact
    23821 cccattcttt tggagtagaa tgaagttaga taaaagaggg aggtcttgtc atgaaaaaac
    23881 tctatagaat ccattttata gcaattgcag ttattgattt gctactattt gcatttttta
    23941 tcattagact cgagacatct tttgagtggc tcttgctctc tggtttaatt ttcttccttg
    24001 ctcaaggact cctgctattt ctcttgatcg tccgactcaa acatcaattc gctgagattt
    24061 atcctcaaat caataaaaag attcgcttct actatttagg ggttctcacc attgattttc
    24121 tattttttgt tctcttagcc ttcattagtt ctcagcgttt ttcatctctt atgccaatca
    24181 tcactgcttg ccattctact ttttattata tgacagctga ctacctaaga gaaaactatc
    24241 cagactttta cgacaaacac atctctttat gggagtgtct ctaaggaaaa ggaggtttta
    24301 gcatgaaaaa aatcatcttc atcaaaacca ttcaactcct tgtcattgat ggaatcatgc
    24361 tggcattttt gacatttaaa aggggggctt acttgggact ggattttgat ttatagcggt
    24421 tggctcattt tctttcatcc tgtgctattg acctatcttt caaaccaact ttgtgaccac
    24481 tttagttaac tctattccca gattagaccg agattctggc gttttgcttt acaaattctc
    24541 ctatgggata gcctgatgat tctctccttg gtgtctttaa gtgatattcc acttttcctt
    24601 cagggaactc tcctcatcct aggacatctc atcccttcct atcgcatctg ccaaagcctg
    24661 aaaagagact tcccccaagc atatcaagaa ccgatttctt tttggagtat tttatgatag
    24721 atgagaaaga ccaagccgac tgggcttggt ctttcttatc tctttttagt atctaggata
    24781 atggtaacag gtccgttatt aaccagctca acctgcatat ctgctccaaa gatgcctgtc
    24841 tgaacgggca cttcttgcgc taatttttga ttgaaagcat catagaagtc tgatgccata
    24901 tcaggtttag ctgcccctgt aaaggctgga cgattgcctt tcttagtatc cgcaaagagg
    24961 gtaaactgag aaatagagag gatttctcct tcaatatctt tgacagacag gttcatcttg
    25021 ccttctgcgt ctgaaaaaat ccgcatattg accagttttc tcacagcata gtccaaatct
    25081 tcctcttggt cctctggtcc aacaccaacc agcaataaaa gtccctgatt gatttttccc
    25141 tgaatctgac cttctatact cacttgggct tttttaaccc gttggataat gattttcata
    25201 atagcctttc tagtaagagc taggacaact agccgttggt ccgtttgaca gagtaaactt
    25261 ctggcacact cttaatttta tcgacaaccg tggtcagtgt agagaggttg gcaataccga
    25321 aggacacatg gatattagca aacttcatat ccttggttgg ttgggcattg accgttgaaa
    25381 tattcttggt tgtatttgaa agaacttgca gtacatcgtt caacagtcct gtacggttga
    25441 gaccgtagat atcgatatgg gccatatact ccttatttga gctagagtac tggtcttccc
    25501 attccacatc aaggagacgt tgctcgtagt tttcttgggc acgcaggttc atacagtcca
    25561 cacggtgaat agccacacca cgacccttgg taatgtagcc aacaatatcg tcaccaggca
    25621 cggggttaca acacttagca atccgcacta ggagaccaga agcaccttca ataaccactc
    25681 ccccctcatg cttgaccttg agagtttctt tattttcaac cttgacctcg ccacctttga
    25741 caagctcctc tgcctcagcc ttggccttgg cacgctcttc ctcacggcgt tccttttcag
    25801 tcagacggtt aaagacggta atcgcaccga tttccccaaa accaatggcc gcaaagaggg
    25861 agtcttctgt cttgtaactg gtcttttgca gaacttgatc catgtggcgc ttgtccataa
    25921 atttatttgc cacatagcca ttttcttgga actgagccat cagcatctca cgacccttgt
    25981 tgacagacaa ttccttatct tggtttttaa agaactggcg aatcttattg cgcgccttgc
    26041 tagtcttgac catattgagc cagtcacggc taggtccaaa ggagttcggg ttggcgataa
    26101 tttcaacctg atcccctgtc tttaacttgg ttgtcagtgg aaccatgcgg ccattgacct
    26161 tggcaccagt tgctttttca ccgaccttgg tatggatttc gtaggcaaaa tcaatcggtc
    26221 ctgaatcttt gggaagagaa cggacagctc catctggggt aaaaacgtaa atctcctcag
    26281 ccagatagtt ttccttaaca gagtccacaa attccttagc atcatcagcc tggtcttgga
    26341 gctccatcat ctccttgatc cagttcattc caatagctga ttccttgctg ttaacttgcc
    26401 cctttatacc tttcttataa gcccagtgag ccgcaacccc gtactcagcc acctcgtgca
    26461 tttccttggt tcgaatctgg aattcaatcg gcccttttgg tccataaaca gtcgtatgga
    26521 tagactgata accattggcc ttgcggttgg caatatagtc tttgaagcga cctggcatcg
    26581 gtttccaaaa ttcatgcacg taaccaagca tggcataaac atcactttgg gtatctaaaa
    26641 tacaacgaat agcaatcaga tcatagattt cctcaaaccg ttttctcttg tcctgcattt
    26701 tgcggaaaat tgagtaaata tgcttgggac gaccataaat cttccctttc aagtgacgtt
    26761 ctgtcgtata ctcctctaat tttgtgacta cctcatccac caaggcctca cgctccctgc
    26821 gcttttcctt catcatatgg gtaatcttgt aaaactccgt tggattgaga taacggaaag
    26881 acaagtcttc taattcccat ttgacactgg aaatccccaa acgatgggca agtggggcat
    26941 agatttccat ggtttctttg gaaatacgct cctgcttgtc ttttcgaaga tgtttcaggg
    27001 tccgcatatt gtgcaagcgg tcagacagtt tgaccaaaat aacgcggatg tcctcagaca
    27061 tggccatgag catcttgcga tgattttccg ctaattgctc ctcgatcgat ttgtactcga
    27121 ccttgccaag cttggtaact ccgtcaacaa tcatccgcac atcaggacca aactctcttt
    27181 ccaaatcgtc caaagtcgca tctgtatctt ccaccacatc atgcaagaat ccacaagcta
    27241 ctgttacagc atccagcttt agcttagcta aaatacctgc cacttggata gggtgaatga
    27301 tataaggctc gcctgatttg cgatattgac cactgtggca ttcaacagca tagaccaagg
    27361 ccttatggac aaaatgaaca tcctcttccg ttaaatattc tttggttaaa gcgacaactt
    27421 cttcgcctgt taaattcact tctttcggca tctctactct ccaattcttc ctaccatttt
    27481 atcacttttt taagaatatg aaaactagat tggaacagaa taagaaaaaa ataattcaaa
    27541 attgcttgat aattctgaat tattggccca taatatacta cgaagttaga ttttaaactt
    27601 aggtgataga aggagagata gaagaacgga aaccatattg taacccaaag actttctgac
    27661 ttccccaatt ccattgaaga tacgaaagat aaacggtgga actcgtatca catacactgg
    27721 taccttgact ggattttgga attaatactc aatgaaaatc aaagaacaaa ctaggaaact
    27781 agccgcaggt tgctcaaagc actgctttga ggttgtagat aagactgaca aagtcagtca
    27841 catatatacg gcaaggcgac gttgacgcgg tttgaagaga tttttgaaga gtataaaaat
    27901 cctcaagata ctttcttcta tcctttagtt tataaggaga atacctatga aaaaaactgc
    27961 tatttctatc tttgctctcc taatgttagg agtttgctgc ctgttcctat tcagccagca
    28021 aagctataaa aaactcgttc aatactatgc taacgaccag aacctgccca gtaggataac
    28081 ttatagtgaa tatagcgaca aatgagaagc caactacggt agcactctaa acatcacgtc
    28141 tatcaaacaa gctaatgacg gagtttatgc aacctatgaa gggaaattga cacctttcca
    28201 atattgataa attgataacc agcctgtctt catctagtca tgctggtttt taagttcatt
    28261 ttaaatcctt acctattctc cctaactgtg ctatacttaa tttatactca atgaaaatca
    28321 aagagcaaac tagaaagcta gccgcaggct gttcaaagca ctgctttgag gttgcagata
    28381 aagttgacgc ggtttgaaga gattttcgaa gagtattagt acattctttg agattggagc
    28441 tagtatgaaa atccataaaa ccgtgaatcc tgttgcctat gaaaatacct attatctaga
    28501 aggcgaaaag cacctcatcg tcgtcgatcc tggtagtcat tgggaagcca ttcgtcagac
    28561 aatcgagaag atcaacaaac cgatctgtgc tattctcttg acccacgccc attatgacca
    28621 tatcatgagt ctggacttgg ttcgcgagac gtttggcaat cctcctgtct atatcgcaga
    28681 gagcgaagcc agctggctct acactcctgt cgataatctc tccggtctcc ctcgccacga
    28741 tgatatggca gatgtggtca caaaacctgc agaacacacc tttgtctttc acgaagaata
    28801 ccaactagag gaatttcgtt ttaaagttct accgacccca gggcactcta tcggtggtgt
    28861 ttccctagtc tttcctgatg ctcacctagt cttgacggga gatgctctat tccgcgaaac
    28921 tatcggacgg accgaccttc cgactggtag catggagcaa ctccttcata gtatccagac
    28981 ccaactcttc accctaccaa actacgatgt ctatccagga catggtccag ctactactat
    29041 cgctcacgaa aaggccttca atcccttttt ctagcaagat gatgacaatc gaaatttaag
    29101 taaactatcc agcaaatctt tctattacaa aaggcatcct atcaaggttt tcacacgtga
    29161 ttggatgcct tttttctgat gactagattt tttgtattac caaataatca cgcgctcctc
    29221 tggtgaacgc cacattccgt ctccttcttt gacatcatag gttgtaaaga aatcgtcgaa
    29281 gtttggtact tgcacattga cacggagttt ggctggtgcg tgcacatcga cgctagccaa
    29341 aagtttcata aattctggtc gacctttcat gcgccagatg cgaccgaagt tgtagaagaa
    29401 ctcttctgct gagaagtctg cttctctctt agctgcttca agcgctgctg cgattcctcc
    29461 caagtcagcc acgttttctg atacagtcaa tttaccgtta atggttgctc cataagaatc
    29521 ctgtccatca aattggtcaa tgactttttg tgttttctcc ttgaaggcag catagtcgct
    29581 ctctgtccac caatccttga ggctaccatt ttcgtcaaag gaagctccgt tagtatcaaa
    29641 ggcgtgggaa atttcatggg caatcactgc cccaatacca ccgtagttag cagaagatga
    29701 ctgatgcaag tcatagaaag gcgcctgtaa aatggccgct ggaaagacaa tcaggttctt
    29761 ctgaggattg tagtaggcat tgaccatatg agcaggcatg ccccattcct tatagtctac
    29821 aggctggttc cacttactcc aactgtgctt gatttccaca cgcgcaaagg ctagagcatt
    29881 ctcaaaaaga ctggcagttt cattcactac cttatccttg taacgtgcag gcaattcttc
    29941 tggatagcca atataaggtt tgatcacatt gagcttcacg atagcctgtt tacaggtttc
    30001 tggagtgagc cagtcattct taagcagacg ctccttatag acatcaatca tggttgccac
    30061 ttttttctcc acatccgcct tggcttctgg agagaacttc tcacgggcgt accaaagacc
    30121 cagggcttgc ttgaaaggtt cttgtgctag atgataagct gctttgacct tatcttttgc
    30181 ctctggaact ccagaaaggg cacggctgta ggcaccagac aaaacacgga tatcctctgt
    30241 taaatagctg gttgaaagat tgacaacact caaaatcaag gttgctttaa ggagagacca
    30301 ggcttcctca ctgtagaatt gctctgccgc ttgccagaaa cgttcctcgt ctacaataac
    30361 cttgtctggt aattgcccaa taactgcttt gaagaagtca tccaaaggta gggcaggcgc
    30421 gaatttcttg aaatcttcgt aagaatatgg atgatagagt ttagcatatt ctgaactttc
    30481 ttcattagag agcaccactg ccgcaactcg gcggtccaat tcaagtcttt tttctagcaa
    30541 gtcttcaatt tcttcatcag agaaatcata agccttgagg agatttgcgc tgctttcttt
    30601 ccaaagagtc aagagctctt cgcgctgagg atgttcttct gcatagtagg tcgtatctgg
    30661 caagattgtg cttggagcgc tagcccatag aacattgatt ctagcatcca taaagtctgg
    30721 cgatacacca aaaggaagga agtttggttt tcctgcaagc tcaaactctg ctagtttagc
    30781 tgtaaaatcc gcaaaagtct ccaattcttg gaattcttta aggagtggta agacaggtgt
    30841 gataccgtca gcttctctct tgtcaaaatc acgaactagg cggtggtatt tgacaaagtt
    30901 ttccaagata gcatcctcag gcacttcttc acctgctaac cacttgtctg ttgtcgccag
    30961 catcaggtct tcaatttcct ggtctaaatc aacaaaacct cctgtttgag acttatctgc
    31021 tgggatttca gctgtctgtt gccattctcc attgatagca tcataaaaat catcttgata
    31081 acgtgtcatc ttgttctcgc tttcatttgt atttgcattt atcttaacaa aaatcactag
    31141 ggaatgcaat taataaccat ttaactatta ttt
//
LOCUS       DAVKQL010000025        30282 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVKQL010000025 DAVKQL010000000
VERSION     DAVKQL010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30282
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.025"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(14..655)
                     /gene="plsY"
                     /locus_tag="VW561_001034"
     CDS             complement(14..655)
                     /gene="plsY"
                     /locus_tag="VW561_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEU9943310.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            791..2734
                     /gene="parE"
                     /locus_tag="VW561_001035"
     CDS             791..2734
                     /gene="parE"
                     /locus_tag="VW561_001035"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEU9943311.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTNE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVVNTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            3154..5625
                     /gene="parC"
                     /locus_tag="VW561_001036"
     CDS             3154..5625
                     /gene="parC"
                     /locus_tag="VW561_001036"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEU9943312.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLV
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEAILYVEVLDQFDDATTYFAATRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            5755..6777
                     /locus_tag="VW561_001037"
     CDS             5755..6777
                     /locus_tag="VW561_001037"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEU9943313.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGIGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            6833..7537
                     /locus_tag="VW561_001038"
     CDS             6833..7537
                     /locus_tag="VW561_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9943314.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKYIK"
     gene            7602..8291
                     /locus_tag="VW561_001039"
     CDS             7602..8291
                     /locus_tag="VW561_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEU9943315.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            8288..9211
                     /locus_tag="VW561_001040"
     CDS             8288..9211
                     /locus_tag="VW561_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEU9943316.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            9226..9870
                     /gene="pcp"
                     /locus_tag="VW561_001041"
     CDS             9226..9870
                     /gene="pcp"
                     /locus_tag="VW561_001041"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU9943317.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            10038..10268
                     /locus_tag="VW561_001042"
     CDS             10038..10268
                     /locus_tag="VW561_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEU9943318.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            10318..10398
                     /locus_tag="VW561_001043"
     tRNA            10318..10398
                     /locus_tag="VW561_001043"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10352..10354,aa:Tyr,seq:gta)
     gene            10408..10479
                     /locus_tag="VW561_001044"
     tRNA            10408..10479
                     /locus_tag="VW561_001044"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10440..10442,aa:Gln,seq:ttg)
     gene            10641..11843
                     /gene="rpsA"
                     /locus_tag="VW561_001045"
     CDS             10641..11843
                     /gene="rpsA"
                     /locus_tag="VW561_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEU9943319.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPVQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            11929..12426
                     /locus_tag="VW561_001046"
     CDS             11929..12426
                     /locus_tag="VW561_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU9943320.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
     gene            12426..14081
                     /gene="dnaX"
                     /locus_tag="VW561_001047"
     CDS             12426..14081
                     /gene="dnaX"
                     /locus_tag="VW561_001047"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEU9943321.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIQEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNMFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNAGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            14109..14303
                     /locus_tag="VW561_001048"
     CDS             14109..14303
                     /locus_tag="VW561_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEU9943322.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            14453..15223
                     /gene="sufC"
                     /locus_tag="VW561_001049"
     CDS             14453..15223
                     /gene="sufC"
                     /locus_tag="VW561_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEU9943323.1"
                     /translation="MSVIEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELKVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            15251..16513
                     /gene="sufD"
                     /locus_tag="VW561_001050"
     CDS             15251..16513
                     /gene="sufD"
                     /locus_tag="VW561_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEU9943324.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            16524..17750
                     /locus_tag="VW561_001051"
     CDS             16524..17750
                     /locus_tag="VW561_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9943325.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKVINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHVSNVLSVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            17737..18177
                     /locus_tag="VW561_001052"
     CDS             17737..18177
                     /locus_tag="VW561_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEU9943326.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            18231..19643
                     /gene="sufB"
                     /locus_tag="VW561_001053"
     CDS             18231..19643
                     /gene="sufB"
                     /locus_tag="VW561_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEU9943327.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(19842..21083)
                     /locus_tag="VW561_001054"
     CDS             complement(19842..21083)
                     /locus_tag="VW561_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEU9943328.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            21186..22295
                     /locus_tag="VW561_001055"
     CDS             21186..22295
                     /locus_tag="VW561_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU9943329.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVIFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
     gene            22829..23176
                     /gene="ssrA"
                     /locus_tag="VW561_001056"
     tmRNA           22829..23176
                     /gene="ssrA"
                     /locus_tag="VW561_001056"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(23359..23446)
                     /locus_tag="VW561_001057"
     tRNA            complement(23359..23446)
                     /locus_tag="VW561_001057"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23410..23412),aa:Ser,seq:gga)
     gene            23609..24349
                     /locus_tag="VW561_001058"
     CDS             23609..24349
                     /locus_tag="VW561_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9943330.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            24346..25257
                     /gene="pfkB"
                     /locus_tag="VW561_001059"
     CDS             24346..25257
                     /gene="pfkB"
                     /locus_tag="VW561_001059"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEU9943331.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNISNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVEPVKLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            25254..27206
                     /locus_tag="VW561_001060"
     CDS             25254..27206
                     /locus_tag="VW561_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9943332.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIEEMIKSLTGHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALTELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLAISGDAEVYRAANGAK
                     VATASNEKQSLGGAFYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALSVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            27370..29595
                     /locus_tag="VW561_001061"
     CDS             27370..29595
                     /locus_tag="VW561_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU9943333.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSILKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPADMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDQDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     INENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEMAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(29792..30220)
                     /locus_tag="VW561_001062"
     CDS             complement(29792..30220)
                     /locus_tag="VW561_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943334.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTITTIGSILGLITAGITIMN
                     ALDNNNKNTKKEPTTSQTTTFVKEIQKESPQENTTPNKENNTSQEKTQQEETPKSSVK
                     EEKKEDQKTATQDSTTPATSKPATENEKQPNTPTSENNTQ"
BASE COUNT         9042 a         5501 c         6771 g         8968 t
ORIGIN      
        1 cagaactggc gttttatttt ttaggatctt gatgggttag gttcaatccc caagggacca
       61 aattttcagt tttatttttg atacgagcta tattgtcctt atgacgaatg ataatcaaac
      121 tagcaagtgc taagataata gcgatgaaga gagagtcata gttactcagg ataaaaccaa
      181 aaagtggaaa gagcagaacc ccgataacag ccgcgatcga tgctgtgaca ctagacagtg
      241 aaatcatact gccaagatag agagctccaa agaagataat cgcaaggtag agacagaaga
      301 taggcgcaaa tccgaaaatc actccagcac tggttgcgac agccttacca cctttaaatc
      361 ctgcaaagat agggaaggta tggccgataa cagccaaaag tccaaagatg agaggagaaa
      421 cgccttgtag atgaaaaata atcggaagca gcgttgctag ggttcctttg aaaaagtcaa
      481 tcacaaaggt tgccatacca gctttcttac ctaaaatgcg gaaggtattg gtcgttccag
      541 tgttaccaga accatgctcg cgtagattga tttgaaagaa tacttgtcca atccagagac
      601 cagatggaat cgaacccagc agataggcta ggattaataa aactattgta atcatactcc
      661 tattatatca tgaattggga aagaaaagca gagaatctct gctgaaattg tcacatcgga
      721 gaaagaaaaa ttttgcaaaa tccttggaaa acctgtagaa tagtaaagat gaacgaatag
      781 gaggttcctt gtgtcaaaaa aggaaatcaa tattaacaat tataatgatg atgctattca
      841 ggtgctagaa gggttggatg cggtccgaaa acgtccaggg atgtatattg gatcgaccga
      901 tggcgctggt cttcatcacc tagtttggga aatcgttgat aatgcagtcg atgaagcctt
      961 gtctgggttt ggtgatcgta ttgatgtaac tatcaataaa gacggtagtc taacggttca
     1021 agaccatgga cgtgggatgc cgacaggtat gcacgctatg ggaattccaa ctgttgaggt
     1081 tatctttacc attcttcatg ccggagggaa attcggtcaa ggtggctata agacatcagg
     1141 tggacttcac ggagtgggtt cttccgttgt taacgccctt tctagctggt tagaagttga
     1201 aattacccgt gatggcgcag tttacaagca acgtttcgaa aatggtggaa aacctgtcac
     1261 gactttgaag aaaatcggta cagcacccaa gtctaaaaca ggcaccaaag ttacttttat
     1321 gcctgacgcg actatatttt ctacgacaga tttcaagtac aataccattt cagagcgcct
     1381 taatgaatca gcctttctct tgaaaaatgt gaccttgtct ttaacggaca agcgaacaaa
     1441 tgaagcgatt gagttccact atgagaatgg agtacaagat tttgtttctt atctcaacga
     1501 agataaggaa atcttgacgc cagttcttta ctttgaaggg gaagacaatg gttttcaagt
     1561 ggaagtagcc ctccagtaca atgacggatt ctcagataac attctatcct ttgtcaataa
     1621 cgttcgcacc aaggacggtg gaacgcacga gacaggactc aagtctgcca ttaccaaggt
     1681 catgaatgac tatgcacgta aaacaggtct tctcaaggaa aaagataaaa accttgaagg
     1741 ttcagactat cgtgagggac tagcggccgt tctttctatc ttagttcctg aagaacactt
     1801 gcagtttgaa ggacagacca aggataaact aggaagcccc ctagctcgcc cagttgtgga
     1861 tggaatagtg gctgataagt tgaccttttt ccttatggaa aatggggaat tagcttctaa
     1921 cctcatccgc aaggctatca aggcccgtga tgctcgtgaa gcagcacgta aggcgcgtga
     1981 tgagagccga aatgggaaga aaaacaagaa agataagggc ttgttgtctg ggaaattgac
     2041 cccagcccaa tctaagaatc ctgctaagaa tgaactctat ctagttgagg gggactctgc
     2101 cggtggttct gccaaacaag gtcgtgaccg caagttccag gctattctac ctcttcgtgg
     2161 taaggttgtc aatacagcca aggccaagat ggcggatatc ctcaaaaatg aagagatcaa
     2221 taccatgatt tataccattg gtgcgggtgt tggagcagac ttctctattg aagatgccaa
     2281 ctatgataag atcattatca tgaccgatgc ggataccgac ggtgcccata tccagacctt
     2341 gctcttgaca tttttctacc gttacatgcg tccgctagtc gaggcaggtc atgtctatat
     2401 tgccctccca cctctttaca agatgtccaa aggtaaaggc aagaaagaag aagtggccta
     2461 cgcttggacg gacggagaac tagaagaact ccgtaaacag ttcggtaaag gcgctaccct
     2521 ccaacgatac aaaggacttg gtgagatgaa tgcggaccag ctctgggaaa caaccatgaa
     2581 cccagaaaca cgtaccctca tccgtgtcac aattgaagat ttagcgcgcg ccgaacgccg
     2641 cgtcaatgtt ctcatgggag ataaggtaga accacgccgt aaatggattg aagataatgt
     2701 caagtttacg ctagaagaag cgacagtgtt ttaatgaaag aaaataatat gttagtaaac
     2761 tggaaattag agtcggatgt gaatgactat gtgaaaaagc agtttgaaaa tcttggtttg
     2821 aaaaaattgc aggattatta atgaagaatc tgctatgagt gcttatctca aagaggcgtt
     2881 taagggagcg gctaagacac aaactaagac taattttgga aaaccatatt ttcatattga
     2941 aaaatacaaa caggatagtt tggttactta tcgtaactga acacgggacg aattcccgta
     3001 aaatatcttt tactgcataa aaacctactc tacattcttt gaaaggagtt gaacacgccc
     3061 tagatactgt gtgaaaaaga taaattctct tgtgagtttg cttacttcaa gaattttcta
     3121 ttttcacttg gtattttatg ggctttgtat cttatgtcta acattcaaaa catgtccctg
     3181 gaggacatca tgggagagcg ctttggtcgc tactccaagt acattattca agaccgggct
     3241 ttgccagata ttcgtgatgg gttgaagccg gttcagcgcc gtattcttta ttctatgaat
     3301 aaggatagca atacttttga caagagctac cgtaagtcgg ccaagtcagt cgggaacatc
     3361 atggggaatt tccacccaca cggggattct tctatctatg atgccatggt tcgtatgtca
     3421 cagaactgga aaaatcgtga gattctagtt gaaatgcacg gtaataacgg ttctatggac
     3481 ggagatcctc ctgcggctat gcgttatact gaggcacgtt tgtctgaaat tgcaggctac
     3541 cttcttcagg atatcgagaa aaagacagtt ccttttgcat ggaactttga cgatacggag
     3601 aaagaaccaa cggtcttgcc agcagccttt ccaaacctct tggtcaatgg ttcgactggg
     3661 atttcggctg gttatgccac agacattcct ccccataatt tagctgaggt catagatgct
     3721 gcagtttaca tgattgacca cccaactgca aagattgata aactcatgga attcttgcct
     3781 ggaccagact tccctacagg ggctattatt cagggtcgtg atgaaatcaa gaaagcttat
     3841 gagactggga aagggcgcgt ggttgttcgt tccaagactg aaattgaaaa gctaaaaggt
     3901 ggtaaggaac aaatcgttat tactgagatt ccttatgaaa tcaataaggc caatctagtc
     3961 aagaaaatcg atgatgttcg tgttaataac aaggtagctg ggattgctga ggttcgtgat
     4021 gagtctgacc gtgatggtct tcgtatcgct atcgaactta agaaagacgc taatactgag
     4081 cttgttctca actacttatt taagtacacc gacctacaaa tcaactacaa ctttaatatg
     4141 gtggcgattg acaatttcac acctcgtcag gttgggattg ttccaatcct gtctagctac
     4201 atcgctcacc gtcgagaagt gattttggcg cgttcacgct ttgacaaaga aaaggctgag
     4261 aaacgtctcc atatcgtcga aggtttgatt cgtgtgattt cgattttgga tgaagtcatt
     4321 gctcttatcc gtgcttctga gaataaggcg gacgccaagg aaaacctcaa agttagctat
     4381 gattttacgg aagaacaggc tgaggctatc gtaactttgc aactgtaccg tttgaccaat
     4441 accgatgtgg ttgtcttgca ggaagaagaa gcagagcttc gtgagaagat tgctatgctg
     4501 gtggctatta tcggtgatga aaggactatg tacaatctca tgaagaaaga acttcgtgag
     4561 gtcaagaaga aatttgcaac tcctcgtttg agttctttag aagacactgc gaaagcaatt
     4621 gagattgata cagctagtct tatcgctgag gaagatacct acgtcagcgt gaccaaggca
     4681 ggttacatca agcgtaccag tccacgttcc tttgcggctt ccaccttgga agaaattggc
     4741 aagcgtgatg atgaccgttt gatttttgtt caatctgcca agacaaccca gcacctcttg
     4801 atgttcacaa gtcttggaaa tgtcatctac agaccaatcc atgagttggc agatattcgt
     4861 tggaaggaca tcggagagca tctgagccaa accatcacaa actttgaaac gaatgaagca
     4921 atcctttatg tggaagtact ggatcagttt gacgatgcga caacctactt tgcagcgact
     4981 cgccttggtc aaatcaaacg ggtagagcga aaagaattca ctccatggcg gacctataga
     5041 tctaagtctg tcaagtatgc taagctcaaa gacgatacag atcagattgt agcagtggct
     5101 ccgattaaac tagatgatgt tgtcttggtt agtcaaaatg gttatgccct gcgtttcaat
     5161 atcgaagagg ttccggttgt cggtgctaag gcagcaggtg tcaaggctat gaatttgaaa
     5221 gaagatgatg tcctccaatc tggctttatc tgtaatactt cgtccttcta cctcttgacc
     5281 cagcgtggaa gcttgaaacg tgtttccatt gaggaaattc tagcaaccag ccgtgccaaa
     5341 cgaggattac aagtcttgcg tgagttgaaa aacaaaccgc atcgtgtctt cttggcagga
     5401 gcagttgcag agcaaggatt tgttggcgat ttcttcagta cggaagtgga tgtgaacgac
     5461 caaactctgc ttgtccaatc caataaagga acaatctatg aaagccgatt gcaagacttg
     5521 aacttgtcag aacgcactag caatggaagc ttcatttctg acacgatttc agatgaagaa
     5581 gtttttgacg cttatcttca ggaagtagtt actgaagata aataagtgaa atgaagaatc
     5641 agttctaaga tggtttttat tggagaaatt ttctgaaaat tacaaaatat acttgctatt
     5701 ttagaaaaat agtgtagaat ataagaaata ggtttttaga ataaggagtg gaatatgaca
     5761 gtaacgattg attgggaaaa cctcggtttt tcctatatga aattacctta tcgctatctt
     5821 gctcatttca aaaatggaca atgggatcaa ggagagctta cagaggatgc aactttgcat
     5881 atttcagagt cttctccaag tcttcactat ggacaacaag catttgaagg tttgaaagct
     5941 tatcgtacta aggatggcag tgttcaactg ttccgtcctg atgaaaatgc taaacgcctg
     6001 caacgtacat gtgaccgtct cttgatgcca caagttccga cagacatgtt tgtagaagct
     6061 tgtaaagcag ttgtccgtgc gaatgaagaa tacgtaccac catacggaat aggtggaact
     6121 ttatatcttc gccctctttt gattggtgtc ggagatatta tcggggtaaa accggcagaa
     6181 gagtacattt tcaccatctt tgctatgcca gttggaaatt actttaaagg tggtttggtc
     6241 ccaaccaact tcttgattca ggatgagtac gaccgtgcag caccaaatgg tacaggtgcg
     6301 gctaaggttg gtggaaacta tgctgcaagt ctcttaccag gaaaaatggc caagtcacgc
     6361 catttctcag atgttatcta tctggaccca tcaactcata caaagattga agaagtcgga
     6421 tcagctaatt tctttggaat tacagctgat aatgaatttg taacaccatt gagtccatct
     6481 atcttgccat ctattaccaa gtattccttg ctttatttgg cagaacatcg cttgggatta
     6541 actcctattg agggtgatgt tccaattgat aatcttgacc gttttgtaga ggcaggtgcc
     6601 tgtggtacag cagcggttat ttctccaatt ggaggtattc aacatggtga tgatttccat
     6661 gtattctata gtgaaacaga agtaggtcct gtgacgcgta aattatataa tgaattgacg
     6721 ggtattcagt ttggcgatat tgaagcgcca gaaggttgga ttgtaaaagt agattaaaat
     6781 aaaccaaagg agatttttta tgaaatagaa aaagtggctc ttaacagcag gagtggtcct
     6841 gagcacgtca gctattttag tggcttgtgg aaaaactgat aaagaagcag atgcaccgac
     6901 aacattttct tatgtctatg cagtagatcc agcatcattg ggctacagta tagcgactcg
     6961 aacatcgagg acagacgtta ttggaaatgt tattgatggt ttgatggaaa atgataaata
     7021 cggcaatgtt gctccttctc aaaaagacta tgatttgaac agtacaggat gggctccaag
     7081 ctatcaagat ccagcgtctt acttgaatat tatggatcca aaatctggtt ctgccatgaa
     7141 acaccttggc attacgaaag gaaaagataa ggatgttgta gctaaacctg gtttggataa
     7201 atataagaaa ttgttagaag atgctgtttc tgagaccact gacctagaga agagatatga
     7261 aaaatatgcc aaagctcaag cttggtcgac agatagttca ttattgatgc caacagcttc
     7321 atctggtggt tctccagttg taagtaacgt agtaccattc tcaaaaccat actcacaagt
     7381 tggtattaag ggggaaccat atatctttaa aggaatgaaa ttgcaaaaag atattgttac
     7441 aacaaaagaa tataacgagg tttttaaaaa atggcaaaaa gaaaaattgg aatccaatag
     7501 caaataccaa aaagaactag aaaaatacat taaataagga atggtattga tcttgataaa
     7561 atttttaaaa tactgtcatt ttgaatataa aggagtttga tatggagtgg attagattaa
     7621 taggaatagc aatcattgtt gtgggtttta ttttaaaatt tgatacaatt gcaacagtag
     7681 tcttagctgg tttggttaca gctttagttt caggtgtttc tctcgttgaa tttttggaga
     7741 ttttgggaaa agaatttagc aatcagcgag tgctcacgat ttttatggtt accttgcctc
     7801 ttgtggggct gtcagaaacc tttggactca agcaacgatc aatcgatttg attcgaaaga
     7861 ttaaaggtct gacagttgga aacttctata cagtttattt ctttattcga gagttagctg
     7921 gtttcttttc aattcgtcta ggaggacacc ctcagtttgt cagacctttg gttcaaccta
     7981 tgggagaagc agctgcagag tctcaattag gtagaaagtt aacagaggtt gaagatgaga
     8041 caataaaagc gcgtgcggct gcgaatgaaa attttggaaa tttctttgct caaaatacgt
     8101 ttgtaggtgc tgggggagtc ctcttgatag ggggaacatt agatcagtta ggctatgaaa
     8161 gcaattatgc agggattgct tctacatcta ttattgttgc tgttataaca cttattgtag
     8221 tggggattta caattattta tttgataaaa aattgatatc aaaaaagact aggggaggag
     8281 aacaaaaatg acagagttgg caaagcaact attagagttg acctatattg tgattggttg
     8341 tcaatttctc catacagcct attgtagtta taaagataaa acaaacccag ttcgacttgg
     8401 gacatctgca ttttggactc tattgtctat tacgtttata ggtggttcct atatgccaaa
     8461 tatgagtatt ggtattattg taatcctatt atcgctgtta acattgttta agcaagtccg
     8521 tatcggaacc ttgccatcct tagatgaaat gaaagccaat attgaatcta acaggttgaa
     8581 aaataaaatt tttattccag ttatgctgat ggcaatactt gcgttggtct tagcgcaaat
     8641 gattccagaa tttagcaaga tttcgattag ccttgccgcc ttgtttgcta caatttctgt
     8701 tcttgtgatt accaatagtc accctaagag tctgttatca gaaaataatc gaatgactca
     8761 gcaagtttca acaagtggga ttgttcctca attattaggg gctttggggg ctatttttac
     8821 tgtagcaggt gttggtgatg ttatctctca tctgattagc ggtattgttc cttcagatag
     8881 tcgctttata ggagttttgg cttatgttct tggaatggtt ctattcacaa tgattatggg
     8941 aaatgctttt gcagcattca ccgttattac agcaggtgtt ggagttccct ttgtatttgc
     9001 tctgggagct aatccaattg tggctggtgc tcttgccatg acagcaggtt attgtgggac
     9061 cttattgacc ccaatggctg ctaattttaa cgctctacca gcagcattga tggatatgaa
     9121 agatcagaat ggcgttataa aggctcaagc aggtgttgct ctagtaatga ttgttattca
     9181 catattctta atgtactttc tcgcatttta gtaaaggaaa ataaaatgaa aatattggtt
     9241 acaggtttta atccttttgg aggtgaaaag attaatccag ctttggaggc tgtaaaatta
     9301 ttaccatctg agattaatgg ggctgaagtt cgctgggtag aaattccaac ggttttttat
     9361 aagtcgtcag aagttttaga ggcagaaata ttacgatatc aaccagatgc tgtactttgt
     9421 attggacaag caggcggcag gaccggctta acacctgaac gagtggctat taatcaagat
     9481 gatgctcgca tacctgataa cgaaggcaat caaccaattg atacaccgat tcgtattgat
     9541 ggagcatcgg cctattttag tagtttacct atcaaagcga tggtacaagc tatcaaaaaa
     9601 cagggacttc cggcagttgt atccaatagt gcaggaacct ttgtttgcaa tcatttgatg
     9661 taccaagctc tttatttagt ggataaaaaa ttccctaata tgagagcagg tttcatgcat
     9721 attccatata tgatggaaca agtagtaaat aagccgaata ctgcaggaat gagtctatgt
     9781 gatattgtaa gaggcataga ggtagctatt gaagctattg tagactacaa agataaggat
     9841 ttgcagttag taggcggtga gactcactga taacctaaaa aggatagtca attatgcttg
     9901 tttactaact attaactatg ctaaatcaat tgaggttgtt tacataaaac tctatatcag
     9961 agaagcctga tatagagttt tttcttgcta gttttaggat tttcttgtaa aatagaaaaa
    10021 gtgaagagag gtatgaaatg agcaagaaag ataaaaaaat cgaaattcaa gtagcggatg
    10081 ccaaagttaa tgttggtaaa gacagttttg aaggttatac attgactatc ggtaaaaaag
    10141 ttatcggaga aattgccgaa ttagacggac aatttgccat tataaagaat gggaatgtcg
    10201 atagttttta taaaaaattg gaaaaagctg tggaaatttt gattgaaaat tataatttag
    10261 caaaataagt cttgtttttt tgaaattttc atgatataat agtccatgtt gattgtagga
    10321 gagatagcga agaggctaaa cgcggcggac tgtaaatccg ctccttcggg ttcgggggtt
    10381 cgaatccctc tctctccatt tcattaatgg ggtatagcca agcggtaagg caagggactt
    10441 tgactccctc atgcgttggt tcgaatccag ctaccccagt tcttaggtaa taatcaagat
    10501 agaaagcaaa atatcttagg gtattttatt tttataattg aaagacgtga atgatatgaa
    10561 catgtccttg cgggtgctta ggaaaaaaat tataagtatg tcaagtttaa gaaaaacttg
    10621 attgttggag gattttttag atgaacgaat ttgaagattt gctaaatagc gttagtcaag
    10681 ttgagactgg tgatgttgtt agtgctgaag tattgacagt tgatgcgact caagctaacg
    10741 ttgcaatctc tggaactggt gttgaaggtg tcttgactct tcgcgaattg acaaacgatc
    10801 gtgatgcaga tatcaatgac tttgttaaag taggagaagt attggatgtt cttgtacttc
    10861 gtcaagtagt tggtaaagat actgatacag ttacatacct tgtatctaaa aaacgccttg
    10921 aagctcgcaa agcatgggac aaacttgttg gtcgcgaaga agaagttgtt actgttaaag
    10981 gaacgcgtgc cgttaaaggt ggactttcag tagaatttga aggtgttcgt ggatttatcc
    11041 cagcttcaat gttggatact cgtttcgtac gtaacgctga gcgttttgta ggtcaagaat
    11101 ttgatactaa aatcaaagaa gttaacgcta aagaaaaccg cttcatcctt tcacgtcgtg
    11161 aagttgttga agcagctact gcagcagctc gcgctgaagt attcggtaaa ttggctgttg
    11221 gtgatgttgt aactggtaaa gttgctcgta tcacaagctt cggcgctttc atcgaccttg
    11281 gtggtgttga cggattggtt cacttgactg aattgtcaca tgaacgtaac gtatcaccaa
    11341 aatcagttgt aactgttggt gaagaaattg aagtgaaaat ccttgatctt aacgaagaag
    11401 aaggacgtgt atcactttca cttaaagcaa cagtaccagg accatgggat ggcgttgagc
    11461 aaaaattggc taaaggtgat gtagtagaag gaacagttaa acgtttgact gacttcggtg
    11521 catttgttga agtattgcca ggtatcgatg gacttgttca cgtatcacaa atttcacaca
    11581 aacggattga aaatccaaaa gaagctctta aagttggtca agaagttcaa gttaaagttc
    11641 ttgaagttaa cgcagatgca gaacgcgtgt cactttctat taaagctctt gaagaacgtc
    11701 cagtccaaga agaaggacaa aaagaagaaa aacgtgctgc tcgtccacgt cgtccaagac
    11761 gtcaagaaaa gcgtgatttc gaacttccag aaacacaaac aggattttca atggctgatt
    11821 tgtttggtga tatcgaactt taatcaaatt gaaaattcac aaaatccttt gtttactaaa
    11881 caagggattt ttcttttgtc tctgctcctt ttttgatata atagttctat gttaaaatca
    11941 gaaaaacaat cacgttatca aatgttaaat gaagaattgt ccttcctatt ggaaggcgaa
    12001 accaatgttt tggctaatct ttccaacgcc agtgctctca taaaatcacg ttttcctaat
    12061 accgtatttg caggctttta tttgttcgat ggaaaggaat tggttttagg tcccttccaa
    12121 ggaggtgttt cctgcatccg tattgcacta ggcaagggtg tttgtggtga ggcagctcac
    12181 tttcaggaaa ctgttattgt tggagatgtg acgacctatc tcaactatat ttcttgtgat
    12241 agtctagcta aaagtgaaat tgtggtgccg atgatgaaga atggtcagtt acttggagtt
    12301 ctggatctgg attcttcaga gattgaggat tacgatgcta tggatcgaga ttatttggaa
    12361 caatttgtcg ctattttgct tgaaaagaca acatgggact ttacgatgtt tgaggaaaaa
    12421 tcttaatgta tcaagcactt tatcgaaaat atagaagtca aaacttctcc cagttagttg
    12481 gtcaagaagt tgtggctaag actcttaaac aagcggtgga gcaagagaaa ataagtcacg
    12541 cttatctttt ttctggtcct cgtggaacgg gaaaaaccag tgttgctaaa atctttgcca
    12601 aggctatgaa ctgtcccaat caagtgggtg gcgaaccttg caataactgc tatatttgtc
    12661 aagcagtgac ggacggtagt ttagaagatg tcattgaaat ggatgcagct tctaataatg
    12721 gggtagatga aattcgcgaa attcgtgata aatctaccta tgcgcctagc cttgctcgtt
    12781 ataaggttta tatcatagat gaggttcaca tgctgtctac aggggctttt aatgccctcc
    12841 taaagacgct ggaagaacca acacagaatg tagtctttat tttggccact actgaattgc
    12901 acaagattcc tgctactatt ctatcccgtg tgcaacgttt tgagtttaaa tcaattaaga
    12961 cacaggatat tcaggaacat attcactata tcttagaaaa agaaaatatc agttctgaac
    13021 cagaggctgt ggaaatcatt gccagacggg cggaaggtgg aatgcgggac gccttgtcta
    13081 ttttggatca agccctgagt ttgacacagg gaaatgagct gacgactgct atctctgaag
    13141 aaattactgg caccattagc ctatcagcct tggatgatta tgtggcggcc ttgtctcaac
    13201 aggatgttcc caaagctttg tcttgcttga atcttctttt tgacaatggt aagagcatga
    13261 ctcgttttgt gaccgatctt ttgcactatt taagagactt gttaattgtt caaacagggg
    13321 gagaaaatac tcatcatagt tcagtctttg tagaaaattt ggcacttcct caaaaaaata
    13381 tgtttgaaat gattcgctta gcaacagtga gtttagcaga tattaagtct agtttgcagc
    13441 ccaagattta tgctgaaatg atgaccgtcc gtttggcgga aatcaagccc gaaccagctc
    13501 tatcaggagc ggttgaaaat gaaattgcta cgctgagaca ggaagttgcc cgtctcaaac
    13561 aagagctttc taatgcaggt gcggttccta aacaagttgc accagctcct agtcgaccag
    13621 ctacgggcaa aacagtctat cgtgtcgatc gcaataaagt gcaatctatc ttacaagagg
    13681 ccgtcgaaaa tcctgattta gcacgtcaaa atctaattcg tttgcagaat gcctggggag
    13741 aggtaattga aagtctaggt gggccggaca aggctctgct agttggttct caaccggttg
    13801 ctgccaatga acaccatgct attcttgctt ttgagtctaa cttcaatgct ggtcaaacta
    13861 tgaaacgaga caatctcaat accatgtttg gtaatatcct cagtcaggcg gcaggttttt
    13921 cacctgagat tttagctatt tccatggagg aatggaaaga agttcgcgca gccttttcag
    13981 ccaaagccaa atcttctcaa actgaaaaag aagtagaaga aagcctgatt ccagaaggat
    14041 ttgaattttt ggctgataaa gtgaaggtag aggaagacta aagaaagatt tcatgataca
    14101 ataagtttat gaataaacaa caatttatta ttatggcgct atttacagct gctgagacct
    14161 attttttcaa tgaagcctgg atgactggcc gctatattat ggcagccttt tgggcaattt
    14221 tactctttag aaatttccga gtcagttatg tgatgggtaa aatcgttgat gtcatcgatc
    14281 agcattttaa taggaaagac tagccctcag cttccagaca aaatcaaagc cttttaggct
    14341 tttttttgtt atactagaaa agtatattta tagaattttt gctctatttc tggggaaatc
    14401 agacgttttt ctagtaagta ctgtaaaagt tttgaaaaag aaaggaacta tcatgtcagt
    14461 aatagagatc aaagatcttc acgttgagat tgaaggaaaa gaaattttaa aaggggttaa
    14521 cctgaccctg aaaacaggag aaattgccgc tatcatggga ccaaatggta caggtaaatc
    14581 gactctttct gccgctatca tgggaaatcc aaactatgaa gtaactaaag gtgaagtttt
    14641 gtttgatggc gtaaacatcc ttgagttgaa agtggatgag cgtgcgcgta tgggactttt
    14701 ccttgctatg caatacccat cagaaatccc tggaattacc aatgctgagt ttcttcgtgc
    14761 cgctatgaat gcgggtaaag aagatgatga gaagatttca gttcgtgagt ttattactaa
    14821 gctagatgaa aaaatggaat tgctcaacat gaaagaagaa atggcagagc gttacctcaa
    14881 cgaaggcttc tctggtggtg agaaaaaacg caatgaaatt cttcaacttt tgatgttgga
    14941 gccaacattt gctcttttgg acgagattga ctcaggtctt gatattgacg ctcttaaagt
    15001 tgtgtctaaa ggtgtcaatg ccatgcgtgg tgaaggtttt ggtgctatga tcatcactca
    15061 ctaccaacgt cttttgaact atatcacacc tgatgtggta cacgtgatga tggaaggtcg
    15121 tgttgtcctt tctggtggtc cagaattggc tgcgcgtttg gaacgtgaag gatacgcaaa
    15181 attagctgaa gaacttggct acgactacaa ggaagaattg taattccctc gtatctttta
    15241 ggagaagtaa atgactagag aaaatattaa acttttttca gaaatgcacg ctgaaccaag
    15301 ctggttggct gatctccgtc aaaaagcttt tgacaagatt gagactttgg aattaccagt
    15361 tattgagcgt gtcaaattcc accgttggaa tctgggtgat ggaacgatta cagaaaatga
    15421 gccatcagca aatgttccag atttcacagc tttagatcat cacttgaagt tggtgcaagt
    15481 aggaactcaa actgttttcg aacaaactcc agttgagtta gctgaacagg gtgttgtctt
    15541 cacagacttt cactcagctt tagaagaaat tccagagctg atcgaagaat tcttcatgtc
    15601 atctgttaag tatgatgatg acaagttggc ggcttaccac acagcttact ttaacagtgg
    15661 tgctgtactc tatattccag ataacgtaga aattacagag ccaattgaag gaattttcta
    15721 ccaagatagc gatagcaatg tgccgtttaa caagcatatt atgattatcg ctggtaaaaa
    15781 ttctaagatt agttatctgg agcgtttaga gtcacgcggt gaaggaagtg acaaagcaac
    15841 tgccaatatc acagtggaag tgattgcacg ttctggtgcg caagtcaagt ttgctgctat
    15901 cgaccgtcta ggtgaaaacg tcactgccta cattagccgt cgtggtaaat taggcaacga
    15961 tgcaagtatt gactgggcta tcggtgtcat gaacgaagga aatgtcgttg ctgattttga
    16021 tagtgacttg attggtaatg gtagccatgc tgacctgaag gttgtagctc tttcaagtgg
    16081 tcgtcaggta caagggattg atactcgtgt aactaactat ggctgcaact ctatcggaaa
    16141 tatcctacaa catggggtta tccttgaaaa agcaactttg actttcaatg gtatcggcca
    16201 catcatcaag ggtgctaagg gagcagatgc ccaacaagag agccgtgttc tcatgctttc
    16261 agaccaagcg cgttcagatg ctaacccaat tcttttgatt gatgaaaatg atgtcactgc
    16321 aggacacgca gcgtctatcg gacaagttga tccagaagac atgtattacc tcatgagtcg
    16381 tggattggat aaggcaactg cagagcgttt ggttgttcgt ggtttccttg gatctgttat
    16441 cgtggagatt ccagtcaagg aagttcgtga tgaaatgatt gcaactatcg aagagaaatt
    16501 gtcaaaacgc taaggggaag cctatgttag atgtagaagc gattcgcaag gattttccaa
    16561 ttttagatca gattgtcaat gatgaacctc tggtctatct ggacaatgct gcgacgacac
    16621 aaaaaccact agtagttctg aaagttatta acagctacta tgagcaggac aatgccaatg
    16681 ttcaccgtgg tgtccatacc ttagcggaac gagcgacagc ttcttatgaa gctgctcgtg
    16741 aaaccattcg taagtttatt aatgcaggct ctacaaagga agttcttttt accagaggaa
    16801 cgacaaccag ccttaactgg gtagcacgct ttgctgagga aattctcact gagggagacc
    16861 aggtcttgat ttcagtaatg gaacaccatt ctaatatcat tccatggcag gaagcttgtc
    16921 gaaagactgg agcagagctt gtctatgtct atcttaaaga cggtgccttg gatatggagg
    16981 atttgcgagc taaattgact gataaggtta aatttgtttc cctagctcat gtctccaatg
    17041 ttcttagtgt ggtcaatccg atcaaggaaa tcactcaatt agcccaccaa gttggggcaa
    17101 tcatggtagt ggatggtgct caatctacac ctcatatgaa gattgatgtc caggacttgg
    17161 atctggactt tttcgccttt tcgggtcaca agatggctgg tccgactggt atcggtgtcc
    17221 tttacggcaa agaaaagtat cttgagcaaa tgtctccagt agaatttggc ggcgagatga
    17281 ttgattttgt ctacgagcaa tccgctagtt ggaaggaatt gccttggaaa tttgaggctg
    17341 gaacgccaaa tatggcagga gctattggac ttgcgactgc agttgattat ctggaaaaga
    17401 ttggtatgga tgccgttgaa gctcatgaac aggaattgat tgcgtacgtc tatccaaaac
    17461 tgcaggcaat tgagggattg accatttacg gttctcagga tttggctcaa cgttcgggtg
    17521 ttattgcctt taacctaggt gatctccatc ctcacgatct tgcgacggct ctggattatg
    17581 aaggagtggc tgttcgtgct ggtcaccatt gtgcgcaacc cttgcttcag tatttggaag
    17641 tcccagcaac agctcgtgca agtttttata tctacaatac caaggcagat tgcgacaaac
    17701 tagtcgatgc cctacaaaag acaaaggagt ttttcaatgg cactttctaa actagatagc
    17761 ctttatatgg cagtggtagc agaccattcg aaaaatccac atcaccaagg gaagttagaa
    17821 gacgctgagc aaatcagtct caacaatccg acttgtgggg atgtcatcaa cctctctgtc
    17881 aagtttgatg cagaggaccg tttggaagat attgcttttc taaattcagg atgcacgatt
    17941 tcaactgctt ctgctagtat gatgacagat gccgttttag gaaaaaccaa acaagaaatt
    18001 ttagaactgg cgactatttt ttctgaaatg gttcaagggc aaaaagatga gcgtcaagac
    18061 caacttggag acgcggcatt cttgtcaggt gttgccaaat tccctcaaag aatcaagtgt
    18121 gcaaccctag cttggaatgc ccttaagaaa acaattgaaa atcaagaaaa acagtaagac
    18181 aagtttcttt tgtcttatga attattagaa atgaagaaag aaaggatact atggctgaag
    18241 aaagagtaga accaaaacca attgaccttg gtgaatataa atttggtttc catgacgatg
    18301 tagagcctgt cttatcgaca ggaaaaggac tcaacgaagg tgttattcgt gaattatctg
    18361 ctgctaaggg tgagcctgag tggatgttgg agttccgttt gaagtcttat gaaaccttca
    18421 aaaaaatgcc catgcaaact tggggagcag acttgtcaga gattgacttt gatgacttaa
    18481 tctactacca aaaaccatct gacaaaccag cccgttcttg ggatgatgta cctgaaaaga
    18541 ttaaagaaac ctttgaacgt atcgggattc cagaagctga acgtgcttat ttagcagggg
    18601 cttctgccca gtacgagtca gaagtggttt accacaacat gaaggaagag ttccaaaaat
    18661 taggtattat ctttacagat acagattccg cactcaagga atacccagac ttatttaaac
    18721 aatactttgc gaagttggta ccgccgacag ataacaagtt ggcagccctc aactcagcag
    18781 tatggtcggg tggaactttt atctacgtgc caaaaggtgt caaggtagat attccacttc
    18841 aaacttattt ccgtatcaat aacgaaaata taggtcagtt cgaacgtacc ttgattatcg
    18901 ttgatgaggg agcaagcgtc cactacgtag aaggatgtac agcaccaaca tattcaagca
    18961 atagcttaca cgctgccatt gtagaaattt ttgctttgga cggagcttat atgcgttata
    19021 caactatcca aaactggtct gataacgtct ataacttggt aacaaagcgt gctaaggctc
    19081 aaaaggatgc cactgttgag tggattgatg gaaacttggg tgccaaaacg actatgaaat
    19141 atccatctgt ttaccttgat ggagaaggag cgcgtggtac catgctctct atcgcctttg
    19201 ctaatgcagg gcaacaccaa gacacgggtg ctaagatgat tcacaatgct ccacatacca
    19261 gctcgtctat tgtgtctaaa tccatcgcta aaggtggagg aaaggttgac taccgtggac
    19321 aagtcacctt taacaagaac tctaagaaat ctgtttccca cattgaatgt gataccatta
    19381 tcatggatga cttgtcagca tcagatacta ttccatttaa tgaaattcac aactcgcaag
    19441 tggctttgga acacgaagcc aaagtatcta agatttcaga agagcaattg tattatctca
    19501 tgagccgtgg attgtcagaa tctgaggcaa ctgaaatgat tgtcatggga tttgtagaac
    19561 cctttacaaa agaacttcca atggaatacg cagttgagct gaaccgcttg attagctatg
    19621 aaatggaggg atcagttgga taaaatttga ttttatactc ttcgaaaatc tcttcaaacc
    19681 acgtcagcat cgccttaccg tatgtatggt tactgacttc gtcagtttca tctacaacct
    19741 caaaacagtg ttttgagcaa cctgcggcta gcttcctagt ttgttctttg attttgaaga
    19801 tgaacttgta tcaaaaaatc gcggtttaaa atcgcgattt tttataattt ctcgttaaca
    19861 aagcggacaa actgattcca ccaaactttt aagaagaagg ctttttcaat tttcttgtct
    19921 gctaccattt cgaaactagg gcgctctgtg gtgatgtaac cttgaccaat caagtccttg
    19981 tcttcataag tcaaatggcc aaccactgtt ccagcttcaa gtggtgctgg gattgctttg
    20041 gaatcaggtg tgaattgaac agattgggaa gattgattcc caacacgttc gattagatag
    20101 atatcctctg gagccactgc agttactgta tcttcttttc catcttgtac aggggctttg
    20161 ctatcttgat aggcatcgcc ttgttgaacg attttgcgaa gtgtaaatgt agaagaaata
    20221 taatccatta gggaagatgt agctgtaaat cgagcgtaag gattattgtc ttgatgatct
    20281 gcatttaaaa caactgtgat gactctcatg cctttttcga cagtagtacc aacaaaagac
    20341 tctccagcct tatctgttgt tcctgttttt agcccatcaa aaccaccacg gtaagcaggc
    20401 ataccttcta acatgtagtt ggttgaagtg attgtcatcc cagcaaaagt agaagaaggt
    20461 tttttggtga tttctaagac ttgtgggtat tttttgatga gattgcgagc aacgatagcg
    20521 acatcataag cactaagctt attttcctca tcttttttag aacctgggta aatgttatcc
    20581 cctagagttt cattgttaag acctgtcgta ttgacaacag tggcatcctg aattccccat
    20641 tccaagagtt ttgcccgcat catatcgacg aaatcttttt ctgagccagc aattttctca
    20701 gctagggcaa tagcggcgct gttggcacta gataccagag ttgcttcaag caactcttcg
    20761 acagtataat tacgggcctc cataggaata ttactggctt cagaatttgt cgtcaattga
    20821 taaggataat cagaaatatc tacaggagtg gagagggtaa tacttccgtt ttccaaagct
    20881 tcatagacca gataaacagt aatcaatttt gttatggaag caatttcgac aggttgcgtt
    20941 gcatccttct catagagaat tttaccagta tttgcctcaa cagcaatcgc atgtttagcg
    21001 gcaatggtaa aatcttgagc aacagcagta gaagcacccc ctaaaagaga gacagttaac
    21061 aaagttaaaa atattttttt catagtagtc ttattctatc ataaagaaaa aaaatattct
    21121 tgctttaata attcatctgt taagcttttt gaaaatatgg taaaataaag tgagggaggt
    21181 aactcatgtt tcgtagaaat aaattatttt tttggaccac agaaatttta ctcttaacca
    21241 tcatctttta cctatggaga cagatggggt ctttgattaa cccttttgtt agcgtgctta
    21301 atacaattat gattccattt ttattagggg gctttcttta ttatttgaca aaccctattg
    21361 ttattttctt aaataaagtc tgtaaactca atcgtttgct tggtatttta attaccttgt
    21421 gtactttggt ctggggaatg gtcataggtg ttgtctatct cttacctatt ttgattaatc
    21481 agttatctag tttgattata tctagtcaaa ctatttatag tcgagtacaa gacttaatca
    21541 tagacttatc taattatcct gcgctccaga atttggatgt agaagctaca attcagcagt
    21601 taaacttatc ctatgttgat attcttcaaa atatcctaaa tagcgtatca aatagtgtgg
    21661 ggagcgtctt gtcagctctt atcagtactg ttttgatttt gattatgact ccagtttttt
    21721 tggtttattt cttattagat ggacataaat tcttgcccat gcttgaaaga acgattctaa
    21781 agagggatcg cttgcatatt gcaggcttat taaagaattt aaatgcgacg attgctcgct
    21841 atattagtgg agtttcgatt gacgcaatca ttataggttg tttggcttat attggctata
    21901 gtattattgg tttaaaatat gctttagttt ttgccatttt ttctggtgta gccaatttaa
    21961 ttccttatgt ggggccaagt attggtttga ttcctatgat catcgcaaat atattcactg
    22021 atccccatag actgctgatt gcagtgattt atatgcttgt tgttcagcag gtagatggca
    22081 atatcttata tcctcgaatc gtaggaagtg ttatgaaggt tcatccaatc acgattttag
    22141 ttttactttt gttgtcaagc aatatctatg gtgtagttgg aatgattgtc gcagtgccaa
    22201 cctattctat cttgaaagaa atttctaagt tcttatcccg tttgtatgaa aatcataaaa
    22261 taatgaaaga acgagaaaga gaattagcta agtaaaagtc aggagaaccc tgatttttct
    22321 ttactggaag tggcctttag attagaagac tgaaaataag ttaaagtctt aaactaattt
    22381 tcacagctaa gaatagtaga agttaatctg ataaaaatcg aaaaaaccag tggaattctg
    22441 tgtcagggta agttccactg gttttcatag tctattaaag ttcgaatgaa acctatttat
    22501 agtagattga aactagaata gtacacctct aattctaaaa cattgttaga aatcgatttg
    22561 actgtcctga tctattcgtt ctattcttat ttcattttac tatattttgg tgcaataagt
    22621 gaaaagtagt ccgaataata taaggattga ttttatagtt tttaaactca aatgaattga
    22681 aataaagaga gtacgaaaat tctcatctga aagtatttta gaataattct cttcgtgaat
    22741 ttcttcaaaa cagatagctt catcttaggt atgtgatttc tttttgcatt tttgagttag
    22801 ataaggtata atggttttat tgtcttttgg ggtcgttacg gattcgacag gcattatgag
    22861 gcatattttg cgactcgtgt ggcgacgtaa acgctcagtt aaatataact gcaaaaaata
    22921 acacttctta cgctctagct gcctaaaaac cagcaggcgt gacccgattt ggattgctcg
    22981 tgttcaatga caggtcttat tattagcgag atacgattaa gccttgtcta gcggtttgat
    23041 aagagattga tagactcgca gtttctagac ttgagttatg tgtcgagggg ctgttaaaat
    23101 aatacataac ctatggttgt agacaaatat gttggcaggt gtttggacgt gggttcgact
    23161 cccaccggct ccattattcc tttgcattct tttgcattcc ttggtaaaac gttgttaaat
    23221 caacgttttt tatttttatc tttggtattc ctttgcattc ttttgctaaa aagggagtca
    23281 caaacagacc ctattttaaa aaaaggatag aaaaaaggat acaacatttg tcgcatccta
    23341 aaaataatct tttttcgacg gaagacatgg gattcgaacc cacgcacgct attacacgcc
    23401 taccgcgttt ccaacacggc ctcttaagcc tcttgagtaa tcttccaata cttactcaaa
    23461 tagtctacca taaaggctct tatcttgcaa taaaaattct agaaataaga aaaaatgata
    23521 gattttgaaa gaaaatgata aaaaatgctt gacttcgaaa gaaagtatga tagaatgaat
    23581 agtgtaaacg ataacaggag gtgattcagt gttaaaaaca gaacgtaaac aactaatttt
    23641 agaggagtta aatcagcatc atgtagtttc tctagaaaaa ttagttagtt tgctagaaac
    23701 gtcagaatca acggttcgaa gagacttgga tgagttggaa gcggaaaaca agcttcgtcg
    23761 tgtgcatggt ggagcagaac tcccccactc cttacaggaa gaagaaacca ttcaagaaaa
    23821 atctgtcaaa aaccttcaag aaaagaaatt gctggctcag aaagcagcct ctctcattaa
    23881 agaaaaagat gtcatcttta tcgatgctgg aacaacaact gcttttttga ttcatgaatt
    23941 ggtcaataag aatgttacag ttgtgaccaa ctccattcac catgccgctc agttggttga
    24001 aaagcagatt ccaactgtca tggttggagg aaacgtcaag acggcgacag atgctagtat
    24061 cgggggcgtt gctcttaacc agattaacca attgcacttt gaccgtgcct ttatcggaat
    24121 gaatggtgtt gacgatggct attatacgac tcctgatatg gaggagggag ctgtgaaaag
    24181 agctattttg gagaatgcca agcagaccta cgtcttggtg gattcgtcaa aaattggaca
    24241 aacttgcttt gccaaggtag ccccactcaa acgcgctatc gttatcacta gtcaagggca
    24301 tgagctcttg caggttatta aggagaaaac ggaggtaata gaagtatgat ttatacagtc
    24361 acactcaatc catccattga ctatatcgtt cgtttggacc aagtcaaagt tggtagtgta
    24421 aatcgtatgg acagtgatga taagtttgct ggtgggaaag gaatcaatgt cagccgtgtc
    24481 ttgaaacgtt tgaatatatc aaatacagcg acgggattta tcggtggctt tactggtaaa
    24541 tttatcacag atactttagc agaggaagaa atcgagacac gttttgtcca ggtggcagaa
    24601 gatactcgta tcaatgttaa aatcaaagca gaccaagaaa cagaaatcaa cggaacgggt
    24661 ccaactgttg aaccggttaa gctagaagaa ttgaaagcta ttttatctag tctgacagca
    24721 gaagatacag ttgtctttgc aggttcaagt gctaaaaatc taggcaatgt tatctataag
    24781 gatttgattt ccttgacgcg ccagactggt gcgcaagtgg tctgtgactt tgaaggacag
    24841 accttaattg atagtttgga ctaccagcct cttcttgtaa aaccaaataa tcatgaactt
    24901 ggagcgattt ttggggttaa actcgaaagt ttagatgaaa ttgagaaata cgctcgtgag
    24961 ttactggcta agggtgctca aaatgttatt atctctatgg ctggtgatgg tgcccttctt
    25021 gtcacatctg agggagctta cttcgctaaa ccaatcaaag gaacagtcaa aaattcagtt
    25081 ggagctggtg attctatggt tgctggattc acaggtgaat ttgtcaaatc aaaagacgca
    25141 gtagaagcct tcaaatgggg agtggcttgc ggaacggcaa ctaccttctc agatgacttg
    25201 gcaacggcgg aatttattaa agaaacatat ggaaaagttg aggtagaaaa acgatgaaaa
    25261 ttcaagattt attgagaaaa gatgtcatgt tgctggattt gcaggcaact gaaaagactg
    25321 ctgtcattga agagatgatt aaaagcctaa caggccacgg ttatgtgaca gattttgaaa
    25381 cctttaaaga aggcattttg gcgcgtgaag ctttaacttc tactggtttg ggtgatggaa
    25441 tcgctatgcc tcacagcaaa aacgctgctg tcaaagaagc gacagttctc tttgctaagt
    25501 caaacaaggg tgttgactat gagagtttgg atgggcaggc aactgacctc ttcttcatga
    25561 ttgcagctcc agaaggtgcc aatgatactc acttggcagc attgacagaa ttgtctcaat
    25621 acttgatgaa agacggtttt gcagacaaac ttcgtcaagc aacatctgca gaccaagtta
    25681 tcgaactttt tgaccaagct tcagaaaaaa ctgaggaact tgttcaagca cctgctaatg
    25741 actctggtga ctttatcgta gctgttacag cttgtacaac aggtattgcc cacacttaca
    25801 tggcccaaga agcccttcaa aaagtagctg ctgaaatggg ggttggtatc aaggtcgaaa
    25861 ccaacggtgc tagcggtgtt ggaaatcaac taactgcaga agatatccgt aaggctaaag
    25921 ctattatcat tgcagcagac aaggccgttg aaatggatcg tttcgatggc aaacctttga
    25981 tcaatcgtcc agttgctgac ggtatccgta agacagaaga attgatcaac ttggctattt
    26041 caggagatgc ggaagtctac cgtgccgcta atggtgccaa agtcgcaaca gcctctaacg
    26101 aaaaacaaag ccttggtggt gccttctaca aacacttgat gagtggtgta tctcaaatgt
    26161 taccattcgt tatcggtggt ggtatcatga ttgcccttgc cttcttgatt gacggtgctt
    26221 tgagtgttcc aaatgaaaac cttggcaatc ttggttctta ccatgagtta gcttctatgt
    26281 tcatgaaaat tggtggagct gcctttggtt tgatgcttcc agtctttgcg ggttatgttg
    26341 cctactctat tgctgaaaaa ccaggtttgg tagcaggttt cgtggctggt gctattgcca
    26401 aagaaggttt tgcctttggt aaaattcctt atgccgcagg tggtgaagca acttcaactc
    26461 ttgcaggtgt ctcatctggt ttcctaggtg cccttgttgg tggatttatc gcaggtgcct
    26521 tggttcttgc catcaagaaa tacgttaaag ttcctcgttc actcgaaggt gctaaatcaa
    26581 tccttctatt gccacttctt ggaacaatct tgacaggatt tgttatgcta gctgtgaata
    26641 tcccaatggc agcaatcaac actgctatga atgacttcct aggcggtctt ggaggaggtt
    26701 cagctgtcct tcttggtatc gtccttggtg gaatgatggc tgttgacatg ggtggaccag
    26761 ttaataaagc agcttatgtc tttggtacag gtacgcttgc agcaactgtt tcttcaggtg
    26821 gttctgtagc catggcagca gttatggctg gaggaatggt gccaccactt gcaatctttg
    26881 tcgcaactct tcttttcaaa gataaattta ctaaggaaga acgtaactct ggtttgacaa
    26941 acatcatcat gggcttgtca tttatcactg agggagcgat tccatttggt gccgctgacc
    27001 cagctcgtgc gattccaagc ttcatccttg gttcagcagt agcaggtgga ctcgttggtc
    27061 ttactggtat caaactcatg gcgccacacg gaggaatctt cgttatcgcc cttacttcaa
    27121 atgctctcct ttacctcgtt tctgtcttgg taggagcaat cgtaagtggt gtggtttatg
    27181 gttacctacg caaaccacaa gcataaaaaa tagaaaaatg aaaagattgg accgtttggt
    27241 gcagtctttt tctcttcccg aaatgcctgt gaaatatggt ataatagaag aatggcaaac
    27301 aagaatacaa gtacaacaag acggagaccg tctaaagcag aactggaaag aaaagaagcg
    27361 attcaacgaa tgttgatttc gttaggaatt gcgattttat tgattttcgc agccttcaaa
    27421 ttaggggctg caggtataac cctttataat ttaattcgct tgctagtggg tagcctagct
    27481 tatctggcga tattcggcct attaatctat ctcttctttt tcaagtggat acgaaaacag
    27541 gaaggactct tatctggctt tttcaccata tttgctggct tactcttgat ttttgaggcc
    27601 tacttggttt ggaaatatgg tttggacaag tccattctaa aagggaccat ggctcaggtt
    27661 gtgacagatc tgactggttt tcgaacgact agctttgctg gagggggctt gatcggggtc
    27721 gctctttata ttccaacagc ctttctcttt tcaaatatcg gaacttactt tattggttct
    27781 atcttgattt tagtgggttc tctcctagtc agcccttggt ctgtttacga tattgctgaa
    27841 tttttcagta gaggctttgc caaatggtgg gaagggcacg agcgtcgaaa agaggaacgc
    27901 tttgtcaaac aagaagaaaa agctcgccaa aaggctgaga aagaggctag attagaacaa
    27961 gaagagactg aaaaagcctt actcgatttg cctcctgctg atatggaaac gggtgaaatt
    28021 ctgacagagg aagctgttca aaatcttcca cctattccag aagaaaagtg ggtggaacca
    28081 gaaatcatcc tgcctcaagc tgaacttaaa ttccctgaac aggaagatga ctcagatgac
    28141 caagatgttc aggtcgattt ttcagccaaa gaagcccttg aatacaaact tccaagctta
    28201 caactctttg caccagataa accaaaagat cagtctaaag agaagaaaat tgtcagagaa
    28261 aatatcaaaa tcttagaagc aacctttgct agctttggta ttaaggtaac agttgaacgg
    28321 gccgaaattg ggccatcagt gaccaagtat gaagtcaagc cggctgttgg tgtaagggtc
    28381 aaccgcattt ccaatctatc agatgacctc gctctagcct tggctgccaa agatgtccgg
    28441 attgaagcac caatccctgg gaaatcccta atcggaattg aagtgcccaa ctccgatatt
    28501 gccactgtat ctttccgaga actatgggaa caatcgcaaa cgaaagcaga aaatttcttg
    28561 gaaattcctt tagggaaggc tgttaatgga accgcaagag cttttgacct ttctaaaatg
    28621 ccccacttgc tagttgcagg ttcaacgggt tcagggaagt cagtagcagt taacggcatt
    28681 attgctagca ttctcatgaa ggcgagacca gatcaagtta aatttatgat ggtcgatccc
    28741 aagatggttg agttatctgt ttacaatgat attccccacc tcttgattcc agtcgtgacc
    28801 aatccacgca aagccagcaa ggctctgcaa aaggttgtgg atgaaatgga aaaccgttat
    28861 gaactctttg ccaaggtggg agttcggaat attgcaggtt ttaatgccaa ggtagaagag
    28921 ttcaattccc agtctgagta caagcaaatt ccgctaccat tcattgtcgt gattgtggat
    28981 gagttggctg acctcatgat ggtggccagc aaggaagtgg aagatgctat catccgtctt
    29041 gggcagaagg cgcgtgctgc aggtatccac atgattcttg caactcagcg tccatctgtt
    29101 gatgtcatct ctggtttgat taaggccaat gttccatctc gtgtagcatt tgcggtttca
    29161 tcaggaacag actcccgtac gattttggat gaaaatggag cagaaaaact tcttggtcga
    29221 ggagacatgc tctttaaacc gattaatgaa aatcatccag ttcgtctcca aggctccttt
    29281 atctcggatg acgatgttga gcgcattgtg aacttcatca agactcaggc agatgcagac
    29341 tacgatgaga gttttgatcc aggtgaggtt tctgaaaatg aaggagaatt ttcggatgga
    29401 gatgctggtg gtgatccgct ttttgaagaa gctaagtctt tggttatcga aacacagaaa
    29461 gccagtgcat ccatgattca gcgtcgtttg tcagttggat ttaaccgtgc gacccgtctc
    29521 atggaagagc ttgagatggc aggtgtcatt ggtccagctg aaggtaccaa gccaagaaaa
    29581 gtattgcagc aataaaaaat agcttctttc caaggttgga aggagctatt ttactggcta
    29641 ttgattactt ttatactctt cgcaaatctc ttcaaaccac gtcagctttg ccttgccgta
    29701 tatatgtgac tgacttcgtc agtcttatct acaacctcaa aacagtgttt tgagcagcct
    29761 acggctagct tcctagtttg ttctttgatt ttcattgagt attattttct gaagttggag
    29821 tattgggctg tttttcattt tcagtggcag gtttacttgt agcaggtgta gtagagtcct
    29881 gagttgctgt tttctgatct tcttttttct cttccttgac gctagatttt ggcgtttctt
    29941 cttgttgtgt tttttcttga gaagtgttat tttccttatt tggagtagta ttttcctgag
    30001 gggattcttt ttgaatttct ttgacaaagg ttgtcgtctg gcttgtcgta ggttcttttt
    30061 tagtattttt attattatta tccaaggcgt tcatgatcgt gatacccgct gtaatcaaac
    30121 caaggatact accgatagta gtaatggttt tttgaaaaac agtaaagcct tttttgatat
    30181 gttctgtttt cggttttgaa gttctacgat agttagacat acattctctc ctttgattat
    30241 gatttattat actctgtttc tttaaaaaaa tcttaaaaaa ag
//
LOCUS       DAVKQL010000026        29899 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVKQL010000026 DAVKQL010000000
VERSION     DAVKQL010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29899
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.026"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            657..1916
                     /gene="ltrA"
                     /locus_tag="VW561_001063"
     CDS             657..1916
                     /gene="ltrA"
                     /locus_tag="VW561_001063"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEU9943335.1"
                     /translation="MSKLLDKILSRENMLEAYNQVKSNKGSAGIDGMTIEEMDNYLRQ
                     NWRLTKELIKQRKYKPQPVLKVEIPKPDGGIRQLGIPTVMDRMIQQAIVQVMSPICEP
                     HFSDTSYGFRPNRSCEKAIMKLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     EDGDTESLIRKYLHSGVIINGQRYKTLVGTPQGGNLSPLLSNIMLNELDKELEKRGLR
                     FVRYADDCVITVGSEAASKRVMYSVSRFIEKRLGLKVNMTKTKITRPRELKYLGFGFW
                     KSSDGWKSRPHQDSVRRFKFKLKKLTQRKWSIEQLNLSIRGWINYFSLGNMKSIVASI
                     DERLRTRLRVIIWKQWKKKSRRLWGLLKLGVPKWIADKVSGWGDHYQLVAQKSVLKRA
                     ISKPVLEKRGLVSCLDYYLERHALKVS"
     gene            2372..3550
                     /locus_tag="VW561_001064"
     CDS             2372..3550
                     /locus_tag="VW561_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9943336.1"
                     /translation="MSNLFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFTAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTFIILVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEISSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            3864..3995
                     /locus_tag="VW561_001065"
     CDS             3864..3995
                     /locus_tag="VW561_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEU9943337.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            4016..5566
                     /gene="dltA"
                     /locus_tag="VW561_001066"
     CDS             4016..5566
                     /gene="dltA"
                     /locus_tag="VW561_001066"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEU9943338.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMKNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            5563..6807
                     /gene="dltB"
                     /locus_tag="VW561_001067"
     CDS             5563..6807
                     /gene="dltB"
                     /locus_tag="VW561_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEU9943339.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMRGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            6821..7060
                     /gene="dltC"
                     /locus_tag="VW561_001068"
     CDS             6821..7060
                     /gene="dltC"
                     /locus_tag="VW561_001068"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEU9943340.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            7053..8321
                     /gene="dltD"
                     /locus_tag="VW561_001069"
     CDS             7053..8321
                     /gene="dltD"
                     /locus_tag="VW561_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEU9943341.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            8476..8916
                     /gene="adcR"
                     /locus_tag="VW561_001070"
     CDS             8476..8916
                     /gene="adcR"
                     /locus_tag="VW561_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEU9943342.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            8916..9620
                     /locus_tag="VW561_001071"
     CDS             8916..9620
                     /locus_tag="VW561_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU9943343.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYCVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            9613..10419
                     /locus_tag="VW561_001072"
     CDS             9613..10419
                     /locus_tag="VW561_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU9943344.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            10429..11934
                     /gene="adcA"
                     /locus_tag="VW561_001073"
     CDS             10429..11934
                     /gene="adcA"
                     /locus_tag="VW561_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEU9943345.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNIAPVKAEHFHIFFGGTSQETLFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(13105..13878)
                     /locus_tag="VW561_001074"
     CDS             complement(13105..13878)
                     /locus_tag="VW561_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9943346.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTVPDQKLLDAVELFDGEWVSDIQ"
     gene            14054..15457
                     /locus_tag="VW561_001075"
     CDS             14054..15457
                     /locus_tag="VW561_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEU9943347.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLLQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGSTEGTAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            15572..16210
                     /locus_tag="VW561_001076"
     CDS             15572..16210
                     /locus_tag="VW561_001076"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HEU9943348.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMDLMAEKFKTYGQRK"
     gene            16222..16662
                     /locus_tag="VW561_001077"
     CDS             16222..16662
                     /locus_tag="VW561_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HEU9943349.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            16655..17086
                     /locus_tag="VW561_001078"
     CDS             16655..17086
                     /locus_tag="VW561_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9943350.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVNELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISSYDSKINLEEIVQNAQNSLFNVKQQLNVEEEEDLCL"
     gene            17077..17547
                     /locus_tag="VW561_001079"
     CDS             17077..17547
                     /locus_tag="VW561_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9943351.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            17572..18345
                     /locus_tag="VW561_001080"
     CDS             17572..18345
                     /locus_tag="VW561_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9943352.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            18342..19142
                     /locus_tag="VW561_001081"
     CDS             18342..19142
                     /locus_tag="VW561_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9943353.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            19201..21495
                     /locus_tag="VW561_001082"
     CDS             19201..21495
                     /locus_tag="VW561_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEU9943354.1"
                     /translation="MKLWYKKAASNWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKDCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPKTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQEREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            21505..24621
                     /locus_tag="VW561_001083"
     CDS             21505..24621
                     /locus_tag="VW561_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEU9943355.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVPFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKRSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKGDSPSWWEIDLAQTEELEKLIIYNRTDAE
                     IQRLSNFDIIIYDSNNHEVFKQHIDSLESNNLSIDLKGLKGKKVRISLRNAGIPLSLA
                     EVEVYTYK"
     gene            24678..26444
                     /locus_tag="VW561_001084"
     CDS             24678..26444
                     /locus_tag="VW561_001084"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEU9943356.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHTQKGIPAFGIYGRDVQEANDTAIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERALKWVKENVKEGFDHNREDLVLSREEKDRQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPIMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            26590..27741
                     /locus_tag="VW561_001085"
     CDS             26590..27741
                     /locus_tag="VW561_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEU9943357.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKVLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIVEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            27886..28770
                     /locus_tag="VW561_001086"
     CDS             27886..28770
                     /locus_tag="VW561_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU9943358.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(28807..28965)
                     /locus_tag="VW561_001087"
     CDS             complement(28807..28965)
                     /locus_tag="VW561_001087"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943359.1"
                     /translation="MKIKEQTRKLATGCSKHRFKVVDKTDEVSSKHCFEVADRTDEVS
                     NIYTARRR"
     gene            29169..29420
                     /locus_tag="VW561_001088"
     CDS             29169..29420
                     /locus_tag="VW561_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU9943360.1"
                     /translation="MKEFQFERKQRFSLRPYAIGACSVLLGTSLFFDGMGDQPVQDTE
                     TSSALILSHYLDEQDLSEKLKSELQWLELENKLLNLWEH"
     gene            29408..29698
                     /locus_tag="VW561_001089"
                     /pseudo
     CDS             29408..29698
                     /locus_tag="VW561_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         9447 a         4909 c         6386 g         9157 t
ORIGIN      
        1 gtgtaagtcc tcggtgggca cctgctaccg gtgaacccaa tagcgattcc caagcctgac
       61 tatcgtgagg tagcagggag aggaagggat agcgaaatcg tggctctacg aacaggaacg
      121 tgatagtaag gcgtatatag cggataagga ggcttcaaac tctaaagtcc aaaaaggtag
      181 tcgtaaccta tatgtgtaaa tcacgagagt aattgaattc ggactaaggt ttgtgtgaaa
      241 aagataaatc tttctagagt ctaaagactc tgcgtcagat ttcctatttt cactgtaacc
      301 ttttaacgtc ctcatatctt gtataaacga ggaaagatgt acgacttatc ccgtgaggtt
      361 tcatgagcgc tgaaagcgta gtaacaacga atcatgagaa gtcagccgaa cccatagtag
      421 tgaggaaact tctgtagtgg aagtggagcg aaggggtgaa tactcaaaca gtctggggag
      481 agactgtttg aggtctgtcg ctagaaagag aaaacgacag atcgaagtaa tcctacttca
      541 cttgtgtctg taaaatgagt ggtctgatag aactggactt tgacacgtaa tgactagatg
      601 aaggttcacc aatataagat gtccctcagg caccaaaaca agaaaggaat acgcacatgt
      661 caaaactgct agataagata ttatcacgcg aaaatatgct ggaagcctac aatcaagtaa
      721 aatccaataa aggctcagct gggattgatg gaatgactat cgaagagatg gataattatc
      781 tcagacaaaa ctggcgcttg actaaggaac tgataaaaca gagaaaatat aagcctcaac
      841 cagttcttaa agttgagata cctaaaccag acggaggcat ccgtcaacta ggaattccaa
      901 cagttatgga tagaatgatt caacaggcca ttgtccaagt catgagcccc atttgtgaac
      961 cccatttctc agatacgagt tatggtttca gaccaaatag gtcatgtgaa aaagccatca
     1021 tgaagctctt agaatactta aatgacggct atgagtggat agtggatata gacctagaga
     1081 aatttttcga tacagttcct caagatagat tgatgtcctt agtacataac attatcgaag
     1141 acggagatac ggaatccttg attcgtaagt atcttcattc aggtgttatc attaatggtc
     1201 aacgttataa aacactagtt ggtacaccac agggaggaaa tttatctcct ctcttatcca
     1261 atatcatgct taatgaattg gacaaggaat tagaaaagag gggacttcga tttgtgcgct
     1321 acgcagatga ttgtgtgatt acggtcggaa gcgaggcagc ctctaagcgt gtgatgtatt
     1381 cagtcagtcg ttttattgag aaacggctag gtttgaaagt aaacatgacc aagactaaga
     1441 ttaccagacc aagagagttg aaatatctag gttttgggtt ctggaaatca tcagatggtt
     1501 ggaaaagccg tccacatcaa gatagtgttc ggagatttaa gtttaaattg aagaaactaa
     1561 cacagaggaa atggagtatt gagcaactga atttgtctat tcgaggatgg ataaactatt
     1621 tctcattggg aaatatgaaa agtatagtcg ccagcataga tgagcgcttg cgtactcgcc
     1681 tacgagtgat tatctggaag caatggaaga agaaatcgag acgattatgg ggattgctta
     1741 agttaggagt tcctaaatgg atagcagata aggtatctgg ctggggcgac cattatcaat
     1801 tagtagctca gaagtcggta cttaaacgtg ctatatcaaa accagtcctg gaaaaacgtg
     1861 gactggtttc gtgtttggat tattaccttg aacgacatgc gttaaaagtt agttgaaccg
     1921 ccgtatgccg aacggcacgt acggtggtgt gagaggggct agagattatc ccctactcga
     1981 ttaactcccc tgaaatttat tttaattatg caaatttcac gtatttttga tgctgagacg
     2041 acgatcctgg gaacttttca gatatttttt tgactatcta aatctatcat tagaaaagct
     2101 tagagcgcca aaggatttga gcgtttttct gatttttaag actttttcca gtctcttttt
     2161 cgattgaaga tgtaattatt ctactaacta actaacttct tagtactagc caacaacgat
     2221 aatcataatt cctcctaaaa ttaggaataa taaaggcaat agtttttgtt ttttcatgta
     2281 aaaaacctca cttttgtttt ctgctatttt atgctaaaat attaaaaatc aaatttaatt
     2341 ccaaagtttg taactaaagg gggagcgcta catgtctaat ttatttgtca agttgttagt
     2401 ctctcaatta tttgcaaatt tagcagatat tttctttaga gtaacaatca ttgctaacat
     2461 atacattatt tcaaaatcag taattgccac atcactagtt cctatcttaa taggaatatc
     2521 ctcttttgtt gcgagtcttt tagttccgtt ggttactaaa aggttagcgc taaatagggt
     2581 tttatcttta tctcaatttg gaaagactat attattggcg atactggtag gaatgtttac
     2641 cgtaatgcaa tccgtagcgc ctttggtgac ctatctattt gttgttgcaa tttccatact
     2701 agatggtttt acagcacccg tttcctatgc tattgtgcca cgctatgcga ccgatttggg
     2761 taaggctaat tcagccttat caatgactgg tgaagctgtt caattgatag gttggggatt
     2821 aggtggactc ttgtttgcaa caattggtct gttacctacc acgtttatca ttttagtctt
     2881 gtatatcatt tctagctttc tgatgttatt tcttcctaac gctgaagtgg aggtgttaga
     2941 gtcagaaact aatcttgaaa ttttgctcaa aggttggaag ttagttgcta gaaatcctag
     3001 attaagactt tttgtatcag caaatttatt ggaaatttct tcaaatacga tttgggtttc
     3061 ttccattata cttgtttttg taacggagtt attaaataaa acggaaagtt actggggata
     3121 ttctaataca gcatactcta ttggtattat aattagtggc ttaattgctt ttaggctatc
     3181 tgaaaagttc cttgctgcta aatgggaaag tattcttttt cctcttgtag ctatggcaat
     3241 agtgacactg actattctat attttccaaa cgcacagatg tttttattat tttcagcttt
     3301 agttggtatg ttatcgcaac taaaagaagt tcctgaaagt gtatttcttc aggaaacagt
     3361 agaggaaaat catttagtta atgtctattc cgttcttgaa gtgatttcta cattagcatt
     3421 ttcagtattt gttttgctaa tgagctatat tactgagagt tttggtatta gcatcagttt
     3481 ttggctatca gccatttgtc tgatgataga agctatttta atttatatca gacgagatta
     3541 ttttaaatga gagagtggtt aaaaagtctt taaaaatcag aaaagcgcat agtatcaggt
     3601 gttgaaaaaa cttgatattg tgtgttttat tatgggaaga tttacttcat tgtctcctga
     3661 aatggagttt tgaagcagga tcactctatt tgaaaatata catagaaaaa ctgtataatc
     3721 cttaaaataa gctgaaatct attggaaaca tacggtaaac aattgaaaag atactatata
     3781 aagtggtaca atggtagaaa aataagctag taagaatcac tcttatttta gcgaaaaatt
     3841 acagaaatga atggtttttc ttgatgaaac agagaaaaga attgtacctc tttcttggtc
     3901 ggacagcctt gtattttctt atctttctag ggctgcttta cttctttagc tatcttggtc
     3961 agggtcaagg aagctttatc tataatgaat tttaacttga aggagaatcc ctattgtgtc
     4021 aaataaacca atagcagata tgattgaaac cattgagcat tttgctcaga cacagcctag
     4081 ctatcctgtc tataatgttt tggggcagga acacacttat ggcgatttaa aggctgattc
     4141 ggatagtttg gctgcagtca ttgaccaact aggcttgcct gagaagtctc ctgtggttgt
     4201 ttttggtggc caagaatatg aaatgttggc aacctttgta gcgctgacta agtcaggtca
     4261 tgcctacatt ccaattgata gccattcggc cttggagcga gtttcagcta ttttagaagt
     4321 agcagagcca agcttgatta ttgccatttc agcctttccc ttggagcagg tttctacacc
     4381 aatgataaat ctagctcagg ttcaagaagc ctttgcccaa gggaacaact atgaaatcac
     4441 gcatccagtc aagggagatg ataattacta cattatcttt acttctggta cgactggtaa
     4501 gcctaaggga gtgcagattt cacatgataa tctcctcagc tttacaaact ggatgattac
     4561 ggataaggaa tttgcgacac cgagtcgtcc gcaaatgctg gcacagccac cttattcttt
     4621 tgacttgtct gtcatgtatt gggcaccgac cttggcactt ggtggtacgc ttttcactct
     4681 tccttcagtc atcactcagg actttaagca actctttgcg gctatctttt cattgccaat
     4741 cgctatctgg acatcaacac catcctttgc agatatggcc atgttgtctg aatacttcaa
     4801 cagtgagaaa atgcctggaa tcacgcattt ctactttgat ggtgaagaat tgacggtcaa
     4861 aacagctcaa aaactgcgcg agcgtttccc aaatgcccgt atcatcaatg cttacggccc
     4921 aacagaagcg acagtagctt tgtcagcagt tgccgtgaca gacgagatgt tagcgactct
     4981 caaacgccta ccaatcggct ataccaaggc tgattctcca acctttatca ttgacgagga
     5041 aggaaataaa ctgccaaatg gtgagcaggg agaaatcatt gtttctgggc cagctgtttc
     5101 aaaaggttat atgaaaaatc ctgaaaaaac agcagaagcc ttctttgagt ttgaaggtct
     5161 gccagcctat cacacaggcg atgtgggaac catgacagat gagggcttgc ttctctacgg
     5221 cggacgcatg gacttccaga ttaagtttaa cggttaccgc attgagttag aagatgtctc
     5281 tcaaaacctc aacaagtctc gctttatcga atctgctgtc gcagtaccgc gctataacaa
     5341 ggaccacaag gtacaaaatc tattggctta tgtcatctta aaagacggtg ttcgtgagca
     5401 gtttgagcga gatatcgata ttaccaaggc catcaaggaa gacctgacag acatcatgat
     5461 gtcctatatg atgccatcta aattccttta ccgagacagt ttgccactaa ctccaaatgg
     5521 aaagattgac atcaaaggat tgattaacga ggtgaataag agatgatgga gttttttcaa
     5581 cagcttcctc atttagagcc atatggcaat cctcagtatt ttgtttatgt gattgctgca
     5641 accttgccca tctttatagg tctctttttc aagaaacgct ttgcctggta tgaagtgttg
     5701 gtaagtctct tctttattgt caccatgttg gtgggtggaa agaccaatca actagctgcc
     5761 ttgggtattt acctttgctg ggaaatattg ctcctgcttt tctacaagca ttatcgaaaa
     5821 agcaaggatg gcaagtgggt cttctactta gttagttttc tgtccctact tccgattatc
     5881 tttgtcaagg tgcaaccagc tatcaatgga acgcagtctt tgcttgggtt cttgggaatt
     5941 tcttacctga cctttcgttc ggttgggatt atcatcgagc tgagagatgg agtgattaag
     6001 gattttaccc tctgggaatt cctccgtttc cttctcttca tgccaacttt ctcgagtggt
     6061 ccaatcgatc gctttaagcg atttaatgaa aattatcagg ctattcctga gcgagatgag
     6121 ttgatggata tgctggatga atctgtccgc tatatcatgc ggggcttttt gtataagttt
     6181 atcctagctc atgttttagg agagacctta ctacctcctc tgaagaattt agccttgcag
     6241 tcaggtggct tctttaatct ctatgccttg gcagttatgt atacttttgg tctggagctc
     6301 ttctttgact ttgcaggtta ttctatgttt gctttggcca tctcaaactt gatgggaatc
     6361 cgtagcccta tcaactttaa caagcccttt ttatcaaggg atttaaagga gttttggaat
     6421 cgctggcata tgagtctgtc cttctggttc cgtgactttg tctttatgcg aatggtgatg
     6481 gtgttaacca gaaagaaagt ctttaaaaat cgtaatgtaa cctcaagcat ggcctacatt
     6541 gtaaatatgc tgattatggg attttggcat ggcgtgacct ggtactatat cgcctatgga
     6601 ctctttcatg gactaggctt ggtcatcaat gatgcctggg ttcgcaagaa aaaaacgctc
     6661 aataaggaac ggaaaaaagc agggaaggct gccctacctg agaatcgctg gattcagttg
     6721 cttggcatgg ttgtcacttt ccatgttgtc atgttgtcat tcttaatctt ttctggattc
     6781 ttgaataatc tatggtttaa aaaataaaag gaaataaaat atggatatca aatcagaagt
     6841 tattgaaatt attgatgagt tgtttatgga agatgtttct gacatgatgg atgaagatct
     6901 ttttgatgca ggtgtcttgg atagtatggg aacggttgag ttgattgtgg agattgagaa
     6961 ccgttttgac attcgtgtcc ctgtaacaga gtttggtcgt gacgactgga atacagctaa
     7021 taaaatcata gctggtattg tggagctaca aaatgcttaa acgcttatgg atgatcttcg
     7081 gaccggtctt gatcgctggt ttgttggttt ttctgctcat tttcttttat cctactgaga
     7141 tgcatcataa tctaggagct gaaaagcgtt cagcagtggc tactactatc gatagtttta
     7201 aggagcgaag tcaaaaagtc agagcactat ctgatccaaa tgtgcgtttt gttcccttct
     7261 ttggctctag tgaatggctt cgttttgacg gtgctcatcc tgcggtatta gctgagaaat
     7321 acaatcgttc ctaccgtcct tatcttttag gacagggggg agctgcatcg cttaaccaat
     7381 attttggaat gcaacagatg ttaccacagc tggagaataa acaagttgtg tatgttatct
     7441 cacctcagtg gttcagtaaa aatggctatg atccagcagc cttccagcag tattttaatg
     7501 gagaccagtt gactagtttt ctgaaacatc aatctgggga tcaggctagt caatatgcag
     7561 cgactcgctt actgcaacag ttcccaaacg tagctatgaa ggacctggtt cagaagttgg
     7621 caagtaaaga agaattgtcg acagcagaca atgaaatgat tgaattattg gctcgtttta
     7681 atgaacgcca agcctccttt tttggtcagt tttcggttag aggctatgtt aactacgata
     7741 agcatgtagc taagtattta aaaatcttgc cagaccagtt ttcttatcaa gcaatagaag
     7801 atgttgtcaa agcagatgct gaaaaaaata cttccaataa tgagatggga atggaaaatt
     7861 atttctataa tgagcagatc aagaaggatt tgaagaaatt aaaggattct cagaaaagct
     7921 ttacctatct caagtcgcca gagtataatg acttgcagtt ggttttaaca cagttttcta
     7981 aatctaaggt aaacccgatt tttatcattc cacctgttaa taaaaaatgg atggactatg
     8041 ctggtctacg agaggatatg taccaacaaa cggtgcagaa gattcgctac cagttagaaa
     8101 gtcaaggttt taccaatata gcagattttt ctaaggacgg cggggagcct ttctttatga
     8161 aggacaccat tcaccttggt tggttgggtt ggttggcttt tgacaaggca gttgatcctt
     8221 tcctatccaa tcccacacca gctccgactt accatctgaa tgagcgcttt ttcagcaaag
     8281 attgggcgac ttatgatgga gatgtcaaag aatttcaata gataatataa tatagaagag
     8341 ttcaagaatg aaactagttt tcacgttttt tcttgactct ttttttgctt gtgatataat
     8401 taactggtaa acaaaatgtc aaagaatagt atttttctct taaaaaagag aagtctaaaa
     8461 ggaaaggagt cagatatgag acagctagca aaggatatca atgctttttt gaatgaggtg
     8521 attttgcagg cggaaaatca gcatgaaatc ctaataggtc attgcactag cgaggtggcc
     8581 ctgaccaata ctcaggagca tatccttatg ctcttgtcag aggaatcttt aacaaattca
     8641 gaattggccc gtcgtctcaa tgtcagtcag gcggcagtta ccaaggccat taagtctttg
     8701 gtcaaggaag ggatgttgga aacatctaaa gattctaaag atgcgcgtgt gattttttat
     8761 cagttgactg acttggctcg tccaatcgct gaggagcacc accatcacca tgagcataca
     8821 cttttaacct atgaacaagt ggcgactcag tttactccaa atgaacaaaa agtgattcag
     8881 cggtttttga ctgctttagt aggagaaatc aaataatgag atatattacg gtagaggatt
     8941 tgtccttcta ttatgataag gagcctgttc ttgaacatat caattattgt gttgatagtg
     9001 gggaatttgt taccttgact ggggaaaatg gagcggctaa gacgacgctc atcaaggcta
     9061 gtcttggaat tctgcaacca cgcattggaa aggtggctat ttcaaagaca aatacgcaag
     9121 gtaagaaatt gagaatagcc tatcttcctc aacaaattgc cagttttaat gctggttttc
     9181 caagtacggt ctatgaattt gtcaagtcgg gtcgctatcc gagaaaaggc tggttccgtc
     9241 gtttgaatgc tcatgatgag gagcatatca aggctagtct ggactcagtt ggcatgtggg
     9301 aacatcgaga caaacgcttg gggtctctat ctgggggaca aaagcagcga gcggtgattg
     9361 cgcgtatgtt tgcttctgac cctgatgtgt ttatcctaga cgagccgaca acggggatgg
     9421 atgcaggaag taaaaatgaa ttttacgaac tcatgcacca cagcgcccat catcatggca
     9481 aggctgtttt gatgattacc catgaccctg aagaagttaa ggattatgcg gatcgcaata
     9541 ttcatctagt ccgtaaccaa gactcgccat ggcgctgttt caacgttcat gagaatggcc
     9601 aggaggtggg tcatgcttag tttattatct tacgacttta tacaacgcgc ctttttggcg
     9661 gttattgcta tgagtctttt ctcaccggta ttgggaacct tcctcatctt gcgtcgtcag
     9721 agtttgatga gtgataccct tagccacgtc tcactttcag gtgtagcctt tggtctggtt
     9781 ttggggattt ctccaactgt ttctactatt gccattgtct tgattgcggc ggtctttctg
     9841 gagtatctcc gtacggttta caagagcttt atggaaatcg ggacagctat cctcatgtca
     9901 acaggtctgg ctgtttctct gattgtcatg agcaagggta aaagctcgag ttcaatgagt
     9961 ttggaccaat atctctttgg ttcgatcgtg actatcagtg aagaacaggt catttccctc
    10021 tttgtcattg cggcggttgt tttgattttg acctttctct ttcttcgtcc tatgtatatc
    10081 ttaacttttg acgaagatac ggcctttgtg gatggcttgc cagttcgtac catgtccatt
    10141 ctttttaaca tggtgacagg ggtggctatt gcccttatga ttcctgcagc aggagctctt
    10201 ctggtatcga ccattatggt cttgccagct agtattgccc tgcgtctggg gaaaaacttt
    10261 aaatcggtta tgctgcttgc cagtgcgatt ggctttttgg gaatggtagc aggactttat
    10321 atttcctact atgcagaaac acctgcaagt gcaagtatta ccattatttt tgtaactgtc
    10381 tttatactaa tcagtttagt aagacgtttt atcaaatagg agacgaacgt gaaaaaaatt
    10441 agcttattac tagccagtct atgtgccttg tttttagtgg cttgttccaa tcaaaaacag
    10501 gcagatggta aactcaatat cgtgacaacc ttttaccctg tctatgaatt taccaagcaa
    10561 gtcgcaggag atacggctaa tgtagaactc ctaatcggtg ctgggacaga acctcatgaa
    10621 tacgaaccat ctgccaaggc agttgccaaa atccaagatg cagatacctt cgtttatgaa
    10681 aatgaaaaca tggaaacatg ggtacctaaa ttgctagata ccttggataa gaaaaaagtg
    10741 aaaaccatca aggcgacagg cgatatgttg ctcttgccag gtggcgagga agaagaggga
    10801 gaccatgacc atggagaaga aggtcatcac catgagtttg acccccatgt ttggttatca
    10861 ccagttcgtg ccattaaact agtagagcac atccgcgaca gcttgtcagc agattatcct
    10921 gataaaaaag agacctttga gaagaatgca gctgcctata tcgaaaaatt gcaagccttg
    10981 gataaggctt acgcagaagg cttgtctcaa gcaaaacaaa agagctttgt gactcaacac
    11041 gcagccttta actatcttgc cttggactat ggactcaaac aagtcgcaat ctcaggactt
    11101 tctccagatg cagagccatc agctgctcgc ttggcagaat tgacagagta tgtcaagaaa
    11161 aataaaatcg cctatatcta ttttgaagaa aatgcctcac aagcccttgc taacacactt
    11221 tcaaaagaag caggtgtcaa aactgatgtc ctcaatcctt tagaaagtct gacagaagag
    11281 gacaccaagg ctggagaaaa ctacatttcc gtgatggaga aaaacctcaa ggctttgaaa
    11341 caaacaacag accaagaagg cccagcaatc gaacctgaaa aggcagagga taccaagaca
    11401 gtccaaaatg gttacttcga ggatgcagct gtcaaggacc gcaccttgag tgactatgca
    11461 ggtaactggc aatcagttta tcctttcctt gaagacggca cgtttgacca agtctttgac
    11521 tacaaggcta agttgactgg taagatgacc caggctgagt acaaggctta ctatacaaaa
    11581 ggctatcaga cagatgtgac taagattaac attactgata atactatgga atttgttcaa
    11641 ggtggacaaa gcaagaaata cacttacaag tatgtcggta agaaaatttt gacttacaag
    11701 aaaggcaatc gtggcgtgcg tttcctcttt gaagccacag atgctgacgc tggacaattc
    11761 aagtatgttc agtttagtga ccacaatatc gccccagtta aggcagaaca tttccatatc
    11821 ttttttggag gtacaagcca agaaactcta tttgaagaaa tggataattg gccaacctac
    11881 tacccagata acctatctgg ccaagaaatc gcccaagaaa tgttggcgca ttgatgagaa
    11941 atcgactagt tcataagagc tagtcggttt tttgatacta gatggctaac ttcattatag
    12001 acctttcaga actaagggaa cctgaaaaaa taaataaaac tcttgaaata tctgggctta
    12061 tatgctagaa tgtagttagt gtataaggtt cattagaaca ccaaagcccc taactagtgc
    12121 catagttaag ggcttttttg acgtatctta gcgatttaac gggtgtttat aggctagtca
    12181 ttcggtctac ttcttaccat ctagccattt gcagacgaaa tacaagataa ttccagctat
    12241 aacatctgct ataatagtaa gagcaagctc tggtataagg ctcattagtt tcacctcctc
    12301 tcacgaaccc ataggaacgt aatcggtaac cgatgacaaa aatagtatac cacaatacat
    12361 ttagatcatc aaggtcactt aattcttgaa ctattgtaga ttatatgcta aaatgtagtt
    12421 aattgataag gttcattaga acaccaaagc ccctaactat ggccgtagtt aagggctttt
    12481 ttgacgtatc ttagcgattt aacgggtgtt tataggctag tcactcggtc tacttcttac
    12541 tatctagcca tctgcagacg aaatacaaga taattccagc tataacatcc gctataatag
    12601 taagagcgag ctctatgata aggctcatta gtttcacctc ctctcacgaa cccataggaa
    12661 cgtaatcggt aaccgatgac aaaaatagta taccacaata catttagatc atcaaggtca
    12721 cttaattctt gaaatatagg gtctaggcga caaagtcttt aaaatcagaa aaacgcataa
    12781 tatcaggtgt gcaaaaactt gatactatgc gttttattgt gggaaggttt actccatttt
    12841 ctcctgaaat tgagtttttg tccagcctct gtttttaggg ttgctgagaa aataatgtca
    12901 tgtggtgaat atttgtaaat cagtcagcag acagaacgaa tactcttcga aaatctcttc
    12961 acatcatgtc agcttcgtct ttccgtatat atgtgactga cttcatcagt tctatctaca
    13021 acctcaaaac agtgtttcga gctgacttca tcagttttca aatctgtact ttgagcaagc
    13081 tgagactagc ttcctatttg attttcattg aatatcagaa acccattctc catcaaataa
    13141 ttcgactgcg tctaataatt tttgatctgg cacggtgtct gaaataaagg ttgtgtattt
    13201 ggagagggga ttaattttaa aaaatccagt cttgtaaaat ttagaactat caatcagtaa
    13261 gatggtttca tgggctttgt caataatatt cttttttgaa atagcttggc tgagagaagc
    13321 ttcataaaca tattggtcat caatacctct tgctgaacaa aatgctaaat cgatattaaa
    13381 atgatctaat aaagaatttt ccttatcata gttgaccacg gaacaggatt gatgtttgac
    13441 ctcgccagat gtgataaaga ttttggagct atctttaaca gtttcagata gggtttgtgc
    13501 agtatgtaaa ccatttgtaa aaataatcaa attatcaagt tcagaaagat agggacaaag
    13561 ttcgtagaca gtagtactag aatctagata gatacacata ccagaccgaa taaagtcttt
    13621 agcgagacta gcgattagtc ttttttgcct agtactttct ccttcccgta tttgatgaga
    13681 aagttcaatt gtgttcatag aggacagggt cacgtatccg tgctttcttt tgataagacc
    13741 ttgattttct aagaaaatta aatcgcgacg taaggtactt gtgctggaga aagtgatttc
    13801 tgccagctct tttacggcaa ttcttttttt ctttttgata atttcaatca attcaagtac
    13861 acgttcatct tttatcataa gctcctccta atttatcatt tcaactatat tatagcacaa
    13921 attggaggaa tttgaattat ttttatgaat attgggttaa catttgaaca ttattcaagt
    13981 aagcgttcac atattgaaaa aataaaacgt ggggattata ataaagttaa tcaaggacga
    14041 agagagaaga aaaatggaag cggttttagc aatagattta ggtgcgactt ctggaagagc
    14101 aatcgttggt tacctttctg aaaataaact agtaatggaa gaaataaatc gcttttctaa
    14161 tctacctatt agagtaaaag ggcatttatc ttgggatatt gactttctac tagctaaaat
    14221 tcttgaaagt atccgcttgg ctaatactag ttacaagatt ttatctatcg gtattgacac
    14281 atggggagtt gattttggac tgattgataa tgaaggtaag ctgttattac aacctgttca
    14341 ttatcgtgat gaaagaacaa agggagtgtt aaaggaaata tctgaaatga ctgaattaga
    14401 aaaactgtat tcagagacag gaaatcagat tatggagata aataccttgt ttcaactctt
    14461 taaggcacgt caagaatctc ctgactcttt ctataagacc aataagattc ttttaatgcc
    14521 agatttgttt aattatctct tgacaggtaa gtttgctaca gaaaaaagca ttgcttcaac
    14581 aactcaatta tttgatccta ggagtcaaaa ctggaatcag aatatcttaa aactatttga
    14641 attggattca tctttacttc ctgagattgt ttcagaggga aatgttcttg gaaggataaa
    14701 aaaggagtat ggtttaggcg atattcctgt tgtgaatgtt tgtagtcatg atacagcaag
    14761 tgcgattgtc tcagtaccta agacagaagg tagtttattt atttcatcag gtacttggtc
    14821 tttggttgga gtggaactta cttcaccgat tcttactacc gaatccttca gttatggatt
    14881 tacaaatgaa gtcggtaaag atggagtgat tacatttctg aagaattgta cagggttgtg
    14941 gatcatagag gaactaagac gttcatttga acgaagaggg aaagcctatt cttttgatga
    15001 tattaggaca atggtggaga aagaaaaaga aaatcttcct ctgattgata ctgaatcaac
    15061 tgaatttgca acagaatctg atatgcacaa gactttgaca gaatatctag cttatcatca
    15121 tgaaactaga gagtggacag atggacaact atttaagatt gtttatgaaa gcctagctga
    15181 aacgtatagg aaagcgatag agttattaga agaactaact cataaggttt ataagaggat
    15241 atatgtgatt ggaggaggtg ctagagccag ttattttaac caaatgattg ctgatagaac
    15301 tggtaaagag gttcttacag gttcgactga gggtacagct gtggggaata ttgttgtgca
    15361 gctcatagct atgggacaat taaaagggat ggaagaagct caccatgtta ttgaggagtt
    15421 tctacaatta gagagttatt actcccaaaa gaattaaaaa gattgagagt ttgtaaattt
    15481 gcctccctcc cccttcttaa gttttgtaca agaagggggg ataattggtg aattgaaaaa
    15541 tatttagtgt tttgatatga ggaggacaag aatgtcagat gtaaaacaag aattaattaa
    15601 gtatggcaag aagctagtag aaacagattt gacgaaagga acaggtggga atctcagtgt
    15661 tttcgatcgt gaaaaacaat tgatggcaat taccccgtcg ggtattgatt tctttgaaat
    15721 caaagaatcc gatattgtag tgatggatat taatggaaat gttgtagagg gagaacgctt
    15781 gccatctagc gaatggtata tgcatttgat tcaatatcaa actcgtgatg atatcgatgc
    15841 aattatccat gctcatacaa cttatgcaac agtattagct tgtctcagag aaccacttcc
    15901 ggctagtcat tatatgattg cagtggcagg gaaagatgtt cgggtagctg agtatgcaac
    15961 atatggtacg aaagaattgg ctgtgaatgc agctaaagca atggaaggtc gtagagcagt
    16021 tttactagcg aatcatggaa ttttagcagg tgcacaaaat ttattgaatg catttaatat
    16081 tgttgaagaa gttgaatatt gtgcaaaaat ttattgttta gctaagaatt ttggagagcc
    16141 agtagttctt cctgatgagg agatggattt aatggcagaa aaatttaaaa catacggtca
    16201 gagaaaatag ggaggatatt aatgttaaaa catataccga aaaatatttc tccagattta
    16261 ttgaagactt taatggaaat gggacatgga gatgaaatag tattagctga cgcgaattat
    16321 ccttctgcct catgtgcaaa taagctaatt cgttgtgatg gtgtaaatat tccagaatta
    16381 ttagattcca ttctgtattt aatgccatta gatagttacg tcgatagttc aattcagttt
    16441 atgaacgttg tttcgggtga tgatattcct aagatatggg gtacctatag acagatgatt
    16501 gaaggtcatg gtacagatct taaaacgatt acttatctta gaagagaaga cttttatgaa
    16561 cgtagtaaga aagcttatgc tattgttgct acaggagaaa cttcacttta tgctaatatt
    16621 atccttaaga aaggagtagt tgttgaaaga gaaaatgttt aatagaggaa ttttagttgc
    16681 cagtcatggt aattttgcta gcggagctct catgaccgca gaaatgtttg ttggtgagac
    16741 aacaaatgat agagttagga cattaggttt gatgcctgga gagaatattg tagagtttga
    16801 acattatttt aaaaatcaag taaatgaact gttagactca aatcaagagg ttatcgtttt
    16861 gactgacttg attggaggaa gtcctaataa tgtggctttg tcacggtttt taaatttgga
    16921 ttcagttgat attgtaacag ggtttaatat ccctctccta gtggaattaa tatcaagtta
    16981 tgattcaaaa atcaatttag aagaaattgt tcaaaatgct caaaatagtt tgtttaatgt
    17041 taaacaacaa cttaacgtag aggaggaaga ggatttatgt ctatagagtt tgttcgtatt
    17101 gatgaccgtc tggtacatgg tcaagttgtc actacgtggc taaaaaagta tgatattgag
    17161 caagttatca ttgttaatga tcgcatctca gaagataaaa cacgacaatc tattttaaag
    17221 atttctgcac cggtaggttt aaaaattgtt ttctttagtg taaaacggtt tgtggaagtt
    17281 ttaaactctg tgccaataaa aaagagaaca atgctgatat atacaaatcc aaaagatgtg
    17341 tatgattcta ttgaaggaaa tttaaaattg gagtacctca atgtaggaca gatgagtaaa
    17401 acggaggaaa atgaaaaggt aacgggaggt gtagctctag gtgaagaaga caaatattat
    17461 tttaagaaaa tagttgataa gggaacgaga gttgaaattc aaatggttcc taatgataaa
    17521 gttacaatgt tggaaaaatt tttataaaaa taatttaagg aggtacagta tatgctattc
    17581 acacaagcat tactggtgac attagttggg attattgcca ctattgacta taatggaccg
    17641 ttatttatga ttcaccgtcc gttagttaca agtgcaatgg ttggcttagt cttaggagat
    17701 ttcacccaag gtgttcttat tggttcagct cttgaattaa cttggctcgg tgtaacaggt
    17761 attggaggtt acactccacc agatactatt tcaggtgcga ttattggtac tgcatttggt
    17821 attttgtctg gtcaaggaga aactgctggt atcgctatag cagttccaat tgcagttgct
    17881 acccaacagt tggatgttct tgcaaaaact ttagatgttt attttgtgaa aaaagctgat
    17941 aatgatgcta aaaacggaga ttattcaaag atcggttttt atcattattc aagtttggtt
    18001 ttaatcactt tatttaaaat tgtaccaatt ttcctagcta ttatgcttgg aggggaatat
    18061 gtggcagact tgtttgctaa ggttccacca atcgttatgc agggacttaa ctctgcaggt
    18121 gctttactac cttcaattgg ttttggtatg cttttaaata tgatgctcaa gaaaaatatg
    18181 tgggtattct tgttgattgg attcatttgt tctgtgtatg gaggaatgtc aaccattggg
    18241 atctcactag ttggtattgc ggtagcatac ttctacgata tgattggaag taaaccacaa
    18301 gaaacaactt caagtagtga tgttgaggag gatcttgatc tatgatgaat aataaagtaa
    18361 ctaaagttga acttaaaaaa gttttcaaac gaagttttat gtatggttct tcatggaact
    18421 atgagagaat gcagaaccta ggttttctat atacaattct tccagtattg aaaaaactat
    18481 acccagacaa agattcagct tctcctgcaa tgaaacgtca ccttgagttt ttcaatactc
    18541 atcaaacagc ggcaccattt attcttggag ttacttccgc tatggaagaa caagaaggaa
    18601 atgaaggtgc agcttcaatt actggtatta aagttggctt gatggggccg ctggctggtc
    18661 taggagatag tttgttctgg ctgacactag ttcctatctg ttttagtatt ggtgcgtctt
    18721 attctaaaga cggcggtgct ttaggtatct ttatcgcctt aatattgttt aatattatta
    18781 atattcctgt taaatatttc ggtttgaaat atgggtatac taagggttct agtcttatcc
    18841 aagaaaataa tacaaaagga acattgaatc gcgttacgag tatggcgaca gcattagggc
    18901 tagtactagt gggtggtttg attccatcaa tggttggtat taattttgga ttagaattta
    18961 agcaggggga acttgttatt tctgttcaag aaatgattac aaaattaatt ccaggattta
    19021 tccctatggc tttgacttta ttaatgtgta aattaattag aaaaggaaag aatccggttg
    19081 tactaatctt tagtgttatg gctattggag ttatcctagt tgttttagga attttgaagt
    19141 agtagaaagt gtggaggtgg tatttgggat atcacctcca ttttggaaga gaggtaaaga
    19201 gtgaaattat ggtataagaa agctgcctca aattggaatg aagccttgcc gattgggaac
    19261 ggtcatttag gtggtatgat ttatggttca gctacaaaag aatgtattca actaaacgat
    19321 gagactattt ggtatagagg aaagtcagat agaaataatc cagactcact attgcatctt
    19381 aaaaaaattc gggaatatct tttagatgga gaaattcaga aagccgaaga attgataaag
    19441 ttaacaatgt ttgctacccc aagagatcaa agccactatg aattacttgg ggaactttac
    19501 attgagcata tagatattca gtcttgtgct ctttcattgt atgaaagaga gctagattta
    19561 gatacagcta tttctaatgt tgtgtttgag cctaatagtt gtaatttaca aataaaaaga
    19621 gaatatttta cgagttttaa taagaatatt ttatgttgcc gtatagtgtc atcagttcaa
    19681 aacacattaa atttaaacat taatttgggt agaaataaac ggtttaatga cgaagtatct
    19741 aaactggatt caagtacaat tttaatgtcg gcctctgctg gaggtagaaa aggtgttcag
    19801 tttaaagtag tatgtcattc taaagttacg gatggtgaag taagtgtatt gggagagaca
    19861 atagttattc ggaatgctac agaggtattt ctttatctca aatcaatgac ggattattgg
    19921 ggaaatatag atatttcttc tcttcaggga gaatttagta gtattgatta ctttacagaa
    19981 aaagatgaac atgtaaaaaa atatcaggag caatttaata gagttgattt taaactagac
    20041 tatagtaaag attgtcttag cattccaacg aatctacttc ttgaaaacac taaaaagtat
    20101 agtaactact tgactaactt gttatttcat tatggaagat atctgttaat atcgtctagt
    20161 caaccgaatg gtttacctgc caatcttcaa ggaatatggt gtgatgaatt aaatccaatt
    20221 tggggttcta aatatacgat taatattaat actcaaatga attattggat ggtaggtcca
    20281 tgtgatttac cagaagtaga atatccatta tttgatatgc tcgaaagaat gagagaaccg
    20341 ggaagactaa ccgctaagaa aatgtatgga gctagaggtt ttacagcaca tcataatacg
    20401 gatggttttg gcgatacggc tccccaatct catgccatgg gggctgcaat ttgggtatta
    20461 actattccat ggttatgtac tcatatttgg gaacactatt tatatttcca agatgagcgt
    20521 attcttacgg aacattttga aatgataaaa gaagcatttc ttttctttga agattattta
    20581 tttgaggtgg atggctactt gatgacaggt ccaagtgtct caccagaaaa taaatatcgc
    20641 ttaaaaaatg gtattgaagg aaatgcttgt ctatcatcta caattgataa tcaaattcta
    20701 agatattttt gtgattcatg cattggcatt gcaaaacaat taggagacaa ttcggatttt
    20761 attagtcgtg tgaaggagtt aaaaaagaaa ctacctaaaa caaaaatagg tagtaatggg
    20821 caaatccaag aatggttaga agattatgaa gaagtagagc ctgggcatag acacatttca
    20881 cctctatttg ggctttatcc ttataatgag attgatattc ataaaactcc ggaattagca
    20941 gaagcagcta aaatcactat caataggaga ttatcaaacg ctaacttttt atcttcacag
    21001 gagagggagc aagcgattaa taattggtta gtaagtggtt tgcatgctag tacacaaaca
    21061 ggttggagtg ctgcatggct gattcatttt tttgcgagac tatatcaagg tgaacctgct
    21121 tataaccaga ttaatggttt gttaaataat gcgactcttg gcaatttatt tcttgaccat
    21181 ccaccatttc aaattgatgg taatttaggt ttggtgagtg gaatttgtga attattagta
    21241 cagagccatc ataattggtt atcactaatt ccagctttac cttctgcttg gtcagaagga
    21301 gaagtgaaag gtttcagagt aagaggagga tataaggtat cgtttgcttg gaaaaatggg
    21361 gatataacat tcctaaaatt ggaaggagga aacaaagatc aaaaagtaag agtaagaata
    21421 tatggcaaaa atactgatgt acaaaatatt gaattggtat ttaattcaga aaaaattatt
    21481 gagttaaatt tttaggtata agtcatgaat aaagaaaaaa taaaaagaaa attaatcaca
    21541 atactgtttg tatgtattgg gatgttatgt tttggattgt tagcaggagt taaggctgat
    21601 aatcgtgttc aaatgagaac gacgattaat aatgaatcgc cattgttgct ttctccgttg
    21661 tatggcaatg ataatggtaa cggattatgg tgggggaaca cattgaaggg agcatgggaa
    21721 gctattcctg aagatgtaaa accatatgca gcgattgaac ttcatcctgc aaaagtctgt
    21781 aaaccaacaa gttgtattcc acgagatacg aaagaattga gagaatggta tgtcaagatg
    21841 ttggaggaag ctcaaagtct aaacattcca gttttcttgg ttattatgtc ggctggagag
    21901 cgtaatacag ttcctccaga gtggttagat gaacaattcc aaaagtatag tgtgttaaaa
    21961 ggtgttttaa atattgagaa ttattggatt tacaataacc agttagctcc gcatagtgct
    22021 aaatatttgg aagtttgtgc caaatatgga gcgcatttta tctggcatga tcatgaaaaa
    22081 tggttctggg aaactattat gaatgatccg acattctttg aagcgagtca aaaatatcat
    22141 aaaaatttgg tgttggcaac taaaaatacg ccaataagag atgatgcggg tacagattct
    22201 atcgttagtg gattttggtt gagtggctta tgtgataact ggggctcatc aacagataca
    22261 tggaaatggt gggaaaaaca ttatacaaac acatttgaaa ctggaagagc tagggatatg
    22321 agatcctatg catcggaacc agaatcaatg attgctatgg aaatgatgaa tgtatatact
    22381 gggggaggca cagtttataa tttcgaatgt gccgcgtata catttatgac aaatgatgta
    22441 ccaactccag catttactaa aggtattatt cctttcttta gacatgctat acaaaatcca
    22501 gctccaagta aggaagaagt tgtaaataga acaaaagctg tattttggaa tggagaaggt
    22561 aggattagtt cattaaacgg attttatcaa ggactttatt cgaatgatga aacaatgcct
    22621 ttatataata atgggagata tcatattctt cctgtaatac atgagaaaat tgataaggaa
    22681 aagatttcat ctatattccc taatgcaaaa attttgacta aaaatagtga ggaattgtct
    22741 agtaaagtca actatttaaa ctcgctttat ccaaaacttt atgaaggaga tgggtatgct
    22801 cagcgtgtag gtaattcctg gtatatttat aatagtaatg ctaatatcaa taaaaatcag
    22861 caagtaatgt tgcctatgta tactaataat acaaagtcgt tatcgttaga tttgacgcca
    22921 catacttacg ctgttgttaa agaaaatcca aataatttac atattttatt gaacaattac
    22981 aggacagata agacagctat gtgggcatta tcaggaaatt ttgatgcatc aaaaagttgg
    23041 aagaaagaag aattagagtt agcgaactgg ataagcaaaa attattccat caatcctgta
    23101 gataatgact ttaggacaac aacacttaca ttaaaagggc atactggtca taaacctcag
    23161 ataaatataa gtggcgataa aaatcattat acttatacag aaaattggga tgagaatacc
    23221 catgtttata ccattacggt taatcataat ggaatggtag agatgtctat aaatactgaa
    23281 gggacaggcc cagtcccttt cccaacacca gataaattta atgatggtaa tttgaatata
    23341 gcatatgcaa aaccaacaac acaaagttct gtagattaca atggagaccc taatagagct
    23401 gtggatggta acagaaatgg taattttaac tctggttcgg taacacacac tagggcagat
    23461 aatccttctt ggtgggaagt cgatttgaaa aaaatggata aagttgggct tgttaaaatt
    23521 tataatcgca cagatgctga gactcaacgt ctatctaatt ttgatgtgat tctatatgac
    23581 aataatagaa acgaagttgc taagaaacat gttaataatt tgtcgggtga atctgttagt
    23641 ctagatttca aagaaaaagg agcaaggtat attaaagtta aattactaac gagtggagtg
    23701 cctttgagtt tagcagaagt agaggttttt agagaatcag atggtaagcg atctgaagag
    23761 gatatagata aaataacaga agataaagta gtctctacaa ataaggtagc tactcaaagt
    23821 tcaaccaatt atgagggtgt agctgcttta gcagttgatg gtaataaaga tggagattac
    23881 ggacatcatt cggtgactca tactaaggca gattctaacg cttggtggca ggtcgatctg
    23941 ggagaagagt ttacggtttc taaagttgat atttataata gaacagatgc cgaacctcag
    24001 cgtttatcta attttgatgt tatttttcta tcttcatcag gagaagaagt ttttagaaga
    24061 cattttgata aagtagttga tggtttgtta tctttaaaag taccttctgt aggggctaag
    24121 ctagtcaaaa tagaattaaa atcagcagct attccgttaa gtttagcgga agttgaagtc
    24181 tatggttcaa agagaactcc gaagaaactt tctaatattg cattaaccaa agaaactcga
    24241 cagagttcaa cggattacaa tggtttttct cgtctagcag ttgatggaaa taaaaacgga
    24301 gattatggtc atcattcagt gactcatacc aaaggagatt ctccttcatg gtgggagata
    24361 gatttagcac aaaccgaaga attagaaaag ttaattattt ataatagaac agatgctgaa
    24421 attcagagat tatcaaattt tgatattatt atatatgatt caaataatca tgaagttttt
    24481 aaacaacata ttgacagttt agaaagcaat aatctatcca tagacttaaa agggctgaag
    24541 ggaaaaaagg ttagaatttc tttgagaaac gcaggaattc ctttaagttt ggcagaggta
    24601 gaggtttata cttataagta attttaaaaa ttatcatcgg tagtatgaaa gaaacaaaaa
    24661 aataagagga aagtattatg atccaacatc cacgtattgg gattcgtccg actattgatg
    24721 gtcgtcgtca aggtgtacgc gaatcacttg aagtacaaac aatgaacatg gctaaaagtg
    24781 tggcagattt gatttcaagc acattgaaat atccagatgg ggaacctgtg gaatgtgtga
    24841 tttctccatc taccattggt cgtgttccag aggctgcagc ttcccatgag ttgtttaaaa
    24901 aatcaaatgt ttgcgcaaca attacagtta caccatgctg gtgttatggt agtgaaacta
    24961 tggatatgtc tccagatatt cctcatgcta tttggggatt taatgggaca gaacgcccag
    25021 gagctgtcta tcttgcagct gtactagctt cacatactca aaaagggatt ccagcctttg
    25081 ggatttatgg tagagatgtt caggaagcta atgatacagc tattccagaa gatgtcaaag
    25141 aaaaactttt acgttatgcg cgggcagctc ttgcaactgg cttgatgaga gacactgctt
    25201 acctatcaat gggtagtgtt tcgatgggga ttggtggttc tattgtaaat ccggatttct
    25261 tccaagaata cctaggaatg cgaaatgaat cggtagatat gacggagttc acgcgccgta
    25321 tggaccgtgg tatttacgac cctgaagagt tcgaacgtgc gctcaaatgg gtgaaagaaa
    25381 acgtaaaaga aggattcgac cataaccgtg aagaccttgt tttaagccgt gaagaaaaag
    25441 atagacaatg ggaatttgtt attaagatgt tcatgattgg acgtgactta atggttggta
    25501 acccaagact tgctgaactt ggttttgagg aagaagcggt tggtcaccat gctttagtag
    25561 ctggtttcca aggtcaacgt cagtggacag accattttcc aaatggggac tttatggaaa
    25621 ctttcctcaa tactcagttt gactggaatg gtattcgaaa accatttgta tttgcgacag
    25681 agaatgattc actaaatggt gtgtctatgc tctttaatta tctattaaca aatactccac
    25741 aaatctttgc tgatgtgcgt acttattgga gcccagaggc tgttaaacgt gtaacgggac
    25801 atactttaga gggtcgtgct gcagctggct tcttacatct aatcaactct ggttcttgta
    25861 cattggatgg tacaggtcaa gctactcgag atggcaaacc tattatgaaa ccattctggg
    25921 agttggaaga aagtgaagtg caggctatgc ttgaaaatac agacttccca ccagcaaacc
    25981 gcgaatactt ccgtggagga ggattctcaa ctcgtttctt gacgaagggg gatatgccag
    26041 taacaatggt acgtctcaat cttctaaaag gggttggtcc agtgctacaa attgcagaag
    26101 gttatacact tgaacttcct gaagatgttc accatacttt agataatcgt acagatccag
    26161 gatggccaac tacttggttt gctccacgtt tgacaggaaa aggtgctttc aagtctgtct
    26221 atgacgtcat gaataattgg ggagctaatc acggagccat aacatatgga cacattggag
    26281 cagacttgat taccttggct tctatgttga gaattcctgt caatatgcat aatgtacccg
    26341 aggaagatat ctttagacct aaaaattggt ccttatttgg aacagaagat ctagaatcag
    26401 cagactatcg tgcatgtcag ttgttgggac cgctacataa ataaaaattg tgtatatagg
    26461 aggtgaactt atgtccctcc tatcctttta aaaagatttg ttaaacaatt cacaaataat
    26521 tgaaaacgaa tacaaaaact aatataatga tgttaaatag atagcgcgga ggcgcaggag
    26581 gaaaattata tggctacatt ttatgttccg gcagtcaacc ttattggtaa aggtgttgta
    26641 aatgaagtgg gtccttatat caaggaactt ggctataaaa aggcactttt ggtgacagat
    26701 aagtacatcg aaggcagtga cattttacct aaggttttaa aaccactgga tacagaagga
    26761 atcgaatatg tcatctttag cgatgtagag ccaaacccta cttgtaaaaa tgtcacagat
    26821 ggggtagctg ctttgcaaga acatggctgt gactttatca tcagtcttgg cgggggttct
    26881 ccacaggatg cagctagttg tatttctatc atggctacaa atggtggaaa accacaggat
    26941 tatgaagggc ttcataagtc tgctaaaaaa ggcttgccag ttgtggctat caatacaacg
    27001 gcaggaactt ctgcagaaat cactattaac tatgtgatta ctgatgaaga acgcaaggtt
    27061 aagatggtaa tggttgacaa gaatagcctt gctcttatct ctgttaatga cccggaactc
    27121 atgctttcca aacctaaagg cctgactgct gctactggta tggatgctct gactcacgct
    27181 gttgaagctt tggtaacacc tggtgcttat gatgtaacca agaaactgtc tattggtgct
    27241 attgagctta tcaaggaata tcttcctcgt gctgtagaaa atggacatga tattgaagcg
    27301 cgtgaaggta tggtcaatgc catcttcctt ggtggtatga gctttaataa tgctggtctt
    27361 ggttatgttc actcaatggc tcaccaactc ggtgcagtat ataatttgcc acatggtgtt
    27421 tgctgtgcca tgttgctacc agttatagaa cgtgaaaatg ctaaacgtgt accagaagct
    27481 ttccgcaatg ttgccaaagc cttgggactt catgtagaag gtaaatcaga tcaagaatgt
    27541 gccgattatg cgattgttga gattgagaag ctttctgaga cagtaggtat tcctaagaaa
    27601 ctgactgaac ttggtattga agaaaaagat ttcgactttg aatacctttc taagaatgcc
    27661 ttgattgatg cctgcgcacc aggaaatcca tttatgccaa ccttagaaga aacgattgcc
    27721 ttttataaag agttatttta gcaagagatt ggacgacaat aggacaattc acctctaatt
    27781 attgacattt caggaaaaat cgcagtaaaa acggcacagc cttctttaaa tgtgctataa
    27841 tactagaaaa atacttgtgg aggttccatt atggcaatat ttttcatgat ttttctgatt
    27901 gtttgtgtgc tcctattggt gatagtcaca ctgagtacag tttatgtggt tcgtcagcag
    27961 tcggtggcga ttattgaacg ctttgggaaa taccaaaagg ttgctaatag cggtattcat
    28021 attcgcttgc cttttgggat tgactcgatt gcagcacgga ttcagctgcg cttgttgcaa
    28081 agtgatattg tggttgagac taagaccaag gacaatgtgt tcgttatgat gaatgtagcg
    28141 actcagtacc gtgtcaacga gcagagcgtg acagatgctt actataaact catacgtcca
    28201 gaatctcaga ttaaatctta tatcgaagat gctcttcgct cttctgttcc aaaattaacc
    28261 ttggatgaat tgtttgagaa aaaagatgag attgcccttg aggttcaaca ccaagtagca
    28321 gaagaaatga ccacttacgg ctacattatc gtgaaaacct tgattaccaa agtcgaaccg
    28381 gatgcagaag ttaagcaatc catgaatgaa atcaatgcgg cgcaacgtaa gcgggtcgca
    28441 gcacaagaat tggcggaagc tgacaagatt aaaattgtca ctgcagctga agccgaagca
    28501 gaaaaagacc gccttcatgg tgtggggatt gcccaacaac gtaaggcgat tgtggatgga
    28561 ttggcagagt ctatcaccga actcaaggaa gccaatgttg gcatgacaga agaacaaatc
    28621 atgtctatcc tcttgactaa ccagtatttg gataccttga atacctttgc ctctaaagga
    28681 aatcaaacca tctttttacc aaatacgcca aatggtgtgg atgatatccg aacacaaatc
    28741 ttgtcagccc ttcgcgctga gaagaaataa tagactaata ctcttcgaaa atctcttcaa
    28801 accacgtcag cgtcgccttg ccgtatagat gttactgact tcgtcagttc tatctgcaac
    28861 ctcaaaacag tgttttgagc tgacttcgtc agtcttatct actaccttaa aacggtgttt
    28921 tgagcaacct gtggctagtt tcctagtttg ctctttgatt ttcattgagt acaaagagct
    28981 tggcaacagg ctctttttgt attgcgttta ttaagaaaaa caaaaaacat cgatatacat
    29041 ttaactaaaa attattgtat gttatctctc ttaattagga atgataaggg aataactaga
    29101 aagatttgtg aatacaaact atttctgata tacaaaatat acagtaataa tgaatgatgg
    29161 gagatgggat gaaagaattt caatttgaga gaaagcagcg tttttctttg aggccatatg
    29221 caataggagc ttgttcggtc ttgctaggaa cgagtttatt ttttgatggt atgggtgatc
    29281 agcctgtaca ggatacagaa acgagttcag cactaatttt aagtcattat ttggatgagc
    29341 aggatttatc tgaaaagctg aaatctgagt tgcaatggct tgaattagaa aacaagcttt
    29401 tgaacttatg ggagcattag gttactatga aggatttgtt ccttatgttt caaattaata
    29461 caaaaaccaa gctgaagaag aagacaaacc gctatctgat aaatatattt tcgaaaaaat
    29521 attaggaaaa acatatgcag ccttcaaaaa agatcaaatt aatgagcgtg tcgagaaatt
    29581 aggtaagtta aaacctatta caataaatta caacggaaaa tcagaagtaa ttgatagtaa
    29641 agaaaaatta caagagctta tgaataaagc cgttaaagac gaagtggctc aaatatagta
    29701 aaatgaaata agaacaggac aaatcaatca ggacagtcaa attgatttct aaaaatgttt
    29761 tagaagtaga ggtgtactat tctagtttca acatactata aaatcaggca atacaacggc
    29821 taaaaagttt gagtttatcg aaataccagt gcaaaaactg aaaaaagcga tttataaagc
    29881 tcatctcaaa gattcagat
//
LOCUS       DAVKQL010000027        26221 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVKQL010000027 DAVKQL010000000
VERSION     DAVKQL010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26221
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.027"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            13..882
                     /gene="rfbA"
                     /locus_tag="VW561_001090"
     CDS             13..882
                     /gene="rfbA"
                     /locus_tag="VW561_001090"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEU9943361.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIRDILIISTPQDLHRFQELLQDGSEFGIKLSYAEQPSPDGLAQAFIIGEEFISDDSV
                     ALILGDNIYHGSGLSKMLQKAASKESGATVFGYHVKDPERFGVVEFDQDMKAISIEEK
                     PEQPRSNYAVTGLYFYDNDVVEIAKSIKPSPRGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEASQYIETVQRMQNVQVANLEEIAYRMGYISREDVLTLAQPLKKN
                     EYGQYLLRLIGEA"
     gene            883..1476
                     /locus_tag="VW561_001091"
     CDS             883..1476
                     /locus_tag="VW561_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEU9943362.1"
                     /translation="MTDNFFGKTLAARKVEAIPGMLEFDIPVHGDNRGWFKENFQKEK
                     MLPLGFPESFFAEGKLQNNVSFSRKNVLRGLHAEPWDKYISVADGGKVLGSWVDLREG
                     ETFGNTYQTVIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPSLGIEWENIAEAEVSEADKHHPLLKDVKPLKKEDL"
     gene            1489..2538
                     /gene="rfbB"
                     /locus_tag="VW561_001092"
     CDS             1489..2538
                     /gene="rfbB"
                     /locus_tag="VW561_001092"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEU9943363.1"
                     /translation="MTEYKNIIVTGGAGFIGSNFVHYVYENFPGVHVTVLDKLTYAGN
                     RANIEEILGNRVELVVGDIADAELVDKLAAQADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQAAD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQEWWKAEKE
                     AVEANYAKTQEIITV"
     gene            2604..3455
                     /gene="rfbD"
                     /locus_tag="VW561_001093"
     CDS             2604..3455
                     /gene="rfbD"
                     /locus_tag="VW561_001093"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEU9943364.1"
                     /translation="MILITGANGQLGTELRYLLDERNEEYVAVDVAKMDITNEEMVEK
                     VFEEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTKNVAKASEKHGATLVYISTD
                     YVFDGKKPVGQEWEVDDRPDPQTEYGRTKRMGEELVEKHVSNFYIIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYLAKNRKEFGYYHLSNDATEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALQE
                     FYKQEVR"
     gene            3518..3838
                     /locus_tag="VW561_001094"
                     /pseudo
     CDS             3518..3838
                     /locus_tag="VW561_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272501.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
     gene            4009..4125
                     /locus_tag="VW561_001095"
     CDS             4009..4125
                     /locus_tag="VW561_001095"
                     /inference="COORDINATES: protein motif:HMM:NF015247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943365.1"
                     /translation="MPVRLTYDNNYFNDTYQGIPIGGYTQIVEKCWIMKILM"
     gene            4101..4685
                     /locus_tag="VW561_001096"
     CDS             4101..4685
                     /locus_tag="VW561_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HEU9943366.1"
                     /translation="MDHENIDVETNVDFFVNKEQYLKDFPKIVFTGMIDEFFDYKLGE
                     LEYRSLRFENETLDMENYQENAVVNYTDAEIPYTRIIEHKHFEFGSQAKTIITKEHSK
                     TWEKGDEPYYPVNNDRNNHLYKSYKKLADEQGNVIFGGRLGHYRYYDMHQVIGAALQC
                     VRNELDSYSMKIKEQTRKLAAGCSKHCFEVADGS"
     gene            4922..6904
                     /locus_tag="VW561_001097"
     CDS             4922..6904
                     /locus_tag="VW561_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9943367.1"
                     /translation="MKSSKLFALAGVTLLAATTLAACSGSGSSAKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNLFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVVIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDETTDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
     gene            7205..12508
                     /locus_tag="VW561_001098"
     CDS             7205..12508
                     /locus_tag="VW561_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU9943368.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKEDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKSPTTSTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYDDERTVVSVKTDNQERVKTEDTPAQKETGPVVDDRKVTYDTI
                     QSKVLKAVIDQAFPRVKEYTLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTEKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLAFNYVKAYAHNTRRDNATVNDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HYPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FASLTAPAQAQEGLANAFDGNLSSLWHTSWGGGDVGKPATMVLKEATEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TNNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLRSLASDQGKTPDYKQEIA
                     RPETPEQILPATGESQSDTALFLASVSLALSALFVVKTKKD"
     gene            complement(12675..14834)
                     /locus_tag="VW561_001099"
     CDS             complement(12675..14834)
                     /locus_tag="VW561_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEU9943369.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLTYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGRNPEDWNIPEGLYRNGEFVFKNGARSTWSSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(14831..15427)
                     /gene="recU"
                     /locus_tag="VW561_001100"
     CDS             complement(14831..15427)
                     /gene="recU"
                     /locus_tag="VW561_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEU9943370.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            15493..16020
                     /locus_tag="VW561_001101"
     CDS             15493..16020
                     /locus_tag="VW561_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEU9943371.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            16090..16419
                     /gene="gpsB"
                     /locus_tag="VW561_001102"
     CDS             16090..16419
                     /gene="gpsB"
                     /locus_tag="VW561_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEU9943372.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            16440..16820
                     /gene="rnpB"
                     /locus_tag="VW561_001103"
     ncRNA           16440..16820
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VW561_001103"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            16905..18062
                     /locus_tag="VW561_001104"
     CDS             16905..18062
                     /locus_tag="VW561_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEU9943373.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKTLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            18075..19469
                     /gene="mapZ"
                     /locus_tag="VW561_001105"
     CDS             18075..19469
                     /gene="mapZ"
                     /locus_tag="VW561_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEU9943374.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVNRSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKSAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            19545..20969
                     /gene="gndA"
                     /locus_tag="VW561_001106"
     CDS             19545..20969
                     /gene="gndA"
                     /locus_tag="VW561_001106"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEU9943375.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVSDVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEDQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            20981..21670
                     /locus_tag="VW561_001107"
     CDS             20981..21670
                     /locus_tag="VW561_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9943376.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVFIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            21769..22791
                     /gene="cbpC"
                     /locus_tag="VW561_001108"
     CDS             21769..22791
                     /gene="cbpC"
                     /locus_tag="VW561_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEU9943377.1"
                     /translation="MKLLKKMMPIALAVFFFGLLATSTVFADTMGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIHGTGYRDDLFDNRPVFEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKKTYYFDNNYAVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDASGKML
                     TDWQKVNGKWYYFGSSGSMATGWKYVRGKWYYLDNKNGDMKTGWQYLGNKWYYLRSSG
                     AMVTGWYQDGSTWYYLDAENGDMKIGWTKVNGKWYYLNSSGAMVTGSQTIDGKVYNFA
                     SSGEWI"
     gene            22810..23808
                     /gene="cbpJ"
                     /locus_tag="VW561_001109"
     CDS             22810..23808
                     /gene="cbpJ"
                     /locus_tag="VW561_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpJ"
                     /protein_id="HEU9943378.1"
                     /translation="MKLLKKTMQAGLTVIFFGLLATNTVFADNSEVWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDSLSGEMVVGWQYIPFPSKGSTIGPYPNGIRLEGFPKS
                     EWYYFDKNGVLQEFVGWKTLEIKTKDSVGRKYGEKREDSEDKEEKRYYTNYYFNQNHS
                     LETGWLYDQSNWYYLAKTEINGENYLGGERRAGWINDDSTWYYLDPTTGIMQTGWQYL
                     GNKWYYLRSSGAMATGWYQEGTTWYYLDQPNGDMKTGWQNLGNKWYYLRSSGAMVTGW
                     VKDDSTWYYLNAGNGDMKTGWFQVNGRWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            complement(23926..25158)
                     /locus_tag="VW561_001110"
     CDS             complement(23926..25158)
                     /locus_tag="VW561_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9943379.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALWIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLLALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLTLITSILCQAYIVIFMTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(25201..26163)
                     /locus_tag="VW561_001111"
     CDS             complement(25201..26163)
                     /locus_tag="VW561_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEU9943380.1"
                     /translation="MTSSTEFGNFVSNLSFQHGIAGLLFPLNKLYPPELDSKILSIIK
                     KAVTIRTTHTYEYQYSLLFGDAGYLWLLLHLFSISKNQYYLQLANVTAKKLIENYDTL
                     EEIDFALGKSGVLLSLIKYYQFTNDNTLKIFIHNSIGEIYHYFLQRDTAKESILDYSF
                     AHGYCGIAYALFAYSKVLEPSMFYNDLHTFHTELKKLLEKVTSNTENLGNLQLSWCKG
                     ISGIILYLCMYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLM
                     KKIQQVILACSERDDHGLLMFQGDSGKADLFDFGIGSMGYIGVY"
BASE COUNT         8220 a         4648 c         5923 g         7430 t
ORIGIN      
        1 aggagtttca ttatgaaagg tattattcta gcaggtggtt cggggacacg tttatatcct
       61 ttgactcgcg ctgcatcaaa acaacttatg ccggtttatg ataaaccgat gatttactac
      121 ccactttcaa cattgatgtt ggctgggatt agggatattt tgattatttc cactccacag
      181 gatttacatc gatttcaaga gcttcttcaa gacggatctg agtttgggat caaactttct
      241 tatgcagagc aaccaagtcc agatggtttg gcacaagcct ttatcattgg ggaagagttt
      301 atttctgatg atagcgttgc gctaatctta ggtgataata tctaccatgg ttctgggctt
      361 tccaagatgc tacaaaaggc agcgagtaag gagtcgggag caactgtttt tggctaccat
      421 gtcaaggatc cagagcgctt tggtgtggtt gagtttgatc aggatatgaa ggctatttct
      481 attgaagaaa agccagagca acctcgttca aactatgcag ttacaggtct ctatttctat
      541 gataatgatg tagtagagat tgccaagagt attaaaccaa gtcctcgtgg tgaactggaa
      601 attacagatg taaacaaagc ttacctagat cgtggtgatt tgtctgttga gcttatggga
      661 cgtggctttg cttggctgga tactggaact catgaaagtt tactagaggc ttcacagtac
      721 atcgaaacag tgcaacggat gcaaaatgtt caggtagcaa acttagaaga aattgcctat
      781 cgcatgggtt atatcagtcg tgaagatgta ttgaccttag cccaaccact taagaaaaat
      841 gaatacggac agtatctgct ccgtttgatt ggagaagcat agatgacaga taattttttc
      901 ggtaagacgc ttgcggcacg caaggttgaa gctattccag gcatgttgga gtttgatatc
      961 cccgttcatg gagataatcg tggctggttt aaagaaaatt tccaaaagga aaaaatgctt
     1021 ccgcttggat ttccagagtc tttctttgca gaaggaaaat tgcaaaacaa tgtatccttc
     1081 tcacgtaaaa atgtccttcg agggctccac gcagagcctt gggataagta catctctgta
     1141 gcagatggag ggaaagttct gggttcttgg gttgatctac gcgagggtga aacctttggg
     1201 aatacctatc agacagtaat tgatgcaagc aagggaatct ttgttcctcg aggcgtagct
     1261 aatggcttcc aagttctatc agatacagtg tcatatagct atctggtcaa tgattactgg
     1321 gctctcgagc tcaaacccaa gtatgccttt gtgaactacg ctgatccaag ccttggtatt
     1381 gaatgggaaa atattgcaga agcagaggtt tcagaagcag ataaacatca tcccctactt
     1441 aaggatgtaa aacctttgaa aaaagaagat ttgtaaaaag gaaagaatat gactgaatac
     1501 aaaaatatta tcgtgacagg tggagctggc tttatcggtt ctaactttgt ccattatgtt
     1561 tacgagaact ttccaggtgt tcacgtgaca gtcctagata agttgactta tgctggaaat
     1621 cgcgcgaata ttgaggaaat tttaggtaat cgtgttgagt tagttgttgg tgacattgct
     1681 gatgcggagt tggtagacaa gttggctgct caagcagatg ctatcgttca ttatgcagcg
     1741 gaaagccaca atgataattc gctcaatgat ccatcgccat ttattcatac taacttcatt
     1801 ggaacctata ctcttttaga agctgctcgt aagtatgata ttcgcttcca ccatgtatcg
     1861 acagatgaag tttatgggga tctcccttta cgcgaagatt tgccaggtca tggagaaggg
     1921 ccgggtgaga aatttacggc tgaaaccaag tacaatccaa gctcgcctta ctcatcaacc
     1981 aaggcagcct cagatttgat tgtcaaagcc tgggtgcgtt cttttggagt caaggcaacg
     2041 atttccaact gttcaaataa ctacggtcct tatcaacata tcgaaaaatt catcccacgt
     2101 cagattacta acatcctaag tggtattaag ccaaaacttt acggtgaagg taaaaacgtt
     2161 cgtgactgga ttcataccaa tgaccattct tcaggagttt ggacaatctt gacaaaaggg
     2221 caaatcggtg aaacctactt gattggggct gatggtgaga agaacaataa ggaagttttg
     2281 gaacttatcc ttaaggaaat gggacaagct gcggatgcct atgatcatgt gactgaccgt
     2341 gcaggacatg accttcgcta tgcgattgat gctagcaagc tccgtgatga gttggggtgg
     2401 aaacctgaat ttaccaactt tgaagctggg ctcaaggcaa caatcaagtg gtatacagat
     2461 aaccaagaat ggtggaaagc agaaaaagaa gctgttgaag ccaattatgc taagactcag
     2521 gagattatta cagtataaaa agcaggaaat agctgctttt tattgctata ttgggaagag
     2581 ttacatatta gaaaggtcta gagatgattt taattacagg ggcaaatggc caattaggaa
     2641 cggaacttcg ctatttattg gatgaacgta atgaagaata cgtggcagta gatgtggcta
     2701 agatggacat taccaatgaa gaaatggttg agaaagtttt tgaagaggtg aaaccgactt
     2761 tagtctacca ttgtgcagcc tacaccgctg ttgatgcagc agaggatgaa ggaaaagagt
     2821 tggacttcgc catcaatgtg acggggacaa aaaatgtcgc aaaagcatct gaaaagcatg
     2881 gtgcaactct agtttatatt tctacggact atgtctttga tggtaagaaa ccagttggac
     2941 aagagtggga agttgatgac cgaccagatc cacagacaga atatggacgc actaagcgta
     3001 tgggggaaga gttagttgag aagcatgtgt ctaatttcta tattatccgt actgcctggg
     3061 tatttggaaa ttatggcaaa aacttcgttt ttaccatgca aaatcttgcg aaaactcata
     3121 agactttaac agttgtaaat gatcagtacg gtcgtccgac ttggactcgt accttggctg
     3181 agttcatgac ctacctagct aaaaatcgta aggaatttgg ttattatcat ttgtcaaatg
     3241 atgcgacaga agatacaaca tggtatgatt ttgcagttga aattttgaaa gatacagatg
     3301 tcgaagtcaa gccagtagat tccagtcaat ttccagccaa agctaaacgt ccgctaaact
     3361 caacgatgag cctggccaaa gccaaagcta ctggatttgt tattccaact tggcaagatg
     3421 cattgcaaga attttacaaa caagaagtga gataagtagt agaatgattt tctagtctaa
     3481 taaaagaggc agagaatgaa ctccaaagga gcttaagatg tacgattatc ttgttgttgg
     3541 tgcagtcttt gcccatgaag cagtcttaaa aggaaaaaaa gtaaaagtta ttgaaaaatg
     3601 aaatcatatc gcgggtaata tctatactcg tgaagaggaa ggaattcaag ttcatcaata
     3661 tggtgctcat atcttccata cttctgataa ggagatctgg gattatgtaa atcagtttgc
     3721 agactttaac cgttatacca attctcctgt tgcaaactat aagggtgaga tttataacct
     3781 tccttttaat atgaatacct tcaataaact ctggggagtt gtgacgccag cagaagcaca
     3841 agctaagatt gatgaacaac gtgctatttt aaatggtaaa actcctgaaa atttggaaga
     3901 acaggcgatt tctcttgtag gtacagacat ctacgaaaaa ttaatcaaag actatacaga
     3961 gaaacagtgg ggcaaaccaa ctactgaact tccagccttt attattgttt gccagtacgc
     4021 ctgacctatg ataacaacta ttttaacgat acctatcaag ggattccaat tggtggatac
     4081 actcaaatag ttgaaaaatg ttggatcatg aaaatattga tgtagaaaca aatgttgatt
     4141 tctttgtgaa taaagagcaa tatctgaaag attttcctaa gattgtcttt actggtatga
     4201 ttgatgaatt ctttgactat aagttgggcg aactagagta ccgtagtctt cgttttgaaa
     4261 atgagacctt ggatatggaa aattaccaag aaaatgcagt tgtgaactat acggatgcag
     4321 aaatcccata tactcgcatt attgaacaca aacattttga gtttgggagt caagcaaaga
     4381 ctatcattac taaagaacat tctaaaacat gggaaaaagg tgatgagcct tattatccag
     4441 ttaataatga tcgtaataat catttgtata aatcgtataa aaaacttgct gatgagcaag
     4501 ggaatgttat ctttggtggc cgcttaggac actatcgtta ttacgatatg caccaagtaa
     4561 ttggagcagc tttgcagtgc gtgagaaatg agttagattc atactcaatg aaaattaaag
     4621 agcaaactag gaagctagcc gcaggttgct caaaacactg ttttgaggtt gcagatggaa
     4681 gctgacgcgg tttgaagaga ttttcgaaga gtataaacaa gtaaaactga ctactagtta
     4741 ttatttagaa atagtattaa aaattccttg actatatgat atagttgagg gatttttaaa
     4801 tgatattcat attttttgca aagatgttgt ttgaaaaata attttcaaaa attctgaaaa
     4861 ttctgttgac aactttctga aaagagttta taatggagag aaagttttaa aggagaaaat
     4921 gatgaaaagt tcaaaactat ttgcccttgc tggcgtgaca ttattggcgg cgactacttt
     4981 agctgcatgc tctggatcag gttcaagcgc taaaggtgag aagacattct catacattta
     5041 tgagacagac cctgataacc tcaactattt gacaactgct aaggctgcga cagcaaatat
     5101 taccagtaac gtggttgatg gtttgctaga aaatgatcgc tacgggaact ttgtgccgtc
     5161 tatggctgag gattggtctg tatccaagga tggattgact tacacttata ctatccgtaa
     5221 ggatgcaaaa tggtatactt ctgaagggga agaatacgcg gcagtcaaag ctcaagactt
     5281 tgtaacagga ctaaaatatg ctgctgataa aaaatcagat gctctttacc ttgttcaaga
     5341 atcaatcaaa gggttggatg cctatgtaaa aggggaaatc aaagatttct cacaagtagg
     5401 aattaaggct ctggatgaac agacagttca gtacactttg aacaaaccag aaagcttctg
     5461 gaattctaag acaaccatgg gtgtgcttgc gccagttaat gaagagtttt tgaattcaaa
     5521 aggagatgat tttgccaaag ctacggatcc aagtagtctc ttgtataacg gtccttattt
     5581 gttgaaatcc attgtgacca aatcttctgt tgaatttgcg aaaaatccga actactggga
     5641 taaggacaat gtgcatattg acaaagttaa attgtcattc tgggatggtc aagataccag
     5701 caaacctgca gaaaacttta aagatggtag ccttacagca gctcgtctct atccaacaag
     5761 tgcaagtttc gcagagcttg agaagagtat gaaggacaat attgtctata ctcaacaaga
     5821 ctctattacg tatctagttg gtacaaatat tgaccgtcag tcctataaat acacatctaa
     5881 gaccagcgac gaacaaaagg catcgactaa aaaggctctc ttaaacaagg atttccgtca
     5941 ggctattgca tttggatttg accgtacagc ctatgcctct cagttgaatg gacaaactgg
     6001 agcaagtaaa atcttgcgta atctctttgt accaccaaca tttgttcaag cagatggtaa
     6061 aaactttggc gatatggtca aagagaaatt ggttacttat ggggatgaat ggaaggatgt
     6121 taatcttgca gattctcagg atggtcttta caatccagaa aaagccaagg ctgaatttgc
     6181 taaagctaaa tcagccttac aagcagaagg tgtgacattc ccaattcatt tggatatgcc
     6241 agttgaccaa acagcaacta caaaagttca gcgcgtccaa tctatgaaac aatccttgga
     6301 agcaacttta ggagcggata atgtagtcat tgatattcaa caactacaaa aagacgaagt
     6361 aaacaatatt acatattttg ctgaaaatgc tgctggcgaa gactgggatt tatcagataa
     6421 tgtcggttgg ggtccagact ttgccgatcc atcaacctac cttgatatca tcaaaccatc
     6481 tgtaggagaa agtactaaaa catatttagg gtttgactca ggggaagata atgtagctgc
     6541 taaaaaagta ggtctatatg actacgaaaa attggttact gaggctggtg atgagactac
     6601 agatgttgct aaacgctatg ataaatacgc tgcagcccaa gcttggttga cagatagtgc
     6661 tttgattatt ccaactacat ctcgtacagg gcgtccaatc ttgtctaaga tggtaccatt
     6721 tacaatacca tttgcattgt caggaaataa aggtacaagt gaaccagtct tgtataaata
     6781 cttggaactt caagacaagg cagtcactgt agatgaatac caaaaagctc aggaaaaatg
     6841 gatgaaagaa aaagaagagt ctaataaaaa ggctcaagaa gatctcgcaa aacatgtgaa
     6901 ataactgttg caaaatataa gaaaggattt agtatttccc ttgaatgctg aatccttttt
     6961 tacatttgta aagaaagatt ctaaatgtac ggacccccaa aagttggagc ctctttttgt
     7021 cagaatagag aaaatttttg ttaattttac ttgtttccta ttgctttctc agctattatt
     7081 tgttatatta aaagtataat tattttttat ttatcagggt taagcattgc actttcagag
     7141 gaaggagtat tttttaaaaa gaaaatgtaa acgcttgctc aaaaatgaaa ggatttagaa
     7201 gtttatgaat aaaggattat ttgaaaaacg ttgtaaatat agtattcgga aattttcatt
     7261 aggtgttgct tctgttatga ttggagctgc attctttggg acaagtccgg ttcttgcaga
     7321 tagcgtgcag tctggttcca cggcgaactt accagctgat ttagctactg ctcttgcaac
     7381 agcaaaagag aatgatgggc gtgattttga agcgcctaag gtgggagaag accaaggttc
     7441 tccagaagtt acagatggac ctaagacaga agaagaacta ttagcacttg aaaaagaaaa
     7501 accggctgaa gaaaaaccaa aagaggataa acctgcagct gctaaacctg aaacgcctaa
     7561 gacggtaacc cctgaatggc aaacggtaga gaaaaaagaa caacagggaa cagtcactat
     7621 ccgagaagaa aaaggtgtcc gctacaacca actatcctca actgctcaaa atgataacgc
     7681 aggtaaacca gctctgtttg aaaagaaggg cttgaccgtt gatgccaatg gaaatgcaac
     7741 tgttgattta accttcaaag aagattctga aaagggcaaa tcacgctttg gtgtcttctt
     7801 gaaatttaaa gataccaata ataatgtttt tgtcggttat gacaaggatg gctggttctg
     7861 ggagtataaa tctccaacaa ctagcacttg gtatagaggt agtcgtgttg ctgctcctga
     7921 aacaggatca acaaaccgtc tctctatcac tctcaagtca gacggtcagc taaatgccag
     7981 caataacgat gtcaatctct ttgacacagt gactctacca gctgcggtca atgaccatct
     8041 taaaaatgag aagaagattc ttctcaaggc gggctcttat gacgatgagc gaacagttgt
     8101 tagcgttaaa acggataacc aagagagggt aaaaacagag gatacccctg cccagaaaga
     8161 aacaggtccg gttgttgacg atagaaaggt gacttatgat acgatccagt ctaaggtact
     8221 taaggcagta attgaccaag ccttccctcg tgtcaaggaa tacaccttga atggacatac
     8281 tttgccagga caggtgcaac aattcaacca agtctttatc aataaccacc gaatcacccc
     8341 tgaagtcact tataaaaaaa tcaatgagac aacagcagag tacttgatga agcttcgcga
     8401 tgatgctcac ttaatcaatg cggaaatgac agtacgcttg caagttgtgg acaatcaatt
     8461 gcactttgat gtgaccaaga ttgtcaacca caatcaagtc actccaggtc aaaagattga
     8521 tgacgaaaga aaactacttt cttctattag tttcctcggc aatgctttag tctctgtttc
     8581 tagtgatcaa actggtgcta agtttgatgg ggcaaccatg tcaaacaata cgcatgtcag
     8641 cggagatgat catatcgatg taaccaatcc aatgaaggat ttggctaagg gttacatgta
     8701 tggatttgtt tctacagata agcttgctgc tggtgtttgg agtaactctc aaaatagcta
     8761 tggtggtggt tcgaatgact ggactcgttt gacagcttat aaagaaacag tcggaaatgc
     8821 caactatgta ggaatccaca gctctgaatg gcaatgggaa aaagcttata agggcattgt
     8881 tttcccagaa tacacgaagg aacttccaag tgctaaggtt gttatcactg aagatgccaa
     8941 tgcagacaag aaagttgatt ggcaagatgg tgccattgct tatcgtagca ttatgaacaa
     9001 ccctcaaggt tgggaaaaag ttaaggatat cacagcttac cgtatcgcga tgaactttgg
     9061 ttctcaagca caaaacccat tccttatgac cttggatggt atcaagaaaa tcaatctcca
     9121 cacagatggt cttgggcaag gtgttctcct taaaggatat ggtagcgaag gccatgactc
     9181 tggtcacttg aactatgctg atattggtaa gcgtatcggt ggtgtcgaag acttcaagac
     9241 cctaattgag aaggctaaga aatatggagc tcatctaggt atccacgtta acgcttcaga
     9301 aacttatcct gagtctaaat acttcaatga aaaaattctc cgtaagaatc cagatggaag
     9361 ctatagctat ggttggaact ggctagatca aggtatcaac attgatgctg cctatgacct
     9421 agctcatggt cgtttggcac gttgggaaga tttgaagaaa aaacttggtg acggtctcga
     9481 ctttatctat gtggacgttt ggggtaatgg tcaatcaggt gataacggtg cctgggctac
     9541 ccacgttctt gccaaagaaa ttaacaaaca aggctggcgc tttgcgatcg agtggggcca
     9601 tggtggtgag tacgactcta ccttccatca ctgggcagct gacttgacct acggtggcta
     9661 caccaataaa ggtatcaaca gtgccatcac ccgctttatc cgtaaccacc aaaaagatgc
     9721 ttgggttggg gactacagaa gttatggtgg tgcagccaac tatccactgc taggtggcta
     9781 cagcatgaaa gactttgaag gctggcaagg acggagcgac tacaatggct acgtgactaa
     9841 cctctttgcc catgatgtca tgaccaagta tttccaacac ttcactgtaa gtaaatggga
     9901 aaatggtaca ccggtgacta tgaccgataa cggtagcacc tataaatgga ctccagaaat
     9961 gcgagtggaa ttggtagatg ctgacaataa taaagtagtt gtaactcgta agtcaaatga
    10021 tgtcaatagc ccacaatatc gcgaacgtac agtaactctc aacggacgtg tcatccaaga
    10081 tggttcagct tacttgactc cttggaactg ggatgcaaat ggtaagaaac tttctactga
    10141 gaaggaaaag atgtactact tcaatacgca ggccggtgca acaacttgga cccttccaag
    10201 cgattgggca aagagcaagg tttaccttta caagctaact gaccaaggta agacagaaga
    10261 gcaagaacta actgtaaaag atggtaaaat taccctagat cttctagcaa atcaaccata
    10321 cgttctctat cgttcaaaac aaaccaatcc tgaaatgtca tggagtgaag gcatgcacat
    10381 ctatgaccaa ggatttaaca gtggtacctt gaaacattgg accatttcag gcgatgcttc
    10441 taaggcagaa attgtcaagt ctcaaggggc aaacgatatg cttcgtattc aaggaaacaa
    10501 agaaaaagtt agtctcactc agaaattaac tggcttgaaa ccaaatacca agtatgccgt
    10561 ttatgtcggt gtcgataacc gtagtaatgc caaggcgagc atcactgtaa atactggtga
    10621 aaaagaagtg actacttata ccaataagtc tctcgcgttc aactatgtta aggcttacgc
    10681 ccacaataca cgtcgtgaca atgctacagt taacgataca agttacttcc aaaacatgta
    10741 cgccttcttt acaacaggat cggacgtctc aaatgttact ctgacattga gtcgtgaagc
    10801 tggtgatcaa gcaacttact ttgatgaaat tcgtaccttt gaaaacaatt caagcatgta
    10861 tggagacaag catgatacag gtaaaggcac cttcaagcaa gactttgaaa atgttgctca
    10921 gggtatcttc ccatttgtag tgggtggtgt tgaaggtgtt gaagataacc gcactcactt
    10981 gtctgaaaaa cactatccat atacacaacg tggttggaat ggtaagaaag tcgatgatgt
    11041 tatcgaagga aattggtcac tcaagacaaa tggactagtg agccgtcgta acttggttta
    11101 ccaaactatt ccgcaaaact tccgttttga agcaggtaag acctaccgtg taacctttga
    11161 atacgaagca ggttcagaca atacctatgc ttttgtagtc ggtaagggag aattccagtc
    11221 aggtcgtcgt ggcactcaag caagcaactt ggaaatgcat gaattgccaa atacttggac
    11281 agattctaag aaagccaaga aggcaacctt cctcgtgaca ggtgcagaaa caggggatac
    11341 ttgggtaggt atctactcaa ctggaaatgc aagtaatact cgtggtgatt ctggtggaaa
    11401 tgccaacttc cgtggttata acgacttcat gatggataat cttcaaatcg aagaaattac
    11461 cctaacaggt aagatgttga cagaaaatgc tctgaagaac tacttgccaa cggttgccat
    11521 gactaactac accaaagagt ctatggatgc tttgaaagag gcggtcttta acctcagtca
    11581 ggccgatgat gatatcagtg tggaagaagc gcgtgcagag attgccaaga ttgaagcctt
    11641 gaagaatgct ttggttcaga agaaaacggc tttggtagca gatgactttg caagtcttac
    11701 agctcctgct caggctcaag aaggtcttgc aaatgccttt gatggaaact tatctagttt
    11761 atggcataca tcatggggcg gaggagatgt aggcaagcct gcaaccatgg tcttgaaaga
    11821 agcaactgaa atcactggac ttcgttatgt tccacgtgga tcaggttcaa atggtaactt
    11881 gcgtgatgtg aaacttgttg tgacagatga gtctggcaag gagcatacct ttactgcaac
    11941 tgattggcca gataacaata agccaaaaga cattgatttt ggtaagacaa ttaaggctaa
    12001 gaaaattgtc cttacaggta ctaagactta cggagatggt ggcgataaat accaatctgc
    12061 agcggaactc atctttactc gtccacaggt agcagaaaca cctcttgact tgtcaggcta
    12121 tgaagcagct ttggctaagg ctcagaaatt aacagacaaa gacaatcaag aggaagtagc
    12181 tagcgttcag gcaagcatga aatatgcgac gaataaccat ctcttgacgg aaagaatggt
    12241 ggaatacttt gcagattatc tcaaccaatt aaaagattct gctacgaaac cagatgctcc
    12301 aactgtagag aaacctgagt ttaaacttag atctttagct tccgatcaag gtaagacgcc
    12361 agattataag caagaaatag ctagaccaga aacacctgaa caaatcttgc cagcaacagg
    12421 tgagagtcaa tctgacacag ccctcttcct agcaagtgtt agcctagccc tatctgctct
    12481 ctttgtagta aaaacgaaga aagactagta tttagtaaaa cctcttaaca agattacgga
    12541 agcagtctct atcttttcca atgaggttta tagtacagaa aaagcctgag aagatatctt
    12601 ctcaggcttt tgttaagcac ataaatacaa tagtgctatg aaaaaatcac ccagaaaaat
    12661 ctggatgata aatgttatgg ttgtgctggt tgaggattct gattttgttg atcaggggtt
    12721 gtatttgatt gttgcgtatt attgttagga ttggtagtcg tactattatt tgtgcttgga
    12781 gtggttgagc tagactgtga agttgaacta tctgatgatg agcttgaact ttcagttgat
    12841 gggggttgtt gtggagcagg tgagctccac gtagaacgag caccattttt aaatacgaat
    12901 tctccatttc tgtagagccc ctctggtata ttccaatcct ctggatttct tccttcagac
    12961 aggtaggtca tcatagagcg gtaaactttg gcagcgaccg taaggccatt gcctacaagt
    13021 ggtgtcagac ggttagaata gcctgtccat acagccattg aatatttacg cgtatagcca
    13081 gcaaatagtt catcaggtgc tacaaattga gaggtcttga tgtggttttc aatttcctcg
    13141 tctgtatagt tagaggttcc tgttttacca gcctgaggga gccaagcaag ataggcattt
    13201 cgtccagttc cataagtcaa gactgttttc atcatgtcgg tcatcatata ggctgtcgtt
    13261 tccttcatgg cacgagttcc gacattagag aactcttttt cactcccatc actaaagacg
    13321 actttatgga tatacattgg tttatagtaa gttccaccat ttgcaaaggc agcgtaagca
    13381 gcagccatct tttcactact tgctccatat tttttgtctg attcggttgt gttacttgaa
    13441 atggcatttg agtagtgaat acttgggtag tcgattccga gaccatttag gaaagtcttg
    13501 gcgcggttga gtccgacctt gtttagagtt tccacggctg ggacgtttcg cgattgttgc
    13561 agggcgtatt gcaaggtgat gttgccaaag tagcccctat cccagttata aacaggagta
    13621 tttgtcccag ggtagttata gggctcatcg tgaacgatag tagcagttga atcgtagaca
    13681 ccgtactcca aggcaggagc atagtctgtg atcggtttca tagttgatcc ccagtcgcgg
    13741 tttgtttcta ctgcttggtt aattccgaag gaaacattgc ttgactgatg gcgtgctcct
    13801 agctgggcaa tgactttacc gttagaaaca tcaacaatgg tagaagcgac ttgcaattca
    13861 tcgtctggat aggcaacgta ttcgtctgta ttgtaaatat cccacagatg tttttgagct
    13921 tcttggtcta catttgtgta gacatccatc ccagttgtga gcaggttata gcctgtttct
    13981 tcttcaactt gattgatgac ttccttgagg taattatcca tgtaagcagg gtaattactt
    14041 gctgatttga gactttgtag tccatcagta attggtgtat tgactgcttt ctcatactgt
    14101 tcagcagaga tgtagccttg atttttcatt tcagataaga ccaagtttcg gcggtcttgg
    14161 gctgcttctg gatgtgaata ggggtcatat tggtttggtg cctgaggcat tccagccagc
    14221 aaggctaact gaggtaaact taaattattg aggtctttac catagtagtt ttgagctgct
    14281 gtctgcattc catagttccc attagacatg tagaccttat ttatatagta ggtcaagatt
    14341 tcttgcttgg ttgctttttg ttctaactga atcgctaacc aagcttcctg agccttacga
    14401 gaaatagtct ggtcggaagt cgaagttgaa aagtaagtca acttaatcaa ctgttgggtg
    14461 agagttgatc caccttggag ggaattgctt tgcagattgc gcaagaaagc tcccaggata
    14521 cggatggtat caatccccct gtggtcgaag aagcgatggt cttcgataga aacgattgcc
    14581 ttaaccaaat ctgtgggaat atcattagct tgggcattga cgcggcgttc agaacccaag
    14641 tcagcaatga gttgattttt attgtcgtag attttactag aagttgttgc aactagttta
    14701 ctctcggata ggctaggagc cttgctaacg tagtagaaaa aaactcctcc gcctaagaca
    14761 atggctgcga taaccaagct taagaagcta atgctcagat acttgattag gcgcagaatc
    14821 gttggtttgt tcatcttgtt ttaccaccta ataaatgttc tttgataaca ttgagataag
    14881 gaatttgagg gaaggcacca gccttgattt catatccata ttctcgaata tattcaagtg
    14941 gcattgattt ttgtccctta tcttgatgat agaagcgaat caaatcgaat gccggcaata
    15001 agtaggtttc ttgctgagaa gaaaagtgaa gaaggacaaa gcagattcct tgttgggcaa
    15061 ggacttgttc catatgctga atctgatgtg gatgaaaatt tttcatcgga atcgcacgtt
    15121 tttgttttgt ttccttgact tcaaagtcga tgtaatatcc attataaacg ccagaatagt
    15181 ccgtcgttga agcttgtcga aaataggctt caacaatctt ggcacgactt cgttgtggat
    15241 agtccacttg tacgatttga ataggagttg gtttcttatg tataacagcc aagccctgag
    15301 acaaatagta gtcgttggta gcgttgatca tcttttcaaa agacattcct cgatttgcga
    15361 aatttttggg ttgagaaaga gatgtttgtc ttttttgtga tgaaacttta tgtggatagt
    15421 tgaccataat tctccttatt ggtacaataa catcacttta ttataccata atttgcacag
    15481 aaagggttaa aaatggctac agctttggtt ttaggctatt ctgcttttga tttgggttta
    15541 ttttctgata aggatcctcg tcttaaattg attaaaaaag cgatacgtaa agatttagag
    15601 gctatggcag cagatggggt gtcttggctt gtgtttacag gaagtttggg ttttgaatat
    15661 tgggttttag aggttgctca ggagatgaaa accgaatacg gtttccagtt ggccaccatt
    15721 tttgcttttg aaacccatgg ggaaaattgg aatgaaggca atcagatgaa actgagtcgc
    15781 tttaagcagg tagactttgt caaatatgct tatcctcgct atgaacacaa ggggcagtta
    15841 agagattacc agcagttttt gctggaaaac acgactagtt cttatctttt ttatgatgaa
    15901 gaaaatgaaa caaaactagc ttatttttac caaaagatga aaaatcaaga ggactatttt
    15961 ataaagagat taacatttga tcaattaaat gaacttgctg aaaatttttc cgaaaattga
    16021 agctttgacc ttgatttttg tttgcctttt tttatataat aatactagca agcgagaatg
    16081 gagagagaca tggcaagtat tattttttca gcgaaagata tttttgaaca agagtttgga
    16141 cgtgaagtcc gtggctataa taaagtagaa gttgacgagt ttttggacga tgtcatcaag
    16201 gactatgaaa cctatgctgc cttggtcaag tcacttcgtc aggaaattgc ggatttgaag
    16261 gaagaattaa ctcgtaaacc gaaaccttca ccagttcaag cagaacccct tgaagcggca
    16321 attacaagtt ctatgacgaa ttttgatatt ttgaaacgcc tgaatagatt ggaaaaagaa
    16381 gtttttggta aacaaatttt agataactca gatttttaag tagttatttg agatgtgcaa
    16441 tttttggata atcgcgtgag gagaattgct tctcatgagg aaagtccatg ctagcacagg
    16501 ctgtgatgcc tgtagtgttt gtgctaggcg aaaccataag cctagggacg aggaatcgtt
    16561 acggcagttg aaatggctaa gtccttggat aggccagagt aggcttgaaa gtgccacagt
    16621 gacggagtct ttctggaaac agagagagtg gaacgcggta aacccctcaa gctagcaacc
    16681 caaattttgg tcggggcatg gagtacgcgg aaacgaacgt agtattctga ctgctatcag
    16741 ctagagctgt tagtggtaga cagatgatta tcgaaggaag tggtcctagt cacttctgga
    16801 acaaaacatg gcttatagaa aattgcatat aggttggggc tgagaaattt tctcaacctc
    16861 attttttaaa gtggacatat agaaaggtct tgcaagactg taacatgaaa aaagaattta
    16921 atttaattgc aactgtggca gcagggcttg aggctgtcgt tggtcgtgaa gtgcgagagt
    16981 tgggctacga ttgtcaggtt gaaaatggac gtgttcgttt tcaaggagac gtgagagcta
    17041 ttatcgaaac caacctttgg cttcgggcag cagatcgtat caaaattatc gtaggaacgt
    17101 tcccagctaa gacttttgaa gagctatttc agggagtttt cgctttggat tgggaaaatt
    17161 atttaccact tggagctcgg ttcccgattt caaaagctaa atgtgttaag tccaaacttc
    17221 acaatgagcc cagtgttcag gctatttcta agaaagctgt tgtcaagaaa ttgcagaaac
    17281 actatgctcg cccagaaggg gttcctctga tggagaatgg cccagagttt aagattgagg
    17341 tctctattct caaagatgtg gcaactgtca tgattgatac gaccgggtct agcctcttta
    17401 aacgtggtta tcgtaccgaa aaaggtggcg ctcctatcaa ggaaaatatg gcagcagcca
    17461 ttttacaact ttctaattgg tatccagaca agactttgat tgatccgacc tgtggttcgg
    17521 ggactttctg tattgaggca gttatgattg ctagaaagat ggcgccaggt cttcgtcgct
    17581 cttttgcatt tgaggaatgg aactggatca gcgatcgctt gattcaagaa gtgcgcacag
    17641 aagcggctaa aaaagtagac cgtgagcttg agctggatat catgggctgt gatattgatg
    17701 ctcgcatggt ggaaattgct aaggccaatg ctcaggcagc tggtgttgca ggagacatta
    17761 cttttaagca gatgcgcgtg caggatttac gttccgataa aatcaatgga gtaatcattt
    17821 ccaatccgcc ttatggtgaa cgtttgtcag atgatgcagg ggtgaccaag ctctatgctg
    17881 agatggggca agtatttgca ccgctgaaaa cttggagcaa atttatcctg actagtgatg
    17941 aagcttttga aagcaagtat ggtagccaag cagataagaa gcgtaagtta tacaacggaa
    18001 ccttgaaagt ggatctatat caatattttg gtcagcgtgt caaacggcaa gaggtaaaat
    18061 agaaagggat actcatgagt aaaaaaagac gaaatcgtca taaaaaagaa ggtcaagaac
    18121 cgcaatttga ttttgatgaa gcaaaagagc taacagttgg tcaagctatt cgtaaaaatg
    18181 aagaagtgga agcaggagtc ttgcctgagg attccatttt ggacaagtat gttaagcaac
    18241 acagagatga aattgaggcg gataagtttg cgactcgtca atacaaaaaa gaggagttcg
    18301 ttgaaactca gagtctggat gatttaattc aagagatgcg tgaggctgta gagaagtcag
    18361 aagcttcttc ggaggaagtt ccatcttctg aagacatctt actacccttg cctctggacg
    18421 atgaggagca aggcttggat cctctattgc tagatgatga aaatccaaca gaaatgactg
    18481 aagaagtgga agaggagcaa aacctttctc gtctggatca agaggactca gaaaagaaaa
    18541 gtaaaaaagg ctttattttg accgttttgg cgcttgtatc agtaattatt tgtgtcagtg
    18601 cttattatgt ctaccgtcaa gtgaatcgct cgaccaagga aattgaaact tctcaatcaa
    18661 ctacagccaa tcaatcagat gtggatgatt ttaatacact ttatgacgcc ttttacacag
    18721 atagcaataa aacggctttg aaaaatagcc agtttgataa actgagtcaa ctcaaaacct
    18781 tgcttgataa gctggaaggt agtcgtgaac atacgcttgc caaatctaaa tatgatagtc
    18841 tagcaacgca aatcaaggct attcaagatg tcaatgcaca atttgaaaaa tcagctattg
    18901 tggatggtgt gttggatacc aatgccaaag ctaaatcgga tgctaaattt acagatatta
    18961 aaactgggaa tacggagctt gataaagtgc tagataaggc tatcagtctt ggtaagagcc
    19021 agcaaacaag tacttctagc tcaagttcaa gtcaaactag cagctcaagc tctagtcaag
    19081 caagctcaaa tacgacaagt gagccaaaac caagtagttc aaatgagact agaagtagtc
    19141 gcagtgaagt caatatgggt ctctcgagtg caggggttgc tgttcaaaga agtgccagtc
    19201 gtgttgccta taatcagtct gctattgatg atagtaataa ctctgcctgg gattttgcgg
    19261 atggtgtctt ggaacaaatt ctagcgactt cacgttcacg tggctatatc actggagacc
    19321 aatatatcct tgaacgtgtc aatatcgtta acggcaatgg ttattacaac ctctataagc
    19381 cagatggaac ctatctcttt acccttaact gtaagacagg ctactttgtc ggaaatggcg
    19441 ctggtcatgc ggatgacttg gactactaag cagtcgttac aaaattcttt cttttcaaaa
    19501 gtaaaaatga taaaataaaa caaatgaaac aagaggagtg tcaaatgaca aaagctaact
    19561 ttggtgttgt aggtatggcc gtaatgggtc gtaaccttgc ccttaatatt gaatctcgtg
    19621 gttacacagt tgctatctac aaccgtagta aagaaaaaac ggaagatgtg attgcttgcc
    19681 atcctgaaaa gaactttgta ccaagctatg acgttgaaag ttttgtaaac tcaatcgaaa
    19741 aacctcgtcg tatcatgctg atggttcaag ctggacctgg tacagatgct actatccaag
    19801 cccttcttcc acaccttgac aagggtgata tcttgattga cggaggaaac actttctaca
    19861 aagatactat ccgtcgtaat gaagaattgg caaactcagg tatcaacttt atcggtactg
    19921 gagtttctgg tggtgaaaaa ggtgcccttg aaggtccttc tatcatgcct ggtggacaaa
    19981 aagaggccta cgaattggtt tcggatgttc ttgaagaaat ctcagctaaa gcaccagaag
    20041 atggcaaacc atgtgtgact tatatcggtc ctgatggagc tggtcactat gtgaaaatgg
    20101 ttcacaacgg tatcgagtac ggtgatatgc aattgatcgc agaaagctat gacttgatgc
    20161 aacacttgct aggcctttct gcagaggata tggctgaaat ctttactgag tggaacaagg
    20221 gtgaattgga tagctacttg atcgaaatca cagctgatat cttgagccgt aaagacgatg
    20281 aagaccaaga tggaccaatc gtagactaca tccttgatgc tgcaggtaac aagggaactg
    20341 gtaaatggac gagccaatca tctcttgacc ttggtgtacc attgtcactg attactgagt
    20401 cagtgtttgc acgctacatt tcaacttaca aagaagaacg tgtacatgct agcaaggtgc
    20461 ttccaaaacc agctgccttc aactttgaag gagacaaggc tgaattgatt gaaaaaatcc
    20521 gtcaagccct ttacttctca aaaatcattt catacgcaca aggatttgct caattgcgtg
    20581 tagcctctaa agaaaacaac tggaacttgc catttgcaga tatcgcatct atctggcgtg
    20641 atggctgtat catccgttct cgtttcttgc aaaagattac agatgcttac aaccgcgatg
    20701 cagatcttgc caaccttctt ttggacgagt acttcttgga tgttactgct aagtatcaac
    20761 aagcagtgcg tgatatcgta gctcttgcgg ttcaagctgg tgtgccagtg ccaactttct
    20821 cagcagctat tacttacttt gatagctacc gttcagctga ccttccggct aacttgatcc
    20881 aagcacaacg tgactacttt ggtgctcaca cttaccaacg taaagacaaa gaaggaacct
    20941 tccactactc ttggtatgac gaaaaataag taggtcagcc atggggaaac ggattttatt
    21001 acttgagaaa gaacgaaatc tagctcattt tttaagtttg gaactccaga aagagcagta
    21061 tcgggttgat ctggtagagg aggggcaaaa agccctttcc atggctcttc agacagacta
    21121 tgatttgatt ttattgaacg ttaatctggg agatatgatg gctcaggatt ttgcagaaaa
    21181 attgagtcga actaaacctg cctcagtcat catgatttta gatcattggg aagacttgca
    21241 agaagagctg gaagttgttc agcgttttgc agtttcatac atctataagc cagtctttat
    21301 cgaaaatctg gtagcgcgta tttcggcgat cttccgaggt cgggacttca ttgatcaaca
    21361 ctgcagtctg atgaaagttc caaggaccta ccgcaatctt aggatagatg ttgaacatca
    21421 cacggtttat cgtggtgaag agatgattgc tctgacccgc cgtgagtatg accttttggc
    21481 gacacttatg ggaagtaaga aagtattgac tcgtgagcaa ttgttggaaa gtgtttggaa
    21541 gtatgaaagt gcgaccgaga caaatatcgt agatgtctat atccgctatc tacggagcaa
    21601 gcttgatgtt aaaggccaaa aaagctacat taaaaccgtg cgtggtgttg gttacaccat
    21661 gcaagaatag aaaagcagtt gcagttgtgt aactgctttt ttgaggagtt tttatatatt
    21721 gacatacaat ctagttttta ctacaatcgt tttaggagga tatataaaat gaagcttttg
    21781 aaaaaaatga tgccaatcgc attagcagtc tttttctttg gtttgctagc gacaagtaca
    21841 gtatttgcag ataccatggg tggccgattt gttgataagg ataatagaaa atattatgta
    21901 aaagatgatc ataaagcaat ctattggcat aaaatagacg gtaaaactta ctattttggt
    21961 gatattggag agatggttgt cggttggcaa tacttagaaa ttcatggaac aggttatcgt
    22021 gatgatttat ttgataatcg tccagtcttc gaaattggcc ttcaggagaa gtggtactat
    22081 tttggacaag atggtgcttt gctagaacaa acagataaac aagtactaga ggcaaaaacg
    22141 tctgaaaata caggaaaagt atacggtgaa caatatcctc tatctgctga aaagaaaact
    22201 tattattttg ataataatta tgctgtaaag acaggctgga tttatgaaga cggcaattgg
    22261 tattatttaa ataagctagg aaattttggc gatgattctt acaatccact accaattggt
    22321 gaagttgcta agggttggac tcaagatttt catgttacta ttgacattga tagaagcaaa
    22381 cctgctccat ggtactacct agatgcttca ggtaagatgc ttacagattg gcaaaaagta
    22441 aacggaaaat ggtattattt tggctcctct ggttctatgg caacaggttg gaaatatgta
    22501 cgaggcaaat ggtattactt agataataaa aatggtgata tgaaaacagg atggcaatac
    22561 cttggtaaca agtggtacta cctccgttca tcaggagcta tggtaactgg ctggtatcaa
    22621 gatggttcaa cttggtacta tttagatgct gaaaatggtg atatgaaaat aggttggaca
    22681 aaagtaaatg gaaaatggta ttatctcaat tcctctggag caatggttac aggtagtcaa
    22741 actatcgatg gtaaagttta taacttcgct tcatctggtg agtggattta atgttggagg
    22801 atatataaaa tgaagctttt gaaaaaaact atgcaagctg gactaacagt aattttcttt
    22861 ggtttgctag ctacgaatac agtatttgca gataactctg aagtatggca gtttgtccaa
    22921 gaaaacggta gaacctatta caaaaagggg gacctcaaag aaacctactg gcgagtgatt
    22981 gatggtaagt actattattt tgattctcta tctggagaga tggttgtcgg ctggcaatat
    23041 atcccgtttc catctaaagg tagtacaatt ggtccttacc caaatggtat cagattagaa
    23101 ggttttccaa agtcagagtg gtactacttc gataaaaatg gagtgctaca agagtttgtt
    23161 ggttggaaaa cattagagat taaaactaaa gacagtgttg gaagaaagta cggggaaaaa
    23221 cgtgaagatt cagaagataa agaagagaaa cgttattata cgaactatta ctttaatcaa
    23281 aatcattctt tagagacagg ttggctttat gatcagtcta actggtatta tctagctaag
    23341 acggaaatta atggagaaaa ctaccttggt ggtgaaagac gtgcggggtg gataaacgat
    23401 gattcgactt ggtactattt agatccaaca actggtatta tgcaaacagg ttggcaatat
    23461 ctaggtaata agtggtacta cctccgttcc tcaggagcaa tggccactgg ctggtatcag
    23521 gaaggtacca cttggtatta tttagaccag ccaaatggcg atatgaaaac aggatggcaa
    23581 aaccttggga acaaatggta ctatctccgt tcgtcaggcg ccatggtgac tggctgggtg
    23641 aaagatgact caacttggta ttacctaaat gcaggtaatg gagacatgaa gacaggttgg
    23701 ttccaggtca atggcagatg gtactacgct tatagctcag gtgccttggc agtgaatacg
    23761 accgtagatg gctattctgt caactataat ggcgaatggg ttcggtaatg acagggcgct
    23821 tataattata ttagaacact cgcttaatca aagaagtgac tttgttgtac ttgacctgac
    23881 gattggggag attaaaagca tgtgaaatct acacatgctt tttcgttagt tatgagtgtt
    23941 gaacttgact aaccattgac aaaagataag agcaattaaa ataagataca aaaatatcaa
    24001 tgctaactgc accgaaacgc ccgatgctat ggatagggct aatagggaga gcagggacgg
    24061 ggcgagaatg tccactgtgc tcagagcaga gcccaccgtc cccatcttgt cactggggat
    24121 atgggcaaag acagctgctt gtaggcgagg gctgagaatg ccgataatcg tagaactgat
    24181 gaaactggtc atgaaaatta cgatataggc ttgacaaagg atactagtta ttagagtcaa
    24241 tagggaaatc tcacagaaaa ccaagaggtg cgtgaaacgg atatttttaa atagattact
    24301 gctggtcata ttgccaagga tagctccaca agacatgatg atggagagca gggcgaggag
    24361 ttgcccaatg ctcagttgtg ctagtgtctg ttggatgaag cggagggcaa tcagccgagg
    24421 ggacacatcc aagatggcaa tcatactggt cgtcagaaac agtagtatga cggtatcttt
    24481 taacctcagg agcacagcca gcgactgttt aagatgttga aaatatttct taaaactcag
    24541 tgctgtatgt gacatttcaa ttcttttttc aacctcatac aaggtgtgtc gaataacata
    24601 caggcccaaa aaggcaatga caaaagtcag agtgttgata agggaaatag tttgaatact
    24661 tataacattg ataaggaatg cgccagccag attggcgaca atacggacaa ccctcatcag
    24721 agactgcctg aaccccatgg catcatgcag gtcgtcctta attacactga tgtagagggc
    24781 gttcatcatg taggcagaca ggtagctcaa ggtgtcagag atgaggttga tgatgacaat
    24841 cataatgact gaaactaatg aaaagggcga caagacgaga aatactgtca aaatagcgta
    24901 gagcaggatt ttgattttgg caatccagag tgcgtgtttg actctatttt cttgaaaatc
    24961 tcctagaatc ccgagaaaga gttgcgatag taggggaacc atctctgaaa tggaaatcag
    25021 taaaatcgct agcggagcga aattgtactg agccacataa ttgataatgg cgaggtagaa
    25081 aatactgtcg ccgaatgatg aaaaacagct gtttagcagc aactgtctat atatgttgtt
    25141 tgtccaaaat aatttcatac ttttctcctt atgtctgcac atcgaatggg aatttattat
    25201 ttaatagaca ccaatatacc ccatgcttcc tattccgaag tcaaacaaat ctgctttacc
    25261 actatctcct tgaaacatca gtaaaccgtg atcatctcgt tcagaacatg ctaaaattac
    25321 ctgttggatt tttttcatca gtaatttgtt ttgattgtag acagtggttt gtagtaagct
    25381 agttattccg tggcaatatc ctgtcatcat ttttagatga tggttaaaaa caaattcttg
    25441 atatttacta ataatatctt tgtttccgtc acaatcgtac atacaaagat ataagattat
    25501 tccggaaatt cctttgcacc aagaaagttg taaatttcct aaattttcag tattagaagt
    25561 aactttttct aataattttt ttaattcagt atggaatgta tggagatcat tatagaacat
    25621 agaaggttct aagactttag aataagcaaa taaagcatat gcaattccac aatatccatg
    25681 agcaaagcta tagtctaaaa tgctttcttt ggctgtatct ctttgtagga aataatgata
    25741 aatttcccct atactattgt ggatgaaaat tttaagagta ttgtcattgg taaattgata
    25801 gtattttatt aatgataata ggacaccaga ttttcccaat gcaaagtcta tttcctctag
    25861 agtatcataa ttctctatta attttttagc ggtgacgttt gctaattgta gatagtattg
    25921 atttttactg atagaaaata aatgtaggag taaccataga tagcctgcat caccaaatag
    25981 cagtgagtat tgatattcat atgtatgtgt cgttctaatt gtcactgcct tcttgatgat
    26041 agagagtatt ttagaatcca gttctggggg gtacaattta tttagaggaa acagtagtcc
    26101 agctattccg tgttggaatg ataaattact tacgaaattt ccaaattctg ttgaagaagt
    26161 gatccttcct tcagaattta cagttttatc tagtaaaaat tgtagaacac tattacattc
    26221 t
//
LOCUS       DAVKQL010000028        25360 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVKQL010000028 DAVKQL010000000
VERSION     DAVKQL010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25360
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.028"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(207..404)
                     /gene="blpI"
                     /locus_tag="VW561_001112"
     CDS             complement(207..404)
                     /gene="blpI"
                     /locus_tag="VW561_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HEU9943381.1"
                     /translation="MNTKMMEQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIMSSL"
     gene            686..1273
                     /locus_tag="VW561_001113"
     CDS             686..1273
                     /locus_tag="VW561_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEU9943382.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            1200..1871
                     /locus_tag="VW561_001114"
     CDS             1200..1871
                     /locus_tag="VW561_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin ABC transporter"
                     /protein_id="HEU9943383.1"
                     /translation="MVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVI
                     TYILQQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDA
                     NSIIDALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLISIPIYMFIIFSFMKPFE
                     KMNHDVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYWKNPLSSVNIL
                     FYKRV"
     gene            1820..2842
                     /locus_tag="VW561_001115"
     CDS             1820..2842
                     /locus_tag="VW561_001115"
                     /inference="COORDINATES: protein motif:HMM:NF012869.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9943384.1"
                     /translation="MEKSFKLSKYSILQTSLKQGTKLVLNILILWFGAQLVMSSKISI
                     GQLITFNTLFSYFTTPMENIINLQTKLQSAKVANNRLNEVYLVESEFQVQENPVHSHF
                     LMGDIEFDDLSYKYGFGRDTLTDINLTIKQGDKVSLVGVSGSGKTTLAKMIVNFFEPY
                     KGHISINHQDIKNIDKKVLRRHINYLPQQAYIFNGSILENLTLGGNHMISQEDILKAC
                     ELAEIRQDIERMPMGYQTQLSDGAGLSGGQKQRIALARALLTKAPVLILDEATSGLDV
                     LTEKKVIDNLISLTDKTILFVAHRLSIAERTNRVIVLDQGKIIEVGSHQELMQAQGFY
                     HHLFNK"
     gene            2853..4214
                     /locus_tag="VW561_001116"
     CDS             2853..4214
                     /locus_tag="VW561_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEU9943385.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMDESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLANSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTSPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            4271..4399
                     /gene="blpC"
                     /locus_tag="VW561_001117"
     CDS             4271..4399
                     /gene="blpC"
                     /locus_tag="VW561_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEU9943386.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDLLYNINRYAHYIT"
     gene            complement(4470..5810)
                     /locus_tag="VW561_001118"
     CDS             complement(4470..5810)
                     /locus_tag="VW561_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9943387.1"
                     /translation="MNIAWILLYTLVTNGLEIVIFFKVDGIDLTFERIFKAFLFKILL
                     AFVFVMISYIVGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRKEILDKAFQK
                     SLTQINWIMGGYYLVMESLSFFEYEQSIQSKTVRHLILVFYLLFFMGVIKKLDTYLKE
                     KLYERLEQEQALRYRDMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYGSVLKDSSQKLQNNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASVEASQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQNQVFRQVLTVIHTE"
     gene            complement(5824..6561)
                     /locus_tag="VW561_001119"
     CDS             complement(5824..6561)
                     /locus_tag="VW561_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9943388.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHKKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRAHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINKLH"
     gene            complement(6566..6904)
                     /locus_tag="VW561_001120"
     CDS             complement(6566..6904)
                     /locus_tag="VW561_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9943389.1"
                     /translation="MKYMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLEEGE"
     gene            7180..7509
                     /locus_tag="VW561_001121"
     CDS             7180..7509
                     /locus_tag="VW561_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEU9943390.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(7677..8726)
                     /locus_tag="VW561_001122"
     CDS             complement(7677..8726)
                     /locus_tag="VW561_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9943391.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKVVQKVSGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSLLAQVFIEQSWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLISFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(8723..9454)
                     /locus_tag="VW561_001123"
     CDS             complement(8723..9454)
                     /locus_tag="VW561_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9943392.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            9522..9932
                     /locus_tag="VW561_001124"
     CDS             9522..9932
                     /locus_tag="VW561_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU9943393.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            9942..10229
                     /locus_tag="VW561_001125"
     CDS             9942..10229
                     /locus_tag="VW561_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943394.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIINQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            10391..10630
                     /locus_tag="VW561_001126"
     CDS             10391..10630
                     /locus_tag="VW561_001126"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943395.1"
                     /translation="MHHNLGNSYSIKIKKQTRKLGTDCSKHRFKVVDRTDEVSSKHCF
                     EVVDRTDEVSNHTHDKPTLTWFEEIFEEYHSPFHN"
     gene            complement(10952..12079)
                     /gene="dnaJ"
                     /locus_tag="VW561_001127"
     CDS             complement(10952..12079)
                     /gene="dnaJ"
                     /locus_tag="VW561_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU9943396.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFNGAGGFGGFEDIFSSFFGG
                     GGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGTSPV
                     TCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAHSVH
                     VKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLNLNF
                     VQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVNVVT
                     PTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(12481..12837)
                     /locus_tag="VW561_001128"
     CDS             complement(12481..12837)
                     /locus_tag="VW561_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
                     /protein_id="HEU9943397.1"
                     /translation="MSVLDEEYLKNTRKVYNDFCNQADNYRTSKDFIDNIPIEYLARY
                     REIILAEHDSCIKNDEAVRNFVTSVLLSAFVSAMVPAMISLEIQTYKFVIPFIIGMIW
                     TVVVFLMINWNYIGKY"
     gene            complement(12839..14662)
                     /gene="dnaK"
                     /locus_tag="VW561_001129"
     CDS             complement(12839..14662)
                     /gene="dnaK"
                     /locus_tag="VW561_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEU9943398.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(15142..15666)
                     /gene="grpE"
                     /locus_tag="VW561_001130"
     CDS             complement(15142..15666)
                     /gene="grpE"
                     /locus_tag="VW561_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU9943399.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(15693..16727)
                     /gene="hrcA"
                     /locus_tag="VW561_001131"
     CDS             complement(15693..16727)
                     /gene="hrcA"
                     /locus_tag="VW561_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEU9943400.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFHRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVHLLNFANLEAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(16891..17400)
                     /locus_tag="VW561_001132"
     CDS             complement(16891..17400)
                     /locus_tag="VW561_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEU9943401.1"
                     /translation="MNSRVEFRIFTIVDLDKEEEHLHEMHLKGWRYRTSRFGLFYFDQ
                     CQPDDVIYRIYDSRFLKKYKHELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTTTFLGGLVVCTSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            18007..20340
                     /locus_tag="VW561_001133"
     CDS             18007..20340
                     /locus_tag="VW561_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU9943402.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFKPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKIIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIED
                     RYYSRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGEPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            20353..21816
                     /locus_tag="VW561_001134"
     CDS             20353..21816
                     /locus_tag="VW561_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU9943403.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSNM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLDPKLGESMVDLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGAELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            21816..23384
                     /locus_tag="VW561_001135"
     CDS             21816..23384
                     /locus_tag="VW561_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9943404.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRFSTLVEIVRG
                     GSPRPIKDYLTSEVDGINWIKIGDTEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLT
                     NSMSFGRPYILNVDGAIHDGWLAISNYENSLNKDYLFYILSSNVVYSQFLSLISGAVV
                     KNLNSDKVASILIPLPPLSEQQRIIEAIESALEKVDEYAESYNRLEQLDKEFPDKLKK
                     SILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNS
                     YYEEVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCNQWSGFSIDLAR
                     FIDPETVHSYQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNPYGWAVADSHVTVIRV
                     LSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKEYLIPLPPLPEQSRIV
                     DKIEQFFAHIDALI"
     gene            23441..24238
                     /gene="psrA"
                     /locus_tag="VW561_001136"
     CDS             23441..24238
                     /gene="psrA"
                     /locus_tag="VW561_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HEU9943405.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
     gene            24309..24854
                     /locus_tag="VW561_001137"
     CDS             24309..24854
                     /locus_tag="VW561_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9943406.1"
                     /translation="MNWVVIKIKDIFSINTGLSYKKGDLSINNKGVRIIRGGNIKPLE
                     FSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHIGKFARIDKDYDGVVAGGFIF
                     QLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQALYNIPKTTLSELLIPLAPFE
                     EQELITQKVEKLFEKVNQLWK"
     gene            complement(24802..>25360)
                     /locus_tag="VW561_001138"
     CDS             complement(24802..>25360)
                     /locus_tag="VW561_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9943407.1"
                     /translation="NKDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGT
                     EIPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIP
                     ATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPI
                     SNHEEMKRIISKVDLLFQKVSQLFE"
BASE COUNT         8180 a         4933 c         4623 g         7624 t
ORIGIN      
        1 ataatagttt atagttaagt tttttattcc taccaatcca tcaactaagt aaagcatcaa
       61 cgattacata aacgattgat aatataatta aaattttgct aactatctta ttctcatgat
      121 tcttagataa ctttgatatt ttgtaagtaa gtaaataaga cagtaaatta atagcgataa
      181 taatactata tttaagaatc ataatcttac aaagaggaca taattcctga acctacacaa
      241 ataagtgttg ctgctccccc agttatcgga ccagtcgcag cagctaatag tactgctcca
      301 atacaaccac cgattgcaga tcctaaattg cctcttcctc cactaactat ttcgagttct
      361 tcattatcca taacagaaaa ttgttccatc atttttgtat tcatgacaaa tactcctttt
      421 ttcttttttt atttttgtct tgttgtaact ttgataagtt tagtatatca tcgtttttta
      481 aaatttttca tccagatctt gaattgtcat cgaaacgtct tgaattaggt tttttatttc
      541 aagccacctc taaatgttta aaaaaaataa tttctaatca cttttttacc attcaggaag
      601 ttttaatgac tattcaagat ttcataaaat atgaacttag ttttatgaca taatagacct
      661 atccactata tgaaaggaat tgccaatgac ttcttataaa cgtacatttg ttcctcaaat
      721 agatgcgaga gactgtggtg tcgctgcctt agcctcgatt gctaaattct atggttcaga
      781 tttttctcta gctcacttga gagaacttgc aaagaccaat aaagaaggga cgactgctct
      841 tggcattgta aaagccgctg atgaaatggg ctttgaaaca agacctgttc aagcagataa
      901 aacgctcttt gacatgagtg atgtccccta tccatttatc gttcacgtta acaaagaagg
      961 aaaactccaa cattactatg ttgtctatca aacaaagaaa gactatctga ttattggtga
     1021 tcctgaccct tctgtaaaaa tcactaaaat gtcaaaagaa cgctttttct atgaatggac
     1081 tggagtagct atttttctag ctaccaaacc cagctatcaa ccccataaag ataaaaagaa
     1141 tggtctacta agcaagcttc cttcctctga ttttcaaaca aaaatctctc attgcttaca
     1201 ttgttctctc aagcttattg gtcactatta tcaatatagg tggttcttac tatctccaag
     1261 gaatcttgga tgaatacatt ccaaatcaga tgaaatcaac tttaggaatc atctcagttg
     1321 gtctggttat cacctatatc ctccaacaag tcatgagctt ctccagagat tatctcctaa
     1381 ccgttctgag tcagagatta agtattgatg tgattttatc ctatattcgc catatttttg
     1441 aacttcccat gtctttcttt gcgacacgtc gtacaggaga aatcatttca cgattcacag
     1501 atgctaactc tattatagat gccttggctt ctaccattct ttctcttttt ctggatgttt
     1561 ctattctgat tcttgtagga ggcgtcttac tggcacaaaa ccctaatctc ttccttcttt
     1621 ctcttatttc cattcctata tacatgttca tcatcttttc ttttatgaaa cctttcgaaa
     1681 aaatgaacca tgatgtcatg caaagtaatt ctatggttag ctctgccatt atcgaagata
     1741 tcaacgggat tgaaactata aagtcgctca cgagtgaaga aaatcgctat caaaatatag
     1801 acagcgaatt tgtagattat tggaaaaatc ctttaagctc agtaaatatt ctattttaca
     1861 aacgagttta aagcagggaa caaaattagt tctgaatatc cttatcctat ggtttggcgc
     1921 tcaattagtc atgtcaagta aaatttctat cggtcagctg attaccttta acacactttt
     1981 ttcttacttt acaactccta tggaaaatat tatcaacctc caaaccaaac tccaatctgc
     2041 gaaggtcgct aataaccgtt tgaacgaagt ctatctagtc gaatctgaat ttcaagttca
     2101 agaaaaccct gttcattcac attttttgat gggcgatatt gaatttgatg acctttctta
     2161 taagtatggt tttggacgag ataccttaac agatattaat ctcacgatta aacaaggaga
     2221 taaggttagc ctagttggag ttagtggttc tggtaaaaca actttagcca aaatgattgt
     2281 caatttcttt gaaccctaca aagggcatat ttccatcaat catcaggata ttaaaaacat
     2341 tgataaaaaa gtcttgcgcc gtcatattaa ttacctaccc caacaagcct atatctttaa
     2401 tggctctatt ttggaaaact taaccttggg cggtaatcat atgattagcc aagaagatat
     2461 tctaaaagct tgtgaattag ctgaaattcg tcaagacatt gaaagaatgc ctatgggcta
     2521 tcaaactcag ctctctgatg gagctggtct atcaggagga caaaagcaac gaatcgctct
     2581 cgctcgtgct cttttaacta aagctcctgt tttaatacta gatgaagcta ctagcggtct
     2641 tgatgtcttg actgagaaaa aggttataga taatcttata tccctaactg ataaaaccat
     2701 tctctttgta gcccatcgtc tcagtatagc cgaacgaact aaccgtgtca ttgttcttga
     2761 ccaggggaaa atcattgaag ttggtagtca ccaagagtta atgcaagcgc aaggattcta
     2821 ccatcatcta ttcaataaat aaggagaatg tcatgaatcc taatcttttt agaagcgtcg
     2881 agttttatca gagacgttac cataactatg cgacagtatt aatcatacct ctgtcattac
     2941 tgtttacttt catcttgatt ttctcccttg ttgccacaaa agaaattact gttacttccc
     3001 aaggagaaat cgcccctaca agtgtcattg catctattca gtcaaccagt gataatccta
     3061 tccttgctaa tcatttagtg gcaaatcaag tagttgaaaa aggtgactta ctcatcaaat
     3121 actctgaaac aatggatgag agtcagaaaa ctgccttagc aactcaatta caaagacttg
     3181 agaagcaaaa agaaggactt ggaattttga aacaaagttt agaaaaagcg actgatcttt
     3241 tttctggcga ggatgagttt ggctaccata atacctttat gaattttact aaacaatccc
     3301 atgatattga actgggtatc acaaagacta acaccgaagt ttcaaatcaa gctaatcttg
     3361 ccaatagcag ttcatcagcc atcgaacaag aaattacaaa agttcaacaa caaattggag
     3421 aatatcaaga gttgagagat gctatcataa ataacagagc acgcttacca actggcaatc
     3481 cgcaccagtc aattttgaat cgttatcttg tagcctcaca aggacaaaca caaggaactg
     3541 cagaggagcc atttttatct caaattaatc aaagtattgc aggtcttgaa tcatctatcg
     3601 caagcctcaa aattcagcaa gctggtatcg gaagtgtagc aacttatgat aacagtttag
     3661 caaccaaaat tgaagtactc cgcactcagt ttttacagac agcctcacag caacaactaa
     3721 ctgtggagaa tcaattaaca gaattaaaag tacaactaga tcaagccaca cagcgtttgg
     3781 aaaacaatac cttaacctcc ccaagtaaag gtatcgttca tctgaacagc gaatttgaag
     3841 gtaaaaatag aattccaact ggtacagaaa ttgctcaaat attccctgtc atcacagata
     3901 caagagaagt actaatcact tactacgtat cttctgacta tctacctcta ctagataaag
     3961 gacaaactgt aagattaaaa ctggagaaga ttggaaatca cggcaccacc atcatcggcc
     4021 aacttcagac aattgatcaa actcctacca gaacagagca aggaaatctc tttaaattaa
     4081 ccgctcttgc aaaactatct aacgaggata gtaaactcat ccaatatggc ttacaaggtc
     4141 gcgtcactag tgtaactaca aagaaaacat attttgatta tttcaaagat aaaattttaa
     4201 cccattctga ttaattttca gataacactc tataactatt tattatctta tcaaaaagga
     4261 gaatcataac atggataaga aacaaaacct aacttcattt caagaactaa caactaccga
     4321 actcaaccaa attacaggtg gaggattgtg ggaagattta ttatataaca ttaatagata
     4381 tgctcattac atcacataag aacttcatca tccaatacaa ctataaaaaa ataagaccga
     4441 gaaacaagta ctctcggtct tatttttcat cattctgtat gtatcacagt aagtacctga
     4501 cgaaagactt gattttgaca ggtggtattt agactggtat taggatggct ttccacaatc
     4561 ttcatgacgg tatagagacc aactcctctc tcctcccctt tagaactggc tccaaaggag
     4621 aagatttcag aaatatcgat gccctcttct ttgatggagt tttcgatgat aaaggtctcc
     4681 tgtgctccat tttttaaaaa ggcgattgaa acatgaggtt gactagcttc cacactggct
     4741 tcaatagcat tgtcacaaag gatagacaca atggttagaa aatcaagtag actcatcccc
     4801 tcgacctgaa tctcctcagg aacttcgaca ttaaagacaa tgttcttatc tctggctttt
     4861 aaaaatttcc ctgctagaag acttttgagg gctttatcac gaatatttac caatctgccc
     4921 aggtcatatt tattgttctg caatttctga ctggaatcct ttaagacgga gccatagacc
     4981 tcttttatct gctccatatc ctcctcttca atgcccagac gtaagctagt caagaggttg
     5041 gtataatcat gacgaaagct ccgtacttcc ttgtaaagct cctctatatg ccgactatag
     5101 cgttccatat ctctatagcg cagggcctgc tcttgttcca atctctcata gagtttttcc
     5161 ttcaaatagg tatccaattt cttgataacc cccataaaaa agagtaggta aaagactagg
     5221 atgagatggc gaacagtctt tgattgaata ctttgttcat attcaaaaaa agacagactt
     5281 tccatgacta gatagtagcc acccattatc cagttaatct gagtcaggga cttttgaaag
     5341 gctttatcga gaatctcctt tctcaagcta gtaaaatcgt agtccaacca tttcaaaaaa
     5401 gctagagaaa tgaagaaatt gaaaattatt atacataacc cagtaaatga gtagccatca
     5461 tatacttgcc cttgtcccaa aaatggaagc acaaaatagg agactcctct ataaaagaga
     5521 ttcaccaata tcattggaaa gagaccataa aagaaaagga gttttttagg aagccctctc
     5581 aataataaga aagataagcc tatgccgtac aagggttcca taaaataaga taggtaaaca
     5641 tttcctacta tatagctaat catcacaaaa acaaaggcca acagtatctt aaaaagaaag
     5701 gccttaaaaa tcctctcgaa agtaagatca attccatcca ccttaaagaa gatgacaatt
     5761 tctagtccat tagtaacaag tgtatacaac aatatccaag caatgttcat aaattctcct
     5821 agctcagtgt aatttattga tggcctcaga cacttccctg accttataac gggcgattag
     5881 acaacttcca ccattgggaa agaaaagcag tttttctttc ttatccaaat gcaccacatt
     5941 tgcaggattg atgagaaaag agcggtggca ctgcaagaga cggggctcct gcttgaaaac
     6001 ctcctctaaa ctcgctgtaa attccagcct gtctgtcttg gtatagagaa taacacgatg
     6061 ggctctgggc gacgtttcga gatagtaaac ctctttaaaa ggatactgaa attgggcaaa
     6121 ttttgattta aagtaaaagc aatcttccgc cagactttta ctatcttgac tattggcata
     6181 gaggagggct gtctcgatcc gagattcaaa ctcctctgct gacaaggcct tatcaatgta
     6241 gtccaaagca gacacttggt agcgaaagga caggggcata aactccgagt gagtcgtcac
     6301 aaagacaatc agggcataag gatcccgatc ccgaatcttt ctagccactt ccagtccctt
     6361 catctcttca tttcgaatct caatatccaa aaagaatagc tgatgggccc ccttcttatg
     6421 cacttcagcc agcagttggt ccggcttgcc aaatacctca aaagagctag gaatgatatg
     6481 atgtgctttc aaaagtttct caatcgtcgt ttcaattcta gtctgttggg aaaaatcatc
     6541 ctctaaaaca aatattctca tcttcttact ctccctcttc caaccatttt tgacgaattt
     6601 ctctatagcg acgtctggca tacttgacag cgtattgacc ttcttctccg agaaaaagga
     6661 ttctttcttg aaaatcaatc gattttaatt tatcaagatt aaccaaacaa tttcgatgac
     6721 atctcatcaa ggtttcccca cattggttct caagattact taaattttga agcatattaa
     6781 aactagcttc ttctgtaaca atctgtacgg tatgggcttt agttggatgt gtttggatat
     6841 agtagatatc gtcgatgttt actttataga ctgttttctg cgtctgaata atcatatatt
     6901 tcattggatt ctcctcctaa gaaaagaata acagaaaagg tctagcccaa gcacacccaa
     6961 tcctaaacag tctttgaaaa ctcctaaaag gattattttc tacaacatca tgatgagctt
     7021 acaactattt tatcataaaa ttcactcagc gcaattcaag acatttcaat gacaattaaa
     7081 gatttgaata aaaaaaaaga attaaaagtg ataaactaac agtatcaaaa cacagttgcc
     7141 agaagtaaga ctggcaccca gattaaagga ggcattccca tgacagatac agaccccatc
     7201 aaaagagctc atactttgat tactgactta aacaaagcct accaagcatg caaacaggca
     7261 agcgctgacg acgtccgctt tcaggagcaa ttaaactcta ttcttggttt tctagccaag
     7321 gctgaaacag tggataatcg attcttgatt gaactagaaa aattttacca gacttccagt
     7381 cttctcatgg gactcagcgc tcttgatcca gatgctccaa ctcgcgccgc ttggcgggcc
     7441 tatgaccgct tccactttga ccaagtcaag accaagttaa tactcaatga gaatcaaaga
     7501 gcaaactagg aagctagccg caagctgtac ttgagtacgg taaggcgacg ctgacgtggt
     7561 ttgaagagat tttcgaagag tataagtctc tacggaccaa ccattatcct gtagtaacaa
     7621 aaaagaagct gagacaaact gaactcaact tcttttttag tgtcgtatca gcaatgttag
     7681 tcctgcatct gacgttttac ctgataaggc aaatacaaga ctagtaaaac caaaccagct
     7741 cctagtaagg ctagaagggc tagtttttct tggaaggtaa tcaacccaac aactaattcc
     7801 acaagtaaaa caaaactgat caatcctcga actacctcct gtaagccttt ttccttttgc
     7861 cccttgtcca gcggaaagag ttgggtcaaa tactggtagt caaaggcatg atagagggcc
     7921 agcaactgga aaagcaagag gtagttaaag agaactacca ctgctgtcgc aatccaagat
     7981 tgctcgataa agacctgcgc cagcaaagaa agcaagagca gacggagact aagggcaaaa
     8041 aggtctccat ttcgcaaata agaacgcaga tagagatttt gccaaatctt cccagacacc
     8101 ttctgaacaa cctttagaat aaagtccaga taggcacgac gcttgacgct gtttgaaatt
     8161 cccttgacct gcgtaaagag ggcaaagaaa cgaagcaaga cttgcttacg cttgctttct
     8221 tgagaaataa catagtccca gtccagtcca gtttcagtga aaaatttgct ggccttttga
     8281 cgaaagtgga aatattttcc tacccccaat aaaagcacat agagcagaaa aactggcaag
     8341 ccataaccca ttgctaaaaa taaaggtgca aataacagca agaaaagggt ctgtacaaag
     8401 agccaaaaga ctagggaaat gccagtttga cgcttgagat ggagcttgat ttcctcttct
     8461 ccaactaaga gaaagagctt gtctggagcc tccatatagg tggcaattcc tccccaaagt
     8521 aaaagtaaaa cagacgtaat tcctacaaac aaaaggatag gccaatgatt ttcaggaaaa
     8581 tgttgtaaga gttgactgta ctggtaggct agaaaaccca acaggacaag caggaacaag
     8641 acaaagtggt cattgagcac atagcgcaga taaccaagac actccttacg aaaggcctgc
     8701 tttctcttta aaaacaagtc tttcatagat cctcctcttt ggtcaaagcc aagtaaatat
     8761 cattcaaact agcctcaggc atatcaaagg cttcacgtag ttgcaggaga ttgcctttgg
     8821 cacgcacctc tcccttgtga agaatgacaa aggcatcaca catcttctcc gccgaatcca
     8881 gcacgtgggt actcatgaga atagacttgc ccttttgctt ctccacttcc aaaagctgaa
     8941 tcaaatcaga aatagccagc ggatcaagac caaggaaagg ctcatccacg atgaaaagac
     9001 ttggatccac cacaaaagca cagataatca tgaccttctg cttcatccct tttgaaaaat
     9061 gaacagggaa ccagtctaat ttttgttcca aacggaacat ttttaacaag ggctctactc
     9121 gttcgaaagc cactttttgc tcaataccgt aagccatagc aaccgtttcg atatgctctc
     9181 tgagggtcaa ttcctcatac agactaggcg tctcaggaat gtagccaatc tgcttgcggt
     9241 agctagtcgc atctccttgc agagtcaggc cattgatatt gatggagcca ctataaggtg
     9301 ccaacagacc gataatctca ttgatcgtcg ttgattttcc agcaccattg agaccaatca
     9361 aaccgaccaa ctgcccactt tcaacagtaa aggacacatc tttcaaaaca ggaacatgaa
     9421 catagccacc tgtcaggttt ttaatttcta acatattttc tccaaatctg gtataatgta
     9481 gctatattat atcaaaattc agtacagtag aggtagattt tatgtcagat tgcatttttt
     9541 gtaaaatcat cgcaggggaa attcctgctt caaaagtata tgaagatgag caggtccttg
     9601 cctttcttga tatctctcaa gtaacactag gacacacctt ggtcgtgcca aaagaacact
     9661 atcgcaatct tttggagatg gatgctacga gcgccagcca actctttgcc caagtaccaa
     9721 aagtagctca aaaagtcatg aaagtcacta aggctgctgg tatgaatatc atttccaact
     9781 gtgaagaagt cgctggtcaa acagtttttc atactcacgt tcaccttgtg cctcgctaca
     9841 gtgctgacga tgacctcaag attgatttta tcgcccacga accagacttt gacaaacttg
     9901 ctcaagtcgc tgaaaccatc aaaaacgctt aaggagttgc tatgaaatta tccaacctac
     9961 tgctatttgc aggagctgca gccggaagtt atctggttac aaaaaatcgc caaaccatca
    10021 cagatgaagt cttgaatacc actgaccgcg ttcaagctat caaggacgat gtggatatta
    10081 tccaaaacag cctgcaaatc attaaccagc aaaaagaact tatcaaggaa taccaagaag
    10141 acttgactta caagtttaag gtcttggaaa aggatatcca gactagacta gctgtgataa
    10201 aagaaatgca gggaactgaa gataagtaaa aagagcccga cggctctttt ttaaaacgta
    10261 aacaagttat agtgaattga atttagaata gcacatcata gtttctaaag cattgttaga
    10321 gattacttta aaatcctctt atcaatttgt tcatattcta tttcaatcta ctatatatat
    10381 cccatcaact atgcatcata atttaggtaa ctcatactca ataaaaatca aaaagcaaac
    10441 tagaaagcta ggcacagact gctcaaaaca ccgttttaag gttgtggata gaactgacga
    10501 agtcagctca aaacactgtt ttgaggttgt ggatagaact gacgaagtca gtaaccatac
    10561 ccatgacaaa ccgacgctga cgtggtttga agaaattttt gaagagtatc attcaccatt
    10621 tcacaactaa atcgaagact ttctgccgta atgaaaacac ctgaaacagc ttggtttcag
    10681 ctgtccggaa actttaagac ttaggttcaa agtttaggta tggaacttcg aaggaggtcg
    10741 ctaccgtccg tcattactta ggaaagtctt aaaaaatcta taaagtaaaa agagcccgac
    10801 agctcttttt gtttatactc aatgaaaatc aaaaagcaaa ctaggaaact agccacgggc
    10861 tgctcaaaac actgttttga ggttgtagat acgactcaca tatatacggc aagccgacgc
    10921 tgacgtggtt tgaagagatt ttcgaagagt attattctcc atcaaaggca tctttaatat
    10981 ggtcaaagaa gccttttttc tttggattta ctttcaagtc accagcagcc gcgaattctt
    11041 tcaaggctac tttttggcgg tcgttcaagc ctgtcggtgt tacgacatta acagtaacgt
    11101 attggtcacc aactgcaccg ccacgaaggc tcggtgcccc cttactacgt aggcggaatt
    11161 tcttaccagt ctgagttccc tctggaataa ccaattcaac atcaccgtga acagttggaa
    11221 tatctactgt atcaccaaga gccgcttgga caaagttgag gttgagattg tagaagatag
    11281 tcgttccttc acgttcaaac ttgtcgctag cttctacaga aactactaca tacaagtccc
    11341 cataaggtcc accgttaaag cctgcttcgc cttgaccagc gaggcgaatt tgttgacctg
    11401 tttccacacc agcagggatt ttcacatgta cgctatgagc ttgtttctca tgacctgttc
    11461 catgacaggt tgtacatgga tatttgattt cttttcctcg accgtgacag acatcacagg
    11521 ttacttggcg acgcatcata ccaagaggag tctgcgtatc gacgttaatg acaccagcgc
    11581 catgacagcg tccacaagtg actggacttg tccctggctt agcaccagag ccattacatg
    11641 tacgacagcc agcttcacga tgatacttaa cttccttctc agttccgaag atagcttctt
    11701 caaaggtcaa attgacacga tactggagat catctccttg gcgaggagcg tttggattgc
    11761 gcgaagaacc gcctccgccg aagaaacttg agaaaatatc ctcaaaacca ccgaagccac
    11821 ctgccccatt gaaaccacca gctccaccaa aaccaccatt ggcgcctgca gcaccatact
    11881 ggtcataggc agcacgtttt tggtcgtcac tcaaagtctc ataggcttct tgaacttcct
    11941 tgtacttgtc ctcagcacca ggctccttgt tgatatctgg gtgatatttt ttggaaagct
    12001 tacgataagc ctttttgatt tcgtctgccg aagcgttttt ggataccccc agacgatcat
    12061 aaaattcagt attgttcata attcaagata caaagggcgt caaacggaca cagccatcta
    12121 ggagttttga cgacggacgc ttacgtccta gaaaaaacta tctttttggc acagtccata
    12181 gcccgtgttc agttacgaac acccttattc ctttctattg atgttctgaa tcgaaccacc
    12241 atttaggtcg ggttcaattc ctttctttta tatttagtaa gaaattctga aacccgtgtt
    12301 cagttacgaa cactctttgt tcctttctat tgatgagaca aaacccaaac aaatgagatt
    12361 ccgtcccttt ttatcgaaaa acagaggctg ggttgcaacc tctgcaaata agtaaattaa
    12421 ctttattttg attttaaata tcctataagt acagaaatat ttatatattt ttgtctcttc
    12481 ttagtatttg cctatataat tccaattgat cataagaaat acaactactg tccaaatcat
    12541 accaattatg aacggtatta caaatttata tgtttgtatt tctaatgata tcatagctgg
    12601 taccatcgcc gatacaaatg cagacaacaa tactgaggta acaaaattcc ttaccgcttc
    12661 atcatttttg atacaactat catgttcagc taatataatt tctctatatc tagctaaata
    12721 ttctattgga atattatcaa taaaatcttt tgatgttcta tagttatcag cttgattaca
    12781 aaaatcatta taaacttttc gtgtattttt tagatactct tcatccaata cactcatctt
    12841 acttttccgt aaactctccg tctacgacgt catcgcctgc gtttcctgtt gcttgtgcgc
    12901 cttctgctcc ttcttgagct tgttgcgctg ctgcggcttg ttcgtagagt ttaacagcaa
    12961 gtccttgagc tttttcgttc aatgcttcaa gttttgcttt catgtcgtcc aagttgttgt
    13021 cttcttgagc tttcttaagg tcatcaaggg cagcttgggc agcgtcacgt tctgcgtcga
    13081 agcctttacc ttcagtttcc ttgattgtct tttcagtcgc aaagattgct tggtctactt
    13141 cattacgaag gtctacttct tctttacgtt tcttatcggc ttcagcgttt gcttctgcat
    13201 ctttcatcat gcggtcgatt tcttcgtcag tcaaacctga gttcgattgg atgacaatag
    13261 tttgttcttt ttgagttcca aggtctttgg ccttaacaga cacgataccg ttcttgtcga
    13321 tgtcaaatgt tacttcgatt tgaggaattc cacgaggtgc agctgggata tcagtcaatt
    13381 ggaagcgtcc aagagtcttg ttatctgctg ccattgggcg ttcaccttga agaacgtgga
    13441 tatcaacggc tggttggttg tctgctgctg ttgagaagac ttgtgattta gatgttggga
    13501 ttgtagtgtt gcgatcgata agttttgtaa atactccacc cattgtttcg ataccaagtg
    13561 acaatggcgt tacatcaaga aggacaacgt ccttgacatc accagtaatc acaccacctt
    13621 ggatagccgc acccatagca actacttcat cagggtttac tgatttgttt ggttctttac
    13681 cagtttcagc tttaacagct tcaacaacgg cagggatacg agttgaacca ccaacaagga
    13741 taacttcgtc gatttctgac aagctcaaac ctgcatctga aagggcttga cgaactggaa
    13801 cttttgtacg ttcaacaagg tcacgagtca aatcatcaaa tttcgcacga gtcaaagtca
    13861 tttccaagtg aagaggtcca gcctcacctg cagtgataaa tggcaagctg atttgtgttg
    13921 aagttacacc agaaaggtct ttcttcgctt tttcagccgc atctttcaaa cgttgcattg
    13981 ccatcttgtc agtagacaag tcgataccgt tttctttctt gaattctgct accaagtggt
    14041 caatgatttt ttggtcaaag tcgtcaccac caagtttgtt gtcccctgca gttgacaata
    14101 cgtcgaagac accgtcaccc aattcaagga tagagacgtc gaatgtacca ccaccaaggt
    14161 caaataccaa gattttttct tctttgtcag tcttgtccaa accataagca agagctgctg
    14221 cagttggttc gttaacaata cgttctactt caagaccagc aattttacca gcgtcttttg
    14281 ttgcttgacg ttgagcgtcg ttgaagtaag ctggaactgt gataacagct ttggttactt
    14341 tctcaccaag gtagtcttca gcgtagcctt tcaagtattg aaggatcata gctgagattt
    14401 cttgtggagt gtattctttt ccatttgcag aaactttttc agaagttccc atcttagatt
    14461 tgatagagat aactgtatct gggtttgtga ctgcttgacg ttttgcagca tcaccaacga
    14521 tgatttctcc gtttttgaat gagactacag atggagttgt gcggtttcct tctgggtttg
    14581 cgatgatttt gctttcagtt ccttcaagaa ctgcaactgc tgagtttgtt gtacctaagt
    14641 caataccgat aattttagac atgtgttttt ctccttgtta gttaattttc tatttttctt
    14701 tttgatactc ttcttaagtg gcgacgccgt cagagcttga cttcgtcaat ctctttgact
    14761 aaactttgag cctaaggtct caaaatttgc gcaaatagcg ccacggcgaa gagtatcgtg
    14821 tttggttcgc ttcgctcact attacaaagc caaacatatt ttatctttct tcatagttta
    14881 ttgtcgtttc ggacaagttt tctattatgt aaattgcgac acgaggagtc gaaatcgatt
    14941 ttatttcgac gacgagttag taaggaagct aggcaaacgc cataacgatt gccgttttct
    15001 gacgagttgc tttagctacc gtcagaattg ccgaatcgaa cacgccctaa gctctgagta
    15061 aaaaagatag attttcttgt gttcatcgaa cacttcgtca atctcctatt tttactgcga
    15121 gctttttacg ggctttatat cttagttata caccactacc attgctgggc gtaggatgcg
    15181 gtcatggagt ttgtagcctt tttgaaagac ttgagcgatg gtatctactg ggtgttcatc
    15241 gtctgctggg agagtttgga tggccatatg gtagttatgg tcaaattcgc catctgctgc
    15301 gatttcttca attccttctt ctttcaaagc gtgaatcaag ctttcttgca ccatccccaa
    15361 gcccttcttc acatcatctg tcaaaccttc aactgcaagt gcacgctcaa ggttgtcaag
    15421 agatggtaag attgcttttg ccaagtcctg gctacgataa cgttgcaagt tttgacgttc
    15481 ttcattggca cggcgttgga tattttgcat ttctgcatga gcgcgaagat atttgttttc
    15541 gaactcatct gcacgttcat ttgccaagtc caactcagac ttttcaggag ttgtttcttc
    15601 agctgtttcc acaacttcct cttcttgaac ttcttctact tcttcatttt ttatatcttg
    15661 ggccatgttc gcctccttta atgatttcaa tcttaatgta cttcgtaatg attactgctg
    15721 aggtagcggt aaaaatctgt caacttcatg gtcaaaacac ggttgaccac attgacttga
    15781 ttgattagct gttggtaatc cagattaact ggaccgataa tggctagaat tccaactccc
    15841 cgataaggaa tgaggaactt actactaatc accgctaggt cagctaaaca ggactcttga
    15901 ccgtctgcaa cacgaacatt ttgcatctga tcctcacgca acccctcacg aatctccaag
    15961 gccacctttt gcggttggtc aaagaactga taggcttcta gattggcaaa attcaagaga
    16021 tggaccttgc ccgccatcac aatgttttcg ttgaacattt ccttaaagat gtgttcaaag
    16081 agatcgatga cattatccgt tgttgtaaag taacgctgga taatctgcgg aatctccgtc
    16141 cgaatcttgt agtgaatatc taaaacggtg tgaccgagga aacgttcctg aatgatgctc
    16201 ttcagtttca gcaaatcctc ctgcaagaag ttccttggaa tcagaaactg actagtaacc
    16261 gttcgcgact cgtctagggt aaataccgcc aaggctgtat gttgccccaa aacaacgata
    16321 tcaaaggctg tcaaacgttg cctgctcggc tcaacatcca gtgccactac cgtacagcca
    16381 ctcaggtctg ttagcaagtt agcagcctct tgcagaatat cctccaattt gaagaattcc
    16441 tgatcaaagg ctttgacaat ctcatatacc tcattttcag ccagccggtg aaaatccagt
    16501 gagtgtttca catagtactg aaaaccagca acacttggca tccgaccgct tgaagtatga
    16561 gccttctcaa gcaacccttg tttttctagt tccgccatgt cattacgaat ggttgcactg
    16621 ctagagttaa tagactcttg caaggctttt gatccgacag gttcgtgcgt tttggtaaag
    16681 atgtcaataa tcagatttaa aatatcctgc tgacgctctg taaccatctc atcactcctc
    16741 tctgtctgat caattagcac tcaatgcatc caagtgctaa cttatgattc tattatacac
    16801 ccttaaagga gaatgtcaag acaaaaactc aaaaaattag cactctttta tcaagagtgc
    16861 taaaaatcaa tctgaagacc tagaaccttg ctattcatct actcggtatt tcctttttag
    16921 tctgaaataa ccatgaatta gataagaaat cagaaaagca tataaaacaa aaataagaaa
    16981 gaaagacata gaaagatcct ctctaaacaa actcgtacaa acaacaagac cacctaagaa
    17041 agtagttgta caggaacgaa gtctagatcg cataacttcc catttgagcc ccttactcat
    17101 atagacttga tcctctggaa gtaaatcaga agacgattta cgaagaatcg aacaagaacc
    17161 tgctcctatc aattcccaac ctctatctct aaaatcttgc agttcatgct tatatttttt
    17221 aagaaatcta gaatcataga tacggtagat gacatcgtct ggttgacatt ggtcaaaata
    17281 gaacaaacca aaacgactcg ttctatacct ccaacctttc aaatgcatct catgtaaatg
    17341 ttcttcttcc ttgtccaaat caacaatggt gaaaatccga aattctactc tgctattcat
    17401 tgtcttaccc caaaattaga aaacatgcct ggcgttattt attagataat tctttccact
    17461 tttgactcaa tctccaaaaa atataagaaa tctgaatcgc aaaaactatc aataaaaccc
    17521 aatctattat gaaaatcaaa aacactttcc aactgaaaga actacctcca gtgacaaact
    17581 ttgagaaaaa cggtagtaga gctaaaaaga gaagtaaaat aggaagcatc cgcattgtta
    17641 aaatccgttt ggcataaaaa atctttattt aaactaaaat attatagcaa aatttacgcc
    17701 agtttttgaa cggctgatgt agatatttta tactttcaaa atgtttaaat gtgattattt
    17761 atttttgaaa aatagctcac cagcccgact gaaagtgctt atagaatgat aataagtcgc
    17821 ctgccgaaaa cagcggaaaa tagcggtgtt atgcggagat aatctgacgc gatgcgaaag
    17881 tatattgcat acttattttc aacaatttag cagagtattt ttataagtgt gatataatag
    17941 aagtataatt tgttctgata gtttatttta tggagaagta gatttttaga atgcggaggg
    18001 ttcaatatgg ttgagtttat aaagtctaag aaagaaatga gtgaggagga tattaaagca
    18061 aatttcatca ctcctgctat tgtatccaaa ggatggaaaa atggtgagca tatcgcttac
    18121 gaagaatact tcactgatgg tcgaattgag gttagaggag ataaggctcg tcgtaaagaa
    18181 ggaaaaaaat cagactattc actgtattac caatttggaa ctcgaattgc aattgttgag
    18241 gcaaaggata ataaacacag cgttcgagca ggattacaac aagctattga atatggagag
    18301 attttagatg ttccatttgt ttattcttcg aatggtgatg gctttattga acacgaccgt
    18361 atcacgagag aagaacgtga gctggagtta gacgaattcc ctactcgtga agaattattt
    18421 tctcgtatga cgaaggaaaa aggattgacg tacgaaatta cagaagctat ctcaactcca
    18481 tactatacag acgccttctc aatgaaaacg ccacgctatt atcagcaaat agctatcaac
    18541 cgtactattg aaacagttgc cagaggacaa aaacgagtaa tgtttgtgat ggcaacagga
    18601 acggggaaaa cgttcatggc ttttcaaatt attcatcgcc ttcgaaaagc tggtttggct
    18661 aaacgagttt tattcttagc ggatagaaac atcttagtag accaaacgat ggctgaagac
    18721 tttaagccat tcgaaaaggt aatgacgaaa attacaccaa aacttttgac tgctcctgaa
    18781 aaattaaatt cttttgaaat ttatctaggg ctttatcagc aactaactgg tgaagatgga
    18841 actgaaacac attatcaaaa atttgacaaa gacttctttg atttaatcgt aattgatgaa
    18901 gcgcaccgtg gttcagctaa ggaaaacagt aactggcgta agataattga ttatttcagt
    18961 tctgcgacac agattgggat gaccgctact cctaaagaaa ccaagaatgc ttccaatacg
    19021 gaatactttg gtgagccaat ctatacttat agtttaaaac agggaatcga ggatggtttt
    19081 ttggctccat atcgtgttat gagggttaat ttagatgtgg atgtggatgg ttatcgtcca
    19141 gaaactggaa aagttgatgc taacggacaa ttaatagaag ataggtacta cagcaggaaa
    19201 gattttgata aaaccattgt cattgatgat agaacgcaaa gagttgccaa gtttgtttct
    19261 gattatatga agcaaaacaa tgcacgattt gataaaacaa ttgttttttg tgttgatatt
    19321 gaccatgccg agcgaatgcg tgctgcactt gtaaaagaga atctagactt agtccaagaa
    19381 gactatcgtt atgtcatgca agtaactggt gacaacgctg aaggaaaagc tcaactggat
    19441 aactttatgg atgtcaattc taattttccc gctattgtaa caacgtctaa attattaacg
    19501 acaggagtta atgctaaaac atgtcgtttg attgttttag actctaatat ccaatccatg
    19561 actgaattta aacaaattat tggtcgtggc acacgtcttt atcctcaaaa ggggaaagaa
    19621 ttttttacga ttattgattt tcgaaatgtt accaatttgt ttgctgaccc tgattttgat
    19681 ggtgagccag tgaaggtgct ggaaacaggt gcgaaaacag tcagtggttc tacgcccggt
    19741 ttcgtagatg aggaaggtga cccagtagaa aaatatatcg ttacagacaa gcaggttacc
    19801 attcttaatt ctactgttca agtattggat gaaaacggga aactgattac cgaaagcctg
    19861 accgactaca ctcggaagaa tatcttaggt agctacgcca ctttgaacga ttttatcaca
    19921 gtttggcata cggcggataa aaagaagctt atcttagacg aactttataa aaaaggagtt
    19981 tatctagatg ctattcgaga gtcggaggga atatcagaac aagaaatcga tgattttgat
    20041 ttactcctaa aacttgccta tggtcaaaaa gaattaacca aaacggaacg tatcaataaa
    20101 ctcaaacaaa gcggatattt atataaatat agtgaggaag cgcgtgctgt tttggaaatt
    20161 ttactgaaca aatacatgga taaaggtatt ggagaactcg aaagcattga aacattaaaa
    20221 cttccagaat ttcagatata tggtggaacc ttcaaaatca tcaatactta ttttggagat
    20281 aaaaaacgat atttacaagc aattaaagaa ttggagcaag agctatttac agtagcttaa
    20341 tgaaaggaaa gtatgtcaat tacatcattt gtaaaaagaa ttcaagatat cactcgaaac
    20401 gatgctggtg ttaatggtga tgctcaacgt attgagcaaa tgtcttggtt attattctta
    20461 aaaatttatg atagccgtga aatggtttgg gaattagaag aagacgagta tgagtcaatt
    20521 atcccagagg aattaaaatg gcgaaattgg gctcatgctc aaaatgggga acgggtattg
    20581 acaggcgatg aattacttga ttttgtcaat aacaagttat tcaaagagtt gaaagagctt
    20641 gaaataactt caaatatgcc tattcgaaaa acgattgtta aatcagcttt tgaagatgcg
    20701 aacaactata tgaaaaatgg cgtcttgtta cgccaagtca tcaatgttat tgatgaagtt
    20761 gatttcaata gccctgaaga tcgtcattcg tttaatgata tttacgaaaa aattcttaaa
    20821 gatattcaaa atgctgggaa ctcaggagaa ttttatacgc cacgtgcagc gactgatttt
    20881 attgccgaag ttcttgaccc aaaacttgga gaatcaatgg tagaccttgc ttgcggaaca
    20941 ggaggcttct tgacttcgac tctgaaccgt ttaagtagtc aacgtaaaac tagtgaagat
    21001 accaaaaaat ataatacagc tgtttttggt attgaaaaga aagcatttcc tcatctttta
    21061 gcagttacaa atctgtttct tcacgaaatt gatgacccta aaattgttca tggaaatact
    21121 ttggaaaaaa atgttcgtga atatacggat gatgaaaaat ttgacattat tatgatgaat
    21181 ccaccttttg gaggggcaga attagaaaca ataaaaaata actttccagc agaattacgg
    21241 agttctgaaa cagctgattt atttatggct gtcattatgt atcgtttgaa agaaaatggt
    21301 cgtgttggag ttattttacc tgatggtttt ctatttggtg aaggtgtaaa aactcgcttg
    21361 aaacaaaaac tggtagatga gttcaacttg catacgatta ttaggttgcc tcatagtgtc
    21421 tttgcaccgt atacaggaat ccatacgaac attcttttct ttgataaaac aaagaaaaca
    21481 gaagaaactt ggttttatcg tttagatatg ccagatggtt ataaaaattt ctcgaaaact
    21541 aagccgatga agtcagaaca cttcaatcct gttcgtgact ggtgggaaaa tcgtgaagag
    21601 attctggaag gtaagttcta caaatctaaa tcatttacac ctagtgaatt ggctgagttg
    21661 aattataatt tagaccagtg tggctttcca aaagaggaag aggaaatctt aaatcccttt
    21721 gagttgattc agaattatca agcggaaaga gcaactttaa atcataagat tgataatgta
    21781 ttagctgata ttttgcagtt gttggaggac aaataatgac accagaacaa cttaaagcaa
    21841 gtattctcca aagagcgatg gaagggaaat tagtgccgca aaatcccaat gacgaacctg
    21901 caagtgaatt attaaagaga attaaagctg aaaaagaaaa acttatcagt gaaggaaaaa
    21961 tcaaacgaga taaaaaggaa actgagatat ttcgtggtga tgatgggaaa cattatggga
    22021 agtttgctga tggaagcact caagaaattg atgttcctta tgatattcct gatacttggg
    22081 agtgggtgag gttttctaca ttggttgaaa ttgtcagagg tggctctcca cgaccaatca
    22141 aagattatct tacttctgaa gtagatggaa taaattggat aaaaataggt gatactgaaa
    22201 agggtgaaaa gtatataaat aatgttaaag aaaaaatcaa aaaatcagga cttaacaaaa
    22261 ctagatttgt aaaaaaaggt acatttttgt taactaattc tatgagtttt ggtagacctt
    22321 atattttgaa tgttgatggt gcaatacacg atggatggtt ggctatttcg aactatgaaa
    22381 actcattaaa taaagattac ctattctata ttctttcatc aaatgtagtt tattctcaat
    22441 ttctatctct aattagtgga gctgttgtga aaaacttgaa tagtgataaa gttgcttcta
    22501 ttcttatccc tctcccccca ctatccgaac aacaacgcat aatagaagca atcgaatcag
    22561 ctttagaaaa agtagatgaa tatgctgaaa gttataatag actagaacag ctagataaag
    22621 aatttccaga taaactaaaa aaatctattc ttcaatatgc tatgcaagga aaattagttg
    22681 aacaagaccc aaatgatgaa tcagtcgaag ttttacttga aaaaatacga gcagaaaaac
    22741 aaaaactctt tgaagaaggc aagattaaaa agaaagattt ggacatttct attgtttccc
    22801 aaggagatga taactcttat tatgaggaag taccttgtga aataccagaa agttgggaat
    22861 gggtgaggtt aaacgatata acctcctaca ttcaaagagg gaagtctcca aaatattcaa
    22921 atattcctat ataccctgtg attgctcaga aatgtaatca atggtctggc ttttctattg
    22981 acttagcaag atttattgat cccgaaacag ttcatagtta ccagaaagaa agattactaa
    23041 gagatggtga tttaatgtgg aattcaacag gtctaggtac gttaggacga ttagctattt
    23101 accatgaaaa taaaaatcct tatggctggg ctgttgcaga tagccatgtt acagttataa
    23161 gagtcttatc gggagttatt aattgccatt tcatctataa ttttctttcc tctcctattg
    23221 ttcagtcagt tattgaagaa aaagcgtcgg gtagtacaaa acaaaaagaa ttattaacga
    23281 aaactataaa agaatattta atccccctcc caccccttcc tgaacaatcc agaatcgttg
    23341 ataaaatcga acaatttttc gcccatattg acgcactaat ttagtataat agttttacgt
    23401 gcggtggaat ttctatcaaa cataagatag gagttttacg ttgaaagatt ttgaattaca
    23461 tttgaagaaa gctggtttag ctgaaaatac tgtgcgttcg tacctttatg gtgttcgatt
    23521 tttcttggag aattacgaac tcaaaatgga agatttattt gaatacaaag gatatttatt
    23581 agacaatttt aaacctaaaa ctgtcaattt gcgactacaa ggagtaaata aatatctggc
    23641 ttttattggt catgatgatt tgaaattaaa atttgtcaaa gtacagcaaa aaccgttttt
    23701 agaagatgtc attagccatg ctgattacct ttttcttaaa cgtagtttga aaaaagatgg
    23761 tattttaaaa tggcattttg tcgtttggtt tttaggtgca actggggctc gtgtaagtga
    23821 acttattaaa ctaaaagtag agcatgtaga aatcggttat tttgacattt actcaaaagg
    23881 tggtaaaatt cgtagactgt acattcctaa aaaattaaga aatagttgtc ttagttggtt
    23941 agaatcagaa aatcgccgaa gtggttattt atttttgaat aaatttaacg aaccaattac
    24001 agcaagagga gttgctcaac agttaaaaaa ttatgctgat aaatacaaaa tgaatcctaa
    24061 agtaatttac cctcattctt ttaggcattt atttgctaag aattttttag cgaagtataa
    24121 tgatattgcc ttgcttgcag atttgatggg acacgaaagt atagaaacta ctcgaattta
    24181 tctaaggaaa acagctactg aacaacaaaa tattgtagat aaaattgtta attggtaaaa
    24241 aataacaggt agtcaaactg actacctgtt atttttgtga ttatggctct tattatggga
    24301 atatacctat gaattgggtt gttataaaaa taaaagatat tttttcaata aatacaggtc
    24361 tttcttacaa gaagggcgat ttaagcatta ataataaagg tgttagaatt atacgtggtg
    24421 gtaatattaa gcctttagaa ttttctctgt tggataatga ttactacatt gatacacaat
    24481 tcatctcctc tgagcaagtt tatttaaaac ataatcagct aataacacct gtatcaacct
    24541 ctttagaaca tattggaaag tttgcaagaa tcgataaaga ctatgatggt gttgtggctg
    24601 gtggatttat tttccaatta acaccattcg aaagttcaga gattatttca aaatttctat
    24661 tatttaactt gtcctctccg ttattttata aacaattgaa agcaataact aaactatcag
    24721 gtcaagcttt atataatatt cctaaaacta cactaagcga gctattaatt ccgttagctc
    24781 cttttgagga acaggaactt attactcaaa aagttgagaa actttttgaa aaagtaaatc
    24841 aactttggaa atgattcttt tcatctcttc atgattagaa atagggatta ataattcgga
    24901 gatactggta ctatttaatg ttttcccttt gatagcatct tttgaatcac ctaaagtaga
    24961 gataagtggc aaaaatatca ttaagtaatc tctgataata ttttctttat tagcataggg
    25021 gaatatcgat ataatggctt cattatgagt ggcaggaata tccaatatgg caacttttcc
    25081 aatagataat ttaaagctca ttaataaagt tcctttaggt gaaatgtcta ttttctttga
    25141 ttttaatgct aatttagaaa tagattctct cgtattagtt acataaccag atataggcat
    25201 atctgatata gatacccagg gtatttcagt tccccaaaaa gtagcttcac tgcgtggagg
    25261 agtttttcct attctgaagt taactaggct agcaaattta atatatctcc atgcttctgg
    25321 gatttcatat ataggataag aggttgtttc gtctttgttc
//
LOCUS       DAVKQL010000029        25033 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVKQL010000029 DAVKQL010000000
VERSION     DAVKQL010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25033
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.029"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(199..477)
                     /locus_tag="VW561_001139"
     CDS             complement(199..477)
                     /locus_tag="VW561_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943408.1"
                     /translation="MKKDKEVYDKVCEYLSPHLNKDMEVLELACWFRVITVIEANSYV
                     NIRSSRLLPKFKTQKINSWCAAYNIHFNNGYCKQHTNEFKDSLKSLIL"
     gene            complement(691..2322)
                     /gene="rlmD"
                     /locus_tag="VW561_001140"
     CDS             complement(691..2322)
                     /gene="rlmD"
                     /locus_tag="VW561_001140"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU9943409.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHDLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(2396..3370)
                     /locus_tag="VW561_001141"
     CDS             complement(2396..3370)
                     /locus_tag="VW561_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEU9943410.1"
                     /translation="MRKKLFLTSAAVLWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDVRKIVENTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(3444..4058)
                     /gene="pmp23"
                     /locus_tag="VW561_001142"
     CDS             complement(3444..4058)
                     /gene="pmp23"
                     /locus_tag="VW561_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEU9943411.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVQLNLYIIKCFTLFSTSG"
     gene            complement(4058..5032)
                     /locus_tag="VW561_001143"
     CDS             complement(4058..5032)
                     /locus_tag="VW561_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEU9943412.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSSKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDVSRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(5040..6296)
                     /locus_tag="VW561_001144"
     CDS             complement(5040..6296)
                     /locus_tag="VW561_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU9943413.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYESLSPFKTSGIRIGAAAITARGFGEEESRKVAEFIIKTLKN
                     SENEAVLEEVRSAVKELTDAFLLYED"
     gene            complement(6358..6789)
                     /locus_tag="VW561_001145"
     CDS             complement(6358..6789)
                     /locus_tag="VW561_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9943414.1"
                     /translation="MLRDLQETDVKAICDINQETLGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEQEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(6791..7393)
                     /locus_tag="VW561_001146"
     CDS             complement(6791..7393)
                     /locus_tag="VW561_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEU9943415.1"
                     /translation="MMDRIRQELEKGGAVILPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNVASLEDILHFSKNQPAYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(7377..8216)
                     /gene="prmC"
                     /locus_tag="VW561_001147"
     CDS             complement(7377..8216)
                     /gene="prmC"
                     /locus_tag="VW561_001147"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEU9943416.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASDQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKKVRTLKDQFGQDRMVVVDD
                     GQD"
     gene            complement(8216..9295)
                     /gene="prfA"
                     /locus_tag="VW561_001148"
     CDS             complement(8216..9295)
                     /gene="prfA"
                     /locus_tag="VW561_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEU9943417.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEELAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(9292..9882)
                     /locus_tag="VW561_001149"
     CDS             complement(9292..9882)
                     /locus_tag="VW561_001149"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEU9943418.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRGLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            10005..10187
                     /locus_tag="VW561_001150"
     CDS             10005..10187
                     /locus_tag="VW561_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEU9943419.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            10324..11697
                     /gene="mnmE"
                     /locus_tag="VW561_001151"
     CDS             10324..11697
                     /gene="mnmE"
                     /locus_tag="VW561_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEU9943420.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGVRLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNIRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEANLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLIEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(11744..12679)
                     /gene="dapA"
                     /locus_tag="VW561_001152"
     CDS             complement(11744..12679)
                     /gene="dapA"
                     /locus_tag="VW561_001152"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEU9943421.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKTIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(12735..13811)
                     /locus_tag="VW561_001153"
     CDS             complement(12735..13811)
                     /locus_tag="VW561_001153"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEU9943422.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIASF
                     PGAVLEDDVVHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(14397..14469)
                     /locus_tag="VW561_001154"
     tRNA            complement(14397..14469)
                     /locus_tag="VW561_001154"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14434..14436),aa:Thr,seq:ggt)
     gene            complement(14569..15078)
                     /locus_tag="VW561_001155"
     CDS             complement(14569..15078)
                     /locus_tag="VW561_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEU9943423.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(15076..15172)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            15201..15632
                     /locus_tag="VW561_001156"
     CDS             15201..15632
                     /locus_tag="VW561_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEU9943424.1"
                     /translation="MKKPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            15714..16091
                     /gene="mscL"
                     /locus_tag="VW561_001157"
     CDS             15714..16091
                     /gene="mscL"
                     /locus_tag="VW561_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEU9943425.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(16133..17227)
                     /gene="hemH"
                     /locus_tag="VW561_001158"
     CDS             complement(16133..17227)
                     /gene="hemH"
                     /locus_tag="VW561_001158"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEU9943426.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            17382..18602
                     /gene="pepT"
                     /locus_tag="VW561_001159"
     CDS             17382..18602
                     /gene="pepT"
                     /locus_tag="VW561_001159"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEU9943427.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEA
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSNRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVRSL"
     gene            complement(18645..19274)
                     /locus_tag="VW561_001160"
     CDS             complement(18645..19274)
                     /locus_tag="VW561_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943428.1"
                     /translation="MNKANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDPQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSAKSKDKTALLESTKAILVLNHQIQSKASASEETSPARNAEANGDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENKTTESSTTTGNQEKPVE"
     gene            19239..19460
                     /locus_tag="VW561_001161"
     CDS             19239..19460
                     /locus_tag="VW561_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943429.1"
                     /translation="MDLFQELICLVQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(19731..20743)
                     /locus_tag="VW561_001162"
                     /pseudo
     CDS             complement(19731..20743)
                     /locus_tag="VW561_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(20969..24088)
                     /gene="phtE"
                     /locus_tag="VW561_001163"
     CDS             complement(20969..24088)
                     /gene="phtE"
                     /locus_tag="VW561_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEU9943430.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENRKVPTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
     gene            complement(24298..>25033)
                     /locus_tag="VW561_001164"
     CDS             complement(24298..>25033)
                     /locus_tag="VW561_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEU9943431.1"
                     /translation="HQDSGNTEAKGAEAIYNRVKAAKKVPLDRMPYNLQYTVEVKNGS
                     LIIPHYDHYHNIKFEWFDEGLYEAPKGYSLEDLLATVKYYVEHPNERPHSDNGFGNAS
                     DHVRKNKADQDSKPDEDKGHDEVSEPTHPESDEKENHAGLNPSADNLYKPSTDTEETE
                     EEAEDTTDEAEIPQVEHSVINAKIAEAEALLEKVTDSSIRQNAVETLTGLKSSLLLGT
                     KDNNTISAEVDSLLALLKESQPTPIQ"
BASE COUNT         7138 a         5188 c         4599 g         8108 t
ORIGIN      
        1 atattgattt cattcaatct tacattgatt tagtgatttg ttttagagaa tttgttgtgt
       61 tattccgtcc ttattttgcc ataaattgtt ttaagtggga cctcttatcc ttaccatttt
      121 tacctcctaa ttttgagtat aaaaaagacg atagagtttt taatttctat cgtctgagat
      181 ttacatatta aattttcatc acaaaataag actctttaaa gaatctttaa attcattggt
      241 atgctgctta caatatccat tattaaaatg gatattgtaa gcagcacacc aactatttat
      301 tttttgagtt ttaaactttg gtagaagtct tgaactcctt atatttacat aactatttgc
      361 ctctataact gttatgacac gaaaccaaca agcaagttca agcacctcca tatctttatt
      421 caaatgagga ctaagatatt cacaaacttt atcataaacc tctttatctt ttttcataaa
      481 aggggcatac aacttagcaa atttatccta aaattttttg ttgcctttat caatcatttc
      541 aacgctcctt taaatataaa aatgatccta tcaatcttta tattgagcct attttctaat
      601 tgataccttg caagaaagga ataatagctg ttcctatacc aagtaggaat gtaataaatg
      661 cagaaagtca tatactgtca ttcttttcta ttaaatcatt ttgaagtgtc tcaaagcatc
      721 catgatggct tcttcttttt caggtgtaca ctgtggaata atttgtttat cctttttaga
      781 cttgttgtaa tgttctcgta attctattcc acatttcttt tttatctgtg caatatataa
      841 tgtcgaaact tttaattcaa atttattcca aacatattct ttgatttgag catatgttgc
      901 tttgctctcc gcacttgtca aatccatctc atccagctca atttcaacac ttatgtgctt
      961 atcgacatcg agtttggaca aaagtgctac cgtctcgacg tgatgcgttt gaggaaatag
     1021 atccaccggc tggactttct tcaattcata tcccaattct tggtagagtt tgatatcacg
     1081 cgccatggtt gcgacattgc aggagatata ggcgatgcga tcggctcctg tttgggcgct
     1141 tgctttgata aagctttctg tcaagccctt gcgtggagga tcaaccaaga taacggttgg
     1201 ttgaatccca tctttaagcc aattcttcat agcattttca gctgtgtcac agacatagtg
     1261 ggcgtttgaa atattgttca gctgagcatt ttttttacta ttctcaaccg cttctggaat
     1321 cagttcaaca ccgtagactt ctttgacatg cttggcgact gataaaccaa tggttccaat
     1381 accagaataa gcatcaataa ccacgtcatc tttttttaac tctgcaaagt caatggctgt
     1441 ttgatagagt ttctccgcca tttcagtatt gacttggtaa aaggctgggc cagcgatttg
     1501 gaagtcattt cccaacatct ggtccgtaat atagtcttga ccataaagag tgcgccactc
     1561 cttaccaaaa atcgcattgg tattctggtc gttgatattt tgcatgacag acacaatctc
     1621 tgggaactgc ttgataactt gttcaatcaa ttggtcaaca cgaaaaactt ttggacgagt
     1681 tgtcaccaaa acgaccatga tttgtcctga atagtgacca cgacgcacca caagattccg
     1741 aatcaatcca gactgttcct tttcgtcata aggttttaaa tcaaaacgac ggagcaggtc
     1801 tcgaagagct actacgactt ggtcaatgac aggatcctgg ataaagaaat cttcaagggg
     1861 catgaggtcg tgcgaattct tacggaaaaa tcctgtttcc aagacaccat tcactcgacg
     1921 aacgggcacc tgcgccttat tgcgatactt gactggatgt tccataccaa gcgtttcagc
     1981 aacttctaca tctgcaattc cagcaatctt gtagagactg tccttgactt gcttggtttt
     2041 aaacttgagc tgttctggat aagaaaggtg tcctaaatcc gcgattcctg aacgcaggta
     2101 agccaaatct agatcttgat tacggtgtgg tgactggaca aggtattttt caacttttcc
     2161 aaagccaatc tttttattga ccttgaggac acgcatgaga attttttcac tcggtaaagc
     2221 attctctaca aaaaagacca aaccatctac cttggcaact cctgcccctt catgggtcaa
     2281 atcaacaatt tcaacttcta caatatcatt tttctttaac attcttttct ctttctattg
     2341 gccgacaaaa aagacggcaa cgttacggtt accatccatt ataccatgaa atttcttaag
     2401 aaccaaaact cttgaagaag ttgacaaggg cttgccagag ggagcttaag aagttaccac
     2461 cgtcctctag cgccttatcc gcatcaaagt taaggttgat atttttaaaa ctgtcgccag
     2521 cttgtgatac gatgctttgt ttaaggtcat ttagggtttt agtgaaatct gcattgctga
     2581 ggatatcact ctttgagaga ttcaaggcaa aattgatgat gatattgatc tggtttcctg
     2641 ttatgacctg atcaagtttg taatttttta aggtattttc aacaatcttg cgtacatctt
     2701 cttctgtcag atttcccttg ctttctttag ctttggcgag tcctgacttg atatcagcta
     2761 gggcaacgtt taatttatta gcatcatagc ctgatttgtc cttgttttca gcattgatat
     2821 ctgacaaagc ctttagctct tcttgagcca aatctttatt ggcttgcggc accttggctc
     2881 cattagcttc tagcgaatag tagatcccag ccaaagcact ctcacctgta actggaatag
     2941 gggctgctac agtgattttg gcatgttcca tacccagcgt tactgctgcg tttcggtaca
     3001 tatcctgagt caccttagtg atattttctg gtgtttcaat cttgacctca agtggcgatt
     3061 tgtcacctag cttttgaatc ttggctgatg aatacaactg taagctagag tcattggcca
     3121 cattcatgat tttagaataa acatcaggtg tcatggtctt gagttctttg gtatctgttg
     3181 aggcattgta gcccagtttt ttaagagttt gatttttttg gtcttcagat agggaggaac
     3241 ctaggacata ttcaggttgg acataggttt catcgataac tttttgaaca tctgttgctg
     3301 catggacgct attcatagct gttactgccc acaagaccgc agcactagtc agaaagagtt
     3361 tctttctcat agggaatttc ctcctttacc tttctagagt aatatatcta tcttaaagaa
     3421 aacttataac aaaaacacct gaactagcca gatgttgaaa agagagtgaa acatttgatg
     3481 atgtaaaggt tgagttgcac ctgtctagaa taataatagt ttcctccatt tacatagagt
     3541 tcggcaccgt gaaaaatgga aatgggatga atataactat aagtctttcc agtcgtatta
     3601 ccaagcaagg gggcaacagt ctcacgagag tactgtttgg ctagagccaa ggtattttcc
     3661 ttgccatttt gggcgataaa atcgatatag gcaggtccaa aattataggc ttgaacagct
     3721 gtccagatat ctacaccctt cttctgcgcc agatagagat tgcctgtcag agtttgaatg
     3781 ccttgccgaa tgctagaggc attatcattg atggtgttgg tggaaccact tgcagactca
     3841 ctagactgca taacatcgcc ttcttttcct tttgtttcag tataaatcat agcaagcaca
     3901 agctcttcgt ttgctggggt gtctttttca ctcaatattt ctcgcaccat gggttgatag
     3961 gtcatgactt gtttgacatc ttgatgaacg cggtaagctt tatagccagc aaaaaggaag
     4021 actgctagta caagcactct tcgaattcgt ttaaacatta tttactttgg atatcctcga
     4081 tatttttgat taagatagag taggttccat tttcgttttg gataaactca acagactcgg
     4141 cgtcttgata gacgttattg ggaacgatga gctcaattcc atttgataag gagagttttt
     4201 ggttttcaaa tttctttaat tggcgactga catcaatttc atcaaattga acaggttctg
     4261 gtacggcttc tttgacttgg tcaataaagc tcaaacgagc cgtcagattg ttgtcaaaaa
     4321 ggtcattagc caatttctca ggtgacaatt cattgctttc ttctaggttg ttgaaaatag
     4381 ctgatttgac cttggattga aattgaaaat catctgtgtt aaaagattca gcaattctct
     4441 gggctgtttt ttccagttcc ttgatagatt ttttaggaga aatcttagga gcgacagcaa
     4501 gaagattatc tgaaaaatag ttcaaaaaag tcccgttgta cttgattcgt ttttcaatca
     4561 ggtgatactt gctactctga agattgacca ccaaggcctc atcagctcct gttccaaatc
     4621 caggcaggtt attctgagtt agcttgattg gattatcaac ttctcctccg aggtgggtca
     4681 aggtctcccg cagggcaatt cgcaagaaag cgaaatgttc tacaccttct ttagaaaatt
     4741 gaacaaaaat caagtcattg gtcttgagat tttcagaaat gctaaactcc tctttccaga
     4801 gattagccag cgttactgat gtctccaaca aatcgtctgt aatatgattg aagaagggat
     4861 tttcttcttc gaaaatccca gtcttggctt catctgaata cacatgttca atttttttac
     4921 gcaggtattc ttcgattttt ggagtaatat tgagaaactt atctgctaag aacagttcgg
     4981 tatcatccgg actgaactgg tgaataatgg ctttcttaat ataaatgtcc ataaaagttt
     5041 tagtcctcgt atagtaggaa ggcatctgtc aattctttga ctgcacttct cacttcttct
     5101 aatacagcct cattttctga attcttaagg gttttaatga tgaattcagc cactttgcga
     5161 ctttcttctt caccaaatcc acgtgcagta atggctgctg ctccgatacg aatcccactt
     5221 gtcttgaatg gtgacaagct ttcgtaaggg attgagtttt tatttaaggt aatattgact
     5281 tcatccaaca agttttgagc aactttgccg ttttctacaa ctttagtcac atcaacaagg
     5341 aagagatggt tttcagttcc acctgaaata atacggaaat cagggtcttg caagaagaca
     5401 tctgccatag ccttgctgtt cttaattaca ttggcagcat attccttgaa ggctggatcc
     5461 aaaacttctt tgaaggaaac tgccttagcc gccacaacat gctctaaagg accgccctga
     5521 atacctggga aaatagctga attgattttt ttagcaagtt cttcgtcatt ggtcaaaatc
     5581 aaaccaccac gaggaccacg aagggttttg tgggtcgttg ttgttgtgat atgagcgtat
     5641 ggaactgggc ttggatgaag gccagccgca accaaaccag cgatatgggc catgtccacc
     5701 atgagcttcg ccccaacagc atctgcaatt tcacggaatt ttgaaaaatc gataatttga
     5761 gaataggctg aagcaccagc tacaatcagt tttggtttta cttcttgggc ttgtttcaag
     5821 atagcatcaa agtctaagag ttccgtttca ggatccacac tataagaaac aaagttgtag
     5881 gtttgaccag agaagctaac aggagcccca tgagtcaaat gaccacctga tgccaaatcc
     5941 attcccataa ccgtatcacc tggctcaatc aaggacatgt aagccgcaca gttagcttgg
     6001 cttcctgaat gtggttggac attggcaaat ttagcaccga aaatttcttt tgcgcgttca
     6061 atagcaagag tctctacaac gtctactaca tcagttccac cataataacg gcgtcctggg
     6121 taaccctcgg catatttatt tgtcaagata gacccttgag ctgccataac agccttggaa
     6181 actacgtttt ccgaagcaat taactcgata ttattttgtt ggcgttcttc ttctttggca
     6241 atagcattcc agagatcagc atcatatgct ttaaaatcat ctttgtcaaa aatcataggt
     6301 cttctccttt attgtgtgac tagtccatta gtttgatttt acaataagaa aatcaaacta
     6361 aaagatgcga ataaaccgtt tctgcatttt atcacaagta tagccaactt tttcataaaa
     6421 tgcatgagca cccagacgat gattggcaga atttaagcgg ataaacccat aaccacatct
     6481 tttggcttct tgttccaacc cttgtagtaa acttttaccg ataccttgac cttgcgcttg
     6541 aggtgaaact gctaaagcta agatattaaa tcctgctttg gaatagagtg attcgtaaac
     6601 ttcagcgtgg acatatccaa gtaagacatg attagctgca tcctcatagc caagtaggaa
     6661 atgatgggaa tcctgagaca gtctagctag ttggctagcc gtttcctctg gactaaaagt
     6721 ataacccaaa gtctcttggt tgatgtcaca tatcgctttc acatctgttt cttgcaaatc
     6781 tcttagcatt tcaagcctcc tcaaaagaaa tctctggcaa ccgagcaaga atatcttctc
     6841 gtttaattgc gccttggcgt aagattttca ccttgtctcc agacaaatcc acaatagttg
     6901 aatcctgtcc agttagaaaa gcatcgtctt ccagacccag aacctcttgg tcaaaatcct
     6961 tcagaatttg ttcaaaggtt acaccacttg cctgacctga gatattggca gacggcccaa
     7021 tcaagggacc tgtctctcga attaaatcca gtgtgatagg gtgactgggc atccgaaatc
     7081 caatagttgc aaggtcagaa tttacccaat agggaactcg gtcattggct tcgagaataa
     7141 tggtcaaggg acctggcaaa aaggtctcta caagtttttg tagataagct ggctgattct
     7201 tagaaaagtg caagatatcc tctaaagagg caacattgag attgagcgcc ttgtctctag
     7261 gacgacgttt gagctggtaa acatggtcaa ctgctttttc gtctaaggcc ttggcaaaaa
     7321 gaccataaac cgtctctgta ggcagaatga cagctccacc cttttccaac tcttgtctaa
     7381 tcctgtccat catcaaccac gaccatccta tcttgaccaa attggtcttt gagtgttcga
     7441 actttttttt caggaagatg ttttctaaaa agttcgggaa cactttgacc ttgcttgtat
     7501 ccaatttcaa ggtaaatctt accaccatcc ttgagatagt cttttgcatc ttccgcaatt
     7561 ctacagtaaa tagctaggcc atcctcgtct gcaaagagag ctagatgagg ctccgaatgc
     7621 aaaacattca agcctacctc tgactcatct tcacgagaga tatagggtgg attggataca
     7681 attatatcat atttttcaga aatttctgca aaacaatcag attttttaaa aaatatatta
     7741 agattttgat cgctagcatt ctctgatgca agctctaagg cctcttgaga aacatctgct
     7801 gctgtcactg accaatctgg tctatttttt gctagagcga gagcaatagc tccactacct
     7861 gttccaatat ccagaactga gagattcgtc tcaagatttt cagttagaat aagctccact
     7921 aactcctctg tttctggacg aggaatcaaa acccgttcat ccacctttaa atgcattcca
     7981 taaaaatctg cctgtccaat gatgtactga gctggcttgt gagctgctag ttgctggtaa
     8041 atatcttcta caaattgttt ttcttcctct gttgtcacct cctgctggag ggcaaaaata
     8101 aagtctgtaa aagatagatt tttcagacta cgatagacaa aagagaggct ttccgcttcc
     8161 tctccttgtc ttatcaactc ttcttcaaaa tttgaaaata attgagctaa tttcattatt
     8221 tgtttaattc ttctagtttt tgtgtttggt cataaagcac caaggcatcc acaacttcgt
     8281 ccaatttacc agacaaaatc gtatctagtt tttggagggt caagccgata cggtggtctg
     8341 tgacacggtt ttgtgggaag ttataagttc ggatccgttc tgaacggtca ccagtaccga
     8401 ttgtcgactt acgctcagcg tcttgttcat cctgagcaat ctgagcaaag tggtcagcga
     8461 cgcgtgcacg gataatcttc atagccttct cgcggttctt ctgctgggta cgttcttcct
     8521 gcatctcaac cttgatattg gttggcaagt gaacgatacg aacggcagtc gcaaccttat
     8581 tgacgttctg tccaccagca ccagaggcgt gatagatgtc gacacgaagg tcttttggat
     8641 caatgtcgta ttcaacctct tcaacttctg gcataacaag aactgtcgct gtcgaagtat
     8701 gaacacggcc ttggctttct gtcacaggaa cacgttgcac acggtgggca cctgattcat
     8761 acttaagctt agagtataca gactgacctg aaaccatagc aaccacttct ttaaaaccac
     8821 cgacaccatt catagaggct tccatgactt caaagcgcca accttgggct tccgcatact
     8881 tttggtacat agttagcaaa tctccagcga aaagtgccgc ttcgtctcca ccagctgctc
     8941 cacggatttc aaggatgata ttcttgtcat cgtttggatc ctttggaagg agcaaaattt
     9001 tcagtttttc ttcatattct tctttttcag ccttggcatc tttgagttct tgcttggcca
     9061 attcttccaa gtccgcatct ccgcctgatt ccttaatcat ctcttcggca tcgacgatat
     9121 tttgaaggac ttgtttatac tcacggtagg ctattacggt gtcacgattg gaagcttctt
     9181 cttttgaaag ctccataaaa cgcttggtgt ctgaaacgac atcagggtca ctcagcaatt
     9241 ctcctagttc ttcataacgg tcttctacag cttgtagttg atcatagatg ttcatttttc
     9301 ttcattctcc ttattgattt caggggcaaa ataatgttta cggcaaaccg agatataggt
     9361 ttcattacca ccaatttgga tctgttctcc atcgtaaacg ggcagtccat cctgtgttcg
     9421 caacaccatg gtcgcctttt tcttgcaata ctgacagatg gtcttgattt cgtcaatctt
     9481 gtctgctaaa agcaagagat atttggaacc ttcgaacaat tcattgcgaa agtcattttt
     9541 caagccaaaa gccatgacgg gtatgtctaa ctcgtccaca acacgagcta ggtcataaac
     9601 atggtggcgt ttgagaaact gggcttcatc gaccaacaca cagtaaggtt tttctggtag
     9661 gcctcggata tagccaaaga tatccgttgt ttcctcaatc gcaagggcag ggcgtttcat
     9721 gccaattcga ctcgacacat agccaacacc gtcacgcgta tccagagctg aagtcataat
     9781 cacaacacct tttccttgct cctcatagtt ataggccacc ttgagaatct cgattgtctt
     9841 tccagagttc atggtcccat aacgataata caactgtgcc atgtttcttg cttcacgtcc
     9901 atttctaaat ttttgctaca ttctagtata tcataatttt cttaagcttt aaacggcaaa
     9961 atgtggtaaa atggaagaaa tcaaaactag tggaggaagc tattatgcca tttgtacgca
    10021 tcgatttatt tgaaggacgc acgctcgagc aaaagaaagc tcttgctaag gaagtaacgg
    10081 aagcagttgt ccgcaacact ggagcccctc aatctgctgt ccatgtcatc atcaacgaca
    10141 tgccagaagg aacttacttc ccacaagggg aaatgcgtac taaataagct agcttaagca
    10201 gaattgctta ggctttttca atctccaagt agcattcatt gaagaaatat cctaaatttg
    10261 ttacaatttg aaaagaaact tggagaattt ccaagaaaat agctattaat taaaggaaac
    10321 attatgatta cacgtgaatt tgataccatc gctgctatct ctactccact aggtgaaggg
    10381 gctattggta ttgtccgcct gagcggaaca gacagttttg ctattgcgca aaagattttt
    10441 aaaggaaaag acttgaacaa ggttgccagc cacactctca actacggtca cattattgat
    10501 cctctgactg gtaaagtcat ggacgaggtt atggttgggg ctatgaagtc tccaaagacc
    10561 ttcactcgtg aggatattat cgagattaac acccacggtg gtattgcggt gaccaatgag
    10621 attctccagc tagctatccg tgaaggagtt cggttggcag aacctggtga atttaccaaa
    10681 cgtgcctttt taaacggtcg cgtagatttg acacaggccg aggcggtgat ggatatcatc
    10741 cgcgccaaga cagacaaggc catgaacatc gcggtcaaac aacttgatgg ttccctttct
    10801 gatcttatca ataatatccg tcaagaaatc cttaacacac ttgcccaagt tgaggtcaat
    10861 atcgactatc ctgagtatga cgatgttgag gaagccacta ctgctgttgt ccgagagaag
    10921 acaatggagt ttgagcaatt actaaccaag ctccttagga cagcacgtcg tggtaaaatc
    10981 cttcgtgaag gaatttcaac ggctatcatt ggacgtccca acgttgggaa atcaagcctt
    11041 ctcaacaacc tcttgcgtga ggacaaggct atcgtaaccg atatcgctgg gacaacacga
    11101 gatgtcatcg aagagtacgt caacatcaat ggtgtacctc tcaaattgat tgatacagct
    11161 ggtattcgtg aaacggatga tatagtggaa caaatcggtg ttgagcgttc gaaaaaagcc
    11221 ctcaaggaag ccaacttggt tctactagtg ctaaatgcca gtgaaccact gactgcgcaa
    11281 gacagacaac ttcttgaaat tagccaagat accaatcgca ttattctact taataaaacc
    11341 gacctgccag aaacgattga aacttcgaaa ctacctgaag acgttatccg tatttcagtc
    11401 cttaaaaacc aaaacatcga caagattgaa gagcgaatca acaacctctt ctttgaaaat
    11461 gctggcttga ttgagcaaga tgctacttac ttgtcaaacg cccgtcacat ttccctgatt
    11521 gaaaaagcag ttgaaagcct acaagccgtt aatcaaggtc ttgagctggg gatgccagtt
    11581 gatttgcttc aagttgactt gactcgtact tgggaaatcc tcggagaaat cactggggat
    11641 gctgctccag atgaactcat cacccaactc tttagccaat tctgtttagg aaaataagaa
    11701 aaatccatga tccttcattc ggtcatggat tttattgtct ttattagtaa tctggtctta
    11761 agacccctgt tacagttgcc ttagttgctt cgtagtcgcc atctacgaca accttgataa
    11821 tgcgtttggc atcttcttct ggtgctggaa caagaggtag acgagtgggt ccagcttcaa
    11881 atcccatata gttaagaatt gccttaactg gagcaggact tggataagag aagagagcat
    11941 taaccttagg aatgaattta cgctgaattg ctgcggcttt cttcatatcg ctttctgcaa
    12001 tggcagtaaa catctcgtgc atttcatccc catttgtatg agaggcaaca gaaataaccc
    12061 catccgcccc aaggttcatg gcatggaaag catctccatc ctcacctgta taaatcaaga
    12121 actcttcagg cttgtgctca atcaagtaag ccatattagc caagctagta cattctttga
    12181 caccgataat atttggatgg tcagccaagc gaagcatggt ttctggagtc aattcgacaa
    12241 ctacacgccc tggaatgtta tagataataa ttggtaggtc agaagcatct gcaatagtct
    12301 taaagtgctg atacatccct tcttgagaag gtttgttgta gtaaggaaca atagcaagcc
    12361 cagctgcgaa accaccaaat tccgctactt ctttgacaaa ctcaatagag tcacgcgtat
    12421 cattagtacc tacacccgca atcaaaggaa cgcgtccatt gacaaccttt tgtacagccg
    12481 caaacaactc caactcctca tcgtgggtca aagttggact ctcagcagtc gttcctgcga
    12541 gaagaattcc atccgtatga tgggccaata aatgctcaat caaggctgga atagcatcaa
    12601 agttaataga accatcctca tgaaaggggg taataaaggc tgtaatgatt ttacattctt
    12661 ttaaatcttg ataagacatg aaaacacctc tctatttcaa agaaggagtt catctgaact
    12721 cctaaacgat atgactattt taattcaaat ttcaattcag ctgttggacg aaccaatcca
    12781 cgttcatgaa gagtttcagc aatctgaact gagttccaag cagcaccttt gagaaggtta
    12841 tctgaaacaa cccacatgtg aattcctttt tctgcatcca agtctttacg gatacgacca
    12901 acaaaggtat cacgtgaacc aactgcattg atggcttgag gatagatttg atgaactaca
    12961 tcatcttcaa gaacagcacc tgggaaggat gcgatagctg ctttgacttc ttcaattgga
    13021 gccacttcct ttgtttcaat ataaactgac tcagagtgag ctgacaagac tggaatacgc
    13081 acacatgtcg cagatactgc aatgctatca tcttccataa ttttcttagt ttccttggtc
    13141 atcttcatct cttcgtacgt gtaatcatta tcagtgaaga catcaatttg tggaagagcg
    13201 ttaaaggcga taggataatg tttcttgtca ccacctgaag gcaagatttc cgcatgcaaa
    13261 tcacgtggtt tcactccatc attcaagacc tcacgaagtt cacgttgtgt ctcaagaatt
    13321 gctcccatac cagcacctga aacggcttga taagttgaaa caatgatacg gtccaagccc
    13381 catttttggc gaaccggctc aagagccacc atcatttgga ttgttgaaca gttagggcag
    13441 gcaatgattc cgttgtgggc atcaagtgca tgagcattga cctctggaac aaccaaagga
    13501 acatctggat tttgacggaa ataagatgta ttatccacta ctaccacgcc agcttttact
    13561 gcgtatggtg catacttagc tgatgtcgaa ccacctgctg aaaagagagc aatatcaact
    13621 ccttcaaaag ctgtttcagt cgtttcttca atcgtaatat cttgatcttt aaatttcaat
    13681 gacttacctg ctgaacgtgc agaagcaagg taacggattt tatcgattgg aagtgttgat
    13741 tcttccaaca tttttatcat ctgagcaccg acagcacctg tcgcgccgac tacagcaact
    13801 gtatatccca taaataacct ctttcggaat tttctaaaaa tttctataat agaagtatta
    13861 tactactttt tccatgaatt gcaaagcttt ttcatcattt tttggaaaat aaaaatcccc
    13921 aatcgctaga ctagggatta agaggcatct tcatgtgatg agcaggttca cacaactcat
    13981 caaggtccgc tcctgcgtta tgacctcctt atgctcaata gtagcccgaa gactacctat
    14041 cgactcatcg catctactat tctactaaag agaatgactt ttgtcaacag acgaaactct
    14101 ttatactctt cgaaaatctc ttcaaacttc gtcagcgtcg ccttgccgta tgtatggtta
    14161 ctgacttcgt cagtttcatc tacaacctca aaaacatgtt ttgagctgac ttcgtcagtt
    14221 ctatctacaa cctcaaagca gtgctttgag caacctgcgg ctagtttcct agtttactct
    14281 ttgattttca ttgagtatta gttttattta cacaggtgca taagaaatat attggaaaaa
    14341 ttgggggcag acataacaaa aacccttgta aatcaagggc ttttctgtct atttaatgct
    14401 aattgccggg attgaaccgg cgacctcatc cttaccatgg atgcgctctg ccaactgagc
    14461 taaatcagct tacttaagaa gtatactata atcagaaaaa cttgtcaagt ttccttagaa
    14521 attttccata ggaaaaagcc aggtaagaac tacctagctt atcttcttct atttacttaa
    14581 atcaattcga cgaccaattt taccaataat aatcgctcca ataatcaatg aagcaaattc
    14641 accgattcct gttgtaaacc aagtaaggaa gaatggtaat tctgctacaa tatttaactc
    14701 tgcagcgata gtaaacatgg aaattgaaaa gaggattgaa aagaagaaat gatcttttcg
    14761 aatcaatccg ttaaagaggt aatccttgct atattttgca aaaagccaaa cacctaaact
    14821 gaggaatact aaggttgaac caccaccaac aaagacatct agcagtccga agctaaagaa
    14881 attagcaatc atacaaccga tggtaactcc gatgatgtac ttaggattgt aaaaagccat
    14941 aaagttcatc atttctgaaa tacgaaactg ataagcacca tagctgatgg catttagggg
    15001 cggtgtgaca gtcaaaacga cgtagatagc agcgacgatt gcaatatctg caacatcacg
    15061 aatagttaat tttttcatct tttctccttt ggcggttatc cgcgtgtaat atgcttggtg
    15121 aaagaagcta agcaccaagg gttgattcat tcaaccttac tagtatagca caatagcctg
    15181 ctttatgcta tacttaaagc atgaaaaaac ctattcaaaa attttttaac aatgaaatct
    15241 tagcctatct cttttttggc cttgcgacaa ctctagtttc gattctctca cgactagtca
    15301 tttatcaact aagtcatcag gaactccttg ctacggcctt ggcaaatatt atcggtatct
    15361 tatttgcctt tatcacaaat gatacgattg tatttaagca aaaaaagaaa aatagactta
    15421 tccgtttagt gaaattttct cttgctcgcc tttctacttt tctgttagac ttgtttttaa
    15481 cttttctctt tgtaacccaa tttcctcaca tcatagggca attcgtaact gaaaacattg
    15541 ataagataaa tgctattgaa acaataattg cacaaatatt cattattatc atcaattata
    15601 tcttgagcaa ggtctatatt tttagaaaat aatgctccat ttatgagcct tttaattgcc
    15661 ttctataatg atttaggata caatgaaaca cgaaacattg aaaggaaaac agtatgttaa
    15721 aaaatctaaa atcgttcttg cttcgaggaa atgttattga ccttgctgtc ggtgttgtca
    15781 ttgcctctgc ttttggtgct atcgttactt cacttgtaaa cgacattatc actcctctta
    15841 ttttaaatcc cgctttgaaa gctgctaaag tcgaacgtat cgctcaactt tcttggcatg
    15901 gagtcggcta tggtaacttt ttaagtgcta ttatcaattt tatctttgtg ggtaccgccc
    15961 tcttctttat tatcaagggc attgaaaaag cacagaaact gactggcata aagaaagaaa
    16021 aaactgccga aaaaaaacca accgaattgg aagtccttca agaaataaaa gctctccttg
    16081 agaaaaaata atcgattaaa aggatctagc gcaaagctaa atcctttttt ctttactctt
    16141 tgggtaactt gcctgctttt tcaagcattt ttttgataag ataaggcatc ttaacattct
    16201 cccgcccttt tttctcaatc agttttttca caaattctgg catttgtaaa tcttcagatt
    16261 cagctaaaat gtttttcgtc tcgtctgaag gaactaactc atcaaaggtc tcttcttctg
    16321 ggagaaattc cttaaactct tgattttcat tgactctaac tgtattgacc aaagcatcaa
    16381 tcaaacgaga atctgtattt ggcattggtg gacgatggta gtttacccca aattcctggc
    16441 ataagtcata acattctaca tcattgtcaa acaagacctc aatatgctca ctgatgaaac
    16501 taataggtac aaaaatataa tggtctggat gttctgtctg ttctctgaga tactccaaaa
    16561 catctggttt aatccatgga ataccaatat cactctcact ctgccaagtg ttggtatatt
    16621 gttctgaact caagcccagt ttttcagcga ctagcttgct attttcgaaa atttggtcta
    16681 tataaggatc accaaagtcc aaggcaaaaa tgggcacact atgggctgaa aagatgactt
    16741 taaagctgtc ttgctttact tcttctttta aaatcttagc aatttcatct gcccaatagt
    16801 ttaagagcgc ttcttcctga taccagtcct taatgaccaa aaattgaatt tgtttgcttt
    16861 ctaaaaattt ttcatagccc atgacagagt agaaagaata atggggctcc aaaatcaagc
    16921 aaatacactg ttcaattccg tctgcttcca tttgaccgat cacatctgga ataaagggac
    16981 tggaaaactt attagcaaag tagacactgt attcattccc cagcctagct tctaccaagg
    17041 caacctcttg acgggtaatt ttttgcagag gcgtgcctcc aattcgtaca taattatcat
    17101 agagagtctg aatctcgtgg tcttggggtc tcaccccacg acgaatattt gtgaaaaaat
    17161 cagctacacc ttcaaaggta atctcttctg gtgaaccgaa ggtcatcatt aaaattgctt
    17221 ttttcatgac tgcttccttg tgatgttttt atcaagttta ttttatcatt aattcatcga
    17281 taagtaaacg ctaacatagg gacattctca atcttctgtc ttttgttttt ctcttctttc
    17341 tatgatacaa tggaaaaaat aaattcaaaa ggagtttttt tatgacttat ccaaatctct
    17401 tggaccgctt cttaacctac gttaaggtca acacgcgctc tgatgaacac tctactacta
    17461 ctccaagtac acagagtcag gttgacttcg caacaaatgt cctaattcct gaaatgaaac
    17521 gtgttggact gcaaaatgtt tactatctac cgaatggttt tgctattgga accttgccag
    17581 ccaacgatcc gtctttaaca cgtaagattg gttttatatc gcacatggat actgctgatt
    17641 ttaatgctga aggagtcaat ccacaggtaa ttgaaaacta cgatggtggt gtgattgaac
    17701 tagggaattc tggtttcaaa ctcgatccag ctgacttcaa gagtcttgaa aaatatccag
    17761 gacaaacgct catcacaaca gatggaacaa ccttgctagg tgctgatgac aagtcaggaa
    17821 ttgctgaaat tatgacagcc attgaatatc taactgctca tcctgaaatt aagcactgtg
    17881 agattcgtgt tggttttggt ccagatgaag aaatcggtgt tggtgccaat aaatttgatg
    17941 cagaagattt tgatgtggat tttgcctaca ctgttgatgg tggtccacta ggtgaacttc
    18001 agtacgagac tttctcagcc gctggtgctg aattgcattt ccaaggccgt aatgtccacc
    18061 ctggtactgc caaagggcag atggtcaatg cccttcagct agcaattgat tttcataatc
    18121 aacttccaga aaatgaccga cctgagttaa ctgaaggtta ccaaggtttt taccatctaa
    18181 tggatgtgac aggtagtgtt gaggaggcgc gtgcaagcta catcattcgt gattttgaaa
    18241 aagatgcctt tgaagcgcgt aaagcatcca tgcaatctat cgctgataag atgaatgaag
    18301 aacttgggag caaccgtgtc actctcaact tgacagacca gtactacaat atgaaagaag
    18361 tcattgaaaa agatatgact ccaattacca ttgctaaagc cgttatggaa gatctaggta
    18421 tcacgcctat tatcgaacca atccgaggtg gaacagacgg ctctaagatt tcctttatgg
    18481 gaatcccaac tccgaatatc tttgcaggtg gcgaaaatat gcacggacgt tttgaatacg
    18541 ttagccttca gactatggaa cgtgcagttg ataccatcat tggcatcgta cgtagcttat
    18601 aaaagctaaa aagacgaggt agctcagcta cttcgccttt ctttttattc tactggtttt
    18661 tcttgatttc cagtagttgt agaagattct gttgttttat tttctgaagt tgattcagca
    18721 ggtttagaat ctcttgtatt gcttggtttg ttttcgtcgc tagcagtttc aatgttagat
    18781 tctgcagttg cgtttggttg gttctcagca ctggtgttat caccatttgc ttcagcattt
    18841 cttgctggac ttgtttcttc acttgcgcta gcttttgact ggatttgatg attcaaaact
    18901 agaatagctt ttgtcgattc aagtaaagct gttttgtctt tactcttagc agaaagttga
    18961 tctaataatg catccacctt atcaaagtcc gcatcagatc cattattact ttctaaataa
    19021 gagtgaagcg acatgagaat atcgtagagt ttttgataga gtacaagtgt ctgaggatct
    19081 tgctcagcat tttccttttc ttgttgaagg gcgctagcga tacgagtcaa aacatctttt
    19141 acctgactgt ttacttcatc caagtctgca tcagccttgt ttgtggcagc ttttagattt
    19201 tctacttctt ctgccaagga ttgtctgatt ccttcttcat ggatttgttc caagagttga
    19261 tttgccttgt tcaaaagact ttctacttct tccttgctat ctgtcgcaga ttattggttg
    19321 ctatctacca tgtactccta aaacaggaga gttataatcc aagattacaa ggccttacag
    19381 aaataagaaa tccagataag acaatgttcg tccaagacgc tattcgcttc gcacagcagc
    19441 acggattcaa tatgctttaa ttttaaagtt taggtgtcaa gacctctttt tagtgtgccc
    19501 aaaatttaga gaagtaatca atcaactaac ttttattttt ttcaaacttt cagtaaactg
    19561 acctaaagct aactcaatct gtctttgtag atgcttctgc tatcagctag aagttgatct
    19621 acttttgcca agactgcctt ctcatcaaaa gttccaggtt gatagttgga ttgcagggat
    19681 ggaatcttgt ttttcaaagc cgcttcatat cccttagttt gaaccttgat gtagtgattg
    19741 tggtcgccat gaggaatcac aaaaccttct gaatcttcac ttataattcg attggcatca
    19801 aaaaccatga ccatcttctt cctcatggtg gacatgtagt gacggattac ttaatacaga
    19861 actagaagaa cttcctacct cttccgtgtt agagtgtgat gggagattgt taagagatga
    19921 cttaggaata tagtgatagt gttccccatg tcttactata taagcatcac ctatatctct
    19981 gacaatatca ttagggttaa aaacatatgt ggctgctaat tcacctgccg acaagtcact
    20041 ctcaggaatg aaatgatagt gaccaccatg tggtactata gtaggttgaa atagaatatg
    20101 agcaaattga taaggggatt ttaaagtaat ttctaacaat gatttagaaa ctatgatgtg
    20161 ctattctaaa ttcaactcac tatatataac catcatcggt agtataacgt ccctgtaatt
    20221 ttgctacaga tacttctgca ctagctcctt tatcgtcttt accatgttct tgtttttggc
    20281 gattgatttc atcttttgtt cgtacatttt ctgcatgagc ttaatcttta aggtaaacat
    20341 aatactttcc atctacctta atactatatc ctcccttaac ctaactgacg atatcttgat
    20401 ctttcggctg atagttgggg gctttcatta atggctcttc actaaagagc gcatcaaaag
    20461 gaactttacc attatagcag tgataatgat cgccatgaga agttacataa ccttgacctg
    20521 taatcttaat aacaatttgt tttgcttgaa ttccttcttt ttgactaacc tagtctggag
    20581 tcaaattttc agtcttctta gtgtctttat tactgtttac atatgaaaca cgatttttat
    20641 ctgtattggc ctgttagcta tgttggttca gagcataaac acacagactt aaggaaagga
    20701 taacaacaga tccagctgct atatatttct ttttaaattt cataattacc tcatttctat
    20761 aattatttat atgatgtctt cattattaaa cgattaaata aattaattaa ccaattaatt
    20821 aactagtaaa tattccacct ctttttaagt tgtatgtcaa gaaattttat atattaataa
    20881 taaaatgaaa ttctcccaaa gtcagagttt tatttctaac ttttgagaga acttcatttt
    20941 tgattcagac tttttctact gctattcctt acgctatgag atcagataaa ttctttttta
    21001 tcacttctcc acttggcaat cttaattcaa tcgttccatc catattgaat ataacactat
    21061 ctaagcctaa tccgtaacta gctgtaaatt tttctaattt ttcttgtaca ggatctactg
    21121 ctggagcttc ctctaatgct ggatctaaca tagggtcact ccccacattc ccttctggat
    21181 tcaacattcc attatccgtt gagttttctg gttttacagg tttttcgttt ggtgcctctg
    21241 gtaaagaatc tgctggttta ttttctgttg gttggttctc aactgttcca gtaggtactt
    21301 ttctattttc agatggttta ttttcaccat ttccttgagg tgcttctcct gtaaaatctg
    21361 ccatattctt tttaatgact tctcccgatg gtaaatataa ttcaattgtt ccgtccatat
    21421 taaacaagac attttctagc ttcatcccat aactttcagc aaattttgct actttttctt
    21481 gtacaggatc cactgtagga acttcttcta acgttgaatt actagtacta ttcccagttt
    21541 cagaaagttt ttctttttct accttctcac tagtctttgg ttcttctacc ttttcatcaa
    21601 gttttgagtt ttcttgtgct ttattccttt taaattgtgg tagaatactt ggtttatcag
    21661 tttgattttc tttttccaag ataggtactt ccacaatata agtcgattga ttgtccaaat
    21721 aagcatttgc catgaaggtt acaggaattt tatttccggc cgttctggtt gttccttggt
    21781 ttaatttcgg aatcggtaat ttgatttcac caactttata gttattttct aaataagcat
    21841 ttccatgaaa ttcatcaaac actctgacta aagcatcagt tcctttaggc actgcaaatt
    21901 gagggttcac tcttaaataa gtatcccctg catggaaagg atagaaaatc gtttgactgg
    21961 ccattttgta agctaaagag gttggaactg taaatgtacc atcataactt acttctggat
    22021 aatcttttga agcgatagta tacttaaatg tttgtcctgg taaataaggt tgatctaatt
    22081 caaagtttgc aatattccct actccttctc caaatacttt accagatact ttctccaata
    22141 cttttccatc tggtgttatt aattttacta gcatattgat acctaatttt ttctccaatt
    22201 caggcggaaa actaaaagaa acgcgttttt gaccattggc tagagtaaag ttttgattat
    22261 taaacgtact attttttaac aaattaacaa cattcgttaa ttcttctcca gtataaactt
    22321 tattcccttc ttttttagca actccttctt cgggtttaaa cagttcatag ttactgtgag
    22381 aatgaccaat tccaaccggt ttatgttcat caatcggatc tgcatgatgg tgatctccat
    22441 gcggataaat aatcgcattt ttttctttat tcacgacaat actttcacgt ttgacaccat
    22501 attgtttcat aatgccagca attttttctt cgattttttt atctaaatct ttcatttctt
    22561 tggcattact tggataatcc tgttcatgag atgacaaaga atctaatcca ttatgactag
    22621 ttttaacttc ctctaaatgt ttttgcgcag ccttaatttg ctcttctgtc aagtccttct
    22681 tgaagaaata atgattgtgg tctccgtgac tcatgacaaa acctgattca tcttcagcga
    22741 taatacgatt agcatcaaat ccgtatccat cttcttcatg tttctcatgt gaagttcctg
    22801 gattgattgg aagagatgga gaaggtgttg ctagactatt gtttggaaga gtcggttgcc
    22861 caatttgatt tgattttgga atgtaatgga aatgatcacc atgtcttaca atataagctg
    22921 tagccgtttc ttcaacgata tcttttggat taaaaatata accatcagat gctgaagaga
    22981 gctccttact tgtcgttaaa gaagaaggat tgcttgaaag actgcctaga ctagacacta
    23041 cttcattagg ttttgcattt gtagaaactg tagaaccagt tccactgata ggcaccattc
    23101 tggcaatctt ttcttctaag gcagaaagct tgctgtaagg aataaagtgg taatggtcgc
    23161 catgcggaat cgcaactcca tttggtgtac gactgataat cttagcaggg tcaaagacca
    23221 ggccatctga ttcactgtaa cgttgggcgc taggtgaatc atagagttcc ttcaaaagac
    23281 tctggagatt ttcagattta tttgctggct tgctagttga tccttttgct acagattgcg
    23341 tgttattgtc actagctgtt gaagaatagc ttaactgact cggttgcata ttttttccag
    23401 ccagatgtgc tttagctgct gctaattcac tagcagataa atcgcttttg ggaatgtagt
    23461 gatagtgacc tccatgagga acgatataag cattacccgt atcttcgata atatcagctg
    23521 gattaaagac ataaccatca tttgtcgtat atcgtccctg agaccttgct acagcaacat
    23581 tagagttaac cttctcatta tctttgacat gttcttgttt ttgacgattg atttcatctt
    23641 tagttcgaac attatcagca tgagctgcat ctttcaggta gacataatat tttccatcga
    23701 ccttgatgat ataaccaccc ttgacttcat tgacaatatc agcgtcttta agttgatagt
    23761 ttggatcctt catcaagagt tcttcactaa agagggcatc ataaggaact ttcccattat
    23821 agtaatgata gtggtcaccg tgtgacgtta catagccctg atctgtaatt ttgattacaa
    23881 tttgctcggc ctgaattcct tctttctggc taacctggtc tggtgtcaag ttttcacttt
    23941 tctgacttga ctggctgcca tctacataag agacacgatt attgtcctta ttttcctgcg
    24001 aacgatgctg gtttagtgca taggcacata gactcaagga tacgataaca gctgatccag
    24061 ctgctatata ttttttactg aatttcataa atccctcatt tcaataaatg atgaagtttt
    24121 ttctcaactt cttttacttt attaaatagt tttctaaacc ggttatttaa ccatttaatt
    24181 aaccagtaaa tagtttacta tttaaaatta cttttgtcaa gtttctgttg cgtttttcta
    24241 aaaaagagaa gtaacacgaa aggttacttc tccataaaat atgctccatt cattttacta
    24301 ctgtatagga gtcggttgac tttcttttaa caaagccaag agactatcta cttctgctga
    24361 aatagtgtta ttatctttcg ttccgagaag aagactactt tttagaccag tcaatgtctc
    24421 cacagcattt tgtctaatac tagaatctgt tactttttct agcaaggcct ccgcctctgc
    24481 tatcttagcg ttaataacag aatgctctac ttgaggaatt tcagcctcat ctgtagtatc
    24541 ttcagcttct tcctctgtct cttccgtatc agtgcttggt ttataaagat tatctgctga
    24601 aggatttaaa ccagcgtgat tctctttttc atcagattca gggtgagttg gctcacttac
    24661 ttcatcatgt cccttatctt catcaggttt actatcttgg tctgccttat ttttacgaac
    24721 atggtcgcta gcgttaccaa aaccattatc tgaatgcgga cgttcgtttg gatgttcgac
    24781 atagtacttg acagtcgcca aaagatcctc aagactatac cccttaggtg cctcataaag
    24841 gccttcgtca aaccactcaa atttgatgtt atggtaatgg tcataatgag gtatgattaa
    24901 actaccgttt ttgacttcta cagtatattg aagattgtaa ggcatacgat caagtggcac
    24961 cttcttagct gctttcacgc ggttgtagat agcttctgct ccttttgcct cagtatttcc
    25021 tgaatcctga tgg
//
LOCUS       DAVKQL010000030        24340 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVKQL010000030 DAVKQL010000000
VERSION     DAVKQL010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24340
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.030"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            70..267
                     /locus_tag="VW561_001165"
     CDS             70..267
                     /locus_tag="VW561_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU9943432.1"
                     /translation="MAGKITKKSSSMGIIANVFAGLVGASAGQSLLGSWGSSIAGMAL
                     LPSIVGAAIVITVVSFFTGRK"
     gene            351..917
                     /gene="amaP"
                     /locus_tag="VW561_001166"
     CDS             351..917
                     /gene="amaP"
                     /locus_tag="VW561_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEU9943433.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            929..1099
                     /locus_tag="VW561_001167"
     CDS             929..1099
                     /locus_tag="VW561_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEU9943434.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            1138..1746
                     /locus_tag="VW561_001168"
     CDS             1138..1746
                     /locus_tag="VW561_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9943435.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            1777..1980
                     /locus_tag="VW561_001169"
     CDS             1777..1980
                     /locus_tag="VW561_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU9943436.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(2075..2347)
                     /locus_tag="VW561_001170"
                     /pseudo
     CDS             complement(2075..2347)
                     /locus_tag="VW561_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2563..2838
                     /locus_tag="VW561_001171"
     CDS             2563..2838
                     /locus_tag="VW561_001171"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9943437.1"
                     /translation="MKTKSRKAKDMAESIQGWLAQFLVNLFKSITFDCGKEFSKWKDI
                     SNHHDSESFFANLGCPRQRCLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            complement(3116..3886)
                     /locus_tag="VW561_001172"
     CDS             complement(3116..3886)
                     /locus_tag="VW561_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU9943438.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPIPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(3901..4749)
                     /locus_tag="VW561_001173"
     CDS             complement(3901..4749)
                     /locus_tag="VW561_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEU9943439.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIADTPV
                     TNQEFVQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVSNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(4746..6221)
                     /locus_tag="VW561_001174"
     CDS             complement(4746..6221)
                     /locus_tag="VW561_001174"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEU9943440.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYASDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            complement(6544..7899)
                     /locus_tag="VW561_001175"
     CDS             complement(6544..7899)
                     /locus_tag="VW561_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9943441.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     NVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMAYKTEL
                     S"
     gene            complement(7909..8226)
                     /locus_tag="VW561_001176"
     CDS             complement(7909..8226)
                     /locus_tag="VW561_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9943442.1"
                     /translation="MNESNLESVMGLIMYGGEAKSNAMEAIQAAKKGDFSKANKKLAD
                     ANAALLKAHMAQTEMLTKEAQGEETSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(8243..8560)
                     /locus_tag="VW561_001177"
     CDS             complement(8243..8560)
                     /locus_tag="VW561_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9943443.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(8575..9471)
                     /locus_tag="VW561_001178"
     CDS             complement(8575..9471)
                     /locus_tag="VW561_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEU9943444.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLKRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(9474..10736)
                     /locus_tag="VW561_001179"
     CDS             complement(9474..10736)
                     /locus_tag="VW561_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9943445.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFIRGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDILLYLPFVIAIEKRFKLLED"
     gene            11016..12194
                     /locus_tag="VW561_001180"
     CDS             11016..12194
                     /locus_tag="VW561_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU9943446.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQSKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDDYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
     gene            complement(12422..12868)
                     /locus_tag="VW561_001181"
     CDS             complement(12422..12868)
                     /locus_tag="VW561_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9943447.1"
                     /translation="MTIRFEEKVSIENAQLVCQWSNSLGKVFQEQWMGPRIPFPLTLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQGQGLDSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVRKYSELKLE"
     gene            12938..13597
                     /locus_tag="VW561_001182"
     CDS             12938..13597
                     /locus_tag="VW561_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEU9943448.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTPLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELGLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTFHLILIASSGWNLVMVSFLILGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERFPFVPFLLLATCLIIFGKLLL
                     V"
     gene            complement(13649..14800)
                     /locus_tag="VW561_001183"
     CDS             complement(13649..14800)
                     /locus_tag="VW561_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEU9943449.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNIIIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(14815..15546)
                     /locus_tag="VW561_001184"
     CDS             complement(14815..15546)
                     /locus_tag="VW561_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943450.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
     gene            15775..15876
                     /locus_tag="VW561_001185"
                     /pseudo
     CDS             15775..15876
                     /locus_tag="VW561_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            complement(15848..16624)
                     /locus_tag="VW561_001186"
     CDS             complement(15848..16624)
                     /locus_tag="VW561_001186"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEU9943451.1"
                     /translation="MPKTLTEKLNAIKATGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFIYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIRQAVAYQK"
     gene            complement(16617..17840)
                     /gene="trpB"
                     /locus_tag="VW561_001187"
     CDS             complement(16617..17840)
                     /gene="trpB"
                     /locus_tag="VW561_001187"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU9943452.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGLGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            complement(17818..18417)
                     /locus_tag="VW561_001188"
     CDS             complement(17818..18417)
                     /locus_tag="VW561_001188"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEU9943453.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVDLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQSFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            complement(18404..19171)
                     /gene="trpC"
                     /locus_tag="VW561_001189"
     CDS             complement(18404..19171)
                     /gene="trpC"
                     /locus_tag="VW561_001189"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEU9943454.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDC
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            complement(19168..20172)
                     /gene="trpD"
                     /locus_tag="VW561_001190"
     CDS             complement(19168..20172)
                     /gene="trpD"
                     /locus_tag="VW561_001190"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU9943455.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            complement(20183..20749)
                     /locus_tag="VW561_001191"
     CDS             complement(20183..20749)
                     /locus_tag="VW561_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEU9943456.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            complement(20746..22107)
                     /gene="trpE"
                     /locus_tag="VW561_001192"
     CDS             complement(20746..22107)
                     /gene="trpE"
                     /locus_tag="VW561_001192"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEU9943457.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYIVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMEVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            complement(22608..22685)
                     /locus_tag="VW561_001193"
                     /pseudo
     CDS             complement(22608..22685)
                     /locus_tag="VW561_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(23292..24293)
                     /locus_tag="VW561_001194"
     CDS             complement(23292..24293)
                     /locus_tag="VW561_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9943458.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTSQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
BASE COUNT         7639 a         5197 c         3990 g         7514 t
ORIGIN      
        1 tgatattaaa ataaaaaagg agggtaggca gtgttgtgat caattattgc tggaggtctt
       61 attggtctct tggcaggtaa aatcactaaa aaaagtagtt ctatgggaat catcgcaaat
      121 gtattcgctg gtttagtcgg ggcatctgca ggacaatctc ttttaggtag ttggggttca
      181 tcaatcgctg gaatggcttt gctcccatct attgtaggtg cagcgattgt gattactgta
      241 gtgtcattct ttacaggtag aaagtaaact tttcgccagt aaagttagca aactattttt
      301 aaatcaatga cgggaaaaat agtttaaatg ttaaatcgaa aggattgtat atgtcaaaag
      361 caaagaaaat atgtttcatt attttctgta ttttaatctt gacaattttc cttcctgttt
      421 tgatagatta tcatcaagtt agtgatctag gtattcatct acttagctgg agacagaact
      481 ccgtagttga attctatctt gctagatatg tcttttgggg gacagtggtt ctatcaactt
      541 tagttttatt atccatttta gttgtgatgt tttatcctaa acgttacttg gaaatccaac
      601 ttgaaactaa aaacgataca ttaaaattaa agaattcggc aatcgaaggt tttgttagaa
      661 gtttggtgag tgatcataga ttgatcaaga acccaactgt tcatgtaaat ttacgaaaaa
      721 ataaatgttt cgttcatgta gaaggtaaaa ttcttccttc agacaacatc gctgacagat
      781 gccaaataat tcaaaatgaa ataactaatg gattgaagca gttttttggt attgagcgtc
      841 aagtaaaact tgaagttgca gtaaaaaatt accaaccaaa acctcaaaac aaaaagactg
      901 ttagtcgtgt gaagtaagga agtaaaaaat ggaatggctt aaacaatatc gatatccaat
      961 tatcgctggt ctcataggcg tatttctggc ttgtttgatt gtctcctttg gcttcttcaa
     1021 aacaatattt gtattgattt taggagcact gggagttgca gctggattat atatcgaaaa
     1081 aaactatata gataaataaa aaaataaaaa ttactaattt aattaaagga gtttcatatg
     1141 tcaaacgaaa aaaacacaaa cactaacgta gaaaagaaag atgctactgt tgtagctcac
     1201 gaaatcaaag gggaacttac ttacgaagat aaagttatcc aaaaaatcat tggtctttca
     1261 ctagaaaacg tttcaggtct tttgggaatc gatggtggtt tcttctcaaa tcttaaagaa
     1321 aaaatcgtta acagcgatga cgtaacaagt ggtgttaacg tagaagttgg taaaacacaa
     1381 gttgcagttg acttaaacgt tattgttgag taccaaaaaa atgttccagc tttatattca
     1441 gaaatcagag aaatcgtatc ttcagaagtt gctaaaatga ctgacttgga aattgttgaa
     1501 atcaacgtaa acgttgtcga catcaaaact aaagaacagc atgaagcaga ctcagtaagc
     1561 cttcaagatc gcgtatctga cgttgctgaa tcaacaggag aattcacttc agaacaattc
     1621 gaaaaagcta aatctggtct tggatctggt ttctcaactg ttcaagaaaa agttagcgaa
     1681 ggtgtagaag ctgttaaagg tgcagcaaat ggtgtagtat ctcacgaaaa cactcgtgta
     1741 aactaagata aaataaatat aacaggagaa attatcatgt cagtagaaga aaaattaaat
     1801 caagctaaag gttctattaa agaaggtgtt gggaaagcca tcggtgatga aaaaatggaa
     1861 aaagaaggag cggctgaaaa agttgtttct aaagtaaaag aagtcgccga agatgccaaa
     1921 gacgctgtag aaggtgctgt agaaggtgtt aaaaacatgt tgagtggcga cgataaataa
     1981 ggttaaaagt tactttatct ttttggtaac attagtcaag atgattctca gaaaacaaaa
     2041 agctagagat tcccaattgc ggaactctag ctttttaatt ttgcctcttt ctcttattat
     2101 atttcagcag gttgttggcc atgagtatga atcccatgtc aattctcact tgacgcttcc
     2161 ctctcagatg acatctctta taacccaaac aaacctttat ctgcccaaag acagatttca
     2221 tatcaatctt acgtttagcg aaaatttgtc tacccttgga agataaaagt gcctgatatt
     2281 ctttagtttt taaacactgg taacgttcat tcatatacag tctcttttga ggggctgatt
     2341 caggttcata atcgcagtca acattgattt caaggctgtt tgctttctat ctcccctttt
     2401 tcatgatata ttttcgtaca ggtgcttcaa tagtttctat agtaaaatga accaaaaata
     2461 gtacacgttg cggtataatg ttcttgtggc atattcatta gattttcgta aaaagttctc
     2521 tcctatttgt gagcgaacag gtatagtcaa tcgattgtca gtttgaagac caagagtcgt
     2581 aaggcaaagg atatggcaga atccatccaa gggtggctgg cacaatttct agtgaacctc
     2641 tttaaatcta ttacgtttga ctgtggaaaa gagttttcaa aatggaaaga tatttccaat
     2701 catcacgata gtgagagctt ttttgcgaat ctaggatgtc caagacagcg gtgtctcaac
     2761 gaacattcaa atagactttt aaggtgtcat gacttgccaa agcagacaga tttcaacgaa
     2821 gtcagtcaag aattttaggc tgggtaaaaa ctcaatttca ggagaaaatg aaataaatat
     2881 tcccacaata aaacgcatag tatcaaagtt tttccaacac ctgatattat acatttttta
     2941 attttaaaga ctttttccca gcctcaagaa atcacatccc acctaaatcc tttggatacc
     3001 aaatacctca tcaggtattt tctgagttgg taagactgtc tagcttaatt tgacaaatgg
     3061 agggcgttaa atccagtttt tacaggaagt ctatttaaat attttcacac tgaccttaca
     3121 aaatatgaaa taaattatat aaagtcattc ccatgacaaa gaataacatc cctttaaaac
     3181 agtatcttac ccaattttca ggtaagactt ttgatacaac cgtccccaat acccctccgc
     3241 atatagaagc aagaaaaata actggaatag gtgctaaatg atactgattt aaacctgtaa
     3301 ccacaacaat tgtagccaaa tgagacaact gggaaaagaa aataattgca agagaataca
     3361 tcgttgcttc ttttatcgaa atagaaaaaa acaccatcaa taaagataca ttgagcggac
     3421 caccaccaat ccctaaaaaa ctagaaatca agcccaatag aagaccacaa atcatataga
     3481 atcctatctt atcaaattga taagtttttt taaaatctgt taaaacaaat gatacaagta
     3541 acatcacaaa tagtaaaatc atttgaacaa tagatactag ccgtactgat aattgagata
     3601 acaaaacttg aaaaataaga tgacctagaa accctcctaa aacagaagaa aaggataccg
     3661 taaaaatcaa tctccatttt atctctttac tttgtgaaaa tcttttgatt gttgaaataa
     3721 tcgccattat aaaaacagag aaactagaat aaaaagcaat atcactgacc gaatggtatc
     3781 caaaactatc cataagtggc ttaattataa ttccaccacc catgcctgat atagaaccca
     3841 atccactagc tattataatg attagaaaat aaataatctc aaccactttt taagtccatc
     3901 ctattctttc aaacatctaa aacctagatg gctactagtg gaagtagaaa tacatccatt
     3961 tctagcagct actcggtatc gattacaata agaacaatga cataaaaagg aacctcctct
     4021 gatagcatac tcctcatctt gtatcccata ttttggtaac tcatagtcat cttcattaaa
     4081 actagctaat aaggtatatc tcggattgcg acaccattcc caaacattcc caatcatttg
     4141 atatagacca aaatcattag gtaaaaattc atagacagga gccgttccta taaatccatc
     4201 taaggcagta ttttcataag gaaatcttcc ttgccaagta ttggcatgat aatatccacc
     4261 ttctaaaaga gtatctcccc aaggatattc agattgtctt cctcctctag cagcatattc
     4321 ccactgtgct tcagtcggta aagacattcc gctccaatta cagaaagcca atgcatcttc
     4381 taaagcaaca tgaactactg gatggtcttc taaacccact agattactgt tctcaccgta
     4441 gggatgtttc cagcaagcgt tcgaaacttg taaccaccac ggagccccag caggatgagg
     4501 atatccctcc ctttctgcct ccggaacgaa caatataaaa acaaaagacc actcttgtcg
     4561 ctcagctagt gttttatagc ctgtttcttt cacaaattga acaaattctt gattcgtaac
     4621 tggagtatcc gcaatggaaa aatcctttac actaacagcg acttgaggac cttcaaaatc
     4681 atctaaaaag ccctcttcag aatttgttcc catcataaat gtcccaccct tgattttttt
     4741 cattttcatg attgactatc ccccgttttc gttagagttg gcgttatttt agaaagttca
     4801 acaggaacta actcttcatc cacaacaaaa ccttcttctc tatatctaag aaagtctact
     4861 aattttttct tcatctgacg aacaataggt tggtattttt cacttggata caaatcattc
     4921 atttcatgcg gatctttttt catatcaaat aactgataat gatttaaaac aggaaaccag
     4981 ataaatttcc actggtctgt caagatatat tgactagaat cttttcctaa agcatgttca
     5041 ccatgaaatt ctgttcgcca cccctcgtac tgtccaaata ataaattctt aacactccgt
     5101 ccatctagtt catctgtagt agttcctcct gccaaatcca cgagtgaagg aaaaatatct
     5161 tgaattttca ctaattgctt gattgttccc ctattaccag ctatcaatcc agccggatcg
     5221 taaataaacg aaggaatgtg gatacttccc tgataaggat atccctttct aaacagataa
     5281 tgttcaccaa gctgatctcc atgatccgaa acaaaccaga taatcgtatc tttatcatga
     5341 cgaaattctt ttagggcagt caaaaagcga ctaatttgat ggtctatatg agtaatcaat
     5401 ccaaaataag cagcaaccat tcttctttga tcatcttcct ttaatttccc tcttaaagca
     5461 taaatagacg gaatttgctt ttctaatact tcccaattcc cgatatgcaa atcgagaaat
     5521 tgaggcaagc gttccatata catatcaaaa tagtattttg ggggatttaa tggagcatgt
     5581 ggtttttcaa aagacatttt gagaaaaaaa ggaaccgttg gatctcttct ttgtaaaaat
     5641 gaaatggact cactcacaac ccagtttgtt ggatgtaact tctcatcttt atcccaaggt
     5701 ctagcttccc aagaattaca atccattccg tcatcaatca aatcaacatc ataaccaact
     5761 ttacccttta aaaaggctag atagtcacta gcataatcaa attgacttcc ataagcctta
     5821 tcatactttc tatcaacatg tagataccca tcgtgaagta agacatgatc aaacccaagt
     5881 ctctgtctgg aaggaaaaac atgcatctta ccaatacact ctgtttgata acccatatct
     5941 ttaaagactt ttggaagagt attagtaaaa ttccaaggca cctcatcttg ataaccaact
     6001 cgtccacttt tatcttggtc taagccagtc aataaagctg ccctagcagg aacgcaagaa
     6061 ggaactggag aataggcatt ttcaaaatta taacctacac tagccatcat atctaatgtt
     6121 ggagtgctaa ctagcttatc cttactattc aaggataagg cgtctgctct catttgatct
     6181 acaacaatca aaataatatt tggctgtttt gtctgaacca taaaatctcc tttctaatat
     6241 ggcaaaagag gcacaagaag atatctacct ttactgcacc cctttctata tcaatctctc
     6301 tatataaaac aataacattc ttgttatgtt ttatagaaaa atgaactaaa atatgactaa
     6361 atcgattagg aaattcaaat cattttctag tactgtttta gaagttacag tgtactattc
     6421 caacttcaat aaattataaa cctttgtcta ataacaattt tagtggagat aagaaatcct
     6481 acacctaact catcttacac gtaatctatt tctattttat cacaaaaaac gcaagtaaga
     6541 ccattaactc aattcagttt tatatgccat tttcacaaat gggaaataag tcaagacact
     6601 aataatcaaa caaacaactg ataagatgat ggcacgccaa tcaaatgctg tagagaagaa
     6661 accatataaa attggaggca ttacccaagt aacgttttgt gtaacaggtg aaacaagacc
     6721 ccagcttgtt gcccagtaag ctaccgttgc catgaaaacc gggctaagta caaatggtat
     6781 aaatagcaaa ggattcaaga caactggtaa accataattg accggctcac caatattaaa
     6841 cagaactggt gctagaccaa gtttagcaac ttttcgataa tgactgtttc ttgaaaaaat
     6901 taaaatagca agtactaatc ctaatcctcc aaaccagaca aacgccccaa aagacccact
     6961 tgtccatata taaggaatcg gttcaccttt ttggaaagca tccagattcg ctaacatagc
     7021 aactccaaac agcccttcca tgatgggagc caatacattt cctccatgga gaccaaaaaa
     7081 ccagaataac ttattcaaaa agatcatcag aataactgca aagaaacttt gagacaaacc
     7141 tagtaatggc gtttgtaaca ccttgtaaac ccaatcaatc aataagtcat tgctaagtaa
     7201 atggaaaaca taagtcaaga tggctactat atacatcgcc ataaatcctg gaatgataga
     7261 agtgaacggc ttagcaatcg cagggggaac tgaatctggt aacttgatta cccagttctt
     7321 tttcattact ttacagaaaa taatagaggc taaaaatcca atcatcatgg ctgtaaagta
     7381 gcctctggca ttaatatggt ttcctggaat cacattccca atagttacca tcagattttt
     7441 accatcaaat gctagattat caattccttg taagatttga tctaatttca catctcctac
     7501 atttgccaaa gggaaactct ttgtaactgt acttccaatc gaaatgacaa acgaagcaag
     7561 cgataccaaa ccagcagaaa ctgtatcaac attgtaaatc ttagcgatat tcactcccaa
     7621 gcaatagatg aacaacaagg aaacaattgg tatacttccc ttgaatacca aattattgat
     7681 gtcaacaagc cactgaaagg ttttcgtaat actttctagg tgaaattgtt gtggtaaatc
     7741 cactagaaaa gcatttaata acaaagcaat ggaacctgtc ataataacag gcatagtccc
     7801 cacaaatgaa tcacgtaaag caactaaaaa cttctgattc ccaactttca ttgcaattgg
     7861 agaaagtttt tcttgcattt tatccaacat tgtattcata ccaaactcct aatttttttc
     7921 aaatcgttcg tatacttcta ccacttcttt cgccaagtca acaaaggtca gactcgtcat
     7981 taaatgatct tgcgcgtgta ccatcaagag gctaattgaa gtttcttccc cctgtgcctc
     8041 ttttgtcaac atttctgttt gagccatatg cgcctttaat aaggcagcat tcgcatcagc
     8101 taatttttta ttggctttag agaaatcccc tttttttgct gcctgaatag cctccatagc
     8161 attacttttg gcttccccac catacataat tagccccatt acagattcta aattactttc
     8221 attcatagca ttctcctcaa tcttagtcta ataaactttc agctaaatct aagactttag
     8281 aaccattcat cattccgtaa tctgtcatcg gaataaccgc cacaggaatc tgtctatctt
     8341 ttagtttttc ttgaaagtcc cctagtaaat agcgaacttg agggcctaaa agcaacacat
     8401 ttacttcttt tgttgctaca atttcttctg cttcaggagc tggaactgca aaaatttctg
     8461 catccaaccc cttatcttct gctgcctttt gcatctttgt cactagcaga cttgtactca
     8521 tacctgctgc acatgctaac ataattgtta ctttagccat agcttactcc tttatcacct
     8581 atttactgac ctagccaagc gagatactgt atgtctcact cctctaattg ccttgctcaa
     8641 tgcataaata ttatcgactg ttgccagtcc cccatatcct gcgtcaccta tatgttgaat
     8701 atcaactcca caaattttat ttctaagtgc aatttccttg atagtatctg tatcagatgt
     8761 ttcttggcta gtaccaatag cactcaatac aagaccaccc ttactatgaa cgagatcaac
     8821 gacttcacgc aactcttggt catgaaaggc tggaacggtt ccaactgctg gaacaagtat
     8881 cacatccgca cctgcttcca acaattgctc tgcaacagaa agctctgcca caggctcatt
     8941 cactcctgcc ccgtgcatct tacctgcaat aatcaaacca gaaaagtttt ccttagcagt
     9001 ttgaacagcc ttaatgattt ctcgattgct aactcctgtt ccaggatttc cagtcaaaca
     9061 gacaaagtca aaacctaact cctctattcg cttcaatgtt tcaacactag caacacgacc
     9121 tgcaacaatt tcctgtgttt cctctagcat ctttgcagat gggtcaatcg gttccaaatt
     9181 tacaccaatt ggacaagcaa caagctggtg taagcgatga ataacatcat cacctgaact
     9241 atccaaagca taaattttag gctcaaaaac atcaacacaa ttcaataaaa tcatatcagc
     9301 tccaaaagct ctagcaattt cagaattagt tatatcccca acaaatgtct cacgagtaac
     9361 tacattttca gatagaacaa ctctaccttc acttgctaaa atactttgtt ttaattcttc
     9421 agcattcatc tgtaatatct cagatggatt tgcactaatt aaacgtttca ttctcaatcc
     9481 tccaataact taaatcgttt ttcaatagct attacaaatg gtaaataaag taagatatcc
     9541 aaaacaatca atacaatttg taataatgaa ccagaaatac ttcctgtagc aagtaaacca
     9601 cttataacag gtggcatagt ccaacctgga tctgtataag ttagcggaac caaacctgat
     9661 gccattgctc cccacgctac tagtaagtta aacattggag ctaagataaa tggaactagt
     9721 aacaaaatgt tcaaaacaat aggaacgcca aacattgctg gttcatttat attaaataat
     9781 ccaggtataa cagttatagg tgccaaagtt tttagttgtt tactagcttt cttcttatgt
     9841 gctaaatatc ctaacgcaag aactaaacct atggttgcac caccgccacc tatgaataca
     9901 aaattatcca taaatacgct agtaaaaata tttggaagag tttctccagt ttgataagct
     9961 actttatttg catctagatt agctaaccat aacggtttca taactgcatt aacaacattt
    10021 cctccgtgta atcctacaaa ccaaaaacta ctattaagga aaattaagat aagaagacca
    10081 ataacattat ttcctaaaag tccaagtgga cctcccaaag ctacttgagc aatttcatga
    10141 agattaggta aacttaattt atctaaagtt gcataaacaa tcaaccaacc aacaatagtc
    10201 actgcaccag gaataatggc actaaagctt ttagatactg ctggtggaac accatccggc
    10261 atttttatct gtatattatg atttataaac cattggtaga tatagccatt aatcaatcct
    10321 aaaatcatag caacaaataa accttttgat gccatgaaag cggtaggcat tcctgctcca
    10381 gcctctcctc taatgaaagg cgtaactgta ataaaagatg ataatgacac aataccagca
    10441 ggaataccat caactttgta ttgtctagcc ataaagtaag cgataccaaa actagctaca
    10501 agaccaagta atccaaaact actatcaaca cccttccata aatagtcagc aacaccaata
    10561 tcacccagat atttttccca tcctggaata ggaaaacttg caataaccat tagtaatgaa
    10621 cctatcaaca ataaaggtat agttagagta ataccgtctc gaatagaaac caacgcttta
    10681 ttattaccca atctagccgc tactggaagt aacttttctt gtaataattt ttccataacc
    10741 ttttcctctt aaatatattt tattatcaaa agttatcaat tatctttatt ttttgacgtc
    10801 aagttgttat ttgattatct tttgttattt atattttaca actataagaa agccttgtca
    10861 aggatttgtg taaatatttg attatttttt attatcaata tataataaaa agagaccagt
    10921 aagtaatgaa aagcttaaaa agattaacat ctacatttat ccatagcaaa aaatcatagg
    10981 attttaagaa tctgacaact aggaagaaaa ctaatatgac cttatctaag aaacaattac
    11041 aattaagagc taaaatactg gaaacagttt acacattagg tcccatttca cgaattgaaa
    11101 tcgctactaa aacaggaata acacctgcaa caactagcag tatcacaaat gatctgatta
    11161 aggaaaatat tctcttagaa cttggtgaag acgaacacga tacaagtgta ggaagaaaaa
    11221 aaatcctgct agatattcaa tctaaacgat tttactacat tggttgtgaa ctatctgaaa
    11281 aacatttcac ttttgcttta ggagataatc taggaaatat cttaaaggaa gagaaagaaa
    11341 ttgttactaa acagttaatc caagaaaagg gaaatcaact cattaaccag acactaaagc
    11401 aattcttaaa taactgctct gattatgaaa tagaagcaat tggaatcgcg ctacctggtc
    11461 gctatttaga tgattataaa ataacaacaa acaatccttt atggcaacat atcgacttag
    11521 aaatgattca atcccacttt gataagcctt tgtttttctc taacaatgtt aactgtatgg
    11581 ctataggtaa acgcctattt agtcgccaac aaaatgaccc taattttgct tattttcatt
    11641 ttgctagagg tatgcactgt tcttatatct acgacggaaa catttatggg aaaggaaatc
    11701 taatgattgg agaaataggg catacagttg tttcatctga gggagaagaa tgtagttgcg
    11761 ggcgcaaggg atgcttacaa acttttgctg gagaatcttg gttaatcaaa aaatcaaaaa
    11821 ttctatatca tcaatctccc tattctctac ttcctagttt agtcaaaaat gccgatgata
    11881 ttgatatcca agttatttta accgcttatc aattaggtga cactggaata atcacactta
    11941 tccatcaagc cttactctat cttagtcaaa ccattttaaa tatcagtatg atgattgact
    12001 ctcaaaaaat ctacctacat agtcctttac taacaaatca acacatcatc caaaaactat
    12061 atagtgaaat gaactacaaa cccaaactgc tctataatcg cctacctgaa gtcattattg
    12121 aaccttacaa tgattttaca gccgcacact cagctattgc actttgtttg tatcatacaa
    12181 ttctacattc ttaattttct attacattta ataaataggc taaaaagagg gagtatatca
    12241 taaaaagcga acaaacctgt tttctgaaaa tcagaattct gttttgttcg ctttctttat
    12301 atttggaatt atactcctat ctcccctttt tcataatgta tagtaaaatg aaataataac
    12361 aggacgaatc gatcgggaca gtcaaatcga tttctaacaa tgttttagaa gtagaggtgt
    12421 actattctag tttcaattca ctatattttc gtacaggtgc ttcaaccatt tgaacgattt
    12481 caaatccttc tttttggtaa agattctgag ctctttgatt tgcctcgaag acatttagag
    12541 aaatactatc tatatcttca ttttcaaagg ccaaactaac aaatttcctt aaagcctggc
    12601 tatctaagcc ttgcccctgt ttctgggggt tgataaaaaa tctcccgata tgaagattgc
    12661 tgtcttctag cctgattttc tggataagtc ccacaaactc ttgttcatca aagattgaaa
    12721 agactccttc caaggcttga agggtcagtg gaaaaggaat ccttggtccc atccattgtt
    12781 cttgaaagac tttgcctaag gagttggacc attggcatac gagctgagcg ttttctatac
    12841 tcaccttttc ttcaaaacga attgtcatct tttcctcacc atcttatcta tgtttctcca
    12901 ttatactatt tctcccattt tttacgaata gataagtatg attgattttt atttttttct
    12961 cgtcgggagc attctagctt cctttcttgg tttggtcatt gaccgttttc cagagcaatc
    13021 cattatcagt tcagccagtc actgcgattc ctgtcagact cccttgcgtc ccttagattt
    13081 gattccgatt ctctcacagg tcttcaatcg ctttcgctgt cgctactgca aagttcgcta
    13141 tcctgtctgg tatgccctct ttgaactagg cttaggactc ctctttctgc tttactcttg
    13201 ggaattgctt tccttggggc aagtcgtcct aatcaccgct ggtttgacct tgggtatata
    13261 cgactttcac catcaggaat atcccttact ggtctggatg actttccacc taatcctaat
    13321 agcttcctct ggctggaatc tggtcatggt ctccttcctc atacttggaa ttttggctca
    13381 ttttattgat atccgcatgg gtgcagggga tttcctcttt ttagcttctt gtgctctcgt
    13441 ctttagcgta acggagttac tgatcttgat tcagttcgct tctgcgacgg gtatcctggc
    13501 ctttctcctg caaaagaaaa aggaaagatt tcctttcgtg cctttcctct tacttgctac
    13561 ttgtttgatt atttttggta agctactgct tgtctgataa aatccaattt ctgccatatg
    13621 tccttcatga aattatttca cagttaaatt ataaattatt tcttttgtac aaagggatga
    13681 tgttatcaaa tcgatctgtt cttctatctt cttgatactg atcaaaaaat ttcatttcga
    13741 ctgaaaatat ttcgcttata aactgtaaac gaatactttg tttagacatt atagtcgcta
    13801 gactgactag atgattactc aaaacgacgt ccagaatact ctttactttg cttggttttt
    13861 taacaaaaat ttgatcatcc aaaggttcaa tcattttgta acctttttgc gcaatttgac
    13921 gataaaagta agaatgttgc tttggagtca ataatcctaa cttaaaagct cgatactcta
    13981 aagcctgtat cgaaacattc aaatccgact tcaataaaat ataactatca ggattgctga
    14041 cacgcttgcc aaccctctct tcaaatttga ctaaaaactc ttcttttggc aataaaaaac
    14101 atgatgcaaa ataatttgct tcttgctcca aacgatcgcc atcttcattc atatctttat
    14161 atttatgtaa aagaatatgt cctagctcat gagctaagtc aaaatttcga cgtacagatg
    14221 atttattcgt tcccaacaca atataaggtc ttcccaattt tgaccatgcg ctataagcat
    14281 cagcttggcc attaattaat cgttccacga tatagatgcc tgaacgttct aatttataaa
    14341 gcaaatcatg attatctttt gaaataccta atttttccct ggcataaaga gccaattcct
    14401 caatggattc tcccttatga taagattcac tcactacatt acttaggtca tgaattataa
    14461 tattaggtat aattacaaaa ctttcaaaat aatcaatcaa actatctacc ttatgtaaat
    14521 acatagtttg aatatctatt gttttccgtg ttgctaggtc tgcatttcta aaggcaatta
    14581 cagaagaatc aaatcgaatg ctctcttctt cctgttcaaa ataagttaaa tcaacatgaa
    14641 attggttggc caaatgcatt ttggttgata atttaggttt cgtttcgttg gactcaaact
    14701 gccaaatggc ttgttccgtt aaattaattc tctgagctaa ttctgctcta cttaaaccat
    14761 ttaacagccg taattctttc aatacccgac cattaaacat ttacatactc cttactactt
    14821 ttgaccttct tgtttttcta ttcttggaat aatttcaaaa tcttctgttt ccgataattc
    14881 tgaaaaatta ggaatatctt gatatttagc ttcttcgaaa tggtacgaac ttgattgaat
    14941 atactcagac aaatcttgaa tcaagtgtaa ctgaccagtt cgtgcatcag gcataacaac
    15001 atctacagat ataatattgt tttctgagtc cgcctcataa gttaaaatca taaatttttc
    15061 gatattcgaa tttttagtag cttgttcaat ttcttgaatc atttcatcag aaactaactc
    15121 catctgaatt ggaaaggaat gactatcttc atcatttttg taggaagaat gttgattaag
    15181 ataaagtgta ttcatctgag catattcaaa taagtagcca ctcttatttt tttgtaccaa
    15241 aggaaattgg ttcgtaagtc gcttcttacc ctttataatt aacaatactt tcccatattt
    15301 ttctgtattt gtttcaaatt ctaaatatcc ccaagtctgt cctgctaatt gtaatttata
    15361 ctcaaacaaa tctgctgatg caaatgcagt atcaatatga ttaggtcgcg tccatgcata
    15421 accattcgac actatcattg tctctctttt ttctagacgt tcatctacat aatctttttg
    15481 ccctttcatc aaagtatcta caattttttg tgcctcaagc gaatcaaaga gatcctgatt
    15541 caacataatt cttcctcctc caaatacttt ttaatgaatt atatcatttt cttaaagaaa
    15601 ttactacaat aattagcttt ttcttaaagt tctgtgtcag agtaatttag aaaattatat
    15661 cttctatagt aaaatcaatt aaaaactgaa caaatttatt gggaaattca aatcactttc
    15721 ttaaaatatt ttaggaaccg tagtgtaata ttccagattc aattcactat aaaactagcc
    15781 tttctcctgc aaaagaaaaa ggaaagactt cctttcgtgc ctttcctctt acttgctact
    15841 tgtttgatta tttttggtaa gctactgctt gtctgataaa atcctgaatc ggctctcctt
    15901 ggtggagagc ttttactatt tttgaaccga cgataacacc atctgacacc gcattgaagc
    15961 gttccagatc ggcttgacta gatacaccaa aacctgtcaa gactgggatg tcggccactt
    16021 gatgaagttg cgccaagtgc ttgtccaaat ctgcacggta attgcctgat ttccctgtca
    16081 ctccattgat ggcaacggca tagatgaatc cctccgcccc ttcaatcaac tctttctggc
    16141 gctcaattcc tgtggtcaag cttactaaag gaatcaaggc gatatctgta tctgccaaaa
    16201 atggttctac aaagttggca tgttcatgag gcaagtctgg gataatcaag cccttcacag
    16261 ctgtatcagc cagatctttg acaaagttct ccacaccgta ctgaaagagg ggattgaagt
    16321 aggtcatgat gaccagtgga atctctgttt caatggtttt caaggtttca actaaagcct
    16381 gggtagaggt cccgtgggct aaactgcgca agccagcttc ttcgataaca ggtccatctg
    16441 caacagggtc tgaaaaggga atacccactt caattgcaga gacacccaaa tcttctaaaa
    16501 agtgaattgt ttcagcaaga ccgtccaaac ctttctcgtg gtcaccagcc atgatatagg
    16561 gaacaaaaat tccttttcca gttgctttta tagcattcaa tttttctgtt agtgtcttag
    16621 gcatgagctt ctcccttctt tgctgcatct gcttccaagc ggtctttgac ttgaaccaca
    16681 tccttgtccc cacgacctga taggcagaca atcatagact tttctggtcc aagttctttg
    16741 gccaatttca ccgcaaaggc gatagcatgg ctagattcca aggctgggat aatcccttcc
    16801 acacgagaca agagttgaaa tccttccaaa gcttcttcgt ctgtcacagg gacatagctg
    16861 gcacgtttga tatcgtggta gtgagaatgc tctggaccga taccaggata gtccaaacct
    16921 gctgagatag agaaggcttc aagaatttga ccatgggcat cttggagcac atccatgagg
    16981 gaaccgtgaa ggacacctgg acgacccttg gtcaaggtag ctgcgtggtg ctccgtatcc
    17041 acaccaagtc cagccgcttc agctccatac atggctactg actcatcttc tacaaaggga
    17101 tggaagagac cgatggcatt cgacccacca ccaacacagg ctactagggc atctggcaga
    17161 ttttgtcctg tcaagtcacg gtactgttgt ttagcctctc gaccaatgac actttggaag
    17221 tcacgaacaa tttctggaaa tggatgaggc cccaaggcag aaccaaggat atagtgggta
    17281 tcatcgatat tagccaccca tgaacgaagg gctgcattga ccgcatcctt gagcacgcgc
    17341 gaaccatctg tcacggcctc gaccttagct cccaaaagct ccatacggaa gacattgagg
    17401 gcttggcgtt tgacatcttc ctcacccatg tagatggtac attccatgtt aaagagggcc
    17461 gcagcagttg cagttgccac accgtgctga ccagcacccg tttctgcgat aattttcttt
    17521 ttacccatgc gtttggcaag ccaaacttgt cctaaggcat tgttaatctt gtgggctcct
    17581 gtatggttaa ggtcttcccg tttgagataa atcttggctc cgccgatatg ctgggtcaag
    17641 ttttttgcgt agtaaagggg agtttcacgt cccacgtact ggcgcaaaag ttggtttaat
    17701 tcctcttgga aacttgggtc tgcctgactt tcacggtagg ccttctccaa ctccaaaact
    17761 gctgtcatca atgtttctgg gacaaaacgt ccgccgaatt ttccgtaaaa tccatcttta
    17821 tttggttcct gatatgccat gctttaccct ctctataaat cttctaatct tttcatgatc
    17881 tttttgtcca tctgtctcca ctccgctcga tacatctact gcatagggag taaaatgttg
    17941 aattgctttt actacattat cttcattaag gccacctgcg ataaagaagg gctgtgctag
    18001 tccagtcgta tccagttgac cccaatcaaa ggactggcca cttcctgcca caggggcatc
    18061 aaagagtaga taatctgcct gagaattggg gacatgccca tttccatcta cctgcacagc
    18121 ctgaatactg gcacaaggca aattctcaaa taaatcatct gccacctgac cgtgaacttg
    18181 aaccaagtcc aagtcaactt tgtcaatcgc ttccagcagt tctacccgac ttggtgaaac
    18241 aaatactcca acctttttca catctgcagg aataagcttt gccaactcag ctgcctcttc
    18301 taaagtcacc tgtcttttac taggtgcaaa gacaaaaccg atatagtcgg ctcctgctga
    18361 aacggctgtt tccaccgctt ctttggtcga tagtccacaa attttaacct ttgtcaatct
    18421 gcaactcctt gattctctgg gccacatttt ctgcctgcat aagagctgtc cctaccaaaa
    18481 ttccgttaaa gtatggggct agtcgttccg catcctgccc tgtgaaaatg gcagattcag
    18541 aaatgtaata gcgaccttcc tcaaagtaag gggctaaatc tacactggtc tgcaagtcga
    18601 cctcaaaggt agtcaagttg cggttgttga ccccgataat ctcagcacca agtctgtggg
    18661 ctacctctag ttcagctaga ttgtgagtct ccactaagac ttccagacca agctctgtcg
    18721 cgtagtcata cagttccttg aggcgttctt cggacaaggc tgccacaata agcaagataa
    18781 ctgtcgcacc tgcattgcga gcgcggatga tttgcttttc atctatgata aagtctttgt
    18841 tgagcgtcgg aatctctacc tgactggaaa tttcccgtag ataatccaaa tgccctttaa
    18901 agaaaacctc atctgtcaac accgaaatca tcactgctcc gttttcttca taagtctggg
    18961 cctgttgcac aatatccaca tcgagattga tatctcccaa actagggcta gctttcttga
    19021 cctcagcgat tacctgcaag cagtcctgat gattcttcaa aaattctgcc aagcgatagg
    19081 tctggcgcag aggctggatt tgctccagct tcatctgctc cacctcacgc gccttctgct
    19141 ctaagattcg tgctaaaaat tcctgactca tttttggtac tcctgtaaca gtctgagttt
    19201 ttcaagggcc ttgcctctag caatcacttg acgggccaag gcaactcctt ccttgatgct
    19261 atcaatctta ccattagcat agaaaccaag accagcattc aagactgtcg tttccaagaa
    19321 tggacttgct tcgtttttca gaacgctaag caaaatttct gcattttcct gagcattccc
    19381 accacgaata tcttccatag catagccttc cattcccaaa tcctctggag taaagcttga
    19441 caagctgatt tcgccatttt caagaagtgc aatcttggtt gttccgttca agccagcttc
    19501 atccaaccct tctggtccag caaccacgat ggcacgtttg cgacccatat ttttcaaaac
    19561 ctgagctgta ctttctagga gttctggacg actaattcca agaagctgtg tttctaaagc
    19621 cattggatga atcagtggac cagtcaagtt cataatcgtt ggaattccca attccaaacg
    19681 agctggcatg atgtatttca tagctgggtg catattttta gcgaagagaa agacgattcc
    19741 agttttatca aagaccttac ctagttcagc tggtttgagg tcaagattga ttcccaaggc
    19801 ttcgaggaca tctgcggaac cagatttaga agaaatcgag cgattaccat gcttggccat
    19861 gtgaataccg ccaccagcca agacaaaggc tgcagttgtg gaaatattaa aactgaaaga
    19921 cttgtcccca cctgtaccac agttgtccat ggcatcatga atctcagttg gaatatgctg
    19981 ggcatgtcct ctcatgactt gggcaatggc tgtgcgttct tcaggtgttt cccccttcat
    20041 cttaagagct aagaggagag aagcaatctg cgcttcagtt acacgcccag ttacgatacg
    20101 ctcaatgaca tccgtcattt ccacacctga taaattttca aattttgcta gtttttcaat
    20161 aatctctttc atcctagttt cctcacttta caaccttctc gataaaattc cgaatagaag
    20221 acaagccgtc tggcgttcca atgctctctg gatggtactg gaagccataa atcggtaggt
    20281 ttttatgttg aatccccatg atggcttggt catcagtcga acgagctgtc acttcaaagt
    20341 cttctggcat ttcctcaatc aaaatactgt gataacgcat gaccgcacgg ccatcctcaa
    20401 taccttgata caaaacagat ggcgcttcaa agttaatatt gctctgtttc ccatgcatga
    20461 cttttggagc caaacctagc ttaccaccaa agacttctgc aatggcttgg tgacccaaac
    20521 aaatccctag aatcggcttc ttgcctgcaa aatcacgaat catgtcttcc atctttccag
    20581 catcaactgg ccaaccagga ccaggagaaa agaccagacc atctgctttt tcagcttctt
    20641 catacagctt ggaatcatca tttctcagaa cctgaacttc tgcaaaattc ccaatgtatt
    20701 gggccaagtt ataggtaaaa gaatcatagt tgtcaatcaa taaaatcatg gtcttagttc
    20761 tccaattcta gtcatagatt ttgctttgtt aatggtttct tggtattcgt tttgggcgat
    20821 agagtcgtag acaatccctg ccccagcctg cacataggct ctttgatttt tgagaatcat
    20881 ggttcggatg gcgatggcca aatccatatc acccgtcgca gacaagtagc cgattgcccc
    20941 agcgtatact ccccgttttt ccgtttccag ttcatagata cgtctcatcg ctcgaatctt
    21001 tggcgctcca gaaacggttc cagcaggaag cgttgctttc aaggcatcca tggcagtgag
    21061 ttctgcaagc aaacgtccct tgaccacact ggtcaaatgc atgacgtagc ggaagagctc
    21121 cacctccata tacttagtaa cttggacact ggccgtttca gagatgcggc caatatcgtt
    21181 acgccccaag tctaccaaca ttcgatgttc tgctgtttcc ttctcatcag agaggaggtc
    21241 agtcgccaag gccttgtctt cttcatccgt agcccctctt ggtcgcgtcc ctgcaatcgg
    21301 attggttgtc acgatgccat ttttgacaga aaccaaactt tctggactag ctccgatgat
    21361 ttgataatcc ccaaaatcat agaaataaag gtaattagaa ggattagtta cgcggagatt
    21421 tctgtagaag tcaaatggat ttccagtaac ttctgctgag aaacgctggc tgagtacaca
    21481 ttggaacata tcgccgttac gaatcaagtc acgagctgtt tctaccattc cctcaaactt
    21541 atgtggagcg atatgcggtt tgaagtcaag tggtgataaa tccaagtctt caaattcatt
    21601 tggagcagga atgcgtaatt cctcaagcac ttggttcaag gatttttcca aggcctcttg
    21661 actgcgctca ctataaagtg catcctctat gacatgtatc ttctccttct tgtggtcaaa
    21721 gactatatag ctctcataga caaagaaatg catgtctggc gtcccaattg tatcctcagg
    21781 gatttgacca atttcttcat aaagcgaaat catatcgtaa cccacaaaac caatggctcc
    21841 accaccaaaa ggtagctctg aatggtgctg actcttatga atcacttcat aaaggaaatc
    21901 caagggatcc cgatcaatca cttgaccatt ttgataaaga actccatttt caaacttaat
    21961 ctcaaaaaca ggattatagg ctaggataga aaaacgagct gtttccttgt ctctcggaat
    22021 actctctaag ataaccttgt gttgcccctt taagcgcata taagccaaga ttggtgataa
    22081 gacatctcca tgaatgattc gttccattgt aatttccctt tcagttctaa ttctagttcg
    22141 tagcgactgt atgaaaaatc cccacgcaaa ataacttgcg tgaggacgaa attcgcggtg
    22201 ccacctcaat tataggattt ctcctatctc tcattcctgt ctcagatatc tcctgtaaca
    22261 ggctgtgcga taaagggcac tcccttgaga atgatgtttt cttctctcgt ttcagatgaa
    22321 cccaacttta cagctttctc tgcttgtttt cagcaaccac aagctctctg tgagagaaag
    22381 aactgtaatt tttccatcta ttatttttta gcttctagta gtctgcaatc gcagctaggt
    22441 ccttgcctcc acgaccagag acattgatga agagatgttc atctcggtac acctttatac
    22501 tcttcgaaaa tctcttcaaa ccgcgtcaac gtcgccttat cgtaggtatg gttactgact
    22561 tcgtcagttc tatctgcaac ctcaaaacag tgttttgagc tgacttcgtc agttctatct
    22621 gcaacctcaa aacagtgttt tgagcagcct gcggctagtt tcctagtttg ctctttgatt
    22681 ttcattgagt attactagct tttttcgtat tagtccagcc tttttgttta cttttagtag
    22741 gaggcaagag ctgtagatag aactcaagtt catcaaagcg acttaaggcc ctaataaaag
    22801 ataaagcaaa cgacggatag aaaaaagccc acacacagaa tatacttccg tgtgagggcg
    22861 ttggtaacgc ggtgccacct caattataaa gggactatcc ctttacatct ctgccttgtt
    22921 taacaacaag ctgcactgta aggtgtgcgc accgaatttt cattgtttca aattcatttt
    22981 caaaatcagc ccactttcac tacttccaac cacctattca caatcaccac aggctccctg
    23041 aagatcaaaa atagttactt ttctgatttg ttgaacttat tttaatactt tgttttttct
    23101 ttgtcaagac ttttttacga tttttttgaa aatatcattc gaatgtgacc atgtcttcct
    23161 tagatcgaac atgaacatgt cccacttgtt agaaattgga tccaactcaa tagaaactga
    23221 atggaggcta aacagaactt attttagaac actccatctt ttccactagg attttcaaga
    23281 attaaacaat actagaaact ctgtctccta acaaatttag gagaaacttc aacagatgtg
    23341 acactttccc ctttaataat cgctaaaaca ccttctatca tttctttagc caatttaaca
    23401 taattgggag caattgtaga caaagctgga gtataatact gagaaatagg aatattatca
    23461 aatccaatga tagaaatatc atctggaata agaattcctt tctcatagca cgcacgaatc
    23521 aagccctgaa ccttttcatc tcctgaaaca aaaataatgt ccggataatt ttggctagtc
    23581 aagtgctgca ttgcataaga ataaactgaa tcaattgtag ataagccata aatgactttt
    23641 aaatccataa agtaattttt atcattcaga aaagaacgca cacctctttc acgatcctta
    23701 ttaacatggg attctcctcc cataagcaac cacatatttt taaatttttc ttcagttaca
    23761 gctttcatca tatcataagt agcttgaaaa ttattattag atacatagac tactccagac
    23821 gtttgagatt caccgaaaac aagaaaaggc atatggttct tctttaaata ctgaattctg
    23881 atatcatcta cactttcata aaaaacaata acaccatcta ctaggctacc tgtgcttgat
    23941 ataattgaat tactaattgt atcctcctct ccaaagtact caactatagc attaacacca
    24001 aattctttac acgtccgtaa cactttatct aacagcgtat gaaaccaaat taaaggaaaa
    24061 gagtcgattt tttttacaga aatcaatata tttatagctt cttttttagt taaatttttt
    24121 gcatacgcat ttggaatata cgacaattcc tctataactt tttgaatcgc ttgataagtt
    24181 tcttctttaa catttactcc accattaata actcgtgaaa ctgtttttgg agaaaaacct
    24241 gataaacgtg caatatcata aatagttacc tttttcccat ttatattttt catttcagtc
    24301 ctccattacg aacattctaa tattactata caatatttaa
//
LOCUS       DAVKQL010000031        24106 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVKQL010000031 DAVKQL010000000
VERSION     DAVKQL010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24106
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.031"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            185..1666
                     /locus_tag="VW561_001195"
     CDS             185..1666
                     /locus_tag="VW561_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9943459.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENRDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVISNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSP
                     YDIIISNFIIPSIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            2028..3029
                     /locus_tag="VW561_001196"
     CDS             2028..3029
                     /locus_tag="VW561_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9943460.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     TVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILKKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
     gene            3179..4096
                     /locus_tag="VW561_001197"
     CDS             3179..4096
                     /locus_tag="VW561_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9943461.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAARLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            4107..4997
                     /locus_tag="VW561_001198"
     CDS             4107..4997
                     /locus_tag="VW561_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9943462.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            5026..6642
                     /locus_tag="VW561_001199"
     CDS             5026..6642
                     /locus_tag="VW561_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9943463.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVEDLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            6652..7971
                     /locus_tag="VW561_001200"
     CDS             6652..7971
                     /locus_tag="VW561_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU9943464.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEETGVRQVQNMAFSDDGIHFEKISQNPVATGSDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWREKRFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDTDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(8181..8253)
                     /locus_tag="VW561_001201"
     tRNA            complement(8181..8253)
                     /locus_tag="VW561_001201"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8218..8220),aa:Lys,seq:ctt)
     gene            complement(8518..9789)
                     /locus_tag="VW561_001202"
     CDS             complement(8518..9789)
                     /locus_tag="VW561_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEU9943465.1"
                     /translation="MPDNLALRMRPKTIDQVIGQDHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYAARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKASGHLPIPRHLRDGHYSGSKELGNAQNYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDHLRKI"
     gene            10059..10298
                     /locus_tag="VW561_001203"
     CDS             10059..10298
                     /locus_tag="VW561_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943466.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQEVHSNNGLKKYEK"
     gene            10463..10642
                     /locus_tag="VW561_001204"
     CDS             10463..10642
                     /locus_tag="VW561_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943467.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSGPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            10779..10958
                     /locus_tag="VW561_001205"
     CDS             10779..10958
                     /locus_tag="VW561_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEU9943468.1"
                     /translation="MPMTQKEMVKLLTAHGWIKTRGGKGSHIKIEKQGERPITILHGE
                     LNKYTERGIRKQAGL"
     gene            10995..11447
                     /locus_tag="VW561_001206"
     CDS             10995..11447
                     /locus_tag="VW561_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEU9943469.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            11738..12208
                     /locus_tag="VW561_001207"
     CDS             11738..12208
                     /locus_tag="VW561_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEU9943470.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNAEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDVLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            12227..13315
                     /locus_tag="VW561_001208"
     CDS             12227..13315
                     /locus_tag="VW561_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU9943471.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAILFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQFKVRKDVVQTHKAMKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            13296..13454
                     /locus_tag="VW561_001209"
     CDS             13296..13454
                     /locus_tag="VW561_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-dihydro-8-oxoguanine-triphosphatase"
                     /protein_id="HEU9943472.1"
                     /translation="MELEISDFPGCKIALFCGDKLLTILRDDKASIPWANMWELPGGG
                     KSVRENLI"
     gene            13564..14598
                     /locus_tag="VW561_001210"
     CDS             13564..14598
                     /locus_tag="VW561_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9943473.1"
                     /translation="MKKSDVLDLIKYHYEGRETEFRNQSIAIARNFNKHGDTQIAQYI
                     MGLMSQSDRFMPQIENPSEYLTPAKLDIGPLPLPLSIMNDLKGIINAVNHHIGINKFL
                     FVGSPGTGKTESVKQVARLIGKELLVVDFSHLVDSKLGQTVKNLATLFNEINNLPFKQ
                     NYIILFDEIDSIVLDRVNQNDLREMGRVTSAFLKELDRLSPEIVLIATTNLFENLDKA
                     VTRRFDAIIDFDRYTDEDKVEVATIILNELLKQFKNVARDLKLFKKIINSANVIPNPG
                     DLRNSIRTSLAFSDPSDPHDYQKRLLRSLHNGRNLSISKLSKLGFTVREIEILTGISK
                     SSVSRELSED"
     gene            14600..16843
                     /locus_tag="VW561_001211"
     CDS             14600..16843
                     /locus_tag="VW561_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008288790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEU9943474.1"
                     /translation="MNTLLTLRGKSFTPKSAKGRGPITIPADSIVTLNHLNELYTSLE
                     RVKEYWSNNTIIDGVLISVYYNRVLAKSNRIDGFFNVGGGNAIPNETIVGAKFSNDKS
                     RHIITHYISKEVLDKTIEHTKKVITIFKDYFASAPMTSMVFSDSSKFNQIDYSKYGLS
                     KSKFKQYLRDACFVKGFGIEQADMSTIRNSIVTIYDVKTNLLTLLKQLDIDVSEANIM
                     DQTTVLLDEKNIELLLLKAPYLVSMIVEDFSSLSPENFYNDGNTFYNNLPFPTNEPII
                     GVIDTLFDDRVYFNEWVEYHDLISPDISKGSQDYKHGTAVSSVIVDGASLNPKLDDGC
                     GRFRVRHFGISLQSGFNSFTIIKQIREIVSQNPDIKVWNLSLGSNDEIRENFISVEGS
                     LLDEIQFDNDVIFIIAGTNGIAPDGQRKKIGAPADSLNSIIVNSVDFSNQVVSYSREG
                     VVLSFFIKPDVSYYGGGNGDFINVCEPLGLARVAGTSFAAPFIARKMAYLIHIMGLSR
                     EEAKALLIDSAIPWNDKKTFADISLLGYGVVPIKIEDILSTPDDEIKFIVSDVSRAYE
                     TYNYDFPVPISKEHYPYVAKATMCYFPKCSRNQGVDYTNTEMQLSLGVLDKKGIKTIN
                     NDNQYSENAPGYVREKSARNIFRKWDNVKHLGEKFSSRKKAKPILNPSNPQWGMSVKT
                     IERLRNDDGYGIRFGVVVTLKELNGVNRIEDFIQQAELRGWLVNRLQVEAQVDLFNSL
                     NEEIEFE"
     gene            16894..17913
                     /locus_tag="VW561_001212"
     CDS             16894..17913
                     /locus_tag="VW561_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU9943475.1"
                     /translation="MNITYIVGNGLDLQYGLKTRYKDFYEFQNRTYISRKENEEEYSN
                     LIYESLFSDTVNDYENWSDFELSIGKLTKDNDLISSSIEIKEKFIDDFSEVVDDLREY
                     LRIQQEKNLEKGNAIDFISTLDNMRTSLPVINQPAIDKKYNENLYQHDIVNILTLNYT
                     NVIDKLYNESAKSFSNQLRTNNYKFHIAPPIHAHGTVDICTVLGVSDETQISNSFDEE
                     QKESLIKNLVLKNYRENMDVKNSDIIKNSDIIILYGVSLGETDGYIWNQIAEQSIRSS
                     VPVIIYHYVPHFDAGNPTRVKRLYRNVEDKFIQNSGIDLELEKKLRDNLIVVIGKTIF
                     NLMER"
     gene            17926..18876
                     /locus_tag="VW561_001213"
     CDS             17926..18876
                     /locus_tag="VW561_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEU9943476.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVATVESDLQARLAELTDFMDLGEVKMGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLTEDAYRLVKDEGYLIMSGIIKDKW
                     DMVRESAESAGFFLETHMVQGEWNTCVFKKTKDISGVIGG"
     gene            18878..19621
                     /locus_tag="VW561_001214"
     CDS             18878..19621
                     /locus_tag="VW561_001214"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEU9943477.1"
                     /translation="MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDSIIVAYEESAKEGEAAALLQAITGLDTGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     VGLGPRILRAETAPLYALSALSVLVELEK"
     gene            19632..21428
                     /gene="pepF"
                     /locus_tag="VW561_001215"
     CDS             19632..21428
                     /gene="pepF"
                     /locus_tag="VW561_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU9943478.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFITYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDATLTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKHSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIVAYSAELGE"
     gene            21674..22228
                     /locus_tag="VW561_001216"
     CDS             21674..22228
                     /locus_tag="VW561_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEU9943479.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLFDHLGNPSPEIRNVLLNQGL
                     HYLSKEKDTTGFSSQYGWVHSFAHGADLLTEVVCHPDFPINRVHEVFDILGQLFKRMS
                     IRFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVDFPIEEREDFYKFSNFRSCLVE
                     VYVQLDQRNSLQDELKEAIQSFQY"
     gene            22339..22998
                     /locus_tag="VW561_001217"
     CDS             22339..22998
                     /locus_tag="VW561_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEU9943480.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLGHLGIAFAFGLAIVVA
                     AYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLGNSGM
                     STASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLSLMA
                     MILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFLGTE
                     E"
     gene            complement(23082..23378)
                     /locus_tag="VW561_001218"
     CDS             complement(23082..23378)
                     /locus_tag="VW561_001218"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943481.1"
                     /translation="MFEVVDKTDEVRSKHCFEVADRTDEVRSKHCLEVADRTDEVRSK
                     HCLEVADRTDEVSLKHCFEVADRTDEVSLKHCFEVADRTDEVSLKHCLEVADGS"
     gene            complement(23440..23973)
                     /gene="queF"
                     /locus_tag="VW561_001219"
     CDS             complement(23440..23973)
                     /gene="queF"
                     /locus_tag="VW561_001219"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEU9943482.1"
                     /translation="MKRQLALVVFSGGQDSTTCLFWVMQHYETVEAVTFAYGQRHHLE
                     IQITREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEAWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
BASE COUNT         7614 a         4046 c         5085 g         7361 t
ORIGIN      
        1 ttgattctgt attcacgccc tcaatagacg gtaaaataag aaaattgttt atatattgtc
       61 tccgtagtgt tattatacga aataaaagat tttaggaaaa gtcgttatat catgctatac
      121 ctattctttg tggtataatt gcaagaggtt taatccgata atttataaag agagaaagat
      181 attcatgaga gatttattat ctaaaaaaag tcataggcaa ttagaattat tagaattatt
      241 atttgaacat aaacgttggt ttcatcgttc tgaactagca gagttattaa attgtacaga
      301 acgtgcagtc aaagatgatc tatcccatgt taaatctgct tttcctgact tgatttttca
      361 ttcttctact aatggtatac gcattattaa taccgatgat agtgatattg aaatggttta
      421 ccatcatttc tttaaacatt caactcattt ttcgatttta gaattcatct tctttaatga
      481 aggttgtcaa gctgagagta tttgtaaaga attttatatc agttcatctt cgctctatcg
      541 tattattagc caaatcaata aagtgattaa aaggcaattt caatttgaag tcagtctgac
      601 ccctgttcaa atcattggaa acgagagaga cattcgttac ttttttgcac aatatttttc
      661 agaaaaatat tatttcctag aatggccgtt tgaaaatttt tcatcagagc cactatctca
      721 attgttagaa ttggtttata aggaaacaag ctttccaatg aatttatcaa cgcatagaat
      781 gctaaagttg ctcctagtta cgaacctata tagaataaag tttggtcatt ttatggaagt
      841 agataaagat tcttttaacg accaaagttt ggatttttta atgcaggcag aaggaataga
      901 aggtgttgct cagagttttg aatcagaata caatatctct ttagatgaag aagttgtttg
      961 ccaattattt gtgtcttact ttcaaaaaat gtttttcata gatgaaagtc tctttatgaa
     1021 atgcgtaaaa aaggatagtt atgttgaaaa atcttaccat ctattgagtg attttattga
     1081 tcagatttca gtcaagtatc agattgaaat ggagaatagg gataatctga tttggcatct
     1141 gcataatacc gcacatctgt atcgtcaaga gttgtttact gagtttattt tatttgatca
     1201 aaaagggaat acaatcagga actttcaaaa tatttttcca aaatttgttt cagatataaa
     1261 aaaagagctt tctcactatt tagagactct tgaggtttgt tctagttcaa tgatggtaaa
     1321 tcatctatct tataccttca tcactcatac caaacatttg gtgattaatt tgctacaaaa
     1381 tcagcctaaa ctaaaggttt tggttataag taattttgat cagtatcatg caaaattcgt
     1441 tgcagagaca ctttcttatt actgtagcaa taattttgaa ctggaagttt ggactgaatt
     1501 agaattatca aaggaatctt tagaagattc accttatgat atcattattt ctaattttat
     1561 tattccttct attgaaaata agagactcat ctattcaaat aatataaaca cggtctcact
     1621 catatacttg ttaaatgcca tgatgtttat tcgattagat gagtaaccaa aaataacaaa
     1681 aaaacgctat ttcgcgtttg aactgtacct caaaagttag acagaaaaag tctaattttt
     1741 ggggtgtttt tgttaagaaa agaccttcaa tcatttaagc aaaatgtaca tctagattta
     1801 tagccctcta aaactcttgc tttttttgta gaagatacga aaaaagccat gaaaccaagg
     1861 cttttcctgt tttatgtaga tgtttacaca tgaaaattga agtattttca aaagctaatt
     1921 ggagtgattt tattcatgat gggataaatg atctggccaa aaatgattga gaaatagttg
     1981 cttaaaaagc atttgcatga tactatatat tgaaaggagg tgataatatg gtagaacaaa
     2041 gaaaatcaat taccatgaaa gatgttgctt tagaagcagg agttagtgtt ggaactgttt
     2101 cacgtgtaat taataaagaa aaaggcatta aagaagtaac tttgaaaaaa gtggaacaag
     2161 cgattaaaac tttgaattac attccagatt actacgctag aggaatgaaa aaaaatcgaa
     2221 cagaaacgat tgcaatcatt gtaccaagta tctggcatcc cttcttttca gaatttgcta
     2281 tgcatgtgga aaatgaagtc tataagagaa ataacaaatt actcttatgt tctatcaatg
     2341 gtacaaatag agagcaagac tatctggaga tgttgcgtca taataaagtt gatggagtgg
     2401 ttgccattac ctatagtcca attgaacatt acttgacgtc aggaattccc tttgttagta
     2461 ttgaccgcac atactcagat attgccattc cttgtgtttc atcagataat gatgcaggtg
     2521 gaagagaagc agctaaacaa ttaattagta aaggatgtca gcatttagcc tttgtgggcg
     2581 gacataatac aaccattaat gaaaccaaac gtcgtagaat ttcatttgaa aagtatgtcc
     2641 aagagcataa aatacctctt agtatttttg atttggatga gacagttact gactattatg
     2701 gaaagctaga gagtttcttg gaagaaaatt cttctataga tggaattttt acaatcaacg
     2761 attttacagc tttggatgtg attgaaatac taaaaaaaaa tggcaaacgt attcctcatg
     2821 atgtccaaat tattggatac gatggaatta aaatggctgg agatagagat tatctacttt
     2881 caactattga acaaccattg gaagaaatgg caaaagaagc ggttcgtatt ttgtttgata
     2941 taattgatgg aaagactgtt aatttgcaga caatcctacc agtaaaattt gttgaaggaa
     3001 aaacaacaaa aaatgaaaat aagtcttgac agaaagcgct atcaatgata gaatgaattc
     3061 agataaaaag atttattttt aaaacaaaaa tgaaacgttt caaaaaaaga aataaagaga
     3121 cagcgtcaag cgctagcttt tctagaaaaa aatgaaacgt ttcaaaaagg aggttgctat
     3181 gaatagcaaa gcgaagcaag tttctctttg ggaaagaatc aagaaacaaa aactcttgtt
     3241 attgatgact gtccccggtt tagttttaac ctttatcttt aaatacatcc ctatgtatgg
     3301 ggttttaatc gcatttaaag attacaatcc tttaaaagga attttaggga gtgattggat
     3361 tggtttttct gagtttacaa aattcatatc ctctcccaac tttggtatct tgttagccaa
     3421 cacattaaaa ttaagtatct atggtttatt gcttggcttt ttaccaccaa tcattcttgc
     3481 tattatgctc aatcaactct tgagtgaaaa agtcaaaaaa cgaattcagc tcattttata
     3541 cgcaccaaac tttatctcag tcgttgttat tgtcggtatg attttcctct tcttttcagt
     3601 gggaggacca atcaacaatt ttctttctat gtttggaatg aaggctgact tcttgacaaa
     3661 tccagacttc tttagaccct tatacatctt tagtggtatc tggcaaggaa tgggctgggc
     3721 ttcaacgctc tacacggcaa cattggtaaa tgtagatcca gccttagtag aagcagcccg
     3781 actggatgga gccaatatct tccaacgaat ctggcacatt gatattccag ctcttaagcc
     3841 tattatggtt atccaatttg ttttagctgc aggtggaatt atgaatgtcg gatatgaaaa
     3901 agcattcttg atgcagacat cgttaaattt gccaacttct gaaattatct cgacatatgt
     3961 ctataaagtt ggtcttgtat caggagacta ttcttactca acagcggttg gtttgtttaa
     4021 tgcagtgatt aacgtagtat tgcttgttgc agttaaccaa atcgttaaac gcatgaataa
     4081 tggtgaagga atttaaggag ggaagtatga aaaattcgat tatggataca aaatttgata
     4141 gacgtatctt actcttaaat aaaatcatta ttgtctttat cgttttgatg actttgcttc
     4201 ctttacttta tatcgtcgta gcatccttta tggatcctaa ggttctggtt agtagaggga
     4261 ttagctttaa tccagccgat tggactgtag aaggttatca gcgtgtattc agtgaccaat
     4321 ctattctaag aggttttatc aattctctac tatactcttt tggatttgca gctttaacag
     4381 tattgctatc tgtgtttaca gcttatcctc tttctaagaa agacttggtt ggacgtcgtt
     4441 ggattaacta cttcttgatt gtgactatgt tctttggtgg tggtttagtc ccaacttact
     4501 tgctcgtaaa agaattggga atgctcaata ctccatgggc tatcattgtt ccaggtgctg
     4561 tcaacgtttg gaatattatt cttgctaggg cctatttcca aggattgcct gaagaattag
     4621 ttgaagctgc tgtcattgat ggtgcaaatg atttacagat tttcttcaaa atcatgcttc
     4681 ctcttgcaaa accaattatg tttgttctct tcctttatgc ttttgtagga cagtggaact
     4741 catactttga tgcaatgatt tatatcaagg atccaaactt ggaaccattg caacttgtac
     4801 ttcgtaaaat tctcattcag agccaaccag gtcaagacat gattggagca caagcggcta
     4861 tgaatgaaat gaaacgttta gctgaattga ttaaatacgc aactattgtc atttccagct
     4921 tgccattgat tgttatgtat ccattcttcc aaaaatactt tgataaagga attatggctg
     4981 gttcacttaa aggataaaaa aagaaaaaat aaaaggagtt ttctcatgaa attcaaaaca
     5041 ttctcaaaat cagcagtttt gttgacagct agtttagcag tacttgcagc ctgtggctca
     5101 aaaaatacag cttcaagtcc agattataag ttggaaggtg taacattccc gcttcaagaa
     5161 aagaaaacat tgaagtttat gacagccagt tcaccgttat ctcctaaaga cccaaatgaa
     5221 aagttaattt tgcaacgttt ggagaaggaa actggcgttc atattgactg gaccaactac
     5281 caatccgact ttgcagaaaa acgtaacttg gatatttcta gtggtgattt accagatgct
     5341 atccacaacg acggagcttc agatgtggac ttgatgaact gggctaaaaa aggtgttatt
     5401 attccagttg aagatttgat tgataaatac atgccaaatc ttaagaaaat tttggatgag
     5461 aaaccagagt acaaggcctt gatgacagca cctgatgggc acatttactc attcccatgg
     5521 attgaagagc ttggagatgg taaagagtct attcacagcg tcaacgatat ggcttggatt
     5581 aacaaagatt ggcttaagaa acttggtctt gaaatgccaa aaactactga tgatttgatt
     5641 aaagtcctag aagctttcaa aaacggggat ccaaatggaa atggagaggc tgatgaaatt
     5701 ccattttcat ttattagtgg taacggaaac gaagatttta aattcctatt tgctgcattt
     5761 ggtatagggg ataacgatga tcatttagta gtaggaaatg atggcaaagt tgacttcaca
     5821 gcagataacg ataactataa agaaggtgtc aaatttatcc gtcaattgca agaaaaaggc
     5881 ctgattgata aagaagcttt cgaacatgat tggaatagtt acattgctaa aggtcatgat
     5941 cagaaatttg gtgtttactt tacatgggat aagaataatg ttactggaag taacgaaagt
     6001 tatgatgttt taccagtact tgctggacca agtggtcaaa aacacgtagc tcgtacaaac
     6061 ggtatgggat ttgcacgtga caagatggtt attaccagtg taaacaaaaa cctagaattg
     6121 acagctaaat ggattgatgc acaatacgct ccactccaat ctgtgcaaaa taactgggga
     6181 acttatggag atgacaaaca acaaaacatc tttgaattgg atcaagcgtc aaatagtcta
     6241 aaacacttac cactaaacgg aactgcacca gcagaacttc gtcaaaagac tgaagtagga
     6301 ggaccactag ctatcctaga ttcatactat ggtaaagtaa caaccatgcc tgatgatgcc
     6361 aaatggcgtt tggatcttat caaagaatat tatgttcctt acatgagcaa tgacaataac
     6421 tatccaagag tctttatgac acaggaagat ttggacaaaa ttgcccatat cgaagcagat
     6481 atgaatgact atatctaccg taaacgtgct gaatggattg taaatggcaa tattgatact
     6541 gagtgggatg attacaagaa agaacttgaa aaatacggac tttctgatta cctcgctatt
     6601 aaacaaaaat actacgacca ataccaagca aacaaaaact agaggttgat tatgggagat
     6661 aagaaataca cagtagaaaa agccaatcgt tttatagcag aaaataaaca tctcgttaat
     6721 actcaatata agcctgaaga acatttttca gctgagattg gttggatcaa tgatccaaat
     6781 ggatttgtct attttcgtgg agaataccat ctcttttatc aattctatcc atatgatagt
     6841 gtttgggggc ctatgcactg gggacatgct aaaagtaagg acttggtgac ttgggagcac
     6901 ttgccagtgg cacttgctcc tgaccaagat tatgaccgaa atggttgttt ctcaggctct
     6961 gccattgtca aggatgatcg cctctggctc atgtacactg gacatatcga agaagaaacc
     7021 ggtgtccgcc aagtgcaaaa tatggcattt tcagatgacg ggattcactt tgaaaagatt
     7081 tcccaaaatc cagttgcaac tggatcagac ttgccagatg agttgattgc tgctgatttc
     7141 cgtgatccaa aactctttga aaaagatgga cgctattact ccgtagtagc tgccaaacac
     7201 aaggataatg tgggctgtat cgttctacta gggtccgata acctagtaga atggcagttc
     7261 gaatccatct ttttaaaagg aggagaacac caaggcttta tgtgggaatg cccagattac
     7321 ttcgagttag atgggaaaga ttgccttatt atgtcaccca tgcgttatca gcgtgaggga
     7381 gactcatatc ataacatcaa ctcatcgctt ttgttcacgg gtaaggtaga ttggagagaa
     7441 aaacgtttta tcccagaatc agttcaagaa attgatcatg gccaagactt ctatgcgcct
     7501 caaacattgt tggacgatca aaatcgtcgt atcctgattg cttggatgca gacatggggg
     7561 cgtacccttc caacccatga ccaagaacac aaatgggcat gtgccatgac tttacctaga
     7621 attctaagat tggaagatgg caaactaaga caattccctg ttaaaaaagg ccaatatcaa
     7681 atccaaatag ataaagattg tcattaccac ttaggaaatg atacagatta tcttgaattt
     7741 ggttatgaca gtaatgcgca gcaagtttac attgatcgta gccatcttat tcaaaaaatt
     7801 ctaggtgaag aagaacagga cactagtcga cggtatgtag atattgaagc taaagaattg
     7861 gaagttgttc tagataaaaa ttccatcgag atttttgtca atcaaggtga agcaagcttg
     7921 actgcaactt attacttaac ggtgccagct gagctatcac gaattgatta aaaattaagt
     7981 tatttctcct aaagaaaaag ttctctttct aaaatagtgg aaagaggact ttttgtgttt
     8041 tgggtatata agcttagttt atggtatttg taaaattggt gttggattat gatttaaact
     8101 agttttctaa agaatttgaa aaaaatttta tttaagcaaa aaaagcccta tattataagg
     8161 cttttcctgt tgtatttaga tgccccctac agggatcgaa cctgtgaccc acggattaag
     8221 agtccgctgc tctgccagct gagctaagga ggcaaagaaa aaagctgtat tggtgccgaa
     8281 acttcacggt ttgtattgaa cccgcgcaat taagcaggtg ggcaactcgc tctaactgaa
     8341 gctgtttccg tgtgagacgg cctacatgct gttagaagac ttttgtttcc ctaataatac
     8401 aaaaaatagt cggtcaacac ttaagtgtga agtcgtacac cacagcgttt ctatatttat
     8461 atgataccac tttttcaaaa aaaatcaaga ggaaagtgca gttttttgaa aaggatttca
     8521 gatttttcgc aaatggtcga tagcttcctt tctttgagcc aaagcccgtt catatttacc
     8581 agtatcttct gcttggaaat agtgatgatt acgaattttt tctggcagat agtcttgctt
     8641 gacccaattt ccaggatagt tgtgtggata gagatagttt tgggcattcc ccagttcctt
     8701 gcttccactg tagtgcccat cacgcaggtg tcgcggaata ggcaagtgcc ctgatgcttt
     8761 gaggtcagca agtgccttat ccatagctac ataggctgag ttggattttg gagaaagggc
     8821 caaatcaatc acgacattgg cgatgagaat gcgggcttct gggaaaccaa tcctttgtgc
     8881 agcatccaga gcagtcacgg tatgaatctg ggcctcagga ttggctaaac cgatatcttc
     8941 ataggcgata acagtcaagc gacgagcgag actaggcaga tccccagcct caatcaagcg
     9001 ggcagcatag tggagactgg catccacatc tgagccacgg atagactttt gcagggcaga
     9061 tagaacatca tagtgaccgt ctccatcctt gtccatagtg atgtagcttc tctgaagact
     9121 attttccatg atgtctaagg tgatgtggcg aatgccctcg tcattctcag gggtagagag
     9181 aacagccaag tccagtgagt taaaggcaga gcgaaggtct ccgtttgtag aggttgcgat
     9241 gaaatccagc gcatcctcat ctagttctat tggaaaatca aaaccacgtt cagggttact
     9301 tagagctatc tgcagggcct ctttgacgtc ttggttagac agaggttcca actcaaaaat
     9361 ttgaacacgg ctacgaatgg caggagtgac ggaaaagaaa ggattttcag tcgttgctcc
     9421 aatcatgatg accagtccac tttccaagag aggcaagagg aagtcttgct tggtcttatc
     9481 tagtcgatga atttcgtcta gcaataggac gagaccacca gaaaatttag cttcttccga
     9541 gatttcttgc agtcgctttt tactatcaac tgtcgcattg aaagttcgaa aggcatactt
     9601 ggtcgttcca gcgatggcag aggcaatact ggttttgccg attcccggag ggccatatag
     9661 aatcatggag gacaggcggt tggcttccac catgcggcgg atgatttttc caggtccgac
     9721 cagatgatcc tgaccgatga cctggtcgat ggttttaggg cgcatgcgaa gcgcgagatt
     9781 gtctggcata gcagtccttt ctaacgtgga tttgagtaga atgactcatc tactatatct
     9841 tactatgtcc attctagcct attagttgtg atatttcaat ggttttgtaa taatcaggga
     9901 gtgagataga atctttttta ctatttaatt taatgaggag aggtatcagc atgagagatt
     9961 tgattttcct taaagctcaa aataggatag ttgagcctaa agaagtggaa gtgctgactt
    10021 gagaaataat attgctaaat gtcattgtat gaactataat gaaaagagag gtgataagca
    10081 atggaaatga tggaccaagt caggaaattt tgatttttac aaaacaaatt cgtcattgga
    10141 ttcttagtga tcaagttatt tcagggaaaa gaaaactttt cttccgggaa gatactccaa
    10201 aagagatttt agatttgtac gaaaacatta aatctaaact tgattttgct taccaagaag
    10261 ttcattctaa taatgggttg aaaaaatatg aaaaatagaa taattgatgt ttttgaagta
    10321 gtaaatcgcc tccttgtaat aactgttgaa aatcctgatt ttgaagactt gagagttaat
    10381 catactcaat gaaaatcaaa gagcaaacta ggaaactagc cgcaggctgt acttgagtac
    10441 ggtaaggcga cgttgacgtg gtttgaattt gattttcgaa gagtatcagt ttgtaaaaat
    10501 tggagataaa aaatatagag tacgcagtgg tcctatgatt cattcaactc cgcctcaatc
    10561 tgtcttagat cgagatactt ttacaattga ctatacagat gatgaattgc tcgataaaga
    10621 agcagttttt actactcatt agaaaaatag gtctgaataa attggcttcc cttagaacca
    10681 catctagggg cttttatgta taatagaata ataagtatca ctcctttaca atacgtgtaa
    10741 cacgtgttat aatggtgttg tcaggaggta aaagcgctat acctatgaca caaaaagaga
    10801 tggttaaact ccttacggcc catggttgga taaagactag aggcggtaaa ggctcccata
    10861 ttaaaatcga gaagcaaggg gaaagaccta tcacaatcct tcatggtgag ctgaataagt
    10921 acactgaaag agggataaga aagcaagctg ggttgtaaaa ttcagtccct gctttttgat
    10981 atggaggtag tgaaatgtta gttacgtatc cagctttatt ttattatgac gacacagacg
    11041 gaacagaagc gacttatttt gtccatttcc cagattttga atactcagct acacaaggag
    11101 aggggatttc tgaggctttg gctatggggt cggagtggct agggataact gttgcagatt
    11161 tgatagagag tgatggagag ttacctcagc catcagatat caatagcttg tctttaattg
    11221 ataatgatcc ttttaaagat gatgaagatt tcgtgtcaac ctatgacctt gataaatctt
    11281 ttatttctat ggtatctgtt gatgtatcag aatacctagg aagtcaggaa cccattaaaa
    11341 agactttgac tataccaaaa tgggcagata agttgggacg agaaatggga cttaactttt
    11401 ctcagacttt gacagacgct attgcagata agaaagttca agcctaagat taagcgtggt
    11461 atcgtggtaa tagtcaataa aaaagcacgt ccacttgtgc tagttactcg cctattgatt
    11521 ttatagattt attagccctt tcgagggctt tttatattga tttttatgag aaatgaagaa
    11581 atcagacttt taagaaaatc actgatatca agggtttaaa tgaggtaata tggtataatt
    11641 aggacataaa ataattttgt ggtaagatgg tagtatctat tttagcatat ttccgagcaa
    11701 tcggggcgat taaagagtcg catagaaaga ggacaaaatg gcaacatacg gatttttaga
    11761 tgttttagag gaagagttgg acaagaactt tccctttgac tttgagatta gctgggataa
    11821 acgcaaccat gcggtagaag tgagttttct attggaagcg caaaatgctg caggtgtgga
    11881 gatggtggat gaagacggag aggtttcatc agatgacatt ctttttgagg aagcggtcct
    11941 tttctacaat cctgctaagt caaccgtcaa tgcagaagac tatttaacgg ttatccctta
    12001 tctacctaaa aaaggatttt ctcgcgagtt tttagcttat tttgcccttt tccttaaaga
    12061 tactgctgag gttgggctag atgtcctcat ggactttttg gaagatccag aagcagagga
    12121 atttgttatg gaatggaatc aagaagtctt tgaagaagga aaagttggct tggaagaggg
    12181 agaattttat ccttatccaa gatactagga gttggttgga ggtgttatga agaaaattgg
    12241 gacttatttg gtttatgtgc tagctgttgt ctttattatg ctagcttttg cttgtggaac
    12301 aatcgcattt gcagagttgg gatattccgc agttttagcc tttacttttg gttatgcctt
    12361 cgctcttcta agcatgtatt taatctttat tcttcatgag ctgggtcatg ctttttgtgg
    12421 ctacttgaca ggctatcggc tggtggcttt tggattagga cattttattt tgaccaaaaa
    12481 gtcaggcagg tttcatctta gtagaacagc cattctgaaa aatgttggtg ctcaatacat
    12541 tggtttaaaa gaggatgaaa gcgatcaaag aatcatcctg atgctttcag gaggcttgat
    12601 ggttcatctc ggcttgatat tattggcgat attgtttgga tttttgacaa gaagctggta
    12661 ttttgcaggg acttggattt ttcttaattt atctttcttc ctaaataaca ttttaccagt
    12721 cgacatcacc gatggagcaa aaatttggga attactacaa caccctgaaa ataccaaata
    12781 cgcctacctg gtattgagac attctgccca gaccttgctg gctcctcaag aatatgattt
    12841 gaaagacttt gttcaagcag ttcctgagga tgcacgaggg agttttgcgg atggtgtatt
    12901 aggtatgcaa ggtgaagttt ccattttgga agggaaggaa gaagtagcta aacagcagtt
    12961 tcaagcatta cttgagagga caactactcc gatgatgcag acagttgccc aactatccct
    13021 gcttcatatc gccttgttgg aaggagattt tgagcaagcg gagcaatatg ccagtattcg
    13081 acaggtcaaa tcgtttctgt ctctcaaact gtccaatctt caagtgatac aagcctggta
    13141 tcaatttaag gtaaggaaag atgtggttca aactcacaag gctatgaaga ttgctagaca
    13201 gaaaatggat agtagtcgaa tgttgaggga cgagaaacgc tattatgaaa acttgttagc
    13261 agagctggaa aaggaaatgt tagaaggaac tgaagatgga actagaaatt tctgatttcc
    13321 caggctgtaa gattgctttg ttttgtgggg ataagctttt gactatctta cgcgatgata
    13381 aggcaagcat tccctgggcc aatatgtggg aactgccagg tgggggaaag agtgtcaggg
    13441 aaaatctgat atagttttgc attaaaagaa tatcctttca aggttattct tttttaattt
    13501 tgtcccaaaa taattgactt tgtcccgaaa atatagtatt atttgtataa ggaggaatga
    13561 atcatgaaaa aatcagatgt tcttgattta ataaagtatc attatgaggg cagagagaca
    13621 gaatttagaa atcaatccat agcaattgct cggaacttca ataaacatgg tgatacacaa
    13681 atagcacaat atattatggg gttaatgtct caaagtgata gattcatgcc acaaatagaa
    13741 aatcctagtg agtatttgac tcctgctaaa ctagatattg gtcctctccc attgcctctt
    13801 tcaattatga atgatttaaa aggaataata aacgctgtca atcatcatat tggaataaat
    13861 aaatttttat ttgtgggatc tccaggtact ggaaaaactg aaagtgtaaa gcaggtggct
    13921 agacttattg gcaaggaatt acttgtagta gatttcagtc acttagtaga tagtaagcta
    13981 gggcaaacag tcaaaaactt agcaactctt tttaatgaaa tcaataatct tcctttcaag
    14041 caaaattata tcatcctatt tgatgaaatt gattccatag tgttggatag ggttaatcag
    14101 aatgatttaa gagaaatggg acgagtgact tctgcctttt taaaggagtt ggataggctg
    14161 tcaccagaaa ttgtattaat tgcaacaaca aatctttttg aaaatcttga taaagcagtt
    14221 acgagaagat tcgatgccat aattgacttt gaccgttata ctgatgaaga taaagtagaa
    14281 gtagctacta ttattttaaa tgagttatta aagcaattta agaatgtagc tagagattta
    14341 aaattattta aaaaaattat caatagtgcc aatgtaatac cgaatcctgg tgatttgaga
    14401 aactcaataa gaacgtcatt ggcatttagt gacccatcag atccgcatga ttaccaaaaa
    14461 cgcctgctaa gaagtttgca taatggtaga aatttatcta tttctaaatt atcgaagcta
    14521 ggttttactg ttagagaaat tgagattttg acaggtattt ctaagagtag tgtatcacga
    14581 gagttaagcg aggattaaaa tgaatacatt attgacatta cgaggtaaaa gttttacacc
    14641 aaagtcagca aaggggaggg gaccgataac gattcccgca gattctattg ttacgctaaa
    14701 ccatttaaat gaactttata catcccttga acgagtaaaa gaatattggt ctaataacac
    14761 aatcattgat ggagtactta ttagtgtgta ttataatcgt gtcctagcta agagcaatcg
    14821 tatcgatggt ttttttaatg tgggtggcgg aaatgcaatt ccaaatgaaa cgattgtggg
    14881 tgcaaaattt agtaatgata agagtaggca tattattaca cactacattt caaaggaagt
    14941 gttagataag acaattgagc atactaaaaa agttataaca atattcaaag attattttgc
    15001 tagtgctccc atgacgagta tggtgttctc cgactcttca aaatttaatc aaattgatta
    15061 ttctaagtat ggtctgtcga aatcaaaatt caaacaatac ttaagagacg cttgctttgt
    15121 caaaggattt ggtattgaac aggcagatat gtctactata cgtaattcta ttgtaacaat
    15181 ctacgatgta aaaactaatc tcttaacatt acttaaacaa cttgatattg atgtttcaga
    15241 agctaacatc atggaccaga caactgtttt acttgacgaa aaaaatattg agttactact
    15301 gctaaaagca ccttatctag tatcaatgat agtagaagat ttttctagcc tgtcaccaga
    15361 gaatttttat aatgatggga acacttttta taataatctc ccttttccaa ctaatgaacc
    15421 tattattggt gtcatcgata cgttgtttga tgatagagtt tatttcaatg agtgggtaga
    15481 gtatcatgat ttaatttcac cagatattag caaaggaagt caagactata agcacggtac
    15541 agcagtttcc tcggtaatag tagatggagc tagtttgaat ccaaaactag atgacggatg
    15601 tggaagattt agagtcagac attttgggat atctttacag agtggtttca attccttcac
    15661 aattatcaaa cagattagag aaattgtgtc tcaaaatcct gatattaagg tttggaacct
    15721 ttcgcttgga tcaaatgatg aaattaggga aaactttatt tctgtcgagg gttcgttgtt
    15781 agacgagatt caatttgata acgatgtaat ttttattatt gcaggcacta atgggattgc
    15841 ccctgatgga caacgaaaaa agattggagc accagcagat tcactcaatt caattattgt
    15901 aaattctgtt gattttagca atcaagtcgt ttcttattca cgcgaaggag ttgtcctgtc
    15961 tttctttatc aaacctgatg tttcatacta tggcgggggg aacggagact tcataaatgt
    16021 ttgtgaacca ctagggctgg ctcgagtagc tgggaccagt tttgcggctc catttattgc
    16081 tagaaaaatg gcttacctca ttcatattat gggattaagt cgtgaagaag caaaggcttt
    16141 attaattgat tcagcgatac catggaatga taaaaaaaca ttcgcagaca tttctttact
    16201 tggatatggg gttgtgccaa tcaaaataga agatattttg tctacacctg atgatgaaat
    16261 taaatttata gtctcagatg tttctagagc atatgaaact tataattatg atttcccagt
    16321 tccaatttct aaagaacact atccatatgt agcaaaggcc actatgtgct attttccaaa
    16381 gtgttctaga aatcaaggag ttgactatac taatacggaa atgcaactta gtttgggagt
    16441 tttagataag aaaggcatta aaacaattaa taacgacaat caatattctg aaaatgcacc
    16501 aggatatgtg agagaaaagt cggcacgaaa tatttttagg aaatgggata atgtcaaaca
    16561 tttaggagaa aaattttctt ctcgtaaaaa agctaagccg attctgaatc cttctaatcc
    16621 tcaatgggga atgagtgtaa aaacaattga acggttaaga aatgatgatg gttatggtat
    16681 tcgttttgga gtagttgtta ctctaaagga gttgaatggt gtcaatagaa tcgaagattt
    16741 tattcaacaa gccgaattac gcggttggct agttaataga ttgcaagtag aagctcaagt
    16801 agacctcttt aatagtttaa atgaagaaat tgaatttgaa tagtggatat tttatcctta
    16861 ttctagatct taggaacaca agagggcaaa gatatgaata ttacttatat agttggaaat
    16921 ggtttggatt tgcaatatgg tttgaaaaca agatataaag atttctatga atttcaaaat
    16981 aggacttaca taagtaggaa agagaacgag gaagaatatt caaatctcat ttatgagtct
    17041 ctattttctg atacagtaaa tgattatgaa aattggtcag attttgaatt gtcaattggt
    17101 aaattaacaa aagataacga cctcatctcc tcaagtatag aaataaagga gaaatttata
    17161 gatgatttta gtgaagtagt tgatgattta agagaatatc tacgcataca acaagaaaaa
    17221 aatcttgaga aaggtaatgc aattgacttt ataagcactc tagataatat gaggacctct
    17281 ctccctgtaa ttaatcaacc tgctatcgat aaaaaatata atgaaaatct atatcaacac
    17341 gatatcgtga atatattaac tcttaattac acaaatgtaa tcgataaatt atataatgag
    17401 tcagcaaaat catttagcaa tcaattacga actaataatt ataaatttca cattgcgcct
    17461 ccaattcatg cacatggaac ggtagatatt tgcacagttt taggagttag tgacgagaca
    17521 cagatttcaa atagttttga tgaagaacaa aaagaatcct taataaaaaa tttagttttg
    17581 aagaactata gagaaaatat ggatgttaaa aatagcgata ttataaaaaa ttctgacatc
    17641 ataattcttt atggggtttc tttgggagag actgatgggt atatctggaa tcagattgct
    17701 gaacaatcaa tcagaagttc tgttcctgtc ataatttacc attatgtacc tcattttgat
    17761 gcaggaaacc ccactagagt aaaacgctta tatagaaatg tagaagacaa attcatacaa
    17821 aatagtggaa ttgatttaga gttagagaag aaattaagag ataaccttat tgttgttatt
    17881 ggaaagacta tttttaatct gatggaaagg taagcaaaat acataatgga aacatggcaa
    17941 gagttaaaag ttacagtgaa gcgtgagggg gaggaattgg tttccaatct cttgatcgag
    18001 ctgggagcgc aaggcgttgc gattgaagac agcatggact atgtgggaaa tgttgaccgc
    18061 ttcggcgaga ttttcccaga ggttgagcag caagaagaaa tcgtagtgac agcctattac
    18121 cctgatacgg ttgatgtagc aacggttgag tcagatttgc aggctcgtct agcagaattg
    18181 acagatttta tggatttggg tgaagtcaag atggggacga ctgccttggc tgaggaagac
    18241 tgggcagaca actggaagaa atactatgag ccagctcgta tcactcatga tttgaccatc
    18301 gttccctctt ggacagacta tgaggcgact gcgggagaaa aaattatcaa gctagatcct
    18361 gggatggctt ttggtactgg gacccatcca actaccaaga tgagcctctt tgccttggaa
    18421 caggttcttc gtggtggcga aacggtgcta gatgtgggaa ctggttcagg ggttctctct
    18481 attgccagct cgcttctggg ggctaaagaa attttcgcct atgacctgga tgatgtggca
    18541 gttcgtgtgg ctcaggaaaa tattgagctc aaccctggaa tggaaaatat ccatgtagcg
    18601 gcaggcgatt tgcttaaagg cgtggagatt gaagcagatg tcatcgtggc aaacatcttg
    18661 gcggatatcc tcattcatct gacagaggat gcttatcgct tggttaagga cgaaggctac
    18721 ctgatcatga gtggcattat caaggataag tgggacatgg tgcgcgagtc ggctgaatca
    18781 gctggatttt tcctcgaaac tcacatggtt caaggggaat ggaatacctg tgtctttaag
    18841 aaaacaaagg atatctctgg tgtgattgga ggctagcatg cagcagtatt ttgtaaaagg
    18901 cagtgctatc tctcctgtca ctatcgagga caaggaaacc agtaagcata tgtttcaggt
    18961 tatgcgcttg aaagaagagg atgaggttac cttagtcttt gatgatggca tcaagcgctt
    19021 ggcgcgcgtg ctggatatgg aaaatcgtca gtttgagttg gtccaagaat taaatgacaa
    19081 tgtggaacta ccagtccaag tgaccatcgc atctggattt cccaagggag acaagttgga
    19141 gttcatcgct caaaaagtaa ccgaactggg tgctagccaa atctgggcct ttcctgcaga
    19201 ctggtcagtt gccaagtggg atggcaagaa attgggtaaa aaagttgaaa aactagaaaa
    19261 aattgccctt ggagcagccg agcaaagcaa gcgtaatatt gtaccaagta ttaaactttt
    19321 cgagaaaaaa gcagattttc tagctcagct ggaccagttt gactctatca tagtagccta
    19381 tgaagaatca gctaaagaag gagaagccgc tgcgcttctg caagcaatca ctggtcttga
    19441 cacaggagcc aaactgctct ttatctttgg tccagaaggc ggtctctcac ctgcagaaat
    19501 cgaaagtttt gaagctaaag gagcagtttt ggtaggccta ggtcctcgta ttttgcgagc
    19561 agaaacagca ccgctttacg ccttatcagc ccttagtgtt ttagtagaat tagagaaata
    19621 agaggaagaa aatggaacaa aaacaccgtt cagaatttcc agagaaggaa ctctgggact
    19681 taacagccct ataccaagac cgtgaggatt tcttgcgtgc aatcgagaaa gctcgcgaag
    19741 acatcaacca gtttagccgt gattacaagg gaaatcttca cacttttgag gatttcgaga
    19801 aggcctttgc ggaattggaa cagatctaca ttcagatgag ccatattggc aactatgctt
    19861 ttatgcctca gacgacggac tatagcaatg acgaatttgc caatattgcc caagctggga
    19921 tggaatttga aacagatgcc agcgtagcct tgaccttctt tgacgatgcc ttggtggtag
    19981 cagacgagga agtcttggac cgtttgggtg aattgcctca cttgacggca gctattcgtc
    20041 aggccaaaat caaaaaagcc cactacttag gggctgatgt ggagaaggcc ttgaccaatc
    20101 tcggtgaagt tttctatagt ccgcaggaca tttatactaa gatgcgagct ggggattttg
    20161 aaatggctga ctttgaagcc catggcaaga cctacaaaaa cagctttatc acctatgaga
    20221 atttctacca aaatcatgag gatgctgagg ttcgtgagaa atccttccgt tccttctcag
    20281 agggacttcg taagcaccaa aatacggctg cagcagccta tctggctcag gtcaagtctg
    20341 aaaaactctt ggctgatatg aagggatacg actctgtctt tgactatctt ctagctgaac
    20401 aagaagtgga ccgtgtcatg tttgaccgcc agattgacct catcatgaag gactttgcac
    20461 cagtcgctca gagatacctc aagcatgttg ccaaggtaaa tggtcttgaa aagatgacct
    20521 ttgcagactg gaaattggac ttggacagcg ccctgaatcc tgaagtgact attgacgatg
    20581 cctatgattt ggtcatgaag tcggtagaac ctttggggca agaatattgt caggaagttg
    20641 ctcgttacca agaagagcgc tgggtggact ttgctgctaa cagtggcaag gattccggtg
    20701 gttatgcggc ggacccatat cgcgtacacc cttatgtcct catgagctgg acaggccgtt
    20761 tgagcgatgt ctataccttg attcatgaaa tcgggcattc tggtcaattc atcttttcag
    20821 acaatcatca aagttacttc aatgcccata tgtcgaccta ctatgttgaa gcaccgtcaa
    20881 ccttcaatga attgctactc agtgattact tagagaacca gtctaatgac ccacgtcaaa
    20941 aacgcttcgc tctggctcat cgcttgacag acacctactt ccataatttc atcacccacc
    21001 tcttggaagc cgccttccag cgtaaggtgt atacattgat tgaagaaggg gagacctttg
    21061 gagcaagcaa gctcaacagc attatgaagg aagttttgac ggatttctgg ggagatgcta
    21121 ttgaaattga tgatgatgca actctgactt ggatgcgcca agctcactac tacatgggct
    21181 tgtatagtta cacttactca gcaggactag tcatctcgac tgctggttac cttcatctaa
    21241 aacattctga aactggagct gaagactggc tcaatctcct taaatcaggt ggtagcaaga
    21301 caccgcttga gtcagccatg attatcggtg ctgatatttc aacagacaaa ccactccgtg
    21361 ataccatcca attcttgtct gacacagttg accagattgt cgcctatagt gctgagttgg
    21421 gagagtaagg gaatagaaaa attttttaga atgatgttta tgggcttgtt aagcgtcatt
    21481 ctatttgtct tagttgtagc tattttcaaa aatgtcgttg aaggtcgtga tgttaaattt
    21541 gaacttactg tagaactggt attgatagtt atttacgtca ttttatatca gattgtattt
    21601 aattgagaat tcaatattga gtcatttaag actgcctttt tgaacagttg ctagaaatgt
    21661 agaggtattg accatgtatc aagatttact aagaaaaata gcagaagaaa aaccaaatta
    21721 taaccaggaa gaaatccagt ggttgtttga tcatttggga aatccttctc cagaaattcg
    21781 taatgtcctt ttaaatcaag gcttgcacta tctttcaaaa gaaaaggata caacaggttt
    21841 ctcaagtcag tatggttggg ttcattcttt tgcacatgga gccgatttac tgacagaggt
    21901 ggtttgtcat ccagactttc ctataaacag agttcatgaa gtatttgata tacttggcca
    21961 actatttaaa agaatgtcga ttcgctttac agatgatgag gattggcgtt tagcaagagt
    22021 gatctatgaa cctattttac aagggaagtt ggagcaagaa caagtagctt cttggataaa
    22081 aactgttgat tttccgatag aagaaaggga ggatttttac aaattttcca actttagatc
    22141 ctgtctggtg gaagtctatg tccaacttga ccagagaaat agtttacaag atgagttgaa
    22201 agaagctatc cagtcttttc aatactagag attagctaat agttctatga ttttcaaaaa
    22261 atttccaatc aattttcttg aaaccctttc ctacttttga tagactagta tctagttttt
    22321 gaaaaaagga gaaagaaaat gaaaaaattt gttgctgagt taatcggtac gttcatgctt
    22381 gtgttcgttg ggacaggagc tgttgttttt ggaaatggcc ttggtcacct tggaatcgcc
    22441 tttgcctttg gtttggcaat cgtggtggca gcctactcaa tcggaactgt ttcaggtgct
    22501 cacttgaacc cggctgtttc gattgctatg tttgtaaaca aacgtttgtc atcttcagaa
    22561 cttgtaaact acatccttgg tcaggttgtt ggagctttca tcgcttctgg cgcagtcttc
    22621 ttcctcttgg gtaattcagg tatgtcaact gctagtcttg gtgaaaatgc cttggcaaac
    22681 ggtgtcactg tctttggtgg tttcttgttt gaagtcatcg caactttctt gtttgtcttg
    22741 gttatcatga ctgtgacttc agaaagcaag ggcaatggcg cgattgctgg tttggtaatc
    22801 ggtttgtcat tgatggcgat gattcttgtc ggattgaaga ttactggact ttcagtaaac
    22861 ccagctcgta gcttggcacc agctgtcttg gtaggcggcg cagcccttca acaagtttgg
    22921 attttcatcc ttgcaccaat cgctggtgga gttcttgcag cccttgttgc aaaaaatttc
    22981 cttggaacag aagaataatt gaaactcaaa aagccttgct cctcatcttg aggaacaggg
    23041 ctttttcgta tgatactctt cgaaaatctc ttcaaactac gtcagcttcc atctgcaacc
    23101 tccaaacagt gttttaagct gacttcgtca gttctatctg caacctcaaa acagtgtttt
    23161 aagctgactt cgtcagttct atctgcaacc tcaaaacagt gttttaagct gacttcgtca
    23221 gttctatctg caacctccaa acagtgtttt gatctgactt cgtcagttct atctgcaacc
    23281 tccaaacagt gttttgatct gacttcgtca gttctatctg ccacctcaaa acagtgtttt
    23341 gatctgactt cgtcagtttt atctacaacc tcaaacactg ctttgagtaa cctgcggcta
    23401 acttcctagt ttgctctttg attttcattg agtatgactt tagcggttgt caattttctc
    23461 tggataaagg tcgtgttgga agaggcgttg ttctgccaag ccctcatact tagttccttg
    23521 cttaccgtag ttatagtagg ggtcgattga aatgccaccg cgcggagtga attttcccca
    23581 ggcttctaaa tagcgagggt ctagcaagtt gaccaagtct ttcccgatgg tgttgataca
    23641 gttttcgtgg aaatctccgt ggtttcggta gctaaataga tatagtttga gggattttga
    23701 ctcgacacag agcttgtcag gaatgtagga aatatgaatc gtcgcaaagt ctggctgagc
    23761 agtgatttgt cccagcagag acatatcgag gatatggtga cgaatgccct gttccttagc
    23821 gatttctcta gtaatttgaa tttcgaggtg atgacgttgg ccgtaggcaa aggtgacagc
    23881 ttcgactgtt tcatagtgtt gcatgaccca gaaaaggcag gttgttgaat cttgaccacc
    23941 actaaagacg accaaggcta attgacgttt catagtactc cttccaaaat gggaaatgtt
    24001 cagagcacgc aaaaagctcc cattagggag ctaaaaaata ccaaatcgag gtttttttag
    24061 cgatggcata tcccaaacat cgtaatattc tacttatata gtaaaa
//
LOCUS       DAVKQL010000032        23134 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVKQL010000032 DAVKQL010000000
VERSION     DAVKQL010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23134
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.032"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(1..384)
                     /locus_tag="VW561_001220"
                     /pseudo
     CDS             complement(1..384)
                     /locus_tag="VW561_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(446..895)
                     /locus_tag="VW561_001221"
     CDS             complement(446..895)
                     /locus_tag="VW561_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU9943483.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(897..1655)
                     /locus_tag="VW561_001222"
     CDS             complement(897..1655)
                     /locus_tag="VW561_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU9943484.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(1770..3749)
                     /gene="pknB"
                     /locus_tag="VW561_001223"
     CDS             complement(1770..3749)
                     /gene="pknB"
                     /locus_tag="VW561_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEU9943485.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSAE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(3746..4486)
                     /locus_tag="VW561_001224"
     CDS             complement(3746..4486)
                     /locus_tag="VW561_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEU9943486.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDAE"
     gene            complement(4501..5814)
                     /gene="rsmB"
                     /locus_tag="VW561_001225"
     CDS             complement(4501..5814)
                     /gene="rsmB"
                     /locus_tag="VW561_001225"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEU9943487.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIANIKRKNKRDSIAYSLPVWLVTKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVADRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(5807..6742)
                     /locus_tag="VW561_001226"
     CDS             complement(5807..6742)
                     /locus_tag="VW561_001226"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU9943488.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGALFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(6755..9151)
                     /locus_tag="VW561_001227"
     CDS             complement(6755..9151)
                     /locus_tag="VW561_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEU9943489.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNPQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVVKRAYEVMNILRSSLSETSSILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(9217..9531)
                     /locus_tag="VW561_001228"
     CDS             complement(9217..9531)
                     /locus_tag="VW561_001228"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEU9943490.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(9556..10182)
                     /gene="gmk"
                     /locus_tag="VW561_001229"
     CDS             complement(9556..10182)
                     /gene="gmk"
                     /locus_tag="VW561_001229"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEU9943491.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(10312..11925)
                     /locus_tag="VW561_001230"
     CDS             complement(10312..11925)
                     /locus_tag="VW561_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEU9943492.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(12116..12370)
                     /locus_tag="VW561_001231"
     CDS             complement(12116..12370)
                     /locus_tag="VW561_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEU9943493.1"
                     /translation="MLLKFTEDAWADYCYWQNQDKKTLKRINKLIKDIQRDPFTGIGK
                     PEPLKYDYQGAWSRRIDAENRLIYMMDGDSVAFLSFKDHY"
     gene            complement(12374..12628)
                     /locus_tag="VW561_001232"
     CDS             complement(12374..12628)
                     /locus_tag="VW561_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEU9943494.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVHVIEE"
     gene            complement(12866..13963)
                     /locus_tag="VW561_001233"
     CDS             complement(12866..13963)
                     /locus_tag="VW561_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEU9943495.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVNILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGAEMEKFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLSYILVQARESNLPEAIHVLGFTEKGRQHLKSLKGQVSLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAEQNFGRVPIRIETN"
     gene            complement(13973..14719)
                     /locus_tag="VW561_001234"
     CDS             complement(13973..14719)
                     /locus_tag="VW561_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9943496.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFAMLWDTYEDEVPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            complement(14822..15175)
                     /gene="rsfS"
                     /locus_tag="VW561_001235"
     CDS             complement(14822..15175)
                     /gene="rsfS"
                     /locus_tag="VW561_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEU9943497.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            complement(15188..15688)
                     /locus_tag="VW561_001236"
     CDS             complement(15188..15688)
                     /locus_tag="VW561_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU9943498.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLGKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            complement(15689..16282)
                     /gene="yqeK"
                     /locus_tag="VW561_001237"
     CDS             complement(15689..16282)
                     /gene="yqeK"
                     /locus_tag="VW561_001237"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEU9943499.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLDLIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKEN"
     gene            complement(16282..16911)
                     /locus_tag="VW561_001238"
     CDS             complement(16282..16911)
                     /locus_tag="VW561_001238"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEU9943500.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRAFLAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            complement(16963..17274)
                     /gene="yhbY"
                     /locus_tag="VW561_001239"
     CDS             complement(16963..17274)
                     /gene="yhbY"
                     /locus_tag="VW561_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEU9943501.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(17489..18595)
                     /gene="yqeH"
                     /locus_tag="VW561_001240"
     CDS             complement(17489..18595)
                     /gene="yqeH"
                     /locus_tag="VW561_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEU9943502.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGIAKVAVWAPEGVAVVTRKAII"
     gene            complement(18598..19188)
                     /locus_tag="VW561_001241"
     CDS             complement(18598..19188)
                     /locus_tag="VW561_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEU9943503.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            complement(19140..20048)
                     /locus_tag="VW561_001242"
     CDS             complement(19140..20048)
                     /locus_tag="VW561_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU9943504.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(20105..20665)
                     /locus_tag="VW561_001243"
     CDS             complement(20105..20665)
                     /locus_tag="VW561_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEU9943505.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            20804..22015
                     /gene="sstT"
                     /locus_tag="VW561_001244"
                     /pseudo
     CDS             20804..22015
                     /gene="sstT"
                     /locus_tag="VW561_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            22083..23036
                     /locus_tag="VW561_001245"
     CDS             22083..23036
                     /locus_tag="VW561_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU9943506.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
BASE COUNT         6200 a         5461 c         4551 g         6922 t
ORIGIN      
        1 ccttgataac cactgtcagc caagatttta ccagcttgtc cgatatttct gcaactcatt
       61 ttgaacaact tcatatcatg acaatagttc acagtgatat ccaaagaaac aattctccct
      121 tgacttgtga caatcgcttg agtcttcata gcgtgaaatt tctttttacc agaatcattc
      181 gctaattctt tttttagggc gattgatttt tactttcgtc gcatcaatca ttaccgtgtc
      241 ctcagaactg agaggagttc ttgaaatcgt aacaccactt tgaacaagag ttacttcaac
      301 ccattggctc cgacggagta agttgctttc gtggatacca aaatcagccg caatttcttc
      361 ataagttcga tattctcgca catatagaaa gcgttgttaa tttatttatc tcatttttca
      421 gaaaattctt ttatttctgt aaagtctacg atactcgatg tgtttttata taatgataca
      481 gtctgagaat cactgttccg ctagccattc caatagagat taccagagcc aacatgacaa
      541 ccaaggtcgc acttgccagt gctttattat agtcccctgt cacaaaaaag gcagttgttc
      601 ggtaggagag ataacctgga accagcggtg ccaaaatggc caagataaag accacagcag
      661 gtgtcttata aagaatactt aaaatctggc tgacacaaga accaataatg gctgcaatga
      721 aggtagctac aatgacattg gtcggttcct tgagcaagag atagattagc cagacagtca
      781 tgcccaaaat ccctccaggc aagagcatag accgttgcac attgagtacg attaaaaaag
      841 tgataatggc aagaaaactt gctactgctt gtaataaaaa ggttgttagt gtcatattag
      901 ttcatcaata ccaaggcgac agaagttcct gcccctaaag cgagggtaat gagcagggat
      961 tcaaacatct tactcatacc agagtttatg tggttggtca taatatcacg gaccgcattg
     1021 gtcaaggcaa tacctggtac aaacggcatg accgcaccag ctataatcaa atctgccgtt
     1081 gaaggaaaac ctgtgtagcg agcccaaaac tgggcaatta tcccaaagac aaaggctcca
     1141 gcaaaggctg tcacaaaggg aattcggata aatttttcca catagaggga aaaggcaaaa
     1201 ccaaataagg tcgccactcc tgccccaagt gcgtcgtaga tatttccgct aaacataact
     1261 gaaaagaaag gagcactaaa ggtcgcagcc agagttacct gcaacttagt atagggaagg
     1321 ggttgagctt gcaaggccgt caattgctta aaggctgttt ctaagtcaat ctgcccccca
     1381 actagctgac gagaaatctg gttcacatcg cagacttttt cgatgttata agaagaggag
     1441 gtcacgcgct tcatgcgcga aatattggta ttttcaatag agaaaaagat agcggcaggc
     1501 atggcaagga cattgcaatc cacaatcccc tgcgaatgcg cgattcgaat catggtatct
     1561 tctacacgat ggatttctga gccactttta aggagaatag tccccgctag cataatcaca
     1621 tcaatgacgg catttaattc ttttgattct tccatgcttt cctcctttta tcaactccct
     1681 ctattctatc acaaatctgg actcaaaaaa aatctttgcc atgaaatcat gacaaagatt
     1741 gattactcat tttgattatc catctgcttt taaggagtag ctgaagttgt tttaggtttg
     1801 tagattgaaa tcttgactct agtcttattt aggtctacct tttcacctgc tctaggactt
     1861 tgttcaacaa ccatgccttc tgcactacct gcaggcgctg tcgtcacttc tacaacttct
     1921 atattagctt ccttaatccc aacaatttga atcaaattgt tcttagtaaa ctccaagcta
     1981 gaaccaatgt aactcggcat ggcaacactt gtaacttttt tagctactgt caagacaatt
     2041 tgagtaggtt tactcacatc ataagtcgtt ccggcacctg gactttgttt cataatcgtt
     2101 cctggttcgc tttcgctgga ctcttcttcc tctatcttaa tcaaattctc aggaaccttc
     2161 ttctgcttga gttctgagat tacttctgta gagttccgtc caatatagtt ccctaattga
     2221 atcgtcgtag cttttttagc tactgtcaaa acaatttgag ttgccttgct caagtcatag
     2281 gtcgtacctt ctggtagact ttgcttcagg accgttccag cctcactctc attcgactct
     2341 tcttcctcaa ttttaatcaa attatctgga acttttttct cttttaattc cgcaatgaca
     2401 tcagaggatt tccgaccgac ataattacta atttggaaag attgcttgcc tgatgagaca
     2461 accaaattga ttttcgttcc ttcttttcga ccagttccag cgccaggatc tgtacggata
     2521 atccgccctt cttccacctt ttcactagcc tctgtcttct cctcaccaat ctcaaaattg
     2581 gcttttttga gcgttgcctt ggcctctgca actgtctgac ctgccacatc tggaatggca
     2641 atggttgcag gagttctgga taatatccaa ataagagaag ctgccaccaa tacaaggctg
     2701 gccaacaaaa tcaggtaacg catcttaaat ctatgttttt tcggtgcttg tggttggtaa
     2761 gtttcctctg tcacagcctg gcttgggttt ttgattgatt tgtgttctgt ctgcgcttga
     2821 accttaggaa tagatgtcaa ggtactctga gaaaccttcg gcaaggtctt ggtatctgcc
     2881 ttgctcgttt catcaaagat taacttactt tcatttctac gattgtagga caagctacta
     2941 gacaagtcca catacatctc tgaaaccgag cggtagcgat tggtcaactt tttagcagtt
     3001 gccttgataa taacattttc taaagcctga ggtacagatg gattttctgc aataacggac
     3061 ggcaggggtt tctggaaatg ctggagggca atggtcaccg cgctatcccc gtcataaggg
     3121 atatggcctg tcaacatctc atagaaaata atccccatgg catagatatc actctgcaca
     3181 gtcgccttcg aaccacgcgc ctgctctggt gacaagtaat gaactgagcc caacatcgag
     3241 ttagtctggg tcagacttgt ctctgcaaag gctacagcaa tcccaaagtc tgtgaccttg
     3301 gcagtcccat ctggtgtcaa aaggatattt tgaggtttca agtccctgtg aacaattcct
     3361 cgagtatggg ccaagcgcat agccaagaga atttgtccca tgatacggac ggcttcttca
     3421 ttagaaagag gataatgttc cttgatatag cgtttgaggt ctagtccagc aacatactcc
     3481 attgcaagat actgttgacc gtcttcctca ccaatatctg ttatccgaac gatatgagga
     3541 tggtctagat ctgccatagc tctcgcttca cgctgaaaac gagctacagc tatcgggtcc
     3601 gtctggtagt tggtcctcag aaccttcact gccacttctt ccccatctaa gattaagtct
     3661 ttggctaggt agacatccgc catgcctcct cgaccaatct gtttgacaat ccgatagcgt
     3721 ccggcaaaaa tcttgccgat ttggatcatt ctgcatcctc ctcgttcata gaaacaaggg
     3781 caaccgtaat gttgtctaaa cctcctgcat tgttagcaaa acgaacaagt gtctccgttt
     3841 tatctgctaa aggaatatca ctggttacaa tatcacgaat ctcactgcct gaaatcatgt
     3901 tggtcaagcc gtcactattg agcaagagat agtcacctga ctcaaggata actgtcccaa
     3961 aatcaggctg aatttcatct ttttgcccaa tagactgggt gataatattt ttttgcggat
     4021 gagcttctgc ctcttctggt gtcaattgac cagccttgag caattcatta accaaggaat
     4081 gatcgctcgt caactgatgg tattcttctc cacgaatcaa gccgatacgc gaatcaccaa
     4141 tatgagcata gatagcctga ttatcaataa tagcaaggac ttccaaagta gttcccatgc
     4201 ctctgtaagc ttcatcctga ccaagctggt gaatcttttg attttcaatt tctaggtaat
     4261 gggcgaacca ttcacgcact tcattgactg tatcgatctg ggtatcaacc caagctacac
     4321 ccaggtctgt caccgccatt tcactagcga tattccctgc gcgatgacct cccatcccat
     4381 cagctaaaat aatcatggta cgtccagctc tattgacata gtggttgaca tagtcttggt
     4441 tatttgttcg tttctgacca acatctgtta ataatgaaat ttccatgtgt cagttccttc
     4501 ctaatccgat atcttgcgaa attgactgat gaagaatcca tcacttccat acaattcagg
     4561 tgtaatgagg atacagccgt ctttcatgat atccttacat tcatgttcta gttttacctg
     4621 ctcgaactcg ggatgactct ctaaaaaggc cttaacgact tgaaaattct cctctgagac
     4681 gatagtgcag gtgctataag ttattatacc acctttgcct agtatttgac aaacactacc
     4741 taatatttct aactgaattt cctgcaagga cgcgaaatct gccgtttctt tattgtattt
     4801 gatatctggt tttcggcgca aaagaccgat tcctgaacaa ggagcatcca ccaaaatctt
     4861 atcaaaggaa tcctggtcaa aaaactcatg cacctttctg gcatccaatt tttgagtttg
     4921 aacccgatct gcaactccca gacgttgggc attttcttga attaaatcca acttgtggtc
     4981 gtacaagtcc agagcagtaa cctgacctgt cgtaagataa gaggctatat gggctgtttt
     5041 cccacctgga gccgcacagg catcaagcac tcgctcatca ccttgtaaat caagcgtcgg
     5101 agcaaccagc tgactggact cgtcttggat ggtaatggct ccatccgcaa acaaattatg
     5161 ccctgcaaaa tgcccctgct ccttaaccag accagtggtt gctaaaaggg aattattcgc
     5221 ctccaacaag gcttggattt cctcttttcg acttaggtct gttacacgaa tactagcttt
     5281 gtttcgcact aacaggcttt caaagatggc ttttgctctc tcctctccgt attcttcctt
     5341 gagtttggta actagccaaa ctgggagaga ataggcaatg gagtcacgct tgttttttcg
     5401 cttgatgtta gcaatatctg gccagccttc acgcaagata cggcgaagga cagcgttgac
     5461 caatttttca ctgccttttt tacggagttt ggccaattcc actgcttcat tgaccacagc
     5521 atgatctgga atcttgtcca aatagcggag ttggtaggca ctcatgagaa gaaggacata
     5581 gagccagctg tctaactggt ctctgtcttc gataaagtgg gataggtacc attccagagt
     5641 cagtttacgg gctaccgttc catagactag ctcggtcact aagcccttgt ctgctgcaga
     5701 gagctgactc cccttgaggt gtttatttaa ggcgatattt gaatatgctt ggttcacaaa
     5761 aacatcctct agcactgcta gagctaaact tctagccgtt tctactttag tcaccaaatc
     5821 gttctcctac agtcaatgta cgtccaactc cgttgaggaa ggaagcaatg tccatcttag
     5881 gcttaccagc tggctgcact tgtttgaggg atagagcccc ttcagccgtt gcgacaatca
     5941 attctttctt gccgatagag agaatctcac ctggatttcc ctgaccttct actggtaggg
     6001 cttcataaat cttaaagcgg tcgcccttaa ggaaagtatg ggcaacaggc caggggttca
     6061 ttccacgaat ttggttaaag agttgacgat tggttttgtt ccagtccagt ttttcttcct
     6121 ctggctttat atttggagag aaggtaacct gactcgtatc ctgcggttca ggtttgatat
     6181 caccagcaat ataggcaggc agagtgtcca aaagcaaatc acgaccaact agcgccaatt
     6241 tttcaaacaa ggcgccaaca ttgtcctcat ctgtgatcgg aatgctgcga cgagaaatca
     6301 tatctcctgc atccatttcc ttaaccattt ccatgatggt cacaccagct tcctcatccc
     6361 cttgaatcaa ggcataatgg ataggcgcac caccacggtg tctaggaagg agggaggcat
     6421 gaacgttgac agcaaagtcc atgctatcaa ggagtttgct tgggagaaac tgcccaaaag
     6481 cagcagtcac aattccatct gctcctagct tcataagatc ttccatctct ggacttccag
     6541 ataatttttc aggttggtag atagatagtc ctgcttcctt ggcagcctgc ttgactgggg
     6601 tttcttggat aactttttta cgaccaacag cacggtctgg ctgggtcaca acggctagaa
     6661 tttcgtaacg gtcatctgtc aaaagtcctt ttaagactgt tgctgaaaag tcgggggtcc
     6721 ccataaagat tagttttgtc atatcttctc cttcttataa aaattgctgc ggctcatggt
     6781 caatgctgag acggagctca ctattttccc gttcttgagt caaggccagg acctggttga
     6841 gggtcggccc cagctcatct tctaaacggt atttaattaa aatctggtaa tgatagaggt
     6901 tgtgggtacg ggcaatcggt tttggcgttg gccccagaat ggaactggtc tctgacaagc
     6961 ttgaccgcaa aatattcatg acttcatagg cacgtttgac cacctcttct tctttcttgt
     7021 gagaaagggt aataccaatc gtgaaatagt aaggtggata gccgagttgt cgtctgattc
     7081 ccatttcata ggcataaaag ccttcgtaat cttgatcctt ggcaaatcga atagcatagt
     7141 gctgcggatt gtaggactgt atcaagactt gacctgcctt ttcagcccga cctgctcgac
     7201 ctgccacctg agtcaagagc tggaaggttc tctcagaaga acggaaatca ggcagattca
     7261 aggccgtatc cgcatttaga actccgacta gggtaacatt gggaaaatcc aacccctttg
     7321 caatcatctg agtaccaagt aaaatatccg cttcccctcg cccaaactgg tcaagcaagg
     7381 cttggtgact gcctttcttt cgagtcgtat ccacatccat cctcagaatg cgagcttggg
     7441 gaaagagttc tgctagctca tcataagcct tctgagttcc cgtcccatag taacgaatac
     7501 tgcggctctt acagttagga cagacctgag gaatatcctt cgagaaacca caataatggc
     7561 agttcatagt cttggtatcc atatgcaagg tcagagaaat atcgcagttg ggacaagtat
     7621 ccaccgtccc acactcccga cacatgacaa agctagaata accacggcga ttgagcatga
     7681 gaaccacctg ctctttttta accagacggt cttggatagc ctctagcaaa ggaggcgtaa
     7741 agtttgacgt ctcattttgt ccgatatagt ctcgaaagtc aatcacttga acctcaggga
     7801 ttgtagccaa aggattggca cgttgggtta gacgtaagtg ttgatagacg cctttgccag
     7861 cacgtgcccg gctctctaag ctcggcgttg cagatccaag taccagagtt gcttgattat
     7921 actgagcccg taaaatagct acctctctgg catggtaacg gggattgctg tcctgcttat
     7981 aagccgcttc atgctcttca tcaataatca tgacacccag atttttcaga ggagcaaaga
     8041 tagcagatct ggcaccaaca acaacttggg catcgccacg ctccaccttg cgccattcat
     8101 catacttttc accattggat aatcctgagt gaagaatggc taccttgtcc ccaaaacgtg
     8161 ctataaaacg ctcggtcatc tgaggagtca aggaaatctc aggtaccagc aaaatagctg
     8221 tcttgccctt atccagggca ccttggataa tctgcaagta aacctcggtc ttcccacttc
     8281 ctgtaatccc ttgaagtaga aagggaggtt gagaactgcc aatagaactc acaaccgcat
     8341 cacgcgcctg tctttgttct ggatttaact ccaaaggtct acttgcttca attccttcaa
     8401 aataagcagc cgagcgttga acttcctttt ggactatggt aacagcacct tgatccacaa
     8461 agaagttgac ttgctctcgc gagtaggact ctaacaagct agccaaggaa gcgctctctg
     8521 gatgagacag cagataatct ctcagttcca actttttctt ggcacgtgta gaaatctcaa
     8581 caccttctaa ttgagcatgg tcaacctcat accaagactg ggtcttgacc ttcttttgat
     8641 cgactgcctg atattccaga ccaagcaggc cttttctagt caaacgcatc atttcagctt
     8701 gcttggcaag gtctagtgaa gaaaaggcta gcgaatcttc tgaaccaaac aggcgcactc
     8761 gttcttcctg actcaagcct tccagaggat agagaatctt gtcatagcta gaattcagaa
     8821 atcctggaag catggccttg aggatagaaa ttttgtagga gaagacagat ttgcgtaact
     8881 cctcagccag ccagagttgt tctggcgtga gaacaggaga aaaatccagc acctctgcaa
     8941 tatcttttaa atcttgctcc atctcttctc catctgattg ggactccaaa ccaagaacaa
     9001 tcccttgaat caggcgatta cccttaccaa aaggcacatg aacccgcatc ccaacttcca
     9061 gcattccctc aaattcctcc ggaatcctgt aactataggg ctggtccgtc tgcatcaagg
     9121 gcacatctac gataatctta gctagggcca tcttctcacc tcctccttgt cagtacattc
     9181 ttgcaataga aaaaataaga ttgagtcccc ccaaccttaa attttttcac catcttcttt
     9241 ttctttagca atttgctctt tgattttctt ttcttcttct tctttgcggc gtttttcttc
     9301 ttcgatacgg cgacgcactg cttcacgttt tccttctgga tctgggtgaa ttgtaacgtt
     9361 tcctgattcg atttcttcta aagcgcgaag agttgatttt tcagacttga aaccttgagt
     9421 tgctggggca cctgcttcca attcgtgggc acgttttgct tccaagatta cgagtgaata
     9481 ttttgaagga accttgtcga gcaaggtatc aatagagggt tttaacatca tttgcttgta
     9541 cctattttct aaattttatc gggtagttgg agattttggt aacatctcct gatagtgacc
     9601 aatgacacga tccacacaga agtgttctgc ttcaatcaca catttgacac gttcagcagc
     9661 taggggcacc tgatcgttga caatcgcata atcatactca cgcatgaggg caatttcttc
     9721 cttggccttt tcgattcgtt gggcaatcac ttctgcacta tctgttccac gacctaccaa
     9781 gcgatcttgc aattcatcca aatctggtgg tgtcaggaag ataaagacag catctggaac
     9841 ctttttcttg acctgaagag caccctgaac ttcaatttca aggaaaacat cgattccctt
     9901 gtccaaggtt tcattgacat aggtcagagg agttccatag tagttgccga catattctgc
     9961 gtattccaac atctgtcctt gacgaatcag ctcttcaaat tcttcacgag tacggaagaa
    10021 atagtcaaca ccgtccactt ctccaggacg ttgtgcgcgt gtcgtcatcg atacagaata
    10081 ttgaaattgg ttttcagaac tctcaaaaat ctctcttcta accgttcctt ttccaacccc
    10141 tgaaggacca gaaaaaacga ttagtaagcc tcggtctgcc attgtgtctc cttttagtca
    10201 atctgtgaaa taacatttct ctagaataat ggcaaaaagc cagattatcc tttacagtct
    10261 ttctatctag tgtaacaaaa aagcagtaat ttttcaactg ctctttctta tttatttagc
    10321 ataatctact gcacgaagct cgcgaatcac ggttaccttg atatttcctg gataatcgag
    10381 attgttttca attttcttac gaactttgtg agccaagatt gtgactttgt cgtccttgat
    10441 ttttcctgga ttgaccatga tacgaatttc acgtcctgct tgaagggcaa agctagtttg
    10501 cactccttca aagccgttag caatttcttc caaatcatgg agacgcttga tgtagctttc
    10561 aagagactca ctacgagcac ctggacgggc tgcgctcaag gcatctgctg cagcgacgat
    10621 aactgctatc acgctctcag cttcaacatc tccgtggtga ctagcaatcg tattcaccac
    10681 aactgggggt tccttgtact tacgggccaa ttccataccg atttcaacgt ggctaccttc
    10741 aacctcatgg tcaatggctt tcccgatatc gtgaaggaat ccagcacgac gggcaagagc
    10801 cgcattttca ccaagttcgc tcgccatgat accagccaac ttagcaacct caatcgaatg
    10861 gcgcaaaaca ttttgtccat atgaagtacg gaactgcaaa cgtcccataa tcttcatcaa
    10921 gtctggatga aggtttggcg caccaatttc ataggcagca gcctcaccgt attcacgaat
    10981 cttattgtca atctcttgac ggtttttctc aaccaactct tcgatacgag ctggatgtat
    11041 acgaccatct ttgagcaaca tttccatagt catacgggca atctcacgac gaatcggatc
    11101 aaatcctgac aaggtcacca cttctggtgt atcgtcgata atcacatcga cccctgtcaa
    11161 actttcaaag gtacgaatgt tacgaccttc acgaccaata atgcgtccct tcatagtatc
    11221 gtctggcaga tgaactgttg agtttgttga ctccgctaca tattcaccag cgatacgttg
    11281 catagcttga accaagatgt ccttggccat tttgtcagaa cgttccttga cctcttgctc
    11341 agcttcgcga atgcgactgg caatctccct ggtcaagttt tcctctgtct gagccaagat
    11401 aatatctcgt gcttctgcct gagacagagc accaatacgc tctagttctg cttctttttg
    11461 tctttcgact tcctctaatt gctcttcacg cgcatcaagg tttttcgctc tatcagaaat
    11521 actttgttct ttttgttcaa gtgtttgttc tttactcgtc aaattgtcgt ccttacggtc
    11581 aaggctagta gctctctctg tcaaacgact ttcgatttgt ttgagttctt gacgttctga
    11641 tttgaattca gcgtccactt cttcacggta ttttctggct tcttctttgg cctccaatag
    11701 tgcttctttt ttaagagact tgctttcacg tttggcttca ttaacaagta aatccgcttc
    11761 acgctcagct tgtccacgta aattagttgc ttcttgttca gcatttaaaa gcatcaactc
    11821 tgcagcttcc tgagatgatt tcatcttagc tgagatgctg acatatccaa tgactaaacc
    11881 aatgatgacg gcaaaaacag caatcgcaag cgacatgatt tccatgtttt tacctcattt
    11941 tattgttatt ccgaatgaca tacattcttt tacattctac cataaaaaag tgattttcac
    12001 aaacctaaaa tagaatatgt tttggggaat ttggaacaca tttgcaaaaa taaacttgtt
    12061 gtttagaaat agtagtttag tagagacttg agaaaaagcc tactttacag tagacttagt
    12121 aatgatcttt aaaggacaag aaagccacgc tatctccatc catcatataa atcaagcgat
    12181 tttctgcatc aatacgccgt gaccaggctc cttggtaatc atatttgagt ggctctggtt
    12241 ttcctattcc tgtaaatgga tcgcgttgaa tatccttgat taatttattg attcttttta
    12301 gtgttttttt atcttggttt tgccagtagc aataatctgc ccaggcatct tctgtaaact
    12361 tgagtagcat tcatcactcc tcaatcacat ggacctgagt gcttccagca cggacttgag
    12421 ccattcctcg taaaaccttg tctgataact ccttattttg ggcgattctc agggtttcct
    12481 gaatactgtc ccattcgctc tttgaaagaa ctacaatgtc ctcatctgga tttttattga
    12541 ccaccgtcaa aggctcaaat tcatcattta ccttcttcat gtagtccttt aaatgatttc
    12601 ggaatgttga gtaaaggact gcttccataa ccatacctcg ttttagctct tttccactat
    12661 tatacacgaa aagaaagaaa ttgtcaggaa cttgtacaag attttctttt ctatctattt
    12721 atactcaatg aaaatcaaag agcaaactag gaaactagcc gcaggctgta cttgagtacg
    12781 gcaaagcgac gttgacgcga tttgaatttg attttcgaag agtattattc gtaaaaaatc
    12841 tcaaaaagcc tacctttcag tagacttagt ttgtttctat tctaattggc actctgccaa
    12901 aattttgctc tgctatactt ggctttccta gttggtaaat ctggtcagcc ttttgagtca
    12961 tagcatccca aggttctttg ccaattcggc tgactagact gacctgacct ttcaaagact
    13021 tgagatgttg cctgcctttt tcagtaaatc caaggacatg aatggcttct ggcaagttac
    13081 tttctctagc ctgcaccaag atataggaca agaggcgtct gacacgcgcc ttggtgtaac
    13141 gtttggtagt cactaactcg accaattctt ccacagactg ggctgtttta atagcttcct
    13201 taatgcgcac agccatttct tgattgacct gatagatggt ggttaggtcg ggatttgaca
    13261 agatttgata gcggagcaag ggaaaatagt cttcccagat caccttactg gcctgctcaa
    13321 atagggcaac agaaggcata aaacgttcta agaaatcttg gtccttctga tgttgacgga
    13381 gggctgtcgc cgaggcaaag tccacatctt tattcacaga atggtaacct gccccctgac
    13441 gctgaatcgg atgcagcttg atatttcgtc ctgcaaccgc cttggcatag gccagagcaa
    13501 gaacatgatt gggggtatta cctgaaaaat caagaccagc aaattccttc cacatagctt
    13561 gggttttctg gggataggaa agggaatcag gcagattttc cacaaatttc tccatctcag
    13621 cacctttctc tgtgtataag tcagcgattt tctgataatc tcgaacttcc tctgttccaa
    13681 aaactagact atcaatgccc aagcgatcca agatattcac agctccttga ccaaagaaat
    13741 cagctgcctg aacactgact aaaaagggca attccactac caagtctgcg ccattttcca
    13801 gcgccatctg ggcccttgtc cacttatcca caatagcagg ctctccacgc tgcatgaaat
    13861 tccctgacat ggcaacaatt ttcagtccct ctgcctgatc cagcaggtat ttatggccat
    13921 tatgaaaagg attgaactct gcgataatac ctgtgatggt catgatgttc tcctacttct
    13981 gcgccacaaa aaaccaacgg gtgctagttt ctgttggctc cttatcctca aagtctgcat
    14041 agagtttgaa ggacttaaat ccagcctgtt ccagcaagat atcataggtc aagatctcat
    14101 aggtcctctc ctcatgtact tcatcgtggc gactaaagga accgtcagcc tccttgataa
    14161 agaaagtcag ctcatgcacg atggagtgag gaacttcgtc ctcatatgta tcccaaagca
    14221 tggcaaaatc ttccgcattt tcatggtagg aatagccagg gaatacttca tctgtctggt
    14281 aggtcgagtg cacgtcaaag atgaaaactc cctcttcatt cagcgcattg tacacgtcct
    14341 taaagacgtc ccctacttcc acctcatcct gcatatagca gatagagtcc gaataacacg
    14401 tgacaaaatc gtatttccct gccttggaca aattcagcat attaccttct ataaaagcaa
    14461 tcttttgctt ggctgaagtt gctctcttct ccgcaatctt caacatatcc gcactcaagt
    14521 caagtccagt cacatcaaaa ccagcctgag agaagcgcac tgattgaatt cctgtcccac
    14581 aagccaattc caagagtttc tttctctcct tggtcttagg caaatgacgc agagaaaaat
    14641 tcgtccattt gtcgtataaa ctatcgtcca tgaccgcatc atagaccgcc gcaaaggttt
    14701 cataagtcgc cataatcatg atactaagag ctccatggta aacaccacta gccctttacc
    14761 tttctatcaa tttttctaaa ataagcaaag aaacccagtt gactacaact gagttcatct
    14821 tctaagcaag agcttctgaa atatctactg aattcgcctc atgccatagc ttctctagat
    14881 tataatgggc acgcatttct tctgaaaaga tatgcacgac aacagcacca aggtccagta
    14941 agacccagcc tccagctgca tcgccttcga catggctacc tttaaagcct gcttgagcta
    15001 ctttttcacg gatattagct gcgatagcgt ccaactgacg gctattcatt gagctagtga
    15061 tgacaaagta gtccgtcaca ctagtcaaat cttgtacatc aagtgcgagg atatcctccg
    15121 cacgtttctc atcagccgct ttcacgacta gttctagtaa ttctttttcg ttcatttaat
    15181 cctcttttta gaaaatatgt ttgtaatcta gaacatctgc aaagcgctcc tcccaaatgt
    15241 cctcataaaa ttcattaatc gtttctgctg gaatgtcatc cccatcaaag gttgtgacag
    15301 caccttctgg aacaataagc tgatagccat attcaaaggc aaccttgaca gaggtatcca
    15361 cacaatattc tgtctgcata ccacataaaa ctaatttttc aatcccctgc ttatccaagt
    15421 attcttttaa gccagtttct ttgaaaatac tgttatattt cttctgaaag accttttcag
    15481 caggttttcg atttaaaagc gcagataact gccaatcttc tgatgtttga gcttcagagt
    15541 tctcaatatg ttgaacatag ataatttcaa tattcttgct tctagcctgg ttttgtaaat
    15601 aagaaatttt tcccaataga ctttttgtct ggaatccagt ttctactaaa acattctgaa
    15661 catcaatgat tataaaagcc gtcttcattt agttctcttt caaatagtgc acaaaggcgt
    15721 tataggtttc aagggtttgg ggatagatgg ggaatccctg atgagctaga tactccacgg
    15781 tacgagctgt ttcgtaggcc accgccttat tgagcgatag acttgcaatc tcacgcgcca
    15841 catctactcc aggaaaggct cgattgtgct cgatatagtc tgcgacgtag atgaccttat
    15901 ctaggtctgt catttgacca gcaccgactg tatggacttc aatggctcgc aggatttctg
    15961 aatcatgcaa atccaaatct tcctgaattt tgtaaattcc gaccatacca tgccagacat
    16021 tattgcccca gtttttgagg tcagggtcta gctggtaacg gtcaatcaag tctagaaatt
    16081 cctgatctga caacttttta gcatagtcat gaagaagacc tgctagactt gctttctcaa
    16141 catctacacc aaagcgctga gctaattcca tagcggcacg ctccacacct agacaatggg
    16201 ttagacgttt ctcaggtaga agctctgcca ttttttccaa caaggcctca cgggagcagt
    16261 tgatatagtc ttgataggac atcagtagag cccctccttc tcgatgtagt ctagcactgg
    16321 ctgaggtagg agaaagttgg gtttccgacc ttgggcaagg aaggcacgca ccatgctgga
    16381 cgagatatcc atgagcggta cgtccaccca gataactgga taggaagtcc ctgccttgta
    16441 gcgtggacgc tgaaccccca caaactgaac catgtcaacc agttcatcaa ttcggtacca
    16501 cttaggcaga tagtcaacca tgtcggcacc gatgataaag taataatccg tatctggatt
    16561 cttctctgtc aaaatcttca tggtgtcgta ggtgtaggaa atacccttac gctccaactc
    16621 aatggtttca atgactaggc cgtcaattcc ctcaattgcc aactcaagca tcttgagacg
    16681 atggtgttca gggatggttt cctttttatc aacgtgagga ggttggtatt caggcatgag
    16741 aagaacttga tccagtccca actgttgccg tacttgatcc gcaacaatga gatgggcatt
    16801 gtgaacaggg ttaaaattcc cccctaaaat cccaacttgt ttgcgttttt tctccttgat
    16861 ttctggctcc aactctacct tggtaaaggg agtcaatagt tcgattgtca taggctagtc
    16921 ccctttattt ctctataaaa acagttattt ggagtttcgg tcttagattt ctttgacttt
    16981 cttagaaatc ttgcgatttt ctttcttgct agattgttta aacaaaatca agatgcgtcc
    17041 tattttttgg actgtatcca caccgatttc ttcttccaaa atttcagcta cttcgtggat
    17101 gttttcatct gtgttttgta agagagtaac cttgattaat tcacgcgcat caagagcttg
    17161 acggacgctg gttttgattt ggtcgttgag tccatttttc ccgatttgga tgataggttt
    17221 gagggtgtgt gcctggctgt tgaggaaggc acgttgtttt gatgttaatg acataatttc
    17281 tttaaaagag tttcttttta tacttttctg aagtggtgac ggacgtcagc aaagtccttc
    17341 ggactttcat gactaaaatt tgagcctaag gtctcaaatt ttccgcagtt ggtacaatca
    17401 cttgtaccaa cttacaccac agcgaaaagt attctctatg gggctcgcct tgctcgccca
    17461 aattcagaca accctttcct ttctgtgctt aaataattgc ttttcgtgtg acgacggcga
    17521 cgccttctgg tgcccagacg gcgacttttg ctatgcctgt tacacgaatc catcctaggc
    17581 ctgagatgac taggtctgtc ttatctttaa tggtaaagac atgctggact agccttggga
    17641 aatcttcttt ttccttgcta tttggtggtg tcagaagagt tcccaggtgc ttatcgtaga
    17701 aagcactagc tccttcaagc ttgctacgat ggagtttgag ttcattatca aagaaagcag
    17761 taaatccttg cttttctcct gctataaagt caaagcgtcc caaaccacct aaaaataggg
    17821 tttgctcagg attaagctga taggtcttag gcttgatttc ctttttagga ctgacatact
    17881 tgaggttttt ggccgtcaag tagtgagcca tctggtggcg gtggataatt cccggcgtat
    17941 cgtaaatata agatccgtcg tcaagcggaa tctctatttt gtccaaggtt gtccctggga
    18001 agcgtgaagt agtgatgaca ttctgatcac ccgtgatttc ttggataata gcattgatta
    18061 gagttgattt tccaacgttg gtcacaccga ccacatagac atcgcggccc ttacggtagt
    18121 gttcaatctt gtcaatgact tccttaatgg catgtttatt ttgtgctgaa gttaggacca
    18181 catcgactgg acgaagacct tcttcatggg cacgtttcat gagccactgg ctaatcttac
    18241 cagacttaac tgacttagga aggatatctt ttttatttcc taccaagagg acatcattgc
    18301 ccgagacgaa acgtggtaaa cctgggatga cagatccatt aaaatcaaag atatcaatga
    18361 cattgaccac taaagcatca ctgtctccca cctcgtgcaa gagcttgagg aaatcatcgt
    18421 tcgtcaactg gacatctgtg atttcattgt agtggcggag acggaaacag cgttggcaat
    18481 agacttcgcc agtctccaaa cctttttcaa gtgccgactg gggggtaaaa ccaagaccag
    18541 ctttatctgt cgtctgaatg gttgctccac aaccaataca gagaatttct tccatagtta
    18601 aattcctttt ttatatgtaa tcggtccgta cttttcagtg atttttctca taacacgacg
    18661 ctcacgagtt cggttaatct gcgttttgat tgagtcatgt tggaccaagg gtttgactaa
    18721 aattgaccga atccctgcac ggtgggctgc tcgtatatct gtcatgagct ggtcaccaac
    18781 catgaccact tcctttttgt catagtggaa ttccttcata gcacggtcaa taccaaatgt
    18841 gaagggcttc aaggcccagt aaacgtaatc aatcccaaat ttctcaactg ctcgttgaac
    18901 gcgttttttg gtgttatttg acactacgat aatgccaata cccgcgtccc gaaggtcatg
    18961 tagccattgc ttcatctctg gcgttccatc agggttgttc caagcaatga gggtattatc
    19021 caaatcgacc aaaacagcct tgattccctg cgcctgcagg cttgggactg tcagatcata
    19081 gactgcttcc acagcaaaat ctggtatata attttcaatc gccattctgg ctccttttct
    19141 taactttttt tcgcaatttt ccttagtaac aaggataaaa caatccacaa acctgcacta
    19201 gccaaactga tatagagcca agcatggggc tcatctgtta aaggtaaggg aacattcatt
    19261 ccgaaaaagc ctgtcacgac tgccaaaaca gctagcaaga ctgaaatgat agtcaaggtt
    19321 gtcaaattgt cattcagatt attgtttaga atattgttgt aagaggctga aagctgctgt
    19381 aaaatctgag agattaggtc tgtcatggat accagctgat gagcctcaat catggcatca
    19441 tcaaactgtt ctctctcaat ctcatcaaaa ctacgataca aggcatgacc ttgaatatgc
    19501 tctaacaaaa tccgattttg tttggcagct gccgtcagat aaaccatacc agtctccaaa
    19561 tcagacaggg caaagaggtt tttcttggta gttcgctggc gcaagaggtc attgacctca
    19621 tccctactct tgtccatctg ctcaatgaca ggatagtagg cattgctgat aatttccaga
    19681 ctggcaaaga gaaacttata aatcgaaagc gtgtcatggc tctccagata acgagtcatc
    19741 tgttcaatga cataggcgtt cttggtatta ctaatggtaa tcaggcgacg atgctcgaca
    19801 ataaaggtca tgggaaaagt ctcatagtag gccttgtcct tttttacgtc taggacatta
    19861 tagataaagg taaccgtccc actttcacgg tggtaatcca tgtgggcgcg ctcgtttcta
    19921 tccagtgcgt attcaatggt ttccttgtcc aaaccgtaaa tttcagaaag gtcttctagt
    19981 ttattcaact ttcctaggtc taggtctatc caggtacaac cattgcccaa ctgcttttct
    20041 aaaaccatct tttctcctta ttaaactccc tctattgtac cacaaattac ttaaaataac
    20101 aggtctagtc tgtgcgtgag aaattgctaa caactgtgat atttcggtta gggacaaagt
    20161 gaagggcttg ctcctcgcta cgaagctgtg tcgtacgaat tcccacactg acaaccttac
    20221 ccgatacagt aatcggtcca tttgtcagaa cgacctcatc tcccacatcc agttgacgtt
    20281 caaagaggat gaaaaagcca ttgatgacat cagacagaaa gccttgggct cccataccaa
    20341 tcgctacccc agcaatacca gctccagcca gcaaactaga aactggcaaa cctaaaatcg
    20401 acaaaatgca gtagagtaaa aagaaatata gcgtataatt aaacacattt tctagtaaac
    20461 gtgagatggt tttttggcgt ccgacatcat gacgagacat ttttagagaa ggtttgacaa
    20521 ttctctgcac catggtatga agcatttttt tagcaatata aaatacaatc aaaagcagta
    20581 aaagagaaat gaccttggtt agaatattct cgataatggt tgtcacatct agcttttcaa
    20641 tataagcctg aataaatttt tgcatataaa actcctttat ttcattatac catattttca
    20701 tcagtctcat tctccataaa tattcaaaaa atagacagaa aattcttgat tttgtctatc
    20761 atttatacta taatcataat cactacacaa aaaggagatt attatgaaaa gaatcattca
    20821 tgcttggaat aaggcaagcc ttgtcaagcg tatcttgatt ggtatgcttt gtggggaatc
    20881 ctaggactga cccttcctaa tctctcaggg attgggctac tcggggattt attcgttgga
    20941 ggtctaaaag ctgttgctcc tatcctagtc tttgctctcg ttgccaatgc cctttcccaa
    21001 catcaaaagg gacaagatag caatatgaaa actgttgttt tcttgtatat cctatgatag
    21061 ggactttcgc cgctgctctt gtagctgtac tagcaagttt catcgtccct attgaaatta
    21121 ccctaaatag tgccaatact gaaattgcac caccagatgg gattgggcag gtcctcagca
    21181 acctcttgct caaactggtt gacagcccag tcaacgccct gcttactgct aactatattg
    21241 gaatcttatc ttgggcagtc atttttggaa tcgctatgag agaagccagt aaaaatagta
    21301 aagaattgct aaaaactatc gctgacgtga cttctaaaat tgtcgaatgg atcatcaatc
    21361 tggctccatt tggaatcctt ggtcttgttt ttaaaaccat ttctgacaag ggagtcggaa
    21421 gccttgccaa ctacggtatt ttattggttc tattagtaac gactatgctt tttgttgccc
    21481 ttgtggtcac cctttgattg ccttcttctt tatgagacgc aatccttacc ctctagtttg
    21541 gaactgcctc cgtgtcagcg gtgtgacagc ctttttcact cgtagttctg cggctaacat
    21601 tcctgtcaac atgaaactct gccatgacct tggactcaac ccagatacct attctgtttc
    21661 tatcccactc ggttctacta tcaatatggc tggagtagcg attaccatta accttttgac
    21721 ccttgctgca gttaacactc ttggaattcc tgttgacttt gccacagcct ttgtcctcag
    21781 tgtggtagca gctatctcag cctgtggtgc ttcaggtatt gccggaggtt ccctccttct
    21841 tatcccagtt gcttgtagcc ttttcggtat ttctaacgat attgccatac aagttgtttg
    21901 ggttggtttt gtgattggtg tcatccaaga ctcatgtgaa acagccctta actcttctac
    21961 agaagatgtc ctctttaccg ccgttgccga atacgcagca acccgtaaaa aataactcat
    22021 caaggcaagc ctgcttatgt cttgtctttt acgcttttat tctaacttat taggaaattc
    22081 ttatgtctat tagccaacgt acgaccaagc tcatcttagc tacctgtctt gcctgcctgc
    22141 ttgcttattt tctcaatctt tcatcagcag tttcggctgg aattatcgct ctcttgagcc
    22201 tatctgatac gcgtagaagt actttaaaac tggctcgcaa tcgtcttttt tctatgcttc
    22261 tagctctggc tatcggtgtt ctagcttttc acttgagcgg atttcatatc tggagtctcg
    22321 gcctctatct ggccttctac gttcctttag cctacaagat gggctgggaa attggcatca
    22381 caccaagcac tgttttggtt agccatctct tggttcaaga gtcaacctct ccagaccttc
    22441 tagtcaatga attccttctc tttgctattg gtacaggatt tgccttgctt gttaatctct
    22501 atatgccttc acgagaagag gaaatccagc actaccacac gctggtggaa gaaaagttaa
    22561 aagatatcct ccagcgcttc aaatactatt tatccagagg agacggacgc aaccgagcac
    22621 agctggtagc agaattagac acgcttttga aagaagccct cagactggtc tatttggatc
    22681 actctgacca cctctttcac cagaccgact accatatcca ctactttgag atgagacaac
    22741 gacaaagtcg tatcctgaga aacatggccc aacagattaa cacttgtcac cttgccgcca
    22801 gtgaaagcct gatcttagcg caactctttt caaaaattgc aggtcaactg agccagacca
    22861 atcctgcttc tgatttgcta gatgaaattg aacgttatct ggaagtcttc cggaaccgca
    22921 gtctgcccaa gacaagagaa gagtttgaaa cccgcgccac tcttcttcaa ctcctacgtg
    22981 aagccaaaac cttcatccaa gtaaaagttg acttttatca aaaatataga cagtaatact
    23041 cttcaaacta cgtcagcttc accttgctgt atatatgtta ctgacttcgt cagttctatc
    23101 cacaacctca aaacagtgtt ttgagctgac ttcg
//
LOCUS       DAVKQL010000033        22782 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVKQL010000033 DAVKQL010000000
VERSION     DAVKQL010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22782
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.033"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            77..484
                     /locus_tag="VW561_001246"
     CDS             77..484
                     /locus_tag="VW561_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9943507.1"
                     /translation="MARYGVYAVIPNPEQKQIVLVQEPNGAWFLPCGEIEAGENHQEA
                     LKRELIEELGFTAEIGTYYGQADEYFYSRHRDTYYYNPAYLYEATSFKEVQKPLENFN
                     HIAWFPIDEAIQNLKRGSHKWAIESWKKQHKIG"
     gene            649..948
                     /locus_tag="VW561_001247"
     CDS             649..948
                     /locus_tag="VW561_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEU9943508.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKTSVDEANIKTLYSEKLIGISIPIK"
     gene            1209..1549
                     /gene="tnpB"
                     /locus_tag="VW561_001248"
                     /pseudo
     CDS             1209..1549
                     /gene="tnpB"
                     /locus_tag="VW561_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849103.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(1582..1890)
                     /locus_tag="VW561_001249"
                     /pseudo
     CDS             complement(1582..1890)
                     /locus_tag="VW561_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1881..2279
                     /locus_tag="VW561_001250"
     CDS             1881..2279
                     /locus_tag="VW561_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9943509.1"
                     /translation="MKSPIPKAPLAHNFGSASIIAHTIHQKFNLKVPNYRQEEDWARM
                     GLPITRKEISNRHIKTSQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHVLIDLFISYFNPL"
     gene            complement(2476..3396)
                     /gene="cysK"
                     /locus_tag="VW561_001251"
     CDS             complement(2476..3396)
                     /gene="cysK"
                     /locus_tag="VW561_001251"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEU9943510.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(3681..4316)
                     /locus_tag="VW561_001252"
     CDS             complement(3681..4316)
                     /locus_tag="VW561_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEU9943511.1"
                     /translation="MEFRTIKEDDQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            4372..5670
                     /locus_tag="VW561_001253"
     CDS             4372..5670
                     /locus_tag="VW561_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9943512.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPML
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            5667..6329
                     /locus_tag="VW561_001254"
     CDS             5667..6329
                     /locus_tag="VW561_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEU9943513.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQFWCKEGVGVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            6409..6957
                     /gene="raiA"
                     /locus_tag="VW561_001255"
     CDS             6409..6957
                     /gene="raiA"
                     /locus_tag="VW561_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEU9943514.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNVVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            7093..9066
                     /locus_tag="VW561_001256"
     CDS             7093..9066
                     /locus_tag="VW561_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEU9943515.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTSLLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            9063..9515
                     /gene="rplI"
                     /locus_tag="VW561_001257"
     CDS             9063..9515
                     /gene="rplI"
                     /locus_tag="VW561_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU9943516.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            9559..10911
                     /gene="dnaB"
                     /locus_tag="VW561_001258"
     CDS             9559..10911
                     /gene="dnaB"
                     /locus_tag="VW561_001258"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU9943517.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            10913..11179
                     /locus_tag="VW561_001259"
     CDS             10913..11179
                     /locus_tag="VW561_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943518.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            11268..12614
                     /gene="cbpD"
                     /locus_tag="VW561_001260"
     CDS             11268..12614
                     /gene="cbpD"
                     /locus_tag="VW561_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEU9943519.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEMPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRV
                     IKANTMTGFIHFKDLDGGSVGNSQSSASTGGTHYFKTKSAIKPEPLASGTVIDYYYPG
                     EKVHYDQIFEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSATVIDYYYPGENVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSHSSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGLWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      12697..12799
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            12870..13160
                     /locus_tag="VW561_001261"
     CDS             12870..13160
                     /locus_tag="VW561_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEU9943520.1"
                     /translation="MKASIALQVLPLVQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            13123..13881
                     /locus_tag="VW561_001262"
     CDS             13123..13881
                     /locus_tag="VW561_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9943521.1"
                     /translation="MRNLRSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     KPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRS"
     gene            13920..14927
                     /locus_tag="VW561_001263"
     CDS             13920..14927
                     /locus_tag="VW561_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9943522.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            14927..15655
                     /locus_tag="VW561_001264"
     CDS             14927..15655
                     /locus_tag="VW561_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9943523.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            15774..16232
                     /locus_tag="VW561_001265"
     CDS             15774..16232
                     /locus_tag="VW561_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEU9943524.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQIIQEVDRKGK"
     gene            16234..18666
                     /locus_tag="VW561_001266"
     CDS             16234..18666
                     /locus_tag="VW561_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9943525.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVFYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGNTLKI
                     GVKAGQLKFDIA"
     gene            18987..19640
                     /gene="rr06"
                     /locus_tag="VW561_001267"
     CDS             18987..19640
                     /gene="rr06"
                     /locus_tag="VW561_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEU9943526.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDLWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            19637..20977
                     /gene="hk06"
                     /locus_tag="VW561_001268"
     CDS             19637..20977
                     /gene="hk06"
                     /locus_tag="VW561_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEU9943527.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGEVGKQINGMYEHLLKV
                     IHELESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIA
                     KSINKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIEN
                     QLTDATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQE
                     LEHLFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            21060..21587
                     /locus_tag="VW561_001269"
     CDS             21060..21587
                     /locus_tag="VW561_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEU9943528.1"
                     /translation="MVLAIILVTFLIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDSMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYMTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            21666..>22782
                     /locus_tag="VW561_001270"
     CDS             21666..>22782
                     /locus_tag="VW561_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RICH domain-containing protein"
                     /protein_id="HEU9943529.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLFLGGVVHAEEVGGRN
                     TPTVTSSGQDISKKYADEVESHLKKILSEIQTQLDRKRHTETVALINELQGIKKTYLY
                     NLNVLKEKSKVELPSKIKAKLDVAFDQFKKDTLKPGEKVAEAQKKVAEAKKKAEDQKE
                     EDRRNYPTNTYKTLELDIAESDVKVKEAELELVNEEAKPRNEEKIKKAKAKVESEKAE
                     AIRLEEIKTDREEAKRKADAKLKEAVEKNAANSEQGEPKRRVKRGVLGEPATPDKKEN
                     DAKSSDSSVGEETLPSPSLKPEKKVAEAEKKVEEAKKKAEDQKEEDRRNYPTNTYKTL
                     ELDIAESDVKVKEAELELVNEEAKPRNEEKIKKAKAKV"
BASE COUNT         7156 a         3818 c         5100 g         6708 t
ORIGIN      
        1 taataacgat actagataat gaggaaaatg caatggcaaa cccaactttc ggagaaaaaa
       61 agcgaataca gactatattg ctcgctatgg cgtatatgca gttatcccta atcctgaaca
      121 aaaacaaatt gttcttgttc aagaaccaaa tggtgcttgg ttcctaccat gtggagaaat
      181 tgaagcaggt gaaaatcatc aggaagccct aaagcgtgag ttgattgaag agcttggttt
      241 cacagcagaa attggtacct attacggaca agctgacgaa tatttctatt ctcgtcatcg
      301 tgatacctac tactacaatc ctgcctacct ctatgaagca acttctttca aagaagtaca
      361 aaagccacta gaaaacttta atcatattgc ctggttccct attgacgagg ctatccaaaa
      421 ccttaaacgt ggtagccata aatgggccat tgaatcttgg aaaaaacagc ataagattgg
      481 ctaatactct tcgaaaataa aattcaaacc acgtcagcgt cgccttaccg tactcaagta
      541 tagcctgcgg ctagcttcct agtttgctct ttgattttca ttgagtataa atcaagcgac
      601 tttattcaaa aagtgctata atagaattag aaaatcggag gaaatattat gaagcttatc
      661 aatacgacaa actcacactc gcaactggta aaaagtcagc tagaaagtac cgatgcaacc
      721 cttgttgagg tttattctgc agggaataca gatgttattt ttacccaagc tccgcttcac
      781 tatgaaatcc tcatctcaaa caaacaccgt gctattcgtg agcctgaaat cgaaaccatt
      841 caagaatttt tcttgaaacg taaaattgat aagacctctg ttgatgaagc caatatcaag
      901 acactctact cagagaaatt aattggaatt tcaatcccaa tcaagtaaga aaattgagta
      961 aaaactcgat ttgagagaaa gtccaataag tctttccata acaaaacgca tagtaacaag
     1021 agtttctaca cccactatta tgcgttttta tcattttgaa atctactgct aatcaatgaa
     1081 gaagcaaaga tcaaactagg cagctagtta caagttacgt aagcgcgtca taacaaggta
     1141 tctatcctaa ttccccaact tccagtatac tagataaaaa ataattatcg gaggaaaggt
     1201 attgtactat gataattcaa ctaagtgatt taggtcaagt tcaccttgtt tgtagcaaga
     1261 tagatatgag gcagggaata gactcattag cctatgtagt taaaacccac tttgaattgg
     1321 atcctttctc cggtcaagtc tttctctttt gtggtggacg taaagaccgc tttaaagccc
     1381 tttactggga tggtcaagga ttttggctac tatataaacg ctttgagaac gtcagactga
     1441 cttggcccag tacagaaaag gatgtcaaag ctctcacacc tgaacaagta gactggctta
     1501 tgaagggctt ttctatcact ccaaaaataa atccatcaga aagtcgtgat ttctattgaa
     1561 atgaggactt tctttttata ctcatctgct ttcaaaaagc attctagtcc atctccgatt
     1621 aacgatggac tttatcacct ccttctccag tccttgtata acatcttgga gttgattcat
     1681 gacatcttcc aaagtttaaa aggctttatt cttaaatcca cgtttacgaa tctctttcca
     1741 cacttgttca atggggttca tctctggtgt gtatggagga ataaatgcaa agccaatatt
     1801 agtcggaatc tttaaggtac ttgatttatg ccatatagca ttgtccataa cgagtaaaag
     1861 ataatcatct ggataagctt gtgaaatctc ctattcctaa agccccttta gcgcataact
     1921 ttggctcagc ttctattatc gctcacacca tccatcagaa gtttaatctg aaggtaccca
     1981 attatcgcca agaagaagat tgggctagga tgggtttacc aatcacacgt aaggaaatct
     2041 ctaatcggca tatcaagacg agtcaatact atttggagcc cctttataac ctcttgcgag
     2101 agagactatt gactcagccc ttacttcatg cggatgaaac ttcttatagg gtgctagaga
     2161 gtgatagtca gctgacttac tattggactt ttttgtctgg gaaagctgag aaacaaggga
     2221 ttacgcttta ccatcatgtc ctaatcgatt tgttcatatc ttatttcaat ccattataaa
     2281 tagtgagaaa tatctatcct atcttctaga atgtcttcca aacgtggaaa ctctcgtaaa
     2341 caaagaggtt ttagaggcct atttaccatg gactaaagtt gtacaagaaa agtgcaaata
     2401 agaaatctcc agattaggaa ctatccgtga gttctctagt ctggagattt ttcaatagac
     2461 ttcgttattg gacggttaca attcataaag tgctgtagag agataacgtt caccgttatc
     2521 tggtgctagg gcaaggactt ttttacctgt acctaatttt ttggcaacct cgatggctcc
     2581 gtagatagct gcagctgagg aaatccctac aaggaagcct tcttttccac caatttcacg
     2641 tccgagtgca agagcgtcat ctgatgttac acgaacgata ccatcatagg ctttagtatc
     2701 aagtgtatca ggaataaatc cagctgagat accttgaatt ttgtgaggac caggtttttc
     2761 accagataga atagcagatt catctgcttc tactgcaaaa acttgaatgt tagaattttc
     2821 tgatttgagt gcatgagaaa caccagaaat cgttccaccg gtacctactc cagcaacaaa
     2881 ggcatctaat ccatctttac cgaaagcagc tagtatctca gctcctgttg ttctttcgtg
     2941 tacttctgga ttagctggat tgtcaaattg aagaggaagg aaaccatcac gttcagcagc
     3001 gatttcttga gccttagcaa tagcaccttt cattccctcg ctaccaggag ttaggacgag
     3061 ttcagcacca taagcttgga taattttacg tcgttctaca ctcatagttt caggcataac
     3121 gatgacgact ttataccctt tagcagcacc tacccatgaa agtccaatac cagtgtttcc
     3181 acttgttgct tcaacaatag tagaaccagg tttcagaata ccatcttgtt cagctttttc
     3241 aatcatgcta agggcaatac ggtcttttac agatgaacca ggattaaatg cttcaagctt
     3301 tatatagacg tctgcagcac cttctggcac gatgttgtta agtttaacaa tcggtgtttg
     3361 accgattaat tcagtaatgt tgttataaat agacatatga atacctcttt gtataagata
     3421 tctttagtat aacgctatcc ataccatttg taaaatatag aaatcctatc gaagcgatag
     3481 gatttctata tatcatagat caaagtcatt aattttcaag tgattgcgtt cagctaattc
     3541 tagtaaatag gtgtgaagga aaacgatatc aggtttcgtt tgaattgctt cattttctag
     3601 tatattttgg tacgcatctt tttattttgc tcgctaaagt agttgtgaga atgtcaacga
     3661 aacgctgtat tattcattgt ttacactaag tttacaggaa cttcaacctc tcgtaaacct
     3721 tggtcagtta aagtgacttt tccattaaaa aactccacaa gtgcagcttt aatagtttct
     3781 ttttcttctt tatcaacata aatcatcgta tcgacttgat ctgtaaagtt tgtatccagc
     3841 tccatgagat catgttcttt aaggaagtta ctgtactctt ggtactgagc ataagacatt
     3901 tgaatagcaa tgccagcctg ttcttttatt tcaataatac caatttcttt gacagctaag
     3961 gcgacactgc cggcgtaagc acgaattagt cctccagcgc ctagtttaat accaccaaag
     4021 tagcgtgtca cgaccacaca gacattggtg agattgtgat tttctagtac cccaagcatg
     4081 ggaacaccag cagtaccact aggctcacca tcatcacttg tacgtttaat ttcactacgt
     4141 tctccaataa taaaggcaga gcagttatgt gtcgctttgt agtgttcttt tttgatggca
     4201 gtaatgaagt cacgagcctc ttcttcgcta taaacacgct tggcatggca gataaagcga
     4261 gattttttga tttcttcttg gacttggtcg tcctctttaa ttgttctaaa ttccatgatt
     4321 taattgtact aaaaaatcat ctaaagtgct agtttttacg aataaagaag tatgaaagta
     4381 aatttagatt atctcggtcg tttatttact gagaatgaat taacagaaga agaacgtcag
     4441 ttggcggaga aacttccagc aatgagaaag gagaagggga aacttttctg tcaacgttgt
     4501 aatagtacta ttctagaaga atggtatttg cccatcggtg cttactattg tcgagagtgc
     4561 ttgctgatga agcgagtcag aagtgatcaa actttatact attttccgca ggaggatttt
     4621 ccgaagcaag atgttctcaa atggcgcggc caattaactc cttttcaaga gaaggtgtca
     4681 gagggactgc ttcaagcagt agacaagcaa aagccaacct tagttcatgc ggtaacagga
     4741 gctggaaaga cagaaatgat ttatcaagta gtggctaaag tgatcaatgc gggtggtgca
     4801 gtgtgtttgg ctagtcctcg catagatgtt tgtttggagc tgtacaagcg cctgcaacag
     4861 gatttttctt gcgggatagc tttgctacat ggagaatcgg aaccttattt tcgaacacca
     4921 ctagttgttg caacaaccca tcagttattg aagttttatc aagcttttga tttgctgata
     4981 gtggatgaag tagatgcttt tccttatgtt gataatccaa tgctttacca cgctgtcaag
     5041 aatagtgtaa aggagaatgg attgagaatc tttttaacag cgacttcgac caatgagtta
     5101 gataaaaagg tccgtttagg agaactaaaa agactgaatt taccgagacg gtttcatgga
     5161 aatccgttga ttattccaaa accaatttgg ttatcggatt ttaatcgcta cttagacaag
     5221 aatcgtttgt caccaaagtt aaagtcctat attgagaagc agagaaagac agcttatccg
     5281 ttactcattt ttgcttcaga aattaagaaa ggggagcagt tagaagaaat cttacaggag
     5341 caatttccaa atgagaaaat tggctttgta tcttctgtaa cagaggatcg attagagcaa
     5401 gtacaagctt ttcgagatgg agaactgaca atacttatca gtacgacaat cttggagcgt
     5461 ggagttacct tcccttgtgt ggatgttttc gtagtagagg ccaatcatcg tttgtttacc
     5521 aagtctagtt tgattcagat tggtggacga gttggacgaa gcatggatag accgacagga
     5581 gatttgcttt tcttccatga tgggttaaat gcttcaatca agaaggcgat taaggaaatt
     5641 cagatgatga ataaggaggc tggtctatga agtgcttgtt atgtgggcag actatgaaga
     5701 ctgttttaac ttttagtagt ctcttacttc tgaggaatga tgactcttgt ctttgttcag
     5761 actgtgattc tacttttgaa agaattgggg aagagaactg tccaaattgt atgaaaacag
     5821 agttgtcaac aaagtgtcaa gattgtcaat tttggtgtaa agaaggagtt ggagtcagtc
     5881 atagagcgat ttttacttac aatcaagcta tgaaggattt tttcagtcgg tataagtttg
     5941 atggagactt cctgttaaga aaagttttcg cttcattttt aagtgaggag ttgaaaaagt
     6001 acaaagagta tcaatttgtt gtaattcccc taagtcctga tagatatgct aatagaggat
     6061 ttaatcaggt tgagggcttg gtagaggcag caggttttga gtatctggat ttattagaga
     6121 aaagagaaga gagagccagt tcttctaaaa atcgttcaga gcgcttgggg acagaacttc
     6181 ctttctttat taaaagtgga gtcactattc ctaaaaaaat cctacttata gatgatatct
     6241 atactacagg agcaactata aatcgtgtta agaaactgtt ggaagaagct ggtgctaagg
     6301 atgtaaaaac attttccctt gtaagatgag gaaaaaattt gcaaaaatag aatgaaagcg
     6361 ttataataaa atcataaaaa caaaaatgtt tagaaagaag gtactcatat gattaaatat
     6421 agtatccgtg gtgaaaacct agaagtaaca gaagcaattc gtgattatgt agtttctaaa
     6481 ctcgaaaaga tcgaaaagta cttccaacca gaacaagagt tggatgcccg aattaactta
     6541 aaagtttatc gtgaaaaaac ggctaaagtg gaagtaacga ttccgcttgg atctattact
     6601 ctccgcgcag aagatgtatc tcaagatatg tatggttcaa ttgaccttgt aactgataaa
     6661 attgaacgtc agattcgtaa aaataaaaca aaaatcgagc gtaaaaataa aaataaggta
     6721 gcaactggtc aattatttac agatgctttg gtggaagatt caaatgttgt ccagtctaaa
     6781 gttgttcgtt caaaacaaat tgatttaaaa ccaatggatt tggaagaagc aattctacaa
     6841 atggatttat tggggcatga tttctttatc tatgtggatg ttgaagatca gacaaccaat
     6901 gtgatttatc gtcgtgagga tggcgaaatt ggtttgttag aggttaaaga atcttaattt
     6961 caatatgtgt aaaggtaggt ttactgaatt gtaaactacc tttttcttat aattgataga
     7021 attactgatt acacttttaa aaagtcgctt tttgaggggt attatggtat aatgggtgcc
     7081 aagaggtttt gaatgaaaaa attttatgta agtccaattt ttcctattct agtaggattg
     7141 attgcgtttg gagtcttatc cactttcatt atttttgtta ataataatct gttgacggtt
     7201 ttaattttgt ttctttttgt aggaggctat gtttttttat ttaagaaact gagagtgcat
     7261 tatacaagga gtgatgtaga acagatacag tatgtaaacc accaagcgga agaaagtttg
     7321 acatctctat tggaacagat gcctgtaggt gttatgaaat tgaatttatc ttctggagag
     7381 gttgagtggt ttaatcccta tgctgaattg attttgacca aggaagatgg tgattttgat
     7441 ttagaagctg ttcaaacgat tattaaggct tcagtaggaa atccgtctac ttatgccaag
     7501 cttggtgaga agcgttatgc tgttcatatg gatgcttctt ccggtgtttt gtattttgta
     7561 gatgtatcca gggaacaagc cataacagat gaattggtaa caagtcgacc agtgattggg
     7621 attgtctctg tggataatta tgatgatttg gaggatgcaa cttctgagtc agatattagt
     7681 caaatcaata gttttgtagc taattttata tcagagtttt cagaaaaata catgatgttt
     7741 tctcgtcggg taagtatgga tcgattttat ctatttactg actacacggt gcttgagggc
     7801 ttgatgaatg ataaattttc tgttattgat gctttcagag aagagtcgaa acagagacag
     7861 ttgcccttga ccttaagtat gggattttct tatggcgatg gaaatcatga tgagataggg
     7921 aaagttgctt tgctcaattt gaacttggct gaagtacgtg gtggcgacca ggtggttgtt
     7981 aaggaaaacg acgaaacgaa aaatccagtt tattttggtg gtgggtctgc tgcttcaatc
     8041 aagcgtacac ggactcgtac gcgcgctatg atgacagcta tttcagataa gattcggagt
     8101 gtagatcagg tttttgtagt cggtcacaaa aatttagaca tggatgcttt gggctctgct
     8161 gtaggtatgc agttgttcgc cagcaatgtg attgaaaata gctatgctct ttatgatgaa
     8221 gaacaaatgt ctccagatat tgaacgagct gtttcattca tagaaaaaga aggagttacg
     8281 aagttgttgt ctgttaagga tgcaatgggg atggtgacca atcgttcttt gttgattctt
     8341 gtagaccatt caaagacagc cttaacatta tcaaaagaat tttatgattt atttacccaa
     8401 accattgtta ttgaccacca tagaagggat caggattttc cagataatgc ggtcattact
     8461 tatatcgaaa gtggtgcaag tagtgccagt gagttggtaa cggaattgat tcagttccag
     8521 aattctaaga aaaatcgttt gagtcgtatg caagcaagtg ttttgatggc tggtatgatg
     8581 ttggatacta aaaatttcac ctcgcgagta actagtcgga catttgatgt tgctagctat
     8641 ctcagaacgc gcggaagtga tagtattgct atccaggaaa tcgctgcgac agattttgaa
     8701 gaatatcgtg aggtcaatga acttatttta caggggcgta aattaggttc agatgtacta
     8761 atagcagagg ctaaggacac gaaatgctat gatacagttg ttattagtaa ggcagcagat
     8821 gccatgttag ccatgtcagg tattgaagcg agttttgttc ttgcgaagaa tacacaagga
     8881 tttatctcta tctcagctcg aagtcgtagt aaactgaatg tacaacggat tatggaagag
     8941 ttaggcggtg gaggccactt taatttggca gcagctcaaa ttaaagatgt aaccttgtca
     9001 gaagcaggtg aaaaactgac agaaattgta ttaaatgaaa taaaggaaaa ggagaaagaa
     9061 gaatgaaagt aatcttttta gcagatgtta aaggaaaagg taaaaaaggc gaaattaagg
     9121 aagtaccaac agggtatgcg caaaactttc ttatcaaaaa gaatctagcc aaagaagcga
     9181 ctgctcaagc tgtaggtgaa cttcgtggta aacaaaaatc ggaagaaaaa gctcacgctg
     9241 agatgattgc agaaggaaaa gcaattaaag cacaacttga agcagaagaa actgttgtag
     9301 aatttgttga aaaagttggt ccagatggtc gtacctttgg ttctattacc aataagaaga
     9361 ttgcagaaga attgcaaaag caatttggaa ttaagattga taaacgtcat attcaagtac
     9421 aagctccgat tcgagcggtt ggtttgattg atgtgccagt gaaaatctat caagatatca
     9481 caagtgtaat caatcttcgt gtgaaagaag gataagttta caccttcttg acaagattgt
     9541 aaaaggaagg gaagtctgat ggcagaagta gaagagttac gagtacaacc tcaagatatc
     9601 ttagctgagc aatccgtttt aggggctatc tttattgatg agagtaaact tgtttttgtg
     9661 cgagaataca ttgagtctcg ggactttttt aagtatgccc atcgtttgat tttccaagcc
     9721 atggtcgatt tatccgatcg tggtgatgcc atagatgcaa caacggttcg tactatcctt
     9781 gataatcaag gtgatttaca gaatattggt ggcttgtctt acttggttga gattgtcaat
     9841 tctgtgccaa cttctgctaa tgcggagtat tatgctaaga ttgttgcaga aaaagcaatg
     9901 ctacgtcgtt taattgccaa gttgacagag tctgtcaacc aagcttacga agcgtcacaa
     9961 ccagctgatg aaattattgc tcaggcagaa aaagggctga ttgatgtcag tgaaaatgca
    10021 aatcgaagcg ggtttaagaa cattcgagat gtgttgaatc tcaactttgg aaatctggaa
    10081 gctcgctcgc aacaaacgac cgatattaca ggtattgcga caggttatcg tgatttggat
    10141 catatgacaa caggacttca tgaggaggag ttgattatct tagcagctcg tccagcagtt
    10201 ggtaagacag catttgcctt gaatatcgct cagaacattg ggactaagtt ggacaaaacg
    10261 gttgctattt tttcactcga aatgggtgcg gaaagcttgg tagatcgtat gttagctgca
    10321 gaaggcttag tggagtcaca ttctatccgt acagggcaat tgacagatga ggagtggcaa
    10381 aaatatacta ttgctcaggg taatctagct aacgccagta tctatatcga tgatacgcca
    10441 ggtattcgga ttacagagat tcgttctcgt tctcgtaaat tggctcaaga aactggaaat
    10501 cttggtttga ttttgataga ctatttgcaa cttatcacgg gaactggtcg agaaaatcgt
    10561 caacaagaag tttctgaaat ttctcgtcag ttgaaaatac tagccaagga attgaaggtt
    10621 ccagtaatcg ctctgagtca gctttctcgt ggtgtagaac aacgtcagga caagagaccg
    10681 gtcttgtctg atattcgtga atctgggtct attgagcagg acgctgatat cgtagctttt
    10741 ctctatcgcg atgactacta tgaacgtggt ggtgaagaag aggagggtat cccaaataat
    10801 aaggtggaag ttattatcga gaaaaaccgt agtggagctc gtggaacagt ggaattgatt
    10861 ttccaaaaag aatacaataa attttcaagt atctcaaaga gggaggcata agatgtcaga
    10921 tgcatttaca gatgtagcca agatgaaaaa aatcaaagaa gaaatcaagg cacatgaggg
    10981 acaagtcgta gaaatgactt tggagaatgg tcgtaagcgc caaaaaaata gattgggtaa
    11041 gctaattgaa gtttatccat ccctatttat tgtggagttt ggggatgtgg aaggagataa
    11101 acaagttaat gtttacgttg aatcctttac ttactcagat attcttacag aaaagaattt
    11161 gattcattat cttgactaaa gtgagaaatt ttctcacttt ttcttttttc tccgaataat
    11221 ttaggtgaag gcaatcatcg ctttatatta tttttcaagg aggaagaatg aaaattttac
    11281 cgtttatagc aagaggaaca agttattact tgaagatgtc agttaaaaag cttgttcctt
    11341 ttttagtagt aggattgatg ctagcagctg gtgatagtgt ctatgcctat tccagaggaa
    11401 atggatcgat tgcgcgtggg gatgattatc ctgcttatta taaaaatggg agccaggaga
    11461 ttgatcagtg gcgcatgtat tctcgtcagt gtacttcttt tgtagccttt cgtttgagta
    11521 atgtcaatgg ttttgaaatg ccggcagctt atggaaatgc gaatgaatgg ggacatcgtg
    11581 ctcgtcggga aggttatcgt gtagataata caccgacgat tggttccatt acttggtcta
    11641 ctgcaggaac ttatggtcat gttgcctggg tgtcaaatgt aatgggagat cagattgaga
    11701 ttgaggaata taactatggt tatacagaat cctataataa acgagttata aaagcaaaca
    11761 cgatgacagg atttattcat tttaaagatt tggatggcgg cagcgttggg aatagtcaat
    11821 cctcagcttc aacaggcgga actcattatt ttaagaccaa gtctgctatt aaacctgaac
    11881 ccctagctag cggaactgtg attgattact attatcctgg ggagaaggtt cattatgatc
    11941 agatatttga aaaagacggc tataagtggt tgagttatac tgcctataat ggaagctatc
    12001 gttatgttca attggaggct gtgaataaaa atcctctagg taattctgtt ctttcttcaa
    12061 caggcggaac tcattatttt aagaccaagt ctgctatcaa aactgaaccc ctagttagtg
    12121 caactgtgat tgattactat tatcctggag agaacgttca ttatgatcaa attctcgaaa
    12181 aagacggcta caagtggttg agttatacgg cttataacgg aagtcgtcgc tatatacagc
    12241 tagagggagt gacttcttca caaaattatc agaatcaatc aggaaacatc tctagctatg
    12301 gatcccatag tagttcaact gtcggttgga agaaaataaa tggtagttgg tatcatttca
    12361 aatcaaatgg ttctaaatca acaggatggc tgaaagacgg ttctagctgg tattatttga
    12421 aattatctgg tgaaatgcag acaggatggt taaaggaaaa tggtttgtgg tattatctgg
    12481 gtagttcagg ggcaatgaaa acaggctggt accaggtctc tggtaagtgg tattattctt
    12541 actcttcagg cgccttagct gttaatacga cggtggatgg ctacagagta aacagtgatg
    12601 gagaacgagt atagaaaatt ggagtaggag aaatttcctg ctcttttctg taagcagttt
    12661 ccttgacatt tttcttattt tgcgctatca tatatacata taatatatgg gagtctgtgt
    12721 acagtctgag aggaagtgtt aaacttcgac cgcacctgat ctgggtaatg ccagcggagg
    12781 gaacgatact tagtctaatt ttgcaccttt tccatgtatg gtaaaggttt ttcttttttt
    12841 aaaaaggaaa acgagaagag gaggttctta tgaaagcaag cattgccttg caagttttac
    12901 ccctagtaca ggggattgat cggatagctg ttattgatca ggtcattgct tatctgcaaa
    12961 ctcaagaagt gacgatggta gtgacaccat ttgaaacggt cttggaaggg gagtttgatg
    13021 agcttatgcg cattctaaaa gaagcgctgg aagtggcagg gcaggaggca gacaatgtct
    13081 ttgccaatgt caaaataaat gtaggagaga ttttaagtat tgatgagaaa cttgagaagt
    13141 atactgagac gacacattag tctattgggc tttctcggag tattgtcaat ctggcagtta
    13201 gcaggttttc ttaaacttct ccccaagttt atcctgccga cacctcttga aattctccag
    13261 ccctttgttc gtgacagaga atttctctgg caccatagct gggcgacctt gagagtggct
    13321 ttactggggc tgattttggg agttttgatt gcctgtctta tggctgtgct catggatagt
    13381 ttgacttggc tcaatgacct gatttaccct atgatggtgg tcattcagac cattccgacc
    13441 attgccatag ctcctatcct ggtcttgtgg ctaggttatg ggattttgcc caagattgtc
    13501 ttgattatct taacgacaac ctttcccatc atcgttagta ttttggacgg ttttaggcat
    13561 tgcgacaagg atatgctgac cttgtttagt ctgatgcggg ccaagccttg gcaaatcctg
    13621 tggcatttta aaatcccagt tagcctgcct tacttttatg caggtctgag ggtcagtgtc
    13681 tcctacgcct ttatcacaac tgtggtatct gagtggttgg gaggttttga aggtcttggt
    13741 gtttatatga ttcagtctaa aaaactgttt cagtatgata ccatgtttgc cattattatt
    13801 ctggtgtcga ttatcagtct tttgggtatg aagctggtcg atatcagtga aaaatatgtg
    13861 attaaatgga aacgttcgta gaattagaat gtttctgaaa aagaaaagag gaaatcaaaa
    13921 tgaagaaaac atggaaagtg tttttaacgc ttgtaacagc tcttgtagct gttgtgcttg
    13981 tggcctgtgg tcaaggaact gcttctaaag acaacaaaga ggcagaactt aagaaggttg
    14041 actttatcct agactggaca ccaaatacca accacacagg gctttatgtt gccaaggaaa
    14101 aaggttattt caaagaagct ggagtggatg ttgatttgaa attgccacca gaagaaagtt
    14161 cttctgactt ggttatcaac ggaaaggcac catttgcagt gtatttccaa gactacatgg
    14221 ctaagaaatt ggaaaaagga gcaggaatca ctgccgttgc agctattgtt gaacacaata
    14281 catcaggaat catctctcgt aaatctgata atgtaagcag tccaaaagac ttggttggta
    14341 agaaatatgg gacatggaat gacccaactg aacttgctat gttgaaaacc ttggtagaat
    14401 ctcaaggtgg agactttgag aaggttgaaa aagtaccaaa taacgactca aactcaatca
    14461 caccgattgc caatggcgtc tttgatactg cttggattta ctacggttgg gatggtatcc
    14521 ttgctaaatc tcaaggtgta gatgctaact tcatgtactt gaaagactat gtcaaggagt
    14581 ttgactacta ttcaccagtt atcatcgcaa acaacgacta tctgaaagat aacaaagaag
    14641 aagctcgcaa agtcatccaa gccatcaaaa aaggctacca atatgccatg gaacatccag
    14701 aagaagctgc agatattctc atcaagaatg cacctgaact caaggaaaaa cgtgactttg
    14761 tcatcgaatc tcaaaaatac ttgtcaaaag aatacgcaag cgacaaggaa aaatggggtc
    14821 aatttgacgc agctcgctgg aatgctttct acaaatggga taaagaaaat ggtatcctta
    14881 aagaagactt gacagacaaa ggcttcacca acgaatttgt gaaataatga cagaaattag
    14941 actagagcac gtcagttatg cctatggtca ggagaggatt ttagaggata tcaacctaca
    15001 ggtgacttca ggcgaagtgg tttccatcct aggcccaagt ggtgttggaa agaccaccct
    15061 ctttaatcta atcgctggga ttttagaagt tcagtcaggg agaattgtcc ttgatggtga
    15121 agaaaatccc aaggggcgcg tgagttatat gttgcaaaag gatctgctct tggagcacaa
    15181 gacggtgctt ggaaatatca ttctgcccct cttgattcaa aaggtggata aggcagaagc
    15241 tatttcccga gcggataaaa ttcttgcgac cttccagctg acagctgtaa gagacaagta
    15301 tcctcatgaa cttagcggtg ggatgcgcca gcgtgtagcc ttactccgga cctacctttt
    15361 tgggcacaag ctctttctct tagatgaggc ctttagcgcc ttggatgaga tgacaaagat
    15421 ggaactccac gcttggtatc ttgagattca caagcagttg cagctaacaa ccctgatcat
    15481 cacgcatagt attgaggagg ccctcaatct cagcgaccgc atctatatct tgaaaaatcg
    15541 ccctgggcag attgtttcag aaattaaact agattggtct gaagatgagg acaaggaagt
    15601 ccaaaagatt gcctacaaac gtcaaatttt ggcggaatta ggcttagata agtagaaaaa
    15661 tagggagttg gtgaagatta tcctttacca gcgccctttt tcttttaaaa atgagaaaat
    15721 ttcggtataa tagtcaaaca aggtcaaggt ttaaagagag aggtgggttt gttatgagat
    15781 ttaaaaatac atcggatcat attgaggcct acatcaaggc gattttagat caatctggta
    15841 tcgtggagtt gcaacggagt cagttggcag atacctttca ggttgttcct agtcagatta
    15901 actacgtgat caagacacgc tttacggaaa gtagaggcta cttggttgaa agtaagcgtg
    15961 gtggcggagg ctacattcgt ataggacgga ttgagttttc tagtcatcat gaaatgctcc
    16021 gggagctgct ttactcgatt ggtgagcgag tcagtcaaga aatttatgag gatattctcc
    16081 agcttttggt tgagcaggaa ttgatgacca agcaggagat gaatttgcta gaatcagtag
    16141 ctttggatcg cgttttagga gaagaagctc cagttgttcg agcaaacatg ctacgtcaga
    16201 tcatacaaga ggtagataga aaagggaagt aagatgaact attcaaaagc attgaatgaa
    16261 tgtatcgaaa gtgcctacat ggttgctgga cattttggag ctcgttatct agagtcttgg
    16321 cacttgttga ttgccatgtc taatcacagt tatagtgtag caggggcaac tttaaatgat
    16381 tatccgtatg agatggaccg tttagaagag gtggctttgg aactgactga aacggactat
    16441 agccaggatg aaacctttac ggaattgccg ttctcccgtc gtttgcaggt tctttttgac
    16501 gaagcagagt atgtagcgtc agtggtccat gctaaggtgc tagggacaga gcacgtcttc
    16561 tatgcgattt tgcatgatgg caatgccttg gcaactcgta tcttggagag ggctggtttt
    16621 tcttatgaag acaagaaaga tcaggtcaag attgctgccc ttcgtcgaaa tttagaagaa
    16681 cgggcaggct ggactcgtga agacctcaag gctttacgcc aacgccatcg tacagtagct
    16741 gacaagcaaa attctatggc caatatgatg ggcatgccgc agactcctag tggtggtctt
    16801 gaggactata cgcatgattt gacagagcaa gcgcgttctg gcaagttaga accagtcatc
    16861 ggtcgggaca aggaaatctc acgtatgatt caaattttga gccgtaagac taagaacaac
    16921 cctgtcttgg ttggggatgc tggtgtcggg aaaacagctc tggcccttgg tcttgcccag
    16981 cgtattgcta gtggtgacgt gcctgcggaa atggctaaga tgcgcgtgtt agaacttgat
    17041 ttgatgaatg tcgttgcagg gacacgcttc cgtggtgact ttgaagaacg catgaataat
    17101 atcatcaagg atattgaaga agatggccaa gtcatcctct ttatcgatga actccacacc
    17161 atcatgggtt ctggtagcgg gattgattcg actctggatg cggccaatat cttgaaacca
    17221 gccttggcgc gtggaacttt gagaacggtt ggtgccacca ctcaggaaga atatcaaaaa
    17281 catatcgaaa aagatgcggc tctttctcgt cgtttcgcta aagtgacgat tgaagaacca
    17341 agtgtggcag atagtatgac tattttacaa ggtttgaagg cgacttatga gaaacatcac
    17401 cgtgtacaaa tcacagatga agcggttgaa acagcggtta agatggctca tcgttattta
    17461 accagtcgtc acttgccaga ctctgctatc gacctcttgg acgaagcagc agcaacagtg
    17521 caaaataagg caaagcatgt aaaagcagac gattcagatt tgagtccagc tgacaaggcc
    17581 ctgatggatg gcaagtggaa acaggcagcc cagctaatcg caaaagaaga ggaagtacct
    17641 gtctacaaag acttggtgac agagtctgat attttgacca ccttgagtcg cttgtcagga
    17701 atcccagttc aaaaactgac tcaaacggat gctaagaagt atttaaatct tgaagcagaa
    17761 ctccataaac gtgtcatcgg tcaagatcaa gctgtttcaa gtattagtcg tgccattcgc
    17821 cgtaatcagt caggaattcg cagtcacaag cgtccgatcg gttcctttat gttcctagga
    17881 cccacaggtg tcggtaagac cgagttggcc aaggctttgg cagaagttct ctttgatgac
    17941 gaatcagccc ttatccgctt tgatatgagt gagtatatgg agaaattcgc agccagccgt
    18001 ctaaatggag ctcctccagg ctatgtgggt tacgaagaag gtggggagct gacagagaag
    18061 gttcgcaata aaccctattc cgttctcctc tttgatgagg tagagaaggc ccacccagat
    18121 atctttaatg ttctcttgca ggttctggat gacggtgtct tgacagatag caagggacgc
    18181 aaggtcgatt tttcaaatac cattatcatt atgacatcga atctaggtgc gactgccctt
    18241 cgtgatgata agactgttgg ttttggggct aaggatattc gttttgacca ggaaaatatg
    18301 gaaaaacgca tgtttgaaga actgaaaaaa gcttatagac cggaattcat caaccgcatt
    18361 gatgagaagg tggtcttcca tagcctatct agtgaccata tgcaggaagt ggtgaagatt
    18421 atggtcaagc ctttagtggc aagtttggct gaaaaaggca ttgacttgaa attacaagct
    18481 tcagctctga aattgttagc aaatcaagga tatgacccag agatgggagc tcgcccactt
    18541 cgcagaaccc tgcaaacaga agtggaggac aagttggcag aacttcttct taagggagat
    18601 ttagtggcag gcaacacact taagattggt gtcaaagcag gccagttaaa atttgatatt
    18661 gcataaaaga ataaaagtat cagcatctga ccataagtca cagtggagtg aaactcaatg
    18721 aaaatcaaag agcaaactag gcagctagct gcaggttgct caaaacactg ttttgaggtt
    18781 gcagatagag ctgacgtggt ttgaagagat tttcgaagag tataagctgc aagatgaatg
    18841 attttcttgt attgacgttg ttgttgacaa aaagtagcgg ataaatgaaa tccattccat
    18901 tatcatagat gataggctgg taggaaattt tcaaatagca tacaggaaat agatgtatgg
    18961 agttctggta gtagaaaggg agagagatga acattttagt tgcagatgac gaggaaatga
    19021 ttagagaagg aattgcagca tttctgacag aagagggtta tcatgtcatt atggctaagg
    19081 atggacaaga ggtcttggaa aaatttcaag atctccctat ccatctcatg gtactggatt
    19141 taatgatgcc taggaagagt ggttttgaag tgttaaaaga aatcaatcaa aagcacgata
    19201 ttcctgtcat cgtcttgagt gctctgggag atgaaactac tcagtcacag gtatttgatc
    19261 tctatgctga tgatcatgtg acaaaacctt tttctttggt actgcttgtc aagcgtatta
    19321 aggcgcttat cagacgttac tacgtcatag aggatctttg gcgatatcag gatgtaacag
    19381 tggattttac ctcttacaaa gcacattata aaaatgaaga aattgatctc aaaccaaagg
    19441 aattactggt actaaagtgt ttgattcagc ataaaaatca agttttaagt agagagcaga
    19501 tattggaaga aatttcaaaa gatgtagctg atttaccttg tgatagggtc gttgatgtct
    19561 atattcgtac tcttcgcaaa aaattagctt tagattgtat cgtgactgtg aaaaatgttg
    19621 ggtataagat tagcttatga taaaaaatcc taaattatta accaagtctt ttttaagaag
    19681 ttttgcaatt ctaggtggtg ttggtctagt cattcatata gctatttatt tgacctttcc
    19741 tttttattat attcaactgg agggggaaaa gtttaatgag agcgcaagag tgtttacgga
    19801 gtatttaaag actaagacat ctgatgaaat tccaagctta ctccagtctt attcaaagtc
    19861 cttgaccata tctgctcacc ttaaaagaga tattgtagat aagcggctcc ctcttgtgca
    19921 tgacttggat attaaagatg gaaagctatc aaattatatc gtgatgttag atatgtctgt
    19981 tagtacagca gatggtaaac aggtaaccgt gcaatttgtt cacggggtag atgtctacaa
    20041 agaagcaaag aatattttgc ttttgtatct cccatataca tttttggtta caattgcttt
    20101 ttcctttgtt ttttcttatt tttatactaa acgcttgctc aatcctcttt tttacatttc
    20161 agaagtgact agtaaaatgc aagatttgga tgacaatatt cgttttgatg aaagtaggaa
    20221 agatgaagtt ggtgaagttg gtgaagttgg aaaacagatt aatggtatgt atgagcactt
    20281 gttgaaggtt attcatgagt tggaaagtcg taatgagcaa attgtaaaat tgcaaaatca
    20341 aaaggtttcc tttgtccgcg gagcatcaca tgagttgaaa acccctttag ccagtcttag
    20401 aattatccta gagaatatgc agcataatat tggagattac aaagatcatc caaaatatat
    20461 tgcaaagagt ataaataaga ttgaccagat gagccactta ttggaagaag tactggagtc
    20521 ttctaaattc caagagtgga cagagtgtcg tgagaccttg actgttaagc cagttttagt
    20581 agatatttta tcacgttatc aagaattagc tcattcaata ggtgttacaa ttgaaaatca
    20641 attgacagat gctaccaggg tcgtcatgag tcttagggca ttggataagg ttttgacaaa
    20701 cctgattagt aatgcaatta aatattcaga taaaaatggg cgcgtaatca tatccgagca
    20761 agatggctat ctctctatca aaaatacatg tgcgcctcta agtgaccaag aactagaaca
    20821 tttatttgat atattctatc attctcaaat cgtgacagat aaggatgaaa gttccggttt
    20881 gggtctttac attgtgaata atattttaga aagctatcaa atggattata gttttctccc
    20941 ttatgaacac ggtatggaat ttaagattag cttgtagaca gattagtttt ttattaaagt
    21001 tcatataggg ttaacataag tgtgttattc tttgtgtaga taaaagaaag gatactaata
    21061 tggtattagc gattatttta gtaacattcc ttattcgatt gattttttta aagcgttcga
    21121 tagagaatga gaaacgaatc cttagcaatg gcgggaaaga atttggagtt gagaatacaa
    21181 aacgattaac tatggctcat attgtttttt atctctcttg cttggttgag gcaatggtgc
    21241 acaagacaat ttttgatagc atgggcatgg ttggtttagt cttgcttatt ttttctatgc
    21301 tgatgttaat gttggtgatt cacttgttgg gagatatttg gacagtgaag cttatgcttg
    21361 tcaataatca caaatatgta gatcatatct tgtttaggac agtaaaacat cctaattact
    21421 ttttaaatat tcttcctgag ttgattggct tgaccttgtt gagtcatgcc tatatgactt
    21481 ttgttttagt ttttccagtt tatgcagtta ttttgtatcg acgaatagct gaagaggaaa
    21541 agctattaca tgaagttata atcccaaatg gaagcataaa gagataaata caaaattcga
    21601 tttatataca gttcatattg aagtgatata gtaaggttaa agaaaaaata tagaaggaaa
    21661 taaacatgtt tgcatcaaaa agcgaaagaa aagtacatta ttcaattcgt aaatttagta
    21721 ttggagtagc tagtgtagta gttgctagct tgttcttagg aggagtagtt catgcagaag
    21781 aggttggtgg taggaatacc cccacggtta catctagtgg gcaagatata tcgaagaagt
    21841 atgctgatga agtcgagtcg catctaaaaa aaatattgag tgagatccaa acacaattag
    21901 atagaaaacg acataccgaa actgtagcgc taattaacga gttgcaaggc attaagaaaa
    21961 cgtatttgta taatttaaat gttttaaaag agaagtcgaa agttgagttg ccgtcaaaaa
    22021 taaaagcaaa gttagacgta gcttttgatc agtttaaaaa agatacattg aaaccaggag
    22081 aaaaggtagc agaagctcag aagaaagttg cagaagctaa gaaaaaagcc gaggatcaaa
    22141 aagaagaaga tcgccgtaac tacccaacca atacttacaa aacgcttgaa cttgacattg
    22201 ctgagtccga tgtgaaagtt aaagaagcgg agcttgaact agtaaacgag gaagctaaac
    22261 ctcgaaacga ggaaaaaatt aagaaagcaa aagcgaaagt tgagagtgaa aaagctgagg
    22321 ctataaggtt agaagaaatc aagacagatc gtgaagaagc taaacgaaaa gcagatgcta
    22381 agttgaagga agctgttgaa aagaatgcag cgaattcaga gcaaggtgaa ccaaagaggc
    22441 gggtaaaacg aggagttctt ggagagccag caacacctga taaaaaagaa aatgatgcga
    22501 agtcttcaga ttctagcgta ggtgaagaaa ctcttccaag cccatccctg aaaccagaaa
    22561 aaaaggttgc agaagctgag aagaaggttg aagaagctaa gaaaaaagcc gaggatcaaa
    22621 aagaagaaga tcgccgtaac tacccaacca atacttacaa aacgcttgaa cttgacattg
    22681 ctgagtccga tgtgaaagtt aaagaagcgg agcttgaact agtaaacgag gaagctaaac
    22741 ctcgaaacga ggaaaaaatt aagaaagcaa aagcgaaagt tg
//
LOCUS       DAVKQL010000034        22706 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVKQL010000034 DAVKQL010000000
VERSION     DAVKQL010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22706
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.034"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            95..166
                     /locus_tag="VW561_001271"
     tRNA            95..166
                     /locus_tag="VW561_001271"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:128..130,aa:Glu,seq:ttc)
     gene            172..245
                     /locus_tag="VW561_001272"
     tRNA            172..245
                     /locus_tag="VW561_001272"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Asn,seq:gtt)
     gene            complement(286..828)
                     /locus_tag="VW561_001273"
     CDS             complement(286..828)
                     /locus_tag="VW561_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEU9943530.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            956..2588
                     /locus_tag="VW561_001274"
                     /pseudo
     CDS             956..2588
                     /locus_tag="VW561_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518194.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
     gene            complement(2618..5170)
                     /locus_tag="VW561_001275"
     CDS             complement(2618..5170)
                     /locus_tag="VW561_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEU9943531.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(5232..6854)
                     /locus_tag="VW561_001276"
     CDS             complement(5232..6854)
                     /locus_tag="VW561_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9943532.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            7060..8085
                     /gene="trpS"
                     /locus_tag="VW561_001277"
     CDS             7060..8085
                     /gene="trpS"
                     /locus_tag="VW561_001277"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEU9943533.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            8237..9715
                     /gene="guaB"
                     /locus_tag="VW561_001278"
     CDS             8237..9715
                     /gene="guaB"
                     /locus_tag="VW561_001278"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEU9943534.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(9766..10863)
                     /gene="recF"
                     /locus_tag="VW561_001279"
     CDS             complement(9766..10863)
                     /gene="recF"
                     /locus_tag="VW561_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEU9943535.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSDQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNVN"
     gene            complement(10866..11234)
                     /gene="yaaA"
                     /locus_tag="VW561_001280"
     CDS             complement(10866..11234)
                     /gene="yaaA"
                     /locus_tag="VW561_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEU9943536.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            11393..12643
                     /locus_tag="VW561_001281"
     CDS             11393..12643
                     /locus_tag="VW561_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU9943537.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIVKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            12640..13923
                     /locus_tag="VW561_001282"
     CDS             12640..13923
                     /locus_tag="VW561_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU9943538.1"
                     /translation="MTKVVFEEKYYPAVKEKVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFCWTSDRFQKCYESGKIDASLSLEVEVTSRFHFVMLTMDTKEPVA
                     LSHQFRKSIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            13965..14786
                     /locus_tag="VW561_001283"
     CDS             13965..14786
                     /locus_tag="VW561_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9943539.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            14797..15342
                     /gene="pgsA"
                     /locus_tag="VW561_001284"
     CDS             14797..15342
                     /gene="pgsA"
                     /locus_tag="VW561_001284"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEU9943540.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            15339..16166
                     /locus_tag="VW561_001285"
     CDS             15339..16166
                     /locus_tag="VW561_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU9943541.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKFLSQNGYNLPENYLTESELEDKLWELL"
     gene            16151..16990
                     /locus_tag="VW561_001286"
     CDS             16151..16990
                     /locus_tag="VW561_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU9943542.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPSILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            16983..17777
                     /locus_tag="VW561_001287"
     CDS             16983..17777
                     /locus_tag="VW561_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9943543.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            17836..18654
                     /gene="mreC"
                     /locus_tag="VW561_001288"
     CDS             17836..18654
                     /gene="mreC"
                     /locus_tag="VW561_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU9943544.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSITIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            18654..19148
                     /gene="mreD"
                     /locus_tag="VW561_001289"
     CDS             18654..19148
                     /gene="mreD"
                     /locus_tag="VW561_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEU9943545.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            19242..20420
                     /locus_tag="VW561_001290"
     CDS             19242..20420
                     /locus_tag="VW561_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEU9943546.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            20644..21423
                     /gene="rpsB"
                     /locus_tag="VW561_001291"
     CDS             20644..21423
                     /gene="rpsB"
                     /locus_tag="VW561_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU9943547.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            21502..22542
                     /locus_tag="VW561_001292"
     CDS             21502..22542
                     /locus_tag="VW561_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEU9943548.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
BASE COUNT         7238 a         3614 c         4652 g         7202 t
ORIGIN      
        1 tagaaaatta aaagatattt tagagaaaaa atctcaaaag tgattgacaa ttagcaagaa
       61 attgatataa tggttatatc tgctacagat agaaggtccg ttggtcaagg ggttaagaca
      121 ccgccttttc acggcggtaa cacgggttcg aatcccgtac ggactatttt atccgccata
      181 gctcagttgg tagtagcgca tgactgttaa tcatgatgtc gtaggttcga gtcctactgg
      241 cggagtatag ataaaaggga acacagctgt gttcctcttt ttgtatcaat ttgtatcacc
      301 aagcattttc ataaggaagt ctgttatttc ttgagaactt tctttttttc catgtgcaat
      361 ccaagtttgg cagacaccaa aaagtgcatg agttagatag atgctactat attctaattc
      421 agtggtattt agattcagtt gcataaatcg cttttgtaaa tctgtactaa gcatgatatg
      481 aagtttattt cgtaagaaat tttggatttc tttagtccca ttttcagaaa gaagggcagc
      541 cagaagtggt tctgactcta gatattcaaa aacttctaaa atagcgtctc ttttgtgatg
      601 agcatgtttt tgaaaaatat attcaaatgt atggaatagc ttgctttgat agtgctcaat
      661 catatcatac ttatccttat agtgagtata gaagctggaa cgactaattc cggctttttc
      721 tactaatttg acagtagaaa ttttatcaaa tggctgttcc atcagtaatt gtaccatagc
      781 attttcaata gttcgctttg tttttaagcg tttgttactt tcttgcatat ttcctccttg
      841 taaacaaatt agactatatg tctaaaaata gattttttat cttgtaattt agatttttta
      901 atgtataatc tattatatca aaattttaga caatatgttt aaaaaaggag aaactatgtt
      961 taaagaatgg aaagcaattt aaaaaaaaac aacctttatt attgtcatga tcgggatttc
     1021 tcttattcca gctctgtaca atatcatatt tttgtcatca atgtgggatc catatgggca
     1081 attgtctgac ttacctgtgg cagttgtaaa taatgataaa gaggcttcct ataatggtaa
     1141 tactatggca ataggaaaag acatggtgtc caatttaaaa gaaaataaaa ccttggattt
     1201 tcattttgta gatgaagagg aaggaaagaa gggattggaa gatggcgatt actatatggt
     1261 agtgacttta ccaagtgatt tatctaaaaa aacaactaca ttatccaata ttcaatcgac
     1321 agcagcttat caatcattga caagtgagca acaaactggg ataagtgatt ctgtatctca
     1381 aaattcaact gatagtattc aatcggctca gtcaattgta gctttagtac aaggtttaca
     1441 gggagtttag aaaacttaca aaatcaatct tctaatcttt cgactttaaa aaatcaagct
     1501 aatcaagtat tacctattac ttctacttct ttgataggat tgtcaagtgg attaacagag
     1561 atacaaggag ctgttactag caaattagtt cctgccagtc agttgattgc atcaggtgta
     1621 aacgcatata ctacaggtgt tgataaagtt tctcagggcg caagtcaact aagtgaaaaa
     1681 aatgccacct tgacaggtag tttggataaa ctagtttcag gctcaaacac cttgacacaa
     1741 aaatcttcta gattgacagc aggatctggg caattagcag acaaatccag tcagttactt
     1801 tcaggtgctt ctccattaga gaatagagct aataaattgg cagatggatc tgggaaacta
     1861 gcagaaggtg gaacaaagtt aacttctgga ttggaagatt tacagacagg acttgcttct
     1921 ttaggacaag gactaggtaa tgctagtgat caactcaaat cagtatcaac agaatctaaa
     1981 aatgcagaga ttttgtcaaa tccactcaat ctttcaaaaa cagacaatga tcaagttcct
     2041 gtaaatggaa tcgcaatagc tccttatatg atatcagttg ctcttttttt tgcagcaata
     2101 tcaacaaata tgatatttgc gaaattgcct tcaggacgtc atccagagag ccgttgggct
     2161 tggttgaaat cttgagctga aataaatggt attatagctg ttttggcagg aattttggta
     2221 tatggaggag ttcagcttat tggtttaact gctaatcatg agatgagaat atttattctc
     2281 atcatcctaa caagtttagt attcatgtct atggtgacca ctttagcaac gtggaatagc
     2341 cgtataggag cttttttctc acttattttg cttttactac agttagcatc aagtgcaggt
     2401 acttatccac ttgctttgac aaatgatttc tttagatcta ttaatccctg gttaccaatg
     2461 agctattcag tttcgggatt acgacaaaca atctctatca acaagtcatt ttcctagctg
     2521 tcatactagc tctatttact ggtttaggta tgctagccta tcaacataag aaaatggaag
     2581 aagattaaaa aaatcgaccg attaactggt cgatttttta tgccttagat gactttcgtc
     2641 tgtgattata gattccaaat agtaagagag aagtaaagga acagattgct ccagtaataa
     2701 aaccattggg aatgaaggaa agtgtaatag ttcctttccc cttgggaatg tcaactttca
     2761 taaatccagt ttgagcttgt ttaatttcta ttttcttacc atcttggtag gcagaccaac
     2821 ctttgtcata aggaatggtg aagaaaatag atgtatcttg ttggacatca tatgtagcaa
     2881 aaaccttgtt tttagaagtt gatactgtga caggttgttc tttaattttt tgaattgcct
     2941 cggtgaaagt tttggtatct aaacgataga aggtaggaga ttcaaatgat acttgtgaat
     3001 ttccagggaa actaacattg atattgaaag tttttttctc tttagtatat cctagattaa
     3061 agaaggagaa gacattatca gttgtaaaag tctttttttc accatttaca agtatgtcaa
     3121 ccttcttttg tttatcgtta gaaaagtgaa ggtttatgaa agagagataa acttggctgt
     3181 tttctggaac ttcaatttga tactggattg ctgcatcttc atttgaagaa cttgtgacac
     3241 taatcaaatc attagtattt tctatttttt ctgttttttc ataaggtatt ggagaaaaat
     3301 aatcaaaatt gacgttagca agttgattta aaaatgaggc ctgattatcc aaggtatgtt
     3361 cattgaactt gacatcattg taaacagatt gactagcaac tgcaatcgga agagagtatt
     3421 gattttcata tagggtaaga ttatcttttt gatagatatc tttaaagcca tacttatcaa
     3481 taggactgtc tgagatattg tactggatac caaataaact atcagccaaa atactattat
     3541 ttgcatatcg gagattgaga ttagtcccag aggatttaaa accaagttta tctaaagtag
     3601 agcttgatga acgatttcga acagatgaaa attgagagat tccattgtag ttgaatttca
     3661 tactgtcatt tcctgtctga gtttgtagtt tttcagtacg agtaaattga tttccaatat
     3721 atgttgagaa agattccata gctgggatat ctcgactata agcacttcga gaagcaaatc
     3781 cccattcctt agcaattccg tccatttgag atgaagcatt taaactcatt tcaaccagta
     3841 taaataaaga gattagaatg gcaaatagat tcacagatat aaactttttg ataactgcaa
     3901 ggagtaaaag agaatagaca accaaaaatt caagagtaag cagaatattc aaatctgtta
     3961 aaaaagaata atgcgatttt agatagatgg tagctaaaaa tcctgctact acaagaaaaa
     4021 gcgaaactaa aaaattccag actttaagtt ctttcagacg ctttaagact tctgctgctg
     4081 tgtaaattaa caaggtagag aaaatccaag cataacgatg taaaaacatg tttggagtat
     4141 gcatgccttg ccaaaataag tcaagagctt ctatgtaaaa gcttgcaatt agaaatgcaa
     4201 agaatattac atatatgagt ttcacgtgaa acttaataga tttcagcgta aaaaataaaa
     4261 tggtcaaaat aaagggaaat agtccaacaa aaatcattgg gatggcccca tactttgttg
     4321 tgtcaaagga accaatgaat tgcttagcaa agagatcaag ataccagcta ctttcagttt
     4381 gaaactttgt aacttcagtc aatttttccc catgtgtctg taaatcaaat agagtgggaa
     4441 gagtcataat caaactagcc ataccagcta aaaaggagat aactatgaaa tcaagaacag
     4501 atgattttcg agtcttaaag tcccacgaaa tttgacagag ataccagaaa ataagaaaca
     4561 atactgtcat atatccaaaa taataatttt gaataaataa gattgacaga cttgtaaagt
     4621 acaataggag tttcttttca gttatcagta gatgtaaacc agttataatt aaaggaatca
     4681 agataaaaac atctagccag gtttttatct ctaattgact gacagtgaaa ctcatcagag
     4741 cataggaagt agataaggct agttttaaag tctgagggat agattgaaat aatttattca
     4801 aactaaaaaa ggttgacaga ccaatcaatc caaattttaa gagagtggtc agatagacag
     4861 catctggcat attcgttaga tcaaaaaagt aaactagagg cgcgagaaaa ctacccaagt
     4921 aataactaga tagggcatag aagtttagcc ctagaccact tgtaaaggtg taaaacagac
     4981 tactatttcc atgtaggata tttcgtaagg ctacatcaaa aataacgtat tgatgaaagc
     5041 catctcctaa tagaggagag ttgtcgctat tccagtagat accttgagat agatatactc
     5101 cagacataat cactacagga atgatgaaag aaataaaata ggttcgatat gtttttaaaa
     5161 atgatttcat gttacctcgt agaatgatag aaaactcagt tggttaaccc aactgagttt
     5221 tgaagtttta tttagtcttt ccaaagttct ttaacttttg cttgtacttc tgcattttct
     5281 aggaattcat cgtaggtttc atcgatacgg tcaatgacgc catttttaga taagacaatg
     5341 atatggttag ccaaagtttg aataaattcg tggtcatggc tagcaaagat gattgattct
     5401 ttaaagtttt tcaatccatc attcaagctt gagatagatt ccaagtccaa gtgatttgtt
     5461 ggatcatcaa gtacaaggac atttgatttt aagagcatga gttttgaaag catgacacga
     5521 actttttctc cccctgacaa gacatttaca ggtttgttaa cttcatctcc agagaagagc
     5581 atacggccga ggaagccacg taggaaagta ttgtcatctt cttctttact tgcgaattga
     5641 cgcaaccagt caagaattga ttctcctcct gcaaaatcag ctgagttatc ttttggtagg
     5701 taagattgac tagttgtaac tccccacttg acagttcctt catagtcaat atctcccatg
     5761 attgcacgaa ttaatgcagt cgtttgaata tcattttgtc caataagtgc tgtcttatca
     5821 tctggacgca agatgaaact aatattatcc aagatagttt caccatcaat ctttacagtt
     5881 agattttcta ctgtcaagag atcattacca atctcacgtt ccgctttaaa gttgataaat
     5941 ggatatttac gactagatgg cacaatctct tctagctcaa tcttatcaag cattttctta
     6001 cgtgatgttg cctgccttga cttagaagca ttggcagaga aacgagcaac gaattcttgc
     6061 aattgtttaa ttttttcttc tgctttagca ttacggtctg ctagcaattt agcagcaagc
     6121 tcagaagatt ccttccagaa gtcgtagttt ccgacataga gtttgatttt tccaaagtca
     6181 aggtcggcca tgtgagtaca aactttgttt aagaagtgac ggtcgtggga tactacgata
     6241 actgtgttat caaagtcaat taagaagtct tctaaccaag taatcgattg gatatccaaa
     6301 ccgttagtag gctcgtccaa gagaagaaca tctggtttac caaaaagtgc tttggcgagg
     6361 agaaccttta ctttttcacc gttggccaat tcgctcatgt tttggtagtg taattcttct
     6421 ggaatgttta ggttttgaag tagttgagag gcttcactct ctgcttccca acctccaagt
     6481 tcggcaaact ctccttcgag ttcggcagca cgaaccccgt cctcgtctga gaaatcttcc
     6541 ttcatgtaga tagcatcttt ctctttcatg atgctataaa gtttttcatt tcccatgata
     6601 acgacatcaa tggcacgttc atcttcgtag tcaaagtgat tttgacgaag aacagagaga
     6661 cgttcatctg gaccaagaga gatgtgacca gtagtaggtt cgatatctcc agctaaaatt
     6721 tttaaaaagg ttgattttcc ggcaccatta gcaccgatta atccgtaagt atttccttct
     6781 gtaaatttga tattgacatc atcaaaaagt ttgcgatcac taaaacgtag tgaaacatca
     6841 gatactgtaa gcaatatttt tctcctatat gtgtaatata tttattctac tagaaaatac
     6901 agaaatattc aaatttttat ttgtcaattt tgtgtaaatt atatttacag tatcctttac
     6961 acaaatctgt aaaaagcaag gctgatttat tttgataaat tacggttatt tcattaaaaa
     7021 aatgctataa ttgaaaggac tatatcgaag gagaacaaaa tgactaaacc cattatttta
     7081 acaggagacc gtccaacagg aaaattgcat attggacatt atgttggaag tctcaaaaat
     7141 cgagtattat tacaggaaga ggataagtat gatatgtttg tgttcttggc tgaccaacaa
     7201 gccttgacag atcatgccaa agatcctcaa accattgtag agtctatcgg aaatgtggct
     7261 ttggattatc ttgcagttgg attggatcca aataagtcaa ctatttttat tcaaagccag
     7321 attccagagt tggctgagtt gtctatgtat tatatgaatc tagtttcgtt agcacgtttg
     7381 gagcgaaatc caacagtcaa gacagagatt tctcagaaag gatttggaga aagcattccg
     7441 acaggattct tggtctatcc aatcgctcaa gcagctgata tcacagcttt caaggctaat
     7501 tatgttcctg ttgggacaga tcagaaacca atgattgagc aaactcgtga aattgttcgt
     7561 tcttttaaca atgcatataa ctgtgatgtc ttggtagagc cggaaggtat ttatccagaa
     7621 aatgagagag cagggcgttt gcctggttta gatggaaatg ctaaaatgtc taaatcacta
     7681 aataatggta tttatttagc tgatgatgcg gatactttgc gtaaaaaagt aatgagtatg
     7741 tatacagatc cagatcatat ccgcgttgag gatccaggta agattgaggg aaatatggtt
     7801 ttccattatc tagatgtttt tggtcgtcca gaagatgctc aagaaattgc tgatatgaaa
     7861 gaacgttatc aacgaggtgg tcttggtgat gtgaagacca agcgttatct acttgaaata
     7921 ttagaacgtg aactgggtcc tattcgtgag cgccgtattg aatttgctaa ggatatggga
     7981 gaagtttata atatgattca aaaaggtagt gaaagagcgc gtgaagttgc gggtcaaacc
     8041 ctatctgagg taaaaggggc aatgggactt cattacttta actaagttta ttttacaaga
     8101 aactatcatt tctatctcga agaaaagata gttttttatt tacccctgta acatttgaca
     8161 aatttgtata gaatggtatg atagatacaa tataaaaaga gtcaagctca aaaagaaaga
     8221 aaagaggaaa cttcgaatgt ctaattggga cactaaattt ttgaaaaaag gttttacctt
     8281 tgatgatgta ttgcttattc cagctgaaag tcatgtgttg cctaacgatg cagatttaac
     8341 aactaaattg gcagataatt tgactttaaa tatcccaatt attaccgctg ccatggacac
     8401 agttacagag agtcaaatgg ccattgctat tgctcgtgca ggtggtctcg gagttatcca
     8461 taaaaacatg tcaattgctc aacaagcaga cgaggttcgt aaggtaaaac gttctgaaaa
     8521 tggagttatt attgatccgt tcttcttgac gcctgaacat acaattgctg aagcagatga
     8581 gcttatgggt cgttaccgca tcagtggtgt tccagttgtt gaaacacttg aaaatcgtaa
     8641 attggttggt attttgacaa accgagatct tcgttttatt tcagattata atcaaccaat
     8701 ttcaaaccat atgactagtg aaaatcttgt tactgctcct gtgggtacgg atcttgcaac
     8761 ggctgagagt attcttcaag agcatcgtat tgaaaaactt ccgttggtcg atgaagaagg
     8821 cagtctttct ggtttgatca ctatcaaaga tattgaaaaa gttattgagt ttccaaatgc
     8881 ggctaaagat gagtttggtc gtcttctagt tgcaggtgca gtaggtgtta cttcagatac
     8941 atttgaacgt gcagaggctc tttttgaggc aggagcggat gcgattgtta ttgatactgc
     9001 acatggtcat tctgcaggtg tcttgcgtaa aattgccgag attcgtgctc atttcccaga
     9061 tcggactttg attgctggaa atattgctac tgctgaaggt gcacgtgccc tttatgaagc
     9121 gggtgtagac gttgttaagg ttggtattgg accaggttct atctgtacta ctcgtgtgat
     9181 tgctggtgtt ggtgttccgc aagtaacagc tatctacgat gctgcagctg ttgcgcgcga
     9241 atatggtaaa acgattattg ctgacggtgg gatcaagtat tctggagata ttgtaaaagc
     9301 acttgctgca ggtggaaatg ctgttatgct tggatctatg tttgctggaa ctgatgaagc
     9361 tccaggcgaa actgaaatct tccaaggacg taaattcaag acttaccgtg gtatgggatc
     9421 aattgctgct atgaagaaag gttcaagcga ccgttatttc caaggttctg tcaatgaagc
     9481 aaacaagctt gttccagaag gaattgaagg tcgtgttgct tataaaggag cggcagctga
     9541 tattgttttc caaatgattg gtggtattcg ctctggtatg ggttactgtg gtgcagctaa
     9601 ccttaaagaa ctacacgata atgctcaatt tattgaaatg tctggtgctg gtttgaaaga
     9661 aagccatcct catgatgtgc aaattactaa tgaggcacca aattattcta tgtaaaaaac
     9721 aatgaaaaga actccagtga aaacaggagt tcttttacaa tgttgtcaat ttacatttac
     9781 agcagcttta ccatcctgaa tagtgaagat acttagattt tctggcagat tttgaagatg
     9841 gtctaagctt gttgttgtga taaaggtttg gattgattga gaaatcgttt ctaataattt
     9901 taactgtcta gtgttgtcaa gttcactcat cacatcgtca agcagtaata tcggagattc
     9961 tgtggtaatg ctttccatta attcgatttc tgctaatttt atcgagagga cgagactacg
    10021 atgttgacct tggcttccga aactagcatc catcccattt atataaaaag aaatgtcatc
    10081 tcgatgagga ccgacaccag tattcttttt aaataaatct ctggatctac ttttttctaa
    10141 agcaattttg aaagattcgg ataagttttg tttgtcagtt atattgacag aagattgata
    10201 ggatattgac aactcttcga tctgattaga gagttcaaaa tgtttcttac gcccaaatga
    10261 ttctagtttt tttatgaaat ctaagcggtg attcattaca cgacatccat aatcaactag
    10321 ctgatcatct aacacagaaa ggaatgtttc atctattttt tgatctgatt ttaggtaagt
    10381 gtttctttgc tttaggatgt ggttataatt ggttaagtca gataagtaga ttggcttaat
    10441 ttgcccaagt tccatatcaa tgaattttcg tcgaatcgaa ggtgctcctt taattagttg
    10501 taaatcttca ggagcaaata agacaacatt catgtgtcct acataatctg aaaggcgtgc
    10561 ctgttttaag tgattaactt ttgttacacg gcctttttgt gttagttcga tttctagggg
    10621 aatggatcca gtttttttct gaacgagacc tgaaagatga agttgttcct catcaaaatg
    10681 aatgagattt ttatctgttc gagttcgatg actacgcgtt aaggctaaaa aatagatagc
    10741 ctctaacatg tttgtttttc cttgtgcatt acgtcccaaa aagacattca atttaggatt
    10801 aaagtctatt ttcgtttctt tgtaattacg aaaagtcttg agagatagat gttgtagcca
    10861 catgattatc taccagggaa tcgtggagct tgttggcttt taggtgatga agcaggtttc
    10921 gatttgtctt ttttaactcc cttattcatc tttttgacaa gtttagcgat ccgttctttt
    10981 tcaactttat cagcttggta ttcatcttgt tcttcagaag taggttgtgt caacaagatg
    11041 tcaatgttca tgtcagggat gtcaacttta tcgccaatac gaagtttttt tccacgacga
    11101 ctctctaatt ccctattaaa gtaaacagaa tgttcagaga gaaatgattt aatagctcct
    11161 ccgctatgtg taattccaag ttctttgagt agtgcttgga gggtaataaa ttcttcaaat
    11221 aatttgtatt ccataattca cctcaatctt tggtattata ccatattttc ctaaaaatag
    11281 tgagagagac agagagaaat gtaagcgatt tttatttcaa aagtgttaca gggaataaga
    11341 aaatttcagg ttttcgtgat ataatagaag tctgtatata aggaggtaaa tcatggagtt
    11401 agtgcatgga atttcaacac attttatcca atcaaaaaag tttaaaacaa acaaaattac
    11461 cgtgcgtttt accgctccat tatcccttga tacgattgca ggtcacatgt tgagtgcaag
    11521 tatgctagag actgctaatc agatgtaccc cacttctcaa gatttgagga gacacttggc
    11581 cagtctatac ggtacagata tgtcaaccaa ttgtttcaga agagggcaaa gccacattat
    11641 agaattgaca tttacctatg ttcgtgatga gtttttaagt aggaaaaacg tgctaacctc
    11701 tcagattttg gaacttgtaa aagaaactct tttttcaccc gtagtagttg ataatgggtt
    11761 tgatccggcc ttatttgaaa ttgagaaaaa acaattgcta gcaagtttag cagctgatat
    11821 ggatgattct ttttattttg cacataaaga attggataaa ttgttttttc atgatgaacg
    11881 tcttcaattg gaatatagtg atttacgaaa tcgtatttta gctgaaactc cacaaagttc
    11941 ttattcttgt ttccaagaat ttttagccaa tgatcgaata gatttctttt tcctaggtga
    12001 ttttaatgag gttgaaattc aaaatgtatt agaatcattt ggctttaaag gtcgaaaagg
    12061 agatgtgaag gttcagtatt gtcaacctta ttctaatatc cttcaggaag gtatggttcg
    12121 gaaaaatgtg ggacaatcca ttttggaatt aggttatcat taccgttcta aatatggtga
    12181 tgagcaacat ttacccatga ttgtaatgaa tggtttactt ggtggatttg ctcattctaa
    12241 gctctttaca aatgtccgtg aaaatgctgg attagcttat accatttcaa gtgagcttga
    12301 tttatttagt ggattcttga ggatgtatgc tggtatcaat cgagaaaatc gtaaccaggc
    12361 tcgtaaaatg atgaataatc aactgcttga tttaaaaaaa ggttatttta cagagtttga
    12421 gttaaatcag accaaggaaa tgattcgttg gtcgttgtta ctttctcaag ataatcaatc
    12481 ttcattgatt gaacgtgctt atcaaaatgc cttatttgga aaatcttcag cagactttaa
    12541 aagttggatt gtaaagcttg aacagattga caaagatgct atttgtagag tagctaataa
    12601 tgtgaaacta caagcgattt actttatgga aggaatagaa tgacaaaggt tgtttttgaa
    12661 gaaaaatact atccagctgt aaaagaaaag gtttatcgaa ctcgtttggc caatggattg
    12721 acagttgctc ttttgcctaa aaaggaattt aaagaggttt acgggagtgt cactgtacag
    12781 tttggttcgg tagatacgtt tgtcacagaa gttgacggag atgtaaaaca atatcctgga
    12841 ggaattgctc attttcttga acataaatta tttgagagag aagattctag tgatttgatg
    12901 tcggctttta cgagtctagg tgcagatagt aatgccttta caagctttac aaaaacaaac
    12961 tatctttttt cagcaacgga ttatttttta gaaaatttag atttacttga tgaattggta
    13021 acatcagcac actttactga agcttccatt ctgacagagc aggatattat tcagcaagaa
    13081 cgagaaatgt accaagatga tccagattcg tgtttattct tttcaacttt agcgaatttg
    13141 tatcctggta cacctttagc aactgatata gttggaagtg aggagtccat ttcccaaatc
    13201 aatctaacta atttgcaaga aaattttaca aagttttaca aacctgtaaa catgtctctg
    13261 tttttagttg gtaattttga tgtggagcga gtacaggact attttgaaag caaagaactg
    13321 aaagattcag attttcagga agtagcaaga gaaaagttgt ttttacagcc tgtaaagcca
    13381 acagatagta tgagaatgga agtatcttct cccaaactag cgattggagt tagaggtaag
    13441 cgagaagttt ctgaagcgga ttgctatcga catcatattt tattaaagtt attgtttgca
    13501 atgatgtttt gttggacttc ggatcgtttt caaaaatgtt atgaatcagg taaaattgat
    13561 gcatccttat ctctggaagt tgaagtaaca agtcgctttc attttgtcat gttgacaatg
    13621 gatacgaaag agccagttgc tttgtctcat caatttagga agtctattcg taattttaca
    13681 aaggatttag atattacaga ggaacattta gatattatca aaagagagat gtttggcgaa
    13741 tttttcagta gcatgaactc tcttgaattt attgcaacgc aatatgatgc ttttgaaaat
    13801 ggtgagacaa tttttgattt gccgaaaatt ttacaggaaa ttactttaga ggatgtcctt
    13861 gatgctggac atcatttaat agatgatggt gacatagttg attttacaat attcccatcg
    13921 tagtaaccta ttataataga cactagaaag aagggatgac aagtatgaga aaaaaaacaa
    13981 ttggagaggt tttacgatta gctagaatca atcagggatt gagtttagat gaattgcaga
    14041 aaaagacaga aatccagtta gatatgttgg aagcaatgga agcagacgat ttcgatcaac
    14101 ttccaagtcc tttttacacg cgttctttct tgaaaaaata tgcatgggct gttgagttag
    14161 atgaccaaat tgttttggat gcttatgatt ctgggagtat gattacttat gaggaagtag
    14221 atgttgatga agatgagttg acaggtcgta gacgttcaag taagaaaaag aagaaaaaaa
    14281 catcattttt acctttattt tattttatcc tttttgcttt atcgatttta atttttgtga
    14341 cttattatgt ttggaactat attcaaactc aaccagagga gccttctctt tctaattaca
    14401 gtgtggttca atcaacaagt tcaactagct ctgttcccca ctcctcaagt agtagttctt
    14461 ctagtataga atcagctata agtgtatcag gcgaaggaaa tcatgtagaa atcgcttata
    14521 agacaagtaa ggaaacagtt aaattgcaat tggcagtttc agatgttaca agttgggtca
    14581 gtgtttcaga aagcgaactt gagggcggtg taaccttatc gccaaagaag aaaagtgcag
    14641 aagcaacagt tgcaactaaa agtcctgtaa caattacgtt aggtgttgta aaaggtgttg
    14701 atttgacagt agataatcag actgttgatt tatcgaaatt aacagctcag actggacaaa
    14761 tcactgtaac ctttactaaa aattaaggaa aaacgaatga aaaaagaaca aattcccaat
    14821 ctcttaacaa taggtcgaat tctctttata cctattttta tctttatttt aacgatagga
    14881 aattcgatag agagtcatat agttgcagct attatctttg ctgttgccag tattaccgac
    14941 tatttagatg gatatttagc tcgtaaatgg aatgtggtca gtaattttgg taaatttgca
    15001 gatcctatgg cggataagtt actagttatg tcggctttta ttatgttgat tgagttaggt
    15061 atggctccgg cttggattgt tgcagtgatt atctgtcgtg agttagctgt gacaggttta
    15121 aggcttttat tggttgaaac tggtggaaca attttagcag cagcaatgcc tggaaaaatt
    15181 aaaactttta gtcagatgtt tgccattatt ttcttgctat tacattggac tttgcttggt
    15241 caagttctac tttatgtagc cttatttttc actatctact ctggctatga ctatttcaag
    15301 ggtagtgcct atgtatttaa agggacattt ggttcgaaat gaaatcaata attgatgtaa
    15361 aaaatctttc ttttcgctat aaagaaaatc agaactacta cgatgtgaag gatattacgt
    15421 ttcacgtgaa acgtggagaa tggctttcga ttgtagggca taatggtagt ggtaaatcaa
    15481 cgacggttcg attaattgat ggcttactgg aagcagaatc cggagagatt gtaattgatg
    15541 gccaacgttt gactgaggaa aatgtttgga atatacgtcg tcaaatcggt atggtttttc
    15601 aaaatccaga caatcaattt gttggagcga ctgttgaaga tgatgttgct tttggtttgg
    15661 aaaatcaggg actttctcgt caagaaatga aaaagagagt ggaagaagct ctggctttag
    15721 ttggcatgtt ggactttaaa aagagagagc cagcgcgtct atcaggtggc caaaagcaac
    15781 gtgtggccat tgcaggtgtt gtagccctaa gaccagctat tttaatctta gatgaagcaa
    15841 cgagtatgtt ggatcctgag gggcgtagag aacttattgg gacagtaaaa ggaattcgaa
    15901 aagactatga tatgacggtc atttctatta cccatgattt ggaagaagtc gccatgagtg
    15961 atcgcgtatt ggtcatgaaa aaaggggaaa ttgaatcaac tagtagtcca agggagcttt
    16021 tctctcgaaa tgatttagat caaattggat tagacgatcc ttttgctaat caattaaaaa
    16081 aatttttgag ccagaatggc tataatttac ctgaaaatta tttgacagaa agtgagctag
    16141 aggataagct atgggaattg ctctagaaaa tgtgaatttt acatatcaag aaggtactcc
    16201 cttagcttca gcagctttgt cggatgtttc tttgacgatt gaagatggct cttatacagc
    16261 tttaattggg cacacaggta gtggtaaatc aactatttta caactcttaa atggtttatt
    16321 ggtgccaagt caagggagtg tgagggtttt tgatacctta atcacctcga cttctaaaaa
    16381 taaagatatt cgtcaaatta gaaaacaggt tggcttggta tttcagtttg ctgaaaatca
    16441 gatttttgaa gaaacggttt tgaaggacgt tgcttttgga ccgcaaaatt ttggagtttc
    16501 tgaagaagat gctgtgaaga ctgcgcgtga gaaactggct ctggttggaa ttgatgaatc
    16561 actttttgat cgtagtccgt ttgagctgtc agggggacaa atgagacgtg ttgccattgc
    16621 aggcatactt gccatggagc catctatatt agtcttagat gagccaacag ctggtctaga
    16681 tcctctaggg agaaaagagt tgatgaccct gttcaaaaaa ctccaccagt cagggatgac
    16741 catcgtcttg gtaacgcatt tgatggatga tgttgctgaa tatgctaatc aagtctatgt
    16801 aatggaaaag ggacgtttag taaagggggg caaaccaagt gatgtctttc aagacgttgt
    16861 ttttatggaa gaagttcagt tgggagtacc taaaattacg gccttttgta aacgattggc
    16921 tgatagaggc gtgtcattta aacgattacc gattaagata gaggagttca aggagtcgct
    16981 aaatggatag tatgattttg gggcgttata tcccagggga ttcgattgtt caccgattgg
    17041 atccacgtag caaattgctt gctatgatgc tactgatttt gattgtgttt tgggctaata
    17101 accccttgac gaatctgatt ctttttatag cgacagggat atttattgcc ttgtcaggag
    17161 tatctctttc attttttatt cagggcttga agtctatgtt tttcttgatt gccttcacaa
    17221 ctatttttca actatttttc atttctaatg ggaatgtttt atttgagttt tcgtttgtga
    17281 gaatcacgga ttatgctttg caacaagctg ggattatttt ttgtcgcttt gtattgatta
    17341 ttttcttttc aactttgtta accttaacaa ccatgccttt aagtttggca tcagctgtcg
    17401 aagctttatt agcaccttta aagcgtgtga aagttccagt tcatgaaatt ggattgatgc
    17461 tgtctatgag tttgcgtttt gtcccaacct tgatggatga tacgactcgg attatgaatg
    17521 cacagaaagc tcgtggagtg gattttggag aaggaagcat cgttcaaaaa gtaaaggcga
    17581 tgattcccat tttgattcct ctttttgcga caagtttaaa acgtgcagat tccttggcta
    17641 tcgctatgga agcgcgtggc tatcagggtg gaaaaggcag aagtcaatac cgacaattga
    17701 aatggactct aaaggatacg ctgaccattc ttgttattct cgtacttggt tgttgtttat
    17761 ttttcttaaa atcttagtag ctttcaggaa ttgataaaaa gttactgtaa cagtttttga
    17821 tagaaaggta gggatatgaa ccgttttaaa aaatcaaaat atgtcattat tgtttttgtc
    17881 actgttctgc ttgtgtcagc tctcttagcg acgacttatt caagtacaat tgtgacaaaa
    17941 ttaggagatg gaatctcatt ggttgataga gttgtacaaa aaccttttca gtggtttgat
    18001 tctgtcaaat cagatttggc tcatttgaca cgaacatata atgaaaatga aagtttgaag
    18061 aaacagcttt accaattaga agttaaatca aatgaggtgg aaagtttaaa gacagaaaat
    18121 gaacaactgc gccaattgct tgatatgaag tctaaattgc aagccacaaa gactttagca
    18181 gcagatgtta ttatgcgttc tccggtatct tggaagcagg agttgacctt agatgcaggt
    18241 agatcaaaag gtgcttctga gaacatgtta gctattgcaa atggtggctt gattgggagt
    18301 gtttcaaaag tagaggagaa ctctactata gtcaaccttc tgacaaatac ggaaaatgct
    18361 gataagattt ctgttaaaat tcaacatggc tctattacaa tttatggaat tattattggc
    18421 tatgacaagg aaaatgacgt tcttaaaatt agccaattaa atagtaatag cgatattagt
    18481 gcgggagata aggtgactac tggtggatta ggaaacttta acgttgctga tattcctgtt
    18541 ggtgaagtgg ttgccacaac gcatagtaca gactatttga cacgagaagt aactgttaaa
    18601 ttgagtgcag atactcataa tgtagatgtg atagaattag tggggaattc ataatgagac
    18661 agttgaagcg agttggagta tttttattgc ttcctttctt tgttctaatt gacgcccata
    18721 ttagccagct tctgggctca tttttccccc atgtacattt ggctagtcat tttctttttc
    18781 tatttctttt atttgagacg atagaagtat cagagtatct ctacctagtc tattgttttg
    18841 ttataggctt ggtttatgat gtttactttt tccatctaat agggattaca actctcttat
    18901 ttatcttatt gggagccttc cttcataaat tgaatagtgt tattttgttg aatcgttgga
    18961 caagaatgct agctatgatt gtgctgacat tcctgtttga aatgggtagt tatcttttgg
    19021 cttttatggt agggttgaca gtagatagca tgtcgatttt tatagtctat agcttggtac
    19081 cgacgatgat tttaaatttt ttatggatta ctgtttttca atttattttt gaaaaatatt
    19141 atctataaga acgacatata aatgtaacaa aggcgtaata tttattaggc ctttttttgg
    19201 tatactagta ttgtctttaa aagaaggagt atctacgtaa tatgaagaaa aaaatcttag
    19261 cgtcactttt attaagtaca gtaatggttt ctcaagtagc tgttttaaca actgcgcatg
    19321 cagaaacgac tgatgacaaa attgctgctc aagataataa aattagtaac ttaacagcac
    19381 aacaacaaga agcccaaaaa caagttgacc aaattcagga gcaagtatca gctattcaag
    19441 ctgagcagtc taacttgcaa gctgaaaatg atagattaca agcagaatct aagaaactcg
    19501 agggtgagat tacagaactt tctaaaaaca ttgtttctcg taaccaatcg ttggaaaaac
    19561 aagctcgtag tgctcaaaca aatggagccg taactagcta tatcaatacc attgtaaact
    19621 caaaatcaat tacagaagct atttcacgtg ttgctgcaat gagtgaaatc gtatctgcaa
    19681 acaacaaaat gttagaacaa caaaaggcag ataaaaaagc tatttctgaa aaacaagtag
    19741 caaataatga tgctatcaat actgtaattg ctaatcaaca aaaattggct gatgatgctc
    19801 aagcattgac tacgaaacag gcagaactaa aagctgctga attaagtctt gctgctgaga
    19861 aagcgacagc tgaaggggaa aaagcaagtc tattagagca aaaagcagca gctgaggcag
    19921 aggctcgtgc agctgcggta gcagaagcag cttataaaga aaaacgagct agccaacaac
    19981 aatcagtact tgcttcagca aacactaact taacagctca agtgcaagca gtatctgaat
    20041 ctgcagcagc acctgtccgt gcaaaagttc gtccaacata cagtacaaac gcttcaagtt
    20101 atccaattgg agaatgtaca tggggagtaa aaacattggc accttgggct ggagactact
    20161 ggggtaatgg agcacagtgg gctacaagtg cagcagcagc aggtttccgt acaggttcaa
    20221 cacctcaagt tggagcaatt gcatgttgga atgatggtgg atatggtcac gtagcggttg
    20281 ttacagctgt tgaatcaaca acacgtatcc aagtatcaga atcaaattat gcaggtaatc
    20341 gtacaattgg aaatcaccgt ggatggttca atccaacaac aacttctgaa ggttttgtta
    20401 catatattta tgcagattaa tttacagagg gactcgaata gagccctctt ttcaggtttt
    20461 accgtgacaa tccctattaa aaattatatc aaaatagctt gaaaatattg gaaaagtatg
    20521 gtagaatgaa aattgtcgtg tgaacgataa tactcattct tgatgaattg tgaagcagtt
    20581 gcccttgggt cgttttgcga gttgaagtca agaagaggaa aaaaacaaaa aggagaaata
    20641 ctcatggcag taatttcaat gaaacaactt cttgaggctg gtgtacactt tggtcaccaa
    20701 actcgtcgct ggaatcctaa gatggctaag tacatcttta ctgaacgtaa cggaatccac
    20761 gttatcgact tgcaacaaac tgtaaaatac gctgaccaag catacgactt catgcgtgat
    20821 gcagcagcta acgatgcagt tgtattgttc gttggtacta agaaacaagc agctgatgca
    20881 gttgctgaag aagcagtacg ttcaggtcaa tacttcatca accaccgttg gttgggtgga
    20941 actcttacaa actggggaac aatccaaaaa cgtatcgctc gtttgaaaga aattaaacgt
    21001 atggaagaag atggaacttt cgaagttctt cctaagaaag aagttgcact tcttaacaaa
    21061 caacgtgcgc gtcttgaaaa attcttgggc ggtatcgaag atatgcctcg tatcccagat
    21121 gtgatgtacg tagttgaccc acataaagag caaatcgctg ttaaagaagc taaaaaattg
    21181 ggaatcccag ttgtagcgat ggttgacacc aatactgatc cagatgatat cgatgtaatc
    21241 atcccagcta acgatgacgc tatccgtgct gttaaattga tcacagctaa attggctgac
    21301 gctattatcg aaggacgtca aggtgaggat gcagtagcag ttgaagcaga atttgcagct
    21361 tcagaaactc aagcagattc aattgaagaa atcgttgaag ttgtagaagg tgacaacgct
    21421 taatttatac aaatagtaat tacctaggag ggcggggctt agcccggctc tcctattttc
    21481 aaaaaatata ggagaattaa aatggcagaa attacagcta aacttgtaaa agagttgcgt
    21541 gaaaaatctg gtgccggtgt tatggacgct aaaaaagcgc ttgtagaaac agacggtgac
    21601 atcgaaaaag cgattgaatt gcttcgtgaa aaaggtatgg ctaaggcagc taagaaagct
    21661 gaccgtgttg ctgcagaagg tttgactggt gtttatgtta acggtaatgt tgcagcagtt
    21721 attgaagtaa acgctgaaac tgacttcgtt gcaaaaaacg ctcaattcgt tgaattggta
    21781 aatactacag ctaaagtcat tgctgaagga aaacctgcta acaatgaaga agctcttgct
    21841 ttgataatgc cttcaggtga aactcttgaa gctgcatacg tatctgcaac agcaactatc
    21901 ggagagaaaa tctcattccg tcgctttgca ttgattgaaa aaacagacgc acaacacttt
    21961 ggagcatacc aacataacgg tggacgtatc ggtgttattt cagttgttga aggtggagac
    22021 gaagcacttg ctaaacaatt gtcaatgcac atcgcagcga tgaaaccaac agttctttct
    22081 tacaaagaat tggatgagca attcgttaaa gatgagttgg cacaattgaa tcacgttatc
    22141 gaccaagaca acgaaagccg tgcaatggtt aataaaccag ctcttccaca cttgaagtat
    22201 ggatcaaaag ctcaattaac tgatgatgtt attgctcaag ctgaagctga catcaaagct
    22261 gaattggctg cagaaggcaa accagaaaaa atctgggaca aaattattcc aggtaaaatg
    22321 gatcgcttca tgcttgataa cactaaagtt gaccaagctt acacacttct tgcacaagtt
    22381 tacatcatgg atgacagcaa gacagttgaa gcataccttg aatcagttaa cgcttcggta
    22441 gttgagtttg ctcgctttga agttggtgaa ggtatcgaga aagctgcaaa cgacttcgaa
    22501 gctgaagttg cagctacaat ggcagcagcc ttgaataact aataatagaa aggaagcaaa
    22561 ttgcttcctt ttttgtatag ggcttagatt tttcctgaaa ttgttttttc cccagtctca
    22621 ctcgattaga ataattaaaa caaaaagccc tataataagg gctaagggca tttaggagac
    22681 tatacttcaa attcatagta agcgcg
//
LOCUS       DAVKQL010000035        22703 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVKQL010000035 DAVKQL010000000
VERSION     DAVKQL010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22703
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.035"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(157..381)
                     /locus_tag="VW561_001293"
     CDS             complement(157..381)
                     /locus_tag="VW561_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory protein Asp5"
                     /protein_id="HEU9943549.1"
                     /translation="MIEILIVLAIILSLALIVLVTIQPRQNQLFSMDATSNIGKPSYW
                     QSNTLVKVLTLLVSLALFVLLLTFMVITYK"
     gene            complement(386..565)
                     /gene="asp4"
                     /locus_tag="VW561_001294"
     CDS             complement(386..565)
                     /gene="asp4"
                     /locus_tag="VW561_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory system protein Asp4"
                     /protein_id="HEU9943550.1"
                     /translation="MSEEDLFYKDVEGRMEELKQKPIKKEKETRGEKISKTFSLLLGL
                     MILIGLLFTLLGILR"
     gene            complement(567..1904)
                     /gene="gtfB"
                     /locus_tag="VW561_001295"
     CDS             complement(567..1904)
                     /gene="gtfB"
                     /locus_tag="VW561_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HEU9943551.1"
                     /translation="MIELYDSYSQESRDLHESLGATGLSQLGVVIDADGFLPDGLLSP
                     FTYYLGYEDGKPLYFNQVPVSDFWEILGDNQSACIEDVTQERAVIHYADGMQARLVKQ
                     VDWKDLEGRVRQVDHYNRFGACFATTTYSADSEPIMTVYQDVNGQQVLLENHVTGDIL
                     LTLPGQSMRYFANKVEFITFFLQDLEIDTSQLIFNTLATPFLVSFHYPDKSGSDVLVW
                     QEPLYDAIPGNMQLILESDNVRTKKIIIPNKTTYERALELTDEKYHDQFVHLGYHYQF
                     KRDNFLRRDALILTNSDQIEQVEAIAGALPDVTFRIAAVTEMSSKLLDMLRYPNVVLY
                     QNASPQKIQELYQLSDIYLDINHSNELLQAVRQAFEHNLLILGFNQTVHNRLYITPDH
                     LFESSEVAALVETIKLALSDVDQMRQALGKQGQHANYVDLVRYQETMQTVLGG"
     gene            complement(1897..3408)
                     /gene="gtfA"
                     /locus_tag="VW561_001296"
     CDS             complement(1897..3408)
                     /gene="gtfA"
                     /locus_tag="VW561_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HEU9943552.1"
                     /translation="MTIYNINLGIGWASSGVEYAQAYRAGVFRKLNLSSKFIFTDMIL
                     ADNIQHLTANIGFDDNQVIWLYNHFTDIKIAPTSVTVDDVLAYFGGEESHREKNGKVL
                     RVFFFDQDKFVTCYLVDENKDLVQHAEYVFKGNLIRKDYFSYTRYCSEYFAPKDNVAV
                     LYQRTFYNEDGTPVYDILMNQGKEEVYHFKDKIFYGKQAFVRAFMKSLNLNKSDLVIL
                     DRETGIGQVVFEEAQTAHLAVVVHAEHYSENATNEDYILWNNYYDYQFTNADKVDFFI
                     VSTDRQNEVLQEQFAKYTQHQPKIVTIPVGSIDSLTDSSQGRKPFSLITASRLAKEKH
                     IDWLVKAVIEAHKELPELTFDIYGSGGEDSLLRDIIANHQAEDYIQLKGHAELSQIYS
                     QYEVYLTASTSEGFGLTLMEAIGSGLPLIGFDVPYGNQTFIEDGQNGYLIPSSSDHVE
                     DQIKQAYAAKICQLYQENRLEAMRAYSYQIAEGFLTKEILEKWKKTVEEVLHD"
     gene            complement(3420..5792)
                     /gene="secA2"
                     /locus_tag="VW561_001297"
     CDS             complement(3420..5792)
                     /gene="secA2"
                     /locus_tag="VW561_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HEU9943553.1"
                     /translation="MFRRLGQDFQLRKVKKILKQINALKGKMSSLSDQELVAKTVEFR
                     QRLSEGESLDDILVEAFAVVREADKRILGMFPYDVQVMGAIVMHYGNVAEMNTGEGKT
                     LTATMPVYLNAFSGEGVMVVTPNEYLSKRDAEEMGQVYRFLGLTIGVPFTEDPKKEMK
                     AEEKKLIYASDIIYTTNSNLGFDYLNDNLASNEEGKFLRPFNYVIIDEIDDILLDSAQ
                     TPLIIAGSPRVQSNYYAIINTLVTTLVEGEDYIFKEEKEEVWLTTKGAKSAENFLGID
                     NLYKEEHASFARHLVYAIRAHKLFTKDKDYIIRGNEMVLVDKGTGRLMEMTKLQGGLH
                     QAIEAKEHVKLSPETRAMASITYQSLFKMFNKISGMTGTGKVAEKEFIETYNMSVVRI
                     PTNRPRQRIDYPDNLYITLPEKVYASLEYIKQYHAKGNPLLVFVGSVEMSQLYSSLLF
                     REGIAHNVLNANNAAREAQIISESGQMGAVTVATSMAGRGTDIKLGKGVAELGGLIVI
                     GTERMESQRIDLQIRGRSGRQGDPGMSKFFVSLEDDVIKKFGPSWVHKKYKDYQVQDM
                     TQPEVLKGRKYRKLVEKAQHASDSAGRSARRQTLEYAESMNIQRDMIYKERNRLIDGS
                     RDLEDVVVDIIERYTEEVAADHYASRELLFHFIVTNISFHVKEVPDYIDVTDKTAVRS
                     FMKQVIDKELSEKKELLNQHDLYEQFLRLSLLKAIDDNWVEQVDYLQQLSMAIGGQSA
                     SQKNPIVEYYQEAYAGFEAMKEQIRADMVRNLLMGLVEVTPKGEIVTHFP"
     gene            complement(5785..6225)
                     /gene="asp3"
                     /locus_tag="VW561_001298"
     CDS             complement(5785..6225)
                     /gene="asp3"
                     /locus_tag="VW561_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HEU9943554.1"
                     /translation="MIITQRQSIYWGEVGGTYMYGTTVSYYLDKSVRLYNPLLPSGEI
                     LKTWFSSVNYQAARTQPQLPLLKRKQEYQLSLVFDCQPENGVYTKITFFDRYGDILEK
                     KVEKAKDFIFTYPEDSYTYQVSLLSAGFESLTFYHFSIKEIRSV"
     gene            complement(6222..7757)
                     /gene="asp2"
                     /locus_tag="VW561_001299"
     CDS             complement(6222..7757)
                     /gene="asp2"
                     /locus_tag="VW561_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HEU9943555.1"
                     /translation="MSKELNILQIGLANWENHYDIPENMSWYYFYPNSSKALREIIEK
                     EDINRFHAVLIEDGQYSRDLFSYVKYFEPYTLFYNQNLQINDREVVDFLKKRCAQAID
                     FLSPQQLINDLSKSLFGGGYGDKLFPPTIQVNPNFTGAISYQGLDYVSLEGEFGQDFA
                     QLAYWAYNIMVQKTLPIELWLEYEKEGNCDFRLVIRKMWSGSVDDFFEEVIVSEKDLE
                     QALFMDSRDGDYFLSISVEARGRGTIKLGNLHQRWSRKQFGKFVLGGNILHDSKRDEI
                     NYFFHPGDFKPPLTVYFAGYRPAEGFEGYFMMKTLGCPFILFSDPRLEGGAFYLGTDE
                     LEGKVKDTITHYLDYLGFDHKDLILSGLSMGTFPALYYGASFEPHAIIVGKPLANLGT
                     IASRGRLDAPGVSNLAFDCLIHHTGGTSSQDMTELDQRFWKIFKQANFSKTTFGLSYM
                     KDEEMDPQAYEQLVSYLCNTGAKILSKGTAGRHNDDTDTNISWFLHFYRMVLETGFGR
                     EKR"
     gene            complement(7741..9321)
                     /gene="asp1"
                     /locus_tag="VW561_001300"
     CDS             complement(7741..9321)
                     /gene="asp1"
                     /locus_tag="VW561_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HEU9943556.1"
                     /translation="MYYFIPAWYGSERTWHADITPWYFSHFRLEFDDTFHQIRLFQEQ
                     DIDSRLLVLAYQPHLRYFLYRHGVLETDTYSVFDVMQDFHNLHTQVLSIRDIEWDDDC
                     EFIYSPFTIIVQKNGKKFAKVEHGVEGFISDIQYFEPNGQIHMHHIMDDRGFVSSIIF
                     FEDGQAAYQEYLNPKGVWQFRERLKEGGQVEVNPILGYRFKMLTYQNMGDLVAEFFEN
                     YLQTYVKDQDIFMLPSHSHHDQLVLDRLPSTNPKLLSLFIGRNSQDTFRDLDVTFEKS
                     DLILVDREDSLRLLQELYPERMHQCYHLSSFDTRLRLGRSQTKKESIIYFQLDFEQGI
                     DNQALLQVLSFVAENKDTEVIFGAFAASQEQMNEVEGIVESFIQENIQSENLGKAIDY
                     GDAENPLEENQHQDLRLQFVNLNDELDLIKTLEFVRLIVDLNRHPHLYTQIAGISAGI
                     PQINLVETVYVEHLKNGYLLADVTEFSKAAHYYTDRLKEWNESLIYSIDKIKEHTGQQ
                     FLGKLEKWIEEVKNVKGT"
     gene            complement(9331..10548)
                     /gene="secY2"
                     /locus_tag="VW561_001301"
     CDS             complement(9331..10548)
                     /gene="secY2"
                     /locus_tag="VW561_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HEU9943557.1"
                     /translation="MTKIYSSITVKKGLFTLFLLFIYVLGSRITLPFVDLNSKDFLGG
                     STAYLAFSAALTGGNLRSLSIFSVGLSPWMSAMILWQMFSYSKKLGLSSTAIEIQDRR
                     RMYLTLLIAVIQSLAVSLSLPVQSSYSVILVVLMNTLLLIAGTIFLVWLSDLNASMGI
                     GGSIVILLSSIVLNIPQDVIETFKLVHIPTGIVLLLVFMTIIFSYLLALMYRARYLVP
                     VNKIGLHNRFKRYSYLEIMLNPAGGMPYMYVMSFLSVPAYLFTLLGFIFPNHSGLAAL
                     SKEFMVGKPLWVYVYISVLFLFSIIFAFVTMNGEEIADRMKKSGEYIYGIYPGADTSR
                     FINRLVLRFSVIGGLFNVVMAGGPMLFVLFDEKLLRLAMIPGLFMMFGGMIFTIRDEV
                     KALRLNDTYRPLI"
     gene            complement(10605..11282)
                     /locus_tag="VW561_001302"
     CDS             complement(10605..11282)
                     /locus_tag="VW561_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9943558.1"
                     /translation="MGISVAKGEYITIVDSDDIVKENMIETLYQQVQEKDADVVIGNY
                     YNYDESDGNFYFYVTGKDFCVEELAIQEIMNRQAGDWKFNSSAFILPTFKLIKKELFN
                     EVHFSNGRRFDDEATMHRFYLLASKIVFINDNLYLYRRRSGSIMRTEFDLSWARDIVE
                     VFSKKISDCVLAGLDVSVLRIRFVNLLKDYKQTLEYHQLTDTEEYKDICFRLKLFFDA
                     EQRNGKS"
     gene            complement(11267..11509)
                     /locus_tag="VW561_001303"
     CDS             complement(11267..11509)
                     /locus_tag="VW561_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9943559.1"
                     /translation="MSELISVVVPIYNTGKYLVECVEHILKQTYQNIEIILVDDGSTD
                     NSGEICDAFMMQDNRVRVLHQENKGGQHKLKIWGLV"
     gene            complement(11509..12705)
                     /locus_tag="VW561_001304"
     CDS             complement(11509..12705)
                     /locus_tag="VW561_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEU9943560.1"
                     /translation="MRKSIVLAADNAYLIPLETTIKSVLYHNRDVDFYILNSDIAPEW
                     FKLLGRKMEVVNSTIRSVHIDKELFESYKTGPHINYASYFRFFATEVVESDRVLYLDS
                     DIIVTGELATLFEIDLKGYSIGAVDDVYAYEGRKSGFNTGMLLMDVAKWKEHSIVNSL
                     LELAAEQNQVVHLGDQSILNIYFEDNWLALDKTYNYMVGVDIYHLAQECERLDDNPPT
                     IVHYASHDKPWNTYSISRLRELWWVYRDLDWSEIAFQRSDLNYFERSNQSKKQVMLVT
                     WSADIKHLEYLVQRLPDWHFHLAAPCDCSEELTSLSQYTNVTVYQNVLHSRIDWLLDD
                     SIVYLDINTGGEVFNVVTRAQESGKKIFAFDITRKSMDDGLYDGIFSVGRPDDLVDRM
                     KNIEIE"
     gene            complement(12714..13934)
                     /locus_tag="VW561_001305"
     CDS             complement(12714..13934)
                     /locus_tag="VW561_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HEU9943561.1"
                     /translation="MLQDKKSVVFAGDYAYIRQIETAMKSLCRHNSHLKIYLLNQDIP
                     QEWFSQIRIYLQEMGGDLIDCKLIGSQFQMNWSNKLPHINHMTFARYFIPDFVTEDKV
                     LYLDSDLIVTGDLTDLFELDLGENYLAAARSCFGAGVGFNAGVLLINNKKWGSETIRQ
                     KLIDLTEKEHENVEEGDQSILNMLFKDQYSSLEDQYNFQIGYDYGAAAFKHQFIFDIP
                     LEPLPLILHYISQDKPWNQFSVGRLREVWWEYSLMDWSVILNEWFSKSVKYPSKSQIF
                     KLQCVNLTNSWCVEKIDYLAEQLPEVHFHIVAYTNMANELLALTRFPNVTVYPNSLPM
                     LLEQIVIASDLYLDLNHDRKLEDAYEFVLKYKKPMIAFDNTCSENLSEISYEGIYPSS
                     IPKKMVAAIRSYMR"
     gene            complement(13959..14741)
                     /locus_tag="VW561_001306"
     CDS             complement(13959..14741)
                     /locus_tag="VW561_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9943562.1"
                     /translation="MKDLISVIVPVYNVEPFISSCLDSLSKQIYQNFEVLLVNDGSTD
                     NSGAICREYADRDSRFHYFEKENAGVADARNFGIERSKGDYITFVDSDGLFYIHVFDS
                     DYYVKNYNSKLLMEELPLLERYDMSFLTSWGILFKRELFQEVQFPFGRVCEDIGTNYK
                     LFMQVEKVTYINKVLYWYRVGKEGLSNSYSLKMMRDDCDFRLERIAVLALKGYDVSKY
                     LDQMKFYLKYRHDIAIQRELKENVETRRLEMLDYLLNGNKYN"
     gene            complement(14738..17191)
                     /locus_tag="VW561_001307"
     CDS             complement(14738..17191)
                     /locus_tag="VW561_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HEU9943563.1"
                     /translation="MNKTIVLAGDRNYTRQLETTIKSILYHNRDVKIYILNQDIMPDW
                     FRKPRKIARMLGSEIIDVKLPEQTVFQDWEKQDHISSITYARYFIADYIQEDKVLYLD
                     SDLIVNTSLEKLFSICLEEKSLAAVKDTDGITFNTGVLLINNKKWRQEKLKERLIEQS
                     IVTMKEVEEGRFEHFNGDQTIFNQVLQDDWLELGRAYNLQVGHDIVALYNNWQEHLAF
                     NDKPVVIHFTTYRKPWTTLTANRYRDLWWEFHDLEWSQILQHHMGEFELISPLDKEFS
                     CLTLTNSQNLEGIEELVTALPEVVFHIAAWTDMGDKLKKLAVYNNVRLHPQIVPPVLD
                     KLKKSTNLYLDINHGSADENFLKSLQEQEKTLLAFQSTQHGELGQIVFENGKVSFMID
                     TIKDFKKNGHLTCFRQLPSLTCLTFTASQDIEQLDYLAGQLPNVVFQVAAWTAMGPKL
                     YDLSNRYPNIQLYPAISRDKLDELKEKMDAYLDINLLTSTSDIVAEMAHLSKPILAFY
                     KSQNGNNGQRLYSSEHPERMLADLQKLITKDMLEKPLDIIQVKGIDETLDYIIEHNSS
                     LVRFGDGEVNLMWGLPIPYQNHDLELANQLKHIVGLESDEKLVVCLPDAFDDRFVFTW
                     WATPFWKEHVNVYMDFYKELCKGSWYGSTFISRPYIDYEDKSKAKGQFEKLKSIWENR
                     DILIVEGITSRSGVGNDLFDKVKSVKRIICPSHNAYSVVDNIQEEIMKHAEGRLILCM
                     LGPTAKVLSYNLCQMGYQVLDVGHIDSEYEWMKMGAKTKVKFSHKHTAEHNFDQDIEF
                     IDDETYNSQILVDLSKREV"
     gene            complement(17201..18211)
                     /locus_tag="VW561_001308"
     CDS             complement(17201..18211)
                     /locus_tag="VW561_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU9943564.1"
                     /translation="MKLHLTNLYGMAGDSTVILAQNAVQKIASQLGFREVGIYFYNIA
                     SDSPSEMNKRLDGIMASISIGDILVFQSPTWNGFEFDRLLFDKLKDMQVKIICFIHDV
                     VPLMFDSNYYLMKDYMYMYNLSDVLIVPSERMKTRLMEEGLTTKKILVQGMWDHPHDL
                     SLYTPAFKKELFFAGSLERFPDLQNWSQDTPLRVFSNKGEASSSARNLSIEGWKKDEE
                     LLLELSKGGFGLVWGTHQNEGESNQYYTLNISHKVSTYLTAGIPVIVPSSLSTAKFIV
                     DQGLGFMADSLEEVHEIVDKMNLQEYQEMTNRIKTFSYLLKEGYFTKKLLVDAIYHLG
                     ID"
     gene            complement(18240..18830)
                     /locus_tag="VW561_001309"
     CDS             complement(18240..18830)
                     /locus_tag="VW561_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEU9943565.1"
                     /translation="MEHDALDRLYGALKKENADISIGRYNSYDETRYVYMTYVTDPDD
                     SLEVIEGKAIMDREGVEEVRNGNWTVAVLKLFKRELLQDLPFPIGKIAEDTYWTWKVL
                     LRASRIVYLNRCVYWYRVGLSDTLSNTWSEKRMYDEIGAREEKIAILASSDYDLTNHI
                     LIYKNRLQRVIAKLEEQNMQFTEIYRRMMEKLSLLP"
     gene            complement(18835..19116)
                     /locus_tag="VW561_001310"
     CDS             complement(18835..19116)
                     /locus_tag="VW561_001310"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9943566.1"
                     /translation="MENELISIVVPIYNVENYLRMCLDSIQNQTYQNFECLLINDGSP
                     DHSSKICEEFVEKDSRFKYFEKANGGLSSARNLGIECSGGGVHYFCRLG"
     gene            complement(19156..20034)
                     /locus_tag="VW561_001311"
     CDS             complement(19156..20034)
                     /locus_tag="VW561_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HEU9943567.1"
                     /translation="MKKNHLVGDALILTVSDQIEELDYLLESLPNICFHIAAPVQFSE
                     KIRSLETNYNVRLRTITNEEQLNFLVDTCDFLLDINHFQEVDAIVSKFVQAGKSVFAF
                     DNTVHGNQGQEVFLSSTPDKLVSRVRDYLNEVRVGTNHQEKIIQDGTWNVFKIDDKAH
                     FIVGANVICRNFENFHVSSGKLILHDGVFINNSCSFNCMERIEIGAGTMMGEGVRFYD
                     HDHIYTAEKIEKWQWTTAPIRVGRDCWIGSNVTILKGVTIGDNTIIGAGCLIRNDIPS
                     NSVVYNNGNLVVKRRD"
     gene            complement(20031..22115)
                     /locus_tag="VW561_001312"
                     /pseudo
     CDS             complement(20031..22115)
                     /locus_tag="VW561_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000341103.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(22176..>22703)
                     /locus_tag="VW561_001313"
     CDS             complement(22176..>22703)
                     /locus_tag="VW561_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9943568.1"
                     /translation="SASASASTSASASASTSASESASTSASDSASTSASASASTSASA
                     SASTSASASASTSASESASASTSASASTSASASASTSASASASTSASASASTSASASA
                     STSASASASTSVSNSANHSNSQVGNTSGSTGKSQKELPNTGTESSTGSVLLGVLAAVT
                     GIGLVAKRRKRDEEE"
BASE COUNT         7251 a         4868 c         3510 g         7074 t
ORIGIN      
        1 gtgttttgag ctgacttcgt cagttctatc tacaacctca aaacggtgtt ttgagcagcc
       61 tgcggctagc ttcctagttt gctctttgat tttcattgag tataaaaaag aaaacttcag
      121 accaatccac aaaaggtgaa tatctgaagt tttcttttat ttataagtaa tcaccataaa
      181 ggttaatagt agaacaaata aagccaaact caccaataaa gtgagcacct tgaccaaggt
      241 gttgctctgc cagtagcttg gtttaccaat attactagtg gcatccatgg aaaatagttg
      301 attttgacgg ggttgtatag ttaccaatac aatcaaagca agagatagga taatagctaa
      361 aacaattagt atttcaatca tagatctacc tcaaaattcc cagcaaagta aagagcaaac
      421 caatcagaat cattaagccc agtaaaagtg aaaaagtctt actaatcttt tcccctcggg
      481 tttctttttc cttcttgatg ggtttttgtt tcaactcttc catgcggcct tcaacgtctt
      541 tgtaaaataa atcttcctct gacatgttag cctcctaaaa cagtttgcat ggtttcctga
      601 tatctcacca agtcaacata atttgcatgt tggccttgtt tgccaagtgc ctgacgcatt
      661 tgatcaacat ctgaaagggc caatttaatg gtctcaacca aagcagcaac ttcactactt
      721 tcaaatagat ggtctggagt gatataaagt ctattgtgca ccgtctgatt aaagccaaga
      781 atcaagagat tgtgctcaaa ggcctgacgc actgcctgta gcaactcatt actgtggttt
      841 atatccaagt aaatatccga cagttgatac agctcctgaa tcttctgtgg actagcgttc
      901 tgataaagga ccacattagg ataacgaagc atatctaaga gcttagaaga catctctgtc
      961 accgctgcaa tacggaaagt gacatcaggc aaggctcctg cgattgcttc tacttgctca
     1021 atctgatctg aattggtcaa gattaaggca tctcgtctta ggaaattatc acgtttgaac
     1081 tggtaatgat aacccaagtg cacaaactga tcatggtatt tctcgtcagt taactctaaa
     1141 gcgcgctcat aagtcgtctt atttggaatg atgatcttct tagtacgcac attatcactt
     1201 tccaaaatca actgcatatt acctggaatg gcatcataaa gaggttcctg ccataccaag
     1261 acatccgagc cagatttatc tggataatga aaggaaacca agaaaggagt cgctagagta
     1321 ttaaagataa gctgactggt gtctatttcc aaatcttgca aaaagaaggt gataaattca
     1381 actttatttg caaagtaacg catggactga ccaggcaaag tcaataagat atcacccgtc
     1441 acatggtttt ccagtaaaac ttgttgacca ttgacatctt ggtaaactgt cataatcggc
     1501 tcgctatctg cgctataagt cgttgtagca aaacaagctc cgaagcgatt gtagtggtca
     1561 acctgacgta ctcgaccttc taggtctttc cagtctacct gtttaaccaa gcgagcctgc
     1621 attccatcag cataatgaat gacagccctc tcctgcgtca catcttcaat acaagcagac
     1681 tgattatctc ctaaaatttc ccaaaaatct gaaacgggaa cttgattaaa atagagaggt
     1741 tttccatcct cgtaacctag ataataggta aaaggagaaa gcagaccatc aggcagaaaa
     1801 ccatctgcat cgatgaccac tccaagttga gaaagaccag tagcgcctag actttcatgt
     1861 aaatctcgac tttcttgact gtaactatca taaagttcaa tcatggagca cctcctctac
     1921 tgttttcttc cacttttcta aaatttcttt ggtcaagaag ccttctgcaa tttggtaaga
     1981 ataggcacgc atagcttcca aacgattttc ttgatacaat tgacaaatct tagcggcata
     2041 agcttgcttg atttggtctt ctacatggtc agatgaactt ggaatcaaat aaccattttg
     2101 cccatcctct ataaaggtct gattaccata aggcacatca aaaccaatta gaggtagacc
     2161 tgaaccaata gcttccatca aggtcagacc aaatccttcg ctggtagaag ccgttaagta
     2221 gacctcatac tggctataaa tctgcgaaag ttccgcatgc cccttgagtt ggatatagtc
     2281 ctctgcctga tgatttgcaa taatgtctct aagcagagaa tcttctccac cactaccata
     2341 gatatcaaag gttagttccg gtaactcctt atgagcttca atcacagctt tcacaagcca
     2401 atcaatgtgc ttttctttgg caagacgtga agctgtaatc aatgaaaatg gtttgcgccc
     2461 ttgacttgaa tctgtcaagg aatcaatact gcctacagga atggtaacaa tctttggctg
     2521 atgctgagta tatttggcaa attgctcttg tagaacttca ttttgtctat cagtagacac
     2581 gataaagaag tcaaccttat ctgcattggt aaactgatag tcataatagt tattccaaag
     2641 gatgtagtcc tcatttgtag cattttcact ataatgctcc gcatgaacaa ctaccgctaa
     2701 atgtgctgtc tgtgcttcct caaacacaac ctgaccaatg cctgtctccc tatcgagaat
     2761 gaccaaatca gacttattca aattcaaaga tttcataaag gcacgcacaa aagcttgctt
     2821 tccatagaaa atcttatcct tgaaatgata aacttcttcc ttcccttgat tcatcaagat
     2881 atcatagact ggagtcccgt cttcattata aaaagttcgt tggtataaga ctgcaacatt
     2941 gtccttggga gcaaaatact cgctacaata acgcgtataa gaaaagtaat ccttccgaat
     3001 caggtttccc ttaaaaacat actcggcatg ttgaaccaag tccttgttct catcaaccaa
     3061 ataacaggtt acaaacttat cttggtcaaa aaagaagaca cgtaaaacct tgccattttt
     3121 ttctctgtga ctttcttcac caccaaagta agccaagaca tcatccactg tcacgctagt
     3181 aggcgcaatt ttgatatctg tgaaatgatt ataaagccag ataacctgat tatcatcaaa
     3241 accaatattg gctgttaagt gctgaatatt atcggctaaa atcatatctg taaagataaa
     3301 cttagaggac agatttaatt tccgaaaaac accagcacga taggcttgag cgtattcaac
     3361 accgctacta gcccaaccaa ttcctaaatt tatattgtaa attgtcatat tttctcctct
     3421 tatggaaaat gagtcacgat ttcacctttt ggagtgacct caaccagccc catcaggaga
     3481 ttacgcacca tatccgcacg gatttgttct ttcatagctt caaagcccgc gtaggcttct
     3541 tgatagtact ctacgattgg atttttctga ctagcagatt gaccaccgat agccatggat
     3601 agctgttgta gatagtctac ctgctctacc cagttgtcat caatggcttt aagcagtgaa
     3661 agtcgtaaaa actgttcata taagtcatgt tgattaagta attctttctt ttcagaaagt
     3721 tctttatcaa tcacctgctt cataaagcta cgaactgcag ttttgtcagt tacatctata
     3781 taatctggaa cctctttaac atgaaaacta atattggtca caataaagtg aaacagtaat
     3841 tcacgactag catagtgatc agccgctacc tcttctgtat atctctcaat gatatccaca
     3901 acaacatcct ctaagtcacg agaaccatct attagacgat tacgctcctt gtaaatcata
     3961 tcccgttgaa tattcatact ttcagcatat tctagagtct gacgacgtgc cgaacggcca
     4021 gcactatcac tggcatgctg agccttttcg actagtttcc ggtatttacg acctttcaat
     4081 acttccggtt gagtcatatc ttgaacctga tagtctttgt actttttatg cacccaagat
     4141 ggaccaaatt tcttgataac atcatcctct aaggatacaa aaaatttact cataccagga
     4201 tctccctgac gaccagaacg gccacgaatt tgtaggtcga tccgctgact ttccatccgc
     4261 tcagtcccaa taacaatcaa gcccccaagc tctgcgactc ctttaccaag cttgatatcc
     4321 gtaccacgtc ctgccataga ggtagccact gtcacagccc ccatctgacc tgactcggag
     4381 ataatctgag cctcacgcgc cgcattatta gcatttagga cattatgggc aatcccttca
     4441 cgaaacaaga gagacgaata gagttgagac atttcaactg agcctacaaa aacgagtaag
     4501 ggatttccct tagcatggta ttgcttgatg tactccaagg atgcatacac tttttcaggt
     4561 aaagtgatat atagattatc tggatagtca atccgttgtc tcggacgatt ggttggaatg
     4621 cgtactacag acatattgta agtttcaata aactcttttt ccgcgacctt acctgtccct
     4681 gtcataccag atatcttatt aaacatctta aaaagactct gataggtgat cgaggccata
     4741 gcccgcgtct caggagataa tttgacatgt tccttggctt caatagcctg atggagacct
     4801 ccttgaagtt tagtcatttc cattagacgc cctgtgccct tatcaaccag taccatctca
     4861 tttccacgaa tgatatagtc cttatcttta gtaaagagct tatgagctcg aatcgcataa
     4921 accaaatgac gagcaaaaga cgcatgctct tccttgtata aattatcaat ccctaggaaa
     4981 ttctcagcag acttggcccc cttagtagtg agccaaacct cctctttctc ctctttaaag
     5041 atataatcct ctccttcgac caaggttgtt acaagtgtat taatgatcgc atagtaatta
     5101 gactgaacac gaggagaacc cgcaataatc agaggagttt gtgcactatc aagcaagata
     5161 tcatcaattt catcaataat cacatagtta aacggtcgta aaaacttacc ttcttcattc
     5221 gaggctaggt tatcatttag ataatcaaaa cctaaattac tattggttgt gtagatgata
     5281 tccgaagcat agataagctt cttttcttca gctttcatct ccttctttgg atcttccgta
     5341 aatggtacac caatggtcaa tcctagaaaa cgataaactt gacccatttc ctcggcatca
     5401 cgctttgata aatactcatt aggagtcaca accatcactc cttctcctga aaaagcgttc
     5461 aaatagacag gcatggtagc tgtcaaggtc ttaccttccc ccgtattcat ctcagcaaca
     5521 tttccatagt gcatgacaat agctcccatg acttgaacat cataaggaaa catccctaaa
     5581 atccgcttat ctgcttcacg caccacagca aaagcttcaa ccaaaatatc gtctagactt
     5641 tctccctcgg aaagacgctg acgaaactct actgttttag ctactaattc ttgatccgaa
     5701 agagaggaca tcttgccttt cagggcatta atctgcttta aaatcttttt cactttccta
     5761 agctggaaat cttgacctaa acgtctaaac acttctgatc tccttgatag aaaaatgata
     5821 aaaagttaag gactcaaatc cagcacttaa aagagaaact tgataagtat aactatcttc
     5881 tggataagta aatatgaaat ctttcgcttt ttctaccttt ttttctaaaa tatcaccata
     5941 gcggtcaaaa aaagttatct tggtataaac tccattttca ggctgacaat caaaaaccag
     6001 tgatagttga tactcctgct ttctttttaa taagggaagc tgaggttggg ttcgtgcagc
     6061 ctggtaattg acactagaaa accaagtctt tagaatctca ccagatggca atagaggatt
     6121 atacagacga acacttttgt caaggtaata agatacagtt gttccataca tataagtccc
     6181 accaacttct ccccaataaa tagactgtct ctgtgtaata atcatctttt ctcccttcca
     6241 aaaccagtct ctaagaccat tctataaaag tgcaaaaacc aagaaatatt ggtatctgta
     6301 tcatcattgt gtcgtccagc agttccttta gataaaatct tcgcacctgt attacacaga
     6361 taagacacta attgttcata ggcttgtgga tccatttctt catctttcat ataggataaa
     6421 ccaaaggttg tctttgaaaa atttgcttgt ttaaaaattt tccaaaaacg ctgatccaac
     6481 tccgtcatat cttgagaact tgtcccacct gtatgatgaa ttaaacaatc aaaagctaag
     6541 ttagagacac ccggtgcgtc caaacgtcca cgactagcta tagttcctaa attagccaag
     6601 ggcttaccaa ctatgatggc atggggttca aaagaagcac cataatagag agccggaaac
     6661 gttcccatag aaagacctga taaaatcaaa tccttatgat caaagcctaa ataatcaaga
     6721 tagtgagtga tcgtatcctt tacttttccc tctagctcat ccgttccgag ataaaaagct
     6781 cccccctcta aacgtggatc agaaaataaa atgaagggac atccaagagt tttcatcata
     6841 aagtaaccct cgaatccttc tgcaggtcga taacctgcaa aatagacagt caaaggcggt
     6901 ttaaaatcac ctggatggaa gaaatagttt atttcatcac gcttggaatc atgtaggata
     6961 tttccaccaa gtacaaactt accaaattgt tttcgactcc atcgttggtg aagattacct
     7021 agtttgatag ttccacgacc tcttgcttca acagatatcg agagaaagta gtctccgtct
     7081 cgactatcca taaaaagtgc ttgctccaag tctttttcag atactattac ttcttcaaag
     7141 aaatcatcaa cagacccact ccacatttta cggatgacca aacgaaagtc acaattgcct
     7201 tccttctcat attcaagcca caactcaata gggagtgttt tttgcaccat aatattataa
     7261 gcccaatagg caagctgggc aaaatcttgc ccaaactcac cttccaaact gacataatcc
     7321 aatccttgat aagaaatagc tcctgtaaaa tttggattga cttgtatcgt cggaggaaag
     7381 agtttatcac catacccacc gccaaaaaga gatttactta aatcattgat tagttgttgg
     7441 ggacttaaaa aatcaattgc ttgtgcacat cgttttttta gaaaatccac aacctctcta
     7501 tcatttattt gtagattctg gttatagaac aaagtataag gttcaaaata ttttacatag
     7561 gaaaataagt ctctggaata ctgaccatct tctattaaaa ctgcatgaaa acggttaata
     7621 tcctcttttt caattatttc acgaagggct tttgatgagt ttgggtaaaa ataataccaa
     7681 ctcatatttt caggtatgtc atagtgattt tcccaattgg caagtcctat ctgtaaaata
     7741 ttaagttcct ttgacatttt taacctcctc tatccatttc tctaattttc caagaaattg
     7801 ttgtcctgtg tgctccttaa tcttatcaat tgaatatatc aaggactcat tccactcctt
     7861 caacctatct gtgtaataat gtgcagcctt agaaaattct gtaacatctg ctaacaaata
     7921 accatttttt aaatgttcaa catagacggt ttcaactagg ttgatttgag gaattcctgc
     7981 actaatccca gcaatctgtg tgtagagatg aggatgtcta tttaaatcca caatcaaacg
     8041 gacaaattct agtgttttta tcaaatctaa ctcatcattc aagttaacaa actgtaagcg
     8101 taagtcctgg tgttgatttt cttccagagg attttctgca tcaccataat caatcgcctt
     8161 tcccagattt tcggattgaa tgttttcttg gatgaaagac tcaacaatcc cttcaacctc
     8221 attcatttgc tcctgactag cagcaaaggc tccaaaaatc acctcagtat ccttattttc
     8281 agcgacaaag gacaagactt gaagcagagc ttgattatca atcccctgct caaaatccag
     8341 ttgaaaataa atgatggatt ctttcttagt ttggcttcgt cccaatcgta atcgggtgtc
     8401 aaaagatgat aaatgataac attgatgcat tcgttcagga tacaactcct gcaacaatcg
     8461 taaactatcc tctctatcca ccaaaatcaa atccgatttt tcaaaagtta catctaaatc
     8521 cctaaaggta tcttgagaat tacgtccaat gaacagactc aacagtttag gattagtact
     8581 aggtaaacga tctagtacca actggtcatg atgagaatga gaaggaagca taaaaatatc
     8641 ctgatccttc acatacgttt gcagataatt ctcaaaaaat tctgccacca gatctcccat
     8701 attttgatag gtaagcattt taaagcgata acccaaaatt ggattgactt ctacctgtcc
     8761 tccttctttt aaacgctctc taaattgcca aactccctta ggattcaaat attcttgata
     8821 ggctgcttgc ccatcttcaa aaaagataat gctcgataca aaccctcgat catccatgat
     8881 atggtgcata tgtatttgac cattcggttc aaaatactgt atatcactga tgaagccttc
     8941 aactccatgt tcaaccttag caaatttctt cccatttttt tgaacgataa tcgtaaaggg
     9001 actataaata aattcacagt catcatccca ctcaatatct ctaatgctta aaacttgggt
     9061 atggagatta tgaaaatctt gcataacatc aaaaacggaa taagtatccg tttctaacac
     9121 accatgtcta tataaaaaat aacgtagatg aggctggtaa gctaatacta atagacgaga
     9181 atctatatct tgctcttgaa agagccggat ctggtgaaag gtatcatcaa actctagacg
     9241 aaaatgagaa aaataccatg gagtgatatc tgcatgccat gttctttctg acccatacca
     9301 agctggaata aaataataca taaaagtctc ctaaatcaag ggtctatagg tatcatttag
     9361 ccttaaagcc ttgacctcgt ctctaatcgt aaaaatcatg cccccgaaca tcataaataa
     9421 gcctggaatc attgccaatc gtaataactt ttcatcaaac aaaacaaaaa gcatgggacc
     9481 acctgccata accacattaa agagaccacc tatgactgag aaacgaagga ccaatcgatt
     9541 aataaatcga ctagtatccg cacctggata aataccataa atgtattctc cagatttttt
     9601 catacggtct gcaatctctt ctccattcat cgtaacaaaa gcaaaaatga tactaaataa
     9661 aaataagacc gaaatataaa catagaccca caaaggctta ccaaccataa attcctttga
     9721 taaagccgct agccctgaat gattagggaa aataaatccc aacaaggtga acaaataagc
     9781 tggtacacta agaaaactca tcacatacat ataaggcatc ccacctgcag gattcaacat
     9841 gatttcgaga taagaatagc gtttaaatcg attgtgtaag ccaattttat taacaggaac
     9901 caaatagcga gctcgataca taagggcaag caagtaagaa aatataatgg tcataaaaac
     9961 aagcaagaga acaatccctg tagggatatg aaccagttta aaagtttcaa taacatcctg
    10021 agggatattt aaaacaatgc tggataggag gattacaata gaacccccaa ttcccatgct
    10081 agcatttaaa tctgacaacc aaaccagaaa aattgttcct gctatcagca acaaggtatt
    10141 cattagaaca accaaaatta ccgagtagga ggattgtact ggcaagctca agctaactgc
    10201 caaggattga atcacagcaa ttagcaaggt cagatacatt cttcgacgat cttgaatttc
    10261 aatcgctgta gaacttagcc ccaatttttt agaatatgaa aacatctgcc acaaaatcat
    10321 agctgacatc caaggggata atccaacaga aaaaattgac aaacttctta gatttccacc
    10381 tgtaagggct gctgaaaaag ctaggtaggc tgttgaacca cctaagaaat ctttggaatt
    10441 caaatcaaca aaagggagag taatacgact tcccaataca tagataaaca gtagaaataa
    10501 ggtaaataat ccttttttta ctgttataga cgaataaatc ttagtcataa aacacgcccc
    10561 tcaccttcat tgtttgtgat atggtaaata acaattcttt tatttcaact tttaccattt
    10621 ctttgttctg catcaaaaaa caactttaat ctgaaacaaa tatctttata ttcctcagta
    10681 tctgttaatt gatggtattc taaagtttgc ttataatctt ttaaaagatt gacaaatcga
    10741 atacgcagaa cggagacatc caaaccagcc aagacacaat ccgatatttt cttagaaaac
    10801 acttcaacaa tatctcttgc ccaggaaaga tcaaattccg ttctcatgat gcttcctgaa
    10861 cgtcttctat acagatagag attatcgttt ataaagacga ttttagaggc taaaagataa
    10921 aagcgatgca tagttgcttc atcatcaaag cggcgaccat ttgaaaagtg aacttcattg
    10981 aataattctt ttttaatcaa cttaaatgtc ggcaatataa aggccgagct attgaatttc
    11041 caatctcctg cttgacggtt cataatttct tgtatagcta attcttcgac gcaaaaatct
    11101 ttccctgtta cataaaaata aaaattcccg tcactttcgt cataattata gtaattccct
    11161 ataacaacat ctgcatcctt ttcttggact tgctgataaa gagtttcaat catattttct
    11221 tttacgatat catctgaatc aacaatcgtg atgtactctc ccttagctac actaatcccc
    11281 atatttttag cttgtgctgc ccccccttat tttcttgatg caatactcgc acacgattat
    11341 cttgcatcat aaaagcatca caaatttccc cagaattatc cgtagaaccg tcatcaacta
    11401 aaataatttc tatattttga taggtttgct tcagaatatg ctcgacacac tccactaaat
    11461 attttcccgt attgtatatc ggtaccacaa cactaattaa ttcactcatt actctatctc
    11521 tatattcttc attctatcca ctaaatcatc tggtctcccc acagaaaaaa taccgtcata
    11581 gagtccatca tccatacttt tacgtgtgat atcaaaagcg aagattttct tgccactttc
    11641 ttgtgccctt gtaactacat taaaaacctc tccacctgta ttaatatcta aataaactat
    11701 agaatcgtcc aatagccaat caattctact atgtaataca ttttgatata ctgttacatt
    11761 cgtatactgt gatagagagg tcagctcctc agaacaatca cacggtgcag ccaaatgaaa
    11821 atgccaatca ggtaaccgtt gtactaaata ctctaaatgt tttatatctg cactccatgt
    11881 cacaagcatc acttgttttt tagactggtt gcttctttca aaataattta aatcggaacg
    11941 ttgaaaagca atctctgacc aatccaaatc tctataaacc caccataatt cacgtagtct
    12001 agatatacta tatgtattcc aaggtttatc atgactagca tagtgaacaa ttgtaggtgg
    12061 attgtcatct agacgttcac attcttgagc aaggtgataa atatcaacac ccaccatata
    12121 attatatgtt ttatctaagg ctagccaatt atcctcaaaa taaatattta aaatactctg
    12181 atccccaaga tgaacaactt gattctgctc ggccgctaat tccaataaac tattgacaat
    12241 agaatgttct ttccactttg caacatccat tagtaacata ccagtattaa atccagattt
    12301 tcgtccttca taggcataga catcatcaac agcaccaatt gaatatcctt tgagatctat
    12361 ctcaaacaaa gtagctagtt ccccagttac aatgatatcg gaatccagat acaataccct
    12421 atcagattca accacttctg tcgcaaaaaa tctaaagtaa gaagcataat ttatatgagg
    12481 tcctgtttta tagctttcaa aaagttcttt atcaatgtgt acactgcgaa ttgtagaatt
    12541 cacaacttcc atttttctcc ccaataattt aaaccattca ggagctatat cactgttgag
    12601 aatataaaaa tcaacatctc tattgtgata caatacagac tttatagtcg tctctaaagg
    12661 aataagatag gcattatctg ccgctaatac tattgatttt ctcatactgt tctctacctc
    12721 atgtaagatc tgattgctgc aaccattttt ttcggaatgg agcttggata gataccttca
    12781 tatgaaatct cagaaagatt ttcagagcaa gtattgtcga aagctatcat tggttttttg
    12841 tacttaagca caaactcata tgcatcttct aattttcgat catgattcaa atccaaatac
    12901 aaatctgaag ctattactat ttgttccaat aacattggta aggaatttgg atatacggta
    12961 acattaggaa aacgcgttaa agctagtagt tcatttgcca tatttgtata agcaacaata
    13021 tgaaaatgaa cttctggcaa ttgctccgcc aaataatcga ttttctcgac acaccaagaa
    13081 ttcgttaaat taacacattg taacttaaat atttgtgatt tactaggata cttcacactc
    13141 tttgaaaacc attcatttaa aataacagac caatccatca aagagtattc ccaccaaact
    13201 tctcttagac gtccaacaga aaattgattc caaggcttat cctgagaaat atagtgtaaa
    13261 attagtggca gtggttcgag cggaatatca aaaatgaatt gatgtttaaa ggctgccgcc
    13321 ccataatcat atcctatttg aaaattatat tgatcttcaa gggaactata ttgatcttta
    13381 aacaacatat tcaaaattga ctggtctcct tcttccacat tctcatgttc tttttctgtt
    13441 aagtcaatca atttttgtcg aatagtttca gatccccatt ttttgttgtt aatcaagaga
    13501 acaccagcat tgaagccgac tcctgctcca aagcaagaac gagctgctgc caaataattt
    13561 tcacctaagt ctaattcaaa caaatcggtc aaatcaccag tcacaatcaa atcactatct
    13621 agatagagaa ctttatcttc tgttacaaaa tctggaataa aatagcgtgc aaatgtcata
    13681 tgattgatat gaggtaattt attagaccaa ttcatttgaa actgtgagcc aattaacttg
    13741 cagtcaatca agtcgccccc catctcttgt aaatatattc ttatttgact aaaccattcc
    13801 tgaggaatgt cctgatttag cagataaatt ttcaaatgac tattgtgtct acagagtgac
    13861 ttcatcgccg tttcgatttg tcgaatataa gcgtaatcac ctgcaaatac tacagatttc
    13921 ttatcttgta acattttatc gtctctctat cttaattttc agttatattt attaccattc
    13981 aataaatagt ctaacatttc taaacgtctt gtttccacat tctccttaag ttctcgttga
    14041 attgcaatat catgacgata tttcaaatag aacttcatct ggtctaaata tttactgaca
    14101 tcatatcctt ttagcgctag cacggcaatt cgttctaacc taaaatcaca atcatcacgc
    14161 atcatcttca gtgaataaga attagaaagt ccttctttcc caaccctata ccaatacaaa
    14221 actttattaa tataggttac tttctcaacc tgcataaata atttataatt ggttcctata
    14281 tcctcacaca cccgaccaaa cggaaactga acttcttgaa ataattccct tttaaataga
    14341 attccccaag aagttaaaaa gctcatatca tacctctcta ataaaggaag ttcttccatt
    14401 agaagttttg aattataatt tttcacataa taatcggagt caaatacgtg aatatagaat
    14461 aagccatctg agtctacaaa agtaatataa tctcctttag atctctcgat tccaaaatta
    14521 cgtgcatccg ctaccccagc attttctttt tcgaaatagt gaaaacgact atcacgatca
    14581 gcatactctc gacatattgc tcctgaatta tctgtagagc catcatttac taataaaact
    14641 tcaaaatttt gataaatctg tttcgataaa ctgtctaagc agctagaaat aaatggttct
    14701 acgttataaa ctggtactat aacacttatt aaatctttca tacttctctt tttgataaat
    14761 caactaaaat ctgactgtta taggtttcat catcaataaa ttcaatatct tggtcgaaat
    14821 tatgttctgc agtatgttta tgagaaaatt taaccttagt tttagctccc attttcatcc
    14881 attcatactc tgagtcaata tggcctacat ccaaaacttg atagcccatc tggcatagat
    14941 tataactcag aacttttgct gtaggaccca gcatacataa aatcagtctt ccttcagcat
    15001 gtttcataat ttcctcttga atgttatcga caacggaata agcattatga gatggacaaa
    15061 taatccgttt cacagatttg accttatcaa ataaatcatt tccaacccca gagcgagatg
    15121 taatcccctc tacaatcaga atatccctat tctcccaaat actctttagc ttttcaaatt
    15181 gtccttttgc cttactctta tcttcataat caatgtatgg acgcgagata aaggtagagc
    15241 cataccatga acccttacac aactctttat aaaaatccat ataaacattc acatgttctt
    15301 tccagaatgg tgtagcccac caagtaaaga caaaacgatc gtcaaaagca tcaggtaaac
    15361 aaaccactaa tttctcgtca ctctcaagac ccacaatatg ttttaattgg ttagctaact
    15421 ctagatcatg attttgataa ggtataggca aaccccacat caaattgact tctccgtctc
    15481 caaaacgaac taaagaagag ttgtgttcaa taatataatc caaggtttca tctatccctt
    15541 tcacctggat tatatcaagc ggtttttcta gcatatcctt agttatcaat ttttgcaaat
    15601 cagccaacat tcgttcagga tgttcacttg aatacaacct ttggccatta ttcccatttt
    15661 gagatttata aaaggctagt ataggtttag atagatgagc catttctgca acgatatcgg
    15721 atgttgaagt cagtaggttg atatctaaat aagcatccat cttctccttc aactcgtcta
    15781 gcttatctct agaaattgcc ggatagagct gaatattagg ataacgatta gacaaatcat
    15841 ataattttgg ccccatagct gtccaagcag caacttgaaa aacaacattt ggcaactgtc
    15901 cagccaagta atccaattgt tcgatatcct gagaagccgt aaacgttaaa caagttaaac
    15961 ttggaagttg tcgaaaacag gtaagatgtc cgtttttctt aaaatcttta atcgtatcaa
    16021 tcataaagga aactttccca ttttcgaaaa cgatttgtcc taactctccg tgctgagtcg
    16081 attgaaaagc tagtagcgtt ttttcttgtt cttgcaaaga ttttaaaaag ttctcatctg
    16141 cactaccatg attgatatcc aaatatagat ttgttgactt tttcagctta tctaatacag
    16201 gaggaacaat ctgtggatgc aatctcacat tattatatac agctaatttt tttaatttat
    16261 ctcccatatc cgtccaagct gcgatatgaa ataccacctc aggtagagct gtaactagct
    16321 cttctattcc ttctaaattt tgggaattcg ttaaggttaa gcaagaaaat tccttatcta
    16381 gaggcgatat tagttcaaat tctcccatat ggtgttgtaa aatctgactc cactccaaat
    16441 catggaattc ccaccataaa tcacgataac gattagctgt caaggtggtc cagggttttc
    16501 tgtaggtcgt aaaatgaatc accactggtt tatcgttaaa agccagatgt tcctgccaat
    16561 tgttatacaa agccacaata tcatgcccta cttgtaaatt ataagctcga cctagctcta
    16621 accaatcatc ttgtaagacc tgattaaaaa tcgtttgatc accattaaaa tgctcgaaac
    16681 ggccttcttc aacttccttc attgtaacaa tgctctgttc aattagtcgt tcttttaatt
    16741 tctcttgacg ccatttttta ttgttgatta atagaacacc tgtattaaat gtaattccat
    16801 ctgtatcttt aactgctgcg agtgattttt cttctaaaca aatactaaat aatttctcca
    16861 aagaagtatt tacaatcaaa tcactgtcta aatataaaac cttatcttct tggatataat
    16921 ctgcaataaa atatctagca taggtaatgc tactaatgtg atcttgcttt tcccaatctt
    16981 gaaacacagt ttgttcaggt agtttaacat cgataatctc actacctaac atgcgagcta
    17041 tttttcgtgg tttgcgaaac caatctggca tgatatcttg attcaaaata taaatcttaa
    17101 catctcgatt gtggtataaa atagatttta tggttgtttc taactgcctg gtgtaattgc
    17161 gatcccctgc tagtacaatt gttttgttca tttcattccc ttaatcaatt cccaagtgat
    17221 agattgcatc taccaataac tttttagtga aatacccctc ttttaacaaa tagctaaagg
    17281 tcttgatacg attcgtcatt tcttgatatt cttgtagatt cattttatca actatctcat
    17341 gaacctcttc cagactatcc gccataaagc ctaagccttg atctactata aatttagcag
    17401 ttgacaagct acttggtaca atgactggga tgcccgctgt cagataggta ctcaccttat
    17461 gagatatatt caaggtatag tattggttac tttctccctc attttgatgg gttccccaga
    17521 caaggccaaa tccacccttt gataattcta gcaacaattc ctcatctttt ttccatcctt
    17581 cgatgctgag atttctagca ctagaactag cttccccttt atttgaaaat actctcaaag
    17641 gcgtatcttg agaccaattt tgtaagtctg gaaaacgctc taaacttcca gcaaaaaaaa
    17701 gttctttttt aaaagcaggg gtgtataagg ataaatcatg aggatgatcc cacatccctt
    17761 gaacaagaat cttcttagtc gtcaatcctt cttccatcag gcgtgttttc attctctctg
    17821 atggcactat caaaacatct gatagattat acatgtacat atagtctttc atgagataat
    17881 agttactatc aaacatgagg ggaacaacat catggataaa gcaaataatt ttcacctgca
    17941 tatcctttag cttgtcaaac aagagacgat caaattcaaa gccattccag gttggagact
    18001 gaaagactaa gatatcacca atagagatac tggccataat accatccaga cgcttattca
    18061 tttcagaagg actatctgaa gcaatgttgt aaaaataaat accaacctct ctaaatccca
    18121 gttgactggc tatcttttga acagcatttt gagctaagat aaccgtacta tcaccagcca
    18181 tgccgtataa atttgttaaa tgtaatttca tattatgcta tctcattttt tattactatc
    18241 tacggaagta aagacaattt ttccatcatt cttctataaa tctctgtgaa ctgcatattt
    18301 tgttcttcta attttgctat cactctttgt aatctatttt tataaatcaa aatatgattg
    18361 gtcaagtcat agtctgaact tgctaaaata gctatctttt cttccctagc cccaatttca
    18421 tcatacatac gcttttcact ccatgtattc gataaagtat cagataaacc aacacggtac
    18481 cagtaaacac aacgattcaa atagactatc ctcgaagctc ttagaagtac cttccatgtc
    18541 cagtaagtat cctctgcaat ttttcctata ggaaatggta aatcttgtag taactctctc
    18601 ttgaataact tcaagacagc tacagtccag ttcccatttc tgacttcttc gacaccttcc
    18661 ctatccataa ttgcttttcc ttctatcact tccagagaat catctggatc cgtaacataa
    18721 gtcatatata catagcgtgt ttcatcataa gaattataac gtccgatact aatatctgca
    18781 ttttcttttt tcaaagcacc atataatcgg tctaaagcat catgttccaa ccaatcatcc
    18841 gagtctacaa aagtaatgta cgcccccccc cgaacattca atacctaggt tacgagctga
    18901 tgaaagaccg ccgtttgctt tctcaaaata tttgaaacga gaatctttct ctacaaattc
    18961 ttcacatatt ttggatgaat gatctggaga gccatcattg attaataaac actcaaaatt
    19021 ttgatacgtc tgattctgaa tgctatccaa acacattcgc aaataattct caacattgta
    19081 gattggaact acaatactaa ttagttcatt ttccatatac taactttcct agacttgatt
    19141 tattctattt ccctcttaat ctcttctctt tacaacaaga tttccattat tataaactac
    19201 actgtttgat ggaatatcat tgcgtatgag acaacctgct ccaataatgg tattatcccc
    19261 aatcgtcact ccttttaaaa tcgtgacatt acttcctatc cagcagtctc tgccaactct
    19321 aatcggcgct gtagtccact gccacttttc aatcttttca gcagtataaa tatggtcgtg
    19381 atcataaaaa cgaacaccct ctcccatcat ggtaccagct ccgatttcaa ttctttccat
    19441 acagttaaaa ctacaagagt tgttgataaa aacaccatca tgcagtatta acttaccaga
    19501 agagacatga aaattttcaa aatttctaca gatgacattg gctcctacaa tgaagtgagc
    19561 cttatcatcg attttaaaga cattccaagt cccatcttgg atgatttttt cttgatgatt
    19621 ggtgcctact ctgacttcat tcagatagtc tctcactcta gaaaccaatt tgtctggtgt
    19681 acttgataga aaaacttcct gcccctgatt tccatggact gtattgtcaa atgcaaagac
    19741 agattttcca gcctgtacaa acttggagac aatagcatct acttcttgaa agtgattgat
    19801 atctagtaaa aagtcacagg tatcgactag aaagtttagc tgttcttcat ttgtaatcgt
    19861 tcggagacgg acattatagt tcgtttctaa gctacggatt ttctcggaaa attgaacagg
    19921 tgctgcaata tgaaaacaga tatttggcaa ggattcaagg agataatcca actcttcaat
    19981 ctgatcacta accgtcaaaa tcagagcatc tcctactaaa tgatttttct tcatttgctt
    20041 atttctctca atttctcaat cattgcttga acttggtaaa cagcatatgc ctcctgacct
    20101 acttcatagg ttttagtatt ttcaaaggat aagataggct tgccaagatt agcaaattga
    20161 tcgagaattt cttctgtctt ttcgccatga ttaatatcta aaagtacttg actggtttct
    20221 accaattcat tatcgacatc taccaaatag tgaattccgt taaatatagt cacgtttgga
    20281 taaattgtca tctgagccaa tcgatcacta actattactc tagctgctat cttaaactga
    20341 atatcaggca aggattgaac caatgtctca atttgttcaa tatggtctga ggcagtatag
    20401 attagacaag tgaaaggttc ctttattgga tagatgtgag atctttgtaa tggatgtaaa
    20461 tgatggtttt gtcccaattc tgtccattca agcccatgat agtaccacca aacttcacga
    20521 taggtttggg ccgccaaatc tttccacggt ttccgatgag aaagatagtg aataatagca
    20581 ggataatcct gaccctcagg caattgataa tcagcgaact gtttatgaat gacaatatga
    20641 ttataatcaa agtccaattc caaccattta tgttcaaaaa gcatattcaa aatactttga
    20701 tcagcctgtt ccaccttatt atgccattca ttagtcaaat caattaattt ttgggtcata
    20761 ttctcttttt tccaaaaagc attgtttacc aagagaacac cggcattaaa gatttcttga
    20821 ccaaaataag ctctgccccc aaaatctcta acagcagcca aaggataatc ttgtaagtct
    20881 gtagcaaaca aatcatcaag atttttcgtt acaagtaaat cacagtccaa gtagagggcc
    20941 ttatcttctt gcacgaaatc agctatgaaa tagcgtaaaa aaactgtgta actaatatcc
    21001 gttttataac atgaaatttg ctcagaagtt acccgacaat taataatttc tgagtcaaac
    21061 ttctctaagc gcttatttaa ttgcttaatc cattcatttg gaaaatcgct atgaatcaaa
    21121 taaaaacgaa gcgagcgatt gtggtaacag atagacctga ttgtcgttaa aacttggtct
    21181 acataggcat aatttgccgc taggacaatg gctttctttt cacttgattc ttgtaccgtt
    21241 aactgattta aaagtctttt tttcatttca aactctttat acattgctgt gtcagataaa
    21301 ccactagctt aaccgttggc gagactgact tccaacatct gacgataaac tgccaagtgt
    21361 ttctctagag gatatcccat attagctagt agcgtaatac gttcagacat agcatcaact
    21421 aaggcgtgca tccacttttc tgtccaaact cttgatagac taccttttct aatccgataa
    21481 gcataaagac ctttatttaa ataaattacc ttttccgata ataaatatac cttttgattg
    21541 aggtaaccat cttctcctaa tttacccatg tcaaagcgca actgctcaaa caatctcgcc
    21601 ttatagagtt taccccaagc agatatcaaa gcgaaactcc tcatttcttg agtttcatac
    21661 aaattctcaa agatggaaac attgtcatat accttctcat aataggagtc tcctgatata
    21721 tgaaagtaga acattccttc actttcgttg aaagaatagt aattcccaac tgcaatatca
    21781 gcttgatatt ctgttatttt tttatataga gtttctacat aatcttgctc aatccaatca
    21841 tccgagtcca caaaggtcac ataatttccg gacatattat tcagaccggt gtttcgtgcg
    21901 gcagaaagac cagcattttc ttgttctata tagagaattc ggtgatccat ttctgaaaat
    21961 tctttacaaa tttcacctga agcatccgta gaaccatcat taacgacaac aatctcaata
    22021 tttttatatg tttaagaaat aatactatct agacatttcc tcagatagtt ttctacattg
    22081 tatactggta caatgactgt tattttatca tccactagca atcttctcct tgatttatgt
    22141 gcgcgagtta gtttagccta actttacagg ttgtcttact cttcttcatc acgtttacgg
    22201 cgtttcgcaa ccaatccaat acctgtaaca gctgctagaa ctccaagtaa cacagatcca
    22261 gttgacgact cagtacctgt attaggcaat tctttttggg atttacctgt cgatccagaa
    22321 gtatttccaa cttgtgagtt cgaatggttt gctgaatttg aaactgatgt acttgctgaa
    22381 gctgaggcac ttgttgacgc tgaagccgac gcactcgttg atgctgaagc cgaggcactc
    22441 gttgacgctg aagccgaggc actggttgac gctgaggccg acgcactggt gcttgctgaa
    22501 gctgaggtgc ttgctgaagc cgattcagac gctgaggtgc ttgctgaagc cgaagcactt
    22561 gttgatgctg aagctgacgc actcgttgag gccgaagctg aggcacttgt tgaggctgag
    22621 tctgaggcac tggttgatgc cgattcagac gctgaggtac ttgctgaggc cgacgcactg
    22681 gtacttgctg atgccgacgc act
//
LOCUS       DAVKQL010000036        21816 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVKQL010000036 DAVKQL010000000
VERSION     DAVKQL010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21816
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.036"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            22..1935
                     /locus_tag="VW561_001314"
     CDS             22..1935
                     /locus_tag="VW561_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HEU9943569.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNK
                     YKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
                     LLCIYSGGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKL
                     GTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEI
                     AKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKE
                     TQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLP
                     SKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEI
                     NRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDR
                     SVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSH
                     YHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNDQ
                     TGQQDTGDNDADY"
     gene            1922..3238
                     /locus_tag="VW561_001315"
     CDS             1922..3238
                     /locus_tag="VW561_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEU9943570.1"
                     /translation="MRITDNQYKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKILHINLTSLDVALSREERLYQLLVYLFPKYLQAAIRKGLYKEYHRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYVTREFTYDNPLMQLVRYTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLIFPSMEQSVNSEIGKV
                     AGYGAQLKKWSIRIPQNASFYSTFCKMMENSEENFKAIIDEEVGRK"
     gene            3413..4399
                     /locus_tag="VW561_001316"
     CDS             3413..4399
                     /locus_tag="VW561_001316"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEU9943571.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHDQGLIASISVGVKDYEY
                     DFVRQLKTDAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            4463..4534
                     /locus_tag="VW561_001317"
     tRNA            4463..4534
                     /locus_tag="VW561_001317"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4496..4498,aa:Arg,seq:ccg)
     gene            4717..5415
                     /locus_tag="VW561_001318"
     CDS             4717..5415
                     /locus_tag="VW561_001318"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEU9943572.1"
                     /translation="MKELQTVLKNHFAIEFTDKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            5406..8945
                     /gene="smc"
                     /locus_tag="VW561_001319"
     CDS             5406..8945
                     /gene="smc"
                     /locus_tag="VW561_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEU9943573.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLATLEDKRNS
                     LSKEKYDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDMHYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDAIAVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAEEEASLGSEEATLKTLQDQMARLSELLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEELDRLSDGDWQADKEKCQDRLATIASDKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLNIEQEEIQRMLQEKVDN
                     LEKVDTELLSQQAEESKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQNQLTDQYQISYTEALEKAHELENLNLAEQEVQDLEK
                     AIRSLGPVNLEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            8942..9736
                     /locus_tag="VW561_001320"
     CDS             8942..9736
                     /locus_tag="VW561_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9943574.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNEHVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKDQSVIAYMEGL"
     gene            9736..10554
                     /locus_tag="VW561_001321"
     CDS             9736..10554
                     /locus_tag="VW561_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9943575.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSNQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAVANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            10558..11847
                     /gene="ftsY"
                     /locus_tag="VW561_001322"
     CDS             10558..11847
                     /gene="ftsY"
                     /locus_tag="VW561_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEU9943576.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDIEPAFTEVEEVS
                     QEEAEVEIVEQAVFQEEEIQDTVEESQDSEPAVGVSQEEIEELPNSEELPEEEKLEHE
                     GTIEENNSEILEAERPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEE
                     LEELLIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDES
                     IHFQDNLTVMLFVGVNGVGKTTSIGKLAHRYKQAGKKVMLVAADTFRAGAVAQLAEWG
                     RRVDVPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGR
                     IIKRVVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAI
                     REELNIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(11893..13380)
                     /locus_tag="VW561_001323"
     CDS             complement(11893..13380)
                     /locus_tag="VW561_001323"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU9943577.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVCASWKLIDRIEKLWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDITYRQDGRLE"
     gene            complement(13529..14269)
                     /locus_tag="VW561_001324"
     CDS             complement(13529..14269)
                     /locus_tag="VW561_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9943578.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(14269..16434)
                     /locus_tag="VW561_001325"
     CDS             complement(14269..16434)
                     /locus_tag="VW561_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU9943579.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALSEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRIISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            16606..17559
                     /locus_tag="VW561_001326"
     CDS             16606..17559
                     /locus_tag="VW561_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9943580.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAYTLYGIG
                     LLALIGLARNFGEAGQNLATYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNFGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            17636..19624
                     /gene="uvrB"
                     /locus_tag="VW561_001327"
     CDS             17636..19624
                     /gene="uvrB"
                     /locus_tag="VW561_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEU9943581.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAVAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            19634..20197
                     /locus_tag="VW561_001328"
     CDS             19634..20197
                     /locus_tag="VW561_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9943582.1"
                     /translation="MIYLRKLKKEDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKETRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLVLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            20163..20651
                     /locus_tag="VW561_001329"
     CDS             20163..20651
                     /locus_tag="VW561_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9943583.1"
                     /translation="MTVIIKSMETPEEIEGKSLVHWQTWRESYDDLLPAEFQDTMTLE
                     RCRFFSQKYPENTLIAMDGVKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            20725..21189
                     /locus_tag="VW561_001330"
     CDS             20725..21189
                     /locus_tag="VW561_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9943584.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGTLQS
                     SEEGEVSWVQKDQIPNLNLAYDMLPLMEMMEAPDKSEFFYPRRTDLTFPSLR"
BASE COUNT         6759 a         3845 c         4817 g         6395 t
ORIGIN      
        1 ttagtaaaag gagttagatt gatgaataaa agaaaagtta gtttagaaga tttttataaa
       61 tggtatagtc taaataaaga agagttatta aataaggcaa ctgttggtga aaagtttaat
      121 gataaattaa aagaagagtt tctccaggaa tggcctttgg ataggatttt aacaatgtca
      181 atcgatgaat atgtaatagg aaagggacag caaaataagt ctttatgcta cgctcttgag
      241 aagggaaaat acaaaaatct atttcttgga atttctggtg gctcagcttc aaaatttggt
      301 atttattgga ataaaaaaac aaacaaatat aaagatcaag ctaataatga gatttcagag
      361 ttggatcagc gattttcaaa attaaaatca gatttgtatg aaattatcaa agaaggtatt
      421 cgttttaact ttgaaaatcc tatttttgat atgaaaagat caacaaatga atttattggt
      481 cgttctgcta tggtgacaaa attactttgt atctattctg ggggagatcc tttctttggt
      541 gtaaatatta atagtcagaa agaattttgg aaccactttg tttctcagac aaatcaaggt
      601 ggaccttatc tgcaaaatca taaaataatt gaactggtgt ccaaaactta tcctgagttg
      661 gagccatcga aattaggaac tatgcttttt gagtattcta agctttttat ggaaaataag
      721 gaagacaata gtacaatgga ttcatcaaac aattttcgtc atcaattaac tcaatctcta
      781 ttaaagtctc caaaccttat ccttcgtggt gctcctggca caggaaagac ttatcttgct
      841 aaagaaattg ccaaagaatt aacggatggc aacgaagacc aaatcggatt tgtacaattt
      901 cacccatcct atgattatac ggattttgta gaaggtttaa gaccagtatc aaatggggat
      961 ggagctattg agtttaggct acaggacggt atttttaaag atttttgtca gaaagcaaaa
     1021 gaaacccaat tgattggagg acaagataat tttgatgagg cttgggattc ttacttagaa
     1081 tatataaatg ttgctgaaga aaaagaatat ataacaaaaa catcttactt atctgttaat
     1141 agtagacaaa atttgtcagt aaattatgat agtggtgttc caggatggtc actacctagc
     1201 aaatatgttt acgagttgta taaagataaa aattataata agcaagaata ctacaaaagt
     1261 ggtggaaaaa ctgtcctaga aacattgaga aagagatttg gtttgaaaga ctatgtttcc
     1321 ccaacagaaa ttgatactga taagaaattc gtcttcatca tcgatgaaat caatcgtggg
     1381 gagatttcta agatttttgg cgaactcttt ttctctatcg accctggcta tcgtggtgaa
     1441 aaaggaagtg tttctaccca atatgcaaat ctacacgaaa ctgatgaaaa gttctatatc
     1501 cccgaaaatg tttacatcat cggaactatg aatgatattg atcgttcagt ggataccttt
     1561 gattttgcta tgcgtcgtcg ttttcgtttt gttgaagtta ctgccgaggg tcaagttggc
     1621 atgttggata aagagttgaa tatccatgca gaagaagcaa aaattcgtct aagaaacttg
     1681 aacgctgcta tcgaaaatgt tcaggaatta aacagtcatt atcatattgg accaagttat
     1741 tttcttaagt tgaaggatgt agattttgac tatgaattac tctggtctga ttatattaag
     1801 cctctcctag aagactactt gcgaggttct tatgatgagg ttgaaacttt ggaaactttg
     1861 aaaaaagcat ttgaactgac aaataatgat caaactggtc aacaagatac tggtgataat
     1921 gatgcggatt actgataatc aatacaagat tattaaagaa aaatttgttg aagaatatcc
     1981 taaactaagc aatcttcttt tagacagaac cttggaaagt ctatcccaag atgaacgtat
     2041 tttcattttt ccaaatgatt tgactcatac tcctgatttg gataaggacc aaaagatttt
     2101 tgaaacagtc aatcagaaaa tcaagacagg gaacgtgatt ggttttcttg gatatggtca
     2161 ggaaagatta acgatttcct cacgattttc tgatgagagt aatgaccatt ttttgcatta
     2221 tctcttaaac aagattcttc atatcaatct cactagttta gatgttgctt tgtctcgtga
     2281 agagaggctt tatcaacttt tggtgtatct ctttcccaag tatctacaag ctgctattcg
     2341 aaaaggtctt tataaggaat atcatcgatt ttctcataac gacagtcatg ttaagggagt
     2401 gattgatgta agaaaccatc tcaagaaaaa tcttcctttc acgggaaata ttgcctacgt
     2461 aacgagagag ttcacctatg ataatcccct catgcagttg gtccgttaca ctattgaata
     2521 cattaagaat cagaaaagca ttggtcaagg ggtactagat aatctctcaa ctagtcgtga
     2581 aaacgtatct gaaatcgtgc gtgtaacacc ctcttataaa ctagctgatc gtgctaagat
     2641 tattcgggga aatcaatcta aacctatacg tcatgcatac tttcacgagt acagaaactt
     2701 acaagaactt tgtctgatga tcctaaacca agaaaagcac ggtttagggt atcaagatca
     2761 aaaaatctat ggtattctct ttgatgttgc ctggctttgg gaagagtatg tttacacctt
     2821 gttgccaaaa ggttttgtac atcccagaaa taaggataag acggatggaa tttcagtatt
     2881 ttctgttggg aaacgaaaag tatatccaga tttttatgac agagaacgaa agattgttct
     2941 agatgcaaaa tataaaaaac tggaattgac tgaaaaagga atcaaccgtg aggacttatt
     3001 ccagctgatt tcctattctt atattttaaa agctgagaag gctggactga tttttcctag
     3061 tatggagcag tcagtaaata gtgaaatagg aaaagtagct ggctatggag ctcaattgaa
     3121 gaagtggtct attcgaatcc ctcagaatgc ctcattctat agtacatttt gtaaaatgat
     3181 ggaaaattca gaagaaaatt ttaaagcgat tattgatgaa gaagtgggga gaaaataatt
     3241 tcctctcttt cttttatttt taacacaagt aaaacccgaa caatatattt caattcataa
     3301 aataatttga caaaaactgt actttggttt ataataaatt aaggaaacgt cggaaaagac
     3361 gtagtgatgc gagagcatag gtaggtcatt acaaaagaaa cgagacatcg atatgttaaa
     3421 tgaatttcca atttttgatt acgaagatat tcaattgatt ccaaataaat gtgtcattaa
     3481 aagccgtgca gaagcggata caagtgtcac tttaggaaat cacaccttta aactacctgt
     3541 tgtgccagcg aatatgcaga cgattttgga tgaaaatgta gcagagcaac tggctaaagg
     3601 tggttacttc tacattatgc accgttttga tgaggcagga cgcattcctt ttattaaacg
     3661 aatgcacgat caagggctca ttgcttctat ctctgtcggt gttaaggact atgagtatga
     3721 tttcgttaga cagctcaaga ctgatgctcc tgagtatatc actattgata ttgctcatgg
     3781 tcatgcggat agcgtgattt ctatgattca acacatcaag aaagaattgc cagatacctt
     3841 tgtcattgct ggaaatgtgg gaacaccaga agctgtgcgt gaattggaaa atgctggtgc
     3901 ggatgctact aaggtcggaa tcggtcctgg taaggtttgt atcaccaagg ttaagactgg
     3961 ttttggtaca ggtggttggc agttggctgc tctacgctgg tgtgccaagg ctgcgcgtaa
     4021 accgattatc gctgatggag gaattcgtac tcacggtgat attgccaagt ctatccgctt
     4081 cggtgctagc atgatcatga ttggttccct ctttgcagga cacattgaaa gtccagggaa
     4141 aacgattgaa gtcgatggtg aacagttcaa agaatattat ggttcagcct cacaatatca
     4201 gaaaggtgct tacaaaaatg tggaaggcaa acgtatcttg cttcctgcta aaggtcatct
     4261 gcaagacact ttaactgaga tggaacaaga ccttcaaagt gctatttctt atgcaggtgg
     4321 acgtcaggtt gctgacctta aacacgttga ttatgtgatc gtgaaaaact ccatctggaa
     4381 tggggatgct tcccactaag acggggtctt tgactaaaaa acttgttatt agagcaaatt
     4441 tctgttataa taaaacaagt ttccaccctt agtgtaatgg atatcacgta agattccggt
     4501 tcttgagatg ggggttcgat tccctcaggg tggatgtaaa catcctaaaa agcctttaat
     4561 agggcttttt tctttacact gctctaaatt tgccgctaaa actgagaatc tctgtctaaa
     4621 gaattcccaa atgctttcca gtcccgtttt agagtgactc agggggcttt ttttgatata
     4681 ataaaaagga ctattatcag ttagaaagag gttggtatga aagaattaca aactgtacta
     4741 aagaaccatt ttgcaatcga atttacagac aaaaatttac tggaaactgc ctttactcat
     4801 acgagttatg ccaatgagca ccgcctctta aaaatttcac acaatgaacg cttggaattt
     4861 ttaggagacg ctgttctaca gttattgatt tcagaatatc tatataaaaa atatcctaaa
     4921 aagcctgaag gtgacctatc aaaactccgt gctatgattg tccgtgagga gagtttagct
     4981 ggttttgcgc gtgattgcca gtttgaccag tttatcaagt tgggtaaagg ggaagaaaaa
     5041 tctggtggtc gcaatcgtga caccattctt ggtgatgcct ttgaagcctt tcttggtgcc
     5101 cttcttttgg ataaggatgt ggccaaggtc aaggaattta tctatcaagt catgattcct
     5161 aaggttgaag caggcgagtt tgagatgatt acagactata aaacccatct ccaagagttg
     5221 cttcaggtca atggtgatgt ggctattcgt tatcaggtga tttctgaaac agggcctgct
     5281 cacgataagg tctttgatgt agaagttctt gttgaaggta agagcatcgg tcaaggccaa
     5341 ggtcgttcta agaaattagc agagcaggaa gctgccaaaa atgccgttga gaaggggctg
     5401 gattcatgta tttaaaggaa atcgaaattc aggggttcaa gtcttttgct gataagacca
     5461 aggtcgtttt tgaccaaggt gtgacggcag ttgttggacc caatggatct ggaaagtcca
     5521 atattacaga aagtctgcgt tgggctttgg gggagtctag tgtcaagagt ctccgtgggg
     5581 gcaagatgcc ggatgtcatc tttgctggaa ccgaaagtcg caaaccgctc aattatgctt
     5641 ctgtagttgt gactctggat aatcatgacg gatttatcaa ggatgcaggt caagaaatca
     5701 gggtggaacg ccatatctat cgtagtggag atagcgaata caagattgac ggcaagaaag
     5761 tccgtctgcg tgatattcat gacctcttct tggatactgg attgggacga gattccttct
     5821 ctatcatttc ccaagggaag gttgaggaga tttttaattc taagcctgag gaacgccgag
     5881 ctatttttga agaagctgca ggagttttaa aatacaagac tcgcagaaaa gaaactgaga
     5941 gtaaactgca acaaactcag gataatctag accgcttaga ggacattatc tacgagttgg
     6001 ataatcaaat caagcctctt gagaagcaag ctgagaatgc ccgtaagttt ttagacttgg
     6061 aaggacaacg taaggctatt tatttagacg ttctggttgc tcaaatcaag gaaaataagg
     6121 cagaactaga gtcgacagaa gaagagctgg ctcaggttca agaactcttg atgagttatt
     6181 accaaaagcg tgaaaaatta gaagaagaaa atcaaactct taaaaagcaa cgccaagatt
     6241 tacaggctga aatggccaaa gaccaaggca gtttgatgga cttgactagt ctgattagtg
     6301 atttagaaag aaaattagcc ctatcgaaac tggagtccga gcaagtagcc ctgaatcaac
     6361 aggaggcaca agctcgtttg gctactttgg aggataagag aaattcactc agcaaagaaa
     6421 agtatgataa agaaagctct ttagctctgt tagagggaaa tctagtccaa aataatcaaa
     6481 aactcaatcg tttagaagct gaattgctgg ctttctcaga cgatcctgat cagatgattg
     6541 agctcttacg tgaacgcttt gtagctcttt tacaagaaga agcggatgtc tcaaaccagt
     6601 tgacccgtat tgagaatgag ttggaaaata gtcgtcagct ttctcaaaaa caagcagatc
     6661 aactagaaaa gctgaaagag caattggcta tagctaaaga gaaggctagt cagcaaaagg
     6721 aagagcttga aactgccaag gagcaggttc agaaattatt ggctgactac caagctattg
     6781 ccaaggagca agaggagcag aaaacttcct atcaagctca acaaagccaa ctctttgacc
     6841 gtctggataa tctcaaaaac aagcaggcca gagctcaaag tttggaaaat atcctgagaa
     6901 atcatagtaa cttttatgca ggtgttaaga gtgttctcca agaaaaagat cgcctaggtg
     6961 ggattattgg tgcagtcagt gagcatctga cctttgatat gcattatcaa actgccctag
     7021 aaattgcgct tggagcgagt agccaacata tcatcgtaga agatgaagag tcggcaacca
     7081 aggctattga cttccttaaa cggaacagag ccggtcgtgc aacctttctt cctttgacca
     7141 ctatcaaggc gcgtacgatt tctagtcaga accaagatgc tatcgctgta agcccaggtt
     7201 tccttgggat ggcagatgag ttggtgactt ttgatactag actggaagcc attttcaaga
     7261 acttgctagc tacgacggct atctttgata ctgtagaaca tgcgcgtgca gcagcgcgcc
     7321 aggttcgtta tcaggttcgt atggtgacat tggacggaac agagctgcgc acaggtggtt
     7381 cttatgcagg tggagctaat cgccagaata acagtatttt catcaagcca gaactggagc
     7441 aattacaaaa agaaattgct gaagaagaag caagcttggg ttcagaagag gcgactttga
     7501 agaccttaca agaccagatg gctagattgt ccgaattatt agaagccatc aaatctcaag
     7561 gagaacaggc acgtattcag gagcaaggct tgtccctcgc ttatcagcaa actagtcagc
     7621 aagttgaaga actggaaact ctttggaaac tccaagaaga ggaattagat cgtctttctg
     7681 acggagattg gcaagcagat aaggaaaaat gccaagatcg tcttgctact atcgccagtg
     7741 acaagcaaaa tctggaagct gagattgaag agattaagtc taacaaaaat gccatccaag
     7801 aacgctatca aaacttgcag gaagagctag cgcaagctcg tttgcttaag acagaactgc
     7861 aagggcaaaa acgttatgaa gttgctgata ttgaacgctt aggcaaggaa ttggacaatc
     7921 ttaatatcga acaagaagaa attcagcgca tgctccaaga aaaagttgac aatcttgaga
     7981 aggttgatac agaattgctc agtcaacagg cggaagaatc caaaactcag aaaacaaatc
     8041 tccaacaagg tttgattcgc aagcagtttg agttggatga tatagaaggt caactggatg
     8101 atattgccag tcacttggat caagctcgcc agcagaatga ggagtggatt cgcaagcaaa
     8161 cacgtgctga agccaagaaa gaaaaggtca gcgagcgctt gcgccatcta caaaatcaat
     8221 taacagacca gtaccagatc agctacacag aagctttaga aaaggcacat gaattggaaa
     8281 acctcaatct ggcagagcaa gaggtgcagg atttagagaa ggctattcgc tcattgggac
     8341 ctgtcaactt ggaagctatt gaccagtacg aagaagttca caaccgtctg gactttctaa
     8401 atagtcagcg agatgatatt ttgtcagcga aaaatctgct ccttgaaacc attacagaga
     8461 tgaatgatga ggtcaaggaa cgctttaaat caacctttga agctattcgt gagtccttta
     8521 aagtgacctt caagcagatg tttggcggag gtcaggcaga cttgatattg actgagggcg
     8581 accttttaac agctggtgtg gagatttctg ttcaacctcc aggtaagaaa atccagtcgc
     8641 ttaacctcat gagtggtggt gaaaaagctc tatcggctct tgccttgctt ttctccatta
     8701 ttcgtgtcaa gaccattcct tttgtcatct tggatgaggt ggaagctgcg ctggatgaag
     8761 ccaatgttaa acgttttggg gattacctca accgctttga caaggacagc cagtttatcg
     8821 tcgtaaccca ccgtaaggga accatggcag cggctgattc catctatgga gtgaccatgc
     8881 aagaatcggg tgtttcaaag attgtttcag ttaagttaaa agatttagaa agtattgaag
     8941 gatgacaatt aaactagtag caacggatat ggacggaacc ttcctagatg ggaatggacg
     9001 ctttgatatg gatcgtctca agtctctctt ggcttcctac aaggaaaaag ggatttactt
     9061 tgcggtggct tcgggtcggg gatttctgtc tctggaaaaa ttatttgctg gtgttcgtga
     9121 tgacattatt ttcatcgcgg aaaatggcag tttggtagag tatcaaggtc aggacttgta
     9181 tgaagcgact atgtctcgtg acttttatct ggcaactttt gaaaagctga aaacgtcacc
     9241 ttatgtagat atcaataaac tgctcttgac gggtaagaag ggttcatatg ttctagatac
     9301 ggttgatgag acctatttga aagtgagtca gcattataat gaaaatatcc aaaaagtagc
     9361 gagtttggaa gatatcacag atgacatttt caaatttaca accaacttca cagaagaaac
     9421 gctagaagct ggtgaagctt gggttaatga acatgtccct ggtgttaagg ctatgacaac
     9481 tggctttgaa tctattgata ttgttctgga ctatgtcgat aagggtgtag ctattgttga
     9541 attagctaaa aaacttggta tcacaatgga tcaggtcatg gcttttggag acaatcttaa
     9601 tgacttacat atgatgcagg ttgtgggaca tcctgtagct cctgaaaatg cacgacctga
     9661 aattttagaa ttagcaaaga ctgtgattgg tcaccataag gaccagtcgg ttatagctta
     9721 tatggagggc ttataatggc agatataaaa ttaatcgcat tggacttgga cgggaccttg
     9781 ctgactactg ataaaaggct gacggatcgt accaaggaaa ccttgaaagc tgcgcgtgat
     9841 cgtggtatca aggtcgtatt gacaactggt cgtcccttaa aagctatgga tttctttctc
     9901 catgagctag ggactgacgg tcaggaagat gagtatacca ttacttttaa tggtggtttg
     9961 gttcagaaaa atacaggtga aatccttgat aagacagtct tttcatatga tgatgtggca
    10021 cgcttgtatg aagaaacaga gaaattatca ctgcctcttg acgctatctc agaaggaact
    10081 gtttatcaaa ttcaatcgga ccaagaaagt ctttatgcca aattcaatcc agctttgact
    10141 tttgttccag ttgattttga agacctgtct aatcaaatga cctacaacaa atgcgtgacc
    10201 gcctttgctc aagaaccctt ggatgcagcc attcagaaga tttctccaga attgtttgac
    10261 caatatgaaa tctttaaatc acgtgaaatg ttgctagaat ggtcaccaaa gaatgttcat
    10321 aaagcaacag gtttggcaaa actaattagc catcttggaa ttgatcaaag tcaagtgatg
    10381 gcctgtggtg acgaggccaa tgacctctct atgattgaat gggcagggct cggtgttgcc
    10441 atgcaaaacg ctgttcctga agtcaaggca gtcgcaaatg tggtaactcc tatgaccaat
    10501 gatgaagaag ctgtcgcctg ggctatcgaa gaatatgtgc taaaggagaa ctaagatatg
    10561 ggattgtttg accgtctatt cggaaaaaaa gaagaaccta aaatcgaaga agttgtaaaa
    10621 gaagctctgg aaaatcttga tttgtctgaa gatattgagc ctgccttcac agaagttgag
    10681 gaagtttctc aggaagaagc agaggttgaa attgttgaac aagctgtgtt ccaagaagag
    10741 gaaatccaag acacagttga agaaagtcag gattcagagc cagctgtagg ggtttctcaa
    10801 gaagaaatag aagaattacc aaactcagaa gaattaccag aggaagagaa gcttgagcac
    10861 gaaggaacta tagaagaaaa taattctgaa attcttgaag cagaaaggcc tcaaacagaa
    10921 gaaactgttc aggaaaaata tgaccgcagt cttaagaaaa ctcgcacagg tttcggtgcc
    10981 cgcttgaatg ccttctttgc taacttccgc tctgttgacg aagaattttt cgaggaactg
    11041 gaagaactgc tgattatgag tgatgttggt gtccaagtcg cttctaactt aacggaggaa
    11101 ctacgttacg aagccaagct tgaaaatgcc aagaaacctg atgcacttcg tcgtgtcatc
    11161 attgagaaat tggttgagct ttatgaaaag gatggtagct acgatgaaag catccacttc
    11221 caagataact tgacagttat gctctttgtt ggtgtgaatg gtgttgggaa aacaacttct
    11281 atcggaaaac tagcccaccg ctacaaacaa gctggtaaga aggtcatgct ggttgcagca
    11341 gataccttcc gtgcgggtgc agtagctcag ctagctgaat ggggccgacg agtagatgtt
    11401 ccagtagtaa ctggacctga aaaagctgat ccagccagcg tggtctttga tggtatggaa
    11461 cgtgccgtgg ctgaaggtat cgatattctc atgattgata ctgctggtcg tctgcaaaat
    11521 aaggataacc ttatggctga gttggaaaag attggtcgta ttatcaaacg tgttgtgcca
    11581 gaagcaccac atgaaacctt cttggcactt gatgcatcaa caggtcaaaa tgccctagta
    11641 caggccaaag aattttcgaa aatcacacct ttaacgggaa ttgttttgac taagattgat
    11701 ggaactgctc gaggaggtgt ggttctagcc attcgtgaag aactcaatat tcctgtaaaa
    11761 ttgattggtt ttggtgaaaa aatcgatgat attggagagt ttaactcaga aaactttatg
    11821 aaaggtctct tggaaggttt aatctaatca gaagcaaaaa tcctgcaagg cataaacttg
    11881 caggaaattt ttttattcta agcgaccatc ttgacgatag gtgatatctg gttgccaagt
    11941 ccatttggca ccgaattttt caagtaggtc aaagctggct tgaggtccca tgcttccagc
    12001 tttatagtca tgaagtgggg caccattttc agcccagagc ttttcaatac ggtcaatcaa
    12061 cttccatgac gcacaaactt catcccagtg gctaaagtta gttgagttgt tatttaggac
    12121 atcataaatc aatttttcgt atggttctgg agaagcacca gttgcagtcg catctgtacg
    12181 gtaatcaagt gagttaggag ccaagttaaa ttcttctcct acttgcttcc catttaggct
    12241 aagagagaag ccttctgttg gttgaatata gatggtcaaa atatttggag caagtggttc
    12301 tccaaagata gaatccattt gtttaaagac gatgttgaca tgagttcctt tttcagtcag
    12361 tcgtttacct gtacggaaaa agaaaggaac accacggaat cgatcgctgt ctacaaagaa
    12421 ggcaccagat gtaaaggttt cagttgttga ttctggattc acatttggct cgctacgata
    12481 agagatgtat ttcatgccat caatcttacc agagcggtat tgcccacgga taaagtgttc
    12541 tttgagttct tcgtcagttg gatgatagag gtttttaaag accttaatct tttcagcacg
    12601 aatctcgtct tttgtgaagc ttgctggttt gtccatggcg aggagcgaaa gaagttgtag
    12661 agtgtggttt tggaccatgt cacggagggc accggattgg tcatagtagc caccacgttc
    12721 ttctacaccc aagcgctccg caaaggtaat ttgaacattg tcgataaaat ccttgttcca
    12781 aacgttttca aaaatcaagt ttgcaaagcg aactgcaaag atgctttgga tcatttcctt
    12841 accaagataa tggtcgatac ggaaaatttg ttcttcgtca aatgttgcta ggagttcgtc
    12901 attcaacttg cttgcagttg cgtaatctgt accaaatggt ttttcaacga tcaagcgctc
    12961 aaaacctttg ccatcgacaa tgttttcaga tttgaggtgt ttggcaatgg ttccaaagaa
    13021 ctgaggtgcc atagacaaga agaagagctt attgtgttca gcttggtatt tttcattaag
    13081 ctcagcttgt aattgacgca aagcaatata atgttccgaa tcattgacat catggctttg
    13141 atagtagaag tggctagcaa attcttgggc ttgctcggta ctatctgcca aatcaaggat
    13201 ggactcgaca actacagatt caaaatattc cttactccaa ggtctacggg cagttccaat
    13261 aacggcaaag tgcttggaaa gattgccgga tttatatagt ctaaaaaggg aagggtagag
    13321 cttgcgttta gccaggtctc cactcgcacc gaaaattgta acaataacct tagatgacat
    13381 ctagctacct aatttctatt tttattccta gtcttgctag gtatgaggaa acctcatttc
    13441 ataaacttaa ttctaacata attttccgaa aaatcaaaaa ttccaatacg aagtagaaaa
    13501 actgcaagga aatccttaca gtttgagttt agacgtttaa gaccttatct aagaactctt
    13561 tcagacgagg gtgttgtggg ttatcaaaga tttggtcagg tgttccgtct tcaaggaact
    13621 cgccatctgc agtaaagata acgcggttgg caacctgacg agcaaatccc atctcatggg
    13681 ttacgataat catggtcatg ccttgctcag ccaattcctt cataacgtta agtacgtctc
    13741 caaccatctc agggtcaagg gcagaagttg gttcatcgaa gagcatgatg tctggattca
    13801 ttgctaggcc acgagcgatg gccacacgtt gtttttgacc acctgatagg ctatctggat
    13861 tggcattagc tttatctgct agtccaacct tttcaagcaa ctccattccc aattcctcag
    13921 cttcttcctt agtcatcaac ttgtgctcaa taggagcaaa ggtgatgttg tccaatacag
    13981 acatatgagg gaagaggttg aagtgttgga ataccatgcc gatattttca cggacgtggt
    14041 caacattggt tgttttttca gttaaatcat agccgttcac agtgatgtga ccgctagtga
    14101 cttcttctaa aagattgagg ctacggagga aagttgactt accagaacct gaaggaccga
    14161 tgatacaaac aacatctcct tcatagaact tagtcgtaat tccttttagg acttcatttt
    14221 ttccatagtg cttgtgtaaa tcatttacat caatttttaa ttttgccatt aacgaatcct
    14281 cttttctaag cgtttcgcta gtctagtcaa aagtgtgata attacaagat agaagatagc
    14341 aaggattgca tacatcttga aactttggta gttacgagca atgataatct taccagtttg
    14401 gaagagttca accaaaccga tagcagatac gatagttgta tctttaagag cgataacgaa
    14461 ttggttgaca aagtttggca acatcaattt agttgcttgt ggcaagataa tcttacgcat
    14521 ggtttttcca taagagatac ccaagcttcg gctggcttcc atttggccaa ctggaacggc
    14581 ctgaatacca ccacgaacga tttcagcgat ataagcagcc gcattgagtg agagggcaat
    14641 ggttccagct acaaagtcgt taattgggct ttgttggcct gtgatagact cgatgaagtt
    14701 tggaattccc cagaagatga aggctgcaag aatcatcaat ggaataccac gaataacgtc
    14761 aacgaaaatc tcagagatga tgcgaagaga tttgtatggg ctaacgctaa acataccgaa
    14821 gataattccg atgacaatgg caatagcaaa tgagataaga gctagagcaa gagtgatacc
    14881 aagaccgcta aggagttgtt tgtagttgtt ttgaagcaag ccccagagcg ttgtttcgtc
    14941 aacagtactt gttgaagcag ttgaagattc gctagctagg tatttgtcaa gaatcttttg
    15001 gaattcaccg tttgctttaa ggtttgcaag tccgttgttg aacatttcaa tcagttctgg
    15061 atttgctcct tttttaacag caaaggctgt ttcaccgatt ggagttccag agattggagt
    15121 tttcaatttt tgaccttggc tgatagaata tttgagaaca ggttcatcat ccataacggc
    15181 atcaatggca ccagtgttta aactgtcata cattgaagaa ccatcagcaa aggttttgat
    15241 tttgtaaccg tatttgcttt gattttctgt taggaaggtt tgagaagcag ttccgttttt
    15301 aacaccgact gtctttcctt ttagatcttc ataagaagca atattgcttg attctttgac
    15361 accaagaatg gtattagcag tgtagtatga ttctgagaag tcaaaagttg ccttacgagc
    15421 atctgtgaca gacataccag caatgatacc atcggcttga ccagcttgga cagcactgat
    15481 agcagcatca aaaccagggt tggtgatttc aatttcaaaa ccttggtctt tagcgattgc
    15541 cttaatcaat tccatatcaa taccagtgta ttggttgctt gaattttgga aaacaaaagg
    15601 tgcaaaagaa gaatcgctgg caatgatata tttagcctta acaggaatag cttttaatcc
    15661 tgcgagagta gttgtagttg gcactgctga agatgatgaa gcagtccatt tcttgataat
    15721 tttatcaaga ctaccatctt ttttcatttc agacaaggct tggttaaatt cagtaaccag
    15781 gtgctcgtat ttacttcctt ttttcacacc gaaagcaaaa cttcctacag cttcaccatc
    15841 catttcaata tggaggtctt gaccttggtt aatggcatat tcgataacag gtttgtcatc
    15901 catcatggca tcgatggcac cagcactcaa gctgttgttc attaaatcac cagtgtcaaa
    15961 tgttttaata gtaaagccgt atttatcttt gattgtttca aggaaacgtt gagcggcagt
    16021 tccgttttta acaccaacgg ttttgccagt taattggtcg tacttgctaa ttttgtgtga
    16081 ctttgtagta gcaatgacaa cttttgtatc atagtaagta tcagacatgg tgaagacttt
    16141 ttcacgttct ttagtctttg tcatccctgc catgatagcg tcggcttgcc cagcttgaac
    16201 cgcattgact gctgcgtcaa atccaggata ggacatctga atgttccagc ctttaatctc
    16261 agcgactttg ttaataatgt caacatcaat tcctttataa gtttgatctg aatctttaaa
    16321 ctcaaaaggt gcataggcag tatcagaaac aatcttaatc gtttctgctt tggcaatacc
    16381 taatgagaaa attgggaata aaattagcaa aaatgctaga aattttttct tcatttttag
    16441 tctccttttc cgaatattct ctcattatat cagaaaaagt aaaaaaaggc aaatgttata
    16501 tttttcgtgt cagaaaatat aacattgatt tttccttttc tttcttgaat tgctattaaa
    16561 aagtgctata ataatagtat atatagaaga aaagaggacg gggatatgaa gaacaaaaga
    16621 atatttaaag acttccaagc ttcaaaaatg agtttaaaca tttacacaag ccccttgtta
    16681 gcctttgttt ttgtcttcat aggagagttt gtggcttata ctttgtatgg tattggcttg
    16741 ttagctctca tcggacttgc tagaaatttt ggagaggctg gtcaaaatct tgcaacctac
    16801 ttgcagacct tgcatcagag cttgacggat aaaacaagtg actttcgttt aattttagga
    16861 ttactggcct ttggttttat tcttaacact gtgttcagat ggacaagaaa agttgagaaa
    16921 agacctattc gaaccttggg attttataga gagaatttcc tcagcaatct tctgaaagga
    16981 tttagtctag gtctggcact ttttcttctg accttgttag gtttagtggt cttaggtcaa
    17041 tatcgtttgg aatccattca cttgaatcct tattctcttg cctttgtcgt ctttactatc
    17101 ccattttgga ttttacaggg gacagcagaa gaagtggtgg cccgtgcttg gctacttcct
    17161 caattggcct caagaaccaa tctaaaacta gctattctta tatctagcct gttctttacc
    17221 ctgcttcata tgggcaattt tggcctcacc cctctatctc tagtaaatct ctttttattc
    17281 ggagttgcca tggctcttta ccttctcaaa actgatacag tttggggtgt tgcaggtatt
    17341 catggtgctt ggaattttgc tcagggtaat ctctttggta ttttagttag tggtcaaccg
    17401 tcaggaacgt ctctgatgac ctttttacca caaggcaatc aagattggct atcaggtggt
    17461 tcttttggca tagaaggttc cattatgaca agtctggtat tactactgct gattgtctat
    17521 cttgctaata aattaaagaa agaaaatgaa aggatgtgac ttcggtccgt ccttttcttc
    17581 gtgaaaatac tataagtatg ctaaaatagg aatagcacat ggagagagga ttcttatgat
    17641 caatcacatt acagataatc aatttaaact agtatcaaaa tatcaaccat caggagatca
    17701 accccaagct atcgagcagt tggtggataa cattgagggg ggagaaaaag ctcagattct
    17761 gatgggggcg actggaacag ggaagaccta tactatgagt caggtcattt ctaaagtcaa
    17821 taaaccaact ctggttattg cccacaataa aactctggct ggtcagctct atggggagtt
    17881 taaggaattt ttccctgaaa atgcagttga gtatttcgta tcctactatg attattacca
    17941 gccagaggcc tatgtccctt ctagcgatac ctatattgag aaggatagtt ctgtcaatga
    18001 cgagattgac aagcttcgcc actcagctac ctcagccctt ttggagcgta atgatgttat
    18061 tgtcgtggcc tcagtctctt gtatctatgg tttgggttcg cccaaggaat acgctgatag
    18121 tgtcgttagt ctccgtcctg gtctagagat ttctcgtgat aaactcttga atgacttggt
    18181 cgatattcag tttgaacgta atgatattga tttccaacgc ggaagatttc gcgttcgtgg
    18241 ggatgtggta gagattttcc cagcttcccg agatgaacat gcctttcgag tagaattttt
    18301 tggagacgaa attgaccgta tccgtgaggt tgaggctctg acaggtcagg tattgggaga
    18361 ggtggatcat ttggcgattt tcccagctac tcactttgtg accaatgacg accatatgga
    18421 agttgccgtt gccaagattc aagcagaatt agaagagcag ttagctgtct ttgagaagga
    18481 aggtaaattg cttgaagccc agcgcttgaa acaacggaca gagtacgata tcgaaatgct
    18541 gcgcgagatg ggctatacaa atggggttga aaattattct cgccacatgg atggacgtag
    18601 tgaaggtgag cctccttata cgcttctcga ctttttccca gatgattttt tgattatgat
    18661 tgacgagagt catatgacta tgggacaaat aaaaggcatg tacaatggag accgttcgcg
    18721 taaggaaatg ctagtaaatt atggtttccg tttaccgtct gccttggaca atcgtcctct
    18781 ccgtcgtgag gagtttgaaa gccatgtgca tcagattgtt tacgtatcag cgacacctgg
    18841 tgactatgaa aatgaacaga ctgagacggt gattgagcaa atcattcgac cgacagggct
    18901 tttggatcct gaggtggaag tccgtccgac tatgggacag attgatgatc tcttaggtga
    18961 aatcaatgcc cgtgttgaaa agaatgaacg aacctttatc accaccttga ccaagaaaat
    19021 ggcagaggac ttgaccgact atttcaagga aatgggcatc aaggtcaagt acatgcactc
    19081 ggatatcaag accttggaac ggacggagat tatccgtgat ctccgcttgg gtgtctttga
    19141 tgtcttggtt ggaatcaatc tccttcgtga agggattgac gttcctgagg ttagcttggt
    19201 tgccattctc gatgctgaca aggaaggttt ccttcgtaac gagcgtggac tcatccagac
    19261 cattgggcgt gccgcccgta acagtgaagg tcatgtcatc atgtatgcag atacggttac
    19321 ccagtctatg caacgtgcta tcgatgaaac tgcccgccgt cggaaaattc agatggccta
    19381 taatgaagag catggtatcg taccacagac aatcaagaaa gaaatccgtg acctgattgc
    19441 cgtgaccaag gcagttgcta aggaagagga caaggaagtc gacatcaata gcctcaacaa
    19501 acaagagcgc aaagaactcg tcaagaaact agaaaaacaa atgcaagaag ccgtcgaagt
    19561 gcttgacttt gaactggcgg ctcagatccg tgatatgatg ttggaagtca aggccttgga
    19621 ttaggggaat agtatgattt atttaagaaa gttaaagaaa gaagatttga tgtctttatg
    19681 ggaaatggct tattcacagc ttaatccagt ttggaaacag tatgatgctc cctattatga
    19741 tgattatcag tatttttcaa attttaaaga attcgaacta caaaaatcag aatccatttt
    19801 aagcaactca aatcgccttg gtatttttgt tgatgataaa ctagttggga ctgtttcgcg
    19861 ttattgggta tgtaaagaaa caagatggat ggaattggga attggtattt atgataaaaa
    19921 attctggaac actggtattg ggaaagttgc tatgttgcag tggatagata ggacgtttca
    19981 ggattacttg gagttggagc atctggtttt gacaacttgg tcaggaaatc ttggtatgat
    20041 gaaacttgct gaaaaattaa gaatgaaaaa agaagctcat attccaaaag ttcgttatta
    20101 tcaaggtaaa tattttgata gtattaaata tggtattttg agagaagact gggagaaaat
    20161 aaatgacggt tattatcaaa tcaatggaaa ctcctgaaga gatagaaggc aaatccctcg
    20221 ttcactggca aacgtggaga gagtcttatg atgacctttt gcctgcggaa tttcaggata
    20281 caatgacatt agaaagatgt cgattcttta gtcaaaagta tccagaaaat acattgattg
    20341 cgatggatgg cgtgaaggtg gttggtttta tcagttatgg caattttcgt gatgagacta
    20401 ttcaagctgg tgaaattatt gctttatatg ttttaaaaga ctattatgga aaaggtatcg
    20461 cacaaaagtt aatgaaagca gctttgactg ctcttaatca tttttctgaa attttcttat
    20521 gggtattgaa agataacaag cgcgccattg ctttctatca aaaaatgggt tttacttttg
    20581 atggacaaga aaaaatactt gaacttggaa agcctataaa ggaaaaacga atggtgttct
    20641 attctaaata attttaaaaa agtaaaagat aatatctctg gtagtaagtc tgaaaattta
    20701 ataaattaga aagcgagtaa atttatgtcc cgttcccaat taacaatttt aacaaatatc
    20761 tgtctgattg aagacctcga aactcagcgc gtggtgatgc agtatcgcgc ccctgaaaac
    20821 aatcgctggt ctggttatgc ctttcctgga ggtcatgtag aaaatgatga ggcttttgcg
    20881 gagtctgtca ttcgtgaaat ctacgaagaa acagggttga ctatccaaaa tcctcaactt
    20941 gtcggcatta aaaattggcc actagataca ggtgggcgct atattgtcat ttgttataag
    21001 gcgactgagt tctctggtac ccttcaatct tcagaagagg gagaagtttc ttgggtgcaa
    21061 aaagaccaga ttccaaactt aaatctggcc tatgatatgc taccattgat ggaaatgatg
    21121 gaagctcccg acaagtcaga gtttttctat cctcgccgta cagatctaac tttcccatct
    21181 ttgagatagc ccttcacaag atagacatga gtacgccttt ctttgttgtt ttaataaacg
    21241 ctctactcca ttactccata tataaccaat atacaacaaa tagttgggaa caaactgtgg
    21301 tagatgtcga acgtcttcgt gatgaggcta tagctctagg agtagcttaa tgacaaagag
    21361 acagtaggag aggtacgatt atgttactct ttatgttttc gttcaataat ataataataa
    21421 atcctagtat gttcctattg ttcgatagta caggagctta ctatcggcac atgttgagac
    21481 ggtagtattg atgtcaaaag ttgtaccaac taagtaaata aagtgtagaa attatggctt
    21541 attctaaggc tatcgttaaa aggtatcggt agtttggagt aagccttgtt tttttattta
    21601 tcttgccaag attacaggat tagttgcagg tgtcttcaga gatatatttg taggaaggga
    21661 tttgttttta attaaaacat aattctatta cccagatata ttttgaatta atatgttcac
    21721 aatagtgaaa aacaagcaat acacagagtg gaggtgaaaa tatctgaaat ttgttcaatc
    21781 ttaataaatt ttctgatatt tttccgaaat ttatat
//
LOCUS       DAVKQL010000037        20975 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVKQL010000037 DAVKQL010000000
VERSION     DAVKQL010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20975
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.037"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            112..3213
                     /locus_tag="VW561_001331"
     CDS             112..3213
                     /locus_tag="VW561_001331"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU9943585.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDISLKLPRFNPARPAVEELRERAELGFVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLDLFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDSALEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSIEAGHTAEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNYSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKSIYIGLKSIKGLSKDLALWIIEHRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
     gene            3296..4303
                     /gene="pfkA"
                     /locus_tag="VW561_001332"
     CDS             3296..4303
                     /gene="pfkA"
                     /locus_tag="VW561_001332"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEU9943586.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            4362..5867
                     /gene="pyk"
                     /locus_tag="VW561_001333"
     CDS             4362..5867
                     /gene="pyk"
                     /locus_tag="VW561_001333"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU9943587.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(6086..6379)
                     /locus_tag="VW561_001334"
                     /pseudo
     CDS             complement(6086..6379)
                     /locus_tag="VW561_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167986.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(6545..6770)
                     /locus_tag="VW561_001335"
                     /pseudo
     CDS             complement(6545..6770)
                     /locus_tag="VW561_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7618..8490
                     /locus_tag="VW561_001336"
     CDS             7618..8490
                     /locus_tag="VW561_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEU9943588.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(8623..8980)
                     /locus_tag="VW561_001337"
                     /pseudo
     CDS             complement(8623..8980)
                     /locus_tag="VW561_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            9123..9434
                     /locus_tag="VW561_001338"
     CDS             9123..9434
                     /locus_tag="VW561_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943589.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEHPELYAVAQTSGEIPEKECEELRQIITTIFEQKMNKHSI
                     K"
     gene            9438..10154
                     /locus_tag="VW561_001339"
     CDS             9438..10154
                     /locus_tag="VW561_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9943590.1"
                     /translation="MKKYFIGGLGSNAYHSKDFLQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEEFICLIGHSLGGDLARYLASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIVLDLDVLTSKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNEATWREEALKELPDYFDTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(10283..11098)
                     /locus_tag="VW561_001340"
     CDS             complement(10283..11098)
                     /locus_tag="VW561_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEU9943591.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWITAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(11095..12000)
                     /locus_tag="VW561_001341"
     CDS             complement(11095..12000)
                     /locus_tag="VW561_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEU9943592.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSIVVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSHLSPAKKVFQVFVQAIDEFFDTGRYLVFGCLFASVIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLAYSLLIGVIL"
     gene            12412..14541
                     /locus_tag="VW561_001342"
     CDS             12412..14541
                     /locus_tag="VW561_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEU9943593.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
     gene            14528..14977
                     /locus_tag="VW561_001343"
     CDS             14528..14977
                     /locus_tag="VW561_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEU9943594.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPIKLYRCQSCQQTYQRKRRIDTKRYRCGLCRGKLLLVNQPED"
     gene            15035..15307
                     /locus_tag="VW561_001344"
     CDS             15035..15307
                     /locus_tag="VW561_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU9943595.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVLIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(15355..15519)
                     /locus_tag="VW561_001345"
     CDS             complement(15355..15519)
                     /locus_tag="VW561_001345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943596.1"
                     /translation="MDEAGEILLILFKNQSANYINFTLPTKLQPAAGFLVCTLIFIEY
                     KSKKALNQAV"
     gene            complement(15661..15852)
                     /locus_tag="VW561_001346"
     CDS             complement(15661..15852)
                     /locus_tag="VW561_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943597.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFISTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            16280..17038
                     /locus_tag="VW561_001347"
     CDS             16280..17038
                     /locus_tag="VW561_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9943598.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPTRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFNEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            17040..19028
                     /locus_tag="VW561_001348"
     CDS             17040..19028
                     /locus_tag="VW561_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9943599.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTITYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVLFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQMKVVITVLL
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVTDPLTALTTFFLAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYKIKEKEVLRYTYFAVANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFNKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVDASEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSNLADASSQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(19070..19714)
                     /locus_tag="VW561_001349"
     CDS             complement(19070..19714)
                     /locus_tag="VW561_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEU9943600.1"
                     /translation="MLRAGVLGANDGIISIAGVVIGVASATTNIWIIFLSGFAAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQNGECKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSEYR
                     IPATVLIVGVALLLTGYTSARLGKDPTRTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(19686..20735)
                     /locus_tag="VW561_001350"
     CDS             complement(19686..20735)
                     /locus_tag="VW561_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9943601.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASLLGKAPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVKKSSLTKELKEKILHYHNQ
                     KFSPEMMVIAKGVNVGISTIYYWIHRGKLGLSKQDLLYPRKGKALKKQASTNFKPAGQ
                     SIEQRPEAINLRLENGHYEIDTVLLTRAKNYCLLVLTDRKSRHQIIRLIPNKSAEVVN
                     QALKLILKQHKILSITADNGTEFNRLFDVFSEEHIYYAHPYASWERGTNENHNRLIHR
                     WLPKGTKKMTPKEVAFIEKWINNYPKKCLNYKSPREDFLMANLNLKFSVRNKSRNRFK
                     FCRSFKYPARRCSWC"
BASE COUNT         6371 a         3825 c         4438 g         6341 t
ORIGIN      
        1 atacaaatga atagaaaata aattagtaag ctgatgaaat ttttctcaag agaagccatt
       61 tataggtgaa aatggtataa tatagtgaga aggatagagg agaagtgtaa attgatcgca
      121 caactagata caaaaacagt ctatagtttt atggaaagcg tcatttcgat cgaaaagtat
      181 gtgagagcag ctaaagaata cggctacact catttggcta tgatggatat tgacaatctt
      241 tatggcgctt tcgactttct agagattaca aaaaaatacg gcattcatcc tttgctaggg
      301 cttgaaatga cagtgtttgt agatgatcag gaagtgaatt tgcgcttttt agctctatct
      361 agtgtgggct atcagcagtt gatgaagctt tcgacagcca agatgcaggg ggagaaaact
      421 tggtcagtcc tgtcccagta cctggaggat atcgcggtca ttgtgcctta ttttgataga
      481 gttgagtcgt tagaactagg ctgtgattac tatatagggg tttatccaga aacactagca
      541 agcgaatttc atcatcctat cttacctctt tatcgggtca acgcttttga aagcagggat
      601 agagaagttc ttcaagtttt aacagcgatt aaagaaaatc taccgctcag agaagttccc
      661 ttgcgttcga gacaagatgt ctttatatca gcaagttctt tagagaaact attccaagag
      721 cgttttccgc aagctttgga caatttagaa aagcttattt caggcatttc ttacgacttg
      781 gatattagtc tgaaactgcc tcgttttaat ccagctagac cagcagtaga ggagttgaga
      841 gagcgtgctg aactggggtt tgttcagaag gggttgacta gtaaagaata tcaagataga
      901 ctagaccaag aattgtctgt tattcatgat atgggctttg atgattattt cttggttgtt
      961 tgggatttgt tgcgttttgg acgatcgaat ggctattata tgggaatggg aaggggttct
     1021 gcagtaggca gtttggtttc ttatgcctta gacatcacgg ggattgaccc agtagagaaa
     1081 aatctgattt ttgaacgctt tcttaatcgt gaacgctata ccatgcctga tattgatatt
     1141 gatatcccag atatttatcg tccagatttt atcagatatg ttggtaataa atatggtagt
     1201 aaacatgcgg cacaaatcgt tactttttca acctttggag ccaagcaagc tcttcgagat
     1261 gtcttgaaac gctttggtgt gccagagtat gaattatctg caattactaa gaaaatcagt
     1321 tttcgtgaca atcttaagtc ggcctatgag ggcaatctcc agtttcgtca gcaaatcaat
     1381 agtaagttag aataccaaaa agcttttgag attgcttgca agatagaggg ctatccaagg
     1441 caaacctctg tccatgcggc tggtgttgta attagtgacc aagatttaac caactacatt
     1501 cctctaaagt atggtgatga aattccactg actcagtatg atgctcatgg agttgaggct
     1561 agcggacttt tgaagatgga ctttctggga ctacgaaatt tgacctttgt ccagaagatg
     1621 caagagttgc ttgctgaaac agaaggtatt caccttaaaa ttgaagaaat agatttggaa
     1681 gacaaagaaa cgttagattt atttgcctct ggaaatacaa aaggtatctt tcaatttgag
     1741 caacctggtg ccattcgctt gctcaaacgt gttcaaccag tctgttttga agatgtcgta
     1801 gcaactactt ctctaaatcg accaggtgca agtgactaca tcaataattt tgtggcaagg
     1861 aagcatgggc aggaagaagt gactgttcta gattcagctc tggaggatat tttggctcca
     1921 acttatggca ttatgctcta tcaggagcag gttatgcagg ttgctcagcg atttgctgga
     1981 tttagtcttg ggaaggccga tattttgcgt cgagctatgg gtaaaaagga tgcctctgct
     2041 atgcatgaga tgagggcttc ctttattcaa ggctccatag aagcaggcca tactgcggaa
     2101 aaagcagagc aggtttttga tgttatggag aagtttgcag gttatggatt taatagatct
     2161 catgcctacg cctactcagc cttggccttc cagttggctt atttcaaaac gcattatcca
     2221 gccatttttt atcaggtcat gttaaattat tccaacagtg attacttaat agatgcactt
     2281 gaagcaggtt ttgaagtagc ctctctatcc atcaatacta ttccctatca cgataaaatt
     2341 gccaacaagt ctatctatat aggtttgaaa tcgattaagg ggctcagcaa ggacttggcg
     2401 ctttggatta ttgaacatag accttattct aacattgaag attttatagc taaattacct
     2461 gagaattatc tgaaacttcc tctgctagaa cctttggtaa aagttggtct tttcgattca
     2521 tttgaaaaaa atcgtcaaaa agtatttaat aacttagcta atctatttga atttgtgaaa
     2581 gagttgggaa gtttgtttgg agatgctatt tatagttggc aggaatcgga agattggacg
     2641 gaacaagaaa aattttatat ggaacaagag cttttaggga taggtgtcag caaacatcca
     2701 ctacaagcta ttgcaagtaa ggctatttac ccgattaccc caatcggaaa tttgtcagaa
     2761 aatagctatg ctattatttt ggttgaagtt cagaaaataa aagtgattcg taccaaaaag
     2821 ggtgaaaata tggccttctt acaggcagat gatagtaaga aaaaattgga tgtcactctc
     2881 ttttcagact tatatcgtca ggttggacag gaaataaaag agggagcctt ctactatgta
     2941 aaaggaaaaa tacaatcacg tgatggccgt ctgcaaatga ttgcacaaga aataagagaa
     3001 gcagttgctg aacgcttttg gatacaggtg aaaaatcatg aatcggatca agaaatttca
     3061 cgcattttag aacaatttaa aggcccaatc ccagtcatca tccggtatga agaggaacag
     3121 aaaaccatcg tttctcccca tcattttgta gctaaatcca atgaattaga ggagaaattg
     3181 aatgaaatcg ttatgaaaac gatttatcgc taaaaatacg gaaaatagaa gaattttaaa
     3241 atcaaatgtg atataatcaa gaagaatgtt aaaagaaaaa ggagcataaa ccaatatgaa
     3301 acgtattgct gttttgacta gtggcggaga cgcacctggt atgaacgctg ccatccgtgc
     3361 agttgttcgt caagcaattt cagaaggaat ggaagtgttt ggtatctatg acggatatgc
     3421 tggtatggtt gccggtgaaa ttcatcccct agatgcagct tcagtagggg acatcatttc
     3481 tcgtggtggt acttttcttc actcagctcg ttatccagag ttcgctcaac ttgaagggca
     3541 acttaaaggg attgagcaat tgaaaaaaca cggaattgaa ggtgtagttg ttatcggtgg
     3601 tgacggatct taccacggcg ctatgcgttt gactgaacat ggcttcccag ctattggtct
     3661 tccaggtaca atcgataacg atatcgttgg tactgacttt acaatcggtt ttgacacagc
     3721 ggttactact gccatggacg ctatcgataa gattcgtgat acatcatcaa gtcaccgtcg
     3781 tacttttgta atcgaagtta tgggacgtaa cgctggtgat atcgctcttt gggctggtat
     3841 tgcaactggt gctgatgaaa tcatcatccc tgaagcaggc ttcaagatgg aagatatcgt
     3901 agcaagcatc aaagctggtt atgaatgtgg taaaaaacac aatatcatcg tcttagctga
     3961 aggtgtgatg tcagcggctg aatttggtca aaaacttaaa gaagctggag atacaagcga
     4021 ccttcgtgta acagaacttg gacatattca acgtggtggt tctccaactg cgcgtgaccg
     4081 tgttttggcg tcacgtatgg gtgcacatgc tgttaaactt cttaaagaag gtatcggtgg
     4141 tgttgcggtt ggtattcgta acgaaaaaat ggttgaaaat ccaattcttg gtactgcaga
     4201 agaaggggca ttgtttagcc ttactgcaga aggtaagatt gtggttaaca acccgcacaa
     4261 agccgatatt gagctatcta gcttgaataa gagcttgtca taatttacaa tttagttaat
     4321 aagaccaaaa aggtcatata tataaaggag tcacaaaaat catgaacaaa cgtgtaaaga
     4381 tcgttgcaac tttgggtcct gcggtagaaa tccgtggtgg taaaaaattc ggtgaggatg
     4441 gatactgggg tgaaaaactt gatgttgaag cttcagctaa aaacattgct aaattgattg
     4501 aagctggtgc taacacattc cgattcaact tctcacacgg cgaccaccaa gaacaaggtg
     4561 agcgtatggc aactgttaaa cttgcggaaa aaattgcagg taaaaaagtt ggtttccttc
     4621 ttgatacaaa aggacctgaa atccgtacag aattgttcga aggtgaagct aaagaatatt
     4681 catataaaac tggtgaaaaa attcgtgttg caactaaaca aggaatcaaa tcaactcgtg
     4741 aagtgattgc gttgaacgtt gctggtgctc ttgatatcta tgatgatgtt gaagttggtc
     4801 gtcaagtttt ggttgacgat ggtaaacttg gtcttcgtgt ggttgctaaa gatgatgcaa
     4861 ctcgtgaatt tgaagttgaa gttgaaaacg atggtatcat cgctaaacaa aaaggtgtga
     4921 acatccctaa cactaaaatt cctttcccag ctcttgctga acgcgataac gacgatatcc
     4981 gtttcggtct tgaacaaggt atcaacttca tcgcaatttc attcgtacgt actgcaaaag
     5041 atgtgaacga agttcgtgca atctgtgaag aaactggaaa cggacatgtt caattgttcg
     5101 ctaaaatcga aaaccaacaa ggtatcgata acttagacga aattatcgaa gcagctgatg
     5161 gtattatgat tgctcgtggt gatatgggta tcgaagtacc gttcgaaatg gttccagttt
     5221 atcaaaaaat gattatcaag aaagtcaatg ctgcaggtaa agttgttatc actgcaacaa
     5281 acatgcttga aacaatgact gaaaaaccac gtgcaactcg ttcagaagta tcagacgtat
     5341 tcaacgctgt tatcgacgga actgacgcta caatgctgtc aggcgagtct gcaaacggta
     5401 aatacccact cgagtcagta actacaatgg ctacaatcga caagaacgct caagctcttc
     5461 ttaatgaata cggacgtctt gattcagatt catttgagcg taactctaag acagaagtaa
     5521 tggcttctgc tgttaaagat gctactagct caatggatat caaattggtt gtaactctta
     5581 ctaagacagg tcatactgca cgtttgattt ctaaataccg tccaaatgct gacatcttag
     5641 cattgacatt tgacgaattg acagaacgtg gcttgatgtt gaactggggt gttatcccaa
     5701 tgttgacaga tgctccatct tcaactgacg atatgttcga aatcgctgaa cgtaaagcgg
     5761 tagaagcagg tctcgttgag tcaggcgatg atatcgttat cgttgctggt gtgccagtag
     5821 gagaagctgt tcgcacaaac acaatgcgta tccgcacagt acgttaagaa aaatataaaa
     5881 acctatcata tctagctttc tagcctgtat gatgggctct ttttttgtcc agggggcaaa
     5941 actttcataa tggattgata ctcttcgaaa atcaaattca aaccacgtca gcgtcgcctt
     6001 accgtactca agtacagctt gcggctagct tcctagtttg ctctttgatt ttcattgagt
     6061 atgaaataag atatgcacaa attgattaga aagtcaaatg aatttctaca aatgttttag
     6121 caatcgtaat gtacttgtct agattcgatc tgatatattt tcgatttaat gatatggtat
     6181 ttaaaacctc caaagtagct tactccattc ttttacttac gtgagtgtag atgttattta
     6241 ctgttttagc gtttttgtgt tccactctaa ccattatagc attcttctca gctagtgtac
     6301 taaggagtgt gtgcctgaaa atatgggaac taaggggctg gtttatcggt ttctctagtt
     6361 tagtatttgc cttttgcaaa gtgatgttaa atgcctttct ctaaatttac atatcactat
     6421 tgtttaacaa aatctaatct attttaggtc acttattctt tttttgaaat gtagaatgaa
     6481 ctttttcaaa gtttttcgaa tcttttaaaa tctgtttgtt ttatatcgcc attctccccc
     6541 ttttttaatt ctccctatat agcctgacaa ctttcccgat ggtacgaata tggttgcttt
     6601 cgtctaggtg gatgtcgggg tatttgggat tgagtttttt taaggtagcc ttggcggagt
     6661 ttcttgacat agttagcgcc gtctacttgg aagatgccga tggtattata gtcaatctgt
     6721 ggggtattct tgataaatag gtagtcgctg tttcttatct ttggctccat ggacttgctg
     6781 acgacataag cgattgggtc gtagtcgtct gggataatgg aaactccata tctaaatcgt
     6841 tgtcctgcat cgagcggcta cctgcagaga taaactacct aacacgagag taagtagtct
     6901 gtctgtagtc gtccagtctg atgattttta cgatacttcg tttttctgat catacagttg
     6961 cctctcggca taggtcagaa ctttaccttg tctgggtggt gcccgttggt cgtagataga
     7021 ttggatatcg ctaggagaat ccttttgaac tggagaaaag agggcatcaa tcaagctact
     7081 gaatacttta actaagtcaa atatagtatt tttcttagta gacctaaccc ttttttcata
     7141 atttctaatg gtgtttttac ttatacctat cttagtatcc aattcttatt gagtccaacc
     7201 attactagtc tatattgttt tatagttgat tgagtttgga atagtacgct gtagctgcta
     7261 aaacatttct aggaattaat ttgcctttcc taatagagtt gttcatatct tatttcaatc
     7321 tattatgttt ttcacctcta acaatcgcaa tctcttcttt atctacgaat gaaatcgctt
     7381 tctatttttg taagtaaagc atcacacgaa atccacgaaa atgaaaacct ttgttgtgtt
     7441 ttcgtaaaaa atttgttgac agagcacgaa acgcctacta taattaaatc aagcttaagg
     7501 aaataacaaa aaaacaaatc ggagggaaag acgatgaaca cattaaatga gaaagtaatc
     7561 aatatctgta aagcagtagt taaagaaact ttaatccaag acatttagga gaaagaaatg
     7621 aagaaatcaa gaaaattagc cacattagga atctgttcag ctttgttttt aggcttggct
     7681 gcttgtcaac aacaacatgc tacttctgag gggacgaatc aaaggcaaag cagttcagcg
     7741 aaagttccat ggaaagcttc atacaccaac ctaaacaacc aggtaagtac agaagaggtc
     7801 aaatctctct tatcagctca cttggatcca aatagtgttg atgcattttt taatctcgtt
     7861 aatgactata ataccattgt cggctcaact ggcttatcag gagatttcac ttcctttact
     7921 cacaccgaat acgatgttga gaaaatcagt catctctgga atcaaaagaa gggcgatttt
     7981 gttgggacca actgccgtat caatagttat tgtcttttga aaaattcagt caccattcca
     8041 aagcttgaaa agaatgacca gttgcttttc ctagataatg atgcgattga taaaggaaag
     8101 gtctttgatt cacaagataa ggaagagttt gatattctat tttcgagagt tccaactgag
     8161 gcaactacag atgtcaaggt tcacgctgaa aagatggaaa cattcttctc acaatttcaa
     8221 ttcaatgaaa aagctcgaat gctgtctgta gtcttgcacg acaatctgga tggcgagtat
     8281 ctgtttgtag gccacgttgg ggtcttagta cctgctgatg acggtttctt atttgtagag
     8341 aaattgactt tcgaagagcc ctaccaagcg attaaatttg ctagtaagga agattgctac
     8401 aagtatttgg gcaccaagta tgcggattat acaggcgagg gactggctaa gccttttatc
     8461 atggataatg ataagtgggt taaactttaa tagtgttatg atatgacagt aatcttagga
     8521 tttttatttc tatttttgat gagttggtgg gttatttcaa tcgttaatag ccagaaacca
     8581 cgcaaaaatg agacatttat tggctatgtc caaagatacg atattgatgg gaatgtctat
     8641 caatcattac cgtgtcctca gaactgagag gagttcttga aatcgtaaaa ctactttgaa
     8701 caagagttac ttcaacccat tggctccgac ggagtaagtt gctttcgtgg ataccaaaaa
     8761 cagccgcaat ttgttcataa gtgcggtatt ctcgcacata ttgaagagtg gccataagaa
     8821 ggtcttctag gcttaattta ggttttcgtc ccccttttgt gtgtttaagt tgataagcag
     8881 tttttaatac agctaatatc tcttcaaaag tggtgcgctg aacaccaaca agacatttaa
     8941 atcgtgcatc agttagttgt ttacttgctt cataattcat agaactacta taccatattt
     9001 tgtttcgcag gaagtctaat gctaaaatct tatcagtgag aagatttctt aaagtttatt
     9061 ctttgtaatt tcatctgtat tagtacataa tttaacttag agaaaatagg aggactaatc
     9121 gaatggaatt aaaagatttt acagaaaaag aacaggaaat gattaagaaa aggcttacaa
     9181 tgtctaatat tagtgacaaa gaaactactg agaagattct ggcgctagta ccacaagact
     9241 tgattaagcg aatcccgttt tttgtcagaa aacatgctac aacacgtacg attaaacgca
     9301 tttcaattga acaccctgaa ctctacgctg tagctcaaac aagtggtgag attccagaaa
     9361 aagaatgcga agaattgcgt cagattatca caactatctt tgaacaaaag atgaataagc
     9421 atagtattaa gtagagaatg aaaaaatatt ttattggcgg tttgggaagc aatgcctatc
     9481 atagcaagga ttttcttcaa gaactagatt cgcaggtcta ttttctaaat ccatatgaaa
     9541 agcatcttcg agatgaaaca gaattgaaat catggtttaa aaatgagatt gtagaggaag
     9601 aatttatctg tctgataggt cattctcttg gaggagattt agctcgttat ctcgcatcgg
     9661 aatttgaaga agtaaagaaa ctgattcttt tggatggtgg ctatctagat ttagataaga
     9721 ttttaccttt ggatacagag ttagaggaaa ctaaaaatta tatcaaatct caaattgttt
     9781 tggacttaga tgttcttact tctaaagaaa aatctgaagc aaagcattgg tcagaaaata
     9841 tggagaaagc tgtaagacag tcctatcact ggaatgttga gtataataga tatgagttgg
     9901 ctataaatta tgaaaatata gaagcgatac tccgcctacg gagaaaaata caagctttta
     9961 agagagaagt gggagatacc ttgtttatta gtcctcgcta tcctaatgaa gctacatgga
    10021 gagaggaagc cctaaaagaa ttgccagact attttgatac tattttttta gaaaactttg
    10081 gccatgagct ttatactcaa gcacctaaag aaatcgctag tctaatgaat gagtggctcg
    10141 cttattttct atgagctact tgtattgctg gaaatggtat aagatgaaaa tctacaagtc
    10201 actaattttc atataacaaa aaacgaacag gctctttttc tgttattcag aagagaactt
    10261 gttcgttttt atcttgattt tcttaaaaag ctctatatac atagggattt tctggtttat
    10321 cgactttggt aaagctgtcg atttccaagg ttggaaggtt ttgtttgagt tctttatggt
    10381 agtgattgac cagttttcct ttggctgtta tccaagtgtt gttttcatac tgccgggtat
    10441 tgcccttggt cagcaatcca tagacaccag aatctgcgat acagtggata atgccgaatc
    10501 ggaacagaaa ttgactattg gcatgactgg ggtcgttata gacaaagcct gtaaactgga
    10561 ttgtcttgcc ctcaaactca tctggatagt cgtagatagc ctccatgact tccatatagt
    10621 tttcattagt gatctgaata ctatcttggg ataagtattt atccgccgcc gttcgcattt
    10681 ccttttcata ggctgatttt gaaaaataag aactggtatc tggtttcaaa tattggcttg
    10741 tcgtcccttc gcttgtctga atggctagat ccgttccttc cgataggggg aaatgataac
    10801 ctttagcaga aacagtctga gaatccaagc taacagttgg gaaagttaag ccgatgacaa
    10861 gtggaatagc cagaagagaa atactcgtca tcttggctaa tcggctgttc agatgactgt
    10921 gggttttgac ttgcttcatc cagatataca attgaacgat agccaagata aaagaaagca
    10981 ccatggagat ataggccaga taggaatagt gcatgttgat gtactggttt agtttgcccg
    11041 acagatggag gtaaatagtc agttcaaaat agccagctaa aactaaaaat cgaatcatag
    11101 gatgactcca atcaagagag aataggctaa aacgaccaga gtcacaatcg taatgaagtg
    11161 actaataaat cgtgctttca agtaattttt catcataaga atatttttga tatccagcat
    11221 tgggccaatg acgagaaaag ccagaactgg agccaaaccg aaactcgaga gtagagaagc
    11281 cccgataaag gcatccgcct cactacagag cgaaagaaga aaggccaaaa tcatcaagag
    11341 caggatggca aaaagaggag tcgcactgat agaggtcaga atccgagtcg gaacataaac
    11401 ctgtataaca gaggcaaaga gacagccaaa taccaaataa cgtcccgtat caaaaaactc
    11461 atcaatggct tgcacaaaga cctgaaaaac tttttttgca ggacttaagt gagaaaaatc
    11521 atgctcatga caggctagac gattttcttt ttgaatcggt tcttgccaga aaaatcctag
    11581 aaaaattcct agtattacag ccacaacgat ggaacccaga gctcgtaata gggcgacatg
    11641 gaaggagttg ccaaaggcag aataggtcgc aaaaagaaca atgggattga taacaggtgc
    11701 tgtcacaaga aaaggaacgg ccgtgtaact tggaaccttt ttttccagaa aacgattgat
    11761 gatggggacg attccacatt cacaggaagg gaaaagtata ccgacaaagg tcccaaaaaa
    11821 gattctcccc caacgatttc gagggagaaa atgataaacc ttgtcaggtg tgatataaac
    11881 ttcaatcagc cctgagacaa tacttcctat cagaacgaag ggaagggctt caatgataat
    11941 ggagagaaaa atggctccag tttgaagtac actagatggg agttgttgaa aagcagtcat
    12001 ctagtttttc ttttctgctt tggctttttt ctctttgtca tcagggaaag tgacttgttt
    12061 caaatcaggg agtttagcaa actcttcaaa catcttgtcc aagtcgtctc gttttgtaaa
    12121 tttgacagcc ataggtgtac ctcgattcta aattctacct ctattatact attattttgc
    12181 ggaaaaggga gttttttaat ttgttttcga tagttgatac tcttcgaaaa tctcttcaaa
    12241 ccacgtcagc ttcgccttac cgtagtacgg ttactgactt cgtcagttct atccacaacc
    12301 tcaaaacagt attttgagca gcctacggct agcttcctag tttgcttttt gatttttatt
    12361 gagtatgaaa aggattggag aaagccttcc agcttgtggt ataatagatt tatggataaa
    12421 aaatatgaaa aaatctctca ggatttggga gtgacgttaa agcaaattga taccgttcta
    12481 agtttgacag ctgaaggggc gactattccc tttatcgcgc gttatcgcaa ggacatgact
    12541 ggtagtctgg atgaggtggc gattaaggct attattgatt tggataaaag tctgacaaat
    12601 ctcaatgacc gtaaggaagc tgtcttagct aagattcaag aacaaggtaa gttgaccaag
    12661 gaattggaag aagctatctt agttgccgaa aaattagcag acgttgaaga actctatctt
    12721 ccttataagg aaaagcgtcg taccaaggca accattgccc gtgaagctgg actctttcct
    12781 cttgctcgct tgattttgca gaatatagtt gacttagaga aagaagctga aaagttcgtc
    12841 tgtgaaggat ttgcgactgg caaggaagcc ttgaccggtg cagttgatat tttggtcgaa
    12901 gctttatcgg aagatgtgac cttgcgttct atgacttatc aggaagtgct gagacactct
    12961 aaactcactt ctcaagccaa ggatgaaagt cttgatgaaa agcaggtttt tcagatttat
    13021 tatgattttt cagagacagt tggaactatg caaggctatc gtaccttggc tctcaatcgt
    13081 ggggagaaac ttggtgtctt gaagatcggt tttgaacatg cgacggaccg tattcttgcc
    13141 ttctttgcta ctcgtttcaa ggtgaaaaat gcttatattg atgaagttgt tcagcaatcc
    13201 gttaagaaaa aggtcttgcc tgctattgag cgtcgtattc ggacagaatt aactgagaaa
    13261 gctgaagagg gagctatcca acttttttct gacaatctgc gcaatctcct cttggttgct
    13321 ccactgaaag ggcgcgtggt tcttgggttt gacccagcct ttcgtacagg tgccaagcta
    13381 gctgtcgtgg atgcaacagg aaaaatgctg acaactcagg ttatttatcc tgttaaacca
    13441 gcatcagctc gtcaaatcga agaagccaag aaagatttag cagatttaat tggtcaatac
    13501 ggtgtagaga ttattgccat tggaaatgga acggccagtc gtgaaagtga agcttttgta
    13561 gcggaagttc tgaaagattt ccctgaagtc agctatgtta tcgttaatga aagtggtgct
    13621 tctgtctatt ctgccagcga acttgctcgt caggagtttc cagacttgac cgttgaaaaa
    13681 cgctctgcca tttctatcgc ccgtcgtttg caagatcctc ttgcggaatt ggtcaaaatc
    13741 gatcctaagt caattggtgt cggtcaatac caacacgatg tcagtcagaa gaaactctct
    13801 gagagtctgg actttgttgt cgatacagtg gttaaccaag ttggtgtcaa tgtcaataca
    13861 gctagcccag ctcttctttc acacgtagct ggactcaata aaaccatttc tgaaaatatc
    13921 gtcaaatacc gtgaggaaga gggaaaaatc acttcacgct cccaaatcaa gaaagttcct
    13981 cgtttggggg ccaaggcttt tgaacaggct gctggtttcc tccgtatccc tgaaagtagc
    14041 aatatccttg ataatacagg agttcaccca gaaaattacg ctgccgttaa ggaactcttc
    14101 aaatgcttgg acattaagga tctgaatgaa gaggcccaaa gcaaactcaa gtcgctttca
    14161 gtcaaggaaa tggcgcaaga gttagacctt ggtccagaaa ctcttaaaga tatcattgca
    14221 gatcttctca aaccaggtcg agatttccgt gattcctttg atgctccagt tctccgtcaa
    14281 gatgtcctag atatcaagga cttagtggtt ggacaaaagc tagaaggagt ggtgcgtaac
    14341 gttgttgatt tcggtgcttt tgttgatatc ggaattcatg aggacggtct catccatatt
    14401 tcccatatga gtcgcaagtt tatcaaacac cctagtcaag tggtggcagt tggagatttg
    14461 gtgagcgttt gggttaagaa aattgatact gaacgtgaaa aagttaatct gtctctccta
    14521 gctccaaatg aaactgactg attacgtcaa gcaggtttca ctagaagact tcggcagacc
    14581 ttttatccat catgtccagt ggaataggcg tctacgttcg acaggtgggc gatttttccc
    14641 caaagatggg catttggatt ttaatcccaa ggtttatcag gaactggggt tggacgtttt
    14701 taggaaaatt gtccgacatg aactctgtca ttatcacctc tattttcaag ggaaaggcta
    14761 tcaacacaag gatcgggatt ttaaggaact gttgaaagca gtggatggat tacggtttgt
    14821 accatccttg cccaatagta actccaagcc aatcaagctc tatcgttgcc aatcctgcca
    14881 gcaaacttat cagcgtaagc gtaggattga taccaaacgc tatcgctgtg gactttgtcg
    14941 aggaaagttg ctattagtaa atcagcctga ggactgatga aagccgagcc tatccgtgat
    15001 atactagtct aacctagcag aaagaggaaa tatcatgaac agtaagtttt ataaaatgag
    15061 acgaaatcgc atgatttcag gagttttagc aggtctgtca gacaagtgga actttgatgt
    15121 tacccttgta cgttttctgt ttgccatttt tacagtcgca aactttggac ttggggtgct
    15181 tatctatatc atcctagctt ctatcatgcc aaccaaggaa gaaatcgaag cagaaatgta
    15241 cggaacaggc ccacgtaaac gcaaagaagc tcaagccatt gacgataatg aaggctggtt
    15301 ttggtgaggt tttacaaaag atttggaata cgcaaaaagc tttaaatcaa tagtctaaac
    15361 tgcttgattt aaagcttttt tacttttata ctcaataaaa atcaaagtgc aaactaggaa
    15421 gccagccgca ggctgtaatt tagtcggcaa tgtgaagttg atatagtttg cactttgatt
    15481 tttgaatagt attaataata tttcgccagc ttcatccaca aactatagtg gattggaact
    15541 agaataggac acctatactt ctaaaaaatt gttagaaatc gatttgactg tcctgatcga
    15601 tttgtcctgt tcttatttca ttttactata aaagcagtaa tttaaacgca gttttttaat
    15661 ctatttttgc tgttgagaca atacattttt ggaaaaatgg tggatgctga taacaactga
    15721 taaccagcaa ataacataag cagtgccggt aaattctgta ctgataaagt gtgtgtgttc
    15781 aactaagagc tgaccgatga atggagagag cagatagaga agtccgatga gtatatgttc
    15841 tttgtgtttc attaaagcgc accttctttc cttataggtt gaacgacttg caagtgttaa
    15901 attgaatggt atgttatcta ggtaagctta ctaaaatgtt agttcccgct tcctcctttt
    15961 caaagtaagc cttttcacac ttatatttta caactttaaa aataggaaat caaatcattt
    16021 tctaagtaat tttgcttata tttcacccaa tcttacaaaa atgtaagatt aaagtctctt
    16081 ttgtaaggtt aggttatggc aagcggtctt tttttgatgt aaactagact cataaagaac
    16141 aaaggagtaa catcatgaaa caaatcttac ataagaaaac tagaaaacca agtcacaaag
    16201 aaattgagcg cgttcagctg ggatgcacta tgatgcaagc tacatttcat ttgatgggat
    16261 attaagaaag gagattctca tgacactttt agatgtaaaa cacgttcaaa aaatttataa
    16321 aacacgtttt cagggcaacc aagtagaagc cctcaaggat attcacttta ccgtagaaaa
    16381 gggtgactac gttgccatca tgggtgagtc tggttctggt aaatcaactc ttctcaatat
    16441 tctagctatg ttggataaac caactcgtgg gcaggtttac ttgaatggaa ctgacaccgc
    16501 aactattaaa aattcacagg cttctagttt ccggcgtgaa aagctaggat ttgtcttcca
    16561 agactttaac ttgctagata ctctgtctgt taaggacaat atcttgcttc cgcttgtctt
    16621 gtcaagaaga cctataacgg agatgatgaa gaaattggtg gtgacagctg agaatctggg
    16681 tattaaccaa ttgcaagaga agtaccctta cgagatttct ggtggtcaga aacagcgtgt
    16741 agcagtagcc cgcgccatca tcacagaacc tgaaattctc cttgcggacg agccaacagg
    16801 agcccttgat tccaagtcat ctgcagcctt acttgatgtc tttaatgaaa tcaatgagcg
    16861 tgggcaaacc atcctcatgg taacccactc aacagcagct gctagcaggg ccaagcgtgt
    16921 tctctttatc aaagacggca ttctttacaa ccaaatctac cgtggagaga agacagagcg
    16981 tcagatgttc caagaaatct ctgatacctt gactgtcatg gcaagcgagg tgaattagta
    17041 tgtttcgatt aaccaataag ttagcggtat cgaacttgat taaaaaccgc aaactctact
    17101 atccctttgc actggctgtt ctcttggcag tcaccatcac ctatctcttt tactccctaa
    17161 ccttcaatcc aaagattgcg gaaatccgtg gaggaaccac cattcaagca acacttggat
    17221 ttggtatgtt tgtcgttacc cttgcgtcag ccattatcgt cctctatgcc aatagttttg
    17281 tcatgaaaaa ccgttccaag gaactgggta tatatggcat gttaggcttg gagaagcgcc
    17341 atctaatcag tatgaccttt aaggagttag tgctatttgg gattctaact gttggagcag
    17401 gaatcggtat tggagccttg tttgacaagt tgattttcgc tttcctactc aaactgatga
    17461 aattgaaggt ggaactggtc gctaccttcc agatgaaagt tgtcattaca gtacttcttg
    17521 tcttcggttt gattttccta ggcctcatgt tcctgaatgc ccttcgaatc gcccgtatga
    17581 atgccctcca gctctcacgt gagaaagcta gcggagagaa aaaaggtcgc ttccttcctc
    17641 tccaaacgat tcttggttcc ataagtttag ggattggcta ttatcttgcc cttacggtaa
    17701 ccgatcctct tacagcccta acaactttct tcctagctgt tttactggtt atctttggta
    17761 cttatctttt gttcaatgca gggattaccg ttttccttca aatcttaaag aaaaataaga
    17821 aatactatta ccaacctaat aatctcatat ctgtttctaa cttgattttc cgtatgaaga
    17881 aaaatgcagt tggactagca actatcgcta ttttgtcaac aatggttttg gtaaccatgt
    17941 cagcagcgac aagcattttc aattccgcag aatcctttaa aaaagttcta aatcctcatg
    18001 attttggggt ttcagggcaa aatgttgaaa aagaagattt ggacaaactc ttgagccagt
    18061 ttgcaagtga caaaggttat aagattaaag agaaagaagt gcttcgttac acttactttg
    18121 ctgttgcgaa tcaagaagga accaagttaa ctatttttga aaaaggacaa aaccgtgtcc
    18181 aaccaaaaac agttttcatg gtatttgacc aaaaagatta tgaaaatatg actggtcaaa
    18241 aactgtctct atcaggaaat gaggtcggtc tctttgccaa aaatgacgga ctgaaaggac
    18301 agaaagctct aactctaaat gatcatcaat tttctgtcaa agaagaattt aataaagatt
    18361 tcattgtcaa ccatgttcca aataagttta atatcttgac tactgattac aattaccttg
    18421 ttgttcctga tttacaagcc tttttggatc aattcccaga ttcggctatc tataatcagt
    18481 tttacggtgg tatgaatgta gatgccagcg aagaagaaca actcaaggtc gctgaggagt
    18541 atgaaaacta cctcaatcaa tttaatgctc aattagacac agaaggtagc tatgtttatg
    18601 gtagcaatct agcagatgct agttctcaga tgagtgccct ctttggtggt gtcttcttta
    18661 tcggtatttt cctatccatt atctttatgg tcggaactgt tctggtcatc tactacaaac
    18721 aaatttctga aggctacgaa gaccgtgaac gctttattat cttgcagaaa gtcggtttgg
    18781 accaaaagca aatcaagcaa accatcaaca aacaggtttt aactgttttc ttccttcctt
    18841 tgctctttgc cttcatacat ctcgcctttg cctaccatat gcttagcctg attttaaaag
    18901 tgattggtgt actggatacg actatgatgt tgattgtgac cttgtctatc tgcgctatct
    18961 tcctcatcgc ctatgtgctg attttcatga ttacttcaag aagttatcgc aagattgtgc
    19021 aaatgtaaaa aagatacctc gacttcaaaa tcgaggtatt tcttgtattc taaatgctga
    19081 aaagttgttc gagcaggaag gtaactccca tggtcaagag accaatagca aggttccgaa
    19141 tcatagctgt tctagtcgga tcctttccaa gtctggcact agtgtaacca gtgagaagaa
    19201 gggccacacc gacaataagg acagtagcag ggatgcgata ttcacttggg aatatggtca
    19261 ctgacagcat tggaggcaaa cttctaagga aaaaggcaac gaagctagaa atggcagcgt
    19321 gccaaggatt ggtaaattct tcatactcaa tcccatattt ttcctctacc agagccttga
    19381 gtggattttt aagaaagatc ttattggtca agagttgggc agaagttttg cattctccat
    19441 tttggatata agcagcatag agggattttt tggctagttc cctatcttgg tctaacaaga
    19501 gtttttctcg cgaaacggca gcttcctcgg tatcttttgg agttgaaacg gatacatatt
    19561 ctccaccagc cattgaaaag gcaccagcta agatagccgc aaatcctgat aaaaagataa
    19621 tccagatatt ggtcgtggca ctggcaaccc cgataaccac accagcaatg gaaataattc
    19681 catcgttagc accaagaaca cctgcgcgca ggatatttaa acgacctgca aaatttgaat
    19741 cgatttcgtg atttgtttct gacgctaaat ttcaagttca agttagccat caagaagtct
    19801 tctctgggtg acttgtagtt caagcatttt ttaggatagt tgttaatcca cttttcgatg
    19861 aatgcgactt ctttgggagt cattttcttg gttcccttag gtaaccatct atgaatgagc
    19921 ctgttgtgat tctcattagt tcccctttcc caagaggcat aggggtgcgc ataatagatg
    19981 tgctcctcag aaaatacatc aaacaagcga ttgaattccg ttccattatc tgccgtgatg
    20041 gaaagaatct tgtgttgttt taagatgagt tttagagcct gattgaccac ctcagcactt
    20101 ttatttggaa tcaatcggat gatctgatgt ctactctttc gatccgtcaa gacaagcaag
    20161 cagtagtttt tcgctctcgt aagtagaacc gtatcaatct cataatgccc attctccaag
    20221 cgaagattga tagcttcagg ccgctgttcg atggattgac cagcaggttt aaagttggtg
    20281 ctagcctgtt tcttaagcgc ttttcctttt ctagggtaaa gcagatcctg cttgcttaac
    20341 cccaattttc cacgatgaat ccaatagtaa atggttgaaa ttcccacgtt aaccccttta
    20401 gcaataacca tcatttcagg cgaaaatttt tggttatgat agtggagaat cttttccttt
    20461 agttccttgg tcaagcttga tttcttgacc gagcgcttgc gattgttttc ataagactgt
    20521 tgagcatagt cggcagaata aacctctttg aagcgccctt ttccaagaca ttgtcggact
    20581 gtcccacgct tgatttcagt gtggatagtt tgaggagctt ttccaagtag agaggcaatt
    20641 tctctatttg atttcccttc ttttttccat ctttcgatta agcgacggct atcgattgtc
    20701 aaatgtttgg cttttgtagt ataattgtct tgcatctctg tgcctttctt gtgtttgtgg
    20761 ttgaacaaca agtataacac agaggtgttt tcttatgcct acaagagcta tcggctagtt
    20821 gaaccatcta atttttagga gggctgggtg gctaacttca ttatagaact ttcatttacg
    20881 aacatatagt aaaatgaaac aagaacagaa caaatcgatc aggacagtaa aatctatttc
    20941 taacaatgtt ttagaagcag aggtgtacta ttcta
//
LOCUS       DAVKQL010000038        20836 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVKQL010000038 DAVKQL010000000
VERSION     DAVKQL010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20836
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.038"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     misc_feature    80..211
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            227..757
                     /gene="infC"
                     /locus_tag="VW561_001351"
     CDS             227..757
                     /gene="infC"
                     /locus_tag="VW561_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU9943602.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            790..990
                     /gene="rpmI"
                     /locus_tag="VW561_001352"
     CDS             790..990
                     /gene="rpmI"
                     /locus_tag="VW561_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU9943603.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            1042..1401
                     /gene="rplT"
                     /locus_tag="VW561_001353"
     CDS             1042..1401
                     /gene="rplT"
                     /locus_tag="VW561_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEU9943604.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            1459..1839
                     /locus_tag="VW561_001354"
     CDS             1459..1839
                     /locus_tag="VW561_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9943605.1"
                     /translation="MVSKMLHTCLRVENLEKSIAFYKDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            2089..2889
                     /locus_tag="VW561_001355"
     CDS             2089..2889
                     /locus_tag="VW561_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEU9943606.1"
                     /translation="MNLTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            2852..3838
                     /locus_tag="VW561_001356"
     CDS             2852..3838
                     /locus_tag="VW561_001356"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEU9943607.1"
                     /translation="MVLFSAQEQLYYKEKIMTTNRLQVSLPGLDLKNPIIPASGCFGF
                     GQEYAKYYDLNLLGSIMIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAE
                     KLPWLEREYPNLPIIANVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGL
                     LIGQDPDLAYDVVKAAVEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVG
                     MRFDLKTRKPILANGTGGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEM
                     YLAGASAIGVGTANFTNPYACPDIIENLPKVMDKYGISSLEELRQEVKEFLR"
     gene            3880..5598
                     /locus_tag="VW561_001357"
     CDS             3880..5598
                     /locus_tag="VW561_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEU9943608.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGTMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYMTANEWIWDKESWFYLKSDGKIAEKEWVYDSHSQ
                     AWYYFKSGGYMTANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYMAKN
                     ETVDGYQLGSDGKWLGGKATNKNAAYYQVVPVTANVYDSDGEKLSYISQGSVVWLDKD
                     RKSDDKRLAITISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIPVAS
                     HLSDMEVGKKYYSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINNSLL
                     ENKGATFKEAEEHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYLSAK
                     TFDDVDKGILGATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMKINE
                     KLGGKD"
     gene            complement(5659..7314)
                     /gene="pavA"
                     /locus_tag="VW561_001358"
     CDS             complement(5659..7314)
                     /gene="pavA"
                     /locus_tag="VW561_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEU9943609.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKIIEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTKTSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            7419..7916
                     /gene="ybeY"
                     /locus_tag="VW561_001359"
     CDS             7419..7916
                     /gene="ybeY"
                     /locus_tag="VW561_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU9943610.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            7897..8292
                     /locus_tag="VW561_001360"
     CDS             7897..8292
                     /locus_tag="VW561_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEU9943611.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFALTGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLNIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            8309..9208
                     /gene="era"
                     /locus_tag="VW561_001361"
     CDS             8309..9208
                     /gene="era"
                     /locus_tag="VW561_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEU9943612.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            9257..10081
                     /gene="mutM"
                     /locus_tag="VW561_001362"
     CDS             9257..10081
                     /gene="mutM"
                     /locus_tag="VW561_001362"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEU9943613.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQAALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            10081..10686
                     /locus_tag="VW561_001363"
     CDS             10081..10686
                     /locus_tag="VW561_001363"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU9943614.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKPGG
                     RLFEALVQHFWQEIILENGELNRPLLASLIFSNPDEREWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            10673..11872
                     /locus_tag="VW561_001364"
     CDS             10673..11872
                     /locus_tag="VW561_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEU9943615.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSEQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            11872..12021
                     /gene="rpmG"
                     /locus_tag="VW561_001365"
     CDS             11872..12021
                     /gene="rpmG"
                     /locus_tag="VW561_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9943616.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHTDKIEVLKYCPKERKVTL
                     HLESK"
     gene            12061..12294
                     /gene="secG"
                     /locus_tag="VW561_001366"
     CDS             12061..12294
                     /gene="secG"
                     /locus_tag="VW561_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU9943617.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            12396..14750
                     /gene="rnr"
                     /locus_tag="VW561_001367"
     CDS             12396..14750
                     /gene="rnr"
                     /locus_tag="VW561_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEU9943618.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEAKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLELRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKKYHK
                     IVPSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGESYADVLSMMLLRSMQQARYSEHNYGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            14713..15180
                     /gene="smpB"
                     /locus_tag="VW561_001368"
     CDS             14713..15180
                     /gene="smpB"
                     /locus_tag="VW561_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEU9943619.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKRQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            15196..16056
                     /gene="tehB"
                     /locus_tag="VW561_001369"
     CDS             15196..16056
                     /gene="tehB"
                     /locus_tag="VW561_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEU9943620.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMETVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            16129..17082
                     /gene="coiA"
                     /locus_tag="VW561_001370"
     CDS             16129..17082
                     /gene="coiA"
                     /locus_tag="VW561_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEU9943621.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            17101..18903
                     /gene="pepF"
                     /locus_tag="VW561_001371"
     CDS             17101..18903
                     /gene="pepF"
                     /locus_tag="VW561_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU9943622.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQMEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYDPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLKGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALVVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHLYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            18905..19618
                     /locus_tag="VW561_001372"
     CDS             18905..19618
                     /locus_tag="VW561_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEU9943623.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGSLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            19685..20626
                     /gene="prsA"
                     /locus_tag="VW561_001373"
     CDS             19685..20626
                     /gene="prsA"
                     /locus_tag="VW561_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEU9943624.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIVG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(20745..>20836)
                     /locus_tag="VW561_001374"
     CDS             complement(20745..>20836)
                     /locus_tag="VW561_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU9943625.1"
                     /translation="DYIDYYNNKRIKIKLKGLSSVQYRTKSFG"
BASE COUNT         6324 a         3862 c         4693 g         5957 t
ORIGIN      
        1 caaaatacaa acacaaactt ttcaaaaaaa taacttttta tcttgacaag agctagaaaa
       61 cttggtatca tttaaaagtt gagaaaagca gaagtgagag cttctcgcct tgtgacatta
      121 agttgcctgg ccctacggat gaaaagtttc gaagaaacgc tatcataacg tgcgggcttg
      181 tatatttacg agtccgctat tgtttttctc taataaaaca aaagaggtga aaaccatagc
      241 aaagcaagac ttattcatca atgatgagat tcgtgtacgt gaagttcgct tgattggtct
      301 tgagggagaa cagctaggca tcaagccact cagtgaagcg caagctttgg ctgataacgc
      361 taatgttgac ctagtattga ttcaacccca agccaaaccg cctgttgcaa aaattatgga
      421 ctacggtaag ttcaaatttg agtaccagaa gaagcaaaaa gaacaacgta aaaaacaaag
      481 cgttgttact gtgaaagaag ttcgtctaag tccaactatt gacaagggtg actttgatac
      541 aaaacttcgt aatgcacgta agttccttga aaaaggaaat aaagttaagg tatctattcg
      601 ctttaagggt cgtatgatta cccataaaga gattggtgca aaagttttag ccgagtttgc
      661 tgaagcaact caagatattg ccatcatcga acaacgtgct aaaatggatg gacgtcaaat
      721 gttcatgcaa ttggcgccag cgactgacaa aaaataattg tcagaaagtt aaataaagga
      781 gaaaaaatca tgccaaaaca aaaaacacac cgcgcatcag ctaaacgttt caaacgtaca
      841 ggttctggtg gacttaaacg tttccgtgct tacacttctc accgtttcca cggaaaaact
      901 aagaaacaac gtcgtcatct tcgtaaagca tctatggtgc attcaggaga ttacaaacgt
      961 atcaaagcaa tgcttactcg cttgaaataa tagcttgact gtaagtaaac aattctagga
     1021 aatatttgga ggaaataaaa catggcacgt gttaaaggtg gcgttgtatc acgcaaacgt
     1081 cgtaaacgta ttcttaaatt agcaaaaggt tactatggag ctaaacacat cttgttccgt
     1141 actgcaaaag aacaagtaat gaactcttac tactatgcat accgtgaccg tcgtcagaaa
     1201 aaacgtgact tccgcaaatt gtggattact cgtatcaatg cggcagctcg tatgaacgga
     1261 ctttcatact cacaattgat gcatggtttg aaattggctg agattgaagt taaccgtaaa
     1321 atgcttgctg acttggctgt taacgatgca gtagctttca cagctcttgc agatgcagct
     1381 aaagcaaaac ttggtaaata acaactgatg agactggaac aggattgtcc agtcttttta
     1441 aaaataaagg agaaagatat ggtttcaaaa atgctacaca cttgcttacg agtagaaaat
     1501 cttgaaaaat caatcgcatt ttataaagat gcttttggtt ttaaagaatt gcgtcgcaga
     1561 gattttccag accatgcctt cacgattgtc tatctaggtc ttgagggtga tgattatgag
     1621 ttggagttga cttataacta cgatcacggt ccgtatgtgg ttggtgatgg gtttgcccat
     1681 atcgccctca gtacacctga tcttgaggca cttcatcaag aacacagtgc aaaaggctat
     1741 gaagtgactg agccaaatgg tctaccagga actacaccaa actattactt tgttaaagac
     1801 cctgatggat ataaggtcga agtcattcgt gaaaaataat ttcgtgcaag ttaagtagaa
     1861 tattcgtttt ctacttgact ttttttagct gaaagagtat actaataaaa attcaacctt
     1921 taaggggagt ccagagagac tcacaaggtg tcagataaaa gaatagtgca attttctaga
     1981 ggagaccttt tgagtgtgct ctcttgtgtt gtacgatttt aactgaggcc ttgcactagc
     2041 aaggtctttt ctttatctgg tccccttaaa atttaaggag gaaaagttat gaatctcaca
     2101 tgtaagaagc gtttgggtgt cattcggttg gaaaccatga aggtggttgc acaagaggaa
     2161 atcgcgccag caatctttga attagtccta gaaggagaaa tggttgaagc catgcgagca
     2221 ggccaatttc ttcatctgcg tgtaccagac gatgcccatc tcttacgtcg tcctatttca
     2281 atttcgtcta ttgacaaggc aaacaagcag tgtcacctca tttatcggat tgagggagct
     2341 gggactgcaa ttttttcaac cttaagtcag ggagacactc ttgatgtgat ggggcctcag
     2401 ggaaatggtt ttgacttgtc tgaccttgat gagcagaatc aggttctcct tgttggtggt
     2461 gggattggtg ttccaccctt gcttgaggtg gccaaggaat tgcatgaacg tggagtgaaa
     2521 gtagtgacag tcctcggttt tgctaataag gatgctgtta ttttgaaaac ggaattggct
     2581 cagtatggtc aggtctttgt aacgacagat gatggttctt atggcatcaa gggaaatgtt
     2641 tccgttgtta tcaatgattt agatagtcag tttgatgctg tttactcgtg tggggctcca
     2701 ggaatgatga agtatatcaa tcaaaccttt gatgatcacc caagagccta tttatctctg
     2761 gaatctcgta tggcttgtgg gatgggagct tgctatgcct gtgttctaaa agtaccagaa
     2821 agcgagacgg tcagccaacg cgtctgtgaa gatggtcctg ttttccgcac aggaacagtt
     2881 gtattataag gagaaaatta tgactacaaa tcgattacaa gtgtctctac ctggtttgga
     2941 tttgaaaaat ccgattattc cagcatcagg ctgttttggc tttggacaag agtatgccaa
     3001 gtactatgat ttaaaccttt taggttctat tatgatcaag gcgacaaccc ttgaaccacg
     3061 ttttgggaac ccaactccaa gagtagcaga gacgcctgct ggtatgctca atgcaattgg
     3121 cttgcaaaat cctggtttag aggttgtttt ggctgaaaag ctaccttggc tggaaagaga
     3181 atatccaaat cttcctatta ttgccaatgt agctggtttt tcaaaacaag agtatgcagc
     3241 tgtttctcat gggatttcca aggcaactaa tgtaaaagct atcgagctca atatttcttg
     3301 tcccaatgtt gaccactgta atcatggact tttgattggt caagatccag atttggctta
     3361 tgatgtggtg aaagcagctg tggaagcctc agaagtgcca gtttatgtca aattaacccc
     3421 gagtgtgacc gatatcgtta ctgtcgcaaa agctgcagaa gatgcgggag caagtggctt
     3481 gaccatgatc aatactctgg ttggaatgcg ctttgacctc aaaaccagaa aaccaatctt
     3541 ggccaatgga acaggtggaa tgtcaggtcc agcagtcttt ccagtagccc tcaaactcat
     3601 ccgccaagta gcccaaacaa cagacctgcc tatcattgga atggggggag tggattcggc
     3661 tgaagctgcc ctagaaatgt atctggctgg ggcatctgct atcggagttg gaacagctaa
     3721 ctttaccaat ccttatgcct gccctgacat catcgaaaat ttaccaaaag tcatggataa
     3781 atacggtatt agcagtctgg aagaactccg tcaggaagta aaagagtttc tgaggtaaac
     3841 tgcaatcaat ctgttcttga ttttttatta gtttgtaata tgaatttagg agaattttgg
     3901 tacaataaaa taaataagaa cagaggaaga aggttaatga agaaagtaag atttattttt
     3961 ttagctctgc tatttttctt agctagtcca gaaggtacaa tggctagtga tggtacttgg
     4021 caaggaaaac agtatctgaa agaagatggc agtcaagcag cgaatgagtg ggtttttgat
     4081 actcattatc aatcttggtt ctatataaaa gcagatgcta actatgctga aaatgaatgg
     4141 ctaaagcaag gtgacgacta tttttacctc aaatctggtg gctatatggc caaatcagaa
     4201 tgggtagaag acaagggagc cttttattat cttgaccaag atggaaagat gaaaagaaat
     4261 gcttgggtag gaacttccta tgttggtgca acaggtgcca aagtaataga agactgggtc
     4321 tatgattctc aatacgatgc ttggttttat atcaaagcag atggacagca cgcagagaaa
     4381 gaatggctcc aaattaaagg gaaggactat tatttcaaat ccggtggtta catgacagcc
     4441 aatgaatgga tttgggataa ggaatcttgg ttttatctca aatctgatgg gaaaatagct
     4501 gaaaaagaat gggtctacga ttctcatagt caagcttggt actacttcaa atccggtggt
     4561 tacatgacag ccaatgaatg gatttgggat aaggaatctt ggttttacct caagtctgat
     4621 ggcaaaatgg ctgaaaaaga atgggtctac gattctcata gtcaagcctg gtactacttc
     4681 aaatctggtg gctacatggc gaaaaatgag acagtagatg gttatcagct tggaagcgat
     4741 ggtaaatggc ttggaggaaa agctacaaat aaaaatgctg cttactatca agtagtgcct
     4801 gtcacagcca atgtttatga ttcagatggt gaaaagcttt cctatatatc gcaaggtagt
     4861 gtcgtatggc tagataagga tagaaaaagt gatgacaagc gcttggctat tactatttct
     4921 ggtttgtcag gctatatgaa aacagaagat ttacaagcgc tagatgctag taaggacttt
     4981 atcccttatt atgagagtga tggccaccgt ttttatcact atgtggctca gaatgctagt
     5041 atcccagtag cttctcatct ttctgatatg gaagtaggca agaaatatta ttcggcagat
     5101 ggcctgcatt ttgatggttt taagcttgag aatcccttcc ttttcaaaga tttaacagag
     5161 gctacaaact acagtgctga agaattggat aaggtattta gtttgctaaa cattaacaat
     5221 agccttttgg agaacaaggg cgctactttt aaggaagccg aagaacatta ccatatcaat
     5281 gccctttatc tccttgccca tagtgcccta gaaagtaact ggggaagaag taaaattgcc
     5341 aaagataaga ataatttctt tggcattaca gcctatgata cgacccctta cctttctgcc
     5401 aagacatttg atgatgtgga taagggaatt ttaggtgcaa ccaagtggat taaggaaaat
     5461 tatatcgata ggggaagaac tttccttgga aacaaggctt ctggtatgaa tgtggaatat
     5521 gcttcagacc cttattgggg cgaaaaaatt gctagtgtga tgatgaaaat caatgagaag
     5581 ctaggtggca aagattagta ctataagtga atatgatttg agtgaatagt aagttaaaaa
     5641 tcctgatttc aagtaaaatc aggatttttt catggatgca atttttttgg agtctggtgt
     5701 gacgcggagg gtcttttgtc ctgtgtaagt gacaaagccg ggttttccac cagttggttt
     5761 attgagtttt ttgacttcaa tcatatctac ctgcaccaga ttcgacaggc gcccttgaga
     5821 gaagtaggca gctaactctg ctgcgtctgt cttgactgca tcagatgggt caagatttcc
     5881 tgagatgaca acatggcttc caggaatgtc cttagcatgg aaccaaagtt cctccttgcg
     5941 ggccatttta aaggtcaatt cctcattttg aagattgttt cgtccgacat agatgatggt
     6001 tttgccatcg cttgctagat attgttctag ttttttgcgt ttctggattt tctcccgttg
     6061 tcttctgcgg ataaaacctg tttgaatcaa ttcttcacgg atttcagcga tttcttccag
     6121 tccagcttgg ttgaggacgg tttctacact ttccagatag agaatagtgg ctttggtttc
     6181 ttcaatcaaa tcagtcaagt atttgacagc ttctttgagt ttctgatacc gtttaaaata
     6241 gcgttgggca ttctggttgg gagtcagagc cttatcaagc gcaatcatga taggttggtt
     6301 ggtatagtag ttgtctagga taacctggtc ttggtcgtta ggcacttggt gtaggaaggt
     6361 tgtcagcaat tctccttttt gacgaaattc ttcagcgttg tctgtcgcca gtaactcttt
     6421 ttcctgtttt ttgagtttgt gtcggttttt ctgaagttca ttttcaacac gacgaatcag
     6481 ttcactggcc tgctgtttga cgcggtcgcg ctcagcctta tccttatagt aggtgtccaa
     6541 caaatcagaa agatttgcaa aaggctctcc cacctgattt gcaaaaggaa ctggactgaa
     6601 ggaagtctta gtcaagcatg gcttggtttc ttgattgaaa aaatttcgga aagcggaaag
     6661 tttttcacta accagtatcc tttccaattc atttgccgta tcgcgtccca gaccttgaaa
     6721 gaggctttga agattttttg ctgttagttc ttgggtttgc aggatttcaa agagcttttc
     6781 atccttgata gtaaaaggat tgagagattc tgtacttggc ggagcgatat aggtcgatcc
     6841 tggaagtaag gtgcggtagc tattttgtga aaaaccgacg tgtttgataa cttcgatgat
     6901 tttatgactg cttttatcga ccagtagaat attactgtgt ttccccataa tttcaataat
     6961 caaggtagcc tggatatggt ctccaatctc gtttttattg gaaactgtaa tttccacaat
     7021 acggtcattt tccacttgct caatcgactc aatcagggcc ccctgcaaat actttctcaa
     7081 aaccatgata aaggtagaag gttgagctgg attttcaaaa gtcgtttggg tcagctgaat
     7141 gcgtccaaaa actggatggg cagaaaggag caggcgatgg ctttggcgat tgctgcggat
     7201 ttgcaagacc aactcttgtt caaaaggctg attgattttc tggatgcgac cattcactaa
     7261 ttcgcttcgc aattcctcaa ctatgtggtg taaaaaaaat ccatcaaatg acatcgttct
     7321 ctccttgtga ttgtattcca tagtattata tcaaaaaggt agaataaaat catagaaatg
     7381 tggtataata aagccaagta aagagaaacg agaagcacat gtatattgaa atggtagatg
     7441 aaactggtca agtttcaaaa gaaatgttgc aacaaaccca agaaattttg gaatttgcag
     7501 cccaaaaatt aggaaaagaa gacaaggaga tggcagtcac ttttgtgacc aatgagcgta
     7561 gtcatgaact taatctggag taccgtgaca ccgaccgtcc gacagatgtc atcagccttg
     7621 agtataaacc agaattggaa attgcctttg acgaagagga tttgcttgaa aatccagaat
     7681 tggcagagat gatgtctgag tttgatgcct atattgggga attgttcatc tctatcgata
     7741 aggctcatga gcaggctgaa gaatatggtc acagctttga gcgtgagatg ggcttcttgg
     7801 cagtacacgg ctttttacat attaacggct atgatcacta cactccggaa gaagaagcgg
     7861 agatgttcgg tttacaagaa gaaattttga cagcctatgg actcacaaga caataaacga
     7921 aaatggaaaa atcgtgactt gatatccagt ttagaatttg ctttgacagg tatttttact
     7981 gctatcaagg aagaacgcaa tatgcgaaaa cacgcagtga cggctctagt ggtcatcctt
     8041 gcaggttttg tttttcaggt gtcacgaatc gaatggctct ttctcctatt gaatattttc
     8101 ttggtagtag cctttgagat tatcaactct gctattgaaa atgtggtgga tttggccagt
     8161 cactatcact tttccatgct ggctaaaaat gccaaggata tggcggccgg cgcggtatta
     8221 gtggtttctc ttttcgcagc cttaacaggc gcattgattt ttctcccacg aatctgggat
     8281 ttattatttt aaacagtaag aggaaattat gacttttaaa tcaggctttg tagccatttt
     8341 aggacgtccc aatgttggga agtcaacctt tttaaatcac gttatggggc aaaagattgc
     8401 catcatgagt gacaaggcgc agacaacgcg caataaaatc atgggaattt acacgactga
     8461 taaggagcaa attgtcttta tcgacacacc agggattcac aagcctaaaa cagctctcgg
     8521 agatttcatg gttgagtctg cctacagtac ccttcgcgaa gtggacaccg ttcttttcat
     8581 ggtgcctgct gatgaagcgc gtggtaaggg ggacgatatg attatcgagc gtctcaaggc
     8641 tgccaaggtt cctgtgattt tggtggtgaa taaaatcgat aaggtccatc cagaccagct
     8701 cttgtctcag attgatgact tccgtaatca aatggacttt aaggaaattg ttccaatctc
     8761 agcccttcag ggaaataacg tgtctcgtct agtggatatt ttgagtgaaa atctggatga
     8821 aggtttccaa tatttcccgt ctgatcaaat cacagaccat ccagaacgtt tcttggtttc
     8881 agaaatggtt cgcgagaaag tcttgcacct aactcgtgaa gagattccgc attctgtagc
     8941 agtagttgtt gactctatga aacgagacga agagacagac aaggttcaca tccgtgcaac
     9001 catcatggtc gagcgcgata gccaaaaagg gattatcatc ggtaaaggtg gcgctatgct
     9061 taagaaaatc ggtagcatgg cccgtcgtga tatcgaactc atgctaggag acaaggtctt
     9121 cctagaaacc tgggtcaagg tcaagaaaaa ctggcgcgat aaaaagctag atttggctga
     9181 ctttggctat aatgaaagag aatactaagt agaggtaggc tcatgcctgc ttcttgtttt
     9241 tacagaagga ggacttatgc ctgaattacc tgaggttgaa accgtttgtc gtggcttaga
     9301 aaaattgatt ataggaaaga agatttcgag tatagaaatt cgctacccca agatgattaa
     9361 gacggatttg gaagagtttc aaagggaatt gcctagtcag attatcgagt caatgggacg
     9421 tcgtggaaaa tatttgcttt tttatctgac agacaaggtc ttgatttccc atttgcggat
     9481 ggagggcaag tatttttact atccagacca aggacctgaa cgcaagcatg cccatgtttt
     9541 ctttcatttt gaagatggtg gcacgcttgt ttatgaggat gttcgcaagt ttggaaccat
     9601 ggaactcttg gtgcctgacc ttttagacgc ctactttatt tctaaaaaat taggtcctga
     9661 accaagcgaa caagactttg atttacaggt ctttcaagct gcccttgcca agtccaaaaa
     9721 gcctatcaaa tcccatctcc tagaccagac cttggtagct ggacttggca atatctatgt
     9781 ggatgaggtt ctctggcgag ctcaggttca tccagctaga ccttcccaga ctttgacagc
     9841 agaagaagcg actgccattc atgaccagac cattgctgtt ttgggccagg ctgttgaaaa
     9901 aggtggctcc accattcgga cttataccaa tgcctttggg gaagatggaa gcatgcagga
     9961 ctttcatcag gtctatgata agactggtca agaatgtgta cgctgtggta ccatcattga
    10021 gaaaattcaa ctaggcggac gtggaaccca cttttgtcca aactgtcaaa ggagggactg
    10081 atgggaaaaa tcatcggaat cactggggga attgcctctg gtaagtcaac tgtgacaaat
    10141 tttctaagac agcaaggctt tcaagtagtg gatgccgacg cagtcgtcca ccaactacag
    10201 aaacctggtg gtcgtctgtt tgaggctcta gtacagcact tttggcaaga aatcattctt
    10261 gaaaacggag aactcaatcg ccctctccta gctagtctca tcttttcaaa tcctgatgaa
    10321 cgagaatggt ctaagcaaat tcaaggggag attatccgtg aggaactggc tactttgaga
    10381 gaacagttgg ctcagacaga agagattttc ttcatggata ttcccctact ttttgagcag
    10441 gactacagcg attggtttgc tgagacttgg ttggtctatg tggaccgaga tgcccaagta
    10501 gaacgcttaa tgaaaaggga ccagttgtcc aaagatgaag ctgagtctcg tctggcagcc
    10561 cagtggcctt tagaaaaaaa gaaagatttg gccagccagg ttcttgataa taatggcaat
    10621 cagaaccagc ttcttaatca agtgcatatc cttcttgagg gaggtaggca agatgacaga
    10681 gattaactgg aaggataatc tgcgcattgc ctggtttggt aattttctga caggagccag
    10741 tatttctttg gttgtacctt ttatgcccat cttcgtggaa aatctaggtg tagggagtga
    10801 gcaagtcgct ttttatgcag gcttagcaat ttctgtctct gctatttccg cggcgctctt
    10861 ttctcctatt tggggtattc ttgctgacaa atacggccga aaacccatga tgattcgggc
    10921 aggtcttgct atgactatca ctatgggagg cttggccttt gtcccaaata tctattggtt
    10981 aatctttctt cgtttactaa acggtgtatt tgcaggtttt gttcctaatg caacggcact
    11041 gatagccagt caggttccaa aggagaaatc aggctctgcc ttaggtactt tgtctacagg
    11101 cgtagttgca ggtactctaa ctggtccctt tattggtggc tttatcgcag aattatttgg
    11161 cattcgtaca gttttcttac tggttggtag ttttctattt ttagctgcta ttttgactat
    11221 ttgctttatc aaggaagatt ttcaaccagt agccaaggaa aaggctattc caacaaagga
    11281 attatttacc tcggttaaat atccctatct tttgctcaat ctctttttaa ccagttttgt
    11341 catccaattt tcagctcaat cgattggccc tattttggct ctttatgtac gcgacttagg
    11401 gcagacagag aatcttcttt ttgtctctgg tttgattgtg tccagtatgg gcttttccag
    11461 catgatgagt gcaggagtca tgggcaagct aggtgacaag gtgggcaatc atcgtctctt
    11521 ggttgtcgcc cagttttatt cagtcatcat ctatctcctc tgtgccaatg cctctagccc
    11581 ccttcaacta ggactctatc gtttcctctt tggattggga accggtgcct tgattcccgg
    11641 ggttaatgcc ctactcagca aaatgactcc caaagccggc atttcgaggg tctttgcctt
    11701 caatcaggta ttcttttatc tgggaggtgt tgttggtccc atggcaggtt ctgcagtagc
    11761 aggtcaattt ggctaccatg ctgtctttta tgcgacgagc ctttgtgttg cctttagttg
    11821 tctctttaac ctgattcaat ttcgaacatt attaaaagta aaggaaatct agtgcgagta
    11881 aaaattaatc tcaaatgctc ctcttgtggc agtatcaatt acctaaccag taaaaattca
    11941 aaaacccata cagacaagat tgaggtttta aagtattgtc ccaaggaaag aaaagtaacc
    12001 ctacatcttg aatctaagta gattttatgg taaaataata gggattttaa ggagtttgat
    12061 atgtataacc tattattaac cattttatta gtattatctg ttgtgattgt gattgcaatt
    12121 ttcatgcaac caaccaaaaa ccaatccagc aatgtatttg atgccagttc aggtgatttg
    12181 tttgaacgca gtaaagctcg cggttttgaa gctgtaatgc agcgtttgac agggatttta
    12241 gtctttttct ggctagccat tgccttagca ttgacggtat tatcaagtag ataagaaaat
    12301 aatgggcagg actaggtctt tgcctctttt tatttttaaa ggatgtttga gaaggtttta
    12361 cagtaaaaga aaattaaaaa atctagaaag aaaatatgaa agatagaata aaagaatatt
    12421 tacaagacaa gggaaaggtg actgttaatg atttggctca ggctttggga aaagacagtt
    12481 ccaaggattt tcgtgagttg attaaaacct tgtccttaat ggaaagaaag caccagattc
    12541 gctttgaaga agatggcagt ctgaccttgg aagccaagaa aaaacatgag attaccctca
    12601 aggggatttt tcatgcccat aaaaatggtt ttggttttgt cagcctagaa ggggaagagg
    12661 acgacctttt tgtagggaaa aatgatgtca actatgctat tgatggtgat accgtcgagg
    12721 tagtgattaa gaaagtcgct gaccgcaata agggaacagc agcagaagcc aaaattattg
    12781 atatcctaga acacagtttg acaacagttg tcgggcaaat cgttctggat caggaaaagc
    12841 ctaagtatgc tggctatatt cgttcaaaaa atcagaaaat cagtcaaccg atttatgtta
    12901 agaaaccagc cctaaagtta gaaggaacag aagttctcaa ggtctttatc gataaatacc
    12961 caagcaagaa acatgatttc tttgtcgcga gtgttctcga tgtggtggga cactcaacgg
    13021 atgtcggaat tgatgttctt gaggttttgg aatcaatgga cattgtatcc gagtttccag
    13081 aagctgttgt taaggaagca gaaagtgtgc ctgatgctcc gtctcaaaag gatatggaag
    13141 gtcgtctgga actaagagat gaaattacct ttaccattga cggtgcggat gccaaggact
    13201 tggacgatgc agtgcatatc aaggctctga aaaatggcaa tctggagctt ggggttcaca
    13261 tcgcagatgt ttcttattat gtgaccgagg ggtctgccct tgacaaggaa gcccttaacc
    13321 gtgcgacttc tgtttacgtg acagaccgag tggtgccaat gcttccagaa cgactatcaa
    13381 atggcatctg ctctctcaat ccccaagttg accgcctgac ccagtctgct attatggaga
    13441 ttgataaaca tggtcgtgtg gtcaactata ccattacaca aacagttatc aagaccagtt
    13501 ttcgtatgac ctatagcgat gtcaatgata tcctagctgg cgatgaagaa aagagaaaaa
    13561 aatatcataa aattgtacca agtatcgaac tcatggccaa gcttcatgaa actttagaaa
    13621 acatgcgtgt gaaacgtgga gctctcaatt ttgataccaa tgaagcgaag attttagtgg
    13681 ataaacaagg taagcctgtt gatatcgttc ttcggcagcg tggtattgcc gagcggatga
    13741 ttgagtcttt tatgttgatg gctaatgaaa cagttgccga acatttcagc aagttggatt
    13801 tgccttttat ctatcgaatt cacgaggagc ctaaggctga aaaggttcag aagtttattg
    13861 attatgcttc gagttttggc ttgcgcattt atgggactgc cagtgagatt agtcaggagg
    13921 cacttcaaga catcatgcgt gctgttgagg gagaatctta tgcagatgta ttgtccatga
    13981 tgcttcttcg ctctatgcag caggctcgtt attcggagca caattacggc cactatggac
    14041 tagctgctga ctattatact cactttacca gtccaattcg tcgttatcca gaccttcttg
    14101 ttcaccgtat gattcgggat tacggccgtt ctaaggaaat agcagagcat tttgaacaag
    14161 tgattccaga gattgcgacc cagtcttcca accgtgaacg tcgtgccata gaagctgagc
    14221 gtgaagtcga agccatgaaa aaggctgagt atatggaaga atacgtgggt gaagagtatg
    14281 atgcagttgt atcaagtatt gtcaaattcg gtctctttgt cgaattgcca aacacagttg
    14341 aaggcttgat tcacatcact aatctacctg aattttatca tttcaatgag cgtgatttga
    14401 ctcttcgtgg agaaaaatca ggtatcactt tccgagtggg tcagcagatc cgtatccgtg
    14461 ttgaaagagc ggataaaatg actggagaga ttgatttttc attcgtacct agtgagtttg
    14521 atgtgattga aaaaggcttg aaacagtcta gtcgtagtgg cagagggcgt ggttcaaatc
    14581 gtcgttcgga taagaaggaa gacaagagaa aatcaggacg ctcaaatgat aagcgtaagc
    14641 attcacaaaa agacaagaag aaaaaaggaa agaaaccttt ttacaaggaa gtagctaaga
    14701 aaggagccaa gcatggcaaa gggcgaggga aaggtcgtcg cacaaaataa aaaggcacgc
    14761 cacgactata caatcgtaga tacgctagag gcagggatgg tcctgactgg aactgaaatc
    14821 aagagtgtac gagctgctcg aattaatctc aaggatggct ttgctcaagt gaaaaatgga
    14881 gaagtttggc tgagcaatgt tcatatcgcg ccttacgaag agggcaatat ctggaaccag
    14941 gaaccagaac gtcgtcgtaa actcctgctc cataaaaggc aaattcaaaa attggaacaa
    15001 gagaccaaag ggacaggaat gaccttagtt ccccttaagg tctatataaa agatggctac
    15061 gctaagcttc ttttaggact tgccaaaggg aagcatgact atgacaaacg ggagtctatc
    15121 aaacgtcgtg agcaaaatcg agatatcgcg cgtgtgatga aagctgttaa tcagcgataa
    15181 aaagaggaat tgaaaatgga aaaattagtt gcctataaac gcatgccttt gtggaataaa
    15241 caaacaatgc ctgaagctgt tcagcaaaag cacaatacaa aagttgggac ttgggggaaa
    15301 attactgtct tgaagggagc tctcaagttt attgaattga cagaagaagg ggaagttcta
    15361 gctgaacacc tctttgaagc aggggcagac aatccaatgg cccaacctca agcctggcac
    15421 cgagtggaag ctgccacaga tgatgtggaa tggtacttgg aattttattg taaacctgag
    15481 gattattttg ctaaaaaata caataccaat cctgttcatt cagaggtcct agaggccatg
    15541 gagacagtga aacaagggaa agctttggat ttgggttgtg gtcaggggcg taattctctt
    15601 tttctagccc agcaagattt tgatgtgacg gctgtagatc aaaatggact agctcttgaa
    15661 atcttgcaaa gcattgtgga gcaggaagat ttggacatgc ctgttggcct ttacgatatc
    15721 aattcagcta gcattgaaca agaatatgat tttatcgttt caacagttgt tctcatgttt
    15781 ctacaagcgg accgcattcc agctattatt caaaatatgc aggagaaaac cagtgttggt
    15841 ggttacaacc ttatcgtttg tgccatggac acggaggatt atccttgctc ggttaacttc
    15901 ccattcacct ttaaagaagg agaactggca gactattaca aggattggga attggttaag
    15961 tacaatgaaa atccaggcca tttgcatcgt cgcgatgaga atggcaatcg tattcaacta
    16021 cgctttgcga ccttactagc taagaaaatc aagtaaacac acatgaagat taggaatttt
    16081 cctgatcttt tttctttttt acgaatgata tagaaaagga gggaattcat gtttgttgcg
    16141 agagatgcta ggggagaatt ggtaaatgtg ttagaggata aacttgagaa gcaagcatac
    16201 acctgcccag cttgtggagg ccagctccat ttgcgtcaag gaccaagtgt acggacgcat
    16261 tttgcccata aatccttaaa agactgtgat tttttctttg aaaatgaaag tccagaacac
    16321 ctggccaata aggaatccct ctatcactgg ttgaaaaaag agacaaaggt tcaattagag
    16381 tacccgcttt cagaacttaa acagattgcg gatgtatttg taaatggcaa tctagctcta
    16441 gaagttcagt gtagtccctt gcctcagaaa gtccttaaag agcgaagtga gggctatcgt
    16501 agtcagggtt accaagtact gtggttgctg ggtcaaaaac tgtggctcaa ggagcgtttg
    16561 actcgtctac agcaaggttt tctttatttc agtcaaaaca tgggctttta tgtttgggaa
    16621 ttagacaagg aaaaacaagt tttaagactc aaatacctga tttaccagga tctccgcggt
    16681 aaactccatt atcaaatcaa ggaattttcc tatggtcaag gtagtttatt ggaaatattg
    16741 cgtcttccct ataagagaca aaaaatatct cattttacag tttctgagga caaggacatc
    16801 tgtcgctata tccggcaaca actttattat caaaatctct tttggatgaa agaacaagca
    16861 gaagcctatc aaaagggaga aaatatcctg acttatggac tgaaagaatg gtatccacaa
    16921 attcgaccaa tagtgggcaa atttttccag attgaacaag acttgactag ctattatcag
    16981 cacttttata cctattacca aaaaaatcct caaaatgatt ggcaaaagct ttatccacca
    17041 gccttttatc agcaatattt cttgaaaaat atggtagaat agaaaggatg gaggaatcta
    17101 atggtattac aaagaaatga aataaatgaa aaagatacat gggatctatc aacgatctac
    17161 ccaactgacc aggcttggga agaagcctta aaagatttaa cagaacaatt ggagacagta
    17221 gcccagtatg aaggccatct cttggatagt gcggataacc tactagaaat cactgaattt
    17281 tctcttgaaa tggaacgcca gatggagaag ctttacgttt atgctcatat gaagaatgac
    17341 caggatacac gtgaagctaa gtatcaagag tactatgcca aggccatgac actctacagc
    17401 cagttagacc aagccttttc attctatgat cctgaattta tggagattag cgaaaagcag
    17461 tatgctgact ttttagaagc tcaaccaaag ctgcaggttt atcaacacta ttttgacaag
    17521 ctcttgaaag gcaaggatca cgttctttca caacgtgaag aagaattatt ggctggagct
    17581 ggagaaatct ttggttcagc aagtgaaacc ttcgctatct tggacaatgc ggatattgtg
    17641 ttcccttatg tcctagacga tgatggtaaa gaagttcagc tatctcatgg gacttacaca
    17701 cgtttgatgg agtctaaaaa acgtgaggtt cgccgtggtg cctatcaagc tctttatgcg
    17761 acttacgaac aattccaaca cacctatgcc aaaaccttgc aaaccaatgt taaggtgcaa
    17821 aattaccgtg ctaaagttcg taactacaag agtgctcgtc atgcagccct cgcagcgaat
    17881 tttgttccag aaagtgttta tgacaatttg gtagcagcag ttcgcaagca tttgccactc
    17941 ttacatcgct atcttgagct tcgttcaaaa atcttgggga tttcagatct caagatgtac
    18001 gatgtctaca caccgctttc atctgttgaa tacagtttta cctaccaaga agccttgaaa
    18061 aaagcagaag atgctttggt agtcttgggt gaggattact tgagccgtgt taaacgtgcc
    18121 ttcagcgagc gttggattga tgtttacgaa aatcaaggca agcgttcagg tgcctactct
    18181 ggtggttctt acgataccaa tgcctttatg cttctcaact ggcaggacaa tctggacaat
    18241 ctctttactc ttgttcatga aacaggtcac agtatgcatt caagctatac tcgtgaaact
    18301 cagccttatg tttacggaga ttactctatc tttttggctg agattgcctc aactaccaat
    18361 gaaaatatct tgacggagaa attattggaa gaagtggaag acgacgcaac acgctttgct
    18421 attctcaata acttcctaga tggtttccgt ggaacagttt tccgccaaac tcaatttgct
    18481 gagtttgaac acgccattca ccaagcagat caaaatgggg aggtcttgac aagcgatttc
    18541 ctaaataaac tctacgcaga cttgaaccaa gagtattatg gtttgagtaa ggaagacaat
    18601 cctgaaatcc aatacgagtg ggctcgtatt ccacacttat actataacta ctatgtatat
    18661 caatattcaa ctggatttgc ggccgcctca gccttggctg aaaaaattgt ccatggtagt
    18721 caagaagacc gtgaccgcta tatcgactac ctcaaggcag gtaagtcgga ctatccactt
    18781 aatgtcatga gaaaagctgg tgttgatatg gagaaggaag actacctcaa cgatgccttt
    18841 gcagtctttg aacgccgttt aaatgagttt gaagcccttg ttgaaaaatt aggattggca
    18901 taaaatggtt gaatcgtata gtaagaatgc taaccataac atgcgtcgtc ctgtcgtcaa
    18961 agaagaaatt gtagacttga tgcgtcagcg tcaaaagcaa gtcacaggtt ccttgaaaga
    19021 attggaagac tttgcccgca aggaaaatat tcctattatt ccccatgaaa cggttgctta
    19081 tttccgtttt cttatggaaa ccatgcagcc taaaaatatt ctggaaattg ggacggctat
    19141 cggtttttca gctctcttga tggctgaaca tgcgccaaat gctaagatta caactattga
    19201 tcgtaatcca gaaatgattg gttttgccaa ggaaaatttt gcccagtttg acagtcgcaa
    19261 gcaaatcact ctcctagagg gagatgcggt ggatgtctta tctacactga cagagtctta
    19321 tgatttcgtc tttatggatt ctgccaagtc taaatacatc gtctttctgc cagaaatcct
    19381 caaacatttg gaagttggtg gtgtggttgt cttggatgat atttttcaag gtggtgatgt
    19441 tgccaaggat attatggaag tccgtcgtgg tcagcgaacc atttatcgag gccttcaaaa
    19501 attatttgat gcaaccttag acaatccaga actcaccgca acattagtgc ctttaggaga
    19561 tggtattctc atgcttcgta aaaatgtagc agatgttcaa ctgtctgaaa gcgaatgatt
    19621 ttcagaaaaa tttaagaaaa aatagtaaaa tagatagagt aacacttatc tcaaaggagt
    19681 agacatgaag aaaaaattat tggcaggtgc catcacacta ttatcagtag caactttagc
    19741 agcttgttcg aaagggtcag aaggagcaga ccttatcagc atgaaagggg atgtcatcac
    19801 agaacatcaa ttttatgagc aagtgaaaaa caacccttca gcccaacaag tcttgttaaa
    19861 tatgaccatc caaaaagttt ttgaaaaaca atatggctca gagcttgatg ataaagaggt
    19921 tgatgatact attgccgaag aaaaaaaaca atatggcgaa aactaccaac gtgtcttgtc
    19981 acaagcaggt atgactcttg aaacacgtaa agctcaaatt cgtacaagta aattagttga
    20041 gttggcagtt aagaaggtag cagaagctga attgacagat gaagcctata agaaagcctt
    20101 tgatgagtac actccagatg taacggctca aatcatccgt cttaataatg aagataaggc
    20161 caaagaagtt ctcgaaaaag ccaaggcaga aggtgctgat tttgctcaat tagccaaaga
    20221 taattcaact gatgaaaaaa caaaagaaaa tggtggagaa attacctttg attctgcttc
    20281 aacagaagta cctgagcaag tcaaaaaagc cgctttcgct ttagatgtgg atggtgtttc
    20341 tgatgtgatt acagcaactg gcacacaagc ctacagtagc caatattaca ttgtaaaact
    20401 cactaagaaa acagaaaaat catctaatat tgatgactac aaagaaaaat taaaaactgt
    20461 tatcttgact caaaaacaaa atgattcaac atttgttcaa agcattgtcg gaaaagaatt
    20521 gcaagcagcc aatatcaagg ttaaggacca agccttccaa aatatcttta cccaatatat
    20581 cggtggtgga gattcaagct caagcagtag tacatcaaac gaatagtcca aatcaatgag
    20641 tcagggaaaa aactcgactt caggaaaaaa tgaagcaaac attcccacaa taaaacgcat
    20701 agtacaaggt ttgtactgcc ccccccaaaa gttagacaat taatttatcc gaaggattta
    20761 gttctgtatt gcacagagct aagtcctttt agttttatct taattctctt gttgttgtaa
    20821 taatcaatat agtcta
//
LOCUS       DAVKQL010000039        20725 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVKQL010000039 DAVKQL010000000
VERSION     DAVKQL010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20725
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.039"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            254..574
                     /locus_tag="VW561_001375"
     CDS             254..574
                     /locus_tag="VW561_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9943626.1"
                     /translation="MQRLEVYKNYQHLYDLRMTILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKKEVEIYYQ
                     AKER"
     gene            complement(1077..1457)
                     /locus_tag="VW561_001376"
     CDS             complement(1077..1457)
                     /locus_tag="VW561_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0115 family bacteriocin-like peptide"
                     /protein_id="HEU9943627.1"
                     /translation="MELVLPNNYVALEQEEMMYLDGGGVGRNWWNSRGSFATVLDVGL
                     AIYSGGATIYSAYAIKKAISANRGAITRTLRSLIIKHVGSAAGHLVNTALNVALTVTG
                     FSLGGAIAYGADWADGSLDGYIFT"
     gene            2094..2957
                     /locus_tag="VW561_001377"
     CDS             2094..2957
                     /locus_tag="VW561_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9943628.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYLRQHGAGELSKHAEQVVKKLWKKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICTDNAKLIQKGFSLLELTEETSMLSHLKK
                     EVETHYQPKKL"
     gene            complement(3010..3597)
                     /locus_tag="VW561_001378"
     CDS             complement(3010..3597)
                     /locus_tag="VW561_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU9943629.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            3839..4510
                     /gene="sdaAB"
                     /locus_tag="VW561_001379"
     CDS             3839..4510
                     /gene="sdaAB"
                     /locus_tag="VW561_001379"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEU9943630.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            4519..5391
                     /gene="sdaAA"
                     /locus_tag="VW561_001380"
     CDS             4519..5391
                     /gene="sdaAA"
                     /locus_tag="VW561_001380"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEU9943631.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            5413..6045
                     /locus_tag="VW561_001381"
     CDS             5413..6045
                     /locus_tag="VW561_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9943632.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAMQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            6071..6169
                     /locus_tag="VW561_001382"
                     /pseudo
     CDS             6071..6169
                     /locus_tag="VW561_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(6392..8242)
                     /locus_tag="VW561_001383"
     CDS             complement(6392..8242)
                     /locus_tag="VW561_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU9943633.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVARAVDEAKVPKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(8281..9441)
                     /locus_tag="VW561_001384"
     CDS             complement(8281..9441)
                     /locus_tag="VW561_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9943634.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTESFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGFGTIVGLIRGFKWKKEYKGTIIYLDKISQINQNMLQ"
     gene            9499..10665
                     /locus_tag="VW561_001385"
     CDS             9499..10665
                     /locus_tag="VW561_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEU9943635.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTRLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPSVAALLLIDILMTVALFTVDRADKIL"
     gene            10804..11130
                     /locus_tag="VW561_001386"
     CDS             10804..11130
                     /locus_tag="VW561_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU9943636.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWALYTDTINGIIEGS
                     IRHDKN"
     gene            11117..11710
                     /locus_tag="VW561_001387"
     CDS             11117..11710
                     /locus_tag="VW561_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEU9943637.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLFNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTILAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            11703..12614
                     /locus_tag="VW561_001388"
     CDS             11703..12614
                     /locus_tag="VW561_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEU9943638.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHALTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILQLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            12677..13741
                     /locus_tag="VW561_001389"
     CDS             12677..13741
                     /locus_tag="VW561_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943639.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADLDTPIEK
                     NTQLEEEVSQAEVELESQQEEKIEAPEDSEARTEIEEKKASNSTEEESDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIVSILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPNQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(13813..14673)
                     /locus_tag="VW561_001390"
     CDS             complement(13813..14673)
                     /locus_tag="VW561_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEU9943640.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIYLVQITGDGSKPEDYESIARLDYAKFLEVLPPSFYHQLDANQIEIQPILG
                     QDFKTLAQEK"
     gene            complement(14795..15781)
                     /locus_tag="VW561_001391"
     CDS             complement(14795..15781)
                     /locus_tag="VW561_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEU9943641.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEDLWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(16053..17528)
                     /locus_tag="VW561_001392"
     CDS             complement(16053..17528)
                     /locus_tag="VW561_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEU9943642.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(17786..18709)
                     /locus_tag="VW561_001393"
     CDS             complement(17786..18709)
                     /locus_tag="VW561_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9943643.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(18723..19652)
                     /locus_tag="VW561_001394"
     CDS             complement(18723..19652)
                     /locus_tag="VW561_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9943644.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(19971..20192)
                     /locus_tag="VW561_001395"
     CDS             complement(19971..20192)
                     /locus_tag="VW561_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943645.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSQEELQKAVLF
                     LADEKGSEHTAAELIDNLKEVIAKLKANA"
     gene            complement(20146..20406)
                     /locus_tag="VW561_001396"
     CDS             complement(20146..20406)
                     /locus_tag="VW561_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943646.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKALTDDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
     gene            20486..20623
                     /locus_tag="VW561_001397"
                     /pseudo
     CDS             20486..20623
                     /locus_tag="VW561_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         6315 a         4399 c         3760 g         6251 t
ORIGIN      
        1 aaaattacgt taactttttt cacgaaaagg agaaaattat gcgttatgat tatggatagc
       61 gctttcaaat ttttaaactc ctatcccgtc cttttatcta tataataagc ataagcgaaa
      121 atataataac tgtcaagtat ctgaagtgaa ttttataaaa aaattacaag ccaaatttgt
      181 aaagtttaca ctaagccgct agggcaatcg tctatcagaa tgtccgttta tttgtcaata
      241 atccgagaaa atcttgcaac gcttagaagt ctataaaaac tatcaacatt tatatgactt
      301 gcgaatgaca attctattaa acctttccac actctatcta tacaatcaag ataaaaacat
      361 gtgtaagcaa atctgctaca ctttactaga ggacgccaag aataagaaaa gctacgatag
      421 gctggctatc tgctatgtcc gtattgggat ttgtacagac gattctaaac ttatccaaaa
      481 agggttctcc cttctggagc taaccgagga aacttctatg ctgtctcatc tcaaaaaaga
      541 agtagagatc tattaccaag cgaaggaaag ataaaaaagc cgagggattt cctcgactct
      601 ttacatatta tttgttcgtt agttcttaat accagccatt gttaagccag aagtttttag
      661 ctctgccaag ccacatagtg tgtttactac tatgtgtcca tgtcaccatg aatcgccgaa
      721 cgatttaacg agaaagtatg acttttacgt ttatcccaac tcaattatga catttttttc
      781 aaaagtcaat atatctcact ttttcaacga caagaaagag gctgataatc taccaacctc
      841 ttgtttttaa atacatttca agtccaaact attctttata gaaaaaagtc ctataaattc
      901 gagcaatagt taaaatacaa acaaatataa ttcccaaaat agataacact attttcacaa
      961 aattcatttc agaacctatt atatatacgt gaaaaaaagg ggctattcct acacatatat
     1021 acaataaata acttacaaag tttatttcac caattcctgc aaaaaaatgt ttcattttaa
     1081 gtaaaaatat aaccatctaa gctaccgtca gcccaatcag ccccatatgc gattgctcca
     1141 cctagtgaaa atccagtaac agttagtgca acgtttagtg cagtattgac taaatggcca
     1201 gctgcactac ctacatgttt aattattaaa ctacgtaatg ttctcgtaat agcccctcta
     1261 ttagctgaga tagctttttt tatcgcataa gcagaataaa ttgttgcacc accactatag
     1321 atggccaaac ctacatccag aactgttgca aaactacctc tactattcca ccagttacga
     1381 ccaacaccac ccccatcaag atacatcatc tcttcttgct caagagcaac ataattattt
     1441 ggtaatacta gttccattta ttttctcctt ttttattaag catgcttagg atggtttcct
     1501 aacaagtgat cgcaggcttt gtttagttac ctaaattaga cttccttcct ttatcttatt
     1561 ttaaatattt aaaaccagtt actgcttgaa gcttaacagt ttacttaatt caaataacag
     1621 ctaatcactg attccctgat tttatcaaat atactaataa caacaattgt gatacgatca
     1681 tcattataag aacaacattt aacgaatcaa agtttttccg agataataat aaagcaacag
     1741 caagcagtat tattactaaa gattgaataa ctactattac tcttaacatt ttatcctcta
     1801 ctaacaactc aagctccctt acattatatc gaccagcccc atggaaagat tatagccaga
     1861 ctaatcaata atataataca tactagaata caaaatattt ttttattcat actttttctc
     1921 ctatctttat tttctaccta catagcacct cttattaagc tcattttaaa attacgttaa
     1981 cttttttcac aaatttattt cgactttctt ttttactttt tacattgcta tgatacaata
     2041 tttttaacaa aattttattg tttgttaact tttttcacga aaaggagaaa atcatgcgtt
     2101 atgatttcgg aaaggtctat aaagaaatac gtgagtcaaa ggggctgacc caagaagagg
     2161 tctgtggggg tgttctctca agaaccagct tatcaaaaat cgaaagtggc aagacaaccc
     2221 caaaatatga aaatatggaa tttcttctcc gtcaaatcaa tatgagtttt gaagagtttg
     2281 aatatatctg tcaactttat caaccaagtc aacgcacaga gattatgcaa acctatctca
     2341 atatgcgttc cattatcggg accagcgatc ttgtcaattt atttcaaaaa tgccaagact
     2401 acctcaagac ccaccacgat ctccctatag aagaaatcag agatatgctt gaagttgtta
     2461 tttatctccg tcaacacggg gcaggggaac tatcaaaaca cgcagaacag gttgtcaaaa
     2521 aactttggaa aaaaattgaa aaacaagata catggtatga gagtgatcta aaaatcctca
     2581 ataccattct tttcagtttt cccattgaat acctccatct catcactgga aaaatcttgc
     2641 aacgcttaga agtctataaa aactatcaac atttatatga cttgcgaata gcaatcctgc
     2701 taaacctttc cacactctat ctatacaatc aagataaaaa catgtgtaag caaatctgct
     2761 acactttact ggaggacgcc aagaataaga aaagctacga taggcttgct atctgctatg
     2821 tccgtattgg gatttgtaca gataatgcta aacttatcca aaaaggtttc tcacttctgg
     2881 agctgaccga ggaaacttct atgctgtctc atctcaaaaa agaagtagag acccattatc
     2941 aaccaaagaa attataaaaa aagtcgaggg agctcctcga ccttttacat attatttgtt
     3001 cgttagttct taataccagc cattgttaag ccagaagttt ttagcagcag tccatgaacc
     3061 gtaacgtcct gcaacgtagg catctgctac acgttcttgg ttttcagctg agtagtcacc
     3121 gttcaggtat gaatctgtta attggtaacg tccgatataa cgtccatttg tagctgtata
     3181 gctaccacct gattcttttt gagcgatcca ttctttggct tctgcttcag atccgcttac
     3241 agttgaaaca actgtttcac ttactactgg agtttctgcc actggagctg aaacagtttc
     3301 atcttgagcg gctggtgccg cataagtagc tggcgctggt gttgcaacag gcgctacagg
     3361 gccatcgata accaactctt gatcaacata aatcaaatga atgttatcaa tgtggttgtt
     3421 ttctgccaat ttttcaactg ttgtgttgtg agtttcagcg atttctgaaa gtgtatcacc
     3481 ttctttaaca gtgtaagttg atgattcttg agcagataca aatgatggag caaatactgc
     3541 aaacaaggca gctactcctg caagagttgc tttaatcttt ttagttattg atttcatatt
     3601 cgaaaattct ccttctttct atagttctat catacccttt aaatattacc gtttcttgac
     3661 acttttatgt agaaatatta caaaattatt ttttatttcc ctaaataagg tgttttttgt
     3721 cataaaagat actattttta tgctatttga ggtattagaa aggttttcat ccacaattaa
     3781 agccaagccc ctcattcttt gatataatag agataagaat ttttataagg ggaaactgat
     3841 gaaatcactt cgttttcaat ctgtctttga tatcatcgga ccagttatga ttggcccatc
     3901 tagtagccat accgctggtg ctgttcgtat tgggaagatt gtctcttcca tttttgatga
     3961 tactccgaca gaagttgaat tccaactttt taactcattt gccaagacct atcgtggtca
     4021 cggaacagac ctagcccttg ttgcaggtat tttaggaatg gatacagatg atcctgaaat
     4081 tccaaatagt ctggaaattg cccacaagcg tggtatcaag attgtctgga ccattcagaa
     4141 agacagcaat gcccctcacc ctaacaccac taaaattact gttaaaaatg cccacaagac
     4201 catcagcgtg actggtattt ctatcggtgg aggaaatatt caggtaaccg aactcaatgg
     4261 ttttgccgtc tctctcaata tgaatacacc gactatcatc atcgttcatc aagatattcc
     4321 aggtatgatt gcccttgtta cagaggcgct ttcccgctat ggtatcaata tcgcccagat
     4381 gaatgtcact cgtgaaaaag ctggtgaaaa agccattatg attatcgaag ttgacagtcg
     4441 caactgtgat gaggctatcg aagaaattcg aaaaatccct catctccaca atgtcaattt
     4501 ctttaaatag gaggaagcat gttttattct atcaaagaat tggtcgagca agcagatcta
     4561 gactttcaag gaaatgtcgc agaactcatg attacaacag agtttgaatt gaccggtcgc
     4621 gaacgtgaag aagtcttcct tctcatggaa cgcaatctgg aagttatgaa agcctctgtc
     4681 caacttggcc tcaatgaaaa taaatctcgt agtggcctga caggtggaga tgctgccaaa
     4741 ttggatcact acatcgaaaa cggaaaaact ctgtcagatt atacgattct ctcggctgcc
     4801 cgaaatgcca tcgcagtcaa tgaacacaat gccaaaatgg gcttggtctg tgccactcca
     4861 accgctggaa gtgctggctg tctgccatcc gttctcactg ctgctattga aaaattagac
     4921 ctcagccacg agcaacaatt ggatttcctc tttgctgctg gtgcctttgg actagtcatc
     4981 gcaaacaatg cctccatctc aggtgctgag ggtgggtgtc aagctgaagt tggttcagcc
     5041 tctgctatga gtgctgccgc cttgactctg gctgcaggtg gaacacctta tcaggccagt
     5101 caagctattg cctttgtcat taaaaatatg ctaggcctca tctgtgaccc tgttgcaggt
     5161 ttggtcgaag ttccctgtgt caaacgtaat gccatgggag ctagctttgc tttcatcgca
     5221 gcagacatgg ccttggcagg tatcgaatct aaaatccctg tggatgaagt gatcgatgcc
     5281 atgtaccaag taggagcaag catgccaact gcctttcgtg aaacagctga aggtggactc
     5341 gctaccaccc ctactggtcg tcgcctccaa aaagagattt tcggagaata agcttatcaa
     5401 ataggagaaa ccatgacctc tatcacagcg atttttttcg atttggatgg aaccctcgtt
     5461 gatagttcta tcgggattca caatgccttt acctatacct ttaaagagct gggggtgcct
     5521 agccctgatg ccaaaactat tcgtggtttt atgggaccac ctctcgaaag tagttttgcg
     5581 acctgcctgt ccaaagacca aatttctgaa gccatgcaga tatatcgttc ttactataag
     5641 gcaaaaggca tctatgaagc tcaactcttt cctcagatta tagacttgct tgaggagtta
     5701 tcgagcagtt atccactcta catcaccacg acaaagaata catctactgc tcaagacatg
     5761 gccaaaaact tggaaatcca tcatttcttt gatggtattt atggttctag ccctgaagca
     5821 ccccataagg cagatgtcat tcaccaagcc ttacagacac atcaactagc accagaacaa
     5881 gccatcatca tcggagatac caagtttgat atgctgggag ctcgagaaac aggtattcag
     5941 aaattggccg tcacttgggg atttggagag caagcagatc tactaaacta tcaacctgat
     6001 tatatcgctc acaaaccctt agaagttttg gcgtattttc aatagcatag actgggcgac
     6061 aactctattt cagaagaaaa tgaagtaata ctcaatgaaa atcaaagagc aaactaggaa
     6121 actagctgcg ggctgctcaa aacactgttt taaggttgta gatggaactg acgaagtcag
     6181 ctcaaaacac tgttttgagg ttgcagatag aactgacgaa gtcagctcaa aacactgttt
     6241 tgaggttgtg gatagaactg acgaagtcag taaccatata cggcaaggcg acgttgacgt
     6301 ggtttgaaga gattttcgaa gagtataagt ctctacataa taaaacgcat attaccaagg
     6361 ttgttaactt gataatatgc gtttttcttt tttattcaat gtgggttgtt tgattagcaa
     6421 aggcgataat agcttgcttc aactcatctc cactgagagt gcggaactct ccaatctttt
     6481 gattgatgga ttctaaaggc atgacattaa ccttaccaac gaaaatctta tccataactt
     6541 gattgtcaac gagttcggtt gataccaaga gttcttcgta gagtttttca cctgggcgga
     6601 ttccaacttc aacgattgga atttcacttt cagtgtggcc acttagaagc accatcttct
     6661 tggccaagtc ataaatcttg actggtttgc ccatatcaag gataaagact tccccatctt
     6721 tggcataagc accagcatgg ataaccagac ggctagcttc tggaatggtc ataaagtaac
     6781 gggtcatgcg gaagtctgtc accgttacag gcccaccttc agcaatctga cgttcaaaga
     6841 ctggaatgac actaccacgg ctaccaagaa cattcccaaa acgaactgca cagtaggttg
     6901 attggctacg ttggttaaag ccagtgacaa tcaactccgc cacgcgcttg gttgctccca
     6961 taacatttgg tggattaact gccttatctg tcgaaatcat aaccatctta ggcaccttgg
     7021 cttcatcaac ggctctagca acattgtaag ttccacggat attgtttttg aaggcttctt
     7081 ttggattgcg ctccatcata ggaacatgct tgtgggctgc cgcatgataa acaatagcag
     7141 gtttgtactg ctcaaagact tgcaacaaac gatcatagtc ttgaatgtcc gcaatcacag
     7201 gtacataatc aatcccttgg aacttacgaa tcaattcatg ataaacaagg tagattgagt
     7261 tttccccatg accgagcaag acaatgcgtt caggattgaa gcgactaact tgacgacaga
     7321 tttcagaacc gattgaacct ccagctcctg tgactaagat ggtcttacct gtcagttctg
     7381 cacccagacg cgattcgtca agacggattt cctgacgacc caaaaggtcc gtaatatcaa
     7441 ttttttggaa gccagtacct gcttggtgaa ggccctgaac aacagtttca accttaggca
     7501 tcttgtaaca tttgacaccc agcttattac acatctgcaa gatacgctca tattctgacg
     7561 gatccagcga cggaatcgca acgatgacac gctcgatttg atggcgtttg gctaattcag
     7621 gcagattgtc ataagagccc aaaacaggaa taccaccaag tttttgaccc tttttcttag
     7681 aatccttatc caaaataccg accagttcta attcactggt tggatgttgg taactatcca
     7741 taaagagagc cccaccatca ccggcaccaa tcaagaaggt ccgacggtgt tctccatcac
     7801 cactaccttt tttgcgtctg gagtagatta actgccaagt aatccgtggc aataaaatca
     7861 agaaggtact caacaagata aagagaatga tgaaacggat ggagaagagt ggcaagaagg
     7921 cataacagat actatatgac aagacactgc tagcagtcac accaaaaaag attttcatga
     7981 aatccgtaat cttgctgtaa cgactaatgc tcgcgttcaa cccccaaaaa ccaatcatca
     8041 attgatagaa caagaaggcc aaactcgtat agataatgta gtcaacaggt gctggattaa
     8101 tcagcccata aaataaaata taagatacaa tgatggaaac caccatactg aaaatatcaa
     8161 atattcccca gaaaacctgc ttttgttgtt tatttaaaat ttccaccaga tcaatcacat
     8221 aatctgttag ttttttattc atagaaatct actcctcctt aaaagagttt tactatattg
     8281 ttattgtagc atattttgat ttatttggct tattttatcc aaataaatta ttgttccctt
     8341 gtactccttc ttccatttaa atcctctaat taaacctaca atcgtcccaa agccataagc
     8401 aaagtgaatg gaaaataaaa gaaagggcat cacaattaga aatccatttt tatgttttaa
     8461 taaagtcagc aaagtgagta gtgacaaaag tagaaaatag gcacctaata gtaaagttat
     8521 gaatacgaat gtgatcggta acaatgctag actaaacaca agactcaaaa caaataaaca
     8581 aggaacatag tgaaataatg ataaacactt aaactgaaca tgacttgtca agccaatcca
     8641 caaaccattt gaatactttt gatgcagcat tttcttgaat gttggtcgaa tatactgata
     8701 agatagaata cttgggctat agcggatttt ataaccatgt tctcgaattc tataatgaat
     8761 atcattatct tcagttcggc caagttgctc atttactaaa ccaaccttct ggaaaacctc
     8821 tcgtttatac atcccatgaa aaatagaaga aacatatcta tcctcggaac tatttcgata
     8881 attggcaatg ctactgccaa acatattttc ctcaacaaga tgcaaggtct ctgcccattt
     8941 tccttttcct tcgacaatcg tcggtctagg ccccccacag acaaattcac cttgttgaat
     9001 aatagccaca ttgttcatta caaaactctc agtaactttt gaatgagcat caatttttaa
     9061 aataaggtcc cctacagaat gtttaactcc caggttaaaa ccactagctt gatttttctt
     9121 aggattgtta tacaatctaa ttgagttaaa ctctgtatct tcctttataa attgctgaat
     9181 gatagctgtg gtcccatctg tggacatagc atttataaat agaatttcaa tattctcttt
     9241 aggataggtt tgatttttta agtcttcaat tagaccaggc agatattttt cttcattgta
     9301 tgcactgatt acaatggata cgagaagact ttcaacattt tgagtcacta ctactctcct
     9361 gaatttttcc ttccaccatt ctatcataat tttcaagact ttcccatttt tatcttgaaa
     9421 gccagaaacc ttacttgaca ttatagtgaa aaagccttaa aataagctgt tattaaaatc
     9481 agctagaaga ggtattatat gaaaaagcaa tcactctttt ttgttccagg tattatcctg
     9541 attggtgttt ccttgcgaac tccttttact gttttaccca ttattttggg aaatatttcg
     9601 caaggtctgg aggtagaagt tagttcgctt ggtgtcttga ccagcctgcc tctccttatg
     9661 tttaccctct tttcaccatt ttctacccga ctggctcaga aaatcggctt ggagcatctc
     9721 ttcacctaca gcctcttctt cttgaccatc ggctcactta ttcgactaat caatctgccc
     9781 ctgctctatc taggaacctt gatggttggg gcaagtgtcg cagtcatcaa tgtcctgctt
     9841 cctagtctta tccaagccaa tcaaccaaag aaaattggtt ttctgaccac cttatatgta
     9901 acgtctatgg ggattgcaac ggctctggct tcctatctag ctgtgcccat tacacaagcc
     9961 agttcttgga aaggacttat ccttctcctc acgttactct gtctagcaac ttttttggtc
    10021 tggctcccaa atcaccgcta taatcataga ctagctccac aaaccaaaca aaaaagtcaa
    10081 ataaaggtca tgcgtaataa acaggtttgg gcaattatta tcttttcagg ttttcaatcc
    10141 ttgatctttt acaccgtcat gacctggtta cctaccatgt ctatccatgc aggtctatcc
    10201 agtcacgaag ctggcttgct gacttctatc ttatctctga ttagcattcc tttttcaatg
    10261 accatcccaa gcctgacaac cagtttatct actcgcaacc gtcagctcat gctcactctg
    10321 gtttcactag ctggtgtggt cggcatttcc atgctctttt tcccaatcaa taatttcatt
    10381 tactggcttg ccatccatct cctcatcgga accgcaacca gcgccctctt cccttatctc
    10441 atggtcaact tttcactcaa gacaagcgcc cctgaaaaga cagcccaatt gtccggccta
    10501 tctcaaacag gaggctatat cctagcagcc tttgggccaa ccctctttgg ttacagtttt
    10561 gacctgttcc actcttgggt accatctgta gctgccctct tgctcatcga tatcctgatg
    10621 actgtggccc tctttacagt ggacagagcg gataagatcc tttaaacttc tagttttagc
    10681 tagaagtttt ttatatataa aaattttaca catttctctt gacagggctt tcttttagat
    10741 gtacaatgta tgtgtagaaa aattatatat aaaaatctta cacattagaa aaggaggttt
    10801 cccatgtact ttccaacatc ctctgccttg attgaatttc tcatcttggc cgtactggag
    10861 cagggtgatt cttatggtta tgagattagc caaaccatta agctaatcgc taatatcaaa
    10921 gaatccacac tctatcccat tctcaaaaaa ttggaaggca atagctttct gacaacctat
    10981 tctagagagt tccaaggtcg catgcgcaaa tactactcct tgacaaacgg tggtatagag
    11041 cagctcttga ccctaaaaga tgaatgggca ctctatacag acaccatcaa tggcatcata
    11101 gaagggagta tccgccatga caagaactga atacctgact cagctagaac tctatctcaa
    11161 gaaactacct gaagctgacc gtatcgaagc catggactat ttcagagagc tctttgacga
    11221 tgctggagtc gaaggagaag aagaactcat cgctagtttg ggaactccca aagaagcggc
    11281 ccacgaagtt ctattcaatc ttctcgataa aaaaatcaat gaagcacccg ctcaaaaaaa
    11341 taaccgacaa attttacata tcgccttgtt agccctcctt gcagcaccta tcggcattcc
    11401 tctgggaatc gccatcctcg tgaccctgtt cgcaatcctt gtagccgctt tgactgtcat
    11461 tctggctttc tttgcagttt ccatactggg tatcatcggc ggattcctat ttttagttga
    11521 aagtttcact atcctcgccc aagccaaatc agcctttatc ttgatttttg gttctggttt
    11581 actggctatc ggtgcttctt cgctagtttt acttggcatt tcctatgtag ctcgcttctt
    11641 cggtctactc attgttcgtc tggtacaatt tgttcttaaa aaaggaaaga gaggtaatca
    11701 gcatgcgtaa atggacaaaa ggatttctca tctttggtgt ggtgactacc gttatcggct
    11761 ttatcctgct ttttgtaggt atccaatctg acgggattaa gagcctactt tccatgtcca
    11821 aagaacctgt ctatgatagc cgtacggaaa agctaacctt tggcaaggaa gtcgaaaacc
    11881 tagaaattac tctccaccaa cacgcgctca ccatcacaga ctctttcgat gatcaaatcc
    11941 acatttctta ccatccatct ctttctgctc accatgatct tatcaccaat cagaacgata
    12001 gaactctgag tctcactgat aagaaactgt ctgaaactcc gtttctctct tctggaattg
    12061 gtgggattct tcatatcgca agtagctact ctagtcgttt tgaagaagtt attctccagc
    12121 taccaaaagg gagaactcta aaagggatca acatctcagc caatcgcgga caaaccacca
    12181 tcataaatgc tagccttgaa aatgcgaccc tcaatacaaa cagctatatc ctccgaattg
    12241 aaggaagtcg tatcaaaaac agtaaactca caacgcccaa tatcgttaat atctttgata
    12301 cagttcttac agatagtcag ctagagtcaa cagagaatca cttccacgct gaaaatatcc
    12361 aagtccatgg caaggttgaa ctgactgcca aagattatct cagaatcatc ctagaccaga
    12421 aagaaagcca acgaattaac tgggacatct caagcaacta tggttctatc ttccaattca
    12481 caagagaaaa gcctgaatca agaggtacgg aattaagcaa cccttacaaa actgaaaaaa
    12541 ccgatgtcaa ggatcaactc attgcgagat ctgatgataa tattgatcta atatccacac
    12601 caagcagacg ttgacaaaaa cgcttacatc tgctactatg gaagcatatt tatctaacta
    12661 aaaaggaggt tctaccatga aacaagaatg gtttgaaagt aatgattttg taaaaacaac
    12721 aagcaagaac aagcctgaag agcaagctca agaggttgca gacaaggctg aagaaacgat
    12781 agccgatctc gatacaccaa ttgaaaaaaa tactcagtta gaggaggaag tctctcaagc
    12841 tgaagtcgaa ttggaaagcc agcaagaaga gaaaattgaa gctcctgaag acagtgaagc
    12901 gagaacagaa atagaagaaa agaaggcatc taattctact gaagaagagt cagacctttc
    12961 taaagaaaca gaaaaagtca ctatagctga agagagccaa gaagctcttc ctcagcaaaa
    13021 agcaaccacg aaagagccac ttcttatcag taaatcttta gaaagtcctt atatccccga
    13081 ccaagctcca aaatctaggg ataaatggaa agagcaagtg cttgattttt ggtcttggct
    13141 agtggaagcg atcaaatctc ctacaagtaa gttggaaaca agtatcacac acagttacac
    13201 agcctttctc ttgctcattc tgttttctgc atcttccttt ttctttagta tctatcacat
    13261 caaacatgct tactatggac atatagcaag cattaacagt cgcttccctg agcagctagc
    13321 tcctttaact cttttttcta tcgtctctat cctagtagcg acaacactct tcttcttttc
    13381 attcctcttg ggtagtttcg ttgtgagacg atttatccac caggaaaagg actggacgct
    13441 agacaaggtt ctccaacaat atagtcaact cttggcaatt ccaatcttcc tcactgctat
    13501 tgctagtttc tttgctttct ttgatagcct acgatttaca gccctcttgt gtgtgattag
    13561 cattggaatc attctgcttg ccagtctcca tatcattaca agaccaaatc aagcaagtga
    13621 aaccgactcc ttctatcaat tattcttgtc tgtccttgtg aacggagtta ttatcctcct
    13681 cttctttgta gctgaagtgg cactgattgg agattatctt cgtatcttgg cctttcttta
    13741 aaattcaatc ctgtcatcct tggcaggatt ttttctatgt caaaaaagca agtcgattga
    13801 cctgcttctg ctttactttt cttgtgctaa tgttttaaaa tcttgtccta ggatgggttg
    13861 tatttctatt tgattggcgt ctagttggtg gtaaaaagat gggggtaata cctctaagaa
    13921 ttttgcatag tccaagcgag ctatggattc atagtcttct ggtttagagc cgtctcctgt
    13981 gatttgaacc aagtaaatct cccactggac ttccttatcc accaattgct caatataggc
    14041 tgcaggaaca aacggttctc tcggtaaaac tgtcttgact ccttgagcta aatggtaaac
    14101 gccatgcact tttccttctc catcctgata gaaggaaact cttgcaaagt aggcatctgt
    14161 ctcttgaacc gcacgcacac gcgcttcaaa ctgagccaag ccatctgcct ctaaaaagct
    14221 tttcaagttc tcacgactaa agaaaatagg attaaaaatt ccttggcaaa tcgtaaaacg
    14281 agctacaggg tctgctagat aggcttgaat acttgcttgg aaataagctt caaaatcaaa
    14341 accatctagc ccttcaatat cagtccctgt ataagcaagc aaagcatcta aaaatgactt
    14401 gataatctgc cagtctgtct ttgttagtag gtcaggaaga tcgacacggt aagccttttg
    14461 accatcagca taactgacct taaagagaaa ttgtgattgc cccactatcg cacactcaat
    14521 atactcgaga cgattaagag gctgacgaag atagactaca tcatagctat gtgactccaa
    14581 accgtctagc aaggccaaga tcgacttggc attcaaaatc tcttgttctc ctaaaatgct
    14641 ctgtttattt ggaataaaaa atgttttcgc cataactgga cccctttgaa gtcgtttaca
    14701 tacagtatac cttattttcc tgaaagatac aaaaacaaac tttagatttt tgagtacaaa
    14761 gcatagaaaa acagcccctg aggactgttt gatcttatac agtagctgcc tgatccaagc
    14821 tttcaccgat agcggctagg cgctcgatca cttcagcttg tgtcaattca ttttttgaaa
    14881 catagcggtt acgtgggtga acacggcact cgtgtgagca tccacgaagg tacttgtctt
    14941 cattttcttc tgatgtcaag atacgacggt tacagaaggg atttccacag ttgacataac
    15001 gttcacatgg cgttccatca aaccagtctt tccctacgat agttgggttg acatggttga
    15061 catcaacggc aatacgctcg tcaaagacgt acattttccc atcccaaagc tcaccttgaa
    15121 cttctgggtc tttaccgtaa gttgcgattc ctccgtgcaa ttggccaaca tctttgtaac
    15181 cttcacgaac catccagcct gagaatttct cacagcgaac accacctgta cagtaaacca
    15241 cgacacgctt gtccatgaat ttttctttgt tgtcacggac ccattgtggt aactcacgga
    15301 agttgcgaat atctgggcga atagccccac ggaaatgtcc taggtcgtac tcataatcgt
    15361 tacgtgtgtc aaggacaacg gtatctttat caagaagcgc ttctttgaac tcttttggag
    15421 acaagtaagc acctgttgtt tcaagtgggt tgatgtcatt gtcaaagtcg ttgtcttcca
    15481 aaccaaggtg gacaatttct ttcttgtagc gaacaaacat cttcttgaag gcttgttcat
    15541 tttcttcgtc aatcttgaac cagagatctt ccattcctgg aaggctgtga acgtagtcca
    15601 tgtatttttg agttgtttca tagtcacctg aaactgttcc gttaattccc tcgtcagcga
    15661 ctaggatacg gcctttaagg ccgattgatt tacagaaagc caagtggtct gcagcaaatt
    15721 gctctgcatt ttcaattgga gtataaaggt agtaaagtaa gacacgaata tcttttgcca
    15781 taagatttgt tctcttttct attcttaaat tttctaaatt ttttatttat ccatactagt
    15841 atacctgctt gagaaaacga atgcaattta tttgactgga aattgtagag ggttctcgaa
    15901 atttcctgaa tggttaaaat aaggacaaga gaaaacatgg atatctatat ccttgtgcca
    15961 aaaaaaccac tgccctcccc agaccaacct gaggaaagca gtgattctta ttttaggagt
    16021 taggaatgaa tacacgaaat caatttagct gattattttt tgtttttcaa gaattcatcg
    16081 tattgttttt gcatttcgtt caatactttt tcgtaggcac cttcagattt caatttttcc
    16141 atcaattctg gaatcgcttt atctgggtct acagtaccag tgttgatagc tgtatcaaat
    16201 tgttgcattg tgttagcaat agctgagatt tcagatttca cattgtcagt attgaagata
    16261 aatccaagcg ctggagattc tttagcttct gccaattctt tcttagaatt ttcgatttgt
    16321 tggtctgtaa cgttttcgtt gatgtaaagg atccagttgt taccagtgtt ccatccaccc
    16381 atgtgagtgt ttcctttgta gccatcaaga acgcgaacac ggttttcttt accttcaatt
    16441 ttttcccagt tcttgccttc tggaccgtaa acaagaccgt tcaagagttc tggattcgta
    16501 ttcaagaggt tcaagatttc cattgatttt tctttgttct tagagttgtt tgagatgaca
    16561 aagttagcaa cttgtgttgt ttggtttttc ttgatgaagt tagtaattgg tttgatttgg
    16621 atatctttgt tggcaacacg tgaaagcaag ctgttaccgt agtcagctgg tcctactgtt
    16681 tcttcacgaa cgaaccaagt atcttgttga aggtcaaagg aagtatcgct tgttgcgacg
    16741 tcttttggaa tgtagccagc ttcatagaat ttgtgaagag tcttcaagtg ttctttgaaa
    16801 cgaggcactt cgtaacggtt tacaacttta gtagtatcgc cttcaaggtc gataacgaat
    16861 ggaagaccgt ttgctactgg gtagtcaaag ttatcagatg ggatgaaaac tttaccaata
    16921 gcaaatggta ctacgtctgg agctttttct ttaatttgtt tcaagactgg ctcaagagtt
    16981 tcgtaagaag taacacctga aatatcgata ccatatttag caaggagagt tccgttgaag
    17041 gcaaagtttt gagatgatgc aacgttggct gcaactggaa cagcgtaaat cttaccattt
    17101 acagtattac ccttgatgta agctgggtca agtgctttgt aaaggtcttt accttctttt
    17161 ttgtacaatt ctgtcaagtc agcgtaagca cctttttgag catttacaat atagttatct
    17221 gcaaaggcaa tatcatagtt ttcaccagat gatgtgataa ctgacatttt cttaccatag
    17281 tcaccccagc caaggtattg gatatccaat ttggcaccaa ctttttcttc aatgattttg
    17341 ttggcatttg ctaacaattc atccaagttg tctggtttgt caccgatttg gtacattttg
    17401 ataacaggtt tgtcacctga atcagcagct tttttgctgt tacctgtcaa atttccacaa
    17461 gcagcaagac ctgcagccaa agcgactaca ctagcagatg caaaagcata ttttttccag
    17521 tttttcatga taaaaactcc tttttttatt tttaaactta taaacaatgt aatgatctta
    17581 tactcaatga aaatcaaaga gcaaactaga aaactagccg caggctgctc aaagcactgc
    17641 tttgaggttg tagataagac tgacgaagtc agttacatat atctacggca aggcgacgtt
    17701 gacgcggttt gaatttgatt ttcgaagagt attaacttca cacaagggaa gttgggaact
    17761 gagaaatgtt ctttttcaat aagcactatt ctttcacacc accgatagtc aaacctttta
    17821 caaagtagcg ttggaagaat ggatacaaaa tcgcgattgg aagggttgct accacaacca
    17881 tggccatacg acctgtttct ttcggtagag caactcccag ttgaccagtc aagccgaccg
    17941 ctttggcaat gtagtccata ttttgttgga tttgcatgag taaatattgc aatggataca
    18001 agttgtcact cttgatgtaa agaagggcgt tgaaccagtc attccagaaa ccaagagctg
    18061 tcaaaagcgt gatggttgcg atacctggta gtgacaatgg caaacagatt tggaagaaaa
    18121 tccgggcctc actggcacca tcgatacgag ccgattcgag aatggcttct ggaatggtct
    18181 tcttgaaaaa agaacgcatc aagatgatat tgaatggtga gagaagcatt ggaacaatca
    18241 aggcccaaac agtgtcacca agctgaagta cacgggtcac catgatataa cctggtacca
    18301 aaccagcgtt gaacaacata ctgagaagga cgaagatggt aaaaaatctg cgatacttaa
    18361 aggttgtccg tgaaatagcg taggcatagg ttgttgtgat aaagacattt gtcaatgtcc
    18421 caactacggt tacaaagaca gagatgaaga gggcttgtag gattttatcc ttaaactgtg
    18481 ccaaaaactc aaaaccgtct aagccaaatt gggatgggaa gaagctatag ccgtattgga
    18541 ggaggctttt ctcgtctgtc actgaaataa tgataacgaa tacaaatggt aggatacaag
    18601 agagggcaat caaacccgaa atgatactga agaagatatc tgctttctta ctgaaggagt
    18661 gaatgccgac attatcaatt ttttcttttt taattttctt ttttgccata ttctcctcct
    18721 ttctagaaca aagctgagtt tggatcgact cgtcttgcaa gcaagtttga taggataacc
    18781 agaatcaaac caacaacgga ttggtaaaga ccggctgctg cagccatacc gatatctgct
    18841 gtctgagtca aaccattaaa gacatatacg tccaaaacgt tggttacatt gtaaagctga
    18901 ccagcattgt gtgggatttg atagaagaga ccaaagtctg cgcggaagat atttccgact
    18961 gcaaggatgg tcaatacagt tacaagcgga gtcaactgag gaatggttac gttgcgaata
    19021 cgttgccact tgctagctcc gtccactgtc gctgcttcgt agtaggttgg atcaattccc
    19081 atgatcgtcg catagtacat gacactgcta tatccaaagc ctttccaaat acctaggaaa
    19141 agtaggagat agggccagat gcccaggtca gcgtaaaaat tgacttcttt gagaccaaga
    19201 ctttccaata gatgattgaa caccccttta tcaatattta ggaaggcatc tgtaaagaaa
    19261 ctgatgataa cccaagacaa gaagtaaggg aacaacatag aagtttggaa aatcttcacc
    19321 attctcttag aacggagctc gctgaggata atggcaatcc ctacagatac aactaaacct
    19381 agaaagataa agccaagatt gtagaggaca gtatttcgtg tgataataaa ggcgtctctt
    19441 gaactaaata agaatctgaa attatcgagt ccgacccatt tactatttat gatactatct
    19501 atgaaaccat tactggtcat gtggtagtct ttgaaggcaa ccacgttccc aaatactgga
    19561 atgtaaaaga atagaatcaa ccagagtgcc cctggcaaaa ccatcaagag aaagatccag
    19621 ttgtctctca atgtttttga aaactttttc ataatttcct ccctttttat tttgatatcc
    19681 atctaaaaat tcttttttag acttttgata acgattacat tattagtata ctcctatttg
    19741 caggttaggt taaactccta attatagaaa aaactccaca aattatgtag cagatttaaa
    19801 actttatcac cactatcaaa caaatgtcct aaatcaattg tttattttat ctctattagc
    19861 ccagtgatgg cgtcactctg ttataagcat ccaacaacgg ggtatactga aaaatctcca
    19921 gactagggaa ctcagcgata gttcctaatc tggagatttt taatatgtta ttaggcgttt
    19981 gctttcaact tagcaataac ctctttaaga ttatcaatca actctgctgc agtatgctca
    20041 gagccttttt catctgccaa gaacaaaact gctttttgaa gttcttcttg agagttttca
    20101 aggacatcct tatctactgt ttcaaggttt gagtctttaa gaagtttact taattccttg
    20161 gctaatttct tgagtttgat ttgcagactc atcttctcct gctgtttctt tgcccgctgt
    20221 ttgtcctcca tccttagttg ctgactggct ttccttaatg gactctaggg aagcaatggc
    20281 atctttgact gtttgcaaga tatcacgtaa accttgctct gtcaaactat catctgtaag
    20341 agctttctta gcctctgcca aaaccagacg tgctgaatct gtggtaggat tcgatacacc
    20401 tgtcaatgat ctcaaaagat tttctaaagt ttgagtctgc ttactaatac tagactaaaa
    20461 tcaaaaagca ttatataaca gtgatatgaa atcaactaaa gaagaaatcc aaactatcaa
    20521 aacactttta aaagactctc gtacagctaa atatcataaa cgccttcaaa tcgttctatt
    20581 tcgtctgatg ggcaaatctt ataaagagat tatagaactt ttatagtagt ttgaaataag
    20641 atgtgaacaa ctctatcagg aaagtcaaat taatttatag aaatatttta gcagctaagg
    20701 tgtactgtta tagattcaat acact
//
LOCUS       DAVKQL010000040        20121 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVKQL010000040 DAVKQL010000000
VERSION     DAVKQL010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20121
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.040"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(<1..619)
                     /locus_tag="VW561_001398"
     CDS             complement(<1..619)
                     /locus_tag="VW561_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEU9943647.1"
                     /translation="MDFEYFYNREAERFNFLKVPEILVDREEFRGLSAEAIILYSILL
                     KQTGMSFKNNWIDKEGRVFIYFTVEEIMKRRNISKPTAIKTLDELDVKKGIGLIERVR
                     LGLGKPNIIYVKDFMSIFQVKENDLQKSKNLTSEVKDFNLRSKENELQEVKNLDSNYI
                     ENNKSKYSKREYSFGENGLGTFQNVFLAAEDISDLQIIMNSQLENY"
     gene            complement(728..1246)
                     /locus_tag="VW561_001399"
     CDS             complement(728..1246)
                     /locus_tag="VW561_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEU9943648.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            complement(1467..2336)
                     /gene="rsmI"
                     /locus_tag="VW561_001400"
     CDS             complement(1467..2336)
                     /gene="rsmI"
                     /locus_tag="VW561_001400"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEU9943649.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFDS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQGVEEKDEEDLFVEIQTRIQQGVKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            complement(2339..2656)
                     /gene="yabA"
                     /locus_tag="VW561_001401"
     CDS             complement(2339..2656)
                     /gene="yabA"
                     /locus_tag="VW561_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEU9943650.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            complement(2696..3586)
                     /locus_tag="VW561_001402"
     CDS             complement(2696..3586)
                     /locus_tag="VW561_001402"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEU9943651.1"
                     /translation="MKQDQLKAWQPDQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            complement(3583..4221)
                     /locus_tag="VW561_001403"
     CDS             complement(3583..4221)
                     /locus_tag="VW561_001403"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEU9943652.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            complement(4337..5134)
                     /gene="proC"
                     /locus_tag="VW561_001404"
     CDS             complement(4337..5134)
                     /gene="proC"
                     /locus_tag="VW561_001404"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEU9943653.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSELFCQLLSKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLSRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            complement(5138..6400)
                     /locus_tag="VW561_001405"
     CDS             complement(5138..6400)
                     /locus_tag="VW561_001405"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEU9943654.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASILPRLDQ
                     MLVAERKEAGLEPIQFRLDSKASQFVSGQAAETQDFDTEFLDYVLAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEVAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            complement(6410..7540)
                     /locus_tag="VW561_001406"
     CDS             complement(6410..7540)
                     /locus_tag="VW561_001406"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEU9943655.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGAFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            complement(7620..9503)
                     /gene="cbpE"
                     /locus_tag="VW561_001407"
                     /gene_synonym="pce"
     CDS             complement(7620..9503)
                     /gene="cbpE"
                     /locus_tag="VW561_001407"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEU9943656.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDNRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRQDGLVNISTSYKPIPS
                     FQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNNRWFY
                     LTDSGVAAKNWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTDGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            complement(9506..10393)
                     /locus_tag="VW561_001408"
     CDS             complement(9506..10393)
                     /locus_tag="VW561_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9943657.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLALAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            complement(10383..10844)
                     /locus_tag="VW561_001409"
     CDS             complement(10383..10844)
                     /locus_tag="VW561_001409"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEU9943658.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRASQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            complement(10841..11749)
                     /locus_tag="VW561_001410"
     CDS             complement(10841..11749)
                     /locus_tag="VW561_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU9943659.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKSQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLREGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            11742..11930
                     /locus_tag="VW561_001411"
                     /pseudo
     CDS             11742..11930
                     /locus_tag="VW561_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(11982..12653)
                     /locus_tag="VW561_001412"
     CDS             complement(11982..12653)
                     /locus_tag="VW561_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9943660.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETVEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(12707..12928)
                     /locus_tag="VW561_001413"
     CDS             complement(12707..12928)
                     /locus_tag="VW561_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943661.1"
                     /translation="MILMTKNINLTNEELELIQGGADPYGKEPNGYYPWQMEPVLTMP
                     VHGFCPRGTDDLGYIGGGNHLCKGSAARF"
     gene            13201..14010
                     /locus_tag="VW561_001414"
     CDS             13201..14010
                     /locus_tag="VW561_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9943662.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLELDLQNEYIIVNNGCSTHQTSDWSLVDWKELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(14191..15066)
                     /gene="aguB"
                     /locus_tag="VW561_001415"
     CDS             complement(14191..15066)
                     /gene="aguB"
                     /locus_tag="VW561_001415"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEU9943663.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGGVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(15076..16161)
                     /gene="aguA"
                     /locus_tag="VW561_001416"
     CDS             complement(15076..16161)
                     /gene="aguA"
                     /locus_tag="VW561_001416"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEU9943664.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRSF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAELVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEKRYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(16158..17285)
                     /gene="nspC"
                     /locus_tag="VW561_001417"
     CDS             complement(16158..17285)
                     /gene="nspC"
                     /locus_tag="VW561_001417"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEU9943665.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCHILQYVQEEVGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDADLEELLGITDHIVF
                     NSERQLRKHGSRCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFEAQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(17285..18544)
                     /locus_tag="VW561_001418"
     CDS             complement(17285..18544)
                     /locus_tag="VW561_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEU9943666.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            complement(18541..19401)
                     /gene="speE"
                     /locus_tag="VW561_001419"
     CDS             complement(18541..19401)
                     /gene="speE"
                     /locus_tag="VW561_001419"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEU9943667.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQSFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGSFMLPKYVE
                     DILEEEEGKK"
     gene            19657..>20121
                     /locus_tag="VW561_001420"
     CDS             19657..>20121
                     /locus_tag="VW561_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEU9943668.1"
                     /translation="MLYVGIDVAKNKHDVTALNVQGKTVLKPLTFSNNKAGFELLDLS
                     LRQLNQDCLIALEDTGHYAFNLLNFLHEQGYKVYTYNPLLIKKFAKSLSLRKTKTDKK
                     DAHGIALKLLSDPNREQFQHNNRQVELKILTRHIHRLKKKQSDWKVQYTRCLD"
BASE COUNT         5828 a         4637 c         3583 g         6073 t
ORIGIN      
        1 tgtaattctc aagctgtgag ttcattatga tttgtaaatc cgatatatct tcagcagcta
       61 aaaacacatt ttgaaatgtt ccaagtccgt tttcaccaaa actatattct ctcttactat
      121 acttactctt attattctct atatagttag agtcaaggtt cttaacctct tgaagttcat
      181 tttctttact tctgaggtta aaatctttta cttctgaagt taagtttttt gacttctgta
      241 agtcattttc ttttacctga aatatactca taaagtcttt aacataaatg atgttcggct
      301 taccaagtcc aagccttact ctttcgatca gtcctattcc tttttttaca tcaagctcat
      361 ctaatgtttt tatggcagtt ggctttgaga tatttcttct tttcataatt tcttcgacag
      421 taaaatagat aaatactctg ccttccttgt ctatccagtt attcttaaat gacattcctg
      481 tctgtttaag aagtatggaa taaaggataa ttgcttctgc tgataagccc cgaaattctt
      541 ctctatcaac taatatctcc ggtactttta agaagttaaa tctttccgct tctctgttat
      601 aaaaatattc aaagtccatg caagatcacc tcccttctta aaaatttgca aagaaaaaga
      661 cgatagtttt tcttctatcg cctttggtaa ccatttttat gaaatttttt aaagttaaaa
      721 atataattta taatactatc ttcttccatt ttctgatttt agttctgaaa gttccaaaag
      781 gtgcaaccgt atttacatga ataaacttat acacttccca agtcgctgtt ttagtcgctt
      841 catcagccca ctttctcata tgcggttcaa ataattcttc ctcactcaac gaatcaatca
      901 ttgcagagat agaattaata ttttcattta attttgcttt caactcttgc agagataaat
      961 gagcgtaggt atctgtgaac cactgatata attcaccaag ttgattccat ttaaatttat
     1021 ccgatggtgt ttttacttga agcccctttc tttcatcttc ttcccattta agaaccaagt
     1081 tggtccaacc aacctgataa gcaaggtttt ctgctggagt tctgtcaact tcatcagctc
     1141 tcttatcttt taaattttct ggaatattat caaattctaa aatatatttc tcaaatgttt
     1201 tctctatctc agcttttagt tcttctttat tttcgtatgt tcgcaaaaaa tcaccttctt
     1261 tctgatttat caatcctttg atttaaaatg accttatttt tctatttata ccttctagga
     1321 acgctataac tgctgctcca accatcggta ttccgtatgg gcttaataaa aaacttaata
     1381 ccaaagcttc tataccaagt gatacatctt tatattatca tcactaatta agttaaacag
     1441 acagaattat tacatcctgt ctccctttat tgtttttctt cccagtcgtg gtaggcagcg
     1501 tagagctgac ttttattcca ctggtaaatc ttagcgactt ccttgatagc ttggtttttc
     1561 ttcacacctt gctggatgcg ggtttgaatt tctacgaaca agtcttcctc gtccttttcc
     1621 tccacaccct gactggcacc ctcaacaatg agaagacatt cgcccttgag tggcgtttca
     1681 gcaatgcttt ctaataactc agagatagta cctcgttggt attcttcata gattttggtc
     1741 aattccctga ccaagacaac ggagcggtca ccgtagactt ctagcatatt ttccaatgta
     1801 tctgccacac gatggggcga ttcataaaaa atctgagttt caggatagtc ttttttcgag
     1861 tcgaaaaatt gcttttgctg gcctgatttt ctcggtaaaa agccgtaaaa gatatgtggc
     1921 tgcggcgcta aaccactggc aatcaaggcg gaaattcctg cagaggcacc tggaactgta
     1981 acaactgcaa tttcttcctc aatagctgcc ttaaccaaat catgaccagg gtctgaaatg
     2041 ctaggcaaac cggcatcaga gacctgagca atactttgcc ctgctttcaa gaaaccaatc
     2101 aaatcaggaa ttttttcctt ggcattgtgc tcatgaaaac tgatctgctt ggtggaaatg
     2161 tcaaaatgct tgagcaaaag acctgtattg cgcgtatcct cagcagcaat ccaatctact
     2221 tctttcaagg tctggatagc tcgaaaagtc atatcatcta gattgccaat cggcgttgcc
     2281 actagataca gcttgccata gggagactgc cccttaaaac ttttttgaat ctgcatgcct
     2341 actccctgta tagcaactcg tcacaaaaca tacattcctc gtcctgctct cgacgttgtc
     2401 cataaaaatc attacatacg tgaaatccat cacggtaaat acgacggaca ctttcgcgaa
     2461 catgcttggc cttgacggga gcgtctgctt ccacctcacc caagcgttct cgcaacttac
     2521 tattttccaa gcgaagagct gtattttctt ctaccaagct cttgagattt ttcttgatgg
     2581 cttccacatc ggctaaggtt accaataatt gttgggaaaa atcatccagc gcgtcaaata
     2641 attctttttt gtccataaaa cagccctttc ctttctttat cattcatttt tgagtttata
     2701 tttctttcaa gaccagatat tccatggcat tttgaaagct gacattagct tgccacattt
     2761 ttctagcttc tagtaaatct tgtagaatca ctcttactct tacctgcaag aggtcctgcc
     2821 cacagagaac ttcaagaatc cgtaaaacct gatcctgttt ttccttatca tctgccaagt
     2881 tggctaattt ggcaacctgt agataacttt cttttttctt agctactaac caagtcagca
     2941 ggcgttcact ttcatcgacc aaggtccaaa aacttgcctg attagccaac ttttctgctt
     3001 cagctcgcga ttgactaaac ttagctaaaa gagtcgcttt tttcttaaca agtcccattt
     3061 gttctaagag taagataagt ttttcttctt gctttttaaa gtggaagatc tgagtccgac
     3121 ttcggattgt cggtaacatc ttttcctcat cgctagtcaa gaagaaaata taaacttcac
     3181 tctggggttc ttcgatgacc ttgagcagag aattggctgc gttgggatgc attttatccg
     3241 cttgctcgat gataaagacc tgttgctggc tttcaatccc tgcttgagaa aactgtccca
     3301 ccaattcccg aatgcgttct gtcttgatga cctgattgac tggcttaatc aaggtgacat
     3361 ctggaaactc ttcctgttca atcagcttgc aacttcggca tttctcacat ggtaagacgc
     3421 caactttatc cgtacaaaag aggctcttag ctaaaaattg cgccatttcc aagcttccaa
     3481 agaaacctga aaagagatag gcgtgattga gctggtcttg ttctaagata cggacaaaac
     3541 ggtcaaactg atctggttgc caagccttta gttgatcttg tttcatttgg ccaagcccat
     3601 tccgtcaaac aagacagcct tggtagtttc cacaacttgc tccaaaggga gactagcatc
     3661 aatcttgaca atgcgatttc cctctttatc cagaagagaa aggtagcctt gacgaacttt
     3721 tttatgcaag tccaaccctt ccaaatccaa acgattaacc tcgcggtcac tattagcagc
     3781 aatacgagcc agcccttctt ccacctcgat gtcaaaatag agtgtcaaat cgggtttgag
     3841 accatctgtc gcaaactgat tgagccagtc aatggcttca atatctaagc cacgaccaaa
     3901 tccctgatag gcaacagaac tatcgataaa acgatccatg atgaccaact tgccagcttc
     3961 aagggctgga agaacttttt ccaccaaatg ctgtctgcga ctggcaatat agagaagtag
     4021 ctctgtttta gcatccatct gagtatgact tggatccaaa atcacttccc gaatcttctc
     4081 cccaatcaag actccgccag gttcacgggt cgtcaacacc tctactcctt tttcctctaa
     4141 aattggtagc agagcctcta aaacactggt cttgcctgct ccctctggtc cctcaagaga
     4201 gactaaaaat ccttttgaca tgtctaactc atttcttttt tactacttct attctatcaa
     4261 aaaaataggg gtttgtgaca atctttttgt gtcttctcat ttcagccacc ataaaagagg
     4321 cagtcaaagc tacctcttat ttacctagtt cttgtgttcg tttataggct tgatgaactg
     4381 cctccatgac tgtgcctcga aaagcatgcg cttctaggct tgctacgcca gcaatagtcg
     4441 aaccgcctgg actacaaact tgatctttca aaactccagg atgttgctgg ctttctagga
     4501 ccaattgccc agctcctacc acagtttggg ctgccatttt caaggctgtt tctcgtgata
     4561 atcccgtctg aacacctgca tctgccaagg cttcaataaa gagatagaca aaggctggtc
     4621 cacagcctgc aagacctgtc gctgcattga ttaagctttc tcctaattca accaagagac
     4681 cagccttgga taaaagctga caaaagagct cactgtcctc agccctgcaa ttaggagaca
     4741 aggcataact aatcactcct tgcccgatag aagcaggggt attaggcatc atacgaataa
     4801 ttcggtgttg acttgggata agacttgcta gtttttctaa ggtcaatcca gctgccatcg
     4861 aaatcaaaag aagactttct cttttttcaa ggatggtctg gtattgagaa agcagttcag
     4921 aaaactgagc aggcttaact cctagaaaaa tcacatctgc ttctgcaaac atttcttcat
     4981 tgctggaagc ctgaccacca aagtctgcaa tgaaagcatc taccttagct tgactacgat
     5041 tggcaaggag aatctcatct gacgtcctag tctgcaagac agattttgcc aagctagcac
     5101 ccatattccc caaaccgata aatccaatct tcatctctta ctcccttatc tgcccatcac
     5161 cagcaaccac atacttgtag ctggtcaact ctttcaagcc cattggacca cgcgcgtgca
     5221 atttctgagt agaaatcccc atttcacaac caagaccaaa ttgtcctcca tctgtgaaac
     5281 gagttgaggc attaacatac accgctgcag agtccacttg atctgtaaag tatgctgcaa
     5341 cttcagcatt ttccgtcaca atagcatccg aatgatgggt gctgtgggat tcaatgtgcg
     5401 caaccgcttc ttctaaactg ctcacaacct taacagcaag gacatagtct aaaaactcgg
     5461 tgtcaaagtc ttgggtctca gctgcttgac ctgaaacaaa ctggcttgct ttgctatcca
     5521 agcggaattg aattggttcc agtccagctt ccttacgctc tgcaaccagc atttgatcca
     5581 agcgaggaag gatgcttgct gccttgtttt catgaaccag cagaacctcc atggcattac
     5641 aaacagaagg acgactggtt ttagcattgt tgatgataga cagcgccttg tcttcgtctg
     5701 catccttatc cacatagaca tggacaatcc cagtccctgt ctcgataaca ggtacaatcg
     5761 cattctcaac cactgcattg atcaagccag ctcctccacg aggaatgaga aggtctagat
     5821 agcccttggc cttcatcata gcataactac tttcacggct agtatcctcc accagttgaa
     5881 tcacatttgg atgaatagta gtcgtctcca agcccttctt caaggctgtg acaatggcat
     5941 gggttgtttg ataggcatcc ttaccactac gaagaacaac cgcatttcca ctcttaagag
     6001 tcaaagcagc cgcatcagac gtcacatttg gacggctttc atagataata ccgatgacac
     6061 ccatagctac acgttttttt gtgataacca aaccattttc aagctgactt gtttctaaaa
     6121 cttcaccgat tggatctggt aaggcaacca cttcacgaat tcctcttgcc atcgcttcta
     6181 tacgatctgc atccaaataa agacgatcca acatcacatc tgagattttc cccttagccg
     6241 ctgccatatc gagggcatta gccgctaaaa tttcctcagt agcagccact aagtgatcag
     6301 ccatggctag caaggcttgg tttttcactt cttcactagc tgtgttaatc gattttttaa
     6361 cagcctgtac ctgttcaaat tgttcttgtc tactcaccat agtttacctc taaaattctg
     6421 taaaaagtag ttggatttca ggagtaatgg aaatccagtc gtcacggtaa atcaagacac
     6481 ccttggcttt ttgagaacgc aacatatcct ccaaagcaga tgctccaaat tgcacgcgtc
     6541 cttttccaag tgattttcca ctttccttgt caaatactgt cacgatatca ccgtaagaaa
     6601 aggctccttc tgcttcaacg ataccagata agagaagact ctttccatgt tgagagagag
     6661 cttccgcagc ccctttatca acccaaatag aaccttgact ctgagcatag aaggcaagcc
     6721 attgtttctg ggtacgaagc cccttctctt gagcaacaaa gtaagaacca tcctcggtct
     6781 cctctgccgc ctcaatcatg gaatctgatt tcaaggatga gcagatataa acaggaactc
     6841 ctgattccgt cgcgatagtt gcagccttga ttttggttaa cataccccca gttccgtttg
     6901 acgaacctgc tccaccagcc atatcaataa tctcacgatt gatggtctcg attctctcca
     6961 agcgtttggc tcttggatct gaattaggat ttccagtata gagaccgtcc acatctgtca
     7021 agaaaactaa aaggtctgct tggaccatcg ccgctacttg agcacttaga gtgtcattgt
     7081 ccccaacctt gagctcatca ataacgacac tatcattctc attgatgata ggaattgccc
     7141 cacggttgag caaaaccgac aaagcctgat gggcattttt ataacgacgc ttatccacaa
     7201 agtcatcttg ggtcagcaag atttgtgcag aaacgatttg acgcaagaga agattggtcg
     7261 tatattcttc caacaaaagt ccctgcccta ccgctgctga agcctgttta tcagcaattt
     7321 tagtcggacg ctttttaaat cctaaggctc caaaaccagc cgcaatggca cctgaagaca
     7381 ccaaaatcaa ctcatgacca gcctcgtgca gcatagccaa ctgctgggta atatccttta
     7441 ccttactacg tgataaactt ccatcctcat ttgtcagaga agaagtaccc accttaaaga
     7501 caatccgttt gtatttcata gtctcactcc gattattcat atttatccat tataacatga
     7561 ataacaggaa atagaaaaga gttgatagga aaaagaaggc cctaaaacct tcttttctac
     7621 tactgttctg attccgattt gtttacactc gtttcagagc tttgtgaagt tgaggatgtc
     7681 aaatcttgac tagttgaaga atttgtaaca ctttcatgtt gactcgtttc tttattttca
     7741 agatcctttt tcactacttc tttggattct tttatttctg aatgacttgt agttttaatt
     7801 gtagcagatt tctcgatcgg aacatccact aaccaggcac cacttgaatc cacagtatag
     7861 ccttctggtg tcttaccatt cacaagtaat tgaccatttt ctttcaagaa gtaccattta
     7921 tccttgtctt taatccaacc tatagctcgt gaaccatctg tcttgtagaa ataccaatca
     7981 tttgcctttt taagccagcc ttgcttcata gctcctgatt cttctagata gtaatgatgt
     8041 ccagcaactt ctatctcacc tgtcttcatg ataccagtag aatccatata gtaccaggct
     8101 tctttatctt ttacccagcc cgttttcatt tcccctgatg gagcaaagta ataccattgc
     8161 cccttatatt gaagccaacc tgtcttcata gagccatcaa catccaaata ataccactgc
     8221 tctttatctt gaatccaacc aatttccatc tggttttctt tgttaaaata ataccagatt
     8281 ccagcgattt tcttccaatt tttagcagca acaccagagt ctgtcaaata gaaccaacga
     8341 ttgttccatt ttttccattg attctgtaat aagatacccg tttggttaaa gtaataccat
     8401 tcacccttaa tttcattcca accgacagca tattctcctg tagaatcagg cgcttgatac
     8461 caccagtttc catatgaact cttatgccaa ccagcttgaa aacttggaat cggcttgtag
     8521 gaagttgaaa tgtttactaa accgtcttgt cgaatatcaa aaactgtcgc atcatagtct
     8581 ttgctggctg cgttgattct ctcaattcct cgttctttga gccaattaac atactcacta
     8641 tcaacaccat ttttccaagg tagactatcc gaagtttgaa caatcaaact cggactcaaa
     8701 tttttaatga aatccttggt atttgatttg ttggtatcat ggtgatgatt aaacttcatc
     8761 aaatcaactt ttccaatgag aggaccatac ttgtcttctg ctccatgaac attatctaaa
     8821 tcgcccccaa ggtaaatttt cttgccattg actttcacca cgctaatcaa ggaattggaa
     8881 ttgtcatccc aaattttctt taattcaccc gatgaatcag tttcattttc ataattatag
     8941 agctgaatat ccatgtcccc aaactgaaaa tgagcatccc cttgtgtgat attttgaata
     9001 actgaaacac ctttttctgc agcagtctgt aaaaccttat catagccata cagattatcc
     9061 catagacgtt cagaattagt aatacgatta tcactatatt ttttaagata gactcggtca
     9121 actggatagg tagacagtaa ttcatcaaca tttccaatat gatcactgtg ggtatgggtc
     9181 accaaaataa aatcaagttt ttggacaccc aattccttca aacgacgaaa gacacggtct
     9241 gttagaacat gcttataaga cgtttcaatt ccttctctcc atggatagcg agaatcactt
     9301 ccatctggga aatcataatc ttctcctgta tccaccatgg caaaatgtcc attgctttca
     9361 agaataatcg catcactgcc accttcttga acattgataa agtggatttt atttcctgaa
     9421 ctttcttgag cctgaacatt cccataccat gacaaaccta aaaaagcgcc tacaagtgct
     9481 aaactagtta atttcttttt cattcttact ttctcaatct ctccaaggtt tccttaaaaa
     9541 tctctgggat atctgcttta aattccaagg tcttacctgt tctcggatga gtaaaaccta
     9601 aagtcttggc atgaagaaat tgtccatgtc ctttcaaagt cttgcgagga ccatagacct
     9661 catcaccagc gactggatgg ccgatataag ccatgtggac acggatttga tgagtgcgcc
     9721 ctgtctccag ttgcaactct actaagctat aatcgccaaa gcgttccaag acgtgaaaac
     9781 gcgtcactgc aggcttccct ttagcagtta cagcctgttt cttacggtct ttttcactcc
     9841 ggccaatcgg cgcttcaatt acaccacgat cattaggcag atttccatga acaatcgccc
     9901 aatatttgcg gagagacttt ttatccttga gttcttgggc aagtgctaga tgcgcatcat
     9961 cgtttttagc aatcatgaga agacctgacg tatccttatc aatacggtga acaattcccg
    10021 gacgcagaac cccattgata ccagacaagt ccttaatatg atacatgagg gcattgacta
    10081 gagttccact ggtatgacca gcactcggat gcacaaccat tccctgaggt ttgttgacaa
    10141 cagctacatc ctcatcttgg tagactattt ctagcggaag atcctcagcc acatactcta
    10201 atacctctgg ttctggcaca tggtaagtga cgacatcacc ctcttggact gtgtatttag
    10261 ctttcttgac ttgaccattg accaagacct ggcctgattt aatttgttca ttcgcgagac
    10321 tacgtgataa ttctgacaaa tctgacaaag ccttatccaa acgcagacca ccagtttcaa
    10381 ttttaatttc catttatttc ctcttttagc attgcaatca ataaaataat cactccaacc
    10441 gtcagatagc tatctgccac attgaaaatt gcaaagttga taaagtcaag gtggaacata
    10501 tccacaacaa agccctgact ggccctgtca ataaagtttc caagaccacc cgcgattatc
    10561 aaagtcaaac ccaagaccat ccagaatgag tcctccatgt gtttatgtaa ataccaaatg
    10621 gcacctatca cgacaaccag agtaatgaca gcgaataaca gctgctgatc ttgtaagata
    10681 gaaaaggctg cacctcgatt ttgcaggtag gtcaagctaa cgaaattggg aatccaggag
    10741 cgcacttcac ccagtggaat ctgctggacg atataggatt tgaccaactg atccagccca
    10801 attaaaagca gtacaatgac tgccactatt gctctttttt tcatgatttc ctcttttgat
    10861 caaaatattc ttgcatgact tctacgaaga gagtcccagc ttgactaagc tccacttctt
    10921 cacgtttaac atagaccatg cggttatcta ggttatcctt gagacgaata actgtaatac
    10981 cattaacact gtcactatct aaaaatccag aacctgtcgc ataggcgtcc gtccgctcca
    11041 aaataccatt caaggtggca cggtctgtca cattaaacat ctgtgagcta gcgctggtat
    11101 cgacaaagtt ctctgaataa taaaggtact cgtctttctc ttgagtgaaa cgaaccgttg
    11161 gtaaatccgc taaatcctcc atgactaatt cctctttctg ggctaaagga tgaccctcac
    11221 ggagataaat atgggtatgg aaaggaatca attcgatgac ctccagacct aatttttcaa
    11281 cccgttgcat aatccccttt ttattttgat tgttgaggta gataatccca atctcactat
    11341 gcccttgcgc cacttcatct aatatttgaa cagtagttga ttcaaaaata cggaagttct
    11401 tatagtcagg atagcgctct gaaaaggccg taatagttgg tggcaagaag tcatagtgct
    11461 ggctagcaac agaaaattca tctttttctt cttcaggatt ggcatactga ttttgaaaaa
    11521 tatcaaatcc tttaaccaat tcttgcgatt tttcatagaa ttccatccca cgacgggtca
    11581 agaaagtccc tgagctggtc cgacggaaaa tcttaaaacc caactctttt tccaaatcac
    11641 gaacagaaat agacagactc ggctgactaa catacatctt ttcagcagct tcacggaaag
    11701 taccactatt ggcaatggca acaacatagc gtaattgttg aatgttcatc ttctacccct
    11761 aacttcttta ttttttcatt ataccatatt ttagaagttt tccaaaaagg aaaaaagaac
    11821 atcctattct tcttcactat ctgcctttct caagccaacc ttattttcaa aatcaattca
    11881 aaaaaataag tggctacaca ccacttcagt atcggaaaga aaaacgttga ctatttgtga
    11941 aaaaagaaaa tgccggaaag ttccgacatt attttagtaa gctaacttcc tgaaaatagg
    12001 agcaaccaca cgtcccagaa ggcagtgata ttaatgagaa tatgaactag tattggatag
    12061 tagagatttt tagtcatgcg atacaataga gctaaaataa gacctccact agcataaatg
    12121 aaaaaagcaa gaggagttaa agcaaaattg atatgaatat aaccgaaaat aatagcagaa
    12181 agcaaaacat ctccgtacca aggtgagttt ttgaaaaagg ttgtcataag cacacctcga
    12241 taaatcaatt cctcagcaat aggggcgatg aagcaaacga tgagcaagaa ataagggaac
    12301 tcctgtctcc ccatcatttc taccgtttca ttcaaagaaa tctgatttga agacagggat
    12361 atgaaatacg aaaagaggaa gtcagacata tatgaaatga tgtagcccag taaaaggtag
    12421 atgaagtacc tcagctgcca cttgaaatga aaaattttct ttcctgcaaa aaccagtaga
    12481 tagatgaccg ctaagaaaag aactccgctc tccatcaaaa gcagaatctg aaaaaattca
    12541 cgacttgctg gtagataggg ctttgcgagg tgattgaaaa ctctccaaaa ccaagttgat
    12601 ttgtaaaaaa ttaaggacaa tgctagtaaa atttgaatag cccgtttttt catattaaat
    12661 cctctgtttt ctccccttgt ttcttcatat tcctaataaa ttttacttaa aatctcgcag
    12721 cacttccttt gcaaagatga ttacctcctc caatatatcc taaatcatcg gttcctctgg
    12781 gacaaaatcc atgaaccggc atagttaata ctggttccat ttgccaagga taataaccat
    12841 taggttcttt accataggga tctgctccac cttgtatcag ctctaattct tcatttgtta
    12901 gatttatatt ttttgtcatt aaaatcatga tatatttcct ccgttattgt ttagattagt
    12961 tgagtaatct gctactcata cctacttaca aaaaaaacta ttatattaag ttggtttttt
    13021 aatttgtcta gttgcaacat tgacaaacat agtatagcat atctttgcca aatatcctct
    13081 tcaaatcatg aattgtcatc aaaacatctt aaactataaa atcaattagt ctcaagcttt
    13141 ctatcaattt cttctccaaa atatgctata ataatagcaa aagataaaga aggaagacct
    13201 atgattaaac tactagcctt ggatatggac ggcaccctcc tcaatgaagc caaggaaatc
    13261 ccacaagctc acattactgc tattcacaaa gccattgaaa aaggtgtcaa actggttctc
    13321 tgtacgggtc gccccctttt cggtgtcctc ccctactaca aaaaactgga gcttgacctc
    13381 cagaacgaat atattattgt taacaacggt tgttcaactc accagaccag tgattggagt
    13441 ctagttgact ggaaagaact gagtccagct gacatcgaat acctctatga ccttgctgaa
    13501 aagagtgatg ttcagttgac actttttgac gagtcacatt attttgttct cggtggcaaa
    13561 cccaatcaag ttattgaaaa tgatgctaaa ctagtatttt cagacctgac tgaaatttct
    13621 cttgaagaag cgactagtgg aaagttccgg atgttccaag gtatgttttt aggaacaaaa
    13681 gaacaaacag acgattttga gcagcgtttt gctgaagagc tttgccaacg attcagtgga
    13741 gttcgttcgc agcctgtcat ttatgaagca atgccgcttg gaacgacaaa ggctactgct
    13801 ctttcacgac tagctgagat tttgaagatt gattcctcag agattatggc catgggcgat
    13861 gctaataacg atatcgaaat gctccagttt gcagggcttg ggattgcaat gggaaatgcc
    13921 agcgattatg tcaaatctct tgcggatgcc gttacctcaa gcaacgaaga agacggcgtt
    13981 gcgcgtgcta ttgagaaata tattctataa aaaagaaaag caaatagaca gaagttactg
    14041 ctatttgctt ttttgctaat attttaaaat acggactaat caatagacaa gaatagcgaa
    14101 tcataaaaat cgatttacta gatgccatag tatcttatag ttgctaataa gaagttagtc
    14161 tagcagaatg aatctctcat ttctcccaca ctaatctgta atttgtctat acatttctgg
    14221 tcttctatct cgaaacaagc cccagtttag acgttcactt gctcccttgt ctaggtcata
    14281 agtagctaac agaacagctt cttcttgtct ttcagctcgt tctagaatag ctcctgtttc
    14341 atccgtcata aaggaggaac cgtagaagtc aagactggaa ctctgtccgc cattttcctc
    14401 actaggagta acctcctcta aaccataacg attggctgcg atgactggaa caatattcgc
    14461 tgctgcgtgc ccttgcatag tacgttgcca gtgaccacaa ctatctgtat ccaaaattgg
    14521 ctctgaaccg atagctgtag gataaaagag caattcagca ccattcaatg caagacagcg
    14581 ggctgtttca gggaaccatt gatcccaaca gataccgata ccaatcttag catagcgagt
    14641 attccagacc ttgaaaccag tgttaccagg cgtgaaatag aatttttctt gataataatg
    14701 gtcatctggt atatgggtct ttcgataaac gcccagcact cccccatctg catcaatgac
    14761 ggcaatagag ttatacaaga cattaccatc tttttcatag aaactgattg gtaaaacaac
    14821 ttgtagttcc ttagcaatca ccttaaaatg ctgaatggca gtattttccg ctacagattg
    14881 ggcatactgg tagtagtcat actgacgttc ctgacagaaa tagggatgtt caaacaactc
    14941 gggcaagaga ataatttggg ctccttgctc agcagcctga cgtactaaac gctctgcggt
    15001 ttggatattt gttgccacat ccttagcgca ttgcatctga atggttgcaa ctcttacatt
    15061 tctcatcttt ttctcctatt ctggaatttg ttgggtgata cagtggatat tgccaccacc
    15121 taagagaata tctctggctg gtattccgac aactttacgg tctgggaaac acttgctgag
    15181 gatatctaag gccacttggt cgtttacatc ctcaaactgt ggaaccaaga cagccttgtt
    15241 ggcgatataa aagtttacgt aggaagctgc tagtcgttca cctgcgtatc gcttttcttc
    15301 tccttcttca tagatgtagc ctggcaaatc ttcttctgtc acaacttgtc gaactgcagg
    15361 gataggcaat ttatgaatgg tgaagtgaca accttttgca tctgtttcct gttctaagag
    15421 ttcgagatct gcttttgaca tggcatactg gggatcattt tcgtcatctg tccaagccaa
    15481 aacaagctca gcaggaccaa caaaggcagc aacattatcg acgtgttcat tggtttcatc
    15541 ctgataaata ccataaggaa gccaaataac tttttcagca ccaagacttt ctaataatgt
    15601 gttttcaatc tcctctttag tcaagttagg attgcgacca ggactaagca agcaactttc
    15661 agttacgaga atagttcctt gaccatcgct atggattgcg cctccttcca gtacaaaagg
    15721 tttagcatca tagacaggcc tttccaaggc ctcagcaaaa cgactggcta cttggtcatc
    15781 ctcttcataa tcttgataaa gaccatcata ggtgcctccc caagcattga aggcccaatc
    15841 cacggctaat ttcttacctt tatcattgac gagaatggtt gggccagtat cacgcgccca
    15901 ggcatcattg gtgggaatgt ctaaataaac aaccttgtct ccaagatagg attgggcttc
    15961 agatagatag gcctgctcca ccaaaagata gactctttcc ccttctgcta tggtctcgat
    16021 gatctgagta aatgatcttt tagcagcctt tccttgaaaa ggccatgatc ctggtcgagt
    16081 cggccatatc atgagggtac catgatgggg ttcgtactct gctggcatgt gatagcctaa
    16141 tttttttgga ctgtccatca tgataatctc cctttaaagt cttgatagcc aaaagctttg
    16201 agtaagctac agtctccctg ttcgtccatg agatagagac ttggcaatcc aataccatta
    16261 aaggtattat ttttgacaaa agaataaatg gccatgtctt gaaaataaag tctgtctccg
    16321 atttggactg gattttcaaa actataatca ccaatcacat cgcccgtcag acaggtatta
    16381 gaagaaagtc tgtaggtatg ggctttttcc tgtgcctcaa agccatttct caaaggtgga
    16441 cgatagggca tctcaagtac atcaggcata tggcaggtcg cagaggcgtc taaaaccaag
    16501 atttccatac cattttctac aatatctaat acctcagttg ctaaataacc cgcattaagc
    16561 gcaatggctt caccaggctc gatatagatt tcaagattgt aagtttttcg gatacgcttg
    16621 atttctgaaa tcagcaaatc cacatcgtaa ccttctcttg taatatgatg accaccaccc
    16681 atattgagcc attttacctc atgtaagtag ggaccaaact gttcttctac tgctttcaaa
    16741 gttgtttgta aatcatctgc tccctgctcg caaagggtat gaaaatgaag tccgtcaacc
    16801 aaatctagca aatcactcgg aatcttgtct atagtaactc caaagcgaga acctggtgca
    16861 caagggtcat agagcgcgtg atctccttga gttgaacact gagggttgag gcgcaaacca
    16921 acactgacac cagcctctcg acaacgcgaa ccatgcttac gcaactgtct ctctgagtta
    16981 aagacgatat ggtctgttat cccaagcaat tcctccaagt ctgcatcctt gaaagcaggc
    17041 gcaaatacat ggacttcacc aggaaattct tcccttgcca atttggcctc atagagtcca
    17101 ctagctgtcg tacctgatag atactggcta atcaagggat aagttttgta gagggaatat
    17161 gccttctggg caagcaagac cttgcaaccg acctcttctt gtacatattg tagaatgtgg
    17221 caattagctt ctaacttggc caagtcaata acataggctg gtgttggtac ttgttctaac
    17281 ttcattagtc caccatttgt ggattttcaa ccacaaccca tggcaaacca tactcattca
    17341 aagcttccat gaatggatct ggatctaact cctcaaggtt atacactcca gcttgtttcc
    17401 aagttccgtt catgactaat tttgtcccaa tcatggctgg aacgcctgtc gtgtatgaaa
    17461 ttgcttgtga cccgacttct gcatagcact cttgatggtc gcaaacattg tagatgtaga
    17521 tagtcttttc aacgccatct ttgacacctg taaagataca tccaatattg gtttttccga
    17581 ctgtacgtgg gccaaggcta gcaggatctg gaagcaaggc tttcaaaaat tgaattggaa
    17641 caatttcttg gccgttaaag ttaatggtat ccgtacgaag gagtccaaca ttttcaagac
    17701 atttcatgtg cgtcaagtaa gattgaccaa aagtcataaa gaaacgaatg cgtttgacac
    17761 ctggaatgtt cttggccaat gattcgattt cttcatggtg aaggagatac atatcttttt
    17821 gtccaacttg agggaaatca tactcacgct tgatagacat agcttcgact tcgacccatt
    17881 tcccatcttc ccagtaagaa cctggcgcag aaacctcacg gagattaatt tctggattaa
    17941 agttggttgc aaatggataa ccgtggtcac cgccattaca gtctaaaatg tcgatataat
    18001 ggatttcatc aaaatagtgt ttgagggcat aagctgaaaa gacactagtt acacctgggt
    18061 caaagccaga accaagaaga gcagtcaagc ctgcttcttt gaatttctct tgataagccc
    18121 actgccatga gtagtcaaag taggctgtaa aaccaagttc cttacaacgt ttttcgtaga
    18181 tagcacgcca ctcagggtct tctgtgtctt ctgcttcgta gttggctgta tcgatatagt
    18241 gaacacctgt tgccaaacaa gcatccataa tggttaaatc ttgataaggc agagctacat
    18301 tcaaaacagc ttctggtttg tagctttcaa tcagggcaat cacttcttca accttgtcag
    18361 catcaagtgc tgcagtttca attttagtac ttgttttgcc ttctagcttc gctttcaagt
    18421 catcgcattt tgacttggta cggctagcaa tcataatctc tgtaaatgtt tcgctatctt
    18481 gacaaatctt tgaaatagca acttgggcaa cgcccccaca accaataact agtaaacgac
    18541 tcattttttt ccttcctctt cttctaaaat gtcctcaaca tacttgggca acataaagga
    18601 tcccacgtgt aagtttgcag tgtagtattc tgtgaaaagc tggcgttttt tccaaccttc
    18661 cttgtcaaaa tctttgacag ggtggtattt tttcgatgca aatccaaaca accaatagcc
    18721 agctgggcta gttggaatat gggcctgata aacccgactg attggaaagg attgattgac
    18781 cttgcggtgc atgcttcggc aggccgactc atcctcgtca aagaagggac tcccatgctg
    18841 gtaaatcatg atgccgtctt ccttcagagc tcgataacta ttgccgtaga attccttggt
    18901 aaagagtcct tccgtatggc caaatggatc tgtcgcatcg ttgatgataa tatcgtaatc
    18961 atcttcgcag tttcgcaaaa agcgtagccc attttggtag taaatggtaa cacgaggatc
    19021 atctagccct gcagcaaagt ctgggaaata ctcacgacag acctcgacca acatctcatc
    19081 cggttccacg atatcgattt gttccagttc aggatagagt gttaatactt gggcaacacc
    19141 gccgtcacca cccccaataa ccaatacttt ctttggattt gggtggacag ccatgggaac
    19201 gtgaacggtc atttcattgt agacgaaatc atccgcatct gagaacaaga catggccatt
    19261 taaaatcagt attttcccaa aagctggcgt atccaagact tcgatatcct gccattcact
    19321 ttttccagcg taaagttgct tggctgttct cagagacaat ttgacatctg gagtatgaac
    19381 ttcagaaaac cataaatcca tctatttctc cttatattat atttagcgca gttagctact
    19441 gccaaagccc aagtggtata cttggaataa gccactgtgg attagtttat tccctccaat
    19501 cgcttgacga tttccgaaag gctctaacca cagttttttg tttcaattgg taatgagttt
    19561 gataacgcat agacgtttta tctcgtctaa ggtaacaagg caaaaagttc gtggataacc
    19621 aaatagactt gaaatactac atctggaggt actcccatgc tttatgtggg cattgatgtc
    19681 gctaaaaata aacacgatgt tacagccttg aatgttcaag gaaaaactgt tcttaaacca
    19741 ctcacttttt caaataataa agctggtttt gaactcttag atctgtctct tcgacagctc
    19801 aaccaagact gtctcatcgc tcttgaagat actggacatt atgcctttaa tctactgaac
    19861 ttcctgcatg aacaaggata taaagtttac acctacaatc ctttgctcat caagaaattt
    19921 gccaaatcat tatcacttcg caagactaaa acggataaga aagacgctca tggcatcgct
    19981 cttaaacttc tttctgaccc caatcgtgaa caatttcaac acaataatcg gcaagtagaa
    20041 ctaaaaatac tgactagaca tattcatcgt ctcaagaaaa aacagtctga ttggaaagta
    20101 caatacactc gttgtcttga t
//
LOCUS       DAVKQL010000041        18993 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVKQL010000041 DAVKQL010000000
VERSION     DAVKQL010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18993
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.041"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(1..633)
                     /locus_tag="VW561_001421"
                     /pseudo
     CDS             complement(1..633)
                     /locus_tag="VW561_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(736..873)
                     /locus_tag="VW561_001422"
                     /pseudo
     CDS             complement(736..873)
                     /locus_tag="VW561_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            888..1235
                     /locus_tag="VW561_001423"
     CDS             888..1235
                     /locus_tag="VW561_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEU9943669.1"
                     /translation="MLFLFESSIIVENGLGAMDTPDENGYVADDYRITYLEAHIKAMR
                     DAIYQDGVDLLGYTTWSCIDPVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYW
                     YKDVIDSNGASIE"
     gene            1356..2300
                     /gene="manA"
                     /locus_tag="VW561_001424"
     CDS             1356..2300
                     /gene="manA"
                     /locus_tag="VW561_001424"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEU9943670.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLHDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDDLLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTVKADDLRSTLLVSNDFFAVYKWEITGKVDFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGSHFILPSDVEAWTLEGQGLELIVSHP"
     gene            complement(2321..3799)
                     /locus_tag="VW561_001425"
     CDS             complement(2321..3799)
                     /locus_tag="VW561_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEU9943671.1"
                     /translation="MIFLTDLRKHDRIVKSINQTEGYLTTQVAFSYFEKGDQSLTMSE
                     KSQWGSKLGFILASAGSAIGLGSVWKFPYMTAANGGGGFLLIFLISTILIGFPLLLAE
                     FALGRSAGVSAIKTFGKLGKNNKYNFIGWIGAFALFILLSFYSVIGGWILVYLGIEFG
                     KLFQLGGTGDYAQLFTSIISNPAIALVAQAAFILLNIFIVSRGVQKGIERASKVMMPL
                     LFIIFVVIIGRSLSLPNAMEGVLYFLKPDFSKLTSAGLLYALGQSFFALSLGVTVMLT
                     YASYLDKKTNLVQSGISIVAMNISISIMAGLAIFPAMSAFNIQSEGGPSLLFIVLPQL
                     FDKMPFGTIFYVLFLFATVTSSVVMLEINVDNITNQDNSKRAKWSVILGILTFVFGIP
                     SALSYGVMADVHIFGKTFFDAMDFLVSNLLMPFGTLYLSLFTGYIFKKALAMEELHLD
                     ERAWKQGLFQVWLFLLRFFVSSFQSSSLWSSLPNLCNQKGLE"
     gene            3904..4653
                     /locus_tag="VW561_001426"
     CDS             3904..4653
                     /locus_tag="VW561_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU9943672.1"
                     /translation="MYHIKEAAQLSGVSVKTLHHYDKIGLLVPLKSENGYRTYSQEDL
                     ERLQVILYYKYLGFSLEKIAELLKEERTDLLPHLTRQLDYLTRERQHLDTLISTLQKT
                     IQEQKGERKMTIEEKFAGFSYQDNQKYHQEAVEKYGQEVMGQALERQKGHEDEATAAF
                     NQVFQTLAQNLQAGLPATATENQEQAAKLLQAIRTYGFACSIEVFGHIGKGYVYNPEF
                     KENIDKFGSGIAQYTSDAIAAYVQTNAEKIG"
     gene            complement(4673..5128)
                     /locus_tag="VW561_001427"
     CDS             complement(4673..5128)
                     /locus_tag="VW561_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9943673.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWPGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEELSEFFYYKNGDDWLYDLK"
     gene            complement(5155..6330)
                     /locus_tag="VW561_001428"
     CDS             complement(5155..6330)
                     /locus_tag="VW561_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU9943674.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITELEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVLDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(6389..7234)
                     /locus_tag="VW561_001429"
     CDS             complement(6389..7234)
                     /locus_tag="VW561_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9943675.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            7359..7916
                     /locus_tag="VW561_001430"
     CDS             7359..7916
                     /locus_tag="VW561_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU9943676.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            7935..8402
                     /locus_tag="VW561_001431"
     CDS             7935..8402
                     /locus_tag="VW561_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEU9943677.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMNDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            8491..9120
                     /gene="upp"
                     /locus_tag="VW561_001432"
     CDS             8491..9120
                     /gene="upp"
                     /locus_tag="VW561_001432"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEU9943678.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            9294..9884
                     /gene="clpP"
                     /locus_tag="VW561_001433"
     CDS             9294..9884
                     /gene="clpP"
                     /locus_tag="VW561_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEU9943679.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            9963..10211
                     /locus_tag="VW561_001434"
     CDS             9963..10211
                     /locus_tag="VW561_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEU9943680.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            10312..11472
                     /locus_tag="VW561_001435"
     CDS             10312..11472
                     /locus_tag="VW561_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9943681.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            11740..12609
                     /locus_tag="VW561_001436"
     CDS             11740..12609
                     /locus_tag="VW561_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU9943682.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILGISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            12613..13569
                     /locus_tag="VW561_001437"
     CDS             12613..13569
                     /locus_tag="VW561_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU9943683.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILSVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKSPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            13569..14333
                     /locus_tag="VW561_001438"
     CDS             13569..14333
                     /locus_tag="VW561_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9943684.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLDYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            14333..15043
                     /locus_tag="VW561_001439"
     CDS             14333..15043
                     /locus_tag="VW561_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9943685.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRRSSGKIEFLGQEIQKMPAQKIVASGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            15351..16007
                     /locus_tag="VW561_001440"
     CDS             15351..16007
                     /locus_tag="VW561_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9943686.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKISILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            16115..17210
                     /gene="prfB"
                     /locus_tag="VW561_001441"
     CDS             join(16115..16186,16188..17210)
                     /gene="prfB"
                     /locus_tag="VW561_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEU9943687.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            17228..17920
                     /gene="ftsE"
                     /locus_tag="VW561_001442"
     CDS             17228..17920
                     /gene="ftsE"
                     /locus_tag="VW561_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEU9943688.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            17913..18839
                     /locus_tag="VW561_001443"
     CDS             17913..18839
                     /locus_tag="VW561_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9943689.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
BASE COUNT         5818 a         3593 c         4102 g         5480 t
ORIGIN      
        1 ataatcatct ggataagctt gtgaaagctc ttctaaaaag acgttcatcc actcagtatt
       61 acatccacca gctcttaaga aaaatgattc gcctgtatag gcatcaacag ctccataaca
      121 atagcgaaat tctcgtatat agtgactatg gacatgtgga cctactccta ttggagacca
      181 acaagatccc agtttactga ttctaccgaa accagcctca tcttggtaca tcaagcgaac
      241 cttacgaaaa cgtctactgg ttttaaagcg ctttcttgtc ttcttgaatt gagattttat
      301 ttttagacgc aacaatggtt tgagcgtctg ctttcttagg atgttctgga cgtggcataa
      361 tatttcgcca accatggcgc ttcaacagtt gatagaaggc atcacgtgtg taggaacgac
      421 ctaacttctt tttataagcc tgaaataagg catcaattgt aacaaattct cctgcctctg
      481 tagccttcaa atggcgagca agaaaggctt tctcttcctc aactgtcata tatgcatggt
      541 tacgaccacc acgtgtttct tgaaggagag agtcgagtcc gaactcctca tattttttac
      601 atttcgccaa atcgttgttt gattacagtc tatagtgtat tgaatctata acagtacacc
      661 ttagctgcta aaatatttct ataaattagt ttgactttcc tgatagagtt gttcacatct
      721 tatttcaaac cactataaaa gctctataat ctctttataa gatttgccca tcagacgaaa
      781 tagaacgatt tgaaggcgtt tatgatattt agctgtacga gagtctttta aaagtgtttt
      841 gatggttttg atttcttctt tagttgattt catatcactg ttatataatg ctttttttat
      901 ttgagtctag tattatcgta gaaaatggac tcggtgctat ggatacaccg gatgagaatg
      961 gttatgtagc agatgactat cggattactt acttagaggc ccacatcaag gccatgcgag
     1021 atgccattta ccaagacggg gttgacttgc ttggttatac gacttggagc tgtatcgatc
     1081 cggtttcagc tggcacaggg gaaatgaata agcgctatgg ctttatctat gtagatcgag
     1141 ataatgtagg aaatgggact ctcaaacgta gcaagaagaa gtccttctat tggtacaagg
     1201 atgtcattga tagcaatggt gcaagcatcg agtagagcac atttgtttac tatttttata
     1261 aaatcttctc cctgctttat aagatgtaaa aaagagttca aatggaactc tttttttgct
     1321 ataatgaggg gagaaaaatc agacaggaga ttaagatgtc agaaccatta tttttacaat
     1381 cagttatgca agaaaaaatc tggggtggta ccaagctaca tgatgagttt ggctacgaca
     1441 tcccaagtga aaaagtcgga gaatactggg ctatctcagc tcatctaaat ggtgtttcta
     1501 aagttgccaa tggtcgttac gagggaacag atcttgctac tttgtatgcg gaacaccgcg
     1561 aattgtttgg caatcgtcct gagcctgtat ttccgctttt gactaagatt ctggatgcta
     1621 acgactggct cagcgttcaa gttcacccag acgatgctta tggactagag catgaaggcg
     1681 aacttggaaa aacagaatgc tggtacatca tcgctgcgga tgaaggttca gagattatct
     1741 acggtcacaa tgccaagtca aaagaagaac tccgccagca aatcgaggac aagaactggg
     1801 atgacttgtt gaccaaagtc cctgttaagg ctggagattt cttctatgta ccaagcggga
     1861 ctatgcacgc tatcggggcg ggtatcttaa tccttgaaac ccagcagtct agcgatacca
     1921 cctatcgcgt ctatgacttt gaccgcaagg atgacaaagg caacttgcgt gaacttcatc
     1981 ttgaaaaatc catcgatgtc ttgaacattg gtgagccagc aaatagccgt cctgtaactg
     2041 ttaaagcaga tgatttgcgt tccactctcc ttgtatctaa tgatttcttc gcagtttaca
     2101 agtgggaaat tactggaaaa gttgactttg aaaagacagc tgactacagc ttattgagtg
     2161 tcttagctgg tcaaggtcag ctaactgttg acgggaaaaa ttatccaatt caaaaaggca
     2221 gccactttat cctaccaagt gatgttgaag cttggactct ggaagggcaa ggtttggaat
     2281 tgattgttag ccatccataa aaagaaaggg cctgagttca ctactcaagt cctttttgat
     2341 tacataaatt gggcaatgaa gaccacaatg atgatgattg gaatgacgaa acgaagaaac
     2401 gaagaaggaa gagccagact tggaacagtc cttgtttcca tgctctttca tcgagatgga
     2461 gttcctccat tgcaagagcc tttttaaaga tatagcctgt aaaaagtgaa aggtagagag
     2521 ttccaaatgg catgaggaga ttggaaacca agaagtccat agcgtcaaag aaggtcttac
     2581 caaaaatgtg aacatccgcc atgacaccgt aagatagggc tgaaggaatg ccaaagacaa
     2641 aggtcaaaat tcctaaaata acactccatt tagcacgctt gctgttgtcc tgattggtga
     2701 tattgtctac attgatttcc agcatcacga cagaagaagt gacagtcgca aagaggaaga
     2761 ggacgtagaa aatggttcca aaaggcatct tgtcaaagag ttgaggcaag acgataaaga
     2821 gcaggctggg tcccccttca gactggatat tgaaggctga catggctggg aaaatggcta
     2881 gacctgccat gatggatatc gagatattca tggctacgat ggagattcct gactggacta
     2941 gattggtttt cttgtccaag taagaagcat aggtcaacat gactgtaacc cctagtgaga
     3001 gggcaaagaa agattgtccc agagcataga ggagaccagc gctagtcagt tttgaaaagt
     3061 ctggtttgag gaagtaaaga accccttcca tggcatttgg caaactgaga gagcgaccga
     3121 tgataacaac aaagatgata aagagcaggg gcatcatgac tttcgaagct ctttcaatcc
     3181 ctttttgaac cccacgtgat acaataaaga tatttaataa gataaaggct gcttgagcta
     3241 ctagggcaat ggctggattt gaaatgattg aagtaaataa ctgagcataa tcacccgttc
     3301 caccaagttg gaacaatttc ccaaactcaa tacctagata gactagaatc catcctccga
     3361 taacactgta aaaagataag aggataaaga gggcaaaggc gccaatccaa ccgataaagt
     3421 tgtacttgtt attcttgccc agttttccaa aggttttgat agcggaaacg ccagcactac
     3481 ggccaagggc aaactcagcc agcaggagag ggaaaccgat taaaatagtg gaaatgagaa
     3541 agattagtaa aaagcctcca ccgccattag cagcagtcat gtagggaaac ttccaaacgg
     3601 aaccaagccc gatggccgag ccagcagatg ctagaataaa accaagtttc gacccccatt
     3661 gtgatttttc tgacatagtt aaactctgat ctcctttttc aaaataagaa aaagctactt
     3721 gagttgtcaa atagccttct gtttgattga tagacttgac tatcctatca tgttttctaa
     3781 ggtctgtcaa gaaaatcatt ttcaagtttt cacacctttc tcaaaaaagt taaaaaattt
     3841 tctcaaaaac gcttgactct gacctaaggg gaggggttat actatcattg taaggaggaa
     3901 atcatgtacc atataaaaga agctgcgcag ctttcaggtg tctctgtcaa gaccctgcat
     3961 cactatgaca agataggact cttggtccca ttaaagtcgg aaaacggcta tcgaacctat
     4021 agtcaagagg atttggaacg ccttcaggtc attctttact acaaatatct aggcttttct
     4081 ttagagaaaa tagcagagct gttaaaggaa gaaaggacag atttattgcc ccatttgact
     4141 aggcagttgg actatctaac tcgcgaaagg caacatctgg ataccttgat ttccaccttg
     4201 caaaaaacta ttcaagaaca aaaaggagaa agaaaaatga ccattgagga aaaattcgcg
     4261 ggatttagct atcaagacaa tcaaaaatac caccaagaag cggtagagaa atatggtcaa
     4321 gaagtcatgg gacaagcgct tgaacgccaa aaaggtcacg aagacgaggc tacggccgcc
     4381 tttaaccaag tctttcaaac tttggcacaa aatcttcaag ctggtttacc tgcaacagca
     4441 accgaaaacc aagagcaagc agccaagctc ttgcaagcca ttcgcactta tggatttgcc
     4501 tgctctattg aggtattcgg tcatatcggt aaaggttacg tctacaaccc agagtttaag
     4561 gaaaacattg acaagtttgg ttctggaata gcccagtaca cgtcagatgc cattgcagct
     4621 tacgttcaga caaatgcaga aaaaataggc taggaatttc ctagcctatt ttttacttca
     4681 aatcataaag ccagtcgtca ccgtttttgt agtaaaagaa ttcactgagt tcttcttcta
     4741 gaaacacacg aagcatatca gacatatcat cggttgcaag ttttagatga gaaagatttt
     4801 caaagtcctc ccaccaaact ttcccttcgt ctgaagactg gagttcacca gtaaagtgtt
     4861 ctgtcttgta aaaaaggaca acataacgat aatccttgtc gtcataccag tttttgatac
     4921 cacagagttg gggtttggaa atgatcagac cagtttcttc tttcacttca cgaatgacag
     4981 catcgacaaa ggattcgcca cgttcaacat gaccaccagg aaaagtaatg ccaggccagt
     5041 cgggactaac tcggtcttgg accaggacct tatctccgtt tttaatcata cacatgttaa
     5101 caaattcgac tgcctctctt ctgttcattc ttcacaacct ttaatcttta atctttaatc
     5161 ataatgcaga cttcccgcca cccatccggt acagagggca gaagtaatgt taaagccgcc
     5221 cgtgtgggca ttgatatcca gtacctcgcc agcaaagtgg agcccaggaa cgagtttgct
     5281 ttccagagtt ttaggattga tttccttgag actgactcca cccttggtaa caaaggactt
     5341 tgcaagggac atttttccag ttacaggaat tttaagttct ttaatggact ggacaagttg
     5401 ttctcgttcc ttttcagtca gctgtttgac tttttcagga tatccttgta caaaaaattc
     5461 ggccaagcgt tctggtaaca aggtttttaa agcgtttttc aaggattttt cccgattttc
     5521 ttctagaaat gtaaccaagt ccttctcaga aagttgaggc aaaacatcga gtgagagaac
     5581 ctccccacct ttgacaaagc tagacatgcg tagggcagca ggacctgaca aaccaaagtg
     5641 ggtaaagagt aaatcatgag tgatgacatg cttaccataa cttagggtca catcgtccag
     5701 agaaatccct tgtaaggctt tatgtggaaa atctgttaat aaaggacttt cagcagcctc
     5761 aagctcggtg atggtatgct taaaatggcg agcaatctcg tgaccaaaac cagtcgaacc
     5821 agtcgaagga taagacttac cacctgttgt gacaatgagt ttctcacaag tgaaggtttg
     5881 atccgctgac ttaaggacaa actggtcatc tactttttta acagaaacga tttctatttg
     5941 agtagcaact tgaccaccta gttcagtgat tttcttttcc aaagcttcga taatagtccg
     6001 agacttgtca ctggctggaa agacgcgtcc gtggtcttcg accttaagtt taacaccatt
     6061 ttctgtaaaa aagttgatga tgtcatgatt atcgaactgg gagaaaacac tgtaaagaaa
     6121 gcgtccgttt ccaggaattc cagctagcag gttgtctaag ctaccattgt tggtcacatt
     6181 gcaacgtccc ccaccagtcc cagctaattt ttttccaagt ttccgatttt tttcgatgag
     6241 gagggttttc tgtccataaa agctactgga aatcgtagcc atcataccag caggtccccc
     6301 accgatgaca atagtatcaa aatgtttcat agctctattg taccacaaaa aaacaagaga
     6361 tgatggtcac ctcttgtcaa gaatgcaatt aatcaatttc atagcccatc agcaaaccgc
     6421 cctcttctgc atagaaactg cagagaccag aggttggtag aattttaata tccgcttgtg
     6481 ggaaggtttc acggattcgc tctgagagct gttgacaaca tttttcgtta ttgcgttggg
     6541 ccatgacaat acggccacca gcatatccag cttttactaa ctcatcatag gcagcttgaa
     6601 ctgatttctt tgatcccctt gctttttgta gcaattcgag agttccagtt tcactagctt
     6661 ttccgaccat acgaatgttg agaaggccaa cgaccgtacc gataagcttg ctcaaacggc
     6721 cgttcttcac caagttatcg actttggcta ggacaaagag caacttagtt ttttcttgat
     6781 aggcggtgat agcttcaacc acttcttcaa aagacaagcc ctggtcaatc aagtcattca
     6841 atttttctac gagtaggtca acttcaccac cagcagataa actatcaatc acatgaatct
     6901 tagtgtcagg atggtcttcc agataaatat tctttgctag ttgagcacta ttgtgactgc
     6961 cagaaagggt acctgtgatg gttactagga aaatgttttt ggcaccttca aatgctcgca
     7021 aatagtcatc tgggcttgga caagccgatt ttgaagcttc tgcagttgca tacatggttt
     7081 ccatcatttg gtcaatatcg agactggcgt catcaacaaa gacctgatca gctacttgaa
     7141 tggttaaggg gacacttaca aaggttgtgt taatagctgg tgttggcagt tgacgataat
     7201 cacaaccaga gtcagcaata atcttccaag tcatagaaat tctccatctt tgtcagttat
     7261 acattgacaa aggttctgtc tttttttaca attatatcat gaaaaccctt gaaacaaaag
     7321 catcatccgt ctgttgtgac aagtagaaag aaaatgttat gtctgaacgt agaatctctg
     7381 aaaagtctct tgaaaatctc agaaaatcaa accaagaatc caatttatta accagagaag
     7441 ccattgaaac agccctcttg caactcttgg aaaaaaagga gctgaccaag attagtattt
     7501 ctgaattggt caaacgtgca ggtgtttcgc gtgcggcctt ttatcgcaat tatgactcca
     7561 aagaggaaat tttagagagc gtctttaaac gaactgtcca caatattatg gaacagctgc
     7621 atcattacga tttaaagaca gacctttatt tggtttgggt tcaccttttc cgggaggcca
     7681 gaaaggaagc tagagtaatt caattggcct tggattacca tctggaaaaa atctttgtcc
     7741 aagccatgca ggaatttcta gaaaaatacc atgggaaatc aaaaggtgtc agctcttatc
     7801 ttcattcctt ctggagctca gccatcgtct ctgtccttct aaaatggatc aaggatggca
     7861 tgaaggtacc agctgaaaag attgcagatt taggtttacc attttttaaa aaatagagaa
     7921 aaaggagaaa agagatgact gaaaaaagac tggcatggga tgagtatttt gcagcccaag
     7981 ccctactaat tgcgaatcgt tccacttgta aacgtgctaa agtgggcgcg attctggtaa
     8041 aagataataa ggttatttcc actggttaca atggttcggt gtcagggact gagcattgta
     8101 ttgatcacga atgtttggtc attgaaggcc actgtgttcg cacccttcac gctgaggtca
     8161 atgctatcct tcaaggtgct gagcgtggtg ttcccaaagg atttacagcc tatgtaaccc
     8221 attttccttg tctcaactgt acaaaacaat tgcttcaggt cggctgcaag cgcgtggttt
     8281 atatcaacca gtaccgaatg aacgactacg cccaatacct ttatcaagaa aaagggacag
     8341 aattgaccca tttaccactt gagacagtac agacagctct taaagaggct gatctaatgt
     8401 aaaaattatc aaaaataaat ggtttagaaa gatttttaaa ccgttttttg gtataatgag
     8461 aagaataaat tgaaagaagg aattccaaaa atgggaaaaa ttgaagttat taatcaccca
     8521 ctgattcaac acaaattgtc aatcttgcgt cgtacagata cttctacaaa agcttttcgt
     8581 gagctagtag atgagattgc aatgttgatg gggtatgaag tacttcgtga tcttccacta
     8641 gaagatgtgg aaatcgaaac accaattaca aaaacagttc aaaaacaatt ggcaggtaag
     8701 aaattggcca tcgtcccaat cttgcgtgca ggtatcggga tggttgatgg tctcttgagc
     8761 ttggttccag ctgctaaagt tggccacatc ggtatgtacc gtgatgaaga aacacttcaa
     8821 ccagttgaat acttggtgaa attgcctgag gacattgacc aacgtcaaat ttttgtagta
     8881 gacccaatgt tggcaacagg tggctcagca atcttggctg ttgattctct taaaaaacgt
     8941 ggcgcatcaa atatcaaatt tgtctgcctt gtatctgctc cagagggtgt aaaagccctt
     9001 caagaagctc atccagatgt agaaatcttt acagcagcct tggatgaacg tttgaacgaa
     9061 cacggttata tcgttccagg tcttggagat gctggagacc gcttgttcgg tacaaaataa
     9121 gatcgaaaag aagaatgact tggaaaagta tttccagtca cgaaaggagg ttgggttttt
     9181 gtttctgtct aatgaaagca gagcaaaaat ttgacctttt ttgaccaaga tattataata
     9241 gtcttatctt cagtcatttg accaacaaaa ttaaaactca aaaggagaaa tgaatgattc
     9301 ctgtagttat tgaacaaaca agccgtggag aacgttctta cgatatttac tcacgtcttc
     9361 tcaaagaccg catcattatg ctgacaggtc cggttgaaga caatatggct aactctgtta
     9421 ttgcccaatt gcttttcttg gatgcccaag atagtacaaa agatatttac ctttatgtca
     9481 atacaccagg tggttctgtt tcagctggtt tggcaatcgt agataccatg aactttatca
     9541 aggcagatgt ccaaaccatt gttatgggaa tggctgcatc tatgggaaca gtcatcgcat
     9601 caagtggagc aaaaggcaaa cgtttcatgc ttccaaatgc tgaatacatg attcaccaac
     9661 caatgggcgg tacaggtggt ggtacccaac aaactgatat ggctatcgct gcagaacact
     9721 tgctcaaaac tcgtaatacc ttggaaaaaa tcttggctga aaattcaggt cagtcaatgg
     9781 aaaaagtcca tgcagatgca gaacgtgata actggatgag cgcccaggaa acacttgaat
     9841 atggctttat tgatgaaatt atggccaaca attcattgaa ctaatgatga tagaaggcaa
     9901 actcgactgg gtttgctttt tttggtataa tagggagaga tttcttagaa agaggattta
     9961 tcatgtttga aaaagtcaat cgctctggct tgattatcta tctttactat aatcgtgatg
    10021 ccaaaaaact gcaggattat ggagatatta cctatcattc caagaaacat cgttacttac
    10081 aactctatgt tccaactcaa gaagtggagc aattggtcgg acgcttgagc aaggaaaaat
    10141 ttattaaaaa agttagggtt tgtcatatcc aagagttgga aacacccttt gtgggcaatc
    10201 tttatcgata ggaaaacgtt atcatagaaa aagttcaagg aaaacattga caattttctg
    10261 ataattcggt atattcttaa cagactattt aagaaataag gagacaaaaa gatgaagaaa
    10321 aaatttgccc tatcgtttgt ggcgcttgca agtgtagcac ttcttgcagc ctgtggagaa
    10381 gtgaagtctg gagcagtcaa cactgctggt aactcagtag aggaaaagac aattaaaatc
    10441 gggtttaact ttgaagaatc aggttcttta gctgcatacg gaacagctga acaaaaaggt
    10501 gcccaattgg ctgttgatga aatcaatgcc gcaggtggta tcgatggaaa acaaatcgaa
    10561 gtagtcgata aagataataa gtctgaaaca gctgaggctg cttcagttac aactaacctt
    10621 gtaacccaat ctaaagtatc agcagtcgta ggacctgcga catctggtgc gactgcagct
    10681 gcggtagcga acgctacaaa agcaggtgtt ccattgatct caccaagtgc gactcaagat
    10741 ggattgacta aaggtcaaga ttacctcttt attggaactt tccaagatag cttccaagga
    10801 aaaattatct caaactatgt ttctgaaaaa ttaaatgcta agaaagttgt tctttacact
    10861 gacaatgcca gtgactatgc taaagggatt gctaaatctt tccgcgagtc atacaagggt
    10921 gaaatcgttg cagatgaaac tttcgtagca ggtgacacag acttccaagc agcccttaca
    10981 aaaatgaaag ggaaagactt tgatgctatc gttgttcctg gttactataa tgaggctggt
    11041 aagattgtaa accaagcgcg tggcatggga attgacaaac caatcgttgg tggtgatgga
    11101 ttcaacggtg aggagtttgt acaacaagca actgctgaaa aagcatcaaa catctacttt
    11161 atctcaggct tctcaactac tgtagaagtt tcagctaaag ctaaagcctt ccttgacgct
    11221 taccgtgcta agtacaatga agagccttca acatttgcag ccttggctta tgattcagtt
    11281 caccttgtag caaacgcagc aaaaggtgct aaaaattcag gtgaaatcaa ggataacctt
    11341 gctaaaacaa aagattttga aggtgtaact ggtcaaacaa gcttcgatgc agaccacaac
    11401 acagtcaaaa ctgcttacat gatgaccatg aacaatggta aagttgaagc agcagaagtt
    11461 gtaaaaccat aatagaaaaa tgttgaaata gggaatgagc ctttgactca ctccctgttt
    11521 cgatatttaa tactcttcga aaatctcttc aaactgcgtc aacgtcgcct tggattatat
    11581 atgtgactga cttcgtcagt cttatctaca acctcaaagc agtgctttga gcaacctgcg
    11641 gctagtttcc tagtttgctc tttgattttc attgagtata agaacctatc aaaaagtgag
    11701 ggaaaaccct cggaattata aatagaaaga gtgaatctta tgctccaaca actcgtaaat
    11761 ggtttgattc taggtagtgt ttacgcgctg ttagccctag gatataccat ggtttacgga
    11821 attatcaagc tcatcaactt cgcccatggt gatatttata tgatgggagc ctttatcggt
    11881 tatttcttga tcaattcttt ccaaatgaat ttctttgtag cgcttattgt agctatgcta
    11941 gcgacagcta ttcttggtgt cgtgattgag tttcttgctt accgaccttt gcgccactct
    12001 actcgtattg ctgttttgat tacggctatt ggggtttctt tcctattgga gtatggaatg
    12061 gtctatctgg ttggtgccaa tacccgtgcc ttccctcaag cgattcaaac agttcgatat
    12121 gatttgggac caattagctt aacaaatgtg cagttaatga ttttgggcat ttccttgatt
    12181 ttgatgattt tgttacaagt cattgtccaa aagactaaga tggggaaagc catgcgtgca
    12241 gtatcagtag atagcgacgc ggcgcaattg atggggatca atgtaaaccg tacgattagc
    12301 tttaccttcg ctttgggttc tgctcttgcg ggtgcggctg gtgttctgat tgctctttat
    12361 tataactctc ttgagccttt gatgggggtt actccaggtc ttaaatcttt cgttgccgca
    12421 gtacttggtg gtatcggaat tattcctggt gctgctcttg gtggctttgt gattggtcta
    12481 ttggaaacct ttgcgacagc ctttgggatg tcagatttcc gtgatgccat tgtttatgga
    12541 atcttgttgt tgatcttgat tgtccgccca gctggtatcc ttggtaagaa tgtgaaagag
    12601 aaggtgtaaa cgatgaagga aaatttaaaa gttaatattc tatggttact ccttttgtta
    12661 gctggctata gcttgattag tgtactggtt tcagtcggag tacttaatct attctatgta
    12721 cagattttac aacaaattgg aattaatatt attttgtctg ttggtctcaa cttaatcgtt
    12781 ggtttttcag gacaattttc acttggtcat gctggtttca tggcgattgg tgcctatgca
    12841 gcagctatta ttggttctaa atcaccaacc tacggtgcct tctttggagc tatgcttgta
    12901 ggggctttgc tttcaggagc agttgcctta cttgtcggca ttccaacctt gcgcttgaag
    12961 ggggactatc ttgcggtagc aactctaggt gtttctgaaa ttatccgtat ctttatcatc
    13021 aatggtggaa gccttacaaa tggtgcggca ggtatcttag ggattcctaa ctttacaact
    13081 tggcaaatgg tttacttctt tgtcgtgatt acaaccattg caaccttgaa cttcttgcgt
    13141 agcccaattg gccgttcaac cctctctgtt cgtgaagatg aaatcgctgc tgagtcagtt
    13201 ggggttaata cgactaaaat taaaatcatc gcttttgtct ttggtgccat tactgcaagt
    13261 attgctgggt cacttcaggc aggatttatc gggtctgttg taccgaaaga ttacaccttc
    13321 atcaactcaa tcaacgtttt gattattgtt gtatttggtg gactcggttc cattacaggt
    13381 gcgattgttt cggctattgt tctgggaatt ttgaatatgc ttctccaaga tgttgctagt
    13441 gtgcgtatga ttatttacgc tttggccttg gtattggtaa tgattttcag accaggtgga
    13501 ctccttggaa cgtgggaact gagcctatca cgtttcttta aaaaatctaa gaaggaggaa
    13561 caaaactaat ggcattactt gaagtaaaac agttaaccaa acattttggt ggtctaacag
    13621 ctgttggaga tgtgactctt gaattgaacg aaggggaact ggttggatta atcggtccaa
    13681 acggagctgg gaaaaccacc cttttcaacc ttttgaccgg tgtttatgaa ccaagcgagg
    13741 gaacagtaac cctagatggt caccttttga atgggaaatc accttataag attgcctctt
    13801 tgggacttgg acgtactttc caaaatatcc gtctttttaa agatttaaca gttttagaca
    13861 atgttttgat tgcttttgga aaccatcaca aacagcatgt ttttactagt ttcttacgct
    13921 taccagcttt ttacaagagt gaaaaagaat taaaggctaa agctttggaa ttgttgaaaa
    13981 tctttgattt agatggtgat gcagagactc ttgctaaaaa tctttcctac ggacaacaac
    14041 gtcgtttgga aattgttcgt gcccttgcta cggaacctaa aattctcttc ttagatgaac
    14101 cagcagcagg tatgaaccca caggaaacag ccgaattgac tgagttaatt cgtcgtatca
    14161 aagatgagtt taagattaca atcatgttga ttgaacacga tatgaatctg gtcatggaag
    14221 taacagaacg tatctacgta cttgactatg gccgtttaat cgctcaagga actccagacg
    14281 aaattaagac caataaacgc gttatcgaag cttatctagg aggtgaagcc taatgtctat
    14341 gttaaaagtt gaaaatcttt ctgtgcatta cggtatgatc caagcagttc gtgatgtaag
    14401 ctttgaagtt aatgaaggag aagttgtttc ccttatcggt gccaacggtg caggtaagac
    14461 aactattctt cgcaccttgt caggtttggt tcgacgaagt tcaggaaaga ttgaattttt
    14521 aggtcaagaa atccaaaaaa tgccagctca gaaaatcgtg gcaagtggtc tttcacaagt
    14581 tccagaagga cgccacgtct ttcctggctt gactgttatg gaaaatcttg aaatgggagc
    14641 tttcttaaag aaaaatcgtg aagaaaatca agctaacttg aagaaggttt tctcacgctt
    14701 tcctcgtctt gaagaacgga agaaccaaga tgcagccact ctttcagggg gggaacaaca
    14761 aatgcttgcc atgggacgcg ccctcatgtc aacaccaaaa cttcttcttt tagatgaacc
    14821 atcaatggga cttgccccaa tctttatcca agaaattttt gatatcattc aagatattca
    14881 gaagcaagga acaacggtcc tcttgattga acaaaatgcc aataaagcac ttgcaatctc
    14941 tgaccgagga tatgtactgg aaacagggaa aatcgtccta tcaggaacag gaaaagaact
    15001 cgcttcatca gaagaagtca gaaaagcata tctaggtggc taaaacaatc cagtggattg
    15061 ttttagtcgg cagatggaga ttacgaagta atcatcaata tagtccgggg gaccttttta
    15121 gtcggtagat tgagattgca aacaaatctg catctacatt gaaagcttaa tttctaataa
    15181 ttgaaaaaat cgaatgaaaa atttcttacc ttcattcaca gagctcgatt tcagagctct
    15241 ttttgctagc ttattcatac ttttctgaat ttcgaaaaag aaatgtaagc gtttgataga
    15301 tttacaaaaa gattgtataa tagggataag aatagaaaag gagaagtctc atggcagtta
    15361 aagattttat gacccgcaag gtagtttata ttagtccaga tacaacagta tctcatgcag
    15421 cagatttgat gagagagcaa ggtttgcacc gtctgcctgt tatcgaaaat gatcaattag
    15481 ttggtttggt gactgaggga accattgcac aagcaagtcc atctaaagca acaagtcttt
    15541 ctatctatga gatgaattat cttctgaata agacaaaagt aaaagatgtc atgattcgcg
    15601 atgttgtcac tgtctcaggc tatgctagtc tagaagatgc aacttatctg atgttgaaaa
    15661 ataagattag tattctccct gtcgtagata accatcaagt atacggagtt attactgacc
    15721 gtgacgtttt ccaagctttt cttgaaattg caggttatgg cgaagaaggg attcgtgtac
    15781 gctttgttac agaagatgaa gttggtgttc ttggaaaaat tgtttctttg attgtagaag
    15841 aaaatttgaa tatctcccat acagtcaata ttccgcgtaa ggatggtaag gtcattatcg
    15901 aagtgcaaat cgatggatca attgatttac cagccttgaa agaaaaattt gaagcaaatg
    15961 gtattcaagt ggaagaaatc gctcgcactt cagcaaaagt cttgtaagaa gggaagccca
    16021 aaggcttctt ttttcatgaa aaggggatta gagaaaaaga tggaaagaaa tgataaaata
    16081 tgctataatg aaataatgta aaaaaggagt atttatggac atttcagtaa ttcgtcagaa
    16141 aattgacgca aatcgtgaaa aattagcttc tttcaggggg tctctttgac ctcgaagggc
    16201 tagaggaaga gattgccatc ttggaaaaca agatgacaga acctgatttt tggaacgata
    16261 atattgcggc ccaaaaaacg tcgcaagaat taaatgaatt aaaaaacact tacaatacct
    16321 tccataagat ggaagagttg caggatgaag tcgaaatttt attggatttt ttggctgaag
    16381 acgagtcagt gcatgatgaa ctggtagcgc agttagccga acttgataag ataatgacca
    16441 gctacgagat gactctactc ttgtcagaac cttatgacca caacaatgcc atcttggaaa
    16501 tccatccagg ttctggtggt actgaggcgc aggactgggg tgatatgttg cttcgtatgt
    16561 atactcgtta tggtaatgct aaaggcttta aagtggaagt gttggattac caagcaggtg
    16621 atgaggctgg tattaagtcg gtaactttat catttgaagg gcctaatgcc tatggtctcc
    16681 tcaagtcaga aatgggtgtt caccgcttag tgcgaatctc accatttgac tctgccaaac
    16741 gtcgccatac ctctttcaca tctgtagaag tgatgccaga attggatgat actattgaag
    16801 tggaaatccg tgaagatgat atcaagatgg ataccttccg ttcaggtggt gccggtggac
    16861 aaaacgtcaa taaggtttca acaggtgtac gtttaaccca cattccaact ggaattgttg
    16921 tccaatcaac agtggatcgt acccagtatg gaaatagaga tcgtgccatg aagatgttgc
    16981 aggctaagct ctatcaaatg gagcaagaga agaaggctgc agaggtagat tctctcaaag
    17041 gtgagaaaaa ggagatcact tggggaagcc aaatccgttc ttatgtcttc acgccttata
    17101 ctatggtaaa agatcaccga actagctttg aggttgctca ggtagataag gttatggatg
    17161 gggacctaga tggttttatc gatgcttatc tcaagtggcg aattagctaa gatagaaagg
    17221 aactcacatg tcaattattg aaatgagaga tgtcgttaaa aaatacgaca acggaacgac
    17281 tgctctacgc ggtgtttcgg tcagcgttca accgggggaa tttgcttaca tcgtaggacc
    17341 ttcaggagca gggaagtcaa cttttattcg ttctctgtat cgtgaagtaa aaatcgataa
    17401 aggaagccta tcagttgctg gttttaatct ggttaagatc aaaaagaaag atgtcccgct
    17461 tctacgtcgt agtgttgggg ttgtcttcca agattataaa ttgttaccaa agaaaactgt
    17521 ctatgaaaat attgcttacg ctatggaagt aatcggggaa aatcgccgta atatcaaaag
    17581 acgagtgatg gaagttttgg acttggttgg attgaagcat aaggttcgtt ctttcccaaa
    17641 tgaactctca ggtggggagc aacagcggat tgcgattgcg cgtgcaattg taaataatcc
    17701 caaagtattg atagctgatg agccaacagg aaatctggat ccggataatt catgggaaat
    17761 tatgaatctc ttggaacgga ttaacctaca aggaacaact attttgatgg cgactcataa
    17821 tagccagatt gtaaatacct tgcgccaccg tgtcattgcc attgaaaatg gccgtgtcgt
    17881 tcgtgacgaa tcaaaaggag agtatggata cgatgattag tagatttttt cgccatttat
    17941 ttgaagcctt aaaaagtttg aaacgaaatg gttggatgac agtagctgct gtcagttcag
    18001 tcatgattac tttgaccttg gtggcaatat ttgcatctgt tattttcaat acagcgaaac
    18061 tagctacaga tattgaaaat aatgtccgtg tagtagttta tatccgaaag gatgtggaag
    18121 ataatagtca gacaattgaa aaagaaggtc aaactgttac aaataatgac taccacaagg
    18181 tatatgattc tttgaagaac atgtctacgg ttaaaagtgt taccttttca agtaaagaag
    18241 aacaatatga aaaattaacc gagataatgg gagataactg gaaaatcttt gaaggagatg
    18301 ccaatcctct ctatgatgcc tatattgtag aggcaaacac tccaaatgat gtaaaaacta
    18361 tagccgaaga tgctaaaaaa attgaaggtg tctctgaggt tcaagatggc ggtgccaata
    18421 cagaaagact cttcaagtta gcttcattta tccgtgtttg gggactaggg attgccgctt
    18481 tgttaatttt tatcgcagtt ttcttgattt caaataccat tcgtattacc attatttccc
    18541 gcagtcgcga aattcaaatc atgcgcttgg tcggagctaa aaacagttat atccgtggac
    18601 cgttcttgtt agaaggagcc tttatcggtt tattgggagc tatcgcacca tctgttttgg
    18661 tctttattgt ttatcaaatt gtttaccaat ctgtcaacaa atcgttggta gggcaaaatc
    18721 tatccatgat tagtccagat ttatttagtc cgttgatgat tgccctacta tttgtgattg
    18781 gggttttcat tggttcattg ggatcaggaa tatccatgcg ccgattcttg aagatttagg
    18841 taaaatagct gcttttatga ggagattgta aaatctcctt ttttgctaca agagtttttg
    18901 aaaagagatg cgcagaagaa aagagcttcc aaagaagtcc cccagagaag acttcttgat
    18961 ggctaatttg aacttgaaat ttggcgcact aga
//
LOCUS       DAVKQL010000042        18992 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVKQL010000042 DAVKQL010000000
VERSION     DAVKQL010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18992
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.042"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(<1..374)
                     /locus_tag="VW561_001444"
     CDS             complement(<1..374)
                     /locus_tag="VW561_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU9943690.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRYQAGQDKKESNRVAYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGG"
     gene            complement(532..2982)
                     /gene="phtA"
                     /locus_tag="VW561_001445"
     CDS             complement(532..2982)
                     /gene="phtA"
                     /locus_tag="VW561_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HEU9943691.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQTSQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGDHYHF
                     IPYSQMSELEERIARIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPGPQPAPNLKIDSN
                     SSLVSQLVRKVGEGYVFEEKGISRYVFAKDLPSETVKNLESKLSKQESVSHTLTAKKE
                     NVAPRDQEFYDKAYNLLTEAHKALFENKGRNSDFQALDKLLERLNDESTNKEKLVDDL
                     LAFLAPITHPERLGKPNSQIEYTEDEVRIAQLADKYTTSDGYIFDEHDIISDEGDAYV
                     TPHMGHSHWIGKDSLSDKEKVAAQAYTKEKGILPPSPDADVKANPTGDSAAAIYNRVK
                     GEKRIPLVRLPYMVEHTVEVKNGNLIIPHKDHYHNIKFAWFDDHTYKAPNGYTLEDLF
                     ATIKYYVEHPDERPHSNDGWGNASEHVLGKKDHSEDPNKNFKADEEPVEETPAEPEVP
                     QVETEKVEAQLKEAEVLLAKVTDSSLKANATETLAGLRNNLTLQIMDNNSIMAEAEKL
                     LALLKGSNPSSVSKEKIN"
     gene            complement(3236..4969)
                     /gene="ptsP"
                     /locus_tag="VW561_001446"
     CDS             complement(3236..4969)
                     /gene="ptsP"
                     /locus_tag="VW561_001446"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEU9943692.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            complement(4975..5238)
                     /locus_tag="VW561_001447"
     CDS             complement(4975..5238)
                     /locus_tag="VW561_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEU9943693.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            5589..5807
                     /gene="nrdH"
                     /locus_tag="VW561_001448"
     CDS             5589..5807
                     /gene="nrdH"
                     /locus_tag="VW561_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEU9943694.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            5888..8047
                     /gene="nrdE"
                     /locus_tag="VW561_001449"
     CDS             5888..8047
                     /gene="nrdE"
                     /locus_tag="VW561_001449"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEU9943695.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            8235..9197
                     /gene="nrdF"
                     /locus_tag="VW561_001450"
     CDS             8235..9197
                     /gene="nrdF"
                     /locus_tag="VW561_001450"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEU9943696.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            9379..10140
                     /locus_tag="VW561_001451"
     CDS             9379..10140
                     /locus_tag="VW561_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9943697.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNISNLDTIVSDSKLSDSILFKLSKHIKVIKP"
     gene            complement(10419..10559)
                     /locus_tag="VW561_001452"
     CDS             complement(10419..10559)
                     /locus_tag="VW561_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEU9943698.1"
                     /translation="MNFEKCSQIPCLTSEELKSLGKWYVSTGKEWDTISFDSDFMPFQ
                     QS"
     gene            complement(10649..12055)
                     /gene="lacG"
                     /locus_tag="VW561_001453"
     CDS             complement(10649..12055)
                     /gene="lacG"
                     /locus_tag="VW561_001453"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEU9943699.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHACAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGIGRR
                     VAPDYVSRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            complement(12270..13961)
                     /locus_tag="VW561_001454"
     CDS             complement(12270..13961)
                     /locus_tag="VW561_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9943700.1"
                     /translation="MDKLIIFIEKGKPFFEKLSRNIYLRAIKDGFIAGMPVILFSSIF
                     ILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQ
                     INYMSTLLAAIVGLLMLAADPIENGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
                     TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARHFVGASVAESIGKFFAPLFSA
                     ADGYLGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLV
                     LNPIFFIPFIFAPIANVWVFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFI
                     LAALLIVVDVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAG
                     VDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLPE
                     FDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQEQFD"
     gene            complement(13963..14280)
                     /locus_tag="VW561_001455"
     CDS             complement(13963..14280)
                     /locus_tag="VW561_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9943701.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            complement(14316..15152)
                     /locus_tag="VW561_001456"
     CDS             complement(14316..15152)
                     /locus_tag="VW561_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9943702.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEKVIVIGKGIAFGKKKGDLIAENH
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            complement(15386..16366)
                     /gene="lacD"
                     /locus_tag="VW561_001457"
     CDS             complement(15386..16366)
                     /gene="lacD"
                     /locus_tag="VW561_001457"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEU9943703.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQYQTEEP
                     TVAQMEELKILVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAVFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            complement(16368..17297)
                     /locus_tag="VW561_001458"
     CDS             complement(16368..17297)
                     /locus_tag="VW561_001458"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEU9943704.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDDQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKLVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLEELKEVLQESLFTGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAGLLIKA
                     NVLGMLNAQEKMTGHVNMANYQALYDQLIVKEV"
     gene            complement(17308..17823)
                     /gene="lacB"
                     /locus_tag="VW561_001459"
     CDS             complement(17308..17823)
                     /gene="lacB"
                     /locus_tag="VW561_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEU9943705.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            complement(17854..18279)
                     /gene="lacA"
                     /locus_tag="VW561_001460"
     CDS             complement(17854..18279)
                     /gene="lacA"
                     /locus_tag="VW561_001460"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEU9943706.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            complement(18436..18912)
                     /locus_tag="VW561_001461"
     CDS             complement(18436..18912)
                     /locus_tag="VW561_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEU9943707.1"
                     /translation="MPYFIGGHPGFNCPLLDDGVYEDYYLESEKEETCSVPRPFPETG
                     MLDFQDRSPWLEGQKEIDLSYDLFSTDAVTLDELQSRTIALRSLKHDKGLKVHFAEFP
                     NLIIWSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
BASE COUNT         5614 a         3943 c         3431 g         6004 t
ORIGIN      
        1 ccacccttga tttcattgac aatgtctgaa tccttcaact gataattcgg atctttcatg
       61 aggagctctt cactgatgat ggcatcataa ggaaccttgc cattatagta atgataatgg
      121 tctccatgag aggtcacata accttgatcc gtaatcttga taacaatttg ttcggcgttg
      181 atcccctccc tcttactgac ttcatctggt gtcaagtttt ctgccttttg accagcctga
      241 tcaccatcta tataagcaac tcgattagac tctttcttat cctgaccagc ttggtaacgt
      301 ccaagctcat aggaacaaac acttagggca aggactgcca ctgaacctgc tagatatttt
      361 ttattgattt tcattctttc ctcttttctt aagtatattt gattcactat aaagtaataa
      421 accggttaat taactagtta atagtttact ctttttaata ttgcttgtca agaattttac
      481 atatatacat aataaaaatg aaagcctctt tatcgagact ttcatttttc attagtttat
      541 tttttcctta cttacagatg aaggattact tccttttaac aacgcaagta atttttctgc
      601 ttctgccatg atactattgt tatccataat ttgaagagtc aaattatttc gtaaaccagc
      661 tagagtttct gttgcattgg ctttcagact agaatccgtt actttcgcaa gcaaaacttc
      721 tgcttctttg agttgggctt ctactttttc agtctctact tgagggactt ctggctcagc
      781 aggtgtttcc tctactggct cttcatccgc tttgaagttc ttatttggat cttcactgtg
      841 gtctttcttg cctaacacat gctcactggc attgccccat ccatcattag aatgtggacg
      901 ttcgtcaggg tgttctacgt agtacttaat cgtcgcaaac aaatcttcca aggtatagcc
      961 atttggagct ttgtatgtgt gatcatcaaa ccaagcaaat ttaatgttat ggtaatgatc
     1021 cttatgagga ataatcaaat taccgttttt aacctcaact gtatgctcaa ccatatatgg
     1081 aagtcgaacg agtggaattc gtttttcccc tttcacacga ttgtaaatag ctgctgcact
     1141 atctccagtt ggatttgctt taacatctgc gtctggagat ggaggtagga tacctttttc
     1201 tttagtatag gcttgagctg caactttttc cttatcagaa aggctgtctt ttccaatcca
     1261 gtgactatgg cccatatgag gcgttacata tgcatctcct tcatcactga ttatatcatg
     1321 ttcatcaaaa atataaccat ctgacgttgt atacttatca gctaattgag caatacgaac
     1381 ttcgtcttca gtatactcaa tttgagaatt tggcttgcca agtcgctctg gatgggtaat
     1441 tggtgctagg aatgccaata aatcatctac caatttttct ttattagtcg attcatcatt
     1501 caagcgttct aataatttgt ctaaggcttg gaaatcagaa ttacgaccct tattttcaaa
     1561 caaggcttta tgagcctcag ttaacagatt atatgcttta tcataaaatt cttggtcacg
     1621 aggagcaaca ttttcttttt tagcagttaa agtgtgtgaa acactctctt gttttgataa
     1681 cttgctttca agatttttaa cagtttcaga tggtaaatct ttcgcaaaga cataacgaga
     1741 gatgcccttt tcttcgaata catatccttc cccaactttt cgtaccagct gactaaccaa
     1801 agaagaattt gagtctattt taagatttgg tgcaggttgc gggcctggac taggttccgg
     1861 agtcggttgt ggacttggtt gttctggcct tgaatctggt acccaatggt ttgaacgata
     1921 acgaagggga ataatacgag cgattcgttc ttccaattca gacatttgag agtaagggat
     1981 gaagtggtaa tgatctccgt gtggcactgc aacacctcta gctgttcgac ttgtgatttg
     2041 cgctgggtcg aaaacaaggc catcagattc tacatgtcgt tgactcaaag gcagtttgta
     2101 gagctgtttc aagagactat caatgtcatt actttgactt gtttgactgt tagtgttgct
     2161 gttgttgctt gtgttagtat ttgtagttcc tggattgctt acagaaggta cccagtttgt
     2221 tcttgaagtg ttatcgctat tttgtcggcg ataggttctt gaatttgaca gatttccccg
     2281 accagatagg aaggcttctg cagcagccaa ctcgctagct gataactcat tcttaggaat
     2341 gtaatggtaa tgatctccat gaggaacgat ataagcatca ccagtatcct ctatgatatc
     2401 agaagcatta aagatataac catcatctgt agtatagcgt ccttgcgaac gtgccaaggc
     2461 aacagcacca tcgtttcttg gagtcccacc ttcacgatgt tgactatgtt cttgtttttg
     2521 tcgattgatt tcctcttttg tacggacgtt atccgcgtgg gcagcatcct taaggtaaac
     2581 atagtatttt ccatctacct tgataacata tccacccttg acctcattaa caatatcctc
     2641 atcttttagc ttatagttcg gatctttcat gagtaattct tcactgaaaa tcgcatcata
     2701 aggaacctta ccattgtaat aatgatagtg gtcgccatgt gaagtgacat agccttggtc
     2761 tgttatcttg atgacgattt gctcagcatt gattccttca cgcttgctaa cctcatcagg
     2821 agtcaaattc tccgtttttt gcgtcgcttg ttttccatct atataggaaa cacgattatt
     2881 ttccttaacc gttctagctt gatacagtcc caactcgtaa gaacaaacac ttaaaatcaa
     2941 agctgccgca gaaccaacaa ggtatttctt attaattttc atcgaaactc tctttctatt
     3001 tgatatttct agttgcaatt catttctaaa ttgcagaaac tcctctctat tacttaactg
     3061 gttaatagtt tactcctaaa tctccaactt gtcaagaatt ttatgaacca tttaagtatt
     3121 ttttgttctg actactactt tttcaaggtt ggactttcta taagaaagat tttcttgaaa
     3181 attcttttta aaatatcaaa aaaatccctg taactgagtt gcagggactt ttcgattaat
     3241 caaaattaac gtattctttt tgaagttcaa gaacttcttc cattgttgag cattctgtaa
     3301 gggcacggtt tgcgtactct tccatcttag ctgtgtcgag tttcttcatc aagctgcgtg
     3361 tacgaagtac agatgttgct gacatagaga actcatccaa gcccattccg acaagaagtg
     3421 gaacagcttg ttggtcacca gccatctcac cacacatacc agcccattta ccttcagcgt
     3481 gagctgcttt gatcacattg ttaatcaagc gtaggattga tgggttgtat ggttggtaaa
     3541 ggtatgaaac ttgttcgttc atacggtctg ctgccattgt atattggatc aagtcgtttg
     3601 taccaattga gaagaagtca acttctttag caaattggtc tgcaagcata gccgctgcag
     3661 gaatctcgat catgatacca acttggatgt tatccgcaac tgcaacacct tcagcaagaa
     3721 ggtttgcttt ttcttcatca aagactgctt tcgctgcacg gaattctttc aagagcgcaa
     3781 ccattgggaa catgatacgc aattgaccgt gaacagacgc acgaagaaga gcacggattt
     3841 gtgtgcggaa catagcatct ccagtctcag agatagagat acgaagagca cggaatccaa
     3901 ggaatgggtt catttcgtga ggcatatcga agtaaggaag ttccttatct ccaccgatat
     3961 ccattgtacg aacgacaaca ggtttaccgt tcattccttc aagaacagcc ttgtatgctt
     4021 catactgctc atcttcagtt gggaagtctt gagaatccat gtacaagaac tctgtacggt
     4081 aaagtccaac agcttctgca ccgttgttgt taacaccttc aacgtctttt ggagtaccga
     4141 tattagcagc caactcgaag tgtttaccgt cagcagtcac tgtttgagca tctttcaaga
     4201 gcgcccattc agctttttgt ttcgcataag cttcaccagc tgctttaaat tctgccgctt
     4261 gttcatcagt tgggttgata ataacctcac cagtgatccc gttaacagca aggatgtcac
     4321 cgtctttaac gatttcagtg atgttgtttg tacctaatac agcagcaatt tcaagtgtac
     4381 gtgccatgat agctgagtgg cttgtacgtc caccgatgtt ggttacaaaa gcttttacaa
     4441 agtttttgtc caattgagct gtatctgaag gagtcaagtc atgcgcaatc acaatcactt
     4501 cttcattgat agaagctggg tttggcaatt ttttaccaag aaggtttgcc aatacacgtt
     4561 ttgtcacgtc gcggatatcc gctgcgcgtt cttgcatgta tgggttgtct tccatgcctt
     4621 caaagatagt gataaacata tctgtaactt ctttcagacc tgcttctgca ttcactttct
     4681 tcgcacggat agtttccttg atttggctga tcatttctgg gtcagcaaga accattaagt
     4741 gagcatcaaa aacttgagct gcttcttcac cgagcgtacc tactgctttc tcgcgaataa
     4801 cagaaagctc gtcttgtgat gcctgtagag cggcatcaag gcgagcttct tctgcgtttg
     4861 tatcttcgac tgtaatagtc tcaaatgaca aatccggctg aacgagtaga tatgcttttg
     4921 caactgcaac accgtcagat gctgcgattc ctttaagcat ttctgtcatt tcccttatgc
     4981 caatccttct ttttccattg tttctgagat tgcagcgata gcgtcatctg catctgcacc
     5041 ttcagctgag atagttacgt cagcaccttg accaacacca agactcataa cacccataat
     5101 tgatttaagg ttaactgatt tacctttgta ctcaagagtg atatctgaag caaatttgct
     5161 agcagtttgt accaacaatg ttgctggacg tgcgtgaata cctgtttctg ccactacgtg
     5221 gaaatcttta gaagccatag tttgactctc cttttgttct ttcttttttt gagttatatg
     5281 tgataaccct tacaatttag tattatacca tcttctagga tttttttcaa gcattttctg
     5341 gattttcctt caatttcctt tcagataact tgctgaaaat cttctatcct agataacaaa
     5401 acacaggata tagtttcttc aaaaacaatt tataggataa tcattgctat ttcacaatac
     5461 aaacactata tattgtgttt atatatgaaa acaacaaaag aacaccacta tatttagttt
     5521 aaatcattga caaaatttta ttttctgata tactaagata gtattaaatt ttgaagagga
     5581 gttacacaat ggtaaccgtt tattctaaaa acaattgtgt ccaatgtaaa atgaccaagc
     5641 gtttcttgga cagtaataat gtctcttatc gtgaaatcaa tcttgatgag caacctgagt
     5701 acgtcgatca agttaaagag ctcggtttta gcgcagctcc tgttatccaa acaccaactg
     5761 aagtcttttc aggtttccaa ccaggaaaac tgaaacaatt agcataatct tagtacatca
     5821 tccagaagaa actgctttta gggctaactt agaagccttt cttttgtaat tagataaggg
     5881 aaattttatg ggattaaaac atcttgaaga cgtgacttac ttccgtctta ataacgaaat
     5941 taaccgtcct gttaatggac aaatcatgct tcataaagat aaggaagcct tggatgcttt
     6001 ctttaaagaa aatgtagttc caaacactat ggtttttgat tcaatcaaag acaaaatcaa
     6061 ttacctcatt gaacacaact acatcgaaac agcctttata aagaaatacc gtccagagtt
     6121 cttggaagaa ttggctcaat ttatcaaaga ccaaaacttc caattcaagt cattcatggc
     6181 agcttataaa ttttacaatc aatatgcctt aaagactaat gacggtgaat actatcttga
     6241 aaatatggaa gaccgtgtct tcttcaacgc cctttatttc gctgatggga atgaagctgt
     6301 tgcaatcgat attgccaatg aaatcatcca ccaacgctac caacctgcta ctccttcctt
     6361 cttgaatgct ggacgtgctc gtcgtgggga gttggtatct tgtttcctaa tccaagtgac
     6421 ggatgatatg aactctatcg gacgttctat caactcagct cttcaacttt cacgtattgg
     6481 tggtggtgtg ggaattaccc tcagcaacct tcgtgaagct ggtgcaccta tcaaaggcta
     6541 tgaaggagct gcttcaggtg tcgttcctgt catgaagctt tttgaagaca gcttctctta
     6601 ctccaaccaa ttggggcaac gtcaaggtgc cggtgttgtc tacctcaacg tctttcaccc
     6661 agatatcatc gctttccttt caactaagaa agaaaacgct gatgaaaaag tacgtgtcaa
     6721 gactctatca cttggtgttg tagtacctga taaattctac gaattggctc gtaaaaatga
     6781 agaaatgtac ctcttcagcc cttactctgt agaaaaagaa tacggtgtgc cattcaatta
     6841 catcgacatt actgaaaaat acgatgaatt ggtcgcaaat ccaaatattc gcaagacaaa
     6901 aatcaaggcg cgggatttgg aaactgaaat ctctaaattg caacaagagt ctggctatcc
     6961 ttatgtagtc aacatcgata cggctaaccg tgcaaatcct gttgatggaa agattatcat
     7021 gagtaacttg tgttctgaga ttcttcaagt tcaagaacca agcttgatca acgatgctca
     7081 agaattcctt caaatgggaa cagatgtttc atgtaacctg ggttcaacca acgtggttaa
     7141 catgatgact tcacctgatt ttggtcgttc tatccgtgct atggttcgtg cccttacttt
     7201 cgttacagat agttcacata tcgtagctgt accaacgatt gaccatggaa atagccaagc
     7261 ccacaccttt ggtctcggtg ccatgggact gcacagctac cttgcccaac aactcattga
     7321 atatggttca cctgagtctg ttgaatttac aagcatctac tttatgctta tgaactactg
     7381 gaccttggta gagtcaaaca atattgcgcg tgaacgtggt attaccttcc acaactttga
     7441 aaaatcagac tatgctaacg gaagttactt tgacaagtat gtaactggtg aatttgttcc
     7501 aacatcagac cgtgttaaag aactcttcaa aaatgtcttt atccctggtg ttgctgattg
     7561 ggctgaactt cgcgacaagg ttcaagaaga tggtctttac caccaaaatc gccttgctgt
     7621 agcgccaaat ggttctatca gctatatcaa cgacgtttct gcttctatcc acccaattac
     7681 gcaacgtatc gaagaacgcc aagagaagaa aattggtaaa atctactacc ctgctgctgg
     7741 cttgtctaca gaaaccattc cttactacac ttctgcctac gatatggata tgcgtaaagt
     7801 catcgatgtt tacgctgctg cgactgaaca cgtggatcaa ggactttcac tcaccctctt
     7861 catgcgtagt gacattccaa aaggccttta cgaatggaag agggaaaata aacaaacgac
     7921 acgtgacttg tccatccttc gtaactatgc ctttaacaag ggaatcaagt ctatctacta
     7981 cgtccgtacc tttacagatg acggtggaga agtcggcgcc aaccaatgtg aaagctgtgt
     8041 gatttagttt ctatcataga aacactattt ttccctaagc agcactgata aaatcaaatc
     8101 tactatgtca taacataatt tatgtaacat tttaaaagtc gggcttccga ctttttgtgc
     8161 gatactccta tagaattatg gtaaagtaga aataattgtg agttcgcaat actaaacaca
     8221 gaaagagatt ttaaatggaa acttactaca aagccattaa ctggaatgcc atcgaagatg
     8281 tcatcgacaa atcaacttgg gaaaaactga cggagcaatt ttggctcgat acacgtattc
     8341 ccttatcaaa cgacttggat gactggagaa agctatctaa caaagagaaa gacttggtcg
     8401 gaaaagtttt tggtggttta acccttcttg acactatgca atctgaaact ggggttcaag
     8461 cccttcgcgc agacatccgt acaccacatg aggaagctgt tttcaataac atccaattta
     8521 tggaatctgt ccacgctaaa tcttactcat caatcttttc taccttgaat actaaggctg
     8581 agattgaaga aattttcgaa tggaccaata ccaatcctta cctacaaaag aaggctgaga
     8641 ttgtcaacga aatctaccta aacggcagcc cacttgaaaa gaaagttgcc agcgtcttcc
     8701 tcgaaacctt cctcttctac tctggtttct tcactcccct ctactatctc ggtaacaaca
     8761 aactagccaa cgttgcagaa atcattaaat tgattattcg tgacgagtct gttcacggaa
     8821 cctacattgg ttacaaattc caacttggtt tcaatgaatt gcctgaagaa gagcaagaaa
     8881 aactcaaaga atggatgtac gacctactct atactcttta tgaaaatgaa gaaggctaca
     8941 cagagagtct ttatgacggt gttggttgga ctgaagaggt taaaaccttc cttcgctaca
     9001 atgccaataa agctctcatg aacatgggac aagatccact tttcccagat tcagctgaag
     9061 atgtcaaccc aatcgttatg aacggtattt caacaggaac ttccaaccat gacttcttct
     9121 ctcaagtcgg aaatggttac cttcttggcg aagttgaagc catgcaagac gatgactata
     9181 actacggttt agactaattc tccatcactc tcaatcatca aaaagtaaac tagaaacatc
     9241 aaagttataa cccaacaaaa atatcgaaag ttttgaaaac aaacttcgat atttttgttt
     9301 ttgtttcatt ttgttgtttt tgtatttgaa tgatgaattt gtttatggta agatagtagt
     9361 aggaaacgag gtgataatat gctcaaacaa gaaaaattaa ccaaaatttt agagatagta
     9421 aatagtaaag gaaccataac agttaaacag attatggacg aaatagccgt ttcagatatg
     9481 actgcaaggc gctatttaca ggaattagct gataaagatt tgctgattcg tgtgcatggt
     9541 ggagctgaaa aacttcgaac caactccctt ttgactaatg agcgatcaaa tattgaaaaa
     9601 caagccctcc aaacggcaga aaaacaagaa atagcccatt ttgcaggcag tctagtagaa
     9661 gaaagagaaa ctattttcat tggaccagga acaacattag agttttttgc gcgtgagttg
     9721 cctattgaca atatccgcgt cgtaaccaac agtctacctg tttttctgat tttaagcgaa
     9781 cgaaaattaa cagatttgat tttgataggt ggaaattatc gcgatattac aggtgctttt
     9841 gttggcacat tgaccctaca aaatctctct aatctccaat tttctaaagc tttcgttagc
     9901 tgtaatggta ttcaaaacgg agctctagct acttttagcg aggaagaggg agaggctcaa
     9961 cgcatcgctt taaataattc taataaaaaa tatttactcg cagatcatag taagttcaat
    10021 aagtttgatt tttatacttt ttataatata tcaaatcttg atactattgt ttcagattct
    10081 aaactaagtg attcaatcct ttttaagcta tctaaacaca ttaaagtcat caagccttaa
    10141 taaactgaaa gagcctgggt aacaactcaa tttcagaaga aaaaaagtaa atcttcccac
    10201 aataaaacgc atagtatcag gtgtttcaac acctgatact atgcgttttt ctatatcaaa
    10261 gactttttgc ccaattcctc aaagctatgc attgagaaat ctgcgactcg cttcctagtt
    10321 taaagttgct ctttgatttt cattgagtat aagcaacgtg attagataag agttttacag
    10381 agtactaaat ataagattta acttttttga gaaattggtt acgattgttg aaacggcata
    10441 aaatctgaat caaaggagat agtatcccat tctttaccag tcgagacata ccattttcca
    10501 agacttttca attcttcact cgtcaaacac ggtatttgag agcatttttc gaaatttata
    10561 atataaactt tttttcataa atagattgga taaaatcttt gaacatcttt ttgactcact
    10621 aaaattctat atctaatgac taattctact agtctataat ctgagtttcc gctactttct
    10681 tgtaccagtg agctgatttc ttaggataac gttcttgagt ttcaaaatct acgtagaaga
    10741 gaccataacg tttctcataa ccgtttgacc atgagaagac atccattaat gaccaaatga
    10801 agtaaccttt tacattagct ccatctgcaa tcgcatcaga caagatctcc aagtgttgtt
    10861 tcacgtaatc aatacgacca tcatcgtaaa cagtgttatc aacgaactca tctttatagc
    10921 cgagaccatt ttcagtgatg taaattttct tgtagttagg ataatctttc ttcacacgca
    10981 tgatttggtc atacaaacct tgagggtaga taatccaatc ccaatccgtg cgtgatacat
    11041 agtcaggagc tacacgacga ccaatacctt tgatttgata cttagagctt cctttttcac
    11101 ctttaccatt atggataatt tcagtttctc catcaaaggc ttccatccag tcactcatat
    11161 agtagttgat tcctaggaaa tcattcaagt cttttgcagc ttctaatgct gtaaaatctt
    11221 cttcacgaag atccaaacta ccaccattga ctgataagat atggttgaca ccttccatgg
    11281 tttcagctga atagcgacct agataagttg cgtctaagat gaatttattg tggatgatat
    11341 cttccaactc tgctgcacga acatctgctg gattttcagg atctagagga tatttagtag
    11401 gcagggcgtg aacaacacca atttcccctt tatagccttt ctctttgtac aattttaccg
    11461 cgcatgcatg agacaccatc atattgtggt gtgattgaaa gactttggca aggtcgtact
    11521 ggatacctgg agggaatttc ccaaccaaat attgaccatc accgattggt ccaatttcat
    11581 tgaaggttgt ccaatagttt acttctggaa attcttcaaa acagaaggca gcgtagtcta
    11641 caaaatgttc gatattttca cggtttaaga agtctccatt tgagtggaga gcttctggcg
    11701 tgtcaaagtg atgaagagtt acaaagggct caacatgacg tttgtgacac tctgcaaata
    11761 aattatgata aaactcaaca cctttagcat ttacttgacc ataaccagtc gggaaaatac
    11821 gtgaccaggc aatagaaatt cgaatgccat tgacaccata ctcttctgct agcttgagat
    11881 caactggata tcgattgtaa aaatcactgg ctggttctgc agtgtaccag tagttatctt
    11941 caagatattt atcccacgct actggtcctt taccatcagt atgtgtagca ccttctgctt
    12001 gataggcagc tgttgcgcca ccaaaaataa agtcttttgg aagtgttttt gtcatttttt
    12061 tcacctattt cttgatttaa ataaagtaaa agtgagagga gaggagtcag tgagccatcc
    12121 ttctccatct cacttcttgt accaagtcac cgaattgtga catgaggagg taaaaacgat
    12181 atctttttac cgacgaatta ataaggcgat taggaaattc gccatagcga tttccgtaac
    12241 gataggagac tgtttcacag ttcctagcct taatcgaatt gttcttgtac gaatgctagg
    12301 gcaccttttc catcacgagt taatttgatg tattgagcgc cttctgtttt cgctagttta
    12361 ataccgagct tatctgtttc tgccttcata tcttcaaagt ttgaagcaac ttgaggagca
    12421 aggataacca aatcaaactc tggtaacatt tcacggtgag caccatagcc gcctgctgct
    12481 gctttcacag ggacattgta ttctgctgct gccttattca aagcatttgc aaggagtcca
    12541 cttgtacctc caccagcaca gagaacgagg acatttgttt cttcagtgat ggtattttgt
    12601 gctgcatcga cacctgcttt ttcaagaata gcatccgctt ttgcagtgtt gaagtttgca
    12661 gcaacttttt ctttcaattc atcattagac ttacctgaac gttcttcttc aagaatttgt
    12721 tcatcataga ccttaaggaa tggatagtaa atgacaacgt caaccacgat tagaagagca
    12781 gcaagaatga atgatagaac ttggaagtta gttccaagaa ctagacctag tggagctggg
    12841 gttgtccatg gtagattagc agtaaatgaa ttcattccaa gtgtttcaat aaagaatttg
    12901 aaaacccata cgtttgcgat aggagcaaaa ataaatggaa tgaagaagat tggatttaat
    12961 acaagtggtg caccaaacaa gattggttca tttacaccga agaaggtagg aactactgaa
    13021 gcacgtccga ttgcacggtt acgttttgat tttgttaacc acataaacat gaatggaaca
    13081 accagtgtcg caccagtacc acccatggta acgataaaca tttgtgtacc agaagtaaga
    13141 atcttgtcag cgtgcatacc ttgttggaga aggttcaagt tgacttcagc atttgcatag
    13201 gtaatcgctg cgatagcagg ttcaacgata gatggaccat gaatcccaac aaaccagaag
    13261 aaggcaaagg caccaaagat aatggtaata ccaaggtagc catcagctgc agagaagagt
    13321 ggtgcaaaga atttaccgat tgattcagct acgcttgcac caacaaaatg acgagctagt
    13381 aaatcaagag cataaagtga aacaactgag agagtgaatg gaatcacatc tttaaagact
    13441 tgtgagatat ttggtggaac ttcgtcaggc atacgaatag tgacgttgtt cttaacacaa
    13501 accttataga tggctacagt aacaaaggca gcaaggaagg ctgaaagcaa accttttgtc
    13561 cccaagaatc cagtagctag accattttcg ataggatcag ctgccaacat caacaaacca
    13621 acaatcgctg ctaacagcgt tgacatatag ttgatttgat tggttttctc catgctccgg
    13681 tttactgagt cggtcaatga cttagctgtt gtaccagcta ccaagagagc cagaataccc
    13741 atagaatagc tataaggttt catcagaaag gctacaactt catcagacca tttaaagccc
    13801 catgaatttg gtacaaaggc aatcaagata aagatacttg agaagagaat aacaggcata
    13861 cctgcaatga aaccatcttt tatcgctctt aagtatatat ttctcgataa tttctcaaag
    13921 aaaggctttc ctttctcaat aaaaattatt aacttatcca ttttatttaa ctcctctctt
    13981 gtaaagttca attaaatggt gcattaggtc ttttaacaag atagttgtca tcaagtgatc
    14041 ttgaccatgc atcatggtca cactatacgc taagtcctca ccagaagctt ccttagtcaa
    14101 tagacttgtt tgcgcgtggt gagcctctgc aatacagcta ccagcttcct ctaccagagc
    14161 gtccgctttt tcaaaatcac cagcttcagc agccttcaag gcttccaata gttttgaacg
    14221 agcatcgcca gcataggcta cgatttcaaa acctaacaat gttacttctt ctctattcat
    14281 gatagaattc tccttatata gttttaaata aatttttatg acaataaacg ttggatatgt
    14341 agaactagat aaactcgttc acttttatac aaatcaagac ccgtatgttg cgtaatcaca
    14401 tcatagatct tggaaccaat ctcgaaggct tttggatagg attgtttaat atgatcttcc
    14461 atatccagaa gtgattggtt atcatctcta gatctgtcta aataatccaa gaaataattc
    14521 aaatggatca taaagcgatc atagaaatgg ttattctttt tagttcgttg aattgcatag
    14581 tccgttaaaa cttcttcaac attcctgaga atttctttcc tcttatcaat cgactccaca
    14641 agttccactt cattttcacc ttcagcatta atgaaatgat aagcaatccg aataatttcg
    14701 tcctcaggaa aatgatctgc tagcttctga cggtaaattt caaaagcttc atttgcgatt
    14761 tgaaaagcga caggatactt agcagaaata tctggcagat tactatcctt gtaccttcct
    14821 tgagttagag cttgataaga acagtaaata tgatctgtca aggttacata gagatactct
    14881 tgaatcggat aatgatattt ctttgatagc ttatcaatga tttcataggt cactgtgata
    14941 aaatcaagcg gaacatcttt gagaagagcc ataaagtttt ctctggactc ttcggtcttc
    15001 atccgaaaga ttttctcaac atgattttca gcaatcaaat cccccttctt ctttccgaat
    15061 gcaatcccct taccaattac aatcaccttt tctcccttat catttctgac aagcgagaca
    15121 ttgtgattca ttggatttag aattcgatac atgtcaacct ccttttatat cttaaaggta
    15181 tataagtaca aaaaatgacc acaaatgaac tagaaaatca gctgatttct aattcacctg
    15241 tgatcatgcc taatattact tagtaacact caccttgtat taacttgtga tttaagtata
    15301 gcacaagtaa gttattttgt aaacctttac atacaacttt ttttaaattt ttttagattc
    15361 atgttcctaa actaggagga ctttcttaca cacgttcttt ccatgaagtc gctgttgttt
    15421 gaagaacttt attgagctca tcaatgtttt caaatccagt tgtgcgaagc cattcgcgag
    15481 ctgctgcttc accatctttg atgtaagctt caactgaccc tgcccatgta gcacgaccac
    15541 aaagaactcc gttaaagttt gcacctgatt catgagcaaa tacaagagta tcttggaaga
    15601 gtttagctga tacaccagca ctcaagtaga tgtatggcaa gttcgttgct tcatcttgtg
    15661 ctttgaagaa gactgctgct tcttcacgtg tgtaaaccac ttcaccctca gcgaagcctt
    15721 caacatattt aatgttaaca ggaacttcaa ctttcaagac atcaatgtta aaacgtgggt
    15781 ctgaaaagac tttcatagca ccgataactt tgtgtggttt tactttagcg tattctacag
    15841 aacctgcgtc cgcaattttt tcatcgtaag caaggatttc aaggaagaat gggatatctt
    15901 cagccacaca ctcagaaccg atgcgttcga tgtaggcttg tttttcttga ttgagttcgt
    15961 ctgagctatc tacatcatag taaagcaaga atttaactgc atctgcacct tcttctttaa
    16021 tacgttttgc agaccaaaca tccaagcagt ctggcaagcg ttttgtgctt gttgtgtcat
    16081 aacctgtttt ttcataagca aggagaagac cagctttttc atcaagagcc ttagttgctg
    16141 gaagtccata ctcagggtca agaagcatag atgaagcgta tttagtcaat tcatctgcta
    16201 ccaagatttt aagttcttcc atttgggcca cagttggttc ttctgtttgg tattgagcca
    16261 tgaggcgttt caaagcacca cgttggtcaa atgcaagagc tgagatgata ccattttcat
    16321 cagaaagttt ttctaagcgt acacgttttt gttctgttaa agccatttta tacctctttt
    16381 actattaatt gatcatatag agcttgatag ttggccatgt tgacatgacc agtcattttt
    16441 tcttgagcat tgagcatacc aaggacattt gccttgatga gtaatcctgc atccgattct
    16501 ttgtgaagaa gtcctgaaga aattcctgcc acagtagagt ctccagatcc aacaggattt
    16561 accacctgaa ttctaggaat atctaccttg tagaaagtgt caccatgttt ggcaaaagta
    16621 ccgttggcac caagtgaaac gataatccat tcaatccctg taaacaaaga ttcttgaagg
    16681 acttctttta attcctccaa atcctcagaa acttctcttc ctagaagctg agacaattcc
    16741 tcattattgg gtttgattac tgtcggttta tggggtgatt caagaaccgc ctgaagtgct
    16801 gcaccagagc agtccaagac tactagcttg ccagcttgat tagcaagctc taccaagctc
    16861 gcatagtaat caactggaag gccagctggc agactacctg agatggctac tacttcaact
    16921 gactccaaga gttttttgaa atgttccaaa aagtcttgac cttcctgttc caatacttca
    16981 ggaccttttt caagaacttc tgtttggttg tcaccgtgga gaatagcgat acagttacga
    17041 gtttctccct gaatagaaaa gaaatctttc tttacttgat catcgatatg ttcaaccaaa
    17101 aactcaccaa gtttgccacc cactaaacca gtagcaagaa cagaatcacc aaattctgaa
    17161 agtactcggg taacattaag tcccttacca ccagccgttt tggttacatc caccacacga
    17221 ttgacagtat caatcttcaa ctcatccaag ggataggaaa tatcaatgga tgggttcatt
    17281 gtgactgtta aaatcatagg tcacctctta gtcgtggtat tctccgcgat cccatttttc
    17341 aaggaattct gtaaagaagt ttgcgtctgt ttgttgagca ttgtgacttt caaggtgttc
    17401 aattttcgca atcaatttct tgttttcttc agatggtttg tattcagcat gaatgaaagc
    17461 ttcgatgata tcacacataa gcaattcacc agtaatctta ccaccaaagc cgataacgtt
    17521 agcgttcaat tgttctttag cataaagggc tgttgtcata tcacgaacca aggcagaacg
    17581 aacacctgga actttattta cagcgttgtt gataccaaca ccagtaccac agatacatac
    17641 cccaagatca gcttggccgc tagttacagc ttcccctact tttttaccaa agattgggta
    17701 gtgagtccgt gtatggtcat atgtaccaaa gtcaatgact tcatatcctt ttgatttcaa
    17761 aaattctgaa accgccattt tttcatctgt tacgatgtgg tcgcatccaa ttgcaattct
    17821 catttttaac ttacctttct tactgtaatc taattagccc attttgttca acatgtcaac
    17881 ccggatttga tgacgtccac cgtcgtattt accattaaca aatcctttag cgatgttttt
    17941 agccaattca tcaccaacaa gttgtgcccc catagtgatc atacgtgagt tgttgtggcc
    18001 acgagtcata taagctgaac gttcgtcaga tacttctgca gcaaccattc ctttgatctt
    18061 agttgcaacc ataaatggac cagctccata agcatcaatc acgataccaa ggttttgttc
    18121 ttctttgttt acttctgcag caacagcgag agtcacatca acaaagtctt gaccttcagc
    18181 tgtaacatcc acaaggtgga agttttcttt ttctaagaag tctttcacaa cttctttcaa
    18241 tctcaaacct gcagcatctg caccgataac aatagacata ttgtatactc ctttttattt
    18301 tttctatgct ggattccagc agtgtattgg ctgtctttaa aaaacagtca attagtcctc
    18361 acaaacataa tatactacta ttgtttgaag aagtcaatag tttgtgtttg tttttgttat
    18421 ttatttttta cttttttata aaacttctat ctcaaaccct aattcttcaa cctgattggc
    18481 ttctaagaga caaacattct tcttatcttc taaatgatct ccttcttcaa ggaatgttga
    18541 caagccagac catggttcaa aggtaatgaa aggtcccttg ttcaaagttg accagatgat
    18601 gaggtttgga aattctgcaa aatgcacttt caatccctta tcatgtttaa gagaacgaag
    18661 ggcaattgtt cgagattgca attcatccaa agtcactgca tctgtgctga aaagatcata
    18721 actaagatct atttcttttt gtccctctag ccatggactt ctatcttgga aatccaacat
    18781 acctgtttct gggaaaggac gtggaacaga gcaagtctct tctttctcag actctagata
    18841 ataatcttca tagactccat catcaagtag aggacaatta aatcctggat gtccaccaat
    18901 aaagtaaggc atgaccttgc tagtttcttt attagacttc ctgcaaaact agaatcctag
    18961 ttcatgattg ataataccag cactcaaatt ca
//
LOCUS       DAVKQL010000043        18583 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVKQL010000043 DAVKQL010000000
VERSION     DAVKQL010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18583
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.043"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(154..963)
                     /locus_tag="VW561_001462"
     CDS             complement(154..963)
                     /locus_tag="VW561_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9943708.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGSIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
     gene            complement(965..1768)
                     /locus_tag="VW561_001463"
     CDS             complement(965..1768)
                     /locus_tag="VW561_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9943709.1"
                     /translation="MKQLTIEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLTFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            complement(1778..3265)
                     /gene="tacF"
                     /locus_tag="VW561_001464"
     CDS             complement(1778..3265)
                     /gene="tacF"
                     /locus_tag="VW561_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEU9943710.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SETSLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            3601..4308
                     /locus_tag="VW561_001465"
     CDS             3601..4308
                     /locus_tag="VW561_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEU9943711.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            4313..5335
                     /locus_tag="VW561_001466"
     CDS             4313..5335
                     /locus_tag="VW561_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU9943712.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVIGRHWEKLELFSFAKECYITDNI
                     PEDLAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFENAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            5352..6221
                     /gene="licA"
                     /locus_tag="VW561_001467"
     CDS             5352..6221
                     /gene="licA"
                     /locus_tag="VW561_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEU9943713.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGADFGYYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            6211..7089
                     /locus_tag="VW561_001468"
     CDS             6211..7089
                     /locus_tag="VW561_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU9943714.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAATHD
                     FLSIFILLAFLLVKEGKVRVSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFVAVANGQL
                     LGLMIVFVAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAALFLGTSLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            7101..7790
                     /locus_tag="VW561_001469"
     CDS             7101..7790
                     /locus_tag="VW561_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEU9943715.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYVSGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            7887..8735
                     /gene="dprA"
                     /locus_tag="VW561_001470"
     CDS             7887..8735
                     /gene="dprA"
                     /locus_tag="VW561_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEU9943716.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            9619..10662
                     /locus_tag="VW561_001471"
     CDS             9619..10662
                     /locus_tag="VW561_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEU9943717.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGMGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            10829..12916
                     /gene="topA"
                     /locus_tag="VW561_001472"
     CDS             10829..12916
                     /gene="topA"
                     /locus_tag="VW561_001472"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEU9943718.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            13022..13381
                     /locus_tag="VW561_001473"
     CDS             13022..13381
                     /locus_tag="VW561_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEU9943719.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            13474..14106
                     /locus_tag="VW561_001474"
     CDS             13474..14106
                     /locus_tag="VW561_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEU9943720.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDICLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLDNRQTFIDQVGVTQLHGTKVVF"
     gene            14150..14806
                     /locus_tag="VW561_001475"
                     /pseudo
     CDS             14150..14806
                     /locus_tag="VW561_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
     gene            14825..15457
                     /locus_tag="VW561_001476"
     CDS             14825..15457
                     /locus_tag="VW561_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEU9943721.1"
                     /translation="MNFQLAKYSLLKKFLENIGFTTLEEYGAIFKYLIENVKTDRQII
                     CSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKIPLGELSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKK"
     gene            15485..15919
                     /locus_tag="VW561_001477"
     CDS             15485..15919
                     /locus_tag="VW561_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEU9943722.1"
                     /translation="MVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEF
                     NATGQGSVEAIFNAIDKFFNQSVRLVSYTINAVTDGIDAQDRVLVTVENRDTETIFNA
                     AGLDFDVLKASAIAYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            15931..16968
                     /gene="leuB"
                     /locus_tag="VW561_001478"
     CDS             15931..16968
                     /gene="leuB"
                     /locus_tag="VW561_001478"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEU9943723.1"
                     /translation="MAKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAGPPLPDETLKASREADAILLVAIGSPQYDGVAVRPEQGLMALRKELNLYAN
                     IRPVKIFDSLKYLSPLKPERISGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEE
                     VERIIRKAFEIARNRRKIVTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSA
                     AMLMITNPAKFDVIVTENLFGDILSDESSVLSGTLGVMPSASHSENGPSLYEPIHGSA
                     PDIAGQGIANPISMILSVVMMLRDSFGRYEDTERIKRAVETSLAAGILTRDIGGQAST
                     KEMMEAIIARL"
     gene            16965..17234
                     /locus_tag="VW561_001479"
     CDS             16965..17234
                     /locus_tag="VW561_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEU9943724.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRAWRIPEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            17238..17597
                     /gene="leuD"
                     /locus_tag="VW561_001480"
     CDS             17238..17597
                     /gene="leuD"
                     /locus_tag="VW561_001480"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEU9943725.1"
                     /translation="MAGSSREYAAWALADYGFKVVIAGSFGDIHYNNELNNGMLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            17759..18329
                     /locus_tag="VW561_001481"
                     /pseudo
     CDS             17759..18329
                     /locus_tag="VW561_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019771122.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
     gene            18423..18488
                     /locus_tag="VW561_001482"
                     /pseudo
     CDS             18423..18488
                     /locus_tag="VW561_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849983.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
BASE COUNT         5820 a         3192 c         3931 g         5640 t
ORIGIN      
        1 aaaaaagtct ttaaaattat aaaacgcatc atatcaaggt ttttcaaaaa acttgatatg
       61 atgcgtttta ttgtgggaat atgtatttca ttttctacta aaattatgtt tttgaataac
      121 ctctatctta gtagtctgta taatccccct caatcagttt ttgcgataag ctttaatgct
      181 atgactatac cactcttgca tttcttttga tggtggtgtc atataatcgc catacatctg
      241 ggttaaaaat tggtcatatt ttttgggaac aggcaacata cggccctcaa actcagttaa
      301 aatcagttct ttaaaggtat caactgggaa gatttctttc atcccttcct taccgatccc
      361 aactcctcct tcatattgag gagtgttggt tacagcattt ttgactagtt gatcaatttt
      421 cttgtaaaag tagcgaggat tgacaaatcg gagagcgtac cagctacata atctaagaaa
      481 atcttttagt ttgctatcac cgtgaactgc tcgtgatttt ttgatataag ctagttgacg
      541 aagagccaca tacttatagc tcttgtcgac aatgctcaag tctgtaaatc gatcaattgg
      601 gaagacatcg atgaaaaggc tggtatcatg acgcttgtac ttaacatggt cttctataac
      661 agtagaagtg tccaaaatcg atgcgaaatt atggaagtac caagaagatg tatcgtagga
      721 aagaaccttg tagcgagggt gattttcttc ttcaataatc ttcagtaaac gctcataatc
      781 ctcacgataa agggaaatat caatatcatc atcccaagga atcatacctt tgtggcggat
      841 ggatccaagc atggttccat aactgagaaa ataaggaata tcatgtttct tacaagtctc
      901 atcaatatag tccagcaggg ctagttgaat ttctttaatt tctttttttt ctaaatattg
      961 catcctaatc ctccaattta taagcgtgaa attcatgact gtagaagcgt ttttcttctg
     1021 gtggtagggt catataatct ccataaaatt gtgtcaaaat agtatcaaat ttttcaggtg
     1081 caggaaggct taaattctca aaggttaaat cgattgtttt atcaaaggta ccacttggga
     1141 agacttcctt ttccttaaat tttgaaggaa taaaagccat atactgccca ttttcacgac
     1201 tatatttttg aatttctttc tcgattttat ttgcaaaata acgaggagaa accggtcgaa
     1261 ggagcaacca aaaggctgtt cgtatccaat cttttaaaag gctatcctta tagacaatat
     1321 ttttatgttt actgaaagac agcagtttga agctttccag tttataacaa gtatcaatga
     1381 ccttaggatc atcaaagcga tctataggga aaatatcgat aaagatacca gactcatagg
     1441 tttttgtatt tcgagtatca ataattttag tcgtactgtc ggttatcttg ataaagttgt
     1501 taaagtagtt cttatcagtt tctaaggata ggagcttata cttgcttttt tccttttgaa
     1561 aaatgttaat aaatcgttgg tagtcttctc taggcatgga cagatcaata tcgtcgtccc
     1621 aagggataaa gccctcatgt cgaaccgccc caatcagagt accgtagtta ataatatagt
     1681 tgatattgtg ctttttacag agagtatcaa tataatccaa aatttctaat tcaatttgtt
     1741 tggcatcttc aatggttagt tgtttcattt taaactccta tgattttttg aatttatttt
     1801 ttaaggctag gacatggttt aaaaattcat agaaaatgct atcttttgtg aagacaagta
     1861 gactaatata agagatagct gataacaaga caatcaaacc agtattaatc aaaaatggca
     1921 aattaatgac catatccaca ggatacacga aattaatcag gaaataaatt gctacaaagg
     1981 aaagtgaaaa gagagagtat cgaacagtat agctaaagat atgtcccaag tggatgagtt
     2041 gttttctatg gatgaaaatg atatagaaaa caagtagaga agtctctgat aacatagttg
     2101 tcagtaagta gtattcggga gccacgatat ggttgaaaaa gaggagacta ttcaagccca
     2161 aattgagtag cccagcaaag actgtataga ctgtgatacg tttttcatag ccattggtaa
     2221 agagaatttg ggaaccaaga atggtatcta aggccaggat aatcgtacga aaagcgaaga
     2281 gagaggtcaa gatgccgcct ccgatatatt tttcactacc gtaaagtagg atggcatttg
     2341 gtcctaaaac catgagtcca aaactcagtg gaatgataaa gaagttaaag attcgactac
     2401 ctctattaac cagagaaaca taggcttctt tgtctccttt ccccagatag taactgagac
     2461 gaggcacact cactccaatt gcacctgtta caaccccagc tataacggtc acaattcgct
     2521 gagctatggt atagtaacta acgttgacat caatccctgt tttaacgagg aagaggcgat
     2581 ctaaaaaagt gaagagcata ttggcattgg caaagactaa catggctgtc agagggagaa
     2641 agagtggttt aaaatcactt aggtgaatct taacaagttt gatgtctctt ttaatccaaa
     2701 aataactaat caggtagtta atcagcgtcg ataaactcat cacaagtgta tagacaacaa
     2761 tatcgtgttc atttttaaca aataagaaaa tagagaccag catcaggata cggatgaagg
     2821 cagttttgta aaagagaaaa ctgtaatttt ccagagcttc attgacccat tcgattgaaa
     2881 aaatctgggc aatgagttga atccccataa caaggtagac ctttttgacg attggattat
     2941 cagtaaagaa gagaggatag gctaggatat agacagcagt ggtcaaaatc gtacaagcga
     3001 tgcacaaata aaaaagacta gaaaaggttc tgttaagatc ttttttgtta tccttgacat
     3061 tactgatagc ccttaaaccg tagttataga caccataagt tgcaaagggc aagaaaaatg
     3121 acaaaatagt gtcgactgag ttgaagtaac catagtcagt tcggtccaag acacgcgcga
     3181 cataggttcc agttaggatg ggaaaaataa tattcaagac acgaattccc atgtaagata
     3241 gagcatttaa ttttatactt ttcattcaat ttacctcgtt tttcattata tcataaagtt
     3301 agctaataag aaatgaaggg cagtaagtca agtaatcact ttgaagtttc aaatcttaag
     3361 ttttaagttt tctttaagga aagtatatta ttctaaagga ctctaaaatt tcgcagccat
     3421 ttattagtaa ttgctacaga attcctagtc attactagaa atggactagt ttctttgaat
     3481 aatagaactg cataattctc ctattctaga aggggaggac cagtatttct tttatgatag
     3541 gactagattg tggtataata gagagaataa gtttttttag taagacaaag gagaaaatag
     3601 atgatttatg caggaattct tgccggtgga actggcacac gcatggggat cagtaacttg
     3661 ccaaaacaat ttttagagct aggtgatcga cctattttga ttcatacaat tgaaaaattt
     3721 gtcttggagc caagtattga aaaaattgta gttggtgttc atggagactg ggtttctcat
     3781 gcagaagatc ttgtagataa atatcttcct ctttataagg aacgtatcat cattacaaag
     3841 ggtggtgctg accgcaatac aagtattaag aaaatcattg aagccattga tgcttatcgt
     3901 ccgcttactc cagaggatat cgttgttacc cacgattctg ttcgtccatt tattacactt
     3961 cgcatgattc aggacaatat ccaacttgcc caaaatcatg acgcagtgga cacagtggta
     4021 gaagcggttg atactatcgt tgaaagtacc aatggtcaat ttattacaga tattccaaat
     4081 cgtgctcacc tttatcaagg acaaacacct caaacattcc gttgcaagga cttcatggac
     4141 ctttatggat ctctttctga tgaagagaag gaaatcttga cagatgcatg taaaatcttt
     4201 gtgatcaaag gaaaagatgt ggccttggcc aaaggtgaat actcaaatct gaagattaca
     4261 accgtaacag atttgaagat tgcaaaaagt atgattgaga aagactagta aaatgattaa
     4321 tcaaatttat caactaacta agcctaagtt tatcaatgtc aaatatcagg aagaggctat
     4381 tgaccaagag aatcatatcc ttatccgtcc caactacatg gctgtctgtc atgcggatca
     4441 gcgttactat cagggaaaac gtgatcccaa gattttgaat aaaaagcttc caatggcaat
     4501 gattcacgag tcatgtggaa ccgtcatttc tgacccgacc ggaacctacg aggttggtca
     4561 aaaagttgtc atgattccca atcagtctcc tatgcagagt gatgaagaat tctatgaaaa
     4621 ctacatgaca gggacccatt tcttgtctag tggatttgat ggctttatga gagagtttgt
     4681 ttctctccct aaagatcgtg tggtggctta tgatgctatt gaagatacgg ttgcagccat
     4741 tacagagttt gtcagtgtgg gcatgcacgc tatgaatcgt ctattgactc ttgctcatag
     4801 caagcgggag cggatcgccg ttattggaga tggaagttta gcttttgtgg ttgccaatat
     4861 tatcaactat actttgccag aagcagagat tgtggttatt ggtcgtcatt gggaaaagtt
     4921 ggaactcttc tcatttgcca aagaatgcta tattacggat aatattcctg aagatttggc
     4981 ctttgaccat gcttttgaat gttgtggtgg tgatggtact ggaccagcta ttaatgactt
     5041 gattcgctac attcgtcctc agggaacgat tctcatgatg ggagttagcg aatataaagt
     5101 caatctcaat actcgcgatg ccttagaaaa gggcttgatt ttggttgggt catctcgttc
     5161 tggtcgcatt gattttgaaa atgctatcca aatgatggaa gtcaagaaat ttgccaatcg
     5221 tcttaaaaat atcctttatc tagaagaacc tgtaagagaa attaaagata ttcatcgtgt
     5281 ctttgcaacc gatttaaaca cagcctttaa aacagtgttt aagtgggaag tataagtact
     5341 ggaggttaat tgtggagaaa atcattaaag aaaaaatttc ttccttactt agtcaagaag
     5401 aggaagtcct cagtgttgaa caactgggtg gaatgaccaa tcaaaactat ttggccaaaa
     5461 caacaaataa gcaatacatt gttaaattct ttggtaaagg gacagaaaag cttatcaatc
     5521 gacaagatga aaagtacaat cttgaactac taaaggattt aggcttagat gtaaaaaatt
     5581 atctttttga tattgaagct ggtatcaaag taaatgagta tatcgaatct gcgattacgc
     5641 ttgattcaac gtcaatcaag accaagttcg acaaaattgc tccaatatta caaactattc
     5701 atacatctgc taaggaatta agaggagaat ttgctccttt tgaagaaatc aaaaaatacg
     5761 aatccttgat tgaagaacaa attccttatg ccaactatga atctgttaga aatgcagtct
     5821 tctccttaga gaaaagactg gctgacttag gtgttgacag aaaatcttgt catatcgatt
     5881 tggtgcctga aaactttatc gaatcacctc aaggacgact ttatttgatt gactgggaat
     5941 attcatcaat gaatgatcca atgtgggatt tggctgccct ctttttagag tctgaattca
     6001 cttcccaaga ggaagaaact ttcttatctc actatgagag tgaccaaaca ccggtttctc
     6061 atgaaaagat tgctatttat aaaattttac aagatactat ttggagtcta tggactgtat
     6121 ataaggaaga gcaaggtgca gattttggtt actatggtgt gaatcgttac caaagagctg
     6181 ttaaaggttt ggcttcttat ggaggttcag atgaaaagta aaaacggagt tccttttggc
     6241 cttctctcag gtattttctg gggcttgggt ctaacggtta gtgcttatat cttttcgatt
     6301 tttacagatt tgtcaccctt tgtggtggct gcaactcatg attttttgag catctttatc
     6361 ttactagctt ttctcttggt aaaagaaggg aaagttcgcg tctcaatttt cttaaatatt
     6421 cgcaatgtca gtgttatcat cggagccttg ctagcaggcc ctatcggtat gcaggccaat
     6481 ctttatgcag ttaagtatat cggaagttct ttagcttcat ctgtatcggc tatttacccc
     6541 gcgatttcag ttctattggc tttcttcttt ttgaagcaca agatttcgaa aaatactgta
     6601 tttgggattg tcttgattat tggagggatt attgcccaga cctataaggt tgaacaggtt
     6661 aattctttct acattgggat tctttgtgct ttggtttgtg ctattgcatg gggaagtgag
     6721 agtgttctta gctcttttgc catggaaagt gaattgagtg aaatcgaagc cctcttaatc
     6781 cgtcaagtaa cttcgttctt gtcctatctt gtgattgtgc tcttctctca tcagtcattt
     6841 gttgcagtag ccaatggaca attgctaggt ctcatgattg tctttgtagc ctttgatatg
     6901 atttcctatt tggcttatta tatcgctatc aatcgcttgc aaccagccaa ggctacaggc
     6961 ttgaacgtga gctatgtagt atggactgtc ttgttcgcag ctcttttctt gggaacatca
     7021 ttagatatgc tgaccattat gacgtcactt gtcgtcattg ctggagttta tattattatt
     7081 aaagaataaa ggagattcgt gtgaaagcca ttatcttagc agcgggattg ggaactcgct
     7141 tgcgtcctat gactgaaaat acccctaaag ccttggttca ggttaatcaa aaacctttga
     7201 ttgaatacca aattgagttt ctcaaagaaa aaggaatcaa tgacatcatc atcattgttg
     7261 gttatcttaa agaacaattc gattacttaa aagaaaaata cggtgttcgc ctcgttttca
     7321 atgataaata cgctgactac aataactttt actctctcta tcttgtaaaa gaagaattgg
     7381 ccaacagcta tgttattgat gctgacaatt atctctttaa aaatatgttc cgcaatgatt
     7441 tgacacgttc gacttatttt agtgtttatc gtgaagattg taccaacgaa tggttcttgg
     7501 tctatggaga tgactacaag gttcaagaca ttattgttga tagcaaggca ggtcgcatcc
     7561 ttagtggtgt atccttctgg gatgctccaa ctgcagaaaa gattgtcagc tttatcgaca
     7621 aggcttatgt aagtggtgaa tttgttgatc tctactggga caatatggtt aaggataata
     7681 tcaaagagct agatgtctat gttgaagaat tagaaggcaa tagcatttat gagatcgata
     7741 gtgtccaaga ctatcgtaaa ttagaagaaa ttcttaaaaa cgaaaattaa agattccaac
     7801 atctgacaaa atagtcggat gtttttttga ttttttacga acttttacga atagatagat
     7861 gagtagaaaa agaaatggag ttatttatga aaatcacaaa ctatgaaatc tataagttaa
     7921 aaaaatcagg tttgaccaat caacagattt tgaaagtgct agaatacggt gaaaatgttg
     7981 atcaggagct tttgttgggt gatattgcag atatctcagg ttgccgaaat ccagctgttt
     8041 ttatggagcg ttattttcag atagacgatg cgcatttgtc gaaagagttt caaaaatttc
     8101 catctttctc tattttagat gactgttatc cttgggattt gagtgaaata tatgatgcgc
     8161 ctgtactttt attttacaag ggaaatcttg acctcctgaa attcccgaag gtagcggtcg
     8221 tgggcagtcg tgcttgtagc aaacagggag ctaaatcagt tgaaaaagtc attcaaggct
     8281 tggaaaatga actggttatt gtcagtggtc tggccaaggg cattgacaca gcagctcata
     8341 tggcagctct tcagaatggc ggaaaaacca ttgcagtgat tggaacagga ctggatgtgt
     8401 tttatcctaa agccaataaa cgcttgcaag actacatcgg caatgaccat ctggttctaa
     8461 gtgaatatgg acctggcgaa caacctctga aatttcattt tcctgcccgt aatcgcatca
     8521 ttgctggact ttgtcgtggt gtgattgtag cagaggctaa gatgcgttca ggtagtctca
     8581 ttacctgtga gcgagcaatg gaagaaggac gcgatgtttt tgctattcct ggtagcattt
     8641 tagatggact atcagacggt tgccatcatt tgattcaaga aggagcaaaa ttggtcacca
     8701 gtgggcaaga tgttcttgcg gaatttgaat tttaaaaatg acctaagcta gaattctaag
     8761 aaaaaatcaa ttttaagaga aaatgaaccc aacatttcca taataaaacg catattagca
     8821 agtttttaac acttgataat atgcgttttt tctaagtgga ttagtagagt agaggatttt
     8881 tctcatataa tactcttcga aaatctcttc aaactacgtc agcttccatc tgtaacctca
     8941 aaacagtatt ttgagctgac ttcgtcagtc ttatctacaa cctcaaagca gtgctttgag
     9001 caacctgtgg ctagcttcct agtttgcgct ttgattttca ttgagtataa gggaaagtat
     9061 agtgaattga aataagatgt gaacaactct atcaggaaag tcaaattaat ttatagaaat
     9121 attttagcag ccaaggtgca ctgttataga ttcaatacac tataatttag tgtaattgag
     9181 aaaggagaaa tgattgtgat tgatgttggc taggttatgt tcaatgattc ctaccgtctc
     9241 aaatcttgtc agtaaggaaa aataaattct tcaaaagtag agattacaag gcttgtttaa
     9301 gaaagaattc aaagaccttg acaaataaaa ataaaatggt tattataaaa aatggtctga
     9361 aatagatgat gatacttttc gaaaatcttt ttacgtcagc tcagctttgc cttgctgtgt
     9421 tttgagcaag ctacggttag cttccgagtt tgattttcat ttactagaaa tgaaactgat
     9481 gagagatatc agtagacatc tgagtcagga tattatggaa aatgataaaa agagctcgtg
     9541 agattggcat atcagactac taaagtattg agtttgttag gattttagcg actagttggc
     9601 tgggaaagga agatatttgt gacaaataat aaactgtatt cgttgataga atttagaaat
     9661 aaaatatatg aagaattaga actttccaga agtgatttag cgattttact atgtgccatg
     9721 cttatcgcct ctatcggatt aaatatggat tcgactcccg tgattattgg agccatgtta
     9781 atctctcctt tgatgacacc tattctggga atggggctct ctctagctat atttgatttt
     9841 aaattgttaa gaaaatcttt taaaatatta gctattcaaa ttcttgccag tctaatagct
     9901 tcaacacttt atttttatct ttctcccatt tcgtatgcta gttcggagat tgttgctaga
     9961 acctctccga ctatttggga tgttctcatt gcttttgtag gagggatagc aggtattatt
    10021 ggtgctagga aaaaagagac caataatatt gttcctggtg ttgctattgc aaccgccttg
    10081 atgcctcctc tttgtacagt aggttatgct attgcttctg ctaatctaaa atttatcata
    10141 ggctcctctt acctattcct catcaattgt agctttattg tcattgcgac ttatataggt
    10201 gttaggttga tgatggttaa gaaacattat tttaaagata atgaagaaga ctctaaaatg
    10261 cgtaggattt tgcttctagt tgctgttttg ctgatgattc cgagtttcat ctctgcaacg
    10321 actttagtga gagaaacgtt gaaaaaagag tcccttaaga aatttatatc agagcagttt
    10381 caggggtata atattttgaa aaaaacctat tctaaaaaga ctcataccct aaagctaacc
    10441 atttcaggaa attatttgac agaagaagaa ctcgatatga tttcaagtaa gagaggtgac
    10501 tatggtttaa gtgatgtttc tgttcaagtt tcacaattgt ctgattcaga acaacttagt
    10561 aaggaagagc tggtggagta tttcttccag tatatcaagg ataaggaagc aaaagaaaag
    10621 gagaaagcca ataaatttga tacagagtct gaggagcaat aatttcttga gaatagctgg
    10681 tttctctcgt gagtcttcta tgtatatcaa aggaagactg aggttttaag tataaaactt
    10741 ttcttctatt atagtagcta agtcttgaca gggttcaaaa aatggtttac actttataaa
    10801 gtttattact ttgaaaaggt gtgatactgt ggctacggca acaaaaaaga aaaaatcaac
    10861 agttaaaaaa aatctagtca tcgtggagtc gcctgctaag gccaagacga ttgaaaaata
    10921 tctaggcaga aactacaagg ttttagccag tgtcgggcat atccgtgatt tgaagaaatc
    10981 cagtatgtcc gtcgatattg aaaataatta tgaaccgcaa tatattaata tccgaggaaa
    11041 aggccctctt atcaatgact tgaaaaaaga agctaaaaaa gctaataaag tttttctcgc
    11101 gagtgacccg gaccgtgaag gagaagcgat ttcttggcat ttggcccata ttctcaactt
    11161 ggatgaaaat gatgccaacc gtgtggtctt caatgaaatc accaaggatg cagtcaaaaa
    11221 tgcttttaaa gaacctcgta agatcgatat ggacttggtc gatgcccaac aagctcgtcg
    11281 gatcttggat cgcttggtag ggtattcgat ttcgcctatt ttgtggaaga aggtcaagaa
    11341 gggcttgtca gcaggtcgcg ttcagtccat tgcccttaaa ctcatcattg accgtgaaaa
    11401 tgaaatcaat gccttccagc cagaagaata ctggacagtt gatgctgtct ttaaaaaggg
    11461 aaccaaacaa tttcatgctt ccttctatgg agtagatggt aaaaagatga aactgaccag
    11521 caatgacgaa gtcaaggaag tcttgtctcg tctgactagt aaagactttt cagtagatca
    11581 ggtggataag aaagagcgca agcgcaatgc tcctttaccc tataccactt catctatgca
    11641 gatggatgct gccaataaaa tcaatttccg tactcgaaaa accatgatgg ttgcccaaca
    11701 gctctatgaa ggaattaata tcggttctgg tgttcaaggt ttgattacct atatgcgtac
    11761 cgattcgact cgtatcagtc ctgtagcgca aaatgaggcg gcaagcttca ttacggatcg
    11821 ttttggtagt aagtattcta agcacggtag caaggtcaaa aacgcatcag gtgctcagga
    11881 tgcccatgag gctattcgtc cgtcaagtgt ctttaataca ccagaaagca tcgctaagta
    11941 tctggacaag gatcagctca agctatatac ccttatctgg aatcgttttg tggctagcca
    12001 gatgacagcg gccgtttttg ataccatggc tgttaaattg tctcaaaaag gggttcaatt
    12061 tgctgccaat ggtagtcagg ttaagtttga tggttatctt gccatttata atgattctga
    12121 caagaataag atgttaccgg acatggttgt tggagatgtg gtcaaacagg tcaatagcaa
    12181 accagagcaa catttcaccc aaccgcctgc ccgttattct gaagcaacac tgattaaaac
    12241 cttagaggaa aatggggttg gacgtccatc aacctacgcg ccaaccattg aaaccattca
    12301 gaaacgttat tatgttcgcc tggcagccaa acgttttgaa ccgacagagt tgggagaaat
    12361 tgtcaataag ctcatcgttg aatatttccc agatatcgta aacgtgacct tcacagctga
    12421 aatggaaggt aaactggatg atgtcgaagt tggaaaagag cagtggcgac gggtcattga
    12481 tgccttttac aaaccattct ctaaagaagt tgccaaggct gaagaagaaa tggaaaaaat
    12541 ccagattaag gatgaaccag ctggatttga ctgtgaagtg tgtggcagtc caatggtcat
    12601 taaacttggt cgttttggta aattctacgc ttgtagcaat ttcccagatt gccgtcatac
    12661 ccaagcaatc gtgaaagaga ttggtgttga gtgtccaagc tgtcatcagg gacaaattat
    12721 tgagcgaaaa accaagcgta atcgcctctt ctatggttgc aatcgctatc cagaatgtga
    12781 atttacctct tgggacaagc ctgttggtcg tgactgtcca aaatgtggca acttcctcat
    12841 ggagaaaaaa gtccgtggtg gtggcaagca ggttgtttgt agcaaaggcg attacgagga
    12901 agaaaagatt aaataagagg aggggcccga aagtaaattt caggctcctt ttggttagaa
    12961 cttgacaaaa tacatcattt tatgcgaaac tagaagaaga ttattttaac taggagaagt
    13021 tatgcgtctt atctatctga taattggttt tttatcactg gccttggcta ttgttggggt
    13081 tgttttaccc ttgttgccga caacgccttt ccttttgttg tctattgctt gtttctccag
    13141 aagttccaag cgtttcgaag actggcttta tcataccaag ctctatcaaa catatgtagc
    13201 tgattttcgc gagaccaagt ctattacgcg tgaacgaaag aaaaaaatca tcgtctctat
    13261 ctacgtcttg atgggaattt ctatttattt tgcacctctt ttaccagtca aaatcggttt
    13321 gggtgctttg accatcttta tcacttatta tctcttcaag gtcattccag acaaagaata
    13381 gttaaaacag tagttattta ccttgataaa attgaaagca tattcataac aatatgatat
    13441 aataaatttg aagtaatatt caaggagaat caaatgattt acgaattttg tgctgaaaat
    13501 gtgactttac ttgaaaaagc gatgcaggct ggagctcgtc ggattgaact ctgtgataat
    13561 ctagcagttg gtgggacaac acctagctat ggagtgacta aggcagcggt tgaactggca
    13621 gctaactacg atacaaccat catgaccatg attcggccac gtggtggtga ctttgtctat
    13681 aatgacctag aaattgctat catgctagaa gacatttgtt tgactgctca ggctggaagt
    13741 caaggggttg tatttggagc tttaactgct gataaaaagt tggataagcc taatctggaa
    13801 aagttaattg ctgcatcaaa aggaatggaa attgtctttc acatggcctt tgatgaacta
    13861 agtgatgaag atcaaccgga agctattgac tggctcagtc aagccggtgt cactcgtatc
    13921 ctaactcgtg ctggtgtgtc tggcgactcc ttagaaaaac gttttgttca ctatcacaga
    13981 attttggagt acgctaaagg taaaattgaa attctaccag gtggggggat tgaccttgac
    14041 aaccgtcaaa cctttattga ccaggtgggg gtaacacaat tgcatggtac taaggttgtt
    14101 ttttaaaaaa tagaaaggaa ctgctagcct tgggtagcag ttttcactta tgtttgaaat
    14161 ttttaaatcc tatcaattta atcaagaaaa ggctcatgat tatggtttta tagaaaatag
    14221 cgaagtctgg acatatagtt gccagatttt gcaaggtgac tttgtcatga ctgtgtccat
    14281 cactgctgat aatgtgaact ttcaagtctt tgaccaagag actggtgacc tctatcctca
    14341 cgttcatatg gaaagcatga ggggaagttt tgtcggaaat gtccgtgagg cttgtctgga
    14401 gattctttac cagattcgga aggcttgttt tgatgtgcaa gattttatct gtcatcagac
    14461 taagcgtatc atgactcaag ttcaggaaaa gtatggaaac cagttggagt atctgtggga
    14521 aaaatagcct gatacagctg tattgcgcca tgaaggcaat caaaagtggt atgccgtctt
    14581 gatgaaaatc tcttggaata agctggaaaa gggcagagaa ggacaagtgg aagcagtcaa
    14641 cctcaagcat gaccaagtag ctaatttgct ttcacaaaag gggatttatc cagccttcca
    14701 tatgagcaag cgctactgga ttagtgtgtc ccttgatgat actttatcag atgaagaagt
    14761 actggaattg atagaaaaaa gttggaactt aacctctaaa aaatgaaata ttttaataat
    14821 tttcatgaac tttcaattag ctaaatattc tttactgaag aaatttttag aaaatatagg
    14881 atttaccaca ctagaggaat atggtgccat cttcaaatac ctgattgaga atgtcaagac
    14941 ggatcgtcag atcatctgtt cgcctcactg tcatgatgac ctcggaatgg cagtggcaaa
    15001 tagccttgct gctgtcaaga atggtgcagg acgtgttgaa gggactatca atggtattag
    15061 ggagcgagct gaaaatgctg ctttggaaga aattgcagtg gctctcaata ttcgccaaga
    15121 ttactaccaa gtagaaacca gtattgtcct aaatgagacc atcaatacgt cagaaatggt
    15181 ttctcgcttc tctggtattc cagttcctaa aaacaaagcc gtcgttggtg gcaatacctt
    15241 ctcccacgaa tctggtattc accaagatgg agtccttaaa aatcctctca cttatgagat
    15301 catcacacct gaattggttg gtgttaagat tccgcttgga gaattatctg gtcgccatgc
    15361 ttttgttgag aaactgagag aattggccct agattttaca gaagaggata tcaaaccact
    15421 ctttgctaag ttcaaggcac tggccgataa gaagtaagaa atcacagatg cagatactcg
    15481 agctttggta gctggaacca tggttgaaaa tccagaaggc ttccactttg atgatttaca
    15541 acttcaaact catgcagata atgacattga agcgctcgtt agcctagcca atatggatgg
    15601 tgagaaagtc gaatttaatg cgacagggca aggttccgtt gaagcaatct ttaacgctat
    15661 cgataagttc tttaaccaat ctgttcgttt ggtgtcctac actatcaatg cggtaacaga
    15721 tggaatcgat gcccaggatc gggttttggt cactgttgaa aacagagata cagaaaccat
    15781 ctttaatgca gcagggcttg attttgatgt gttgaaggct tctgctattg cctatataaa
    15841 cgctaatacc tttgttcaaa aagagaatgc aggtgagatg ggacgcagtg tttcttacca
    15901 cgatatgcct agtgtgtaaa ggagaaggct atggcaaaga aaatagtagc tctagtagga
    15961 gacggaattg gcccagaaat catggaggct ggtttagaag ttctggaggc tctagctgaa
    16021 aaaacaggtt ttgactatga gattgacaga cgaccgttcg gaggtgcaga tattgatgca
    16081 gcaggacctc ccttacctga tgaaaccctt aaggcaagta gggaagcaga tgctatccta
    16141 ctagtagcta tcggtagtcc tcagtatgat ggagtagcgg ttcgccctga acaaggcctg
    16201 atggctctcc gtaaggaact caatctttac gctaatattc gtcctgtaaa aatctttgac
    16261 agtctcaagt atttgtcacc actcaaaccg gaacgaattt ctggtgtaga ctttgtcgtg
    16321 gtgcgtgaat tgacaggcga gatttacttt ggagatcata ttcttgaaga gcgcaaagcg
    16381 cgtgatatca acgactatag ctatgaggaa gtggagcgga ttattcgcaa agcctttgaa
    16441 attgcaagaa atcgcagaaa aatcgttact agtatcgata agcaaaatgt tctagcgacc
    16501 tcaaaactct ggcggaaagt agctgaggaa gtcgcacagg atttcccaga tgtaaccttg
    16561 gaacaccagc tggtagactc agctgctatg cttatgatta ccaatcctgc taagtttgat
    16621 gttattgtaa cggagaatct ttttggagat attttatctg atgaatcaag cgtcttatct
    16681 ggtacacttg gggttatgcc atcagccagt cattctgaaa atggaccaag tctctatgaa
    16741 cctattcacg gttcagcacc tgatattgca ggtcaaggaa ttgccaatcc tatttccatg
    16801 attttatcag ttgtcatgat gttgagagat agtttcggac gttatgagga tacagagcgt
    16861 atcaaacgtg ctgttgagac aagtctggcg gcaggaattt taacgagaga tataggaggt
    16921 caggcttcaa caaaggaaat gatggaagct attattgcaa ggttatgaag ttagacgaaa
    16981 aaattactct agtccttttg atttggaatg tcatcatttt cttgatttat ggcattgaca
    17041 aattcaaggc aagaagaaga gcttggcgca tcccagagaa aatcttactc attttagccc
    17101 ttacttgtgg tggttttggg gcctggttag ctggaatcac ttttcaccac aagactcgaa
    17161 aatggtactt taaaacagtt tggtttcttg ggatggtgac cacactagta gccttatatt
    17221 ttatttggag gtaatggatg gcagggtctt cgagggaata cgctgcttgg gctctagcgg
    17281 actatggttt taaggtcgtg attgcaggat ctttcggtga cattcattac aataatgaac
    17341 tcaataatgg catgttgcca atcgttcagc ctagagaggt tagagagaaa ctagcccagc
    17401 taaaaccaac cgaccaggta actgtggact tggaacaaca aaaaatcatc tcaccagttg
    17461 aagaattcac cttcgagata gatagcgagt ggaaacataa actcctaaat agtttggatg
    17521 atatcggtat taccttgcag tatgaagagt tgattgctgc ttatgaaaaa caacgaccag
    17581 cctactggca ggattagaaa aaatagaaaa ggaaatatag taaactgaaa taagatgtaa
    17641 acaaatgaat tggagcttaa catccatttc cagcaatttt ttagaaacta cagtggacta
    17701 ttctggattc aacacattat aaaattatga caaaacacat tcacaagaag gctacgacat
    17761 tttaaaaggt gagggcggat gtatcgtttg ccctactaaa gttggttaca ttatcatgac
    17821 cagtgacaag gcaggacttg agcgtaagtt cgcagccaaa gaacgtaagc gtaacaaacc
    17881 aggtgttgtt ctctgcggta gcatggatga acttcgcgct ttagcgcaac tcaacccaga
    17941 aattgaagca ttctactaaa aacattggga tgaagatatt cttcttggtt gtatccttcc
    18001 ttggaaacca gaagcctctg aaaaactcaa agcatacggg gatggccgtg aagaacttat
    18061 gactgatgta cgtggtacta gctgttttgt tatcaagttt ggaaaagcag gtgaacaatt
    18121 ggctgccaag ctttgggaag aaggtaaaat ggtctacgcc tcatctgctt caatgacaaa
    18181 acgattgaaa ctcgctatga gagcaaggtg taatggtgtc tatggtcgat aaggacggca
    18241 aactcatccc agaacaagga ggagcacgtt caacttcacc agcaccagtt gtgatccgta
    18301 aagggcttga cattgatata gtaaaatgaa gtaagagcag gacaaattaa tcaggacagt
    18361 caaatcgatt tctaacaatg ttttagaagt agaggtgtac tattctagtt tcaatccact
    18421 ataaaatcat gatgcacctg tcagatactt ttaactcatg ggactaccgt caggttgagt
    18481 attattagga tagagaagaa gtctagtgtt aaggacatta cagctcataa tactaggctt
    18541 ttttatagaa attcttttct ttttctatag gatatggtat tct
//
LOCUS       DAVKQL010000044        17074 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVKQL010000044 DAVKQL010000000
VERSION     DAVKQL010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17074
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.044"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            <1..419
                     /locus_tag="VW561_001483"
     CDS             <1..419
                     /locus_tag="VW561_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943726.1"
                     /translation="KDFDTKLTNEYKKISQFRSKVSQSPWIKYLEISSFILTIFPSIL
                     YGTVDNHVLTLIFLIKICVDTTIKFLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYL
                     LVIQKAGYLFSILLLFSNFSVPFIKEKEYELFKNSK"
     gene            1551..1790
                     /locus_tag="VW561_001484"
     CDS             1551..1790
                     /locus_tag="VW561_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943727.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     DLHKITRDYASQTKPAIQDGVFILEDFRNILCTMM"
     gene            2343..4352
                     /gene="pspA"
                     /locus_tag="VW561_001485"
     CDS             2343..4352
                     /gene="pspA"
                     /locus_tag="VW561_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein A"
                     /protein_id="HEU9943728.1"
                     /translation="MNKKKMILTSLASVAILGAGFVASSPTVVRAEDAPVANQSKAEK
                     DYDAAMKKSEAAKKHYEAAKKKAEDAQKKYDEDQKKTEEKAAKELKASKEIAEATSQV
                     QNGYVEYLKVQSATNINIKERQKKLAEIDEKINNAQQTLKKKQEELGKVRAEVIPEPA
                     ELAKTRKKAEEAKAEEKVAKKKYDEATQEVALAKKKVETEEAELDKKVAELQNKVTDL
                     EKEIADAEKTVADLEKEVAKLEKDVEGFKESDGEYAEFYLEAAEKDLATKKAKLAEAK
                     IKAATKKAELEPELEKAEAELENLLSTLDPEGKTQDELDKEAEEAELDKKADELQNKV
                     AELEEELSKLEDNLKDAEINNVEDYIKEGLEEAIATKQAELEKTQKELDAALNELGPD
                     GDEEETPAPAPQPEKPAPAPKPEQPAPAPKPEKSADQQAEEDYARRSEEEYNRLTQQQ
                     PPKAEKPAPAPKPEQPAPAPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNANG
                     SMATGWAKVNGSWYYLNANGAMATGWLQYNDSWYYLNASGAMATGWAKVNGSWYYLNA
                     NGAMATGWLQNNGSWYYLNANGAMATGWAKVNGSWYYLNANGSMATGWVKDGDTWYYL
                     EASGAMKASQWFKVSDKWYYVNGLGALAVNTTVDGYTVNENGEWV"
     gene            4753..5874
                     /gene="mnmA"
                     /locus_tag="VW561_001486"
     CDS             4753..5874
                     /gene="mnmA"
                     /locus_tag="VW561_001486"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEU9943729.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFSLGHLKKPEVRKLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            6015..6470
                     /locus_tag="VW561_001487"
     CDS             6015..6470
                     /locus_tag="VW561_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9943730.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYIIGGA
                     IQVNEKTEDAVVREVKEELGVKAQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDIDKLEGINLVPAFLKTALPDWEGQLRHIHLEE"
     gene            6480..8393
                     /gene="mnmG"
                     /locus_tag="VW561_001488"
     CDS             6480..8393
                     /gene="mnmG"
                     /locus_tag="VW561_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEU9943731.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDAVPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            8582..8671
                     /locus_tag="VW561_001489"
                     /pseudo
     CDS             8582..8671
                     /locus_tag="VW561_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(8773..10452)
                     /locus_tag="VW561_001490"
     CDS             complement(8773..10452)
                     /locus_tag="VW561_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU9943732.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(10454..10687)
                     /locus_tag="VW561_001491"
     CDS             complement(10454..10687)
                     /locus_tag="VW561_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEU9943733.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            complement(11505..11657)
                     /gene="cibB"
                     /locus_tag="VW561_001492"
     CDS             complement(11505..11657)
                     /gene="cibB"
                     /locus_tag="VW561_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEU9943734.1"
                     /translation="MMKDLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(11660..11845)
                     /gene="cibA"
                     /locus_tag="VW561_001493"
     CDS             complement(11660..11845)
                     /gene="cibA"
                     /locus_tag="VW561_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEU9943735.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            12023..12706
                     /gene="tsaB"
                     /locus_tag="VW561_001494"
     CDS             12023..12706
                     /gene="tsaB"
                     /locus_tag="VW561_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU9943736.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMESLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAYTLNIELVGMSSLLALVPY
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMAEAHLPFERVIELIKGASQVTFVGEVGPF
                     VEQIQKHLPRTDYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            12703..13140
                     /gene="rimI"
                     /locus_tag="VW561_001495"
     CDS             12703..13140
                     /gene="rimI"
                     /locus_tag="VW561_001495"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEU9943737.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RQSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            13130..14140
                     /gene="tsaD"
                     /locus_tag="VW561_001496"
     CDS             13130..14140
                     /gene="tsaD"
                     /locus_tag="VW561_001496"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU9943738.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLSLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVLDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEVTDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(14180..15486)
                     /locus_tag="VW561_001497"
                     /pseudo
     CDS             complement(14180..15486)
                     /locus_tag="VW561_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(15825..16113)
                     /locus_tag="VW561_001498"
                     /pseudo
     CDS             complement(15825..16113)
                     /locus_tag="VW561_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(16244..16513)
                     /locus_tag="VW561_001499"
                     /pseudo
     CDS             complement(16244..16513)
                     /locus_tag="VW561_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            16756..>17074
                     /locus_tag="VW561_001500"
     CDS             16756..>17074
                     /locus_tag="VW561_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU9943739.1"
                     /translation="MQYSCGKININIPDGYGDIKDIVFSAHIIVRYNNGHCGGIDPHI
                     IGLCKKQIRRMSLYPILIIVSRDSKVIDDYKNLDIAYVDCTQCSNNFETALHVKNILK
                     LLKI"
BASE COUNT         5219 a         3141 c         3825 g         4889 t
ORIGIN      
        1 ggaaagattt tgatactaaa ttgactaacg aatataagaa aattagtcag tttagaagca
       61 aggtgtctca atctccttgg ataaaatatt tagagatttc tagttttata ttaactatat
      121 ttccatctat tctttatggt acagttgata atcatgtact gactcttatc tttttgataa
      181 aaatttgtgt agatactacg ataaaatttt tattcatcag attatttgac acaagtactt
      241 taatgaagag gagaatattt tttcttttcg cattggatgt tatagcttac ttatttttag
      301 gatatctttt agtgattcaa aaagcaggat atctgttttc gattttactt cttttctcta
      361 atttttcagt tccatttatc aaagaaaagg aatacgaatt atttaaaaat agcaagtaat
      421 agaaacatag gttaaatctt agaacggctt aaaatcagat atgataaagg gaaatagtct
      481 tatctaggta actaagtgct acaaacacaa tcgaggatca cttgttagga ttacatttta
      541 atcatgctta ataagaatgg agaattccta tggggattgt attactctga agcttcgaat
      601 accttagttc tcgtgatttt gagctattct aggtttagaa tccttcagaa tgtaaaattt
      661 taagcagtca aagacttaga atagcctgaa aacatttatt ttataacaat tctaggctta
      721 gaattgctca tatatatgta tttgagctgc tcgatagtta ggacagctct attctgagaa
      781 atctgagcta tcaatgcttc agaatagtat ttcgccaatg gtttttattg aagaggtgat
      841 tgtgttatga tagtctaaaa taaaattgat ttaatagagt aaatggagaa tcgttacatg
      901 aaacattttt ttgcaggaat tggtgagata aaacttgttg gctacttatt gtatatatgt
      961 gtaggactag cccctctttt tcatatatat gtcataggtt cggaactgag ttttgtgaaa
     1021 atagtgttat ctattttggg agttatattt gtaagtatgt taactattgc tcgaatttat
     1081 cggacttttc tataaagaat agtttggact tgaactgtat ctaaaaacag aggttggtag
     1141 attatcagcc tctttcttgt cgttgaaaag agtgagatat attgactttt gaaaaaaatg
     1201 tcataattga gttgggataa acctaaaagt aatatagtag attgaatctg gaatagtccg
     1261 ttatgactgt taaaatattt ctagaaatta atttgacttt cctaatcgat ttgttcacat
     1321 cttgtttcaa tctactatac tttctcgtta aaccgttcgg taattcatgg tgacatggac
     1381 acatagtagt aagcacacta tgtggcttgg cagatctaaa aactgttccc ttgcgataag
     1441 cctaataagc acaacatagg gagttagaga agccgactct aatcaaccac tttgggcagt
     1501 agtggtaact gccccgtgct ttttattttg agaaaatatg gagtttgtcg ttgaaattac
     1561 ttgattgtat tttggattat caagaaagat tcaatggaaa aacatgtcaa gtatcaacga
     1621 attataagta tttagagatt ttcaaagtga atttttgctt gactgattta catcatttat
     1681 ttgacttaca caaaatcaca cgagattatg ctagtcaaac aaaacctgct attcaagatg
     1741 gtgtttttat tttagaagat tttagaaata tcctatgtac aatgatgtaa tcgaaaggat
     1801 atctttatgt gagtttatcg gcgatatctt ctatatctaa gctagcaagt tgttgcatcg
     1861 tagctaagga tttatctaaa aatactatga aattggacgt catatttttt gaggataaaa
     1921 ataaaagatc cgcagtttta ggtttacgaa gagacaaaag cggagtattt aaaccagtta
     1981 ctctacattt tacaagcgct aagaaatatg ctaaagttcg taaaacagat gtgaaagaaa
     2041 tgaaatggct ataaagattg tccgcaggct taagcttgcg ttttttgttg tttaacgaaa
     2101 ttaaaaaaca agctctctca tcggaaatgt ttttatagga atgaaggaag atgatgctat
     2161 gtttaaaaag agaatcgaaa agggcaaaag tagtatcttt atctattttt tggagtagaa
     2221 gcttatgata tagaaatttg taataaaaat gtaatataaa acatttgaca aatatttacg
     2281 gggggagtat acttaatata attatagtct aaaaatgact atcagaaaag aggtaaattt
     2341 agatgaataa gaaaaaaatg attttaacaa gtctagccag cgtcgctatc ttaggggctg
     2401 gttttgttgc gtcttcgcct actgttgtaa gagcagaaga cgctcctgta gctaaccagt
     2461 ctaaagctga gaaagactat gatgcagcaa tgaaaaaatc tgaagctgct aagaaacatt
     2521 atgaagcggc taaaaagaaa gcagaagacg ctcagaagaa atatgatgag gatcagaaga
     2581 aaactgagga aaaagctgct aaggaattaa aagcatcgaa ggaaatagca gaagccacgt
     2641 ctcaagtcca aaatgggtat gtagaatatc ttaaagtcca atctgctact aatataaata
     2701 taaaagaacg acaaaaaaaa ttagcagaaa ttgatgaaaa aataaataat gctcagcaaa
     2761 ctttaaagaa gaagcaggaa gagcttggta aagttagggc agaagtaatt cctgaaccag
     2821 ctgagttagc taagactcgg aaaaaagcag aagaggctaa agcagaagaa aaagtagcta
     2881 agaaaaaata tgatgaggca actcaagagg tagcactagc gaagaagaaa gtagaaacag
     2941 aagaagctga gttggataaa aaagttgctg aacttcaaaa taaagttact gatttagaaa
     3001 aagaaatagc tgatgctgaa aaaacggtag cagatttaga aaaagaagtt gctaaacttg
     3061 aaaaagatgt agaaggtttc aaagaatcag atggtgaata tgccgaattc taccttgaag
     3121 ctgctgaaaa agacctagct actaaaaaag ctaaattagc agaagctaaa attaaagctg
     3181 ctactaaaaa agctgaatta gaacctgaat tagaaaaagc agaagctgaa cttgaaaacc
     3241 tattatctac actagaccct gaaggtaaaa ctcaagatga attagataaa gaagcagaag
     3301 aagctgagtt ggataaaaaa gctgatgaac ttcaaaataa agttgctgaa ttagaagaag
     3361 aactttcaaa acttgaagat aatcttaaag atgctgaaat aaacaacgtt gaagactaca
     3421 ttaaagaagg tttagaagaa gctatcgcga ctaaacaagc tgagttagaa aaaactcaaa
     3481 aagagttaga tgcagctctt aatgagttag gccctgatgg agatgaagaa gagactccag
     3541 cgccggctcc tcaaccagaa aaaccagctc ccgcaccaaa accagagcaa ccagctcccg
     3601 caccaaaacc agagaagtca gcagatcaac aagctgaaga agactatgct cgtagatcag
     3661 aagaagaata taaccgcttg actcaacagc aaccgccaaa agcagaaaaa ccagctcccg
     3721 caccaaaacc agagcaacca gctcccgcac caaaaacagg ctggaaacaa gaaaacggta
     3781 tgtggtactt ctacaatact gatggttcaa tggcaacagg ttggctccaa aacaacggtt
     3841 catggtacta cctaaacgct aacggttcaa tggcaacagg ttgggctaaa gtcaacggtt
     3901 catggtacta cctaaacgct aatggtgcta tggcgacagg atggctccaa tacaacgatt
     3961 catggtatta cctaaacgct agcggcgcta tggcaacagg ttgggctaaa gtcaacggtt
     4021 catggtacta cctaaacgct aatggtgcta tggcgacagg atggctccaa aacaacggtt
     4081 catggtacta cctaaacgct aacggtgcta tggcaacagg ttgggctaaa gtcaacggtt
     4141 catggtacta tctcaacgct aacggttcaa tggcaacagg ttgggtgaaa gatggagata
     4201 cctggtacta tcttgaagca tcaggtgcta tgaaagcaag ccaatggttc aaagtatcag
     4261 ataaatggta ctatgtcaat ggcttaggtg cccttgcagt caacacaact gtagacggat
     4321 atactgtcaa tgaaaacggt gaatgggttt aagccgatta aattaaatca tgttaagaac
     4381 atttgacatt ttaattttga aacaaagata aggttcgatt gaatagattt atgttcgtat
     4441 tctttaggta cctgtcttat gatttcagga aatgtcatta aaaaaacgac tccttttctc
     4501 taacttggaa aatagattag agaaaatggg ttgttttatt tattatagtt atttgaatga
     4561 agataagaag aaggtatatt cacatcattc acataatctg tatattgact ataagtttta
     4621 aaaaacaatt tttaagctct tccttgtctt ctccaaccaa gcgtgttata atgaatactg
     4681 ctcaagcgac cttcaatcgt gaagcacaca cgaccttcaa tcgtgaataa acgaatagat
     4741 gggagactta ccatgagtga taactctaaa acacgtgttg tcgtggggat gagtggtggt
     4801 gttgattcgt cggtgacggc tcttttgctc aaggagcagg gctacgatgt gatcggtatc
     4861 ttcatgaaga actgggatga cacagatgaa aacggcgtct gtacggcgac cgaagattac
     4921 aaggatgtgg ttgcggtggc agatcagatt ggcattccct actactctgt caattttgaa
     4981 aaagagtact gggaccgcgt ttttgagtat ttcctagcgg aataccgtgc agggcgcacg
     5041 ccaaatccgg acgttatgtg caacaaggaa atcaagttca aggccttttt ggactatgcc
     5101 atgaccttgg gggcagacta tgtagcgact gggcactatg ctcgagtggc gcgtgatgag
     5161 gatggcactg ttcacatgct tcgtggcgtg gacaatggca aggatcagac ctatttcctc
     5221 agccaacttt cgcaagaaca acttcaaaaa accatgttct cactaggaca tttgaaaaag
     5281 cctgaagttc gaaaactagc agaagaagca ggtctttcga ctgctaagaa gaaagactcg
     5341 acagggattt gctttatcgg agaaaagaac tttaaaaact ttctcagcaa ctacctgcca
     5401 gctcagcctg gtcgtatgat gactgtggat ggtcgtgata tgggcgagca tgctggtctt
     5461 atgtactata caatcggtca gcgtggcgga ctcggtatcg gtgggcaaca cggtggtgac
     5521 aatgcccctt ggttcgttgt cggaaaagat ctaagcaaga atattctcta tgtaggccaa
     5581 ggtttctacc atgattcgct catgtcaacc agcctagagg ctagccaagt ccactttact
     5641 cgtgaaatgc cagaagagtt tacgctagaa tgtacggcta aattccgtta ccgtcagcct
     5701 gactctaagg tgaccgtcca tgtcaaagga gacaaggcag aagttatctt tgcggaacca
     5761 caacgcgcga ttacaccagg acaggcagtt gtcttttacg atggcgaaga gtgtctaggt
     5821 ggcggtttga ttgacaatgc ttaccgcgat ggacaagttt gtcagtacat ttagattgac
     5881 aaattttctc aatttgctac aataataaca gcaatagaaa tgatggtcaa agctcatgga
     5941 tgttgcaggc ttttttgtcc tgcacttctt tgtagttttg actgtttttg tgtcgtttaa
     6001 gggaaaggac aagaatgact caacaagact ttcggacaaa agtagacaat acggtttttg
     6061 gtgttcgggc gacagccttg attgtccaaa atcacaagct tctagtcacc aaagacaagg
     6121 gcaagtatta cattatcggc ggtgcgattc aagttaacga aaaaacggaa gacgcggtag
     6181 tccgtgaagt gaaggaagaa ctgggtgtta aagctcaagc tgggcagcta gcttttgtgg
     6241 ttgaaaatcg ttttgaagtg gacggcgttt cctatcacaa tatcgaattt cattatctgg
     6301 tggatttgct tgaagatgcc ccgttgacca tgcaggaaga tgagaaaagg cagccctgtg
     6361 aatggattga catagacaag cttgagggta tcaatctagt tccagccttt ttaaaaacgg
     6421 ccctaccaga ttgggaaggc caactaagac acattcatct tgaggaatag gagagaaaca
     6481 tgacttatca ttttactgaa gaatacgata ttattgtaat tggtgcggga cacgctgggg
     6541 ttgaggcttc cttggccgct agccgtatgg gctgtaaggt cctgcttgcg accatcaata
     6601 ttgaaatgct ggctttcatg ccttgtaatc cctctatcgg tggttctgct aaggggattg
     6661 tcgtacgtga agtcgatgcc ctcggtggcg agatggccaa gactattgac aagacttaca
     6721 tccagatgaa gatgctcaac acaggaaagg gtcctgccgt tcgtgccctt cgtgcgcagg
     6781 ctgataagga actttactct aaggaaatgc gcaagacagt tgaaaatcaa gaaaatctga
     6841 cccttcgtca aaccatgatt gatgagattt tggtggaaga tggcaaagtt gtcggtgttc
     6901 gtacagcgac tcatcaagag tatgctgcta aggctgttat tgtgacgaca gggactgctc
     6961 tccgtgggga aattatcatc ggagacctca agtactcatc aggtcctaac cacagcttgg
     7021 cttctattaa cctagctgac aatctcaagg aactgggtct cgaaatcggt cgtttcaaga
     7081 caggaacccc tccacgtgtc aaggcctctt ctatcaacta cgacgtgaca gaaatccaac
     7141 caggagacgc agtacctaat catttctcat acacttcacg tgatgaggat tatgtcaaag
     7201 atcaagtgcc atgctggttg acctatacca atggtaccag tcatgagatt attcaaaaca
     7261 acctccaccg tgcgcctatg tttacaggtg tggtcaaggg agtggggcct cgttactgtc
     7321 cgtcgattga agacaagatt gtgcgctttg cggacaagga acgtcaccaa ctcttccttg
     7381 agccagaagg gcgcaatact gaggaagtct atgttcaagg actttcaacc agtctgcctg
     7441 aggatgtcca gcgtgacttg gttcattcca tcaaaggttt ggaaaatgca gagatgatgc
     7501 ggacaggtta tgctattgag tatgatatgg tcttgcctca tcagttgcgt gcgactttgg
     7561 aaaccaagaa aatctcaggt ctcttcactg ctggtcagac aaatggaacg tcaggttacg
     7621 aagaggcagc aggccaaggg attatcgcag gtatcaatgc ggctctaaaa atccaaggca
     7681 agcctgagtt gattttgaag cgcagtgacg gctatatcgg ggtcatgatc gacgacttgg
     7741 tgaccaaggg aaccattgaa ccttaccgtc tcttgaccag tcgtgctgaa taccgtctca
     7801 ttcttcgcca tgacaatgcc gatatgcgtt tgacagagat gggccgtgag attggccttg
     7861 tggatgatga acgctgggct cgttttgaaa tcaagaaaaa tcaatttgat aatgagatga
     7921 aacgcctaga cagtatcaaa ctcaagccag tcaaggaaac caatgctaag gttgaggaaa
     7981 tgggcttcaa gccgttgaca gatgcggtga cagccaagga attccttcgc cgtccagaag
     8041 tttcttacca agatgtggtg gccttcatcg gaccagctgc agaagacttg gatgacaaga
     8101 ttatcgaatt gattgaaaca gaaatcaagt acgaaggcta tatttccaaa gccatggatc
     8161 aggttgccaa gatgaaacgt atggaagaaa aacgcattcc agccaatatc gactgggatg
     8221 acatcgattc tattgcgacg gaagctcgtc agaagttcaa actcatcaat ccagaaacca
     8281 tcggccaagc cagccgtatt tcgggagtaa acccagcaga tatttctatt ttgatggtgt
     8341 atctggaagg taaaaatcgt agtatttcta aaactcttca aaaatcaaaa tgatacgtcg
     8401 tcggcttctt acgaatgagt tcaaagcttg gctttgattc atctccaacc tcccatagtt
     8461 ccccgaacta tgggagctaa ctcaagttat gcattttcct agccttctgg tctgattttc
     8521 attgagtttt aatctcaaaa gaaagcatag agaaaaacag ctccgaagac ggggctgttt
     8581 tgttgatttg tactcaatga aaatcaaaga gcaaactagg aagctagccg taggttgctc
     8641 aaagcactgc tttgaggttg tagatagaac tgacgaagtc agctcaaaac accgttttga
     8701 ggttgtggat agaactgacg aagtcagtaa ccatatatac ggcaaggcga agctgacgtg
     8761 gtttgaagag tattattctt catctggtgt gaggatcatc gggatgatga tcggttcacg
     8821 ttcggtattt tcatagagga aggggcgaat agcgttgaca atggcaccat tgacagattg
     8881 cacgctagca tccttatttt tcagtgcgat acgaatggca ttgaagagga tacgctggct
     8941 ttggcgaatc aagtcgccag actctctcat gtagacaaag cctcggctga ggatgtctgg
     9001 accagataga atcatctgcg atttgaagtc aacagttgcg actgccagaa cgacaccgtc
     9061 ttcagataga tcgcgacgat ctttgaggac agctgcgcca atttcaccga tacgatttcc
     9121 atcgacatag atatcttggg cgttgaaatg acctgcgata cgagctgagt cagcagtaag
     9181 ggcaagcaca tcgccattgc tcatgataaa gatattgtcc ttctcaacac cagtatccac
     9241 tgctagtcca gcgtggactt tttgcatgcg gtattcaccg tggacaggca tgaagtattt
     9301 tggcttaatc aagcagagca tgagtttttg ctcttgctgg ccaccgtgtc cagatgtatg
     9361 gatattgttc actttaccgt ggataacttc gacaccagct tcagaaatga tgttaatcag
     9421 cttgttgacg ctagtagtgt ttccagggat gggactagaa gagaagataa cggtatcacc
     9481 tggttgtaat tgtacttgac ggtgggttcc gttggcgata cgagagaggg ctgccatagg
     9541 ctcaccctga ctacctgtac agaggataag aacttctcct gcaggataat ctttgatttc
     9601 atttggctcg ataaaggttc ccttaggagc tttgatgtag ccaagatcga ttccgttgac
     9661 aatggccttt tccatagaac gaccaaagac cgcaatcttg cgtccagtct taacagcagc
     9721 ttctgttgcc tgctggagac ggaagatatt tgaggcaaag gatgcaaaga tgatacgtcc
     9781 ttcaatacct tggataatct tcataatgga ctgaccaacg actttttcag agttggtaaa
     9841 ggttggtact tccgcatttg tcgagtcaga caggagacag agcacgcctt cttcaccaag
     9901 cgcagccata cgatgcaagt ccgcaggttc tccaactgga gtaaagtcga acttaaagtc
     9961 acccgtacag acgattttcc cttgaggagt atgaatgaca atccccaaag gctctggaat
    10021 agagtgagtc gttctaaaga aagttgcctt gagattttta aaggtcaact cggtgttgtg
    10081 gttgatttcg taaagtttgg cgttgcgcaa gaggccgtgt tcttcgagtt tcccacggat
    10141 caaagccaag gcaagcggtc cagcataaat agggacattt gcttgcttga gtaggaacgg
    10201 aatcccacca atgtggtcct cgtgtccgtg tgtgattaaa acagccttga cgcggtcgat
    10261 attgtctacg atgtaagagt agtcaggaat gacatagtcg ataccaagca agtcatcttc
    10321 tgggaattta atcccagcat cgacgatgat aatctcgtct tggtattcaa ttccgtaagt
    10381 gtttttcccg atttctccta gaccaccgat ggcaaaaacg ccgacttctt caggtttaag
    10441 agtgtaggcc atattagaac tccgtaattt cgaaggcgcc agtttctttt tcgtaatcga
    10501 gcaatttgtc agacaagagt tcgatatatt cgatattgta ctctgggcga ttttcttcga
    10561 caagttggcg agcagtgata cggccctcaa gttctgagct ggcatcgatg tctaggtaaa
    10621 gcgcgcgtgt tgtttcacgg cgtgggctac gttcttttgt ttcttgataa aaaactttgt
    10681 aaatcatata gttcctttct atttacaggt cagcttattc caaactttta gcagatattt
    10741 gcttgatttc attccatttt gtgtctagat tgaccttgat tcgttacaat tatttcaatt
    10801 ataccacaaa atgaaaaatt agtaaaagac ggaaacgaga aagttagggg acgatttctt
    10861 tggtaagcgt ttgcaaaaac aaggaaaata tattatgttg ataacggaag tgaaatttat
    10921 tgcttgtcat aattccatat agattttaag tcaaggaggt tgccctatgt ataacttatt
    10981 tgcttttgct atcggagttc gtttgttagc ttttatcgtt tttgttgctt gcgtttatgg
    11041 aggaaatctt ctatttgcca aactggaacg atgacaaacc aagttcttgt ggaaaattac
    11101 ctttgtgact ctctactcgc tttttctcct cctcgtgacc tatgtcgttt ggtttgtatt
    11161 ttactttgga ttaaatgctt agatgccctg cttatgctgg ggttttttgt ttggggatgt
    11221 agagatagta gatttacgac acgaaaattc caccccatct atcaagatga ggtggtcttt
    11281 gcatttttct aacatgacat gacaatggaa ttttcaaagc ttattatttt tatgggagtc
    11341 attgttctgc tcaaaaatag agttgaagca taaaaataaa acaatacaaa cagataaatc
    11401 gaatggtaaa gggatagtag taatgttcaa atcattgaga atgctgagca aactgtaaga
    11461 gatgaagaca ataaaattta tccttctaaa aatgtgtttc ttatctagca tgacttacca
    11521 aactttttac gaaggtcttt tccaatggta taacctgcca taaataaagc gataccaaca
    11581 cctacaccaa agccaccctt gatttcttgc aattccttat tagaaatttc acgatagttg
    11641 ttcaaatctt tcatcatagt taatctcctc ctaaaaaata accagctgcc aaaccagaac
    11701 ctataagtgc tgttccacct gcaatagcct tccaagatac tgctactcca aagataacca
    11761 agggagcgag accgccatca atatctgata attcattttc agataaggat aaaaattgat
    11821 tgtcaagaat gtcaaaattt gtcattgaac tttcctcctt ttttcttgtt cacttttata
    11881 ttcggaaaag aaaaggaaaa gtgagcattt tttttaatta tttttagtta agtgagggca
    11941 ctcttgtctg ggggcaaaga aaattcccat gtgccagctt ttgtagtata atagtaagca
    12001 gacaaaaaga taggaatgtg ttatgaaagt attagctttt gatacgtcca gcaaggctct
    12061 ttctctggct attttagagg acaagcaggt tcttgctgag acgacgatta atatcaagaa
    12121 aaatcacagt attaccctca tgccagccat tgattttttg atggagagtt tggactggac
    12181 gcccaaggat ttggaccgaa tcgtggtggc ggaagggcca ggtagctaca caggcttgcg
    12241 aatcgcggta gccactgcca aaactctggc ttatactctg aatatcgagt tggttggtat
    12301 gtcgagtctc ttggctctgg tgccctacca acaagaaggt ttgtttgtcc ccttgatgga
    12361 tgcgcgtcgc aataatgttt atgcaggatt ttatgaaaat gccaaacctg tcatggcaga
    12421 agcacatctg ccttttgagc gagtcattga gctaatcaag ggcgctagtc aggtaacctt
    12481 tgtcggagaa gttggcccct ttgttgagca gattcaaaaa cacttgccaa ggactgatta
    12541 caaagaaaca ttgcccaatg cagctaatct agctcttttg gcctgggaca aggaagcaga
    12601 ctccttgcat gattttgtgc caaattacct caaacgtgtt gaggcagagg aaaactggct
    12661 caagaaccat accgagtctg gtgagtccta cattaaacgc ctatgataga aatcaagcga
    12721 attcaacaac agcctgacct agctcaagcc atctacgctg ttatggcagc tgtttaccta
    12781 gtcagtcctt ggactctgga gcaaatccaa gcagatctgt cccaagacca gacttggtat
    12841 gcattggctt atgatggggc agaagtgatt ggatttctag ctgtgcagga gaatcttttt
    12901 gaagcagaag tcctgcaaat cgctgtcaaa ggagcttatc agggtcaggg aattgcgtca
    12961 gccttgtttg ctcaattgcc gacagacaag gaaattttcc tcgaagtcag acagtcaaat
    13021 caacgagcgc aagcatttta caagaaagaa aagatggcag ttatcgctga gcgaaaggcc
    13081 tactaccatg acccagtcga ggacgccatt atcatgaaga gagaaataga tgaaggatag
    13141 atatatttta gcatttgaga catcctgtga tgagaccagt gtcgccgtct tgaaaaacga
    13201 cgatgagctc ttgtccaatg tcattgctag tcaaattgag agtcacaaac gttttggtgg
    13261 cgtagtgccc gaagtagcca gtcgtcacca tgtcgaggtc attacagcct gtatcgagga
    13321 ggcattggca gaagcaggga ttaccgaaga ggacgtgaca gctgttgcgg ttacctatgg
    13381 accgggctta gtcggagcct tgctagttgg tttgtcagct gccaaggcct ttgcttgggc
    13441 tcacggactt tcactgattc ctgttaatca catggctggg cacctcatgg cagctcagag
    13501 tgtggagcct ttggagtttc ccttgctagc ccttttagtc agtggtgggc acacagagtt
    13561 ggtctatgtt tctgaggctg gcgattacaa gattgttggg gagacacgag acgatgcagt
    13621 tggggaggct tatgacaagg tcggtcgtgt catgggcttg acctatcctg caggtcgtga
    13681 gattgacgag ctggctcatc aggggcagga tatttatgat ttccctcgtg ctatgattaa
    13741 ggaagataat ctggagtttt cattctctgg tttgaagtca gcctttatca atcttcatca
    13801 caatgccgag caaaagggag aaagcctgtc tacagaagat ttgtgtgctt ccttccaagc
    13861 agctgtcttg gatattctca tggctaaaac caagaaggct ttggagaaat atcctgttaa
    13921 aaccctggtt gtggcaggtg gtgtggcagc caataaaggt ctcagagaac gcctagcagc
    13981 cgaggttaca gatgtcaagg tcatcatccc ccctctgcga ctctgcggag acaatgcagg
    14041 tatgattgcc tatgccagcg tcagcgagtg gaacaaagaa aacttcgcag gctgggacct
    14101 caatgccaaa ccaagtcttg cctttgatac catggaataa agagtggctc tttgtcaagt
    14161 gtagtgggta ggcgaaaagc tacaatctgg agagtacgaa attcgtactc tcttttttga
    14221 tgttcaaagt catcaaaatc cgttttttaa aattgttaaa gttccggaaa ccaaaggctt
    14281 tgcgcttgat aaccttaatc aaattattgg tcgcttcaag tttagcgttg gaataaggca
    14341 gcttaagtgc gttgataatc ttatctctat cccttaaaaa agtccaaaag accgtttgaa
    14401 aaagcggatg aaccgttggc agttcttgct caatcaatcc aaagaaatgg tcggcattct
    14461 tctcttgaaa atgaaagagc aggagttgat agagttcgta gtgaacctgt aatccctcag
    14521 agtaagaaag cagcttatct cgtacttccc gatgggttaa gtgcattcga aacgtgggct
    14581 gatagaatcg tttctgacag agcttgaagc tatcctggtg tagtaaatca atccagtgga
    14641 ctgatttagc ccgagcctag aaacgaaaaa gcgagggttc caaaagtgct tgagcgcccg
    14701 atactccaag gattttcggt caaattggtt catgatttgg attcgtactc ggttcatagc
    14761 tcggctcaga tgttggacaa tgtggaagcg gtcaagaaca atcttcgcct ttggaaagag
    14821 ttgcttagcg agccgataat aagggctgta catgtccatg gtgatgacct cgaccagctc
    14881 ccgaacctct ctctgatagc gttggaaatg gtttcgaatc accgcatgag ttcgcccgtc
    14941 taaaattgcg aggatggatt tggactcaaa atcttgggca atgaagctca ttttgctctt
    15001 tttgaagcta tactcatccc aactcataac ttttggcaac ttggtccaat cggtttcaaa
    15061 cttaaattcc ctcagttttc ggatgacggt ggaagttgag accgccaatc tgtgggcaat
    15121 ctcagtcata gactgctttt caaggaggag ctgagcgatt ttttgtctca ccatgttgga
    15181 aatctggcaa tttttcttga caatggatgt ttgagaaacg accaccttaa ggcaattctt
    15241 gcactgaaag cggcgctttt tgagatgcag taccgtgggt aaaccctgac agtcgagaag
    15301 cggaatttta gaggctttct ggaagtcgta tttgatcatc ttcccttgac aatgaggaca
    15361 aggaggagcg ggggaatcca actttgcctg aacgactaga tgggtttggt atttcagaac
    15421 agacaagatt ttgatatttt tgtcttccaa tccgagcaaa tctgtggtat tcttaagttg
    15481 ttccatatga cccctttcta atgagttggt tagacgcttt tcattatagg tcatatgggg
    15541 ctttttttct acaagaaacg accctataat tcctggggtg ggattaccca ctacagaaat
    15601 tatagagcca aaacattcca aagtcttgtc tgactctgga atgttttctg ttcacagttg
    15661 ttttgtaact gcctaataag taaccgcgct ataacgaggt atctaccgag attgtcaatt
    15721 ctccagtata ctgttataaa aaacaaaact ggaggatttt tttatgtccc cccatcgtcc
    15781 catcgactgt tccgtagctg cccaataacg gagtatagtc gtctgaaaca agaataggac
    15841 aaaagtgcat caagaaaagc atcgtaattt gacaatatta ttctgagatg tttttgatgt
    15901 aagcccatat tttctcaatg ggattatact cgggtgaata ggtaggaagt ggtaacagtc
    15961 tatgccctgc tccttacaca tgttcattct gtgaaacctt gcattgtcca taatgataag
    16021 ggatggtgta tctaaagtag gtagtaagaa tttttggaag caagcttcga aaaagtcact
    16081 cgtcatcgta tctttatagg tcatcggagc catagtatat tgaaacaaaa aagggtatta
    16141 gtacttctat aacagtatcg caatctctgg tgtgcgttgt ctcgatctta attcaaataa
    16201 ttatatcttc ttccagagag actaactgcc tgctcaagga tcaatcatat tcgagataaa
    16261 aataggtctc aacccctgtc tcataaatat aaataggagt caggtagctt aagtgattca
    16321 attctttaag gaaccgattt actttttctg ggtcttgttc gcagtaggta cggctctttt
    16381 ttagactata tcccatagcc tttagagcgt aatgaatagt tgttggagga cagttgaatt
    16441 cagcagctat ttcagtcaaa taagcatcgg gatgagtttc aatatagatc tttaatttat
    16501 ctctatcaac tttttcaatt aatagtggaa gttttcttga agttagtgta ttgaaacgtt
    16561 tttatacagt gctaaaattt gaagtgtagt gcagatgtat ttgtaatagt attatagcaa
    16621 ttctttggag aaggtcgaga caagagaaaa taggtatctt tgagatataa gcatgattta
    16681 ctataaaatt attgagttat tcataaaaat ttctctatca aatatcataa tgcgtcgttt
    16741 aaggagatgg ttacaatgca atatagttgt ggaaaaataa atattaatat ccctgatgga
    16801 tatggagaca ttaaggatat agtgttttcg gctcatatta tagttaggta taataatgga
    16861 cattgtggag gtatagatcc gcatataatt ggactttgta aaaagcagat cagaaggatg
    16921 tctctatatc ctattttgat aatcgtatct agagattcaa aggttattga tgattataaa
    16981 aatttagata ttgcctatgt tgattgtact caatgttcaa ataattttga aactgcctta
    17041 cacgttaaaa atattttaaa attattaaaa atcc
//
LOCUS       DAVKQL010000045        16757 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVKQL010000045 DAVKQL010000000
VERSION     DAVKQL010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16757
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.045"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(<1..285)
                     /locus_tag="VW561_001501"
     CDS             complement(<1..285)
                     /locus_tag="VW561_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEU9943740.1"
                     /translation="MKQTRNFDEWLSTMTDTVADWTYYTDFPKVYKNVSSIKVALNIM
                     NSLIGSKNIQEDFLDLYQNYPEILKVVPLLIAKRLRDTIIVKDPIKDFYFD"
     gene            complement(272..1042)
                     /locus_tag="VW561_001502"
     CDS             complement(272..1042)
                     /locus_tag="VW561_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEU9943741.1"
                     /translation="MTKPYYNKNKMILVHSDTFKFLSKMKPESMDMIFADPPYFLSNG
                     GISNSGGQVVSVDKGDWDKISSFEEKHEFNRKWIRLAKEVLKPNGTVWISGSLHNIYS
                     VGMALEQEGFKILNNITWQKTNPAPNLSCRYFTHSTETILWARKNDKKARHYYNYDLM
                     KELNDGKQMKDVWTGSLTKKVEKWAGKHPTQKPEYLLERIILASTKEGDYILDPFVGS
                     GTTGVVAKRLGRRFIGIDAEKEYLKIARKRLEAENETN"
     gene            complement(1068..1922)
                     /locus_tag="VW561_001503"
     CDS             complement(1068..1922)
                     /locus_tag="VW561_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEU9943742.1"
                     /translation="MKIKEIKKVTLQPFTKWTGGKRQLLPVIRELMPKTYNRYFEPFV
                     GGGALFFDLAPKDAVINDFNAELINCYQQIKDNPQELIEILKVHQEYNSKEYYLDLRS
                     ADRDERIDMMSEVQRAARILYMLRVNFNGLYRVNSKNQFNVPYGRYKNPKIVDEELIS
                     AISVYINNNQLEIKVGDFEKAIVDVRTGDFVYFDPPYIPLSETSAFTSYTHEGFSFAD
                     QVRLRDAFKRLSDTGAYVMLSNSSSALVEELYKDFNIHYVEATRTNGAKSSSRGKISE
                     IIVTNYEK"
     gene            complement(2079..2483)
                     /locus_tag="VW561_001504"
     CDS             complement(2079..2483)
                     /locus_tag="VW561_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU9943743.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERQVRI"
     gene            complement(2553..4034)
                     /locus_tag="VW561_001505"
     CDS             complement(2553..4034)
                     /locus_tag="VW561_001505"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEU9943744.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSRKNDYIKLKAIAKNIAYRIPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQTAFMRFVEHVGISLD"
     gene            complement(4053..5231)
                     /locus_tag="VW561_001506"
     CDS             complement(4053..5231)
                     /locus_tag="VW561_001506"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEU9943745.1"
                     /translation="MTQHLTAETLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            5395..6402
                     /gene="galR"
                     /locus_tag="VW561_001507"
     CDS             5395..6402
                     /gene="galR"
                     /locus_tag="VW561_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEU9943746.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFKNYSQARGIYHDELVFQGR
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALRALQEAGINLPDRVSLISYND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(6449..7486)
                     /locus_tag="VW561_001508"
     CDS             complement(6449..7486)
                     /locus_tag="VW561_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU9943747.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEVAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(7487..7840)
                     /locus_tag="VW561_001509"
     CDS             complement(7487..7840)
                     /locus_tag="VW561_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU9943748.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSACVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(8084..8974)
                     /locus_tag="VW561_001510"
     CDS             complement(8084..8974)
                     /locus_tag="VW561_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEU9943749.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGMSAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEYMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            9107..9643
                     /locus_tag="VW561_001511"
     CDS             9107..9643
                     /locus_tag="VW561_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU9943750.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFQKNQDHIASLLFS
                     KNDYFLRQLQRELEHHVYPMLAADLKQTHPSLPTSYLQHFVVTNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            complement(9810..10616)
                     /locus_tag="VW561_001512"
     CDS             complement(9810..10616)
                     /locus_tag="VW561_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEU9943751.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(10961..12481)
                     /locus_tag="VW561_001513"
     CDS             complement(10961..12481)
                     /locus_tag="VW561_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEU9943752.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLVQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            complement(12474..13202)
                     /locus_tag="VW561_001514"
     CDS             complement(12474..13202)
                     /locus_tag="VW561_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9943753.1"
                     /translation="MIEYKNVALCYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKVPATDFVADLFGGSVHD"
     gene            complement(13216..13980)
                     /locus_tag="VW561_001515"
     CDS             complement(13216..13980)
                     /locus_tag="VW561_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU9943754.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHDIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLKNGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(13984..14427)
                     /locus_tag="VW561_001516"
     CDS             complement(13984..14427)
                     /locus_tag="VW561_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9943755.1"
                     /translation="MKDGHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKSQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            complement(15109..15405)
                     /locus_tag="VW561_001517"
     CDS             complement(15109..15405)
                     /locus_tag="VW561_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU9943756.1"
                     /translation="MVEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLASFIKREEAKYRGTATSALMYEELFKML"
     gene            complement(15427..16491)
                     /gene="pepA"
                     /locus_tag="VW561_001518"
     CDS             complement(15427..16491)
                     /gene="pepA"
                     /locus_tag="VW561_001518"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEU9943757.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKLDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
BASE COUNT         4987 a         3937 c         2874 g         4959 t
ORIGIN      
        1 atcaaaatag aaatctttta tcggatcctt aacaataatc gtatctctca aacgtttagc
       61 aataagtaga ggcacaactt tcaaaatttc tggataattt tgatataagt ctaaaaaatc
      121 ctcttgtata tttttactac caataagact attcataata tttagagcaa cttttatact
      181 agatacattt ttataaacct taggaaaatc tgtataataa gtccagtcag ctactgtgtc
      241 ggtcatcgtt gacaaccact catcaaaatt tctagtttgt ttcattttct gcctccaacc
      301 tcttccttgc aatttttaaa tattcttttt cagcatcgat acctataaat cttctaccta
      361 accgcttcgc aacaacaccc gtagtgccac taccaacaaa tgggtctaga atatagtcac
      421 cctctttagt agaggctaaa ataatgcgtt ctaacaaata ctctggtttt tgagttggat
      481 gtttcccagc ccatttttca actttctttg ttaaagaacc ggtccagaca tctttcattt
      541 gttttccatc attcaattct ttcattaaat cataattgta gtaatgacga gcttttttat
      601 cattttttct ggcccataaa atggtttcag tagaatgggt aaaataacga caagataaat
      661 tgggggcagg gtttgttttc tgccaagtaa tattattcag aattttaaaa ccttcttgtt
      721 ctaatgccat tccaactgag tatatgttgt gcaaactacc tgaaatccat accgtcccat
      781 taggcttcag aacttctttt gctaggcgaa tccatttacg attaaactca tgtttttctt
      841 cgaatgaaga aattttatcc caatctcctt tatcaacaga aacaacttgt cccccagaat
      901 tagatattcc accattactt aaaaaataag gtggatcagc aaaaatcata tccatacttt
      961 ctggtttcat ttttgataag aacttgaacg tatctgaatg aacaagaatc atcttatttt
     1021 tattgtagta tggttttgtc ataagaacac ctccatactt atattcgtta tttttcataa
     1081 tttgtgacta taatttcaga aatttttcct cgacttgaag attttgctcc attagttcgg
     1141 gtagcttcaa cataatgtat attaaaatcc ttatacaact cctctactaa agcactagaa
     1201 gaatttgata acataacata agctcctgta tcactcaatc tcttaaaggc atctcttaat
     1261 cttacttgat ctgcaaaaga gaatccctca tgagtataag acgtaaatgc actcgtctca
     1321 gacaatggaa tatatggagg gtcaaaatac acaaaatctc ctgttcgaac atctacaatt
     1381 gccttttcaa aatctcccac tttaatttct agttgattgt tatttatata aactgaaata
     1441 gcagatatca attcctcatc aacaatttta ggattcttat aacgtccata tggaacatta
     1501 aattgattct tagaattcac acgatataga ccattaaagt tcactctcaa catatataga
     1561 atacgtgcag ctctttgtac ttcggacatc atatctattc tttcatcacg atctgcagaa
     1621 cgtaaatcta aataatattc ttttgaatta tattcctgat gaactttcaa aatttcaatc
     1681 aattcttgag gattgtcctt aatttgttga tagcaattta ttagttcagc gttaaaatca
     1741 ttaataactg catctttagg agccaaatca aaaaataaag ctccacctcc aacaaaaggt
     1801 tcgaaatacc tgttataggt tttaggcatt aattctctaa taacaggcaa taattgtctt
     1861 ttaccacctg tccatttcgt gaacggttgt aaagtaactt tctttatttc ttttatcttc
     1921 atgcttttaa ttataccata gaaattaaaa cacaactctt gtattttcta ccacattact
     1981 aaattttcta ttttagaatt aaatcatatc aatatcaaaa aatgagtaag gcaaatctta
     2041 taaattccaa aattcacctc actctattaa attactcttt atatccttac ctgtctttct
     2101 tctgaccgct ggcctgtgac aaacatggta aaggttgctt tacagacatt tctgccctct
     2161 tgattggtga tatccacatc catcacacag gttgtacggc cttgatggac acattctcct
     2221 ttaatggtca atacatcgtc gagttttcct gccttaaggt agttgataga ggattggagt
     2281 gtcaccccat caagtcccag cgagataacc accaaaccac tgatttggtc acatagcgtg
     2341 aatagatagc caccatgggc attgccatag tagttgagcg acgagttcac tactttggtc
     2401 gtcaccacaa cgtgaccatc tctcattttt tcaatttcgt aattttcaaa ggcagatata
     2461 gcgtcaaaat gaaagtcttt catcgtttcc tcctgatatt tcaaacgaca ctatgatact
     2521 actttttata ggactgtgca aggagaaagc tcctagtcta gtgaaattcc gacatgttcc
     2581 acaaagcgca taaaggctgt ctgcccttgt tctgtctgct tgtagactcc tgcatcctca
     2641 agtacacgca caaagatagc acccacagag tccttgacga ttgcaagggc tttttcttta
     2701 tccgttaggt ctggatgttg ggatttgagt tggtctgccc actcctgatg ataatcggca
     2761 actgtaacag cttctcctac aagatagcta gcgacttgct ccacttcttc tttcagacgt
     2821 ggtggcaaga ttgccaagcc catgacctca atcaagccga tattttcctt cttgatatgt
     2881 tggacatcct tgtggggatg atagatgcca tcaggatgct ctgctgaagt ctgattgtct
     2941 cgcaagacca agtccaactc aaactgtcca tcacgcttac gcgctattgg tgtgatggtg
     3001 tgatgcggtg tcccgtctgt ctctgccaaa atctgcacac tagaatctga atactgacgc
     3061 cattcctgga aaatcttatc agctaagtta atcaaatcct ctttggaatc cgaagtcaag
     3121 cgtaggacag acattggcca cttgataatt ccagccttga cctgctcaaa accagcaaat
     3181 cggaaggtct tttgcaaggg agccaattcc ataggaaata cgtgacggcc tccctgataa
     3241 tgatcatgag ttagaataga gccccccaca atcggcaggt cggcattaga tccagcaaaa
     3301 tatcctggaa actgctctac gatagccaac agacgttcaa aactctgacg actaatggcc
     3361 atgggacgat gctggccatc taaaaagata caatgctcat taaagtaagc atagggcgaa
     3421 tactggaacc cccattcctg accaaccatt tcaaaacgga taatccggtg gttgctgcga
     3481 gctgggtggt tgacccgacc atggtaaccc tcattctcta aacaaagttg acactgagga
     3541 taattactag cttgcaccaa cttggctacc gcaatctctt tcggatcctt ttcaggctta
     3601 gagagattga tggtaatttc aagttctcca taatcagatg gaatacgata agcgatattt
     3661 ttagcaatgg ccttgagttt gatgtagtca tttttccgac tgagttgata aaaatcctct
     3721 atcgcttgct caggagagta ggcataggct tcccagaagt cacgattgac ctgactcgga
     3781 caaggagtca ccaagtccat cagttcagta ccaaggattt cacgcgcagt ctgactatcc
     3841 tcaatcgtct ctaatcgaac ggcttcctca actagctggt ccttgaggtc aatcaattta
     3901 tccagattgg tctcaacttc caaaacacct tctcccactc gtgccaaaac acgattggtc
     3961 aggtagattc gatccatttc ctcaaatgaa ctttcagaaa tgacatgtgt tacaaattta
     4021 tctactaagg tcactataga gcctcctttt gactagtcaa ggacgcgagt gccacctgca
     4081 acttcagcga tatagaagct tggagcgtat ccaactactt cctcgtagtg tttgcctaca
     4141 gcttccttaa aggcctcaac agtatctttt tgaaccaagg caatggcaca gccaccaaaa
     4201 ccagcccctg tcatacgagc accgagaact ccttcttgtg cccaagctgt gtgaacaaga
     4261 gtatccaatt ccaaaccagt tacttcataa tcatgctcca gagaaacgtg tgacgcattc
     4321 atcaaacgac caaatgtttc caaatcacct gcttgaaggg ctgcttgagc tttaagggta
     4381 cgttggtttt caagcacagc atggcgagca cgtttcaaac gattttcatc tttaatcaga
     4441 tagctatatt ggtcaacggc ccactcgtcc aattcaccca gggtctgaat atccaaggca
     4501 acttgcaatt cttccactgc tttttcacac tcagcacgac gttcattgta tttagagtcc
     4561 gccaattcac ggcgtttgtt ggtgttcatg ataacaacga cattgtcctt caaatcaagt
     4621 ggcaccaagt cgtattctaa agtattggta tctaagtaaa tagcacgttg gtcagccccc
     4681 ataccaatag caaattggtc cataatgcca gaatttactc cgataaagtt attttctgtt
     4741 tgttttccga ttttaaccaa atcgagacgc tctaatttta aatcaaagag atgctctgcc
     4801 actacccctg tcaagagttc aagggatgct gaagaagaca aaccagcacc atttgggata
     4861 ttcccataaa cataaaaatc aaaacctttg tcaatcacgt gcccagcttc ttgcaagaaa
     4921 tgaaggacac cttttggata attggtccag ttgtgctctt tttcaaactt gaggtcagcg
     4981 agaggcactt cgataatgcc cttgtcctca aagttagctg agtagaaacg caagacttgg
     5041 tcatcacgct tgcgagctgc accataagtt cctaaggaaa tagcagctgg aaaaacgtgc
     5101 ccaccgttgt agtctgtgtg ttcaccaatc aaattaatac ggcctggtga aaagaaagtt
     5161 tggtctgctt cttgaccaaa aacagcaaga aagtctttgc gaagagtttc agcagtaaga
     5221 tgttgtgtca tatgatttct cctttgactg tccgttaagc caccttaacg tgtaatcgtt
     5281 ttcttctatt gtatccaagg gatttcccaa ggtcaatcct ttttactaaa attttactaa
     5341 actatcagta aaaagcagaa aaatatgata tactaaccta atcaaggagg atttatggct
     5401 accttaaaag acattgcaca gctagcctct gtctctatcg cgaccgtatc ccgcgtcctc
     5461 aaccgcgacc agagcctatc tgttacagaa gaaaccagac accgtatttt aaccgttgct
     5521 gaagagctgg gctacaccaa gcacctcaag acaggcgagt cccataaacc caagcaaaag
     5581 attgccatta ttcaatgggt cagcgaacaa ggggagctgg acgacctcta ctactaccag
     5641 attcgcctag gaatagaaaa aagagcccaa gagttggact acgatatctt gcgctatttt
     5701 aatgaccacc cttttaccct aagcgaggaa gtgattggga ttctctgcat cggaaagttt
     5761 agtcgagctc agatttctgc ctttgaagaa taccaaaagc ctcttgtatt tctagacagc
     5821 gatacacttt ccctgggaca tacctgtatt attacggact tttacactgc catgaaacag
     5881 gttgtcgatt atttcctcag tcaagggatg gaccgtatcg ggattctaac aggccttgaa
     5941 gaaacaactg accaagaaga aatcattcag gacaagcgtc tagaaaactt caaaaactac
     6001 agtcaagcga ggggaatcta tcatgatgaa ctggtctttc aaggaagatt tactgcccag
     6061 tctggctatg acttaatgaa ggaggccatt cagagcttgg gagaccaact tccgccagca
     6121 tttttcgcag ccagcgatag tttagctatc ggtgccctcc gtgccctcca agaagctgga
     6181 atcaacctgc cagatcgcgt cagcctcatt tcctataacg acactagtct gaccaaacag
     6241 gtctatcctc ccctctctag tattacagtt tatactgaag aaatgggccg agcaggtatg
     6301 gatattctta acaaggaagt cctccacggt cggaaaatcc ctagcctgac catgctggga
     6361 accagactga cattaagaga aagtacccta aatcaagaat aggataacat aaaaaacgaa
     6421 taggagtttt agaactccta ttcgtttttt attcgattac aatcatagac ttaatggtct
     6481 tacgttcatc catatctttg taggcttggt cgatatcttc cagtttataa cttgaagtaa
     6541 agacgcgacc tggattgata tcaccatcaa ggacggcttt tagtaaaaat tgcttatcgt
     6601 atgttgtagc agaagctgcc ccacctgcta cagagatatt ttgcataaat gtcgaaccaa
     6661 gagcacgatt attatagtgt gggactccta caaagcccat acgccctcca ttatgaagaa
     6721 cacctagcgc ctgttctata gcaacctccg taccaacaca ttcaagtgct gcatctgctc
     6781 ctccaccgag gatttcacgc accttggtaa ttccttcttg accacgttct gcaacaacag
     6841 ctgtcgcacc tgactccata gccatctttt gacggtcttc atgacggctc ataaggataa
     6901 tttgtgatgc tccacgcatc ttagccgcga tgacagcaca ttgaccaaca gccccatcac
     6961 cgataacaac aaccttgtcc cctttttgaa catttgcaac acgcgccgca tgatagcctg
     7021 tcggcatgac atctgcaaga gtcaaaaggg acttgagcat cccttctgta tagtcagaag
     7081 gttgaccagg gattttaacc agcgcccagt ttgcatagtg gaagcgaata tattctgcct
     7141 gaaaatcacc ccccaaatta ttgccaatat gattgtcgca agaaccgtca aatccagcaa
     7201 gacaggcatc acactcacca catccatgtg taaaagggac aatcacaaaa tcacctggtt
     7261 tcaccgtcgt aatggcttcc ccagcttctt caacaatccc aatcgcttcg tgtccactat
     7321 ttttgtgtcc agctttcgtt tctggattac ggtacctcca taaatctgaa ccacaaacgc
     7381 acgcacgaac cacacgaata atcacatcat ccgcttctat tatttgcgga cgttcaatgc
     7441 tagcaagtcc aacctgacct gcctttgtat atactgctga tttcatttaa aattttcctt
     7501 ccttataaag tttaattttg agatttaaac gatttaaagc tgtctgtagc tgagacaagc
     7561 gctcctctaa tttttgcttt tcctcttcta aaataccaag cctctcctct ctcgtttcat
     7621 ctcccttttg gtagagcgac atatagtcaa ctaaactatc tacagagaca cacgccgaac
     7681 gaaaacactt aataaattcc agcgcttcga tatcctgatc ttgaaaatca cgaatcccag
     7741 tagcagtacg agtaatcggt ggcacaagac caacccgttc ataataccga atcgtatccg
     7801 ctgaaattcc caacaaatca ctggcagatt taatattcac acgctaacct cctctcattt
     7861 cattcactga attcacgcta ttctaactca cttatcttat tatataactt tgagttgact
     7921 ccaagtcaag cattttgtat ctaatcgaaa aatttttatc attaacaatc atatatctga
     7981 agtttataca atgctcttct cttaccaatt tttgaaatat ttaaataata aagaaattac
     8041 tctattccaa ataagatagt acccaagtat ttttcacttt tttctaatgt tgatgctcat
     8101 aactccgttc caagtcacac acctttcgct tatgggtttg gtgagtttct aggtcagcat
     8161 caatttcaat ggtaatattt tgaaaaccac aatcttttag gaaaattcga atagactctt
     8221 tacaagtttc catatattcc atttctttta gacaaacatg gacaatggca tttttttcca
     8281 aagcatccat agtccagaga ttaagctgat taaggctggc cacattgtcc aatcgctcca
     8341 ggccactctt tacttgcttg atatcaagac cttctggcac agcatccaag aaaatcttga
     8401 gtgtagacca aaaacgtgga agggcttttg aaagaataaa gaaagaaatg acaagggaca
     8461 aaagaggatc taggatatac cagtccgtaa atcgaagaac aatcgccatc aggataacag
     8521 ctacccaccc tagcgtatct tccagaaaat gcagactcag aatagactca ttctttgtct
     8581 ttcccttacc aaccaccaga ctcgctaaca gattgatagt aatcgcaata attcctaacc
     8641 agagaatccc ctcatcattg actggttgcg gatgcaaaat cttcgtgaca ttttccaaaa
     8701 tgactagaac agagcccgtt acgagaatca cagctgttac caaggctcct agcaggctaa
     8761 accgcttata gcccaaggtg tactgattgt cttcttcacg attggagatt gtttctagaa
     8821 aagctgacat tccaattgca atcgcatctc ccaagtcatg cacagagtca gcaagaacag
     8881 cgctagaacc aaatactcca cctgcaataa actcaacaat ggcataagtc aaatttaaga
     8941 aaaaagccac ccaaacagca tattttgcct tcatatttct cattcctttg ttataataga
     9001 tttatgaaca ccttgttcgt tatcattatc cactaaaact caaagaaagg atagaagcaa
     9061 aacgtcagct ttattcagtt ctgaacaatt tgccttaagt gtccatatga ctaacattga
     9121 ccgccgtatc agcaaaacca aaaaagccat ctatcaagct tttatacaac ttttgaatgc
     9181 taagggctac gaggccacta ctgttcagga tatcattgat ctcgcagatg tgggacgatc
     9241 caccttttac tgtcactatg agagtaaaga gcttcttctg gatcagctct gccgctacct
     9301 cttccatcat ctttttgaaa gagagcaagc catttcaacc gaagactacc tcgcccacct
     9361 ctttctgcat tttcagaaaa accaagacca tatcgccagt ctactattct ccaaaaatga
     9421 ctatttcctc cgtcaactac agagagagct tgagcaccac gtctatccca tgctggctgc
     9481 tgatttgaag caaactcacc caagtctacc tacttcttac ctccaacatt ttgtcgtaac
     9541 taactttatc gaaacattga cctggtggct caaaaaaggt caagatttca cagaccagga
     9601 agttgtccaa ttttatctag accttctcat tcctaaaaat tgaatataga gtaaaacttc
     9661 agttgtctta tttctaggtt actgagtttt ttatcttttc aacaacaaaa gaggacccgc
     9721 cgatcctctt tttcatacta taaatccttg attatcaact atatctgttt taatcgaaat
     9781 ctcaaaacag cactttcaaa catcttttcc tagttaagta aatcagtatt ctgcttagct
     9841 gccttgctcc attgatacca accaactaga ctgttaatga gataaattag atatttccct
     9901 tgaatttgca ggctttctcc ccaccagaga tagattgaaa agacattggt agccgcccag
     9961 aatatccact gttcacggta aacagctgtc atgaggattt gccctacccc attggttgca
    10021 tctgtgattg aatcacgata gggacgattg gcaccaatag actgataaat gaagccaaag
    10081 gccaaccacc aaagcacact aatggaaaga tactttgtcc agcccttgcc gtccagttta
    10141 cgcgcgacaa actcctgctt ttccttctta aactgtgcct gataaatcca aactagaagt
    10201 ccaattggct gcatgactgt gaagtaaagt gtcgtcagca cctcaccata aaagcctttc
    10261 tgtagggcca aaataaggta aataacagag ttaatcaagc caaaaagata attacttgct
    10321 cgaccttccg atacaaagat tacacagata atccctgtca agctacaaat catcccaatc
    10381 cagtcaacaa tacgatgttc gtaaaccaac tccagccaga gaggaaaact tcctaaaacc
    10441 agcaaataaa tccactgggc aaaactacga tgggcaaaga ggtcatccca gatagccttc
    10501 atagttcctg aaaatcctaa atcagccata gccgcaacca tacgacggta accacctgac
    10561 atttcaccta gggttgtttt gatattttca attttctttt gcaaataagt atgcatcatt
    10621 tctccttttg tttttaaaga gccgtgtctg gatagacttt cggacgcaac gctctattag
    10681 ataatgaact gcctatacac aagatttcta accttagtcg acatgagctg aaacctctta
    10741 tttgttaagt agttcacaaa atattataca cctattttat gaatagtcaa ctgtctttac
    10801 agtaaaattt tagaaaatca tgaaaatttt ctctttcttt ccattttaag tgacattcag
    10861 tcattctcac atcaaaaaag cccagacgaa attgtctgag cattctttta tctagtcgtt
    10921 taaggaagtt gagttcagta tgtttaaagt ctctgtccca tcatttcttc aacaaacctt
    10981 gttcttggag aaactccttg gctacttgct ttgctgactt gccttcaaca ccgacttggt
    11041 agttgagctg gctcatctgg ctttctgtaa tcttaccagc caatgtatta agaactcttt
    11101 ccaactctgg gtgtttcttg agaagagctt ctttcatgag tggagcccct tgataaggtg
    11161 ggaagagttg cttgtcatct tccaagacct gtaaatcata acgctccaat tccgcatcag
    11221 tcgaataggc atccgtgatt tgaatatccc ctgactgaat tgcctgataa cgaagagctg
    11281 gctgcatagt cgccacattg agattgagac catacattga ttgcaagccc ttatttccat
    11341 cttcacggtc gttaaactca agcgtaaagc ctgccttcag ctgcccttcc acttttttca
    11401 aatccgaaat ggtcttcaag ccatattctt gagcaatctt tttcggaaca gctacagcat
    11461 aggtattttg ataagacata ggtttgagat aggctagatg atcctgcttg gcaatgccat
    11521 cacgcgccac ttgataaacc tgctctggct catgactcac cttgggtgat ggttgaagca
    11581 aactttcagt caccgtaccg gtaaattcag gataaatgtc aatatcacct tttttcagag
    11641 cttcataaag gaaacttgtc gtcccaaaat tcggtttaac agtcgcagtc atgctggtat
    11701 tttcttcaat cagcaattta tacatattag ccaaaatttc tggttctggg cctattttcc
    11761 cagcaataat caagttttcc ttctcttttt gaaccaaaag agctggacta taagacagac
    11821 ccagtaataa agccaccaag gcaaaaccag agaaaatcgt ccgtaatttt gctttttcca
    11881 ttacttttag taggaagtta aaggcaatag ctagcactgc agaagaaagt gccccaatca
    11941 aaatcagact ggcattatta cggtcaattc ccaaaaggat aaaggaacct agtccccctg
    12001 ccccaatcaa ggtcgccaag gtagccgtac cgataatcaa gaccgctgcc gtccgaattc
    12061 cagacatgat aacaggcatg gcaagtggaa tttcaaactt cttgagacgc tcccatctgg
    12121 tcatcccaaa ggcaatccca gcctcttgca gactcggatc aattcccttc agcccagtaa
    12181 tagtattttg taaaatcgga aaaatcgcat aaatcactag agctgtcaaa gccggcaagg
    12241 tcccaattcc catcaaaggg ataaagagcc ccaacaaggc cagagacggg atggtctgga
    12301 aaatacctgc aatctgcaag acccagtcgg ccagcttctc atgatagcga agaaaaacag
    12361 ccaagggaat cgcaagcaaa atagctagta acaaggtcaa aagtgacaac tgcaaatgtt
    12421 gagatagagc tgtcaaccaa tcactaaaac gatcctgaaa agttgcaatt aaattagtca
    12481 tgaacactac ctccaaacaa gtctgctaca aagtctgttg caggcacttt taaaattgtc
    12541 tcgggattcg ctacctggcg aatttctcca tcctgcaaga cagcaatacg gtccgccaac
    12601 ttcaaggctt catccgtatc atgggttaca aaaatcgttg tcatcccaaa ctctttatgc
    12661 aattcttttg tcagaacctg caactgtttt ctcgaaatag catccaaggc cgaaaagggt
    12721 tcatccatga ggaaaatctt gggctgacca atcatagctc ggacaatacc gacccgttgc
    12781 tgttctccac cagataattc actaggtaag cgatgcccat actcagctac tggtaaacca
    12841 accttagcca aaagctcttc tgttttcttc gtaatttctt ccttgctcca ccccttcatt
    12901 tcaggaatga gagcaatatt ttccgcaact gttagatttg gaaaaagagc aatagcctgt
    12961 aaaacataac cagtagaaag acgaagttca cgctcatcat agtctttgat gcgcttccca
    13021 tccatataaa tatttccatc agttggttcc aaaagacggt taatcatctt gagcatggtc
    13081 gtcttacctg acccagaagg ccctactaaa accataaatt ccccatcctc aatctgtaag
    13141 ttgacatctc tcaagacatc cttttctgtg tagcatagtg ctacattttt gtattcaatc
    13201 attctttgtc ctcatttaaa acttccctcg attggtcaag tcttctacct taggcatgac
    13261 ttccttatta tcccaatgct ccacaatttt cccgttcttt aaacggaaga tatcgtactg
    13321 ggcataagca acgccatcaa tctgagtctg accatagcta accacatagt ttccttgtcc
    13381 taagagttgg aaaacaaagt caaaagtgac actatattca gccacatagt ttttataagc
    13441 agcacttcct tgtccaatat catgattatg ctgaatcaaa tcgtctgcca cataatcact
    13501 ccactgctct agctccccat tttggaaaat ttctgtcaag aaacggcgaa ccagcttttt
    13561 attttctgct ttcttgtcca aatccttgat ttcaaaatct ccaaaaattt gatctagttg
    13621 gtcattttca ggtgttcgat agtagtcaat gacatcccaa tgctcaacaa tacgaccatt
    13681 ctcatccgca cggaaagtat ccgtcgtcac ccattgagct tctccaccat tcagatattg
    13741 atgaacatga acaaagacca gattgccatc ctcaatggtg cggacaatct taatctgacg
    13801 ctctggatga cgctcaaaga aatctgcaaa gaaggctgca aatccttctt tcccgtcagg
    13861 aacacctgtc gaatgttgga tataggtatc ccctacagac tgggcttgag cctcagcaac
    13921 tcgtccgtct tgaatggcat ggatgtatag gttgtgagca tttttcactt gttgtgacat
    13981 attctaaacc tcatttccct tctctttcag attcgccaaa attctttctt gaaaaccttc
    14041 aaattggtga atttcttcct ctgaaaatcc tttgtaaaag atagtatcca atttctgact
    14101 gacacgatgc cccacttctt tctgggactt gcctaactcc gttaaaacta aatacttctt
    14161 acgcttgtct tttccacacg gactaacaat tacaagcttt tgttcctcta gcttttttat
    14221 catagtcgtc agcgtattat tcgcaagtcc agtcgcaagc gcgatatctg tcgcagttgc
    14281 gcagccagtt tcactattcc ataaaaccgc taaaatcttg ccctgttcac ccctataaag
    14341 agcctcagga tcttgactca gtaacttttg aaaaatccgc ccattcaaca aacgaatatg
    14401 atgggctagc aaatgaccat ctttcataac acctccaatt tatttcgata tcgaaatgaa
    14461 taaaacaatt gtaacactca tcgttctaac tgtcaactat ttcgatttag aaataatttt
    14521 tgataattat ccacaccacc atactccggc tcaactaact tttaacgaga gtttctaaac
    14581 tccttcgtcc tccagtctac aaaagccttc catccgtact atcctatatt ttatgagggg
    14641 acacattttt cctatcagag catttatttt aaagatagaa gtaaatcata attgcttcca
    14701 tctgttcttt tatagtatat tgaagttgga ctagggcact gtatcttcta aaacattgat
    14761 agaaagcgat ttgaatttcc caatcaattt gctcgtattt atagcatttt gaaactagaa
    14821 taggacgcca tgactgctaa aagatttcta taaattcatt tgattttcct aatcaatttg
    14881 ttcatgtctt atttcatctc gctataattt caccttaccc tatctttttc gtagcaccct
    14941 tcaaacagcc tatcccctac cgtttgacga ttcctcactt cactccactt ccattacaga
    15001 agtttcttca ctactatggt ctcggctgac ttctcatgat tccttgttac tactatttga
    15061 acgctcacga gataggtctt ataaaaaatg ctttgatcca caatggaatc aaagcatttt
    15121 aaagagttcc tcatacataa gcgcagaagt cgcagttcct ctgtacttgg cttcttctct
    15181 tttgataaag ctagccaagt tgagcaactc aggtgctgga tgtttgggat ttaggagcaa
    15241 ttcacgattg accaggcctg agagacgaac tgcctgcaat tgctcatttg tagtaggcag
    15301 ttttttagta gtctctagga gagcagcaac taaatcttca ctcaaatcat gtcgagcatg
    15361 attgtaaaga tcttttataa ggctttctag gtttggttct accatcccta ccacctccct
    15421 tatggtttaa taatgtttaa tcaaatcaac cgttgaacga tccaatttct tcaccaaggc
    15481 ttgtaagaaa gcttgcgctt ctaggaagtc atccattgca tagagggttt ggtgagaatg
    15541 gatataacga gcgcagacac cgatagttgt tgatgggaca ccaccatttt tcagatgagc
    15601 tgcacctgca tctgttccgc ctttaccaca gtagtattgg tacttgatac cagcttcttc
    15661 agccgttgtc aaaaggaaat ccttcatccc tggaagaagc aagtgacctg gatcatagaa
    15721 acgaatcaag gttccatctc caatcttgcc ttgaccaccg tagacatcac ctgctggtga
    15781 gcaatcaact gcgaggaaga cttctgggtc aaacttggtt gtagaggtat gagcgccacg
    15841 cagaccaact tcttcttgga cgttagaacc cagatagagt tcattgccga gtttttgacc
    15901 tgataaagct tcagctagct cgcttaccat gaggacaccg tagcggttat cccaagcttt
    15961 tgagatgata tttttttcat tggctgtcaa aattgcagaa ctatctggta cgatggtatc
    16021 accaggacgg atgccaaaac tttctgcctc agccttgtcc gcaaaaccac catcaaaaac
    16081 gatatcggca atggctggca tggttggtcc cccctttcca cgagtcaaat gcggaggaac
    16141 agaacctgaa atcacaggaa tttcatgacc atcacgagtc aagagtttga aacgttggct
    16201 gctaaccacc atggggttcc agccaccgat ttctacgaca cggaaggtac catctagctt
    16261 gatttcgctg accataaaac caacttcgtc catatgagaa gcgaccaaga cgcgcggtgc
    16321 atccacagct tctgaatgtt tgataccaaa aataccaccc aagccatctg tcaccacttc
    16381 atccacatgc ggtgtcaact tttcacgaag ataagcacgg acaggcgctt catgacctga
    16441 gactgcagca agttctgtta cttctttaat ttttgaaaat aatgttgtca tttcagttcc
    16501 ttctttcttt catccatttt accacttttt ataggagaag gatagtggga aggtggattt
    16561 ctaagttagt atcttagtcc tgctctatct tagaaaagga tagtattctc ttgcatgtag
    16621 tgcaaaatct agtaaacatt ccaaaattaa ctcgaatatt tatttccaaa caaaaaaaca
    16681 atacaccatc aaagttgttt agatttttca tgaaatttac agaaaatagt tgacttccct
    16741 ttcttctttc tttaaat
//
LOCUS       DAVKQL010000046        16711 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVKQL010000046 DAVKQL010000000
VERSION     DAVKQL010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16711
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.046"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            260..1111
                     /locus_tag="VW561_001519"
     CDS             260..1111
                     /locus_tag="VW561_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9943758.1"
                     /translation="MRWDYGQIFKEIRKSKGLTQQDVCGQVIHRTTLTNIEHGKVIPS
                     FENMVFLLEQIDMSLAEFKYIWNEYHPSKRRDIIVESQNPSTFQDTRKMVELTEKCQK
                     YLKTHHDVPIQNIYRHTKIVTELRTKGFKNNHVLKDLSEEIWDYLEPMDTWYISDLKL
                     LGTILFFFPSENLPLLIDRIMKTIEKYKYFRETKVFLSSFLANLSTVYFQHHLFKECE
                     TITLQLLVLAEELKIYDILGFSQVRLGILQHNSDLIDKGITLLRLTKEEALVEILEKE
                     INDFSNL"
     gene            complement(1333..1683)
                     /locus_tag="VW561_001520"
     CDS             complement(1333..1683)
                     /locus_tag="VW561_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943759.1"
                     /translation="MEHLFKMIILLPCFYFFSWIEKANRDSKIFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLLFNIVLRNVADIKSWGVWLLLLLIAFASDWLAYVFFKKMFRLRQE
                     LGKSKGVHKGRCRN"
     gene            complement(1823..2158)
                     /locus_tag="VW561_001521"
     CDS             complement(1823..2158)
                     /locus_tag="VW561_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943760.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(2249..3559)
                     /gene="der"
                     /locus_tag="VW561_001522"
     CDS             complement(2249..3559)
                     /gene="der"
                     /locus_tag="VW561_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEU9943761.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(3573..4286)
                     /locus_tag="VW561_001523"
     CDS             complement(3573..4286)
                     /locus_tag="VW561_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEU9943762.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     ALLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYPVFGMAL
                     GVPNQTHDVKPRLPLDNVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(4283..5179)
                     /gene="dnaI"
                     /locus_tag="VW561_001524"
     CDS             complement(4283..5179)
                     /gene="dnaI"
                     /locus_tag="VW561_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEU9943763.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTVWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(5180..6349)
                     /locus_tag="VW561_001525"
     CDS             complement(5180..6349)
                     /locus_tag="VW561_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEU9943764.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLGHPVYRRLLEKKIGDVAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKEATIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEEAVLRIRDR
                     GQKAKTQKQNQTAPEKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(6350..6823)
                     /gene="nrdR"
                     /locus_tag="VW561_001526"
     CDS             complement(6350..6823)
                     /gene="nrdR"
                     /locus_tag="VW561_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEU9943765.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            6963..7328
                     /locus_tag="VW561_001527"
     CDS             6963..7328
                     /locus_tag="VW561_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9943766.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            7333..8028
                     /locus_tag="VW561_001528"
     CDS             7333..8028
                     /locus_tag="VW561_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9943767.1"
                     /translation="MSLLVFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            8045..8812
                     /locus_tag="VW561_001529"
     CDS             8045..8812
                     /locus_tag="VW561_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943768.1"
                     /translation="MFWNLVRYEFKNVNKWYLALYAAVLVLSALVGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTSVLLLCIYIILLSTNSNEVQLSTLLSFVGEHFTGFSLTVLSFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVVGFIEVFFNTSTNFYINSLAGF
                     NDNFYMGASIGIVEELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(9225..10424)
                     /locus_tag="VW561_001530"
     CDS             complement(9225..10424)
                     /locus_tag="VW561_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9943769.1"
                     /translation="MRNMWVVIKETYLRHVKSWSFFFMVISPFLFLGISVGIGHIQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILVHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGSFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAEGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(10421..11314)
                     /locus_tag="VW561_001531"
     CDS             complement(10421..11314)
                     /locus_tag="VW561_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9943770.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            11430..11510
                     /locus_tag="VW561_001532"
                     /pseudo
     CDS             11430..11510
                     /locus_tag="VW561_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            11995..12208
                     /locus_tag="VW561_001533"
                     /pseudo
     CDS             11995..12208
                     /locus_tag="VW561_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            12623..13240
                     /locus_tag="VW561_001534"
     CDS             12623..13240
                     /locus_tag="VW561_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEU9943771.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(13560..14447)
                     /locus_tag="VW561_001535"
     CDS             complement(13560..14447)
                     /locus_tag="VW561_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9943772.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEMVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(14596..16479)
                     /locus_tag="VW561_001536"
     CDS             complement(14596..16479)
                     /locus_tag="VW561_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9943773.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEAAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYALADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYASVAPVATGSVSKGDAVIE
                     VKI"
BASE COUNT         5010 a         3593 c         3031 g         5077 t
ORIGIN      
        1 taagtttggc tataataaaa ctacaaaaaa taagtatgtc aaacttaact aaagaatata
       61 aataacacat caattagtca ctcccaaaac catatatcaa accatggtat taaaggctcc
      121 caatcattac aatatataac aaaattaagt gaatcttcta tttccatgac ttttccctcc
      181 tttttgatat aataatacca cctttttcta cgttttttat aaatgtagaa aatattcaca
      241 tttacctagg aggaacaata tgagatggga ttatggacag atttttaaag aaattcgaaa
      301 gtcaaaagga ttgacccaac aagatgtatg tggacaagtc atacatcgga caactctaac
      361 aaatattgaa cacggtaaag ttattcccag ttttgaaaac atggtatttc ttcttgaaca
      421 aattgatatg agcttggcag aattcaagta tatatggaac gaataccatc ctagtaaaag
      481 gcgagatatt attgtagaga gccaaaatcc gtctactttt caagatacta gaaaaatggt
      541 tgaactcact gagaaatgtc aaaaatatct taagacacat cacgatgttc ctattcaaaa
      601 tatctatcgt catacaaaaa ttgtcacaga gttacgaact aaaggattca aaaacaacca
      661 cgtcttgaaa gatttgtctg aagaaatttg ggactatctt gaacctatgg atacatggta
      721 cattagtgat ttgaaattgc ttggaaccat tctctttttc tttccttctg aaaatcttcc
      781 ccttcttatt gatagaatta tgaaaaccat cgagaaatat aaatacttcc gagaaacaaa
      841 agtattttta tcatcttttt tagccaatct ctccactgta tattttcaac atcatttatt
      901 caaagaatgt gaaacaatca ccctacaact tttggtatta gcagaagaat taaaaatata
      961 cgatattctt ggattctctc aagtacgctt gggaatacta caacataact ccgatttgat
     1021 tgataaagga ataactttat tacgactaac caaagaagag gcattagttg aaatactcga
     1081 aaaagaaatt aatgattttt caaacctata atttgtaata actctcatca ttttattata
     1141 gtagattgta atagaatatg aacgaatcaa ttaggggatc taaagcattt tataccaatg
     1201 ttttataagt tgtattgtat tattccatat tcaatccact atacaagatg aatatttctc
     1261 tcaagtttca taatcaaaat aaaaatgact gtatctatag ctccataaac aaatcataga
     1321 tacagtcatt ttttagttcc tacaccttcc tttatgcaca cctttagact tccctagttc
     1381 ctgtctcaag cgaaacattt ttttgaaaaa gacataggct aaccaatcac tagcaaaagc
     1441 gataagtagt aataatagcc aaacacccca agacttgatg tctgccacat ttctcaagac
     1501 gatattgaac aacaggaccg aaaaaactgt ccaagccaga ctaaaaagag catagagggt
     1561 gatgtaaatc cagtaaaaat agtaaaaaat tgggaaaatc ttactatctc tgtttgcctt
     1621 ttcaatccag ctgaaaaagt aaaaacaggg taataagatt atcattttaa acaagtgttc
     1681 catcacaagc ctcctttttt aaattgtttt ctttagtttc gttctattaa atgttattac
     1741 caatataaac cttggaatag cgtgtaataa atgattttag aggtaccata agccacctcc
     1801 tacaaataga aaccgatata aatcaatgcc ttccaccctt agacttccct agttcctgtc
     1861 tcaagcgaaa catttctttg aaacaggaat aagttaacca attcatacca atagctagca
     1921 gaataaaaag aaaccaaatg ccccataact tgatatctgt cacatttctc aagacggtat
     1981 tgaaaaacag aactgaaaca actgtccaag caaggctaaa aagagaatag aaggggatgt
     2041 aaaaccagta aaaataataa aaaattggaa aaaacttact atttctgttg gccttctcaa
     2101 tccagttatc aaaataaaag tacggtgcta aaagtaagaa tttaaacaaa tgttccatca
     2161 ccgacatcac cctccttctt ttgatagcgt tttctattat tttattatat caaaaaaatc
     2221 cggaactgtc attccagatt ctactttttt atttgcgttt tcttgcgatg agatgaatcg
     2281 gtgttccctc aaaaacaaag gccttgcgga tttgattttc caagaaacgc aggtaagaaa
     2341 agtgcatgag ttcttcttca ttgacaaaga tgacaaaggt tggtggtttg gttgccactt
     2401 gggtcgcata gaaaatcttg agacgttttc ctttgtctgt cggtgttggg ttgatggcaa
     2461 tggcatccat gatgacatcg ttcaagacag ctgatggaat acgtgtgttt tgactttcgc
     2521 tgatttgctt aatcatctca ggaagtttgt ggagacgttg cttggttaaa gctgatacaa
     2581 agataatcgg tgcgtaaggc aggtattgga actgctcacg gatatcttct tcccagtttt
     2641 tcatagtgtg gttatctttt tcaagcgtat cccacttgtt gaccacgata atcatccctt
     2701 taccagcttc atgggcaaat cctgcgatac gcttgtcgta ctcacgaatg ccttcttccg
     2761 cattgatgac catcaagacc acatctgaac ggtcaatagc acgcatggca cgcataacag
     2821 agtatttctc agtattttca taaaccttac cagacttacg cataccagcc gtatcaatca
     2881 tggtaaactc ttgaccatct gtatctgtaa agtgggtatc aatggcatca cgagttgttc
     2941 cagcaacagg actagcaata acacggtctt ctcccaagat agcattgatc aagcttgatt
     3001 ttccaacgtt aggacgacca atcaggctaa acttaatgac atctggattt tcttcctcat
     3061 attcatttgg aagattttct acgatagcat ctagcacatc ccctgtaccg attccatgga
     3121 cagatgagat aggcagtggt tcacccaaac cgagagcata gaaatcatag atatcatttc
     3181 tcatctcagg gttgtccacc ttgttgactg cgaggataac tggtttgtgg gtcttataaa
     3241 gcttacgagc tacgtattcg tctgcatcag taattccttc cttaccagac acgacaaaaa
     3301 cgataacatc tgcttcttcc atggcaattt ctgcctggtg cttgatttgt tccatgaaag
     3361 gagcatcgac atcatcaatt cctcctgtat caatcatact aaaagagcga ttgagccact
     3421 cacccgttgc atagatacgg tcacgtgtca ctccttcgac atcttctaca atggagattc
     3481 gctcaccagc gatccgatta aatagggttg atttcccaac attgggacgt cctacaatgg
     3541 caatagttgg tagggccata atttctcact ttctacaata acttcttctg ttcaagattt
     3601 tttctagttg agcttggttc agcttgacca aactgttctg ctaggcgctg actccagctt
     3661 gtggtcgcac gcgctccagc atagtcagcc tgtacacggt cataagcttg gattgcctca
     3721 gttgactgtt cttggtattc ttcctcaaag actacattat ctagtggcag tcttggtttc
     3781 acatcatgag tttgatttgg aacacctagt gccatcccaa agacaggata ggtgtagtca
     3841 ggcaggttaa agagttctgc cacttcttct gacttgtacc gtaccaagcc aataatcaca
     3901 ccaccataac ccaaactttc agctgccagc agggcatttt gtccagccaa agccgcatcc
     3961 accgaactaa tcaagagagc ttctacacct tggggttgga aggtgtcggt atgaagtcgg
     4021 gctccctttt ctgctcggtt caaatctccg acaaagagaa ggaaaacagc agactggcga
     4081 atggcttctt gaggtaccaa ttcatacaag gcatctttct tctcttgact tcgtaccaca
     4141 atcacagagt aggattggaa attcttccaa gatgatgcca tctgggctgc tgtcaaaatc
     4201 tcatttaagt ctacttgggg aatttcttgc tctttaaacc tgcgcactga agtatgagcc
     4261 ttcatcaatt taatggtttc tgtcatcgac ggtttactcc ttctaaacga gtctcctcag
     4321 ccaaataacg gatgcgttcc atgacccgtc tggcttccca ggtttcgtca tttccatgtt
     4381 tcactttcgc aaaatgcttc tccaaatctt caaagttgaa gttggatgtg aaaaaggtcg
     4441 gtaaattttc ctgcatccga tattggagaa tgacctgcag gatttcgtca cgcacccaaa
     4501 cggttgattg ctcggcgcca atatcatcta aaatcaggac ctcagacagc ttaatctcat
     4561 ccaccaaggt cttaacattg ccatcactga tagcattttt gacatcaatg acaaagctag
     4621 gatagtggag gagagttgat gaaacaccac gtttttctga taaatcatga gctaaggccg
     4681 ccaccatgaa acttttaccc acaccaaagt ctccatataa gtaaagacct tttcgaatag
     4741 ctggatattg ctccacgaag gctagtagct tttcaaaaac tggtaagcgc cccaaatcat
     4801 ccaagtcaac ttgagccaaa ctagctttct tgagactggc tggtagattg attaacttga
     4861 gacggttctt aatagccgct tctttttcag ccgcgattag ctcaggagtt tcttcatatg
     4921 aaacatctgc atagccatga ttcttaacca aaatcggctt gtagcctttg gcaatataat
     4981 ccgtatcccc acggagaaac ttgtcacgct cggtgatgta ctgattaaac ttggagatac
     5041 tgcgatttaa ttcctctgga gttaaggatt cttgctggat aaaggccgca acatcagggt
     5101 ccttcatgat tttctggacc aaatcttgat aataaaaacg gctgggttga cgtttgagta
     5161 cgtctccgac actttccatc taatctcctc ctttttctaa tcgagctaat agttcttgct
     5221 tcttacgttc tagttccaga cgagtttcct cgctggtttc attcttatat tcaggattac
     5281 tccatttagg aacattggtt ttttctgggg cagtctgatt ctgtttttgt gtttttgctt
     5341 tctgccctcg atcacgaatt cgtaaaacgg cctcttctgc cgaatgaatc ttttgatagg
     5401 catagtcatt ggctaccttc atggcatatt tctcattgat atttgccgaa tccaccttat
     5461 taaaggtcaa taagagaata atattgatga cttcgtccag taagcccaag ccagccatct
     5521 gttgcaagag ttctctttct gtttgggtaa tggttccctt gcgtgtttgc ttgatttctg
     5581 ctaagaactg cagggcagtt ttacttttag cttctttgat aatggtcgct tccttaagac
     5641 taaagtcaga ggaaactggt ttttgagcaa ttttttcacg catgcgtttg gttgaaataa
     5701 cctgggaaac agctgttgac ttggccaatt gataggtttc aaaccaagtc catttcttct
     5761 cctcggcaat agcaaagagg tttaagacat cggactgctc atccgcaaaa cgaagtccat
     5821 ctcgagccat cagctggcga aaatgttcca agtcaaaatc attggccact ttcttcttga
     5881 gaccaaggtc ttcttgactg cctagttctg ccaattctgg aaagacttga ttgagtgaga
     5941 caggtatttc ttcaccatca gcactttcaa ctttcaaatc ctccacagct acatcgccaa
     6001 tctttttctc taagagtctg cgataaacag gatgccccaa gaagtcttga ctagatagag
     6061 gagcatggag ggctagctga taaacatcac ccttttgata gagggtcaag agattaaaag
     6121 cagataagat tttcaatgat tttatcagtc tatccatccc aaagttgaga tggttgagaa
     6181 tgcttgaaaa aagatattcc tttctaccat tatcccaaaa actgattgta taaagataaa
     6241 ggctcagtgc ctcctgaccg ataatcggga ggtagcactg taccagagat gaggtatctt
     6301 gcgacacccg attattcttt agataagaaa aacggtcaat tggcttcatt tatctttcct
     6361 ttttcttttt agaggactgg gtgatttgtt ggagcaagct ctctaactca ctgacatcct
     6421 taaaactacg atagacacta gcaaaacgta cataggtaat ctcgtccaat tcagccaact
     6481 cctccatgac gagtgaacca atgtcctcac tttgaatttc attttcattt cgaccacgga
     6541 gtttctgttc gatacgattg actaccatgt tgatttcatc acttgacaca ggacgtttct
     6601 gggctgagcg gataatccca ttaaagattt tatctctgga gaattgttcc cgtgtgccat
     6661 cttttttaac aaccactaag gttctttctt ctactcgttc gtaggttgta aaacggtgtt
     6721 ggcattcgtc gcactcacgt cttctacgaa tggtgttccc ttcttctgct tggcgactat
     6781 cgataacact tgacttggta gccccacatt ttggacaacg catcctttcc ctccttatcg
     6841 ttttcttttc attataccat tttttaagcg attcccaaaa caattcttct ttttgcttga
     6901 caagtttttt gttttgttgt attatttaat taagacaaca aggtaaaaga aaggagacta
     6961 agatgtcctg gacatttgac aacaaaaaac ccatctattt acagattatg gagaaaatca
     7021 agcttcagat tgtttcccat acactggaac ccaatcaaca acttccaacc gtgagggagc
     7081 tagctagcga ggctggtgtc aatcccaata ccatccaaag agccttatca gaccttgaac
     7141 gagaaggatt tgtctacagc aagcgaacaa ctggacgatt tgtgactaag gataaggagc
     7201 taatcgccca gtcacgcaaa caattatcag aagaagaatt ggaacacttc gtttcctcca
     7261 tgacccattt tggctatgaa aaagaagaac taccaggcgt agtcagtgat tatattaaag
     7321 gagtttaagc ctatgtcatt actagtattt gaaaatgtat ccaaatcata tggagcaaca
     7381 ccagcccttg aaaatgtttc tcttgacatt ccagctggaa aaattgtcgg ccttcttggg
     7441 ccaaacggct caggaaaaac aaccctgatt aaactaatta atggcctctt acaaccagat
     7501 caaggacgtg tcctcatcaa cgacatggac ccaagcccag caaccaaggc cgttgtagct
     7561 tatttgcctg atacgaccta tctcaatgag caaatgaagg tcaaagaagc cctaacctac
     7621 ttcaagacct tctataaaga tttcaatctt gaacgcgccc atcatctact tgcagacctg
     7681 ggcattgatg aaaatagtcg tctcaagaaa ctatcaaaag gaaacaaaga aaaggttcaa
     7741 ctgattttgg ttatgagccg tgatgctcgt ctctatgttt tggatgaacc cattggtggg
     7801 gtggatccag cagcacgtgc ttatatcctc aataccatta tcaacaacta ctcaccaact
     7861 tctaccgttt tgatttctac ccacttgatt tctgatatcg agccaatctt ggatgaaatt
     7921 gtcttcctaa aagacggaaa agtcgtccgt caaggaaatg tagatgatat tcgctacgag
     7981 tcaggtgaat ccattgacca actcttccgt caggaattta aggcctaagc aaaggagatt
     8041 atttatgttt tggaatttag ttcgctacga atttaaaaat gttaacaagt ggtatttagc
     8101 cctctacgca gccgtgctag tcctttctgc cctcgtcgga atacagacac aaggctttaa
     8161 aaatctacct taccaagaaa gtcaggctac tatgctactt tttctagcta cagtctttgg
     8221 tggcttgatg cttacacttg cgatttcaac cattttcttg attattaaac gcttcaaagg
     8281 tagtgtctac gaccgacaag gctatctgac tttgaccttg ccagtttctg agcaccatat
     8341 catcacagcc aaactaatcg gtgcctttat ctggtcattg attagcacct ctgtcttact
     8401 cctatgtatc tatattatct tattgtcaac aaattctaat gaagttcaac tttctactct
     8461 tctgtcattt gttggagaac attttaccgg ctttagtctt actgtcttat ccttcctact
     8521 aaatactatt tcaggaatcc tctgcatcta cttggccatt tccattggac aactgttcaa
     8581 tgaataccgt acagcactcg ctgttgcagc ctacattggt atccaaatcg tcgttggctt
     8641 tattgaagtc ttcttcaata ccagtactaa cttctatatt aattcacttg caggatttaa
     8701 tgataatttc tatatgggag caagcatagg cattgttgaa gaactcatat tcatagctat
     8761 cttttatctc ggaacctact acatcttgag aaataaggtt aatttgcttt aaataatttt
     8821 tacctagata tgtaacatac tcatagaaca aaagagacca ggcaaaaagt ctttaaaatt
     8881 agaaaacgca tagtatcagg tgttgaatat gtactgcccc ccaaaagtta gattttttct
     8941 gtctaacttt tggggggcag ttcataagaa ccttggtaat atgcgttttt tgtgagctga
     9001 cttatttcct ttcactatat cgcaaaatga aataagaacg gaacgatggg attttggaat
     9061 tcaaatcaat ttataagaat gttttagaag taatattatc ctattccaga ttcagttcac
     9121 tatacaattg agttttcaag caacctgttt acataatgtg tacataatta ggttcgtgat
     9181 tccacccttt tcacctttaa aaacctcgct ttcgcaaggc tcttctattt ataagataag
     9241 gcacgtttaa aggttttcca aatccctaaa tcatccgttt gaagaacgag actagcatac
     9301 atgcgtccga taaatcctgt tgctaccacc gcaaaaatca ctgtaatagc aagtgaaatc
     9361 catgcttccg ctccctccgc atagccatta atcgttcgaa acggcataaa gaaggtcgaa
     9421 ataaagggaa tataagaacc aatcttcaag aggagattgt caccagctgc acctagagct
     9481 gtcactccaa aaaaactacc cataatcaaa atcatcaaag gcgacaaggc tttccctgag
     9541 tcctcaggac gagaaaccat agatcctagg aaggctgcca agactacgta catgaaaaga
     9601 ctgatcaaaa taaagagcag ggtattcagt gagatagcat ctcccaagtg aaccaaaata
     9661 ccagactgag ccaagaatgg caaatcttta aagagcaaaa cggcagccag accacctaca
     9721 acatagatcc caatatgcgt taaaatcact agaaacagag ccatcatccg cgcatagaaa
     9781 tagtgacttg cccttatgct agaaaaaacg acttccataa ttttggtgcc tttttcactg
     9841 gcaacttcct gagctgttac acccgcatag gtaatcagaa tcatataaag aaagaatcct
     9901 aaggcacctg ctgcaattgt ttgaataaac tttttatttt ccttggcttc atcaatcttt
     9961 tctgtgaatt gaattgtctg cgctaagcgt ttttcctgct cttgagacaa ggaagcagtt
    10021 gaacgattaa gctgattttg cagttcattg agtgtacctg taacctcaaa tttaattcca
    10081 ttttcaagcg atgtttcgcc atgataaact gcctttagaa cactatcttc ttgatcaatg
    10141 gtcaaataac cttttaattt ttcttcttta attgcttctt tggcacttgc ttcgtcttta
    10201 tagtcgaagt taacaccatt tacattcttc agtccttctg ctacagatgg cactgttgtc
    10261 actactgcca ctttattatt tttagccata gaagaacctt ggatatgccc aattcctaca
    10321 gagattccta aaaagaggaa cggcgaaatc accataaaga agaaactcca tgacttgaca
    10381 tgtcgaagat aggtttcctt gattacaacc cacatatttc tcatacttcc actcctgatt
    10441 ctagtttaaa gatttcatcg atagttggcg cttgttggtc aaatgttgcg atatattgac
    10501 cttgagtcaa gattgggaag agttcccttc cagcgctctc atcctccaaa atcaatttcc
    10561 aactgccttg tttggtcaag ctcacctgtt tgacatgagg aagattttcc aattcttcct
    10621 tgcttcgttc acttgaaaca aagagacgcg ttttcccgta ttgattgcgg acatcctgaa
    10681 ctggtccgtg caagaccaca cggccatctc ggatcatcag aatatcgtca caaagttcct
    10741 caacattggt catgacatgg tcagaaaaga taatggttgc tccgcgctct ttttcctgaa
    10801 aaatgacttg tttgagcaat tctgtattaa ctgggtccaa tccactaaaa ggctcatcca
    10861 agataatcag gtctggttca tgaatcagag taataatgag ctgaatcttc tgctgatttc
    10921 cttttgacag actcttgatt ttatctgtca gctttccttt cacttccaac ctcttcatcc
    10981 attgagggag tttttctttg acttctttgg catccatgcc ttttagagtc gccaagtagc
    11041 gaacttgttc aagaactgtc aatttaggca tgagactgcg ttcttcaggc agataaccaa
    11101 tccgagcata ggtctcctga cgaatatcct gaccatccag accgatttct ccctgatatt
    11161 ctaggaattt taaaatacta tggaaaatcg ttgtttttcc agcaccattt tttccgacta
    11221 gtcccaaaat acgacctggt cgcgcttgaa agtcaatacc aaacaaaact tgcttggatc
    11281 caaaactttt ctctagactt cttacttcta gcatctttca cctccgaaat ttcttgcact
    11341 cattatactc ctttttgata gcctttacaa tgtttttgtc catttttaga agactattgc
    11401 tgtgtaaaat atggcctgga gcacttttat actcaatgaa aagcaaagag caaactagga
    11461 agctagccgt aggctgctca aagtacagct ttgaggttgc agataaaact gacgaagtca
    11521 gctcaaaaca ctgttttgag gttgtggata gaactgacga agtcagtaac tatatctacg
    11581 gcaaggcgaa gctgacgtgg tttgaagaga ttttcgaaga gtattagtga taaatccatt
    11641 atacagtagc aaacttaatt tataccttcc gctcctcaac tgtctatttt taatcctgaa
    11701 ttgttatttg agtaactcct ttttcctcat aaagttttct tcctctaaaa cttctggaaa
    11761 aaagcgaata gtttcagaca acatttttat aagaaacaag ttcatctgtc atttcaagaa
    11821 ggagtaatcc tttatctact aatggacgga acggaattca accgcttgtc cgatatgttt
    11881 tctaaggatt atatagcaaa atgaaataaa aacaggacaa atagatcggg acagtcaaat
    11941 cgatttctaa caatgtttta gaagtagatg cgtactattc tagtttcaat ctattatatc
    12001 tattatgcac accactatag gatctaataa aaatcacaac aagttcattc atagatggtt
    12061 acctcaggga actaagaaaa cgactacaaa ggaagtcgca ttcatcgaaa agtggattaa
    12121 caactatcct aaaaaatgct tgaactacaa gtcccccaga gaagacttct ggatgactaa
    12181 cttgaacttg aaatttagca ataattaatt caccatctaa ctatatttag taattatttc
    12241 agaactgatt aatattaaaa ttaactaaca attcaaagga ttcatactag ccataaatta
    12301 cgtccatcag agagagactc ttactacttt tagattttag tctttctagc ttcagaatac
    12361 atctaaactt taggaaaaat gactattcga aagcgcgaat gcctcaaaat tatctcagat
    12421 aagctattcg aaacttagaa tgcttttaaa tttatggaat tgcgattatt cgaaacctag
    12481 aatgcatata acctttagtt gacagaccta ttctaagtct cgaagggcta tttactttct
    12541 attccttatc aaaaaagact cattccccct ttctcctcca aaatatggta tagtagaaat
    12601 atactatcta tgaggagttt acatgtcaca ggataaacaa atgaaagctg tttctcccct
    12661 tctgcagcga gttatcaata tctcatcgat tgtcggtggg gttgggagtt tgattttctg
    12721 tatttgggct tatcaggctg ggattttaca atccaaggaa accctctctg cctttatcca
    12781 gcaggcaggc atctggggtc cacctctctt tatcttttta cagattttac agactgtcgt
    12841 ccctatcatt ccaggggcct tgacctcggt ggctggggtc tttatctacg ggcacatcat
    12901 cgggactatc tacaactata tcggcatcgt gattggctgt gccattatct tttatctagt
    12961 gcgcctatac ggagctgcct ttgtccagtc tgtcgtcagc aagcgcacct acgacaagta
    13021 catcggctgg ctagataagg gcaatcgttt tgaccgcttc tttattttta tgatgatttg
    13081 gcccattagc ccagctgact ttctctgtat gctggctgcc ctgaccaaga tgagtttcaa
    13141 gcgctacatg accatcatca ttctgaccaa accctttacc ctcgtggttt atacctacgg
    13201 tctgacctat attattgact ttttctggca aatgctttga cacgtaaaaa atccgtttgg
    13261 tttcccaagt ggatttttaa agcgtagatt aactatagct tgatactaaa tatactttgg
    13321 tatggaaatc atgcatattt ttcgatagtg aggcgaggac ttacctagcc tttccgccgt
    13381 gatagaaaca cctgaaatct aatggtttca ggtattcgga aactttgagc ctagtgtctc
    13441 aaagtttagg tatggaattt cgaagaaagt cgctaccgtc cgtaatcact taaggaaagg
    13501 ctcaaaaata ttgttttcaa ccacaaaatc cgtttggttt cccaagcgga ttttgtgctt
    13561 tattttgaaa cttcttttgc aagaacaaag ttcccaagtg tggcagaacc atttcctgcg
    13621 actgctggcg tcacgatata gtcacgcaca tctggtactg gtaggtaacc attaagaaga
    13681 gatgtaaatt tctcacggac acggtccagc atatgttgtt gagccatgac ccctccacca
    13741 aagacaatca cgtctgggcg gaaagtcact gtcgcattaa ccgcagcttg agcgatatag
    13801 taggcttgaa catcccaaac agggttgttg agttcaatag tttccccacg tacacctgta
    13861 cgagcttcca aacttggacc agctgcataa ccttctagac atcccttatg gaaaggacaa
    13921 acacccttaa actctttttc aatatccatt gggtgtctag caacataata atgacccatt
    13981 tcagggtgac ccacaccacc gataaactca ccacgttgga tgacgcctgc accgatacct
    14041 gtaccgattg tgtagtaaac caagttttcg atacgaccac cagcattgtt acgggcaacc
    14101 atttcaccgt aagcagagct gtttacgtct gttgtgaagt acattggcac gtttagggcg
    14161 cgacgaaggg caccaagcaa gtctacattt gcccagtttg gttttggagt cgtcgtgata
    14221 aagccataag tttttgagtt tttgtcaata tcaatcggcc caaatgaacc aactgcaaga
    14281 ccagcaaggt tatcgaattt tgagaagaac tcaatggttt tatcgattgt ttcgattgga
    14341 gttgttgttg gaaattgtgt tttttctaca acgttaaagt tttcatcacc gacagcacag
    14401 acaaactttg taccgcccgc ttccaagctt ccatataatt ttgtcatgat aaacctcttg
    14461 tttttatttt ctttattata gcatacttcg aaagtctaaa tgtctctatt ttttagattt
    14521 tcctctgtaa atcttactat ctaataaaaa cgaacaaaca tgtcatttgt tcgttttcac
    14581 attagagagg attgattaga ttttcacttc gatcacagca tcccccttcg aaactgaacc
    14641 tgttgcgact ggagctactg aagcgtagtc agctgtattt gtaacgataa ccattgttgt
    14701 atcatcaagt ccagctgcag cgattttgtt tgagtcaaat gttccaagaa catcgccagc
    14761 tttcacctta ttaccttgag caacttttgc ttcaaaaccg tcaccgttca tagatacagt
    14821 gtcaatacca acgtgaatca aaacttcagc accatttctt gttttcaaac caaaagcgtg
    14881 ccctgttgga aaggcaattg aaacttcagc atcagctagt gcatagacca cgccttgact
    14941 tggtttcaca gcgatacctt gtcccatagc tccacttgag aagactgggt cattgacatc
    15001 agcaagagcg acaacatcac cgacgatagg agttacaagt gtttcatttt gaagagctgc
    15061 tggcgcaact tcttcttttt cttcagccac ttcagcttgt tttgcagctg cagttgcgtc
    15121 tacttcatct tcgtaaccaa acatgtaagt aagagcaaaa ccaagggcaa atgatacagc
    15181 taccataaga aggtattgtg gaagttgtcc gttaccaaca taaagcattg taccagggat
    15241 gatggtgata ccattaccag taccagcaag tccaaggata gaagccaatc caccaccgat
    15301 tgcaccagca atcaatgaaa ggaagaatgg tttacggaag cgcaagttca ccccgaagat
    15361 agcaggctct gtaataccta ggaaggcaga aagagcagcc gggaaagcaa gtgttttcag
    15421 ttttggattt tttgttttaa caccaaccgc aacagtagca gcaccttgag ctgtcatagc
    15481 agctgtgatg atagcgttga atgggttagc atggtcagca gcaagtaatt gcacttcaag
    15541 caagttgaag atgtggtgca cacctgacac gacgatcaat tggtgaaccc caccaatcaa
    15601 gaaaccacca agaccaaatg gcatgctaag aatcgctttt gtagcaataa ggatgtagtt
    15661 ttcaacaacg tggaaaactg gtccaatgac aaagagtcca aggatagaca tgaccaaaag
    15721 tgtcacgaat ggtgttacca agaggtcaat gacatctgga acaaccttgc ggacagcttt
    15781 ttcaaattta gctccgacaa ccccgatgat gaaggctgga agaacggaac cttgcaaacc
    15841 aacaacaggg atgaaaccaa agaagttcat cgctgttact tcaccacctt gagcaactgc
    15901 ccaagcgttt ggaagtgagc cagagacaag catcatacca agaacgatac caacggcagg
    15961 atttccacca aatacacgga aggttgacca cacaaccaaa cctggcaaga tgatgaaggc
    16021 tgtatctgtc aagatttgtg tgtaagttgc aaagtcacct ggaagtggca tttcaagagc
    16081 gttgaaaaga ccacgcacac ccatgaagag acctgtcgct acgataactg ggatgattgg
    16141 aacgaaaaca tcaccgaaag tacggatagc acgttggaac cagttccctt gtttagcagc
    16201 ttctgctttc atgtcatcct tagatgatgt tggtaagcca agtacaacaa cttcatcgta
    16261 cattttgtta actgtacctg taccaaagat aatttggtat tgccctgagt taaagaaagc
    16321 accttgaact ttttccaagt tctcaatcac ttctttattg attttctctt catctttgac
    16381 catgacacgt agacgagtcg cacagtgggc aacactattg acattttcac gtccgcccaa
    16441 ggcatcgatg actttttttg caatttcctg attgttcatt tgcaaaaatc tccttatata
    16501 acattttgtt cttgtttgaa agcgatttta ttcgccctta cgactattat tttatcatgt
    16561 tttaaaaata tgtcaagcgt tttgcataaa aaatattcta tccacttaat gggcaagctt
    16621 tagattgatt gtttttgact gtttcatcag gaattccttg aaaaataagt ttgaaacctt
    16681 ggttaaaaag tcttttattt cattatcttt c
//
LOCUS       DAVKQL010000047        15475 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVKQL010000047 DAVKQL010000000
VERSION     DAVKQL010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15475
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.047"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(369..2249)
                     /locus_tag="VW561_001537"
     CDS             complement(369..2249)
                     /locus_tag="VW561_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU9943774.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIEGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDSLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDVL
                     CPILDRHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(2243..3112)
                     /locus_tag="VW561_001538"
     CDS             complement(2243..3112)
                     /locus_tag="VW561_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9943775.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILNKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGVSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAATGNALCQEAIERMNRNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDAYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(3196..5841)
                     /locus_tag="VW561_001539"
     CDS             complement(3196..5841)
                     /locus_tag="VW561_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEU9943776.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGEQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDDTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VAATGSLLSHSVLSIPQICPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTELIKKEEI"
     gene            complement(6032..7312)
                     /locus_tag="VW561_001540"
     CDS             complement(6032..7312)
                     /locus_tag="VW561_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEU9943777.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDEIF
                     VVATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCDVNDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            7483..9567
                     /locus_tag="VW561_001541"
     CDS             7483..9567
                     /locus_tag="VW561_001541"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEU9943778.1"
                     /translation="MKPLLETIDTRFGTTNKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGAWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGEDSLFHRQSSYNRDK
                     ASFQPHYLKIFSLRYQIETQLTPTCYGASIRLKQKQGKALSLYLHATDELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLVSSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKASAQADWENLLHRFDVIETGEANRTFFDHCLYRLFLFPQTF
                     YEVDESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            complement(9607..11286)
                     /locus_tag="VW561_001542"
     CDS             complement(9607..11286)
                     /locus_tag="VW561_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU9943779.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGACLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWANDAALPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGSGHTVGYKRLLRGAVVEAQKIRVVITESQVLPLLTKISLYKT
                     PRLSQTVAVQGLAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIQVLQFQAGESKKDLHLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            12041..13270
                     /gene="arcA"
                     /locus_tag="VW561_001543"
     CDS             12041..13270
                     /gene="arcA"
                     /locus_tag="VW561_001543"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEU9943780.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESEYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELILSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            13328..14344
                     /gene="argF"
                     /locus_tag="VW561_001544"
     CDS             13328..14344
                     /gene="argF"
                     /locus_tag="VW561_001544"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU9943781.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            14509..15456
                     /gene="arcC"
                     /locus_tag="VW561_001545"
     CDS             14509..15456
                     /gene="arcC"
                     /locus_tag="VW561_001545"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEU9943782.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVIVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
BASE COUNT         4358 a         3570 c         3183 g         4364 t
ORIGIN      
        1 catgagcatg atcatttgca aaatcgaaac tccataaaat cacatctgct agctccgcta
       61 acgtttttgt ttttggaatc tatatatgtc ggtagcaacc ttttttaatt tgacggaaat
      121 gatccaagcc tctacagatg ctggcttttc agcttctatt tcttgtttta ctaattttaa
      181 ctgtagcatt tgaaaccaga taattcttct gtttcacgcg aaacaccctg cgccaagcaa
      241 ggtgttcttg tttttatact caatgaaaat caaagagcaa actaggaagc tagctgcagg
      301 ctgctcaaaa cactgttttg aggttgtaga tggaagctga cgtggtttga agagattttc
      361 gaagagtatt aagtcgtata aatcgtcgac gctgtcgcaa tggtatgcca ctggttggct
      421 gttgtagctg cgaaatcctt gtctgcgtag aagtcagtaa atggtaggat ttcaacttcc
      481 agctcgtcga tgcggtcaag ctggccagcc agataaacct cgatacggct ttctgctcgt
      541 ttgatgcgtt gcaagagtcc gcccatacgg atgtcaactg tatccaaacc aaagaccttg
      601 ttttctttca gccattggtg gctaaagagg gcatggaagt cttcaatttg gcttctaagt
      661 tctggtaatt cttgtctggc gatttgttgt aaactttctt tatcatccgc ttggtaggcc
      721 tgacgaatgc gtcgtcccac atctactttg ctacttaaaa tagcattcaa ctgggcctga
      781 gtttcaaaga gataagcata gtttccagct ttttctttaa tgttagcaag cgtctcagca
      841 gcctgagcga agtgtggctt gtcctgttca ggtgtcatgt gtcggtcaag tatcggacag
      901 agaacatcct gataaaagac atatcggttg ggattgatgc cactgagatt acctggtagg
      961 tctggtaaaa ggttggcaag gtcaatctgc ataaaatctt caaccgttag accagtattg
     1021 gtcttgaaat gcgcagacaa actatctagg tcattgcgat agctgagttc tgcccagatt
     1081 tgcaagcttg gtaggataga gaactgggca gtttcaccac cattgtctcc ccaacccgtt
     1141 acgatgactt ctttaatctg attggcacgg caggctttat tagcctcgat agccactaga
     1201 cggctaaaat ggttgtgagg tgtaaagcca atccacttcc aagctccccc tgcaaatgca
     1261 aggtcatggc taatcttgtg atgattgcgg aaattacggt tgtatttttc ctcgctatcc
     1321 tgataataat cccagtaaac cagagtcaca cggtctttga gacggtctag gtagacacga
     1381 gtttcctctg gaatttccac atcacggtcg tactggccat ccgctgacat gagtttgaag
     1441 aacatatcac tccacatctg gcagtggaaa ccatatttgt cagcaatatc cagcacgcgc
     1501 tccaagtgtt ggcacatgag gagactacga tccacaacac cgttcaaaat caggtagcgt
     1561 cccaaaccaa ccaagtgggc ttcgtccatc ccgatattga ccttgcgagt cttcagttta
     1621 gacaacgtgg caaacatgcc atcaatcagg tcataaacct tttcttcgcc aatgagaaga
     1681 atgtcctcta catcacggag ctcctgcact tccttgacac cccatttgac aaaggccgac
     1741 aagtgggcca aggtctggat gcatggtaca aaggtcatgt caaactgttg agcataggct
     1801 tcgatttcct gcaactcctc tgctgaataa gctccacgga aatagccaaa gtaaggctgc
     1861 ccttcaatct ggtaagtgtc ttccatgtaa agttcaaagg ttgagtagcc catgagagcc
     1921 aagacctcaa tcatctgctt ggcagaagcc acattcagca ccgcatttcg agaacagtca
     1981 accatgtaag ccaaatcttc gtaagctgct tgttcctcaa tctctacttt atcagcttct
     2041 gctagaactg ttaccaacaa ggataaggca cgataaagtt ggtgaggttt gcggtaggtc
     2101 aattgatagt gaccttcctc accctcgata gagatagagg cttggtcaga ctgagtgaca
     2161 gccacttcca catctggtag agaaatgtga ccttttaaaa cctcaatagc ttgcgtttgt
     2221 ttgggactaa gtcctgtaaa tcttaccatt gcttttcctc ctgtagccag ttgacaaggg
     2281 caccgtagag atttgcatct gcgtgatagg tgcaggcctg gataactggt gcgaccgtgt
     2341 attcttcgta ggcatctaca aagtcttcaa cagccttctt aacaccttgg ataaaatctg
     2401 gattttgact gatagagcca cccagactga tgacatctgg atcgatcaga tactgaatat
     2461 tgagcaagcc ttgcgccaga ttgcggttca tgcgctcaat ggcttcttga caaagagcat
     2521 taccagttgc ggcctcttgg taaatcttgc gaccgtccca atcagtatga ccagattttt
     2581 caataacgta tcgtaccata ttcccagttg atgctagttg cgaccagtta ttaagttttt
     2641 cagcaggggc aagggttgtc atgtaaccaa attctccacc cagaccgtgg cgacctcgat
     2701 gaagtctacc attgataatc atggctccgc caatccctgt cccaatcacg acacaggctg
     2761 cattttcaag ctctggatga gctagtagtt cactgactcc aacgcagttg gcatcatttt
     2821 ctaaatggac aggtagctga taagagctaa gcgcctcata ccaagaaaag ccatggatgt
     2881 agggcaccgc actgaagcca tcaatcacac ctgtctcttg attgaccgca cctggaacgc
     2941 tcatagcaat cccactgtaa tcctgttctg acaagcgttg atctagccac gctagtaaat
     3001 cctccaagtt ttcaggcgtt gaaatacttg tcttattcag tatttttcca tcaggagtca
     3061 gactggcaaa cttaattcca gtccctccga tatcaatcgt tgcaatggtc attgttttta
     3121 ccataaaaag gggcgaatca gcagttagtt cttactcttt cgcccctcct ttctctttat
     3181 gtttactttt tgaaattaga tttcttcttt tttaatcaat tctgtacgaa tttcttgtgg
     3241 agccaatagt cctgaatgaa ctgggtatgg tcgttcaagt aggtcaagaa tagtttgttg
     3301 gtgttctgat atgcgcacat tttcttgact catattgtag taacggagta catatccttc
     3361 ttcattttcc gccactttaa aggctgttgg acaaatttgc ggtatgctga gaacagaatg
     3421 gctcaagagg ctaccagtcg cagccacgct tccttcctgt ctagcaagct gaaggctggt
     3481 aaacggtgtc tgcaaggcct tagctcgacg ataagctgag aagcgttctt gggcttggtg
     3541 gcactcaagc gcaaactcga cttcaaactc ccgcaagcat tgtgcttctg gtgttgggaa
     3601 gtagccccag tcacctagct cacctgatgc acgcaaaata gtcactgcaa tggtgtcgtc
     3661 tccaaggatt tcgtattcat tcaatccctt gttggataca gtcacacctt tttcatcgtc
     3721 atacagacta acaaaggctt gttggtgttg aggattttca ggattttccc atgaagcagc
     3781 tggtttgttt ggtcgtgtca ccacctcata gatactttca gaatcattgc ttggacgcgt
     3841 gttatgagtc ttgaccaaga gacggatacg gtgatccttg gcagtgttag taaagcgagt
     3901 cttgaagcgg atttgtggat tgtcaacgaa gacagtcaac tcagtttcca gaggaatgtt
     3961 tgtcaattct tctgaccgtc cagcctcacg cttcataaac tcgatgatac cttgttgctc
     4021 ttcttctagc ttttcatccg cactgacagg cacggtcaat tcatgtttga gcaagatttt
     4081 agcatagcga tctgtgtttt ccaagacctc gtggccctta agctctgcaa agattggctc
     4141 tgttcctttt ggttggaaat agatatactc gtttccgatg tcaccccggt cttcgaagcg
     4201 gataaaatct tcataggctt cgtgagttgt cttatcatag actgtgatgt tgtcatccac
     4261 actcaccgtt acgaatggtg tatcaatcac tccgttttgg taaataccct cacggtgttc
     4321 ttgttctcct tccagcaatt ggaaggttgt ccaagaaagc ggtgctaggt gaactggaat
     4381 ggtcacgcgc acttgtcgag caatacgagc ttggcggaac ttatcttttg gtaaatcgta
     4441 ttcaaaatta gctccgaggt cttcgattgt agcctctaca ggacgaccat ccaagtcctc
     4501 cacacggtaa cttggcaaga taagagcagc catctttttg tagccttctg ttgggtgcaa
     4561 ttccttgaaa tcacaagtcg ccacatcaat cactgtgctg acagtatcga ccttatcatg
     4621 caagcctgtg ttaatgacag taaagagata gtcactttga gccttatccg tagcaatttt
     4681 acccttccac tcgttgagca aattactttt aacaaagttt cctacttggt tgaccttggc
     4741 aaaacgcgtt tccatctcgc ggtgaacttc gtccacgcta cagccacaga tactatcatg
     4801 tggcgcattc tgcaaaagtg ttttccaagc ataggtcaac tggtccttgt ggttgtgtcc
     4861 accagtgata atagtcaagg gttctaccac ttgctctagg aggttgctat tttcttggaa
     4921 ggcttgtttt aggtaaatac gggatgaaga agtgttggca agtgtgtacc agccatctgt
     4981 ttcctgactg gtcaactcgc ctgtaacagt tgataagtgt tcaggaagcg caccttctac
     5041 agcttgaaca tattcatcaa aagaactatg aacaaagatt acatccggga agagttcatt
     5101 tgccacacga atggcttcgc tcagattttt ctgtacaggc tggtggtcac agccgttcat
     5161 catcaaccat tggttggtcg aagcgtaggc acgcacatct gacaattttt gtttccagaa
     5221 ggtcaaggcc tcatctttgt caactgggat ttcattccca ttactgtacc agttggcaaa
     5281 gagaatacct aaaacacgac taccatccac accctgccag tacatttctg aaaactggga
     5341 tgtaaactgc tcatcttcaa ggacttggtt gtcaaatcca atcggcttca caccacgacc
     5401 aaaggccgcc acgtgaatgc ctgatttttg aagaatttga ggcgcttgtc ccatatttcc
     5461 aaaggtatct ggaaagtagc caatctgggt tgatttaccc catttggcag cttcttgttg
     5521 accaatcaag gtattgcgga cattggcttc actggagatc aaataatcat cctgcaagat
     5581 gtaaaaggga ccaattttaa gcttgccctc gtcaatgtag cgttggacct tatcacgatt
     5641 ttcagggcga atttgtaagt agtcgtcaag gacaatagtt tgtccatcca agtggaaact
     5701 cttgaactca gggtcatttt caaagagatc aaagagattg tcaaacaatt ccaccaactg
     5761 catacgatgg ctttcaaaag gcaagtacca ctcacggtcc cagtgactat gtgagataat
     5821 atgtacaaca acattttcca tgaagtaaaa cctcattcta aatttaaatt ttaacgtttt
     5881 aaatgtaaac ttgtgattct gataagaatc ggttgagcta atactcaacg aaaatcaaag
     5941 tgcaaactag gaagctagcc gcaggttgct caaagcactg ctttgaggtt gtagataaag
     6001 ctgacgttgt ttgagagatt ttcgaagagt attagcgaat atccaagtaa tccaagacca
     6061 actcacagaa catcatgtta gcccaggaga accattcacg agagtagagg gtcggatcat
     6121 ctacgtggaa gctttcgtgc atgacacctg taccaccatc gcaggcaacc agctgatcca
     6181 gcaagaattt tttctctgcc ttatctcttg ttgtcaatcc ttggatagaa agggcgattg
     6241 gccagatata gcgatagaag gtatgagaac ttccgagacc gctagcgtat tctccttggt
     6301 agaagtatgg attttcagag ctcaaaatgg tacgacgagt agcttgatac acttcatcgt
     6361 tgacatcgca gtagcccaga tagggcgcag ctagtagact tggtacattt ggatcatcca
     6421 tgatgctggc atttcctagg ccatccactt caaaagcgta aatcttttcg cccttgctgt
     6481 tggtggtgta agcgtagttt tcgattcctt cttggatttc atcctgaaga cgcttggcat
     6541 cagcaataac actctggcta tcagctaggt ttaatgctgc gaagatttct tgcacataac
     6601 ccaagactac tacagcaaac atatttgacg gaatcaagta actatactgg caacagtcat
     6661 cactcggacg aaaggctgac caagtcatac ctgtcactgc aaagtcaggt ccaaagccat
     6721 catttaccaa ggtgtcttcc ttacggtccg tatctcggac aaaacgataa ggagagttct
     6781 tgtggtcttg ttcaactgtc catagatgga gaatttcctt agtcgctacg acaaaaatct
     6841 catcaaactg actagtctcg ccagtctctt tccagaggag ataagccaac tgcaaaggat
     6901 agcaaagtga atccacctca tacttgcgct cccagatcca gccgttaagg tctgtgtgat
     6961 cagtctcgtg gtgccctttc cagttctcct caatgttgaa ggagttagca tagggatcct
     7021 tgagtaccaa ggtcatctga cgtttgacca aacctgcaat ggtctgacgc aggagggcat
     7081 ctcttttagc tacatgaagg tagggtctga gttgggctgt cgaatcccga agccacatgg
     7141 caggaatatc cccagtcaag acaaaagttg aaccatcttc taagatttca accgtattgt
     7201 ccaaggtatc ggtgtagcaa cgctcgaaaa catccaccca ctctggatgg tccttagccc
     7261 gctctgctac ttcatctagc cattctctaa caatttcttt cgaataaatc atcgtgcaat
     7321 tccctctttc aaacaagttt catgttcagt atatagcttt tgagacagaa aatacataca
     7381 caaatgatag tcatttttct acaaaatagt tatttttgaa actccttttc taaaggccct
     7441 ctttttctga tataatgtag aaaaacagct aaaggaaaga ctatgaaacc actacttgaa
     7501 accatcgata cccgctttgg aactaccaac aagcatgcct tttctcgggg aaataccctg
     7561 ccatacacag gcgtgccttt tgggatgaat tattttgtgc cccagaccag tgaccaggac
     7621 ggagcttggt tcttcgatcc gcatctgcct atctttcagg ggattcgatt aactcaccag
     7681 cccagtcctt ggattgggga ctactcttgg ctccttctga cacctgtcac cagtcaactg
     7741 ggtgaagaca gtctctttca ccgccagtct tcctataata gggataaggc ctctttccaa
     7801 cctcattatc taaagatttt ctccctgcgc tatcagattg aaacccagct cacaccgact
     7861 tgctacggtg cttctattcg tttgaagcaa aagcaaggca aagccctctc cctctatctt
     7921 cacgcaacag atgaactgac agtagagcaa gtagataagc gaactcttgc cctgcgacaa
     7981 gaaggtaaaa ctgaaaccaa caaaaattcg ctaacgatgt tcactgccct acaaatgaac
     8041 acggatattc ttgctatcag ccaagaagct ggagactggc gaattgactt agtaagtagt
     8101 caaaccgaga tgcaactagc cacttctttc atctctcctt ctcaagctct aatcaatcta
     8161 cctcaagaag attttgatag ctgtaaagca agtgcccaag cggattggga aaatctcctc
     8221 catcgttttg atgttataga gacaggagag gctaaccgaa ccttctttga ccactgtctc
     8281 tacagactct tcctcttccc acagactttt tatgaggttg atgagtcagg gcaagccatc
     8341 cacatggatc tggctactgg tactgtcaag cctggtgtcc tctttagcaa caatggtttc
     8401 tgggatacct tccgcaccac cttccccctc tttgccctta tcataccgga gcactatcaa
     8461 cgctttttag aaggtttcct caatagctac cgcgatactg gtttccttcc aaaatggctg
     8521 gctccagatg aacggggtat gatgccaggt acacttttag acggtattat cgcagatagc
     8581 gcctgcaagg acatggcccc tgacctagaa ggagaactct tccaagccat gctcgaaaca
     8641 gccagcaaag ccgaccctct cggcatcaat ggccgccacg gactagccca ataccaagaa
     8701 ctaggttacc tctctaccga ccaccacgaa agtgttagcc atactctaga ctatgcctat
     8761 agtgactttt gtatcgctag ctgtgccaaa aaactagaga acatagaaat cgctgaaacc
     8821 tacaaggctg cttcacaaaa ttaccgccag ctatttgacg ctgagacagg ttacatgcga
     8881 gcacgagaca atcaaggaaa ctttcaccct gacttctctc cttatagttg ggggcgagac
     8941 tacgctgaat gctctgccat tcaagctact ttaggcgttc tccacgacat ccctggctta
     9001 atccaactga tgggtggaaa agaaaccttt agcaactatc ttttgaaagc ttgtcaagat
     9061 gctcccctct ttgaaacaac aggctatggt tacgaaattc acgaaatgag cgagatggct
     9121 actgctcctt ttggacaaat agccatttcc aaccagccta gtttccacat tccttatctc
     9181 ttccgctaca gcgattaccc tgactacact gcccttctta tcaagacgct ccgtcagaaa
     9241 gcttttcacc caagttggga agcctatcct ggagatgaag acaatggtag tctctctgct
     9301 tggtacatct ggtcagctct cggattttat ccaacctgtc caggaaaacc aagctatgac
     9361 ctcggaatcc ctctctttga ccatctacga gtctacctcg ctaaagaaga taaatggctg
     9421 gatatccata ctaaacaaaa ccacaaccat tttaactttg tcaaagaatg ccgactggac
     9481 aaaacactcg tatcaactat tcaacaccaa gacctcttaa aagctgaaca actaactttc
     9541 accctcagct ggttaccaag tcactaataa ccaaaagaac ctttgcatcg cgcaaaggtt
     9601 cttcttttat gaaacttgga cttgaagttg atccacaagc tgaccatcca ccgttaggtt
     9661 cagatagaaa tccaaggttt tatctcctgc gaaatacagg gttggtagat gcaaatcttt
     9721 tttgctttcc cctgcttgga attgaaggac ttgaatctca tcctgatagg cgacaccatg
     9781 gacacctgtc cccggttgaa tcgaaatctt agcctctaaa gaggagctac tttcactacg
     9841 ttcaatccta aaatgaagag tctctccctt tgccacagct aggctttttt ctgcaaatgc
     9901 tagcccctga acagctactg tttgtgataa cctcggagtt ttatagagtg aaatcttggt
     9961 caacaaaggc aaaacctgtg attctgtaat gactacacgt atcttctgtg cttcaacaac
    10021 tgctcctcgt aagagacgtt tgtaaccaac agtatgaccc gaaccaaact cctgccagac
    10081 accatccacc tctacttgca catgaaaagc agcgattcgt tgccctagct tcaaatcttc
    10141 tcttaactca attacatcaa aagttttagg agaacctaag tcgagttcta actggatggg
    10201 caaggctgca tcgtttgccc aagagctggt ctcaaggccg tctgtcaaat ggcgacaagc
    10261 aaagtctgcg gaaagagctg gaccagatac ctcagctccc agagccaaat cttctttata
    10321 gagctcattg cgataggtcg caaattcata aagtcgttca atatcctttg catcaaagag
    10381 cccagcttga ttcggcggaa tattaagcaa gagtggagtt cctcgcccta ctgagtgaaa
    10441 gtagatttcg accaactcct cgagagactt aggatcctga tcctcatggt agaaccagcc
    10501 tggacggatg gaaacatctg cctctccgat tgaaaaaatc gtgcccgagg gatccccgtg
    10561 ctgaagatag ttcagctctg cttctgttcc tagtttatca ggattcacct tttgccacag
    10621 tggatcacct gcataccctc gttcattgcc aatccagcgg atactggtgc cttctgttga
    10681 aaaaatcaag caagcgccct gcaggtcacg aatggtttca aaccattttt caaattcata
    10741 attaaccttt tgcgcgccct ctcctctggc accatccatc caaacctcag cgaacttacc
    10801 agcattccca tagttaggat ttgataagat ttccttcaac tgagccagat aataggcatt
    10861 gtagtccgct tctcggtcca catgatagag gggactatgg gcatcccacg gtgacaggta
    10921 gacccccata tccatatcaa actctgtggc agcttgggat acttcaagga gcaagtcgcc
    10981 ctttcctctc ctccaaggac tgaccttaac cgaataatct gtgtgagctg tcggataaag
    11041 gacaaagcca tcgtggtgct tgaccaccaa aatcaacttt ttgaagcccg tttccttgag
    11101 cacacgaacc cactcacgcg catccaactg actcgggtta aagcgctcag gatcctcctg
    11161 tccagtcccc cattcttggt cataaaaggt attaggaccg aagtggataa aagctgctag
    11221 ttcatctccc agataagcta gctgagtctg acttggtaac ggtccatgcg gtttaatttt
    11281 cttcatcgtt atatatctcc ttctttagca acttctcttg ttcacaaggt tatccacagg
    11341 ttgtggataa taaaccatga tctaaccgcc agctctttcc aactccaact ggtaggctca
    11401 ggatttttct cctcaatatt ccctcttcaa acagctctgt catatggttc tgctaacacc
    11461 tctcagaatg ctactctcca taattattct caggcttctt tcaggtcatt gtgtgaagac
    11521 cattcactct ccagactttt ccacagattg tgaataagtg attattctaa actatcacct
    11581 cgtcgactat aacaaaaaat aaaaagaggg tcaataagat aattatctct tcctaaatac
    11641 tcagaaaaga ttctataatt tgtacaattg aacaagaaat gcttctccaa aatctatctt
    11701 caaaagaatg ttgcatcttt ttatcctttc acacaaacat ttctctttat acagatccaa
    11761 gtgataacca gatgataatt ggacactttt tggtcatttg tatatttttt gaaaaatttt
    11821 ttaaaaaaat tttatatcct ttttatagaa gtttatgcat ttgtatctat gtattctatc
    11881 ctatgttatg tataataaca taaaattgtg ctaaacatca taatttatat tattttccga
    11941 gaaattatag ccattgacat tacctaggaa ttgcgttata acatacatgt aagcggtacc
    12001 caaaatggtg ccaagtcaaa atttttaagg aggaaaatac atgtcttctc atccaattca
    12061 ggtcttctca gaaattggga aactgaaaaa agttatgttg caccgtccag gcaaggagtt
    12121 agaaaacttg ttgccggact atcttgaaag gcttcttttt gatgatattc ctttcttgga
    12181 agatgctcaa aaagaacatg atgcatttgc ccaagctctt cgcgatgaag gaattgaggt
    12241 tctctaccta gaacaactcg ctgctgaatc attgacctct ccagaaatcc gcgatcaatt
    12301 tatcgaggaa tacttagacg aagccaacat ccgtgatcgt caaaccaagg ttgctattcg
    12361 tgaattgctt cacggcatca aggacaacca agaattggtt gaaaaaacaa tggctgggat
    12421 tcaaaaagtt gaattgccag aaattcctga cgaagctaaa gatctaactg acttagttga
    12481 atcagagtat ccatttgcaa ttgacccgat gccaaacctc tatttcactc gcgacccatt
    12541 tgcaacaatt ggaaacgccg tatcgcttaa ccacatgttt gcagatactc gtaaccgtga
    12601 aacactctac ggtaagtata tcttcaaata ccacccaatc tatggcggaa aagtggattt
    12661 ggtctacaac cgtgaagaag atacgcgtat cgaaggtgga gacgagctaa ttctttctaa
    12721 agacgtcctt gcagtaggta tctctcaacg tacagacgca gcttctatcg aaaaactttt
    12781 ggtcaacatc ttcaagaaaa atgttggctt caagaaagtt ttggcctttg aatttgctaa
    12841 caaccgtaaa ttcatgcact tggatactgt cttcactatg gtagactatg acaagttcac
    12901 tattcaccca gaaatcgaag gcgaccttca cgtttactca gttacttacg aaaacgaaaa
    12961 acttaaaatc gttgaagaga aaggtgactt agctgaactt cttgctcaaa accttggtgt
    13021 agaaaaagtt catttgattc gttgcggtgg tggcaatatc gtagcagctg cgcgtgaaca
    13081 atggaacgac ggttctaaca ctttgaccat cgcacctggt gtggtagttg tttatgaccg
    13141 caataccgtg accaataaga ttttggaaga atacgggctt cgcttgatta agattcgcgg
    13201 aagtgaattg gttcggggcc gtggtggacc tcgttgtatg tctatgccat ttgaacgtga
    13261 agaagtgtaa tcgctgttcg gcattcgtca atagaaaatg taaaaaatag aaagaggaaa
    13321 taataaaatg acaaattcag tattccaagg acgcagcttc ttagcagaaa aagactttac
    13381 ccgtgcagag ttagaatacc ttattggtct ttcagctcac ttgaaagatt tgaaaaaacg
    13441 caatattcaa caccactacc ttgctggcaa gaatatcgct ctcctatttg aaaaaacatc
    13501 tactcgtact cgtgcagcct ttacaactgc ggctatcgac cttggtgctc acccagaata
    13561 cctcggagca aatgatattc agttgggtaa aaaagaatct actgaagata ctgctaaagt
    13621 attgggacgt atgtttgacg ggattgaatt ccgcggattc agccaacgta tggttgaaga
    13681 attggcagaa ttctcaggcg ttccagtatg gaacggtcta actgacgaat ggcacccaac
    13741 tcaaatgctc gctgactact tgactgttca agaaaacttc ggtcgcttgg aaggcttgac
    13801 attggtatac tgtggtgatg gacgtaacaa cgttgccaac agcttgctcg taacaggtgc
    13861 tatccttggt gtcaatgttc acatcttctc accaaaagaa ctcttcccag aaaaagaaat
    13921 cgttgaattg gcagaaggat ttgctaaaga aagtggcgca catgttctca tcactgaaga
    13981 tgctgatgaa gcagttaaag atgcagacgt tctttacaca gacgtttggg tatcaatggg
    14041 tgaagaagac aaattcgcag aacgtgtagc tcttcttaaa ccttaccaag tcaatatgga
    14101 cttagttaaa aaagcaggca atgaaaactt gatcttccta cactgcttgc cagcattcca
    14161 cgatactcac actgtttatg gtaaagacgt tgctgaaaaa tttggtgtag aagaaatgga
    14221 agtaacagac gaagtcttcc gcagcaagta cgctcgccac ttcgatcaag cagaaaaccg
    14281 tatgcacact atcaaagctg ttatggctgc gacacttggt aacctttata ttcctaaagt
    14341 ataattttag ataataaacc gtctaccaac agctatgagg gctgcgacta atagctttag
    14401 tccggtcctc ttttatataa tggtaatcta ttatttctta taaaatatgt gaaaaatcat
    14461 taaattgaaa tctaaacgca ttctattgag tgtgataaag gagaatttat ggcaaatcgt
    14521 aaaattgtag tagctttggg aggaaatgcg attctttctt ctgacccatc agcaaaggct
    14581 caacaagaag ctttagttga aacagctaag catcttgtaa aattgattaa aaatggagat
    14641 gatttgatta tcactcacgg taatggacct caagttggga atctcttgct ccaacatttg
    14701 gcatcagact ctgaaaagaa ccctgccttc ccactcgact cacttgtcgc tatgacagaa
    14761 ggtagcatcg gtttctggtt gaaaaatgct ttgcaaaatg ctctcttgga tgaaggcatc
    14821 gaaaaaaatg ttgcctctgt tgtaacgcaa gttatcgtag ataaaaatga tccagctttt
    14881 gttaacttga gtaaaccaat cggtcctttc tattcagaag aagaagcaaa agcagaagcc
    14941 gaaaagagcg gagcgacttt caaggaagat gctggccgtg gctggcgtaa ggtcgttgcc
    15001 tcaccaaaac ctgttgacat caaagaaatt gaaaccatcc gtactctttt aaataatggt
    15061 caagtcgtcg tagctgcagg tggtggcggt attcccgtcg tcaaagaaaa caatggacat
    15121 ttgactggtg tcgaagcggt tattgataaa gacttcgctt cccaacgttt ggcagaattg
    15181 gttgatgcag acctcttcat cgttttgaca ggtgtagatt atgtatttgt taactacaac
    15241 aagccaaacc aggaaaaatt ggaacatgtg aatgttgccc agctggaaga atatatcaaa
    15301 caagatcagt ttgcaccagg tagcatgctt ccaaaagtag aagcagctat cgcttttgtc
    15361 aatggtcgtc cagaaggaaa agcagttatt acttcccttg aaaatctagg cgccttgatt
    15421 gaatctgaaa gcggaacaat tattgaaaaa ggataagttg ttttactaat aagat
//
LOCUS       DAVKQL010000048        15218 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVKQL010000048 DAVKQL010000000
VERSION     DAVKQL010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15218
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.048"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            <1..697
                     /locus_tag="VW561_001546"
     CDS             <1..697
                     /locus_tag="VW561_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEU9943783.1"
                     /translation="HQDSGNTEAKGAEAIYNRVKAAKKVPLDRMPYNLQYTVEVKNGS
                     LIIPHYDHYHNIKFEWFDEGLYEAPKGYTLEDLLATVKYYVEHPNERPHSDNGFGNAS
                     DHVQRNKNGQADTNQTEKPQTEKPEEETPREEKPQSEKPESPKPTEEPEEESPEESPE
                     ESEEPQVETEKVKEKLREAEDLLGKIQDPIIKSNAKETLTGLKNNLLFGTQDNNTIMA
                     EAEKLLALLKENK"
     gene            1116..1496
                     /locus_tag="VW561_001547"
     CDS             1116..1496
                     /locus_tag="VW561_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEU9943784.1"
                     /translation="MESIGIGLVIVSHSKHIAEGVVELISKVAKDVPITYVRGTEGGG
                     IGTSFEQVDRVVSENPADTLLAFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNA
                     AALLQAGAELSVIQTQLAELEINK"
     gene            1954..2667
                     /locus_tag="VW561_001548"
     CDS             1954..2667
                     /locus_tag="VW561_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEU9943785.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFAHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYNLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            2725..3327
                     /locus_tag="VW561_001549"
     CDS             2725..3327
                     /locus_tag="VW561_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943786.1"
                     /translation="MSEVDFNEAVNYEFTSDTCQLANSIYQSLFKFFDKKNFSGDLIF
                     TWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLNMNRNGCIGDFPHDFF
                     DTYLDHVAKYAYEQKVNNIKEYYPLKRAILHQENALYFRLFSNFDDFLEKNYLKTIWQ
                     VSKETPFSEMDFNMFKNISEKIIFERGSKMLNDLKSNYKK"
     gene            3465..4118
                     /locus_tag="VW561_001550"
     CDS             3465..4118
                     /locus_tag="VW561_001550"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU9943787.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGTIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            4128..4592
                     /locus_tag="VW561_001551"
     CDS             4128..4592
                     /locus_tag="VW561_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9943788.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAVREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGM
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            4605..5873
                     /locus_tag="VW561_001552"
     CDS             4605..5873
                     /locus_tag="VW561_001552"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU9943789.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGHVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLG
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFAAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            5887..6147
                     /locus_tag="VW561_001553"
     CDS             5887..6147
                     /locus_tag="VW561_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943790.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            6158..7519
                     /gene="pdrM"
                     /locus_tag="VW561_001554"
     CDS             6158..7519
                     /gene="pdrM"
                     /locus_tag="VW561_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEU9943791.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFAFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            7673..8641
                     /locus_tag="VW561_001555"
     CDS             7673..8641
                     /locus_tag="VW561_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU9943792.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            8657..9649
                     /locus_tag="VW561_001556"
     CDS             8657..9649
                     /locus_tag="VW561_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU9943793.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVILVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            9867..10910
                     /locus_tag="VW561_001557"
     CDS             9867..10910
                     /locus_tag="VW561_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEU9943794.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            10974..12659
                     /gene="lpdA"
                     /locus_tag="VW561_001558"
     CDS             10974..12659
                     /gene="lpdA"
                     /locus_tag="VW561_001558"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU9943795.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHSAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVAVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            12723..13712
                     /locus_tag="VW561_001559"
     CDS             12723..13712
                     /locus_tag="VW561_001559"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEU9943796.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEAVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(13785..14855)
                     /gene="xerS"
                     /locus_tag="VW561_001560"
     CDS             complement(13785..14855)
                     /gene="xerS"
                     /locus_tag="VW561_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEU9943797.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLVIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVSDEQKNALDSL"
BASE COUNT         4676 a         2756 c         3298 g         4488 t
ORIGIN      
        1 ccatcaggat tcaggaaata ctgaggcaaa aggagcagaa gctatctaca accgcgtgaa
       61 agcagctaag aaggtgccac ttgatcgtat gccttacaat cttcaatata ctgtagaagt
      121 caaaaacggt agtttaatca tacctcatta tgaccattac cataacatca aatttgagtg
      181 gtttgacgaa ggcctttatg aggcacctaa ggggtatact cttgaggatc ttttggcgac
      241 tgtcaagtac tatgtcgaac atccaaacga acgtccgcat tcagataatg gttttggtaa
      301 cgctagcgac catgttcaaa gaaacaaaaa tggtcaagct gataccaatc aaacggagaa
      361 acctcagaca gaaaaacctg aggaagaaac ccctcgagaa gagaaaccgc aaagcgagaa
      421 accagagtct ccaaaaccaa cagaggaacc agaagaagaa tcaccagaag aatcaccaga
      481 ggaatcagaa gaacctcagg tcgagactga aaaggttaaa gaaaaactga gagaggctga
      541 agatttactt ggaaaaatcc aggatccaat tatcaagtcc aatgccaaag agactctcac
      601 aggattaaaa aataatttac tatttggcac ccaggacaac aatactatta tggcagaagc
      661 tgaaaaacta ttggctttat taaaggagaa taagtaaagg tagcagcatt ttctaactcc
      721 taaaaacagg ataggagaac gggaaaacga aaaatgagag cagaatgtga gttctagttc
      781 tcattttttt atgaaaatgt gcaaaatata gtagattgaa attagaatag tatacctcta
      841 cttctaaaac attgttagaa atcgatttga ctgtcctgtt cttatttcat tttactatat
      901 cttaacagat agtgtaaata aagataaact atttactggc taattaatca gttaaacact
      961 agttaaggag taatgataaa aaagaacaat actattattg atggccagtc tgttggctct
     1021 tgtcttagga gcatgtggtt ttttggacat attgatcctg gatcattctc atcaggatta
     1081 ctctttactg ctattttaga aactggggtg gtttgatgga aagtattggt attggtcttg
     1141 ttatcgtttc acattccaaa cacattgcag aaggtgttgt tgaactgatt agtaaagtag
     1201 ctaaagatgt tccgattact tatgtaagag gaaccgaggg cggaggaatt ggaacgagtt
     1261 ttgaacaagt agatagggtt gtttccgaaa atccagcaga tactttactt gccttttttg
     1321 acctaggttc tgctaaaatg aacttaaaaa tggtgactga tttcagtgat aaaagtatca
     1381 tcatcaacag ggttccaatt gtagaaggtg cctataatgc agctgctctt cttcaggctg
     1441 gtgcagaact gtcagttatt caaacacagt tagcggagct tgaaatcaat aaataaggaa
     1501 ttttactata actcttttta taggtaagct attgattatc tcaactataa aatgttaagt
     1561 aaatgatttc aaaatcagaa agggattgct ttatgcagtt cctttttatt ttaacaggag
     1621 tgaaactata gtgtttctaa attgtgaatc aatcaaaact gattgtgatg gggctattct
     1681 agctttagaa accttcaaaa attaaaattt aaggcaatca attacttgga agagtatgaa
     1741 agcatttagt ttataggaat tctaggtcta gaattacata tatatttatg aagacggggt
     1801 gttcgatagt tagtattgtt ctattctgaa agatttgagc tgtcagttgt atagaaagtg
     1861 ttcgaatttt tttaagtgat taaattagtt aattgtatga ggtgctttat gatataatgt
     1921 tcttaatgaa ttttcagaaa ggaaaacctc aaattgttct acaaatttct actcttcgac
     1981 ctcgaccaca ctcttcttga ttttgatgct gctgaggatg tggctttgac ccaacttcta
     2041 aaagaagaag gagttgcgga tattcaggct tataaagatt attacgttcc tatgaacaag
     2101 gctctctgga aagacttgga gctgaagaaa atcagtaaac aagagctggt taacacgcgc
     2161 ttttctcgtt tatttgctca ttttggacag gaaaaagacg gtagttttct tgcccagcgt
     2221 taccaatttt acctcgccca gcagggacaa acactatcgg gcgctcatga tctcttggac
     2281 agcctcattg agcgtgatta taacttgtat gctgcgacaa atggcattac tgccattcag
     2341 acaggacgtt tggctcaatc tggtctagca ccttatttca atcaagtctt tatctcagaa
     2401 cagttgcaaa ctcaaaagcc ggatgctctt ttttatgaaa agattggcca gcaaattgct
     2461 ggatttagta aagaaaagac gctgatgatt ggagattctc taaccgccga cattcaaggt
     2521 ggcaataatg cggggattga cactatctgg tataatcctc atcacctcga aaatcacaca
     2581 caagcccagc cgacttacga agtctattct taccaagact tgctggattg tttagataaa
     2641 aatattcttg aaaagatcac attttaaagg agacgagcta atgactacaa aaaagctaat
     2701 attactattg aagagtacat tgaaatgtct gaagttgatt ttaatgaagc tgttaattat
     2761 gaatttacat ctgacacttg tcaattagca aatagtattt atcaatctct ttttaagttt
     2821 tttgataaga aaaatttctc tggcgattta atttttactt ggaaatctcc atcattagtc
     2881 aaagaagggg attatattgg gagaagggat tcacaagtag ataatcttag agtaatagga
     2941 aatatatttc cgaattatct tactaatcga aaatatagcc tcaatatgaa tcgtaatggc
     3001 tgtataggag attttcctca tgactttttt gatacatacc tagatcatgt agcaaaatat
     3061 gcctacgaac aaaaagttaa taatattaaa gagtattatc ctttaaaaag agcgatttta
     3121 caccaagaga atgcattgta ttttcgattg ttttctaatt ttgacgactt tttagaaaaa
     3181 aattatttaa agactatatg gcaagtttct aaagaaactc ctttttctga aatggatttt
     3241 aatatgttta aaaatatttc agaaaagata atcttcgaaa gaggtagtaa aatgcttaat
     3301 gatttaaaaa gtaattataa aaaatagcca gtctttataa actgtattca cgtattcaaa
     3361 tcgtatatat agtcgattta ttaaatcttt agataaactg taaaaagtgg tttccatagc
     3421 cactttttgc tataatagag gcagtaaaaa tgaaggagtc agctatggaa cactcgtctt
     3481 ggcacgcttt gattaaggcg caattacctg agggttattt cgggaaaatc aatcagttta
     3541 tggagcaggt ctattctcag gggactattt atccacccaa ggaaaaggtt tttcaggctc
     3601 tcttgacaac actgcttgaa gaagttaagg tggtaattct agggcaagac ccctaccacg
     3661 gaccaggtca agcgcagggc ttgagttttt ctgtacctga ctctatccca gctccaccat
     3721 ccttgcaaaa tatcttgaaa gaattgtcag atgatatcgg agttaagaaa tctcatgatt
     3781 tgacagcttg ggctgagcaa ggagtcttgc ttcttaatgc ttgtttgact gttcctgctg
     3841 gacaggccaa tggtcatgct ggtcaaatct gggagccttt tactgatgct gtgattcagg
     3901 tggtcaatca tctagataga ccagtcgttt ttgtactctg gggagcttat gcacgtaaga
     3961 agaaggcatt agttaccaat cctcatcact tgattatcga atcagcccat ccaagtcctt
     4021 tgtcggttta tagaggattt tggggttcca agcccttttc caaggccaat gcattcttaa
     4081 aagagacagg acaagagcca atcgattggc ttagataagg agaaaatatg cctcagttag
     4141 cgacgatttg ctacattgat aatgggaaag aactgctcat gctccatcgt aataagaaac
     4201 ccaatgatgt ccatgaaggg aaatggattg gtgtgggtgg taagctagag agaggagaga
     4261 cgccccagga atgcgcggta cgtgaaatcc ttgaagaaac agggctcaaa gccaagccag
     4321 ttctaaaagg tgtcatcact tttcctgaat ttacaccaga tttagactgg tacacctatg
     4381 tttttaaggt gacggagttt gagggcgact tgattgactg caatgagggg atgctagaat
     4441 gggttcccta tgatgaggtt ttgagcaagc cgacttggga aggtgaccac acctttgttg
     4501 agtggctttt agaggataaa cccttctttt cagccaagtt tgtttatgat ggggataaat
     4561 tgttggatac ccaagttgat ttctatgaat aaaggagaaa gcagatgcta ctaatcaaaa
     4621 atggtcgtgt aatggatccc aagtctggtt tggatcaagt ttgtgatgtc ttagttcaag
     4681 atgggaaaat tatcaaaatt gcgcctgaga tcacggaaga aggagcagaa acgattgatg
     4741 ctactggtca tgtggttgct cctggtttgg tcgatattca tgtgcatttc cgtgaacctg
     4801 gtcaaacaca taaagaagac attcatactg gtgccctagc agccgctgca ggtggtttta
     4861 ctactgtcgt catgatggct aataccagtc caaccatttc agacgtggag actttgcaag
     4921 cagttctcca gtcagctgcc aaagagaaga ttaatgtcaa gacagttgcg accattacta
     4981 aaaactttaa tggtaaaaac ttgactgact ttaaggcact tttagaagct ggtgcggttg
     5041 gtttctctga tgacggtatt ccgcttgaga gcagtaagat tgtcaaggaa gccatggagg
     5101 aagccaaaaa actcaatacc tttatcagcc ttcatgagga agatccaggt ttgaacggtg
     5161 ttcttggctt taatgaaaat attgctagag aacatttcca tatctgtggt gctactgggg
     5221 tggctgagta cgctatgatg gcgcgtgatg tcatgattgc ctatgcaact aaagcccatg
     5281 ttcacatcca gcatttgtct aaggaagaaa gtgttaaagt agtggagttt gctcaggggt
     5341 taggtgcaga agtcacagca gaagtagcac cacagcattt ctctaagaca gaagcacttc
     5401 ttttaacaca aggtagcaat gctaagatga atccaccgct tcgtttggaa tcagaccgtc
     5461 gtgccgttat cgaaggtctc aaatcaggtg tcatcacagt tattgcgact gaccacgcgc
     5521 ctcatcatgt agatgaaaaa aatgttgagg atattaccaa agcgccatct ggtatgactg
     5581 gcttagaaac atccctgtct ctcggcttga cctatttagt agaagctggt gagttgagct
     5641 tgatggaatt acttgaaaaa atgacataca acccagccaa gctttacaac tttgaagcag
     5701 gttacttggc tgagaatggt ccagcagata tcactatttt tgctgccaag gctgaccgcc
     5761 ttgtggactc ccattttgct tccaaagcag ctaattcacc attcatcggt gaaaccttaa
     5821 aagggcaggt taaatatacc atctgtaagg gacaaatcgt ctatcaagct tgataggagt
     5881 tagaatatga attattttcg aaaacgtagg gagagacaag cgaaatctaa tagtggaatt
     5941 tactgtcccg cagctaatcg ttccaaaatt caatatgaaa ctaaagaaaa agctgaccat
     6001 gcggttcaat atgataacgg aggtttagtt agaagctatt attgtcgtac atgtgcgtgt
     6061 tggcatacta cttcaaaatc taataaacct ccgctaagtt tgaaaaacta tggtctgaaa
     6121 ctctttggtt tagataaatc agaatagaaa gagaagtatg tataagacaa agtgtttacg
     6181 agagaagtta gtattatttt taaaaatttt cttcccaatc ctgatctacc aatttgccaa
     6241 ttattctgcc tcttttgttg atactgcaat gacaggtcaa tacaacacta tggacttggc
     6301 tggtgtatct atggcaacca gtatctggaa tcctttcttt gcatttctaa cagggattgt
     6361 gtcagccttg gtgcctatca ttggtcacca tcttggtcga ggcaaaaagg aagaagttgc
     6421 gtctgatttt taccaattta tttatttggc cttgggccta tctgtggtct tgctggggat
     6481 ggtacttttc ttggcaccaa caatcttgaa tcatattggg ttagaagcag cagtagcggc
     6541 agtagcggtt cgctatcttt ggtttttatc tatcgggatt atccccttgt tgctctttag
     6601 cgtcattcgt tccttgctgg attcgctggg cttgaccaaa ctgtccatgt acctcatgct
     6661 tttgttactc cctctcaata gcggatttaa ctatctcttg atttacggtg cctttggtgt
     6721 tccagaactg ggaggggctg gtgctggttt aggaacatcc ttggcctact gggtcttgct
     6781 tgggatttct gttctggttt tatttaaaca ggagaagctc aaagccttac accttgagaa
     6841 acgaattcca cttaatatgg ataaaattaa ggaaggagtt cgtttaggtc tgcctattgg
     6901 gggaactgtc ttcgcggaag tggctatctt ttcagtggtt ggcttgatta tggctaagtt
     6961 ttcgtccttg attatagcta gtcaccagtc agctatgaac ttttcaagtc ttatgtacgc
     7021 ctttcctatg agtatctcat cggctatggc tattgtcgtt tcctatgaag tgggagccaa
     7081 gcgatttgat gatgcgaaaa cctatattgg tctaggaaga tggactgccc tcatttttgc
     7141 ggccttcacc ctaaccttcc tttacatttt taggggaaat gtggccagtc tttatggtaa
     7201 cgacccaaaa tttatcgatt tgacagcgcg ttttttaact tatagtcttt tcttccagtt
     7261 agcagatacc tttgcggcac cgcttcaggg aattttgcgg gggtataagg atacagttat
     7321 tcctttttac cttggtttgc ttggttattg gggcgtagca atccctgtgg ctacgctatt
     7381 tgattcccta acagattttg gagcttattc ttactggatc ggcttgatta ttagtttgat
     7441 tgtgagtggg gcgctctacc gttggcgttt aactgtgatt atgaagagat ttgaatcttt
     7501 agcaaaatcc aaacgctaaa aagtttgtga aaaaatatcc ttgacaagaa aatcccttgt
     7561 tttcacgggg ttttcttttt tattttgtga aatttctttt agaaaaactt tgacagtatt
     7621 tgccaaaagc ggtaaaatag taaggtaaga agaaagttag aaaggcaaga agatgtcaac
     7681 tttagataaa aatcttttgc tagaaatgtt ccgtaagatg gaagaaatcc gtcgcatgga
     7741 cttaaaaatt gcacaattag taaagaaagg gaaagtgcca ggaatgacgc acttttctgt
     7801 tggtgaagag gcagctaacg tgggggctat gttagctctc aatccagatg atctgattac
     7861 ctcaaaccac cgtggacacg ggcaagctat tgctaaaggg attgacctca acggaatgat
     7921 ggctgaaatc cttggtaaat acactggaac ctgtaaaggg aaaggtggat ctatgcatat
     7981 cgctgacctt gatgctggga accttggtgc caatggtatc gtaggtggtg gtatggggat
     8041 cgctgtcggt gcagccctca gtcagcaaat gcaaaatacc ggtaaaatcg ttgtctgctt
     8101 ctttggagat ggtgcgacca atgaaggtgt tttccacgaa gcagtgaaca tggcttctat
     8161 ctggaacctg ccagtcattt tctattgcat taacaacggt tacggtatct ctgcggatat
     8221 caagaaaatg accaatatag aacatatcca tcaacgtagc gccgcttatg gaattcctgg
     8281 aatgttcatc gaagacggta acaatgtcat cgatgtctat gaaggatttc agaaagctgt
     8341 agaccatgtt cgcagtggca atggtccagt cttgattgaa agtgtaactt atcgctggct
     8401 cggtcactca tcatctgacc ctggtaaata tcgtacgcgt gaagaagtgg aattgtggaa
     8461 acaaaaagat ccaatcgaaa acctccgcaa ttaccttatt gaaaataaca ttgcaagtgc
     8521 cgaagaattg gaagaaatcc aagcgcaagt aaaggaagca gtagaagctt ctgttaaatt
     8581 tgcagaggaa agcccattcc caccgcttga atcagccttt gaagatattt acgcagacta
     8641 aggagaaaga gataaaatgg aaacaaaaac aatgtccttc cgtgacacca ttatccttgc
     8701 tatgtctgag gaaatgcgtc gcgatgaaaa tgtgttcttg atgggagaag acgtcggtgt
     8761 cttcggagga gacttcggta cttctgttgg aatgcttgaa gaatttggtc cagaacgtgt
     8821 ccgtgactgt ccgatttctg aagctgccat ctctggagct gcagcaggag cagccatgac
     8881 aggacttcgt ccaatcgtcg atatgacctt catggacttc tctgttattg ccatggacaa
     8941 tatcgtcaac caagctgcta aaacacgtta catgtttggt ggtaaaggtc aggttccaat
     9001 gactgttcgt tgtgcagctg gtaacggagt tggctctgcc gcccagcact cacaatctct
     9061 agagtcttgg tttactcaca ttccaggact taaggttgtg gcacctggaa cacctgcgga
     9121 catgaaaggc ctgcttaagt cttctatccg tgataacaac ccagttatta tacttgagta
     9181 caagtcagaa tttaaccaaa aaggggaagt gccagttgat ccagactaca caattccact
     9241 tggggttggt gaaatcaaac gccaaggaac ggatgtaaca gttgttactt atggaaaaat
     9301 gcttcgccgt gtggttcaag ctgctgaaga attagcagaa gaaggaattt cagttgaaat
     9361 tgttgaccca cgtactcttg ttccgcttga taaggatatc atcataaact cagtgaagaa
     9421 gactggtaag gttattctgg tcaacgatgc ccacaaaaca agtggctata tcggtgaaat
     9481 ttcagctatt atttcagaat cagaagcatt tgactatcta gatgcaccaa tccgccgttg
     9541 tgcaggagaa gatgtgccga tgccttatgc acaaaaccta gaaaatgcaa tgattccaac
     9601 agttgaaagt atcaaagatg caatccgaaa aacttataac aaagaataga gttacataag
     9661 ataagttatg taaataaaat aaagctgacg agaaactttg tatcttttag gtgcagagct
     9721 cttcgtcatt aatatcttag attagagcac gggctaaaag atcggaaaaa agataaatct
     9781 ccttggcttc atcgaagcca tcgtcgattt cctatttttc agtcgctttt gacgcccttt
     9841 gtatcataaa tagaattgga gaggtcatgg ctgatgacaa gctaagagcg actcctgcag
     9901 ctagaaagtt agcggatgat ttagggataa acctctacga cgtttctggc tcaggtgcaa
     9961 acggtcgtgt ccacaaagaa gacgtggaaa cttataaaga cacaaacgtg gttcgcattt
    10021 cgccacttgc aaaacgaatt gccctcgaac ataacattgc ttggcaggaa atccaaggaa
    10081 ccggtcatcg tggtaaaatc atgaagaagg atgttttggc cctgcttcct gaaaatatcg
    10141 aaaacgatag catcaaatct cctgctcaga ttgaaaaagt ggaagaagtc cctgataacg
    10201 taacaccata tggtgaaatc gagcgtattc caatgacacc aatgcgtaag gttattgccc
    10261 aacgcatggt tgaatcttac ttgactgcgc caaccttcac cctcaactat gaagttgata
    10321 tgactgaaat gttggctctt cgtaagaagg ttcttgagcc aatcatggaa gcaactggga
    10381 agaagactac tgtaacagac cttctttcgc ttgcagttgt taagactctt atgaaacacc
    10441 catacatcaa cgcttcattg acagaagatg gcaagactat tatcactcac aactatgtca
    10501 atcttgccat ggcagttggg atggataatg gattgatgac acctgttgtt tacaatgctg
    10561 agaagatgag tctttcagaa ctggtggtag cctttaagga tgttattggc cgtaccttgg
    10621 atggtaaatt ggctccaagt gagttacaaa attcaacatt cacaatcagt aatttgggaa
    10681 tgtttggtgt tcagtccttt ggtccgatta ttaaccaacc caactcagct atccttggtg
    10741 tcagttcgac aatcgagaag ccagttgtcg tcaatggtga aattgtgatt cgcccaatca
    10801 tgagtttagg attaaccatt gaccaccgtg tcgtagatgg tatggctggt gctaagttta
    10861 tgaaggactt gaaagagttg attgaaacgc caatctcaat gttaatttaa gagcaagagt
    10921 attcattttt aagatataga taaaggaagg aagacatggc cttagaagta attatgccaa
    10981 aagccggcgt ggatatgaca gaaggacaaa tcgtccaatg gaataaaaaa gtcggagaat
    11041 ttgtaaaaga aggagaaatc cttttggaaa tcatgactga taaagtcagc atggaattgg
    11101 aagccgaaga agatgggtac ttgattgcca ttctcaaagg agatggtgaa actgtccctg
    11161 taacggaagt tatcggttac cttggtgaag aaagggaaaa catcccaaca gctggagcag
    11221 catcgccaga agctagccct gtacctgtag caagtacctc aaacgatgat ggtaagagcg
    11281 atgatgcttt tgatatcgtt gtgattggtg gaggtcctgc tggttatgtt gcagccatta
    11341 aagctgccca actcggcggt aaggttgccc ttgttgagaa atctgaactt ggtggaacct
    11401 gcttaaaccg tggatgtatt ccaaccaaga cctaccttca tagcgctgaa attattgaaa
    11461 atatcggtca tgctgcaaat cgtggtatcg tgattgaaaa tcctaacttc actgttgata
    11521 tggaaaaact tttagaaact aaatctaaag ttgttaatac tcttgttggt ggtgttgcag
    11581 gacttcttcg tagctacgga gttgctgttc ataaaggaat tggtacaatc actaaagaca
    11641 agaacgtctt ggtaaatggt tctgaattgc ttgaaaccaa gaaaatcatt cttgctggtg
    11701 gttcaaaagt cagcaagatc aacgtccctg gtatggaatc tccacttgtc atgactagtg
    11761 atgacattct tgaaatgaac gaagtgccag aaagtcttgt tatcatcggt ggtggagttg
    11821 tcggtatcga actcggtcag gccttcatga catttggttc aaaagtgact gttatcgaaa
    11881 tgatggaccg tatagttcca gctatggatg tggaagtttc taagaacctt cgcttgatcc
    11941 ttgagcgtaa aggaatgacc atcttgactg gtactaaact gcaagaaatc attgaggaaa
    12001 atggtcaact tcgtatcaag gttgaaggaa aagacgatat catcgcaagc aaagctcttc
    12061 tttcaattgg tcgtatgcca gaccttgaag gtattggaga ggttgagttt gaattggatc
    12121 gtggttgtat caaggtcaac gaatacatgg aaacttcagt tccaggcatt tatgcaccag
    12181 gtgacatcaa cggtactaag atgttggctc acgcagcttt ccgcatgggt gaagtttccg
    12241 ctgaaaatgc ccttaaagga aatcatgcag ttgccaaatt gaatttgact cctgcagcca
    12301 tctacactct tcctgaagta gcagcagtag gtttgacaga agaacaagcc cgtgagaaat
    12361 acgatgttgc catcggtaag tttaactttg ctgctaacgg tcgtgctatt gcatctgacg
    12421 cagctcaagg tttcgtaaaa gttatcgctg ataagaaata cggagaaatc cttggtgtac
    12481 acattattgg tcctgcagcc gcagaattaa taaacgaagc atcaagcatc atcgaaatgg
    12541 aaatcactgt tgaggaaatg ctgaagacca tccacgggca cccaacttac tctgaagtga
    12601 tgtacgaagc gtttgcagat gttctaggaa tggccatcca ttcacctaag aaaaaataaa
    12661 acgaagatag attagactgg aaggttattt ccagtttcta tcatataaag taaagggata
    12721 tcatgaaata cattatcaat cattcaaatg acactgcttt taatattgcc ttggaagaat
    12781 acgcctttaa acaccttttg gatgaggatc aaatcttcct actttggatt aacaagccat
    12841 ctatcattgt tggtcgtcac cagaacacta tcgaagaaat caaccgtgat tatgttcgcg
    12901 aaaatggtat tgaggtagtt cgccgtatta gtggtggtgg agctgtttac cacgatttaa
    12961 ataacctcaa ctacacgatc atctccaaag aagatgaaaa taaggcattt gacttcaaga
    13021 gcttctcaac tccggttatc aataccctgg ctcaactcgg ggttaaagct gagttcacag
    13081 gtcgtaatga ccttgagatt gatggtaaga agttctgtgg caatgcccaa gcctatatca
    13141 acggccgtat catgcaccac ggttgcttgc tctttgacgt tgatttgtca gtcctcgcaa
    13201 atgcccttaa ggtttcaaaa gataaatttg aatcaaaagg tgtgaaatcc gtccgtgccc
    13261 gtgtaaccaa tattatcaat gaattaccaa aaaaaatcac agtcgaaaaa ttccgtgatt
    13321 tactcttgga atacatgaaa aaagagtacc cagagatgac tgaatacgtc ttttcagaag
    13381 aagaattggc cgaaataaat cgcatcaagg atactaagtt tggaacttgg gactggaact
    13441 atggtaaatc acctgaattt aacgtccgtc gtggaaccaa attcactagt ggtaaggttg
    13501 aagtctttgc taacgttact gaatcaaaaa tccaagacat caagatttat ggtgacttct
    13561 ttggtattga agacgttgct gcagtagaag ctgtccttcg tggggtgaaa tacgaacgtg
    13621 aagatgttct taaggcgcta aaaaccattg atatcacacg ctacttcgct ggtattagcc
    13681 gtgaagaaat cgctgaagcg gtagttggat aaatcaaaaa gaagttggcc aactaaccaa
    13741 cttctttttt tgatatttat ttacataatt taaaatacgt aaaatcataa actatccaga
    13801 gcattctttt gttcatcact aacaatatgg gtatagaggt cagtgacttg tgtgctagca
    13861 tgtcctagtt ggtgactgac taaaacttgt gatttagtcg catcatagag cctagttgct
    13921 agtgtatggc gcagtttatg gggtgttaca cgcactttaa aatcctctga gtatttagca
    13981 accattttct caacgctaga agcatcgata cgattaggaa cacctctgta gagagttaaa
    14041 aaaagggctg tatctgtttt ttccgtttta tagcgttgat tccgaatgac cagataattc
    14101 tctaaataag gtttagcaaa agcagcgaca ttgactgagt cacgtttgcc accttttcga
    14161 gtaacatcaa taaccatcat ttttagattg agatctctta gatctagatt aacagcttca
    14221 gataagcgaa caccagatgc caagagaagg gcaataatgg ctaaatctcg ttctttattt
    14281 ttgttgaatg atgagagagc tcgatttgaa agctgttgtg ggtgctcttg atcgatataa
    14341 gttagaaaac cttctgtttc atcacctaga aagagttttt gcttgatatt ttcagctctg
    14401 gcagcaaggg tttctttctt tttcttggtt gaaacttttt tcattacatt acgatagaaa
    14461 taaggttccc cctgatcgtt ttcaacctcc tcggttagat acttgtaaag actagaaagt
    14521 gctgataagg ttcgattgat agttgtctgt gaaacacctt gttttgttgt attagcattc
    14581 agcaagggac gttcacgtag ataaaggata aaggattcca tgtctttctt agacatattt
    14641 tccaaaactg ataaaggaat atcggatatt ttatcagcgt ttgaaatacc agactccaaa
    14701 acccagctga aaaatcggtc atattcctta aggtattcgt acagggttgt aaaactgtag
    14761 ggcacagcca gtttagattg gtagtattcc agaacatacc agggcatgag ttgttttagt
    14821 ttgtcgattc gttccagtaa aatctcacgt ttcatgtatt tcctccataa ttaagtatac
    14881 tagtagtata gcatagactt gtatattttt caagaaaatt atagtttttc tgaaagatgt
    14941 tataggagtt tttcaaagtg ataaaaagac ttagacctac aaacaaaaca gaataaggaa
    15001 aataataaaa atctcaccca ctaaacttct gttaatacta cataactgaa gagctatata
    15061 aggagctatg agatacaaaa cacattgtat aatagattga aactagaata gtacatctga
    15121 acttctaaaa cattgctaga aattgatttg attgtcctga ttgttttatc ctatttctat
    15181 ttcatttcac tatataattg ctattgtttt ctttttca
//
LOCUS       DAVKQL010000049        14486 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVKQL010000049 DAVKQL010000000
VERSION     DAVKQL010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14486
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.049"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(1..692)
                     /locus_tag="VW561_001561"
                     /pseudo
     CDS             complement(1..692)
                     /locus_tag="VW561_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            687..941
                     /locus_tag="VW561_001562"
     CDS             687..941
                     /locus_tag="VW561_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU9943798.1"
                     /translation="MHRTTIPYFVSQEVYWKVRNIEAEAIRRNCERGAIFSGKIKYHE
                     DSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            1186..2520
                     /gene="trmFO"
                     /locus_tag="VW561_001563"
     CDS             1186..2520
                     /gene="trmFO"
                     /locus_tag="VW561_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEU9943799.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNNGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            2604..3341
                     /locus_tag="VW561_001564"
     CDS             2604..3341
                     /locus_tag="VW561_001564"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU9943800.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            3350..3907
                     /gene="frr"
                     /locus_tag="VW561_001565"
     CDS             3350..3907
                     /gene="frr"
                     /locus_tag="VW561_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU9943801.1"
                     /translation="MANIIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            3967..4821
                     /gene="cvfB"
                     /locus_tag="VW561_001566"
     CDS             3967..4821
                     /gene="cvfB"
                     /locus_tag="VW561_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEU9943802.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSFKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            4830..5045
                     /locus_tag="VW561_001567"
     CDS             4830..5045
                     /locus_tag="VW561_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEU9943803.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            5131..6099
                     /locus_tag="VW561_001568"
     CDS             5131..6099
                     /locus_tag="VW561_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU9943804.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(6077..6256)
                     /locus_tag="VW561_001569"
     CDS             complement(6077..6256)
                     /locus_tag="VW561_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943805.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLKPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            6292..6792
                     /locus_tag="VW561_001570"
     CDS             6292..6792
                     /locus_tag="VW561_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9943806.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGFDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIV
                     EQSLED"
     gene            6795..7054
                     /locus_tag="VW561_001571"
                     /pseudo
     CDS             6795..7054
                     /locus_tag="VW561_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(7421..7747)
                     /locus_tag="VW561_001572"
     CDS             complement(7421..7747)
                     /locus_tag="VW561_001572"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943807.1"
                     /translation="MVDIAVDQGGLIETADRVTTHDEPVYEKHGVLHYAVANIPGAVA
                     RTSTIALTNVTLPYIEALAGKGFAQAISEDEGLRQGVTTYQGYLTSLPVAQGLNRDYT
                     DINDLV"
     gene            complement(7693..8106)
                     /locus_tag="VW561_001573"
     CDS             complement(7693..8106)
                     /locus_tag="VW561_001573"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEU9943808.1"
                     /translation="MGAHFLTKQAGGSGVLPGGVPGVPKGKVTIIGDGVVGTHAARIA
                     LGLGAQVTILDISAKRLSVLEEVFGSQIQTLISNSFNIEASMRDADVVIGAILIPGAK
                     APELVTDEMVKQMRPGSVSLTLLLTKVALSKQLTV"
     gene            complement(8107..8532)
                     /locus_tag="VW561_001574"
     CDS             complement(8107..8532)
                     /locus_tag="VW561_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEU9943809.1"
                     /translation="MLIGIPKEIKNNENRVALTPAGVHSLVSRGHRVLIETNAGLGSG
                     FTDADYQKQGAEIVATAGEAWAAELVVKVKEPLSSEYGYLRDDLLLFTYLHMAAAPEL
                     ADAMLAAKTTGIAYETVRDNQGQLPLLVPMSEVAGRMAV"
     gene            8709..9278
                     /locus_tag="VW561_001575"
     CDS             8709..9278
                     /locus_tag="VW561_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9943810.1"
                     /translation="MESIFVKLAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKSNGQFIGTIDLHKIDSVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKDNPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            9346..9996
                     /locus_tag="VW561_001576"
     CDS             9346..9996
                     /locus_tag="VW561_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEU9943811.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     VEVAAVSKDLVSEKEVNKEEKEEPVEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSISKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD"
     gene            9980..12220
                     /locus_tag="VW561_001577"
     CDS             9980..12220
                     /locus_tag="VW561_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEU9943812.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCL
                     FIQFPWKSAGKVLIICGIFGFWFVFQNWQQSQASQNLADSVERVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVTSRPLVFGQPNAWFLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     LEVTKAFHVGEILVSKGSLKQKEFVAELQTTQTKVRSMTVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGVDSW
                     KIESVR"
     gene            12399..12587
                     /locus_tag="VW561_001578"
     CDS             12399..12587
                     /locus_tag="VW561_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943813.1"
                     /translation="MTATKSYKYDWNTVLEYSTNYHDHQYAWIPELSRYYSYSEYKVG
                     GGWNYARYKVINYYTGSY"
     gene            12619..13206
                     /locus_tag="VW561_001579"
     CDS             12619..13206
                     /locus_tag="VW561_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9943814.1"
                     /translation="MLNLTHVTLKTRQVILQDADFTFKKGRIYGLLAINGSGKTTLFR
                     AMSKLLPLSSGHIAVPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            13210..14394
                     /locus_tag="VW561_001580"
     CDS             13210..14394
                     /locus_tag="VW561_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9943815.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILLLFASVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLPKQIDNVNLNWSMGMVLLPCLIIFLLVGILFIESWGSSRKKEVFN
                     RS"
BASE COUNT         4280 a         2719 c         3262 g         4225 t
ORIGIN      
        1 atttcgatag gttgttgaaa acattttaaa cgtttttact ttggcaaaga tgttctcaac
       61 cttgcttctc tccttagata gcgcatggtt acaggcttta tcttcagctg ttagcggctt
      121 gagtttgctg gatttacgtg gagtttgtgc ttgaggatat atcttcatga gcccttgata
      181 accactgtcg gccaagattt taccagcttg tccgatattt ctgcaactca ttttgaacaa
      241 cttcatatca tgactatagt tcacagcgat atccaaagaa acaattctcc cttgacttgt
      301 gacaatcgcc tgagccttca tagcgtggga tttcttttta cttccgtcac atcaatcatt
      361 accgtgtcct cagaactgag aggagttctt gaaatcgtaa caccactttg aacaagagtt
      421 acttcaaccc attggctccg acggattaag ttgctttcgt gaataccaaa atcagccgca
      481 atttgttcat aagttcgata ttctcgcaca tattgaagag tgaccataag aaggtcttct
      541 aggcttaatt taggttttcg tccacctttt gcgtgtttaa gttgataagc tgtttttaat
      601 acagctaata tctcttcaaa agtcgtgcgc tgaacaccaa caagacgctt aaatcgtgca
      661 tcagttagtt gtttacttgc ttcatcattc atagaactac tataccatat tttgtttcgc
      721 aggaagtcta ttggaaagta agaaatattg aagctgaggc tattagaaga aattgtgagc
      781 gtggtgctat tttttcaggt aaaataaaat atcacgaaga ttcacagttt aaaggagatc
      841 actatgttga atgttatgct gttttagata atacggttat agcaagagat agaataacag
      901 tccctatcga tccgttatgt ggaaaagatt ttatagagta gcatataatt gattcttaac
      961 tggaatactc actatctctt tacatcaaga aaatgactaa acagggaagt ttgccttctt
     1021 cccttttttt gttatactag tagaagaaaa aatagaaaga tttgtgggag tgaaaaagcc
     1081 ctgtggactt tttcagcctg agccaagaaa ctcgaaagct tgtaagtctg attggctttt
     1141 tcaatgtgaa tcttaacttc acactcccaa agaggtatta gtgtcgtgtc tcaatcttat
     1201 atcaatgtta tcggtgctgg tttggcaggt tctgaagcag cttaccaaat cgcagagcgt
     1261 ggtattccag ttaaactata tgaaatgcgt ggtgtcaagt ctacacccca gcataaaaca
     1321 gacaattttg ctgagttggt ttgttccaat tctttgcgtg gggatgcttt gacaaatgca
     1381 gttggtcttc tcaaggaaga aatgcgtcgc ttgggttctg ttatcttgga atctgctgag
     1441 gctacccgtg ttcctgccgg tggagccctt gcggtggatc gtgatggttt ctctcaaatg
     1501 gtgaccgaaa aagtggtcaa ccaccccttg attgaagtgg tgcgtgatga aattacagaa
     1561 ttgccaacag atgttattac agttgtggcg actggtccct tgactagcga tgccctggct
     1621 gaaaagattc atgcccttaa taatggcgat ggcttctatt tctacgatgc agcagcgcct
     1681 attatcgatg tcaacactat cgatatgagt aaggtctatc tcaaatctcg ttatgataag
     1741 ggagaagcag cctatctcaa tgctccaatg accaagcaag agtttatgga tttccatgaa
     1801 gccttggtca atgcggaaga agcaccgctc aattcttttg aaaaagaaaa gtactttgaa
     1861 ggatgtatgc caatcgaagt tatggccaaa cgtggcatta aaactatgct ttatggccct
     1921 atgaagccag tcggtcttga gtatccggac gactacacag gacctcgtga tggagaattt
     1981 aaaacacctt atgcggttgt gcaacttcgt caggataatg cagctggtag cctatacaat
     2041 attgttggtt tccagaccca cctcaaatgg ggagaacaaa agcgtgtctt ccaaatgatt
     2101 ccgggtcttg aaaatgcgga gtttgtccgt tatggtgtca tgcatcgcaa ttcttacatg
     2161 gattcaccaa atcttcttga gcagacttac cgttccaaga aacaaccaaa tctcttcttt
     2221 gctggtcaga tgacgggggt ggaaggctat gttgagtcgg cggcttcggg cttagttgcg
     2281 ggaattaacg cagctcgtct cttcaaggaa gaaagcgagg ttattttccc cgagacgaca
     2341 gcgattggaa gcttagctca ttacattacc catgccgaca gcaaacattt ccaaccaatg
     2401 aatgtcaatt ttgggatcat caaggagttg gaaggcgagc gtatccgtga taagaaggct
     2461 cgttatgaaa aaattgcaga gcgtgccctt gccgacttag aggaattttt gactgtctaa
     2521 tttttttgaa agaattgctc atgatactat aaaaatctta gaaattgtga taaaataggt
     2581 aggatgaaag aaggagagtg aaaatggcga atcccaagta taaacgtatt ttaatcaagt
     2641 tatcaggtga agcccttgcc ggtgaacgtg gcgtagggat tgatatccaa acagttcaaa
     2701 caatcgcaaa agagattcaa gaagttcata gcttaggtat cgaaattgcc cttgttattg
     2761 gtggaggaaa tctctggcgt ggagaacctg cagcagaagc aggtatggac cgtgttcagg
     2821 cagattacac tggaatgctt gggactgtta tgaatgctct tgtgatggca gattcattgc
     2881 aacaagttgg ggttgatacg cgtgtacaaa cagctattgc tatgcaacaa gtggcagagc
     2941 cttatgtccg tggacgtgcc cttcgtcacc ttgaaaaagg ccgtatcgtt atctttggtg
     3001 ctggaattgg ttcaccatac ttctcgacag atacaacagc ggcccttcgc gcagctgaaa
     3061 tcgaagcaga tgccatcctc atggctaaaa atggcgtcga tggtgtgtac aatgccgatc
     3121 ctaagaagga caagacagcc gttaagtttg aagaattgac ccaccgtgat gttatcaaca
     3181 aaggtcttcg tatcatggac tcaacagcct caaccctctc aatggacaac gacattgact
     3241 tggttgtctt caacatgaac caatcaggca acatcaaacg tgtcgtattt ggtgaaaata
     3301 tcggaacaac agtttcaaat aatatcgaag aaaaggaata agaaagaata tggctaacat
     3361 aattattgaa aaagctaaag agagaatgac ccagtctcac caatcacttg ctcgtgaatt
     3421 tggtggtatc cgtgctggtc gtgccaatgc aagcttgctt gaccgtgtac atgtagaata
     3481 ctatggagtc gaaactcctc ttaaccaaat cgcttcaatt acgattccag aagcgcgtgt
     3541 tttgttggta acaccatttg acaagtcttc attgaaagac atcgaacgtg ccttgaacgc
     3601 ttctgatctt ggtatcacac cggctaatga cggttctgtg attcgcttgg ttatcccagc
     3661 tcttacagaa gaaactcgtc gtgaccttgc taaagaagtg aagaaggtcg gcgaaaatgc
     3721 taaagtggct gtccgcaata tccgtcgcga tgctatggac gaagctaaga aacaagaaaa
     3781 agcaaaagaa atcactgaag acgaattgaa gactcttgaa aaagatattc aaaaagtaac
     3841 agacgatgct gttaaacaca tcgacgacat gactgctaac aaagagaaag aacttttgga
     3901 agtctaaaaa taaacagaaa aactcagttg gcattgctgg ctgagtttta ttcgaaagaa
     3961 ggaaatatga atacaaatct tgcaagtttt atcgttggac tgatcatcga tgaaaatgac
     4021 cgtttttact ttgtgcaaaa ggatggtcaa acctatgctc ttgctaagga agagggccaa
     4081 catacagtag gggatacggt caaaggtttt gcatacacgg atatgaagca aaaactccgc
     4141 ctgacaacct tagaagtgac tgccactcag gaccaatttg gttggggacg tgtcacagag
     4201 gttcgtaagg acttgggtgt ctttgtggat acaggccttc ctgacaagga aatcgttgtg
     4261 tcactcgata ttctccctga gctcaaggaa ctctggccta agaagggcga ccaactctac
     4321 atccgtcttg aagtggataa gaaagaccgt atctggggcc tcttggctta tcaagaagac
     4381 ttccaacgtc tcgctcgtcc tgcctacaac aacatgcaga accaaaactg gccagccatt
     4441 gtttaccgtc tcaagctgtc aggaactttt gtttacctac cagaaaataa tatgcttggt
     4501 tttattcatc ctagcgagcg ttacgcagag ccacgtttgg ggcaagtatt agatgcgcgc
     4561 gttattggtt tccgtgaagt ggaccgcact ttgaacctct ccttcaaacc acgctccttt
     4621 gaaatgttgg aaaacgatgc tcagatgatt ttgacttatt tggaaagcaa tggcggtttc
     4681 atgaccttaa atgacaagtc atctccagac gacatcaagg caacctttgg catttctaaa
     4741 ggtcagttca agaaagcttt aggtggtctt atgaaggctg gtaaaatcaa gcaggaccag
     4801 tttggtacag agttgattta gggaggctta tgagaaaatc attttacact tggctcatga
     4861 ccgagcgcaa tcctaaaagt aacagtccca aagcaatttt ggcagacctc gcttttgaag
     4921 aggcagcttt tccaaaacat acagatgatt ttgatgaggt cagtcgcttt ttggaggagc
     4981 atgccagttt ctcttttaac ctaggagatt ttgacagcat ttggcaggaa tatctagaac
     5041 actagcattt attcattggg tttgggctag taatttctcc atccctctgc tataataaaa
     5101 agaaataaaa ggattagaga ggttctttat ttgaaggaac attcaataga cattcaactg
     5161 agtcatccag atgacctgtt tcatcttttt ggttccaatg agcgccatct tcgtttgatg
     5221 gaagaagagc tggatgtggt gattcatgct cgtacggaga ttgtccaggt tttgggagaa
     5281 gagtctgctt gtgaggaagc ccgtcaggtt attcaagctc tcatggtctt ggtgaaccgt
     5341 gggatgaccg ttggtacgcc agatgtggtg actgcaatta gcatggtcaa aaacgatgaa
     5401 attgataagt ttgtcgccct ttacgaagaa gaaattatca aagataatac tgggaaaccg
     5461 atccgcgtca aaaccttagg tcaaaaactt tatgtggaca gtgtcaaaca gcatgatgtg
     5521 acctttggaa ttgggcctgc agggacgggg aagacctttc ttgcagttac tttggcagtg
     5581 actgccctta aacgtgggca agtcaagcgg attatcttga cacgtccagc ggtggaagca
     5641 ggagagagtc taggttttct tccgggtgat cttaaggaga aggtagatcc ttaccttaga
     5701 cctgtttatg atgccttgta tcagattctg gggaaagacc aaacaactcg actcatggag
     5761 cgtgaaatta tcgagattgc gccccttgcc tatatgcgtg gacggacctt ggatgatgcc
     5821 tttgtcattc tcgatgaggc gcaaaatacg accatcatgc agatgaagat gttcttgact
     5881 cgtttaggtt ttcattctaa gatgattgtc aatggagata ttagtcagat tgacctgcca
     5941 cgtaatgtca agtccggttt gattgatgct caagagaaac tcaagaacat ccatcagatt
     6001 gactttgttc atttttcagc caaggatgtg gttcgccatc ctgttgtcgc tcagattatc
     6061 cgagcctatg aatattctac tgaagttgca cacgactgat tttgaggaag ttcgcctgca
     6121 aaagaatata cttttgagag aggaaaatcc agttgtatag gctaaaggtt ttatccaaag
     6181 gtttgagaca acgattaggc acgatggaaa gaacttttat gtggctgatg acgatcagtg
     6241 catcttcctg tgtcataatc acaggacaca agaaagtagg aatttgaaaa gatgattgac
     6301 caactatcta agtattacag ttgtaggata ctaactgaaa aggatattcc aagtatttta
     6361 tctttatatg aaagtaatcc tctgtatttt cagcattgtc caccagagcc aaattttgca
     6421 actgtaaaag aggacatgct ttgtctacct gaaggtaaag ctaaggctga taagtttttt
     6481 gttggatttt ggaatggatt tgaccttgtg gctgttatgg attttgtcta tgcatatcct
     6541 gatgaggaga ctgtttttat tggtttgttt atggttgatc aagcctatca gaggaaaggg
     6601 attggtagtc atattgtgac agaagcacta gcttattttg ctaagaactt tcgaaaggca
     6661 cgtttggctt atgttaaggg aaatccgcaa tctcagcatt tttgggaaaa gcagggcttt
     6721 aaatcaattg gatgcgaggt taagcaagaa ctctatacgg ttgttatcgt tgaacagagc
     6781 ctagaagatt agaaatggca tcaagtaaga actatttgga atttgttttg gaacaattat
     6841 caggattaga tgatgtgact taccgttcca tgatggggga gtatattctt tacttccgcg
     6901 gcaagattat tggcggcatt tatgacgatc gcttttagtt aaacccgtgc aagcagtctt
     6961 agataagatt gaccaatctt cttttgagtt tccatacaaa ggtgccaaag aaatgatttg
     7021 agtggaagaa cttgataata agatgtttct ataagacctg attttagcta tgtataacca
     7081 actgccaacg cccaaaccta aaaagaaaaa gcaagggtga acgaagtaaa aaagaagtct
     7141 gctaaggccc tgtctttgca cgggtaaaat tttatatata aaaagaagct gggactaaag
     7201 agctcagctt cctttggttt atataattgt cattacaaga cgaagtggtt gggcgaaact
     7261 ctgttgactt tattcaattt agagtttctt atgcacaatt gagtctggaa cgaaagtctc
     7321 cagttgcaaa gtatacagta caataaacca acgatgtaat agctgatgac acaaagcaca
     7381 gtgggtagga cttgcgaagt cacccttttc ttttcaaaat ttatactaaa tcattgatat
     7441 cagtgtagtc acgattaagt ccttgagcaa ctggtaggct agtcaagtaa ccttgataag
     7501 tggtcacacc ttgacgcaag ccttcatctt cagagattgc ttgtgcgaat cctttgccag
     7561 ccaaagcttc gatataagga agagtgacat tggttagggc gatggttgaa gtgcgggcaa
     7621 ccgcaccagg gatattggca acggcatagt ggagaacacc gtgtttttca tagacgggtt
     7681 catcgtgcgt tgtcacacgg tcagctgttt cgataaggcc accttggtca acagcaatgt
     7741 caacgataca gagcctggac gcatttgttt gaccatctca tctgtcacca attccggtgc
     7801 ttttgcacca gggatgagaa tggctccaat caccacatca gcatctctca tacttgcttc
     7861 aatgttgaat gaattagata taagagtttg aatttgactt ccaaagactt cttctagaac
     7921 tgagagacgc ttggcactaa tatctaaaat agtcacttga gcaccaagac caagggcgat
     7981 gcgggcagca tgtgtaccga cgacaccatc accgatgata gttacttttc cttttggaac
     8041 acctggtaca ccaccaggta gaacaccaga gccaccagct tgcttagtaa ggaagtgagc
     8101 tccgatttaa acagccatac gacctgcaac ctcactcata ggaacgagga gcggtagttg
     8161 tccttgattg tcacgaacag tttcataggc aattcctgtt gtttttgctg ctaacatagc
     8221 atctgctaat tctggagcag cggccatgtg caagtaggtg aagagaagaa gatcgtcgcg
     8281 caagtaaccg tattcagaac ttaaaggttc ttttactttc acaaccaact ctgctgccca
     8341 agcttcacca gcagtagcga caatctcagc tccttgcttt tgatagtcag catcagtaaa
     8401 gccagaaccg agaccagcat ttgtttcgat aaggacacga tgaccacgac taactaagct
     8461 atgaacacct gcaggtgtga gggcgacacg gttttcgtta tttttaattt cttttgggat
     8521 tccgattaac attgagataa cctacctttc aattgacggt cttgttttgg ttgtcacatt
     8581 ccagttcata aatcaaaaat gtgacggttt cattgtatat gaaaccgctt caaaaatcaa
     8641 gaaaaacttg tcatccaaat tttttatgct agactagtga aaatcaagct ttgattggtt
     8701 ggaaaagtat ggaatcaatt tttgtaaaac ttgcccagta tccgtctata gaaacggagc
     8761 gtttattgct tagacctgta actttggatg atgcggaagc aatgtttgac tatgcctcgg
     8821 acaagggtaa tacacgttac acttttccaa ccaatcaaag cttggaagaa accaagaata
     8881 acattgctca gttctatttg gctaatccct tgggacgttg gggaatagaa ctaaaaagca
     8941 atggtcagtt tattggaacc attgacttgc acaagattga ttctgttctt aagaaggcag
     9001 ctattggcta cattatcaat aaaaagtatt ggaatcaagg attaacgaca gaagccaatc
     9061 gtgctgtgat tgagctagct tttgagaaga tagggatgaa taagttgact gcccttcacg
     9121 ataaggacaa tcctgcgtca ggaaaggtca tggagaaatc aggcatgcgt ttttcccatg
     9181 cagaaccata tgcttgtatg gaccagcatg aaaaaggccg aatcgtgaca agagttcatt
     9241 atgtcttgac caaggaagac tattttgcaa ataaataagc agttgaaaag aaatttttcg
     9301 actgtttttt cttcctctta cgaataatct aagagaggag aaaatatgga agcaattatc
     9361 gagaaaatca aagagtataa aatcatcgtc atctgtactg gtctgggctt gcttgtaggc
     9421 ggatttttcc tgctaaagcc agctccacaa acacctgtaa aggaaacgaa tttgcaggtt
     9481 gaagttgcag ctgtttccaa ggatttggta tccgaaaagg aagtgaacaa ggaagagaag
     9541 gaagaaccag ttgaacaaga tctaatcaca gtagatgtca aaggtgctgt caaatcgcca
     9601 gggatttatg acttgcctgt aggtagtcga gtcaatgatg ctgttcagaa ggctggtggc
     9661 ttgacagagc aagcagacag caagtcgctc aatctagctc agaaagttag tgatgaggct
     9721 ctggtttacg ttcctactaa gggagaagaa gcagttagtc aacagactgg ttcggggaca
     9781 gcttcttcaa taagcaagga aaagaaggtc aatctcaaca aggccagtct ggaagaactc
     9841 aagcaggtca agggactggg aggaaaacga gctcaggaca ttatcgacca tcgtgaggca
     9901 aatggcaagt tcaagtcagt agacgagctc aagaaggtct ctggcattgg tggcaaaaca
     9961 atagaaaagc ttaaagacta tgttacagtg gattaagaat ttctctattc ccctaattta
    10021 cctgagtttt ctattacttt ggctttatta cgctattttc tcagcatctt atcttgcttt
    10081 gttgggcttt gtttttctgc tagtctgtct ctttatccaa tttccgtgga aatctgctgg
    10141 taaagttcta ataatttgcg gaatctttgg attttggttt gtttttcaaa attggcaaca
    10201 gagtcaagcg agtcaaaatc tggcggattc tgttgaaagg gtacggattt tgcctgatac
    10261 tattaaggtt aatggtgata gtctatcctt tcgtggcaag tctaacggtc gtgctttcca
    10321 agtctattat aaactccagt ccgaggagga gaaagaagcc tttcaagctt taaccgacct
    10381 gcatgagata ggactagaag ggaagctttc ggagccagaa gggcagagaa attttggtgg
    10441 ctttaattac caagcctatc tgaagactca gggaatttac cagactctca atatcaaaac
    10501 aatccagtca cttcaaaaga ttggcagttg ggatatagga gaaaacttgt ccagtttacg
    10561 tcgaaaggct gtggtttgga ttaagacgca ctttccagac cctatgcgca attacatgac
    10621 aggactcttg ctgggacatc tggacaccga ctttgaggag atgaatgagc tttattccag
    10681 tctaggaatt atccacctct ttgccctatc tggtatgcag gtaggttttt tcatgaatgg
    10741 atttaagaaa cttctcttgc gattgggctt gacccaagaa aagttgaaat ggctgactta
    10801 tcccttttcc cttatctatg cgggactaac tggattttca gcatcggtta ttcgcagtct
    10861 cttgcaaaag ctactggctc aacatggggt taagggcttg gataattttg ccttgacggt
    10921 gcttgtcctc tttattgtca tgccaaactt tttcttgaca gcaggaggag tcttgtcctg
    10981 cgcttatgct tttatcctga ccatgaccag caaagaaggg gaggggctca aggctgttac
    11041 tagtgaaagt ctagtcatct ccttgggcat attgcccatt ctatccttct attttgcgga
    11101 atttcaacct tggtctatcc ttttgacctt tgtcttttcc tttctatttg acttgacctt
    11161 cttaccgctc ttgtctattt tatttgtcct ttcctttctc tatccagtca ttcagctgaa
    11221 ctttatcttt gaatggttgg agggcattat tcgcttggtg tcacaggtga caagtagacc
    11281 tctggtcttt ggacaaccca atgcatggtt tttaatccta ttgttaattt ccttggcttt
    11341 ggtctatgat ttgagaaaaa acattaaaaa gctaacggta ttgtgcttat tgattacagg
    11401 gctctttctc ctgaccaagc atccactgga aaatgaaatc accatgctgg atgtggggca
    11461 aggagaaagt attttcctac gggatgtaac tgggaaaacc attctcatag atgtaggtgg
    11521 taaggcagaa tcttataaga aaatcgaaaa atggcaagaa aagatgacga ccagcaatgc
    11581 ccagcgaacc ttgattccct atctcaaaag tcgaggagta gctaagattg accagctgat
    11641 tttgacaaat acggacaagg aacatgtcgg agatttgtta gaggtgacca aggctttcca
    11701 tgtaggggag attttagtat caaaaggcag tttgaagcag aaggaatttg tggcagaact
    11761 acagacgact caaacaaagg tgcgtagtat gacagtaggg gagaacttgc ccatttttgg
    11821 aagtcagtta gaagttctat ctccaaggaa aatgggagat ggaggacacg atgacaccct
    11881 agttctgtat gggaaattct tggataagca atttctcttc acgggaaatt tggaggagaa
    11941 aggagagaag gacttgctga agcactatcc agacttgaaa gtaaatgttt tgaaagctag
    12001 ccaacatggc aataaaaaat catcaagtcc agcctttcta gaaaaactca aaccagagct
    12061 tactcttatc tcagttggaa agagcaatcg aatgaaactc ccccatcagg aaacattgac
    12121 acgactggaa ggtatcaata gcaaagttta tcgaactgac cagcaaggag ctatacgttt
    12181 taagggggtg gatagttgga aaatcgaaag tgttcgatag gaaggataaa tgttgtagat
    12241 tagtgaaata aactaaaaat ttgttgcata ataatgataa aaacggtata atgaaaacgt
    12301 attcaatatt gaggatataa aatcgttaaa aatcagcaaa agttgtttta ttagttagtt
    12361 tataatctat tggtcttctt cagtccagtg tatctgctgt gacagccact aaaagttaca
    12421 agtatgattg gaatacggtt ttggaatata gtaccaacta tcacgaccat cagtatgctt
    12481 ggattccgga attgtctcgt tattacagct attctgagta taaagttggc ggaggctgga
    12541 actacgctcg ttataaggtc ataaactact atactggaag ttattaatcc ttaaagagtg
    12601 agaaaaggag ggctggatat gttaaatctt actcatgtta ccttaaaaac gcgacaagtc
    12661 atcttgcaag atgcggattt tacctttaaa aagggtagga tttatggcct tcttgctatc
    12721 aatggctcgg gaaagacgac actattccga gctatgagca agttgcttcc ccttagtagt
    12781 ggacacatcg cagttcctcc ttctttgttt tattatgaga gcgttgaatg gctggatgga
    12841 aacttaagtg ggatggacta ccttcgtctc ataaaaaaca tctggaagtc agacctaaac
    12901 ttgagagatg aaatcgccta ctgggaaatg gctgactata tcagtcttcc catccgcaag
    12961 tattccttag gcatgaagca acgcttggtg attgctatgt attttctcag tcaggccaaa
    13021 tgctggctca tggatgagat tacaaatggc ttagacgagt attatcgaca gaagtttttt
    13081 gataggctag cacaaatcga tagacaagaa cagctggttc ttttaagttc ccactataag
    13141 gaagagttgg ttgatatctg cgatagagta gtaaccattc atcaggggca gatagaagag
    13201 gtttagttta tgaaagatgt tagtctattt ttattgaaaa aagttttcaa aagtcgttta
    13261 aactggatta tcttactttt atttgcatct gtactcggtg ttacctttta tttaaatagt
    13321 cagactgcaa actcacacag cttggagagc aggttggaaa gtcgcattgc agccaacgag
    13381 agggctatca atgaaaatga agagaaactc tcccaaatgt ctgataccag ctcggaggaa
    13441 taccagtttg ctaaaaataa tttagacgtg caaaaaaatc ttttgacgcg aaagacagaa
    13501 attctgactt tactaaaaga agggcgctgg aaagaagcct actatctgca gtggcaagat
    13561 gaagagaaga attatgaatt tgtatcaaat gacccgactg ctagctctgg cttaaaaatg
    13621 ggggttgacc gcgaacggaa gatttaccaa gccctgtatc ccttgaacat aaaagcacat
    13681 actttggagt ttccgaccca cgggattgat cagattgtct ggattttaga ggttatcatc
    13741 ccaagcttgt ttgtggttgc gattattttt atgctaacac aactatttgc agaaagatat
    13801 caaaatcatc tggacacagc tcacttatat cctgtttcaa aagtgacatt tgcaatatcc
    13861 tctcttggag ttggagtggg atatgtaact gtgctgttta tcggaatctg tggcttttct
    13921 tttctagtgg gaagtctgat aagtggtttt ggacagttag attatcccta cccaatttat
    13981 agcttagtga atcaagaagt aactattggg aaaatacaag atgtattatt tcctggcttg
    14041 ctcttagctt tcttagcctt tatcgtcatt gtggaagttg tgtacttgat tgcttacttt
    14101 tttaagcaaa aaatgcctgt cctctttctt tcactcattg ggattgttgg tttattgttt
    14161 ggcatccaaa ccattcagcc tcttcaaagg attgcacatc tgattccctt tacttacttg
    14221 cgttcagtgg agattttatc tggaagatta cctaagcaaa ttgataatgt caatctaaat
    14281 tggagcatgg gaatggtctt acttccttgt ctgattatct ttttgctagt ggggattcta
    14341 tttattgaaa gctggggaag ttcacgtaaa aaggaagttt ttaataggtc ttagcattct
    14401 tataaggaag ttaaggaaaa actaacatag agagggaatc aacttgattc tctctttttg
    14461 attcgaaaac caaaccaaaa tacaaa
//
LOCUS       DAVKQL010000050        14073 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVKQL010000050 DAVKQL010000000
VERSION     DAVKQL010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14073
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.050"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            21..818
                     /locus_tag="VW561_001581"
     CDS             21..818
                     /locus_tag="VW561_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEU9943816.1"
                     /translation="MISSEFISKIEFACNKKEGLYSQSKFKYAIRSIFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLIHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            966..1088
                     /locus_tag="VW561_001582"
     CDS             966..1088
                     /locus_tag="VW561_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEU9943817.1"
                     /translation="MTCDFKFETLQLHAGQVVAPATKSRAVPIYQTTSFIFDDT"
     gene            1110..2150
                     /locus_tag="VW561_001583"
     CDS             1110..2150
                     /locus_tag="VW561_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HEU9943818.1"
                     /translation="MRKTGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILAIAH
                     AGDHVVAASTIYGGTFNLLKEPLPRYGITTTFFDIDNLEEVEAAIKDNTKLVLIETLG
                     NPLINIPDLEKLAEIAHKHQIPLVSDNTFATPYLINVFSHGVDIAIHSVTKFIGGHGT
                     TIGGIIVDSGRFDWTASGKFPQFVDEGPSCHNLSYTRDVGAAAFIIAVRVQLLRDTGA
                     ALSPFNAFLLLQRLETSSLRVERHVQNAETIVDFLVNHPKVEKVNYPKLADSPYHALA
                     EKYLPKGVGSIFTFHVKGGEEEARKVIDNLEIFSDLANAADAKSLVVHPATTTHGQLS
                     EKDLEAAGVTPN"
     gene            2321..3595
                     /locus_tag="VW561_001584"
     CDS             2321..3595
                     /locus_tag="VW561_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEU9943819.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMKQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHEALLAKDKEV
                     QLLENQLATLRLEHENQLQKTLSDLEKERDQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3605..4483
                     /gene="truB"
                     /locus_tag="VW561_001585"
     CDS             3605..4483
                     /gene="truB"
                     /locus_tag="VW561_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU9943820.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEIILGYSTTTEDASGEVVAETPVLSSLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            4724..5026
                     /locus_tag="VW561_001586"
     CDS             4724..5026
                     /locus_tag="VW561_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU9943821.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQS
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            5134..5772
                     /gene="udk"
                     /locus_tag="VW561_001587"
     CDS             5134..5772
                     /gene="udk"
                     /locus_tag="VW561_001587"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEU9943822.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            5900..7240
                     /locus_tag="VW561_001588"
     CDS             5900..7240
                     /locus_tag="VW561_001588"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU9943823.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESIVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            7218..7430
                     /locus_tag="VW561_001589"
     CDS             7218..7430
                     /locus_tag="VW561_001589"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEU9943824.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            7427..8302
                     /locus_tag="VW561_001590"
     CDS             7427..8302
                     /locus_tag="VW561_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU9943825.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVAIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            8295..9110
                     /locus_tag="VW561_001591"
     CDS             8295..9110
                     /locus_tag="VW561_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEU9943826.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVYGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKVHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            9103..9534
                     /locus_tag="VW561_001592"
     CDS             9103..9534
                     /locus_tag="VW561_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9943827.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            9541..11208
                     /gene="recN"
                     /locus_tag="VW561_001593"
     CDS             9541..11208
                     /gene="recN"
                     /locus_tag="VW561_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEU9943828.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            11210..11938
                     /locus_tag="VW561_001594"
     CDS             11210..11938
                     /locus_tag="VW561_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEU9943829.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEAADLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            12017..13840
                     /gene="lepA"
                     /locus_tag="VW561_001595"
     CDS             12017..13840
                     /gene="lepA"
                     /locus_tag="VW561_001595"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEU9943830.1"
                     /translation="MNLEELKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(13921..>14073)
                     /locus_tag="VW561_001596"
     CDS             complement(13921..>14073)
                     /locus_tag="VW561_001596"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943831.1"
                     /translation="KHSFEVVDETDEVSSKHGFEVVDETDEVSNHTYGKATLTWFEEI
                     FEEYKN"
BASE COUNT         4148 a         2576 c         3357 g         3992 t
ORIGIN      
        1 aaaagattta ggagtttatt atgatttctt cagaatttat ctcaaagatt gaatttgctt
       61 gcaataagaa agaaggtctt tatagtcaaa gcaaatttaa gtatgcgatt cgttcgatat
      121 tcgcaggtgc atttttaacc ttcagtactg ctgcaggtgc agttggggct gacttgatta
      181 ataaaattgc accaggtagt ggacgcttcc tctttccatt cgtttttgct tggggcttgg
      241 cctacattgt ttttttgaat gccgagttgg tcacttcaaa catgatgttc ttgactgctg
      301 gtagtttctt aaaaaaaatc tcttggagaa aaacagctga gattttacta tactgtacct
      361 tgttcaacct tatcggagcc ttgatagcag ggtggggctt tgctcattcg gcagcctatg
      421 cgaatctgat acacgatagt ttcatctcag gtgttgttga gatgaagtta ggccgctcaa
      481 atgaattggt cttgcttgag gcgattttgg caaatatttt cgtaaatatt gcgattctgt
      541 catttatttt ggtcaaagat ggtggtgcca aactttggct tgtgttgtca gctatttaca
      601 tgtttgtatt cttaacaaac gagcacattg cggcgaactt tgcttctttc gcgattgtga
      661 aattcagtgt tgctgcggat tcaattgcca acttcggtgt tggaaatatg cttcgccact
      721 ggggtgtgac tttcatcgga aactttatcg gaggaggcct cttgatgggt cttccatatg
      781 ccttcctcaa taaaaacgaa gatacttatg tagattaaga aaatgagcac gattgagtcg
      841 tgcttttttc attttcaaaa taaggtaata gctatttctt atatcaaaat atagaaaact
      901 gatatttgta aactataact caaggtgcta caatatcctt aataaaatga tatggaggtc
      961 accttatgac ttgtgatttt aaatttgaaa ctctacaact acatgctggt caagttgtgg
     1021 ctccagctac taagtctcgt gcagtgccga tttatcaaac aacatctttt atttttgatg
     1081 acacgtagga aggtgctgat ctgtttgcct tgaggaaaac agggaacatt tatactcgta
     1141 tcaccaatcc tacaacagct gcccttgaag gtggtgttga agcgctagca acagcatcag
     1201 gtatgactgc agtgacttat actattttgg cgattgccca tgctggtgac catgtagtgg
     1261 ctgcttcgac tatttacggt ggaaccttca atcttttgaa agaacccctt cctcgttatg
     1321 gtatcacaac aacctttttc gatattgata atttggagga agtagaagca gctatcaaag
     1381 acaataccaa gcttgtcttg attgaaacct tgggtaaccc cttgattaat attccagacc
     1441 tggaaaaact ggcagagatt gctcataaac atcaaatccc acttgtgtca gacaatactt
     1501 ttgcaacacc ttatttgatt aacgtcttct ctcatggcgt tgacattgcc attcactctg
     1561 tgactaagtt tatcggtggg catggtacaa ctattggagg aataattgtc gatagtggtc
     1621 gttttgactg gacggcttca gggaaattcc ctcaatttgt tgacgagggt ccaagctgcc
     1681 acaatttgag ctatactcgt gatgtgggtg cagcagcctt tattatagct gttcgagttc
     1741 aattgcttcg tgatacaggt gcagccttgt caccattcaa tgctttcctc ttgctacaaa
     1801 gacttgaaac ctcttcactt cgtgtggaac gccatgtaca aaatgctgag acaattgttg
     1861 attttcttgt caaccatcct aaggtagaaa aggtaaatta tccaaaactt gcagatagtc
     1921 cttatcatgc cttggctgag aaatacttgc caaaaggtgt cggttcaatc tttaccttcc
     1981 acgtcaaagg tggcgaggaa gaagcacgca aggtcattga taatttagaa atcttttctg
     2041 accttgcaaa cgcggcagat gctaaatcgc ttgttgtcca tccagcaaca accactcacg
     2101 gtcaattgtc agaaaaagac ctagaagcag caggtgtcac accaaactaa attcgtttgt
     2161 caatcggtct tgaaaatgta gaagatttga ttgaagactt gcgcttggcc ttggaaaaaa
     2221 tttaaagtaa aagaagataa acagtgggct tcgactcact gtttttgatt ttccctcagg
     2281 catgatataa tggttaaaga agtctagaaa gaggaacgat atgaacgaaa tcaaatgtcc
     2341 caactgtggg gaagtcttta cagtaaatga gagtcagtat gctgaacttt tgtcccaagt
     2401 gagaacggca gagtttgata aggaactaca cgataggatg aagcaggaac tggccttggc
     2461 tgagcaaaag gccatgaatg agcaacagac taaactggct cagaaggatc aagaaattgc
     2521 gcaattacag agtcagatcc aaaactttga tacagaaaaa gaattggcca agaaagaggt
     2581 tgaacagaca agccatgagg ctctcttggc taaggacaag gaagtacagc tcttagaaaa
     2641 tcagttggct accttgcgtt tggagcatga aaatcaacta caaaagaccc tttctgacct
     2701 agaaaaagaa cgggatcagg ttaaaaacca actacttttg caggaaaagg aaaatgaatt
     2761 atctttggct tctgttaagc aaaactacga agcccagctc aaggcagcta gtgaacaagt
     2821 cgagttttat aagaatttta aggctcaaca atctacaaaa gcgattgggg aaagcctaga
     2881 acagtatgca gagagtgagt ttaacaaggt tcgtagtttc gcctttccaa atgcttactt
     2941 tgagaaggat aacaaggtct cttcgcgtgg gtctaaaggg gactttatct tccgtgagtg
     3001 tgatgaaaat ggagttgaaa tcatttctat catgtttgag atgaaaaacg aagcggacgg
     3061 aacagagaag aagcacaaga atgcagattt ttacaaggaa ttggacaagg accgtcggga
     3121 gaagaactgt gagtatgccg ttttggtgac catgcttgag gctgataatg actactttaa
     3181 cacagggatt gttgacgtca gtcacgagta tgaaaaaatg tatgttgttc gtcctcaatt
     3241 ctttatccaa ttgattggtc tcttacgtaa tgcggcgcta aattccctaa aatacaagca
     3301 ggagttggcc ttggttcgcg agcaaaatat tgacattacg cattttgagg aagatttgga
     3361 tgcctttaag ctagcttttg ctaagaacta taattcagct tcgactaact ttggaaaagc
     3421 tattgatgaa atcgacaagg ccatcaaacg catggaagag gttaagaaat tcctgaccac
     3481 atctgaaaac caactccgtt tagctaacaa caaattggaa gatgtctctg ttaaaaaatt
     3541 gacccggaaa aatccaacaa tgaaagcgaa gttcgaagca ctgaaggggg agtagaaagc
     3601 aaaaatgaac ggtattatca acttaaaaaa agaagcagga atgacctcgc atgatgcggt
     3661 ttttaaactg cgtaagattt tgggaaccaa gaaaattggt catggtggaa ccttggatcc
     3721 ggatgtggtg ggtgttttgc cgattgcggt tggcaaggcg acacgcatgg tcgagtttat
     3781 gcaggacgag ggtaagatct atgaggggga aatcattctg ggctattcca cgacgactga
     3841 ggatgctagt ggggaagtgg tcgcagaaac ccctgttttg tcttcattgg atgaaaagct
     3901 tgttgatgaa gcgattgcta gcttgactgg gcctattact cagattcccc ctatgtattc
     3961 ggcagttaag gttaatggtc gcaagctcta tgagtatgcg cgtgctggtc aggaagtgga
     4021 gcgtccagaa cgtcaggtga ccatttatca atttgaacga acaagtccga tttcttatga
     4081 tggccaactt gcccgattca cttttcgtgt aaaatgcagt aaagggactt acatccgtac
     4141 tttgtcagtt gatttgggtg aaaagcttgg ttatgcggct catatgtccc atttgactcg
     4201 tactagtgct gctggcttac aattagaaga cgctcttgcc ttggaggaaa ttgctgaaaa
     4261 agtagaggct gggcaattag attttctcca tcctttagag attgggacag gtgaccttgt
     4321 caaagttttc ctaactccag aagaggctac agaagttcgc tttggtcgtt ttattgagct
     4381 agaccaaacg gacaaagaac tggctgcctt tgaagatgat aaattgttag ccattctaga
     4441 aaaacggggc aatctctata agccaaggaa ggtttttagc tagatcgttt aggaataaaa
     4501 atcgggtgat agataacaat tgcttgataa aaccccatac taatagtaga atggttttgg
     4561 gaattataat attccaattg ttgcgagttg taggtactca aataatctat atagaaattt
     4621 agaggtgtga aatgaagcaa tttaaaattc tttcagataa atatttagag tccattacag
     4681 gttctggtgg gaacttaggc ccaggatttg gtgtgataat tccatgatgc gaaatgagtt
     4741 tcgagaaagg gtggagcaac ttcttcaaca aaaagaaata aatgaaaata gtgagttgag
     4801 tcacctgttt cgtcttgcta tacaaaattt agacagaaat gaaaaatacc aatcggtcat
     4861 ggccaatttg agtcaagggt tgtcacttta cctcatgacg catcattacc aggcacctaa
     4921 gtctgtcatt gattttggtt tatggattgc caaagctcct agccaggaaa gagggagact
     4981 ggctttcttg cagatgcttg ctcaaaccct acagggcttt cgttaaaact gtcgaacatc
     5041 agagggaaga ctctggtgtt ttttgctgat gagactataa attttgtaaa aaatgtgata
     5101 gaatagacga cggataaaat aacggaggat agcatgcaaa atagaccaat cattattgga
     5161 gtgacaggtg gttctggtgg tggtaaaacc agtgtttcaa gagccatttt atcgcatttt
     5221 cctgatgaaa agatttccat gattgagcat gattcatact acaaggatca gtctcatttg
     5281 acctttgaag agcgtgtcaa aaccaactat gaccatcctt ttgcctttga tacagacttg
     5341 atgatcgagc agattaagga attgttggca gggcgtccgg tggacatccc gacctacgac
     5401 tatacagagc atacacggag tagcaagacc tatcgtcagg agcctcaaga tgtctttatc
     5461 gttgagggca ttttggtctt ggaggacaag cgtctgcgcg atttgatgga tatcaagatt
     5521 tttgtggata cggatgacga tgtgcgcatt attcgtcgta tcaagcgtga tatggaggag
     5581 cgtggccgta gccttgatag cgttattaac cagtacttag gtgtggtcaa accaatgtac
     5641 caccagttta tcgagtcaac taagcgttat gctgatatcg tcattcctga aggggttagc
     5701 aataccgtgg ctatcgacct gttgacgacc aagattgcaa agattttgga agaagctcga
     5761 aacagcaaat aatcacatgt ggaggccttg cctccttttt ctatttttcc ctcataatgg
     5821 tacataaatg gagattttta ggcatatttt tggtataata atacccataa aagagcaagt
     5881 aaatgaatag taggtggaga tggaaaagta tttatcggta acaactttga ccaagtatct
     5941 gaaaatgaaa ttcgataaag acccatactt ggaacgggtc tatttaactg gtcaagtttc
     6001 caactttcgt aaacgaccta ctcaccaata tttctcccta aaggatgacc atgcagttat
     6061 tcaagcgacc atctggtctg ggatttatca gaaattaggg tttgacctgg aagaaggaat
     6121 gaagatcaat gtgattgggc gtgtacaggt ctatgaacca agtggtagct actccatcat
     6181 cattgaaaaa gctgagcctg atggggttgg ggcgcttgcg attcagtttg aacaacttaa
     6241 gaaaaaattg acagaagaag gcctgtttca agaacgcttc aagcaagctc taccccaatt
     6301 ttctaagaga attggggtag taaccagccg tagtggagcc gttattcgag atattatcac
     6361 gaccgtcagc aggcgattcc caggtgttga catccttctt tatccgacca aggttcaagg
     6421 tgaaggggct gcggaggaaa ttgctagaaa tattgcgcgt gctaatcaac gggatgattt
     6481 ggatttgctc attattggtc gtggaggtgg ttctattgag gatctctggg cctttaacga
     6541 agaaattgtg gtacgagcta tttttgaatc tcgtttgcca gttatctcta gtgtggggca
     6601 tgagacggat gtgaccttgg cagattttgt ggcagatcga cgcgctgcaa cgccaacagc
     6661 ggcggctgaa ctggcaacac ctgtgaccaa gttggatgta ttagctcatt tgcaaaatca
     6721 ggaaaaacgg atggcaacgg cagtccgaaa tgttctatct aagaaacaag aggctttgaa
     6781 aaaatgcagt cagtctgtta tctttagaca acctgagcgc ttgtatgacg gttatttgca
     6841 acgcttggac caactgcaac tgcgtttgaa acaaagtttg cgaactcgga tttctgataa
     6901 caagcaatta gttcaagcaa gaactcatca attagtacaa ttatcacctg ttaccaaaat
     6961 ccaacgctat caagaccgtt taggacagtt ggacaagctc ttaggtagcc aaatggcgtt
     7021 agtttatgac gccaaggttg ctgaggccaa gcgactttcg gaagctttgc tcatgttgga
     7081 tactagccga atcgtggcgc gtggttatgc tattgtcaaa aaagaagagt ccattgtaga
     7141 ttcggttgag agtttgaaga aaaaagacca agtaacgctt ttgatgcgag atggtcaagt
     7201 agaattagag gttaaagatg tcaaaacaaa agaaatttga ggaaaatcta gcagaactgg
     7261 aaaccattgt ccaaagtttg gaaaatggtg aaattgctct ggaagatgcg attactgcct
     7321 ttcaaaaggg catggtcttg tcaaaagagc tccaagctac gctggacaag gctgaaaaga
     7381 ccttggtcaa ggtcatgcaa gaagacggaa cagaaagtga ttttgaatga aaaagcaaga
     7441 aaaattagct cttgtcgagt cggctttgga agatttttat ggagaccagc agtttgcctc
     7501 tagtttacgg gagtctgttc tctattctat tcatgctggt ggcaagcgta ttcggccttt
     7561 tctcttgtta gaagttctgg aagccttgca ggttgccatc aaacctgctc acgcgcaggt
     7621 agctactgcc ttggagatga ttcatacagg gagcttgatt cacgatgacc ttcctgctat
     7681 ggatgatgac gattatcgaa gagggcggtt aaccaatcac aagaaattcg gtgaagctat
     7741 ggccattttg gctggagatg ccttattctt agacccatat gccttgattg cgcaggcaga
     7801 tttgccaagt cagatcaagg tggacttgat tgccaactta tcccttgctt caggtagtct
     7861 gggtatggtg gcagggcaag ttttggatat ggagggcgaa caccagcact tgtctctgga
     7921 agaacttcag actattcatg ccaataagac tgggaagtta ctagcctatc ccttccaagc
     7981 ggcagctatt atagctgaat tgtcacctga aatgcaggtg aagctgaaaa ctgtgggtga
     8041 attgattgga cttgcttttc aagtcagaga tgatgtactg gatgtgacag ctagttttga
     8101 ggaaatcggc aagacacctc aaaaggatct gcaggcagaa aaatcaacct atcctgcctt
     8161 gttgggcttg gaagagtcca ttgccttttg taaccaaacc ctggatcaag ctaatgaaaa
     8221 attagaagaa attgcccagc aacttccctt tgaaacagaa tcgattgtaa gtgtagtaga
     8281 aagtttgaga atcaatggct aaggaaagag tggatgtact agcttataaa caggggttgt
     8341 ttgaaacgag agagcaggcc aagcgaggtg tgatggctgg cctagtcgta gcagtcctta
     8401 atggagaacg gtttgacaag ccaggagaga aaattccaga tgacaccgaa ttaaaactca
     8461 agggggagaa actcaagtat gtcagccgtg gtggtttgaa actggaaaag gccttgcagg
     8521 tctttgattt gtcggtgtat ggcgcgacta cgattgatat cggggcctct actggaggtt
     8581 ttaccgatgt catgctacag aatagtgcca agttggtctt tgcagtcgat gttggtacca
     8641 atcagttggc ttggaaatta cgccaagacc cacgagttgt cagcatggag cagttcaatt
     8701 tccgctatgc tgaaaagact gatttcgagc aggagccgag ctttgccagt attgatgtga
     8761 gtttcatttc ccttagtctg attttgccag ccttgcaccg tgtcttggct gatcaaggtc
     8821 aggtggtagc acttgtcaaa cctcagtttg aggcaggacg tgagcagatt gggaaaaatg
     8881 gaattattcg agatgctaag gttcatcaga atgtccttga atctgtaaca gctatggcag
     8941 tagaggcagg tttttcagtc cttggcttgg acttttctcc catccaaggt ggacatggaa
     9001 atattgaatt tttagcgtat ttgaaaaaag aaaagtcagc aagcaatcag attcttgctg
     9061 agattaaaga agcagtagag agggcgcata gtcaatttaa aaatgaataa aaaagagaga
     9121 cttgaaaaaa ttagaagatt ggtgacagat tatcaaatcg gcacgcaaga agaaattgta
     9181 gaacatttga aagaagcagg tatcactgcc actcaggcga cggtatcccg agatatcaaa
     9241 gagttaggta ttgtcaaaat tcctttgaga gacaacacct atgtctacga attgccaaaa
     9301 tcaatcgtaa aaagtctgca actggctgaa gacaatatcg aatcggctga attgatggat
     9361 aagatgatca atctccaagt tattccagga aatacggctt ttgtaaaagc tcagttaatc
     9421 gagacttttg cagacaagat ttttagctgt ttgactgatg atagctcgat tttagtcatt
     9481 gccagaagtg gaagtctagc agaggaaatc tttgaacaag taaaaaattg gtaggtctat
     9541 atgttacttg aaatttcgat aaaaaacttt gccattattg aggctatttc cctcaatttt
     9601 gaaaagggga tgactgtcct gactggtgaa acgggtgcag ggaagtcgat tatcattgat
     9661 gccatgaata tgatgttggg agctcgtgcg acgacagatg ttattcgtca tggtgcacca
     9721 aaggcagaga ttgaggggct tttctcagtt gagaatagtc gtcttttaca ggaaattttt
     9781 gatgagcaag gtttagaatt aggtgatgaa attatcatcc gtcgggaaat cttgcaaaat
     9841 ggtcgtagta tcagccgtgt aaatggtcag atggttaatc tgtctgtttt gcgagctatt
     9901 gggcaacatc ttgtagatat tcatggtcag catgaccatg aggagttaat gcgtccccaa
     9961 ctgcatatcc agatgttgga tgaatttggt gatgccgctt tttgggactt gaaagaaacc
    10021 tatcaaacga gttttgatgc ctatcggaaa atgcgcaagc aggttctgga agtcaagaaa
    10081 aaccaacagg aacacaaggc acgtatcgaa atgttggaat ttcaaatggc agagattgag
    10141 gcagcaaact tgcaggctgg agaagacttg gctctcaatc aagagcgaga taaactcctc
    10201 aaccacaaaa atattgcgga tacactgacc aatgcctaca gtatgttgga caatgaagat
    10261 ttttcaagtc tggccaatgt tcgttcagct atgaatgaca tggaaagtgt cgaagagtat
    10321 gatcctgaat accgtgaaat ttcaagctct ctgtctgaga cctactatgt tttagaagac
    10381 attagcaaac gtttggaagc tatcattgag gaccttgatt ttgatggcaa tcgcctgatg
    10441 caggttgaga atcgtttgga cctccttcat actattactc gtaagtatgg tgggactgtt
    10501 gatgatgttt tgctttattt tgccaagatt acggaagaat acaatctctt gacaggcaat
    10561 aatctttcgt ctgaggacat ggaagcagag cttaagaagt tggaagtcaa tcttgtcaat
    10621 ttggcaggtc aacttgcttc tgctcgtcat aatttggctc agcaactcga agctgagatt
    10681 aaacaagaac tgcaagatct ttatatggaa aaagcccagt ttcaggttcg ttttagtaag
    10741 ggaaaattca gtcgtgaggg aaatgaaatg gttgagtttt atatttcaac caaccctgga
    10801 gaagacttta aacccttggt taaggttgct tctggagggg aattatctcg tctcatgtta
    10861 gccattaagt ctgccttttc acgtaaagaa ggcaagacta gcattgtctt tgatgaggtg
    10921 gatacgggag tttcaggtcg tgttgctcaa gctattgctc agaaaattca taaaattggt
    10981 cagcatggtc aggttttggc tatctcccat ttgccacaag taattgcgat tgcagattat
    11041 caattcttta ttgagaagat tagtaatgac cattcaacgg tttcgactgt tcgtctcttg
    11101 acggtcgaag agcgagtgga ggaagttgcc aagatgttgg caggtgatga tgtgacagaa
    11161 gcagccctga cgcaagccag agaattgttg agaaacaggg agaaataaga tgacagatta
    11221 ttatgtaatt ggagatgttc acggaaaagc tgggatgctg gaagaccttc ttaaaacctg
    11281 ggatggtcag acccagttgc tctttctagg tgatttgatt gacagaggtg aggatagtca
    11341 ccgtgttctt gaaatggtca aggacttggt ggacaatcaa ggggctatct gtttgtctgg
    11401 aaaccacgag tatatgtttt tgacttggct agatgaccca gaagaaagtt atgaccatta
    11461 tcgtcgcaat ggtggagata caaccattaa ctctatccta ggtcgtccct tggatgcacc
    11521 agttgatgga gtagaagatg ccaagcgggt tgccgctgaa gcggcagact tggtcgaatt
    11581 cattcgtcaa atgccatttg tagtagagac agacaagtat atctttgttc acgcaggtat
    11641 tgatttgact ttggacgact ggcatgaaac aacagattat aaaaaagtat ggcttagaaa
    11701 accattccac ggagctgaaa atcatactgg aaaaaccatt gtcttcggtc atacaccggt
    11761 ctatggtttg ttacagcaag aacgcggtac atctgagctt tggactacag aagatggcaa
    11821 gattggaatg gatggaggag ctgtttatgg tggtgtcctt cacgggattg tctttacaga
    11881 ccaaggaatg acagaacacc actttatcga aaatgacggc tttgtcgctg aagattagta
    11941 ctcctagcag ggtatggtct tgtcaaaata tcaaaaacaa tttataataa atagataccc
    12001 tgaaaggaag agaatcatga acttagaaga attgaaaaaa cgacaggaga agatccgtaa
    12061 cttctctatt atcgcccata ttgaccacgg aaaatcaact ctagcagacc gcattttgga
    12121 aaaaacagag acggtttcaa gtcgtgaaat gcaggcccag cttttggata gcatggatct
    12181 agagcgggaa cgtggaatta ctattaagtt gaatgccatc gagttgaatt acactgcaaa
    12241 agatggggaa acttatattt tccacttgat tgacacacca gggcacgttg actttaccta
    12301 tgaagtttca cgttcgctag ctgcctgtga gggtgctatt ttggtggtcg atgcggctca
    12361 aggaattgag gctcaaactc ttgccaacgt ttatctggct ttggataatg atttggaaat
    12421 catgcctatc attaataaaa ttgacctgcc ggctgcagat ccggagcgcg tgcgtacaga
    12481 gattgaagat gtgattggtt tggatgccag tgaagcagtt ttggcttctg ccaaggctgg
    12541 tattgggatt gaagaaatcc tcgagcaaat tgtagaaaaa gtaccagcac caacgggtga
    12601 tgtgacggcg ccacttaagg ccttgatttt cgactctgtt tacgatgctt accgtggggt
    12661 tatcctccaa gtacgtgtca tggacggagt ggtcaaacct ggtgataaga ttcagctcat
    12721 gagcaatagt aagacctttg atgtggccga agtcggtatt tttacaccca aagcggttgg
    12781 tcgtgatttc cttgcgactg gtgatgttgg ttacattgcg gcgtctatca agacggttca
    12841 ggatactcgt gtgggtgata ccgttacctt ggcaaccaat cctgcggcag aaccattaca
    12901 tggttataag cagatgaatc ctatggtctt tgcgggtctc tacccaatcg aatcaaacaa
    12961 gtacaatgac ctgcgtgaag cgcttgaaaa attgcaactg aatgatgcta gtcttcagtt
    13021 tgaaccagaa acatctcagg cacttggatt tggtttccgt tgtggatttc ttggacttct
    13081 ccatatggat gttatccagg aacgtttaga gcgtgagttc aatattgacc tcatcatgac
    13141 agctccgtct gttatttaca aagttaattt gaccgacggt gagtctatgg atgtgtctaa
    13201 cccatctgag tttccagacc caactaagat tgcgaccatt gaagagcctt atgtcaaggc
    13261 gcaaatcatg gtaccacagg agttcgtcgg agcagtaatg gagctagctc agcgtaagcg
    13321 tggggacttt gtgactatgg actatattga tgataaccgt gtcaatgtta tctatcaaat
    13381 tcctcttgct gaaattgtct ttgacttctt tgataaactt aagtcttcga cacgtggtta
    13441 tgcaagcttt gactacgaat tgtcagaata tcgcccatct aagctggtga aaatggatat
    13501 ccttctcaat ggagataagg tggatgccct cagctttatc gttcacaagg actttgccta
    13561 cgaacgtggg aaactcatcg ttgataaact caagaaaatc atccctcgcc aacaatttga
    13621 agttccaatc caagcggcta ttggacacaa gattgtcgct cgtactgata tcaaggccct
    13681 tcgtaagaac gtacttgcta aatgttatgg tggtgacgtt tctcgtaagc gtaaactcct
    13741 tgaaaaacaa aaagctggta agaaacgcat gaaatccatc ggatcagttg aagttccgca
    13801 agaagccttc ctcagcgtct tgagtatgga tgaagaataa gttatcagaa atgcctttcc
    13861 ttgttcacag gaaatttata taagaatcaa tagtaaagca ggcttagatg gtctgctttt
    13921 ttagttttta tactcttcga aaatctcttc aaaccacgtc agcgtcgcct taccgtatgt
    13981 atggttactg acttcgtcag tttcatctac aacctcaaaa ccatgttttg agctgacttc
    14041 gtcagtttca tctacaacct caaaactgtg ttt
//
LOCUS       DAVKQL010000051        13524 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVKQL010000051 DAVKQL010000000
VERSION     DAVKQL010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13524
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.051"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(<1..1448)
                     /gene="pfbA"
                     /locus_tag="VW561_001597"
     CDS             complement(<1..1448)
                     /gene="pfbA"
                     /locus_tag="VW561_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEU9943832.1"
                     /translation="MKYFVPNEVFSIRKLKVGTCSVLLAISILGSQGILSDEVVTSSS
                     PMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNSISSNGTIRSNS
                     QLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAVGDGIHDDRQAI
                     QDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILNGINIKNHPSIV
                     FMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLINSSGAFAIGNS
                     NNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQIISKESIQIEP
                     LTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTLSTQNPSNIKIL
                     NNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVNAYSYKNTKDLL
                     DLNKQVVIAENIFNIADPKTKAIRVAKDSAEY"
     gene            complement(1761..1991)
                     /locus_tag="VW561_001598"
     CDS             complement(1761..1991)
                     /locus_tag="VW561_001598"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943833.1"
                     /translation="MSDIKVNIPGECLYDKVFVLSFIIYNISINLNIVNGIFYIQAKK
                     DSITFEWKAKEQTRKLAIDSSKPCFEVVDIVK"
     gene            complement(2611..3837)
                     /locus_tag="VW561_001599"
     CDS             complement(2611..3837)
                     /locus_tag="VW561_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEU9943834.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVEFAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(3880..4572)
                     /locus_tag="VW561_001600"
     CDS             complement(3880..4572)
                     /locus_tag="VW561_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU9943835.1"
                     /translation="MLKWEDLPMEMKSSEVESYYQLVSKRKGSLIFKRCLDWFLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            complement(4745..6496)
                     /locus_tag="VW561_001601"
     CDS             complement(4745..6496)
                     /locus_tag="VW561_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9943836.1"
                     /translation="MQNKQEQWTVLKRLMSYLKPYGLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLAYANRSVALDALFLRPAMSLLKLLGYAVLMTYFGYRGLSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALIDERTYEPLQENGQA
                     KVKEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLALGG
                     TYHKMYSLQAGAMTYTL"
     gene            complement(6486..8231)
                     /locus_tag="VW561_001602"
     CDS             complement(6486..8231)
                     /locus_tag="VW561_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9943837.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALILVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLVVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADLILVLQNGQIIERGRHEDLLALDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(8437..8814)
                     /locus_tag="VW561_001603"
     CDS             complement(8437..8814)
                     /locus_tag="VW561_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEU9943838.1"
                     /translation="MKRLIDGKPTLLIKNGNIDPEACRSVGLSASDVSLKLRSQGIFQ
                     MKQVKRAVQEQNGQLIVVQMGDENPKYPVVTDGVIQVDVLESIGRSEEWLLDNLSKQG
                     HDNVANIFIAEYDKGAVTVVTYE"
     gene            complement(8886..9342)
                     /locus_tag="VW561_001604"
                     /pseudo
     CDS             complement(8886..9342)
                     /locus_tag="VW561_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            9476..10303
                     /gene="xth"
                     /locus_tag="VW561_001605"
     CDS             9476..10303
                     /gene="xth"
                     /locus_tag="VW561_001605"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU9943839.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDAKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            10315..10413
                     /locus_tag="VW561_001606"
     CDS             10315..10413
                     /locus_tag="VW561_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEU9943840.1"
                     /translation="MDYNAVIPELLVSNIEQSRSFYCGLLGFRIEY"
     gene            10458..10697
                     /locus_tag="VW561_001607"
     CDS             10458..10697
                     /locus_tag="VW561_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEU9943841.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVPKLYQKVMEANYPI
                     YRLLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     regulatory      11080..11175
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            11575..12156
                     /locus_tag="VW561_001608"
     CDS             11575..12156
                     /locus_tag="VW561_001608"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEU9943842.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMQEIGKVFAE
                     KFAATGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            12156..13418
                     /locus_tag="VW561_001609"
     CDS             12156..13418
                     /locus_tag="VW561_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEU9943843.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LSAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLFAIVLNAVLNHKKK"
BASE COUNT         4010 a         2909 c         2620 g         3985 t
ORIGIN      
        1 aaatattctg cactatcttt tgcaactcgt atcgcttttg ttttaggatc ggcaatatta
       61 aatatatttt cggcgataac cacctgttta tttaaatcta ataggtcttt agtgttttta
      121 tagctataag catttactaa agctgctccg ctttctcgat aatgtacact ctctccttta
      181 actttcttat caaagcgatt tcctttgatt aatacatcag tgaatcctgt aaaacgtaca
      241 cctgcataca tcatgttatc aaaatgatta tttaaaattt taatattaga ggggttctgt
      301 gtcgacaatg tttgatagtg tgtgccaatt gctgttacta attccccaga tttatcactt
      361 ttgccaaaat aggaattttg aatagtcaca ttttcagatt ttttcccatc atcattcaag
      421 gcataaggaa aaccttttct agttaatggt tcaatctgaa tgctctcctt acttatgatt
      481 tgcccatcct tcatcgtttt gggtaaggct tgcccaagaa aacgagaatt atcaactaat
      541 acatttttcg aacctgcaat ttgaatagca tgcccttgat aactatcctt gaatgttaca
      601 ttttttatag ttacgttatt tgaattccca atagcaaatg cacctgaaga atttataagt
      661 ggtagatttt ttgctttagt tccttcttca ttcaaagcac cgttcatatc aatcgtacca
      721 ccagaataac taatatcttc tgttgggccc cattctactt gcgcaccatc atccgtaaat
      781 aaacctgtca taaaaacaat ggaagggtga ttcttaatat ttataccatt tagaattgta
      841 gctttctcat tcaattctaa gtgtgtatga ctttttaaaa aaacaatttc ttttactaaa
      901 taagttcctt caggaaaata tacatttcct ccacctagcc cttgagctgc agcatctatt
      961 gcatcttgaa ttgcttgtcg atcatcatga atcccatcac ctaccgcacc ataatctttt
     1021 acacttaaag cagtatcttc aaattctttt ttgataattc tgtcacttat aacatcttcc
     1081 ttataactct tattttcatt agtagatgtt actgtagatt caactgttct gttgtctaat
     1141 tgtgaattgg aacgaatagt accattagag ctgatactat taacacttaa cgaattatct
     1201 aaaccattag tggttgaatt agaggcaatc atttcagcag aacgattctg attaacagtt
     1261 ggtgaattat ctaaatcatt agtaattgca ttagaagact cttttgtagc catcggtgaa
     1321 gaactagtaa caacttcatc cgataaaata ccttggcttc ccaaaattga aattgccaat
     1381 agtaccgagc aagtccccac ctttaattta cgaatactga atacctcatt aggaacaaaa
     1441 tatttcatca gaacctccaa tttttttact atttttaaca attgattatt acttttctta
     1501 aaatagatag aatgacaccg ctgtcattat ctttatcata tattttccaa attgaattgt
     1561 caaggaataa cacaagaaaa tattaaaatt tatactcttc ggaaatctct tcaaactagg
     1621 tcagtgccgc cttgacctaa gtctaggtta ctgacttggt cagttctata gtagactgaa
     1681 actaaaatag tacacctcta cttctaaaac attgttagaa atcgatttga ctgtcccgat
     1741 caatttgtcc tattcttatt tcattttact atatctacaa cctcaaagca gggttttgag
     1801 ctatctatgg ccaacttcct agtttgctct ttagccttcc attcaaaagt gatgctatct
     1861 ttcttagctt gaatataaaa tatcccattt actatattta aatttattga tatgttgtaa
     1921 attataaatg aaagaacaaa taccttgtca tacaagcatt cgccagggat atttacctta
     1981 atatcgctca tatggcggat tgaaactaaa acactacaat atatttctta aatagacctt
     2041 ctataaaagc tagatttaac ctcgataaaa atagatgcag tgcttctaaa atactggaat
     2101 agggcatata ggtaatcgta gacaagtgtt ttatagattg tctcgcagga gatctaatga
     2161 tgtatagcaa gcatcactaa acgtatagaa tatttgtgtt attataatat caatatgcta
     2221 taaattctat catgcaaaag aatgactaga acatgaaaga caggcacaaa actttgaaca
     2281 atattctgaa aacaaaaaaa gacgcgagtt tccacaatct cactcatgga aactcacgtc
     2341 tttcaaaaaa tagggtgaat tgaaaactag aacaagacac tagattttct aaatgatgtc
     2401 tagtaaggat tctaaatgct acaccaaatt gctcacatct tatttcaaac cacgggactc
     2461 ccacaagaaa ggaaaatcct ataaaaattc tctctctgag gagaaagagg cctcaactac
     2521 aggtgtaaac tattatcaat tgaaaacaac agtttttaca ctcaattaga ctttttaggg
     2581 acaggatcac tttcgcttga ctgtagtttg tcattttttt gataaagtta gcactttttc
     2641 agaaactgtt ttgaaagtct caatgatata gtctacttct tcatcgctta atttagtatg
     2701 aagagggagg gtaatttcat tctcaaagaa ggcataggcc ttaggatagt tcgtcatatc
     2761 aaatccaaga ttcttatagg ctgtcaagag aggaagcggt ttgtagtgaa cattacttgc
     2821 aattcctgct ttagccaatt cttggatgat gaggttgcgt tcttctaggc ttgctccttc
     2881 tacacgggtg atgtagaggt ggcgtgaaga ttcgacagtt tcagtcttgt gtgccaaagg
     2941 atggatgcga gaacctgcaa aaccactatc atagcggtcc acaatgtcct tacggcgttg
     3001 caacaaactt ggatagcggt ccaattgtac caaaccaagt gaagccatga tatcggtcat
     3061 gttgcactta taggctggtg taacgatatc gtattcccat gaccccagtt gcatcttggc
     3121 aagagcatcc ttagtttgcc cgtgaaggga aaggatttgg aattccttgt acatctcttc
     3181 gtcatcaatc actggattgg ctttccaagt cgcacttcca ccttctgccg ttgtaaagtt
     3241 cttaacggca tggaatgaga aggaagtaaa atcagcgata gaaccagaag gttgtccttt
     3301 ataagtagat cccaaagcgt gggcactatc agagacaatg acaatacggt taaaggcctt
     3361 ttgccacttg cttgaagcgg taaagaagtc acgttttttc tccacgactt ggaacaaacg
     3421 gtcataatcg caaacaatcc ctgcgaactc tactgggata atcaccttag ttttctcagt
     3481 gatagcttgc tcaagcaggt catagtccat ctcaaacgta tctgcttgga tatccaccat
     3541 gacaggggtt gctcccacgt gcgtaatgac actacatgaa gccgtatagg tcatggctgg
     3601 aacgatgact tcatcaccag gtcccacttc caaaacgcgt aaaatcaact ccagagcggc
     3661 tgtcgcagag ttgagacaaa cagtcttagg tgtctgtgtg taaagagaca agcggcgctc
     3721 cagttctttt gttttaggac ctgttgtgat ccaaccagaa cgcagggtat ccgctacttc
     3781 agcaatttct gcttctgtga tatcaggcgg tgaaaatgga atattgtaat ttggcattga
     3841 tttgctcctt ttatctgtac tcattttctt atgactactt tattttagta cctcaaacac
     3901 ggtttgaaac atgattttga tgtccccaaa gaaactaaac tctcggagat aggcgaggtt
     3961 atagcgcatc ttttcaggaa gaacatgctc cacataggcc tgatcaactg acagaccttt
     4021 ctccgtcatt tgactgatga tggtgtcctc atccttgtag ttgatgctgg ctggagaggt
     4081 aattcctgct tgcaagagca aggttgccat catttcaggg ctatactgct ctgtataacg
     4141 tggcacttca ggtcgtgtac cgacaaagga catctcacct ttaaggacat tgaccaactg
     4201 aggcagttcg tccaaacgga cacgtcggat gaaatttcca accttggtaa tacggctatc
     4261 gttagcagaa gtcaccagac ttcctttttt atccgcatcc gtcaccatgg tacggaactt
     4321 ccaaatcttg aaccgacggt tgtactgggt cacacgctct tgcttgtaaa tcactggccc
     4381 cttactatcc aacttgatcc aaatgctcaa gatgagaaag atgggagagg ttagaaccag
     4441 taagaccaag gccaaaaacc agtccaagca acgcttgaaa atcagcgaac ccttcctttt
     4501 agagacaagc tggtagtaag actcaacctc gcttgatttc atttccatag gcaagtcttc
     4561 ccatttcagc atgctgttct cctttgtttt tattatacta tttgtcaaca tttttcatta
     4621 taccacaaaa tggaaaaggc ggtgaaagaa atctgtaatt tgaaggattt ccttctagta
     4681 ctcaatgaaa atcaaagtac agctttgaga ttgcagataa agctgacatg gtttaaagag
     4741 attttcaaag agtataggtc atggcccctg cctgcaaact atacatcttg tgataggttc
     4801 ctcccagagc caagagttcc tcatgggttc cactctcgat aatgcgtccc ttatccaaga
     4861 catagatgca gttggcgtct tgaatagtag aaaggcggtg agcgatagca atagttgttc
     4921 ggccctgtct catcttcgcc agagaagctt gaaccaagct ttctgtttca gagtcaatat
     4981 tggctgtcgc ttcatccaaa atcaggattt taggctggct ggcgactgtt ctagcaaagg
     5041 caagaagctg gcgctgccca gtagagaagc tcgaaccacg ctcggaaaca ggggagtcgt
     5101 acccctgagg aagttcttga ataaaggaat ctgcatccac aaaggctgcc gcagcctgaa
     5161 cctgctcatc actgatttct tggtacatgg cgatattgga cttaatagtt ccatgataga
     5221 ggaagggttc ctgtaagacc aaaccgatgt tttttctcag ctcttcttga ctgaaatccc
     5281 tgatatccac atcatccaag agaactctcc ctgactggaa ttcataaaag cgcatgagga
     5341 cattgataat agacgatttc cctgaacctg tatgacctac aaaggcaatg gtttcaccct
     5401 tattaacaga gaaagaaatg tcatccagaa tcggatgttt accgtcatac gagaaacaca
     5461 catgttcaaa acggatattg ccttctttga ctttggcttg cccattttct tgaaggggct
     5521 cataggtcct ctcatcgatt aaggcaaaga cacggcctgc agaaaccata gacgtttgca
     5581 gtgttgaaaa gttttgcgtc acctcaatca aagggtcaaa gaggcggttg atgtactgga
     5641 taaaagcata catggtcccg gccgttatcc cgatagaaag accacgatag ccaaaatagg
     5701 tcatcaagac agcataacct agaagtttca acaaactcat ggcaggtctc aaaaagaggg
     5761 catccaaggc tacagaacgg ttggcatagg ccaagtgctc ttggttgatt tcatcaaatt
     5821 ctgcctgcag gcgcttctct tgattaaaag cctgaataat cctgattccc tcgatattct
     5881 ctgccagctt actattgata tctgacaaga gacttctggt tttctcgatg attttcactg
     5941 acttttttcg atagagattg accaaaagga aaatcaaggg gagaaagagc aagactaatg
     6001 ctgtcaaacg aaaatccagc accaacatgg tataaagggt tgtcagaaag ataaaaactg
     6061 ctgagataaa gctggataaa atcccagaaa acatatcact aatcgtctcg gtatcgttgg
     6121 tcaaacgaga aacgatagaa cctgctggcg tcttgtcaaa gtaagacatg cccagtttct
     6181 ccatattggc aaaggcatcc cgacgaatat ccctaacaat actgtaagac acgcgcgcaa
     6241 agagaagatt gccgacatac tgaactacag tttgtaggat gtagagacca tagtagacca
     6301 gcaaaacggt cacggctagt tggttaagat tgctgagata ctggtcgata aagtgggaag
     6361 ccacaagggg aatgacactt ttaatgaccg tcgtcgctag gagaaaactg agtgccaaaa
     6421 aggtaaggag tccataaggc ttgagatagg acatcaagcg cttcaataca gtccattgtt
     6481 cttgtttatt ctgcatcttc ttctcccttc atttccagct gctgagactg gtaggtttgg
     6541 gcataccagc catccaaagc tagcaagtct tcgtgcctgc ctcgttcgat aatttgacca
     6601 ttttgcagga ctaaaatcaa atctgcatgg acaactgcac tcaggcgatg ggcagtgata
     6661 atggttgtct tgtcctttcg cgtctccttg aggttgtcga taatcgcata ctctgtcttg
     6721 gcatctacgg cggataagga atcatccaaa atcaagatat cagggtctaa aatcatagcc
     6781 cgactcatag ccaaccgttg cttttgacca ccagaaagac tgactccttt ttcaccaatc
     6841 agcgtatcaa atccttgagg catgtctaca atatcttggt aaactcgggc tagcttagta
     6901 gcttcctcga ccgctgaaag gggcaagtta ggattgccaa agcggatatt gtctaggatt
     6961 gaagtcgcaa aaagaaactg gtcctgagga acatagccca tgagactgcg aaggtctgtc
     7021 agacgatagt cccgaatatc gtgaccgttt agataaatgg cgcccttatc cacatcgtat
     7081 tcacgcaaga ggagcttgat taaggacgtt ttcccagagc ctgtctgccc aaccaagccc
     7141 agtgtttgcc cttttgccaa actaaagtga atatccgtca gtgtttcctc attttcaaaa
     7201 gcaaagctgt caatggcata ctccaaacgc ccattttcaa taccatccag aggaaactcc
     7261 gggtcttgta caggagattc ctgagacaaa agattttcaa tccgctggta agaaaccttc
     7321 cctcgctgag tagtattaaa gaggaagccg atggccataa gaggccagac cagcatatcc
     7381 aaatagctga taaaggtgac tagattccca actgtaatct gcccttcctg aaccaccaag
     7441 gagccaacca aaagcgttaa aacataggac gaaccaacaa acaagagaac catagggtca
     7501 aagagactat catatttcat ggtttgcagg ttcttttgga aggttaattc attgactgcc
     7561 tgaaaagact tcaactcgtc tgcctgataa ccgaaagact tggtcacttt gatacctgat
     7621 acggactcct gtaccttgtt attgagttca gaaaaagcag cttgggattc gccaaaggcc
     7681 ttatgagtct ttctccctag gcgactagtc gcataggcca tgaaaggtag ggggagaatg
     7741 gcaacaagag tcatttgcca tgagatgcta aagagcatgg tcaacaaagt caccagagcc
     7801 gtgatagagg catccaccgc agacatgaca ccgccacctg ctaaacgagt caaggcattg
     7861 atatcattgg ttgcgtgtgc catcagatca cccgtccgat aggtttgata aaaggctgac
     7921 gacatttttg tgaaatgctt aaacaagcga gaccgcatga tctgtcccaa gcaataagag
     7981 gtcccaagga tatacatacg ccacacatag cgcaaatagt acataccaaa ggctgcaagt
     8041 agcaagtaaa ataggctaag aaggaggtcc tgctgggtta attgccccga tgtgatggca
     8101 tcaatgaccc gccccataac cataggagga atgagattga ggacggaaac caagatcagg
     8161 gccacaattc cgactagata acggcgtttt tctaacttga aaaaccacca aagtttttga
     8221 ataatggaca taaaatccct ttctgattgc aaatggaaac ctgaggccaa tactcaatga
     8281 aaatcaaaga gcaaactagg aaactagccg caggctgctc aaagcactgc tttgaggttg
     8341 tagatagaac tgacgaagtc agtaacctac atacggcaag gcgacgttga cgcggtttga
     8401 agagattttc gaagagtaca agaccccagg tttttcttat tcataagtta cgactgtaac
     8461 agcacccttg tcatattcag caataaagat attggctaca ttgtcatgcc cttgtttact
     8521 gaggttatca agcaaccact cttcgctacg accaatcgat tccaagacat ctacttgaat
     8581 cacaccgtca gtcacaactg gatacttagg attttcatct cccatttgca caacgatgag
     8641 ttgcccattt tgctcttgca cagctcgttt gacttgcttc atctggaaaa tcccttggct
     8701 acgaagtttg agggatacat ccgatgcaga caaaccaact gaacgacagg cttctgggtc
     8761 aatattccca tttttgataa ggagagttgg cttaccatca atcaaacgtt tcacaaagtg
     8821 aacattgttg ttgagctatt tgagagtcaa gaccagtttg attaaaattt cgatataatt
     8881 aagtgtcatt tgttcacatc caccagttca attgcgtctg tcttatacaa ttcgactttc
     8941 tctaacaggt acttgtctgg ctcacttccg tttagggcac gataatagcg atctcccacc
     9001 ttgataagtg cgccatctgt gtttgtggaa gtattaacat agacttctga cttgtctact
     9061 cacaaatctt tggaaacaac ctcgatgaaa tgaagtgaag tttgaaattt attgttagaa
     9121 acttgattgt tttgataagt cgtgatgctg accaaaagca gagctaaata aggccagatc
     9181 tgaaatcatg accaactcac gaaattcgct tccattttta ttatggtatg ccttgaaagc
     9241 aaaaaaaact gtcacagcta atagaacaat cccaaagcca accatgatac cattttgctg
     9301 accgatttgg ctgagtacat agtcataaga gtagaatttc atgtattttc actccctttc
     9361 ataaaatcat tcttaattat aaacgaaaga gagtaatttt tcaaaccata cgttggggaa
     9421 atagatcttt ttttggtata ataaaatcta taatctgaat gaaaaaggta actttatgaa
     9481 acttatctca tggaatattg attccctaaa cgctgcccta actagtgact cagctcgtgc
     9541 caaattgtcc caagaagtcc tacaaacctt ggtcgctgaa aatgctgata tcattgctat
     9601 ccaagaaacc aagctttctg ccaaaggacc tacaaagaaa cacgtggaaa ttttagaaga
     9661 actcttccca ggctacgaaa acacgtggcg ctcttcccaa gagcctgccc gtaaaggcta
     9721 tgctggaacc atgttccttt ataagaaaga acttacacct actatcagct tcccagaaat
     9781 cggtgcccct tctaccatgg acttggaagg tcgtatcatc actctagaat ttgatgcatt
     9841 tttcgtaacc caagtttaca ctccaaacgc tggtgacggt ctcaaacgct tggaagaacg
     9901 ccaagtctgg gatgccaaat atgctgagta tttggctgaa ctagacaaag aaaaaccagt
     9961 ccttgcgacc ggtgactaca acgtagccca caatgaaatc gaccttgcaa atcctgctag
    10021 caaccgccgt tcacctggat ttactgacga ggaacgtgct ggatttacca accttttggc
    10081 aactggattt acagacacct tccgccatgt tcatggcgat gttcctgaac gctacacttg
    10141 gtgggcacaa cgcagcaaaa cttctaaaat caacaataca ggctggagaa tcgactactg
    10201 gctcacaagt aaccgtatcg ctgacaaggt cactaaatct gatatgattg actcaggtgc
    10261 tcgccaagac catacaccga ttgtcttgga aattgacttg taaggatttt ctatatggac
    10321 tacaatgcgg tcattcccga gttgcttgta tcgaatatcg aacagtcccg gtccttctac
    10381 tgtggtttac tgggttttag gattgaatac taacgtccag aagaaaactt tctctttctc
    10441 ttgaagagtg tcaactaatg ttagaggagg gaacaaagga tcaactagct gaactgacct
    10501 acccctttgg tcgtggagtc aatctctcct ttggcataaa ggatgttcca aaactctatc
    10561 aaaaagtgat ggaggcaaac tatcctattt atcgtctctt gaccaaaaga aagtttcgtg
    10621 tcagtgatcc ctatatctat cctcataaat ttgcagtttt ggatccagat ggctattttt
    10681 taagatttag cgagtagaat aataaaaatt gggaaaagtc tttaaagtta gaaaaaggca
    10741 tattgtcagg tgtctaaaaa cttgatagta tgccttttat gatggaaaaa tcttctaata
    10801 ctcttcgaaa atctcttcaa gccacgtcaa cgtcgcctta ccgtaggtat atgatactga
    10861 ctctgtcaat tctatccaca acctcaaaac agtgttttga ggttgtggat agcttcctag
    10921 tttgctcttt gattttcatt tagtataaat gttagcttac aatggagttt ttgttcaccc
    10981 tctcttctgt ttgttcgttt tttaaaccaa aacactaaca aaatagttga tttctaaagt
    11041 tatttagtgt aaaataaaaa cattggctca agcctattta aaattgaata tcgttttact
    11101 tgtttatgtc gtgaattggc acgacgtttc tacaaggtgc cggaacacct aacaataagt
    11161 aagtcagcag tgagatatgg ccgtttttgc catgtctatt taggggactt acttagagat
    11221 taagtaggtc ttttttgaat atttttaata tttcccttaa ttagtgcgga cgggaggtga
    11281 aattatcctg tgaataattt caggaatcca gggaatttca tccctaattt ttgagcccct
    11341 agctctttca tttctaggct taggctaaaa tagttccccg aactatttta ctctcaaaaa
    11401 tcccgtcata gacaataaca agattattgt ctatgatacc actacaacgg gaaatatttg
    11461 ataagactcg cttcgctcgt aatagtttta tcaaatatat agtattagca aaagggatca
    11521 ctatagaaac atttaaacaa caagtaaatt aacttttaga ctttaggagg tcttatgaaa
    11581 ttattagaag agcgcatcct caaggatggg catatcttgg gtgataacat cctcaaggta
    11641 gattcctttt taacccacca agttgacttt agcttgatgc aagagattgg taaggttttt
    11701 gcggaaaaat ttgctgctac tggcattacc aaggtcgtaa ccattgaagc gtcgggtatt
    11761 gccccagccg tttttacagc tgaagcctta aacgttccca tgattttcgc caaaaaagct
    11821 aagaacatca ccatgaacga aggcatctta actgctcaag tctactcctt taccaagcag
    11881 gtgaccagca ctgtttctat cgctggaaaa ttcctctcac cagaggacaa ggttttgatt
    11941 atcgacgatt tccttgctaa tggccaagct gctaaaggct tgattcaaat catcgaacag
    12001 gccggtgcca cagtccaagc tatcggtatc gtgattgaga aatccttcca agatggtcgt
    12061 gatttgcttg aaaaagcagg ctaccctgtc ctatcacttg ctcgtttgga tcgttttgaa
    12121 aatggtcaag tcgtatttaa ggaggcagat ctctaatgca aactcaagaa aaacactcgc
    12181 aagcagccgt tcttggcttg cagcacttac tagccatgta ctcaggatct atcctggttc
    12241 ccatcatgat tgcgacagcc cttggctatt cagctgagca gttgacctac ctgatttcta
    12301 cagatatctt catgtgtggg gtggcaacct tccttcaact ccaactcaac aaatactttg
    12361 ggattggact cccagtcgtt cttggagttg cattccagtc ggtcgctccc ttgattatga
    12421 ttgggcaaag ccatggtagt ggcgctatgt ttggtgccct tatcgcatct gggatttacg
    12481 tggttcttgt ttcaggcatc ttctcaaaag tagccaatct tttcccatct atcgtaacag
    12541 gatctgttat taccacgatt ggtttaacct tgatccctgt cgctattgga aatatgggaa
    12601 ataacgttcc agagccaact ggtcaaagtc tcttgctttc agctattact gttctgatta
    12661 tcctcttgat caacatcttt accaaaggat ttatcaagtc tatctctatt ttgattggtc
    12721 tggttgttgg aactgccatt gctgctacta tgggcttggt ggacttctct cctgttgcgg
    12781 tagctccact tgtccatgtc ccaactccac tctactttgg gatgccaacc tttgaaatct
    12841 catctattgt catgatgtgt atcatcgcaa cggtgtctat ggttgagtca actggtgttt
    12901 atctagcctt gtctgatatc acaaaagatc caatcgacag cacgcgcctt cgcaacggtt
    12961 accgcgcaga aggtttggcc gtacttctcg gaggaatctt taacaccttc ccttacaccg
    13021 gattttcaca aaacgttggt ttggttaaat tgtcaggcat caaaaaacgc ctgccaatct
    13081 actacgcagc tggtttcctg gttctccttg gactgcttcc taagtttggc gcccttgccc
    13141 aaatcattcc aagctccgtc ctcggtggtg ccatgctggt gatgtttggt tttgtatcta
    13201 ttcaagggat gcaaatccta gcccgagttg actttgctaa caatgaacac aacttcctta
    13261 tcgcagctgt ttcaatcgct gcaggtgtcg gtctcaacaa cagtaatctc tttgtcagca
    13321 tgccgacagc cttccaaatg ttcttctcaa acggaatcgt cgtagccagc ctattcgcta
    13381 ttgtcctcaa tgccgtatta aatcataaaa agaaataaga aaaagaggtg cgagcctctt
    13441 ttttgtttat tacaaattga ttataaatac aatcaatccc aattcccatc taattgaatt
    13501 ggacctattt caaattcttt aaaa
//
LOCUS       DAVKQL010000052        12921 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVKQL010000052 DAVKQL010000000
VERSION     DAVKQL010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12921
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.052"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(<1..141)
                     /locus_tag="VW561_001610"
     CDS             complement(<1..141)
                     /locus_tag="VW561_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943844.1"
                     /translation="MDLLEKECLKCDKNFQQGDIWNYYHLSDKMPAQGWKIHISFQIK
                     DAV"
     gene            691..1368
                     /locus_tag="VW561_001611"
     CDS             691..1368
                     /locus_tag="VW561_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEU9943845.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            1355..2152
                     /locus_tag="VW561_001612"
     CDS             1355..2152
                     /locus_tag="VW561_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEU9943846.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGAERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWKLEILPSQASTNPFRHI"
     gene            2169..3272
                     /locus_tag="VW561_001613"
     CDS             2169..3272
                     /locus_tag="VW561_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU9943847.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQKIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEASQVKLVKEKVTLTPLASGYQIGEEEFEQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLVQDMEAYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDETLLQQME
                     EATLTHYLILAEATSKSERVGIRAYTSDFSPFFGQVPDLTGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPENYPIENYVKRVKSE"
     gene            3342..3515
                     /locus_tag="VW561_001614"
     CDS             3342..3515
                     /locus_tag="VW561_001614"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943848.1"
                     /translation="MDRTDEVSSKHCFEVVDRTDEVSSKHCFEVVDRTDEVSNHTHGK
                     ATLTWFELDFRRV"
     gene            3618..4637
                     /gene="galE"
                     /locus_tag="VW561_001615"
     CDS             3618..4637
                     /gene="galE"
                     /locus_tag="VW561_001615"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9943849.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTATLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            4639..5604
                     /locus_tag="VW561_001616"
     CDS             4639..5604
                     /locus_tag="VW561_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9943850.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTHYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKNKVKRL"
     gene            complement(5655..5861)
                     /locus_tag="VW561_001617"
     CDS             complement(5655..5861)
                     /locus_tag="VW561_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEU9943851.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            5913..6389
                     /locus_tag="VW561_001618"
     CDS             5913..6389
                     /locus_tag="VW561_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEU9943852.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            6398..7069
                     /locus_tag="VW561_001619"
     CDS             6398..7069
                     /locus_tag="VW561_001619"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEU9943853.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            7200..7538
                     /locus_tag="VW561_001620"
     CDS             7200..7538
                     /locus_tag="VW561_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEU9943854.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            7541..8170
                     /locus_tag="VW561_001621"
     CDS             7541..8170
                     /locus_tag="VW561_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEU9943855.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITKHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            8410..9561
                     /locus_tag="VW561_001622"
     CDS             8410..9561
                     /locus_tag="VW561_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9943856.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            9874..10623
                     /gene="truA"
                     /locus_tag="VW561_001623"
     CDS             9874..10623
                     /gene="truA"
                     /locus_tag="VW561_001623"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEU9943857.1"
                     /translation="MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            10613..11386
                     /locus_tag="VW561_001624"
     CDS             10613..11386
                     /locus_tag="VW561_001624"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU9943858.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            11364..11825
                     /locus_tag="VW561_001625"
     CDS             11364..11825
                     /locus_tag="VW561_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9943859.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
     gene            12052..12921
                     /locus_tag="VW561_001626"
                     /pseudo
     CDS             12052..12921
                     /locus_tag="VW561_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         3785 a         2391 c         2913 g         3832 t
ORIGIN      
        1 tacagcatct ttgatttgaa aacttatgtg tattttccag ccttgtgcag gcatcttatc
       61 tgacaaatga tagtaattcc aaatatcgcc ctgttggaaa tttttatcac attttaaaca
      121 ttctttctct aataaatcca taacaaactc ctaattatta aaataggcta gagacacagc
      181 actagcctat tccgtaagta aagctactta ttttatataa aatataacta gcgacctgta
      241 attaaacaag taaaagaaca atacttaggg agacctgtga caatgacatc ttcctctgga
      301 agtagttcta attcaaacaa tgtttgttcc atgactgtat cttcctgtta tgataaattc
      361 cagaaagcat agagcaaatt cgtggttcgt ctgactaaat ttcttatctg aacaccacct
      421 tcaaatacaa attttagtca aacttttatt ttttctaact ttctaattat attctaccat
      481 gtgtttttta tatgtcaaga tttttctgaa taaactgttt attttttatt ttttgacatt
      541 tttgcaaatt gtttaaaatc tttcaattgg attcacttat tactttagga cattgaaact
      601 tctttcgcca gaggtgctta tattactggg aagttgaaga ttgaaatgtg gtataataag
      661 aggtaataga gttttggaaa gtgagagaag atgatttcaa agagattaga attggtagct
      721 tcctttgtgt cacagggggc tattttacta gatgtgggaa gtgaccatgc ttatctgcct
      781 atcgagttgg ttgagagagg ccaaatcaaa agcgctattg caggtgaggt ggtggaaggt
      841 ccctatcagt ctgcggttaa aaatgttgag gctcacggcc taaaggagaa aatccaagtc
      901 cgtttagcca atggcttggc agcttttgaa gagactgacc aagtgtctgt cattaccatt
      961 gctggcatgg gtggtcgttt gattgctagg attttagaag aaggtttggg gaagttagct
     1021 aatgtagagc gtttgatcct ccagcccaat aatcgtgaag acgacttgcg tatctggcta
     1081 caggatcatg gattccagat tgtagcagaa agcatcttag aagaagctgg aaagttttat
     1141 gagattttgg tggtggaagc aggacaaatg aagctatcag ccagtgatgt tcgctttggt
     1201 cccttcttgt ccaaagaagt cagtccagta tttgtccaaa aatggcaaaa agaagctgag
     1261 aagctagagt tcgccctcgg acaaatccca gaaaaaaatc tggaagaacg tcaagttcta
     1321 gtagataaga ttcaagctat caaggaggtg ctccatgtta gcaagtgaag tgatccaagc
     1381 ttatgaagcc ttttgccctc aggaattttc tatggaggga gacagccgtg gtctgcaaat
     1441 tggcacttta gacaagggta tccaaagggt catggttgcc ctcgatattc gtgaagagac
     1501 ggtggcagag gccattgaaa agggtgtgga cttgattatc gtcaagcacg cgcctatctt
     1561 tcgtcctatc aaggacttgc ttgccagccg tccgcaaaat cagatttaca tcgacctgat
     1621 taagcatgac atcgcagttt atgtcagcca taccaatatt gatatcgttg aaaatgggct
     1681 caatgactgg ttttgtcaga tgctaggaat cgaggagacg acttatctgc aggaaacagg
     1741 tgcagaacgt ggaattggac gtattgggaa tattcagcct cagacatttt gggaattggc
     1801 ccaacaggtc aagcaagtct ttgacctaga tagccttcga atggtgcatt atcaagagaa
     1861 tgatttgcag aagcctattt caagagtagc aatttgtggt ggaagcgggc agtctttcta
     1921 taaggatgct ttggcaaagg gggcagatgt ctatatcact ggtgatatct actaccatac
     1981 tgctcaggat atgctgtctg atggcttgtt agcattggat ccaggtcact atatcgaagt
     2041 gctttttgtg gaaaaaatcg cagcactcct tactcaatgg aaagagaaaa aaggctggaa
     2101 actagagatt ttacctagtc aagcatcgac caatcctttc cgtcatatct agttagaaag
     2161 tgaagacaat gaaaaaagtt gccattattg gagcaggaat tgtgggagca acagctgcct
     2221 actacctctc gagagaaagt gacctagagg tgaccgtttt tgaccatgag caaggtcaag
     2281 ccaccaaggc cgcagcagga attatcagtc cttggttttc caaacgccgt aataaagcct
     2341 ggtacaagat ggcgcgcttg ggggctgatt tttatgtgga tttattagct gatttagaga
     2401 aatcaggaca aaaaatcgac ttttaccagc gttcgggagt ctttctcttg aaaaaggatg
     2461 aatccaagtt ggaagaactt tatcaattgg ccctccagcg cagagaagaa tctcccttga
     2521 tagggcaatt agccattctg gaccaagcct cagctaatga attattccct ggtttgcagg
     2581 gatttgaccg cctgctctat gcttctggtg gagcgagagt agatggccaa ctcttggtaa
     2641 ctcgtctgct agaagccagt caggtcaagc tggtcaaaga aaaagtgact ctgacaccgt
     2701 tagcatcagg ctaccagatt ggtgaagagg agtttgagca ggttattttg gcgacgggag
     2761 cttggttggg ggacatgtta gaacccttgg gttatgaagt agatgttcgt cctcaaaaag
     2821 gacaactacg agattatcag ctggtccaag acatggaagc ttaccctgtt gtcatgccag
     2881 aaggggagtg ggatttgatt ccttttgcag gtgggaaatt gtccttaggc gctacccacg
     2941 aaaatgacat gggatttgat ttgacggtag atgaaacctt gctccaacaa atggaggagg
     3001 ccaccttgac tcactatctg attttggctg aagctacttc aaaatctgag cgtgttggaa
     3061 tccgtgccta caccagtgat ttctctcctt tctttgggca ggtgcctgac ttaactggtg
     3121 tctatgcagc cagtggacta ggttcatcag gcctcacaac tggtcctatt attggttacc
     3181 atttagctca actgatccaa gacaaggagt tgaccttgga ccctgaaaac tacccaattg
     3241 aaaactatgt caaacgagta aaaagcgaat aatactcaat gaaaatcaaa gaacaaacta
     3301 ggtaactagc cgcgggttac tcaaagcact gctttgaggt tgtggataga actgacgaag
     3361 tcagctcaaa acactgtttt gaggttgtgg atagaactga cgaagtcagc tcaaaacact
     3421 gttttgaggt tgtagataga actgacgaag tcagtaacca tacccatggc aaggcgacgt
     3481 tgacgtggtt tgaattggat tttcgaagag tataagcatt ttactgaaat tttatcaggt
     3541 agtttaggat ggcaaatgac attccctgtc aaaaatggta aaataagaaa aaataatctg
     3601 agaatcgagg aaaaaagatg caagaaaaga ttttggtaac tggtggtgcc ggttttattg
     3661 gaacccacac tgttattgaa ttgatccaag caggccatca agttgttgtg gtggataatc
     3721 ttgtcaatag caatcgtaag agtttagaag ttgttgaaag aatcacagga gttgagattc
     3781 ctttctatga ggcagatatt cgtgacacag ctaccctcag agatattttc aagcaggaag
     3841 aaccgactgg tgtcattcac tttgctggtt tgaaggctgt tggcgaatca acacgtatcc
     3901 ctcttgccta ctatgacaac aatatcgctg gaactgtcag ccttttgaaa gccatggaag
     3961 aaaacaactg taaaaacatc atcttcagtt cttctgcgac agtttacggg gatccgcaca
     4021 cagtgcccat cttggaagat ttcccacttt cagtgaccaa cccatacggt cgtactaagc
     4081 tcatgctaga ggaaattttg actgatattt acaaagcaga ctcagaatgg aatgttgtct
     4141 tgcttcgtta ctttaaccca atcggagccc atgagagtgg tgatttggga gaaaatccaa
     4201 acggtattcc aaacaatctc ttgccatatg tgactcaagt agccgttgga aaattagagc
     4261 aagtgcaagt gtttggagac gattacgata cggaagatgg aacaggtgtt cgtgactata
     4321 tccacgttgt cgatttggct aagggtcacg ttgcagcttt gaaaaaaatc caaaaaggtt
     4381 caggactaaa cgtttataac cttggaactg gtaaaggtta ctcagttctt gaaattatcc
     4441 aaaacatgga aaaagcggtg ggacgtccta ttccttaccg catcgtagaa cgtcgcccag
     4501 gtgatatcgc tgcctgctat tcagacccag caaaagctaa agcagaactc ggttgggaag
     4561 cagaactcga catcacccaa atgtgtgaag acgcatggcg ttggcaaagc aagcatccaa
     4621 atggatttga agactaagat gatgatttca atcatcgtcc cttgtttaaa cgaagaggaa
     4681 gtacttcctc ttttttatca ggctctggaa gctttacttc cagatttgga aacagaaatc
     4741 gagtatgtct ttgtcgatga tggatcaagt gatgggacct tggaactctt aaaggcctat
     4801 cgggagcaaa atccggcagt ccattatatt tctttctctc gaaattttgg caaagaagca
     4861 gccctttatg caggcttgca atatgcgaca ggagatttgg tggtggtgat ggatgcagac
     4921 ctccaagatc ctcctagtat gttgtttgag atgaaaaatg tactagacaa aaatgtagac
     4981 ttggactgcg ttgggacacg gagaactagt cgggagggag aacccttctt tcgcagtttc
     5041 tgtgctgttc tcttttatcg cctcatgcaa aaaatcagcc cagtagctct gccgtcgggt
     5101 gtccgtgatt ttcgtatgat gagaaggtct gtggtcgatg ccattttaag tttgactgag
     5161 tccaatcgtt tttctaaggg actctttgcc tgggtcggct ttaaaaccca ctatctggac
     5221 tatccaaatg tcgaaaggca ggctggcaag accagttgga gttttaggca actctttttc
     5281 tactccattg aagggattat taatttttca gattttccct tgattatagc ctttgtagct
     5341 ggtctcctat cttgttttct ttctctgctg atgacctttt ttgttgtggt tcggaccctc
     5401 attttgggca atccgacatc gggttggacc tctctgatgg ctgtcattct ctttcttgga
     5461 ggcattcaac tcttgaccat tgggattctc ggcaagtata tcagtaagat ttatttagag
     5521 actaaaaaaa gaccacttta tcttatcaaa gaaaaaagtg accttcctga ttttacagaa
     5581 aaaaataaag tgaaaagact ataattttac atggaaatgt gctaaactag agggagtgga
     5641 ttatcgccat tgatttaggc ttcctggttt ccaatcaggg cgcgagttgg gcaattttta
     5701 acagcctcta agacatcctg actaggagaa atttcttttt ccagttggtc agggtcatcg
     5761 taaaaacgca cgattccatt atcgtggtaa tcaaataaat cagaataagt ttggcaaagc
     5821 ccacaggcga tacatcgttc aggtataagt gtgattttca tgttcatatt tatattgtaa
     5881 taagaaaagt aaaaaaaaac aaggagtaag gtatggcaaa agaaccgtgg caagaagata
     5941 tctatgatca agaagaatca agagcagagc gtcggcatcg aaaccacgga ggggctgata
     6001 ggatggctaa tcgtattttg acgatcctag ctagtatttt ctttgtaatt gtggtggtga
     6061 tggtcatcgt tctcatctat ctatcatcgg ggggaagtaa tcgcacagca gccttaaaag
     6121 actttcatga ttctgatgca agtgtagtac aaatctcatc ttcaagcagt tctcagcctg
     6181 agcagagttc agagccagaa tctacttcta gtagttcaga agaagctgct aatcctgaag
     6241 gaacgattaa agttctcgca ggagaagggg aagcagctat tgccgctcgt gcaggaatct
     6301 ccattgctca gttagaggcc ttgaatcctg ggcacatggc tacaggatct tggtttgcta
     6361 atccaggtga tgttataaaa ataaaatagg agccgatatg aaaacaattc aaattgctat
     6421 tgatggtcct gcttccagcg gtaagagtac ggtcgcaaag attattgcta aggattttgg
     6481 attcacctac cttgatacag gagctatgta tcgtgcagcg acctatatgg ctcttaagaa
     6541 ccaattagga gttgaagaag tcgaagccct tctagccttg ttggaccagc atccaatcag
     6601 ctttggacgt tcagaaactg gagaccagct tgtttttgta ggagatgtgg atattaccca
     6661 tcctatccgt gaaaatgaag tgaccaatca tgtttctgct attgcagcaa ttcctgaagt
     6721 gcgtgagaaa ctggtttctc tccaacaaga aattgcccag caaggcggga ttgtcatgga
     6781 tggtcgcgat attggaactg ttgtattgcc acaagcagaa ttgaaaattt tcctagtagc
     6841 ttctgttgat gagagagcag agcgtcgtta caaggaaaat attgccaagg gaattgaaac
     6901 agaccttgaa accctaaaaa aggaaattgc tgcgcgtgac tacaaggata gtcatcgtga
     6961 gacttctcct ctcaaacaag cagaggatgc tgtctacctt gatacaactg gtttgaacat
     7021 tcaagaagta gttgaaaaaa tcaaagcaga agctgaaaaa agaatgtaaa tgtagaaaag
     7081 ccgatagatt gatatcggtt tttttctaaa tttgtcaaaa tgctgatttt agggtataat
     7141 agttgctgtt cgagaaattt tgattttcaa agaatagtga atgatgataa ggagaaacca
     7201 tgaacaattt accaaattgt ccaaaatgta actcagagta tgtctacgaa gacggtgccc
     7261 tactggtttg cccagagtgt gctcatgagt ggaatcctgc tgaagttgca gaagtagaag
     7321 aaggtcttgt cgctatcgat gccaacggaa ataaattgac tgatggtgat acagtaactc
     7381 tcatcaagga cttgaaagta aaaggtgcgc caaaagattt gaaacaaggg acgcgcgtga
     7441 aaaatatccg catcgtagaa ggcgaccaca atatcgactg taagattgat ggttttggtg
     7501 ccatgaaact taaatcagag tttgtgagga agatttaagc atgtcaaaac actataaact
     7561 tgtattttat agccgtatct tcttgtttct agcggctttt acgggagttt atcttgaaat
     7621 tactaagcat ggtggttttg ggatgcttct ctattacacg gttctgtcca acctcttggt
     7681 aactattttt accctttatc ttctaaaggt tatgagccgt gtaggtgaaa attggcaaag
     7741 accaagtctc ttgcgcttaa aaggtggggt caccatgagt atcatgatta cctgtgtgat
     7801 ttaccatttc ctcttggcgc ccattgcgac taatttctat accctagaaa atttcctttg
     7861 ccactatatc gttcccatct ggtttttagc ggataccctc ttttttgaca aacagggtca
     7921 atacaagatt tgggatccag cagtgtggac cattttaccc tttctgtata tgatgtttgc
     7981 tctttttaat ggcttggttc taaaactcaa tattccaaat gccaaggata atcctttccc
     8041 ttacttcttt ttgaatgtga acaagggttg gaatgttgtg tttaagtggt gtctgattat
     8101 ctttgttgcc tatatggtag caggatttat tttctacttt atcaagcaaa tcaagagaaa
     8161 gtcatcctaa tactcttcga aaatctcttc aaaccacgtc agtttccatc tgcaacctca
     8221 aaacactgtt ttgaacaacc tgcggctagc ttcctagttt gctctttgat tttcattgag
     8281 tataagatac aagggattca ttttcgaatt taagaaggag tcgacaaacc aatgaggctt
     8341 cttcttttct tgcttatttg ataactatca aaaaagtaga agattaagag aaatatagaa
     8401 agagatttca tgaaacaatt tttagaacgg gccagcattt tggctctctc cctcgttttg
     8461 attacctcct tttccatttc gagtgcccta ccagccatgt ttgactatta tcagggttat
     8521 tctaaggaac aaattgagct cttggtgagc ttgccttcct ttggaatcat gatgatgtta
     8581 ctgctaaatg gtttcttaga aaaaatattt cctgagcgct tacagattag tttgggcttg
     8641 ctgattttat cattgagcgg tacagctccc ttctggtacc aagcctatcc ctttgtcttt
     8701 ggaacacggc ttctctttgg tttgggtctt gggatgatca atgccaaggc catttctatt
     8761 atcagtgaac gctaccaagg aaaaaggcga attcagatgt tagggctacg cgcttctgca
     8821 gaggtcgttg gagcttctct cattaccttg gccgtcggtc agttgttggc ctttggttgg
     8881 acagctatct ttctagccta tagtgctgga tttttggtgc tgccccttta tctgctcttt
     8941 gtcccttatg gaaaatcaaa gaaagaagtc aagaaaagag cgaaggaagc aagtcgttta
     9001 actcgagaaa tgaaaggctt gatttttacc ttagctatcg aagcggcagt tgtagtttgt
     9061 accaatacag ctattaccat ccgtattcca agtttgatgg tggaaagagg attgggggat
     9121 gcccagttat ctagttttgt tcttagtatc atgcagttga tcgggattgt ggctggggtg
     9181 agtttttctt tcttgatttc tatctttaaa gagaaactgc tcctctggtc tggtattacc
     9241 tttggcttgg ggcaaatcgt gattgccttg tcttcatcct tgtgggtggt agtagcagga
     9301 agtgttctgg ctggatttgc ctatagtgta gtcttgacga cggtctttca acttgtctct
     9361 gaacgaattc cagctaaact cctcaatcaa gcaacttcat ttgctgtatt aggctgtagt
     9421 ttcggagcct ttacgacccc atttgttcta ggtgcaattg gcttactaac tcacaatggg
     9481 atgttggtct ttagtatctt aggaggttgg ttgattgtaa tctctatctt tgtcatgtac
     9541 ctacttcaga agagagctta ggattgattc ctaagttttt cttttgatac tcaatgaaaa
     9601 tcaaagagca tagttgattg agtttggaat agtacgctgt agctgctaaa atatttctag
     9661 aaattaattt gacttttcta atagagttat tcatatctta tttcaatcta ctatagaaag
     9721 ctagccgcag gttgctcaaa atagtgtttt gaggttgcag atggaaactg acgtggtttg
     9781 aagagatttt cgaagagtat gagattttta tacccataca atatttgttt taggtccttg
     9841 tttacagact aatttcttga taaaatagag gttatgacga gatataaagc aactatttcc
     9901 tatgatggtt atgcctttgc tggctttcag cgccagtctc atgcgcgtag cgttcaggaa
     9961 gaaattgaaa aaaccttgac cagattaaat aaagggcaaa ccattactgt tcacggtgct
    10021 ggtaggacag atagtggggt tcatgccctg ggacaggtca ttcattttga cctgccttat
    10081 cagatggatg aggagaaact ccgttttgcc ttggataccc agtctcctga agatattgat
    10141 gtgatttcga ttgagcttgt ggcagatgat tttcattgcc gttatgccaa acatagcaag
    10201 acctatgagt ttattgtgga tagaggacgt cccaaaaatc ctatgcgccg tcactatgcc
    10261 acccactttc cctacccact cgatgtggaa cgaatgcaga ttgcaatcaa aaagctagag
    10321 ggaacccatg attttaccgg ttttacagcc tctgggacta gtgtagagga taaggttcgc
    10381 accatcacag aagctagttt aatagtcgat gagacaggac aatttttgac ctttaccttt
    10441 tcaggaaatg gtttcttgta taaacagatt cgcaatatgg tggggactct tctcaaaatc
    10501 ggtaacaacc gcatgccagt agagcagatt gatctcatct tggagaagaa ggacaggcaa
    10561 cttgcaggtc ccactgcagc accaaatggt ttgtatttaa aggagattcg ttatgaagaa
    10621 taatcgtatt ttagcacttt ctggaaatga tatttttagt ggtggtggac tgtcagctga
    10681 tttggctacc tataccttga acggcttgca tgggtttgta gcagtgactt gtttgacagc
    10741 cttgacagaa aaaggatttg aagtctttcc aactgatgat accatttttc aacatgaatt
    10801 agatagctta cgtgatgtgg aatttggggg aattaagatt ggtcttctcc ctactgtcag
    10861 tgtggctgag aaggccttgg actttatcaa acaacgccca ggagtacctg tggtgttgga
    10921 tcctgtcttg gtctgcaagg aaacgcatga tgtagctgtc agtgagctct gccaagagtt
    10981 gattcgcttt ttcccttatg tcagtgtgat tacgcctaat ctcccagaag cagaattatt
    11041 atccggtcag gaaattaaaa ccttggaaga catgaaaact gcagcgcaga aattgcatga
    11101 tttaggagcg ccagcagtca ttatcaaggg aggcaatcgt cttagtcagg acaaggctgt
    11161 ggatgtcttt tatgatggac agacctttac tatcctagaa aatccagtta tccaaggcca
    11221 aaatgctggt gcaggttgta cctttgcctc tagcattgcc agtcacttga ttaaaggtga
    11281 taaacttttg ccagcagtag aaagctctaa ggctttcgtt tatcgtgcta ttgcacaagc
    11341 agatcagtat ggagtaagac aatatgaagc aaacaaaaac aactaaaatc gcccttgtat
    11401 ccctattaac cgccctttct gtggttctag gttatttctt aaaaatccca acacctacag
    11461 gaattctaac tcttttagat gctggtgtct tctttgcggc cttttacttt ggtagtcgtg
    11521 aaggagcggt agtcggagga ctagcaagtt tcttgattga cctcttatca ggctaccctc
    11581 agtggatgtt ctttagcttg gtcaaccatg gcttgcaggg atttttcgca ggatttaaag
    11641 gaaaaagtca gtggttaggc cttattttag caactattgc catggtagga ggctacgcct
    11701 tgggttctac tttgatgaat ggctgggcag cagccctccc agaaattcta ccaaatttca
    11761 tgcaaaatat ggtagggatg attgtaggat ttattcttag tcaaagtatc aagaagatta
    11821 agtaatactc ttcgaaaatc tcttcaaacc acgtcagctt ccatctgcaa cctcaaaaca
    11881 ctgttttgag cagcctgagg ctagctttct agtttgcact ttgattttca ttgagtataa
    11941 agaggttgag taaaaagtat ttcaaataag aaaaacgcat agtatcaggt gttgaatgaa
    12001 ctgcccccca aaagttagac agaaaaaatc taacttttgg ggtgttttat tatgaaacta
    12061 acttatgatg ataaagttca gatctatgaa cttagaaaac aaggatatag cttagagaag
    12121 ctttcaaata aatttgggat aaacaattct aatcttaggt acatgattaa attgattgat
    12181 cgttacggaa tagagttcgt caaaaaaggg aaaaatcgtt actattctcc tgatttaaaa
    12241 caagaaatga ttcataaagt ctgacatgaa ggctagacta aagatagagt ttctcttgaa
    12301 tacggtctcc caagtcgtac gatacttctt aactggctag cacaatacag gaaaaacggg
    12361 tatactattg ttgagaaacc aagagggaga gtacctaaaa tgggacgtaa gccaagaacg
    12421 agacctgaag agaggacaga attagaacgt cttcaagcag aaaatgagta cctgagagcg
    12481 gagaatgcca tcctaaaaaa gttaagagaa ctccgattga aggaggaaaa agagaaagaa
    12541 gaaagacaga aattgttcaa gaattaatga ctgagttttc gttagctctt cttctaaaag
    12601 ccattaaact agctcgttgg acctactact atcacttgaa acagctagat aaaccagata
    12661 aggaccaaga gcttaaagct gaaattcaat ccatttttat cgaacacaag ggaaattatg
    12721 cttatcgctg tattcattta gaactaagaa atcgtgctta tctggtaaat cataaaagag
    12781 ttcaaggctt gataaaagta ctcaatttac aagctaaaat gcgacagaaa cgaaaataaa
    12841 ggagacgttg gtaagaaggc agagaatctc attcaaggcc aatttgaagg ctctaaagca
    12901 atggaaaagt gctacacaga t
//
LOCUS       DAVKQL010000053        12701 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVKQL010000053 DAVKQL010000000
VERSION     DAVKQL010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12701
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.053"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(112..549)
                     /locus_tag="VW561_001627"
     CDS             complement(112..549)
                     /locus_tag="VW561_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU9943860.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKSYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(634..3267)
                     /gene="polA"
                     /locus_tag="VW561_001628"
     CDS             complement(634..3267)
                     /gene="polA"
                     /locus_tag="VW561_001628"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEU9943861.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAEGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIMVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            3523..3873
                     /locus_tag="VW561_001629"
     CDS             3523..3873
                     /locus_tag="VW561_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEU9943862.1"
                     /translation="MLRHPGISPTNDLIAKKIFSNPEITCQFIRDMLDLPAKNVTILE
                     GSDIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQVN
                     QNLEKIRQREGDTH"
     gene            4088..4204
                     /locus_tag="VW561_001630"
     CDS             4088..4204
                     /locus_tag="VW561_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU9943863.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            4197..4430
                     /locus_tag="VW561_001631"
     CDS             4197..4430
                     /locus_tag="VW561_001631"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943864.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
     gene            4557..4838
                     /locus_tag="VW561_001632"
                     /pseudo
     CDS             4557..4838
                     /locus_tag="VW561_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4972..5940)
                     /locus_tag="VW561_001633"
     CDS             complement(4972..5940)
                     /locus_tag="VW561_001633"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU9943865.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(6085..6708)
                     /locus_tag="VW561_001634"
     CDS             complement(6085..6708)
                     /locus_tag="VW561_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEU9943866.1"
                     /translation="MPKKVLILSLLGGMFLSGWLSSFANTYIHDLLGVLFPDSTFLNA
                     FESAIVAPLVEEPLKLLPLVFVLALIPVRKLKSLFLLGIASGLGFQMIKDIGYIRTDL
                     PEGFDFTISRILERIISGIASHWTFSGLAVVGVYLLYRAYKGQKVGKKQGLIFLGLAL
                     GTHFLFNSPFVELETELPLAIPVVTAIALYGFYHAYCFVEKHNELMT"
     gene            complement(6690..6899)
                     /locus_tag="VW561_001635"
     CDS             complement(6690..6899)
                     /locus_tag="VW561_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943867.1"
                     /translation="MKKSSILFIFILLLCIGLQYETIYYTDGSRSGAEYGLMGVSIFL
                     ALFYMIPALYFLFRIGKNGNCQRRF"
     gene            complement(6924..7421)
                     /locus_tag="VW561_001636"
     CDS             complement(6924..7421)
                     /locus_tag="VW561_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEU9943868.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(7494..8855)
                     /gene="radA"
                     /locus_tag="VW561_001637"
     CDS             complement(7494..8855)
                     /gene="radA"
                     /locus_tag="VW561_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEU9943869.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(8869..9384)
                     /locus_tag="VW561_001638"
     CDS             complement(8869..9384)
                     /locus_tag="VW561_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9943870.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(9386..9829)
                     /locus_tag="VW561_001639"
     CDS             complement(9386..9829)
                     /locus_tag="VW561_001639"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEU9943871.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            complement(9886..9983)
                     /gene="ffs"
                     /locus_tag="VW561_001640"
     ncRNA           complement(9886..9983)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VW561_001640"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(10016..10483)
                     /locus_tag="VW561_001641"
     CDS             complement(10016..10483)
                     /locus_tag="VW561_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEU9943872.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYNILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(10684..11970)
                     /locus_tag="VW561_001642"
     CDS             complement(10684..11970)
                     /locus_tag="VW561_001642"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEU9943873.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(12201..12437)
                     /locus_tag="VW561_001643"
     CDS             complement(12201..12437)
                     /locus_tag="VW561_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HEU9943874.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCRKDFIVYHYRVAYRLYLEKLVMNRGFISC"
BASE COUNT         4002 a         3061 c         2136 g         3502 t
ORIGIN      
        1 gtatctacaa cctcaaaacg gtgttttgag cagcccgcgg ctagtttcct agtttgctct
       61 ttgattttca ttgagtataa cacaaaaggt agcccatcag ctaccttttt cttatgcttc
      121 ctcaatcaag cgagtatgtt ctctcttgat acagcgattc atcacgatat catcacatcc
      181 accatcacgc aaaatctctt ttgcttctaa actttcaagt cctagctgtg cccaaaaaat
      241 cttggcatca gctttgagaa aatcacgcgc cacatcgggc agaaattcac tgcgacgata
      301 aacattgaca atatctacag gaaaaggaat ttcagcgagg ctagcataag acttttcacc
      361 caagatttcg ccacctgccg ccttgggatt gactgggatg attttatagc cccgagcctg
      421 catttccttt gtcactcgat tgctggttgt ttcttcacgg tcagacaaac ccaccacagc
      481 aagggtttta ctcgttgcga gatactgacg aatcacgcca tcacttggat tgataaattc
      541 ttgactcata gaaatcctcc tttttcatca gtatagcaca ttttgaaaag gtttgcagaa
      601 ttctactaca aaaaaggagg actagccccc tttttattta gcctcgtacc aggttgcccc
      661 ttcattctca tctgcgataa gaggaacact gagttgaatg gcttcttcca tggtttgttt
      721 caccaatttt ttcatctcta ccaattcaga tttaggcact tcaaggacga tttcatcgtg
      781 cacttgtaac agcatcttag tctgataacc acctgcaacc aaggctttat ccagctgaat
      841 catggcaatc ttgagaatat ctgctgccga accctggata ggtgagttga tagcagttcg
      901 ctccgcaaaa ccacgaatat tgaagttgcg cgaattgata tctggcaact cacggcgacg
      961 cttaaagagg gtctctacat agcccttatc acgcgcctcc cgcaccactt catccatgta
     1021 gtttttaata cctggaaaac gttcaaagta ggtatcaatg taggctttgg cttccttacg
     1081 actaattccc aaattattag acaagccaaa gtctgaaatc ccataaacca ctccaaagtt
     1141 aactgccttg gcattgcgac ggtcatttgc agtcacatta tcaggacgct caatgccaaa
     1201 gacccgcatg gctgtcgaag tatggatatc tgccccctct tggaaggcct taatcaagtg
     1261 ctcatcctta gaaatatgcg ccaaaacgcg caattcaatc tgtgaatagt cagagctgag
     1321 tagcacacta tcctcccact ctggcacaaa agccttccga atcaagcgcc cttgttccaa
     1381 tcgggcagga atattttgca agtttggatc cacactagac aaacgcccgg tctgggtcaa
     1441 atcctgcata tagcgagtat gaatctttcc atcagccaaa atccagtcct gcaagccaat
     1501 tacataagta gattgaatct tagcaatttg acggtaatcc aggattttct taacaatcgg
     1561 agcaatagga gcgagacgct ccaacacatc cactgctgtc gaataacctg tcttggtttt
     1621 cttagtgtat tctagaggaa gtcccaattt ctcaaagaga agcacgccca actgcttagg
     1681 cgagttgaca ttaaactcct caccagccag ctcgtaaatc tcttgagtca gtttttcaat
     1741 gacaagctca ttttcagcct gcatctcaag caaggtctct ttcttgacca taatcccagc
     1801 aatttccatc ttggcaagga caaaagccag aggttgctcc atatcataaa gaagctctaa
     1861 ttgcccattt tcgctgagtt tttcaagtaa aataggctct gtttctacca aaacagcaag
     1921 tttacaagct aagtgttcca agaatttctc acgttcagga atggcctttt taacaccctt
     1981 accgtagaaa gtttcatcat caaccaagta agtctgacca taaagactag cgatggtcgc
     2041 aatttcattg tcctccacag tcgaaaggag gtatttagcc aaacggatgt caaaagcagg
     2101 cgcctgcaaa tccacaccaa aacgttgcaa aagaacttta accttcttaa agtcataaac
     2161 tctcagagat gttttttcta agaaatcctt gaaaatcggg tcttgcaaca gctcaagctt
     2221 gtctgtggca tagagcttat ccccacaaga ccaggcaaat ccaaccaaat tatccgtatg
     2281 gtaattctca ccaaaaagct caaagtggaa gatagactct tcactcagca tatcttgact
     2341 gatttggtca acaatagtaa aatccaaacc ctcagccaca tcagctgacg acacatttaa
     2401 agcctgcttg agctgtttga atcccatctc atcgtaaaat ttcccaagat tttcaacatc
     2461 tggaccacta tagaccaagt cctctaaacc aatcgcaatc ggtgccttgg tatcaatggt
     2521 cgctagtgtt ttagacaaaa aggcctgttc cttgtcatta atgagatttt ccttcatctt
     2581 agaagtcttc attccatcaa tattttcata aatcccctca agcgaaccat gctccagcaa
     2641 gagcttaata cccgtctttt caccgacttt ggtcacccca gggatattat ccgacttatc
     2701 acccatgagc gccttgagat cgataaactg agctggtgtg aggcccattt cttccatgag
     2761 gtaatctggc gtaaaggcct caaactcagc cacacctttc ttggaaattt caaccaccgt
     2821 atgctcatcc gtcagctgaa tcaaatcctt gtccccactg acaatggtaa tatcaaaacc
     2881 atcctgctct gctagcttat ccagcgtccc aatgatgtca tccgcctcat actgagccag
     2941 ctcatagtga cgaatcccca tatgatccag caactcacga atgaaaggaa attgctcacg
     3001 aaactcatca ggagtcttgg cccgaccacc cttatagtcc gcatacatct ctgtccggaa
     3061 ggtcgtcttt cccgcatcaa aagccaccaa aatatgactc ggctcaaccc gctccaacaa
     3121 atgactcaac atcaactgaa aaccataaat cgcattggta tgcaaaccag ccgcattctt
     3181 aaaacggtcc aactgctgat acagcgcaaa aaacgcccga aaagctacag aagacccatc
     3241 aatcaataat aattttttct tatccataca cccattataa aggaaagaat caaaaaatac
     3301 cattgggaag agctagagct agtatttttc aaactttttc cgaataaata gatagagcca
     3361 gagaatttag taaacctaga tttaaaaatg tgctataata tagtatattg aatctataat
     3421 agtacacctt gactactaaa atatttctat aaattaattt gactttcctg atagagttat
     3481 tcacatctta tttcaactca ctatagaagg aggaatagga ggattctcag acatccgggc
     3541 atcagcccaa ctaatgattt gattgctaag aaaatattca gcaatccaga aatcacttgt
     3601 caatttattc gcgatatgct ggacttgcca gcaaaaaatg tgaccatttt ggagggaagc
     3661 gatattcacg tattactctc catgccttac tcggtgcagg atttttatac cagtatagac
     3721 gtcttggcgg agttggataa cggtactcaa gtaattattg agattcaagt ccatcatcag
     3781 aattttttca tcaatcactt gtgggcttac ctgtgcagtc aggttaatca aaatcttgaa
     3841 aaaattcgtc agcgagaagg tgatactcac tagatctaca aacacatcgc tcctgtttac
     3901 gtcattgcta tcgtggatag taattatttc tcagatgacc tggcttttca tagctttagt
     3961 atgcgcgaag acacaacagg tgaggtattg gcgattacca acaatggaca ggaaaaccat
     4021 ctggttaaga tggcattctt ggaattaaaa aatacagaga aaccagcaaa gacaaggttc
     4081 gcaagccatg gttggagttt ttcggcaaca agccctttac ccagcaaccg caacgagcca
     4141 ttacccaagc aaatcaactg ctggactaca agagctggtc cgaggaggac aggaaaatgt
     4201 ttagtcaact acatatgcga gaagaacaag tcttgttagc acaggactat gccttggaaa
     4261 ctgctagggc tgaaggcctt gaacaaggac tagagcgtgg gaaagttgaa ggaagggcag
     4321 aaaggaaact ttttgccttc ctagacatag tacgccaagg tcttctgact tctgaggttg
     4381 ccagccagca attaggtatg tcagtatctg aatttgaggc actgttgtaa aatggctcca
     4441 taatatccat aggggattta cccactatgg atattatgga gcctattttg tgtaaaaaaa
     4501 aggtcccata tgacctataa tgaaaagcga caaaacaact cattagaaag aatcatatgg
     4561 aacaattaca ttttatcaca aaattactag acattaaaga ccctaatgtc cagattttaa
     4621 acatcatcaa taaggataca cacaaggaaa tcatcgccaa actggactac gacgccccat
     4681 cttgccctga gtgcggaaac caattgaaga aatatgactt tcaaaaacct tctaaaattc
     4741 cttatcttga aacgactggt atacctacta gaattctcct tagaaagcgt cgattcaagt
     4801 gctatcactg ttcaaaaatg atggtcgctg aaacttctga tgacgtacag tcatatttct
     4861 tctcttttta ttatatcaca gttttaaatc tagctttact agattcaccg ctactatcta
     4921 tttattcgga aaaaagacga aaaaacctga gaatcatctc aggcttggtc attaaatttt
     4981 tttctcaata tcgaaaagtg gagaaagtgg tcgtttttca tgaatacgta cgatagcatc
     5041 ccctaggaga tgagcgattg aaatctgctc aatcttatca atcaaacgct cttctggcag
     5101 atagatggta tccaaaacaa ccaatttctt aatagctgat ttttggatat tgtccgtagc
     5161 aggaccagaa agaactgggt gcgtacagct tgcatagact tcaacagcac cagcttccgc
     5221 aagagcatct gccgcatgac aaatcgttcc agcggtatca atcatatcat caatcaagat
     5281 acaagtcttg ccttcaacct taccgatgat gttcataact tcactagtat tcatcttatc
     5341 aacgctacga cgtttatcaa taatagcgat agatgttttc aaaaattctg ccaacttacg
     5401 agcacgagtc acccctccat ggtctgggct gacaaccaca tagtcagaac caaccatacc
     5461 acgacgctca aaataatctg caattagagg agcacccatc aaatgatcca caggaatatc
     5521 gaagaatcct tgaatttgcg cagcatgcaa gtcgatggtc aataaacgat ccactccagc
     5581 tacttcaagc atatttgcga caagttttga agtgattggc tcacgcgctc tcgcctttct
     5641 atcctgacgt gcatacccat agtaaggcat gacaacattg acagattctg cactcgcacg
     5701 cttcaaagca tctaccataa tcaaaatttc aagcagattg tcatttacag gcgaactagt
     5761 tgattgtagg ataaagacgt gtttcccacg gattgattct tcaatgttga cctgaatctc
     5821 tccatctgaa aattggcgaa cacttgattt ccccaactct atcccaatct cctgcgccac
     5881 acgttctgcc aattctttat tagaagaaag ggcaaacagc tttaaatcag aaaaagacat
     5941 gatttcctcc ggtatatatg tataacttgt gcttttcaca agattttcca tctaccattg
     6001 tagcgctttt tgcactattt ttcaatcaaa aataaaagaa gagcaccata tttgtaccct
     6061 tgcatcattc ttttgaaaaa tattctaggt catcaactca ttgtgtttct caacaaagca
     6121 ataagcatga taaaaaccat agagagcaat agccgtaacc actggaatcg ctaaaggcaa
     6181 ctctgtttcc aactccacaa aaggagagtt aaacaagaag tgagttccca aggctaaacc
     6241 tagaaaaata aggccctgtt tcttgccaac cttctgtcct ttataggctc tgtaaagcaa
     6301 gtaaacacct actacagcta gacctgaaaa agtccagtga gaggcaattc ctgagatgat
     6361 acgctctaaa attcgcgaaa tagtaaagtc aaagccctct ggcaaatccg tacgaatata
     6421 accaatatcc ttaatcattt ggaatcccaa accggaagca attccaagta aaaacaaaga
     6481 ttttaatttt cgcacaggaa tcaaagccaa aacaaaaaca agtggcaata atttcaaggg
     6541 ttcttctacc aaaggagcca caatagcact ttcaaaggca tttaaaaatg tactatctgg
     6601 gaaaagaacc cccagtaaat catggatata agtattagca aaactagaca accagcctga
     6661 aaggaacatc cctcccaata aagacagaat caaaaccttc tttggcaatt cccatttttc
     6721 ccaatacgga agagaaaata aagagccgga atcatgtaaa agagagctag aaagatagaa
     6781 actcccatta gtccatattc cgcacctgac ctcgaaccgt ccgtatagta gatggtttca
     6841 tactgtaaac caatacatag caataaaata aaaataaata aaatactgct tttcttcata
     6901 cactttcttt ctaaatgaag tatttataat tctacgactg tcatacttcc tgtatcaaca
     6961 ttgtaaatgg caccagagat aatgacatcg tctggtatta ggggagactc gataagcagt
     7021 tgcatatcct cgcgtacact ctcttctata tcttggaagg gcaagaagtc ctggtctgac
     7081 acatctacac ctaattcctc ttttaaatac tcctgaaaag gttcattttc aaaggtctga
     7141 gcaccacagt ctgtatggtg caataccaca atctctcttg tccccatttg ttgctgggaa
     7201 ataactagcg aacgaatcat gtcttcagtc actcgaccac ctgcattccg caagatatga
     7261 gcatccccaa gtgccaaacc tagagcttgc gcaacgtgca aacgtgagtc catacaggtc
     7321 acaatggcta ctctggtttt aggtttaagt ggcagattta actgcccatg tagggcaaca
     7381 taagcctgat tggcttgcat aaactgttca aaatacgaca cgattccctc cttgaaaatt
     7441 tgatagtcaa atatttctcc tatcttatca tttttaagag aatttgtcac ggattatgca
     7501 aagacctttt tcaaaacttc ctgaatcgtt gtcacgccaa tgacctgaat ttccttaggc
     7561 agagtgattc ctgtcaagga attcttaggt acataaatct tagtaaagcc cagtttagca
     7621 gcttcgttga tgcgttgctc aatacgattc acgcgccgaa tctctcctgt caagcccagt
     7681 tctccgacaa aacattcctg aggattagtt ggcttgtctt tgtagctcga agcaatagca
     7741 actgcaacag ccaagtcaat cgcaggttca tccaatttaa caccaccagc agatttgaga
     7801 taggcatcct gattttgcaa gagaagccct gcccgttttt ccaaaacagc cataatcaag
     7861 ctagcacggt taaaatcaag tcctgtcgtc gtacgcttgg catttccaaa catggtcggt
     7921 gttaccaaag cctgaacctc cgccaaaatc ggacgcgtcc cttccatggt tacaacgatg
     7981 gaggaaccag tcgccccatc caaacgctct tctaggaaaa cttgactcgg attgagtacc
     8041 tcaaccaagc cgcccgactg catctcaaaa atcccaatct cattagtgga accaaaacga
     8101 tttttgaccg ctctcaaaat acgaaaggtg tggtgacgct ccccttcaaa gtaaagcacc
     8161 gtatccacca tatgctccaa catacgaggc ccagccaagg ttccttcttt ggtcacatga
     8221 cctacgataa agatggcaat gttattggtc ttggccaact gcatgagttc agcggtcact
     8281 tcacgtacct gagaaacaga cccctgcacc cctgaaatct caggagacat gatggtctgg
     8341 atggaatcaa taatgagaaa gtctggctgg atacgctcca cttctgcacg aacactctgc
     8401 atattggtct ctgcatagag ataaaactca ctatcaatat cacctaagcg ctctgcacgt
     8461 agtttaatct gctgggcaga ctcctcccca ctgacataga gaactgtccc cacttgggac
     8521 aactgggttg agacttgtag gagaagagtt gatttcccaa tcccaggatc cccaccgatg
     8581 aggacgagac ttcctggtac cactccgcct ccaagcacac ggttgaattc ctccatctcc
     8641 gtcttggttc gattgacatt gatggaagtc acctcagcta gtttcatggg cttggttttc
     8701 tcacctgtca aggacacacg cgcattcttg acctcggcaa cctcaacctc ttccacaaaa
     8761 gaagaccaag acccacagtt ggggcaacgt cccagatatt taggggaatt atacccacaa
     8821 ttttgacata caaatgtcgc ttttttcttt gcgatgacaa acctctttct atatctctaa
     8881 ctcacactca atcacttggc aaaaatcaat cttctcattt ggcacaaact ggcgcatgag
     8941 cattcgatga gcaacaacta ccacagtctg atgttctcga tacttagaca tacattctag
     9001 aaaccgagac ttcatttccg tagctgtctc atattgaata ggactattag gaagcaactc
     9061 ccccttgttt tctaaaaaca gtcttctagc tgtttcaaag ttttctattc ctgttttata
     9121 gacctgccat tcatgtaata aaggctctac tcttaaagga agacccgtag cacagaccac
     9181 atacgaagcc gtttctaaag ctcttgtgac tgcagaagat acgattattt cagctgacga
     9241 gagtaaagga tttttgctca atttctggac ttgctgccgt cccatctcag acaagggtgc
     9301 caaatctatc ccaaatccta tataagaacg ctcctctaac tcacggtaat ctggctcccc
     9361 atgacgtaca aagataatct tcattctagt gccctgtcga tccaaatcca ccagttcgaa
     9421 cgccatcagc tgcatctcca tctgcaatta agaaagtagc aaaaacagcc tggacaatac
     9481 gttccccaac ttcaagaaca acctcttgat ctgtgatatt cttcatctgc gcaaaaatat
     9541 gcccttcatt tccaggattt ccataataat ccccatcaat gaccccaact gagttaatta
     9601 aaaccaagcc cttcttacga ggatttgaag aacgatcata gaggtagaga acctcagtcg
     9661 gctgcatata agccttaacc cctgtcggaa ccaagacaat ctctcctggc gcaacaactg
     9721 tacgcacagc aacctttaag tcgtaaccag ccgcatgcgc tgtctcacgc ttgggcaata
     9781 aattttcatc tgtaaaactc gaaaccaatt caaaaccacg aattttcata attttctctt
     9841 ttctattatc atttattcta gattattcta tcttatttat tcggaaaaag cacgaaaaaa
     9901 agagcacaca attcaaatcg cttagggctg ctggattcct cccctgaccc gcttcacgca
     9961 gaactgttgc tccactattt attatatcac attcctattc attttaaaag caaaattatt
    10021 ttttccgtct atttctaaaa aaatcctgca tgatagctgc gcattcatct tccaaaattc
    10081 ctatttcaac ctccacacga tgattgagac gctcatctgt caagatattg tacaaactcc
    10141 cagcagcgcc aaatttctgg tttttagccc catagaccac atttggaatg cgggcaagcc
    10201 caatcgctcc actacacatg acacaaggtt caatggtcac aaaaagtgtg caatccagca
    10261 agcgccagct ctcctcactc aagttcgcat cctctatagc cataatttcc gcatgcataa
    10321 ccgctcgctg taattcctca cgcgcattat gcccacgacc aatgatttcc ccatctttga
    10381 caatcacaca accaattgga atttcatcgt gttcaagagc aatctcagcc tctctcaaag
    10441 cctccctcat aaagacttct ttttcttcaa gtgtataata catctctttt ctcttttcct
    10501 acttatcgat tttattatta tatcatgaat cccaacacaa aaaaagccac cgaatgcggt
    10561 gactctatag ggagattatt atgaaaaaga aaagtttagg atatttgtta caacaagtta
    10621 ggaggtcttc ttgtaaccgt ttatagtata cccgacctat cttaaacaaa tcttaaaaat
    10681 ctcttaggac caaacacttt ctaaaatatt tgtttgttca cgaccaggac ctactgagaa
    10741 agtagaaata cgaacgccaa ccaattcact cacacgacga acatagttac gcgcattctc
    10801 aggaagatct tccaaattgc gaactccggt aatatcttct gaccaacctg gcaactcttc
    10861 atagataggc ttgcaacgtt tcaattgctc aagactagct ggatagtagt caatacgttg
    10921 accgtcaaga tcataggcca cacagatttt cacagtatcc aaaccgctca aaacatcaat
    10981 agagttcaaa gaaaggttag taataccaga aacacgacgg ctatgacgca tcacaactga
    11041 gtcaaaccaa cctacacgac gtggacgacc agttgttgta ccatactcat gacccacttc
    11101 acggatacgt tctcccactt catcaaacaa ctcagttggg aaaggaccat ctcctacacg
    11161 actcgtataa gctttacata cacctacaac cttgtcaatc ttgcttggac cgacaccaga
    11221 accaattgtc acaccaccag ctacagggtt tgatgacgta acaaatggat aagtaccttg
    11281 gtcgatatct agcataacac cttgtgcacc ttcaaaaagc acacgtttgc cattatcaag
    11341 cgcatcattc aagataacag atgtatctat cacgtatttc ttgatttgtt gaccatattc
    11401 gtaatattct tcaaaaatat catcgaaaac aatcgcttta ctgtcataca atttttcaaa
    11461 aagacgattc ttttcagcaa ggttacgttc taaacgctca cggaaaatat ctttatctaa
    11521 aagatctgca atacgaattc caacacgagc agccttgtcc atataagctg gaccaattcc
    11581 cttaattgtc gtaccaatct tattgtcgcc cttagcttct tcttgcaagc gatccaactc
    11641 gatatgataa ggcaaaataa catgcgcacg atcagaaata cgcaagttat cagttgttac
    11701 accttcctca tgaagatagc tcaactcttt tacaagagat ttaggattta caaccatacc
    11761 attcccaatg acagatattt tttcagggaa gaaaatccca gatgggatca agtgcaactt
    11821 aaatttctta ccgtcaatca caatcgtgtg accagcatta tcaccacctt ggtaacgtgc
    11881 aatcacttct gcattcgctg aaaggaagtc tgtaatcttc cctttacctt catcacccca
    11941 ttgggtacct acaacaacaa ctgaagtcat aatcttgtct gagccctcag gctcttcctt
    12001 tctcacatac atggcaggac tctcacctgc aattatatct tacaatttat tataataaaa
    12061 aatcgccttt ttatcaagaa gaaacaatag aaagatttgc tatttccaac tattaaaaaa
    12121 tgatttagaa aaattactag ctatttacta tcatctttcc ataaaagagt aaattagttc
    12181 ggaaatttac taaaattacc tcaacaagaa ataaaacccc gattcattac caatttttca
    12241 agatacaaac gataagcaac acgataatgg taaacaataa aatccttacg acaaccaatg
    12301 ccatatctta ctaaataata aattaaaaat ttaaaatgaa catgttccca gtcaaaatta
    12361 tcaccaaatg taggaccata ctcttcttca atactatcat agaaattagc catctgctca
    12421 taaatttttt gtaacataat caaatactcc ttttcttttt tataaactta ttctaacaaa
    12481 aaattttaca cattcactat caattcctga attgttaaaa taccagcctc aacaagataa
    12541 aaataggaaa agttgacaaa atagaaacaa atttgctacc taaatttcaa acaattaaag
    12601 ttctaaaaag accgccttaa aacttttggg taaatcctat taaaactata atatttttat
    12661 ataggtatta tggttctgat tttctagtat aaaaaaattc a
//
LOCUS       DAVKQL010000054        12605 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVKQL010000054 DAVKQL010000000
VERSION     DAVKQL010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12605
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.054"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            <1..1135
                     /gene="dnaN"
                     /locus_tag="VW561_001644"
     CDS             <1..1135
                     /gene="dnaN"
                     /locus_tag="VW561_001644"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU9943875.1"
                     /translation="IHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGITL
                     IGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQNQ
                     IVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRPI
                     LTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDDI
                     EAVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSMER
                     ARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYLI
                     DSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            1200..1394
                     /locus_tag="VW561_001645"
     CDS             1200..1394
                     /locus_tag="VW561_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEU9943876.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            1478..2593
                     /gene="ychF"
                     /locus_tag="VW561_001646"
     CDS             1478..2593
                     /gene="ychF"
                     /locus_tag="VW561_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEU9943877.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARIIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            2664..3233
                     /locus_tag="VW561_001647"
     CDS             2664..3233
                     /locus_tag="VW561_001647"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU9943878.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            3234..6743
                     /gene="mfd"
                     /locus_tag="VW561_001648"
     CDS             3234..6743
                     /gene="mfd"
                     /locus_tag="VW561_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEU9943879.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASG
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTSICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKVPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNVDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LESLLIFGESLLEIKESKEENSI"
     gene            6801..7067
                     /locus_tag="VW561_001649"
     CDS             6801..7067
                     /locus_tag="VW561_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEU9943880.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            7060..7428
                     /locus_tag="VW561_001650"
     CDS             7060..7428
                     /locus_tag="VW561_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEU9943881.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATRLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            7548..8816
                     /locus_tag="VW561_001651"
     CDS             7548..8816
                     /locus_tag="VW561_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEU9943882.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKAWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            8813..10090
                     /gene="tilS"
                     /locus_tag="VW561_001652"
     CDS             8813..10090
                     /gene="tilS"
                     /locus_tag="VW561_001652"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEU9943883.1"
                     /translation="MRESDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ESIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            10094..10636
                     /gene="hpt"
                     /locus_tag="VW561_001653"
     CDS             10094..10636
                     /gene="hpt"
                     /locus_tag="VW561_001653"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEU9943884.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKAREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            10652..>12605
                     /gene="ftsH"
                     /locus_tag="VW561_001654"
     CDS             10652..>12605
                     /gene="ftsH"
                     /locus_tag="VW561_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU9943885.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEETGIQFFTPSVTKVEKFTSTI
                     LSADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDE"
BASE COUNT         4336 a         1895 c         2565 g         3809 t
ORIGIN      
        1 gattcatttt tcaattaata aaaatttatt tctacaagca ttaaatacta ctaagagagc
       61 tattagttct aaaaatgcca ttcctatttt atcaacagta aaaattgacg tgaccaatga
      121 aggtattact ttaattggtt caaatggtca aatttcaatt gaaaatttta tttctcaaaa
      181 aaatgaagat gctggtttgt taattacttc tttaggttcg atccttcttg aagcttcttt
      241 ctttatcaat gtagtatcta gtttacctga tgtaactctt gattttaaag aaattgaaca
      301 aaatcaaatt gttttaacca gtggcaaatc agaaattacc ctaaaaggaa aagatagcga
      361 acaatatcca cgaatccaag aaatttcagc aagcactcct ttaatacttg aaacaaaatt
      421 actcaagaaa attattaatg aaacagcctt tgctgcaagt acacaagaga gtcgtccgat
      481 tttaacaggt gtccacttcg tattgagtca acacaaagag ttaaaaacag ttgcaacaga
      541 ctctcatcgc ctaagccaga aaaaattgac tcttgaaaaa aatagtgatg attttgatgt
      601 cgtaattcct agccgttctc tacgcgaatt ttcagcggta tttacagatg atatcgaagc
      661 tgtagagatt ttctttgcca ataaccaaat cctctttaga agcgaaaata ttagcttcta
      721 tactcgtctc ctagaaggaa actatcctga tacagatcgc ttgattccaa cagactttaa
      781 cactactatt acttttaatg tggtaaactt acgccagtca atggagcgtg cccgtctttt
      841 atcaagtgcg actcaaaatg gtactgtgaa acttgaaatt aaggatgggg ttgttagcgc
      901 ccatgttcac tctccagaag ttggtaaagt aaacgaagaa atcgatactg atcaggttac
      961 tggtgaagat ttgaccatta gtttcaaccc aacttacttg attgattctc ttaaagcttt
     1021 aaatagcgaa aaggtgacca ttagctttat ctcagctgtt cgtccattta ctcttgtgcc
     1081 agcagatact gacgaagact tcatgcagct cattacacca gttcgtacaa attaagtgaa
     1141 agaggttgag cctggctcgc ctcttttatg atataatcga aaaagaaaag gagagtagta
     1201 tgtatcaagt tggaaatttt gttgagatga aaaaatcaca cgcttgtaca atcaagtcga
     1261 ctggtaaaaa ggctaatcgt tgggaaatta cacgtgtagg agcagatatc aaaataaaat
     1321 gcagtaattg tgagcatgtt gtcatgatgg ggcgatatga ttttgagcga aaaatgaata
     1381 aaattattga ctgagaaccc ttagttagag ggttagcact ttatcccttt ttgtgttata
     1441 atattaggga ttgaaatgaa aacggagaat gagaaatatg gctttgacag caggtatcgt
     1501 tggtttgcca aacgttggta aatcaacact atttaatgca attacaaaag caggagcaga
     1561 ggcagcaaac tacccatttg cgactattga tccaaatgtt ggaatggtgg aagttccaga
     1621 tgaacgccta caaaaactaa ctgaaatgat aactcctaaa aagacagttc ccacaacatt
     1681 tgaatttaca gatattgcag ggattgtaaa aggagcttca aaaggagaag ggctagggaa
     1741 taaattcttg gccaatattc gtgaagtaga tgcgattgtt cacgtagttc gtgcttttga
     1801 tgatgaaaat gtaatgcgcg agcaaggacg tgaagacgcc tttgtagatc cacttgcaga
     1861 tattgataca attaatctgg aattaattct tgctgactta gaatcagtga acaaacgata
     1921 tgcgcgtgta gaaaagatgg cacgtacgca aaaagataaa gaatcagtag cagaattcaa
     1981 tgttcttcaa aagattaaac cagtcctaga agacgggaaa tcagctcgta tcattgaatt
     2041 tacagatgag gaacaaaagg ttgtcaaagg tcttttcctt ttgacgacta aaccagttct
     2101 ttatgtagct aatgtggacg aggatgtggt ttcagaacct gactctatcg actatgtcaa
     2161 acaaattcgt gaatttgcag cgacagaaaa tgctgaagta gtcgttattt ctgcgcgtgc
     2221 tgaggaagaa atttctgaat tggatgatga agataaaaaa gagtttcttg aagccattgg
     2281 tttgacagaa tcaggtgtag ataagttgac gcgtgcagct taccacttgc ttggattggg
     2341 aacttacttc acagctggtg aaaaagaagt tcgcgcttgg actttcaaac gtggtatgaa
     2401 ggctcctcaa gcagctggta ttatccactc agactttgaa aaaggcttta ttcgtgcagt
     2461 aaccatgtca tatgaagatc tagtgaaata cggatctgaa aaggccgtaa aagaagctgg
     2521 acgcttgcgt gaagaaggaa aagaatatat cgttcaagat ggcgatatca tggaattccg
     2581 ctttaatgtc taaaaattaa taaatggtgt caattaggtt ggaaaaaaat tccaaccctt
     2641 ttggcttttg aaaggaaaaa taaatgacca aattacttgt aggcttggga aatccagggg
     2701 ataaatattt tgaaacaaaa cacaatgttg gttttatgtt gattgatcaa ctagcgaaga
     2761 aacagaatgt cacttttaca cacgataaga tatttcaagc tgacctagca tcctttttcc
     2821 taaatggaga aaaaatttat ctggttaaac caacgacctt tatgaatgaa agtggaaaag
     2881 cagttcatgc tttattaact tactatggtt tggatattga cgatttactt atcatttacg
     2941 atgatcttga catggaagtt gggaaaattc gtttaagagc aaaaggctca gcaggtggtc
     3001 ataatggtat caagtctatt attcaacata taggaactca ggtctttaac cgtgttaaga
     3061 ttggaattgg aagacctaaa aatggtatgt cagttgttca tcatgttttg agtaagtttg
     3121 acagggatga ttatatcggt attttacagt ctgttgacaa agttgacgat tctgtaaact
     3181 actatttaca agagaaaaat tttgagaaaa caatgcagag gtataacgga taaatggtga
     3241 ccttattaga tttattctca gaaaatgatc agattaaaaa atggcatcaa aatttaacag
     3301 ataagaaaag acaactaata cttggtttat caacatctac taaggcgctt gcaattgcaa
     3361 gcggtttaga aaaagaagat aggattgtgt tattgacgtc aacttatgga gaagcagaag
     3421 gacttgttag tgatcttata tctatcttgg gtgaggaact cgtctatcca tttttggtag
     3481 atgatgctcc tatggtggag tttttgatgt cttcacagga aaaaattatt tcacgggttg
     3541 aagccttgcg ttttttgact gattcatcta agaaagggat tttagtttgt aatatcgcag
     3601 caagtcgatt gattttaccg tctcccaatg cattcaaaga tagtattgta aaaatctcag
     3661 ttggtgaaga atatgatcaa cacgcgttta tccatcagtt aaaggaaaat ggctatcgaa
     3721 aagttactca agtacaaact cagggcgaat ttagtcttcg aggagatatt ttagatattt
     3781 ttgaaatatc ccagttagaa ccttgtcgaa ttgagttttt tggtgatgaa attgatggta
     3841 tcaggtcatt tgaagtagaa acacaattat cgaaagaaaa taagacagaa ctcactatct
     3901 ttccagctag tgatatgctt ttgagagaaa aggattatca acgaggacag tcagctttag
     3961 aaaaacaaat ttcaaaaact ttatcaccta ttttgaaatc atacctagaa gaaattcttt
     4021 caagttttca tcaaaagcaa agtcatgcag attctcggaa gtttttatct ttgtgctatg
     4081 ataagacatg gactgtcttt gattatattg aaaaagatac tccaatattc tttgatgatt
     4141 atcaaaaatt gatgaatcag tatgaagtat ttgaaagaga cttagcgcag tactttacag
     4201 aagaattaca gaatagtaaa gcattttctg atatgcagta tttttctgat attgaacaaa
     4261 tctataaaaa acaaagtcca gtgacctttt tctctaatct tcaaaagggt ttaggaaatc
     4321 tcaaatttga caaaatttat caattcaatc aatatcctat gcaggaattt ttcaatcagt
     4381 tttcttttct aaaagaagaa attgaacgat ataaaaaaat ggattacacc attattctgc
     4441 agtctagcaa ttcaatggga agtaaaacat tggaggatat gttagaggaa tatcagatta
     4501 aattggattc tagagataag acaagtatct gtaaagaatc tgtaaactta atagagggta
     4561 atctcagaca tggttttcat tttgtagatg aaaagatttt attgataact gaacatgaga
     4621 tttttcaaaa gaaattaaag cgtcgttttc gaagacaaca tgtttcaaat gcagagagat
     4681 taaaagatta caatgaactt gaaaaagggg actatgttgt ccatcatatc catgggattg
     4741 gtcaatatct aggaattgaa accattgaaa tcaagggaat tcatcgcgat tatgtcagtg
     4801 tccaatacca aaatggtgat caaatttcta ttcccgtgga acagattcat ctactgtcca
     4861 aatatatttc aagtgatggt aaagttccaa aactcaataa attaaatgat ggtcatttta
     4921 aaaaggccaa gcaaaaggtt aagaaccagg tagaggatat agctgatgat ttaatcaaac
     4981 tctactctga acgtagtcag ttgaagggtt ttgctttctc agctgatgat gatgatcaag
     5041 atgcctttga tgatgctttc ccttatgttg aaacggatga tcaacttcgt agtattgagg
     5101 aaatcaagag ggatatgcag gcttctcagc caatggatcg acttttagtt ggggatgttg
     5161 gttttggaaa gactgaagtt gctatgcgtg cagcctttaa agcagtcaat gatcacaaac
     5221 aggttgtcat tctagttccg acgacggttt tagcgcaaca gcactatacg aattttaagg
     5281 aacgattcca aaattttgca gttaatgttg atgtgttgag tcgctttaga agtaaaaaag
     5341 agcagactgc aacacttgaa aaattgaaaa acggtcaagt cgatattttg attggaacac
     5401 atcgtgtttt gtcaaaagat gttgtgtttg ctgatttggg cttgatgatt attgatgagg
     5461 aacagcgatt tggtgtcaag cataaggaaa ctttgaaaga actgaagaaa caagtggatg
     5521 tcctaacctt gaccgctacg ccaatccctc gtaccctcca tatgtctatg ctgggaatca
     5581 gagatttgtc tgttattgaa actccgccga ctaatcgcta tcctgttcag acctatgttt
     5641 tggaaaagaa tgatagtgtc attcgtgatg ctgtcttgcg tgaaatggag cgtggaggtc
     5701 aagtttacta tctttacaac aaagttgaca caattgttca gaaggtttca gaattacagg
     5761 agttgattcc ggaggcttcg attggatatg ttcatggtcg aatgagtgaa gtccagttgg
     5821 aaaatactct attagacttt attgagggac aatacgatat cttggtgacg actactatta
     5881 ttgagacagg ggtggacatt ccaaatgcta atactttatt tattgaaaat gcggaccata
     5941 tgggcttgtc aaccttatat cagttaagag gaagagtcgg tcgtagtaat cgtattgctt
     6001 atgcttatct catgtatcgt ccagaaaaat caatcagtga agtctctgaa aagagattag
     6061 aagcgattaa aggatttaca gaattgggct ctggctttaa gattgcaatg cgagatcttt
     6121 cgattcgtgg agcaggaaat cttttaggaa aatcccagtc tggtttcatt gattctgttg
     6181 gttttgaatt gtattcgcag ttattagagg aagctattgc taaacgaaac ggtaatgcta
     6241 acgctaacac aagaaccaaa gggaatgctg agttgatttt gcaaattgat gcctatcttc
     6301 ctgatactta tatttctgat caacgacata agattgaaat ttacaagaaa attcgtcaaa
     6361 ttgacaaccg tgtcaattat gaagagttac aagaggagtt gatagaccgt tttggagaat
     6421 acccagatgt agtagcctat cttttagaga ttggtttggt taaatcatac ttggacaagg
     6481 tctttgttca acgtgtggaa agaaaagata ataaaattac aattcaattt gaaaaagtca
     6541 ctcaacgact gtttttagct caagattatt ttaaagcttt atccgtaacg aacttaaaag
     6601 caggcatcgc tgagaataag ggattaatgg agcttgtatt tgatgtccaa aataagaaag
     6661 attatgaaat tttagaaagt ttgctgattt ttggagaaag tttattagaa ataaaagagt
     6721 ctaaggaaga aaattccatt tgatattttt cttctataaa atagataaaa atggtacaat
     6781 aataaattga ggtaataagg atgagattag ataaatattt aaaagtatcg cgaattatca
     6841 agcgtcgtac agtcgcaaag gaagtagcag ataaaggtag aatcaaggtt aatggaatct
     6901 tggccaaaag ttcaacggac ttgaaagtta atgaccaagt tgaaattcgc tttggcaata
     6961 agttgctact tgtaaaagta ctagagatga aagatagtac aaaaaaagaa gatgcagcag
     7021 gcatgtatga aattatcagt gaaacacggg tagaagaaaa tgtctaaaaa tattgtacaa
     7081 ttgaataatt cttttattca aaatgaatac caacgtcgtc gctacctgat gaaagaacga
     7141 caaaaacgga atcgttttat ggggggggta ttgattttga ttatgctatt atttatcttg
     7201 ccaactttta atttagcgca gagttatcag caattactcc aaagacgtca gcaattagca
     7261 gacttgcaaa ctcagtatca aactttgagt gatgaaaagg ataaggagac agcatttgct
     7321 accaggttga aagatgaaga ttatgctgct aaatatacac gagcgaagta ctattattct
     7381 aagtcgaggg aaaaagttta tacgattcct gacttgcttc aaaggtgata aaatggaaaa
     7441 tttattagac gtaatagagc aatttttgag tttgtcagat gaaaagctgg aagaattggc
     7501 tgataaaaat caattattgc gtttacaaga agaaaaggaa aggaagaatg cgtaaattct
     7561 taattatttt gttgctacca agttttttga ccatttcaaa agtcgttagc acagaaaaag
     7621 aagtcgtcta tacttcgaaa gaaatttatt acctttcaca atctgacttt ggtatttatt
     7681 ttagagaaaa attaagttct cccatggttt atggagaggt tcctgtttat gcgaatgaag
     7741 atttagtagt ggaatctggg aaattgactc ccaaaacaag ttttcaaata accgagtggc
     7801 gcttaaataa acaaggaatt ccagtattta agctatcaaa tcatcaattt atagctgcgg
     7861 acaaacgatt tttatatgat caatcagagg taactccaac aataaaaaaa gcatggttag
     7921 aatctgactt taaactgtac aatagtcctt atgatttaaa agaagtgaaa tcatccttat
     7981 cagcttattc gcaagtatca atcgacaaga ccatgtttgt agaaggaaga gaatttctac
     8041 atattgatca ggctggatgg gtagctaaag aatcaacttc tgaagaagat aatcggatga
     8101 gtaaagttca agaaatgtta tctgaaaaat atcagaaaga ttctttctct atttatgtta
     8161 agcaactgac tactggaaaa gaagctggta tcaatcaaga tgaaaagatg tatgcagcca
     8221 gcgttttgaa actctcctat ctctattata cgcaagaaaa aataaatgag ggtctttatc
     8281 agttagatac gactgtaaaa tacgtatctg cagtcaatga ttttccaggt tcttataaac
     8341 cagagggaag tggtagtctt cctaaaaaag aagataataa agaatattct ttaaaggatt
     8401 taattacgaa agtatcaaaa gaatctgata atgtagctca taatctattg ggatattaca
     8461 tttcaaacca atctgatgcc acattcaaat ccaaaatgtc tgccattatg ggagatgatt
     8521 gggatccaaa agaaaaattg atttcttcta agatggccgg gaagtttatg gaagctattt
     8581 ataatcaaaa tggatttgtg ctagagtctt tgactaaaac agattttgat agtcagcgaa
     8641 ttgccaaagg tgtttctgtt aaagtagctc ataaaattgg agatgcggat gaatttaagc
     8701 atgatacggg tgttgtctat gcagattctc catttattct ttctattttc actaagaatt
     8761 ctgattatga tacgatttct aagatagcca aggatgttta tgaggttcta aaatgaggga
     8821 atcagatttt ttaaatcatt ttctcaagaa gggatatttc aaaaagcatg ctaaggcggt
     8881 tctagctctt tctggtggat tagattctat gtttctattt aaggtattgt ctacttatca
     8941 aaaagagtta gagattgaat tgattctagc tcatgtgaat cataagcaga gaattgaatc
     9001 agattgggaa gaaaaggaat taaggaagtt ggctgctgaa gcagagcttc ctatttatat
     9061 cagcaatttt tcaggagaat tttcagaagc gcgtgcacga aattttcgtt atgatttttt
     9121 tcaagaggtc atgaaaaaga caggtgcgac agctttagtc actgcccacc atgctgatga
     9181 tcaggtggaa acgattttga tgcgcttgat tcgaggaact cgcttgcgct atctatcagg
     9241 aattaaggag aagcaagtag tcggagagat agaaatcatt cgtcccttct tgcattttca
     9301 gaaaaaagac tttccatcaa tttttcactt tgaagataca tcaaatcagg agaatcatta
     9361 ttttcgaaat cgtattcgaa actcttactt accagaattg gaaaaagaaa atcctcgatt
     9421 tagggatgca atcttaggca ttggcaatga aattttagat tatgatttgg caatagctga
     9481 attatctaac aatattaatg tggaagattt acagcagtta ttttcttact ctgagtctac
     9541 acaaagagtt ttacttcaaa cttatctgaa tcgttttcca gatttgaatc ttacaaaagc
     9601 tcagtttgct gaagttcagc agattttaaa atctaaaagc cagtatcgtc atccgattaa
     9661 aaatggctat gaattgataa aagagtacca acagtttcag atttgtaaaa tcagtccgca
     9721 ggctgatgaa aaggaagatg aacttgtgtt acactatcaa aatcaggtag cttatcaagg
     9781 atatttattt tcttttggac ttccattaga aggtgaatca attcaacaaa tacctgtttc
     9841 acgtgaaaca tccatacaca ttcgtcatcg aaaaacagga gatgttttga ttcaaaatgg
     9901 gcatagaaaa aaactcagac gtttatttat tgatttgaaa atccctatgg aaaagagaaa
     9961 ctctgctctt attattgagc aatttggtga aattgtctca attttgggaa ttgcgaccaa
    10021 taatttgagt aaaaaaacga aaaatgatat aatgaacact gtactttata tagaaaaaat
    10081 agataggtaa aaaatgttag aaaacgatat taaaaaagtc ctcgtttcac acgatgaaat
    10141 tacagaagca gctaaaaaac taggtgctca attaactaaa gattatgcag gaaaaaatcc
    10201 aatcttagtt gggattttaa aaggatctat tccttttatg gctgaattgg tcaaacatat
    10261 tgatacacat attgaaatgg acttcatgat ggtttctagc taccatggtg gaacagcaag
    10321 tagtggtgtt atcaatatta aacaagatgt gactcaagat atcaaaggaa gacatgttct
    10381 atttgtagaa gatatcattg atacaggtca aactttgaag aatttgcgag atatgtttaa
    10441 agcaagagaa gcagcttctg ttaaaattgc aaccttgttg gataaaccag aaggacgtgt
    10501 tgtagaaatt gaggcagact atacctgctt tactatccca aatgagtttg tagtaggtta
    10561 tggtttagac tacaaagaaa attatcgtaa tcttccttat attggagtat tgaaagagga
    10621 agtgtattca aattagaaag aataatcttt aatgaaaaaa caaaataatg gtttaattaa
    10681 aaatcctttt ctatggttat tatttatctt tttccttgtg acaggattcc agtatttcta
    10741 ttctgggaat aactcaggag gaagtcagca aatcaactat actgagttgg tacaagaaat
    10801 taccgatggt aatgtaaaag aattaactta ccaaccaaat ggtagtgtta tcgaagtttc
    10861 tggtgtctat aaaaatccta aaacaagtaa agaagaaaca ggtattcagt ttttcacgcc
    10921 atctgttact aaggtagaga aatttaccag cactattctt tctgcagata ctaccgtatc
    10981 agaattgcaa aaacttgcta ctgaccataa agcagaagta actgttaagc atgaaagttc
    11041 aagtggtata tggattaatc tactcgtatc cattgtgcca tttggaattc tattcttctt
    11101 cctattctct atgatgggaa atatgggagg aggcaatggc cgtaatccaa tgagttttgg
    11161 acgtagtaag gctaaagcag caaataaaga agatattaaa gtaagatttt cagatgttgc
    11221 tggagctgag gaagaaaaac aagaactagt tgaagttgtt gagttcttaa aagatccaaa
    11281 acgattcaca aaacttggag cccgtattcc agcaggtgtt cttttggagg gacctccggg
    11341 gacaggtaaa actttgcttg ctaaggcagt cgctggagaa gcaggtgttc cattctttag
    11401 tatctcaggt tctgactttg tagaaatgtt tgtcggagtt ggagctagtc gtgttcgctc
    11461 tctttttgag gatgccaaaa aagcagcacc agctatcatc tttatcgatg aaattgatgc
    11521 tgttggacgt caacgtggag tcggtctcgg cggaggtaat gacgaacgtg aacaaacctt
    11581 gaaccaactt ttgattgaga tggatggttt tgagggaaat gaagggatta tcgtcatcgc
    11641 tgcgacaaac cgttcagatg tacttgaccc tgcccttttg cgtccaggac gttttgatag
    11701 aaaagtattg gttggtcgtc ctgatgttaa aggtcgtgaa gcaatcttga aagttcacgc
    11761 taagaataag cctttagcag aagatgttga tttgaaatta gtggctcaac aaactccagg
    11821 ctttgttggt gctgatttag agaatgtctt gaatgaagca gctttagttg ctgctcgtcg
    11881 caataaatcg ataattgatg cttcagatat tgatgaagca gaagatagag ttattgctgg
    11941 accttctaag aaagataaga cagtttcaca aaaagaacga gaattggttg cttaccatga
    12001 ggcaggacat accattgttg gtctagtctt gtcgaatgct cgcgttgtcc ataaggttac
    12061 aattgtacca cgcggccgtg caggcggata catgattgca cttcctaaag aggatcaaat
    12121 gcttctatct aaagaagata tgaaagagca attggctggc ttaatgggtg gacgtgtagc
    12181 tgaagaaatt atctttaatg tccaaaccac aggagcttca aacgactttg aacaagcgac
    12241 acaaatggca cgtgcaatgg ttacagagta cggtatgagt gaaaaacttg gcccagtaca
    12301 atatgaagga aaccatgcta tgcttggtgc acagagtcct caaaaatcaa tttcagaaca
    12361 aacagcttat gaaattgatg aagaggttcg ttcattatta aatgaggcac gaaataaagc
    12421 tgctgaaatt attcagtcaa atcgtgaaac tcacaagtta attgcagaag cattattgaa
    12481 atacgaaaca ttggatagta cacaaattaa agctctttac gaaacaggaa agatgcctga
    12541 agcagtagaa gaggaatctc atgcactatc ctatgatgaa gtaaagtcaa aaatgaatga
    12601 cgaaa
//
LOCUS       DAVKQL010000055        12604 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVKQL010000055 DAVKQL010000000
VERSION     DAVKQL010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12604
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.055"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            204..473
                     /gene="blpJ"
                     /locus_tag="VW561_001655"
     CDS             204..473
                     /gene="blpJ"
                     /locus_tag="VW561_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HEU9943886.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
     gene            542..790
                     /gene="blpK"
                     /locus_tag="VW561_001656"
     CDS             542..790
                     /gene="blpK"
                     /locus_tag="VW561_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpK"
                     /protein_id="HEU9943887.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGAAVVAAL
                     GCAAGGVKYGKILGPWGAAIGGIGGAVVCGYLAYTATS"
     gene            1034..1183
                     /gene="blpO"
                     /locus_tag="VW561_001657"
     CDS             1034..1183
                     /gene="blpO"
                     /locus_tag="VW561_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HEU9943888.1"
                     /translation="MNTKMMSQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            1287..1406
                     /locus_tag="VW561_001658"
     CDS             1287..1406
                     /locus_tag="VW561_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEU9943889.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            1874..2245
                     /locus_tag="VW561_001659"
     CDS             1874..2245
                     /locus_tag="VW561_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943890.1"
                     /translation="MIKKNHILCEIVPGSVVFACFYLSISISSVFQFLLVVINLTSIL
                     VTVYLTREMRLREFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITAGESYQQLIAYI
                     GAILCLIIMLLLIWGLKYYKK"
     gene            2874..3257
                     /locus_tag="VW561_001660"
     CDS             2874..3257
                     /locus_tag="VW561_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEU9943891.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            3309..3998
                     /locus_tag="VW561_001661"
     CDS             3309..3998
                     /locus_tag="VW561_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9943892.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQMTLIIQNYWQFSSQIGNFV
                     WIQNFLSLLFSGVMIWILVKTGHGYLFHIPRKKWLWYSILTVLVVVLQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYITLFFLTPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFIFATSGIIFASLTRYTKSIYPSYAVHVINNIFATLP
                     FLLTFLHRVLG"
     gene            4040..4273
                     /locus_tag="VW561_001662"
     CDS             4040..4273
                     /locus_tag="VW561_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943893.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            4424..5035
                     /locus_tag="VW561_001663"
     CDS             4424..5035
                     /locus_tag="VW561_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9943894.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            5196..5990
                     /locus_tag="VW561_001664"
     CDS             5196..5990
                     /locus_tag="VW561_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEU9943895.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETASDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            5987..6622
                     /gene="trmB"
                     /locus_tag="VW561_001665"
     CDS             5987..6622
                     /gene="trmB"
                     /locus_tag="VW561_001665"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEU9943896.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDDEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            6748..7227
                     /gene="rimP"
                     /locus_tag="VW561_001666"
     CDS             6748..7227
                     /gene="rimP"
                     /locus_tag="VW561_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEU9943897.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVRIPYSLVSKARLAVKL"
     gene            7271..8407
                     /gene="nusA"
                     /locus_tag="VW561_001667"
     CDS             7271..8407
                     /gene="nusA"
                     /locus_tag="VW561_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEU9943898.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            8429..8722
                     /locus_tag="VW561_001668"
     CDS             8429..8722
                     /locus_tag="VW561_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEU9943899.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            8715..9014
                     /locus_tag="VW561_001669"
     CDS             8715..9014
                     /locus_tag="VW561_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEU9943900.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLAKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            9031..11907
                     /gene="infB"
                     /locus_tag="VW561_001670"
     CDS             9031..11907
                     /gene="infB"
                     /locus_tag="VW561_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU9943901.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAAPKASAEKKAE
                     KSEPVKPAVAKEEAKPAEPVTPKTEKVAAKPQSRNFKAEREARAKEQAERRKQNKGNN
                     RDQQQNGNRQKNDGRNGGKQGQSNRDNRRFNDQAKKQQGQQKRRNERRQQEDKRSNQA
                     APRIDFKARAAALKAEQNAEYARSSEERFKQYQAAKEALAQANKRKEPEEIFEEAAKL
                     AEQAQQVQAVVEVVPEKKEPAVDTRRKKQARPDKNRDDYDHEEDGPRKQQKNRSSQNQ
                     VRNQKNSNWNNNKKNKKGNNKNNRNQTPKPVTERKFHELPTEFEYTDGMTVAEIAKRI
                     KREPAEIVKKLFMMGVMATQNQSLDGETIELLMVDYGIEAKQKVEVDNADIERFFVED
                     GYLNEDELVERPPVVTIMGHVDHGKTTLLDTLRNSRVATGEAGGITQHIGAYQIVENG
                     KKITFLDTPGHAAFTSMRARGASVTDITILVVAADDGVMPQTIEAINHSKAANVPIIV
                     AINKIDKPGANPERVIGELAEHGVMSTAWGGDSEFVEISAKFNQNIEELLETVLLVAE
                     IQELKADPTVRAIGTVIEARLDKGKGAVATLLVQQGTLNVQDPIVVGNTFGRVRAMTN
                     DLGRRVKVAGPSTPVSITGLNEAPMAGDHFAVYEDEKSARAAGEERAKRALMKQRQAT
                     QRVSLENLFDTLKAGELKSVNVIIKADVQGSVEALSASLQKIDVEGVKVTIVHSAVGA
                     INESDVTLAEASNAFIVGFNVRPTPQARQQAEADDVEIRLHSIIYKVIEEMEEAMKGM
                     LDPEFEEKVIGEAVIRETFKVSKVGTIGGFMVINGKVARDSKVRVIRDGVVIYDGELA
                     SLKHYKDDVKEVTNGREGGLMIDGYNDIKMDDVIEAYVMEEIKR"
     gene            12158..12508
                     /gene="rbfA"
                     /locus_tag="VW561_001671"
     CDS             12158..12508
                     /gene="rbfA"
                     /locus_tag="VW561_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU9943902.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
BASE COUNT         3881 a         2159 c         2820 g         3744 t
ORIGIN      
        1 gcaaggagaa taacgaaatt aagatatttt agatactcaa gattattgaa atcttagact
       61 aaaaaatagg tactgctacc tttaagaaga tagtagacgt atatactttt ttaagaaaat
      121 caaaaagata ctaaaaaaaa tctatttgtt tattgaattt aagactttgg taacaaattg
      181 aaaataaaaa ggaggtattc atcatgaata caaaaatgtt gtcacagtta gaggttatgg
      241 atactgaaat gcttgcgaaa gttgaagggg ggtatagctc cactgattgt caaaatgcac
      301 tgattacagg agtcactacg ggaattataa ctggtggaac tggagcaggt ttggccactt
      361 taggagtagc tggacttgct ggtgcatttg ttggagcaca cattggtgca attgggggtg
      421 gattaacatg cttaggaggt atggttggtg ataaattggg actaagttgg taagacttat
      481 agtaaaatta tgaggattct attctgattt attttagttt tttaaaaaag gagtatttgt
      541 catggataca aaaatgatgt cacaattttc tgttatggat actgaaatgc ttgcttgcgt
      601 tgaaggtggc ggatgcaatt ggggagattt tgccaaagca ggtgttggag gagcagctgt
      661 agttgctgct ttaggttgtg cagcgggtgg agtgaaatat gggaaaattc taggaccatg
      721 gggcgctgca ataggaggaa ttggaggagc agtggtttgt ggatatttag cctataccgc
      781 tacatcataa aaatgaaagc taaattttat gaaggctata ttctttatta ttctcttcgc
      841 ttttcaaacc tatcttattt atctatccat aagtatctct gataaaaaac aaaagactat
      901 tgaattaaca tcattgaatt gttttgtcat attatttcta atctatgata aattaatttt
      961 tctctttatt gcctatgttt ttttgataat ttttatatta aacttgttcc gtaactgaaa
     1021 agtgacatac tatatgaata caaaaatgat gtcacaattt tctgttatgg ataatgaaat
     1081 gcttgcttgc gttgaaggtg gagatattga ttggggaaga aaaattagtt gtgcagcagg
     1141 ggttgcatat ggcgcaattg atgggtgtgc aacaacggtt tgacatttct attgggacca
     1201 tttgctatag gaataggtgt aactggtgct gcaggtggag ctatacttgg aggtgtagcc
     1261 tatgcagcaa catgttggtg gtaattatgg attttaaaag ttttattatt ggtttagtag
     1321 ttggtatatt tggtccttat atggatgact taattagaaa aatattttca aaatcttcca
     1381 aaaaggatac ggatagtact ctctaaaaaa taaatttctc ccctgtattt gctagcttaa
     1441 tttacagggg agtttcttta atattgtttc gcctgtcaaa atgatatgtg atagcaggat
     1501 gaaaagtcta caaacaagtt ggtatcaata caatataaag aggaaagtat gtttttcatg
     1561 cttgcttttt taatttttac aattcaagag gttttgatga ccatttatga tttgagtgat
     1621 ccaaggtcaa aataagtgct atactagcag tgtaaatgtt cttgctcaac taagataaat
     1681 cgcacattta ggaggaaaac ttatgaagaa aaaaatactg attattttcg ttctgtatct
     1741 gatcatgtcc atctttcttt atccgcttag ggagagtatt tggtataatc tattttatac
     1801 catagcctat atgattgcgg ttatgatcta tttttcttta attaaaaaga aagaaaagaa
     1861 atgaaagatt ttcttgatta aaaagaatca tattctttgt gagattgttc ctgggtcagt
     1921 tgtctttgct tgtttctact tatctatttc tatttcgtca gttttccaat ttcttttagt
     1981 agttattaac ttgacttcta ttttggttac tgtttatctt actagggaaa tgagattaag
     2041 agagtttgaa gatgatgatt tggttagtcc tagaaccaat caactcatgt ttatcggctt
     2101 gacaggcttt atgtctatta tttgtttgta tagaggtatc acagcaggag aatcttatca
     2161 acaactaatc gcttatattg gtgctattct ttgcttgatc atcatgcttc tactcatttg
     2221 gggtttgaag tattataaaa agtaggggct aatgaagttt ttgcaattta ggaggtttcg
     2281 atgacaattc aagatttgga tgaaaaattt taaaaaacaa tgatatacta aacttgtcaa
     2341 agttgcaacg ggtgctgcta cattatgtta aaaatcgctc ttgattgata agttggattt
     2401 gttaggaaag gagtaaataa ttatggataa gaaaaagata gtttcgacta taatatgtat
     2461 agtatttctt gtagtgtcag tagataattt ttttagagac ttaaccccgt tactgttcat
     2521 tttaaatatt attggcctgt cttgcttttc agtgctaaca tacattaaca taaaagagat
     2581 actcttaaat ataagtaaat gatgatttgg ttagtcctag aaccaatcaa cttatgttta
     2641 tcggcttggc agattttatg tttgtcattt gtttgtatag aggtatttca gaaacagaat
     2701 tctatcaaca actaatcgct tatataggtg tattctctgc ttgtttatca cgcttctgct
     2761 catgtggggc ttgaagtatt ataaaaagta gctttctatc cttttctgac aggatatttg
     2821 agtagaaggc tctattaaat tgttagtgaa ataaatgaat ggaggtattt aatatgaaat
     2881 acagattatt ttttgttatt ttcttgagta gtgtgttgga tattctttta gggacatttt
     2941 tacaaatctc tatcgtatct attgggtggc ttgttcttta cagtggtttg tttgaagcag
     3001 gagttttcct tcttgctaat aaaggggtgg cggtaaaaat caaggaagta gatattcgaa
     3061 atcgctttaa atttattttt ggaaaaacct tatggtttca aattcttttg ctcatctttt
     3121 tgataatcaa actttatctt ggtttggatg cgaggttgat tttattctat ggacatattt
     3181 tcattgtctt taatgcctta atgtatctat tatctagtag tcaggttagc cttaaaaaaa
     3241 acaaactgtc ttcttaatct tacagcagta gagcctgaca aggtgaggtc atagtaggga
     3301 ggatttctat gaaaaagtat caacttctat tcaaaataag tgcagttttc tcttacttat
     3361 ttttcgtatt tggtctttct cagatgacgc ttattattca aaattattgg caattttctt
     3421 cccagattgg caatttcgtc tggattcaaa atttcttgag tttgctattt agcggagtca
     3481 tgatttggat tctggttaag acaggtcatg gttatctctt tcacattcca agaaaaaaat
     3541 ggctttggta ttcgattttg acagtattag tggtagtgct ccagatctct tttaacgttc
     3601 agacagctaa acatgttcag tcaactgctg aaggttgggc tgtattgatc ggttatagtg
     3661 ggaccaactt tgctgagcta ggtatctata taactttgtt ctttctgact ccacttatgg
     3721 aagagctaat ctatagagga ttactgcaac acgccttttt taagcattcg agatttggcc
     3781 ttgatttgct tcttccgtca attttatttg ctcttcctca tttttcaagc ctgcctagtt
     3841 tgttagatat cttcatcttt gcaacatctg gcatcatctt tgctagtttg acccgctata
     3901 ccaagagcat ttatccttcc tatgcggtgc atgtgatcaa taatattttc gcaacattac
     3961 catttttgct gactttttta catagggtct tggggtaaaa aaacaaaagg cttgcttttc
     4021 agccatagag gagattatca tgtataaaca cttatttttc ctagattcca aaactttaga
     4081 tcggttgaca ccctatattc tagtcttggc ttctgacacc attgctttta atgtttttgt
     4141 gctaaccttt gtatctgcgg tggtttttaa tttcctaaat tccatgctag ctttaatggc
     4201 tatattcata ggggctggct atgtggtcgg attttggtta ctaatactca atgaaaatca
     4261 aagagcaaac taggaaacta gccgcaggct gtacttgagt acggcaaggc gacgttgacg
     4321 tggtttgaat ttgattttcg aagagtataa aatggtttgt tttggaaagg ctagaactaa
     4381 agaatgacgc gtaggaagtc tgtttggtga ggaggatagt tttatggagt tttttgataa
     4441 atttcatgcc ttgtgttttg gatttttagt actaataatt gtcattacag ttccttatac
     4501 gattaaccat gggggttttt ttcaaaatga atctgcattg attcttgtaa gtcttcttgt
     4561 aacctcgctg agtgttgctt atgctagaaa gtttgaaatg atttcttttg ggatgttaag
     4621 caagaaacaa cttttgcttt tcattgcaat ctttcttcta agtgtacttg agacgctggt
     4681 ttatattcat ttcttcgctg tttcttctgg ctcaggggtc caacacttgg cggaagtcag
     4741 cagaggaatt tccctgtctt tgattttgac tacctcagtt tttggcccca tccaggagga
     4801 actcattttc agaggacttc ttcaaggtgc ggtttttgac aattcttggt tagggcttgt
     4861 gctaacttcc tctctctttt ctttcatgca tggaccttct aatgtccctt cgtttatttt
     4921 ttatctactt gggggtttgt tgctgggctt tgcttataaa aagagtcaaa acctatgggt
     4981 ttctactcta gtccacatgt tttacaacag ttggccactc ctatattatt tataaaaatt
     5041 atgaaaaggt aagcagaaga tggtgcttac ctttttcaag ctataatgat acccaagcca
     5101 atccagaggc ttttctttga gaatcgggtt agagtggttg acgaataggc caaaaactag
     5161 tagaatagta aggaaacttt atacggagga aagaaatgga tttgggtgat aatgagctaa
     5221 cactgactcc catacctggg aaaagtggca aggcttatat gggtagctat cctgacggga
     5281 agcgcatctt tgtaaaaatg aacacctctc caatcctacc tggtctagct agagaacaaa
     5341 ttgctccaca attattatgg agtcgtcgtt tggcagatgg gcgtgatatg tgtgctcaag
     5401 aatggttgac aggcaagata ttgaccccct atgatatgaa tcgtaagcaa atcgtcaata
     5461 ttttaacccg tcttcatcgc tcacgtccgt tgatgacaca attgagtcgt ttgggctatg
     5521 ccatggaaac acctgtagat ttactacagt cttggcaaga aacggcttca gatgctttgc
     5581 gtaaaaatca ttttatcagt gaagtgatgg ctgatttacg tcagactatt ccaggattta
     5641 gagaggacca tgcgaccatt gtccatggag atgtacgaca tagtaattgg attgagacag
     5701 atagtggctt gatttattta gtagattggg attcggttcg cttgaccgat cgcatgtttg
     5761 atgtggccca tatgctctgc cattatattt cagaacatca gtggaaggaa tggttgacct
     5821 actacggtta caagtacaat caaacggtat taagtaaatt gtattggtat ggtcaattgt
     5881 cttatttgag tcagatttcc aagtattata tgaaccaaga tttagaaaat gtcaatcggg
     5941 agattcatgg tttgcgtcat ttccgagaca agtatggaaa gagaagatga gagttagaaa
     6001 tcgtaaaggg gcaacagaat tactagaggc aaatccccag tatgtggtcc tcaatccctt
     6061 ggaagccaag gcaaaatggc gggacttgtt tggcaatgat aatcccattc atgtggaagt
     6121 tggaagtgga aagggtgcct ttgtttcagg tatggccaag caaaaccctg acatcaacta
     6181 tatcgggatt gatattcaaa agtctgtttt gagctacgct ttggacaagg tgcttgaagt
     6241 tggagtgcct aacattaaac tcttgtgggt agatggttct gacttgactg actactttga
     6301 agacgatgag attgatcgct tgtatctgaa cttttcagat ccatggccga aaaaacgcca
     6361 tgaaaagcgt cgtttgacct acaagacctt tttggacacc ttcaaacgta tcttgcctga
     6421 aaatggagaa attcatttca agacggataa ccgtggcttg tttgagtaca gtttagtgag
     6481 cttttctcaa tatggcatga aactcaatgg tgtctggcta gatttgcatg ccagtgattt
     6541 tgaaggcaat gtcatgacag aatacgagca aaaattctca aacaaggggc aagttatcta
     6601 ccgagttgag gcagaatttt aagagatagc ctaaaattag gctgtacaag tgcttttgct
     6661 ttacataagt tggcaaacgt gctatactga taataagaat atgaaaagtg aggcggggaa
     6721 atatcttcgc ctcttgctta tgaggaggtg gacgcaatcg caacaatcgt agaattagtc
     6781 agagaagttg tagaacctgt catagaagct ccttttgaac tcgtggatat cgagtatgga
     6841 aagattggca gtgacatgat tctcagtatt tttgtagata aacccgaagg aattaccttg
     6901 aacgacacgg cagacttgac agaaattatc agtcctgtcc tagacaccat caagccagat
     6961 cccttcccag aacaatattt cctagaaatt accagtccag gcttggaacg tcctttgaaa
     7021 accaaggatg ccgtcgctgg agcggttgga aaatacatcc atgtcggact ctaccaagcc
     7081 atcgataagc aaaaggtctt tgaaggaact ttgttggcct tcgaagagga cgagttgact
     7141 atggaatata tggacaagac gcgtaagaaa accgtccgaa ttccatacag tttagtatca
     7201 aaagcacgtt tagcagttaa attatagaaa aagaaaggat agcttttgag gattcaaaag
     7261 tgaagaaaac atgagtaaag aaatgctaga ggccttccgc attttggaag aagacaaggg
     7321 aatcaaaaaa gaagatatca tcgacgcagt agtagagtcg cttcgttccg cttatcgcag
     7381 acgctatggt cagtcagaca gcgtagctat tgacttcaac gaaaaaacag gtgactttac
     7441 agtttatact gtccgtgaag ttgttgatga agtatttgat agccgtttgg aaatcagctt
     7501 gaaagatgct cttgccatta attcagctta tgaacttgga gacaaaatca agtttgaaga
     7561 agcaccagct gagtttggtc gtgtagcagc ccaatctgcc aaacaaacca tcatggaaaa
     7621 aatgcgcaag caaacacgtg ccatcactta caatacttac aaagaacatg agcaagaaat
     7681 catgtctggt acagtagaac gctttgacaa ccgctttatc tatgtcaacc ttggtagcat
     7741 cgaagcccaa ttgtcaaaac aagatcaaat tcctggagaa gtttttgctt ctcatgatcg
     7801 tatcgaagtt tatgtttaca aggttgaaga caaccctcgt ggtgtgaacg tctttgttag
     7861 ccgtagccat ccagaaatga tcaaacgttt gatggagcaa gaaattccag aagtttatga
     7921 tggaactgtt gaaatcatga gcgtggctcg tgaagcaggt gaccgtacga aggttgctgt
     7981 tcgtagccac aatccaaacg tggatgctat cggtacaatc gttggacgtg gtggtgctaa
     8041 tatcaagaag attactagca aattccaccc agctcgttac gatgctaaaa atgaccgcat
     8101 ggtaccaatc gaagaaaata tcgatgttat cgagtgggta gcagatccag ctgaatttat
     8161 ctacaatgcc atcgctcctg ctgaggttga ccaagttatc tttgatgaaa acgacagcaa
     8221 acgtgccttg gtggttgttc cagataacaa gctttctctt gccattggtc gtcgtggaca
     8281 aaacgtgcgc ttggcggctc acttgactgg ttaccgtatc gatatcaagt ctgctagcga
     8341 atttgaagcc atggaagacg ctgcttcagt agagttggaa gtagaaaacg atactgtaga
     8401 agaataaaag ctgctagagg agggaaagat gaaaacaaga aaaatccctt tgcgcaagtc
     8461 tgttgtgtct aacgaagtga ttgataagcg tgatttgctc cgcattgtca agaacaagga
     8521 aggacaagtc tttattgatc ctacgggcaa ggccaatggc cgcggcgctt atatcaaact
     8581 agacaatgca gaagccctag aggcgaaaaa gaagaaggtc tttaaccgca gctttagcat
     8641 ggaagtggaa gaaagctttt atgacgagtt gatcgcttat gtggatcaca aagtgaaaag
     8701 aagagagttg ggacttgaat aagcaaaaga taagtaatct cttggggctt gctcagcgag
     8761 cagggcgcat catatcgggt gaagaattgg tggtcaaggc cattcaagac ggcaaggcca
     8821 agttggtctt tctagctcat gatgctggac ccaatctggc caagaagatt caagataaaa
     8881 gtcattatta tcaagtagaa attgtaaccg tgttttcaac actggaatta agcatagcag
     8941 tcgggaaatc gagaaaggtt ttggctgtaa cagatgctgg atttacaaag aaaatgaggt
     9001 ctcttatgga atagaagagg aggacatgat ttgtctaaga aaagattgta cgaaatcgca
     9061 aaagaacttg gaaaagaaag taaagaagtt gtagcgcgtg caaaagagtt gggcttggat
     9121 gtgaaaagcc actcatcaag tgtggaagaa gctgtcgctg caaaaattgc tgccagcttt
     9181 aagcctgcag ctgctccgaa agtagaagca aaacctgcag caccaaaagt aagtgcagaa
     9241 aagaaagccg aaaaatctga gccagctaaa ccagctgtag ctaaggaaga ggcaaaacct
     9301 gcagccccaa aagcaagtgc agaaaagaaa gccgaaaagt ctgaaccagt aaaaccagct
     9361 gtagccaagg aagaggcaaa accagctgag ccagtcactc cgaaaacaga aaaagtagcg
     9421 gctaaaccgc aaagtcgtaa tttcaaggct gagcgtgaag cacgtgctaa agagcaggca
     9481 gagcgacgca agcaaaataa gggcaataac cgtgaccaac aacaaaacgg aaaccgtcag
     9541 aaaaacgacg gccgtaatgg tggaaaacaa ggtcaaagca accgcgacaa tcgtcgcttt
     9601 aatgaccaag ctaagaagca gcaaggtcag caaaaacgta gaaatgagcg ccgtcagcaa
     9661 gaggataaac gttcaaatca agcggctcca cgtattgact ttaaagcccg tgcagcagcc
     9721 ctaaaagcag agcaaaatgc agagtacgct cgttcaagtg aggaacgctt caagcagtat
     9781 caggctgcta aagaagcctt ggctcaagct aacaaacgca aggaaccaga ggaaatcttt
     9841 gaagaagcgg ctaagttagc tgaacaagca cagcaagttc aagcagtggt tgaagtcgtc
     9901 cctgagaaaa aagaacctgc agtggataca cgtcgtaaaa aacaagctcg accagacaaa
     9961 aatcgtgacg attatgatca tgaagaagat ggtcctagaa aacaacaaaa gaatcgaagt
    10021 agtcaaaatc aagtgagaaa tcaaaagaat agtaactgga ataacaacaa aaagaacaaa
    10081 aaaggcaata acaagaacaa ccgtaatcag actccaaaac ctgttacgga gcgtaaattc
    10141 catgaattgc caacagaatt tgaatataca gatggtatga ccgttgcgga aatcgcaaaa
    10201 cgtatcaaac gtgaaccagc tgaaatcgtt aagaaacttt tcatgatggg tgtcatggcc
    10261 acacaaaacc aatccttgga tggggaaaca attgaactcc tcatggtgga ttacggtatc
    10321 gaagccaaac aaaaggttga agtggataat gctgacatcg aacgtttctt tgtcgaagat
    10381 ggttatctca atgaagatga attggttgag cgtccaccag ttgttactat catgggacac
    10441 gttgaccacg gtaaaacaac ccttttggat actcttcgta actcacgtgt tgcgacaggt
    10501 gaagcaggtg gtattactca gcatatcggt gcctaccaaa tcgtggaaaa tggtaagaag
    10561 attaccttcc ttgatacacc aggacacgcg gcctttacat caatgcgtgc gcgtggtgct
    10621 tctgttaccg atattacgat cttggtcgta gcggcagatg acggggttat gcctcagact
    10681 attgaagcca tcaaccactc aaaagcagct aacgttccaa tcatcgtagc cattaacaag
    10741 attgataaac caggtgctaa cccagaacgc gttatcggtg aattggcaga gcatggtgtg
    10801 atgtcaaccg cttggggtgg agattctgaa tttgttgaaa tctcagctaa attcaaccaa
    10861 aatatcgaag aattgttgga aacagtcctt cttgtagctg aaatccaaga actcaaggca
    10921 gacccaacag tgcgtgcaat cggtacggtt atcgaagcgc gcttggataa aggaaaaggt
    10981 gcggtcgcaa cccttcttgt acaacaaggt accttgaatg ttcaagaccc aatcgttgtc
    11041 ggaaatacct tcggtcgtgt ccgtgctatg accaacgacc ttggtcgtcg tgttaaagtt
    11101 gctggaccat caacaccagt ctctatcaca ggtttgaacg aagcaccgat ggcgggtgac
    11161 cactttgccg tttacgagga tgaaaaatct gcgcgtgcag caggtgaaga gcgtgccaaa
    11221 cgtgccctca tgaaacaacg tcaagctacc caacgtgtta gccttgaaaa cctctttgat
    11281 acccttaaag ctggggaact caaatctgtt aatgttatca tcaaggctga tgtacaaggt
    11341 tctgttgaag ccctttctgc ctcacttcaa aagattgacg tggaaggtgt caaagtgact
    11401 atcgtccact cagcggtcgg tgctatcaac gaatcagacg tgacccttgc cgaagcttca
    11461 aatgccttta tcgttggttt caacgtacgc cctacaccac aagctcgtca acaagcagaa
    11521 gctgacgatg tggaaatccg tcttcacagc attatctaca aggttatcga agagatggaa
    11581 gaagctatga aagggatgct tgatccagaa tttgaagaaa aagttattgg tgaagcggtt
    11641 atccgtgaaa ccttcaaggt gtctaaagtg ggaactatcg gtggatttat ggttatcaac
    11701 ggtaaggttg cccgtgactc taaagtccgt gttatccgtg atggtgtcgt tatctatgat
    11761 ggtgaactcg caagcttgaa acactataaa gatgacgtta aggaagttac aaacggccgt
    11821 gaaggtggat tgatgatcga cggctacaat gatattaaga tggatgatgt gattgaggcg
    11881 tatgtcatgg aagaaatcaa gagataagat tttttgctcc tttcttaggt ggtgagggac
    11941 gcaagcaaac cgatggtttc attgcttatt tttgagccta gggtctcaaa aatcccctgt
    12001 gatgggactg ataaatcagt tccatcactt tcaccacggc gaaagaagca gatgacttca
    12061 aattgaactt cgtttcaatt taaactgaaa atcaagaagt ttaaaatagc taggtctgct
    12121 ggcctagctt ttggttcaaa gtagagaaag gaatatcatg gcaaatcatt tccgtacaga
    12181 tcgtgtgggc atggaaatca agcgtgaagt caatgagatt ttgcaaaaga aagttcgtga
    12241 tccacgtgtc caaggtgtga ccatcacaga tgttcagatg ttgggtgact tgtctgttgc
    12301 caaggtttat tacaccattt tgagtaacct tgcttcggat aaccaaaaag cccaaatcgg
    12361 gcttgaaaaa gcaactggta ccatcaaacg tgaacttggt cgcaatttga aattgtacaa
    12421 aatcccagat ttgaccttcg tcaaagacga gtccatcgag tatggaaaca agattgacga
    12481 gatgctacgc aatctggata agaactaaag aagaggggtt gcccctcttt tttggtggag
    12541 gaaaataggt tgaatttgaa atggaaaaat attcttttaa aatatattga aactagaata
    12601 gtac
//
LOCUS       DAVKQL010000056        12253 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVKQL010000056 DAVKQL010000000
VERSION     DAVKQL010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12253
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.056"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(255..2513)
                     /locus_tag="VW561_001672"
     CDS             complement(255..2513)
                     /locus_tag="VW561_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9943903.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2742..2972)
                     /locus_tag="VW561_001673"
     CDS             complement(2742..2972)
                     /locus_tag="VW561_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEU9943904.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3162..3845
                     /locus_tag="VW561_001674"
     CDS             3162..3845
                     /locus_tag="VW561_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9943905.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSEIVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            3845..4579
                     /locus_tag="VW561_001675"
     CDS             3845..4579
                     /locus_tag="VW561_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9943906.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKVIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            4733..5590
                     /locus_tag="VW561_001676"
     CDS             4733..5590
                     /locus_tag="VW561_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEU9943907.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLAHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            5732..6604
                     /locus_tag="VW561_001677"
     CDS             5732..6604
                     /locus_tag="VW561_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEU9943908.1"
                     /translation="MKVINQILLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEPMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDASGENLVKQVFVNLSQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DALAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSKDKLV"
     gene            6886..7569
                     /gene="rpiA"
                     /locus_tag="VW561_001678"
     CDS             6886..7569
                     /gene="rpiA"
                     /locus_tag="VW561_001678"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEU9943909.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            7583..8794
                     /locus_tag="VW561_001679"
     CDS             7583..8794
                     /locus_tag="VW561_001679"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEU9943910.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEADIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            8796..9338
                     /locus_tag="VW561_001680"
     CDS             8796..9338
                     /locus_tag="VW561_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEU9943911.1"
                     /translation="MTRYALLVRGINVGSKNKVVMAELHQELTNLGLEKVESYINSGN
                     IFFASIDSKAQLVEKLETFFAVHYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYQKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            9354..10163
                     /locus_tag="VW561_001681"
     CDS             9354..10163
                     /locus_tag="VW561_001681"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU9943912.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIRFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKIDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            10639..11034
                     /locus_tag="VW561_001682"
     CDS             10639..11034
                     /locus_tag="VW561_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HEU9943913.1"
                     /translation="MLDSKEELKESENDAPKLETPLREEPRLAPQTLPEASEVLENKR
                     EESKVEITEPAQADDIRKVVGELAKDISITKLYMTGHSLGGYLAQIAAVEDYQKYPDF
                     YNHVLRKVTTFSAPKVITSRTVWDAKNGF"
     gene            11044..11619
                     /locus_tag="VW561_001683"
     CDS             11044..11619
                     /locus_tag="VW561_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEU9943914.1"
                     /translation="MESRKLAVSGKIKHYVVDNDNVVTPLIHNNRDIVTFTGNSRFKH
                     RSRGYFESPMNDIPNFNIGKQATLDKHGYRDPKLDKARFFKKQALPQSSSQPSAEPME
                     NIALGKQVTQSSTAFGGDARRAVDGKVDGNYGHNSVTHTNFQSKPWWQVDLAKEETIR
                     QINIYNRTDTAQDRLANFDVILLDSSGKEIE"
     gene            11644..12156
                     /locus_tag="VW561_001684"
     CDS             11644..12156
                     /locus_tag="VW561_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEU9943915.1"
                     /translation="MSAQITINHKKVRYVRIELEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGC
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
BASE COUNT         3619 a         2240 c         2774 g         3620 t
ORIGIN      
        1 tgcaatacct tttacgaggc tcttttgtcc tgctcttgtt tcaattgact atagcagatt
       61 gaaataagat gagaacaaat cgattgggaa agtaaaatta atttctataa atgttttagc
      121 aattgtttcg tactatttca gattcagtct actatatatc ataatggaca aagggcaagc
      181 tgtcaagaaa ttacagttat tttagtaaaa aaagaagcag aaaaatttca ttttctactc
      241 cttttctata ggatttattt ttctttttca gaacttttaa cttcagcttt tttggcagat
      301 ttaatctgaa tgtttccctt gctagtcata actgctttga gatctttttc gcttggattt
      361 tcaaggtagt agtcagtgat tgtgtcctca atatagtctt gaatagtccg acgaagtggg
      421 cgtgctccca tttttggatc ataacctagg tcaaccaact tttccttgac cttgtcagtt
      481 acatccaaac gaatgttgtt gctagagagg cgcttgttaa catctgctag catgagctcg
      541 acaatctgaa ggaggttatc cttgctgaga gccttaaatt cgataatgcc atcaaaacgg
      601 ttcataaact ctgggctaaa gaagttaccg agttcaccga ggacagaatt ggtacgtcct
      661 tctctagcag caccaaatcc aacgctagct tcggtctttc ctgtacctgc atttgaggtc
      721 atgataatga tggcatcctt gaagctaacg gtgcgtcctt gcccgtctgt caaacgacca
      781 tcgtccaaga cttgaagaaa catgtgcata acatctgggt gagctttttc cacttcatcg
      841 agaaggatga gagaatatgg attgtggcga actttttcag ttaattgacc agcctcatca
      901 tagccaacat aacctggagg agcgccgacc aacttagcca cactatgttt ttccatgtat
      961 tcactcatat caaagcgaat catactatca gcagaaccaa aaagttcgat agccagttgt
     1021 ttggaaagtt ctgtcttacc gacaccagtt ggcccaacga agaggaagct tccgattggg
     1081 cggttagggg taccaagtcc gacacgatta cggcgaatag ccttggcaat cttatcgact
     1141 gcatcatctt ggccaataac atgagacttg agatcttcgg ctagatggat gagttgagat
     1201 tgttctttct ctttcaaatc accaacaggg atattggttt tctgctcgat aatgtgctca
     1261 atagttttct cgctgatgat aggagtatcc tggtctgtga tctttttctt ttgcatttcc
     1321 ttatacttgg caatctggtc gcggaagtag gccgccttct caaaatcttc ttctcgtgta
     1381 gcttgagact tgagattttc agcctcaatc aagcgctgat caattacttt aggatccaca
     1441 aaattcaagg tcaagttcat cttagaacca gcttcatcta ggaggtcaat ggccttgtca
     1501 ggcaagaagc gatcttggat gtagcgattg gaaagagttg cagctgcttc aatcgcagca
     1561 tctgtatatt gaacgtggtg gtaatcttcg tatttctttt gaatcccttt gagaatagtg
     1621 attgtttcgt ccaccgttgg ttcatcgact ttaacaggct gcatacgacg ctcgagggca
     1681 gcatcctttt caatgatacg gtattcattg agggtagtag caccgactag ttgcagttct
     1741 ccacgagcaa gggctggctt gaggatattt cctgcgtcca tattaccatc actcgcagaa
     1801 ccagcaccaa caatttcatg gatttcatcg ataaagagga tgatgtcttc acgtttgcga
     1861 atttcttcca tgagtttttg catgcgttct tcaaattgtc ctcgaatccc cgttccttga
     1921 actaagctaa ccacatccag acggatgact tgtttacctt ggagtttatg tggcacatcg
     1981 ccatcgacaa ttttctgagc tagaccttcg acaacggccg tttttccgac accaggttca
     2041 ccgataagga caggattatt cttggttcta cgattgagaa tctcgatgac acggataatc
     2101 tcatcgtcgc gcccaataac ggggtcaatg tctccacgac gggcaatttc agttacatta
     2161 ataccaaatt cttccagcag gcctttttct tggctaggtg gcggagtttg agcagatcca
     2221 cgattttggg aaccataacc gccgtttcca ccgtaacctc cacctgattg ggttggggga
     2281 ataggaggag tattgctaga aggtctgaaa ttgtttagat cattgaagaa atcaccaaag
     2341 ggatcgaagt cacgattgtt cagatccgtc atacctttga agaggctatt gttaggatct
     2401 gttttgataa tcttatagca gttttgacag aggtcaattt gtttttgttt tccattgaga
     2461 ttggtgtaaa gatgaattgt tgagtcgtta attttacagt tttgacaaag catacatcta
     2521 cctcatttct ttctttagcc tgacttgttt aaaggtcaaa aatagtcaaa ttctatactc
     2581 tcattataac aggattgggg tgtaaagcaa gtaaaaagat tgaagctttg agaagttgtt
     2641 acaatgaaaa tttttgtgaa tttggactat aaaaaaatcc tatttgtgag acaaaaagga
     2701 ttttacttag acatgcaggg acaatcccag cttgttttag attagtagag aagttcgtaa
     2761 atttccattg cgattaagtc aatgctatca aacgtatacg tttcgcgatc ttctacgtca
     2821 cgaagggtaa agattgggcc gttttcttcg tcgtggctgt acgcaacttc tgcaacaaca
     2881 actccttcgc gttcaaaatt acgttttaaa tttccgccat cgtttgccat tgcttcaagg
     2941 cggttgatga ttctaaccaa atgtgattcc attttgattc tccttcattt cttatcgtta
     3001 ctattttacc ataaaggagg ccaacttgac tagaaaaaac taagatttct cgctaattct
     3061 gctagtttaa agatttttaa ataaattgga taaaaaattt aaattttaat tcaaaacatg
     3121 ttataatcat acagtattct attttagaaa gcagtgtgac tatgaatttt tcttttttac
     3181 ctaagtattt accttatttt aactatgggg ctgttgtgac gattcttatt tctatctgtg
     3241 ttatcttttt gggaactatt ttgggtgttg tcttggcttt tgggcaacgt tcaaagttta
     3301 aaccgcttgt ttggttggcc aacttgtacg tttggatttt ccgtgggaca ccgatgatgg
     3361 ttcaaattat gattgccttt gctcttatgc atatcaatgc tccgactatt cagattggaa
     3421 ttttaggtgt tgatttttcg cgtctgattc cagggatttt gattatctct atgaatagtg
     3481 gtgcttatgt ttcggagatt gttcgtgccg gaatcaatgc ggttccaaaa ggtcagctag
     3541 aggcggctta ttcgctaggg attcgtccta aaaatgcgat gcgttatgtg attttgccac
     3601 aagcagtcaa aaatatcttg ccagcattgg ggaacgaatt tatcaccatt atcaaggaca
     3661 gctccctctt atcagctatt ggggtcatgg agttgtggaa tggggctaca acagtttcta
     3721 caacaaccta tctaccttta acaccacttt tatttgcagc attttactac ttgattatga
     3781 cctctattct gacagtagcc ttgaaagctt ttgaaaaaca tatgggacaa ggagataaga
     3841 aataatgaca gaaaccttga taaaaattga aaatttacat aaatcttttg gaaagaatga
     3901 agtattgaag ggcatcaacc tcgagattaa aagaggagaa gttgtcgtta tcatcggtcc
     3961 ttcagggagt gggaaatcta ccttgcttcg ctctatgaat ttgttggagg aagcaaccaa
     4021 ggggaaggtt atctttgagg gagtcgatat tacggacaag aagaatgacc tgtttgccat
     4081 gcgtgagaag atgggcatgg ttttccaaca atttaacctc tttcctaata tgactgtgat
     4141 ggaaaatatt accttgtcac ctatcaagac taaaggtgaa agcagggaag ttgctgagaa
     4201 gagagcccaa gagcttttgg aaaaagttgg tttgccagat aaggcagacg cttatccaca
     4261 gagtctgtcg ggtgggcaac aacaacggat tgccatcgcg cgtgggttgg ctatggaacc
     4321 agatgttttg ctctttgacg aaccaacttc agccttggat cctgaaatgg taggtgaggt
     4381 attggctgtt atgcaagacc ttgccaagtc agggatgact atggttatcg taacacatga
     4441 gatgggattt gcccgtgagg tggcagatcg tgttatcttt atggcagacg gtgtggttgt
     4501 tgaagatggg acacctgagc agatttttga acaaacccaa gaacaacgga ctaaagattt
     4561 cttgagtaag gttttataat ctattttaaa atttcaagac aagattagtg aaaagttcaa
     4621 ccagagcttt ttcttatagt ttgaaactat aggataacct aggaaagaag tgttagaggg
     4681 cactagcagt ttttggtata atggaagata tttgaaagaa aagagaagtg atatgacaca
     4741 gattattgat gggaaagctt tagcggccaa attgcagggg cagttggctg aaaagactgc
     4801 aaaattaaag gaagaaacag ctctagtgcc tggtttggta gtgattttgg ttggggataa
     4861 tccagccagc caagtctacg ttcgcaacaa ggagaggtca gctcttgcgg ctggttcccg
     4921 tagcgaagta gtgcgagttc cagagaccat tactcaagag gaattgttag acctgattgc
     4981 taaatataat caggatccag cttggcatgg gattttggtc cagttgccat taccaaaaca
     5041 tatcgatgaa gaggcggttt tattagccat tgacccagaa aaggatgtgg atggtttcca
     5101 tcccctaaac atgggacgtc tttggtctgg acatccagtc atgattccct cgacacctgc
     5161 aggaattatg gaaatgtttc atgaatatgg gattgacttg gaaggtaaaa atgcggtcgt
     5221 aatcggtcgt tcaaatatcg ttggaaaacc tatggctcag cttcttttgg ctaaaaatgc
     5281 gacagtaacc ttggcccact cacgaactca taatcttgcc aaggtggctg ctaaagcaga
     5341 tattcttgta gtcgcaatcg gccgcgccaa gtttgtgact gctgactttg tcaaaccagg
     5401 tgcggttgtc attgacgttg ggatgaatcg agatgaaaat gggaagctct gtggagatgt
     5461 tgattatgag gcagttgcac cacttgctag ccatatcacg ccagtccctg gaggtgtcgg
     5521 tcctatgacc attaccatgc tgatggagca aacttatcaa gcagcacttc ggacattgga
     5581 tagaaaataa gagaaacatt cgattaatag agtgtatttt ctatagctat atctaaaatg
     5641 gcagaaatga atattaaatt ttagatataa gattacaaaa ggaggtctgc gtctcctttt
     5701 tattgtataa taaaagtgag aggaaaaaag gatgaaagtg attaatcaaa tcttattaga
     5761 aaaagttatt attgaacgtt ctcgttcaag tcataaagga gattatggtc gtctgctgct
     5821 gcttggtggt acttatcctt atggaggtgc catcatcatg gctgctttag cagctgtaaa
     5881 aagcggtgca ggattggtga ccgttggaac ggacagggaa aatatcccgg ctctgcacag
     5941 ccatttacct gagcctatgg ccttttctct tcaagaccag caattgttaa aagagcaatt
     6001 ggagaaggca gaagttgtct tgctggggcc tggtttacga gacgatgctt ctggagaaaa
     6061 tctagtaaaa caggtctttg ttaatttaag ccaaaatcag attttgattg tagacggagg
     6121 tgccttgacc attcttgcta ggacaagttt gtcatttccc tcaagccagc ttatcctaac
     6181 tcctcaccaa aaagaatggg aaaaattatc tggaattacg attgaaaagc aaaaggaaga
     6241 tgcaacggct agtgttctga cttccttccc tcaaggaaca attttggtgg agaaaggccc
     6301 agctactcgt atttgggaag ttggccaatc tgattattac cagttacagg ttggtggtcc
     6361 ttatcaggcg actgggggaa tgggagatgc tctggctggg atgatcgcag gatttgtagg
     6421 ccaattccga caggccagtc tctacgagcg tgtggcagta gcgacccatc ttcattcagc
     6481 catagcccaa gaactatctc aagaaaatta tgtggtctta ccgacggaaa tcagcaggta
     6541 tcttcccaaa ataatgaaaa ttatttgtca acaagagagg gtaagcaaag ataaacttgt
     6601 ttaaaataga cagataaatc tacttccttc tcttaatgta tgattcttga aatgcgcctg
     6661 ctttaaggct aaactagacg tatcaaagtt aagagtttgt ttttgtaact ggaaagagat
     6721 gaacaaatca aatacataaa agataaactc atttttattc gtttggtaaa aataaaatgc
     6781 atatttttaa agaaagattg atgaaaaatg tagatttgat gtttttactt gaataaaaag
     6841 cggtttcata gtaaaataag aactgagaat gatggaggta aatcggtgga aaatctgaag
     6901 aaaatggcag gtatcaaggc tgctgagttc gtgagcgatg gaatggtcgt tggacttgga
     6961 acaggctcga ctgcctatta ttttgtcgaa gaaatcggtc gtcgtatcaa ggaagaagga
     7021 ttacagatta cagctgtaac aacttctagt gtgaccacta aacaggcaga aggccttaat
     7081 atcccgctca agtctattga ccaagtagac tttgtcgatg tgacggttga tggcgcggat
     7141 gaagtggata gtcagtttaa tggaatcaaa ggcggtggtg gtgcccttct catggaaaag
     7201 gtggtcgcaa caccatcaaa agaatacatt tgggtggtgg atgaaagcaa gctggtcgaa
     7261 aaactaggtg cttttaaatt gccagtagaa gtggttcagt atggtgcaga gcaggtcttt
     7321 cgtcattttg aacgagctgg ctacaaacca agtttccgtg aaaaagacgg ccaacgtttt
     7381 gtgacggata tgcagaattt tatcattgac ctcgccttgg atgtcattga aaatccaatt
     7441 gcttttggac aagaattgga ccatgtcgtt ggtgttgtgg agcatggttt attcaaccaa
     7501 atggtggata aggtaatcgt tgctggacga gatggagttc agatttcaac ttcaaaaaaa
     7561 ggaaaataga agggggcata agatgtctaa atttaatcgt attcatttgg tggtactgga
     7621 ttctgtagga atcggtgcag caccagatgc taataacttt gtcaatgcag gggttccaga
     7681 tggagcttct gacacactgg gacacatttc aaaaacagtt ggtttgaatg tcccaaacat
     7741 ggctaaaata ggtcttggaa atattcctcg tgaaactcct cttaagactg tagcagctga
     7801 aagcaatcca actggatatg caacaaaatt agaggaagta tctcttggta aggatactat
     7861 gactggacac tgggaaatca tgggactcaa cattactgag cctttcgata ctttctggaa
     7921 cggattccca gaagaaatcc tgacaaaaat tgaagaattc tcaggacgca aggttattcg
     7981 tgaagccaac aaaccttatt caggaacggc tgttatcgat gattttggac cacgtcagat
     8041 ggaaactgga gagttgatta tctatacttc agctgaccct gttttgcaga ttgctgccca
     8101 cgaagacatt attcctttgg atgaattgta ccgtatctgt gaatacgctc gttcgattac
     8161 ccttgagcgt cctgcccttc ttggtcgcat cattgctcgc ccttatgtag gtgaaccagg
     8221 taacttcact cgtacggcaa accgtcgtga cttggctgta tctccatttt ccccaactgt
     8281 tttggataaa ttgaatgagg ctgatatcga tacttatgct gtgggtaaaa tcaacgatat
     8341 ctttaacggt gctggtatca accatgacat gggtcacaac aagtcaaata gtcatggaat
     8401 tgatacacta ttgaagacta tgggacttgc tgagtttgaa aaaggattct cattcacaaa
     8461 cctagttgac tttgatgccc tttacggcca tcgtcgtaat gctcacggtt accgtgattg
     8521 cttgcatgag tttgatgaac gcttacctga aattatcgca gctatgagag agaatgacct
     8581 tctcttgatt actgcggacc atggaaatga cccaacgtat gcaggaacgg atcacactcg
     8641 ggaatatatt ccattgttgg cctatagccc tgcctttaaa ggaaatggtc tcattccagt
     8701 aggacatttt gcagatattt cagcgactgt tgccgataac tttggtgtgg aaactgctat
     8761 gattggggaa agtttcttag ataaattggt ataagatgac gcgctatgct ttgctggtga
     8821 gaggtatcaa tgttggtagt aagaataagg tcgtcatggc ggagcttcat caagaattga
     8881 caaacttggg actggaaaag gttgagagct acatcaacag tggcaatatt ttctttgctt
     8941 cgatagattc caaagcccaa ttggttgaaa agctagagac tttctttgca gtccattatc
     9001 catttattca gagcttttct ttactgagtc tagaggactt tgaggcggaa cttgaaaatc
     9061 taccagcttg gtggagcaga gacttggcac gaaaagattt tctcttttac actgagggtt
     9121 tggatgtgga ccaagtcatc gcgacagttg aaagtttaga gctgaaagat gaagtgcttt
     9181 attttggaaa acttgggatt ttctggggga aattttctga agaatcctat tctaagactg
     9241 cctatcagaa gtacttgctg aaggtgcctt tctaccgcca cattactatt cgtaatgcta
     9301 aaacctttga caaaattggt caaatgctaa aaaaataata aaggagacac acaatgacat
     9361 ttttaaacaa aatccatgaa actgctactt tcctgaaaga aaagggaatt gcagcccctg
     9421 agttcggtct aatccttggg tcaggacttg gagaattggc agaagaaatc gaaaatccag
     9481 ttgtagtaga ctatgctgag attccaaact ggggccgttc aacagtagtc ggtcatgctg
     9541 gtaaattggt atatggtgaa ctggcaggtc gcaaggtctt ggctcttcaa gggcgtttcc
     9601 atttctatga agggaatcct ctggaagtgg tgactttccc agttcgtgtg atgaaagttc
     9661 ttggatgtga aggtgttatt gtaaccaatg cagctggcgg tatcagattt ggtcctggta
     9721 ccttgatggc tatctcagac catatcaaca tgacggggca aaatccattg atgggtgaaa
     9781 acttggatga ctttggccca cgtttcccag atatgtctag ggcctacaca ccagaatacc
     9841 gtgccactgc ccatgaagtg gctaaaaaac ttaatatcaa gattgatgaa ggtgtctata
     9901 tcggagttac tggtccgact tatgaaacac cagcagaaat tcgttcctat aagacactgg
     9961 gagcagatgc agttggtatg tctacggttc ctgaagttat cgtggcagcc cactctggct
    10021 tgaaagttct gggaatttca tgtatcacta actttgcggc tggtttccaa gaagaactca
    10081 atcacgaaga agttgtagaa gtgactgaac gtgttaaagg tgatttcaaa ggcttgctta
    10141 aagcgattct tgctgaattg taagaaaaaa gatttaaaag ggggagtgcc tctgtctttt
    10201 caggattgac tgcctatccg tattaaagaa gaaacagagg aatactatga gcttcttcct
    10261 gctcttataa ctgaaagaag cggaagaata ggtgtgtctg atctgatagc cagcattgtg
    10321 aaagacaaga ttctaggata ctagcattag cttcctagcc aagcagacta gtatgataag
    10381 gagagatgag aatgaattga ctttctgaat ttctcagtct tatcatatat agcacaatga
    10441 gatttcgctt gagtctgctt gtaaataaac gaaaagaaag ataagaaata atgaaaattg
    10501 gtcaacgaat tatgcgcttt ggcataaaaa attaagtatc ggagttgtat ctgttgtagt
    10561 cggctttgat ttctagctcc agctggaatt tcagccaatg aagtaaagca agatgtaaca
    10621 tctgaagtgg taataggtgt gctagattct aaggaggaat tgaaagagtc agaaaatgat
    10681 gctccaaaac tagaaactcc tcttagagag gagccaagac tagctcctca aacgcttccg
    10741 gaagcaagtg aagttcttga aaacaaaagg gaagagtcaa aagtagagat aacagagcca
    10801 gctcaagcgg atgatatccg caaggttgtt ggggaattag ccaaggatat aagtattact
    10861 aagttgtata tgacaggtca ttctcttgga ggctacctag ctcagattgc agcggttgaa
    10921 gattaccaaa aatatcctga tttttataac catgtattga ggaaagtgac aactttcagt
    10981 gctcctaaag tgattacttc cagaactgtt tgggatgcta agaatggttt ctgagatgtt
    11041 ggtttggaaa gtcgtaaatt agctgttagt ggaaaaatta agcattatgt ggttgataat
    11101 gacaatgttg tgactccctt gattcataat aatcgtgata ttgttacatt tacaggtaat
    11161 tcacgcttta aacaccgttc tcgtggctat tttgaaagtc caatgaatga tattcctaac
    11221 tttaatattg gtaaacaagc taccttggat aaacatggtt atcgtgatcc gaaattggat
    11281 aaagcgcgat tctttaagaa acaggctcta cctcaatctt ctagtcaacc aagcgctgaa
    11341 ccaatggaaa atattgcctt aggaaaacag gttactcaaa gttcgacagc tttcggagga
    11401 gatgctagaa gagctgtgga tggcaaagtc gatggtaact atggtcacaa ttctgtcact
    11461 catacaaact tccaatctaa gccttggtgg caagtagatt tggctaaaga agaaaccatt
    11521 cgccaaatca atatttacaa ccgaacagac actgcccagg atagattggc aaactttgat
    11581 gtcattcttt tagacagttc tggtaaagaa attgagtgaa aacgtataac atctcctaaa
    11641 gatgtgtcag cacaaattac gattaaccat aaaaaagtgc gctatgttcg gattgagcta
    11701 gaaggctata atgccctcag tcttgcagaa gttgaagttt tccgctttat agctacgaat
    11761 gctgaaacgg cgacacaagt ttctaagcca gttcaaccaa tcagtcagac tcctgtgaaa
    11821 gataaaacat tgacaattca acatagtgga gcttacattg cccgctactc cataacttgg
    11881 gaagaagttc cagtagataa agatggaaac caagttgttc gtagtcattc ttgggaagga
    11941 aacggttgca accagactgc aggttttgtc ctcaacctcc caatcaaaga aaatatgaga
    12001 aatctgcgag ttaagattga gaaaaagacg ggcctactat ggaatagatg gcaaacaatc
    12061 tatgaaaaca gaccaatttt agctcaaccc caccgtaaaa ttacccattg gggtacgaca
    12121 ttgaattcca aggtgagtga cgatgatgtc ttgtaatctg atggtacaat gacagttagt
    12181 ttgtctagtt tataagaaag tactacctga gcttgaatag aactcaggta gctctctgtg
    12241 aaagaacaaa att
//
LOCUS       DAVKQL010000057        11972 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVKQL010000057 DAVKQL010000000
VERSION     DAVKQL010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11972
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.057"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            33..251
                     /locus_tag="VW561_001685"
     CDS             33..251
                     /locus_tag="VW561_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9943916.1"
                     /translation="MEQITLKGARVSAGYTLREVGLKINRSFQTVAKYEKDSTKIPID
                     LLRELTSLYNISPDLIFLGKSTKKNELI"
     gene            265..891
                     /locus_tag="VW561_001686"
     CDS             265..891
                     /locus_tag="VW561_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEU9943917.1"
                     /translation="MELVYMDGKKEPYTLSSIIAECAEVKHRHLKILLNKHREDFESF
                     GKVQFKISPSESGQNVRDYILNEQQATLLITYLRNTEPVKEFKKNLVKAFFEMREELS
                     KFRMQRALEKPKRKTLHDSIENWKQAPKHAHSTMNNLLLKAVTDRNAKQLREERGGYN
                     GIDSLTSDELEQYQAFEDMVIAMISLNMSYQEIKAMAFRNKKTRQRSA"
     gene            1326..1778
                     /locus_tag="VW561_001687"
     CDS             1326..1778
                     /locus_tag="VW561_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943918.1"
                     /translation="MTETTYDIIAKSLDRISMELHQADENNDFLRIGLLSGQLKAIKE
                     NLHRLLWIELPELNESHKIEAVSKSTTGMYFHPGIFEMDAMRQAFFKRQAKHFFDNEA
                     EQQAYIEHAEKEYLEATITLKDILFNFKNANRKVSKDHLIEKFEEAMK"
     gene            1775..1990
                     /locus_tag="VW561_001688"
     CDS             1775..1990
                     /locus_tag="VW561_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943919.1"
                     /translation="MNELDLTNTQALIFMVVLIGLLLYLNHRDRKKSAKIERESQQTI
                     ETPSEDLNPYYGRYIQLAGVRVYGGTE"
     gene            1987..2328
                     /locus_tag="VW561_001689"
     CDS             1987..2328
                     /locus_tag="VW561_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9943920.1"
                     /translation="MSLSENDKRVLRLIKVGAENSITGAEISLITKLAERTVQDIISR
                     LITRNSIPIVGVRHGTFRGYFIPANKEELLDSAKTFYNQIREEEKRLVVLMNSDLESY
                     KETLKEVGGYV"
     gene            2321..2602
                     /locus_tag="VW561_001690"
     CDS             2321..2602
                     /locus_tag="VW561_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEU9943921.1"
                     /translation="MFSLSRESEQDLAHGIIEVVERYLEARDTVKPRLTGLISAQEVM
                     GELDIKYLTLQKWEKAGLRRYHPPLEDTRKVYYKITDIWKFMGVENGKN"
     gene            2589..3386
                     /locus_tag="VW561_001691"
     CDS             2589..3386
                     /locus_tag="VW561_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEU9943922.1"
                     /translation="MAKTKIYFWLKIDKKFFDNIFIKRLKTVPGGYTMTVIYIRLMLE
                     SLESDCILYYEGYFDNLKEELALKLDVSEDDIDMTMAYFTKCGLIQVDEDKNAELPQA
                     KALVQQETNQAAYMREYRKQQREQKENLTMSSENLTLLTTCKTEIEKEIEKELEEEIE
                     KKTSSAPTAEISNYYQKRIGVMDGQQYQILTDYLTLDGMELEVIKIAIDKAADNGKRS
                     FSYINSILKNWRQNGIRTMVQVEDEQRLFQQKKQGQSDDDIQDPFIY"
     gene            3402..4226
                     /locus_tag="VW561_001692"
     CDS             3402..4226
                     /locus_tag="VW561_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9943923.1"
                     /translation="MELLNTDQLNEKIKVTDELCPIHQINLVQFKKPDGYTSPYCMEC
                     TRENIKQTELEGIEKALGHDLDSSTYDVLVRESTVSNELREATFSTFKAETQEEHEAK
                     EFAIKQAKQYLNGMTGNTLIMGNPGTGKSHLCYSMAKAINEGYKSRNDPKSVLFVSIT
                     EIITRIQSGWQYRQSDFTEYDALKLLTEVDYLFIDDLGTESVMNSQKNEANNWVQAFL
                     FKIFDKRDTTIINTNHNGKELARIYNDKLVSRIGKQSEGNVFIITDIKDKRMKRNF"
     gene            4358..4891
                     /locus_tag="VW561_001693"
     CDS             4358..4891
                     /locus_tag="VW561_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943924.1"
                     /translation="MTLKTFSDKAKTFTFTYEFKDLDTALVAGHALLGYMTGTYCQPT
                     ISLTHKDKGTLVAEYVEDNKLNKTFKRICDSFKDYYNQPVDDEAFEERYKRERVLQLK
                     ESEDFDSLLNKVTDYELELLDYADRLLSDKPIPMDSMTAFGTLEMLGNESINLLQNLD
                     VEGEYKGLADYVAEPES"
     gene            6103..6522
                     /locus_tag="VW561_001694"
     CDS             6103..6522
                     /locus_tag="VW561_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEU9943925.1"
                     /translation="MTPEQVKEKLEGVKWINKEIKGLYLELEALEGGIIQKPTLSHSR
                     VQTSRENKTENNFISVLKLKEDTLQRIERLTEERMEISGLIDKLANPLERSVLRLFYL
                     NDLDIWGVAEEIGKSKASIYRAKKAAIEHLVCMVNGN"
     gene            7271..7480
                     /locus_tag="VW561_001695"
     CDS             7271..7480
                     /locus_tag="VW561_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943926.1"
                     /translation="MAKVKEKWNPTISHIVPKGTKLSDGTILDKETTLTQEEFTKNPP
                     VIPAGHPYYNLLARISREEIEKEDL"
     gene            8032..10863
                     /gene="uvrA"
                     /locus_tag="VW561_001696"
     CDS             8032..10863
                     /gene="uvrA"
                     /locus_tag="VW561_001696"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU9943927.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLHEGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVTGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            10856..11917
                     /locus_tag="VW561_001697"
     CDS             10856..11917
                     /locus_tag="VW561_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU9943928.1"
                     /translation="MNKRVQAFLAKIQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
BASE COUNT         3956 a         2237 c         2686 g         3093 t
ORIGIN      
        1 tcaattatca ttttagaaag gattttttaa agatggaaca gattacttta aaaggagcgc
       61 gagtttctgc tggttatacg ttgcgtgaag ttgggttaaa aattaataga agttttcaga
      121 ctgtcgcaaa gtacgaaaaa gatagtacta aaatacctat tgatttacta agagaattaa
      181 cttcacttta taatatttcc cctgatctta tttttttagg aaaaagtacg aaaaaaaacg
      241 aacttattta gaaaggagca aacaatggaa ctagtctaca tggacggaaa gaaagagccg
      301 tatacactaa gcagtattat tgcggagtgt gcagaagtca agcacagaca tttgaagatt
      361 ttgctaaata aacaccgaga ggacttcgag agtttcggaa aggtgcaatt taaaatttca
      421 ccttcagaaa gtgggcaaaa tgtacgggac tatattttaa atgaacaaca agcaacattg
      481 ctgatcactt acttacgaaa tacagaaccc gtaaaagagt ttaagaagaa cctagtcaaa
      541 gccttctttg aaatgcgtga ggaactttct aagttccgta tgcagagggc gctagaaaag
      601 ccaaaaagaa aaacactgca tgacagtatt gagaactgga aacaagcacc aaagcacgcg
      661 catagcacca tgaacaacct gctactaaaa gcagtaaccg acaggaacgc taagcagtta
      721 agggaagaac gtgggggcta taatggtatt gatagcttga caagtgacga gctggagcaa
      781 tatcaagcct ttgaggatat ggtaatagct atgattagct tgaatatgag ttatcaggaa
      841 atcaaggcta tggcattcag aaataaaaaa acacgccaaa gaagcgcgtg agagcaacaa
      901 aaaaggctta gcaataacca caccgcagag cctttcacac taacactaaa acgaatttaa
      961 caaagcaggc aagctattat caaagaggtt ttagtaaaga tttataccta gattatagca
     1021 tatttagggc aatttgacca tacggagagc gccaactctt taaactggta ctttctcacg
     1081 ctttcaattt ggcgactctg agcgtgaggc atgtggtaag aaaaaaagca ttaaaaagcc
     1141 ccacgctctc ggtcggcaaa cttctgagca tgaggctgta ggcaagaaaa actgtataag
     1201 aaacaaccat tcaaaaaacc cacactcgcc atcgcccaac tttgagtgtg aggatatcct
     1261 ggatagtaaa aggcattaaa aagcccttag ggcaattaca caacatagaa acataggtaa
     1321 aaaacatgac agaaacaaca tacgatatta tcgctaaaag cttggataga attagtatgg
     1381 aattacatca agcagacgaa aacaatgatt ttttaagaat aggactctta tcaggacaat
     1441 taaaagctat caaagaaaat ttacaccgct tactttggat tgaacttcct gaattgaatg
     1501 aaagccataa aatcgaggca gtctctaaaa gcactacggg aatgtatttc caccctggta
     1561 tttttgaaat ggacgctatg cgacaagcct tctttaaacg ccaagccaag cacttttttg
     1621 acaacgaagc agagcaacag gcgtatatag aacatgctga aaaggagtat ttagaggcta
     1681 ctataacctt aaaagatatt ctttttaatt ttaaaaatgc taataggaaa gtaagtaaag
     1741 atcatcttat agagaagttt gaggaggcga tgaaatgaat gagctagatt taaccaatac
     1801 acaggcgcta atcttcatgg tggtactgat tggcttactg ctttatctaa accaccgaga
     1861 ccgcaaaaaa agcgccaaaa tagagcgaga aagccaacag acgatagaaa cacctagcga
     1921 ggatttaaac ccttattatg ggcgttatat ccagcttgca ggggtaagag tttacggagg
     1981 aactgaatga gtctatcaga gaatgacaaa cgagtattaa gactaatcaa ggtaggggct
     2041 gagaactcca taacaggggc agaaatcagc ctgatcacta agctggcaga aagaaccgta
     2101 caggacatta tcagccgtct aatcacgcgc aatagcattc ctattgttgg ggttcgacac
     2161 gggacattta gaggatattt tatcccagct aacaaagagg agctattgga cagtgcaaaa
     2221 accttttaca atcagatacg agaagaagaa aagcgcctag tggtgttgat gaacagcgac
     2281 ctagaaagct acaaagaaac cttgaaggag gtgggcggat atgtttagcc taagccgaga
     2341 aagcgagcaa gacctagcgc atggcattat agaggtagtg gaaagatacc tggaagcgcg
     2401 tgatacagtc aaaccacgac tgacaggttt aatctcagcc caggaagtca tgggcgagtt
     2461 agatataaaa tatctgacac tccaaaagtg ggagaaagca gggctaaggc gataccatcc
     2521 accgcttgaa gatacaagaa aggtttatta taaaataact gacatctgga agtttatggg
     2581 ggtggagaat ggcaaaaact aaaatatatt tttggctaaa aattgataag aaattttttg
     2641 ataatatttt tatcaagaga ctaaaaacgg tacctggtgg gtacactatg acggtgattt
     2701 atattaggct catgctagag agcttagaaa gtgactgtat tttatactat gagggttact
     2761 ttgacaatct caaggaagag ttagccctaa aattagatgt atcggaagat gatatagata
     2821 tgaccatggc atactttact aagtgtggtc taatacaagt tgatgaagat aaaaacgcag
     2881 aactaccgca ggcaaaagcc ttggtacagc aggaaaccaa ccaagcagca tacatgcgag
     2941 aatataggaa acagcaacga gaacaaaagg aaaaccttac aatgtcatct gagaatctta
     3001 cattgttaac tacatgtaag acagagatag agaaagagat agagaaagag ttagaagaag
     3061 agatagagaa aaaaacttct tctgctccta ctgctgaaat ctctaactat taccaaaagc
     3121 gtataggggt tatggatggg caacaatatc aaatactgac tgactatctc actcttgacg
     3181 gtatggagct agaggttatc aagattgcta tagacaaggc tgcagataat ggcaagcgat
     3241 cttttagcta tatcaattca atactcaaga actggcgaca gaatggtatt agaaccatgg
     3301 tacaagtcga agatgaacag agactgtttc agcaaaagaa acaaggtcag tctgacgatg
     3361 atattcaaga cccgtttatc tactgacgga gaggagaaac aatggaatta ctgaatacag
     3421 accagctcaa tgaaaaaata aaggtcactg atgagctttg cccaatccat cagattaacc
     3481 tagtacaatt caaaaagcca gatggttata catctcccta ctgtatggaa tgcactaggg
     3541 agaacatcaa acagacggag ctagagggca tagaaaaagc cctaggccat gatctggact
     3601 cttcaaccta tgatgtgcta gtgcgtgaaa gtacggtgtc aaatgagctg agagaagcca
     3661 cgtttagcac tttcaaagcc gagacgcaag aggaacacga ggccaaggag tttgctatca
     3721 agcaagctaa gcagtaccta aacggtatga caggaaacac gctgattatg ggcaatccag
     3781 gtactggcaa aagtcattta tgctattcta tggctaaagc cattaacgag ggctataagt
     3841 ctaggaatga tcctaagagt gtgctttttg tcagtattac tgagattatc acgcgcattc
     3901 agtcaggttg gcagtataga cagagtgatt ttacagagta tgacgctttg aagttgctga
     3961 ctgaggttga ctatctcttt attgatgatt taggaacaga aagcgttatg aacagccaaa
     4021 aaaatgaagc taataattgg gtacaggcct ttcttttcaa gatttttgat aagcgagaca
     4081 caactatcat caacaccaat cacaacggaa aagagctggc cagaatctat aatgataagc
     4141 tagtcagtcg cattggaaaa caatcagagg ggaacgtatt tattattaca gacattaaag
     4201 ataagaggat gaagcgcaat ttttagccgg agaaaaagga gaaaaacaac atgaaagtca
     4261 agctaaacga cttcatggca actgttgaag tgctggacgt taaattttct ggtacaccat
     4321 ctagtgacac ccttaaaaca gaaagaggaa aaacaacatg acattaaaaa cattttcgga
     4381 taaagccaaa acatttactt tcacttacga atttaaagac ctagatactg ctctggtagc
     4441 aggtcacgcg ctactgggat atatgactgg aacatattgc cagcctacaa tctcattgac
     4501 ccacaaggat aaaggcactc ttgtagctga gtatgtggag gataacaaac taaacaagac
     4561 cttcaagcgt atttgtgaca gttttaaaga ctactacaac caaccagtgg acgatgaagc
     4621 atttgaagaa cgttacaaac gggagcgcgt gcttcaactc aaagagtcag aggattttga
     4681 tagtttgctg aataaggtca cagactacga gctggaacta ttagactatg ctgatcgctt
     4741 gctttctgat aagcctatcc ctatggactc tatgaccgct tttggtactt tggaaatgct
     4801 gggtaatgaa agtattaacc tgctccaaaa cttggacgta gaaggcgaat ataaaggcct
     4861 tgctgattat gtggcagagc cagaaagcta gaactatcaa gagaggcact agcctctttt
     4921 tgtggtttca ttgctaccta acccaatcga tttgggtttt ttacatgata atttgtagta
     4981 gtgtgcaatt tacaaaacaa acaaatacta catatttgac ggtttgaatg ttaaaaaagc
     5041 tagatatatc aaggatttaa gtaagtagag tgttccaaaa aagggtgcag taagcgttcc
     5101 aaaaaaaggg tgagataagg gtgaggttga atatataaaa agtataagat tagtataagg
     5161 ttgtgtattt agaaatacaa agtataggct aagattaggc taaggtcaca tacttcaaaa
     5221 tactaaggtt ttacaaaggt acagggcttt ttttctagtg atagaacccc aaggttacca
     5281 caaggtatag ctactttttt agaaccatag aacggtaagg ttacggtaag gtatcaaagt
     5341 tttccaatgg tgaggttttg gtgaggtaag agcaaaaaat gtaaaggttt tgtaaaggtt
     5401 gcgtatttga aagccccaag gttaccacaa ggtagagagg tttaccgagg gttagaaaag
     5461 gtgagatttt gcttaggtag tggcagtaaa tatgcctgac aaatggcagg gttatgtgtg
     5521 ttgaactccg agcgaacgcc gagaaagtgg atatatcccc tatcatttat caagcagata
     5581 agagaaataa taaccctcct taactccctg agaaacgaca agcagataag cggagttttt
     5641 acccctctta tttccgattt actccttgca gaatgatagg cagataagga cgacaataag
     5701 ccttcttaat tgcttacaaa acgataggga gttaagacga atttaaaagc tctttatttt
     5761 cgtttttctc cttacaaaat gacagggaaa taagaactaa aataactctc cttattttcc
     5821 taccaaatga taaggagata agagcaattt aaaagtctgt tattttggat ttgttgctta
     5881 ccaaatgata agtagatcag caaaggaata acttgcctta attgcttgca gaatgacaag
     5941 gaaataagag agataaaaac atcccttaat tgcttatcaa atggtacgta gaatggactt
     6001 gaacctagag cgaagctaga gaaaatgaaa aaaagccagc acacggctga ctcctttatg
     6061 aattattccc taaaactatt atatcacaaa ggaggccgag gcatgacacc agagcaggta
     6121 aaagaaaaac tagagggcgt taagtggata aataaggaga tcaaaggctt atatttggaa
     6181 ttggaagccc tggaaggtgg cattatccaa aagccaacac taagccatag cagggtgcag
     6241 acaagcagag agaacaagac agagaacaat tttataagtg ttctgaagct aaaagaggat
     6301 acgcttcaga ggattgagcg gcttactgag gaaagaatgg aaatatctgg gctgatcgat
     6361 aagctagcca atccgcttga gcgctctgtt ctgagacttt tttacttgaa tgatctcgat
     6421 atttggggag ttgctgagga aataggtaaa tctaaagctt cgatatatcg ggcaaagaag
     6481 gcagctatag aacacttggt atgtatggta aatgggaatt gatttaacct taccacagag
     6541 cctagtgcgt atagggggca ctaggcttta tattcgcccc ttattcgttt tgttttggac
     6601 tggataacta aatcaccacg gataaactac aagccctcag aaacaaatct aggggctttt
     6661 gtgagataaa aaaataaagg tgccccaccg cctccacagc aagctgcttc atgggcgtta
     6721 gaccaccttt accttaactt aattataact cacaatgcgg agtttttcaa gaaaggataa
     6781 gagactagaa aactagctac ttattggaac aacaaagcta gtgagctatt gaagaacggt
     6841 aagattttgg ttagatacag agaaacaggc ctaagcctgc tctaaatcta acttaatttc
     6901 ttcacaaact attaaattca aataagctga gaaaacataa agggtaacaa aaaggggact
     6961 taaattgaag agttcagtaa gcaagaaact aacacaatca aacgtgcttt tttttgtaaa
     7021 aatgttgtat acttaagtaa ataaaggaac tggtttggca cccaatggct tattttaggt
     7081 cggcgcctaa gtcagttcct ttttactgtt tcgaccaccc caaaggatat gcctctaaca
     7141 ttattgcgtc aattagttat ttaggctata attaaattaa cgaatagagg ttttccatct
     7201 ccccttgaaa cagttcattc cgcggtagga ggtgggacgc ctctattttt accataaagg
     7261 agtatttaac atggcaaaag ttaaagaaaa atggaaccca acaatatccc atattgtccc
     7321 taaaggtacc aaactatcag acggtacaat attagacaaa gaaaccacct taacacaaga
     7381 agaatttact aaaaatcccc ctgttattcc agcaggacac ccttattata atcttttagc
     7441 tagaataagc agagaggaaa tagaaaagga agatctatag tacaaagaaa tagtaaaata
     7501 taatagtttt gcgcgtgatg ttcgcttgaa aaggacttgt taaggtcaac aaaatcgata
     7561 accacgcgct tttatctatc tcaccttttc gatagataaa acaaccaata cgcgtgatga
     7621 acatggtata atatattcac agctggaggc ttgcacagtc tagccgatta gggagagtta
     7681 gaccgagatg tcacacactc ccttttttgg tataataaac ctatcagata agcaaaaaca
     7741 actaaaaaag cgtagctgat gactacgcta gtttcttgcc tgctgaactc gtcaaatctt
     7801 agcccttttg tggggctttt ttgctcccct ttttgcgtac tttgggggag caagtagtaa
     7861 aaagtaatat tagtgttttt tgttaaaaag cgcgtgatat aagggttagg aagccctaag
     7921 gaaacataaa ataagaagtt ttttattata ctacaaaatc ggccttttgg gtaatagaat
     7981 ctcacttttt ttggtataat ggtaagcaat aatggactag aaagaacaaa gatgcaagat
     8041 aaaattgtca ttcatggggc gcgtgcccat aatttaaaaa atattgatgt ggagattccg
     8101 cgagacaagt tggttgtcgt gactggcttg tcaggttcag ggaagtccag tctggctttt
     8161 gatactctct atgcggaggg acaacgtcgc tatgtagaga gtttgtccgc ctacgctcgt
     8221 cagttcttgg gaaatatgga aaaaccagat gtagatgcca ttgatggtct cagcccagct
     8281 atttccatcg accagaaaac gactagtaaa aatcctcgct cgacggtggg aacgacgact
     8341 gaaatcaatg actatctgcg tctcctctac gcacgtgtgg ggacgcctta ctgtatcaac
     8401 ggacatggag ctatcaatgc ttcttctgtg gagcaaatcg ttgataaggt tttggagtta
     8461 cctgaacgcc agcgcttgca gatcttggct cctgtcatcc gcaagaaaaa aggccaacat
     8521 aagagtgtta tcgagaaggt tcagaaagac gggtatgttc gtgttcgtgt ggatggggaa
     8581 gtctatgatg tgaccgaagt gccagagttg tctaagagca agcaacacaa tatcgatgtc
     8641 gtggttgacc gtattgttat caaggagggc attcgtagtc gtctctttga ttccattgag
     8701 gctgcccttc gtattgcaga aggttatgtc attatcgaca ccatggacga ttcggagttg
     8761 ctcttctctg agcattatgc ctgtccagtt tgtggtttta ctgtcccaga gttagagcct
     8821 cgtctcttct ctttcaatgc tccttttggc tcttgtagtg agtgtgacgg cttgggcatc
     8881 aagctggagg tggatactga tttggtagtg ccagatgcca gcaaaacctt acatgaggga
     8941 gcgctggcac cttggaatcc tatctcatcc aactactatc caaacatgtt agagcaggcc
     9001 atgaaagtct ttggagtggc tatggataag ccttttgagg acctgtcaga agaagataag
     9061 aacttgattc tctacggctc agatggtaag gaattccatt tccattatga aaatgaattt
     9121 ggtggtgtgc gcgatatcga cattccgttt gagggagtta tcaataatat caaacgtcgt
     9181 taccatgaaa ccaatagcga ttacactcgc actcagatgc gtctctacat gaatgagctg
     9241 acctgcggaa cctgtcaagg ctatcgtctc aatgaccagg ccttgtctgt ccgtgtgggc
     9301 ggccagcaag ggccacatat tggagaaatc tcagacctgt ctatcgctga ccacttggac
     9361 ttggtgagcc agttaacttt gtctgaaaat gaagccatca ttgctcgtcc cattctcaag
     9421 gaaatcaagg atcgtttgac cttccttaat aatgtgggtc ttaactatct aaccctgtca
     9481 cgttcagcag gaaccctttc aggtggggaa agtcagcgca ttcgtttggc aacccagatt
     9541 ggttccaacc tatcaggtgt cctctatatc ctagacgagc cgtcaatcgg tcttcaccag
     9601 agggacaatg accgcctgat tgccagtctg aaaaagatgc gtgacttggg caatactctt
     9661 atcgtggtgg aacacgacga agataccatg cgtgaagctg attatctgat tgacgttggt
     9721 cctggtgccg gtgtttttgg tggtgagatt gttgcagcag gtacacctaa gcaagtggct
     9781 cgcaacagca agtctatcac aggtcagtac ttgtcaggca aacgtgtcat cccagtacca
     9841 gaagagcgcc gtgtcggaaa tggtcgtttt attgaagtga caggagcgcg tgagaacaac
     9901 ttgcaaaatg tcacggctcg ctttccacta ggaaaattta tcgcagtgac aggtgtgtcg
     9961 ggttcaggga aatcgactct aatcaacagc attctcaaaa aagccattgc ccagaagctc
    10021 aaccgtaatt cagacaaacc tggtaagttt aagactatta cagggattga gcatgtcgac
    10081 cgtttgattg atattgacca gagccctatc ggacggacgc cgaggtctaa cccagctacc
    10141 tatacaggag tttttgacga tatacgtgac ctttttgctc agacaaatga agccaagatt
    10201 cgaggctaca agaaggggcg cttcagtttc aacgtcaagg gaggtcgctg tgaagcttgc
    10261 tcaggtgacg ggattatcaa gattgagatg cacttcttgc cagatgttta tgtggcttgt
    10321 gaagtttgcc acggaacccg ctataacagt gaaaccctag aagttcacta caaggaaaag
    10381 aatatttcgc aggtcttgga tatgacggtc aacgatgcgg tagaattttt ccaacacatt
    10441 ccgaaaattc aacgcaaact ccagaccatc aaggatgtag gtctaggcta tgtgaccttg
    10501 ggacagccag ctaccaccct ttctggggga gaagcccaac gtatgaagtt agctagcgaa
    10561 ctccacaaac gctcgacagg aaaatctttc tacattctgg atgagccgac gacagggctt
    10621 catacagagg acattgctcg cctgctcaag gttttagctc gctttgtaga cgacggcaat
    10681 acagtcctcg tcatcgagca caatctggat gttatcaaga cggcagacca tatcattgac
    10741 ttgggacctg agggcggtgt cggtggtgga accatcatcg taacaggaac tccagaagaa
    10801 gtagctgcca acgaagccag ctatacagga cactatttga aaggaaagtt acatcatgaa
    10861 taaacgcgta caagcatttc tagctaaaat acaagaaaaa gaactagatg gtatcatcat
    10921 caacaatctt aaaaacgtct attatttgac tggtttttgg ggctcaaacg gaacagtctt
    10981 tatcagccgt gaccgtcagg tcttagtgac agactctcgc tatatcatcg cagctaagca
    11041 agaaaccagt ggttttgaga ttgtggctga tcgtgatgaa ttggctgtca ttgcaggaat
    11101 tgttaaggac atgggcttga ctcgtatcgg ttttgaagat gagatttcag tgtcttatta
    11161 ccaccgtatg caggcagctt ttgcaggttt gaacttgctt ccacaaactc agtttgtgga
    11221 aggtcttcgt atgattaagg atgaggcaga gattgcagcg attcgcaagg cttgttctat
    11281 ctcagaccaa gctttccgcg atgcgcttga ctttatcaaa ccaggaaaaa ctgaaattga
    11341 gattgccaac ttccttgatt tccgcatgcg tgagttggga gcatctggct tatcttttga
    11401 tacgatccta gctagcggta tcaattcttc taaaccccat gcccatccaa tgcacaaacc
    11461 agtggagttg ggagaagcca ttaccatgga cttcggctgt ctctatgacc actatgtcag
    11521 tgatatgacc cggactatct atctagggca tgttagcgat gagcaggcag agatttacaa
    11581 tacggttcta aaagctaacc aagccttgat tgaccaagct aaggcaggct taggtttccg
    11641 tgactttgac aaaatccctc gtgatattat cattgaggca ggttatggtg actactttac
    11701 tcacggcatt ggccacggta ttggtctgga tatccatgag gaaccctact ttagtcagac
    11761 ttctacagaa actattaaga caggtatggc cttgaccgat gaaccaggta tctatatcga
    11821 aggcaaatat ggcgttcgta tcgaggatga tatcctgatt acagagacag gttgtgaatt
    11881 attgacccta gctccaaaag agttgatagt catttagttg tttgtcaaga aaaaagtacg
    11941 aaaatgacga aaaaagtcaa aaaaaattaa aa
//
LOCUS       DAVKQL010000058        11968 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVKQL010000058 DAVKQL010000000
VERSION     DAVKQL010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11968
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.058"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            <1..116
                     /locus_tag="VW561_001698"
     CDS             <1..116
                     /locus_tag="VW561_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEU9943929.1"
                     /translation="TLEDVMNQLQDVIQGLEKEVIKSIVNRRWTRMLFESR"
     gene            complement(237..2012)
                     /gene="spxB"
                     /locus_tag="VW561_001699"
     CDS             complement(237..2012)
                     /gene="spxB"
                     /locus_tag="VW561_001699"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEU9943930.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAISKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            complement(2219..4462)
                     /locus_tag="VW561_001700"
     CDS             complement(2219..4462)
                     /locus_tag="VW561_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU9943931.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTTIMLVAGKSYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDVIGDEVEVFRLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQTLHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            complement(4472..4843)
                     /locus_tag="VW561_001701"
     CDS             complement(4472..4843)
                     /locus_tag="VW561_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU9943932.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHTNLPMGEEYVVEITPE
                     QAGEFGFACGMNMMHGKMIVE"
     gene            complement(4854..5249)
                     /locus_tag="VW561_001702"
     CDS             complement(4854..5249)
                     /locus_tag="VW561_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEU9943933.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     SRLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     regulatory      5433..5531
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5615..6406
                     /gene="thiD"
                     /locus_tag="VW561_001703"
     CDS             5615..6406
                     /gene="thiD"
                     /locus_tag="VW561_001703"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU9943934.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQVRDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESIFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(6616..7248)
                     /locus_tag="VW561_001704"
     CDS             complement(6616..7248)
                     /locus_tag="VW561_001704"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU9943935.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPV
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLKKLHNILS"
     gene            complement(7241..8044)
                     /locus_tag="VW561_001705"
     CDS             complement(7241..8044)
                     /locus_tag="VW561_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU9943936.1"
                     /translation="MQALTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRMAALYANQSSLPMVVDAVGVTTSSIRKS
                     LVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLIVSENQVAVLENGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            complement(8044..8568)
                     /gene="thiW"
                     /locus_tag="VW561_001706"
     CDS             complement(8044..8568)
                     /gene="thiW"
                     /locus_tag="VW561_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEU9943937.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFAERID"
     gene            complement(8573..9265)
                     /gene="tenA"
                     /locus_tag="VW561_001707"
     CDS             complement(8573..9265)
                     /gene="tenA"
                     /locus_tag="VW561_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEU9943938.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKVFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEINDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(9276..9926)
                     /locus_tag="VW561_001708"
     CDS             complement(9276..9926)
                     /locus_tag="VW561_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEU9943939.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRIFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            complement(9928..11313)
                     /locus_tag="VW561_001709"
     CDS             complement(9928..11313)
                     /locus_tag="VW561_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9943940.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQVI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILFQNPNHQFTMENLFEELMFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAAMAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMDRTTCEFGNRSLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGTVLKKKKSLYRELTSIVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            complement(11314..11874)
                     /locus_tag="VW561_001710"
     CDS             complement(11314..11874)
                     /locus_tag="VW561_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9943941.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNIFSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
BASE COUNT         3585 a         2658 c         2318 g         3407 t
ORIGIN      
        1 gaactttgga agatgtcatg aatcaacttc aagatgtcat acaaggactg gagaaggagg
       61 tgataaagtc catcgttaat cggagatgga ctagaatgct ttttgaaagc agatgagtat
      121 tatatgcaat ttctttatat aaaaagaccg gattgctccg atcttttaaa gttctgctct
      181 atgaaaatca aagaataaag tctacaagtt tcatatttga ttttcggcga gaggaattat
      241 ttaattgcgc gtgattgcaa tccttcttct tccaagaaga gacggaatgg tacgagttct
      301 tctgcttcgt atttttcctt gaaggctttg atagcttctt ctgagtgaag ttttggatcc
      361 aattcaagta cttctactgg aagtggacgg tgttgagtga tgcgagcatc gatgacaaca
      421 gttttacctt ctttgttcaa tttaacagct tctgcaacaa ctgcatcgat gtcttcgata
      481 cggtcaactg tgaatccaac agctccttga gcttccgcaa ttttagcgta gtcagcgttt
      541 gtgaagtcta caccaaacaa gtgtttgttt gtatcttcgt atttgttctt gatgaagccg
      601 tactcagcat ttgagaagac aaggttgata actggaaggt cgtattgaac gtttgtgata
      661 acgtctgggt agcacatgtt gaatgctccg tcacccatga tgttccatac ttggcgatct
      721 ggattgtctt tcttagcagc gataccacca ggaagggcaa tacccattgt cgcaaagagt
      781 ggagatgtac gccacatgtt cttaggtgtc atgtgaagat gacgagtaga tgtttgagta
      841 gtgttaccta cgtcgattga gtagatagcg tcttgatcag catgtttgtt gattgcattg
      901 taaacttgat acaattgcaa ttcaccctca gttttacctt cgagtttgtt catgtaatca
      961 cgccagtttt ggttgttctt aacgtttgca cgccaccatg gagttgattc aactgggttt
     1021 actttgtcaa ggatagcttt agctgcttga ccagcatcac caaggattga agcgtcaagg
     1081 gcatgacgtt taccaagttt gtaagggtcg atatcaactt ggatgaattt ttcagtgttc
     1141 ttgaatgctt cgtaaacttc agcaaatggg aagtttgaac caaggaaaag aactgtgtct
     1201 gcttcaaaga ccacttcgtt ggctggtttc caaccaacac gataagcaga acctgtcaaa
     1261 ccttcatagt tccattcgaa agcttcaaag tttttaccag ttgtgatgat tggtgctttg
     1321 attttacgtg acaattcagt aatcacttca ccagctttaa caccaccaaa tccagcatag
     1381 ataactgggc gttcagcatt gttcaagatt tcaacagctt tgtcgatttc aacttcgttc
     1441 aaagcaggag cgatgaatga gcgttcgtat gaacctgaac cgtagtatga gttttcgtcg
     1501 atttcttgga aaccgaagtt tactggaatt tcaacaacag ctggaccttt tttagaaatt
     1561 gcagcacggc aggcttcgtc aattactttt ggcaattgct cagcgtaagc tacacgtttg
     1621 ttgtaaacag cgataccgtt gtacattggg ttttggttaa gctcttggaa agcatccatg
     1681 ttcaattcgt taactggacg tgatccaagg atcgctagga atggagtgtt atccatagct
     1741 gcatcgtaaa caccgttaat caagtgagtc gcacctggac cacctgaacc aactgcaacc
     1801 ccgattgagc cgccgaattt agcttgcata accgctgcaa gagcacctgt ctcttcgtgg
     1861 cgaacttgta agaagcggat atctttgtct tcagccaaag cgtccatcaa tgagctgagt
     1921 gttcctgatg ggataccgta gattgtatct acgccccatg ttttcaatac gttaagcatt
     1981 gctgcagatg cagtaatttt cccttgagtc ataatgataa ctctccttca atttttttag
     2041 atttggagaa tacgattaca tagaattgga aacgttctcc aaatttttac tattccactg
     2101 tatcatattt atgctgactt ttctaaaaat ctgctcaaaa ctctctattc tctattctaa
     2161 tacagttttg aaagttctgt catttctgtt ttataacaaa gaaatctagt cattactttt
     2221 agtctatttt actaaaattt aacagaaggg aactggtcag aacagataca gaactaaagg
     2281 ccatggctag acctgccaat tctgggttga gagccagtcc aacacctgaa aagactcctg
     2341 ctgcaatcgg aattccgaca acattgtaga taaaagccca gaaaagattg agtagaattc
     2401 gatgaaaggt tttcttactc atatcaaagg cacgaaccac tcctaaaaga ttattggttg
     2461 tcaacaccaa atctgctgac tcgatggcga tatctgttcc agctcccata gcaatcccca
     2521 catctgctac actaagggca ggagcgtcat tgataccgtc cccaacaaag gctactttcc
     2581 ctgactgttg cagtttatgg atttcatggg ctttttcttc tggcaagacg cctgcaatga
     2641 cctcttcaat tccgatttga tctgcaatag cacgcgccac accagcattg tctcctgtca
     2701 gcatgactgt tcggagacca cgttttttta gctgactgat ggctagctta gcattttcct
     2761 taggaatatc ttgcaaagca agcaagcctt tgatttcatt gtcaacagct aagaacacaa
     2821 ctgtcttagc ttctttttct agttcttcta gtttatcttg ataagtatta gaaatatcca
     2881 tgccatccag cattttagca tttccaagta aaacttgttt tccattgatt cgccctgaaa
     2941 cacctttccc gtgcaaggtc tgaaaatttt caacagtttg aaactcaagt ccagcttcac
     3001 tcgctcgctt aacgatagcc tcagccagtg ggtgttgaga agcatcttcc aaggaggctg
     3061 ccaacctaaa cacttctact tcgtcgccga tgacatctgt taccacaggt ttcccttccg
     3121 tcaaagtccc ggtcttatca aagacaaggg tttgaacttt ctggatttcc tgtaagacag
     3181 ttccattttt gaggagaacc cccatcttgg cactacgtcc tgtccccacc ataagggctg
     3241 tcggtgttgc aagtcccaag gcacaaggac aggcgataat caaaaccgcc actccgtaga
     3301 gaagagagga cacaaagcta gctccaagca caaccacact atccctgagc aagacgaacc
     3361 aaacccaaaa ggtcatgatt cctaaaatga caactactgg gacaaaaatc cctgaaatct
     3421 tatccgtcaa gtcttgaatc ggcgcacgac ttgtctgagc tttcttcaca aaatccacaa
     3481 tctgagccaa aacagtctct gagccaactt tttctgctct aaagacaagc gttccactat
     3541 gattgatggt tgagccaatg acagtatctc caactgtctt gtccacaggc agactctcac
     3601 ctgtcaccat ggattcgtca atactagaga caccttctac tacgacacca tcaacagcaa
     3661 tcttttcacc gggacgcact cgaatcaggt cgcctacctt gacttgttcc aaaggaactt
     3721 ggacataact atcatcactc aagacttctg cggttttagc ttgcaagtcc agtaatttct
     3781 ccacagcttg ggacgtattt tttctcattt tttcctcaaa aactgctccc aaaagaacga
     3841 aaaagaggat aaatccagca ctttcgaagt aaacagggag accagcaaag agagcaacta
     3901 ggctatagaa ataagccact agagttccca gcgcaaccaa ggtatccatg ttggcattgt
     3961 gctttttaaa actggcccaa gcactctgga tatatgactt acctgcaact aacataatag
     4021 tcgttgttgc tagaaaagtt ccccaatgca tgacttgatg actaatgcta cctgtcaaca
     4081 tcccaatcat gagaatcaca agaggcacag taaagatact agtaatccaa aaacgttgca
     4141 ggagagatag agattttcga gtcttctcaa cgactgtata gcttcccttt tgcatcttca
     4201 tgccacaaga aaattcatgt cgccctaatt cttgaggtgt aaaacgaatg actttctcct
     4261 catctacgcc gattggttcc aagatacctt cttcttcaaa cagaatttcc ttataacagt
     4321 ttgaaggagt agcacgatga aaggtaatct cagctggaat tcccttttga agctggatat
     4381 gggctggatg atagcctttt tcagctcgga tacggatttt ttgaatgcca ttttctaagc
     4441 ttgctttcac aatttctgtc atagtctcca cctactctac aatcatcttg ccgtgcatca
     4501 tgttcatacc acaagcaaag ccaaactctc cagcctgctc aggcgtgatt tccactacat
     4561 actcttcccc cattggcagg ttcgtatgta caccaaaatc tggaaaaaca atttgatcca
     4621 gacatggtga aggatccttg cggtcaaata caatgcgtgc tggcactgat ttcttgagga
     4681 caatcaactc aggagtatag cctcccatga cttccactcg aatctcttgg tatccatttt
     4741 tttgctgggc tttttgtcca gatttttcag gctttttgaa aaaccaaaac aagataaacg
     4801 cgataagggc aatacaaata atggttacaa tactatttaa catgacgtct cctttacata
     4861 caattacatc ttacttctgt tacagcactt gatttcttct ctgaaatcac agcttccaag
     4921 tcttccaagt cagtctgagt aaattcacat tctacaatca agtcagccaa caaattccta
     4981 atcctacggg aacaaacctt gtctttgata tcttggacaa gtaaatcccg actttggtct
     5041 agagttaaaa gggctgaata aacaaaggac ttgccttctt ttttccgagt caaacactct
     5101 ttctcaacca gccgagacaa aagtgtctga accgttgact tggaccagtc gaaccgctct
     5161 gccaaaaccc taatcaaatc tgtactggtc tgctccccct gcatccaaat aatcttcatg
     5221 acctgccatt ctgcatctga aatctgcatt accatacctc caaaatctac atttgtcaat
     5281 tacactcatc agtatactct taaaatctac atttgtcaat tatagaaata atattttctt
     5341 cgaaaaatag aattttaatc atttgaaaaa cgatttgcag tcaaatatta ctatataaac
     5401 aataaaaata tgctatacta aagaaaaaag aaaacaacca ctaggggtgc gtaaagctga
     5461 gattaacgac tgttagatcc ctctgactca atctaggtaa tgctagctga tggaagtgga
     5521 aatgataatg gggactagca gtcttctatt gcctttctaa aacagactag cttgttctta
     5581 agaatacaaa cttcagttgg ttgggaggtt ttagatgact tatttacccg ttgctttgac
     5641 cattgcaggg actgacccta gtggtggtgc tggcattatg gcagatttaa agtcattcca
     5701 agtgagagat gtctatggaa tggctgttgt aaccagtctt gtcgctcaaa ataccagagg
     5761 tgttcagcta atcgagcacg tttctcctca aatgttgaaa gcccaattgg agagtatctt
     5821 ttctgatatt ccacctcagg ctgtaaaaac tggaatgttg gctactactg aaatcatgga
     5881 aatcatccaa ccctatctta aaaaactgga ttgtccctat gtccttgatc ctgttatggt
     5941 tgctacaagt ggagatgcct tgattgactc aagtgctaga gactatctca aaacaaactt
     6001 actacctcta gcaactatta ttacgccaaa tcttcctgaa gcagaagaga ttgttggttt
     6061 ttcaatccat gaccccgaag acatgcagcg tgctggtcgc ctgattttaa aagaatttgg
     6121 tcctcagtct gtggttatca aaggcggaca tctcaaaggt ggtgctaaag atttcctctt
     6181 taccaagaat gaacaatttg tctgggaaag cccacgaatt caaacctgtc acacccatgg
     6241 tactggatgt acctttgctg cagtgattac tgctgaacta gccaagggca agagtcttta
     6301 ccaggcagtt gataaggcca aggcctttat cacaaaagct attcaagatg cccctcaact
     6361 cggtcatggt tctggtccag tcaaccatac aacttttaaa gattaagaaa aaaaactctc
     6421 tagttcccac tttaagggaa ttagagagtt tttatactct tcgaaaatct cttcaaacta
     6481 cgtcagcttc catctgcagc ctcaaaacac tgttttgagc tgacttcgtc agtcttatct
     6541 aaaacctcaa ggcagtactt tgagcaacct gcgactagct ttctagttta ctctttgatt
     6601 ttcattgagt attaattagg aaagaatgtt atgcaacttt tttaaaaagg cttgcgtttt
     6661 tgcctcaata tcttctgctt gcatcaaatc acgtacaaca gctacaccag ctatgccagt
     6721 gcccataagc tgatcaatat tctccgaagt caagcctcca atagcaacta ctggaatggc
     6781 aaccgtttgg caaattgttt tcaaggtcga tatcagagta atgggcgcat tttccttggt
     6841 ggtggttggg aaaatggctc ctgtacccaa gtaatctgca cctgatttct ccgcttccag
     6901 agctctttta accgttttag cggtgacacc gaggattttt tcaggaccca agacttgtcg
     6961 ggcaaccgaa actgggagtt cgtcatctcc aatatgcaga ccagcagcat caaccgcaag
     7021 acaaacatcc aaccgatcat cgattatcaa ggggacctga taggcatctg ttatttcctt
     7081 gacttgtttt gccagttgat aatattgatt ggttgtgaga tttttttctc gcaattggac
     7141 tatggtaacc cctgaacggc aggccgtctc aacttttgca agaaagcttt ccacggaatc
     7201 ttgatagcga ttggttacca gatatagtct aagcgcttct ctattcataa acctctcctt
     7261 tgatggtatc tagccaattt tcatctcttc ttaggagcga aagctgattg agtacttggt
     7321 aacgaaattc ttccaatccc attccttgaa caactatttt ctcagcagcg atattgagat
     7381 aagagactgc taagcaagaa gcttcaaaac cagtctttcc ttggctgaga aaaacagctg
     7441 ttaaggctcc aaccaagtct cctgtccctg ttatccagtc taattcagta cagccattct
     7501 caagtacagc aacttgattc tccgaaacaa taaggtcctt gggacctgtg actaagaatg
     7561 acataccagg ataggtctga caccagtctt tcaagacttg aagcaaatcc tccgtttctt
     7621 gatctttagc actcgcatcg accccaacgc cgtgatgctt taatccaaca agacttcgaa
     7681 tttctgacat gtttccttta aggaccgtag gtctatagtc taaaaggtct ttaactaagc
     7741 tcttacgaat ggatgaagtc gttacgccaa ccgcatctac taccatcggg agagaagatt
     7801 ggtttgcata caaagctgcc atgcggattg ctttttcctt ctcagctgac aaatgcccca
     7861 aattgatgaa gagagcctgg ctttgcttag taaaatcaag aacttcacgg ggatcatctg
     7921 ccatgacagg tttgcatccc agagccaaaa tcccatttgc cagcatctca caagaaatct
     7981 cattggtcat acagtgaatg agggaactag agcctatagg aaaaggattt gtcaatgcct
     8041 gcatcagtct atcctttcag caaagaaata tccttgcact tttttaaaga attcctgctt
     8101 gattaaaaat cgaaatgcaa taaaggaaat cgctgtacca atcaaggttg ctccgaaaaa
     8161 tcgaggagtg tagataaacc aactaagctt agcagccgat cctgtaaaga gcaccataac
     8221 aggataggaa acaatagaac caataatacc tgttcccaca atttctccca aggcagaaaa
     8281 gtaaaatttt cgaccgtact tataaaagag acctgctaga agggctccaa aagtcgctcc
     8341 tgtgagagct aaaggcggaa tcccttgagt cgtcatacgg ataaaggctg tcactgtagc
     8401 catagccaag gcataaacag gtcccatcat gattcccgct agaatattga ctacactgga
     8461 catcggtgcc attccctcaa tccgaaagat aggtgtaagg actacatcaa gggcaatcat
     8521 catagataaa atggtcaatt tgtgaacttg tagttggtgc tttctcatgt ttctattctt
     8581 ctcctttttc taaagactgt aaatcgctct tccatgtctg gtgttggtaa gccatctccc
     8641 aaaacttggc ttccatatga acactgatgt ggaaggcatc tagcattttt tgcttatctg
     8701 tctcatcact ttctcgatag agctgattga ccaatgctcc ctcctctctg atctgttgct
     8761 ctaactcatc cgtaatataa gtttcaatcc attgttgata gagaggattt ggtgatggtt
     8821 taagattaag tgatttgcct atatcatggt ataaccaagg acaaggaagc aagcttgcaa
     8881 aagctatggc taagttcggt tctgcaaatt gcctataaat atgagaaatg taatgataac
     8941 aggttggagc gattggatgt tgctccattt cctggtcgtt gatttccaat tccttgaaaa
     9001 attgttggcg aataaataac tcaccctcca ctaaaccctg agcattttgt ttcaagagtc
     9061 ttttcatctc ttggtttgaa gtcttatcag ccaaaagatg atagatttct gagaaaacct
     9121 tcagatagta ggcatcctga atcaggtaat agcggaaaat ggcaggttct aaattcccct
     9181 cttgtaattg taaaataaag ggatgatgaa aggaagcctg ccaagctttc ttggataatt
     9241 ccatcgcaat atctgtaaat tccataataa ctcctttata aaaatagact ggtttgaagc
     9301 aataaaaaga aaagcaggta gattaatttt gtttttttag gaatataaaa aatccgatag
     9361 ctattcttca actgtgcatg ttcgtcatat ccgtgagcag atagagctct caggtaaaga
     9421 tggcgccacc taaagactgt catcagaacc ttactgtaaa tcaagggcga ccaaaaatgt
     9481 agttcttgac cacgtaatag gcaagcttct ttgagggact tgatttcttg ctgaatgaga
     9541 ggaaaggaat tgaataccac aatcaaggca taggaccaag agcgtgatag ccccttttga
     9601 gccaagtaca agagaagctc ttttagtgaa atagaggaaa caaagacaag gccgatacaa
     9661 actgtcacaa aggccctcgt tccaagcata actgcctgtg aagcatctcc gtgtaactga
     9721 actgcccagt agttggcaaa agatggtaaa atggcaagta tgatcatcca agctaacatt
     9781 ttaaatcgac ggtaatagag cataaagaga atacaaaatg cgactaccga aagattcaga
     9841 gcaatcgaag gaatgaaaga tgtttccaag gataaaatca gcaagaagag actgataatc
     9901 ggtgtctggg ttgctacttt gaccatacta tctcacctcc ccttgggtat tgctactctg
     9961 agatgtaagt ggtttggtaa tggtcacttc tttcacatgc cgaagaccct gactagtcat
    10021 ctcaatccaa taatcaacca cagaaatcaa agggtctaaa cgatgactaa tgagcagaaa
    10081 acttcttcct tgattcctct cctccacaat ccacttgcaa aaataatggc aggctctatc
    10141 atccaaacct gcaaaaggtt catctagcaa gatcacggaa gccttactgg tcaagatggt
    10201 caagagctga agaatttttt gctgaccacc acttaattga tagggactct tatcgactgc
    10261 ctgctccaaa tcaaaatatc gtaaagcttg aaaaatccgc tgatttcttt cagaatcagg
    10321 tccatctaat tgaagctcct ctcgcagact gactcggata aactgcttct cagcttcctg
    10381 aacaatactc gtcagttcac ggtacaaact ctttttcttt ttcaggactg tccccttcca
    10441 tgcaatgcgc cccttatact tttgaaattg aagaatagac cgaaagaggg ttgatttccc
    10501 aacaccattg tcacccagga tacaggaaat tccttggtaa aatgtaaaat cagcaatcga
    10561 aaagagggag cgatttccaa actcacaagt cgttctatcc atatggaata gttccgggct
    10621 agaagcaact tcctttgaag caacctgtgt catctcatag gaagggattt gaaacacttc
    10681 ccttagtttt ccgtctctta gttcaaccat atggtcgata taggctttat agtcagataa
    10741 atcatggtcg cacaaaataa ctgtcttccc atcaagagcc aactctttta gaatctccaa
    10801 tatctcgatt ctgctcttgc ggtcaatgga agcgaagggc tcatccaaga gatataccct
    10861 aggattcatg gcaaagagga cagccatcgc tgctttttgc ttttccccac ctgataggtg
    10921 atgaatggga cggtgtaaga ttgcctcgca acgacattgc tggacaacct ctgctatttt
    10981 agaatcaatt tcctgaaggt gatagccgat attctccaag gtgaacatca gctcctcaaa
    11041 caagttctcc atggtaaatt gatgattggg attttgaaag agaatgccaa ccttctggac
    11101 acgtgcgatg atagaaagct gactgacctc gctcccatct atcaggactt gaccactata
    11161 gggaagagaa ctgacttggg caatgacttg aaaaaggctg gattttcctg aaccactgct
    11221 cccaactaac aaggtaaagg cttgcgcatg aaaagtaaaa tcaaacggct cagagaagat
    11281 tggggactga atcgctcgta gttccagacc catctatgct tttcctccag ttgcaaactg
    11341 atgatagagt ttgacaatgg cacgaaccaa gatggtacag aagaaataaa cagaaataaa
    11401 acgtaccaca agtaaggaaa ggacaaacgg aagggaaaag gcgtagtaac ctaacttaat
    11461 gtattcatag acaaagctaa caagcgtaat cccaatacta ttagcagtta gagagagcca
    11521 actttcatag cgattcttag ttacgataaa accaaattca cttcccaaac cttgaaccaa
    11581 gccagacaaa agagctccta gaccaaattg gctaccataa aggacttcag caagcgcagc
    11641 tagcacttct ccaatcgttg cacttccgac tctcggaaca aagatggcag caatgggcgc
    11701 agccatacac cagagaccga agaggatttc attggcaaag gcctgcaaac caagaggtgt
    11761 taagagtaga ctgaaaatat tatacacata tcctgaacca acgaaaaccc caccaaaaaa
    11821 gatagacaag aaagcaagca agataacatc ttttaactgc cattttttca acataaaaaa
    11881 ctcctttttt aaagaaaagt gggacactca agaagaccga cctaaatact ttgtatagca
    11941 gactgaattt agaacagtac acaagaat
//
LOCUS       DAVKQL010000059        11941 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVKQL010000059 DAVKQL010000000
VERSION     DAVKQL010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11941
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.059"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            105..2378
                     /locus_tag="VW561_001711"
     CDS             105..2378
                     /locus_tag="VW561_001711"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEU9943942.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQCQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
     gene            2395..2865
                     /locus_tag="VW561_001712"
     CDS             2395..2865
                     /locus_tag="VW561_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9943943.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(2982..3137)
                     /locus_tag="VW561_001713"
     CDS             complement(2982..3137)
                     /locus_tag="VW561_001713"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943944.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDETDEVSNHTYGKATLTRIEEI
                     FEEYKSS"
     gene            complement(3408..6758)
                     /locus_tag="VW561_001714"
                     /pseudo
     CDS             complement(3408..6758)
                     /locus_tag="VW561_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            complement(6788..7363)
                     /locus_tag="VW561_001715"
     CDS             complement(6788..7363)
                     /locus_tag="VW561_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9943945.1"
                     /translation="MKKVKLGQVATFINGYAFKPQDWSSEGKEIIRIQNLTKTSKGIN
                     YYSGTIDKKYIVEAGDILISWSGTLGVFQWCGRSAVLNQHIFKVVFDKIDIDKSYFKY
                     VVEKGLQDAVKHTHGSTMKHLTKKYFDNIIVPYTNLGEQQRIASELDLLSKLILRRQE
                     QLEELNLLVKSQFACEIAIKVWRNSLKFSII"
     gene            complement(7397..8362)
                     /locus_tag="VW561_001716"
     CDS             complement(7397..8362)
                     /locus_tag="VW561_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9943946.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKAQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            complement(8480..8893)
                     /locus_tag="VW561_001717"
     CDS             complement(8480..8893)
                     /locus_tag="VW561_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEU9943947.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            complement(8890..9126)
                     /locus_tag="VW561_001718"
     CDS             complement(8890..9126)
                     /locus_tag="VW561_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEU9943948.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLQSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            complement(9186..10307)
                     /locus_tag="VW561_001719"
     CDS             complement(9186..10307)
                     /locus_tag="VW561_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9943949.1"
                     /translation="MKKVKLGEILSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLRDHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKRLITKRK
                     FQLDELNLLVKSRFNEMFGENKIFESIDNLFDIIDGDRGKNYPKSDELFSEEYCLFLN
                     TKNVTKNGFSFDTKQFITKTKDKLLRKGKLERYDIVLTTRGTVGNVAYYDELIKYKHL
                     RINSGMVILRPKTPNLNQKFIIHVLRNNNYSRVISGSAQPQLPITKLKKILLPLPPLA
                     LQNEFADFVVQVDKSQLAIQKSLEELETLKKSLMQEYFG"
     gene            complement(10320..11813)
                     /locus_tag="VW561_001720"
     CDS             complement(10320..11813)
                     /locus_tag="VW561_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU9943950.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
BASE COUNT         3634 a         2469 c         1904 g         3934 t
ORIGIN      
        1 actttattca tttttattgg attatttcct tttgaagtta acaattccct ttctgctttt
       61 tttttgatat aataacctta actgtcattc tatatggagg ttctatgcgt tttaatcaat
      121 atagttatat caattttcca aaagaaaatg tcttatctga actaaaaaaa tgtggctttg
      181 atttacaaaa tacagccaat cacaaagact cacttgaaac ctttttacgt cgtttctttt
      241 tcacttatca agacaccaac tacccacttt ctatcctagc agctgataaa aaaacagacc
      301 tgctgacatt ttttcaatca gaagatgaac tgacagcaga tattttttat actgttgctt
      361 ttcaactttt aggcttttct tatttggttg acttcgaaga tagtgacgtt tttcgtaaag
      421 aaactggatt tcccattata tatggtgact tgattgaaaa tctctatcag ttactcaata
      481 ctcgcaccaa aaaaggaaat actcttatcg accaacttgt tagtgatggt cttattccgg
      541 aggataatga ctaccactac tttaacggca agagtttggc tactttttct aaccaggatg
      601 tcattcgtga agtcgtttac gttgagtctc gtgtcgatac tgaccaaaaa gggctatcag
      661 acctagtaaa ggttagcatt attcgccctc gttttgatgg aaaaatccct gccatcatga
      721 cagccagccc ttatcatcag ggaaccaatg acaaggctag tgacaaggct ctctacaaga
      781 tggagggcga gcttgaggtt aagcttcctc acaagattga gctagagaaa cctcaactaa
      841 atctcgtcca accccaaggt aaagctgagc ttatagcaga ggctgaggaa aagctaactc
      901 acatcaactc tagctataca ctaaacgact acttccttcc gagaggcttt gccaatctct
      961 atgtctcagg tgttggtacc aaagactcga caggtttcat gactaatgga gactaccagc
     1021 aaatcgaggc ttataaaaat gtcatcgatt ggcttaacgg ccgttgccgt gcctttactg
     1081 atcacacgcg ccagtgtcaa gtcaaggctg attggtcaaa cggaaaagtt gctacaacag
     1141 gactttccta tctaggtacc atgtccaatg gtcttgcgac tacaggagtc gatggtttag
     1201 aagttatcat tgccgaggca ggaatttcat catggtacaa ctactaccgt gaaaacggtc
     1261 tggtgactag cccaggtggt tatccaggtg aggactttga ctcccttgct gagttaacct
     1321 attctcgtaa tctcttagct ggcgactata tccgtggcaa tgaagctcac caagctgact
     1381 tagaaaaagt gaaagctcag ctagaccgaa aaactgggga ctacaatcag ttttggcatg
     1441 accgcaatta tctgctcaat gcccataaag taaaggcaga ggttgtcttt actcatggtt
     1501 ctcaggattg gaatgtcaaa ccacttcatg tttaccagat gttccatgct cttcctactc
     1561 atatacacaa gcatctcttt ttccataatg gtgctcatgt ttacatgaac aattggcaat
     1621 caattgactt ccgtgagtcc ataaatgcct tattgaccaa gaaattacta ggacaggaaa
     1681 cagactttca acttcctact gttatctggc aggacaatac agctccacag acttggttat
     1741 cacttgataa cttcggtggg caagaaaact gtgaaacctt ctcacttggt caagaagagc
     1801 aagctattca aaaccagtac ccagataagg attttgagcg ctatggtaag acataccaaa
     1861 ccttcaatac agagctctat caagggaaag ccaatcagat tactattaac cttcctgtga
     1921 ctaaagatct ccacctaaac ggtcgcgctc agctcaatct tcgtatcaaa tccagtacaa
     1981 acaaggggct cttatctgcc caactgctag aatttgggca aaagaaatac ctacaacctt
     2041 atccagctat tttaagtgct agaaccattg ataacggtcg ctatcacatg ttggaaaatc
     2101 tctgtgaatt gccatttaga ccagaggcac aacgagtcgt gacaaaaggt taccttaatt
     2161 tacaaaatag aaatgattta ctgttagtag aggatattac tgcagatgaa tggatggacg
     2221 ttcaatttga actgcagcca actatttaca agctaaaaga aggagacact ctccgtttag
     2281 tcctctatac tactgacttt gaaatcacca tccgtgacaa tacagactac cacttgactg
     2341 tcgacctcgc tcagtccatg cttaccttac cttgctaaaa ggagttacat tattatgaat
     2401 aaatcagaac accgccacca acttatacgc gctcttatca caaaaaacaa gattcataca
     2461 caggctgagt tgcaagccct tcttgctgag aacgacattc aagtaaccca ggcaaccctc
     2521 tcacgcgaca tcaaaaatat gaacctatca aaagtccgcg aagaagatag cgcttattat
     2581 gttcttaaca atggttccat ctcaaaatgg gaaaaacgtc tcgaactcta catggaagac
     2641 gcccttgtct ggatgcgccc agttcaacac caagtcctac taaaaaccct tcctggactg
     2701 gctcaatcct ttggttctat cattgatact ttgagcttcc ctgacgctat cgctaccctt
     2761 tgtggtaatg atgtctgtct tatcatctgt gaagatgcag atactgctca aaagtgcttt
     2821 gaagaactga aaaaattcgc cccaccattt ttctttgaag aataataaat gactacaact
     2881 aacaattatg cttaaaaaaa aactggtaga aagtctttaa catcagaaaa cgcatagtat
     2941 caagtgttga aaacccttga tactatgcgt tttgctttat cctaggaaga tttatactct
     3001 tcgaaaatct cttcaatccg cgtcaacgtc gccttgccgt aggtatggtt actgacttcg
     3061 tcagtttcat ctacaacctc aaaacagtgt tttgagcagc ctgcagctag tttcctagtt
     3121 tgctctttga ttttcattga gtattatttc attttctcct gcaattgaat tcttgctcag
     3181 ctttttgttt tctatttctt taaaatcaaa gtagctcttt tgttaataac tcgatcaaca
     3241 aacatcgtgg tacaagtatc tactttgaaa tttatcaacc acttaacaac tgatactgta
     3301 ttctaggaaa acgatgacat tcttcctaat aaaacttctc atatatagca taaatttcta
     3361 ctctttttaa ttttaaatga gtctcagcat ggcaatcact ttagaattta agccgcttct
     3421 ccagctcgat tattgataag ttcaatgatt tgaacaatac tacgaagtac tggtagttgg
     3481 tgttggaaga gtagttgaac agatccataa gatttaaacg gctcttgcgt taacactttc
     3541 gtctctaaaa aaccattttc tacaatgtag tctacaatca attttacaaa tgaaatctgc
     3601 ctggcattaa gattctcatc cgacaaaaat ttagaaaaaa tacgattggc agactctcta
     3661 tctaagccaa taatctcacg aaccaatctc ggaattgcct tattttcata atgactttgg
     3721 tagtctgctt tactacctaa tttttcccaa agcaattttt caagtgcaag catatcatca
     3781 gatgtcaact ttttattatg gtatagctta gaaatggact cctcatccag atgagttttc
     3841 aaataatgct caactttttc attgtaagac cgaagatcgt tgacctgcaa aaatgctgtg
     3901 gtctcgtgaa cagtggagag tatacgatct tcaaagttaa catagtaggg tttacggtct
     3961 gttttatcca aaaactgtaa taaatctcga atagcaagac gaattttttc caaatcagac
     4021 aagttaactt ctttccaaaa ttcaggctcc tgtactttcc tgataatttc agcctgctca
     4081 aaaacctgcg ggatattgcc aatagcagaa agagctctag ccgtcttcat cacttgggaa
     4141 atatgaacag tggaagattt agctgtcagt tgccccaact gaatatgaag caaccacaaa
     4201 tcaaatctcc tcgccatctc atcttcttta tcttcatcaa ataaaagcgg agagagattt
     4261 ttttgaatgg tttcacttgt aacagcagtt agattctgcc aactttccaa tttcctatag
     4321 ctataaactg tatctaaaac catacgaacc ctgaagtcca actcatttaa gctctctata
     4381 cgaccttgaa gttccgagac aagctgctga cggtatgctc tcgcaaactg atcttcttgg
     4441 tattggagtc cctgaagttc tcgaatcaag tccactttga tattaaataa acgctgagtc
     4501 agcgaaacaa tgtgacgacc ctctccatct cttggatctg cacgaaaata atcaaaattg
     4561 tccccataat caaataccaa gaagttttcc ttatcctgct caggtccaaa taaatcttta
     4621 catagacggg ttcctcgacc aatcatctgc caaaacttag ttttagagcg tactttcttg
     4681 aagaagacta aattaacaac ctctggtaca tcaatacctg tatctaacat atcgacagaa
     4741 atcgcaattt gaggatactt ctccttaatt ttaaaatcat caatcaaggt ctgataatgc
     4801 ttaatactat aatcaatcac ctgagcatag tcgctccctt tttcaggata gcggttgtta
     4861 aaaatacctc tgatatattc cgcatgatca tgatttttag caaaaataat agttttacca
     4921 atttcatcac ccgaggctgt ctgaattcct cttgtcatga gttcatttaa aacaatttct
     4981 actgtacttt tattgaaaat aaaggaatta aatacactcc catcaatatc tttttcacag
     5041 ctatcgtctt caaatttgct atcaaaatgt tccttttctt cttcggacaa atcatcataa
     5101 tgtagaccat ccgtaggtag tttcagtttg gtttcgatag aatgataggc tactaaatat
     5161 ccgtctttaa cagcctcttc caaatcatat gcatatgttg gaaccccatt ctccaaatta
     5221 aagaatccat aggtgttttt atctaaatct tgacgcggag tagctgttaa gcctacaatt
     5281 cttgcatcaa aataatcaaa aatggacttg tatttctgat aaatagaacg gtgagattcg
     5341 tcaattatga taaggtcaaa atgcccaaca gtgaaagggc gttgatttac ttcttcttga
     5401 ccactaatcg ctccaatcat ggtcggataa gttgaaaaga ccatgcgact tgattgagct
     5461 ccttctttat cttctaagaa gttacaaacg gaaagatctg ggagtaattt tctaaacgaa
     5521 tcataggctt gcttaaccaa ggaagttcta tcggctaaga agagaacgtt ttttacccag
     5581 ttatgacgtg ataagatatc aactagagaa actgctgtac gagttttccc agccccagtt
     5641 gccataacca aaagtgcctg tctacgatga ttagagaaag cttcacaaac gcttgcaatg
     5701 gcgtgttttt ggtagtgacg cccggaaata tcgtccctaa ttttactaga aatatcctca
     5761 agtggttttt gaagatgacg tctatccatc actaattgca attcttcttg agagtaaaag
     5821 cctgcaatct ggcggcggtt cggaccatca agtatatagt gcttcaagcc atttgtaata
     5881 aagcaaattg gctaatagcc gatatgtttc tcaagagctt cagcatattc tttgacctgt
     5941 acttccccta cttctggatt gacagaggct tttttagcct ctacaatcgc taaaattttc
     6001 ccatttttac cataaagtac atagtcacag taaccaattc ctgaaccgtg cttgagacca
     6061 tcaacggcta tctcaacacg acagttttct tcaaatatcc atcctgctaa acggagatcg
     6121 atatcaataa agagctttct tgtctctgcc tcagataatt gatcaatatg gaaaggcaca
     6181 tcttgccgtt gcgctgcagt ctcacgtttt tcagtataag tctcttgaac ttctacggac
     6241 tgagcagcca tctgttcatg aaaatcaggc agttctggta aactgtcttg taactttatc
     6301 atagattgtg gagtttctcg gtatttgatg tttgctgaaa gtggtaagca cttctcatca
     6361 aaataacgct ccacaaactc attgctgtaa caataatcga taaaattaac aaactgatac
     6421 aaatgatgca aagctaaaat cgcttctcgt tccttaattt caccaccatg agcagcatgg
     6481 tttccccacc gaatcaacag aactatctgc ttgtggagtt cagaatctac gatatcccta
     6541 aaatcatcat cccatactaa agaagataga gtagcacgat agggagcttc taaatatgaa
     6601 tcgtgactat atatccaatg gacagcctgc tctaaagcac gacgcgccat aaaggcagta
     6661 gccacagttg atgtagcaat catattctca gcttcaatgc aaggttttga aaaagattca
     6721 tattcgtcat tttttaaaag aaagctaaaa tttcccattt ttttctcact ttaaacaaat
     6781 agtttagcta tattatacta aatttcaagc tatttctcca cacttttata gctatctcac
     6841 aagcaaattg tgattttaca agtagattaa gctcttcaag ttgttcttgt ctccttaaaa
     6901 tcagcttgct taacaaatcc agctcacttg caattctttg ttgctctccc aaatttgtat
     6961 atggaactat aatattatca aaatattttt tagttaaatg tttcattgtg gaaccatgag
     7021 tatgttttac agcatcttgt aaaccttttt caacaacata tttaaaatac gatttatcta
     7081 tatctatctt atcaaaaacc actttaaaaa tgtgctgatt taacactgcg gatcttccac
     7141 accactgaaa aacccctaat gtacccgacc acgaaataag aatatcacca gcttcaacaa
     7201 tgtatttttt atcaatcgta cccgaataat aatttatccc ttttgaagtt ttagtcagat
     7261 tctgtattct gataatctct ttcccttcag atgaccaatc ttgaggttta aaagcatagc
     7321 cattgataaa agtagccact tgccctaatt tcactttctt catgcaacca cctcctcgga
     7381 atatcgggat ttgcttttaa gagatgaatt tttggtgtga atgcttgaca ttattttgat
     7441 tgaccatggc ataggccagg gttgtgtcaa tcttgctatg acctagcagt ttttggactt
     7501 gttcgatagg cataccttta tcaattgcct tagtcgctaa agttcttctg aacttatgtg
     7561 ggtgaacctt ttgtatgccg agtttgtctc ctaactttct taagcgaatc tctacaccag
     7621 caattccaag tctctgggct tttccaacta gcgttacaaa aagagcaggg tgactatctt
     7681 ttctgtcgtt aagataattt cttaaatgaa ttttcgtccg agcatcaaaa tatactggtc
     7741 tctccttctt tcctttacca aagacaacac actctctgtt ttcaaaatca atatctgaac
     7801 gattcaactg tacaagctcc cctacacgca tacctgtcga tgctagtagg tctattattg
     7861 ccaaatctct caaattttca cagttatcac gcataatttc caaatgttca tcagtataag
     7921 tttccttcac attttgctca gtcttaattt tctttatccg tcgaatggga tttttaatga
     7981 tatattcctc ttgctcaagc caagcaaaaa aagaagacaa tatacgccta atattgtcta
     8041 aatttgcctt actacaatcc ttttcagact ggtaatttgc taaataactc cttaaatcat
     8101 ctgttgtgag ttgtgtcaca gactctttaa tagcattaaa caagttttca atcgtcctct
     8161 ggtaataacg aattgttttt gagctacagc cctctacctg cttggcggta ataaatagtt
     8221 gcaacaggtt attattcgaa tacttctgct gcttttttcc gtctactaga tgatgttcta
     8281 aactctctct caacgctagt aattgttcat tatttaaaag aggtaacatt tcttgcatga
     8341 taattgtaac ttttgattcc ataatttctc actttcttat tcatcaataa aaagagacaa
     8401 tatcagtttc tgcattacag aattctaata ttgtctgttc atttattttc ttgtgctcat
     8461 tcaaatccag catactaggt tactttttga ccttttctct aacagaatgt tcagaaaccc
     8521 atttggaata gcttgtcatt ttttcatcag ttaaagcttc tactgcgatg gttacacaca
     8581 tttttactgc ttcttctacc gttacagaaa aacgatagcc gtttaattgt aaaaatttga
     8641 ccaaaacgaa gaaagcagtt cttttattag cattagcaaa aacatgcttc tttatcaatt
     8701 ggacaaatag tatcgttgct ttatcaaaaa ttgttggata aagagacttc ccaaagacaa
     8761 attgttctgg taagttcaca atcatattta aggcagaaga actaactgtt tgaattttct
     8821 catttggaga ataccgttga attgctaaag catttatttt ttcaatttgc ttttctgtca
     8881 gatagattgt catttttcca ccaaagcttt gaaaacatcg tcgtattcgt caaatatttt
     8941 atctaaattt aagtcaaagg catcatcaga tacataagaa acctgctgcc ccaattcttc
     9001 aggagtaaag ataatctcac cactttgtaa caatttcgct tcgtatttaa caccacttgg
     9061 aatattaaat tcacttggaa tcgtaatggt gattgaattt ccttgttttc ttgtttttac
     9121 taccatctga tttcctcctt tgaagtatta taacaaatca ttaccgtaat tacaatggca
     9181 gaatatcagc caaaatactc ctgcatcaga gatttcttca aagtttcaag ttcttccaga
     9241 gatttttgga ttgccaattg tgatttgtcg acctgaacta caaagtctgc gaactcattt
     9301 tggagggcta gtggggggag ggggagaagt atttttttta attttgtaat tggtaactga
     9361 ggctgagcac ttcctgatat cactcgacta taattattat ttcttaaaac atggataata
     9421 aatttctgat ttagatttgg tgtcttggga cgtaatatta ccatacctga atttatacgt
     9481 aaatgtttat attttattaa ttcatcgtag tacgctacat ttccaacagt acctcttgtt
     9541 gtcaagacta tatcataacg ctcaagtttg ccttttcgaa gtaatttatc ctttgtttta
     9601 gtgataaatt gctttgtatc gaatgaaaat ccgtttttag taacattctt tgtatttaaa
     9661 aataaacagt actcctcact aaacaactca tctgatttag gataattttt gcccctatca
     9721 ccatctataa tatcaaataa gttatcaatg ctttcaaata ttttattttc cccaaacatc
     9781 tcgttaaatc gggatttgac gagcaagttt agttcatcta actgaaattt tcttttagta
     9841 ataagccttt taatcgtatt aagaatacag ataatgttct cttgttcttc gatacctagc
     9901 aattctaatt gtaaatcaag taatatattc ttgtttaaat gaggaatagt tgcacctgtt
     9961 gaatgatctc gtaaatactg cgatttactt tccaaaaaga ctcccaagta atctgatata
    10021 attttttctt tgtatcgctc attctttttt aagaccgtaa ttgtactacc aacagctccc
    10081 gataacccat agccaactgt tcctgcatta gcaccatccc atgctattag aatatcatct
    10141 gggagtgctt cagtcatatt taaactttca gtgaatttta aattattatt atttcttaaa
    10201 tcatctattt gaatataacg ttggcttaga gttgtttgtt cagcaagtac agtggctttc
    10261 tttccttttt ttagagataa gatttccccc aacttcactt ttttcataca gccacctccc
    10321 tacttgagta atttttccaa ttcagccaag ccagcttgaa tttctttttc taaatcattg
    10381 attttcttta agatgacttc tgttggttca tactcaactt tttcatactc aatctcttta
    10441 tatttattga tagacaaatc ataatcattt tcctttatct cagcaactgg aacaaagaaa
    10501 gattgatccg ttctctgacg ttctgcttct ttttcaagat gatgaaagcg ttcgataata
    10561 tctggaatat cattgtcgct aatcggttgt cgcttatcat ccaaacttaa accatccgct
    10621 ttcatatcgt aaaaccagac tttgtcagta ccaccattac cagtttttgt aaagatgaga
    10681 atggcagttg aaactccagc ataaggcttg aacacaccac taggcattga gattacagca
    10741 tcaagcttat gattctctac aatttcctga cgaattcctt tatgagcttt agacgaacca
    10801 aaaaggacac catcaggtac gataactgct gctcgtccac ctggttttaa agttcgcaag
    10861 aaaagagaaa gaaagagtaa ttctgttttt ttggttttta cggttgcaag aaggtcatta
    10921 gaggttgaat tgtagtcaag tgagccctta aaaggaggat ttgctaaaac caaagtatat
    10981 ttatcggctt cttcattatc ttgagacagc gagtcaaggt aactgatttg tggattttct
    11041 actccatgta gcatcatgtt catcgcccca agtctcaaca tagtcgtatc cgtatcattt
    11101 ccatgaaaca tctgattatg aaaatgattg atattatctg tattggtttc ccattcatct
    11161 ttcttacgct ttaagtaacg gctagcagat actaagaagc cagcagaacc catagcggga
    11221 tctgagatga tatctttgat agtcggttgc atcaactcaa ccatcatatc gatgatgtga
    11281 cgaggtgtac ggaactgtcc atttttacct gcggtcgaca attttgataa cagatattca
    11341 tagatatctc cgatatcagt aatactttgt ttgtcattat caaaatctac atctaatccc
    11401 ctagttggaa aaacatctaa gatagaaatt gccttttgaa gcgtagcagg tttatttatt
    11461 tgaaaaatag cttctcgcat atatcgtgaa aaggctgtat catctgtatc ccccttgaga
    11521 tttttaataa acggaaaaat ctcctgagtc attaaacgat aaacttcctg agcatctcct
    11581 atatttttaa aagttgacca acggtattca ggtttatctt ttggaaaaac tccctcataa
    11641 ggaatcccta gaaattcagc atcactttca cgtccaagct cgacactatc caaatctttc
    11701 ataaataaga gataagtcaa ctgttcaata tttgttaaag gatttgcgtt tccttctgtc
    11761 caaagaattt cccacagctg atcgatttta ttttttaatt cgcctgtaat catatcttcc
    11821 cttttcttta gtttttaaaa cttagtaata tcattatttt atcagatttc aaacaaagct
    11881 tcaattaact atatctatgc gtcacctgaa tattttatac tcttcgaaaa tctcttcaaa
    11941 c
//
LOCUS       DAVKQL010000060        11562 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVKQL010000060 DAVKQL010000000
VERSION     DAVKQL010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11562
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.060"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            436..1146
                     /gene="deoD"
                     /locus_tag="VW561_001721"
     CDS             436..1146
                     /gene="deoD"
                     /locus_tag="VW561_001721"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU9943951.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLGDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKELGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(1169..1693)
                     /locus_tag="VW561_001722"
     CDS             complement(1169..1693)
                     /locus_tag="VW561_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEU9943952.1"
                     /translation="MKITIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFLPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(1746..1982)
                     /locus_tag="VW561_001723"
     CDS             complement(1746..1982)
                     /locus_tag="VW561_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEU9943953.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(2050..2970)
                     /locus_tag="VW561_001724"
     CDS             complement(2050..2970)
                     /locus_tag="VW561_001724"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEU9943954.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEEKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVEAFAHQVWTSINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            3160..3527
                     /locus_tag="VW561_001725"
                     /pseudo
     CDS             3160..3527
                     /locus_tag="VW561_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3743..4324
                     /locus_tag="VW561_001726"
     CDS             3743..4324
                     /locus_tag="VW561_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9943955.1"
                     /translation="MYYAENPDAAHDIHELRVELLGQKMTFLTDAGVFSKKIVDFGSQ
                     LLLKCLEVNQGETVLDVGCGYGPLGLSLAKAYGVQATMVDINTRALDLARRNAEKNNA
                     KATIFQSNIYEQVEGHFDHVISNPPIRAGKQVVHEIIEKSKDFLETGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            4321..5598
                     /locus_tag="VW561_001727"
     CDS             4321..5598
                     /locus_tag="VW561_001727"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEU9943956.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLVEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGKGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            5616..6278
                     /gene="deoC"
                     /locus_tag="VW561_001728"
     CDS             5616..6278
                     /gene="deoC"
                     /locus_tag="VW561_001728"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEU9943957.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKIVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            6265..6654
                     /locus_tag="VW561_001729"
     CDS             6265..6654
                     /locus_tag="VW561_001729"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU9943958.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGNVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMVEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            6745..7797
                     /locus_tag="VW561_001730"
     CDS             6745..7797
                     /locus_tag="VW561_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEU9943959.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQDWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            7941..9476
                     /locus_tag="VW561_001731"
     CDS             7941..9476
                     /locus_tag="VW561_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9943960.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADILVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGISGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            9469..10527
                     /locus_tag="VW561_001732"
     CDS             9469..10527
                     /locus_tag="VW561_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9943961.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIMIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            10530..11486
                     /locus_tag="VW561_001733"
     CDS             10530..11486
                     /locus_tag="VW561_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9943962.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPAIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         3301 a         2060 c         2644 g         3557 t
ORIGIN      
        1 ggtttgaaga gattttcgaa gagtataaac agaaaggtag agcgcgtgtt ctaatttgaa
       61 cacgagtaga aaacttttct aaaaacaaaa acgaaaggat gggtaaactg tattcgctga
      121 actgaatacg ggcgactctc ctctaaatca aaattaagaa agaaaggaat tgaacccacc
      181 ctaaaagcag tgggaaaaag atagttggtc tagcgagcat cgctcactgc gcccaactcc
      241 tattttccct tcgctttttg acgggtttgg tatctttctc aatataaaat ataaaataaa
      301 gaaaggtaga gcgtgtgttt tgatttgaac acgagcggaa aactcggaaa atagataatc
      361 tgactgaaaa atcaggattt ctcgtcggct tcctaatttt cagtcgtttt cttctcgctc
      421 tttgtatcat aaattatgtc tatccatatt gctgctcagc agggtgaaat tgctgataaa
      481 attcttcttc ctggggatcc tcttcgtgct aagtttattg cggagaattt ccttggtgat
      541 gctgtttgtt ttaacgaagt gcgtaacatg tttggttaca ctggtactta caagggtcac
      601 cgtgtatctg tcatgggaac tgggatggga atgccatcta tttcgattta tgcgcgtgag
      661 ttaatcgtag actacggtgt gaagaaattg attcgtgtgg gaactgcagg ttctttgaat
      721 gaagaggttc atgttcgtga attagttttg gcgcaggcgg ctgcaaccaa ctcaaacatc
      781 gttcgtaatg actggccaca gtacgatttt ccacaaattg ctagctttga tttgcttgat
      841 aaagcctacc atatcgccaa agaacttggt atgactactc acgttgggaa cgttttgtca
      901 tctgatgtct tttactcaaa ttactttgaa aagaatatcg agcttggtaa atggggagtc
      961 aaggctgtgg aaatggaagc agcagctctt tactatcttg ctgcccaata ccatgttgat
     1021 gcgctagcta tcatgaccat ctctgatagc ttggtcaatc cagacgaaga cacaactgca
     1081 gaagaacgtc aaaatacctt cactgatatg atgaaggttg gtttagaaac cttgattgca
     1141 gaataattat agccaaaaag gaagcccttt atgaattgta ggacttcctt ttcttatcca
     1201 gaaattgatc tagctctctc tgatttcgaa ggatagtgac tttatgtgaa tattcttggc
     1261 aaagtttttg gtaattttct ttttgagttt tgctacgccc atcccaaaga atccatctga
     1321 taaactccca ctcaaagcgt tcagggcaat ctgccgccat actttctctg acttttccac
     1381 ggtatttaag ataacgctta aaggctctaa agagacaggt caatggcaaa aaattgagaa
     1441 agatgatttg gtcagcttct tgcattcgtt cttggtagta gcaccaagaa taattaccat
     1501 cgatgaccca agctttatgc ttggtgagaa agttttttat ctcggttaac atccattcgc
     1561 agtcactgtc ttgccaacca ggttgaaatt ggagtgtgtc catgtgcagt tttggaatgg
     1621 agtagtagtt agataacttt tctgctagag ttgacttacc agaaccagaa tatccgataa
     1681 ttgtgatttt cattttctac cttttcctat ttggagacaa aaaaacagcc tctatggact
     1741 gtttcttatt tagcaagttt agctgaaaga cgagctttat cgcggcttgc tttgtttttg
     1801 tgaatcaaac ctttagtttc tgctttatcg atagctgagc tagcagcacg gaaaagttct
     1861 tcagatgggt ttgcttcgaa agcttttata gcagtacgca tagctgattt ttgagctgag
     1921 ttcttttcgt tttgtttaac gttcaattca gcgcgtttga tagctgattt aatgtttgcc
     1981 aatgttctta cctccatatt tactaactat accattatat atgaaaactt acgttttgac
     2041 aagggaaaat tattttttta agtaaatttc atcgatttca tggttcttgc ttttatgaag
     2101 aatcacttct gcacgatttc tggttggttc aatataattt tgtagatttg tgagattgat
     2161 actggtccag acctgatggg caaaggcttc cacttcccca atcggcatct gagtaaaacg
     2221 ataatagtag ctatcagggt cgttttgggc tagactcagc attttcaaga aacggtccag
     2281 ataccaactt tcaatatcat ccactccagc atctacatag atggaaaagt caaagaagtc
     2341 agtgatatag agacgatcgt tttgtggatt ttgaaagaca ttgattccct caacaattac
     2401 aaaatcagca gctttgacac tttgtttctc ttcgggtacg atgtcgtaaa cttcatgaga
     2461 atagacagga atatctacat cttgtccatt tttgatgcgg tccaagaagt tgagaagagc
     2521 ttccatatca tagctttcag gaaatccttt acgatttaaa atcccctgct caatcaaggt
     2581 ttgattggga tagagaaaac catcagttgt aaccaactca accgtagcat ctgtaaacgt
     2641 acgggacagt aggatttgaa gtaggcgact ggttgtggat tttccaacgg caacactccc
     2701 agaaacccca ataataaaag gttgagattt actttcacgt tggaggaaaa ttccttttga
     2761 aaaggctaaa tcttccttag ttcgcttgta aatctgaatc agatgagcca aggggagata
     2821 gatatctgta acgtcttgga gactgatttg gtcattaaaa ctcttgatag attccaattc
     2881 ttcttctgtc aaaggaggtg ttgtctttcg atgtaaagat tgccaagtct ggcggctgat
     2941 tttttcaaaa tgtaaaaatt cgttggtcat ttgttttccc ctaggtattt tatctatcat
     3001 accataaaaa gctaaaaaaa taaagcggtt tcttgaatgt tgtaagtcaa tgatatataa
     3061 tataagcgat caatttattg atgaaatagg agatagattc tactgattta taagcttata
     3121 aagggggctt tttagttcac aacttatagg aggtgtacta tgaaaatctt aaaacgttac
     3181 atattggaac tctgttttat tttaagtttt gctttacctt ttataaaagg agccaatgca
     3241 gataatggta gatgctttgt ggaaacctat tacggtttta cttttttgat ggaacatgct
     3301 attgtaacag ctgtctttat ctgttcgttc ttaattgctg tcttactaaa aaacgatggg
     3361 cgaaatggat tgcgacagag ggttatttct tccgcatgtc actagaagat ttgatacgac
     3421 tttggacaag tttggaaatc ctgacacaaa cgtatcagtt gggcttttat ctaaacattt
     3481 tgttgggaat tttgttgata ataaagtatt ttaaggttaa gcaatagaaa taataatctg
     3541 cctgattata gatgataaac tttttgtttt tatctggact gtgatattta tagttctaaa
     3601 tggtttatta tttataagtg ttgaaagaga ttgaagaaaa tggcttcgtg actcgctttc
     3661 ttcggtcttt ttcgttttat tatcaatttg taaacgattt acaatttgag tcaaattatg
     3721 gtaaaatagt gtcatgagta aaatgtatta tgcagaaaat cctgacgctg ctcacgacat
     3781 tcatgagttg agagtagagt tgttgggaca aaaaatgacc tttttaacgg atgcgggtgt
     3841 ttttagcaag aaaatagttg actttggaag tcaactattg ctcaagtgtc ttgaggtcaa
     3901 ccaaggagaa actgtccttg atgtaggctg tggttacgga cctttgggtt tgtccttagc
     3961 taaggcttat ggagttcagg ctactatggt tgatatcaac actcgtgcct tagatttagc
     4021 tcggagaaat gctgaaaaaa ataatgcaaa agcgacgatt ttccagtcca acatctatga
     4081 acaagttgaa ggacattttg atcatgtcat ttccaatcct cctatccgtg cgggcaagca
     4141 agtcgttcat gaaatcattg agaaaagtaa agatttcttg gaaactggtg gggatttgac
     4201 aatcgtcatc cagaaaaaac aaggggctcc aagtgctaag tccaagatgg aggatgtgtt
     4261 tggtaattgt gaaatcctta aaaaagacaa gggatactat atccttagaa gtgtgaaaga
     4321 atgagagcag ttgatttaat acaaaaaaaa cgagacggtc aagaactgac ttcaagtgaa
     4381 attgaatggc tggtagaagg ctatgtgtca ggaactgttc ctgattatca gatgtctgcc
     4441 tttgctatgg ctgtttattt taaaggaatg acgactcgtg aaatttctga tttaacgatg
     4501 aatatggtta agactgggca agagtttgac ctgtcagcca ttgatggtgt gaaagttgac
     4561 aagcattcta ctggtggtgt cggcgataag gtaaccttga ttctagctcc tcttgttgct
     4621 agcttcggtg tgcctgtagc aaaaatgagt ggtcgtggtc ttggccatac aggcggaaca
     4681 attgataaat tggagtccat taagggctat caagtagaac gtagtcaaga ggattttatt
     4741 cgtcaggttc aggatattgg agtatctgtc attgggcaat cagaccagct ggtcaaagca
     4801 gataagcttc tctatgccct tcgtgatgtg accgcaactg tcgacacgat tcctttgatt
     4861 gcgagttcgg tgatgagcaa gaaaattgcg gcaggggcgg atgctatttt gctagacgtg
     4921 actgtcggtg agggtgcctt catgaagacg gttgatgagg cgcgtgagtt ggctcaaact
     4981 atggtagatc ttggtaaggt tgttggacgg aagacagtag cagtgattac ggatatgagc
     5041 cagcctttgg gacgagcgat tggaaatcgt ctggaaatcc ttgaagcatt ggagattttg
     5101 caaggtaaag gacgtcagga tattacccac tttatttgtg aattggctca aattatgctt
     5161 ggtttggcaa atgttaacaa gactgttgaa gaagttcgcc aacatcttga gaatggtcaa
     5221 gcactggcta agtttgagga aatggtgcaa gcccaaggtg gagacttgga agacctctat
     5281 cgtcctgtaa atgtagctca tgtggtggaa atccctgctc aggaaacggg tgtcatttca
     5341 gctcttccag ctatggactt tggtctttat gccatgaggc tgggtgctgg tcgtgcagtt
     5401 aagtctgatg ccttggacta tgaaacagga attgtttttg aaaagaagat tggagactcc
     5461 gttcaaaagg gtgaaattgt tgcaaaagta tacacaaatg aaaaaattcc acctcaacta
     5521 gttacagatt tccaaaaatg tgttaaaata agtgatgaag tgaaaaaaat ccgtgaaatt
     5581 gtagaaatca tctcctaaat ggagggaaaa ccgaaatgaa attaaataaa tatatagatc
     5641 atacgctttt aaaacaagat gcaaagaaaa aacaaattga tagtttgttg tctgaggcta
     5701 gagagtatga ctttgccagt gtttgcgtta atccgacctg ggttgaacat gctaaaaaag
     5761 gacttgaagg cacagatgtt aaggtttgca cagtagtagg tttccctttg ggagcaacaa
     5821 cttcagccgt gaaagcattt gagacaaaag aagctatcca aaatggtgca gatgagattg
     5881 atatggtgat caatgttgga gctctcaaat caggtaattt agccttggtt gagtcagata
     5941 ttcgcgcagt agtggaagca agtggtgata agttagtgaa agtcattatt gaagcttgcc
     6001 ttctgacaga ccaagaaaaa attgttgttt gccaattggc ccaaaaagct ggggctgact
     6061 ttgttaaaac atctactggt ttttcaactg gtggtgctac gatagcagat gttacattaa
     6121 tgcgtgaaac agttggatct gatatgggag tcaaggctgc tggtggagct cgttcttatg
     6181 cagatgctct tgcctttgtc gaagcaggtg cgacccgtat cggaacgtca gctggggtag
     6241 ctattttaaa aggagaattg gcagatggcg actactgagt tgattgaact ggcaattgaa
     6301 accagcaaac atgcctatgt cccctattct cactttccta tcggagcagt tttagtagcc
     6361 aaagacggaa atgtttacac gggagtgaat atcgaaaatg ctagctatcc tttgaccaat
     6421 tgcggtgaga gaacagctat ttttaaggct atatctgaag gccaaagaga attttcagaa
     6481 ttgattgtct atggtcaaac tgaaaaaccg atttcaccat gtggtgcttg tcgccaagta
     6541 atggtcgaat tttttgaaca agatctaaaa gtgaccttag tcgcaaaaga taaatcgacg
     6601 gtcgagatga cggtcgggga gttacttcca tattctttta cagacttaaa ttagtctgag
     6661 tcgctctttg agtggcaagg gtctttgtga ccaatcaatc catacttgca acatcgttgc
     6721 acatcttatt taggaggttc agtaatgaac aagaaacaat ggctaggtct tggcctagtt
     6781 gcagtggcag cagttggact tgctgcatgt ggtaaccgct cttctcgtaa cgcagcttca
     6841 tcttctgatg tgaagacaaa agcagcaatc gtcactgata ctggtggtgt tgatgacaaa
     6901 tcattcaacc aatcagcttg ggaaggtttg caagactggg gtaaagaaca caatctttca
     6961 aaagataacg gtttcactta cttccaatca acaagtgaag ctgactacgc taacaacttg
     7021 caacaagcgg ctggaagtta caacctaatc tttggtgttg gttttgccct tcacaatgca
     7081 gttgaagaag cagctaaaga acacactgac ttgaactatg tcttgattga tgatgtgatt
     7141 aaagaccaaa agaatgttgc gagcgtaact ttcgctgata atgagtcagg ttaccttgca
     7201 ggtgtggctg cagcaaaaac aactaagaca aaacaagttg gttttgtagg tggtatcgaa
     7261 tctgaagtta tctctcgttt tgaagcagga ttcaaggctg gtgttgcgtc agtagaccca
     7321 tctatcaaag tccaagttga ctacgctggt tcatttggtg atgcggctaa aggtaaaaca
     7381 attgcagccg cacaatacgc agccggtgca gatattgttt accaagtagc tggtggtaca
     7441 ggtgcaggtg tctttgcaga ggcaaaatct ctcaacgaaa gccgtcctga aaatgaaaaa
     7501 gtttgggtta tcggtgttga ccgtgaccaa gaagcagaag gtaaatacac ttctaaagat
     7561 ggcaaagaat caaactttgt tcttgtatct actttgaaac aagttggtac aactgtaaaa
     7621 gatatttcta acaaggcaga aaaaggagaa ttccctggcg gtcaagtgat cgtttactca
     7681 ttgaaggata aaggggttga cttggcagta acaaaccttt cagaagaagg taaaaaagct
     7741 gtcgaagatg caaaagctaa aatccttgat ggaagcgtaa aagttcctga aaaataatgg
     7801 atggaagtca tttcttagga agcggccaag ggccgctttt ttaagagttg agacaaagtc
     7861 attttgtctc agtaaagctt gctactagac aaactagcaa gttttattga aataaaataa
     7921 gactctgaaa ggaagagcac atggcacacg aaaatgtcat tgagatgcgt gatattacca
     7981 aggtgtttgg tggatttgtt gctaacgaca aaatcaactt gcacctacga aaaggtgaaa
     8041 ttcatgcact tttaggagaa aatggggctg gtaagtccac gctaatgaac atgttagcag
     8101 ggcttcttga accaactagt ggtgaaatcg cggtcaacgg tcaagttgtc aatctcgact
     8161 ccccatctaa agcagctagc ttgggaatcg ggatggttca ccagcacttt atgttggttg
     8221 aagccttcac agtggctgaa aacatcattt taggtagtga attgactaaa aatggtgtgc
     8281 tagatatcgc tggagctagc aaagaaatca aggctctttc tgaacgttat ggcttagctg
     8341 ttgacccttc tgccaaggta gcagatatct tagttggagc ccaacaacgt gtagaaattt
     8401 taaaaacact ttatcggggg gctgatatcc ttatctttga cgaaccaacg gctgttttga
     8461 ctccatcaga aattgatgag ttgatggcta ttatgaaaaa tcttgtcaaa gaaggaaaat
     8521 caattatctt gattacccac aaattggatg aaattcgagc agtttctgac cgtgttacag
     8581 ttatccgtcg tgggaaatca attgaaaccg ttgaaattgc aggggctacc aatgctgatt
     8641 tggcggaaat gatggtagga cgttctgttt cctttaaaac agagaagcaa gcctctaaac
     8701 caaaagaagt ggttttgtct atcaaagatt tggtggtcaa tgaaaaccgt ggtgttccag
     8761 ctgttaaaaa tctgtccttg gatgttcgtg ctggagagat tgttggtatt tcggggattg
     8821 atggaaatgg tcagtctgaa ctgattcaag ccattacagg tcttcgtaag gttgaatctg
     8881 gtagcattga gctaaaagga gattcaattg taggcttgca cccacgtcag attacagaac
     8941 taagtgttgg gcacgttcca gaagaccgtc accgtgatgg cttgattttg gaaatgatga
     9001 tatctgaaaa tattgccctt caaacctact ataaagaacc acatagtaaa aatggaattt
     9061 tgaattattc aaatattact tcttatgcta aaaagctgat ggaagagttt gatgttcgcg
     9121 ctgccagtga attagttcct gcagctgcac tctcaggagg aaatcaacaa aaagcaatta
     9181 ttgctcgtga aattgatcga gatcctgatc tccttatcgt tagccagcca actcgtgggt
     9241 tggatgtcgg tgccattgag tatatccaca aacgcttgat tgaagagcgt gataatggca
     9301 aggctgtcct tgttgtcagc tttgaattgg atgagatttt aaacgtctca gaccgtattg
     9361 ccgttatcca cgatggtaag attcaaggta ttgtatcacc agaaacaacc aataaacaag
     9421 aacttggtgt cttgatggct ggtggaaact tgggaaagga gaagagtgat gtctaaaaaa
     9481 ttacaacaaa tttcggttcc cttgatttct gtatttctag gaattttact cggagccatt
     9541 gtcatgtgga tcttcggtta tgatgctatt tggggctacg aagaattgtt ctatacagcc
     9601 tttggcagtc tgcgtgggat tggagaaatc ttccgtgcta tgggtcctct ggtcttgatt
     9661 ggtcttggtt ttgcggttgc cagtcgagct ggtttcttta acgtcggact tcctggtcag
     9721 gctttggcag gttggattct cagtggttgg tttgcgctgt cgcatccaga tatgccccgt
     9781 cccttgatga ttctagcaac catcatgatt gccttgattg ctggtgggat tgtcggagcg
     9841 attccaggta ttcttagggc ctatctaggg acgtcagagg ttattgtaac catcatgatg
     9901 aactacattg tcttgtatgt agggaatgcc tttatccatg ctttccctaa agacttcatg
     9961 caaagtacag attcgaccat tcgtgttggg gctaatgcaa cctatcagac accttggttg
    10021 gctgagttga ctggtaactc acggatgaat attggtattt tctttgccat cattgccgtt
    10081 gcagttattt ggttcatgct caagaaaaca actcttggtt ttgaaatccg tgcagttggt
    10141 cttaatccac atgcttcaga atatgctggt atttctgcca agcggactat tatcctatct
    10201 atgattattt caggtgcctt ggcaggtctt ggtggagctg ttgaaggttt gggaaccttc
    10261 cagaacgtct atgttcaagg ttcgtcatta gctatcggat ttaacggaat ggcggttagt
    10321 ttgcttgcgg ccaactcacc aattggtata ctctttgcag ccttcctatt tggcgttctc
    10381 caagttgggg ctcctggtat gaatgcggcg caggtaccat ctgagcttgt cagcattgta
    10441 acagcgtcta ttatcttctt tgtcagtgtt cattacctta tcgaacgctt tgtcaaaccg
    10501 aaaaaacaag ttaaaggagg taagtaaaga tgtctattat aaccttgctc cccctcttgg
    10561 tgtcttctat gctgatttac tcagcacctc tcatctttac aagtatcggt ggtgttttct
    10621 ctgaacgtgg tggtgtggta aacgttggcc ttgaaggaat tatggttatg ggtgcctttt
    10681 ctggagttgt ctttaacctt gaatttgcag aacaatttgg agcagcaact ccatggctat
    10741 ccttgcttgt agcaggattg gttggtagtg ttttctctat catccacgca gcagcgacgg
    10801 ttcatttccg tgcagaccat gttgtcagcg gtacggtatt gaacttgatg gcgcctgcct
    10861 tggctgtttt cttagttaaa gttctttata acaaaggaca aaccgacaac ctaagtcaaa
    10921 cttttggacg ctttgatttc ccagtcttgg caaatatccc agcgattggt gatatcttct
    10981 tcaagtcaac tagtctactt ggttatctgg cgattgcctt ctcattcctt gcttggttta
    11041 ttctctttaa gactcaattt ggtcttcgtc tccgctctgt cggtgaacat cctcaagcag
    11101 cggatacctt gggaatcaac gtctacaaga tgagatattt agggattatt atttcaggtt
    11161 ttctaggtgg aattggcgga gcgatttatg cgcaatcaat ctcagttaac ttctcagtga
    11221 caactattgt tggacctgga tttatcgccc ttgctgcgat gatctttggg aaatggaatc
    11281 caatcggtgc tatgctttct agtctcttct ttggactttc acaaagtttg gctgttatcg
    11341 gttctcaatt gccgttccta caaggagtgc cagcggttta tcttcaaatt gcaccttatg
    11401 ttttgacaat tcttgtctta gcagccttct ttggaaaagc agtcgcacca aaagcagatg
    11461 gtatcaacta catcaaatca aaataagcat acaaaaaaac gtcagtccaa tgactgaact
    11521 gccccccaaa agttagacag aaaaaatcta acttttgggg tg
//
LOCUS       DAVKQL010000061        11514 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVKQL010000061 DAVKQL010000000
VERSION     DAVKQL010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11514
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.061"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            10..1246
                     /locus_tag="VW561_001734"
                     /pseudo
     CDS             10..1246
                     /locus_tag="VW561_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1374..2396)
                     /gene="cbpF"
                     /locus_tag="VW561_001735"
     CDS             complement(1374..2396)
                     /gene="cbpF"
                     /locus_tag="VW561_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpF"
                     /protein_id="HEU9943963.1"
                     /translation="MKLLKKMMQVALATFFFGLLGTSTVFADDSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDPLSGEMVVGWQYIPAPHKGVTIGPSPRIEIALRPDWF
                     YFGQDGVLQEFVGKQVLEAKTATNTNKHHGEEYDSQAEKRVYYFEDQRSYHTLKTGWI
                     YEEGHWYYLQKDGGFDSRINRLTVGELARGWVKDYPLTYDEEKLKAAPWYYLDPVTGI
                     MQTGWQYLGNKWYYLHSSGAMATGWYKEGSTWYYLDTENGDMRTGWQYLGNKWYYLRS
                     SGAMATGWYQEGSTWYYLNASNGDMKTGWFQVNGNWYYAYDSGALAVNTTVGGYYLNY
                     NGEWVK"
     gene            complement(2415..3236)
                     /gene="cbpG"
                     /locus_tag="VW561_001736"
     CDS             complement(2415..3236)
                     /gene="cbpG"
                     /locus_tag="VW561_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpG"
                     /protein_id="HEU9943964.1"
                     /translation="MNVEGRGSANFIKDNVLITAAHNYYRHDYGKEADDIYVLLAVSP
                     SQEPFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEKPIGAKLGTLGLPTSQKNLT
                     GITVTITGYPSYNFKIYQMYTDKKQVLSDDGMFLDYQVDTLERSSGSAVYDASHRVVG
                     VHTLGDGANQINSAVKLNERNLSFIYSVLKGYSLEGWKKINGSWYYYRQHDKQTGWQE
                     INDTWYYLDSSGKMLTDWQKVNGNWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            complement(3317..3556)
                     /locus_tag="VW561_001737"
     CDS             complement(3317..3556)
                     /locus_tag="VW561_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943965.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPGSNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(3706..3972)
                     /locus_tag="VW561_001738"
                     /pseudo
     CDS             complement(3706..3972)
                     /locus_tag="VW561_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(3997..4308)
                     /locus_tag="VW561_001739"
     CDS             complement(3997..4308)
                     /locus_tag="VW561_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEU9943966.1"
                     /translation="MDWDFADTCWEKDPREYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYEHSELEEIILILFENLFKPRQLYLQPQNSVLSNLRLA
                     S"
     gene            complement(4233..4475)
                     /locus_tag="VW561_001740"
     CDS             complement(4233..4475)
                     /locus_tag="VW561_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEU9943967.1"
                     /translation="MSLADLLEELEAAKDSKKARSMEAYMRHQFSFLGIAVPERNKLY
                     KNIFQKRKKQRLSIGILQTLAGKRILENTNMWLLTI"
     gene            complement(4476..5108)
                     /locus_tag="VW561_001741"
     CDS             complement(4476..5108)
                     /locus_tag="VW561_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9943968.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQDEF"
     gene            complement(5122..6117)
                     /locus_tag="VW561_001742"
     CDS             complement(5122..6117)
                     /locus_tag="VW561_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9943969.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSITCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQIQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFRLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(6114..6812)
                     /locus_tag="VW561_001743"
     CDS             complement(6114..6812)
                     /locus_tag="VW561_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEU9943970.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(6890..7900)
                     /locus_tag="VW561_001744"
     CDS             complement(6890..7900)
                     /locus_tag="VW561_001744"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEU9943971.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(7884..8891)
                     /locus_tag="VW561_001745"
     CDS             complement(7884..8891)
                     /locus_tag="VW561_001745"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEU9943972.1"
                     /translation="MIAVKTCGKLYLAGEYAILEPGRLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLEICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKAAAWLEEENLATVLERDWGFFISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLTSSKETVVSLVEALEQGKSEKIIEQVAVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(8878..9831)
                     /gene="mvaD"
                     /locus_tag="VW561_001746"
     CDS             complement(8878..9831)
                     /gene="mvaD"
                     /locus_tag="VW561_001746"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEU9943973.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMAFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(9813..10691)
                     /gene="mvk"
                     /locus_tag="VW561_001747"
     CDS             complement(9813..10691)
                     /gene="mvk"
                     /locus_tag="VW561_001747"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEU9943974.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVS
                     AESPWRLYEEDTLSMAVYASLEYLDITEACVRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(10813..11142)
                     /locus_tag="VW561_001748"
     CDS             complement(10813..11142)
                     /locus_tag="VW561_001748"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9943975.1"
                     /translation="MTRKVYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMLPHDFPLYLMVWSFFRRSMTTGWFQVNGRWYYAYSSGALAVNTTVDGYFVN
                     YNGEWVQ"
BASE COUNT         3469 a         2616 c         2038 g         3391 t
ORIGIN      
        1 cttaggtata tgattaaatt gattgatcgt tacggaatag agttcgtcaa aaaagggaaa
       61 aatcgttact attctcctga tttaaaacaa gaaatgagta ataaagtctg acatgaaggc
      121 tggactaaag atagagtttc tcttgaatac ggtctcccaa gtcgtacgat acttcttaac
      181 tggctagcac aatacagaaa aacgggtata ctattgttga gaaaccaaga gggagagtac
      241 ctaaaatggg acgtaagcca aaaacgagac ctgaagagag gacagaatta gaacgtcttc
      301 aagcagaaaa tgaatacctg agagcggaga atgccatcct aaaaaagtta agagaactcc
      361 gattgaagga ggaaaaagag aaagaagaaa gacagaaatt gttcaagaat taatgactga
      421 gttttcgtta gatcttcttc taaaagccat taaactagct cgttggacct actactatca
      481 cttgaaacag ctagacaaaa cagataagga ccaagagctt aaagctgaaa ttcaatccat
      541 ttttatcgaa cacaagggaa attatgctta tcgctgtatt catttagaac taagaaatcg
      601 tgcttatctg gtaaatcata aaagagttca aggcttgatg aaagtactca atttacaagc
      661 taaaatgcga cagaaacgaa aatattcttc tcataaagga gacgttggta agaaagcaga
      721 gaatctcatt caaggccaat ttgaaggctc taaaacaatg gaaaagtgct acacagatgt
      781 gacagaattt gccattccag caagtactca aaagctttac ttatcaccag ttttagatgg
      841 ctttaacagt gaaattatcg cctataatct ttcaacttca cccaacttag aacaagtaca
      901 aacaatgttg gaacaggcat tcaaagagaa gcactatgag aatacgattc tccatagtga
      961 ccaaggctgg caataccaac acgattctta tcatcgcttc ctagagagta agggaattca
     1021 agcatctatg tcacgaaagg gaaacagcca agacaacggt aggatggaat ctttctttgg
     1081 tattttaaaa tccgaaatgt tttatggcta tgagaaaaca tttaaatcac ttaatcaatt
     1141 ggaacaagcc attgtagact atattgatta ctacaacaat aagaaaatta agataaaact
     1201 aaaaggactt agtcctgtgc aatacagaac taaatccttc ggataaatta attgtctaac
     1261 tttttggggg cagtacataa gaaccttggt aatatgcgtt ttttgattgt gaagactttt
     1321 atccacctat tatacaaagt caagtatcca tcacagttta caattagcct tcattactta
     1381 acccattcac cattatagtt taagtagtaa ccacctactg tggtattaac agctaaagca
     1441 cctgaatcat aggcatagta ccagttacca ttgacttgga accagcctgt tttcatatct
     1501 ccattacttg catttagata gtaccaagtc gaaccttctt gataccagcc agttgccatt
     1561 gctcctgatg aacggagata gtaccacttg ttcccaaggt attgccagcc agttctcata
     1621 tcaccatttt cagtatctaa atagtaccaa gttgagcctt ccttatacca gccagttgcc
     1681 atagctcctg acgaatggag gtagtaccac ttattaccta gatattgcca acctgtttgc
     1741 ataatgccag ttactggatc tagatagtac catggagctg cttttagctt ctcttcatca
     1801 taggtaagag ggtaatcctt aacccaacca cgtgctagct ctccaaccgt caatctgttg
     1861 atgcgcgaat caaagccacc atccttctgt aaataatacc aatgaccctc ttcataaatc
     1921 caaccagttt ttaaagtatg ataactacgc tgatcttcaa aataatagac tcgtttctct
     1981 gcttggctat catattcttc cccatgatgt ttgttggtat tcgtagcagt ttttgcttct
     2041 aaaacttgct tgccaacaaa ttcttgtaat acaccatctt gaccaaaata aaaccaatct
     2101 ggtctaagag caatctctat tcttggagaa ggaccaatcg taaccccctt gtgtggagca
     2161 ggtatatatt gccagccgac aaccatctct ccggataaag gatcaaaata atagtacttc
     2221 ccatctatca ctctccagta ggtttctttt agatccccct ttttgtagta ggttctacca
     2281 ttttcttgga caaactgcca tccttcagaa tcatctgcaa atactgtact ggtccctagc
     2341 aaaccgaaga aaaatgtggc tagtgcgact tgcatcattt ttttcaaaag cttcaattta
     2401 tatatcctcc aacatcaaat ccactcccca gatgaagcga agttataaac tttaccatcg
     2461 atagtttggc tacctgtaac cattgctcca tttgaattga gataatacca gtttccattt
     2521 actttttgcc aatctgtaag catcttaccg gaactgtcta aataatacca agtatcattt
     2581 atctcctgcc aacccgtttg tttatcatgt tgtctataat agtaccaact accatttatt
     2641 ttcttccatc cttcaagaga gtaaccttta agaaccgaat aaataaatga caaatttcgt
     2701 tcatttaatt taactgcact gttaatttga ttagctccat ctcctaaagt atgcactcct
     2761 actacgcggt gactagcatc ataaactgca gatccactag acctctctaa agtatcaact
     2821 tggtaatcca agaacatgcc atcatcactt aaaacttgtt ttttatctgt atacatttga
     2881 taaattttaa aattatatga tggatagcct gtgatagtca cagttattcc tgtcaaattt
     2941 ttttgactag taggaagacc caaagtccct aattttgcac caatgggctt ttctagaatt
     3001 aataaagcca agtcatattc ccttgcatcc ttagaattta aatttctaaa ttccttcaaa
     3061 taacgaactt cctttacttt gatctttcca aatggttctt gacttggact aacagccaga
     3121 agaacataaa tatcatccgc ttctttccca tagtcatgtc tgtagtagtt gtgagccgct
     3181 gtaattaaca cattatcttt aataaaattc gcacttcccc tcccttctac attcattcca
     3241 tcggctactc cataatactt agataaaacc atagctgaag tcgaatacgg atactgtgaa
     3301 gtattatcaa ttttaatcaa atcatcatta ccgataatac ttctgattgc ttttggtagt
     3361 atgaaccata cgttggtgaa atctcagata atgaagaatc attagaccct ggaccttttt
     3421 ctagtgtctc acttacctca tattcttcac ctgtactaga aataacactc aaagcagata
     3481 ctgtcgataa ctggctagcc aataaagtac tcgcaataat tgaaataccc aattttttat
     3541 aaacagtttt cttcattatt gtatcctcct aatgtaatta tagcgtacta ttctaaattt
     3601 cttaatctac tatagaatca agaaatctac caccttcttt aaataccctc cattatcaca
     3661 taaacaggta aacttttcaa ttaatgactg cgcttttcaa tcacgctaga ggtacttgct
     3721 tgcttctttg atactaagtt cagccattct ttccttgttt ttctcaataa agcatgttac
     3781 ccaagtggga ttcgttttgg agtagtctcg cagagtccag ccaatggctt tattgataaa
     3841 aaattctgtt tggttcaagt tatgaagtag aatcttttcc attaattgag tattggtctt
     3901 ctcttttctt aacaactggt ggtcaatagc gacacgtctc agccagatat tatctgatag
     3961 gctccatttt atactcaatg aaaatcaaag agcaaactag gaagctagcc gcagattgct
     4021 caaaacactg ttttgaggtt gcagatagag ctgacgtggt ttgaagagat tttcgaagag
     4081 tattaagatt atttcttcta gttcagagtg ttcatacacc aaactcccta ctactcgatc
     4141 taggatatct accgtgtccc accaggattt tgtcacgact aactgctcta gcttaggcaa
     4201 atcggtttcc tttagataag actgcattgc tttcaaatag ttagcagcca catattggta
     4261 ttctctagga tccttttccc agcaagtgtc tgcaaaatcc caatcgataa tctttgtttt
     4321 tttcgcttct ggaaaatatt tttatagagt ttatttcttt caggcaccgc aatacctaga
     4381 aaagaaaatt gatggcgcat ataggcttcc atggaccttg cttttttaga gtcttttgct
     4441 gcttctagct cctcaagtaa atctgctaaa ctcatctaaa actcatcttg ccccaccaaa
     4501 tggtgctgaa aggcatagac agccgcctgg gtacgatcgc tgacttcaag tttggcaaga
     4561 atgttggaca cgtgggtctt gaccgtcttg agagagataa ataggtcatc tgcgatgcgc
     4621 tgattttcgt agcccttggc gatgagttgg agaacatctc gctcacgcgc agtcaattct
     4681 tcatgaagtt ccatatgatt gcggtggtat tcaaccttct tgctaacctc ttgctcaatg
     4741 gccagctcgc cagcagctac cttactgacg gcatgaagca attcatctgc actagaagtc
     4801 ttgagcatat agcctttggc accagcatct aagactggca tgattttttc attgtccaaa
     4861 taagaggtca caatcaaaat cttggcttca ggccattctt taaggattgc taaggtcgcg
     4921 tcaatcccat tcatctcagg catgacaata tccatgacaa tgacatctgg acgcagttcc
     4981 aaggccaagt caatcccttg agacccgttg gacgcctcac ccacaacttc tacatcgtct
     5041 tggaggtcaa agtagctttt caagcccaat cggaccattt catggtcatc tactagtaaa
     5101 attttcatct ttactccttt atcattcctt atctaacagg ggaatacgga tatcaactgc
     5161 cagcccttgc ttgggagctg ttaataactg aaccgtccct gccatatctt caacccgctc
     5221 cttgatattt cgcagtccat aactcaagtc gtctaagctc cctaaccgga aaccaatccc
     5281 attgtccacc accttcagtt gcaattcaac atctgtctga tagaggtaga catctaggca
     5341 agatgcctgg gcatggcgga gcgtattgct aatcaactct tgcagtatac ggaagatatg
     5401 ctcctcgatt ttcttaggca atttcgtcat attctgcttg agactaaccc taagatcact
     5461 cttgtcctca agctctttta agagaatctg aatcccttct atcaagctct tctgctccag
     5521 ttcaactggt cgcaaatgca agagcaaaac ccgcaaatcc ttctgggcag tttctaaaat
     5581 agctgtgaca ctctgcaact ggatctgcat cttttctcta tccaatttca aagcctgctg
     5641 actgataccc gataaaatca tgtgggccgc aaacaactcc tgactgactg tatcgtgcaa
     5701 atcccgagca attcgcttcc gttctttctc gatgatttcc tcttcctgag caaggctatg
     5761 attttcagct ttttgaagag cttctgtcaa aaggttaagt ttacctgata aggacttgaa
     5821 actggcatcc aaatctggat ctgcaacctg aaccacttct tgccctgcca ataaacgctt
     5881 gagattagcc tgcatttttc ttagagaaag ctcttcgatc cctcgccaaa acagggctaa
     5941 gagacaggtt atggacatgc tgaaaaccaa caataaaaag acaaactttt ctgttttttc
     6001 gacatcgtgc aaaaagatag accagtcaaa atcaagtatt tccagcaagc tgtgggagaa
     6061 aaaaaagaca aataggaagg aggtgagagc aataatgaca taggcttgtt ttttcatcct
     6121 ctaaccacct ccacatcacc aatcatagtg gtcaagaaaa tcttgacact cttgttactc
     6181 ttgagatagt cttttgtttc ttgatgatag tgttcattgc ggagggctcg cttgggctgg
     6241 ttgaaaaaag tcaaatcccc atagagacag ttaacgctga gactgacttc cacatctaca
     6301 ggtacgatga ttttggtcgt tcctaccatc tttctgagga taatgacatt gtcatgattg
     6361 gttaagatga ccctctccag atgaatagtg tccttgccca tgaagcgaaa gagattgata
     6421 tcatcgaatt ggcaagtctg gtagcttgaa aaatgatgaa gatttccaaa ccaacgattt
     6481 ttctccttct taaccgtcac gacctcttca aaaaccaaat tggtctgctc tttttcctgg
     6541 ttcatcatcg ggtaaagaag aaagaggcta tagataaccg caacaaaaat agctagaatc
     6601 acaaaaggat tgagcataac gatgaaaaag aagagaatgg ttgccgctac taaaagaaga
     6661 ttatttccct ctttaccagt gtagtagcga atcaaaagca aaaagaggaa tagtatcagc
     6721 agaaaacgcg aaaaatgctc tgataccatc aaaatcagag ctcctgtcag aagacaggct
     6781 tcgataaata aaaagatttt aaattttctc ataggttcat cctctccctt ctattttatc
     6841 acaattcaaa aaagtcacct cagtctgagg atggaaaaaa aggcgctggt tacgcctttt
     6901 tcatctgatc ctttgcttct tttaattttc cataaagaag atagtctact ttttgtagat
     6961 ctgctatggt ggcacagtta agggaacaca taatcaagcg tagatctgct ttccagcctt
     7021 ggacaatgcc aatcacttct tcaactgtgt aggtttcaac caattccaga acggttcgtg
     7081 acaatcccac agccttagca ccaaaaacca agcacttaat catatccagc ggattccgaa
     7141 cccctccact aaccaagagt tcgaccttat ctttccattc ttgggcattg agaagggcct
     7201 gcatggtaga ctgaccccat tgattgaggt aatcacgctg gccactacga cggttttcga
     7261 tataggcaaa gctggtgcca ccacgacccg ataggtccac tgtacgaaca ccgaattcat
     7321 aggctctttc gattgtcttg gcatccattc caaagcccac ttccttgagg acaataggaa
     7381 cgggaatttg cttgctataa tctgctaaat gcgattgcca gcttctaaac ttcctttctc
     7441 cctcgggcat gagtaattcc tgcatgacat tgacatgcac ttgcaataga acaggattca
     7501 tctcttctac agtctgaagt cctaactcga caggcttgtc caatccaata ttggttccaa
     7561 ggaggagatt gggatgacta gacttgacag aaaaagaatc atccgttgga tttttgaggg
     7621 ctgcgctata agaacccgtt acaaataaaa taccacagga ttccgccacc tgagccagct
     7681 tttgattgat ttctcttccc ttattacttc caccagtcat ggcattgata taaaaaggaa
     7741 agtcccactt tcgaccagca aactctgtcg aaagatcgat ttcatccaga ttgtaaagag
     7801 gcaaggaaga atgaatcagc tccacctcat caaagctatt ataggaactt ttctgctcaa
     7861 gggcatagag gatatgctcg tccttacgat ttgtcgtcat gtcctatcct ttcttgatat
     7921 aagagctcaa tccccagatc ggcccaacga ttttttaagg ttttggttga ttgcgcatca
     7981 aaactcaggg cgatgccaca gtcaccacca ccagcaccac tactcttggc aacggcctgc
     8041 aaatcttgac tggcttcttt caactgtcta agcaaaggcg tgtaaatatc tgtactcaag
     8101 ccttctaaaa gcttgctggc tactgctact tgctcgataa tcttttctga tttcccctgt
     8161 tccaaggctt cgaccaaaga aaccaccgtt tcttttgagg aagttaaaaa attttgattg
     8221 atattttgct tgatttgctg aaccatgtga ctcgatacag ccacttcctt ggtccatccc
     8281 actaagaaat cacattctaa agttggtttc acttgtgaga taaaaaatcc ccaatcacgc
     8341 tccagaactg tcgccaagtt ttcttcttct aaccaagcag ccgccttctg gcgatcaaat
     8401 gactggtaga gaaccaaatc ctctgccaca atacaggcaa ggtcgcccat ggaaccattg
     8461 tctcctcgct tgagcaagac agcgctagtc agcttgaaca agagctcctg atcaacagaa
     8521 acatcataca gagccagtaa agccttgaca accaagacaa cgacgctgcc actagaacct
     8581 agaccaaact ttttcccttc tcgttccatt ttgccacaga tttctagaga aaaaggtctt
     8641 aaattctgac cgcgaacagc gaggaagtct cccatcaaag caatcgtttc ttgaatcaag
     8701 ctgtagtcag gattgggcct taagtccact gcgaaatcaa acatatctga atagatacgg
     8761 tagctgtcag aaaaagcaat ctcagccctc atatagatgg gaatatcctt tatcaaagct
     8821 aaccgccctg gctctaaaat agcatattca cctgccaaat agagttttcc gcaagtttta
     8881 acagcaatca tcttgactca aatcctttgt ttttgacaca atcaagcgat aacgctgacc
     8941 gaaaatttct gacaaatgct ccaagtcttt ctcctgacag aagaccttaa cattgggacc
     9001 agcatccatg gtaaagtagc aggcctctcc tttctcacga agctgacgaa caaaggccat
     9061 agcctcataa gaggcatccg tcagataaga aaaggctgga ctagcagtct ttgtcgtagc
     9121 atgcatagcc agggcatttt tctccgttaa ttctccaatc ttggcaaaat cattttcctt
     9181 gagataaatc agcatatcct gatagtcctt ctcagactga cgaacccagt cgtcgaaagt
     9241 cgtcgaggtt tccacacaaa gtttcatccc gtcacggcta gagattggtt ttttcttgtc
     9301 ctctagcacc aacataatca tagctagttt caagtctgtc tctacagggt aaatttctcc
     9361 actatcctta tcccaggctc ctagtggtcc ataaaaactc cgagaagaag agcctgaggc
     9421 aaatttggct tcctgtgcca actgacttct atccaatcca agtttgaaat aagcattaca
     9481 agccttgacc agggcggaca aaccactaga acttgaggac aggcccgctg ccgtaggcat
     9541 attgttttga gtatcgatac ggacaaagcc ctcaccagct ggacgataac ggtcaataat
     9601 cttactcatc ttggcatgct cgacctcatt ttgtagctga ccattgatgt aaaattcgtc
     9661 agctgttaca ttggctggta aaggcgacaa ggtcgtctct gtatacatat tttccaaagt
     9721 tagagaaata ctgctagtag caggcaccat ctctttttct tttttctttc cccaatattt
     9781 gataatagca atatttgcgt aggaacgtac tgttacaggc tctctatcca tgtctgaaca
     9841 gctcctttct cttctaatct ttctgctagt tcttgtgcgt gtgtcaaatt ggttaccaag
     9901 gctatgatac aacctcctag cccaccaccg ctcatcttgg cacccagagc accatggcta
     9961 agagtcgttt caaccaaaaa gtctgcctca gggctactga ctccaatttc ttttaaatgt
    10021 aaatgcgctt gactgaggat ttgtcccagt ccttcagcat ctttttgtga aatcgcaact
    10081 tctgcttgct gggttaattc tcccaaggca tgcaaaaacg gtagggcatc cttgccctta
    10141 ttttgaacca cttggatggc ttcacgagta tgaccataaa cacccgtatc ggcaatcacc
    10201 aaataggcgg ataaatccat ctcaagttct gtaaatccta cgttcttgat aaagcgaata
    10261 ggttggtcac tgagacaggt cttagcatcc aaaccactag gattcatatg ggcaatcatc
    10321 tcagctcgat tgaccaagat ttctagtaca tcatgaggca gatcagcctg atagtagtca
    10381 aataccgcac gaatggccgc tatgctgata gccgctgacg aacccatccc ccgtttctca
    10441 gggatagccg agtcaatctc acaacgaacg caggcttctg tgatatccaa atactccagt
    10501 gaggcataaa ccgccatgga caaggtatcc tcctcataaa ggcgccaagg actctctgca
    10561 gaaactacct tacaggtcac ctccacctcc aaaagaggca gggaaatggc aggataaccg
    10621 taaacgaccg catgttcccc tattaaaatt atcttactat gtgcctgacc gacaccaact
    10681 ttttttgtca ttttttcctt ttactagacg aaaaaacgtc ttatttttca tacaagtatt
    10741 aattctttcc tatctatttt attatatttt cacaaaaaaa gcgattgttt ccattcacaa
    10801 tcgcttcttt cattattgaa cccattcgcc attataattg acaaaatagc catctacggt
    10861 cgtattcact gccaaggcac ctgagctata agcgtagtac catctgccat tgacctggaa
    10921 ccaacctgtc gtcatagaac gacgaaagaa actccatacc attaagtaaa gaggaaagtc
    10981 gtgagggagc atgcgccatt gacaacctgt tttagtgacg tacaaagtct cattaacaag
    11041 tactcgtttc ggccatttat aggtgcggtg tttggagaaa tagggttcaa tcttagccca
    11101 ttcttgatcg tttaaatcag tatcatatac tttgcgtgtc ataactctag tttaacattt
    11161 tttgtgaata caggttctaa ataatcgacc acgaaaattt cttaagtgga aaacgcctta
    11221 tgaagtatgc tacgggaaag ttatgcactt aatttgacaa ttcaagatgt aaaaatatat
    11281 actatagtag attaaaacta gaatagtaca cctctacttc taaaatattg ttagaaatcg
    11341 atttgactgt cctgatcgat ttatcctgtt attatctcat tttgctataa tatttgataa
    11401 gttatcctaa aagtattatt atgttgttgt gttatagatt gattgaatct aactaaagga
    11461 tcctattcaa ttactagaac tatcacatac tcaaggtcag ctcacagatg agca
//
LOCUS       DAVKQL010000062        11230 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVKQL010000062 DAVKQL010000000
VERSION     DAVKQL010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11230
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.062"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            166..351
                     /locus_tag="VW561_001749"
     CDS             166..351
                     /locus_tag="VW561_001749"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943976.1"
                     /translation="MDRTDEVSSKHRFEVADRTDQVSSKHRFEVADRTDQVSSKHRFE
                     VADRTDEVSNIYTARRS"
     gene            complement(707..1687)
                     /locus_tag="VW561_001750"
     CDS             complement(707..1687)
                     /locus_tag="VW561_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU9943977.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            complement(2004..4019)
                     /gene="recG"
                     /locus_tag="VW561_001751"
     CDS             complement(2004..4019)
                     /gene="recG"
                     /locus_tag="VW561_001751"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU9943978.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKYLAEYKQALRRIKFEELFYFQMQ
                     LQMLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            complement(4038..5141)
                     /locus_tag="VW561_001752"
     CDS             complement(4038..5141)
                     /locus_tag="VW561_001752"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU9943979.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVCVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKLYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            complement(5131..5499)
                     /locus_tag="VW561_001753"
     CDS             complement(5131..5499)
                     /locus_tag="VW561_001753"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEU9943980.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            complement(5538..6569)
                     /locus_tag="VW561_001754"
     CDS             complement(5538..6569)
                     /locus_tag="VW561_001754"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU9943981.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            complement(6571..7602)
                     /locus_tag="VW561_001755"
     CDS             complement(6571..7602)
                     /locus_tag="VW561_001755"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU9943982.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            complement(7683..10196)
                     /gene="secA"
                     /locus_tag="VW561_001756"
     CDS             complement(7683..10196)
                     /gene="secA"
                     /locus_tag="VW561_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEU9943983.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYTQRYDVITADRDL
                     APEIQAMIKRTIGRVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            complement(10342..10629)
                     /locus_tag="VW561_001757"
     CDS             complement(10342..10629)
                     /locus_tag="VW561_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943984.1"
                     /translation="MLNLKSFITRYSKVYIGLVLLIWIAFFFLPWGSPILGGKIDLFS
                     IQKVLLVFGILSILMALLSKKVSLFVFGLICCLSLWINLFILFAILPILGN"
     gene            complement(10623..11198)
                     /locus_tag="VW561_001758"
     CDS             complement(10623..11198)
                     /locus_tag="VW561_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HEU9943985.1"
                     /translation="MKTIVKICLVALSAFLLLFAGKNVFADSNSHDNLNSLEVSDRSF
                     RNGELLKSYLVKDKDRIYFDYDKAVQDKQAKDVIDMGLLVETISEDYSSNTFTPAKYF
                     KAGLPVHGNYCGPGYNGEGFTLPVVDVLDQGCQNHDRCYKWGAGIGANCECNRQLVDF
                     IKVNRRWIPESALWVADAIRVYFETIGAIGC"
BASE COUNT         3194 a         2646 c         2154 g         3236 t
ORIGIN      
        1 tttcaaaaaa ttcttttata taataatgtc cttgaaagta gtttcagctg aacatttata
       61 gtccataaaa agaaaacaga aactcatctg tttttatact caatgaaaat caaagagcaa
      121 actaagaaac tagccgcatg atgctcaaaa cactgttttg aggttgtgga tagaactgac
      181 gaagtcagct caaaacaccg ttttgaagtg gcagatagaa ctgaccaagt cagctcaaaa
      241 caccgttttg aagtggcaga tagaactgac caagtcagct caaaacaccg ttttgaagtg
      301 gcagatagaa ctgacgaagt cagtaacata tatacggcaa ggcgaagctg acgtggtttg
      361 aagagatttt tgaaaagtat taacacctac agacaagcct gacgatagca agaactaccc
      421 tactcgatag gtatcggctt ttgctttctg aaaaaaatta ttttaagcaa tttgacaaat
      481 ctagcaacaa aaaattctat aaatataata tagattgaaa ctagaatagt acacatctac
      541 ttctaaaaca ttgttagaaa tcgatttgac tgtcctgatc gatttgccct gttcttgttt
      601 cattttacta tatttccatg ataaaacgca tagtatcaag ttttcttaag cccctgatac
      661 tatgcgtgtt tgtaattttt aagattttgt gcttagagtc gactccttat tttagatatt
      721 taaaaggaat ctcacttcca cagagccagt tgtagacttg gtcattgaca aatacattca
      781 tggcttcgtg agcatactca ggcatgatgc gataggtttt atcgcaggtc agacgattat
      841 aaatcgcaaa ctgggtaatg ggatagcaaa catcgtcgtc caagcccgta atcatcttaa
      901 cctcaccttg gatacgatgg gcaagatttt tgacatcgat ataggcaagg gtcgccatga
      961 tttcctcctc tgtttcatgg aaggggtcgt gaaacttgaa ataacggaaa agttcgtcgt
     1021 aagcctcgct agtattacca atctcaatca cccgtctgaa gtctgacaag aagggataaa
     1081 tggcaactgt tttctgaatt cgaggattga gcgctgctgc aactagagct agagcccctc
     1141 cttgtgaggc accatagcta gaaagacgct tctcatcaac ctgagacaga ctagcaacaa
     1201 tttcgaccaa ctggtaaata tccagataaa catccttata aaagaggtgg tcccgacctt
     1261 ccatagcacc acggataata tgccccttca cggtatttcc taaaggagaa cgcaagccgt
     1321 cttgtgagta acctgactgg ccccgcacat ccatggaaac aacaccgtaa ccagctacgg
     1381 tgaagcccag catgtcggcc cagtcccagc cacgtcccat ataaccatga aaatggaaga
     1441 ttagtggaac cttctcctca ctctttggaa gaacaatgcg tgcatagacc tttccttcct
     1501 tgcttccttc aaatgttaac tcatagcact tgacttgagg aatgtggaaa tctctttcct
     1561 ccaagtggta ggatggaagc gtggaaacat ttttcacttc cccatcccag aaagcatcaa
     1621 agtcttccgg aacctcatcc cttcctctat aggtcttaat ttcttctagc aaagctggat
     1681 ttttcatagc atgctccttt tattttgtaa tcgttaccac aactgtagca tatctatgta
     1741 atcaatgcaa gaaagaaggg taaatagaaa gtgaatttag attctttact tttaggatga
     1801 tgggctgaaa gtagttttag aaacgaaaaa agagcatttc gctctctctt ctgtttatac
     1861 tagttttttg cttccttctt ttggaaagtg gtttggtaat ttactttaat cgttactgtc
     1921 atatgagagt cctcgtttct ttttgatgac tctagtataa gagccaaacc taaaaactag
     1981 cttaagtttt ccttagagaa agcttaatcc agatgttctt tcttttccag atgaagggca
     2041 atcatgcgcc actctggatc ttcttgccaa gcttctatag aactaatgta gctagcaacc
     2101 tttcttgctt cttctaaaat cggaaaatct tcgataatat cagccacttg gaactctgga
     2161 agtcctgact gtctggttcc aaaaatctca ccagaaccac gcattttcaa atcttcctcc
     2221 gcaaggacaa atccattggt cgtttctgtc atgatgcgca tgcggtcttt cccagaatcc
     2281 gtcttgggat tggcaacgag aacagcgtag gactgcttgt ccccccgacc gacacgacct
     2341 ctaagctggt gaagttgact gagaccgaag cgatcggcat ccatgataat catgacagtc
     2401 gcattgggaa cgttgacccc aacctcaata accgtcgtcg aaaccagaat atccgtcttt
     2461 ctctccttga aatcctgcat gatctggtct ttttcgtcac tcttcatcct accatgtaga
     2521 agagccacct ctgccttgcc tgcaaaatga gtcgtcaact cctctgataa ggcaatggca
     2581 tttttcaaat ctagagcttc tgattcttca atcaaaggag agatgacata ggcttgggaa
     2641 cctttttgaa tttccccctc taaccaagtc aagacctgag gtagttgctc atgtttgatc
     2701 cagcgcgtca caataggctt ccgacctgct ggcatctggt cgataatgga aacatccata
     2761 tctccaaagg ctgtgatggc aagcgtccgt ggaatgggag tcgccgtcat catgaggaca
     2821 tctggattgt cacctttttc ccgtaaaata cgcctttgcc ctacaccaaa acggtgctgc
     2881 tcatcgataa taatcaaacc aagacgagca tactccaccc catcttgtat cagagcgtga
     2941 gttcctataa tcaaatcagc ctcacccttg gcaatggtct ccaagacttc tctcttttct
     3001 gcagctttca aggaacctgt caagagagcc agtttcaaat tgggaaaaag gttctgtaaa
     3061 ctctcaaagt gttgctctgc gaggatttct gttggtacca ttagggcagc ctgataacct
     3121 gctgtcactg ccgcaaacat ggccaagcca gcgactaccg tttttccact ccccacatcc
     3181 ccttgtagga gacgattcat gtggtggtcg gacttcatat cagttaaaat ttcctgcaaa
     3241 ctcttttcct gagcttgggt cagggcaaaa ggaagactta ctttaactgc tgtcactttt
     3301 tcctgagacc aattcagaac cagaccactt ccctgaactc tattttcaga cttgagcatc
     3361 tgcagctgca tttggaaata aaagagttcc tcaaacttta tacggcgaag agcctgcttg
     3421 tattctgcca aatactttgg aaaatgcata gcacggactg cctgacaacg ggacatgagt
     3481 ttgtatttgt ctagtaaaga ctggggcaga ttttcttcta tcaagaggtc cagtccctga
     3541 tcaaaagccg tcttgatgac cttgaccaga ctggcctgac tgattccctg agccagacga
     3601 tagacaggct ggaggtcatc ttctacctga gccagaacct tcatcccagt cagactagcc
     3661 ttagcgcggt cccattttcc aaagacagca agggttgctc ccaactctat tttatcagcc
     3721 agatagggct ggttaaagaa attcaccgca aaaacgacct ctccctgctt gagactaaaa
     3781 cgcaggcgat tgcgcttgaa accataatac tggacactag caggagtcac tacctgacca
     3841 gaaagaactg ccttctcacc gtcttccagc tccagcacct gcttggtttt gaagtcttca
     3901 taacggaaag gaaagtagag caagagatct tgcaagtttt caattcctag tttggcgtat
     3961 ttttctgctg actttggtcc cacaccaggc aagacatgca agggttgatg tagattcatg
     4021 ctccactcct ttcttttcta ataatattct ctcggaatac ggtcgctgag gaggcaaacc
     4081 acctcatagt taatggttac gcggtaggtc gctacctgag ttgcagtgat ttccttatcc
     4141 ccattggagc caatcaaggt taccttggtt cctagcggat aaagcttagg caatcgaata
     4201 gtgatttggt ccatcgaaac cctgccgaca attgggcaag cttggccatc taccaagaca
     4261 gagaaatttt gcatgtctct tgtccatcca tctgcatacc cgattggcac ggtcgcgatg
     4321 acttgctcgc tatccgcttg ataagttgct ccatagccca tgcaagctcc agctggaact
     4381 gtcttgacat gaaccagagc agactccaag gtcaaggccg gtatcaaatc ataaggcaaa
     4441 tccaagaccg ctccacttgg attgaggcca tacatggcat ctcccatacg aaccgcattg
     4501 aaaatagtct ctacatgcca aagagtcgtt gcagaattgc tagcatgaac cagctctgga
     4561 acttccttca tactagctaa aatagtttta aaccgttcta actgggcatt aaaatagtca
     4621 tctgattcct catcagcagt agcaaagtgg gtaaagattc cttcaacaca aacaccgtgt
     4681 tgttggagca aatcttgagc ctgctcaacc tcacttgcct ctctaaaacc aatccgtccc
     4741 atccctgaat caatcttgag gtggactgtc aatccagtta ggtccacttc cttatctaag
     4801 agtgcttgaa tccactccag tccagccact gtcaaggtga agtcatattc tttagctaga
     4861 gcaacagctt cgatttcaga aactcctaaa atgaggattg gcttgctgag tccagcttgt
     4921 ctgagttcaa tggcttcatc gatattggaa acgcaaaagc catcaacatc atcttgaatt
     4981 gccttggcaa cggcaacagc tccatgacca taagcattgg ccttaaccac agccaacttg
     5041 agcgttcctt gagggatatg agcccccatt tgctgaatat tttgtcgaat agctcccaga
     5101 tgaatcagag ccttggttgg tctatgtgga ctagctttca tgattttcct ccaaaatgac
     5161 actggctgtc acaaactgat cggtgtggct gatagacagc caaatctttc ctgaaaatgg
     5221 tgcctgacta aaataaggcg ccccacgttc attgttcaag acttccaaat cctgaaaacc
     5281 gagcttgcta atgcccgttc ccatagcctt ggaaaaggcc tccttagccg accagcgacc
     5341 agctaaatat tctatttgcc tgcgtccttt gagactggtg aagcgctcca tttcctgagc
     5401 ggtcagtaca cgcttagcaa atccttcatg tcgtgtaact gcgctttcta tcgaagccaa
     5461 ttcttcgatg tcaattccgt gtccaactat cattctcatc aaaaggagtt gagattcccc
     5521 aactccatcc ttttcactta ttttgtcaag gtaacataaa tctcttctac caaggcctct
     5581 gtattttccc aacctaggca agggtcagta atagagcaac caaagatctc tggttggttt
     5641 tgacgaccat ctgctaggta agattcaatc ataaatcctc gaaccgtctt tttaattttc
     5701 tcattccaat cacgattctg caaggtctgg cgaacaattc gaatctgctc catatattgc
     5761 ttgcctgagt tatcatggtt ggtgtcaatg aggataaaag gattttcaag tcccatggtt
     5821 tcatagcgtt caatggcttg gagtagattt tcatagtagt aattcggcat ataattgcca
     5881 tactcgttga ctgctccacg gaggataaca tgggccaaag gattacctga tgtctcaact
     5941 tcctgcccat gataaaggaa ggtttgcttg ttttgagcag catagatggc gttaaacata
     6001 acacccaaat ttcctgaggt tggatttttc atccctactg gtgcatcaat cccagaagcc
     6061 acaaagcggt gctcttggtc ttccacagaa cgagctccaa cggcatggta gctgaccaag
     6121 tcatccacca agatcagatt tgacggataa agcatctcat ctgccgttgt caaaccagtc
     6181 tctgtaatca cgcggtagtg caactggcgc acagcctgca agccattaat caggcttgga
     6241 gccttagaag tatctggctg gtgaactaat cctttatagc cgtctccatt ggtacgaggc
     6301 ttagcagtat acacgcgcat gaccatgaaa atcttatccg ctaccttctt ttgcaaggca
     6361 gataaacggc gagcatattc caagaccgcc tcttcattat cagaagagca aggaccaatc
     6421 accaagagaa tacggtcatc ttcccctgaa ataatatctg ccaattccct gtcacggctt
     6481 tccttgagtc tcaaggcttc cgcagacaat tgggtttcag ccttgatgac ttccatatcg
     6541 atttcttgac ctttttcaat aaatgccatc ttattctcct aacgttttgt aaatttctct
     6601 gacaagagct tctgtgttat cccaacccag gcaagggtct gtgatagact taccaaatac
     6661 ttctggctca ttttgtcgac catcttccag ataagactca atcataaaac cacgaacgaa
     6721 ctgcttaatt ttttcattcc aagcacggtt aatcaaggtc tggcggacaa ttcggatctg
     6781 ttcaatatac tgcttaccag aattgtcatg attggtatca atgatgataa aaggattttc
     6841 caagcccatt ttctcatact gggcaatggt atcaattaaa ttgtcataat agtagttggg
     6901 aatatttttt ccatactcat taagagcacc acgaagaata gcgtgtgaaa gcgggttccc
     6961 agttgtttct acttcttttc ctaagaaaag gaaactttgt ttgttttgag cagcataaat
     7021 cccattaaac atgacattga gatttccaga ggttggattt ttaaaaccag tagaaaatcc
     7081 tgccccactt gccacaaagc ggtgttgctg gtcttcaact gaacgggcac caactgccat
     7141 gtaagaaatc aaatcatcta caagcggaag gttttcagga taaagcattt catcagcagt
     7201 tgtcatccct gtttctgtga tgacacgata gtgaagatgg cgaacggctt tgattccatt
     7261 gataagacta ggcgcttctg tcgcgttagg ctggtgaatc aagcccttat agccatctcc
     7321 gttggtacgg ggtttggcag tataaacacg cataaccata aagatacgat ctgccacttc
     7381 ttcttgtagg actgccaaac gcttagcgta ttcaaggaca gcttcttcgt tgtcagatga
     7441 gcatggcccg attaccaaga gaattcgctg gtcttctcca cgtataatgg cttctagctc
     7501 ttgatctcgc tgtgatttcc tctccaaagc ctgaccttct aattttgaca aggcacgaac
     7561 ttcttctata tttattttag agctttttgc tgtaaatacc ataactcatc tccttataaa
     7621 gcaaacggat acccagtaaa aatttacttt tcacaagata tccgcctcta aactatctca
     7681 ttttattgtc ttttaccgtg acagttttta aatttcttac cagaaccaca tgggcaaagt
     7741 tcattgcgtc caatctggct caaatccaaa tcttctggca tacttgcttg gtgagcagcg
     7801 atattgcgag tcgctgttgt actgatatga cgttctgcct gtggtctttc ttgttcatga
     7861 atttgtgctt tcatcatcaa gcgtgtcaca tcaaactcaa tcgaaccaat catatcatta
     7921 aacatacgga aaccttctgc ctgatactca acaacagggt tgttctgagc atagccacga
     7981 agtccaaccg cgttacgcaa ttgatcaagg gcatcgatat gatctgtcca cttgttatcc
     8041 accactcgta gaatcaaaac tttttggaat tctttaactg cttcttcatc gcgtagtttt
     8101 gaaacctgac tatcgtaaac cttcaaggca cgttggaaaa gctcttcctt gatggcctta
     8161 tcagacaagc ctgacaagtc ttccatcgta atagaatctt caggaagcaa gttatactta
     8221 gcgaagttca aaattgcctc tagtttctca tcttgtttag cacgcgcatg accgtcaacg
     8281 acacgaccaa tcgtgcgttt gatcattgcc tgaatttcag gtgccaagtc acggtctgca
     8341 gtaatgacat cataacgttg agtatagata atctcacgtt gttcacgcat gacatcgtcg
     8401 tattgaagga cttgtttacg ggtatcgtag ttatttcctt cgacacgttt ctgagctgct
     8461 tcaacctgac gcgtcaacat gcgagactca atcgcctctt cagacatgtt caagcgttca
     8521 aagattccct tcaagcgttc agaaccaaaa cgtttcatca aatcatcttc aagagatagg
     8581 tagaattgtg actcacctgg atctccttga cgacctgaac gtccacgaag ctggttatcg
     8641 atacgacgac tttcatgacg ttctgtacca ataacacaaa gtcctccaag ttcacgaaca
     8701 ccttcaccaa gcttgatgtc ggtaccacga cccgccatgt tggttgcgat ggtaacggca
     8761 ccacgttgac cagcattcat gatgatttgg gcttctctat agtggttttt ggcattcaag
     8821 acttcgtgag gaacaccagc tgcaaccaat ttcttagaaa tgtagtcact agtttcaacc
     8881 gctactgtac caaccaagac aggttgaccc ttttggtaac gagccttaac gtcttcgaca
     8941 accgctttaa acttagattc tatacttgca taaagaaggt ctgagtggtc aatacgttga
     9001 acaggacggt ttgttgggat tggaataaca cgaatgttgt agatttcacg gaattcttct
     9061 tcctcagtct tacctgtacc cgtcatacca gacaatttct tgtacatacg gaaaaggttt
     9121 tggtacgtga ttgaggcaga tgtcttggtt tcatcctgga ttggcacacc ttctttggct
     9181 tcaatagctt ggtgcaatcc atcagaataa cgacgacctt ccatggtacg acctgtaaat
     9241 tggtcgacaa tcaagatttc ttgctcttcg ctcaccacat agtcaatatc gagaagcatg
     9301 atgtagttgg cacgaagggc gttatcgata aagtgggtca aagccacgtt ttcgatgtca
     9361 tagaggtttt caagtttgaa gtagctttca gccctgtcaa tccctgaatc agacaaacca
     9421 atagtcttag actgcacatc gatgatgtag tcatctttgt tcaaagattt tacatagtgg
     9481 tctgccatgt gatacaactg actggtttca accgcattgg cacctgatac aatcaaaggt
     9541 gtacgagcct cgtcaatcaa gatagagtca acctcatcga ccaaggcata gttaagcgga
     9601 cgttgtacca tgttttcagc gcgaacgacc atgttgtcac gaaggtagtc aaatccgatt
     9661 tctgagttag ttgagtaagt aatatcacac tcataggctt cttttttctc cattggagat
     9721 ttggtagcca agttaatccc tactgacaaa ccaagccaag agtacaattc acccatctca
     9781 gtcgcgtcac gttctgacag gtattcatta accgtaacta cgtgaacccc tttacctgaa
     9841 agggcattga ggtataccgg catggtcgca gtcaaggttt tcccttcccc tgtacgcatc
     9901 tctggcacgt caccatggtg aagaacaatc ccccccatga cctgaacctt ataagggaag
     9961 agacctagga cacgtttggc accttcacgg acaaccgcaa atgcttcgta aagcaatgaa
    10021 tccagtgatt ctccattttg ataacgttcc ttaaattcaa ctgtttttgc ttttagttgg
    10081 tcgtcagtca aagcagccat ttggtcttcg tatttgaaaa ccttgtcagc catcttttcc
    10141 agacgacgga tttctccttt atcattttcg ataattgttt ttaaaatatt agccatgttt
    10201 ttccttactt taaattccga atattttaga atgttccttt aattttagca caattactga
    10261 ttattttcaa ggaagaatcc ccattttgaa aaggaaaaag aggttagttt ccaagctaac
    10321 ctctctgact tttatgatac tttaattgcc aagaatcggc aaaatagcaa ataggataaa
    10381 taagttaatc caaagagaaa gacagcagat caatccaaaa acaaagagac tgactttctt
    10441 ggacagcaag gccatcaaaa tggacagaat gccaaaaact agcaagactt tctgtatgct
    10501 gaagaggtca atcttccccc ccagaatcgg actgccccaa ggaagaaaga agaaagcaat
    10561 ccagatcaac agaactaaac caatatagac cttagaatag cgtgtaataa atgattttag
    10621 attcaacatc ctatagctcc aatcgtttca aaataaactc tgatagcatc cgcaacccat
    10681 aaggcacttt ctggtatcca acgtctgtta acttttataa aatcgactaa ctgacgatta
    10741 cactcacaat tagctccaat accagctccc cacttgtaac atctatcatg attttggcaa
    10801 ccttgatcca aaacgtctac cacaggtaaa gtaaatcctt ccccgttgta accaggacca
    10861 cagtaattcc cgtgaacagg caatcctgcc ttaaaatatt tagcaggtgt aaacgtattt
    10921 gaactatagt cttcgcttat tgtctctacc aataaaccca tgtcaattac atctttcgct
    10981 tgtttatctt gaaccgcttt atcataatca aaataaatcc tatctttatc ttttacaaga
    11041 taactcttca acaattctcc gttacgaaaa cttctatcgc taacttccaa agagtttaag
    11101 ttatcatgtg aattagagtc tgcaaaaaca ttttttccag caaataaaag taaaaaggca
    11161 gataaagcaa ctaaacaaat tttaacgata gttttcatga ctactcctcc taataaacat
    11221 aagttataag
//
LOCUS       DAVKQL010000063        10968 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVKQL010000063 DAVKQL010000000
VERSION     DAVKQL010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10968
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.063"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            11..733
                     /locus_tag="VW561_001759"
     CDS             11..733
                     /locus_tag="VW561_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU9943986.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
     gene            730..1578
                     /locus_tag="VW561_001760"
     CDS             730..1578
                     /locus_tag="VW561_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU9943987.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            1610..2539
                     /gene="psaA"
                     /locus_tag="VW561_001761"
     CDS             1610..2539
                     /gene="psaA"
                     /locus_tag="VW561_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEU9943988.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            2662..3153
                     /gene="tpx"
                     /locus_tag="VW561_001762"
     CDS             2662..3153
                     /gene="tpx"
                     /locus_tag="VW561_001762"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEU9943989.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(3249..5957)
                     /locus_tag="VW561_001763"
     CDS             complement(3249..5957)
                     /locus_tag="VW561_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU9943990.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMETTANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEELEQILSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEAKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLL
                     ANVISSVITKGMVVGETQIQFYWTYSLLALVLSLLASVLPAYLVAWRELHDEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKAL
                     DKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVATVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(5959..6660)
                     /locus_tag="VW561_001764"
     CDS             complement(5959..6660)
                     /locus_tag="VW561_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9943991.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPAQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            complement(6910..9321)
                     /locus_tag="VW561_001765"
     CDS             complement(6910..9321)
                     /locus_tag="VW561_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEU9943992.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGLI
                     GSERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALATTSYVYKGTRFE
                     REAFASFPDDLLVQCFTKEGLETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLETVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDENLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAARASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKISTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            complement(9374..10066)
                     /locus_tag="VW561_001766"
     CDS             complement(9374..10066)
                     /locus_tag="VW561_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEU9943993.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            complement(10601..10957)
                     /locus_tag="VW561_001767"
     CDS             complement(10601..10957)
                     /locus_tag="VW561_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9943994.1"
                     /translation="MSGIVGLVGGILLLIGPFVLLGTTVNTAATTLNGGATAGAFSGV
                     ALLLNALKIENLVLGIIAIVYYKGDKRVGAAPSVLMIVSGGVSLIPFLGWVGGILAII
                     GGSLFLATLKKFKSEE"
BASE COUNT         3209 a         2517 c         2050 g         3192 t
ORIGIN      
        1 atacaagttt atgatacgta tcgaaaacct cagtgtctcc tacaaagaaa cgttggcact
       61 taaggatatt tcactagtgc tccatggacc aacaattact ggcatcattg gtccaaatgg
      121 cgctgggaaa tcaacactat taaaaggtat gctgggaatt atcccacatc aaggtcaggc
      181 atttctcgat gacaaggaag ttaaaaaatc cttacaccga attgcctatg tcgaacaaaa
      241 aatcaatatc gactacaact ttcccatcaa ggtcaaggaa tgcgtctcgt taggactatt
      301 tccctctatt cctctctttc gaagtttaaa ggctaaacat tggaagaaag tgcaagaggc
      361 ccttgaaatc gtcggcctag ctgactacgc tgaacgtcaa attagtcaac tgtctggagg
      421 tcaattccag cgggtcttga ttgccagatg tttggtgcag gaagccgact atatcctctt
      481 ggatgaaccc tttgctggga ttgactctgt cagtgaggaa atcatcatga atacgctgag
      541 agatttgaaa aaagctggga agacggttct catcgttcac cacgacctca gcaagattcc
      601 ccactacttc gatcaagtct tacttgtcaa tcgagaagtg attgcctttg gtccaacaaa
      661 agaaactttt accgaaacca atctaaaaga agcttacggt aatcaactct ttttcaatgg
      721 aggtgaccta tgattgcaga atttatcgat ggattgcaaa aattccattt cctacaaaat
      781 gccttgataa cagctattgt cgtcgggatt gtagctggag ctgtgggatg tttcattatt
      841 ctacgcggga tgtcactcat gggagatgcc atttcacatg ctgtcttacc aggtgtagcc
      901 ctctccttca tcttgggcct tgacttcttt atcggagcca ttgtctttgg attgctagct
      961 gccatcatca ttacctacat caaaggaaac tcgattatca aaagcgatac cgccatcggc
     1021 attacctttt cttctttctt agccctcggt atcatcttga ttggtgtcgc taaaagttca
     1081 actgaccttt tccatatcct ttttggtaat atcctggccg tccaagatac ggatatgttt
     1141 attactatgg gtgtgggggc agccatcctc ttgttaatct ggattttctt caagcaactc
     1201 ttgataactt cctttgatga actcttggct aaagccatgg gaatgcctgt caatttctat
     1261 cactaccttc tcatggtact cctgactctc gtgtctgtga cagccatgca aagtgtcgga
     1321 actatcctga ttgtagccat gctgattacc ccagctgcaa ctgcttatct gtatgctaat
     1381 agtctgaaaa gtatgatttt cctttcctca accttcggag ctactgcttc agttttggga
     1441 ctctttattg gctatagttt taatgttgcg gcaggttcta gtatcgtgct tacagctgct
     1501 agtttctttc tcattagctt ctttatcgct cccaaacaac gatatttgaa actgaaaaat
     1561 aaacatttgt taaaataagg ggcaaagccc taataaattg gaggatctaa tgaaaaaatt
     1621 aggtacatta ctcgttctct ttctttctgc aatcattctt gtagcatgtg ctagcggaaa
     1681 aaaagataca acttctggtc aaaaactaaa agttgttgct acaaactcaa tcatcgctga
     1741 tattactaaa aatattgctg gtgacaaaat tgaccttcat agtatcgttc cgattgggca
     1801 agacccacac gaatacgaac cacttcctga agacgttaag aaaacttctg aggctgattt
     1861 gattttctat aacggtatca accttgaaac aggtggcaat gcttggttta caaaattggt
     1921 agaaaatgcc aagaaaactg aaaacaaaga ctacttcgca gtcagcgacg gcgttgatgt
     1981 tatctacctt gaaggtcaaa atgaaaaagg aaaagaagac ccacacgctt ggcttaacct
     2041 tgaaaacggt attatttttg ctaaaaatat cgccaaacaa ttgagcgcca aagaccctaa
     2101 caataaagaa ttctatgaaa aaaatctcaa agaatatact gataagttag acaaacttga
     2161 taaagaaagt aaggataaat ttaataagat ccctgctgaa aagaaactca ttgtaaccag
     2221 cgaaggagca ttcaaatact tctctaaagc ctatggtgtc ccaagtgcct acatctggga
     2281 aatcaatact gaagaagaag gaactcctga acaaatcaag accttggttg aaaaacttcg
     2341 ccaaacaaaa gttccatcac tctttgtaga atcaagtgtg gatgaccgtc caatgaaaac
     2401 tgtttctcaa gacacaaaca tcccaatcta cgcacaaatc tttactgact ctatcgcaga
     2461 acaaggtaaa gaaggcgaca gctactacag catgatgaaa tacaaccttg acaagattgc
     2521 tgaaggattg gcaaaataag cctctgaaaa acgtcattct catgtgagct ggcgtttttt
     2581 ctatgcacac atttccggtc aaatcattgg aaaattctga ctgttttaga tacaatggaa
     2641 gaaaaaagat tggagtatcc tatggtaact tttctcggaa atcctgtgag ctttacaggt
     2701 aaacaactac aagtcggcga caaggcgctt gatttttctc ttactacaac agatctttct
     2761 aaaaaatctc tggctgattt tgatggcaag aaaaaagtct tgagtgtcgt tccttctatc
     2821 gatacaggta tctgctcaac tcaaacacgt cgttttaatg aagaattggc tggactggac
     2881 aacacggtcg tattgactgt ttcaatggac ctaccttttg ctcaaaaacg ttggtgcggt
     2941 gctgaaggcc ttgacaatgc cattatgctt tcagactact ttgaccattc tttcgggcgc
     3001 gattatgccc tcttgatcaa cgaatggcac ctattagcac gcgcagtctt tgtcctcgat
     3061 actgacaata cgattcgcta cgttgaatac gtggataata tcaattctga gccaaacttc
     3121 gaagccgcaa ttgcagctgc taaagcccta tagaaaaaat cctctgtcac aaagagctcc
     3181 ctgctctttg aaacgaagga tttttgaata ttagtaaata gaagttcaat cagctaaaaa
     3241 taactacctt actctacaga tttcagggct tctaacatat caacctttct cagataataa
     3301 ttgacgaaga aaccaagcaa ggtcaaaatg atgcttactg ttgccactgg gattacatag
     3361 acttcccagc ctacctgcgg ataaaagaga atagtcgcag gcgaaatcat ttgaatcaaa
     3421 aattggtgta aatagaaacc agctaccaga ccaagtacga ttcccacaag ggacagcaca
     3481 atcgtctcac ggtaaatgta gagggtgact tcattattat gaaaaccgag aaccttgata
     3541 gtggagagtt cacggattct ctcagctacg ttgatattgg tcagattgta aaggatgaca
     3601 atagctaata gaaccgatac gatgaccaag atggtcatgg tctgattgag tgagctagcg
     3661 atagagtcga agagtcgaat ggctgaagca ttttggacaa cgctggacac cgcagattga
     3721 ttcataagca agcccgcctg actttcgata ctagttgcac tggtatccct taatgagacc
     3781 aaataagtgt tggcttgggg tagctgtccg taaagttgct catagctagc ctgactcata
     3841 taaataaagt gaccaacgta gttctcagta atagcagcga cctttagttc cttaccttca
     3901 atttctaaag tctgcccaac cttgacacct gccagctggg cgagtttagc tgtaataacg
     3961 atgccatctt ttaatgtcag ctcctgctga tgatgttgaa gatggataaa gggagtcaaa
     4021 tcttccttct ctatcatcat aagagtaatg ttttgaagac cagccttgcc tttgaaatcc
     4081 ttgtctagcg ctttagaata gattttctgg taggctagta tctcctgccc tttcaacact
     4141 tctgctagct ctaccttgtc ctgattggtc gcactaggat tttcagagac aagcatctga
     4201 tactgttgga tttgttgaaa ctgtttagac ggaactcctg ctacagaaga ttggattccc
     4261 aaacctgcaa agagcagagc tacagaacct gccacaccaa agattgtcat caacattctc
     4321 tgcttataac gaaagatgtt gcgagctgtt accttatgag taaaactgag acgacgccag
     4381 ataaaaccga tacgctccaa taagatttta gctcctttga caggaggttt aggaagtaga
     4441 agctgggctg cttcgtcatg aagttccctc caagccacca gataggctgg taacacactc
     4501 gccaacaagc tcaagacaag agctagtaag ctataggtcc aatagaactg aatctgagtt
     4561 tctcccacca ccatgccttt tgtaatgaca cttgaaatta cattggctag caaataatga
     4621 ccaagtatac tacctagagc cgttccgaca gtcccagcta ctagtccata aaggagaaac
     4681 ttggcgataa tatccttact acgataaccc aaggccttaa aaatccctgc atgagttcgc
     4741 tcttcgtcta caaagcgagt catggtcgta aaggtcacca tggctgctac ggcataaagt
     4801 accacaggaa agatattgcc cactgctcga atactggatg aagcattgct atacataaga
     4861 tagccctgac cacctggcat ggtctgacga ttatagacct gataccttgg ctccgccaaa
     4921 tcgtccaaga cttgctgatg tttttctaat ttttcctttt cttgggctag attttgttca
     4981 gcttgcttta gtttggcctc ttccttgccc aattcctgct tggcttgggt aagttgagca
     5041 ctagcctgct ctctctgaac ttgaggaaac aaggctagtt gactttcttg agcctgtata
     5101 cgagcttgag cagctgctaa acgacgcttg ccttcctgca aattagtctg agccttgtca
     5161 agggtctctt gccccttgtc tagagactct tgtccttctt ttttcaaaag ttgcagacgt
     5221 accttgccat tatctgataa aatttgttca agctcttcct gatgttgctt ggatttttct
     5281 tcataagctg atgaaaaggc atttaaacct gctaaatctt gatatttcaa acgagctata
     5341 ttgtagactt tctgatcaaa ttgactaggt aaaatcaccc cataggctgt cagagtccca
     5401 cttccacttc ctgcgtagcc catatctcgc tgggagagga tttcagccga atccacaaaa
     5461 ccagtaatgg tataagtatg gtcttttaaa gaggaatgac cctcttcttt ttctttaaaa
     5521 ctaatctcct gtcccacgct gtattggcct tgcaaatgag tggccaaagc gatttccttg
     5581 tctgactgag gaagtcgtcc ctttcttagc tgaaaggttg aaattcgctc tggtttggag
     5641 tacagccgaa tggcatcctg cccattatcc atagtcacat ctgtcaaata gccaaactcg
     5701 acctctgcgc cctccgtctg ttttagttct tcttggtctg cttgatccaa gccatagtta
     5761 gacatgactg ccaaatccaa ggtttgagca gttgttaaat aagcattagc tgtcgtctcc
     5821 atgttgggac tggttacttt gaggcctact aaggctagag atcccaacat catcaggatc
     5881 aagatggata aaaaacgccc cttggagcct gtgaaggact gaattaagtc cttccaataa
     5941 gtttttcgct tgatcatgct agtactccaa actgtcaata tcctgaggat gctggttgag
     6001 caccacatcc ttgacactgg catcgtgcat atgaatcacg cgatcagcaa tgggcgccaa
     6061 agctccatta tgagtcacga tgatcaccgt cgctcccttt tgacgagaca tatcttggag
     6121 aattttcaaa acctgcttgc ccgtctgata atccaaggct ccagtcggtt catcacaaag
     6181 aagaatttta ggatttttgg ctaccgcgcg tgcaatggag actcgctgtt gctcccctcc
     6241 agaaagctgg gctggaaagt tattgagacg atgagccaga cctacatctg tcaaaacctg
     6301 agcaggattc aaggcatctg tcacaatttc agaagccagt tccacatttt ccttagctgt
     6361 cagattagaa actagattat aaaactgaaa aacaaacccc acatcatttc tacggtaatt
     6421 ggtgcgctgg tgggaactat aatccgcaat attaacacca tcaatccaga tttccccttc
     6481 atcattggta tccattcccc caagaaggtt aagaactgtt gacttgcctg cacctgaagc
     6541 accaaggata ataaccagct cccccttttc aatctcaaaa ttcacatcac gattggccac
     6601 aatctccgtg tccccaacct gataacgctt gtaacagtgt ttcatctcaa tataagccat
     6661 cagacccacc ctctatcaaa caaattatac tcctcgaaaa tctctcttca aaccacgtca
     6721 gcttccatct acaacctcaa aacactgttt tgagcaacct gcggctagct tcctagtttg
     6781 tgctctttga ttttcattga gtattacttc ctattatcat tataacgctt tttcaactag
     6841 ttggaaactg cctatattct taaatcctta ttaaaaggca gtttcaaatt actgccttat
     6901 aacatcttct taaaaataaa attgaacaag atcaccttct gctgttgcca ccgaaataca
     6961 atctttcccc atgcatttcg cttttatttt ttcttgattc attttaatca cactcttctc
     7021 aatatctgga taagaaactc gcaaatctcc tccactcctt gataaaatgg tcaactgtaa
     7081 gagtttttta tcttcccagc tcatgctcac ttcaaaatgt ccacgtgcca ttaagcctga
     7141 aacagaacct gttgaccaag catcaggtag ggcagctaga ggtaccagat aagctgcatg
     7201 agactggagt aacatttctg ccatgccact agtagcacca aaattaccat ctatctgaaa
     7261 aggaggatgg ctacaccaaa gatttggcaa ggtggatatc tttaactgct ctgccaataa
     7321 tttatgggct cgattgccat ctcccaaacg cgcccagaga ttgatcttat tagccttgga
     7381 ccagcctgtg ccgccatctc cacgatcatt gaggctagca cgcgccgctt caatatactc
     7441 ttgtcccttg tagctaaaga gattgccagg atagagtccc actagatggg aagcgtgccg
     7501 gtgctgggcc tccactttct cattttgaaa atactgctct tcctcctcat accactccct
     7561 gattcgacca gattgagtga tttgcaaagg atttagtaaa tcagacttct ccttaacctc
     7621 agtcaacaag ttctcatcca gtcccaattc ctgagcagcc tgaataaaat catgaaataa
     7681 ctgccaaatc agagattggt cataggtatt gccaatcgaa atcggcccat gttctggaga
     7741 ataagacgga gaagacaccc aacgctgcgc ctgctgatcc ttatgtaaaa aggcattcca
     7801 aaaacgaacc gtttccctca acatgggata aattttctcc ctaagatagt cttggtccct
     7861 ataaaatgaa taggcttcat aaacggtttg catcatccac gcattggcag ctggtgacca
     7921 accccaatag taatcccaac caggtgccgt ccaaccaaag ggagtcgctt gagtatgaac
     7981 caaccaacca ttttcctcac ctttctgaga gacgattcct gcatacttta cagccgctag
     8041 acgaccatag acacgcaaat catctacata gttgatgact ggaaagaccg tctctaggag
     8101 attggtaaca taggctggcc aataattcat ctgcagattg acatttaagt gatagtccga
     8161 attccaagga ggattgtcga ccgcattcca gactccctgt aggttagctg gtagagcatc
     8221 tgggcagtct ctggacgaac taatcaataa ataccgtcca tactggaaga acagctcctc
     8281 caaagcctgc ccttcttgtg gcttataatt ttttaacaaa tcatctgtag tggatgcgtc
     8341 aacatcagct tccaaatcca attgaacacg ctggaataag gcttggtagt cctcgatatg
     8401 ccttgatttc aattgggtat agcccttttc tttagctgtg tccaccaagt ctatcacctg
     8461 ttgctctaaa tctagtttct tgcgataatt gctagcagga ttttgggcaa aatccgtctt
     8521 agcggccaag aagagattgg cataactggc tcctgatatc tgaaccctat ctgaccaaac
     8581 tctaatatct ccatccgttt cccaagctag ataactagca aaccgcagat cattatcctt
     8641 aactcttccc ttcatcaaga tatgagaatc agtaatatcc aacttacact ccttgtaatc
     8701 agatttttcc tgctcatact ttccatcaga agccaaatca caggtcaagg atagttctat
     8761 agtaaaatct agagtttcca acccttcctt agtaaaacat tgaaccaaga gatcatctgg
     8821 aaaactcgca aaagcttcac gttcaaatcg cgttccctta tagacataag aagtcgtcgc
     8881 aagtgcctta ctaatattca actgtctctg atagtccgtc acctgagaca aagtcgtacc
     8941 ttgctggctg aactcaatgt gaatatcccc aaaagacaga taggtcccat attgactcgt
     9001 ttttggccca attaggtgct gctcagccag ttccttagcc agattgtaat ctctcttctc
     9061 caaagcctgc cgaatctcag ctaaaaaaac atactgatcc tgaagatttc caccctgata
     9121 atctgaacta tcaggaagtg gacctccaga ccagagactt ttttcattaa attgaatccg
     9181 ttcagaccct ataaggccaa atacttttgc tcctaaagaa ccattaccta tcggtaaagc
     9241 ttcttcttcc caacccatgt aagtggttga agctggttgc ttgtaggcca gtacataatc
     9301 ttgtttttta ttccttatca tacttccata ctaacataaa aagcctagaa atcaatccta
     9361 ggcttacaat tttttatttt ccaaggtagt attcagaaac aacgttcaat ttttcatcga
     9421 attcgaatac caatggtggg aagttaggga tttccacgtc catgatctca tcatctgaca
     9481 aacctttgat gtgttttaca agggcacgga ttgagttacc gtgagctcct acgaatacgt
     9541 ttttaccatc tttaagagct ggagcgattt tatcttccca gaatggaaga gcacgttcca
     9601 aagtcacttt caagttttca gcatctggga ttactgagtc gtcaagtgaa gcataacgac
     9661 ggtctgtgtg agctgagtgc tcatcatcac ggtccatatt tggaggcaat acatcgtatg
     9721 aacgacgcca gatgtgaact tgctcatcac caaattgttc agcagcttca gctttgtttt
     9781 taccagtcaa accaccgtag tgacgttcgt tcaagcgcca tgatttttca actggaaccc
     9841 ataattggtc agaagcttca agagccaagt tagttgtttt gatagcacgt ttcaatactg
     9901 aagtgtaagc ttggtcaaat tcgataccag cttctttgat caatttacca gcgtcaatcg
     9961 cttgttgtgt acctttttca gacaaatcaa catcagccca accagtgaaa aggttagctt
    10021 tgttccattc agactcaccg tggcgagcaa aaaccaattt taccattaga tggattctcc
    10081 tttaaatttt ccgaggtttc ccctcgttta tctattctat tttacacaat ttttcacaaa
    10141 aaagctagtc aaaaccaaaa aggaaaggac tgtttgacaa tgtttggcaa tcacattgtt
    10201 tttggttgtt tctattctat cttcattatt taaccttttt ttctgcgcat cgaataattt
    10261 ttgagttgac tcttttagtt tagtaaacga gtcattattt tccatagctg tgcctttgaa
    10321 accttcctca gctttagtaa aataatcatc aaaaatattt ttataaagat ctatataatc
    10381 tgcataagtg cgtattgatt caatcgcatc gtctgttaca accggtgtat cgaaagtgat
    10441 tgtttctttt gaactattgg atgttgctac tgtcttctca tcattacttg ttgaagtatt
    10501 ctttgaactt tatgttccac atgcaactag aagagaaaga gataatagag ctactcctat
    10561 accgataaac ttttttgttc ttttcatgct aaaatacctt ttattcttct gatttgaatt
    10621 tcttcaatgt tgcaaggaat agagatcctc cgataatagc aagaatcccc ccaacccatc
    10681 ctaagaatgg aatgagacta actccaccag aaacaatcat tagtacagac ggagctgcac
    10741 ctacacgctt atctccttta tagtaaacaa tagcaatgat accaagaaca agattttcaa
    10801 tcttcaaggc attcaagagt aaggcaacac ctgaaaaagc ccctgcagta gctcctccat
    10861 taagagttgt agcagctgtg tttaccgttg ttcccaacaa gacaaaagga ccaataagaa
    10921 gtaaaattcc tcccacaaga ccgacgatac cactaattaa agcaagtg
//
LOCUS       DAVKQL010000064        10895 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVKQL010000064 DAVKQL010000000
VERSION     DAVKQL010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10895
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.064"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            <1..2118
                     /locus_tag="VW561_001768"
     CDS             <1..2118
                     /locus_tag="VW561_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HEU9943995.1"
                     /translation="IKLTELYLGEQFEKTKANIEDSLSKLLTADAAIVENNNKVIDNY
                     VIEKIKNNKEALLLGLTYLERWYNFNYGETNAKDLIMYHLDFFGKSNSSALDNVIELG
                     KSGFNNLLAKNNVITYNVLLAKNYGTESLFKALEGYRKVFLPTISNNEWFKKQTKAYI
                     VEEKSTIEEGREKQGKEGTKYSIGVYDRLTNPSWKYQSMVLPLLTLPEEKTVFMIANI
                     STIGFGAYDRYRSSEYPKGEKLNKFVEDNAKEAAKRFRDHYDYWYKILDNDNKEKLYR
                     SILVYDAFKFGTDKDKDKVTHQATFETDHPAIKYFFGPAGNNVVHNGHGAYATGDAFY
                     YMAYRMLDKDGAVTYTHEMTHNSDREIYLGGYGRRSGLGPEFYAKGLLQAPDHPYDPT
                     ITINSVLKYEDSENSTRLQVADPTQRFNSAEDLHNYMHNMFDVIYMLEYLEGKAVANL
                     ETNQKYELLRKIENKFDLDQDGNNVYATNVVRRLTMDEVNKLNSFDSLIENDIITSRG
                     YKDQEYKRNGYYTIDLFSPIYSALSGEKGTPGDLMGRRIAFELLAAKGYKEGMVPYIS
                     NQYEKDAKAAGSKINSYGKEVGLVTDELVLEKVFNGQYKTWTQFKKDMYKEREKQFSK
                     LNRVNFINPNNPLSRQRNVSVTDIGVLERMIVEAVRDDAQDDVAKFYPETNSRVLKLK
                     KAIYKAYLDQTNDFRSSIFENKK"
     gene            2162..7411
                     /locus_tag="VW561_001769"
     CDS             2162..7411
                     /locus_tag="VW561_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9943996.1"
                     /translation="MSLLKKDKFSIRKIKGIVGSVFLGSLLFAPSVIGASTYHYLDYS
                     SLTQTERDQLKQGRPDESKESYALDYEKDALPNTGSSQSIMTALGLLAIGSLIVIITK
                     DNRNKKIATFLIVGATGLVTLSTASALNLNANIHESGRDGVLQISGYRYVGYLELDDK
                     TVSSVSPASTVSPVEQPKVVTEKGEPEVQPALPEAVVTDKGEPEVQPTLPEAVVTDKG
                     EPEVHEKPDYTQPIGANLVEPEVHEKLAYTEPVGTTGVDENGNLIEPPVNDIPEYTEP
                     ISTVSEVASEREELPSLHTDIRTETIPKTTIEESDPSKFIGDDSVKEVGEDGERQIVT
                     SYEELHGKKISEPVETVTILKEMKPKILVKGTKENPKEKTVPVLTLTKVTEDAMNRSA
                     NLNYELDNKDNAEISSIIAEIKDGDTVVKKVDLSKEKLTDAVQNLDLFKDYKIATTMI
                     YDRGQGSETSKLDEKTLRLELKKVEIKNISSTNLVKVNDDGTEIPSDFMSEKPSDEDV
                     KKMYLKITSRDNKVTRLAVDKIELVTEKEKELYKITASAQDLIQHVDPSKTRNEYIHY
                     IEKPVPKVNNVYYNFNELVRDMQEHPNDEFKLGADLNATNVSAFGKLYVTKDFKGKLL
                     SDGDNHYTIHNLSRPLFGNVIGGTIKNINLGNVDINMPWANQVAAVANIIKGGTTIEN
                     VKVKGNIVGKDWVSGFIDKIDNQGTLRNVAFIGNVTSVGDGGQFLTGIVGENWKGLVE
                     RAYVNANLIGKKAKAAGIAYWTQNEGNNNTVRQEGAIKKSIAKGTIQVTEAIESGGVV
                     GSMKHHGSVEDSVSMMKVPNGEIFYGSSDIDYDDGYWTGDNVRRNYVVIGVSDGHSSY
                     QRSKDKNRIRPISEEEAKSKIEATGITADKYEINEPVVNRLNRLTRREDEYKSTQDYK
                     VDRDLAYRNIEKLQPFYNKEWIVNQGNKLAEDSNLAKKEVLSVTGMKDGQFVTDLSDI
                     DHVMIHYADKTKEIKAVHQKESKVAQVREYSIDGLDDIVYTPNMVDKNRDQLIKDIKD
                     RLATVELISPEVRALMDKRDTSRDPNANSDERKNGYIRDLYFEESFSETKANLDKLVK
                     SLIENADHQLNSDEAAMKALVKKVDENKAKIVMALTYLNRYYDIKYGDMTIKNLMMFK
                     PDFYGKSVDLLDFLIRIGSSERNIKGDRTLDAYRDMIGGTIGKSELHGFLDYNMRLFT
                     NDTDLNDWFIHAAKNVYIVEPKTTNPDFVNKRHRAFDGLNNGVHNRMILPLLTLKNAH
                     MFLISTYNTMAYSSFEKYGKYTEAEREAFKDKIKEVAHAQQTYLDFWSRLALPSVRDQ
                     LLKSQNRVPTPVWDNQNYHNVEGVNRMGYDKNNKPIAPIRELYGPTWRYHTTNWYMGA
                     MASIFQDPNNNDQVYFMGTNMISPFGISAFTHETTHVNDRMLYFGGHRHRQGTDVEAY
                     AQGMLQTPDKSGNGEYGALGLNMAYHRENDGDQWYNYDPDKLKTREDIDRYMRNYNDA
                     LMMLDHLEADAVIPKLHGNISRWFKKMDRQYRKNGELHQFDKVRELTEDEKKKIVINN
                     IDDLVNNNLMTKHGAPSDRTYNPEDFDSAYVNINMMTGIYGGNTSQGAPGAASFKHNT
                     FRMWGYFGYENGFISYASSKYQGEADKTNKKLLGDDFIIKKVSKDKFNNLEEWKKQYF
                     KDVKSKAEKGFTAIEIDGRQITNYAQLKTLFAEAVQKDIDGMSDPKIKDHFKNTVDLK
                     SKVFKALLKNTDGFFNKLFKEDI"
     gene            7657..8508
                     /gene="ylqF"
                     /locus_tag="VW561_001770"
     CDS             7657..8508
                     /gene="ylqF"
                     /locus_tag="VW561_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEU9943997.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWCQYFESQEIQTLAVNSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            8495..9274
                     /locus_tag="VW561_001771"
     CDS             8495..9274
                     /locus_tag="VW561_001771"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU9943998.1"
                     /translation="MATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            9290..10840
                     /locus_tag="VW561_001772"
     CDS             9290..10840
                     /locus_tag="VW561_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU9943999.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHMIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALIGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
BASE COUNT         3790 a         1723 c         2198 g         3184 t
ORIGIN      
        1 ataaaattaa cagagttata tttaggtgaa caatttgaaa aaacaaaagc aaatatagaa
       61 gacagtttat caaaactact tactgcagat gctgcaatag tggaaaataa caataaggta
      121 atagataatt atgtaattga gaaaataaaa aataataagg aagccttatt gttaggatta
      181 acgtatttag aacgctggta taattttaat tatggagaaa ccaatgctaa agatttaata
      241 atgtaccact tggatttctt tggtaaatca aatagttctg cattagataa tgttattgag
      301 ttaggtaaat ctggatttaa caatttatta gcaaaaaata atgtaattac ttataatgta
      361 ttattagcga aaaattatgg aactgaaagt ttatttaaag cattagaagg atatagaaaa
      421 gtattcttac caacaatttc taacaatgag tggtttaaaa aacaaacaaa agcatatatt
      481 gtagaagaaa aatctacaat tgaagaggga agagaaaaac aaggaaaaga gggaactaaa
      541 tattctattg gggtatatga tagattaact aatccatctt ggaaatatca aagtatggtc
      601 ttaccactat taactttacc tgaagaaaaa acagtgttta tgatagcgaa tatttctact
      661 attggatttg gagcctatga cagatataga agttcagaat atcctaaggg agaaaagtta
      721 aacaaatttg tagaagataa cgccaaagaa gcggctaaac gatttagaga tcattatgat
      781 tattggtata agatattaga taacgataat aaagaaaaat tatatagaag tattctagta
      841 tatgatgctt tcaaatttgg tactgacaaa gataaagaca aagtaactca tcaagctact
      901 tttgaaactg atcatccagc tataaaatac ttctttggac ctgcgggaaa taatgttgtt
      961 cataatggac acggagcata tgctaccgga gatgcattct attatatggc atatcgtatg
     1021 ttggataaag atggagctgt aacatatact catgaaatga cacataactc agatagagag
     1081 atttatcttg gaggatatgg aagaagaagc ggattaggac ctgagtttta tgcaaaagga
     1141 ttattacaag ctccggatca tccatatgat ccaacaataa caattaactc tgtcttgaaa
     1201 tatgaggatt cagaaaattc aacaagactt caagtagctg atccaactca acgatttaat
     1261 agtgcagaag acctacataa ttatatgcat aatatgtttg atgtaattta tatgttagaa
     1321 tatttagaag gaaaagctgt agctaattta gaaacaaatc aaaaatatga gttactaaga
     1381 aaaattgaaa ataaatttga tcttgatcaa gacggtaata atgtatacgc gacaaatgtt
     1441 gttcgcagat taacgatgga tgaagttaat aaattaaatt cttttgatag tttaattgaa
     1501 aatgatatta ttactagcag agggtacaaa gaccaagaat ataagagaaa tggttattat
     1561 acaattgatt tattttcacc gatttattca gctcttagtg gagagaaagg gactcctggt
     1621 gatttaatgg gacgccgtat agcattcgaa ttattagctg caaaaggata taaagagggc
     1681 atggtacctt atatctcaaa tcaatatgaa aaagatgcaa aagcggcggg aagtaagatt
     1741 aattcttatg gtaaagaagt aggtttagtt acagatgaat tagtacttga aaaagtattt
     1801 aatggtcaat ataaaacttg gacacaattc aagaaggata tgtataagga aagagaaaaa
     1861 caattcagta aactaaatag agttaacttt attaatccta ataatccatt gagtcgtcaa
     1921 cgaaatgtat cggttacgga tataggtgta ctagagagaa tgattgtcga agcagtgaga
     1981 gatgatgcgc aagatgacgt agcaaaattt tacccagaaa caaatagtag agttcttaaa
     2041 ctgaaaaaag caatatataa agcttatctt gaccaaacaa atgattttag aagttcaatt
     2101 tttgagaata aaaaatagtg tttactatta ggaaataaaa agtgaatgtg gaggattaga
     2161 attgtcatta ttaaaaaaag ataaattttc cattcggaaa ataaagggta ttgttggttc
     2221 ggtattcttg gggagtcttt tatttgcacc gtcagttatt ggtgcatcga cttatcatta
     2281 cttagattat agtagtttga cacaaactga acgtgatcaa ctcaaacaag gtagacctga
     2341 tgaatccaaa gagtcatatg ctttggatta tgaaaaagac gcactaccaa acacaggcag
     2401 ttctcaatct attatgactg cattgggttt gttagctatc gggagtctta ttgtgattat
     2461 cacaaaagat aatagaaata aaaaaatagc tacatttttg attgtagggg cgacaggttt
     2521 agttacacta tcaactgctt cagcacttaa tttgaatgct aacatccatg agtctggacg
     2581 tgatggtgtg ttgcaaattt ctggttacag atatgttggt tacttggagc ttgatgataa
     2641 aactgtttcg tcggtttctc cagcatcaac tgtttcgcca gttgagcaac ccaaagttgt
     2701 aacagagaaa ggggaaccgg aggttcaacc agccttaccc gaagctgttg tgactgacaa
     2761 aggcgaaccg gaagtacaac cgacattacc agaagctgtt gtgactgaca aaggcgaacc
     2821 agaagttcat gagaagcctg actatacgca accaataggt gcaaaccttg ttgagcctga
     2881 agttcatgag aaactagcat acactgaacc agtaggtaca acaggagtgg atgaaaatgg
     2941 aaacttgatt gaaccacctg ttaacgacat tccagagtat actgaaccaa tatctacagt
     3001 ttcagaagtt gcatcagaaa gagaagagtt gccttcttta cataccgata ttcgtacaga
     3061 gactattcct aaaaccacta tagaagaatc tgatcctagc aaatttatag gcgatgattc
     3121 tgttaaagaa gttggtgagg atggcgaacg tcaaattgtt actagctatg aagagttaca
     3181 tggcaaaaaa attagtgaac cagttgaaac ggtaacaatt ttgaaagaaa tgaaacctaa
     3241 aattcttgta aaaggtacaa aagaaaatcc aaaagaaaaa acagttcccg ttttgacctt
     3301 gaccaaagta acagaagatg ctatgaatcg ttctgccaac ttaaattatg agcttgataa
     3361 taaagataat gctgaaatat caagtattat agctgaaatt aaagatggtg atacagtagt
     3421 taaaaaggtt gatttatcta aagaaaaatt aactgacgct gtacaaaatt tggatttgtt
     3481 taaagattat aagattgcaa caacaatgat ttatgatcgt ggtcaaggtt ctgaaacgtc
     3541 taaactggat gaaaaaactt tgcgtttgga gttgaaaaag gtcgaaatta aaaatatttc
     3601 atctactaat ttagtgaagg taaatgatga tggtactgaa atacctagtg atttcatgag
     3661 cgaaaaacct tctgatgaag atgtgaagaa aatgtactta aaaatcacta gtcgtgataa
     3721 taaagtaact cgtttagcag ttgataagat tgagttagta actgaaaaag aaaaagaact
     3781 ttataaaatt acagcaagcg ctcaagattt aattcaacat gtcgatccat ctaagactag
     3841 aaatgaatac attcattaca ttgaaaaacc agttccaaaa gtcaataatg tatattacaa
     3901 tttcaatgaa cttgtgagag atatgcaaga acatccaaat gacgagttta aattgggtgc
     3961 tgaccttaat gccacaaatg tatcagcatt tggtaaatta tatgttacga aagatttcaa
     4021 aggtaagctc ttgagtgatg gtgataatca ctataccatt cataacttgt cacgtccgtt
     4081 atttggcaat gttattggtg gaacgattaa gaatattaat cttggtaatg ttgacataaa
     4141 tatgccatgg gcgaaccaag ttgcagctgt agcgaatatt attaaaggtg gaactaccat
     4201 tgaaaatgtt aaggttaaag gtaatatagt aggtaaagat tgggtatctg gatttattga
     4261 taagattgat aaccaaggta cactcagaaa tgtggccttt atcggtaacg taacttctgt
     4321 cggcgatggt ggacaattct tgactggtat cgttggtgag aactggaaag gactcgtcga
     4381 acgcgcgtat gttaacgcaa atcttattgg caagaaagcc aaggctgcgg gcattgctta
     4441 ttggacacaa aatgaaggaa ataacaatac tgttcgtcag gaaggagcaa tcaaaaaatc
     4501 tattgctaag ggaacaattc aagtaactga ggcgattgaa agtggtggtg ttgtaggaag
     4561 catgaagcac cacggttctg tcgaagattc agtttccatg atgaaggtac ctaatggtga
     4621 aattttctat ggttctagtg atattgatta tgatgatggc tactggacag gagataatgt
     4681 aagacgaaat tatgttgtaa taggtgttag tgatgggcat tctagctacc agcgttcaaa
     4741 agataaaaat cgtatcagac ctatttctga ggaagaggca aaatctaaaa ttgaagctac
     4801 aggaattacc gcagataaat acgaaatcaa tgaacctgtg gtcaatcgct taaatcgttt
     4861 aaccagaaga gaagatgaat ataagtcaac ccaagactat aaagtagatc gagatctggc
     4921 ttatcgtaat attgagaaac tgcaaccatt ttataacaaa gaatggattg tcaatcaagg
     4981 taacaaactt gcagaagatt caaacttagc taagaaagaa gtcctttctg ttacgggtat
     5041 gaaggatggt caattcgtga ccgatttatc agatattgat catgtgatga ttcactatgc
     5101 agataagact aaagaaatca aagctgttca tcaaaaagag tctaaagtag cgcaagttcg
     5161 tgagtatagt atcgatggat tagatgacat tgtttataca ccaaacatgg ttgataaaaa
     5221 tcgtgatcaa ttgattaaag acattaaaga taggttagca actgttgaat taatttctcc
     5281 cgaagtccgt gctttgatgg ataaacgaga tacatctaga gatcctaatg ctaattcgga
     5341 tgagcgtaag aatggttata tccgagacct ttatttcgaa gaaagttttt ctgaaacgaa
     5401 agctaatctt gataaattgg tcaaatcttt gattgaaaat gctgaccatc aattaaatag
     5461 tgacgaagca gctatgaaag cacttgttaa gaaggtggat gaaaataaag ctaaaatcgt
     5521 gatggctctt acttacctta atcgttacta tgatataaag tatggggata tgactatcaa
     5581 aaacttgatg atgtttaaac ctgatttcta tggtaagtca gttgaccttc ttgacttctt
     5641 gattcgaatt ggatcaagtg agcgaaatat caagggtgac agaactttgg atgcttatcg
     5701 tgatatgatt ggtggaacta ttggtaagtc tgaattacat ggcttcttag attacaacat
     5761 gcgtctcttc actaacgata ctgatttaaa tgattggttt atccatgcgg ctaagaacgt
     5821 atacattgtg gagcctaaaa caacgaaccc agattttgtt aataaacgac atcgtgcatt
     5881 tgatggtttg aataatggtg ttcataatcg tatgatccta cctctattaa ctcttaagaa
     5941 tgcacatatg ttcttgattt cgacttacaa tacaatggct tatagctctt ttgaaaaata
     6001 tggtaagtat acagaagctg aacgagaagc atttaaagat aaaattaaag aagttgctca
     6061 cgcacaacaa acttatcttg acttctggtc gcgtttagca ttgccaagcg tacgtgacca
     6121 attactcaag agtcaaaata gggttccaac acctgtttgg gataaccaaa actatcataa
     6181 cgtcgaaggt gtaaatcgta tgggttatga taagaataat aaaccaattg cacctattcg
     6241 tgaattatat ggacctactt ggcgttatca tactacaaat tggtacatgg gagctatggc
     6301 atctattttc caagatccca ataataatga ccaagtttat ttcatgggaa caaatatgat
     6361 tagtccgttt ggtatttcag cctttactca cgaaacaaca cacgtaaacg atagaatgct
     6421 ttacttcggt gggcatagac accgtcaagg tacagacgtt gaagcctatg cgcaaggtat
     6481 gttgcagaca cctgataaat ctggtaacgg tgaatatggt gctcttggtc ttaacatggc
     6541 ttatcatcgt gaaaatgacg gtgaccaatg gtataattat gatccagata aattgaaaac
     6601 tcgtgaagat atcgaccgtt atatgagaaa ttataatgat gctcttatga tgttagacca
     6661 tcttgaggct gatgctgtta tccctaaatt acatggtaat attagtcgat ggttcaagaa
     6721 aatggataga caatatcgca aaaatggaga gctacatcaa tttgataaag ttcgtgaatt
     6781 aactgaagat gagaagaaga aaattgttat caacaacatt gatgatttag tcaacaataa
     6841 tttgatgaca aaacatggcg ctcctagtga cagaacatat aatccagaag attttgatag
     6901 tgcttacgta aatattaata tgatgaccgg tatctatggt ggtaatacta gccaaggagc
     6961 acctggagct gcatcattca agcataatac tttccgtatg tggggctatt ttggttatga
     7021 aaatggattc attagttatg catctagcaa atatcaaggc gaagctgaca aaaccaataa
     7081 aaaattattg ggtgatgatt tcattatcaa gaaagtatct aaggataaat tcaataatct
     7141 tgaagagtgg aagaaacaat acttcaaaga tgttaagtca aaagcagaaa aaggatttac
     7201 tgctattgaa attgatggtc gtcaaattac aaattatgct cagttgaaaa ctttatttgc
     7261 tgaagctgtt caaaaagata ttgatggtat gtcagatccg aaaattaaag atcatttcaa
     7321 aaatacagta gacttgaaat ccaaagtatt caaagccctt cttaaaaaca cagacggttt
     7381 ctttaataaa ctgtttaaag aagacattta aatgtttaaa atataaagag gacagttttt
     7441 tgctccctct gaaaagtcat catttgatgg cttttttcta tatagggtaa aagatagggt
     7501 aaaaggctat catcggacaa aataaagaag gcatgatata atataaagta gatttttata
     7561 tcataaaaca agaactgttt ggacatcatt catttttaga ttgagatctc ttagttctag
     7621 attaacagct tcagagctac gaaaggaaaa aactcaatgg ctactattca atggtttcct
     7681 ggtcacatgt ctaaagcgcg tcgacaggtg caggaaaatt taaaatttgt tgattttgtg
     7741 acgattttag tagatgcacg cttgcctcta tctagtcaaa atcctatgtt gaccaagatt
     7801 gttggtgata aaccaaaact cttgatttta aacaaggccg acttggctga tccagcaatg
     7861 accaaggaat ggtgtcagta ttttgaatca caagaaatcc agacgctagc tgtcaactcc
     7921 aaagagcaag tgactgtaaa agttgtaaca gatgcggcca agaagctcat ggctgataag
     7981 attgctcgcc agaaagaacg tgggattcag attgaaacct tgcgtaccat gattatcggg
     8041 attccaaacg ctggtaaatc cactctgatg aaccgtttgg ctggtaaaaa gattgctgtt
     8101 gttggaaaca agccaggggt cacaaaaggt caacaatggc ttaaaaccaa taaagatctg
     8161 gaaatcttgg atacaccggg gattctctgg cctaagtttg aggatgaaac tgttgcactt
     8221 aagttggcat tgactggagc tatcaaggat cagttgcttc ctatggatga ggttaccatt
     8281 tttggtatca attatttcaa agaacattat ccagaaaagc tggctgaacg cttcaaacaa
     8341 atgaaaattg aagaagaagc gcctgtgatt attatggata tgacccgcgc cctcggtttc
     8401 cgtgatgact atgaccgttt ttacagtctc ttcgtgaagg aagtccgtga tggcaaactc
     8461 ggtaactata ccttagatac attggaagac ctcgatggca acgattaaag aaatcaaaga
     8521 actccttgtg acagtcaagg agttagaaag ccctattttt ttagagcttg aaaaggataa
     8581 tcgctcagga gttcaaaagg aaatcagcaa gcgtaaaaga gccattcaag ctgaattaga
     8641 tgaaaatttg cgcttggaat ccatgctttc ttatgaaaaa gaactttata agcaaggatt
     8701 gaccttaatt gcaggtattg atgaggttgg tcgtggtcct cttgctggtc ctgtagtcgc
     8761 tgcggccgtt attttaccta aaaattgtaa gattaaaggt ctcaacgaca gcaagaaaat
     8821 tcctaaaaag aaacatctgg agattttcca agccgttcaa gaccaagcct tgtcgattgg
     8881 aattggtatc atagataatc aggtcatcga ccaagtcaac atctatgaag caaccaaact
     8941 agccatgcaa gaagcaatct cccagctcag ccctcaacca gagcaccttt tgattgatgc
     9001 catgaaactg gacttgccca tttcacaaac ctccattatc aaaggagatg ccaactccct
     9061 ctctatcgca gcagcatcta tagtagccaa ggtaacacgt gatgaattga tgaaagaata
     9121 cgatcagcag ttccctggct atgatttcgc tactaatgca ggatatggca cagctaaaca
     9181 tctggaaggc ctcacaaaac taggagttac cccaattcac cgaaccagct ttgaacccgt
     9241 taaatcactg gttttaggta aaaaagaaag ttaattgaaa ggaaatacca tggaggaaca
     9301 gtcggaaata gtccgttcta agaaagaatt cgcctttgca tccagcacta tactatccca
     9361 agttggtcga ggaatcattg tcggcctcat cgttggaatt atcgtcggat cctttcgttt
     9421 cttaattgaa aagggcttcc acatgataca aggagtttat caagatcaag ggtacttagt
     9481 gcgcaatctt tttgtactgg ttttgtttta tatactcatc tgttggctca gtgccaaact
     9541 aacacggtca gaaaaagata ttaaaggctc aggaattcct caagtcgaag ccgaactgaa
     9601 aggcctcatg tccctcaact ggtggggcat tctttggaaa aaatatgtgc taggtattct
     9661 cgctattgcc agtggactca tgctgggtcg agagggaccc agtattcaac ttggagcagt
     9721 tggtggtaaa ggaattgcca agtggctcaa atccagtcca gtagaggaac gttccttgat
     9781 tgccagtgga gctgcagcag gtttagccgc agcctttaat gctcctattg cagcacttct
     9841 ctttgttgta gaagaagtct atcaccattt ttcgcgcttt ttctgggtct caactctagc
     9901 agccagcatc gtagcaaact ttgtgtctct actcatgttc ggtttgacac cagtattgga
     9961 tatgccagat aacattcctc ccatgaccct agatcagtat tggatatatc tcgtcatggg
    10021 aattttcctt ggattttcag gctttctcta tgagaaagct gtattaaacg ttggaagagt
    10081 ttatgacttg attggtcaaa aaatccattt ggatagagct tattatccca tcttggcttt
    10141 tatccttatc ataccagtcg gaatcttctt acctcaaatc attggtggcg gaaatcagct
    10201 tgtcctttct ttaactgaac aaaattttag tttccaagtt ttattagctt actttttgat
    10261 ccgctttatt tggagtatga ttagctatgg aagtggactg ccaggaggaa ttttcctccc
    10321 cattttagct cttggttctt tgcttggtgc cttaattggt gttatctgtg tcaatcttgg
    10381 acttgtcagt caagagcaat tccctatatt tgtcattcta ggaatgagtg gctattttgg
    10441 agccatatca aaagctccct taaccgctat gatcctcgta actgagatgg taggagatat
    10501 tcgcaacctc atgccacttg gtcttgtcac tcttgtttct tatattatca tggatttgct
    10561 caaaggtacg ccagtctatg aagccatgct ggaaaaaatg cttccagaag aagtatctag
    10621 cgaaggagaa gttacactta tcgaaatacc agtttctgat aaaattgctg ggaaacaagt
    10681 tcatgaactc aacttaccac acaacgtcct catcacaact caagtccata atggcaagag
    10741 ccaaacagtt aacggctcaa ccagaatgta tctgggtgat atgattcacc tggttattcc
    10801 aaaaagtgaa attggaaaag tcaaagattt gttgttgtag tacaatcatt tacataattt
    10861 atattatgta aatgattggc ttgatttatt tagaa
//
LOCUS       DAVKQL010000065        10561 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVKQL010000065 DAVKQL010000000
VERSION     DAVKQL010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10561
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.065"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(87..1511)
                     /locus_tag="VW561_001773"
     CDS             complement(87..1511)
                     /locus_tag="VW561_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU9944000.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAIERAAYLHKTAAILERDKEKIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2056..4335)
                     /gene="pulA"
                     /locus_tag="VW561_001774"
     CDS             complement(2056..4335)
                     /gene="pulA"
                     /locus_tag="VW561_001774"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEU9944001.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGTYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4447..6405)
                     /gene="ligA"
                     /locus_tag="VW561_001775"
     CDS             complement(4447..6405)
                     /gene="ligA"
                     /locus_tag="VW561_001775"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU9944002.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQARQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLYQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6498..7676)
                     /locus_tag="VW561_001776"
     CDS             complement(6498..7676)
                     /locus_tag="VW561_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9944003.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLPFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7756..8619)
                     /locus_tag="VW561_001777"
     CDS             complement(7756..8619)
                     /locus_tag="VW561_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU9944004.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTPLYFELNWILLQGLICQRDATYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKDIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAIHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8622..10523)
                     /locus_tag="VW561_001778"
     CDS             complement(8622..10523)
                     /locus_tag="VW561_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9944005.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNNLRRTERQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETIAWLENYLVNYSGALLIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLVTEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYIEKRATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSEQV"
BASE COUNT         3163 a         2340 c         1899 g         3159 t
ORIGIN      
        1 accaaaaagt tcgtattttt tctataatta ttattatttt atagcaaaaa aataaaacca
       61 ggaaaacaat ttcctgattt tacacgttat ttcacatcaa aaacaatgga tttgacattt
      121 gtcatcgctt caatgctata tttaatccct tgcactccag ctccagaacc tttgacacca
      181 aggaacggga aattatctgg accacgctgg gttttattat taatgtggac tgtacctact
      241 tcaagttttt cagcaatttc aaaggctttt ttgaaatcat ttgtaaagac tgatgattga
      301 aggccgaatt cagattcgtt ggcaaaggca atagcttcct ctacactagc cacacgaatg
      361 attggtaaaa caggaccaaa tggctcttcc catgccactt tcatatcttt tgtaacttgg
      421 tcaaaaagca ctggccagag aagattgccc tcacgtttga ttggtgtaag agcctgagcc
      481 tctttttctt gtgcatcctc aatcaagccc caaatgaagt cggctgaagc attgtcaata
      541 acaggtgtaa tatcagcatt gtcaaatgga tcaccaactg ttaatttaga aacttcttcc
      601 tgaagcaaag tagctaattt atctgctaca ctttcgagaa caatgacacg tttaatggcc
      661 gtgcaacgtt gtcctgagta gctaaaggct cctgcaacaa tttgcttggc agcatgttcc
      721 aaatctgcat cttctagtac aagagctgca tctttcccac caagttccaa catgatagga
      781 cgcataccag ctaaacgacc aatacgttct ccaatagggg ttgaacctgt aaagttgatg
      841 aagttgactt ctttgtgctc aatgatataa tccccaattt ctgaaccacg acctgtaatg
      901 gtgttgaaaa cacctgccgg aatccctgct tcttcaaatg ctttagccaa caagagtcca
      961 gaaatggaac cttgtgttgg tggcttaaac atgaccacat tccctgcaat caaggcaggt
     1021 gcaattttag aagcagataa attaactgga taattaaagg gagcaatcgc tagcacgata
     1081 ccaactggtt cacgacggac aacagccagt ttgtttttac ttgctgcctc aaaaccacca
     1141 ccttccattg cttgtccagt gatacggaga ccttcctcag cagcataacg aatcaagtct
     1201 gctgtacgca ctacttctcc aattgctgct ttaatccctt ttgctacttc tttggcaagg
     1261 atagtaccaa ttttttcctt atcgcgttct aaaatagctg ctgttttatg caaataagcc
     1321 gcacgttcaa ttgctgataa agctcgccat gctggcaggg ctgcacgcgc agcttgcata
     1381 gcctcatcag cttcagtctg agtcatggct ggaactgtac ccaattcttc ttgattgatt
     1441 ggtgaataaa tcgtaatttc ttgttcagat gatttccatt ttccatttac taaattctga
     1501 tatcttgtca aattcttgtc ctcctgaaaa tgattaagat atattttacc aaattttcag
     1561 aaaatcacaa ccctttttgc aaaatttttg agattatttt cacaagcttg atttttcaaa
     1621 gtatactcaa taaaaataaa aaaaatccac tacatcaagg cgagactaat gtggtttgaa
     1681 gaaattttcg aagagcgtga atgagtatca tctatagtaa aataaaaaaa ctgaacaatt
     1741 tggttgggga cagccaaacc aatttctcac aatgtttcag aaacaagggt gtgctattcc
     1801 aatttcagcc tactataact gtcatagatt gctgaaacaa agtctaggta aaagtcttca
     1861 taataaaaag acctcctatc aaatgttcaa aaactttgat aggaggtctt gtttcgtgaa
     1921 aacatttatc aaattttcta tacaagtgag ctgttagcca ggttctttct attctttcaa
     1981 tttcaatgaa tggatttttt accaatactc ataactggga atttttctgt ctaaaaatag
     2041 cgagatagat ggtatttata aaacactcaa gacagctaga ctaatatcat ttaaaacatt
     2101 atcttctttt gagcgactgt tggttaccaa catagctaaa tttcctgcat tttcaaattg
     2161 atagggttct gatttagcat tcacaaccac caagaggtgt tctttgccgt gaacttcata
     2221 gataaggcag ccgctatgtt caatcgcaga atgcacaaag acatgatggt aaatttcatc
     2281 atagctagag taagaaaagg caccagtttt tgtcttcaat cggatgactt gacggataaa
     2341 ctcaatactg tcttgacgct cattaatcaa gttccagttc acttggttca cactgtcagg
     2401 agcattatag ctattcatcg cacgctctct atcatcatgg gtcaactcac cattttcacc
     2461 agtcgcaacc agtttggtac gaccaaattc ttgaccgatt tccataaagg ccatcccctg
     2521 catgagcaga ttcatggctg tggcagtttc gaccttgcgc atgatttgct ctgaactttg
     2581 gtctggatga agggttgcca ataaatcgtg aagattgtaa ttgtcatggg cttctacata
     2641 gttaagcacc tgatttggat gtgtataggt tcctaattca cgacttccta ggattgcttt
     2701 agctagaatt ggctctgtcg cagcaccact gacaaaacct gacttgatag caccataaac
     2761 ttctccccct ttgacagcat cgcgctgatt gtcattaaag aaaccaatat ttggcatctg
     2821 gtaggcattg tccttcttgg ccttatcata aggggcaaga cctgttccca tatcccatcc
     2881 ttctccatag aggataatgt tggagtcgat ttcatccaag ctttgacgaa tcatctgcat
     2941 ggtcttgaca tcatgaatcc ccatcaagtc aaaacggaag ccgtcaatat tatattcctg
     3001 cacccagtat agaagagaat caatcatata cttgcgaaac atttcgtgtt cactggctgt
     3061 ttcatttcca acacccgttc cattctggaa ggtaccatct ggattcatac gataatagta
     3121 atcagggact gttgtttgga atggtgcatc aacaactgag aaggtatggt tatagactac
     3181 atccataatg actccaatac ccgcatcgtg ataagcttga accatcacct tcaaatcacg
     3241 aatgacctga gctggatcat ctggattagt tgaaaaacta gtttctggcg cgttatagtt
     3301 ttgtggatca taaccccagt tgtaggttac atttccatcc tcatcgtatt ctttatgacg
     3361 gtctgcaatt ggttgcaatt gaacataatt gtagcccagc ttcttgatgt aatcaaaagc
     3421 agttgactgg ccgtattggt taactgttcc agcctgagca gcacccaaga aagttcctcg
     3481 aagatgttca tctacacccg atgtaggtga tttagtcaaa tcacgaatgt gcatttcaca
     3541 gataactgcc ttacatggat tttccaagcg ccaagtagcc tccgaaccgt gcttaacctc
     3601 gaagttttca acttgctttt ctacatggct cagaatagct gaacgtttgc catcagggct
     3661 ggtcgcgatt gtataaggat cacgtgtcag tgtttggtga tgagggaatt ggacttgata
     3721 ctgataagtc ttacctacca aatcttcttc aacatccaaa ctccagacac cgattgtatt
     3781 gtccttatga ttataagagt agctattgcc tcttttcatc tcaaaagtct tccaaacggg
     3841 tgcatcatta gcagctgatt cataaacgac aacttgtact tctgtcgctg taggtgacca
     3901 gagggaaaaa tgagcctgat tgtcctctac acggcaaccc aattctcctt ggtaacccca
     3961 atgatgatca aaactagcac tgttaatggc cttatcaaag gcaaaaggat tttgattttt
     4021 atagaaaggg ctggcaatag caggattttc agagtaataa atcctatcat cgccttccaa
     4081 aatccagacc tctgttaata ggggatagtg attaaaacgg atagaatatt ctttactagt
     4141 ttgacctgta tgaaccacaa aattcaagct ttctataaca tgtgaacttg ggtgttcaaa
     4201 gctaaataaa gctccaaaat aatcttcttt gtaggttagc aaatcaattc gttgatcctg
     4261 actttttaca aaggagcaag tgtcatattc tccattctta cgatggtaat gaatgcgcat
     4321 agggtagtta tacatttttt atttttcctt tttactttgt ttctatttca ctaataaatt
     4381 tttgtcaatc tcgtctcaat taacagacat agtcatattc tctaaactct gtttttaaac
     4441 gatccattac aaactttcta gccatgcctc atctctgacc tggataccaa gttcttgtgc
     4501 tttttgcagt ttacttccag cgtctgcacc taccacgacg aggtcggtct ttttagaaac
     4561 actacctgtc actttggcac ccagactttc gagtttactt ttagcttctg agcgcttgag
     4621 tcgttccaat tttcctgtca ataccacggt caaacctgac aaggccgcat ccgctactac
     4681 cgtctgtcct ttatagtcca gattgacccc agtttctttc aattctctga gcagaatttc
     4741 agagccttct gtcgcaaaat aagtctgaag acttttggca atcacgccac ctagactttc
     4801 aatactggcc acttcctctg aatctgcctg atacagattt tcaattgaat gaaaatattg
     4861 aagtaaaagc tgactagcct tgcttccgac atgacgaatt cccaaaccaa ataagagctt
     4921 ctcggcagaa ttttcctttg atgcttggat agcctgatac agtttagcag cggacttttc
     4981 cttaaccccc tctaaaagga ggaaatcctc ttcttgcaaa cgataaatat ccgccacatc
     5041 cttgactaaa ttagcagcaa aaagcttctc aacaatagat ggaccaaggc ctgtaatatt
     5101 catagcatca cgagaagcaa agtgaatcaa gccttccatg atttgagcag ggcaacgcgg
     5161 attgatacaa cgtagggcca cttcatcttc aaagtgcaac aagtcagagt tacaacttgg
     5221 acagtttgta gggatatcta gtttttcttc agaaacccgt ttggactcta ccacacgtaa
     5281 aacggcaggg atgatgtcac cagccttata tacaatgacc gtatcgtctt ttcggatatc
     5341 tttttcagca atataatcta cattgtgcag ggtcgcacgg ctaacagtcg taccggcaag
     5401 ttgtactggt gttagattag cagttggagt tacaacaccg gtacggccaa ctgtccagtc
     5461 aactgataag agttgagctt ctttttcttc ggcagggaac ttgtaggcta ctgcccactt
     5521 tggagcctta actgtaaaac caagttcttc ttgacttgct aggtcgttga ccttgattac
     5581 cactccatca atatcgtaag gcagattttc ccgttcctgt cctacttctt ggataaaatt
     5641 ccagatttca tctatgtttt cagccaagat tcgcttagga ttgaccacaa aacctagttg
     5701 ttctaggtac ttcaaaccct tttcttggct atcacgagtt gaagggctgg cttcttgata
     5761 gagaaacgtt gcaagattac gcttggcaac tactgctgta tccaactgac gcagagttcc
     5821 tgctgccgca ttacgaggat tagcaaattc aggctctcca ttttcttggc gcgcttggtt
     5881 aacttggtca aaggaagcgc gtggcatgta acattcccca cgaactgtga tatctagttc
     5941 ttctggcaaa gtcaaaggga tgtccttaac acgcttgagg ttttctgtga tattttcacc
     6001 aattgaacca tctccacgtg ttaccccagc aaccaaaatc cccttttcat aagtcagcga
     6061 gatagataag ccatcgattt tcagctcaca aatataggtc ggatgagcca cttccttacg
     6121 aacacgcgca tcaaaagcat ctagctcctc acgtgaaaaa gcatcctgca aactataaag
     6181 aggatactga tgactgtatt tttcaaaacc atctaaaacc ttgccaccaa cacgatgagt
     6241 cggactgtct gctagcactt gctctggata agcagtttct aactcgacca actcacggta
     6301 aaggcggtca tactcactgt ctgaaaccga gggattatcg ctggtatagt actcagtcgc
     6361 atagcgattg agcaaagcga ctaactcatt cattctttta ttcataagac cattttacca
     6421 taaaacaagc cctcctcaca aacgagaagg gcggaaaaaa cacttagttt gaaattattt
     6481 ttgaaactca agcaacctta tatcaatttt tcaaaatgag ttcgaacata aataaacgat
     6541 atacaagaca agatgataac accacttcca attatcagga aagaagagag atgtacactt
     6601 ggcaagactg tcataaatcc ttttgcaata ggcataaata gaatagctaa ggtaaaaatt
     6661 gtactcagta ctctcccaag aaattcgctc tcaaccttgg tttgtacttg agtaaaaaag
     6721 tgaatattaa aaatcgtcat aaacaattca caaactaaat ttccagaaaa ggaaagaaaa
     6781 gttggaagtg gtaatcccat cataaaaact ccgacacctg tcaaagccag taaaatcaaa
     6841 agattataaa tattagcttt aattttacta gctagaagag ccccaatgat ggaaccaata
     6901 gcccccatag ttaaaatact tgcataggct ccttctgacc cgtaaagctg attcgaaaag
     6961 ggaagtagaa attcaaaagc tgcaaaaaag aaattaacgc tggaagctac cagcaaaagg
     7021 aagaaaattt cttgctgatg ccagatatag tgtaacccat ccttgatatc tacaaaaata
     7081 tctctcccag taaaagcctt tttctcttga acttttgctt cctcttttgg aaggaaagcc
     7141 actagaacaa aagcaatgaa aaaaggcagc gagtctagca gtagcgtcat atggagactt
     7201 gcaaactgta aaacaaggaa ggaaagaaca ggagagctaa cacctacaac ctgcaaaacc
     7261 agctctaagc gagaattata gatcacaatc tcatctttct ccaccacttc agttatgata
     7321 gctttattgg ctgtgcgaga aaaggcaaaa gcaatagcct gcacaatgtt agcaacaatc
     7381 aaagcgccaa tcatccagct atcattcctt atgaaagaaa tagccagaca aagaatccca
     7441 caaacaagat ctgccgtcat taaaatctta cgacgagaaa aacggtctga aataactccg
     7501 ccaaagggat tgacgagaat agatgtgacg agctcagaaa tctgatacat tcctaaaact
     7561 gtctgtccta tagtccccat agaagccaac cagacactat ttccataatc atagagcata
     7621 tttcccattt tattgatagc cccacggcta atcaactgca ctgcatagcg attcatatta
     7681 aagctcctct caaattttga aactattgta tcaaaaccga aaggagcttt ttattttttc
     7741 ccttatttgg gaaaattaac ttttgacaaa tttttcgtag tgttcctgat aataggctac
     7801 ttgctctgga agacctaaca catcaaaaat atgtatggcc tcttgcatct gcttacagcc
     7861 ttctttacac tgtccttttt gatataaggc aaaacctttt aaataatgga aaacattacg
     7921 ctcataaagc ttaatatctt tgtcaataat cttctctgta taagcctcaa aatagttggc
     7981 attataaaaa gaagaatgct ctaaacaatg ctggtaacaa ttgagggcca aaattaacac
     8041 taatctctta tggcgactaa tctcttggta aaattcctcc ctctccataa cttctctacc
     8101 aatccgagtg acatagtcta catcgtagaa actatagagg ttaccgaaaa gaatcaactc
     8161 atacatggtc cattcttctg ttttgaagag ataatctgct accttatcca aatcatcctg
     8221 cttcatatca taagtcgcat ctctttgaca aatcagacct tgtagcaaaa tccagttcag
     8281 ctcaaaataa aggggagtcg tcgaactctt agacttttca agttgttctc tttgaagctt
     8341 ttgaaaacct gcaatatcgt ttgaatagta aagtgggata atctgtgcca tcatagacac
     8401 atgttcatga ttatgaaaat tccttgcctt atccatgaaa ttttcgattg ttacatgaat
     8461 gttatccaaa atctcaaaga aacgggagac tgccaggtca gactccccaa gctcaaagcg
     8521 agataactga gaggtagagc aggattcgcc tgctgcttcc tttaaagaat aatttccact
     8581 tgttcgaaat tcacgaaata cttttccaag atgttccatt tttacacctg ctctgataat
     8641 tcttcccact caagcatagc ttcttcctga cgatggctga ttttgtccag ctcagcctgt
     8701 aattccatga gtttgtcggc atcgtttgtt tccaacattt gttcagaaat ggcttggctt
     8761 tgactttcta gctcttcaat ttcagcttct agactttcga tttgtcgcat gagtttgcga
     8821 acttcttttt gactttcttt ctgggcctga tagtcattga ctggacttgc ttcctttgct
     8881 tgattgctag ttgaagcttc ctcagtctga ctcatttctg ctgttgctct cttctcaata
     8941 tagtagtcgt aatctccaag gtagagagtt gaaccattct cagacaattc caaaacatga
     9001 gttgccacac gattgataaa gtaacgatca tgactgacaa acagcaaggt tccatcaaag
     9061 tcaatcaagg cattttctag cacttcctta ctatcaatat ccaagtggtt ggtcggctca
     9121 tccagaatca aaaaattatt gttttccata gacaatttag ctaaaagcaa acgagctttt
     9181 tcgccaccag atagcatgcc gactgatttt ttaacatcat ctcctgagaa aaggaaggct
     9241 ccaagacggt tgcggatttc aacttctggt gtcagtttga aatcattcca gagttcatcc
     9301 agcaccgtat tacttggtgt cagcttgctt tgggtttggt catagtaacc aacctcaaca
     9361 ttagcgccaa agcgcttttc tcccttgata aaaggaatct ggtccacaat agacttgata
     9421 aaggttgact tgccgatacc atttggacca acgatagcga cagcattcat cttacgaaga
     9481 tctaggttga taggttgtga caagacttcc ccgtcataac caatagcagt attttcaaca
     9541 gtcaaaacaa cattgcccga cgttttttca gactggaagg tcatgttggc tgctttcttg
     9601 ccagcttcag gcttgtccaa acgctccatt ttttctagtt gtttacggcg agattgagca
     9661 cgtttagtcg ttgaagcacg aactagattg cgattgacaa agtcttccag agcagcgatt
     9721 tccttctgtt gcttttcata gttttttgcc tcagtaacta gcttttgctc cttcaattcg
     9781 acaaaacgag agtaattccc cacatagcga tccaaggaat gcttggtcaa atctagcgta
     9841 attgtcgcaa ccttgtccaa gaaataacgg tcgtggctga cgataaggag ggcaccgcta
     9901 tagtttacca agtaattctc tagccaggcg atggtttcaa tatccaagtg gttagttggc
     9961 tcgtccaaga ccaagagatt gggcttttca aggagcattt tggcaagtgc caaacgagta
    10021 ttttgaccac cagaaagctc agcaattttc atctgccaca tagactcgtc aaacttgaat
    10081 ccattcaaaa tcgctcgaat atcagcttca taggtaaagc cacctgcttg gcgaaaattc
    10141 tcagataagc ggtcataatc tgacatcagt ttatccaaat cctcaccaga cttttcaccc
    10201 atctccagct ccatctgacg cagttgtctc tccgtccgac gcaaattatt aaagacatga
    10261 agcatttcat cgtagatggt attttcagac tcaaaacggc tatcttgggc taggtaagac
    10321 agagaaatat cttttttctt attgatttct ccgctagttg gctcctcttc tccaactaaa
    10381 atcttcaaaa gagtagactt acctgcacca tttttcccaa caagggcaat ccgatctcgt
    10441 tcatcaacct gcaggttgat attatcgaaa agaacctctc ctgcaaaaga acgttcaatt
    10501 ttattagctt gtaaaataat cataagagta gtatagcatg tttccctaag gcattcaaga
    10561 t
//
LOCUS       DAVKQL010000066        10529 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVKQL010000066 DAVKQL010000000
VERSION     DAVKQL010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10529
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.066"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(<1..668)
                     /locus_tag="VW561_001779"
     CDS             complement(<1..668)
                     /locus_tag="VW561_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9944006.1"
                     /translation="MSEYWFSTNVDQIDEVDGKQCLIYSYYNIKASRNVEVLKGRSGT
                     KKGLDYWEPYAPQKQYEMERLPKNKYIGSSSTDRWDGIEKNVVFCDCKEYVSAFDLFF
                     YHYNFKKISTQRSKQDFIRLRSKPVADILKNNTSSYTRYKKEMVIDNVKVDDKVCEII
                     SEIMDESYTDIQILTHKLYSKGDDIKASKTIWMKKSGKEYSEAFAGTGEARIILLVND
                     IVNA"
     gene            complement(877..1968)
                     /locus_tag="VW561_001780"
     CDS             complement(877..1968)
                     /locus_tag="VW561_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEU9944007.1"
                     /translation="MGKIVAIDLFSGAGGTTSGLKKSGIDVQVAVEIDSVAVKTYKLN
                     NPEVSVIDKDINVVSGDEVKEYLKIDTDDKLMLVACPPCQGFSTIGTNNENDERNQLV
                     FQFLRLINELNPDFLLMENVVGMTRTRNEKIFKKFLTSIDNNYSVIYDILNTADYGVP
                     QSRKRLVLHGIRKEILDNTNLTLNLPSKTHSNIPELDLLSWINADIIMDLPPIEGGEE
                     YIGEGIYNHVANKLSNLNIERITHIRNNGGSRTSLPKSLELNCHKNRLGHSDVYGILD
                     INKPSITITGGCMSYSKGRFGHPTQNRALSAREAARLQSFDDDYIFVGNRGQLAKQIG
                     NAVPVKLAQASGNYFMSVYREIANGQSDN"
     gene            complement(2049..4700)
                     /locus_tag="VW561_001781"
     CDS             complement(2049..4700)
                     /locus_tag="VW561_001781"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEU9944008.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ITRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAMANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNEAGEIYVGEEAPEGDDWTQDSDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLPLEQATANVEKVATGEAGNVTDRWILHNLNETIGKA
                     TANFDKFEFGVAGHILYNFIWDEFADWYVELTKEVLYSDNEEEKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAAYPTVNLAFEDLAAHTGVESLKNLIRAVRNA
                     RAEVNVAQSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIYLPLADLLNVEEELARLEKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     ERDKQADYQAKYDATVARIDEMKKLVK"
     gene            complement(4693..5271)
                     /locus_tag="VW561_001782"
     CDS             complement(4693..5271)
                     /locus_tag="VW561_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9944009.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLMDSTLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYHLIYFKQIQSLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKYKPLKRHIEVSEREIIETAETLQLNSQHQPNELNH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            complement(5271..5831)
                     /locus_tag="VW561_001783"
     CDS             complement(5271..5831)
                     /locus_tag="VW561_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9944010.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHILPERNQKENLPAGYGIVVKVTDKIVGSVDFNHRHEDDVLEIGY
                     TLHPDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSKRVAEKLGFTLEARI
                     RDRKDAQGNRCDDLRYALLESEWEVI"
     gene            complement(5818..6267)
                     /locus_tag="VW561_001784"
     CDS             complement(5818..6267)
                     /locus_tag="VW561_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEU9944011.1"
                     /translation="MIGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEK
                     LERLGVHYEISERTQIPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPELLDE
                     KGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            complement(6680..7075)
                     /locus_tag="VW561_001785"
     CDS             complement(6680..7075)
                     /locus_tag="VW561_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEU9944012.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLDLKGIGPATIKKLKENGIKFK"
     gene            complement(7171..7425)
                     /locus_tag="VW561_001786"
     CDS             complement(7171..7425)
                     /locus_tag="VW561_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEU9944013.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKVGKGFHDLPEGLFGERNY"
     gene            complement(7435..8769)
                     /locus_tag="VW561_001787"
     CDS             complement(7435..8769)
                     /locus_tag="VW561_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEU9944014.1"
                     /translation="MTDERIHILRDILLELHNGASPESVQDRFDATFTGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYESMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQIFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKAIMKGLREGTKDKY
                     EMWFKSESRGKFVHITYAAVHDENGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            complement(8769..9002)
                     /locus_tag="VW561_001788"
     CDS             complement(8769..9002)
                     /locus_tag="VW561_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEU9944015.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            9033..9107
                     /locus_tag="VW561_001789"
                     /pseudo
     CDS             9033..9107
                     /locus_tag="VW561_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(9316..9468)
                     /locus_tag="VW561_001790"
     CDS             complement(9316..9468)
                     /locus_tag="VW561_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944016.1"
                     /translation="MKFFWGLLAILFIKPIIGIVKFFWMIISFAVQLLFYKIVFKILD
                     WLFKLI"
     gene            complement(9465..9647)
                     /locus_tag="VW561_001791"
     CDS             complement(9465..9647)
                     /locus_tag="VW561_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944017.1"
                     /translation="MGLIKTLAKIYGNYFLTVQGVKVMKTIKKDDNAVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            complement(9649..10428)
                     /locus_tag="VW561_001792"
     CDS             complement(9649..10428)
                     /locus_tag="VW561_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944018.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELGPQDIPTL
                     LEQGPTSLLSQEILDRVANACIRDNVLLASGTSVLAGLPGGLAMAITIPADVAQFYGF
                     SLKLAQELGYIYGYEDLWASRDELSEDAQNTLLLYLGVMLGVNGTAALLRAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLRIFGVNLTKGGLAKGMGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIEGE"
BASE COUNT         3040 a         2334 c         1894 g         3261 t
ORIGIN      
        1 tgcgcattta ctatatcatt tactaacaaa ataattctag cctcacctgt tcctgcaaaa
       61 gcctcggaat attcttttcc agattttttc atccaaatag ttttggaagc ttttatatca
      121 tcaccttttg aatacaactt atgtgttaaa atttgaatgt ctgtataaga ttcatccatt
      181 atttcactaa taatttcaca aactttatca tcaactttaa cattatctat aaccatttcc
      241 tttttataac gcgtatagct acttgtatta ttctttaaaa tatcagcaac tggtttagat
      301 cgtaatctta taaaatcttg tttactacgt tgagtagaaa tttttttaaa attatagtga
      361 tagaaaaata aatcaaaagc agaaacatat tctttacaat cacaaaagac aacatttttt
      421 tcaatgccat cccatctgtc tgtcgaagaa cttccaatat atttattttt gggtaatctt
      481 tccatctcat attgtttttg aggagcatat ggttcccaat aatctaatcc tttttttgtt
      541 ccagaacggc ctttaagaac ttctacattt ctagaagctt taatgttata atatgaatag
      601 attaaacatt gtttcccatc cacttcatct atttgatcaa catttgtact aaaccaatat
      661 tcagacacac ttttattggc tggagaacca tataaagctt gtaaaattga agttttattt
      721 actccatttc taccaactaa taaagtaata gggaaattaa aatttattct tgaatttaat
      781 gataagctct tgtaatgagg aaatcgtata taatcaatat aaggataaaa ttgattttta
      841 tttttaaaaa aatttttagt tattccttct aaactctcaa ttatctgatt gtccatttgc
      901 tatctcccta taaactgaca taaaataatt tccactagct tgagctaatt ttactggaac
      961 tgcattacca atttgcttag ctaattgacc tctatttcca acaaaaatat agtcatcatc
     1021 aaaagactgt aatcttgcag cttctcttgc acttaaagct ctattttgag ttgggtgccc
     1081 aaatctacct tttgaatatg acatacaccc tcctgtaata gtaatggaag gtttgttgat
     1141 atctaatatt ccatatacgt cactatgacc taaacgattt ttatgacaat ttaattctaa
     1201 agattttggc aaagaggttc tacttccccc attatttcta atatgagtaa ttctctcaat
     1261 atttaaatta gatagtttgt ttgcaacatg attgtagata ccttctccaa tatattcttc
     1321 accaccttca ataggaggca agtccattat tatatccgca ttaatccatg aaagaaggtc
     1381 caattctggt atatttgaat gagttttgga aggcaaattc aaagttaaat tcgtgttatc
     1441 aagaatttct tttctaattc cgtgtaacac caatcttttt ctagattgag gaactccata
     1501 atcagctgta tttaaaatgt cataaataac actataatta ttatcgattg atgtcaaaaa
     1561 ttttttaaag attttttcat ttcttgttct agtcataccc acaacatttt ccattaatag
     1621 aaaatcagga ttcaattcat taatcaaacg aagaaattga aaaacaagtt gattcctctc
     1681 gtcattttca ttattagtac caatagttga aaaaccttga catggtgggc atgccacgag
     1741 cattagttta tcgtctgtat caattttaag atactctttt acttcatctc ctgatactac
     1801 atttatatct ttatctatta cagacacctc aggattattt aacttataag ttttaacagc
     1861 tacggaatca atttcaacag caacttgaac atctatgcct gattttttaa ggccacttgt
     1921 agtgccacct gcaccgctaa ataaatcaat agcaacaatt ttccccatag tattctccta
     1981 aagtttctcc tttttattat aacattatca aatgtaaaac ccaacccgat agggttaggt
     2041 ttttaacatc atttcaccaa cttcttcatc tcatcaatac gtgctacggt cgcatcatac
     2101 ttggcttggt agtcagcttg tttgtcgcgt tctttttgga cgacttctgg tttggcgttg
     2161 gctacgaagc gttcgttaga gagtttctta ccaaccatgt ccagttcttt ctgccatttg
     2221 gcaagttctt tttccagacg tgccaattct tcttcgacat tgaggagatc ggccagtggc
     2281 aggtagattt ctgctcctgt gatgacactt gacatagcca gttcaggtgc agggatggtt
     2341 gatgcgattt ccaagtgttc tggatttgta aagcgtttga tatagttgac attgctgtta
     2401 aagaaggctt ccaagtcgct atcgcttgtc ttaacaagga tggtgatagg tttgctttgt
     2461 gctacgttta cttccgcacg cgcattccga acagcacgaa tcaagttttt gagactttcc
     2521 acaccagtgt gagccgcaag gtcttcaaag gctagattaa cagttgggta tgcagctgtc
     2581 acgatagaac cttctgagat ttgtccaaag atttcctctg tcacgaatgg catgattggg
     2641 tgaaggagac gaaggatctt gtccagagtg taaaggagaa cagaacgtgt gatgactttc
     2701 tcttcttcat tatcgctata aaggacttcc ttggtcaact caacatacca gtccgcaaat
     2761 tcatcccaga tgaagttgta gaggatgtgg ccagcaacac caaactcaaa cttatcaaag
     2821 ttagcagttg cttttccaat cgtttcattg aggttgtgga gaatccagcg gtccgtcaca
     2881 ttaccagcct cacctgttgc aactttttcg acattggcag ttgcttgctc aaggggcaaa
     2941 ccttcattgt tcataaggat gtagcgagag atgttccaga tcttgttaat gaagttccat
     3001 gaagcatcca ttttctcata agagaaacgc acgtcttgtc ctggcgcaga accgtttgaa
     3061 aggaaccatc gaagggcatc ggcaccgtat ttctcgataa catccattgg gtcaatcccg
     3121 ttaccgagag acttagacat cttgcgtcct tgctcgtcac gaataagacc gtggataagg
     3181 acgttttgga atggttgacg gcctgtgaat tccaatgact ggaagatcat acgagacacc
     3241 cagaagaaga tgatgtcgta acctgttacc aaggttgaag ttgggaaata acgtttaaag
     3301 tcttctgagt cgacttcagg ccagcccatg gttgaaaatg gccaaagagc agaactaaac
     3361 cacgtatcaa gcacatctga atcctgagtc cagtcgtctc cttctggtgc ctcttctccc
     3421 acataaatct cacctgcctc gttgtaccaa gcagggattt ggtgacccca ccagagctga
     3481 cgagagataa cccagtcgtg gacattttcc atccattgaa ggaaggtatc gttgaaacga
     3541 ggtgggtaga attcgacctt gtcctctgtg tcttggttgg ccatggcgtt cttagccaat
     3601 tggtccatct tgacgaacca ttgagtagac aagcgtggct caactacgac acctgtacgt
     3661 tctgagtgac caacactgtg gacacgtttt tcgattttga caagggcacc gatttcttcc
     3721 aacttagcaa cgactgcctt acgagcttca aaacgatcca tgcctgaaaa ttcaaaggca
     3781 agctcattca tagttccgtc gtcgttcatg acgttgactt gtggcaagtt atgacgttgg
     3841 ccaaccaaga agtcatttgg atcgtgggca ggtgtgattt tcacgacacc agtaccaaac
     3901 tcaggatctg cgtgctcatc tccaacgatt gggatgagtt tattagcgat tggaaggatg
     3961 acgtttttac caatcaagtc cttgtaacgc gggtcttctg gattaaccgc aaccgcaacg
     4021 tccccaaaca tagtctcagg acgagttgta gcaacttcaa gggcgcgtga accatcttcc
     4081 agcatgtagt tcatgtggta gaaggcacct tcgacatcct tgtgaatcac ctcaatatca
     4141 gaaagggctg tgcgagctgc tgggtcccag ttgatgataa actcaccacg atagatccag
     4201 cctttcttgt aaaggtccac aaagacctta cgaacagctt ttgacaaacc ttcgtcaaga
     4261 gtgaaacgct cacgagaata gtctacagaa agccccatct tgccccattg ttccttgatg
     4321 gtagtggcat attcgtcttt ccattcccag accttcgtca agaaagactc acgacctagg
     4381 tcataacgcg taataccctc accacgtaag cgctcctcaa ccttagcctg agtcgcaata
     4441 ccagcgtggt ccatacctgg aagccaaagc gtatcaaagc cctgcatgcg tttttgacgg
     4501 atgatgatat cctgcaaagt cgtatcccaa gcgtgaccaa ggtgaagttt cccagttacg
     4561 tttggtggtg gaatcacgat tgaataaggc ttagcctttt gatcgcctga aggcttgaaa
     4621 acatccgcat caagccattt ttggtaacga ccagcctcaa cctcggctgg attgtattta
     4681 gatgaaagtt ctttagacat gtgtgtgtcc tttctctatt ttgtttattt tattttgaat
     4741 ctgcttagca gcttcttctg cagacaaatt cgtattattt attttaaagt agtggttcaa
     4801 ctcattcggt tgatgttggg aatttaattg aagtgtttca gcggtctcta taatttctct
     4861 ttcagatacc tcaatatgtc gttttaaggg tttgtacttt aatcgattct ccgttcgatt
     4921 tcgacgtatg cactcttcaa gacttgtttc caactcaaca aaaagaatct cttgatgata
     4981 atcatccaac aaactctgaa tttgcttgaa atatatcaga tgatactgat ttgaaaaatc
     5041 aattacatct gttaaaatta ctgatcgctg atttcttgca ctcgctccaa ggaatgaaaa
     5101 agtgattcca cgaacaaatt cccacatttc ctctgtatag tcctgataga tttctagtgc
     5161 aaaatcgatg gcttgatggt tataaaagag ggtagaatcc atcagtcgag acagttcttg
     5221 accaatggtc atttttcctg atgctggagc accaatgatg aaaagatgca tcaaatcacc
     5281 tcccactcac tctccagcaa ggcatatctc aaatcatcac agcggttgcc ttgagcatct
     5341 ttgcggtctc ttatgcgagc ttcgagggta aagccaagct tttccgcgac tcgtttactt
     5401 tgaaggttat atccaaagca agttagttca atcttgtgaa gacccaaatc tttaaaggct
     5461 aagtcaatca aggcacgcgc agcttctggc acataacctc gcccccaata gtctgggtgt
     5521 aaggtatagc cgatttccag cacatcatct tcatggcgat ggttgaaatc gacagagcca
     5581 acgatcttat ccgttacttt gacgacaatc ccgtagcctg ctgggagatt ttccttttga
     5641 ttacgctccg gaagaatgtg ctccagataa taaatctcat cttccaaggt cttgactgga
     5701 ggaaaacctg ctggataggc gacctctggc aaactagcat agtcaaagat atcctcagca
     5761 tccgccacag tacggactcg taaaacgaga cgttctgttt cgattttatc tggcagctca
     5821 gttcttgcca tccttcttcc tcgctttttt gatgaaactg cccttcatat ctacacgctt
     5881 atccagatag cgataaacgc gctgatatcc atctcccatg aaataggttg gggcaaacag
     5941 ttgattttta aaatgtccct tttcatccag gagttctggg gcaacaagtc gctcaagaat
     6001 cttggcaaag atgtggcaaa taccgtcttc ctcaacaatc ctatctaccc gacaatctaa
     6061 aacaagtgga caggcgtcta aaataggaat ctgagttcgt tcagaaattt cataatgcac
     6121 tcccaaacgt tccaatttct cctgatgact gataaaacca gcctgctcca tcgcaagcat
     6181 agaagtttca tcagaaatat tcacagtaaa tttttgatac tgtttgatct gctctgcggc
     6241 attctctctc gcaacgactc caatcacaac ccaatctcct agactataag aggaactaca
     6301 ggtcgtgatg ttatagccaa aattctaatc ttgatatcct aaaataaaaa caggaaaacc
     6361 ataatatagt ttacttgtgt taaaagattg cttcataaca accccctttg actaagacgt
     6421 aaaagaaaag ccctgccatc tacatgacag ggacgaatgt gtttatccgc ggtaccaccc
     6481 aatttcgggc agttgcccgc aactctcgtc tttaaagcaa aaagacactt ttatttcaat
     6541 ttttcatcca ttagcaacca gttttgacac atttgtggac ttctcagcgc cgccactttc
     6601 tgtaaaatgt gggattcaaa acacctctaa tagctctatt gtagcaagat tttttcggaa
     6661 atgcaaggct caagaaaaat tacttgaact tgatgccatt ttctttcaat ttcttgatgg
     6721 tagctgggcc gattcctttc aagtcaagga gttctttttc agtccagttt ttgaaatctg
     6781 aagcagactt gattccttca tcatagaaag tcttggcacg atcaagtgga agtccaccca
     6841 agtttgctgc aaaatcttct acagaattag ctactttttg agcttgctct ttggtagctt
     6901 ctactgcttg ttcaactttt ttagaaacag ccttaccagc tgcgcttgct gactgttctg
     6961 cacgcttcac gacttttttt gtctcatcta caaccttagt cacagtactt gaaaaggcac
     7021 ctgcgcgacg gaggccattt cgtaattgtt ttttacgatt gagtttcttt gacatgataa
     7081 aatcctttca ataaaaaatc cctgctctga caaggattta acataaacat tctatcaaga
     7141 ttttagtgaa aaatcaagaa tctttctcgt ttaataattt cgctcaccaa acaagccttc
     7201 tggcaaatca tggaatccct tgcctacttt gaagttttca aacttaccaa gcaagaactg
     7261 ataagcatcc aaacgactct cgtagcgaat catggcatct ttggcacgct cgtcctggtc
     7321 ttcttcgtaa actttttgac tttccttgtg cgccatgtcg ttaatcatga tttgggtatt
     7381 gacccaagct tcaaattcct tcataaattc ttgttcgtaa ctcatttttt ctccttattc
     7441 taatccacgg aaatagtccg tatcaatctc acggtagggc tggatatcct gaacgtattc
     7501 cagtacacct tggaattctc cgttttcatc gtgtactgca gcataggtga tgtggacaaa
     7561 ctttcctcgc gactcagact tgaaccacat ttcatacttg tccttggtcc cctcacgaag
     7621 tcccttcatg atagccttga ccttgtccaa atacttaggc ggatgacaga gttcgacatt
     7681 gcgcccgact tgggacggcg tccgtttgaa aatcatctca tcagctggcg tattgtcatt
     7741 gtaatactgg aaaatatctt ctttattgac aaaggtaatc tccataggga gatgattgag
     7801 gatgagattg gcctgctcga ctgaaagata gccattacca aaagcctgtt gactatggcg
     7861 gtccagcaca gcttcctttt ccttaggggt aaaggtaatg gtaaaatggc cttctggcgt
     7921 atctatgact tgttgaactt gaccttctgc cgtatctagt tgtacaggct cctctgcaat
     7981 cttttcctca ataaagatct gtcgttctgg cacccatttc tctgacggac ggatgatggc
     8041 atagccatag gcatcactct cctccgcaat ctgaagccag tcatcctgag taaaagactc
     8101 aaggagaatc atgagcagga tggactcttc cttgaaaatc atactttcaa actctgtcgc
     8161 aaaagcttca aaagcttcct ttacactgct aattgacact tctggtagtg acttggctgt
     8221 cgttagagct gtttgaaaga gttccctaat ctgatcatcc actccccaca taactttggg
     8281 aggtgaatcg tgtccatagc gctccatgat aggaaagaag agttcttcct tacgttggta
     8341 atggatgtca aattgaccca caagtcccat ctgacgcacc aaacccttac gcatctccgc
     8401 cagcatttcc tcgtcttcca tagactcata ggtatctaac aatctacgaa tgcgaatcaa
     8461 ggccgcacgg agagccagat tttcttcttt gaagacacga actgggtgac ctggatgctc
     8521 agtatctgaa acttcgacac ctttgatagc atttttaaaa agattggcat ggacatcaca
     8581 gagttccata acatcttcaa aagtgacgcc cgaatccgag ttcatcagct cgtgctccat
     8641 aagggaaatc tcgatggctg acacgcccgt aaaggtcgca tcaaagcgat cttgaaccga
     8701 ctcaggagag gcgccattgt gcaattctaa caaaatatcc cgtaggatat gaatccgttc
     8761 atctgtcatt agtctaatcc aatcacttcg tagccattcg cttccagtgt gcgtacaatc
     8821 ttgtccatag gagttcctgc tagcttagaa ccctgtttaa gtgatacttt acgaccaact
     8881 gtattgcgca ttaagggatt ggcaaggggt ttaaaaccca actccactag aatttccaag
     8941 acttctggat gcttgtccac cacttctgca acaggaattg acacatcgat gatattgtcc
     9001 atgacgacct ccattgtatg ttctaatact caatgaaaat caaagagcaa actaggaagc
     9061 tagccgcagg ttgctcaaaa cagtgttttg aggttgtaga tagaactgac gaagtcagct
     9121 caaaacaccg ttttgaggtt gtggatagaa ctgacgaagt cagtaacata tatactgcaa
     9181 ggtgaagctg acgtggtttg aagagatttt cgaagagtat aaaatgattt tactgcttat
     9241 attataccat atggaaagag aataaaaaaa ctagcagtgg agttcctgct agttctctgc
     9301 aacttggatt accatctaga taagtttaaa gagccaatcc aatatcttaa acactatctt
     9361 gtaaaacagc aattggactg caaaagagat gatcatccaa aagaatttca caatcccaat
     9421 aatcggtttg ataaaaagaa tagcaagaag accccagaaa aatttcattc tctctcctct
     9481 ggcttaatga gccaccgagc cgtatccatt aacttgtcgg caataaaaag tttccccaga
     9541 ccgacaacgg cattatcatc tttctttatc gttttcatta cttttacacc ttgcacggtc
     9601 aaaaagtaat taccataaat tttagctaga gtttttatga gtcccatatt actctccttc
     9661 gatgatttca gcctcttttc ggattgtttc aacatcttct tgatattgaa cttcactata
     9721 gttaactagc ttggacaatt ctttctgcaa gctttcaccc atcggtttca tagttgcaaa
     9781 ggttaaacca cctgaaatga taccacccaa gataggaata aatttcccca ttcctttggc
     9841 cagccctccc ttggtaagat tcacaccaaa tattcttaag acttttttca aaataggata
     9901 ccaaagcgtc tttgttaaag ctttattagg tactgttttc attacctgtt tggcaattgt
     9961 tataccacca gcacgtagca aagcagcagt tccattcacc cccaacatga cgcctagata
    10021 aagcaaaagg gtattttgag catcttcact caactcatcg cgtgaagccc aaagatcctc
    10081 ataaccataa atataaccta attcttgggc caatttcaga gagaaaccat aaaattgagc
    10141 cacatcagct ggaatggtaa ttgccatagc aagccctcca ggtaatcctg ccaaaacaga
    10201 agtcccactc gctaataaaa cattatccct aatacaggca ttagccactc gatctaagat
    10261 ttcttgagat aagagagacg ttggaccttg ttccaataag gtaggaatgt cctgtggacc
    10321 caattctttg gaaaacttat ccactaaaaa tttcgaacga tcaaccttaa caactggtag
    10381 ttttaccact tgttgtaata cttgcatagc taagtcttgt tcagccatat acaaactcct
    10441 tatttctttt ttacacctta tcatattata tttcctacaa aaaaagcatt ttctaattag
    10501 ataacattaa aaaatacaga acccctttt
//
LOCUS       DAVKQL010000067        10511 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVKQL010000067 DAVKQL010000000
VERSION     DAVKQL010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10511
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.067"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            1..516
                     /locus_tag="VW561_001793"
                     /pseudo
     CDS             1..516
                     /locus_tag="VW561_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            416..862
                     /locus_tag="VW561_001794"
     CDS             416..862
                     /locus_tag="VW561_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9944019.1"
                     /translation="MRKHGLISKSTSKRYYQVAIPFTRLFCPTLVSIDYINCLSETID
                     KNWHKSDRIFVTNTGKPVHSSILSKSLQRANERLKKPIPKHLSPHIFRHTTISILSEN
                     KIPLKTITDRVGHSDSEVTTSIYTHVTKNMKDEAINVLDKVMKKIF"
     gene            complement(1000..2304)
                     /gene="eno"
                     /locus_tag="VW561_001795"
     CDS             complement(1000..2304)
                     /gene="eno"
                     /locus_tag="VW561_001795"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU9944020.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            2468..2914
                     /locus_tag="VW561_001796"
     CDS             2468..2914
                     /locus_tag="VW561_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEU9944021.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(2911..4026)
                     /locus_tag="VW561_001797"
     CDS             complement(2911..4026)
                     /locus_tag="VW561_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEU9944022.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVEYLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     ASGQSLLNLASVSTENCYKIPEGVQIHILADVVSPLCGHQGATYTFGNQKGLHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGSGGGLAGGLCAFAQASIVSGIDTCLDLINFNKKV
                     SDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASLYLERTAANIGHLLNMYKI"
     gene            complement(4243..4614)
                     /locus_tag="VW561_001798"
     CDS             complement(4243..4614)
                     /locus_tag="VW561_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEU9944023.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQLA"
     gene            complement(4673..6106)
                     /gene="glgA"
                     /locus_tag="VW561_001799"
     CDS             complement(4673..6106)
                     /gene="glgA"
                     /locus_tag="VW561_001799"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEU9944024.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGLERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(6103..7242)
                     /gene="glgD"
                     /locus_tag="VW561_001800"
     CDS             complement(6103..7242)
                     /gene="glgD"
                     /locus_tag="VW561_001800"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEU9944025.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAEMFNMSTDIFVVDTPWLIEHLEEEAKKEHPKK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GKGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(7232..8374)
                     /locus_tag="VW561_001801"
     CDS             complement(7232..8374)
                     /locus_tag="VW561_001801"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEU9944026.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGAVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(8364..10292)
                     /gene="glgB"
                     /locus_tag="VW561_001802"
     CDS             complement(8364..10292)
                     /gene="glgB"
                     /locus_tag="VW561_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEU9944027.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPV
                     AVRYEARPGTGAIVTELPDKKWRDGLWLARRKRWGFAERPVNIYEVHAGSWKRNPDGS
                     PYSFAQLKDELIPCLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDNAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         3205 a         2311 c         1863 g         3132 t
ORIGIN      
        1 aaccacacct actatgaaca agacccagaa aaagtagcca tatttcttaa gaattttaat
       61 agattaaagc acctagcacc tgtttagatt gacgaaacag gattcgatac ttatttttat
      121 cgagaatatg gtcgctcttt aaaaggtcag ttaagaagaa gatatcagag gatttctttg
      181 gttgcaggtc taaccaatgg tgagttaatc gctccaatga cttacgaaga gacgatgacg
      241 agcgactttt ttgaagcatg gtttcagaag tttctcttac caacattaac cacaccatcg
      301 gttattatta tggataatgc aagattccat agaatgggta agttagaact cttgtgtgaa
      361 gagtttgggc ataaactttt acctcttcct ccctactcac ctgagtacaa tcctattgag
      421 aaaacatggg ctcatatcaa aaagcacctc aaaaaggtat taccaagttg caataccttt
      481 tacgaggctc ttttgtccta ctcttgtttc aattgactat ataaattgtc taagcgaaac
      541 aatcgataag aattggcaca aaagcgaccg tatttttgtt accaatacag gaaaaccagt
      601 tcatagttct atcttgagca agtctctcca gcgagcaaac gaacgcctta aaaaaccaat
      661 tcccaaacat ctgtcccctc acatcttcag acacaccact attagcatct tatcagaaaa
      721 taaaattcct ttaaaaacaa tcacggacag ggttggtcat tccgactctg aagtcactac
      781 ttccatctac acccacgtca caaagaacat gaaagatgaa gcaatcaatg tactggataa
      841 agttatgaaa aagatttttt aaaaagtttt gtcccttttt tgtcctctaa atacaaaaat
      901 agcccttcgg ataaaatccg aggagctaga aacgttgtta aatcaacggc cgaacttttg
      961 aatttcatgg ttcgggataa aatagttcac tgaactattt tattttttaa ggttgtagaa
     1021 tgatttcaat ccacgatatt cagctacttc accaagttgg tcttcgatac gaagcaattg
     1081 gttgtattta gcgatgcggt ctgtacgtga aagtgaacca gtcttgattt gtcctgcgtt
     1141 agttgcaact gcaatatcag cgattgttga atcttcagtt tcacctgaac ggtgtgatac
     1201 aacagcagtg taaccagctt ctttagccat ttcgatagct tcaaaagttt cagtaagagt
     1261 accgatttgg ttaactttga taaggattga gttagcagca ccttcttgga taccacgtgc
     1321 aaggtagtca gtgtttgtta cgaagaagtc gtcaccaaca agttgtactt tcttaccaag
     1381 acgttcagta agagctttcc aaccatccca gtcgttttca tccataccat cttcaatagt
     1441 gatgattggg tatttgttaa ccaattcttc aaggtagtcg atttgttctg cagatgtacg
     1501 aacagcagca ccttcacctt caaatttagt gtagtcgtaa actttacgtt ctttatcgta
     1561 gaattctgat gaagcacagt caaatccgat aaatacgtct ttacctggta catatccagc
     1621 agcttcaatc gcagcaagga tagtttcaac accatcttca gttccttcga aacgaggagc
     1681 gaatccacct tcgtcaccta cggcagtttc caaaccacgt gatttaagga ttttcttaag
     1741 agcgtggaag atttcagcac cgtaacgaag ggcttcttta aatgttggcg caccaactgg
     1801 caagatcatg aactcttgga aagcgattgg agcgtcagag tgagaaccac cgttgatgat
     1861 gttcatcatt ggagttggaa gaactttagt gttgaatcca ccaagatagc tgtaaagtgg
     1921 gatttcaagg tagtcagcag cagcacgagc tacagcgata gacacaccga ggattgcatt
     1981 cgcacccaat ttacctttgt taggagtacc gtcaagtgcg atcatagcac ggtcaatagc
     2041 ttgttgatca cgtacatcgt agccaatgat agcttcagca atgatgttgt ttacgttgtc
     2101 aacagctttt tgtgtaccaa gaccaccgta acgagatttg tcaccgtcgc gaagttcaac
     2161 tgcttcgtgt tcaccagtag aagctcctga tggaaccata ccacgtccga aagcacctga
     2221 ttcagtgtaa acttctactt caagtgttgg gttaccgcgt gagtctagga cttcgcgagc
     2281 gtaaacatca gtaataattg acatttttta ctctccttat gagttaaatt ttttacacct
     2341 ctataatacc ttaaaacccc tcctttttca agaaaaaacg ttatctttgt gcaacttttc
     2401 cttaacttta taaagtaatc gctttctttt gtctgtttta ttctaacttt tatgatatac
     2461 tgttttcatg acagatttat caaaacaatt acttgaaaaa gctcatggtg ggttaaaaat
     2521 aaatccggat gagcaaagac gctatcttgg tacttttgag gaaagagttc ttggatatgt
     2581 agatattgac acagcaaata gccctcagtt agaaaaaggc tttttattta ttttagaaaa
     2641 ccttcaggaa aaagcagagc cactatttgt gaagatttca ccaactatcg aatttgataa
     2701 gcaagttttc tacttaaaag aagcaaaaga aactgatagt caagccacca tagtatctga
     2761 agagcatatt acttctcctt ttggcctggt tattcatagc aatgcaccag ttcaagtaga
     2821 agaaaaagac cttcgacttg cttttccaaa actttgggaa gttaaaaagg aagaaccagc
     2881 caaaacatcc ttatggaaga aatggtttag ctaaatcttg tacatattta ataagtgccc
     2941 aatattggca gccgtgcgct ccagatagag actggcattt tttaaactat cttctaaagg
     3001 ttcacttttc tccaaaatag aaaagacagc ttggatattt tcaaatggta ggggaggtaa
     3061 atcttcagca agactaccgc aaatagcaat aacaggaact ccaacagggg ttctttttgc
     3121 aacacctata ggcgctttcc cagcaaagct ttgactatca agccttcctt ctccaacaac
     3181 aaccaagtca gcatctgaaa ctttcttatt aaagttgatt aagtccaagc aggtatcaat
     3241 tccagacacg atacttgcct gagcaaaggc acacaaacca ccagcaaggc ctccacctga
     3301 tcctgctcct ttaatttcta atgttgcagg tgagaatttt tcataaaaat cttggatcgc
     3361 ctgatctacg actgcaaaca tagtcggatg tagacctttt tgattaccaa aagtgtaagt
     3421 cgcaccttga tgaccacata agggactcac gacatctgct agaatatgaa tttgaacacc
     3481 ttcaggaatt ttatagcaat tttctgttga aacagaagct aagtttaata aggattgacc
     3541 ggaagcaggc aagacatttc catccctatc ataaaattga taacctaaac cagcagcaat
     3601 ccccagtcct ccatcattac tggccgtgcc accaacaccg atataaatat ctttaatccc
     3661 tttagaaatg agatggagaa tcaactctcc aataccacaa gtttggattt gaagtggatt
     3721 tcgcttctct agcggaattt ttccaagacc aaccaaatca gctacttcaa atagtgccag
     3781 ttcccctttt tgaaaatagc gcatggcttc tttttgtcca aaagggtctg tcacttggat
     3841 ccatttttct tttaggtcaa gagaatgtcg gatagcatct acagtacctt ctcccccatc
     3901 accaacaggg cagaggagat attctacatc tgctatcgat tgttggaagc ctctttttat
     3961 tgcttcagct acctgttgag ctgtcaagct ttccttaaac gaatccggtg caattacaat
     4021 cttcatattt tccctcattc taaacggtca atcaaaggaa gaacttctaa aaaatccctc
     4081 ttgtcaacat gatgtggtat ttcttttttg agcacttctt tggcacaaaa ggcgattcct
     4141 aactccgccg acttcaacct ccatttttcc agagtctctt ttttgacctg gggacttata
     4201 atttgtccaa ctaattttcc tgttaaaaga ccttctttga cttcaagcta gttggcagtg
     4261 aaataggcaa taccaaggga ttttgctaat ctctccaact attggtgtaa atccaccaga
     4321 caccagacca actaggatgc cattcttttg gagaatagag atgaactctg ggacatttag
     4381 cgatagatga attgagttga agacgttatc aaagaccaaa ataggaagac cttccaacaa
     4441 ggacactctt tttcttaaac tgctttcaaa gaccaactct cctcgcattg ctcgacttgt
     4501 aatctgcgaa atttccgcct catgacctgc ctctctccct aaaagatcaa tcacttcttc
     4561 taggattaag gttccatcta catccaaaac acacaagcct tttacttgag acatcagttc
     4621 tcctctctaa acagcctaaa aatcgtatga agtcatcata cgattttatc tattaattaa
     4681 ctaaactatg gtacaagtca aggtatgact tgcaggctgt atcccatgag aagtcactct
     4741 ccatagcttg tttttgtagg tttctccaaa tgtctggatg gtttctatac aagtccaatg
     4801 ctgtttggaa agtccaattt aaccaataag gagatagatt gtcaaagcta aagccagtac
     4861 cgcttccttc gattggattg aaagcgcgaa ctgtatctcg caagcctcca acttcatgga
     4921 ccaatggcaa ggttccataa cgcatagcca tcatttgaga caagccacac ggttcaaaac
     4981 gacttggcat gaggaagagg tcacaagcag cgtagatttc ctgagcaagt ttgacatcaa
     5041 aagtgatatt tgttgatagc ttgtctgggt aaatctgagc aaaccatgag aaagctcctt
     5101 caaaggctgg atcgccagtt cccaaaagaa caatctgaac atcttcttgc aagatatggt
     5161 gaagactttc gaccaccaca tcaaaacctt tttgacgtgt caaacgagaa acaattccca
     5221 ccagtggaac gtctgctcta acaggcaagc caactctttc ttgcaatttt gccttatttt
     5281 tggctttccc agacaaatct tcctgattga aatgatagtc taaaagagca tccgtctgag
     5341 gattataaag atcagcatca atcccattca cgataccaga tactttacca gactccattt
     5401 taagaatctg atccaaatta catccaaact gactagtcat aatttcatga gcatagctag
     5461 gtgaaacggt tgaaacacgg ttcgcataga gaatacctgc cttcatccag ttcagacagt
     5521 tgttccatcg aagggtgcca tcagcgtaac gttcaaggcc aactccaaac aaatcaccca
     5581 acattccttc tgaaaattgt ccttggaatt ctaaattatg aatggttaaa actgtttcaa
     5641 tgtcctcata ggcttgaatc caacggtatt tttccttcaa caagaaagga atcatagctg
     5701 tatggtagtc atgaacatgg agaagatcag gaataaagtc aatcctttcc atagcctcaa
     5761 tggcagccag ttggaaaaag gcaaagcgtt ctccgtcatc aaaatcaccg taaacatgac
     5821 cacggaagaa ataatattga ttgtcaataa agtagaaggt tacaccattt aatactgttt
     5881 tcttaattcc acaatactgt ctgcgccaac caacgctcac ctcaaaatga agcacatctt
     5941 caatctgatt tccaaattta gcctctacca tatcatagta gggtaaaatc actgcaactt
     6001 cgtgcccagc ttttaccagt gattttggaa gagcgccaat gacgtctccc aaaccacctg
     6061 tttttgaaaa aggtgcaccc tctgctgcta caaataaaat tttcatgaat gaatatcctc
     6121 tgttacttta gcacctttct taaccacaac tggatgttct gcagttcctc gaatcacaac
     6181 accaggctca acttcaaccc ctttgtccaa gatagcatat tcgacctgag ccccttttcc
     6241 aataacaaca cgagggaaga gcaggctatc tttaaccaag ctatccttat ggacatgaat
     6301 attacgtgat agaacagaat tagctacttg accttcaata atactaccag aggcaaactg
     6361 agaagtgctt accttagatg tattagcata gtaagttggc tcttcgtttt tgacctttgt
     6421 ataaatcttt tggtttggtg agaaaagaga atagaatttt tgtgattcaa gcatatcgat
     6481 attcgcttga taataagatt taacagagtg aatattggct agatagcccg tgtactcgta
     6541 ggcgaaagct ccctctttta cagccaaatc ccgtaaaaca tagcgcaatt tctttggatg
     6601 ttctttttta gcttcttctt ccaagtgttc aatcaaccaa ggtgtatcaa cgacaaagat
     6661 atctgtagac atattgaaca tttcagctgt tgacttgcta tcaaagagtt tatgagaaag
     6721 aacatggtct gtttcatcta catccaagat tgcatttact tctgaaatat ctttcttagc
     6781 tagtttttta taaactacag tgataggctc ttttgttgta ctatgtaggt ggaaaacttg
     6841 gttcaaatca atgttaataa gaacatcgca gttgagggca accgtttggt ttgagccaga
     6901 acgtttcaaa taagtaagaa gctgttggta gtattctttt ccaactgtac tactttctac
     6961 acgggtattg taaattccta gatagtaatg actaagaagg gttgataagc cccactcgcg
     7021 tcctgaacga atatggtcaa atactgagct gatattatcc tgctggaaaa taccaaagac
     7081 actacgaaca cctgcattag caaggcttga aagtgggaag tcaatcaaac gatatttccc
     7141 accaaatggc aaacttgcta ctggacggtg gtccgtcaat gtcgacatat tgtgaaaacc
     7201 aactgtattt cctaaaatgg cagaatattt atcaatcttc atctgttgct acccccacta
     7261 cttcattata tcctacaact tgtacttcat ctgttccatc aatttcgaca ccgtcagaaa
     7321 taatcgcacc ttcaccaata atggcacgtt taatcttagc tccttgacca atgatagctc
     7381 cactcatgat aactgaatca aggacttccg ctccttcgcg aacttgcgcg cctgttgaaa
     7441 ggatagaatg tttaacagct ccatcaacga aacatccgtc tacaactaat gagtcttcca
     7501 catgagcatt tgccccgagg aagtttggtg gtgaaatcaa gtttcttgag taaatcttcc
     7561 attgacggtt acgactatcc aaggcatttt ctggagaaat atactccatg ttcgcttccc
     7621 aaagtgactc aatagtacca acatctttcc aataaccact aaattcgtaa gcataaacac
     7681 tttcacctga ctcaaggtaa tttggaatga catttttacc aaagtctgac atgccaacct
     7741 tgctcttttc agcagcgact aacatattac gaaggcgttg ccaatcaaaa atgtagattc
     7801 ccatagaagc ttttgtagat ttaggttgag ctggtttttc ttcaaattca acaatacgat
     7861 tgttagcatc tgtgttcatg ataccaaaac ggcttgcttc tttaagaggg acgtctaaaa
     7921 ctgctactgt caagctggca ttattatcct tatgagactg gagcatatca tcatagtcca
     7981 ttttgtagat gtggtcccca gacaaaatca agacatactc aggattgaca ctgtcgatat
     8041 agtcgatatt ttggtaaata gcgtgactag ttccctcaaa ccaacgattt ccttcacttg
     8101 cagaataagg ttgaagaata gagacgcctg aattaatacc gtctagtccc cagcttgaac
     8161 cattcccaat atggttgttg agagcaagtg gttgatactg tgtaacgacc ccaacattat
     8221 gaatccctga gttggcacag tttgataggg caaagtcaat gatacggtag cgcccaccaa
     8281 attgcacagc tggttttgcg atgctttgag tgagtttacc gagacgagtt ccttgcccac
     8341 cagcaagaat caaagctaac atttcatttt tcattttcta ctcctttttg gtttttattt
     8401 gtgacggttt tagtagattt caagcgacgt ttgattttcc atacacttgc tcccatagcc
     8461 ggtagagtaa aggttaaggt ctgctcataa tctttccata ggccttcttg cgtttgaaca
     8521 gtttgattat gttctttcca aacgcctccc cattcttcca actcagtatt ccatacttct
     8581 tcgtaaattc ctgcaacggg tagtccgatt gtaaaatctt tccgctcaac aggtaccatg
     8641 ttaaagatac agactaacat ttctcccttt ttacccttac gaataaagga aagaacactc
     8701 tggtctcgat tatccgcatc aatgatttca ataccatcat agctggtatc gatttcccac
     8761 agacagcgat gatctttgta aaactggttt agctgagaag tgaaatactt catcttagca
     8821 ttcattgggt cttctaggtt agaccattcc aactgttctt cagatttcca ttctaggaat
     8881 tgaccgtatt cgctacccat gaagagcaat ttcttaccag ggtgacaaat ttggtacgta
     8941 tagagattgc gcaagcctgc gaattgattg taacgatctc cccacatctt atgcatcata
     9001 ctcttcttgc catgaaccac ttcatcgtgc gagaatggca agagataatt ctccttgaaa
     9061 acatacataa agctgaaagt caccaggtta aagtcatatt tacgataaat cggatcttct
     9121 tcgtagaaac ggaggatatc attcatccag cccatgttcc atttgtagtc aaatcctaga
     9181 ccaccaatct ctttcattcc cgtaatcttg gtcgcagacg aactttcttc tgcaatcatc
     9241 atcacatctg gatattctaa cttaataact tcattcaagc gttgaagaaa ataataacct
     9301 tcatagttga gatttccgcc atctttatta ggtgtccatg gagcattatc atagtccaaa
     9361 tagagcatgt tgctaacagc atccacacga ataccatcca aatgatagac atcaatccaa
     9421 tgcttaatgc aagaaattaa gaaggactgg acttcatttt ttccaaggtc aaaattaagg
     9481 gcaccccaac catggttatg agccttatta tggtcttggt attcaaaagt cggtgtccca
     9541 tcataatagg ctaaggcatc atcgttgatg gtaaagtgac ctggtaccca gtccacaata
     9601 accccaatat tatgggtatg acactcctcg acaaaatctt gaaactcctc tggtctgcca
     9661 taagcatgct ctaaagcgaa gtaacccata agctgatacc cccaactcaa gcccaaagga
     9721 tgggacatca agggcataaa ctcaatatga gtatagttca tttcaacgag acaaggaatg
     9781 agttcatcct tgagctgggc aaaactataa ggactgccat caggatttct cttccaagag
     9841 ccagcatgaa cctcgtaaat attgacagga cgttcagcaa agccccaacg ttttcttcgt
     9901 gccagccaaa gtccatccct ccatttctta tcaggaagct ctgttacgat tgcccctgtt
     9961 cctggacgag cctcatacct gacagcaaca gggtcaatct tcatcagttg atgaccattt
    10021 tgacgtgtga catgatattt gtaaatatgc ccttcttgag ccatattggt aaagacttcc
    10081 cagaccccaa agtcatttct taccattgga atctgatttt caatccagtt ggtaaaatca
    10141 ccaaccaagt gaacagcctg agcattaggt gcccaaacac ggaaggtata gccatgctct
    10201 ccatttagtt cttccctatg tgctcctaga taatgttgga gataaaaatt ttcacccgtc
    10261 ataaaggttt ttaatgcttc tctattatcc atatactccc cttctcctgt aagcgttttc
    10321 tatgttttta ttatactacc tttttagaga agattcaagt aaattactat acttctttaa
    10381 ttattttgaa aatctacaac aagttcactt actcgttcaa ttgtaaatca atattttttc
    10441 aaaaaattgc gaaaacgcct ttctttttct actatagtga aatgaaataa aacatgcgca
    10501 aatcgatcag g
//
LOCUS       DAVKQL010000068        10404 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVKQL010000068 DAVKQL010000000
VERSION     DAVKQL010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10404
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.068"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(<1..502)
                     /locus_tag="VW561_001803"
     CDS             complement(<1..502)
                     /locus_tag="VW561_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944028.1"
                     /translation="MKQFVQFYKKDFLSVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFL"
     gene            complement(502..1266)
                     /locus_tag="VW561_001804"
     CDS             complement(502..1266)
                     /locus_tag="VW561_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944029.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKKYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLAFGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(1362..1765)
                     /locus_tag="VW561_001805"
                     /pseudo
     CDS             complement(1362..1765)
                     /locus_tag="VW561_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1945..2577)
                     /locus_tag="VW561_001806"
     CDS             complement(1945..2577)
                     /locus_tag="VW561_001806"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEU9944030.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMALPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            complement(2611..3312)
                     /gene="pyrF"
                     /locus_tag="VW561_001807"
     CDS             complement(2611..3312)
                     /gene="pyrF"
                     /locus_tag="VW561_001807"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEU9944031.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            complement(3545..4765)
                     /gene="divIB"
                     /locus_tag="VW561_001808"
     CDS             complement(3545..4765)
                     /gene="divIB"
                     /locus_tag="VW561_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEU9944032.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEESES
                     KEKEEQDKKLAKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQVSGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVVDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKRLEEEQKKQEE
                     ESNRNQTTQRSSRR"
     gene            complement(4775..5833)
                     /locus_tag="VW561_001809"
     CDS             complement(4775..5833)
                     /locus_tag="VW561_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEU9944033.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VTKVSDQKNPEPDELVDIQSHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            complement(5835..7187)
                     /locus_tag="VW561_001810"
     CDS             complement(5835..7187)
                     /locus_tag="VW561_001810"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEU9944034.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQCLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYSNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIATD
                     KNTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGLFEEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLASKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            complement(7291..7932)
                     /locus_tag="VW561_001811"
     CDS             complement(7291..7932)
                     /locus_tag="VW561_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9944035.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSIFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            complement(7934..9955)
                     /locus_tag="VW561_001812"
     CDS             complement(7934..9955)
                     /locus_tag="VW561_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9944036.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLNYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLTSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNSGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKVGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETYAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            complement(10026..10325)
                     /locus_tag="VW561_001813"
                     /pseudo
     CDS             complement(10026..10325)
                     /locus_tag="VW561_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
BASE COUNT         3188 a         2274 c         1824 g         3118 t
ORIGIN      
        1 ataaaaagaa atagtatgct atataagaga aaaaaaatcc tataaagaaa ctagcattgt
       61 ttgcaatact tataccataa aattctctta aaaaatcaac ctcctttatc tccaaagaga
      121 agctaaaacc attactaaat gcaatcagaa aaatcaataa aaataaagtc gccgtccaaa
      181 tccccgtact aagagctgct aatttgaaac taaaactggt aaagtgctta attgatttca
      241 gacgaatgcg acactccaac ctattaaaat agttattcat caaataaaaa aagaacaata
      301 tatatgtgaa cggaaagcaa tatactccag tcgtcatatc ttgaagtaaa actaagatcc
      361 attctaatac atttggatgg attgaatatt ggcgacagcg caataaatat actgtactag
      421 ataaaacaca ggatagcagt aatataaaat aaaccaatac tgataaaaaa tcttttttgt
      481 aaaattgaac aaattgtttc attatacgta gtcctctgaa tgtagaaaaa atgtaccata
      541 aacaaccaaa caactaacaa ataaaataaa agcaagatgc ccactaacta aggaaagact
      601 gatatctttc tgatatccaa aagctaatgt tgtcacaggt tctaagtaag atagccctaa
      661 aatagcccaa aaaataccac caaccatcat ataggcaact gggatgaaaa tagctcctat
      721 ttttttcttc actagcaaag cactagctag tccaaaaata gagaacacag cgccccaaac
      781 tccataccag agagtcgtca caagactata gagcaactga ttggaatcaa ataattcttt
      841 taaggcacca ctataatctc caatataaat ttcctgataa ggagtcacta aaagattaat
      901 tcctaataat aataaatagg ggagaaaaaa gactagaaaa gaagaaataa ttgcagtact
      961 acccacaata gccagatact tctttttaga aattcgacac aattgtgctg ttagaaaatg
     1021 actctcagca tcctctatta tctgactaga atagggcagt gtacagataa gtgcagctac
     1081 taggctaatc ggtgaaaatc ctcgaataga agaagcggca aaaaaaatcg ataaaccttc
     1141 aattttataa ataccattga aagcaaggaa atttcctaaa ctcatgcaaa gaagggtcaa
     1201 aaataaagac atataaaatc gaggtgattg aacgactcgt acaagattac aaatgaaaaa
     1261 ttccatcctt actcctcctt ataataaaaa tagggtgtag cattcttttt tcatgctaca
     1321 cccacaatca accatcttta aggcttactc tgacaagtaa gttaataaga atctggactc
     1381 caagaacctg aagtatgaat tcttacatga tttccaaatt gtggcgccat agctaatcta
     1441 gtaccagaac caatataatt gtcaccacct ccattatagt acatgacaat cctagagcca
     1501 gacccaaatg aatataccgg ggtaatatct gacccactat aggcgctacg aatagaggta
     1561 tttaaccttt taccgccacc agtgctgtca ctattattaa ttccagcaga ggcgttttct
     1621 ttctcaagct cactagttgt atccgaaccc caacgcggca acgttaaatc ataatttcca
     1681 atctgtctag ctaaaactgt agggagaaac aaactagatg caattgtaac tacgcaaaaa
     1741 aggaatatat ttttcttctt ttcatgacat cgtcctctta agaagtgata aacaagatag
     1801 ttgattgatc aatttgatac atattacatt ttcattatat caataaaaga aagcgttgtc
     1861 aattatttta tttaaaatat tgtcatattt taaataaaat ggtaaaagag actcggtagc
     1921 ctcgtctctc tttatcttac tgacctaacc ttcttgccaa ttttcttggt cttctttaaa
     1981 gcgttttaga agatcaaggc cctctggcgt gatgtaacct tcttcttggg ctagatggat
     2041 aagctcgcta tagtttgaaa gcgtcacaag tttaacacca gcatctgcaa agttcttatc
     2101 tgcttttggc aattggtagc tgaaaatcgc tacaactcca agtacatctg ctccttctcg
     2161 cttggctgct gctacagctt caagaactga accacccgtt gaaataaggt cttcaaccac
     2221 taccattttt tgaccttgag ctacgcgacc ttcgatttga ttaccagctc cgtggtcttt
     2281 tggtttgcta cggatgtagg caaaaggcaa ggccatctta tcagcaataa tggctccgtg
     2341 tggaatccct gctgttgcag ttcctgcaat cacttctact tcaggaaagg cttctttgat
     2401 agcttccaca aaaccatttt caattagggt acgagtttct ggataggcta gtgtcacacg
     2461 attatcagtg taaatcggtg acttgatacc agatgcccaa gtgaagggtt cctctggttt
     2521 gaggtaaacg gcttggattt tcaagaggtg gctagcgata tctttagcaa gtgtcatggt
     2581 attctccttt tatttttcta atctagttct ttaattccag tcctgtgtcc attcatcctt
     2641 gatggcatga taagctgcaa caggatcctc agcttgggta atgggacgtc ccactacgat
     2701 atagtcactg ccgatttgat aggcatcagc aggtgtcatg actcgttttt gatctccaac
     2761 tgcaacacca gctggacgaa tccctggtgt cagacagata aaatctggat tggtagcctg
     2821 cttgatgact tgtacttcct gagccgagca aacaacacca tccaagccag cttcagctgt
     2881 cttcttggca tagtgaatca cagactcttg cagactggtt tggatatttt gaaactcctg
     2941 catctgggct tctgacgttg atgtcagctg cgttacagcg atcaatttgg cttgactccc
     3001 aagaccttca cgcgccgcct tcatcatctc tacaccacca gccgcatgga cattagtcat
     3061 atcgacacca agctgagaca agaccttcat ggctgacttg actgtattag gaatgtcatg
     3121 aagtttgaga tccaaaaaga cactatgacc caaacctttt aagtaggaca caatctcagg
     3181 ccccgctgcg taataaagct ccatccctac cttgagataa aggctttctt ctgctgggaa
     3241 aagagctaaa aattccttga ccgcctcaaa actaggaaaa tcaagagcaa tgattggacg
     3301 atgttctcgc ataggtttct ccttttacct ttactagtga gagagtctgg ccaacagaag
     3361 ccacgaaaaa aggaacctgc caacagcaag gttccacgaa aaatgggtag tctccaccct
     3421 ttcaccgtct aaccttagct gcctctctgg actgctttaa aggttttcta ttattctatt
     3481 atttctacta gcacttgtca agtaaaaaca tggtcactaa agaactataa gagaaaaggt
     3541 aaacctagcg acgcgatgag cgctgggtcg tttggtttcg attgctctct tcctcttgtt
     3601 ttttctgttc ttcttctaaa cgtttctgtt cttcttcctg ttttttctca gcttccttgg
     3661 cctcttgttt ggctttttcc tcagcctcca taattaattt atccgccaca gtgtagctgt
     3721 agattccagc ttccatgtcg accacactcg gttctgacaa ttgaggctta atcttactgt
     3781 aatatggcag tttcttactc atttcagata gaggaaccaa gacttcgtcc gaatcattca
     3841 tggtcaatcg aattaaatcg gatgtcacct tgcttggggc taattccacc ttttggatag
     3901 ccgccttgag ttctgggcta atttgagcaa gttctgagac aaaaaccttg atttgttcac
     3961 tatcattaaa gagaactgat aaataagttt ctggtaaact gttcagactc acagaactag
     4021 tctcaagctg accactggaa agaataggat aatgattttc accagaaata tagtaggcca
     4081 caatatcata ttccttgacc ttaatagtga acttagttgg aaattgatag acaagttgag
     4141 ctgattcaac ccaatagtta gacttaatct gcttttcata ttttgccttg tctagcagaa
     4201 ggttaatcgt ataatccgaa tcctgaatgc ctgaaacctg tcgaatatca tcagctgtag
     4261 tttgcaccgt tccctcaaca cgaatatctt tcatggtcgc ataaggactg agcaagtagg
     4321 cagagacaat caataaaagc agacttggaa ataaaatcgt gaaggctcgc aagatatgga
     4381 taccaggaat ctttgctttg gctggttttt cctttgtagc ctttttagca agctttttat
     4441 cctgttcctc cttctcttta gactctgatt cttctttctc ttctttctct ttgtcagcct
     4501 ctgaggatgc tactttttct tcagactctt ccttagctga ttctgaatct tcctggtctg
     4561 tttcactctc ctggtcctgt ttgtcctctg acttctcaga ttcttctccc atccgagctt
     4621 gtctttcctt ttccttctcc tcagctagag ccgcttcttc ttcagccttc ttttttagat
     4681 attcttggtt tcgtttctgc cattctgata actctttcaa ttcttcgagg atttctttgt
     4741 cctcattttt cttatctttt gacatttact ttccttatga taaatctttt ttcaacaatt
     4801 gataaaaatc tgctagagat ttcaattcct tagaagcttt catcttagct tggtaatctt
     4861 ccttgtgact tagtaagtga gaaagcttct cttccaaact atccaaggtc aaatcgcttt
     4921 cttgaaggtc ttctgcatag cctttcttaa caaagtaagc tgcattttca atctggtcac
     4981 cacgactagc ttcacgacca agtggcacaa tgacatgcaa ttttgctatc gccaagagct
     5041 caaaaatcgt attggcacca ccacgtgtca caacaatatc agccaattcc atcaagggtt
     5101 gatagagatc ggtcacatag tcaacacgaa aaagattttg gctcaactca ttcaggctag
     5161 aatctccagt tagattgata atattgtagc gctctgttag ttctttctta tggtctgtca
     5221 ccaattggtt aaagacacga gcacctgcag aaccaccaac aaacaataca gtcggcaatt
     5281 tatgattaaa gtggctttga atatccacca attcatctgg ttctggattt ttttgatctg
     5341 aaaccttggt cactgctccc acatgctcaa ccttagacaa actcgaagct tgctcaaagg
     5401 ttgaatacat cttagtcgca aacttatagg cgattttatt ggccaagccc atagacaggt
     5461 cagattcgtg aataaagaca ggcactcctg acacacgcgc agcgataaca ggcggtactg
     5521 agacaaagcc cccctttgaa aaaagggtct gtggacgaag tcgcaacatg ataaagagcg
     5581 attggacgat tccccagcca actttgaaga cgtccagcat attttgccaa gagaaatagc
     5641 gacgcaattt tccagtcgca atagaatgga aggtgacatc caaacctgac ttaaggattt
     5701 cttggtgttc gataccacgc ttgtccccga tatagtggac ttcccaacca tcttcgatga
     5761 acttgggcat taacaaaaga ttgagggtaa cgtgtccaac cgtcccccca cctgtaaaga
     5821 caattttttt catattattc ttttaactcc gctactgtgt cgataaagag gtcgccacgt
     5881 acttcaaagt tagcatacat atcccagctg gcattggcag gactaagaag aaccacatct
     5941 ccttgagtcg caagctcata ggccttgcgg gtcgcatctg caatatctgt cgcctccaca
     6001 taagcgacac cagccttgtc tgctgcccgt ttgacacgtt ctgcagattg acccaggatg
     6061 accatcttct tgagtccagt aatgtctggc accaattcgt caaactcatt gccacggtcc
     6121 aaaccacctg caatcaagac gaccttgctg ttgtcaaatc ctgataaggc tttttgagta
     6181 gccaagatat tagttgattt actgtcgtta tagaatttaa cacccttgat gtcatccaca
     6241 aactggagac ggtgtttgac accaccgaag gctgaaagag tttccttgat ggtttgattg
     6301 tccacatcac gaagcttggc tacagcaata gtcgcaaggg cattttccac attgtggcta
     6361 cctggaacac cgatttcatt cgctgccatg actacttcac cacggaagta gagttgacca
     6421 tcttccagat aagctccatc aaccttttca agtgttgaaa atggtacaac agtggcttct
     6481 gtcttggaag ccaagtcttt tgccaagtct tgattaaagt tcaagacaag gaaatcagct
     6541 gctgtcatct tgttctggat attccacttg gctgctacat attcctcaaa taatccatga
     6601 taatcgatat gagttggcat aaggttggta ataaccgcaa tctcaggatg gaattcttga
     6661 acacccatga gttggaaaga agaaagttcc ataacaagcg tgttcttgtc tgtcgctatt
     6721 tgagcaacct gactggcagg atagccgata ttccctgata aaagaccatg ttggccagca
     6781 gcagtcaaaa cttccccaat catagtcgtt gtggttgtct taccgttcga tcctgtgata
     6841 ccaataatcg gtgcttctga aatcaaataa gccaattcca cctcagtcaa gactggaatt
     6901 cccttggcca aagccttttc aatcatggga ttgctgtagg ggatacctgg attttttacc
     6961 atgagggcga actcttcatc caagagttcc aaaggatggc cacctgtaat gaccttgatc
     7021 ccttcttcca gcaaacattg cgcagctgga ttgtcctcga atggtttccc atcatttact
     7081 gtcacaatgg cacctaactt gtccaacaaa cgagccgcag attcaccaga cttggccaaa
     7141 cctaaaacaa gaactttctt atttttaaat tgatctatta ctttcatgtc tcgaactcca
     7201 tttctactcc tactatttta ccatttttat ggaaataaaa aagccacaaa gtgtgtttgt
     7261 gactctttct tctagctgaa tcttaccata tcatctatgt gataaatcgg taactcgaat
     7321 gacctgatcc acttgctccc aaatcagagg attgtgggtc gcaataataa tggtccgatt
     7381 cggatttttt aaagattcta ggatggaaag taattcctca gagtttttgg ggtctaagga
     7441 agcggttggt tcatctgcga ggatcaaagg tggatccttt aaaattatct tcgctaatgc
     7501 aacacgttgt gcttctcctc ctgataactc aaatataggt tgcttcaaat ccaaataaga
     7561 gaggtttaca cggtttagag cttgtttcat caaagagatt ttctcttttt ccttcaactt
     7621 tttaccaact aaacccagat tgagattctc tttgacggtt tggctttcaa ttaagccaaa
     7681 atcttgaaat aagtatccca agtaatctct aaagaaaata gaaggcttga tgtccttaag
     7741 agaagtgcca tcatagatga tttgcccttt gtcatatggc tctaatcgtc caatcatatt
     7801 caagagtgtt gtcttaccac agccacttgt accgattaag gcataaattt tcccaccttc
     7861 aaaatgaaga tttgtatctg aaaatagctg acggcttcca aattttttag atatattctt
     7921 tagttcaatc atcctatttt cctttcataa ttgtcataga aacacgagat tctttctgcg
     7981 cttgacggta aagcgtcaga actgcactag ctagaaagac taataaagtg agcaagccaa
     8041 tcaccaagtc tcgactgctt aaaataaaga gactagcacc aaatacaaaa ctggcaaatt
     8101 gactaaccat atactgagca tatgtttcaa aaaatcgtaa acctgaaatt cgtttaatca
     8161 agatatctcg gcggaattgc tcgaaatata gaagattgac agaataaaat agtaacaagg
     8221 aactagctat ccccacaata gctcctaaga ttaaagttgc tgtttcagtt tgaacttcat
     8281 tataacgagt taaataaaca cttcttcctt ctttaagata ggatacttgc tcataaattc
     8341 caactttctt caagagttct agcccactct catatccttt gataaagagt tgttttccag
     8401 cattgataga ccaactagat aaggatataa aactatcacc tgtagaagtc ggcgtgaata
     8461 ccactaaaat cggatcagtc aaatactgag tagatacggg attctcaccg ctattataaa
     8521 caaaccgctt ttctcccatt gaaagataac taacgtgcgc tttcatctca taatccaaag
     8581 gagcacttgc ctcctcacca gattttccat aataactcaa tctttcttca aaaactttct
     8641 taagttctgc ttctcgagag cgcaaatgtt ctgggagcaa gaggataaac tcaccttttt
     8701 ggagatgggc taacttctgt ttggtctcag catctaccac gaccttttcc ttgtccaaat
     8761 aactgggact aacatagagc gtattagcat ctggactata ggtatccagt gtctctccct
     8821 gttcattttt tccttgtgga ttggcaaaat ggagcagatt atcctttaca taaagagctt
     8881 gttcttcttc gattgcttcc ttggcaaagg cataccactt gctctgattt tctgtatctt
     8941 ttcctctatc acctaagcca aaagaaattt gataatagtc tgctctatcc tgccatgctt
     9001 gttttgaaat ttcaagttct ttcaatcgtt ggtaagacgt caaacctgtc ttaacagcgt
     9061 atcctactgt aaagacagca accaactgac acaatagggt taaaatcatc aggcgtttaa
     9121 ggggtaatct ccccttaata acgggaacta atgctttgta attcaaactc attagataaa
     9181 gaagcattag taaaattgaa atcgccaata aaaacaacag atagaaacta atcccaaaac
     9241 cataggtggc taacaagata ggataaaaca aaccttgact aaaaagaacg actcccccac
     9301 ctaggaagga aaggagggct gatagaagga gccatttgat atcagtagat aaagaatgcc
     9361 ccatgatgga taagagagtc tgaccagaaa agagttttat acctgctgct ctcatttcct
     9421 taatccgagt gataatcact aaagcaaaga aagataaacc aaatattgct aaactaatta
     9481 aaataagggg atttagtaat attcgaaaag caagaaaata gggcggtatc tttcggtcag
     9541 cacttgcttt ataacccaaa tctcctaatt tatcggcaag cttttctttc gtcaaggagc
     9601 ctgacaaaag gagataacta tttagcggat cactacgttc acgactttct tggctagctt
     9661 cttggaattc ttttggtaaa gttccctgac cataagttgc ataagtaaag tgagtcgtcc
     9721 catccttact cggctctaca attcttctag ctattaaact ctgttctgag tttgcaaaat
     9781 tctccaattc ctgttcaaat acctcacgcg tcggttcctg agtatctttt ttgacacgaa
     9841 gtaaagaaac ggaatcatag cttgcatata aatattgtgg cgcacgtaag acaataatcc
     9901 aagcaaggaa gaagctgaga aaaaaagttg ataataatat gaatagtttc ttcatagtag
     9961 actccttgta aacaaaattc cccctgtaat ttcttacaag gggaacgatt taaatcaatg
    10021 aacgattagt cataatcaca gtaaaatgct acttgttctc cccatttagt ccaaatccat
    10081 gcataggagg tttttccagc aggagcttct cctttatcag aatgaccatc ccatctgctc
    10141 acgatggatg aataatgata ttttttacca tgatagtaat ttgaaaaagc ctaaccacct
    10201 cctgaacctt ctccatatgt ccatactcct ccatctggat attgtacagc agctgatgca
    10261 gctcccaata atgtaaaatt tgaaataaga gctagagcaa gtaatctatg ttttttcgtt
    10321 ttcattttat tttttctttc aaaaaaagca caccttgagc aacaatgcaa caaaataaat
    10381 cctcctctat cttttattga aatc
//
LOCUS       DAVKQL010000069        10356 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVKQL010000069 DAVKQL010000000
VERSION     DAVKQL010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10356
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.069"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(346..738)
                     /gene="rpsI"
                     /locus_tag="VW561_001814"
     CDS             complement(346..738)
                     /gene="rpsI"
                     /locus_tag="VW561_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU9944037.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(757..1203)
                     /gene="rplM"
                     /locus_tag="VW561_001815"
     CDS             complement(757..1203)
                     /gene="rplM"
                     /locus_tag="VW561_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU9944038.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            1559..1876
                     /locus_tag="VW561_001816"
     CDS             1559..1876
                     /locus_tag="VW561_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEU9944039.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            complement(1921..2733)
                     /gene="folK"
                     /locus_tag="VW561_001817"
     CDS             complement(1921..2733)
                     /gene="folK"
                     /locus_tag="VW561_001817"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEU9944040.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIHILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(2776..3330)
                     /gene="folE"
                     /locus_tag="VW561_001818"
     CDS             complement(2776..3330)
                     /gene="folE"
                     /locus_tag="VW561_001818"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU9944041.1"
                     /translation="MDTQKIEAAVKMIIEAVGEDANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            complement(3311..4633)
                     /locus_tag="VW561_001819"
     CDS             complement(3311..4633)
                     /locus_tag="VW561_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU9944042.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFNSPYLIHYTDQISINGESISEARLEALMADY
                     QSLMGGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLESIAEQKAGIIKQGMPLVTGRIAPEALAVIDRIAEEKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNVTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            complement(4635..5609)
                     /gene="folP"
                     /locus_tag="VW561_001820"
     CDS             complement(4635..5609)
                     /gene="folP"
                     /locus_tag="VW561_001820"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU9944043.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGADLVNDITGLMGDEKMAHVVAEARAQVVIMFNPVMARPQHPSSLI
                     FPHFGFGQAFTEEELADFETLPIEELMEAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEENGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            complement(5704..6411)
                     /locus_tag="VW561_001821"
     CDS             complement(5704..6411)
                     /locus_tag="VW561_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9944044.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVTGISIVVLVLFIIGARKTKLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            complement(6464..7882)
                     /locus_tag="VW561_001822"
     CDS             complement(6464..7882)
                     /locus_tag="VW561_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEU9944045.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTVMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            8072..8884
                     /locus_tag="VW561_001823"
     CDS             8072..8884
                     /locus_tag="VW561_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9944046.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKRTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKEMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            9223..10242
                     /gene="adhP"
                     /locus_tag="VW561_001824"
     CDS             9223..10242
                     /gene="adhP"
                     /locus_tag="VW561_001824"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEU9944047.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSETMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
BASE COUNT         3069 a         2348 c         1993 g         2946 t
ORIGIN      
        1 agtagattga aactagaata gtacacctct acttctaaaa cattgttaga attcgatttg
       61 actatcctca gtttgtccta ttcttatttc accttactat aaaaataagg taaacttttc
      121 aaaaaaacac gaaaaaagca cccataaggt aaacttttga gtgctttgaa acgttgatat
      181 aatcgtattg attaacgttt tgaggcaact gaccttgtca ggttgcagcc acatttgtaa
      241 gcgactaaag tcgcaacaac tgtgtcaatt gcaccaattt agtaagaaag tataaaaaag
      301 aacaccccga aaggtgctct gtataagtat agtaattctt tcgaattaac gtttactaaa
      361 ttgtgatgct ttacgagctt tcttaagacc tggtttctta cgttcaactt tacgtgagtc
      421 acgtgtaaga agtcctgcgc gtttcaatga atcgcggaag tctgggtcta cttgaagaag
      481 ggcacgagcg ataccgtgac ggatagctcc tgattgacca gcgtatccac cacctacaac
      541 gttaacgaaa acgtcgtatg aacctacagt tgaagtaact gcgaatggtt ggttgatgac
      601 aagacgaagg tcagcgtgtg ggatgtactc ttcaacatct tttttgttaa cagtgatttt
      661 accagttcct ggaacaaggc gaacgcgtgc aacagcgttt ttacgacgtc cagtacctgc
      721 atattgtgct tgtgacatac tttattgttc ctttccttag ataagtcctg aaatgtcaag
      781 aacttctggt tgttgtgcag cgtgagtgtg ctcagctcca acaaatactt tcaacttcat
      841 accttgagcg cgtccaagtg tattgtgtgg aagcatacct ttaactgatt tctcaatcaa
      901 acgtactgca tttttagaac gaagttcacc tgcagagatt tgtttcaatc cacctgggtg
      961 gtttgagtga gtgtagtaga ttttatcagt tgctttttta ccagtcaatt taactttttc
     1021 agcattgata acaatcacga agtcacctgt atcagtgtgt ggtgtaaatg ttggtttgtt
     1081 ttttccgcga agtacgctag caactactgc agaaagacgt ccaagtggta catcagttgc
     1141 gtcaactacg taccatttac gttcaacttg gcctggttta gccataaatg ttgttttgtt
     1201 catgatttct cctatatata gttcgatttt tgtttacggt gatgggtgtt ccttcccatc
     1261 gaaaagtatt tggaaggttc cggggccttt caaatggggt aaacaatacc gcctactatc
     1321 ataccaaatt ttacctctaa aagtcaatag atatgaaaaa tatttttcgg taacttctca
     1381 aagtaactga aagttatata atcaagttaa tttgggaatt tacctattaa cttcggtaga
     1441 tttttaggaa ttttttccct atttctccct gtaactaaaa tccttttcaa aaaatcacct
     1501 tttcttgatt tttaatccta tttgctgtat gataagggaa aagaaagggg acagagatat
     1561 ggcttttacc aatacccaca tgcgatctgc tagttttggc attgttacca gcttgcctga
     1621 tgacatcatt gactcttttt ggtatatcat cgaccatttc ttaaaaaatg tctttgaatt
     1681 ggaagaagaa ctcgagtttc aattgcttaa taaccaagga aagattacct tccacttttc
     1741 aagtcaacac ctccctacag ccattgattt tgactttaac catcctttcg accctcgtta
     1801 tcccccaaga gtactggttt tagacatgga cggtagagaa actatcctcc tcccagaaga
     1861 aaatgaccta ttttaaaaac tctagccttc agttgcaagt aactgaaaac tagagttttt
     1921 ctattttttc aaagcatcat acaagttgcg gatcggttgt tttaatatcg gatggataaa
     1981 atgaggcgca atttcctgta aggactcaag gacaaaaagg cgttccgcta tgtaaggatg
     2041 aggcaatatg aggtcgtctg tataaaggat ctggtcctcc acaaagagca agtccaaatc
     2101 aatcaaacga ggtccccaat gcacttctct cacccgtccc agctctgact caatggctaa
     2161 caaggtttct aacaagtctt gtgctggtag ccaggtttcc acctcaacca cttgattggc
     2221 aaagctatcc tgttccactc caccccaagg ctccgtcgct aagacactgg actctttgag
     2281 aatatggatg ccacgagctc gcagtttgtc aatggcttgc ttcaagtttg cttgcttatc
     2341 tcccatattg cttcctaggg cgataaaggc tcgttgcttg cggcgatgaa tggttaccga
     2401 gcaagtatct agtgacaaat gcaccggtgc ccaaggtttt ttcagttcca acttcatttc
     2461 ttggacaaga ggataagact caaaggtacg ttccaccagt ttataggcta ccgtttcaat
     2521 caaatcttca ctcgtttcct gaaaccaagt cgtccactgc tgacacaatt ctccgtaatg
     2581 gacagaggct gttaaatcca agtctgtagc tgccttggtc atatcatagg ataggatggc
     2641 tgaaacgaca aatttctgcc ccaattcttt ctcactagga aaaagaccat gataggcaaa
     2701 catttccaaa tccttaatct gcagttgatc cataatgatt cctttctaga aaaatccgct
     2761 tgaagcggat tctttttata gccccattaa acgataagct tggtcgcgga ggtccttatc
     2821 tgtttcaaat agaccacgag ctactgtcgt caaggttgca gtgcctggtt ttctaacacc
     2881 acgcatactc atacacatat gttccgcctc aatgacaaca aaggctcctt tagcacctag
     2941 atagtccatc aaggcatcgg ccacttcgat attcaaacgt tcttgaattt gtggtttttt
     3001 cgaataaact tcaaccgtac gggctagctt agacaagcct gccacacgac catctggaat
     3061 gtaggcaatg tgcgctctac cataaaatgg caagaagtgg tgttcacaca tggtatggaa
     3121 aaagatatcc ttttctacca ccatattatc gtcaataatt tcaaaggatt ttgacaaatg
     3181 ttcctctgct gtttgaccaa gacctgaaaa aatctcttga tacatacggg ctacacgagc
     3241 aggtgtttcc tgcaaaccct cgcgattagc gtcctctcct acagcctcga taatcatttt
     3301 tacagctgct tcaatctttt gtgtatccat tctcgttctt cctctccatc agataggccc
     3361 tcacttggct caagaaatac aaggaacctg tgacaatcct aactgtttgt ttctcttctt
     3421 ttttatctgt cacattctgc tctagaaaat catgccaatc ttggtagctg agatttctag
     3481 acttagctgc ctctttcagc acgctttcat ccgttgcccg actatccgca aaatgtgtta
     3541 gagtaagctc ggtatctggc atggctccca gcaagtccaa catatcctcc aaggccttgg
     3601 ttttgataca agtgaagagg atttccttat gataatccgc aaaacgttct tgcaaggtta
     3661 ccaacaaagc cttgatagca tggggattat gggctccatc caaaatcatc aagggatctc
     3721 ttgacacgat ttccaaacgc cctggccaac ttgtttcttc caaggcttga ccaagaaagt
     3781 cattgcttgc tagctctcga ccatcttctt gacaaaaagt atcaagtaaa gctatggcca
     3841 tcccagcatt ctctatttgg tacaaaccaa gcaggctagt ctggaagcga ccttgtctga
     3901 cagcacttgt atagtcaaag acttcccctg tcaccacact ttcttgatga cgaacctgat
     3961 aatctgtccc gtaggcaagt ctcggcgcat ctttctcttc cgcaatgcgg tcaatcacag
     4021 ctaaggcttc tggagcaata cgccctgtta ccaagggcat cccttgtttg ataatacctg
     4081 ccttctgctc tgctatggac tccaaggtgt caccaagtag ggccacatga tccaagccaa
     4141 tagttgtaat tcctgtcaaa atgggctgac agacattggt actatccaaa agtccaccca
     4201 tgccaacttc catgatggcc acatctactt gctctgaggc aaagtagtca taggccaggg
     4261 ctgtgataat ctcaaactcg gttgtgccct gtaaattggc gaccgcttct ccccccatca
     4321 aagactgata gtctgccatg agagcttcta gcctcgcttc tgagatcgat tccccattga
     4381 tgctaatctg gtctgtgtaa tgaatgagat agggcgagtt aaacacgcca actctcagcc
     4441 ctagcttttc tagcatcttt ttcaaaaaag caatagtcga gcccttgccg ttagtccctc
     4501 cgatatggag gaccttgagt ttgagatggg gattgccacg caaagctaac agttccacca
     4561 ttcgttccaa accaaaatgc ggttgatccg tccggtagtt agcaatccac tgattgtttt
     4621 caatttcttt catcttattt atattgtttt aaatctaaat tttccgcttc atcagccaga
     4681 cgaatggcag aggcaatttc aactgccatc ctgtgactag ctacgtcatg cacgcgcacc
     4741 acttctacac cctgtctcgc agcgatacta gttacatgag ccgaagccgt gtcccgattt
     4801 cggaaaccaa gctctgtctc aggattgact tcaaaaccat tctcctctag gatattgatg
     4861 acaaatcgct tgcgcgacac tccgagaaag attggatagc ccttctgatg tagtttatcc
     4921 aggtcccgta aaagaagcag attttctttc ttggtcagac caaagccaat tcctggatcc
     4981 aacaggatat tttctggtgc aataccagct tctgccgctc tcgctagtgc tcgttcaaag
     5041 aaagcctcca tcaattcttc gattggcaat gtttcaaagt cagctaactc ttcctctgta
     5101 aaagcttgac caaaaccaaa atgagggaag atgagcgagc tagggtgctg aggtcgcgcc
     5161 ataactggat taaacatgat gaccacttgc gctctcgctt cagctaccac atgagccatt
     5221 ttctcatcac ccataagacc agtgatatca ttgactagat cggcacccgc agccaaagca
     5281 gcctctgcta cttgactctt ccaagtatca atagagatga ggacatcact ttccttgcga
     5341 atcgctttga tcactggaac aacacgctgg atttcctctt ctatctcaac atagctactt
     5401 cccggccgag tcgattctcc gccgatatct agcatactgg ctccttctgc tatcaattta
     5461 cgagcctgct ggagcgcctg ctcaagagca aaaaattgac caccgtccga aaaggagtct
     5521 ggggttacat tgataattcc gcaaataact gtctttgcat gattggcttt acttgacata
     5581 tcggtcactc cctcaaggat tttcatcata ttatttttct attttaccat aaaaagaaaa
     5641 agatggacac gattgcattc atctttccct agtagaaaga ggtcggttcc tacataaaaa
     5701 cttttaaaca gaaattccta atgtccgact cataatcacc acaagagcca acaaacagaa
     5761 agcaatccca ttaacaatca tgtgaagcaa gatcgacatt tccaaacgtt gggtcttgta
     5821 ggccgtccaa gatagaactg tcgacatacc tccataaatc aataaagaag gtaaattact
     5881 tggttgatgc aataaagcaa acacaatcgt accgactaca aatcccaagt tctccttgcc
     5941 tcggaaaatc tttttaggaa caatcccacg gcacaagatt tcctcacaaa tcggagcaag
     6001 caaggctagc aagaagaaac tggaaatcaa cgaactattt tgaaccatat cgttaatctg
     6061 agactggtta gctgttgtcg tctcatttga cagttgcaac aagatagaac caagtatatt
     6121 tgacccgaca ataactaaat aactcaatcc caaacgtgcc aaatctttag ctctaaaaaa
     6181 agaaaaatta aaactagcta acttggtttt acgagctcca ataataaata gcaccagaac
     6241 cacaatagaa ataccagtaa ctatcagccc tgactgtagc agggctactt cttttaaagt
     6301 caaaatagag gtaaccacta ggggaacctg atataaaagg acggcaagta aaaagaccaa
     6361 aatccagcct gcacgattca acaattcttt ccacatattt ttctctttca tcacctatct
     6421 cctttgtatt aaaaaagggg ggaaatcccc ctgggtcatt ctattataag gccatgctga
     6481 tgtagttaag gataaacaag gcatccaaaa tccaaatcat ggcatgaaca tctttaactt
     6541 gacctttgac aagcttagtc aaagtgtaag tcaagaaacc aactgcaatc ccttgagtga
     6601 tagagtagct gaatcccata aagatagatg tgaagaaggc aggaactgct tcagacatat
     6661 catcccaatg gatatttttc aagctaccaa gcatcataat cccaacgata atcaagattg
     6721 gagctgtagc cgctgttggt acgatcgcta gaagtgggct aaagaagctt gaaatcgcaa
     6781 aacagatagc tacaaccaag gctgtcaaac cagtacgtcc acctgcaccg ataccagcag
     6841 cagactcaac ataagtcgtt acgtttgaag tacctgcaat ggcaccgact gtcgttccaa
     6901 tcaaatcaga gtaaagagcc ttatccaatt tggctgattg gtgattttca ccatttgtcg
     6961 ctacaatacc aactttttca cctgtaccga tcaaggtacc aattgtgtca aaaatatctg
     7021 tcaatgagaa ggcaagaatg gccatcagag tttcaggcaa gcgagctgta tctgaaacca
     7081 aagctcccaa tccttctgaa ccaagagctg caccaaagat tgtcttcaaa tcttcaaaag
     7141 ctgcaccaac atggttatta gcaaaatcga tactagacaa atcaaccaaa ccaactgcaa
     7201 tagcaagaac agttgttgtc aagatagaga gaataattcc ccctttaatc cctttgatga
     7261 caaagaagat agtaatggca agtcctgcaa gagccactaa aacagctgga ttattaaagc
     7321 tgaccaatcc tggaactgct gaagagtttg ctgcaatcgt tgcttgagct ttgtcagccc
     7381 cttctcctac aacagtatag ttgcctggat caatcgtgaa tttcaaaagt ccagcattct
     7441 taatccctac ataggcaagg aagacaccga taccagctga aatagctgag cgaagagcat
     7501 tgggaatcga ttcaatgatc attttacgaa catttgtcaa ggtaataatc aatgaaataa
     7561 tcccacagat gaagaccata gctagggctt cttgccaaga ataaccaagc ccgaatacaa
     7621 ctgtaaaggt aaagaaggca ttgagtccca tacctggcgc ttgggcataa ggtaagttag
     7681 cataaaaagc catcatcacg gtacccgcta ctgcaccaat aatcgtcgct aggaagacgc
     7741 cctgagcagg cattcctgtt tgtgaaagta tttgtgggtt tacaaagaga atatagctca
     7801 ttgcaaagaa agttgttaaa ccagcgagaa cctctgtacg aacgtctgta ccgttctctt
     7861 ttagtttaaa taatttgtcc atcatcaatc tccttttaat ttttacgaac aataatagaa
     7921 ttatatcatt tatcattcac tttttcaatg tctttgtttg atttgtttaa gaaattaatc
     7981 caatttcatt ttttgtttgg aatctgcttt tctgcctgct ttctgttata atagaagaca
     8041 tcttatctga aaacacacta gaaaggtcca tatgacgaaa aaaattattg cagttgacct
     8101 ggatggaacc ctgctcaact cagacagtca aatttctgac tttaccaaaa gaaccatcaa
     8161 aaaagttgct gaaaaaggcc atcaggttat tattacgaca ggtcgccctt accgtatgtc
     8221 aaaagatttt taccgtgaac tgggcttaga cactcctatg attaacttca acggatccct
     8281 tactcattta ccagaccaag tttgggattt tgaaaagtgt ttgactgtag acaaaaaata
     8341 tctgctagat atggttcaac gttcagagga cattcaagcc gattttatcg ctggagaata
     8401 tcgtaaaaaa ttctacatta caaatcccaa tgaagaaatt gccaatccca aactatttgg
     8461 tgtagaagct ttccagcctg aagatcaatt ccagcctgaa ttggtgacca aggaccctaa
     8521 ctgtatcctc ttgcagacca gagccagtga caaatatgcc ttggcaaaag aaatgaacgc
     8581 cttctaccag catcaacttt ctatcaatac ctggggaggt ccgctcaata tccttgaatg
     8641 taccccaaaa ggtgtcaaca aggcctttgc tttggactac ttgctcaaga taatgaatcg
     8701 tgacaaaaaa gatttgattg cctttggaga tgaacacaat gataccgaaa tgctcgcttt
     8761 tgctgggaag ggttatgcca tgaaaaatgc caatccagag ctactccctt atgcagatga
     8821 gcaaatttcc cttaccaacg accaagatgg ggttgccaaa accctacaag acttattctt
     8881 ataacctata ctgatactca atgagaatca aagagcaaac taggaagcta gccgtaggtt
     8941 gctcaaagca ctgctttgag gttgtagatg aagctgacgt ggtttgaaga gattttcgaa
     9001 gagtatgacc agcttgcaag ctggtctttg tgctcttttc acagtctcat caaccagtta
     9061 atcgattgtt ctactttttg tctccaaaac cttggaaaag gctttctttt gtgatatact
     9121 tcacatataa atacaagaga gctcgtcaca ctcgactctg ttgacaaaaa atcaatccag
     9181 tcccgttgtc cctgttctcg gtcaatatca aggaggatag ctatgaaagc tgttgttgta
     9241 aatccagaaa gcactggtgt tgctattgaa gaaaaagtac tccgtccact tgaaactggg
     9301 gaagcacttg tagaagttga atactgtggt gtttgccaca ccgacctcca cgttgctcat
     9361 ggtgactttg gtcaagtccc aggacgtgtt cttgggcacg aaggtatcgg tatcgttaaa
     9421 gagattgctc cagatgtgaa aagccttaaa gtcggtgacc gcgtcagcgt tgcttggttc
     9481 tttgaaggat gtggcacttg cgaatactgt acaactggtc gcgaaaccct ttgccgtaca
     9541 gtgaaaaatg ctggctactc agtagacggt ggtatggctg aacaatgtat cgtaactgct
     9601 gactatgctg tcaaagttcc tgacggactt gatccagccc aagcttcttc tatcacatgt
     9661 gctggagtaa caacctataa agctatcaaa gaagcaaaag ttgaaccagg ccaatgggtt
     9721 gttctttacg gtgctggtgg acttggtaac ctcgctgttc aatacgctaa aaaagtattc
     9781 aatgctcatg ttatcgcagt cgatatcaac aatgacaaac ttgcccttgc aaaagaagta
     9841 ggcgctgaca ttgtgattaa cggcctcgaa gttgaagatg tagctggact cattaaagaa
     9901 aaaactgatg gaggagctca ttcagctgtc gtaactgctg tgtctaaagt tgccttcaac
     9961 caggctgttg actccattcg tgctggtggt cgcgtcgtcg ctgttggtct tccttctgaa
    10021 acgatggaac tcagcatcgt taaaacagtc ctcgatggaa tccaagtcat cggttctctt
    10081 gtcggaactc gtaaagactt agaagaagcc ttccaatttg gtgcagaagg tctggtagtc
    10141 ccagttgttc aaaaacgtcc agtagaagat gctgttgcca ttttcgacga aatggaaaaa
    10201 ggccaaatcc aaggacgtat ggtactcgac ttcacccact aatctaatag aaacctattt
    10261 taaaagttgg aagcttcttc caactttttt atattaaatt ttaaataaga attcaagatt
    10321 actcctcaaa aactcaacat atcaacatac aaaaat
//
LOCUS       DAVKQL010000070        10308 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVKQL010000070 DAVKQL010000000
VERSION     DAVKQL010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10308
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.070"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            103..272
                     /locus_tag="VW561_001825"
                     /pseudo
     CDS             103..272
                     /locus_tag="VW561_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            472..3747
                     /gene="rexB"
                     /locus_tag="VW561_001826"
     CDS             472..3747
                     /gene="rexB"
                     /locus_tag="VW561_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEU9944048.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPVKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRTDLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWNVFLKEGVVTKQLQDLTATMEAIEQERQAEVWKAFCHVL
                     EQFATVFAGSQISLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKIAQNTSLLTDEERQNINQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLVLSAPSLFNESESKESAYLQELVHLGFSRKEKRMNHKRLSKEDMGSYHSLLSSL
                     VAYHQQGETSDTEQDLTFVKVLARVMGKKLDQQGLENPALPTSPSSKQLTKDTLQALY
                     PAEQEFYLSTSGLTEFYRNQYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PDEDSFDNRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPVIE
                     TIKEEANFGGKEQAFIQLDNGRSVFVRGKVDRIDRLKADGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSHLGEFYNKNKANQLTDEEFQLLLDYNAHLYKKAAEKILEGQFAINPYT
                     ENGRSIAPCVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWLEKIREE
                     LNR"
     gene            3744..7394
                     /gene="addA"
                     /locus_tag="VW561_001827"
     CDS             3744..7394
                     /gene="addA"
                     /locus_tag="VW561_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEU9944049.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVELKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKETFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SDSFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQESDSQAYQAVLAHVVAISKEKNGRALTNASRKADLKPLADTYNEERKTQ
                     FAKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNKHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVVVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLESKEYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTALGQEIFANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            7564..8742
                     /locus_tag="VW561_001828"
     CDS             7564..8742
                     /locus_tag="VW561_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944050.1"
                     /translation="MTLPVRKSLHDAVLQASEADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNVIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
     gene            8951..>10308
                     /locus_tag="VW561_001829"
     CDS             8951..>10308
                     /locus_tag="VW561_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9944051.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGIGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPSPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAVVSDKGEPEVQPTLPEAVVTDKG
                     ET"
BASE COUNT         3282 a         2076 c         2176 g         2774 t
ORIGIN      
        1 gctcacaata agagagaact tttttacgaa aatctattga atatgccata aaaagattat
       61 accacattgt gtactatttt tggttcattt tactatagtt caatcattct atcaagaata
      121 ttgaatatat aaaggcacgt aatattaaaa cttcttctga tatcgaatta gagtccgatg
      181 ttgatggcga taaatcagac aatctacccg tcgacattaa gatactggga caacacattg
      241 aagtgtattc cgtacctaag aaacaatcat aaaactcaaa gccttgttac tgaggctttt
      301 ttattatgtg attttcagta gccagactta ttctatctaa tacaagtaaa gctaagtatt
      361 taagatttag cagataatga agaaaagaaa ggatggccta aatattttaa cagatttacg
      421 aggctaatca tggtataata tagggtgatg aaaaattcca acgaggctga gatgaaatta
      481 ctttatactg atattcgaac ttctttgaca gaaattctaa caagagaggc agaagagcta
      541 gttgcagctg gcaagcgggt cttctacatt gcccccaact ctctttcttt tgaaaaggaa
      601 cgcgccgtgc tggaatgctt gtcccagcag gcttcttttt cgattaccgt cacgcgcttt
      661 gctcaaatgg ctcgttatct ggtcctgaat gatttaccag ttaaaactac tcttgatgat
      721 atcggtcttg ggttggcctt ttacaaatgc cttgccgaac tcgatcccaa ggacttgcgt
      781 gtttatggcg ctattaagca ggatcctcaa ttgatccagc agttaattga gctttaccat
      841 gagatgacca aatctcagat gagttttttg gacttggaga atttaacaga tgaggataag
      901 aggacggatt tactcttgat ttttgagaaa gtaacagcct atcttaatca aggtcagtta
      961 gcccagggaa gtcagttgtc ccatttgatt gaggctattg agaatgacaa ggtaagtagt
     1021 gattttaatc aaatcacctt ggtcattgac ggctttactc gtttttctgc tgaggaagag
     1081 cgggttgtgg acttacttca cggcaaaggt gttgagattg ttatcggggc ttatgctagt
     1141 aagaaagcct ataccagtcc ttttagcgag ggcaatctct accaagccag cgtaaaattt
     1201 ctccatcatc tggcttctaa ataccaaacg cctgctcagg actgttctca aactcatgag
     1261 aagatggata gttttgacaa ggcctctcgt ttgttggagt cttcttatga cttttcagaa
     1321 ctcgctttgg atgtcgatga gaaagaccgt gaaaatttac aaatctggtc ttgtttgacg
     1381 caaaaggagg agttggagct agtagcccgt agcattcgtc agaaattaca tgagaactca
     1441 gacctgagct acaagcattt tcgtattctc ttgggggatg tagcttctta ccagttatct
     1501 ctcaaaacca tttttgacca gtatcagatt cctttttatc ttggtagaag cgaagccatg
     1561 gctcatcatc ccttgactca gtttgtcgag tctattttag ctttaaaacg ttaccgtttt
     1621 cgtcaggagg atttgattaa tcttcttaga actgatttgt atactgacct cagtcagtct
     1681 gatattgatg cttttgagca atatatccgc tatcttggta tcaatggctt gccagccttt
     1741 cagcaaacct tcaccaaatc ccaccatgga aaatttaatc ttgagcgttt gaatgtcctc
     1801 cgtctgagaa ttttagcacc tcttgaaacc ctctttgcta gccgaaaaca aaaggccgaa
     1861 aatctcttgc aaaagtggaa tgtctttcta aaagaaggag tggtgaccaa gcagttacaa
     1921 gatttgacag ccactatgga agctatagaa caggaaagac aagccgaggt ttggaaggca
     1981 ttctgtcatg ttttagaaca atttgcgact gtttttgctg gttcacagat tagtctagag
     2041 gacttcctag ccttgctcca ttctggaatg agtttgtctc agtatcgcac tattccagcg
     2101 acagtggaca ccgttctggt gcagagttac gatttgattg cgcctttgac tgctgacttt
     2161 gtctacgcca ttgggctaac tcaggacaat ttaccaaaaa tcgcgcaaaa taccagtctt
     2221 ttgacagatg aagaaagaca aaacataaac caagcgaccg aagaaggcgt tcaattactg
     2281 attgccagca gtgaaaatct caagaaaaat cgctacacta tgctttcttt ggtcaattct
     2341 gcccgtaagc aattagtttt gtcggctcca agccttttta atgaaagtga aagcaaggag
     2401 tcagcctatc ttcaagagtt ggtccatttg ggatttagtc gaaaagagaa gaggatgaat
     2461 cacaaaaggc tgtctaagga agatatggga tcctatcaca gtcttttgtc cagtctggtt
     2521 gcctatcacc agcagggcga gacgagtgat actgagcaag atttgacctt tgtcaaggtt
     2581 ctggcgcgtg tgatgggtaa aaaactagac cagcaaggtt tggaaaatcc tgcccttcca
     2641 accagtccaa gcagtaagca gttgaccaaa gataccttac aagctctcta tcctgctgaa
     2701 caggaatttt acctatctac gtccggtttg accgagtttt acagaaacca atacagttat
     2761 ttcctccgct acgtgttagg cttgcaggag gaattacgtt tgcgtccgga tgctcgcagt
     2821 catggaaatt tcttgcaccg catttttgaa cgtgccttgc agttgcctga tgaagattcc
     2881 tttgacaacc gtctagaaca agctattcaa gaaactagtc aagaacgcga atttgaagct
     2941 atttatcaag aaagtttgga agcccagttt accaaagaag ttttgcttga tgttgcacgg
     3001 acgactggtc acattctccg ccataatcca gtcatcgaaa ccattaaaga agaggcaaat
     3061 ttcggtggga aagagcaagc ctttattcaa ttggacaatg gacgcagtgt ctttgtacga
     3121 ggtaaggtgg accggattga ccgtttgaaa gctgatggag cgataggagt agtagactac
     3181 aagtcgagtc taactcagtt ccagtttcct catttcttta atggactcaa ttcccaactg
     3241 ccaacctatc tagctgccct aaaaagagaa ggggagcaga actttttcgg tgctatgtac
     3301 ttggaaatgg ctgagcctgt ccaatctttg atggccgtta aaagtctggc aggtgcagta
     3361 gtagaagcca gcaagtcaat gaaataccaa ggcctctttt tagaaaaaga aagcagtcat
     3421 ttgggcgaat tttacaataa aaacaaggcc aatcagctga cggatgagga attccagctc
     3481 ctactggact acaatgccca tctttacaag aaagcggctg agaagatttt agaaggccag
     3541 ttcgctatca atccctatac cgaaaatggc agaagcattg ctccatgcgt ccagcaacat
     3601 caagccatca caggctttga agccaattac catttgggtc aagcccgttt cctagaaaag
     3661 ttggacttag ctgatggcaa gcgtctggtt ggagaaaaac tcaagcaagc ttggcttgaa
     3721 aaaataagag aggagttgaa tcgatgaagc ttattccctt tttaagtgag gaggagattc
     3781 aaaaactgca agaagcagaa gcaaattcga gcaaggaaca gaagaaaact gccgagcaaa
     3841 tcgaagctat ctacacttct ggccagaata tcctggtctc agcatcggct ggttctggaa
     3901 agacctttgt catggcagag cgcattctgg accaattggc gcgtggtgtc gaaatttctc
     3961 aactctttat ctcaaccttt accgtcaagg ctgcaactga acttaaagaa cgtttagaga
     4021 aaaaaatcag caagaaaatc caagaaacag atgatgtcga gctcaaacaa cacttgggtc
     4081 gccagttggc agacctaccc aacgctgcca tcggaaccat ggactctttc acacaaaaat
     4141 tccttggcaa acatggttat ctgcttgata ttgcacctaa tttccgtatt ttacaaaacc
     4201 aaagcgagca acttcttctc gaaaacgaag tctttcatga ggtctttgaa gcgcattacc
     4261 aaggtaaaca gaaagagacc tttagtcatt tgctgaaaaa ctttgctggg cgtggcaagg
     4321 acgaacgggg tctgcgccag caggtctata aaatctatga cttcctccaa tccaccagca
     4381 atcctcaaaa gtggctgagt gactctttcc tcaaaggctt tgagaaagct gattttacca
     4441 gtgaaaaaga aaaactgact gagcaaatca aacaagccct ttgggatttg gaaagctttt
     4501 tccgttacca tctggataac gatgccaagg agtttgcaaa ggctgcctat ttagaaaatg
     4561 ttcagttaat tctggatgaa attggctccc taaatcagga gtccgatagt caggcttatc
     4621 aggcagtgct tgcgcatgtt gtcgccatct ctaaggagaa aaacggtcga gctctgacta
     4681 atgccagccg taaggctgat ttgaagcccc tggctgatac ctacaacgaa gagagaaaga
     4741 cccagtttgc taaactagga caattatcag accagatagc gattctcgac tatcaagaac
     4801 gttatcatgg agacacttgg aaactagcta aaaccttcca atctttcatg agcgattttg
     4861 tagaggctta tcgtcagaga aaacgacagg aaaatgcctt tgaattcgct gatatcagcc
     4921 attacaccat tgagatttta gagaatttcc cacaagttcg tgagtcttat caggagcgct
     4981 tccatgaagt catggtcgat gagtatcagg ataccaacca tattcaagaa cggatgctgg
     5041 aattgttgtc taatggccac aatcgcttta tggtgggaga tatcaagcaa tccatctatc
     5101 gtttccgtca ggcagacccg cagattttca atgagaaatt ccaacgctat gcgcaaaatc
     5161 cccaagaagg caagctcatt ctcctcaagg aaaatttccg tagtagttca gaagtgctgt
     5221 cagcaaccaa tgatgtcttt gaacgtctca tggaccaaga ggtcggcgaa atcaactatg
     5281 ataacaagca ccagcttgtt tttgccaata ccaaactgac tcccaatcca gacaacaagg
     5341 cagaatttct cctctacgac aaggatgata caggtgagga agaagagagt caaacagaaa
     5401 cgaaactaac aggcgaaatg cgcttagtta tcaaggagat tctgaaactt catcaggaaa
     5461 aaggtgttgc ctttaaggaa attgcccttc taacctccag ccgcagtcgt aatgaccaga
     5521 ttctcctcgc cctgtctgag tacgggattc ctgtcaaaac tgacggagag caaaacaatt
     5581 atctccaatc cctagaagtg caagtcatgc tagacactct tcgtgtcatt cacaatcccc
     5641 tgcaagacta cgccttggtt gcccttatga agtctccaat gtttggtttt gatgaggatg
     5701 agctagcacg tttgtccctt cagaaagcag aggataaagt ccacgaaaat ctctatgaga
     5761 aactggtcaa tgcacaaaaa atggcaagta gtcaaaaagg cttgattcac acagctctag
     5821 ctgaaaaact aaagcaattc atggatatcc tagcttcttg gcgcttgtat gccaaaaccc
     5881 attctctcta tgacttgatt tggaagattt acaacgaccg tttttattat gactatgttg
     5941 gggctttgcc gaatggtcct gctaggcagg ccaatctcta tgccctagca ctgcgtgctg
     6001 atcaatttga aaagagcaat ttcaaaggtt tgtcgcgttt tattcgtatg attgaccaag
     6061 tcttagaagc ccagcacgat ttggcaagcg tggtcgtcgc accgccaaaa gatgcagtag
     6121 agctcatgac catccacaag agtaaagggc tggagtttcc ttacgtcttt atcctcaata
     6181 tggatcaaga tttcaacaag caagactcta tgtcagaagt cattctcagt cgtcagaatg
     6241 gtcttggtgt caaatatatt gccaagatgg agacaggggc agtagaagac cactatccta
     6301 aaaccatcaa actctccatt cctagtctga cctataggca gaacgaagag gaattacagc
     6361 tagcaagcta ttctgagcag atgcgtttgc tgtatgttgc tatgacgcgg gctgagaaaa
     6421 agctctatct tgtcggcaag ggttctcgtg aaaagctgga atccaaggaa tacccagcag
     6481 ccaaaaatgg gaaactaaat agcaatacta gactgcaagc acggaatttc caagattggc
     6541 tttgggctat cagtaaagtg tttactaagg acaagctcaa ctttagttat cgttttattg
     6601 gcgaagatca gttgaccaga gaagctatcg gagagttgga aaccaagagt cctctccaag
     6661 atagctccca agcagacaat cgtcagtcag ataccatcaa agaagctctg gaaatgctga
     6721 aggaggtgga agtttataat actcttcacc gcgcagctat tgaacttcct agtgttcaaa
     6781 ccccaagtca aatcaagaaa ttctacgaac cagttatgga tatggaaggt gtcgagattg
     6841 ctggtcaagg tcagtcagta ggcaagaaaa tcagcttcga tttgccagat ttttcaacca
     6901 aagaaaaggt aactggagct gagattggta gtgctactca cgaactcatg cagagaattg
     6961 acctcagcca gcaactaacc cttgctagcc taacagaaac actcaaacaa gttcaaacta
     7021 gccaagctgt cagagacaag atcaatcttg ataaaattct tgctttcttt gacacagcac
     7081 tcggtcagga aatttttgct aataccgacc atctctatcg cgagcaacct ttctccatgc
     7141 tcaaacgaga ccaaaagagt caggaagact ttgttgtccg tggtatcctt gatggctatc
     7201 tgctttacga aaacaaaatt gttttgttcg actacaagac agaccgctat gatgaaccaa
     7261 gtcaactcgt agaccgctat cgtggtcagt tagctctata cgaagaggct ttatcacgag
     7321 cctattcgat tgaaaatatt gaaaaatact tgattttact cggtaaagac gaggttcaag
     7381 ttgtaaaagt ataacttata ctcttcgaaa atctcttcaa accacgtcag cttcgccttg
     7441 ctgtatatat ggttactgac ttcgtcagtt ctatccacaa cctcaaaaca ctgttttgag
     7501 ctgactgcgg ctagctttct agtttgctct ttgattttta ttgagtatta gaaaggagac
     7561 ctcatgacac ttccagttag aaaatccctg cacgatgcag ttttacaggc ttcagaagcg
     7621 gatacttggg aacaagctac caaggaatgg aatgaagttt ccttgatttt taacggtatc
     7681 ggccgtagca attgtgtctg tggaaatgtc atcaaatacg cttatgaact ctttaacggt
     7741 gtcacaggcc aacgcctctt tccaattggt agcgactgtg ttcgtcattt tcatcgattg
     7801 tcccttgacc agcaattgga agaggaagaa aaactgctca gaaaggttga aaatctaacc
     7861 agaaaagctc agaaaaagga aaaaatcaag gtcaataaaa gtgactttga cgagcgactt
     7921 ctaaaatggc tctgggaaaa aggtgttttc aaacccaatc gtggcaatca atttgcgcct
     7981 gagagagact accagctttt cctagaagtc tttcagggag gaagttggac caaggcggaa
     8041 ccaaagaaaa aggctcggat ggaagaagtc cttgaaaagt gtatcaaacc ctttttactt
     8101 gggaaatcgg atgaccaact ctaccttgtc aagctaggca aggagaaaat tgactacgaa
     8161 caagaactcc gtatccaggc agagaaagaa cgcaagaaga gggataaaat cgccaagcaa
     8221 tatgcagata atctggttct agccatggga cccgcagagc gtgcttatca agattacttt
     8281 ggctttacag agaccctgac tcaagaagaa cgcaagtggg aaaaaattct ttttggtaaa
     8341 aatagagcag aacgggctat caaggccaaa caataccaaa aagagctgga aaaagaccaa
     8401 cgaattgcca gtcaggatcc aattgaaaga aaacagaagc agacttggct cctcaattcc
     8461 tattttcgtg agcttcctga agaaaaagct cgattttcta ggctcttatt agaatatcga
     8521 aaaagtggtg aagtaccctt ttcaactgaa tacctgtccg atcatctcat cgactttttc
     8581 tataaaatga aagcctttga atttgaaatc gcaccagaac aagtccgaga ttttctaaaa
     8641 aaaagcctgc aggaagacca tcgctcatca gcacaaggaa gctggataga aggaattctc
     8701 ctcaactgcc tcaaaccatt tttagaacga ttagtgatat gagtaacacc taccgtgtaa
     8761 atttcatggt aggtgttatt gtaccccaaa ataatataaa acaaagtatg aaagcgcatc
     8821 tcaattttaa gacttttttg taaattaaga gtataattaa ggtatactgc cttttctata
     8881 gataatagat aatacactaa tataataaag gtctttgtaa catatataaa ttttatttca
     8941 aggaggaata atggaaaagt attttggtga aaaacaagag cgtttttcat ttagaaaatt
     9001 atcagtagga cttgtatctg caacgatttc aagtttattt tttatgtctg tattagctag
     9061 ttcatctgtg gatgctcaag aaactgcggg agttcactat aaatatgtgg cagattcaga
     9121 gctatcatca gaagaaaaga agcagcttgt ctatgatatt ccgacatacg tggagaatga
     9181 tgatgaaact tattatcttg tttataagtt aaattctcaa aatcaactgg cggaattacc
     9241 aaatactgga agcaagaatg agaggcaagc cctagttgct ggtgctagct tagctgctct
     9301 gggaatttta atttttgctg tttccaagaa aaaggttaag aataaaacgg tattacattt
     9361 agtattggtt gcgggaatag gaaatggtgt cttagtttca gtccatgctt tagaaaatca
     9421 tcttttgcta aattacaata cggactatga attgacctct ggagaaaaat tacctcttcc
     9481 taaagagatt tcaggttaca cttatattgg atatatcaaa gagggaaaaa cgacttctga
     9541 ttttgaagta agtaatcaag aaaaatcagc agccactcct acaaaacaac aaaaggtgga
     9601 ttataatgtt acaccaaatt ttgtagacca tccatcaaca gtacaagcta ttcaggaaca
     9661 aacacctgtt tcttcaacta agccgacaga agttcaagta gttgaaaaac ctttctctac
     9721 tgaattaatc aatccaagaa aagaagagaa acaatcttca gattctcaag aacaattagc
     9781 cgaacataag aatctagaaa cgaagaaaga ggagaagatt tctccaaaag aaaagactgg
     9841 ggtaaataca ttaaatccac aggatgaagt tttatcaggt caattgaaca aacctgaact
     9901 cttatatcgt gaggaaacta tagagacaaa aatagatttt caagaagaaa ttcaagaaaa
     9961 tcctgattta gctgaaggaa ctgtaagagt aaaacaagaa ggtaaattag gtaagaaagt
    10021 tgaaatcgtc agaatattct ctgtaaacaa ggaagaagtt tcgcgagaaa ttgtttcaac
    10081 ttcaacgact gcgcctagtc caagaatagt cgaaaaaggt actaaaaaaa ctcaagttat
    10141 aaaggaacaa cctgagactg gtgtagaaca taaggacgta cagtctggag ctattgttga
    10201 acccgcaatt cagcctgagt tgcccgaagc tgtagtaagt gacaaaggcg aaccagaagt
    10261 tcaacctaca ttacccgaag cagttgtgac cgacaaaggt gagactga
//
LOCUS       DAVKQL010000071        10271 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVKQL010000071 DAVKQL010000000
VERSION     DAVKQL010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10271
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.071"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            89..1264
                     /gene="mutY"
                     /locus_tag="VW561_001830"
     CDS             89..1264
                     /gene="mutY"
                     /locus_tag="VW561_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEU9944052.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            1319..2023
                     /locus_tag="VW561_001831"
     CDS             1319..2023
                     /locus_tag="VW561_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9944053.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            2016..3365
                     /gene="vicK"
                     /locus_tag="VW561_001832"
     CDS             2016..3365
                     /gene="vicK"
                     /locus_tag="VW561_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEU9944054.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            3367..4176
                     /locus_tag="VW561_001833"
     CDS             3367..4176
                     /locus_tag="VW561_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU9944055.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            4267..4509
                     /locus_tag="VW561_001834"
     CDS             4267..4509
                     /locus_tag="VW561_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEU9944056.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            4511..4765
                     /locus_tag="VW561_001835"
     CDS             4511..4765
                     /locus_tag="VW561_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU9944057.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            4812..5585
                     /locus_tag="VW561_001836"
     CDS             4812..5585
                     /locus_tag="VW561_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEU9944058.1"
                     /translation="MKKKWFFADYYDTTIILLALISVILVLLGFAEMIDLDNPPYSII
                     DLVIWGVFVIDYSWRFFTTKRKWRFILENIFDLLAILPLNAIFTVFRLGRIFRLAKLT
                     KLLKLTRLLRIIGLTGKLERKISRFLRTNGLIYILYVNIFIVLVGSSILSVVEEKSFS
                     DSLWWALVTVTTVGYGDIVPASIFGKWLAVLLMLVGIGTIGMLTSALTNFFVKDNPDE
                     QIKLDKLQDELSSQRILLEKQSKKIEELHKMIQDLIEKT"
     gene            complement(5848..6834)
                     /locus_tag="VW561_001837"
     CDS             complement(5848..6834)
                     /locus_tag="VW561_001837"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEU9944059.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            7028..9496
                     /gene="gyrA"
                     /locus_tag="VW561_001838"
     CDS             7028..9496
                     /gene="gyrA"
                     /locus_tag="VW561_001838"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU9944060.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMVGQILSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            9496..10239
                     /gene="srtA"
                     /locus_tag="VW561_001839"
     CDS             9496..10239
                     /gene="srtA"
                     /locus_tag="VW561_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEU9944061.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTAFNQPYKQFY"
BASE COUNT         3178 a         1737 c         2301 g         3055 t
ORIGIN      
        1 agtagataga aaagttatat actatctatt gaaaatatta taaatcagat acaaagacag
       61 gtgaaacctt ctgtgttata ctagagatat gttagatttg aaagaatacg gtatcgtcat
      121 gtggccggag gagaaagtca tttctttccg tgagaaactt ctcgcttggt atgatgaaaa
      181 caaaagagat ttgccttgga ggagaagtaa aaatccttat cacatctggg tatctgaaat
      241 catgcttcag cagaccaggg tggatacagt tatcccttac tacgaaagat ttttggactg
      301 gtttccaact gtcgaaagtc tggcaactgc gcctgaggag agtttactga aagcttggga
      361 gggcttgggc tattattctc gagttcgcaa tatgcaggct gcagcccagc agattatgac
      421 tgactttggt ggccaatttc caaataccta tgaaggaatt tccagcttga aagggattgg
      481 accttacaca gcaggagcca tttccagtat tgcttttaac ttgcctgagc cagctgtaga
      541 tggtaatgtc atgcgggtct tggcgcgtct gtttgaagtc aaccacgata ttgggattcc
      601 aagtaatcga aaaatttttc aggcaatgat ggagatcttg attaatccgg atcgtccggg
      661 tgattttaat caagcattga tggacttagg gtctgatatt gagtctcctg taaatcccag
      721 acctgaagaa agcccagtca aggactttag tgcggcatat cagaatggaa ccatggaccg
      781 ttatccaatt aaatctccta agaaaaagcc ggttcctatt tatcttaaag ccttagttgt
      841 gaaaaattct caaggacaat ttttacttga aaaaaatgaa agcgaaaagc tcttagcagg
      901 tttttggcat ttccccttta tagaagttga taacttttcg caagaagagc agtttgacct
      961 ctttcatcag gttgcggaag aaagtgtgaa ctttggcccc agtccagaag agagtttcca
     1021 gcaggactat gacctagatg ttgattggct tgatgtttgt tttgatacag tccagcatgt
     1081 ttttagtcat cgaaaatggc atgtacagat tgtagcaggt caggtgagtg acttccatga
     1141 tttttcagat agggaagttc gttggctttc accagaagaa ttcaagaatt acccacttgc
     1201 caaaccccaa caaaaaatct ggcaggctta tgcacaagcc aacttagaca gtagccaaga
     1261 ctagctattg tgtcttctga ctattttttg gtataatagt agagaaaaag gtgaacatat
     1321 gaaaaaaata ctaattgtag atgatgagaa accaatctcg gatattatca agtttaatat
     1381 gaccaaggaa ggttacgaag ttgtaactgc ttttaatggt cgtgaagcgc tagagcaatt
     1441 tgaagcagag caaccagata ttattattct ggatttgatg cttccagaaa ttgatggttt
     1501 agaagttgct aagaccattc gtaagacaag cagtgtgccc attcttatgc tttcagccaa
     1561 agatagtgaa tttgataagg ttatcggttt ggaacttggg gcagatgact atgtaacgaa
     1621 acccttttcc aatcgtgagt tgcaggcgcg tgttaaagct cttctgcgtc gttctcaacc
     1681 tatgccagta gatggtcagg aagcagatag taaacctcaa cctatccaaa ttggggattt
     1741 agaaattgtt ccagacgcct acgtggctaa aaaatatggc gaagaactag acttaaccca
     1801 tcgtgaattt gagcttttgt atcatttagc atcgcataca ggtcaagtca tcacgcgcga
     1861 acacttgctt gagactgtct ggggttatga ctattttggt gatgtccgca cagttgatgt
     1921 gactgtacga cgtctgcgtg agaagattga agatacgccc agccgaccag agtatatctt
     1981 gacgcgccgt ggtgtagggt attacatgag aaataatgct tgatttactg aaacaaacca
     2041 tttttaccag agattttatc tttatcctga ttttgttagg tttcatcctt gttgtgaccc
     2101 tcttattact ggaaaataga cgtgataata ttcagttgaa gcaaatcaat caaaaggtta
     2161 aagatttgat tgcaggagat tattccaagg ttcttgatat gcaaggtggg tctgaaatca
     2221 ccaatattac caataatttg aatgacttgt cggaggttat tcgtctcact caggaaaatc
     2281 tagaacaaga gagtaagagg ctaaatagta ttctgtttta tatgacagat ggggttcttg
     2341 cgactaaccg tcggggtcag attatcatga ttaacgatac agccaagaag caactggggt
     2401 tggttaagga agatgttctg aatagaagca ttttggaatt gctcaagata gaagaaaact
     2461 atgaattgcg tgatttgatt acccaaagtc cagaattgtt gctagattcc caagatatca
     2521 atggtgaata tttgaacctt cgagttcgct ttgccttgat acgtcgagag tctggcttta
     2581 tttcaggttt ggtggctgtt ttgcatgata cgacggagca ggagaaggaa gaacgcgaac
     2641 gaagactctt tgtttccaat gttagccatg agttacggac tcctctgact agcgtaaaat
     2701 cctatcttga agccttggat gagggggctt tgtgtgaaac tgtagcacca gactttatca
     2761 aggtttctct tgatgagacc aaccgtatga tgcgcatggt gacggatctc ctccatcttt
     2821 cacgtattga taatgctacc agtcacctag atgtggaact gattaacttc actgctttta
     2881 ttacctttat cctcaatcgt tttgacaaga tgaaaggaca ggaaaaggag aaaaaatatg
     2941 agttggtgag agattatccc atcaattcta tctggatgga aattgataca gataagatga
     3001 cgcaggttgt cgacaatatt ttaaataatg ctattaagta ttcgccagat gggggtaaaa
     3061 tcactgtcag aatgaagaca actgaagacc agatgatttt atccatttct gaccacggtt
     3121 tggggattcc taagcaggat ttaccacgta tctttgaccg tttctatcgt gtggatcgtg
     3181 ctagaagtcg tgcacaaggt ggtacaggtc taggactgtc tatcgctaaa gaaattatta
     3241 aacaacataa gggctttatt tgggccaaga gtgaatacgg caagggttca acctttacca
     3301 ttgtgctccc ttatgataag gatgcagtga aagaagaagt atgggaggat gaagtagaag
     3361 actagaatga gtgaaatagg ctttaaatac agtattttag cgtcgggttc cagtggaaat
     3421 tctttttatc tggaaacctc aaaaaagaag cttttagtag atgcaggctt gtctggcaag
     3481 aaaattacca gtctgctagc tgaaattaac cgtaagccag aagacctgga tgccatcttg
     3541 attacccatg agcattcaga tcatatccat ggagtaggcg ttttggctcg caagtatggt
     3601 atggatcttt atgccaatga aaagacctgg caagctatgg aaaatagtaa atatcttggc
     3661 aaggtggatt cttcgcaaaa gcatattttt gaaatgggta aaaccaaaac ctttggagat
     3721 atcgacatcg agagttttgg tgtaagccat gatgcagtcg caccgcagtt ctatcgcttt
     3781 atgaaggatg ataagagttt tgtcctcttg acagatacag gttatgtcag tgaccgtatg
     3841 gcgggcattg tcgaaaatgc ggatggttat cttatcgagg ccaaccatga tgtagagatt
     3901 ttgcgatcag gttcttacgc ttggcgcctc aaacaacgaa tcctatctga ccttggtcac
     3961 ctttctaacg aggacggtgc tgaagctatg attcggacgc taggaaatcg tactaagaag
     4021 atttaccttg ggcatttatc taaggaaaac aatatcaagg aactggctca tatgaccatg
     4081 gtcaatcagc tggctcaagc tgatctggga gtcggagtag actttaaggt ttatgatacc
     4141 tcaccagata ccgcaacacc attgacagag atatagaaag aacgctgaga aggtgttctt
     4201 tttatattga ctggacacct aaaaagtaat acaatggtgt taccattaaa aaagggagca
     4261 caaaagatga ctactattac attaaaagtt tctgaagctg ataaaacatt tatgaaagca
     4321 atggctaagt ttgaaggagt ttccctttcg gaacttattc gcaccaaaac tcttgaagcc
     4381 ctagaagatg aatacgatgc tcgtgtggca gatttagcct atcaagagta tttagaagac
     4441 ttggaaaaag gagttgaacc cattacttgg gaagaaatga tgcatgattt aggcttgaag
     4501 gatgaataat ttgtataaat tagttccaac aagacgtttt atcaagcaat tgaaaaaatt
     4561 ggaccgttat acgcagaagc taattacaaa ctatttacaa accaatgttt tggaagatcc
     4621 aagacgacac ggaaaggctt tggttggtaa tcgcgttggt caatggcgct atagaattgg
     4681 taattatcga gttatcgtac aaattgtaga tgatgaatta gttgttgcta ctctagaagt
     4741 tggtcatcgg agagatattt attaaattaa agctgagtaa taacagtata gaaagtaagg
     4801 agagtaaaaa tttgaagaaa aaatggtttt ttgcagatta ttatgacaca acaattattt
     4861 tacttgcttt aatctctgtt attctggtat tacttggatt tgctgagatg attgatttgg
     4921 acaatccgcc ttatagtatt attgatttag taatctgggg tgtttttgta attgattata
     4981 gttggcgctt ttttacgact aaaagaaaat ggcgctttat tcttgaaaat atttttgatt
     5041 tattggctat tctcccttta aatgctattt ttacagtgtt tcgattaggg cgtatctttc
     5101 gtttagctaa actaacaaaa ttactgaaac tgactcgttt acttagaata attggactta
     5161 caggtaaatt agaaagaaaa attagtagat tcttacggac aaatggcctg atttatatct
     5221 tgtatgttaa tatctttatt gttctagtag ggagttcgat tttatccgtt gttgaagaaa
     5281 aatccttctc agatagtctt tggtgggctc ttgtgacagt aactactgtt ggatatgggg
     5341 atattgttcc tgcttccatt tttgggaaat ggctagcagt tttactgatg cttgtcggta
     5401 ttggaacaat agggatgtta acaagtgctt tgacgaactt ttttgtaaag gataatccag
     5461 atgaacagat aaaacttgat aaactccaag atgaattatc tagtcagaga atattactag
     5521 agaaacaatc taaaaagata gaagaactac ataaaatgat acaagatttg attgaaaaaa
     5581 cataataaaa ataaagatga gaaaaagttt gaactgagga gaaatcaagt aagttttgac
     5641 acaatagaac gcatattatc aggtataaaa acttgatact atgcgtttta ttgtgggaag
     5701 atgtataatg gattgaaata agatatgaac aaatcgatta ggaatttaaa gcattttata
     5761 acaacgtttt agagtaacgg ggtactattt catataatac aggaaaaaac aactccctga
     5821 tgattcaagg agttgtctat agttaaatta gtttttagaa gcttcttgga attctggatt
     5881 tttccatgct tcgtcaataa tagcttgcaa ttctttagca gacgcttgca ttttttgagt
     5941 ttctgcatcg ttcaatggaa tgtttactgg acgaacgata ccatgtgcac caacaacagc
     6001 tggttgaccg ataaagacat tctcaactcc gtattgacct tcttggaata ctgaaagtgg
     6061 aagtactgcg ttttcatcgt caaggattgc tttagtgata cgagcaaggg ctactgcgat
     6121 accgtagtat gttgcacctt ttttattgat gattgtgtag gctgcatcac gaacaccttc
     6181 gaacaattca atcaattcag cttcttgaac attttgagtg tctttaagga attcttcaag
     6241 gtttacacca gcgatgttag cgtgtgacca aacagcgaac tcagagtcac cgtgttcacc
     6301 catgatgtag gcgtgcactg aacgagcatc cacatccaat ttttcagcaa gtgcttgacg
     6361 gaaacgagct gagtcaagtg aagtacctga accgataacg cgttctttag ggaaaccaga
     6421 gaatttccaa gttgagtaag tcaaaacgtc aactggatta gcagcaacaa ggaagatacc
     6481 tttgaaacca gattcaacaa cttgagttac gattgatttg ttgatagcaa ggtttttacc
     6541 tacaaggtca agacgagttt cacctggttt ttgaggtgca cctgcagtga tcacaacaag
     6601 gtcagcgtct gcacagtcag agtattgagc tgcatagatt tttttaggtg aagtgaaggc
     6661 aagggcgtga ctaaggtcaa gcgcatcacc aacagctttt tcatgcaatt gtggaatttc
     6721 gataattcca agctcttgtg caattccttg gttaacaagt gcaaaagcgt aagatgaacc
     6781 tacagcacca tcaccgacaa ggataacttt tttgtgttgt ttagttgaag tcattgtttt
     6841 aaacatctcc ttaattttat taggggattt tccctggaca acttcattct atcactttta
     6901 aaaaactttg tcacgaatat gccttatagt tctcgatgta aacgttttag tggtttagag
     6961 gctgaaatag atgggaattt atggtataat gttgttactt actaattgtg aaatgaggca
     7021 tttattaatg caggataaaa atttagtgaa tgtcaatctg acaaaggaga tgaaggcaag
     7081 ttttatcgac tacgccatga gtgttatcgt agcgcgagct cttcctgatg ttcgagatgg
     7141 cttaaaacct gttcaccgtc gcattctcta cggaatgaat gaattgggtg tgaccccaga
     7201 caaaccccat aaaaaatctg ctcgtattac aggggatgtc atgggtaaat atcacccaca
     7261 cggggattcc tctatttatg aagccatggt ccgtatggct caatggtgga gctaccgtta
     7321 catgcttgta gatggtcatg ggaattttgg ttccatggat ggagatagtg ctgccgctca
     7381 acgttatacc gaggcacgta tgagcaagat tgctctggaa atgcttcgtg atatcaacaa
     7441 aaatacagtt gatttcgttg ataactatga tgccaatgaa cgggaaccct tggtcttgcc
     7501 agcgcgtttt ccaaaccttt tggttaatgg agcaactggt atcgcggttg ggatggcaac
     7561 caatattcca cctcataatc tgggtgaaac cattgatgca gtgaagttgg tcatggataa
     7621 tcctgaagtg actaccaagg acttgatgga agtcttgcct ggaccagatt ttccaactgg
     7681 tgctcttgtc atggggaaat caggtatcca taaggcttat gaaacaggta aaggttcgat
     7741 tgtcctacgt tctcgtacag agattgaaac gactaagact ggtcgtgagc gtatcgttgt
     7801 aacagaattt ccttacatgg tcaataaaac caaggtgcat gagcatattg ttcgcttggt
     7861 tcaggaaaaa cgcattgagg gtatcacagc agtacgtgat gagtcaaacc gtgaaggtgt
     7921 tcgatttgtt attgaagtca agcgcgacgc ctcagccaat gttattctca ataacctctt
     7981 caaaatgacc caaatgcaaa ccaattttgg tttcaatatg ctcgctatcc aaaatggtat
     8041 accgaaaatt ttgtctcttc gtcagatttt ggatgcttat atcgagcacc aaaaagaagt
     8101 ggttgttcgt cgtacacgtt ttgataagga aaaagcggaa gcgcgcgctc atatcttaga
     8161 aggtctcttg attgcgctag accatatcga cgaagtgatt cgtatcatcc gtgctagtga
     8221 aacggatgcg gaagctcaag ctgagttgat gagcaagttt aagctttctg aacgtcaaag
     8281 tcaagctatc cttgatatgc gtcttcgtcg tttgacaggt ttggaacgcg ataagattca
     8341 atctgagtat gatgacctct tggctctgat tgcggattta gcagatattc ttgctaagcc
     8401 tgaacgtgtt tctcaaatta tcaaagacga attggatgaa gttaaacgta aattttctga
     8461 taaacgccgt acagagttga tggttggaca gatcttgagt ctcgaggatg aggacttgat
     8521 tgaagaatcg gatgtcttga ttaccctttc taacagaggc tacattaagc gtttggatca
     8581 ggacgagttc actgctcaaa aacgtggggg tcgtggtgtc caaggaacgg gagtgaaaga
     8641 tgatgacttt gttcgtgagt tagtgtcaac tagcacacat gatcatctgc tcttcttcac
     8701 aaacaaggga cgtgtctatc gtcttaaagg ttatgaaatt cctgagtatg gtcggactgc
     8761 caaagggcta ccagtagtca atctcttgaa attggatgaa gacgaaagta ttcagacggt
     8821 tatcaatgtt gagtctgatc gcagtgatga tgcttatctc ttctttacaa cccgtcacgg
     8881 tattgtgaag agaactagtg ttaaggagtt tgccaatatt cgtcaaaatg gtctcaaagc
     8941 gctgaattta aaggatgaag atgagttaat caatgtcttg ttggcagaag gagatatgga
     9001 tattatcatt ggtaccaagt ttggttatgc agttcgcttt aatcaatcag ccgttcgtgg
     9061 tatgagccgt atcgccactg gtgtgaaagg tgttaacctt cgtgaaggag acacagttgt
     9121 tggtgccagc ttgattactg atcaagatga ggttcttatt atcacagaaa aaggatatgg
     9181 taagcgtaca gtcgctactg aatacccaac aaaaggtcgt ggtggtaagg gaatgcagac
     9241 agctaaaatt accgaaaaaa atggcttgct ggccggtctt atgactgttc aaggggatga
     9301 ggatttgatg attatcactg atacaggtgt catgattcga accaatcttg ccaatatttc
     9361 acaaacagga cgtgcaacta tgggagttaa agtaatgcgc ctggatcaag atgctcagat
     9421 agtgactttc acaacggttg cggtggcaga aaaagaagaa gttgggacag aaaacgaaac
     9481 agaaggtgaa gcataatgtc tcaaaaaaat aataaaaaga aaaacaagcg aaaaaatctg
     9541 ctgacaaata tcctagcagg atttctgata ttactgtcac tggctttgat ttttaatact
     9601 caaattcgaa atattttcat agtctggaat accaataagt atcaagttag ccaggtatca
     9661 aaagaaaaat tagaagaaaa tcaggataca gaaggcaatt ttgactttga ttctgtcaaa
     9721 gctatctctt cggaagctgt tctaacttct caatgggatg ctcaaaaatt accagttatt
     9781 gggggaattg caattcctga attggaaatg aatttgccga tttttaaagg acttgataat
     9841 gttaatctct tctacggagc tggtacaatg aaacgcgagc aagtaatggg agaaggaaat
     9901 tatagtctag ctagtcacca tatctttggt gttgataatg ctaataaaat gttattttct
     9961 cctttagata atgctaaaaa tggcatgaag atttatctaa ccgataaaaa taaagtttat
    10021 acttatgaaa tacgtgaagt caaacgtgtg acaccggatc gtgttgatga agttgatgat
    10081 agagatgggg tcaatgaaat cacattagta acctgtgaag accttgctgc tacagaacgt
    10141 attattgtca aaggtgattt gaaagaaaca aaagattatt cacaaacatc tgatgaaatc
    10201 ctaacagctt tcaatcaacc atataaacaa ttttattaat acaaatcagt gaaatcatgg
    10261 atttcactga t
//
LOCUS       DAVKQL010000072        10214 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVKQL010000072 DAVKQL010000000
VERSION     DAVKQL010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10214
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.072"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            122..1792
                     /locus_tag="VW561_001840"
     CDS             122..1792
                     /locus_tag="VW561_001840"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEU9944062.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNALSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            complement(2283..3449)
                     /locus_tag="VW561_001841"
     CDS             complement(2283..3449)
                     /locus_tag="VW561_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU9944063.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFHVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASEFEPQYCLATSRRANAHYVY
                     SEENLLAKYSQQFKVKKIMPEAFAELQGDYFYMLTTPSRQEMDQMIADFGQRQE"
     gene            complement(3462..4201)
                     /locus_tag="VW561_001842"
                     /pseudo
     CDS             complement(3462..4201)
                     /locus_tag="VW561_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            complement(4223..4810)
                     /locus_tag="VW561_001843"
     CDS             complement(4223..4810)
                     /locus_tag="VW561_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9944064.1"
                     /translation="MIVVAIDNDGMGRMNEYAAWKFQEFPIPEQQFGGKGVEYAEFVM
                     EVVKPFIDETYRTKADCQHTAMIGSSLGGNITQFIGLEYQDQIGCLGVFSSANWLHQE
                     AFNRYIECQKLSPDQRIFIYVGTEEADDTDKTLMDGNIKQAYIDSSLCYYHDLIAGGV
                     HLDNLVLKVQSGAIHSEIPWSENLPDCLRFFAEKW"
     gene            complement(4791..5055)
                     /locus_tag="VW561_001844"
                     /pseudo
     CDS             complement(4791..5055)
                     /locus_tag="VW561_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853191.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(5191..6405)
                     /gene="thiI"
                     /locus_tag="VW561_001845"
     CDS             complement(5191..6405)
                     /gene="thiI"
                     /locus_tag="VW561_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEU9944065.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYPQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSAVQEIMQDIYKE
                     GMTFKISSKRSDHTFELDSRELNRTLGGAVFEAIPNVQAQMKSPDINLQVEIREEAAY
                     LSYETVRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            complement(6414..7556)
                     /locus_tag="VW561_001846"
     CDS             complement(6414..7556)
                     /locus_tag="VW561_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9944066.1"
                     /translation="MIYFDNSATTRPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWIIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKAQGFEVDFAPVDNKGFVDVEALEDLIRPDTTLVSIMAVNNEIGSVQPIEAI
                     SKLLADKPTISFHVDAVQALAKIPTEKYLTERVDFATFSGHKFHGIRGVGFIYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     DYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAQSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            complement(7716..8093)
                     /locus_tag="VW561_001847"
     CDS             complement(7716..8093)
                     /locus_tag="VW561_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944067.1"
                     /translation="MKHKIMKKIIVVVFAIPWLTFLSQVHADYVSKSSSLDGYSLVGL
                     NVLNVTFNASGYSDTYSGSVSNMYFTDWAWFPNTISNRSTWKSSIPYGQRANGTVHIG
                     VGVNSPWGAVNLWGGQNYFSLDF"
     gene            complement(8103..10187)
                     /locus_tag="VW561_001848"
     CDS             complement(8103..10187)
                     /locus_tag="VW561_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9944068.1"
                     /translation="MFEIRNLSLSFAGRRLLEPTDLIFERGKVYTIYGKSGVGKSSLL
                     NKIGLLSATDPSVSYFFDGKEIDIENKKSVSVFIAEEVAFVFQGRNLINDLTVFENLR
                     LALNFYDLSPDEIESKIDTVLADLQISSLKHAYPEDLSGGEEQRVAIARALVTGKTLI
                     LADEPTSSLDKENREKVSALLIDLAHKYHKIVLIVSHDEAMIAIGDVQLHFKDHKLVG
                     NCLILNSDEGIKDSKKWSSCLSSSHTKLSVLKHRRPFLPRLLAFFISLVVAIAVTSIN
                     FQSLFADKYHQLVNNSLENGFLVINDSLHLQTTKVLDDFLSFDKEEITSISTSQNIQT
                     VNPYIEFLSLGMTFDNSKDYSQLQKNFQPTLTIDGQTRSLTRNFSIQPLYQTNTTQRQ
                     IEYFDRSNEKGIYLSEQFISDEKLPNIVSGTTVTLTFYIPISLYESSIEKEGNILKGD
                     GDLFVKVDKTYPVLGIVKKSYPFEYSPYGNTLFMNIAEMEELQNEAIQQHPMTKMALD
                     GFPLKSWMPSAIHVLLKDSGAIPEELSRIRAISSQITILSSYENYEEFNKGLTYIRQF
                     LMLLSLVLLILVIAVLSFVFFLLNRPRRFEVGILKSLGYSTQNIVWLFLKELIGYGKT
                     ISILASCLLVILSILAMQVLKLEISDVFKFYLTSVFTLIGLSVSVLIISGLLPIYTTC
                     RQTVVDTIRKNG"
BASE COUNT         3021 a         2134 c         1972 g         3087 t
ORIGIN      
        1 gaaaggactc taaaagtctt aaaaacagag atatttacga acgtttagag agttttaaga
       61 tgttaaaaat cttgttttgt gatataatga attatcatat aagaggttag aggaattttg
      121 aatgaaaaca gatattgaaa tcgcacagag tattgagttg aagccaattg ttgatgttgt
      181 agagaaactt ggtatttctt acgacgattt ggagttgtac ggaaagtaca aggctaaact
      241 cagctttgat aaaattcggg cagttgagag caatccagtc ggtaaattga tcttggttac
      301 tgccatcaac ccaacacctg caggtgaagg aaagtcgacg cttaccattg gtcttgcgga
      361 tgccttgaac aagattggca agaaaaccat gattgctatc cgcgaaccgt ctcttggtcc
      421 agtcatgggg atcaagggtg gtgctgctgg tggtgggtat gcacaagttc tgccaatgga
      481 agacatcaac ctccacttta ctggagatat gcatgctatt acaactgcca acaatgccct
      541 ttctgccttg attgacaacc acttgcacca agggaatgag ctgggaattg atcaacgtcg
      601 tatcctctgg aaacgcgttg tggacttgaa cgaccgtgcg ctccgccatg tgactgttgg
      661 tcttggtggt cctctaaacg gtattccacg tgaggacggt tttgatatta cagttgcttc
      721 agaaatcatg gcaattcttt gcttggcaac ggacatcgag gacttgaaac gtcgtttggc
      781 gaatatcgtt attggttatc gctatgaccg tacgcctgtt tctgtaggtg atttgcaggt
      841 tgagggtgcc ttggctttga ttttgaagga tgctattaag ccaaacttgg ttcagacaat
      901 ttacggtaca cctgcctttg tacacggtgg tccatttgcc aatatcgctc atggctgtaa
      961 ctctgttttg gcgacgacaa cagcccttca cttggctgat tacactgtta ctgaagctgg
     1021 ttttggtgcg gatcttggtg ctgagaaatt ccttgatatc aagacaccaa acttgccaac
     1081 atctccagat gcagttgtta ttgtcgcaac ccttcgtgcc cttaagatga atggtggtgt
     1141 ggctaaagac gctctgactg aagaaaatgt agaggcagtt cgtgcaggtt ttgctaactt
     1201 gaaacgccac gttgaaaata tccgtaagtt cggtattcca gcagttgtag ctattaacga
     1261 atttgtatct gatacagaag ctgaaattgc agtcttaaaa gaactctgtg cctcaatcga
     1321 tgtaccagtt gaattggcta gtgtctgggc tgatggagca gaaggtggag tagcacttgc
     1381 cgaaacagtt gttaagacaa ttgctgaaaa tccagctaac tataaacgtt tgtacgataa
     1441 tgccctttct gtccaagaaa agattgaaaa aattgtcact gaaatctatc gtggtagcaa
     1501 agtgaacttt gagaagaaag ctcaaacaca aattgctcaa atcgttcaaa acggttggga
     1561 caaattgcca atctgtatgg ctaaaactca atacagtttc tcagacaatc caaatgcgct
     1621 tggagcacca gaaaactttg aaattaccat tcgtgaattg gtaccaaaat taggtgcagg
     1681 cttcatcgtt gccttaactg gtgatgttat gaccatgcca ggtcttccaa aacgtccagc
     1741 agctctcaac atggatgttg aaagcgatgg aactgtacta ggcttgttct agtggattga
     1801 aattgacttt aagtgagttt ggaaataata tccaagctca ttttttctca gactgcaagc
     1861 agttcatcaa taagatttat actcttcgaa aatctcttca aactacgtca gcttcacctt
     1921 gccgtaggta tatgatactg actctgtcag ttctatccac aacctcaaaa cagtgttttg
     1981 agcaacctgc ggctagcttc ctagtttgct ctttgatttt cattgagtat taatagactt
     2041 gtttggtatc ctgaaaacta tatcgtatag gtgtcgtcat ggatcctttt agctgattta
     2101 aggatacgaa aacacaatac tatgactaaa ttgaaatgtt aaaattcaat ttctagccac
     2161 ctaatcctgc ttcaaatagt tatcgaacaa gttttatgat agctaccttg ttaggatgct
     2221 cttatttttt accagacaaa acaaaaggat taagggagtg aatttcctta atccgatagt
     2281 tcttattctt gacgttgtcc gaaatcagca atcatctgat ccatttcttg tcgactcgga
     2341 gttgtcagca tataaaagta atctccttga agttccgcaa aagcctctgg catgattttt
     2401 ttaaccttga actgctggct atatttggca agtaagttct cctcagaata gacatagtga
     2461 gcatttgcac gacgtgatgt agccaaacaa tactgaggtt caaactctga cgctgggaac
     2521 tcttctcctg cgacaatggc tgcatatcca cgatagaggt ccaaggaatg agcaaagtta
     2581 taaacatcaa tggtgaaacc acctgcaggg cggttattgt actcgatggt aatataatcg
     2641 tccccctcac ggaagaactc aatatggaaa aaccgttctt tcataccaaa ttctttgaca
     2701 attgcttccc catacttgcg cagtttagga tccatatcct tgagcacata ataagaattg
     2761 tccatcttat aaatcatgag gtcaagcggt gtataggcgt agtcaaaggt tgttgagaag
     2821 acaatctttc catccttgtc cacgagcccg tcaaaggtac agatttcgct ggaagtgaca
     2881 aatttttcaa agaaataaag ggttgaatgg tcccattctt gcttgaagtg attgatatcg
     2941 tcttctgtct caagtttaaa ggttgcggct gctcccactc cattatcagg tttggcaatc
     3001 attggaagac cgatttcttt cactgcttga tcaacatctg cttccgtctt gataacagct
     3061 ccaggtacca cagggacacc tgcttttttg aaaagtttct tcatttcaga cttatatttc
     3121 gtctttttga gatcctctgg tttggcacca aaaacattga attgttctct gagtgttgcg
     3181 tctagctcaa gccagtattc attgtgagac tcgatgcggt caattggacc atgtttataa
     3241 aagagaaaag caactgcacg tttgacttca tctatgttct caagattatc aacatgaaaa
     3301 tactcggtca agctattgcg caagggctca tccaattgct cgtaagactc ttgaccaatt
     3361 cccaagactg tgatgccttt attagctagt tcgatggtaa actgttgaaa gttttgtgga
     3421 tagtagggag aaataacaag gtaattcata ggtaactcct tttataaata gagattaccg
     3481 aggaaataag gcatttgttt acgccaccat tcccagtcat gggcgacatc atgtccccat
     3541 tcagcaaacc aggctggaat ttgtttcttg tcaaaggctt ctttgagctt gtaaaaggat
     3601 ggcaaaccat cttgttccca ggctccaagc cccgtacaca gcacaatctc tgcctgacgg
     3661 taacggtcaa taaaccagcc gtcgttttgg ttccaaatat aatctactgg cgagttttgg
     3721 taaatagcat catcgttgta gtaatcaccg acaaagaaac gtgcgtcgta aacaccactg
     3781 agagcaatca ctttggtaaa gacatctgga tgctggagga agaaattgag tgcatgatag
     3841 gctcccatag agcaacctgt cgtcatcatg ccatcaaacc aacctgtctt gtgcttgata
     3901 aaagaatggc ctcctcaatc acataacatt cgtaggcacg gtgcatttcc gcttggtcat
     3961 gagcattttt ccaagtagcc aaccagctct cactatccaa actagatagg gtaaagaact
     4021 ggacacggcc ttcctcgata aaggaagcac aggcatcaat catgccaaaa tcatagtatt
     4081 cgttgtgact actacctgat gaagcaaaga ccacaactgg aatcccacca tgtccataac
     4141 ggttaaggta catttcacgg ttaggatgac cactccagtg gctaagatgt tcaatatgca
     4201 tttcgttttt tcctttctta acttaccatt tttctgcaaa aaatctcaga caatctggta
     4261 gattttctga ccaagggatt tcactatgga tggcaccaga ctgaactttt agcacaagat
     4321 tatccagatg tactccccct gctatcaaat catggtaata gcaaagcgac gagtcgatat
     4381 aggcttgttt gatattgcca tccatcaagg tcttgtctgt atcatctgct tcttctgttc
     4441 ctacatagat gaagatgcgc tggtcaggcg atagtttctg gcactcgata tagcggttaa
     4501 aggcttcttg gtggagccag tttgcagatg aaaaaacgcc caagcaacca atttggtctt
     4561 ggtattccaa accgataaac tgggtaatat tgcctcctag tgaggaacca atcatagccg
     4621 tatgctggca gtctgctttt gtacgatagg tctcatcgat aaaaggcttg accacctcca
     4681 tgacaaactc agcatactcc acacccttac caccaaactg ctgctctggg ataggaaatt
     4741 cttggaactt ccaagccgca tactcattca ttcgccccat accatcattg tcaatagcaa
     4801 cgacaatcat gcgactgata tactgatatc cggatttcgt ttgatagctg ggataatctt
     4861 ccatgaatat ccaatgaaag actctttgct ataaaaaaca ttttgcccgt catgaaagta
     4921 tacaacagga taggaacggt ctgtatcttt ctcataatct ttaggaagaa gaatacgtac
     4981 acggcgctcc ttacctgtat aaggaacctt gagtttgtgt tctttcattt ttagataaaa
     5041 gtaggattga ttcattgtca gaaaactctc tatattcaaa attttatatc attatatcat
     5101 gaaaatagaa aaaagctagt tttttacctg tttttagaaa aaaaactaac tttttttact
     5161 gattttcgct attcttttgg attttctgaa ttagagtaga ttatcaatca agtcatcaac
     5221 ttcatctttt tcggcttgag gtgtgatttc agtaatcatg attccagcca ctgctcgctc
     5281 aaccaagcct tcaacatcca tacgcgcttc gtactgctcc gcattcttaa ttttaggatt
     5341 tgtttttgga cgatctggtg caaaaatggt acaacagtct tcaaacggtt ggattgaaat
     5401 gtcaaaggta tcgatttcct gggcgatgtc aatgatttcc aacttgtcca tggtaaccac
     5461 aggacgaatg atgggagtgt tggtaacagc attgatagcc ttcatacttt caagggtttg
     5521 gctggctact tgacctagac tttccccatt gatgataacc aaaccatttc gtacctcacg
     5581 aatacggtca gtaatacgca tcataaaacg acgcgtcaag gtcataaggt aggcttctgg
     5641 cgctttggct ttgatttcct cttgaatctc tgtaaaagga acttcgataa actggatatt
     5701 tcccccaaat ttggttaatt tacgggtcaa atcttgggct tttttaagag caccaggact
     5761 cgtgtaaggt gggctggcaa agtgaactgc ctctatatct acccctcgtt taagcgctag
     5821 atagccagct acaggggaat cgattccccc tgacaacatg agcatgccct taccagaagt
     5881 tcccactggt aatccacctg cccctcgaac ggtttcataa gaaagatagg ctgcttcttc
     5941 acgtatctcc acctgaagat tgatgtcagg acttttcatt tgagcttgta cgtttggaat
     6001 ggcttcgaat acagcccctc caagcgttcg gttgagttca cgactgtcca attcaaaggt
     6061 gtggtcgcta cgcttgctag agattttaaa ggtcatgcct tccttgtaga tgtcctgcat
     6121 aatctcttgg acagcagact tcagaacttc tacagatttt tcaaccttat aaacagggga
     6181 aaagttttga attccaaata cttgtttgag cgattctgct actgctgtgt aatctgctcc
     6241 attgaggtaa gcgtgggcac ggtcgcgatc tgctgttacc ttaacttggg gatagataga
     6301 caaaacgtct gaaatattat tgcgaagttt attgatgaaa cgcatacgat ttttgccctt
     6361 ggttgacagc tcaccgtagc gaatcataat ttctgaatac tgcatgaatg ctcctatctt
     6421 acttttctag tttgattgta aattaatttt agcttggtca agaactgctc gacctgactc
     6481 atatcatttt caaggtctag actaagacgc acagctgact gagccttatc cttgtccact
     6541 cccatagcaa tcagggttcc ggctggtttc ccagccttgg acgaacaagc cgaggtcgtt
     6601 gagatgaaaa tatcatagtc ttcaaaggcg tgaacaatga cctcaccacg aacacccttg
     6661 attccaaaag tcagaatatg aggggcgaag tcttcctcat ctgaaaagac aaatatatcc
     6721 ggatagtcca gaagtgcttg acgaatcact gccttcatct gcccagtctt gctcctaaag
     6781 atatctagct tttccataga taaacggaga gccttagcgg tcgctgcaat tcctgccaca
     6841 ttttcagttg tcgaacggta atcacgttcc tgaccaccac ctgttaaaag aggcgtaatc
     6901 ttcttgccag acttgatata gataaaacca acaccacgga ttccgtggaa cttgtgacca
     6961 gagaaagtcg cgaaatccac tcgttctgtc agatactttt cagtcggaat cttggcaagc
     7021 gcctgaaccg catcaacgtg gaaggaaata gttggcttgt ctgccaatag ttttgaaata
     7081 gcctcaatgg gctgaacaga gccaatttca ttgttaactg ccatgatgga aacgagggtc
     7141 gtatcaggtc ttatcaaatc ttctagcgcc tcaacatcca caaatccttt gttatcaact
     7201 ggagcaaaat ccacttcaaa accttgagct ttcaaccaga gggctgattc cttaactgct
     7261 ggatgttcaa tggctgatac gatgatgtgc ttgccaaact gggctttttc aaatgctact
     7321 cccttgatga tccagttatc cccttctgtc ccaccagatg taaagaagat ttcatcactt
     7381 ttcttaccga ttaaatctgc aatctgttga cgggaagcat ctaaaattcg tgttgcctgg
     7441 tctcccaaac gatggagact agatggattt cctaaaattt ttgaagccac ctgcatatag
     7501 gtttcaaggg cttctggata gggcctggtc gtcgccgaat tatcaaagta aatcatgttt
     7561 ctcacgcttt ctaaaatcac tccttctatt gtatcatgaa acggagcctg cgacaagaaa
     7621 gggcaatgtt tgcattaatc tgaataacat aaaatactct tgataccatc atcaagagca
     7681 ttcttattca ttctatctaa ctttgattat ttctgttaaa aatctaaaga aaagtaattt
     7741 tgtccgcccc agagattaac cgctccccaa ggcgaattta ctcctacacc tatatggact
     7801 gtaccatttg cgcgttggcc atatggaatc gaacttttcc aagtggaacg attacttatc
     7861 gtattaggaa accaagccca gtcagtgaaa tacatatttg acacactgcc actataagta
     7921 tcagaatagc cagaagcatt aaaagtcaca ttaagaacat tcaagcctac caagctataa
     7981 ccgtctaaag aactagactt acttacataa tctgcatgta cttgacttaa aaatgttaac
     8041 catgggatag caaacactac cactattatt tttttcatta tcttatgttt catgatatca
     8101 cctcatccat tctttcggat agtatcgaca actgtttgcc tacaagttgt gtatataggt
     8161 agtaagccac ttattatcag tactgagact gataatccaa ttaaggtgaa aacggaagtt
     8221 agatagaatt taaaaacatc tgaaatttcc aatttaagca cttgcattgc taaaatggaa
     8281 agaatgacga gtaaacaact tgctaatatc gaaattgttt taccataacc aattaattct
     8341 tttaagaaaa gccaaacaat attttgagta ctgtatccca aagattttaa aatccctact
     8401 tcaaagcgtc gaggacgatt gagcaaaaag aaaacaaagg acaatactgc gatgacaagg
     8461 atgagtaaga ctagggatag caacattaaa aattgcctga tataggtgag tcctttatta
     8521 aattcttcgt agttttcata tgatgataaa atagtaattt gtgaagagat tgctcttatt
     8581 ctactcaatt cttcaggaat agcaccgcta tcctttagta acacatgaat cgcactcggc
     8641 atccatgatt tcaaaggaaa accatctaaa gccatcttcg tcatgggatg ctgttgaatt
     8701 gcttcatttt gcaattcttc catttcagca atattcataa atagggtgtt cccataaggg
     8761 gaatattcaa acggataact ttttttaaca atccctaaaa caggatatgt tttatccact
     8821 ttgacaaata aatcgccatc tcctttcaat atattcccct ctttctctat acttgattca
     8881 tataaagaaa ttggaatata aaaagtcagt gtcacggtag ttcccgaaac tatatttggt
     8941 aatttttcat ctgaaatgaa ttgctcagat agataaatcc ctttttcatt gctcctatca
     9001 aaatactcta tctgcctctg tgttgtattt gtctgataca agggttgaat tgaaaaattt
     9061 cttgtcaagc ttctggtctg accatcaata gtcaaagttg gctgaaagtt cttttgaagt
     9121 tgtgaatagt cttttgaatt atcaaacgtc atacccaaag agagaaactc tatataagga
     9181 ttgacggttt gaatattctg acttgtgcta attgaggtga tttcctcctt atcaaaactg
     9241 agaaaatcat ccaacacttt tgtagtttgt aaatgaagcg aatcattaat caccaagaag
     9301 ccattttcga gtgagttgtt caccagttgg tgatacttat ctgcaaataa ggactgaaag
     9361 ttaatagagg ttactgctat agcaactaca agagagataa aaaacgctaa taatctaggt
     9421 aaaaaaggcc ttctatgttt caaaacggat agcttcgtat gagaagacga caaacaggag
     9481 gaccacttct tagaatcctt tatcccttca tcagaattca aaataagaca attcccaact
     9541 aatttatggt ctttaaaatg aagctgaaca tctcctattg caatcatcgc ctcatcatgc
     9601 gaaacaatca atacaatttt atgatatttg tgagctaaat caattaacaa ggcggagact
     9661 ttttctctat tttctttatc caaagaactt gttggttcat cagctaagat gagagtcttt
     9721 cctgtaacta aggctcttgc gattgccact cgttgttctt caccaccgga taaatcttct
     9781 ggataagcat gctttaaaga agaaatctgt aaatccgcca atactgtatc aattttgctt
     9841 tctatttcat ctggactcaa gtcataaaag tttaaagcta gtcttaaatt ttcaaatacg
     9901 gtcaaatcat ttattagatt tcgcccctga aatacaaagg caacttcttc agctataaag
     9961 acagagactg atttcttatt ttcgatatca atctcttttc catcgaagaa ataagaaaca
    10021 ctaggatccg tagcagagag caaaccaatt ttattcagta aagaagactt tcctacgcct
    10081 gacttcccat atattgtata tacttttccc ctttcaaata tcaaatctgt tggctctagc
    10141 aaccgcctgc ctgcaaaaga gagactcaaa tttctaattt cgaacatata acgccctcct
    10201 tttttgaaat cgct
//
LOCUS       DAVKQL010000073         9895 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVKQL010000073 DAVKQL010000000
VERSION     DAVKQL010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9895
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.073"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            126..5066
                     /locus_tag="VW561_001849"
     CDS             126..5066
                     /locus_tag="VW561_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEU9944069.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHTRENKLEKAEGVATASETAEAASAAKPEEK
                     ASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEKEVQPDVPKNTEKT
                     LKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNEKASKEAKVQALSN
                     TNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDAGHRNGVPVYGTLF
                     FNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFINQETTGDLVKPLG
                     EKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYNYQFMQPEGDKVPA
                     DNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKTKVKWNDILDENGK
                     LRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGDKDWYGIANLVADR
                     TPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWRWWQTSTGEKLRAE
                     YDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVAHKGGKGSKVYMAF
                     STTPDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFFEHEGAVKDYQFNL
                     GQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADFYEVYEKDGDSWKL
                     LTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTTFDWGMTVKDTSLP
                     KPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSAQLSGSVDIRLTKP
                     RTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEVRGNKAHVTDITLD
                     KPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMAKAAARSLGNNKVQ
                     VGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQSGLLYYRTQLPGK
                     EISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLRVQYEGGTEDELIR
                     LTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEASPKTILGIEVSQEP
                     KKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQKTGRQTLTLHYQGH
                     EVSFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASSEVKEAFLKAIEAAEQVLK
                     DHETSTQDQVNDRLNKLTEAHKALNGQEKFTEEKTELDRLTGEVQELLAAKPNHPSGS
                     ALAPLLEKNKALVEKVDLSPEELTTAKQSLKDLVALLKEDKPAVFSDSKTGVEVHFSN
                     KEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVDLSYASIVKIPIEK
                     DKKVKKVFFLPEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESAEKPQPAKPAPQNT
                     VLPKPTYQPTSDQQKAPKLEVQEEKVAFHRQEHENTEMLVGEQRVIIQGRDGLLRHVF
                     EVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQNTAVKSEEASKQL
                     PNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            5156..6352
                     /locus_tag="VW561_001850"
     CDS             5156..6352
                     /locus_tag="VW561_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU9944070.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            6488..7015
                     /locus_tag="VW561_001851"
     CDS             6488..7015
                     /locus_tag="VW561_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU9944071.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETISYVFVRVSETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            7092..7448
                     /gene="glnR"
                     /locus_tag="VW561_001852"
     CDS             7092..7448
                     /gene="glnR"
                     /locus_tag="VW561_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEU9944072.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            7485..8831
                     /gene="glnA"
                     /locus_tag="VW561_001853"
     CDS             7485..8831
                     /gene="glnA"
                     /locus_tag="VW561_001853"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEU9944073.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDTEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(8927..9046)
                     /locus_tag="VW561_001854"
     CDS             complement(8927..9046)
                     /locus_tag="VW561_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEU9944074.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            9304..9441
                     /locus_tag="VW561_001855"
     CDS             9304..9441
                     /locus_tag="VW561_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944075.1"
                     /translation="MNPDRAEEYFCRGCQGENPEDIEFYDEQLQAEKVEVLNIRLEVK
                     N"
     gene            9534..9895
                     /locus_tag="VW561_001856"
                     /pseudo
     CDS             9534..9895
                     /locus_tag="VW561_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         3200 a         1789 c         2151 g         2755 t
ORIGIN      
        1 ctattgtttt ttgtctaaaa atgttttagg ataaaaatgt gattatcatg attctagagt
       61 taaaaaacta cagaatataa agcgctttct tgaaaacaac aaaatcaatc ttttaggagg
      121 agaaaatgaa gaatccattt tttgaaagac gttgtcgtta cagtattcgt aagttatcag
      181 taggagcctg ctcgctgatg attggtgctg ttttatttgc tggtccagcc ttggctgaag
      241 aaactgcagt tcctgaaaat agcggagcta atacagagct tgtttcagga gagagtgagc
      301 attcgaccaa tgaagctgat aagcagaatg aaggggaaca tactagagaa aacaagctag
      361 aaaaggcaga aggagtagcg acagcatctg aaactgcaga agcagctagc gcagctaaac
      421 cagaggaaaa agcaagtgag gtggttgcag aaacaccatc tgcagaagca aaacctaagt
      481 ctgacaagga aacagaagca aagcccgaag caactaacca aggggatgag tctaagccag
      541 cagccgaagc taataagact gaaaaagaag tccagccaga tgtccctaaa aatacagaaa
      601 aaacattaaa accaaaggaa atcaaattta attcttggga agaattgtta aaatgggaac
      661 caggtgctcg tgaagatgat gctattaacc gcggatctgt tgtcctcgct tcacgtcgga
      721 caggtcattt agtcaatgaa aaagctagca aggaagcaaa agttcaagcc ttatcaaaca
      781 ccaattctaa agcaaaagac catgcttctg ttggtggaga agagttcaag gcctatgctt
      841 ttgactattg gcaatatcta gattcaatgg tcttctggga aggtctcgta ccaactcctg
      901 acgttattga tgcaggtcac cgtaacgggg ttcctgtata cggtacactc ttcttcaact
      961 ggtctaatag tattgcagat caagaaagat ttgctgaagc tttgaagcaa gacgcagatg
     1021 gtagcttccc aattgcccgt aaattggtag acatggcaaa gtattatggc tatgatggct
     1081 atttcatcaa ccaagaaaca actggagatt tggttaaacc tcttggagaa aagatgcgcc
     1141 agtttatgct ctatagcaag gaatatgctg ctaaggtaaa ccatccaatc aagtattctt
     1201 ggtacgatgc catgacctat aactatggac gttatcatca agatggtttg ggagaataca
     1261 actaccaatt catgcaacca gaaggagata aggttccggc agataacttc tttgctaact
     1321 ttaactggga taaggctaaa aatgattaca ctattgcaac tgccaactgg attggtcgta
     1381 atccttatga tgtatttgca ggtttggaat tgcaacaggg tggttcctac aagacaaagg
     1441 ttaagtggaa tgacatttta gacgaaaatg ggaaattgcg cctttctctt ggtttatttg
     1501 ccccagatac cattacaagt ttaggaaaaa ctggtgaaga ttatcataaa aatgaagata
     1561 tcttctttac aggttatcaa ggagacccta ctggccaaaa accaggtgac aaagattggt
     1621 atggtattgc taacctagtt gcggaccgta cgccagcggt aggtaatact tttactactt
     1681 cttttaatac aggtcatggt aaaaaatggt tcgtagatgg taaggtttct aaggattctg
     1741 agtggaatta tcgttcagta tcaggtgttc ttccaacatg gcgctggtgg cagacttcaa
     1801 caggggaaaa acttcgtgca gaatatgatt ttacagatgc ctataatggc ggaaattccc
     1861 ttaaattctc tggtgatgta gccggtaaga cagatcagga tgtgagactt tattctacta
     1921 agttagaagt aactgagaag accaaacttc gtgttgccca caagggagga aaaggttcta
     1981 aagtttatat ggcattctct acaactccag actacaaatt cgatgatgca gatgcatgga
     2041 aagagctaac cctttctgac aactggacaa atgaagaatt tgatctcagc tcactagcag
     2101 gtaaaaccat ctatgcagtc aaactatttt tcgagcatga aggtgctgta aaagattatc
     2161 agttcaacct aggacaatta actatctcgg acaatcacca agagccacaa tcgccgacaa
     2221 gcttttctgt agtgaaacaa tctcttaaaa atgcccaaga agcggaagca gttgtgcaat
     2281 ttaaaggcaa caaggatgca gatttctatg aagtttatga aaaagatgga gacagctgga
     2341 aattactaac tggctcatct tctacaacta tttatctacc aaaagttagc cgctcagcaa
     2401 gtgctcaggg tacaactcaa gaactgaagg ttgtagcagt cggtaaaaat ggagttcgtt
     2461 cagaagctgc aaccacaacc tttgattggg gtatgactgt aaaagatacc agcctaccaa
     2521 aaccgctagc tgaaaatatc gttccaggtg caacagttat tgatagtact ttccctaaga
     2581 ctgaaggtgg agaaggtatt gaaggtatgt tgaacggtac cattactagc ttgtcagata
     2641 aatggtcttc agctcagttg agtggtagtg tggatattcg tttgaccaag ccacgtaccg
     2701 ttgttagatg ggtcatggat catgcagggg ctggtggtga gtctgttaac gatggcttga
     2761 tgaacaccaa agactttgac ctttattata aagatgcaga tggtgagtgg aagctagcta
     2821 aggaagtccg tggtaacaaa gcacacgtga cagatatcac tcttgataaa ccaatcactg
     2881 ctcaagactg gcgcttgaat gttgtcactt ctgacaatgg aactccatgg aaggctattc
     2941 gtatctataa ctggaaaatg tatgaaaagc ttgatactga gagtgtcaat attccgatgg
     3001 ccaaggctgc agcccgttct ctaggcaata acaaggtaca agttggcttt gcagatgtac
     3061 cggctggagc aactattacc gtttatgata atccaaattc tcaaactccg ctcgcaacct
     3121 tgaagagcga agttggagga gacctagcaa gtgcaccatt ggatttgaca aatcaatctg
     3181 gtcttcttta ttatcgtacc cagttgccag gcaaggaaat tagtaatgtc ctagcagttt
     3241 ccgttccaaa agatgacaga agaatcaagt cagtcagcct agaaacagga cctaagaaaa
     3301 caagctacgc cgaaggggag gatttggacc ttagaggtgg tgttcttcga gttcagtatg
     3361 aaggaggaac tgaggacgaa ctcattcgcc taactcacgc aggtgtatca gtatcaggtt
     3421 ttgatacgca tcataaggga gaacagaatc ttactctcca atatttggga caaccggtaa
     3481 atgctaattt gtcagtgact gtcactggcc aagacgaagc aagtccgaaa actattttgg
     3541 gaattgaagt aagtcaggaa ccgaaaaaag attacctagt tggtgatagc ttagacttgt
     3601 ctgaaggacg ctttgcagtg gcttatagca atgacaccat ggaagaacat tcctttactg
     3661 atgagggagt tgaaatttct ggttacgatg ctcaaaagac tggtcgtcaa accttgacgc
     3721 ttcattacca aggccatgaa gttagctttg atgttttggt atctccaaaa gcagcattga
     3781 acgatgagta cctcaaacaa aaattagcag aagttgaagc tgctaagaac aaggtggtct
     3841 ataactttgc ttcatcagaa gtaaaagaag ccttcttgaa agcaattgaa gcggccgaac
     3901 aagtgttgaa agaccatgaa actagcaccc aagatcaagt caatgaccga cttaataaat
     3961 tgacagaagc tcataaagct ctgaatggtc aagagaaatt tacggaagaa aagacagagc
     4021 ttgatcgctt aacaggtgag gttcaagaac tcttggctgc caaaccaaac catccttcag
     4081 gttctgccct agctccgctt cttgagaaaa acaaggcctt ggttgaaaaa gtagatttga
     4141 gtccagaaga gcttacaaca gcgaaacaga gtctaaaaga tctggttgct ttattgaaag
     4201 aagacaagcc agcagtcttt tctgatagta aaacaggtgt tgaagtacac ttctcaaata
     4261 aagagaagac tgtcatcaag ggtttgaaag tagagcgtgt tcaagcaagt gctgaagaga
     4321 agaaatactt tgctggagaa gatgctcatg tctttgaaat agaaggtttg gatgaaaaag
     4381 gtcaagatgt tgatctctct tatgcttcta ttgtgaaaat cccaattgaa aaagataaga
     4441 aagttaagaa agtatttttc ttacctgaag gcaaagaggc agtagaattg gcttttgaac
     4501 aaacggatag tcatgttatc tttacagcac ctcactttac tcattatgcc tttgtttatg
     4561 aatctgctga aaaaccacaa cctgctaaac cagcaccaca aaacacagtc cttccaaaac
     4621 ctacttatca accgacttct gatcaacaaa aggctcctaa attggaagtt caagaggaaa
     4681 aggttgcctt tcatcgtcaa gagcatgaaa atactgagat gctagttggg gaacaacgag
     4741 tcatcataca gggacgagat ggactgttaa gacatgtctt tgaagttgat gaaaacggtc
     4801 agcgtcgtct tcgttcaaca gaagtcatcc aagaagcgat tccagaaatt gttgaaattg
     4861 gaacaaaagt aaaaacagta ccagcagtag tagctacaca ggaaaaacca gctcaaaata
     4921 cagcagttaa atcagaagaa gcaagcaaac aattgccaaa tacaggaaca gctgatgcta
     4981 atgaagccct aatagcaggc ttagccagcc ttggtcttgc tagtttagcc ttgaccttga
     5041 gacggaaaag agaagataaa gattaaatat cgaaaaatct tgtgaaatct ttccttatat
     5101 ttccaaagtg tgatataata gtttcgaata aaataaataa aggagttttt gtaacatggc
     5161 aaaacttact gttaaagacg ttgacttgaa aggtaaaaaa gtcctcgttc gtgttgactt
     5221 caacgtacca ttgaaagatg gcgtaatcac taacgataac cgtatcacag cagctcttcc
     5281 aactattaag tacatcatcg aacaaggtgg acgtgcaatt cttttctctc accttggacg
     5341 tgtgaaagaa gaagctgata aagctggtaa atcacttgct cctgtagcag ctgacttggc
     5401 agcaaaactt ggtcaagatg ttgttttccc aggtgtcact cgtggtgctg aattagaagc
     5461 ggcaatcaac gctcttgaag atggacaagt tctcttggtt gaaaacactc gttacgaaga
     5521 tgttgacggc aagaaagaat ctaaaaacga tcctgaactt ggtaaatact gggcatcact
     5581 tggagatggt atcttcgtaa acgatgcatt cggtacagct caccgtgcac acgcatctaa
     5641 tgttggtatc tcagcaaacg ttgaaaaagc agttgctggt ttccttcttg aaaacgaaat
     5701 tgcctacatc caagaagcag ttgaaactcc agaacgtcca ttcgtggcta tccttggtgg
     5761 ttcaaaagtt tcagacaaga tcggtgttat cgaaaacttg cttgaaaaag ctgataaagt
     5821 ccttatcggt ggtgggatga cttacacatt ctacaaagca caaggtatcg aaatcggtaa
     5881 ctcacttgta gaagaagaca aattggatgt tgcgaaagct cttcttgaaa aagcaaatgg
     5941 taaattgatc ttgccagttg actcaaaaga agctaacgca tttgctggtt acactgaagt
     6001 gcgtgacact gaaggtgaag cagtttctga aggcttcctt ggtcttgaca tcggtccaaa
     6061 atctatcgcc aaatttgacg aagctttgac tggtgccaaa acagttgtat ggaacggacc
     6121 tatgggtgta tttgaaaacc cagatttcca agctggtaca atcggtgtga tggacgctat
     6181 cgtgaaacaa ccaggagtta aatcaatcat cggtggtggt gactcagctg ccgcagcgat
     6241 taaccttggc cgtgcagaca agttctcatg gattagtacg ggtggtggag catcaatgga
     6301 acttcttgaa ggtaaggttc ttccaggact tgcagccttg acagaaaaat aagattttat
     6361 aaataaatca aagaagagag ggatgaaagt tcctcttttc ttttgcttaa aataaaaacg
     6421 cttcctctca actattactc ataaaaatca ccgatttatg ataaaatgga aatagaaagt
     6481 tgagattatg agttatttta aaaaatataa attcgataaa tcccagttca aacttggtat
     6541 gagaaccttt aaaacaggta ttgctgtttt tctagttctc ttgatttttg gcttttttgg
     6601 ctggaaaggt cttcaaattg gtgctttgac agccgttttt agcctgaggg agagttttga
     6661 tgagagtgtt cattttggga cttcgcgtat tctaggaaat agtatcggtg gactctatgc
     6721 cttggtcttc ttcttattaa ataccttttt ccatgaagcc ttttgggtga ccttggtagt
     6781 tgttccaatc tgcaccatgt taaccattat gacaaatgta gccatgaata acaaagcagg
     6841 ggttattggt ggtgtagcag ctatgttaat cattacccta tcaattccaa gtggtgagac
     6901 aatttcgtac gtgtttgtgc gtgtatcaga aacgtttatg ggagtttttg tcgcaattat
     6961 cgtaaattac gatattgatc gtattcgtct ctttttagag aaaaaagaaa aataatgtta
     7021 cattttataa cattatcaat tgacgtttgt ctttttttag actataatag acagaaagaa
     7081 ggaaattgta aatgaaggaa aaagaatttc gccgaaatat ggctgttttt cctatcggca
     7141 gtgttatgaa gttgaccgat ctatcggcgc gtcagattcg ttattatgaa gatcaagagt
     7201 tgatcaagcc cgatcgaaac gaaggaaatc gtcgcatgta ttccttgaat gacatggatc
     7261 gtctgcttga aatcaaagat tatatctctg aaggttataa tatcgctgcc attaagaaaa
     7321 aatatgctga acgtgaagcg aaatccaaga aagcggttag tcagactgaa gtacgtcgtg
     7381 cacttcacaa tgaactcctc caacaggggc gttttgcttc agtacagtca ccttttggtc
     7441 gcggttaggc aaccgcaagt agtcatagat taaggagaaa acttatgcca atcacagctg
     7501 cagatattcg tcgtgaagtc aaggaaaaaa atgttacctt tattcgtctt atgttctcag
     7561 atattttggg aaccatgaaa aacgtcgaaa ttcctgctac agatgaacag ttagataagg
     7621 tcttgtcgaa caaggttatg tttgatggat cttctattga aggttttgta cgtatcaatg
     7681 agtcggatat gtacttgtat ccggacttgg atacatggac agtcttccct tggggagatg
     7741 aaaatggaag tgttgcaggt ctgatctgtg atgtctatac aacagaaggt gaaccatttg
     7801 cgggtgaccc tcgtggtaat ttgaaacgag ctcttcgtca catggaagaa gttggattca
     7861 aatccttcaa ccttggtcca gagccagaat tcttcctatt taagttggat gaaaatgggg
     7921 acccaacact tgaagtgaat gacaagggtg gctactttga tttggcacct actgaccttg
     7981 cggacaacac acgtcgtgag attgtgaatg tcttgaccaa aatgggattt gaagtagaag
     8041 cgagtcacca cgaggttgcg gttggacagc atgagattga ctttaagtac gatgaagttc
     8101 tccgtgcttg tgataagatt caaatcttta agcttgttgt taaaaccatt gctcgcaaac
     8161 acggacttta cgcaacattt atggcgaagc caaaatttgg tattgctgga tcaggtatgc
     8221 actgtaatat gtccttgttt gatacagaag gaaataacgc cttctttgat ccaaatgatc
     8281 caaaaggaat gcagttgtca gaaacagctt accatttcct aggtggtttg atcaagcatg
     8341 cttacaacta tactgccatc atgaacccaa cagttaactc atacaaacgt ttggttccag
     8401 gttatgaagc gcctgtttac attgcttggg ctggtcgtaa ccgttcgcca cttgtgcgcg
     8461 tacctgcttc acgtggtatg ggaactcgtc ttgagttgcg ttcagtggat ccaatggcga
     8521 acccttacgt tgctatggct gttcttttgg aagttggttt gtatggtatt gaaaataaaa
     8581 tcgaagcacc agctcctatc gaagaaaata tctacatcat gacagcagaa gagcgcaagg
     8641 aagctggtat tacagacctt ccatcaactc ttcacaacgc tttgaaagct ttgacagaag
     8701 atgaagtggt taaagctgct ctcggagatc acatctatac tagcttcctt gaagccaaac
     8761 gaatcgaatg ggcaagttat gcaaccttcg tttcacaatg ggaaattgat aattatttag
     8821 acctttacta atactaatat agaagaaaga ttgcctgtgg gtaaacagtc acacagcagt
     8881 ctaagccatc aaatacctat aacccttaaa atagcgattt cggtcgctat ttatttttcg
     8941 aaaaatcctc ttgactcttt tgcgaattag atttatcatc agacgtgtag aagaaaatta
     9001 aggagtcact atggaaaaat tcaatttcaa aaacaatatc gggcaagaaa acaagttact
     9061 tcaaatcgaa atctacaagt ttactaactt ttgcaagctt caaaattaca catctgtaaa
     9121 tattttttcg aaagatatat ttgaagccat agtaaattga gactagaata gtacactaca
     9181 tctgctaaaa atttctagaa actaatttaa ctttcctaat cgatttgttt acatcttatt
     9241 tcaatctact atattatctc aataaatgat gatggcgact gttttgtttg tgcttgtcga
     9301 tttttgaatc ctgatcgagc ggaagaatat ttctgccgag gttgccaagg ggaaaatcct
     9361 gaagatattg aattttatga tgaacaatta caggcagaaa aggtggaggt tttgaatatt
     9421 cgattggaag taaaaaattg aattatctac aatctcatca acaataaacc tatttcaacc
     9481 ccattatcta taggcgtatt tttacgcctt tttatatact tgaagcacat aagaaacatt
     9541 atgggaagtt tgctgatgga agcactcaag aaattgatgt tccttatgat attcctgata
     9601 cttgggagtg ggtgaggata aaatcaattt attggaattt tgggcaaaat aagccagaga
     9661 aatcctttag gtatatagat acgtctagta ttgatagaaa aaagaatata atcaactaca
     9721 aaaatctaca atatctttca cctgaacaag cgccttcccg tgccagaaaa ttagtttcgc
     9781 agaatagtgt cttattttca acagttagac catatctaaa aaatattgct gtagttagag
     9841 aacttaaaga gtatttgata gctagtacag catttattgt tttggatact ttact
//
LOCUS       DAVKQL010000074         9810 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVKQL010000074 DAVKQL010000000
VERSION     DAVKQL010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9810
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.074"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            <1..219
                     /locus_tag="VW561_001857"
     CDS             <1..219
                     /locus_tag="VW561_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9944076.1"
                     /translation="NSKPQADDKKPSTTNNLSKDKQPSNQASTNEKATNKPKKSLPST
                     GSISNLALEIAGLLTLAGATILAKKRMK"
     gene            complement(438..1418)
                     /gene="dusB"
                     /locus_tag="VW561_001858"
     CDS             complement(438..1418)
                     /gene="dusB"
                     /locus_tag="VW561_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEU9944077.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKV"
     gene            complement(1405..2277)
                     /gene="hslO"
                     /locus_tag="VW561_001859"
     CDS             complement(1405..2277)
                     /gene="hslO"
                     /locus_tag="VW561_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEU9944078.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            2442..3869
                     /locus_tag="VW561_001860"
     CDS             2442..3869
                     /locus_tag="VW561_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9944079.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELTIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLFY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSVGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLVAIRQLLFT"
     gene            4028..5536
                     /gene="glpK"
                     /locus_tag="VW561_001861"
     CDS             4028..5536
                     /gene="glpK"
                     /locus_tag="VW561_001861"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEU9944080.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYIVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEIDD"
     gene            5683..7509
                     /gene="glpO"
                     /locus_tag="VW561_001862"
     CDS             5683..7509
                     /gene="glpO"
                     /locus_tag="VW561_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEU9944081.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLVDTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            7579..8283
                     /locus_tag="VW561_001863"
     CDS             7579..8283
                     /locus_tag="VW561_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU9944082.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGGLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            8580..8783
                     /locus_tag="VW561_001864"
     CDS             8580..8783
                     /locus_tag="VW561_001864"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944083.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEDKKQELLNRLNK"
     gene            complement(8870..9457)
                     /locus_tag="VW561_001865"
     CDS             complement(8870..9457)
                     /locus_tag="VW561_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944084.1"
                     /translation="MFVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNILSDEVLE
                     VVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVNTIHGNKQ
                     ANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSPYPYYITS
                     TYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
BASE COUNT         2856 a         1934 c         2219 g         2801 t
ORIGIN      
        1 aatagcaagc cacaagcaga tgataagaag ccatcaacta caaataattt aagcaaggac
       61 aagcaacctt ctaaccaagc ttcaacaaac gaaaaagcaa caaataaacc gaagaagtca
      121 ttgccatcaa ctggatctat ttcaaatcta gcacttgaaa ttgcaggtct tcttaccttg
      181 gcgggggcaa ccattcttgc taagaaaaga atgaaatagt gattgattca ttcaaaaatg
      241 tttgtaaaaa gtattcggct taaaagaaaa gagtctggga taaaaagatt ttgattttag
      301 gaattcttta ttataaattt ttaaaatcga tagattagac aaaaaagcga acaagacaga
      361 attctgagtg tcagataact gattttgttc gctttttata tttaatatta tacttttgtc
      421 ctaggctctt ttttgcatta tactttttcc aattgcaaga gggcttcaat ctctgccagg
      481 gtgctagctt gcgaaatggc tccacggagt ttggcagcgc cagatgttcc ccggagatag
      541 tgaggagcga ggccgcggaa ttcacgaact gcgacgtttt ctcctttgag gttaatcaat
      601 cgtttcaagt gttcgtaggc gatcttcatc ttgtcttcaa aggtcaaatc aggtaggatt
      661 tctcctgttt caaagtaatg gttgatttgg ttgaagaggt aaggatttcc catggcagct
      721 cggccaatca tgactgcgtc agcaccaact tcttcgatgc gttgcttggc ttcttggaca
      781 gtacggatat caccgttggc gatgaatgga atcttggtta gagcttgggc aaccttgtaa
      841 agggtctcaa ggtctgcgtg gccagtatac atttgttcac gggtacggcc atgcatggcg
      901 agggcagaaa cacctgcagc ctcagcagcg agggcatttt ctactgcaag agatgggtcc
      961 gcccagccgg tacgcatttt gacagtaagt gggatatcaa ggacagactg gaccttgttg
     1021 atgatagagt agatcttgtc aggatccttg agccacatag caccagcttc gttcttcacg
     1081 attttgttga cagggcagcc catgttgata tcgacgatat cggtcttggt gttttcttgg
     1141 atgaattctg ctgcgcgtgc taggctgtct tcatcgctac caaaaagttg gatagagaca
     1201 gggttttcgc cctcatcgat atgaagcatg tgcagggttt tttcgttgtt gtattggatt
     1261 cccttgtcag agaccatttc cattacaacg agtccagctc cgagctcttt tgcgatggta
     1321 cgaaaggctg agttggtcac gccagccata ggcgctaaaa cggtacgatt gggaatctca
     1381 acattgccaa tcataaaagg tgtattaaga tttgtcacga atgagttcct ccaggtcctt
     1441 ttcatcaaag ttgtaagtag tttggcagaa ttgacaagtg atttctgccc cgtggtcttc
     1501 ctctttcatt tcctgtaagt ctgagcttgg aaggctggca agagcgttca taaagcgttc
     1561 atggctacag tcacattgga aacggatttc ttcttcagaa agacgcttgt aggcttcgtc
     1621 cccgtagata gccttgagga gggcttcgat atggtcgtcg ctttcgagaa gagtagagat
     1681 agctggcatt tcttggatgc gtttttcaaa gcgagcaatc tcttctttct tggctcctgg
     1741 caagacttga actaggaaac cacctgcaac cttgaccttg tcttcctcgt ccaaaaggac
     1801 attgaggccg accgctgaag gcgtttgttg gctttcagta aggtaaaagg caaggtcttc
     1861 accgatttct ccagagatga ggggagttat agagttgtaa ggatttccag taccgtagtc
     1921 tgtgataacg aggaattgac catttccaac aaaaggtccg actaggactt caccagtcgc
     1981 agtctttttg atgtcaacac caggattttg aacatagcct ttgacgttcc ccttggtatc
     2041 agcgacggtg ataatagcac ctagagagct agagcccaac accttaactg taagtttggt
     2101 atttcctttt tcattggctg cgagaatctg gctggcgata agagttcgac caagcgctac
     2161 agttgagcta gcttgggttt gatgtttttc ttgagcagtg cggacggttt ccgtgctatc
     2221 aaggacaaaa gcacgaaagg ctccgctttc tgatatagtt ttaataattt tatccatagc
     2281 tactatttta gcataaaaat gcccaaaggg ggagccgtgt gtttactgat tttcaggata
     2341 atggaccagg aaatcagcat gaaaataaaa agagaaacag attattttag catttgtaag
     2401 atttatgcta tgcttaaggt agaaaatgaa aggggtaaca aatgtattta ggagatttga
     2461 tggagaaagc cgagtgtggt caattttcaa tcctttcctt tctattacaa gagtctcaga
     2521 cgaccgtcaa ggctgtaatg gaagaaacag gattttcaaa agcaacccta accaaatatg
     2581 tcaccctgct caatgacaag gctttggata gtggcttaga actgactatt cactcagaag
     2641 atgaaaatct gcgtctgtct atcggtgcag ctaccaaggg gagagatatt cggagcttgt
     2701 ttttggagag tgctgttaaa taccagattt tggtttatct tttctaccac caacagtttt
     2761 tagcccatca gctggctcaa gaattggtga ttagcgaggc tacgcttggt cgtcacttgg
     2821 ctggtttaaa tcagattttg tcagaatttg atttatccat ccaaaatgga cgttggcgag
     2881 gtccagagca tcagattcgc tatttctatt tctgtctttt ccgaaaggtc tggtcgagtc
     2941 aggaatggga aggtcacatg cagaaaccag agagaaaaca ggagattgcc aatttagagg
     3001 aaatctgcgg tgcaagtttg tctgtggggc agaaattgga cttggttctc tgggctcaca
     3061 tcagtcaaca acgtcttcgg gtcaatgctt gtcagtttca agtcatagaa gagaaaatgc
     3121 gagggtattt tgacaatatc ttctatcttc gtttgctgag aaaggttccg tccttttttg
     3181 ctgggcaaca tattccacta ggagttgagg atggtgagat gatgatattc ttctcttttc
     3241 tcctatctca tcgcattctt cctcttcata ctatggagta tattcttggt tttggagggc
     3301 agttggcaga tttactgacg caattgattc aagaaatgaa gaaggaggaa ctattggggg
     3361 attatacaga ggaccatgtc acctatgaac tcagtcagct ttgtgctcaa gtctatctct
     3421 ataagggcta tattttacag gatcgctaca agtaccagtt agagaatcgt catccatatt
     3481 tactgatgga acatgatttt aaagagacag cagaggagat ttttcatgct ctacctgctt
     3541 ttcaacaggg gacagattta gataagaaga ttctctggga atggctccag ttaatcgaat
     3601 atatggctga aaacggtggt cagcatatgc ggattggtct ggatttgaca tctggttttc
     3661 ttgtcttttc aaggatggca gccattttga aacggtattt ggaatacaat cgttttatta
     3721 ccattgaagc ttatgaccct agtcggcatt atgatttgct ggttaccaat aacccgattc
     3781 ataagaagga acagacacca gtctattatt taaaaaatga cttggatatg gaggatttgg
     3841 tagcgattcg ccagttatta ttcacttaaa aggcttggtt aatccaggtc ttttttgtga
     3901 aattcacaca atctcctcac atttttttaa aaattaaaaa aagttgataa acaagaaagc
     3961 gctttatttt gtatactagt aagtgtaaag aggaaacacc tcaagatctt tatcaggagg
     4021 acagtatatg tcacaagaaa aatacatcat ggccattgac cagggaacta caagttctcg
     4081 tgccatcatt ttcaacaaaa aaggggaaaa ggttagctcg agtcaaaaag agtttactca
     4141 gattttccca caagcaggtt gggttgagca caatgccaat gaaatttgga actcagtaca
     4201 gtcagttatt gcgggtgctt tcatcgaaag tggtgtcaag ccaaatcaaa tcgaggcaat
     4261 cgggattact aaccagcgtg aaacaacggt tgtctgggat aagaaaacag gacttcctat
     4321 ctacaatgct atcgtttggc agtcacgcca gacagcacct ttggctgagc aactaaaaag
     4381 ccaaggttat gtggaaaaat tccatgaaaa gactggtttg attattgatg cttacttctc
     4441 tgctaccaag gttcgttgga ttttggatca tgtagaaggt gctcaagagc gagcagaaaa
     4501 aggggaattg ctctttggta ctatcgatac ttggttggtt tggaaattga ctgacggtgc
     4561 ggctcacgtg actgactact caaatgcagc tcgtaccatg ctttataaca ttaaagaact
     4621 caaatgggat gatgagattt tggaaatcct taacattccg aaggctatac ttccagaagt
     4681 tcgttctaac tccgaaatct acggcaagac agctccattc catttctacg gtggagaggt
     4741 gccaatctca ggtatggctg gggaccaaca agcagccctc tttggacagt tggcttttga
     4801 gccaggtatg gttaagaata cttatggaac aggctctttc atcatcatga atactgggga
     4861 agagatgcag ttgtctgaaa acaacctctt gacaaccatt ggttacggaa tcaacggtaa
     4921 ggtttattat gccttggaag gttctatctt catcgcagga agtgctattc agtggcttcg
     4981 tgacggtctt cgcatggttg aaaattcacc agaatctgaa aaatacgctc gtgattctca
     5041 caacaacgat gaagtttata tcgttccagc ctttacaggt ctaggcgctc catactggaa
     5101 ccaaaatgct cgtggttccg tctttggttt gactcgtgga acaagcaaag aagactttat
     5161 caaggcgact ttgcaatcta ttgcttatca agtgcgtgac atcatcgaca ccatgcaagt
     5221 ggatactcag accgccattc aagtactgaa ggtggatggt ggtgcagcca tgaacaactt
     5281 cctcatgcag ttccaggcgg atattttagg cattgatatt gcacgtgcta aaaacttgga
     5341 aacaacagct ctaggagcgg ccttcctagc aggtttgtca gtagggtact ggaaagactt
     5401 ggacgagttg aaactcttga acgagacagg agaactcttt gagccatcta tgaacgaatc
     5461 tcgcaaggaa caactctata agggctggaa gaaggctgtg aaagcaactc aagtctttgc
     5521 ggaaatcgat gactaatact caatgaaaat caaagagcaa actaggaagc tagccgcaag
     5581 ctgtacttga gtacggtaag gcgacgctga cgtggtttga agagattttc gaagagtata
     5641 atactggcag aataaagcga tttagttaga aagtgtgtaa atatggaatt ttcaaagaaa
     5701 acacgtgaat tgtcaattaa aaaaatgcag gaacgcaccc tggacctctt gattatcggt
     5761 ggaggaatca caggagctgg tgtagccttg caggcggcag ctagcggtct tgagactggt
     5821 ttgattgaaa tgcaagactt tgcagaagga acatctagtc gttcaacaaa attggttcac
     5881 ggaggacttc gttacctcaa acaatttgac gtagaagtgg tctcagatac ggtttctgaa
     5941 cgtgcagtgg ttcaacaaat cgctccacac attccaaaac cagatccaat gctcttacca
     6001 gtttacgatg aagatggagc aacctttagc ctcttccgtc ttaaagtagc catggacttg
     6061 tacgacctct tggcaggtgt gagcaacaca ccaactgcga acaaggtttt gagtaaggat
     6121 caagtcttgg aacgccagcc aaacttgaag aaggaaggct tggtaggagg tggagtgtat
     6181 cttgacttcc gtaacaacga tgcgcgtctc gtgattgaaa acatcaaacg tgccaaccaa
     6241 gacggtgccc tcattgccaa ccacgtgaag gcagaaggct tcctctttga cgaaagtggc
     6301 aagattacag gtgttgtagc tcgtgatctc ttgacagacc aagtatttga aatcaaggcc
     6361 cgtctggtta ttaatacaac aggtccttgg agtgataaag tacgtaattt gtctaataag
     6421 ggaacgcaat tctcacaaat gcgcccaact aagggagttc acttggtagt ggattcaagc
     6481 aaaatcaagg tttcacagcc agtttacttt gacacaggtt tgggtgacgg tcgtatggtc
     6541 tttgttctcc cacgtgaaaa caagacttac tttggtacaa ctgatacaga ctacacaggt
     6601 gatttggagc atccaaaagt aactcaagaa gatgtagatt atctacttgg cattgtcaac
     6661 aaccgctttc cagaatccaa catcaccatt gatgatatcg aaagcagctg ggcaggtctt
     6721 cgtccattga ttgcagggaa cagtgcctct gactataatg gtggaaataa cggtaccatc
     6781 agtgatgaaa gctttgacaa cttgattgcg actgttgaat cttatctctc caaagaaaaa
     6841 acacgtgaag atgttgagtc tgctgtcagc aagcttgaaa gtagcacatc tgagaaatat
     6901 ttggatccat ctgcagtttc tcgtgggtct agcttggacc gtgatgacaa tggtcttttg
     6961 actcttgctg gtggtaaaat cacagactac cgtaagatgg ctgaaggagc tatggagcgc
     7021 gtggttgaca tcctcaaagc agaatttgac cgtagcttta aattgatcaa ttctaaaact
     7081 taccctgttt caggtggaga attgaaccca gcaaatgtgg attcagaaat cgaagccttt
     7141 gcgcaacttg gagtttcacg tggtttggat agcaaggaag ctcattacct agcaaatctt
     7201 tacggttcaa atgcaccgaa ggtctttgca cttgctcaca gcttggaaca agcgccaggt
     7261 cttagcttgg tagatacttt gtcccttcac tatgcaatgc gcaacgagtt ggctcttagc
     7321 ccagttgact tccttcttcg tcgtaccaac catatgctct ttatgcgtga tagcttggat
     7381 agcatcgttg agccagtttt ggatgaaatg ggacgattct atgactggac agaagaagaa
     7441 aaagcaactt accgtgctga tgtcgaagca gctctcgcta acaacgattt agcagaatta
     7501 aaaaattaag aaaaaataaa agaggtggag ggcagcattc cttgtcgccc gtcccttctt
     7561 tttaatggag acagaaagat gatgaatgaa ttatttggag aatttctagg gactttaatc
     7621 ctgattcttc taggaaatgg tgttgttgca ggtgtggttc ttcctaaaac caagagcaat
     7681 agctcaggtt ggattgtgat tactatgggt tgggggattg cagttgcggt tgcagtcttt
     7741 gtatctggca agctcagtcc agctcattta aacccagctg tgaccatcgg tgtggcctta
     7801 aaaggtggtt tgccttgggc ttccgttttg ccttatatct tagcccagtt cgcaggggcc
     7861 atgctgggtc agattttggt ttggttgcaa ttcaaacctc actatgaggc agaagaaaat
     7921 gcaggcaata tcctggcaac cttcagtact ggaccagcca tcaaggatac tgtatcaaac
     7981 ttgattagcg aaatccttgg aacctttgtt ttggtgttga caatctttgc tttgggtctt
     8041 tacgattttc aggcaggtat cggaaccttt gcagtgggaa ctttgattgt cggtatcggt
     8101 ctatcactag gtgggacaac aggttatgcc ttgaacccag ctcgtgacct tggacctcgt
     8161 atcatgcaca gcatcttgcc aattccaaac aagggagacg gagactggtc ttacgcttgg
     8221 attcctgttg taggccctgt tatcggagca gccttggcag tgcttgtatt ctcacttttc
     8281 tagtttatac tcttcgaaaa tcaaattcaa accacgtcag cgtcgcctta ccgtactcaa
     8341 gtacagcttg cggctagctt cctagtttgc tctttgattt tcattgagta ttagaaaaca
     8401 attatgttga tagagcttgg gcaagagccc aatttcagca aaaaatgaag taaatcttct
     8461 cataataaaa cgcatcatat caagcacgaa aattccacga ggtcaactac agtcagaaag
     8521 ctgaacaaca agccaaaacg cccaaaaaag gcggcaaaaa gcaagcacct gcaagcaacg
     8581 tgccgaaatg gtcaaatcct gattatgtca acgaattaga cccaaaaatc gttgatatgc
     8641 tagtagaatt tcacaagtca caaggcactt tggaaactcc cgaggcgcaa gcagaaatcg
     8701 cccaaaaacg tgaagaaatc gagcaaagga gagctgagct tgaggataaa aaacaagagc
     8761 ttttgaaccg cttgaacaaa tagagtttcg caagtattat gcttacaaat tacttgagca
     8821 attaactaaa atataaaccc tgcctttata tctaggcagg gtttatattt tagaaattca
     8881 cgtaggttgt tacggttttt acatacccag tatagtttga gtttctatag tattcagtga
     8941 taaacttcca ttttctttga gcaacatgga tataagtact tgttatgtag tatggatatg
     9001 ggctttgtga atccaagtaa gactgataag cttgtatacc aaaatatgct ccaccaatta
     9061 ttgcacccca tggacccccc aataaagcac ctatcctacc aatcatataa ctgattccag
     9121 caccagtcat gaagttagcg aatgtgttag cttgtttatt cccatgtatt gtgttgacgt
     9181 aattccaaac attaggatcg tatgatctaa aagatatatt taggtcgatt tcattctttt
     9241 gataagccat ataaaatgcc ccattgatat agacgccgtc agcacgtcgt tcaatagtgt
     9301 ctacacttcc atctggattg acaacctcaa gaacttcatc gcttaaaata tttacttgcg
     9361 tatctccgaa ccgcactgat gagccattct caaactgagc ctcaccagat acaactttag
     9421 agtttgccga taagctatca tcagcaaaaa caaacaagcg acggggaaat gctagacata
     9481 cagaaaacag acataactag caaacacatg catttaaaca tcttagacat aacggaaact
     9541 cctttgtatt tttgattttt ttcaactttt attatacaat aaaaccaaat aaaaagaaag
     9601 cggtaacaat atgcttaatg cgaaaatttt ttatatattt ttatgtttga tcgttatcga
     9661 aactacaggc ttgttgttgt tgaaaagagg tctcgaaatg ggttatttag acacagaagc
     9721 tattatcctc gcagtttttt catttgcttt ttacaaccta tgttcattcg cttgggtctg
     9781 ctctacaata aaaaaacaat aaaaaataaa
//
LOCUS       DAVKQL010000075         9606 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVKQL010000075 DAVKQL010000000
VERSION     DAVKQL010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9606
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.075"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(75..272)
                     /locus_tag="VW561_001866"
     CDS             complement(75..272)
                     /locus_tag="VW561_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU9944085.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSSLSAISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
     gene            complement(347..1123)
                     /locus_tag="VW561_001867"
     CDS             complement(347..1123)
                     /locus_tag="VW561_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEU9944086.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGINALHPEDLIDYQKVFLNHHINCYF"
     gene            1245..1991
                     /locus_tag="VW561_001868"
     CDS             1245..1991
                     /locus_tag="VW561_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9944087.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     HQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            2004..2984
                     /locus_tag="VW561_001869"
     CDS             2004..2984
                     /locus_tag="VW561_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEU9944088.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            3179..3499
                     /locus_tag="VW561_001870"
     CDS             3179..3499
                     /locus_tag="VW561_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9944089.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            3545..3853
                     /locus_tag="VW561_001871"
     CDS             3545..3853
                     /locus_tag="VW561_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9944090.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQSSGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            3870..5168
                     /locus_tag="VW561_001872"
     CDS             3870..5168
                     /locus_tag="VW561_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9944091.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASANLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVVTQLLVLVISTVVYFPFFKIQDRLAYQNEIKQS"
     gene            5319..7757
                     /locus_tag="VW561_001873"
     CDS             5319..7757
                     /locus_tag="VW561_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEU9944092.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            7776..8444
                     /locus_tag="VW561_001874"
     CDS             7776..8444
                     /locus_tag="VW561_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEU9944093.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEVGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVTQN
                     SRSI"
     gene            8462..9550
                     /locus_tag="VW561_001875"
     CDS             8462..9550
                     /locus_tag="VW561_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEU9944094.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
BASE COUNT         3169 a         1343 c         1932 g         3162 t
ORIGIN      
        1 tcctgataga gatgtttaca tcttatttca attcgctata ttagagtaaa attctctaca
       61 aaaagaagaa tagcctattt tactattctt ccgagtgatt tcaatttctt tggggaaata
      121 tggagatact ttttaaatac tttggcaaaa tatttatagt ctgaaaatcc aacttggtca
      181 gagatagcgc ttaaagagga gttgggagat tcagccatct tatctatagc ttgtttcagg
      241 cgatattgta agatatattc atttagagtc atggggaagt cttctttgat aaccttatat
      301 agatgcttgt tatctgcaaa tagctagagc atttcaagga aataaactag aaataacaat
      361 taatatggtg gttcagaaat accttttgat aatcaataag atcttcagga tgaagtgcgt
      421 tgattccatc catttcatat ttccgattta ataaacgata tttgttttca ccaaattgat
      481 gaaaagggag tagttgaact tggtcgatat ttaatgagtt aaatagagta gcgaattttt
      541 ctgcatcctc taaactattg ttaaaattag gaataactgg gattcttaaa acgatagttt
      601 tattttgaga aaaagcataa tgaatgtttt taataatcat ttgattaaaa accccagtca
      661 cttttttatg ttttatagaa ttataatgtt ttaggtctgt gtagataaaa tccacatatt
      721 gaattaaatc gataaatttt tcatgatcaa caaaggcagt agtttcaatg gcagtgtgta
      781 tgtgatgttc tttagctgat tttaagatgg ctttagcaaa ttcaaactga gcaaatattt
      841 cacctcctga taaagttaaa cctccaccgg attcttcgta aaattctttg tcttttaata
      901 cctctgtaat aatttcttct acagtctttt cttcaccgac taaggtgaat ttctctcgtt
      961 gagcatcttt cattttttca ggtttcattc tttgagattc aggattagaa caccatggac
     1021 agcgcagagg acatcctttt aaaaaaacag ttgtacgaat acccgggccg tcatgaattg
     1081 aaaagtgttg aatattaaaa ataattcctt tagatatttc cataagagtt cctcctcttc
     1141 ataagtctat cctatcacaa aaacgaaaga aaaacaatta cgaacgaaag gttaatttgt
     1201 tgttttttgc tttttatttt ggtagaataa aacggaggtg taaaatgaaa agattggaac
     1261 aaattattaa attagtatca gaatatgaaa agatcgacgt taatacatta tcggaaaaat
     1321 taaatgtatc gaaagtaacg attagaaaag atttagataa attagagtca aaaggtttat
     1381 tacacagaga gcatggatat gctgtattaa atagtggaga tgacttaaat gtacgtttgt
     1441 caattaatta tgaaattaag agaaaaattg ttcaggaagc agtaaaattg gtgtcagata
     1501 atgaaacaat aatgatagaa tctggatcga cctgtgcttt acttgctgag gaaatttgca
     1561 agcaaaaaag aaatgttacg attgtaacaa attcattttt tatagcaaat tttgtgagag
     1621 cttatgattc atgtcgtatt attgttcttg gtggagagtt tcagaaggat tcacaggtta
     1681 ctgtgggacc tttattaaaa gaaatgatac agacttttca tgtgcatcaa gcttttgttg
     1741 ggacagatgg ttacgataaa gagatgggct ttacaggaaa agatttaatg cgcagtgagg
     1801 tagttcaata tatttcagca gcgtcggata aagtcattgt cctaactgac tcaagtaaat
     1861 ttgataaaag gggtacagta agaagatttg ctttaagtca agtctatgaa gtaataacag
     1921 acgaaaaact ttctaaacaa aatatagcta cattagaaaa tgctgggata atggttaagg
     1981 tagtttcgta agaggttaag tgtatgaatc aagataggaa taaactgctc tctaaaattg
     2041 cttatctgta ttatattgaa aacttaaatc agtcacaaat agcagcaaaa ttaggaattt
     2101 atagaacctc tattagtaga atgttaacag aagcaaggaa tgcaggaatt gttaaaattg
     2161 aaatagagaa ttttgatacc aatatgttta agttggaaaa ttatgtaaaa gaaaaatatg
     2221 gtctggaaag tttagaaatt attccaaatg aatttgatga taatccaaca attttatctg
     2281 aaagaatttc tcaagttgca gcaggtgtcc ttaggaatct aattgatgat aatatgaaaa
     2341 ttggcttttc ttgggggaaa agtttaagta atttagtaga tttaattcac agtaaaagtg
     2401 tccgaaatgt tcacttctat cctctagcag gtggtcctag tcacatacac gctaaatacc
     2461 atgtgaatac actgatttat gaaatgtcta gaaaatttca tggagagtgt acatttatga
     2521 atgcaacgat tgtgcaagaa aataaattgt tagcagatgg tattttgcaa tcaagatatt
     2581 ttgaaaattt gaaaaatagt tggaaagatt tagatatagc tgtagtcgga attggtgatt
     2641 ttagcaataa aggaaagcat caatggttag acatgcttac agaggatgac tttaaagaat
     2701 tgaccaaagt taaaactgta ggagaaattt gttgtcgatt ttttgattca aaaggtaaag
     2761 aagtctatga aaacttacag gaaagaacga tagcaatctc tttggaagat ttaaaaaata
     2821 ttcctcaaag tttagctgtt gcttacggtg atacgaaagt atcttcgatt ctttctgtct
     2881 tgcgtgctaa tttagtaaat catttgatta cagacaaaaa tacaatttta aaagttttgg
     2941 aagaagatgg ggatttgact tttagagaga ttctaggtga gtgaaaatga tagactgatt
     3001 cagtttatcg tttttctttt tagttgattg cacatttgtg cttatataaa caaaaatagt
     3061 ttatctgttg tttttggatt gacaacttta ttatgtagtt gtattctata gttacaaaag
     3121 aaaattttaa aatttcaaat gaaaaaagct ttttacatag tgaaatgagg aggaatttat
     3181 ggaaattatt gttccagatc aaattatcat gggtttaatt ttatatgctg gcgatgcgaa
     3241 acaacatatt tataaagcat tagattacat aaagaacggt acatgtgaac gatgtgaaga
     3301 agaaatccag ttagctgatg cagccttatt agaagctcat aatctacaaa caaaattctt
     3361 ggcacaggaa gcgtctggta caaagacaga aattacagct ctctttgttc attcacaaga
     3421 tcatctcatg actagtatga cggagattaa tttaatcaaa gaaattatta gtttgagaaa
     3481 agaacttcat aaaaaataat actagagtat tatcattgtt attaacatag aggaggaaaa
     3541 cataatggtg aagattggtt tgttttgtgc agcaggtttt tctactggta tgcttgtaaa
     3601 taatatgaaa attgcagcgc aatctagtgg agttgaggca gaaatagagg cgttttctca
     3661 gtctaaatta gcggattatg cgccaaatat agatgttgca ctattgggtc cacaagttgc
     3721 ttatacatta gataaatcaa aagaaatttg tgataagtgt gatgttccga tagctgttat
     3781 tccgatgatg gactatggta tgttagatgg gaaaaaagta ttagatttgg ccctatcttt
     3841 gattagtggg taagaaaagg agatttatta tgtcaaagat ggatgttcag aaaatcattg
     3901 caccgatgat gaagtttgtg aatatgcgtg gcattatagc tctaaaagat gggatgttag
     3961 caattttgcc attgacagta gttggcagtt tgttcttgat tatgggacaa ttgccgttcg
     4021 aaggattaaa taagagcatt gctagtgttt ttggagctaa ttggacagag ccgtttatgc
     4081 aagtatattc aggaactttt gctattatgg gtctaatttc ttgtttttca attgcctatt
     4141 cttatgctaa gaatagcgga gtagaggctt taccagctgg agttctatct gtatctgcat
     4201 tctttatttt gctaagatca tcttatatcc ctaaacaagg tgaggcgatt ggggacgcta
     4261 ttagtaaagt ttggtttgga ggccaaggaa ttatcggtgc tatcattata ggtttggtag
     4321 taggaagtat ttataccttc tttataaaga gaaaaattgt tattaagatg ccagaacaag
     4381 ttccacaagc tattgccaaa cagtttgaag caatgattcc agcatttgta attttcttat
     4441 cttctatgat tgtatatatt ttagcgaagt cattgactaa tggcggaaca ttcatagaaa
     4501 tgatttattc tgctattcaa gttccgttgc aaggtttaac tggatctttg tatggtgcta
     4561 ttggaattgc attctttata tcatttttgt ggtggtttgg tgttcatggg caatcggtag
     4621 taaatggagt agtgacagct ctgcttttat ctaatcttga cgctaataaa gctatgttag
     4681 cctctgctaa tctatcatta gaaaatggtg cacatattgt tactcaacaa tttttagatt
     4741 catttttaat tctatcaggt tcagggatta cgtttggtct tgtagttgcc atgctttttg
     4801 cagcaaaatc aaaacaatac caagccttag gaaaagttgc agcttttcca gcaatattta
     4861 acgtaaatga gccagttgta tttggatttc cgattgtcat gaatccagtt atgtttgtac
     4921 ctttcattct tgttcctgta cttgcagctg tgatagtata tggagctatt gcaacaggtt
     4981 tcatgcagcc attctcaggg gtaacattgc cttggagtac accagctatt ctatcaggat
     5041 ttttggtggg tggatggcaa ggtgttgtga ctcagttatt agtattggtg atatctacag
     5101 tggtttactt tccattcttt aagatacaag atcgtttagc ttaccaaaat gaaatcaaac
     5161 aatcttagag gtatttgtgt gttactgtta aactcacaca tttgtgctaa aaattagaga
     5221 gttaaaattt ttctagttaa aagcttgaaa atttctataa aaatcggtat tatattttcg
     5281 aaagaaataa aaatattttc gaaagaaagg tgcttacgat ggtaaataca gaagtagcaa
     5341 gaacaacaat caagacagaa tattttggca gccttactga aaggatgaac aaatatcgag
     5401 aagatgtttt aaataaaaaa ccttatattg atgctgagag agcagttcta gcaacacgcg
     5461 cctatgaacg atacaaggaa caacctaatg tcctaaaacg tgcatatatg ctgaaagaaa
     5521 ttttggaaaa tatgtctatc tatattgaag aagaatctat gattgcggga aatcaagctt
     5581 cttccaataa agatgctcct atttttccgg aatatacgct agaatttgtt ctcaatgagt
     5641 tggatctttt tgaaaagcgt gatggagatg ttttctatat tacagaagaa acaaaagaac
     5701 aacttagaag tattgctccg ttttgggaaa ataataattt acgtgctaga gctggtgcct
     5761 tattacctga agaagtgtca gtttatatgg aaacaggatt cttcggtatg gaaggtaaga
     5821 tgaattctgg agatgctcac ttagcagtta actatcagaa acttttgcaa tttggtttaa
     5881 gaggttttga agagcgggct cgtaaagcaa aagtagctct agatttaaca gatccagcaa
     5941 gtattgataa atatcatttt tacgactcta tatttatcgt aatcgatgct attaaagtat
     6001 atgcaaagcg ctttgttgct cttgctaaaa gtttagccga aaatgcaaat cctaaacgta
     6061 agaaagaatt acttgagatt gcagatattt gctctagagt cccatatgaa ccggcaacta
     6121 cttttgcaga agctattcaa tcagtttggt ttattcaatg tattttacaa attgaatcta
     6181 atggccactc tctttcatat ggccgttttg atcaatatat gtatccatat atgaaggctg
     6241 atttagaaag tggtaaagaa acagaagata gcattgttga acgtctgaca aatctttgga
     6301 ttaagacaat tacaattaat aaggttcgca gtcaatcaca tacattttct tcagcaggaa
     6361 gtcctttata tcaaaatgtt acaattggtg gacagactcg agataagaag gatgctgtta
     6421 acccattatc ttatttggta ttaaaatcag ttgcacaaac ccatctaccg caacctaatc
     6481 taactgtacg ttaccatgca ggtttagatg ctcgtttcat gaatgagtgt attgaagtga
     6541 tgaaacttgg ttttggtatg cctgcattta ataatgatga gattattatt ccttctttta
     6601 ttgcaaaagg agtattggaa gatgatgctt atgattacag tgccattgga tgtgttgaaa
     6661 cggcagttcc agggaaatgg ggctatcgtt gcacaggtat gagttatatg aacttcccta
     6721 aggttctact tatcacgatg aatgatggaa ttgatccggc ttcgggtaaa cggtttgcac
     6781 caagctttgg tcattttaag gatatgaaga acttttctga attagaaaat gcttgggata
     6841 aaacactaag atatttgaca cgaatgagtg ttattgttga aaattctatt gatttatcat
     6901 tggaacgaga agttcctgat attctatgtt cagcattgac tgatgattgt attggtcgtg
     6961 gaaaacacct taaagaaggt ggagcagtat atgattatat atcaggattg caagttggaa
     7021 ttgcaaattt gtcggattcg ttagctgcaa ttaaaaaatt ggtgtttgag gaagaacgta
     7081 taagcccaag tcaactttgg catgcactgg aaacagatta tgccggagaa gaaggtaagg
     7141 tcattcaaga aatgttgatt catgatgcac ctaagtatgg taatgatgat gattatgctg
     7201 acaaattggt tactgctgct tatgacattt atgttgatga aattgctaaa tatcctaata
     7261 cacgttatgg aagagggcct attggaggaa ttcgttattc aggaacatct tctatctcag
     7321 ccaacgtagg gcagggacgt ggaacattag caactccaga tggacgcaac gcgggtacac
     7381 cgttagcaga gggttgttca ccatcacata atatggatca acacggccct acatctgttt
     7441 taaaatctgt ttcaaaatta ccaacagatg aaatcgtagg tggggttctc ttaaatcaga
     7501 aagtaaatcc tcaaacgtta gccaaagaag aagataaatt aaaactaatt gctttgttac
     7561 gaacattctt taatcgttta catgggtacc atattcaata caatgttgtt tccagagaga
     7621 cgttgattga cgctcagaaa catcctgaaa aacacagaga cttaattgtt cgtgttgcag
     7681 gatactctgc attcttcaat gttctttcta aggcaaccca agatgacatt ataggacgta
     7741 ctgagcatac tttgtaaaat aaagaggttc tttttatgga atttatgctt gacacattaa
     7801 atttagatga gattaaaaag tggtctgaaa ttttgccgct agctggggta acttcaaatc
     7861 ccactattgc aaaaagagag ggttctatta atttttttga acgaatcaaa gatgtaagag
     7921 aattgattgg ctctacaccc tctattcatg ttcaggtgat ttctcaagat tttgaaggca
     7981 tcttaaagga tgctcatgaa attcgaagac aagcaggaga tgatatattt atcaaagtac
     8041 ctgttactcc agctggatta cgtgcaataa aggtgctaaa aaaagagggc taccatatca
     8101 ctgcaacagc tatttataca gttattcagg gattattagc tatcgaagta ggagcggatt
     8161 acctagctcc atattataat agaatggaaa atctgaacat tgattcaaat tctgtcattc
     8221 gtcaattagc tcttgctatt gatagacaga actctcctag taagatttta gctgcatcct
     8281 ttaaaaatgt agcacaagta aataatgctt tagctgcagg tgcgcatgct gttacagcag
     8341 gagcagatgt ttttgaatca gctttcgcca tgccatctat ccaaaaggcg gttgatgatt
     8401 tttctgacga ttggtttgtt actcaaaata gtcgttccat ttagatagag aggaaataca
     8461 tatgagaatt tttgctagtc cttctagata tattcagggg gaaaatgcct tgtttgaaaa
     8521 tgccaaatca attttggatt tgggaaatta cccgattcta ttatgcgatc agttggttta
     8581 tgatattgtt ggaaaacgat ttgaagatta tctacatagg tatggtttcc atattgttct
     8641 ggcgctattt aatggtgaag cttctgacaa tgaaatcaat cgagttgttg ccttggctga
     8701 gaaagaaaat tgtgatagta ttatcggtct tggtggggga aagacgattg atagcgcaaa
     8761 agctattgca gatttgattg aaaagcctgt tattattgct ccaacaattg catcgaccga
     8821 cgcacctgta tctgctttat ctgttattta tacagatgaa ggtgcatttg atcattatct
     8881 attttattct aaaaatccag atttagtttt ggttgataca aaagttattt cacaagcccc
     8941 taagcgttta ttagcgtctg gtattgcaga tggtttagca acttgggttg aggcgcgtgc
     9001 ggttatgcag gcaaatggaa aaactatgtt gggacaacag caaacattgg ctggagttgc
     9061 aattgcgaag aaatgtgaag aaacgctgtt tgcagatggt ttacaggcta tggcagcttg
     9121 tgaagctaaa gtggtgacac cagcattaga aaatattgtt gaagctaata ctttattgag
     9181 tggcctaggt tttgaaagtg gaggattagc tgcggcgcat gcaattcata atggttttac
     9241 tgcattgaca ggcgacattc atcatttaac acatggtgaa aaagtagctt atggaacttt
     9301 agtacaacta ttattggaaa atagacctaa agaagaactt gataagtata ttgagtttta
     9361 caaaaaaatt ggtatgccaa caactctaaa agaaatgcat ttggatcaag ttggatatga
     9421 tgatttaata aaagttggta aacaagcaac tatggagggt gagacaattc atcagatgcc
     9481 gtttaagatt tcgccttcag atgttgctca agctattatc gctgtagatg cctatgtaaa
     9541 ttcaaaataa acaataagga ctactgtttt ccaaatggta gtcttttatt gatcctcgta
     9601 ttgaat
//
LOCUS       DAVKQL010000076         8725 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVKQL010000076 DAVKQL010000000
VERSION     DAVKQL010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8725
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.076"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(284..1891)
                     /locus_tag="VW561_001876"
     CDS             complement(284..1891)
                     /locus_tag="VW561_001876"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEU9944095.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(2281..2868)
                     /locus_tag="VW561_001877"
     CDS             complement(2281..2868)
                     /locus_tag="VW561_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEU9944096.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(2996..3307)
                     /locus_tag="VW561_001878"
                     /pseudo
     CDS             complement(2996..3307)
                     /locus_tag="VW561_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(3205..3621)
                     /locus_tag="VW561_001879"
     CDS             complement(3205..3621)
                     /locus_tag="VW561_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944097.1"
                     /translation="MKVNIVDLHPTQLYLSEKKLQDIQMLYQSAETNQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLKNHI
                     LPLWIGMVSFFGLKSRRRENGFQLGILHYLRIISPL"
     gene            complement(3649..4299)
                     /locus_tag="VW561_001880"
     CDS             complement(3649..4299)
                     /locus_tag="VW561_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU9944098.1"
                     /translation="MKDKQTFLMKGSFALLLFVILGYMVKFYPETLVGFDQSIQTAIR
                     GDLPDYLTILFRSLTHLIDIPVIITWVVIIAFVFYRKRWKIESFFMLGNLALAGLLIV
                     TFKNIYQRPRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDQVWRKIVQI
                     VLGLYLVSVLVSRVYLGVHYPSDVLASFCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(4289..4852)
                     /locus_tag="VW561_001881"
     CDS             complement(4289..4852)
                     /locus_tag="VW561_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9944099.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(5017..5251)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(5305..5808)
                     /locus_tag="VW561_001882"
     CDS             complement(5305..5808)
                     /locus_tag="VW561_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEU9944100.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(6001..6831)
                     /locus_tag="VW561_001883"
     CDS             complement(6001..6831)
                     /locus_tag="VW561_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9944101.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            complement(6816..8498)
                     /locus_tag="VW561_001884"
     CDS             complement(6816..8498)
                     /locus_tag="VW561_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9944102.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQTAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLADLDNLSIS
                     KGQVQLQTELVKETPALQSLFRLEEVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
BASE COUNT         2620 a         2000 c         1600 g         2505 t
ORIGIN      
        1 taatttgact tttctgatag agatgttcac atcttatttc aattcactat acaatccagt
       61 tcctaaaact ttgctagaaa ttgatttggc tttccgaatt aatttgttca aattttattt
      121 catttcaata tactgataaa tccattcact agcacagggc aagagggagt cgctattgat
      181 ttcaacggta gcaggtacat acagaggacc cctacctcag atatactgca atatcatcgt
      241 atgcaaaaaa cctctgagat ttttctcaaa ggttctgatt ttgctaattg ctgttctcaa
      301 ccgctgcagt aacaaaggca gtgtagagtt cttctggtcg gtttggacgg cttgacagtt
      361 cagggtgata ctgacaagct acaaagaatt tattttcagg aatttccacg atttctacca
      421 aacgattgtc tggagaaact cctgaaaaga caaaacctgc tgcctcaaac tgctcacgga
      481 aggcattatt aaactcataa cggtgacggt gacggcgttg caccacttct tgattgtgat
      541 aagcagcagc agccttagag ccacgtttca acttagacgg ataaagtccc aaacgaaggg
      601 ttccacccat atcctcaata tcaatctgat cacgcatgat atcaatgata gggtattttg
      661 tttctggtgc aagctctgca gaattggcac cttcaagacc taaaacgtga cgagcaaact
      721 cgatacatgt caactgcatt cccaagcaga ctcccaacat tggaacatca ttttcacgcg
      781 catagcggat ggcttggatt ttcccttctg taccacgttg accaaaacca cctggtacga
      841 tgatcccgtc cgcatcagac aagagttctg ctacattctc tgctgtcaca tcattggcat
      901 tgacccaatt gattttaact tctacatcat tgacatagcc agagtgtttc aaggcttcga
      961 ccactgagat ataggcatct tgcaactcca catacttacc aacaagggaa atcttaactt
     1021 gtttcttgag gttcatgacc ttgtccacca tggctgacca ttctgtcata tccgctgctg
     1081 gtgcgtctaa tttcaaatga tcacaaacaa tttggtccat cccttgtgcc tgcaagttca
     1141 gtggaatttg gtaaaggtgt tcaacatcca acgattcgat aacggcttct ggtgccacat
     1201 cacagaactg ggccagttta tttttaattc cttgaccagc tggctcttct gtacgaataa
     1261 ccaacatatt tggttggatt cccaagccac gcaattcttt gacagagtgt tgggttggtt
     1321 tggttttcat ttcaccagca gccttgaggt aaggaagcaa ggttgtatgg atatacatga
     1381 cattatccgc acccacatct gccttcatct gacgaagagc ctctaggaat ggcaaggact
     1441 cgatatctcc tactgttcca ccaacctctg tgataatgac atcagagtcg gtcgttagag
     1501 cggcacgctt gattttttct ttcaaagcat ctgtgatatg aggaatgact tgaacagttg
     1561 ccccaaggta ttctccacgg cgttctttac gaagaacttc actgtaaatt ttcccagttg
     1621 tcacgttgga atatttgttg agattgatat cgataaaacg ttcatagtga cccaagtcca
     1681 aatctgtctc agctccgtca tctgtcacaa aaacttcccc gtgctggtaa ggactcatgg
     1741 ttcccggatc aatattgata taagggtcaa acttttgaat ggttactttg agaccacgat
     1801 ttttcaagag acggcctaga ctcgctgcca caatcccttt cccaatagac gataccacac
     1861 caccagttac aaaaatatat ttcgtagaca tagattcctc tttctaaaat gctcaaggtc
     1921 ttgttaacta cgaggggata tagaaaacaa gaccctacta ataagaataa tctgggacga
     1981 ctgcaccaga ttcacaacta atactcttcg aaaatctctt caaaccgcgt caacgtcgcc
     2041 ttgccgtaga tatgtgttcc tgactttgtc agtcttatct acaacctcaa aacagtgttt
     2101 tgagcagcct acggctagtt tcctagcttg ctctttgatt ttcattgagt ataaaataca
     2161 agaagataac ttcttgaaaa aacaaaaata gctccctaag aactagggag ccccgacctc
     2221 taaaagaggt gcccaaacaa tatgatacct aaaaatagga taattgtcaa tagctaactc
     2281 ttattcctcg ctggtttcag catcgtcatc tgaaaactcg tcgtcgtctt cgttgagatc
     2341 cacatcttct cccaagtcat ctggagcgat ttcgttgatt tctgcatcat aagcttccac
     2401 ttcatttttt tcatcatctg gattttcatc atcgtatgaa agagctggat ctgcttcgta
     2461 tgcatcttcg tcttctggat catctgcatt gtagtcaatg gcatctgaat caccatccat
     2521 aaaggcattg acacgttttt tcttagcttt tggtgctact tcatcgtcgt cattttcttc
     2581 aagagcgatg atttcttcgt cgatttcgtc cacaccatac catgaacgaa gaccccattt
     2641 gttgtcccca agtgagatga agctaccgtc aaagttcaac tctgtgtaga acaaaggcaa
     2701 agcttcgcgg atatcgctgt ttgatgttcc aaggtagttt tgaatttcgt ttacaagatc
     2761 gctaaaatgc atctcatgat cgcgaccacg aagttccaag atagcacgcg ctacctcaat
     2821 catagatagt tcactttttt cttgcccagc aaatacttct aattccaaag cgtttctcct
     2881 catttatact actatcgcca gagcgaacag actctgacct cattttatca tttactcttt
     2941 attttacgat aattttgcgg aatagtcaaa ggttaagggg gagaaagtgg caggattaga
     3001 ctaattccaa tataaaactc cttccttttt ctgttgctcc attttccaca aatccaagcg
     3061 acttgaaaca cctcctagaa gcatgattgt aggtgtagat ttccttgact ctcaattctt
     3121 tccatccttt tactcgagcc aattcaatca aagcacttag aatctttttt ccaagtcctc
     3181 gatgttggta agcggaattc ccaatcacaa tggggagatt atcctgagat agtgtaatat
     3241 ccccaattgg aaaccattct cccttctcct tgacttcaat ccaaaaaagc tcaccatgcc
     3301 gatccaaaga ggtaagatat gattttttaa atcaaaaaca gagtaaatct ttctttcctc
     3361 gcaagcttgc gcatagagat gatatagttc atcaccacca tctctatccc actcagcaga
     3421 aatcgtatcc cgacctgcca ataaagcctg ataagccctg tgatgcccat ctgtaatcag
     3481 caaacaatct ccaaaggcaa gaatactgat tggatcgact tggtttgttt ctgccgactg
     3541 gtaaagcatc tgaatatctt gcaacttctt ttctgataaa tatagttgag tcggatgaag
     3601 atctactata ttgactttca tttctttctc ctcaagggaa ttcgatactc acttctgttt
     3661 gcctttaaat cgccattgga agcggagctt gtcatagaag ggaaactcga taaacaagac
     3721 tcccaaaccc acacagaaac tggcaagaac atctgatggg tagtgaactc ccagatagac
     3781 tcttgatacc agcacactga ctaggtagag gccaaggacg atttgtacga tttttctcca
     3841 gacctgatct ttaatccgct gactaagaat aacaatcaaa gtccctacca tcagcgttac
     3901 agctagagaa tgcccacttg ggaaggaaaa tcccttctcc tccaccagat gtaagatagc
     3961 tggtcgtggg cgctggtaga tatttttaaa ggtcacgatt aaaagacctg ccaaagccag
     4021 atttcccagc atgaagaaac tttctatctt ccatcgctta cgataaaaga caaaagctat
     4081 aatgacaacc caagtgataa tcactggaat atcaatcaga tgtgtgaggg atcgaaaaag
     4141 aatagtcaaa taatctggta agtctcctcg aatggcagtc tgaatcgatt ggtcaaaacc
     4201 gaccagcgtt tcagggtaaa atttgaccat atagccaaga ataacgaaaa gtaaaagggc
     4261 aaaactgccc ttcattaaaa atgtttgttt atctttcata gtgttttaag gttggtttca
     4321 aaaggacgta caacaaccag aatgaaacgg aaaagattac accctcaatc aagttaaaag
     4381 gtaataccat ggtcattagg tagttggaaa gtcccaaaat ttttccaata tcaaagttag
     4441 caaacttagc gtacaaagga acagcataaa catagttgag aaccaacatg gccaaggtta
     4501 aaccaacagt tccagctaga gagcctagta ggaaacgaag ggttgtccgt tcctttttcc
     4561 aaatcaaagc aaatacgatg acaaaaactc ccaaagctac gatattcatc ggcaaaccaa
     4621 tgtaagtatt cactccttgg ctgttaagaa gcaatttcaa gagtgagcga agcaagagca
     4681 ctcctagagc agcaggcaaa tccatgacca ccagacccac aaggactggc aagatactaa
     4741 attcgatctt gaggaaagat gccgctggta aaagcggaaa gtcaaagtac atcagcacaa
     4801 atgagatggc tgatagaatt gcaatggtcg aaagtcgacg tgtgtttgtc ataacaggtt
     4861 cctccaattt tctataaaat cagaagaagt tggaaaggat tcctctatct attctcactt
     4921 tttatatccc aaaagttccc tcttactcta ttaaagaaaa acaaagcaag tggttacaat
     4981 ccggctataa atctatcaaa acagacaaag ctattctttc gtcttctccc atccagacta
     5041 tactgtcggt tgtggaatct caccacatca gcttgcgctc gcggacttct ttaaagagaa
     5101 ggaaatagag atttttcttt tagactaggc gacaagctgt aggcataact tctggttagg
     5161 caaagcttgt caacaaagta taaaaggaaa agatcatttc ctagttaaag tcaccgccgg
     5221 tcgggaatca caccctgccc tgaagactct tttatcataa caaaaaacgc ttgcaagcgc
     5281 aagcattttg ttcattttca ttttttattt caatttatcc acttcatagg tatggacgag
     5341 ctcaagacct gcaaagttct gctggcgaag agcctcatag acaatcatgc agacggtatt
     5401 agacacattg agactgcgga catgttcatc attcatggga atacggagag ccttctcagg
     5461 atgttctcgc ataaaatctt caggtaagcc cttgtcttca cgtccaaaga gaaaataatg
     5521 gtcctcgtca gtcgataaat ccacctcaga atagactttt tccgcgaatt tcgaaatcag
     5581 atagagtttt cccttcatct gagatatgaa atcttccaaa ctctcgtaaa aataaatctc
     5641 tagcttatcc cagtaatcca atccagcccg cttcatcttg cggtcatcaa taggaaagcc
     5701 cataggcttg atgatgtgga ggggagaatt ggtcgcagcg caagtacgcg cgatattgcc
     5761 cgtattttgt ggaatttgag gttcaaataa tacaatgtga tttgtcatga cttgcttcct
     5821 ttcaccattg caaaaaaata gccacactgc ccggagtcaa gctcagcaaa cagcgtggtt
     5881 aaggcatcgt taacttacct cacaacaggt ttgaagtaaa tcagcgaaac tactttctta
     5941 gtataacact ttcagaatca ttgtcaatag aaatgacttg attttttcaa ttttttcaag
     6001 ctatttccaa gggttgtaaa atcgtccctg attctgcaag ataagtagta aactaactac
     6061 taaaaacaag gttgccaaga gcaaggtaat atagtctcct tttttcaagg cctgataact
     6121 ataccatgtg cgttttttct ctttcccaaa gcggcgaagc tccatggcag tcgcaatggt
     6181 atcaatgcgt tctagcgagc taaaaatcaa gggcgtaata atgagcagat tgcctttgat
     6241 tcgttgcata agagaagctt tcttggataa ttccatccca cgcgcctcct gagacatctt
     6301 gatagtaaag aattcttcct gcaaatctgg aatatagcgc aaggtcaggc tgacagaata
     6361 agcaatctta tagggcacac caatttgatt taaactggaa gcaaactgac taggatgggt
     6421 tgtcatcaaa aagataatag ccagaggaat ggtgcaaaga tacttaatgg ccaaatttag
     6481 cagataaaag agctcctggc tggttagagt gtagacaccg attccctgcc aaatcacact
     6541 tctctctcca taaagtccaa ccccatactc gggagaaaag agatagacca tcaaaacgtt
     6601 taaaacggca aataccgtcg caaaaacggc tacaaaggaa acatctttaa agcgaatttc
     6661 tgataaatag aggagaaaga ctgaaaagat ggcaatcagc aagagcattc tggtatcata
     6721 gctaatcatg gccgccaatg ataccagaat gaaaaagaga agtttcccag ctcctgacaa
     6781 gcgatgaatc acagtatctc tatgctggta accgattaat ttagcttgca tccctctctc
     6841 ctttctttgt aaaatgccgt taaatccagt ggatccacat ctagtttctt agccaagtta
     6901 aagatggagg tttcttttag attggctttt actaacagct caggatcgct caacagactg
     6961 gctggaacag tatcggcaat caattctcca tccaccatga caaggacccg gtctgaataa
     7021 tccagcatca attgcatatc atgggtaatc atgacaatgg tatgcccttt ttgatgtaac
     7081 tcttcgagaa attccataat ctcagtatag ttcttctgat cttgacctgc agtcggttca
     7141 tctaggagaa taatttcagc tcctaagacc aaaattgaag caatggtgac acgttttttc
     7201 tgaccaaatg acagggcaga aataggccaa ttacggaatt cataaagtcc acagattttc
     7261 aaggtttcat atactctcgt ttcaatttcc ttctcatcca cacctcgcaa acggagccct
     7321 agagccacct catcaaaaat catattggtt gaaatcattt gattaggatt ttgtagcaca
     7381 tatcctactc gttccgcccg ctctgcaaca gaatcgcctt ttatatcctg tttttcccaa
     7441 agatagcgtc cttccgtctg aataaagcta cttatagcct tggctagagt tgatttccct
     7501 gctccatttt ttccgacaat agcaatcttt tcaccctttt taatatctaa atgtagggat
     7561 tttaaaatcg gtctatcatc ataagaaaaa gatacttcct ctagtctaaa gagtgactgc
     7621 aatgctgggg tttcttttac cagttccgtc tgtaactgga cttgcccttt tgagatagac
     7681 aagttatcca gatctgctaa ttgttcttcc ttgactaagt ccacacctaa ttgacggaga
     7741 gtcgttagat aaaggggttc tcgaattcca ttttgagtca ataaatcagt cgcaagcaac
     7801 tggtcagggc tcccattaaa aaggatacga ccatcgttta tcaagacaat ccgatccaca
     7861 gggcgatgca gaacgtcctc caaacggtgc tcgataatca gagtcgtcgt cccctcttcc
     7921 ttatgaatct ggtcaatcaa ttcgataata tcctgacctg acttgggatc tagattggcg
     7981 agtggctcat caaacaagag aatcggactt tcatcaatca agacaccagc cagactgact
     8041 cgctgctttt gtccacctga caaatcctga ggacgctgat ccagtaaagg aagaaggtcc
     8101 agcttttcag cccatttata aacacgacct ttcatctcat ctagggctgt cacatcattt
     8161 tccagagcaa acgccaaatc ttctgccaca gacaagccaa taaactgccc atctgtatcc
     8221 tgcaaaactg tgctaaccag atgagactta tcatagatgc tcatatcaaa ggctgtttga
     8281 ccctttatca aaaattctcc atatgtctga cccttgtaaa tattgggaat aatcccattc
     8341 aaacactgac ccaaggtaga tttacctgac ccagatggtc caacaattaa gactttctct
     8401 cccttgtaaa tggtcaagtc tatcccttgc aaggtcggtt cttgttgtgt ttcataccgg
     8461 aaagagaaat ccttccactc aattatagct tctttcatct tactctcttc attcgcttct
     8521 tagacttcta ttttatcata aatcaagccc ttcttgcagt ctcttctctt aaaatcttag
     8581 cgccaaaaag attcctatcc tagcttactt gcctaactaa tctataaaca tcgaaaaaga
     8641 ctagttgccc agccttcccc atcattttat actcttcgaa aatctcttca aaccacgtca
     8701 gcttcgcctt gccgtaggta tggtt
//
LOCUS       DAVKQL010000077         8712 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVKQL010000077 DAVKQL010000000
VERSION     DAVKQL010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8712
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.077"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            <1..399
                     /locus_tag="VW561_001885"
     CDS             <1..399
                     /locus_tag="VW561_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU9944103.1"
                     /translation="SEIIAYNLSTSPNLEQVKSMLEQAFTEKHYENTILHSDQGWQYR
                     HDSYHRFLESKGIQASMSRKGNSPDNGMMESFFGILKSEMFYGFEKSFQSLKQLEQAI
                     IDYIDYYNNKRIKVKLKGLIPVQYRTKSFG"
     gene            complement(527..730)
                     /locus_tag="VW561_001886"
     CDS             complement(527..730)
                     /locus_tag="VW561_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9944104.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(723..1805)
                     /locus_tag="VW561_001887"
     CDS             complement(723..1805)
                     /locus_tag="VW561_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU9944105.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTDFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(1931..2713)
                     /locus_tag="VW561_001888"
     CDS             complement(1931..2713)
                     /locus_tag="VW561_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEU9944106.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(2713..4056)
                     /locus_tag="VW561_001889"
     CDS             complement(2713..4056)
                     /locus_tag="VW561_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEU9944107.1"
                     /translation="MNLKTTLGLLAGRSSHFILSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILEAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECILVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(4155..5471)
                     /locus_tag="VW561_001890"
     CDS             complement(4155..5471)
                     /locus_tag="VW561_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEU9944108.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQSLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            5664..6890
                     /locus_tag="VW561_001891"
     CDS             5664..6890
                     /locus_tag="VW561_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEU9944109.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGA
                     GIVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            7089..8663
                     /locus_tag="VW561_001892"
     CDS             7089..8663
                     /locus_tag="VW561_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9944110.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQNIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
BASE COUNT         2483 a         1701 c         1941 g         2587 t
ORIGIN      
        1 agcgaaatta tcgcctataa tctttcaact tcgcccaact tagaacaagt gaagagtatg
       61 ttagaacagg cctttacaga gaagcactac gagaatacga ttctccatag tgaccaaggc
      121 tggcaatacc gacacgattc ttatcatcgg ttcctagaga gtaagggaat tcaagcatcc
      181 atgtcacgta agggtaacag cccagacaat ggtatgatgg agtccttctt tgggattctg
      241 aaatcggaaa tgttttacgg ttttgagaag tcgtttcagt cgcttaagca attggaacaa
      301 gctattatag actatattga ttactacaac aataaacgaa ttaaggtaaa actaaaagga
      361 cttatccctg tgcaatacag aactaaatcc ttcggataaa ttaattgtct aactttttgg
      421 ggggcagttc attctacacc cactattatg cgttttattg tgggaagatt ttcctcattt
      481 tctagtgaaa ttgaactttt tctaacatct tttgatagat gacttcttaa tcaattatca
      541 tttccattgt tgtttcttgg ggtttcatcc cttgtctttg ataaaagcgg agagcaccct
      601 cgttatcatt ccagacactt agggttagat tattgcagcc aatttcttta gcataatagc
      661 agaaaaatga caataaaata gcccctagat attttgaaag gaaagcatag gggctatata
      721 gtttaatcaa aataaagcaa atctttgctg gtgcttgtaa agaggtcgaa gccatccttg
      781 gtaacaacac cgcagtcttc aatacgaaca ccgactttac cagggatata gatacctggt
      841 tcaacagaga agcacatgcc ttcttcgatg accatgtcgt ttccttccat gatagatggg
      901 aattcatgga catccatacc gataccatgc ccgagacggt ggttgaagta ctcaccataa
      961 ccagcttttt cgatgacctc acgggcagcg cggtccactt catgagcagt cacacctggc
     1021 ttgataaagt caagagcagc ttgttgggct tcaagagtca agttgtaaat atctttcttg
     1081 aattggtctg gtttgccgac agcgactgta cgagtcatat ctgacgcata gccattgacc
     1141 agaacaccca ggtcaaagag gagaagagca tcattttcaa ccttattagc tgctggaatg
     1201 ccgtgtggat tcgcagcatt atcaccagtc aagaccatgg tatcaaagct catttcgtaa
     1261 ccttcacgtt tcatggcaaa gtcaatttgt gcgatgatat ctgtctcagt cttatcaaga
     1321 gaaatattgt caaaaccaac atgaacagcc ttgtcagcat aaagacctgc aaccatcatt
     1381 ttttgcactt catcagctga tttgatgagg cgcatgcgtt ggatacgagg agtgaggttg
     1441 tcaaactcag cagtctcaaa aactgttttc aaaccatggt atttggtcaa gatgagattg
     1501 tcaaactcaa cagcgacacg tttgaagtca agttgtggaa gagcatgttt gattttttgc
     1561 catggatttt cagaatcgac atagcccact actgggaagg aaacggtgct acttgcacgt
     1621 tctacttcaa gagctgggac aaagaggaga ggttcctgat ctgctaggac aaagaggaac
     1681 atttggcgtt catggggatc actgtaaaaa tcagtgaggt aattgattgt gacggggtca
     1741 gatacgacag cgacgtctag tttttctgat tcaagatatg ttaggatttg ttgtaattta
     1801 gacatatgct accttctttc tacccctcta ttttggcaaa aaatgagaga aaatgcaagg
     1861 gagaagggta aaagaaaaga caaaaaactc cgacaagata acggagttct ttgatatcat
     1921 tttcctttgt ttaagaaaag tcagccttgc ttttgacgtc actgtactct tcagcgattt
     1981 cttggctgag aatgtcctca taggcaggga gttggatgtc ctgaccatat ttcttcttga
     2041 gggcagtagt aactaggcga taagccagat tggcattacg agagaggata ggcccgtgga
     2101 agtaggaacc aaagacattc ttataatgaa ccccttcacc gaccttttct tcgttgtttc
     2161 catttccata gacaacctgt cccagcggtt tttggtcatc agagaggaag gtacgacctt
     2221 ggtgattttc aaatccatag taggtttcat cgaaatcttc attgtgaatc ttgatgtcac
     2281 cgataaaacg gttattggtc tggttgagcg tgtagtgtcc catgacccct agcccttcga
     2341 tacgttttcc tgaagcttca acataatatt gacccaatag ttggaaacca ccgcagatag
     2401 ccagaactac accgtcgttt tggatgtagt tgtcaatgct ctctttttta gcaggtaggt
     2461 cgtctgcaat gatactttgt tcaaagtctt gaccaccacc gaaaaaggcg atgtcgtagt
     2521 gattttcatc aaagtcatca tggagagaaa cgatgtcaac ggtcacatgg gctcccagtt
     2581 tttcagccac atacttgagc atgaggatgt ttccattgtc cccgtaggta ttcatgagat
     2641 ttccgtagag gtgggcaatg ttgagctgat aggggtaatt gccatctttt gaggaaagtg
     2701 aagtataaac cattagttca tctcctttct aacaatctga cgactagcca gcagttcacg
     2761 aaattccagc atggcagtat aagttgccag aatataggca tgcttgcagt cttgattctc
     2821 aatggtcttg agaacttgct ccagattact cgtttcagtg attttctcag ctggatagcc
     2881 agtcactcgg aggcgacgag cgatttcaga atgacgaaca ccgccagcgt tgatttcagg
     2941 aatgtccatg tcagtgattt gctcaaagtc tgcatcccag atccagctag tgtcaattcc
     3001 atctgcatag ttagcattaa ggaggacaga taggctaaat ggataaggtg ctagtttgat
     3061 catttcgata gcttgggttg caccgactgg atttttaatc aagacaagga tacattcctt
     3121 gtcaccgata tgaaaggttt cttggcgtcc aaagacagca cggctcttgt caaatccctg
     3181 tttgatgagt tgggaatctg cgccgaggaa acgggcgatg gccacagcag ctagggcgtt
     3241 atagatatta tagagcccgc cgatttggat accgtattct tggccgtcta tgacaaagcg
     3301 agagcgattg ttggtcaact caaccagttt tgtcaaacga tagtcgagat caggacgttt
     3361 acatccacaa ccttcacaga tataggcacc caagtttgca taggtattat gctcatattt
     3421 gaggatgcct tggcagtcag gacagagaat cccttcggta ttgtagtgag ccagttgggc
     3481 tggtcctttt tccaagtcaa aaccaaaata ctctataggg tttggaatag ttggcttgta
     3541 gaaaagtgga ctgtctccgt taaggagaac agtagcagtt ggaactttcc gaatggcttc
     3601 caatatcatg ttataggtag tatagatttc accgaaacgg tccatctggt cacggaagat
     3661 attagtaatg acaaaaagac taggctggat atagtcacag atacgagata gactggcttc
     3721 gtcaatttcg aggacggcaa tatttttccc agtttttgaa gatttggctg ttaggaaggt
     3781 tgttgcaatc cctgtaatca tgttggcacc gcttgggttg gttagaactt gaccataaac
     3841 ctcttttaaa atgccgacag tgagggcagt tgtcagggtt tttccatttg ttccagtgac
     3901 aacgacaatc tcgtagttct tagctaggtt ttgtaaaata tctttatcaa attgaagggc
     3961 gactttacct gggagcgtac ttccacggcc aagacggctt aaaatgaagt gggaagaacg
     4021 cccagcaaga aggcccaaag tagtttttaa gttcatgttt ctattatatc ataaaagagg
     4081 agaaagacag atttgagatt ccgcagaagt aaaaacagcc caaagagggc tgttcgatac
     4141 gattaaatct caacttaaat cgcaaacaag tcattcaagt tctcagccaa ggttgggtga
     4201 gtgaagattt gttttgtgaa gtaagtgtaa ggaatcttgt tgtccatagc aacagtgatg
     4261 atgttgatga tttcttgaga accttctgag aagatgcttg ctccaagaat ttcttttgtt
     4321 tcagtattga caacagcttt gaaggcaccg cgaaggtctc catttacgtg accacgaggc
     4381 attgctgcaa cggggatttc cttaacagcg tatggaagtt tcaaatcagc tgcttggctc
     4441 tcagtcaaac caacttgtga aagtgcaggt gtgatgaaca tagtatttgg cacattgaga
     4501 cggtcttcaa gtgtgtagct gccatctcca gcaaggtagc tgtaaacaac acggaagtca
     4561 tcaagtgaaa tgtaagtaaa ttgaaggcca ccgttgacat ctccaactgc aaagacacca
     4621 ggaacgtttg tttgacagtg tttgtctact ttaatagcac cacgttcagt tagttcaata
     4681 tctgtatttt caagttgaag tggttctaca tttggtttgc gtccagttgc gtagagaagg
     4741 gcgtcgaaac ggtaagtttc gtcttcagtt acgacaagca cttggtcacc atcgttttta
     4801 atttcagtag tatggatatt ttgaagcaat tcaatgccat cttcttccat gtattgttta
     4861 gcaagagctg cgatggaagg ttctgcacga ggtaggaatg tatccaaggc atctaggact
     4921 gtgaccttgc ttccaagttt gttgtaaagg ccggcaaatt caagaccgat atttccgcca
     4981 ccaaggattc caagtttttc aggtaatttg tccaagcttt ggatacctgt tgagtcaaag
     5041 atgtttttgc ttgtagcaag tccagggatt ggcaagacgt ttgaaacagc accagtgttg
     5101 atgacgattg tttcagcagt cagttctttc ttttcatcac cagcttggat ttcgatgact
     5161 ttatttgaaa ggaagtgagc ttccgcatca aagatatcta cgcctgtacc agcaacagtc
     5221 gcatagtttt taccgttgag gcgaccagtg atcgtgtttt tagtagcaat gacttcttca
     5281 aaagacaagt ccttttcagc agcaactagc aaggttttag ttgggataca accaatgttg
     5341 atacaagttc caccgtacat agccttgctg cgttcaacga gggcaacttt tttgccagct
     5401 gaagccaatt tacctgctag tgttttacca gctttaccaa atccaataac aattaaatcg
     5461 tatgttaaca tttatcttcc tcctattcga atttcattct agcacaagaa aaaaactttt
     5521 gcacaattct gctacggaaa agacagaaaa agataaaaac gctttccaga caaaaaacta
     5581 gtaatttttt aacaagcttg ataaactaaa atcctatcta tttttatata agatagaata
     5641 aatgttcgaa aatgggaggt tttatgaaat cgaatcgtta tattattgcc tttgctgggg
     5701 tcattttaca cttaatgctg ggttcgactt atgcctggag tgtttatcgt aaccctatta
     5761 ttgaaaaaac gggatgggat caggcttctg ttgccttcgc ctttagtcta gcaatctttt
     5821 gtttgggctt atcggctgca tttatggggc gtttggtaga aaaatttggt ccgaaagtca
     5881 tgggaagtct atctgctttt ctatacgcag gtggaaatat cttaacagga tttgcaatag
     5941 accgtcagga gctgtggttg ttgtatctcg cttatggcat tttaggtggg cttggtttgg
     6001 gagcaggcta tattacccct gtgtcgacga ttataaaatg gtttcctgat aaacgtggtc
     6061 tcgcaacagg tttagcgatt atggggtttg gttttgcttc tttattgact agtcccatag
     6121 cgcaacacct catcgcaggg gcagggattg tagaaacttt ttatatttta ggagcaagtt
     6181 actttattat catgctccta gcttcacaat tcattaagcg tccaaatgag caagagcttg
     6241 caattttatc ttcttcaggg aaagaaaaaa cagcctcttt gacgcaagga atggctgcaa
     6301 atcaggctct aaaaagcaat cggttttata tgctttggat tattttcttt atcaacatag
     6361 cttgtggttt aggcttaatt tcagcggcat cgccaatggc acaggagatg gctggcttgt
     6421 ctacaagtca tgcagcagta atggtgggtg ttttggggat tttcaatgga tttggtcgct
     6481 tgctctgggc gagtttgtct gactatatcg gtcgccctct aacctttagt atattactgc
     6541 ttgtcaatct tttcttttct ctctcacttt ggctctttac agattccgtt ttatttgtag
     6601 ttgctatgtc tattttgatg acttgctatg gagctggttt ttctttgatt ccagcttatc
     6661 tcagtgatat ttttggaacc aaggaattgg ccgctctgca tggttatatt ttaacagctt
     6721 gggcaatggc tggtttagcg ggacctattt tattagcaga gacttataaa atggctcatt
     6781 cgtacacaca aaccttgttc gtttttctca ttttatacag tatcgccttg gctttgtctt
     6841 attatctagg tcgttcaatc aaaaaagaaa gtcaaaaacc gcttacatga ttttctgtag
     6901 aaatggggct atcttttgct ctggtcttgt gttatactag ataggttgca aagaaaacag
     6961 tacttttctt ttgtggaaaa gaagcaacac gattttatat ctagttatat gaaaatacgt
     7021 cataaaaaga aaagtataaa ctaagcccta tagggtggct tcgcaccacc tttagaaaga
     7081 agaataacgt gaaatttaat gaattaaact tgtctgctga tttgctggca gaaattgaaa
     7141 aagctggttt tgtagaagct agtcctatcc aagaacaaac tattcctttg gcccttgaag
     7201 gcaaggatgt tatcggtcaa gctcagactg gtacaggaaa aactgcagcc tttggcttgc
     7261 ctacccttga aaaaatccgt acagaagaag cgactatcca agccttggtc atcgctccaa
     7321 ctcgtgaact agctgtccaa agtcaagaag aactcttccg ctttggtcgt agtaagggag
     7381 tcaaagtccg ttcagtatat ggcggatcaa gcattgaaaa acaaattaag gctcttaaat
     7441 ctggtgccca tatcgtggtg ggaactccag gtcgcctctt ggacttgatt aaacgcaagg
     7501 ccttgaaatt acaaaacatt gaaaccctta tccttgacga agcggatgaa atgcttaaca
     7561 tgggcttcct tgaagacatc gaagccatta tttcccgtgt acctgagaac cgtcaaactt
     7621 tgcttttctc agcaactatg ccagatgcca tcaaacgtat cggtgttcag tttatgaaag
     7681 cccctgaaca tgtcaagatt gcggctaagg aattgacaac agaattggtt gaccagtact
     7741 atatccgtgt taaggaacaa gaaaaatttg acaccatgac tcgtctcatg gatgtggcac
     7801 agccagaact cgctattgta tttggtcgta ccaaacgccg tgtggatgaa ttgactcgtg
     7861 gtttgaaaat tcgtggcttc cgtgcagaag gaattcatgg cgacctagac caaaacaaac
     7921 gtcttcgtgt ccttcgtgac tttaaaaatg gcaatcttga tgttttggtt gcgacagacg
     7981 ttgcagcgcg tggtttggat atttcaggtg tgacccatgt ctacaactac gatattccac
     8041 aagatcctga gagttatgtt caccgtatcg gtcgtacagg tcgtgctggt aagtcaggtc
     8101 aatctattac ttttgttgct ccaaacgaaa tgggttacct tcaaatcatt gaaaacttga
     8161 ctaagaaacg catgaaaggt ctcaaacctg caagtgtaga agaatccttc caatcaaaaa
     8221 aacaggtagc tctcaagaaa atcgaacgtg attttgcaga tgaaaccatt cgtgccaact
     8281 ttgagaaatt tggtaaggat gctcgcaaat tggctgctga gtttactcca gaagaattgg
     8341 caatgtatat cttgagtctg acagtccaag acccagatag ccttccagaa gtggagattg
     8401 cacgtgaaaa accactaccg tttaaaccat caggtaatgg tttcggtggt aaagctaagg
     8461 gaggtcgtgg aggccgtcgt ggggacgacc gtcgagagcg tgatcgccgt ggcaatggtc
     8521 gccgtgatga gttcaaaaaa ggaagtcgtg gcaacgatcg ttttgataag gaaaaacgtt
     8581 accgtaagga taataaaaaa ccacgcaata ctttaagtga aaagcaaaca ggctttgtta
     8641 ttcgtaacaa aggcgataaa taagaaaaag cggttcaaaa tgaatcgctt ttttatataa
     8701 ggagaccgaa tg
//
LOCUS       DAVKQL010000078         8272 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVKQL010000078 DAVKQL010000000
VERSION     DAVKQL010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8272
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.078"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            16..462
                     /gene="argR"
                     /locus_tag="VW561_001893"
     CDS             16..462
                     /gene="argR"
                     /locus_tag="VW561_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9944111.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            513..3047
                     /gene="mutS"
                     /locus_tag="VW561_001894"
     CDS             513..3047
                     /gene="mutS"
                     /locus_tag="VW561_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU9944112.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKAMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            3194..4888
                     /gene="patA"
                     /locus_tag="VW561_001895"
     CDS             3194..4888
                     /gene="patA"
                     /locus_tag="VW561_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEU9944113.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLDALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQDGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(5218..5487)
                     /locus_tag="VW561_001896"
                     /pseudo
     CDS             complement(5218..5487)
                     /locus_tag="VW561_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            5679..7445
                     /gene="patB"
                     /locus_tag="VW561_001897"
     CDS             5679..7445
                     /gene="patB"
                     /locus_tag="VW561_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEU9944114.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTHVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            7584..>8272
                     /locus_tag="VW561_001898"
     CDS             7584..>8272
                     /locus_tag="VW561_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU9944115.1"
                     /translation="MARTVVGVTANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
BASE COUNT         2323 a         1601 c         1947 g         2401 t
ORIGIN      
        1 acgcaagagg attttatgag aaaaagagat cgtcatcagt taataaaaaa aatgattact
       61 gaggagaaat taagtacaca aaaagaaatt caagatcggt tggaggcgca caatgtttgt
      121 gtgacgcaga caaccttgtc tcgtgatttg cgcgaaatcg gcttgaccaa ggtcaagaaa
      181 aatgatatgg tgtattatgt actagtaaat gagacagaaa agattgattt ggtggaattt
      241 ttgtctcatc atttagaagg tgttgcaaga gcagagttta ccttggtgct tcataccaaa
      301 ttgggagaag cctctgtttt ggcaaatatt gtagatgtaa acaaggatga atggatttta
      361 ggaacagttg ctggtgccaa taccttattg gttatttgtc gagatcagca cgttgccaaa
      421 ctcatggaag atcgtttgct agatttgatg aaagataagt aaggtcttgg gagttgctct
      481 caagacttat ttttgaaaag gagagacaga aaatggcgat agaaaagcta tcacccggca
      541 tgcaacagta tgtggatatt aaaaagcaat atccagatgc ttttttgctc tttcggatgg
      601 gtgattttta tgaattattt tatgaggatg cggtcaatgc tgcgcagatt ctggaaattt
      661 ccttaacgag tcgcaacaag aatgccgaca atccgatccc tatggcgggt gttccctatc
      721 attctgccca acagtatatc gatgtcttga ttgagcaggg ttataaggtg gctatcgcag
      781 agcagatgga agatcctaaa caagcagttg gggttgttaa acgagaggtt gttcaggtca
      841 ttacgccagg gacagtggtc gatagcagta agccggacag tcagaataat tttttggttt
      901 ccatagaccg cgaaggcaat caatttggcc tagcttatat ggatttggtg acgggtgact
      961 tttatgtgac aggtcttttg gatttcacgc tggtttgtgg ggaaatccgt aacctcaagg
     1021 ctcgagaagt ggtgttgggt tatgacttgt ctgaggaaga agaacaaatc ctcagccgcc
     1081 agatgaatct ggtactctct tatgaaaaag aaagctttga agaccttcat ttattggatt
     1141 tgcgattggc aacggtggag caaacggcat ctagtaagct gctccagtat gttcatcgga
     1201 ctcagatgag ggaattgaac cacctcaaac ctgttatccg ctacgaaatt aaggatttct
     1261 tgcagatgga ttatgcgacc aaggctagtc tggatttggt tgagaatgct cgctcaggta
     1321 agaaacaagg cagtcttttc tggcttttgg atgaaaccaa aacggctatg gggatgcgtc
     1381 tcttgcgttc ttggattcat cgccccttga ttgataagga acgaatcgtc caacgtcaag
     1441 aagtagtgca ggtctttctc gaccatttct ttgagcgtag tgacttgaca gacagtctca
     1501 agggtgttta tgacattgag cgcttggcta gtcgtgtttc ttttggcaaa accaatccaa
     1561 aggatctctt gcagttggcg actaccttgt ctagtgtgcc acggattcgt gcgattttag
     1621 aagggatgga gcaacctact ctagcctatc tcatcgcaca actggatgca atccctgagt
     1681 tggagagttt gattagcgca gcgattgctc ctgaagctcc tcatgtgatt acagatgggg
     1741 gaattatccg aactggattt gatgagactt tagacaagta tcgttgcgtt ctcagagaag
     1801 ggactagctg gattgctgag attgaggcta aggagcgaga aaactctggt atcagcacgc
     1861 tcaagattga ctacaataaa aaggatggct actattttca tgtgaccaat tcgcaactag
     1921 gaaatgtgcc agcccacttt ttccgcaagg cgacgctgaa aaactcagaa cgctttggaa
     1981 ccgaagaatt agcccgtatc gagggagata tgcttgaggc gcgtgagaag tcagccaacc
     2041 tcgaatacga aatatttatg cgcattcgtg aagaggtcgg caagtacatc cagcgtttac
     2101 aagctctagc ccaaggaatt gcgacggttg atgtcttaca gagtctggcg gttgtggctg
     2161 aaacccagca tttgattcga cctgagtttg gtgacgattc acaaattgat atccggaaag
     2221 ggcgccatgc tgtcgttgaa aaggctatgg gggctcagac ctatattcca aatacgattc
     2281 agatggcaga agataccagt attcaactgg ttacagggcc aaacatgagt gggaagtcta
     2341 cctatatgcg tcagttagcc atgacggcgg ttatggccca gctgggttcc tatgttcctg
     2401 ctgaaagcgc ccatttaccg atttttgatg cgatttttac ccgtatcgga gcagcagatg
     2461 acttggtttc gggtcagtca acctttatgg tggagatgat ggaggccaat aatgccattt
     2521 cgcatgcgac caagaactct ctcattctct ttgatgaatt gggacgtgga actgcaactt
     2581 atgacggaat ggctcttgct cagtccatca tcgaatatat ccatgagcac atcggagcta
     2641 agaccctctt tgcgacccac taccatgagt tgactagtct ggagtctagt ttacaacact
     2701 tggtcaatgt ccacgtggca actttggagc aggatgggca ggtcaccttc cttcacaaga
     2761 ttgaaccggg accagctgat aaatcctacg gtatccatgt tgccaagatt gctggcttgc
     2821 cagcagacct tttagcaagg gcggataaga ttttgactca gctagagaat caaggaacag
     2881 agagtcctcc tcccatgaga caaactagtg ctgtcactga acagatttca ctctttgata
     2941 gggcagaaga gcatcctatc ctagcagaat tagctaaact ggatgtgtat aatatgacac
     3001 ctatgcaggt tatgaatgtc ttagtagagt taaaacagaa actataaaac caagactcac
     3061 tagttaatct agctgtatca aggagacttc tttgacaatt ctccactttt ttgctagaat
     3121 aacatcacac aaacagaatg aaaaggagct gacgcattgt cgctcccttt tgtctatttt
     3181 ttaaggagaa agtatgctga ttcagaaaat aaaaacctac aagtggcagg ccttggcttc
     3241 gctcctgatg acaggcttga tggttgctag ttcacttctg caaccgcgtt atctgcagga
     3301 agtattagac gccctcctta ctgggaaata tgaagctatt tatagtatcg gggcttggtt
     3361 gattggtgtg gccgtagtcg gtctagttgc tggtggactc aatgttgtcc tcgcagccta
     3421 tattgcccaa ggagtttcat ccgaccttcg ggaggatgcc ttccgtaaaa ttcaaacctt
     3481 ttcttatgct gatattgaac aatttaatgc gggaaatcta gtcgttcgaa tgacaaatga
     3541 tatcaaccag attcagaacg ttgtcatgat gaccttccaa attcttttca gacttcccct
     3601 cttgttcatc ggttcgttta tcctagcggt tcaaacctta ccttctctgt ggtgggtgat
     3661 tgttctcatg gtagtcttga tttttggttt gactgctgtc atgatgggaa tgatggggcc
     3721 tcgttttgct aaatttcaaa cccttcttga gcgcatcaat gccattgcca aggaaaattt
     3781 acgtggcgtt cgtgtggtca agtcctttgt ccaagaaaaa gagcaatttg ctaagtttac
     3841 agaggtctca gacgagcttc ttggtcaaaa cctttacatt ggttatgcct tttcagtagt
     3901 ggaacccttt atgatgttgg ttggttacgg ggcggtcttc ctctctattt ggctggtcgc
     3961 gggaatggtt cagtcggatc cgtctgttgt tggttccatc gcttcttttg ttaattacct
     4021 aagccagatt atctttacca ttgttatggt tggatttttg ggaaattctg tcagccgtgc
     4081 catgatttcc atgcgtcgta ttcgagaaat tcttgacgca gagccagcta tgaccttcaa
     4141 ggatatccca gatgaagagt tggttggaag tcttagcttt gaaaatgtga cctttaccta
     4201 tccaatggac aaggaaccga tgctgaaaga tgtgagcttt actattgaac ctggtcaaat
     4261 ggttggtgta gttggagcga ctggtgcagg aaagtcaacc ttggctcaat tgattccacg
     4321 tctctttgat ccacaggacg gggccattaa aatcggtggc aaggatattc gagaagtgag
     4381 tgaaggaacc ctgcgtaaaa cagtttccat cgttctccaa cgtgccattc tttttagtgg
     4441 aacgattgca gataacttga gacaggggaa ggggaatgct actctatttg aaatggagcg
     4501 cgcagccaat attgcccagg ctagtgaatt cattcatcgt atggagaaaa cctttgaaag
     4561 tccagttgaa gaacggggaa ccaatttctc tggtggacaa aaacaaagga tgtcgattgc
     4621 gcgtgggatt gtcagcaatc cacgtattct gatttttgat gattcgacct cagccttgga
     4681 tgccaaatca gagcgcttgg tgcaagaagc tttgaataag gacttgaagg ggacgacaac
     4741 cattattatt gctcaaaaaa ttagctcggt tgtccatgca gacaagatct tggttctaaa
     4801 tcaaggacga ttgattggtc aaggcacgca tgcagacttg gttgccaaca atgccgttta
     4861 ccgtgaaatc tatgaaacac agaaatgaaa gacaaactat aagaaaagtc aatagtttta
     4921 tctaaactat ttcttatttc aatttgatga tttggcgatg attttagagc acggcaaaaa
     4981 gctattgaaa aagtccattt tttcaaaggt aatcctgtgt taatttcaga aattatatca
     5041 ctttttgttc gtcaaatggc agctcttttt taggatataa aacagggttc ggataagttt
     5101 ttttgcaagg tggatgatgg ctacattgta atgttttcct tattctaact tagtcttaag
     5161 ataggcctta aaagcaggtg aaaagcgagg gcatgctttg gcagcttgta tgagtaccta
     5221 ccgcagatga ggggaacccc gtttgaccat tcttcctgct aaatcaatct gacctgactg
     5281 ataaatagaa gaatccagtc cagcgaaagc ttgtaattga gcaggattat caaaggcatg
     5341 aatatttcga atctcggcta aaatgaccgc ccctaaacga ttctcaatcc cagtaaccgt
     5401 cgtgatgacc gagttgaact cagccatcaa gtcattgaca cattttttcg ccttgtcaat
     5461 gagcctcttg taatgtttga tgatttcatt acacgagata aaacgtctat gcgttatcaa
     5521 actcattacc aattaaaaca aaaagctgtg gttagatcct ttcggaaatc gtcaagcgat
     5581 tggaggaaat gaactaatcc acagcggctt attccaagta taccacttgg gctttggcag
     5641 tagctaactg cgctaaatat aatataagga ggagtaaaat gaagacagtt caattttttt
     5701 ggcattattt taaggtctac aagttctcat ttgtagttgt catcctgatg attgttctgg
     5761 cgacttttgc ccaagccctc tttccagtct tttctggaca agcggtgacg cagctagcca
     5821 atttagttca agcttatcaa aatggcaatc cagaacttgt atggcaaagc ctatcaggaa
     5881 tcatggtcaa tcttggcctg ctggttttgg ttctatttat ctctagtgta atatacatgt
     5941 gtctcatgac gcacgtgatt gcagaatcga ccaacgagat gcgcaaaggc ctctttggta
     6001 agcttgctca gttgacggtt tctttctttg accgtcgaca agatggcgat atcctgtctc
     6061 attttaccag tgatttggat aatatcctcc aagcctttaa cgaaagcttg attcaggtca
     6121 tgagcaatat tgttttatac attggtctga ttcttgtcat gttttcgaga aatgtgacgc
     6181 tggctctcat caccattgcc agcaccccat tggctttcct tatgctgatt ttcatcgtga
     6241 aaatggcacg caaatacacc aacctccagc agaaagaggt agggaagctc aacgcctata
     6301 tggatgagag catctcaggc caaaaagccg tgattgtgca aggaattcaa gaggatatga
     6361 tggcaggatt tcttgaacaa aatgagcgcg tgcgcaaggc aacctttaaa ggaagaatgt
     6421 tctcaggaat tcttttccct gtcatgaatg ggatgagcct gattaataca gccatcgtca
     6481 tctttgctgg ttcggctgta cttttgaatg ataagtctat tgaaacaagt acagccctag
     6541 gtttgattgt tatgtttgca caattttcac agcagtacta ccagcctatt atccaagttg
     6601 cagcgagttg gggaagcctt cagttggcct ttactggagc tgaacgaatt caggaaatgt
     6661 ttgatgcaga ggaggaaatc cgacctgaaa aggctccaac cttcactaag ttgcaagaaa
     6721 gtgttgaaat cagtcatatc gatttttcat acttgcctga taaacctatt ttgaaagatg
     6781 tcagcatttc tgcccctaaa ggccagatga cagcagttgt tgggccgaca ggttcaggaa
     6841 aaacgactat tatgaacctc atcaatcgct tttatgatgt tgatgctggt ggtatttatt
     6901 ttgatggtaa agacattcgt ggctatgact tagatagtct tagaagcaag gtgggaattg
     6961 tattgcaaga ttcggtcttg tttagcggaa cgattcgaga caatatccga tttggtgtgc
     7021 cagatgctag tcaggaaatg gttgaggtag cagcaaaagc aacccacatt cacgactata
     7081 tcgaaagttt gcctgataag tacgatactc ttattgatga tgaccagagc atcttttcaa
     7141 cagggcagaa gcaattgatt tcaatcgctc gaaccctgat gacagatcca gaagttctca
     7201 ttctcgatga agcaacttca aacgtagata cggtgacaga aagcaagatt cagcatgcca
     7261 tggaggtggt tgtagcaggt agaactagtt tcgtcattgc ccaccgcttg aaaaccattc
     7321 tcaatgcaga tcagattatt gtccttaaag atggagaagt cattgaacgt ggtaaccacc
     7381 atgaactttt gaagctaggt ggcttttatt cagaactcta tcacaatcaa tttgttttcg
     7441 aataagaaag aagttgtcct atgtgggcag ctttttcttg tccataaaaa atgtttatca
     7501 cagccttaaa aaaaacatat tagacgaaag tcattttgag tgatatgata ggactatcgt
     7561 tagcattcga aaggagaggc atcatggcta gaacggttgt aggagttact gcaaatctat
     7621 gtcccgtaga cgcagaaggc aaaatcattc attcatctgt atcttgtaga ttcgcagaga
     7681 tcattcgtca agtcggtggt ctccctttag tcattcctgt tggtgatgag tcagttgtac
     7741 gtgattatgt ggaaatgatt gacaaactca ttttgacagg aggccaaaat gttcatcctc
     7801 agttttatgg agagaaaaag accgtcgaga gcgatgatta caatctggtc cgtgacgaat
     7861 ttgaattggc actcttgaag gaagcgcttc gtcagaataa accaattatg gcaatctgtc
     7921 gcggtgtcca acttgtcaat gttgcctttg gtggaaccct caatcaagaa atcgaaggtc
     7981 actggcaagg actacctttc gggacatctc actctattga gacagtagaa ggaagcgtgg
     8041 tggctaagct atttgggaaa gaaagtcagg tcaattcagt ccatcgtcaa agcattaaag
     8101 atttggcacc caatttccgt gtaactgcta ttgattcaag agaccaaact attgaagcga
     8161 ttgagtctat cgacgagcac cgcattatcg gtttacagtg gcatccagag tttctggtta
     8221 atgaagaaga tggcaattta gaattatttg agtatttatt gaatgaactg ta
//
LOCUS       DAVKQL010000079         7900 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVKQL010000079 DAVKQL010000000
VERSION     DAVKQL010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7900
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.079"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(267..563)
                     /locus_tag="VW561_001899"
     CDS             complement(267..563)
                     /locus_tag="VW561_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HEU9944116.1"
                     /translation="MKSKRMEFHNKFLIVSAMLAVISWLSLGVVSFPMLAGTLGISTK
                     AAATVVNLISAYSTVTAVISIVGAITGVGSIGSGIAATVLYILKKKGAAKAALW"
     gene            complement(1106..1999)
                     /locus_tag="VW561_001900"
     CDS             complement(1106..1999)
                     /locus_tag="VW561_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9944117.1"
                     /translation="MDELKREIGYKIKTIRRSCGKTQIDICGDESELTIRQLARIENG
                     QALATLPKLLLIADKLGVSLQNIVDVKVIEIPKGFLKLKDELIHSQTYADKGRIERKE
                     AILEEIYENYYENLPEEEQLIVDVTQARFDIYGSSDVTYGLGLVEEYFQQLLKKKYFS
                     VNDLLIIELYFFCCAMGLEDKEHFEELAQKVLLCSEYEDKDSLVQMEKVLLSLFIQIQ
                     TEDSLIYIQTFEKIIAKTRHVFYRPHLFLLKAKYALFVDKNVAEAESFYEKAISLAEL
                     LDDQVLVQKILAEKQIDFPTT"
     gene            complement(2753..2983)
                     /gene="blpU"
                     /locus_tag="VW561_001901"
     CDS             complement(2753..2983)
                     /gene="blpU"
                     /locus_tag="VW561_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HEU9944118.1"
                     /translation="MNTKKMSQFEIMDTEILACVEGGGCNWGDFAKAGVGGGAARGLQ
                     LGIKTRTWQGAATGAAGGAILGGVAYAATCWW"
     gene            complement(3186..3476)
                     /locus_tag="VW561_001902"
                     /pseudo
     CDS             complement(3186..3476)
                     /locus_tag="VW561_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3522..3755)
                     /locus_tag="VW561_001903"
     CDS             complement(3522..3755)
                     /locus_tag="VW561_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU9944119.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            complement(3761..4753)
                     /gene="plsX"
                     /locus_tag="VW561_001904"
     CDS             complement(3761..4753)
                     /gene="plsX"
                     /locus_tag="VW561_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEU9944120.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVGIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTIDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLSGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            complement(4750..5520)
                     /gene="recO"
                     /locus_tag="VW561_001905"
     CDS             complement(4750..5520)
                     /gene="recO"
                     /locus_tag="VW561_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU9944121.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDERRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            complement(5517..6686)
                     /locus_tag="VW561_001906"
     CDS             complement(5517..6686)
                     /locus_tag="VW561_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9944122.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQAISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQVEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFASAAFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            6835..7845
                     /locus_tag="VW561_001907"
     CDS             6835..7845
                     /locus_tag="VW561_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEU9944123.1"
                     /translation="MSFLSKNGAGILVCLLISILSWYLGGFFPVIGAPVFAIFMGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVILLGFGLNISQVFAVGQSSLPVILSTISIALI
                     VAYLFQRFFALDTKLATLIGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNDGFALFAGTAVNDTSSVTATASAWDSLYQTNTLESATIVKLT
                     RTLAIIPITLFLSYWQSREQKNKQGLQLKKVFPLFILHFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIVMAMSAIGLKTNLVAMVKSSGKSILLGAICWIAIILTTLGMQTLIG
                     IF"
BASE COUNT         2317 a         1841 c         1241 g         2501 t
ORIGIN      
        1 gcaagaagat agagaaaaat atagtttttc cttatataga aagtatttat ttttttactt
       61 tggataggca gtttcatatt ataacgattc aaagtagcgt aatctactac caaaaaaagt
      121 aaaaataatt tcccttgaaa aggaagatag cttttaaaat agattgacaa acttatgata
      181 acaaaaaaca aaagggttat cccaataaaa ttaaaataac ctaaaatact ttcacattct
      241 tttttagaaa gtattttcat ctagttctac catagagctg ctttagcagc acctttcttt
      301 ttcaatatat aaagaactgt tgctgcaata cccgaaccaa tagaacctac tcctgtgatt
      361 gcacctacaa ttgaaattac tgctgtaact gtactataag cactaatcaa attgactaca
      421 gtagcagcag cttttgtcga aattcctaat gtcccagcca acataggaaa agaaacaaca
      481 cctaagctta accaactaat gactgctaac attgctgaaa caataagaaa tttattatga
      541 aattccattc ttttagattt cattttagtg tactcctttt gtatattatg tatgttttat
      601 tgagagttaa ttcgcacatg ttgtattcct aactctcttc aaatgttcgt ctatatttta
      661 tactactttt attaacatat ctataataaa taaattacta ctacattttc ctagattatc
      721 aaattacttt tatatcacta agactcaata aaagataatg tctatttaac ttttatacga
      781 ttactacctc ctttttagtt tacacttcct acatcataaa taaacatact caaccctcac
      841 aatctccaaa taactcttct atatcattga aaattgagtt tcatcctcta tactaatgta
      901 ggaaacctat taaaacattc ctttaatcca atataggaat accttatgcc attaatctct
      961 acctgatatt atagatagaa ccagactcct atacttaaaa ggacaaacaa cgttccaacc
     1021 ataataaact tcctttcttt ttggaatcgt tttctttatg agtttagtat aacaaaaaca
     1081 tgaaaaaaac aacatgactt agaagtcagg ttgttgggaa atctatttgt ttttctgcta
     1141 aaattttctg aactaatacc tgatcatcta gcaattctgc tagagaaatc gccttttcat
     1201 agaaactttc tgcctctgcc acattcttat ctacaaataa agcatatttc gcctttaaca
     1261 aaaatagatg aggtcggtaa aaaacatgtc ttgttttagc aataattttc tcaaatgttt
     1321 gaatatatat cagactatct tctgtctgaa tttggataaa taggctcaga agaacttttt
     1381 ccatctgaac taggctatcc ttatcttcat attctgaaca tagcaatact ttttgagcta
     1441 actcctcaaa atgttcttta tcttctaatc ccatagcaca acaaaagaaa tacaattcaa
     1501 taatcaacaa atcatttaca gaaaaatatt tcttttttaa taactgctgg aaatattctt
     1561 ccactaaccc taaaccgtag gtcacatctg aactaccata aatatcaaaa cgagcttgtg
     1621 tcacatccac aattaactgc tcttcctcag gcaaattctc ataatagttt tcatatattt
     1681 cctcaagtat agcttctttt cgttcgattc ttcccttatc agcgtaggtt tgagaatgaa
     1741 tcagctcatc tttgagtttt aggaatcctt ttggaatctc tataacctta acatccacaa
     1801 tattctgtaa tgatactcca agtttatccg caatcaataa taatttagga agtgttgcta
     1861 aagcttgccc attttcaata cgtgctagct gacgaatcgt caactctgat tcatcaccac
     1921 aaatatctat ctgagttttt ccacaagatc gcctaatagt tttgatttta taaccaattt
     1981 cacgtttcaa ctcatccata gtaatactcc ttaagttgat tacaaagatt tctttttctt
     2041 cctaattctt ttgtcaatca tatataaaac gataagcgaa aacagcggta gaacaatata
     2101 actcttttca ctcattatca gctgaaatcc cagggttgct agtatcttaa agatgaaaat
     2161 attcatcaaa aatagaaaaa caaatactaa aaaatatttt aaaaccttct tcatttcttt
     2221 tcttcaactt tcctaatttc tacacctatt ttatcgaaaa tatatataaa actccaaata
     2281 aacacttcaa ctgcactttt tttggcaaaa aatagacaga acaaacatcc tacctactaa
     2341 aaatatgata tgaaaggaaa atgatttttt actgacaccc taggcaagct tgggacaaat
     2401 aaatcattga tagcaacatt tacgacatcc tatttaaagt tccttcaatt tctaatgtcc
     2461 ccgctatttc taagccacct ctggtatgat ctattcctag tcagaataga taacaccgtt
     2521 ctaaattacg attcatagtt actccttttt tgactcgtta ctataagact cctatttaca
     2581 tgcgcaaaat aattttgatt cagattcatc atttatagtt ttgattattt tttaacagat
     2641 ttttctgttg tcttctccga agactttaaa aatttttttc taattaaatc atccatataa
     2701 ggaccaaata taccaactac taaaccaata ataaaacttt taaaatccat aattaccacc
     2761 aacatgtcgc tgcataggcc acacctccaa gtatagctcc tcccgcagca ccagttgctg
     2821 caccttgcca tgttcttgtt ttaattccta gctgaagacc tcgtgctgct cctcctccaa
     2881 cacctgcttt ggcaaaatct ccccaattgc atccgccacc ttcaacgcaa gcaagtatct
     2941 cagtatccat aatttcaaat tgtgacatct tttttgtatt cataacgaat acctcttttt
     3001 tatttttaat atttgtcttg ttacaaactt gacaagttta gtataacagt atttattaat
     3061 ttttttcatc caaatcttga attggcatcg aaacgtcttg aattagcttt tttgtttcaa
     3121 aatcatctct atttttaaaa aagatgtttt ctaatcactt ttttactatt agacttcctg
     3181 caaaactaga atcctagttc atgattgata ataccagcaa tcaaattcat tcgtaatccg
     3241 aaacgtttac gatgatttcg ataggttgtt gaaaacattt taaacgtttt tactttggca
     3301 aagatattct caaccttgct gctctcctta gatagcgcat gattacaggc tttatcttca
     3361 actgttagcg gcttgagttt gctggattta cgtggagttt gtgcttgagg atatatcttc
     3421 atgagccctt gataaccact gtcagccaag attttaccag cttgtccgat atttctcatt
     3481 tctaaaaacc atctacttcc gttgacttgg aactccgatt gctattttcc ttgaatgatt
     3541 ttaaccacat ctcctacgtt ttggagttgg tcaatttctt catcgctgat ttcgatacta
     3601 aattcatcct ccagcgtcaa gataaactcc atcaaatcaa ctgagtcagc atccaagtcg
     3661 tctttcagac tcaaggattc tgtcacgaca aagtcctctc cctgtcgctc ttggataatg
     3721 gtcacaatac ggtcaaaaat ttctttttct gtcatctctt ttattctcct gaaaattcac
     3781 gcgcagtctg ggcaaccacg tctgtttcta gcatggtacg gatctgacga atcgtactat
     3841 aaacagcctt ggcatcgctt gagccatgag tcttgacaac aggtgcctta acaccaaaca
     3901 agaccgctcc accaacatct gaataattga gctgtttttt caaaccactg aggctgtcct
     3961 tgagaaggag ggcacctagt ttcgctcgaa gaccaccacc tgtaatagct gtcttgagca
     4021 agcccatgat tcccatagct gtcccttcga tggatttgag cacagcgttt cccgtgaaac
     4081 catctgccac aacaacatct gcaacgccat tcatcaaatc acgcgcttcc acgtttccga
     4141 taaagttcaa actttcatca gccgccagta attcataagt ttccttacga agcgggtcgc
     4201 ccttgctact ctctgttccg ttgttgagca aaccaacgcg tggttgcgca atgccacgga
     4261 catttttagc atagaaggaa cctaggacag cgtattgatg gaggtgctgg gctgtatttt
     4321 ctgcattagc accgaggtca agcatgtcaa aacctttccc atctatagtc ggcaatgttg
     4381 acataagtcc aggacggtcg atattcttga tacgacccac gatgaagaat ccagcagcca
     4441 acaaagcacc tgtattccca gccgaaagga cagcgtctgc ttcaccatct ttgacagcct
     4501 tggctgccaa taccatactg gcatttttct tattccgaat agctctcgta ggttcatcat
     4561 ccgaatcaat cttctcatcc gtatggataa tgccgacgcg ctctgtcgct gtcagatatt
     4621 gcttgatttt agcttcatct ccgtagagtt gaacctcgat atctgaaaag tcagataggg
     4681 cttgattgac accctcaacg atggcctgag gtgcgtaatc gccccccatg gcatctactg
     4741 cgattttttt catttctttt cctcttttag taattgtccc cagtctgcta gggaatcaat
     4801 aaatttcttt gattttaggt gaatcccaac gtactcttca tatagttgat ccataaattg
     4861 gcgtagctct tgcttgattc caggcttgag cgaaatggtc tccaaagtct caaaatcaat
     4921 agcttgaaat tgattgagca gataggggat attgggattg agatggcaac gtctctcatc
     4981 ctcatgataa tgctctggac agaggcaggc tccatatttg aaagaaaagt caaaagcctg
     5041 accaacccga tggcagaaga cacactcatt aaaattgagg ctgattccaa atcgagtcaa
     5101 aatttgaatt tcaaaaatat tggtcaaaac ctgataatcc aagcctgctt ccatcaactc
     5161 caaagtcttt tgcaaaaaag caaacaaggg agcatcctgc tgattgtcct gcaaactagc
     5221 atctgcaaga gctgccacat aggtcgcata ggccatgaca aagaggtcac tattaatctt
     5281 gggaaaagtc atgacctcat gatagtcttc gatgtaactg agtccgtcat cattgattcg
     5341 caagagaaat cgtgccagca ccaagggctg aataacaggc gccagcttag actgaccagc
     5401 gtgtttgaca aaaaacatgc gtttgccaac ctgctctgta aaaattttga cgagcttatc
     5461 atcctcacga aaattgcgat tgtaaagcac caagccttga ctcgtgatag actgaatcat
     5521 gcttctctca tgtactcctc aagtcgtttc atggcttctt tgatagtctc catgctggct
     5581 gcataagata ggcggacgta gccttccccg taacgtccaa aggctgcacc agggataaag
     5641 gcaacggcct tcttctgagc aaaatccttc agaaaagcaa aggagtcttg attgtagccc
     5701 gctggaattt tagcaaaaat atagaaggca ccgtctggtt tgataatctc aaaaccaaga
     5761 gcagtcattt tttcgatgat atagtcccga cgctggatat attccttctt catgggctcc
     5821 gcatcatttt taccagccgt caaggcttct accgcagcat gttgcgccat ggtatttgcg
     5881 gcagtgacca agtattggtg actcttgatt aactgggctg tgaaggccgc agaagcgaaa
     5941 atcagcccca aacgccaacc tgtcatggca tgcgatttag acaaaccatt gataataata
     6001 gcctggtctc tcaacatcgt tcctagagac acatgggctt cgcctgtgta ggtcaattct
     6061 gagtaaacct catcacagac aacaaaaatt tcgtacttgc gtaaaacagc tgccaaggcc
     6121 tctacctgct ctcgactgta ggtaattcct gtcggattgg ctggatagtt gagaataacc
     6181 gccttgagct tatcaccctg ctccaaaatg gccttctcca acatctcagg agtcaagaca
     6241 aaaccatttt cagtcgtatc aatctcaaca atttctgccc caactaagtt aacaatcggt
     6301 tcatagcctg gataagcagg agctggcaaa agtaccttgt ctccctcttc caaaatagcc
     6361 gtcaaagtcg cagataaagc ctctgtcgcc ccaattgtaa ccaagatttc attttcagga
     6421 gcatagtcca gttggtactt ttccttaaca aagtcactag ctgcctgacg tagagtcagc
     6481 agaccactca tccctgtata gtaggattgg ttctgatcaa tcgctcgctt ggccgcctcc
     6541 ttgacatggt ctggcgttgt aaaatcaggt tcccccaagg tcaaacgcaa gaccccagga
     6601 atctccgaaa tagcctggtc aaactgacga atcaacgaaa cttgaatttt atctaactgt
     6661 ttattaaagc gcttagttaa gtccatagat acctcctact ttcaaaacgt atctctatta
     6721 tactacaaaa gccccccgac cgcaaaaata cattagagaa tagctttcca ctttctattt
     6781 tacttttcct ctttacaggt ctataatggt aacagattct atttggaggt ttttatgtca
     6841 tttctatcaa aaaatggagc aggcatcttg gtctgccttc tcatttccat cctatcttgg
     6901 tacttaggag gattcttccc tgtgattggc gcacccgttt ttgcgatttt tatgggcatg
     6961 ctcctacatc cctttctctc gtcttataaa caactggatg ctggattgac ctttagttcc
     7021 aagaaattgc tccagtatgc cgttatctta cttggttttg gtctcaatat ctctcaagtc
     7081 ttcgcagttg ggcaatcttc actccctgtc atcctttcca ccatttcaat agccttgatt
     7141 gttgcctacc tcttccagcg cttctttgcc ctggatacaa aactggctac cttgattgga
     7201 gtaggttctt ctatctgtgg gggttctgcc attgcagcga cagcgcctgt tatccatgct
     7261 aaggaaaagg aagtagccca agccatctcc gttatctttt tcttcaatgt cttggctgcg
     7321 ctcatctttc caaccctcgg cacctggctt catctatcca atgacggctt cgccctcttt
     7381 gcaggaactg cggtcaatga tacttcctct gtaaccgcca cagctagcgc ttgggacagt
     7441 ctttaccaga ccaataccct cgaatccgca accattgtca aactcacgcg caccctagct
     7501 atcatcccca tcactctctt tctctcctac tggcaaagtc gcgagcaaaa aaataagcaa
     7561 ggcttacaac tgaaaaaagt cttcccactt tttatccttc acttcatcct tgcatcgctc
     7621 ctaaccaccc ttctgacctc tctcggtgtg tctagtagtt tctttacccc tctcaaacaa
     7681 ctctctaaat tccttattgt catggccatg agtgctatcg gtctcaaaac caatctggtc
     7741 gctatggtca aatccagtgg aaaatccatt cttcttggag ccatctgctg gattgccatc
     7801 atcctcacca ctcttggtat gcagaccctt atcggcattt tctaatactc ttcgaaaatc
     7861 tcttcaaacc acgtcaacgt cgccttgccg tatatatgtt
//
LOCUS       DAVKQL010000080         7565 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVKQL010000080 DAVKQL010000000
VERSION     DAVKQL010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7565
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.080"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            <1..99
                     /locus_tag="VW561_001908"
     CDS             <1..99
                     /locus_tag="VW561_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEU9944124.1"
                     /translation="ISSTVLPDIKIKLPIDSRGTPDWNYMERYRDR"
     gene            65..379
                     /locus_tag="VW561_001909"
     CDS             65..379
                     /locus_tag="VW561_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944125.1"
                     /translation="MGITWKDIEIDKEREGEIDKKSTPIFYGEFKNVRLSKEEYKNLK
                     EKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEEDKDKLRQKGLNKKMNYDVGE
                     SL"
     gene            1063..1224
                     /locus_tag="VW561_001910"
     CDS             1063..1224
                     /locus_tag="VW561_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944126.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            1316..2164
                     /locus_tag="VW561_001911"
     CDS             1316..2164
                     /locus_tag="VW561_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU9944127.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGRIQKAREGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQLEQDEYLISEGIHEAI
                     VSDEVW"
     gene            2210..2995
                     /locus_tag="VW561_001912"
     CDS             2210..2995
                     /locus_tag="VW561_001912"
                     /inference="COORDINATES: protein motif:HMM:NF024800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase zinc beta ribbon domain-containing
                     protein"
                     /protein_id="HEU9944128.1"
                     /translation="MNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKDGTKYKDFY
                     YYGCKHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEKINMKVDTS
                     EIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYRMYDKIDEL
                     ESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISALISEIQVYE
                     EKQSNGQWLKSITFKLPIIEENLNIGLDNDEQVECVSLLEKRS"
     gene            3263..3829
                     /locus_tag="VW561_001913"
     CDS             3263..3829
                     /locus_tag="VW561_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944129.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGVEV
                     EYFNPKNIPDYMEIIFNGNIVPLSRFMFDPGENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            4151..4339
                     /locus_tag="VW561_001914"
     CDS             4151..4339
                     /locus_tag="VW561_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEU9944130.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            4401..5159
                     /locus_tag="VW561_001915"
     CDS             4401..5159
                     /locus_tag="VW561_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU9944131.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEAVLYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVHDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLF
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVIF"
     gene            5217..6071
                     /locus_tag="VW561_001916"
     CDS             5217..6071
                     /locus_tag="VW561_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEU9944132.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGIWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            6183..7064
                     /locus_tag="VW561_001917"
     CDS             6183..7064
                     /locus_tag="VW561_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEU9944133.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKE"
     gene            7169..>7565
                     /locus_tag="VW561_001918"
     CDS             7169..>7565
                     /locus_tag="VW561_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944134.1"
                     /translation="MGLYKNQEKLEEKMKDKESYNKFIPKLYNEELLKNHFKETMCFS
                     RKELNNKSIINQKLIKFPIVGHEVWALFGKEKTGEWRCLQVGQSKNKVKAEIESLIEF
                     MSYDYNQLVDSIKDGVKNRNSTFYSNIYQS"
BASE COUNT         2799 a          987 c         1564 g         2215 t
ORIGIN      
        1 atatctagca cagtattgcc agatatcaaa attaaattac cgatagacag tagaggtact
       61 ccagattgga attacatgga aagatataga gatagataaa gagagagagg gagagataga
      121 taaaaagtcc acccccattt tttatggaga attcaaaaat gtaaggttaa gcaaagaaga
      181 atataaaaat ctaaaagaaa aattaaactc acacacagat ataatgataa ataaactatc
      241 cagatacatg gaaagcagtg gtaagaccta tcaaaaccac tatgtgacaa tcttaaaatg
      301 gtatgaagaa gataaagaca aactaagaca gaaaggttta aataaaaaaa tgaattatga
      361 tgtaggagaa tctttataga taaaaagagg tggaaaggca ctattaaaga ctttaagtct
      421 aaaaaggtat caaagtatgc cagagataaa aaaatataaa tatgacactt taaaggcgat
      481 tagaaaatta aagaaatgct agaagacttt tagatgacag ttaccaagag ggtctaattt
      541 tcactgctac ttatgcagtg ggaatttgac ctttttatat aagcaaattc tatttcaaaa
      601 ttggttacca agaaagactt cttttcactg ttaagtataa gagggtaatt tatagaattt
      661 tgttacatta agataaatat tttctgatgg tcaaatttta aaaagattaa taactctcga
      721 taaattttga accttaacaa ttgaatagac caagacaaga cgttaattat ttttggagcg
      781 taataaccta tccgtcaaga tctttcggct gaataattcg gcaaaaaaag ttctgctaag
      841 taaaaagaaa gtgagcgata agtaagagtt tgaatatagg gagggatacc ctatccgcta
      901 tgaagaaaat gaggataatg atacttctaa ggttgaagcc ggctcatccg gaatagaggc
      961 ggaggtgaga ttcctatgtt gctaatccaa agcacttgtc aatgtcaaaa aacttaatta
     1021 aacaaataat tattagatat aaaactttag aggaggtata tattggacaa tgactggaat
     1081 ggattggctg atttaatagc aaatctgatt gcaaaatatg ctggtgcttt agatttagat
     1141 aatctacccg atccaacgcc agcaaaaaat caagagatga aaaatagttt tgacatggca
     1201 aaaacacaaa ttgagacgga ctaataaaag tgttataata tacttgaaat gatagtccaa
     1261 acttaaatta aaaaaattat atatgatata gaaaaattat attgaggtta atactatgtc
     1321 aaaagaaaaa ataaaagtat acctctatac acgagtatct acgtcaatac agatagaggg
     1381 gtattcgtta gaggcacaaa aatcacgaat gaaagctttt gctatttaca acgattatga
     1441 aattgttgga gagtatgaag atgccgggaa gtctggcaag tctattgaag gtagaataca
     1501 gttcaatcga atgatggaag atataaaatc tggaaaagat ggagtatctt ttgttcttgt
     1561 gtttaagtta tcaagatttg caagaaatgc cgctgatgtt ctttctacac ttcagataat
     1621 gcaggattat ggagttaatc ttatctgcgt tgaagatggc attgattcat ctaaagatgc
     1681 tgggaaacta atgatttctg ttttatcagc tgtggctgaa atagaaagag aaaacatccg
     1741 tattcaaaca atggaaggtc gcattcaaaa agcaagggaa ggaaaatgga atggtggctt
     1801 tgctccgtat ggatataaac ttgaagacgg caagctgttt ataaatgagg aagaggcagt
     1861 tgcaataaga acgattttcg accagtatgt aaatactacg ataggagcca atgggatatc
     1921 taaatactta gagaatcatg gaattagaaa aatcccaaga cagaatggta agaatccttt
     1981 gtttgatgca ggtcttataa gaaagatatt aaagaatcct gtatataatg ggaaaatagc
     2041 atttggaaga agaactttag aaaaagttca tggtacaaga aatgaatata agcagcttga
     2101 acaagatgaa tatctaatat ctgaagggat acatgaagct atagtttccg atgaagtttg
     2161 gtaagctgct caggttaagc taaaatctca agcaaagaaa tatgagcatg tgaataaagg
     2221 aaaagataca cgcacacact tgctttctgg aattgtaaaa tgcccgatat gtggagtggg
     2281 aatgtttggg aacaagtgta tcaagaaaaa gaaagatggc acaaagtata aagattttta
     2341 ttactatggt tgtaaacata ggcagatgat aagaggtcat aagtgtacat tcagtaagca
     2401 aattagagaa gaattgttag atgatgctgt tgcagaggtg attgtcaaga tagtaagtaa
     2461 tccgaaattt gcttctatga tgcaagaaaa aatcaacatg aaggtggata cctctgaaat
     2521 agaaaaagaa atagataatt accaaaagga attgagaaag agtcattcta caaagtttaa
     2581 gctaattgag gaaatagata atttagatgt tgaagataag cattataaga gaaggaaaca
     2641 ggatttagat gatagactat atcgtatgta tgacaaaata gatgaattag aatcatcact
     2701 aattgatgcg aaagcaaaga aacagactat tgaagctgaa aagcttacag gagataacat
     2761 atataaggtt ctgatctatt ttgataagct ctataaagtc atgaatgatg tagagcgtag
     2821 gcagttaatt tcagctttga tttctgaaat tcaagtttat gaagaaaaac aatctaacgg
     2881 acaatggcta aaatccatta cttttaagct accaattata gaagaaaatc tcaacatagg
     2941 tttggacaat gatgaacaag ttgaatgtgt aagtttatta gagaaacgta gttaatcctc
     3001 aaaaggttcc ccgaaccttt tgagtcccac tgacgcatca cgctgaggcg gtatcgctgt
     3061 ttgtacgagt tgaagcatca gcaaagtaaa agtagatgtt cagccaatgc gacgaggtga
     3121 gaccgaaggt cgataaggtg tgttctgggt ataggactcg taaagtaagt aggaaatatg
     3181 atgagatgat acagagagcc tgaattggtc aggtgacgtg cttatttaca ttagcctgat
     3241 aatgaaaatg gagagattat cgatggttat attggaattg taccaaaatg attattctaa
     3301 agatttagta gcatttgatt ccatagaaga tgggaaagcc tttgtagctc aaatcccggg
     3361 atacactctg gaaacagaag atggtgttga agtggagtat tttaatccta aaaacatacc
     3421 ggattatatg gaaattattt ttaacggaaa tattgttcct ttatctagat ttatgttcga
     3481 tcctggggaa aatgtaaaca ttatttggaa agagatttca aatttatccc tcaaaaatga
     3541 tagagtgatc gaaggatatt caaaaattga tgcatatgtc gttaacaacc atgaagttaa
     3601 agcctatgtc gaaacaagag aaacgaacta tcgtaaagca aaagacttct tagaaagccg
     3661 cggatatgaa atcgacagaa gctttttcgg aagtgaagac ggtgaagcga ttctttacag
     3721 aaaaaaaggg atcgaagttt ggcatttctt gtgtcactta gacccaatgt ttgtagagat
     3781 tgaagatgta gaaggatatg ttaaagaaga agttggagag attcaatagt caaccaaagt
     3841 tccttgtgag tatatacctt tgctagtaaa agagtcaaaa cgttataacg aaggggggga
     3901 agaaaaccct tgagtgtaag tctttttctt gtttagaggg aaagtattag agtagccgct
     3961 cataatttat actagatttg ataactgatt tgagacagat tttatttcta taaatattgt
     4021 gaaaattttt taataccaaa gatttaaagt tactgtgtta aaaatcctct ccacttttaa
     4081 actaatgtat tataaaagcc agttgacaca agtgttacac ctgtgataga attaacttga
     4141 ggtgatgaaa atggcaaccg ataaaaatag aataatgatt agcttggatg ataaaaatct
     4201 agagaaactt gaaaatttag tagaagatgc tagagataga aggggaatgc gactaacaaa
     4261 gtcccaggtt attgaattac tcctaaatac cgttgattat ttcgatgata ttatgggggc
     4321 tatttactca aaaaaataag agcaaaattg ctattatttt ttacaaattg gtgtaacact
     4381 agtgatatag gagtgatgaa atggttgttt atattagaca aagtaaatta ccaagtgagg
     4441 tttcaattaa taaatacaat gctcaagtag gtgcctactt acaaggagaa gaagctgttt
     4501 tatatcaatc tttttctgaa ataaaagagt taacaagtga agatatagtt gtagattata
     4561 taatggagac tagagcatta ctaaaaatga tgggcttaaa cgttccggtt catgactatc
     4621 ctattgagct taaagagttt tatggtcgaa agatttatgc tggtatttta ggagagattg
     4681 tgaatatacc tgataattgg ggaaaattta ttaagcctaa agctggttca aaagtcttta
     4741 ctggaagagt tgttaatgga acccatgatt taataggtat tggtctacct ttcgactatc
     4801 ctatatggat tagtgaggtt gtagaattca tagctgaatg gcgttgtttt gtgttagatg
     4861 gtcgcgtatt agatgttcga ccctatacag gtgattatca tgcacaattt gatgcaagtg
     4921 taattgatga agcgatatca tgttggaaag atgcgccaat agcttatgga ctagatatcg
     4981 gtgttactcg cgatggcaga acacttgttg ttgaagtaaa tgatggttat gcattgggaa
     5041 attatggcct atttccttta aaatcaatca attttcatag agctaggtgg aaagaaatgg
     5101 taaaacccta ttttgaaaaa aatgaaattt ttaaaatcca acaggatgtt attttctaaa
     5161 ataaatatgg tagataacca atataaaaag catcaaaatg aacaaggagg aatctgatgc
     5221 ttggagcaat tattggagat attgtcggtt ctgtttacga atggaacaat atcaaaacaa
     5281 aggactttcc tttatttcgg aaggactgct tttttacaga tgacacggtt atgacctgtg
     5341 ctgttgcaga agcaattatg aatggtggac aaaaagacga cttcattgac gcgatgaaga
     5401 aatatggcag aatgtatccg aatgctgatt acggtgctcg gtttaatgca tggctaaaca
     5461 gcgataatcg tgagccttat aatagctttg ggaatggatc agctatgcgt atttctccat
     5521 gtgcttggat catggactgt ggtttttatg cgaaaactgg tatatggcca tcatctagag
     5581 gacttacgag tctttctgca gaggtaactc ataatcatcc agaaggtgtc aaaggcgcta
     5641 tggctacagc tgatgctatc tttctatgtc gtttttactt tggtggttat tgtagagagt
     5701 acgagcaatc aattaacgac aatcctacag agtgtaaaag acgaattaag gattatatag
     5761 aaaaagaata cgattacaat ctatctcaaa ctttagatga aatccgtcct aactatcgtt
     5821 ttaacgaaac atgtcaggaa actgtccctc aagccattat cgcctttctt gaaagtagag
     5881 acttcgaaga tgcgataaga aatgccatct cacttggtgg cgacagtgat actctcgcag
     5941 caatcacttg tagcatagca gaggcagctt acggtattcc tgattggatc aaggataagg
     6001 cctattctta cttggatgaa cccttaaagg atgtagttag gcgatgggaa aatagaataa
     6061 aagcgtatta atctccagta cgtcagattt ctcttttctg tattttcatt gtataatagg
     6121 attacattta cttgcatacg acgaaatgta aacatttctg tttaaaatta aggataaaat
     6181 caatgaaaaa agcaatggta attatcaacc ctacttctgg tggcgagaag gctttggatt
     6241 acaaagaaaa gctggagaat aaagcaaaag aatactttga atatgttgaa accaaaatta
     6301 ccgaaaaagc gctggatgca acacattttg ctgaagaagc ttctcgtgag cagtatgatg
     6361 cagtggttgt gtttggtgga gatggaactg tcaatgaagt catttcaggt attgatgaga
     6421 gagactacat tcctaagtta gggattatcc caggcggtac gggtaacctc attacaaaac
     6481 ttttggaaat caatcaagac atcgatggcg caattgacga actggatttt gatttaacca
     6541 ataagattga tatcggtaaa gcaaatgaca actattttgg ttatatcttt agtatcggtt
     6601 ctctgcctga ggcgattcac aatgttgaaa tagaggacaa aacaaaattc ggtattttaa
     6661 cctatgctgt aaataccatg aagtctgtca tgacagatca ggtctttaac attaaggttg
     6721 agacagaaaa tggaaattat gttggtgaag ctagccatgt tttggtcctt ttgacaaatt
     6781 acttcgctga taagaaaatc tttgaagaaa acaaggacgg ctatgccaac attttgattc
     6841 tgaaagatgc ctctatattc tccaaattat ccgtcattcc tgatttatta aaaggggatg
     6901 ttgtcgcaaa tgataatatc gagtatatca aagcgcgtaa tattaaaatc tcttcagata
     6961 gtgaattgga gtcagatgtt gacggagata aatcagataa cctacctgta gaaatcaaag
     7021 tcctagctca gcgagtagaa gtattttcaa aaccgaaaga gtaagtttgt cattcactga
     7081 gaaataaagt tttatatcaa cgaaagtagt tacttgtaag atttgctaaa ttttgtaaaa
     7141 ttaacatgtc gtttaaaata attggataat gggtttatat aaaaatcagg agaagttaga
     7201 agaaaaaatg aaagacaaag aaagttataa taaatttatt ccaaagttat acaatgaaga
     7261 acttttaaag aatcatttta aagaaactat gtgtttttct aggaaggaat taaataataa
     7321 gagtataata aatcaaaagc tcattaaatt ccccatagtt ggtcatgaag tttgggcatt
     7381 atttggtaaa gaaaaaacgg gcgaatggcg gtgtttacaa gttggacaaa gtaaaaataa
     7441 agtaaaggcc gaaattgaat cattaattga atttatgtct tatgattata atcaattagt
     7501 tgatagtatt aaagatggtg taaaaaatag aaatagtact ttttatagta atatttatca
     7561 atctt
//
LOCUS       DAVKQL010000081         6879 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVKQL010000081 DAVKQL010000000
VERSION     DAVKQL010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6879
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.081"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(<1..716)
                     /locus_tag="VW561_001919"
     CDS             complement(<1..716)
                     /locus_tag="VW561_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU9944135.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITANSL
                     CTFDFDNIKDDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQPNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEIDDDKAL
                     EDVARFLDDHFATNVNQGTL"
     gene            876..2630
                     /locus_tag="VW561_001920"
     CDS             876..2630
                     /locus_tag="VW561_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9944136.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISAALTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIVTPILVLVLGFIVSIRVGI
                     TLLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNY
                     NIEFENVSFAYNDKAVIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            2614..4335
                     /locus_tag="VW561_001921"
     CDS             2614..4335
                     /locus_tag="VW561_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9944137.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKMEDSERVHLKAEVTTILTLSISSIFSFISLAVVIFVGVNLIINKEINSLYLI
                     GYLLAAMKIKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDIDLKD
                     VEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQILIDGK
                     DIKEISTESLFDKVSIVFQDVVLFNQSIMENIRIGKQDASDEEVKRAAKLANCTDFIE
                     KMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNEKKIQES
                     LNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLIEKTK
                     MAEEFIY"
     gene            4346..4930
                     /locus_tag="VW561_001922"
     CDS             4346..4930
                     /locus_tag="VW561_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU9944138.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFEKAGIV"
     gene            4930..5625
                     /locus_tag="VW561_001923"
     CDS             4930..5625
                     /locus_tag="VW561_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9944139.1"
                     /translation="MPTFSSNAPFNPNPISKILVVFITGLTVMHSINIRFELAIVCLI
                     GILLYLNGYKKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIPIVIRMFILPFMA
                     ASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKNIKMAMRVRGISF
                     KNPVKYLEYVSMPLLIISSNISDDIAKAAETKAIENPIAKTRYIRVKIQLIDFVYVLA
                     VAGLIVGGLIWLK"
     gene            5613..>6879
                     /locus_tag="VW561_001924"
     CDS             5613..>6879
                     /locus_tag="VW561_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9944140.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGNGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDMLKIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDEPSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLIDKGKLK
                     KTYTRSEFLKLDKNELNALSLRDKELSKLKVPYLKEGGEYQIKNLSYKFTDDECLSLK
                     DISFKLGKIYGIIGSNGRGKSTLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVM
                     QDVNHQLFTDEVFNELRLGVKNFDEEKAKIILKDLGLDEFIERHPMSLSGGQKQRLAI
                     ASVMCKNSPFIFFDEPSSGMDYSNMIKISE"
BASE COUNT         2480 a          919 c         1235 g         2245 t
ORIGIN      
        1 tcaagcgtgc cttgatttac attcgttgca aaatgatcat ctaagaatct tgctacatct
       61 tcaagtgctt tatcatcatc aatttcaatt ttatattttt ttctatttaa gtatgtgtca
      121 attactatac ttatccattc atttgccttt gctttaaaga aaaaatcagc ggcaggagcg
      181 tccatcttac aatttaatat ttccattgcc actctttcta aggcctttgt aagtattatt
      241 tgattcggtt gaagcaaggt tgaataaaaa gattctggat taatgttaat agatgctaaa
      301 tgtttttcta ttagctcttt tttaaaacca atggaaacag caagataaca acaattctcg
      361 tgtaataaaa aaacaaaatc atcttttata ttatcaaaat caaaagtaca taaagagttt
      421 gcggtaatag tttgatacgg attaaacttt tctccgtttg cactgacaat gtaacttgaa
      481 taaattgaaa catagtctga catactataa gtgctatttt gaactacttc ctctttgata
      541 taaaaatcat gtatatcgat aatgaagctg cctccttcat aaaaccaata taggccttct
      601 gcataagttg aattctcttt tttccaacaa aatgtatgac ctgcacttga atacttctca
      661 ttgtttttac attcatctac tttcatataa tcttttacaa aatcataata tgacataaca
      721 tactatccct tttagacaat attccaatta gccttattaa ttcaaaacta ttgtattagt
      781 aattataaca gatgtataat agaaaagcaa tgatagatat tatcaattca gcgaatttat
      841 atctaaaagg gatattaaag aaaggagata tgcttatgaa gatttacaaa aaactatttg
      901 cttatgtcca agataagaaa tatcttgggg ttttggccat aattttttct gctatatctg
      961 ctgcacttac agtatatgga tattatttaa tctacaaatt tctagataag ttaataatta
     1021 attcaaactt atccggtgca gagagtatag cattaaaatc tgttattaca ctaacaagtg
     1081 gagcgatatt ttattttgtc tcaggaatgt tttcacatat cttgggattt aggcttgaaa
     1141 caaatttaag aaaaagggga atcgatggtc tggaaaaagc aagttttagg ttctttgact
     1201 taaatccatc tggtcaaata agaaagatca tagatgataa tgcagcacaa actcatcagg
     1261 tggtagctca catgattcca gatagctctc aggcaatagt cacacctata cttgtccttg
     1321 tactaggctt tatagtaagt ataagggttg gtataacttt gcttgctctt actataattg
     1381 gcggcttaat tttaggggca atgatgggcg agcaagaatt tatgaagata taccaagaat
     1441 ccctatctaa actaagtgct gaaactgttg agtacgtgag aggaatgcaa gttgtaaaaa
     1501 tatttaaagc aaatgtagag tcatttaaaa gcttttacaa ggctataaaa gattactcaa
     1561 agtatgctta tgattattcc ctatcttgta aaaggcctta tgttttgtat caatggttat
     1621 tttttggact gattgcaatt ttaattattc ctatagttta ttttatgact agcttagcta
     1681 gcgcaaaggt gattttactt gagcttatca tgattttatt tttatcagga gttctctttg
     1741 tttcattcat gagaatgatg tggtactcca tgtatatttc tcaaggaaat tatgcagtag
     1801 atactttaga ggcgctttac gaagatatgc aaaaagacaa attagtgcat ggtaatgtca
     1861 ataattttaa aaactataat atagaatttg agaatgttag ctttgcttat aatgataaag
     1921 ctgtcattga aaatttatcc tttaatttag aagaaggaaa gtcctacgca cttgtcggtt
     1981 catctggatc aggcaaatca acagtagcaa aacttatatc aggtttttac aatgttaata
     2041 aaggaagcat aaagataggc gggatagcaa taagtgaata ttctgacgaa gccttaatta
     2101 aagccatttc ctttgttttt caagattcaa aattatttaa gaagagcatt tatgataatg
     2161 tagctttagc taataaagat gcgacgaaag atgacgttat gagagcctta aaattagcag
     2221 gatgcgattt aatattagac aaattcccag aaagagaaaa tacaatcata ggctcaaaag
     2281 gtgtttattt atccggtgga gaaaaacaaa gaattgcaat tgctagagca attttaaagg
     2341 attccaaaat tattattatg gatgaagcat cagcatctat tgacccagat aacgagtttg
     2401 aattgcaaaa agcttttaaa aatcttatga aggataaaac agttatcatg attgcacaca
     2461 ggctatctac aattaaagac cttgatgaaa ttattgtcat ggatagtgga aaaattatag
     2521 aaagagggtc tgacaaagaa ttaatgtcaa aagatacaag gtataagagc ctgcaagaga
     2581 tgtttaacag tgcgaatgaa tggagggttt caaatgaaag agttttataa aaaaagattt
     2641 gctcttacag atggaggagc aagaaattta agtaaagcaa cactggcttc atttttcgtt
     2701 tattgtataa acatgcttcc tgccatatta cttatgattt ttgctcagga agttttggaa
     2761 aatatgggca aaagcaatgg cttttatata gtattctcag ttttgacttt gatagcaatg
     2821 tatattttgc tttctatcga atacgataaa ttatataaca caacctatca agaaagtgca
     2881 gatttaagaa taaggacagc ggagaattta tcaaaattac ctctatctta cttttctaaa
     2941 catgacattt ccgacatttc acaaacaatc atggctgata ttgaaggcat agagcatgca
     3001 atgagccact caataccaaa ggtgggcggc atggtactgt ttttcccatt aatatctgta
     3061 atgatgctag cgggcaatgt caagatgggt ttagctgtaa ttattccatc tattttaagc
     3121 tttatattta tacctttatc taaaaaatat caggttaatg gacagaatag atattatgat
     3181 gtcttaagaa aaaactcaga aagctttcaa gaaaatatcg aaatgcaaat ggagattaaa
     3241 gcatataatt tatcgaagga tattaaagat gacttatata aaaaaatgga agatagtgag
     3301 agagtacact taaaggcaga agtaactaca attttaactt tgtctatatc ttcaatattt
     3361 agctttatat ctcttgctgt tgtgatattt gtcggcgtaa atctaattat taataaagag
     3421 ataaattctc tctaccttat aggatattta ctagctgcta tgaagataaa agactcttta
     3481 gatgcatcta aagagggctt gatggaaata ttttatttat cgcccaaaat agaaagatta
     3541 aaagaaattc aaaatcaaga tttacaagaa ggcgatgact atagcttaaa aaaatttgat
     3601 attgatctaa aagatgttga gtttgcctac aataaagacg caaaagtttt aaatggtgta
     3661 agttttaaag ctaagcaggg agaggtcact gctttggtag gtgcaagtgg ctgcggtaaa
     3721 acaactatct tgaaacttat atcaagactt tatgattatg acaagggaca aatcttaatc
     3781 gatggcaaag atataaaaga aatatcaaca gaatctcttt ttgataaggt ctctatagtt
     3841 ttccaagatg tggttctctt taatcaaagc attatggaaa atattagaat cggaaagcaa
     3901 gatgcaagtg acgaagaggt taaaagagca gcaaaacttg caaattgcac agattttata
     3961 gaaaaaatgg ataaaggttt cgatacagtt attggtgaga acggagctga gctatcagga
     4021 ggagaaagac aaagattatc aatagccaga gccttcttaa aagatgcgcc gatattgatc
     4081 ttagatgaga taacagcaag ccttgatgtt aacaacgaga aaaagattca agagtcttta
     4141 aataatttag ttaaagataa aactgttgta atcatttcac atagaatgaa atccatagaa
     4201 aatgcagaca agatagtagt tcttcaaaac ggaagagtag aaagcgaagg taagcatgaa
     4261 gagcttttac aaaaatcaaa aatttacaaa aatttaatag aaaagacaaa aatggcagaa
     4321 gaatttattt attaggagga ctacaatgga taataaaaaa ttaaaagtaa aagatttagt
     4381 aagtatcggt gtttttggcg taatttattt tgccttcatg tttggagttg gtatgatggg
     4441 cttgattcca atattgttct taatataccc gacagtatta gccatagttg caggaactgt
     4501 tgttatgtta tttatggcta aggttcaaaa gccatgggca ctatttatat ttggtatgat
     4561 atcaccactt gtgatgtttg cagctggtca tacctacgta gttgtggttt tatcacttat
     4621 agtaatgata atagcagaat taattagaaa gattggtaat tataattcat ttaaatacaa
     4681 tatgctttct tatgcaatct tcagcacatg gatatgtagc tctttaatgc aaatgctttt
     4741 agcaaaagaa aaatatatgg agtggtcttt gatgacgatg ggaaaagatt atgttgatgt
     4801 attagaaaag ttaataactt atcctcacat ggctttagta gccttaggtg ctttcttagg
     4861 aggaattctt ggagcatata taggcaaggc tctattgaaa aaacactttg aaaaagcagg
     4921 cattgtataa tgcctacttt ttcttcaaat gctcccttta atcccaatcc gatctctaag
     4981 atattagtcg tatttatcac aggcttaact gttatgcata gcataaacat ccgttttgag
     5041 ctggcaattg tttgtcttat tggtattttg ctttatttga atggatacaa aaaaacactt
     5101 ttcaaatgga ttatattgtg tgggatactt tactccttgc ctaattttat ggtgttatct
     5161 gaattaaacc ctatagttaa gatgtttttg agtataccta ttgttattag aatgtttatt
     5221 ttaccattta tggcagcaag ctttatgata aagacctcgg atgtaggtgc aataatttca
     5281 tcgatggata agcttaagat ttcaaagaat gtatccatac ctattgcggt tatgtttaga
     5341 ttcttcccat cttttaagga ggagaagaaa aacatcaaaa tggctatgag agtaagaggg
     5401 ataagtttta aaaacccagt caaatatctt gaatatgttt ctatgccact actcattata
     5461 tcatctaata tatcagatga cattgcaaaa gcggcagaaa caaaggcaat agaaaatcca
     5521 attgccaaga ccagatacat tcgcgtaaag atacagctaa ttgattttgt ttatgtttta
     5581 gcggttgctg gacttattgt gggaggctta atatggttga aataaaaaat ttaagtcttg
     5641 attatggtga agagcatata ttagatgata tatcactatc catagccgag ggagagtgcg
     5701 tgctatttac aggaaaaagt ggaaatggta agtcatcttt aataaattca atcaatggac
     5761 tagctgtaag gtatgataac gcaaagacaa agggcgaaat aattattgat ggtaagaata
     5821 taaaaaattt ggaactttat caaatctcaa tgcttgtttc aactgttttt caaaatccta
     5881 agacatattt ttttaatgtc aatacgacat tagaattatt attttatttg gaaaatatcg
     5941 gtcttgcaag agaagagatg gacaggcgtt tgaaggatat gcttaagata ttcccgataa
     6001 aaaatctttt gaacagaaat atatttaatc tatccggcgg tgaaaaacaa attctttgca
     6061 ttgcagcttc ttatatagca ggtacaaaga ttatagttat ggatgagcct tcatcgaatt
     6121 tagatattaa aagcataagt gttttggcaa agatgctaaa gatattaaaa gagaaaggca
     6181 taagcataat tgttgcagag catagaattt attatttgat ggacatagtt gaccgtgtat
     6241 ttttaataga taaaggaaag cttaaaaaaa cttatactag aagtgaattt ttaaagctag
     6301 ataaaaatga attaaatgct ttaagtttaa gagataaaga attaagtaaa ttaaaagttc
     6361 cttatttaaa agaaggtgga gagtatcaga taaaaaatct tagctacaaa tttactgatg
     6421 atgagtgttt aagcttaaaa gatatttcat tcaagcttgg gaaaatttat ggcataatag
     6481 gatccaacgg acgaggaaaa tcaacgcttt taagatgttt aataggtctt gagaaaaaat
     6541 caaaagaaga aatttatttt aagggagaga agctatctaa aaaagaaaga ctcaaaaact
     6601 cttcacttgt tatgcaagat gtaaatcatc aattattcac agatgaagta ttcaacgagc
     6661 ttagattagg agtaaagaat tttgatgaag aaaaggcgaa aatcatttta aaagatttag
     6721 gcctggacga atttattgaa aggcatccga tgagtttatc aggagggcaa aagcaaaggc
     6781 ttgcaatagc atctgttatg tgcaagaatt ctccatttat cttttttgac gaaccttcaa
     6841 gtggtatgga ttattccaat atgataaaaa tatctgaac
//
LOCUS       DAVKQL010000082         6804 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVKQL010000082 DAVKQL010000000
VERSION     DAVKQL010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6804
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.082"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(<1..716)
                     /locus_tag="VW561_001925"
     CDS             complement(<1..716)
                     /locus_tag="VW561_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9944141.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIR"
     gene            complement(713..2038)
                     /locus_tag="VW561_001926"
     CDS             complement(713..2038)
                     /locus_tag="VW561_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9944142.1"
                     /translation="MDLFGFGTVIVHFLIISHSYHFICKGQINRKELFVFGAYTLLTE
                     IVFDFPLYILYLDGLGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDEDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            complement(2059..2184)
                     /gene="comC"
                     /locus_tag="VW561_001927"
     CDS             complement(2059..2184)
                     /gene="comC"
                     /locus_tag="VW561_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEU9944143.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRLSKFFRDFILQRKK"
     gene            complement(2349..2422)
                     /locus_tag="VW561_001928"
     tRNA            complement(2349..2422)
                     /locus_tag="VW561_001928"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2386..2388),aa:Arg,seq:tct)
     gene            complement(2466..2945)
                     /gene="rlmH"
                     /locus_tag="VW561_001929"
     CDS             complement(2466..2945)
                     /gene="rlmH"
                     /locus_tag="VW561_001929"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEU9944144.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            3128..4309
                     /locus_tag="VW561_001930"
     CDS             3128..4309
                     /locus_tag="VW561_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU9944145.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLRSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            4367..5125
                     /locus_tag="VW561_001931"
     CDS             4367..5125
                     /locus_tag="VW561_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU9944146.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            5338..6699
                     /gene="dnaA"
                     /locus_tag="VW561_001932"
     CDS             5338..6699
                     /gene="dnaA"
                     /locus_tag="VW561_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU9944147.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYNYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
BASE COUNT         2360 a         1220 c         1073 g         2151 t
ORIGIN      
        1 tttctaattt ttaatcttga aataggacaa cgatggtctt catagaaaac aatttctaag
       61 ttttttcgat caatttctct gatattgcct atatttacca aaaatgactt gtgaggagaa
      121 taaaatcgct gagtatgttt gtccttttcc tgaatatctg tcatggtacc ataaaactct
      181 tttgcaaaat tcttaccaat aatgcgcaat ttatgagata cccctgttgt ttcaatatac
      241 aaaatatcat ggtaaggaat ttttaaatca tttcccttgt aattgtagtc gaaataatct
      301 acaacatctt cattttcaag taacatactc ttcgtgtaga agatattttg ctcaattctc
      361 ttcttaaaca tctcatcatt gatatcctta tcaacaaaat ctagggctga tacctggtat
      421 ttataggtta gagtcgcaaa ctctgatcga ctagtgataa agacgataat agcgtaagga
      481 ttgtaatgac gaatgagctg agccacttca aatccctttt tctcaattcc atgaatatcg
      541 atatctagga aataaagctg atttacttca tcattttcaa tgtattcttc aaattcacgg
      601 acttttcccg ttgtcttgta tgatattgga atattcgatt ctttcgaaat ttcatccaat
      661 attctctcta gtctcacttg atgttcaata acatcttcta aaattaaaac tttcattcaa
      721 attccctctt aaatctaatg atttgtctaa atgtactgcc ttccatctct gtttctaaaa
      781 taatattgtt gtacttatct agtagttctt tcacattatt taatccgact ccgcgatttc
      841 ttcccttagt ggagaatcct aaggcaaata gatctcctga aggagtcatc gtcattttac
      901 atgaattctg aatcacaata actgtttcag tttccatctt aataactgct acttccatct
      961 gctttttata gctatcagcc gatccttcga cagcattatt caataaaacg ctcatgatac
     1021 gaaccaaatc caatagttca attggaagct tggtaatcgt atcttttact tccagtgtaa
     1081 actctacacc attatttcga gcatagacaa ttgactgagc aaccaaactt cgtaaagctg
     1141 agtcttctat gttgttcaaa tcaaagtaag tgtacttatc tgaacgcaat ttatgatttg
     1201 ctttgactaa aacttcattg taaattctgt caatttcctg taaattacca ctgtcaattg
     1261 ccatctgcat gctgacaagc attccagcat aatcatgtcg aaaaccacgg atttcattat
     1321 acagaccaac aatttcatct gtgtaattct gtaaatgttt ctgttcaaat ttcttctgct
     1381 tcaaagcaat ctctttctcc atttgaactt tatgagaatt cattgcaaag aaggtcaaaa
     1441 ggagagagat aaagacaata gatgacaaaa tacttccaaa actattcaaa tgtttaatcg
     1501 tacttaccat atctgaaacg aaagatacaa tatgtagcaa tagtaaagca aaaaatactt
     1561 ttttcaagaa aggataaagg tagtcctcgt caaaataggc tagttccaaa tggaaatagt
     1621 aaatgatttt taatgtaaca aaataggtta acaccgtcac aacgaaaaag aatgggaaat
     1681 gatattgtaa aacaaaatta tctcctgtta tagaggagaa agttacggac agaaagttat
     1741 gagtgctctc atataaaaga gatagtagta aacttaggaa tagtcctcta tccctctcat
     1801 actgtttcat ccatcgaaaa taggaatata atcccaaagg aaataaaaat ctttcaatcc
     1861 ctaacccatc taaatataga atatataagg gaaaatcaaa tactatttct gttagtaatg
     1921 tataagcacc aaaaacgaat aattcttttc tatttatttg acctttacaa ataaaatggt
     1981 aactgtgact aataattaaa aaatgaacaa taaccgtccc aaatccaaat aaatccatta
     2041 ctctttcccc ttatttcatt acttttttct ttgtaaaata aaatcacgga agaattttga
     2101 caacctcatc tccccacctt taatcttttg taagtctttt tccttcaaag ctacaaactg
     2161 ttccaattta actgtgtttt tcataataaa atctcctaaa atgttttttc ttgtaagcta
     2221 acttacaaaa accattatac aaaatggaat ttcgttttag ataaaattct ctcaactgtc
     2281 atttttttct cccaaagtgt acttttttaa gaaaaaagcc gggaaaattc ccagctttgc
     2341 tattatattg atcccagcag gattcgaacc tgcgaccgtt cgcttagaag gcgaatgctc
     2401 tatccagctg agctatgaga cctaatacaa ttattctacc aaaaattcaa ttaaaagtca
     2461 attttctatt tatggtaggg gaatccctgc tgaatcgtaa aagcgcgata gatttgttca
     2521 acaagaacta gtctcattaa ctgatggggt aaggttaggc gaccaaaact gacagaaaga
     2581 ttggctctat tttttacaga tgatgacaat cctaaacttc ccccaataat aaaagtaaga
     2641 gtagaaaatc cttttataga agtttcttct aactgcttac taaattcttc tgagaagaaa
     2701 gttttccctt caatggctaa cacaataacg aaatcacggt cagcaatttt tgataaaatt
     2761 ctctgacctt ctatttctaa aatcttttga ttttctgatt cactggcctt atctggtgtt
     2821 ttttcatctg ataactcaat catttcaaac ttagcaaatc tagaaattcg ttttgaatac
     2881 tctgcgatac catcttttaa atacttttct ttcagtttcc caactgttac aactttaatt
     2941 ttcatgactc tattctaaca tattctctat tttttcacat cttattcaca aaataaaaaa
     3001 tagatttcaa ttaagaaaat cacagtttca aaagagttat ccacagtttg tgtaaaactt
     3061 ttgtgtttaa gttataatta agctagtcag tttatacttt cagtaattca aacatatgga
     3121 ggcaaatatg aaacatctaa aaacatttta caaaaaatgg tttcaattat tagtcgttat
     3181 cgtcattagc ttttttagtg gagccttggg tagtttttca ataactcaac taactcaaaa
     3241 aagtagtgta aacaactcta acaacaatag tactattaca caaactgcct ataagaacga
     3301 aaattcaaca acacaggctg ttaacaaagt aaaagatgct gttgtttctg ttattactta
     3361 ttcggcaaac agacaaaata gcgtatttgg caatgatgat actgacacag attctcagcg
     3421 aatctctagt gaaggatctg gagttattta taaaaagaat gataaagaag cttacatcgt
     3481 caccaacaat cacgttataa atggcgccag caaagtagat attagattgt cagatgggac
     3541 taaagtacct ggagaaattg tcggagctga cactttctct gatattgctg tcgtcaaaat
     3601 ctcttcagaa aaagtgacaa cagtagctga gtttggtgat tctagtaagt taactgtagg
     3661 agaaactgct attgccatcg gtagcccgtt aggttctgaa tatgcaaata ctgtcactca
     3721 aggtatcgta tccagtctca atagaaatgt atccttaaga tcggaagatg gacaagctat
     3781 ttctacaaaa gccatccaaa ctgatactgc tattaaccca ggtaactctg gcggcccact
     3841 gatcaatatt caagggcagg ttatcggaat tacctcaagt aaaattgcta caaatggagg
     3901 aacatctgta gaaggtcttg gtttcgcaat tcctgcaaat gatgctatca atattattga
     3961 acagttagaa aaaaacggaa aagtgacgcg tccagctttg ggaatccaga tggttaattt
     4021 atctaatgtg agtacaagcg acatcagaag actcaatatt ccaagtaatg ttacatctgg
     4081 tgtagttgtt cgttcggtac aaagtaatat gcctgccaat ggtcaccttg aaaaatacga
     4141 tgtaattaca aaagtagatg acaaagagat tgcttcatca acagacttac aaagtgctct
     4201 ttacaaccat tctatcggag acaccattaa gataacctac tatcgtaacg ggaaagaaga
     4261 aactacctct atcaaactta acaagagttc aggtgattta gaatcttgat tgacatctat
     4321 gtaaagaaag ctttacataa gagaaaagat gtgttagtgt agaatcatgg aaaaatttga
     4381 aatgatttct atcacagata tacaaaaaaa tccctatcaa ccccgaaaag aatttgatag
     4441 agaaaaacta gatgaactag cacagtctat caaagaaaat ggggtcattc aaccgattat
     4501 tgttcgtcaa tctcctgtta ttggttatga aatccttgca ggagagagac gctatcgggc
     4561 ttcactttta gctggtctac ggtctatccc agctgttgtt aaacagattt cagaccaaga
     4621 gatgatggtc cagtccatta ttgaaaattt acagagagaa aatttaaacc caatagaaga
     4681 agcacgcgcc tatgaatctc tcgtagagaa aggattcacc catgctgaaa ttgcagataa
     4741 gatgggcaag tctcgtccat atatcagcaa ctccattcgt ttactttcct tgccagaaca
     4801 gattctttca gaagtagaaa atggcaaact atcacaagcc catgcgcgtt ccctagttgg
     4861 gttaaataag gaacaacaag actatttctt tcaacggatt atagaagaag atatttctgt
     4921 aaggaaatta gaagctcttc tgacagagaa aaaacaaaag aaacagcaaa aaactaatca
     4981 tttcatacaa aatgaagaaa aacagttaag aaaactactc ggattagatg tagaaattaa
     5041 actatctaaa aaagacagtg gaaaaatcat tatttctttt tcaaatcaag aagaatatag
     5101 tagaattatc aacagcctga aataaggctg ttcttttatt tttttatctc acaaggttat
     5161 ccactatgtt tttcgataaa aagcttaata aatcaataat ttcttctttt atccccaacc
     5221 tgtggataaa gtttggtaac attgtggatt atttttcaca gcttgtggaa aattcttgct
     5281 atctatggta aaatatctct agtattaaac ttttaaatag taaaggagga gaaaggattg
     5341 aaagaaaaac aattttggaa tcgtatatta gaatttgcac aagaaagact gactcgatcc
     5401 atgtatgatt tctatgctat tcaagctgaa ctcatcaagg tagaggaaaa tgttgccact
     5461 atatttctac ctcgctctga aatggaaatg gtctgggaaa aacaactaaa agatattatt
     5521 gtagtagctg gttttgaaat ttatgacgct gaaataactc cccactatat tttcaccaaa
     5581 cctcaagata cgactagctc acaagttgaa gaagctacaa atttaactct ttataactat
     5641 agtccaaagt tagtatctat tccttattca gatacgggat taaaagaaaa gtataccttt
     5701 gataacttta ttcaagggga tggaaatgtt tgggctgtat cagccgcttt agctgtctct
     5761 gaagatttgg ctctgaccta taaccctctt tttatctatg gaggaccagg ccttggtaag
     5821 actcacttat taaacgctat tggaaatgaa attctaaaaa atattcctaa tgcgcgtgtt
     5881 aaatatatcc ctgccgaaag ctttattaat gactttcttg atcacctaag acttggggaa
     5941 atggaaaagt ttaaaaagac ctatcgtagt cttgatcttt tgttaatcga tgatattcag
     6001 tcactcagcg gaaaaaaagt tgcaactcag gaagaatttt tcaatacctt taacgccctt
     6061 catgacaagc aaaaacagat tgtcctaacg agtgatcgta gtccaaaaca tctagaaggg
     6121 ctcgaggaga ggcttgtcac gcgttttagt tggggattga cacaaactat cacaccccct
     6181 gactttgaaa cacgtattgc cattttacaa agtaaaacgg aacatttagg ctacaatttc
     6241 caaagtgata ctctagaata cctagctggg caatttgatt caaatgttcg agatcttgag
     6301 ggagccatca acgacatcac tttaattgcc agagtaaaaa aaatcaagga tatcactatt
     6361 gatattgctg cagaagccat tagagcccgc aaacaagatg ttagccaaat gctcgtcatc
     6421 ccaattgata aaatccaaac tgaagttggt aacttttatg gtgttagtat caaagaaatg
     6481 aagggaagta gacgccttca aaatattgtt ttggcccgtc aagtagccat gtatttatct
     6541 agagaactaa cagataatag tcttccaaaa attgggaagg aatttggggg aaaagatcat
     6601 accacagtca ttcatgccca tgccaaaata aaatctttga ttgatcaaga cgataattta
     6661 cgtttagaaa ttgaatcaat caaaaagaaa atcaaataat ttgtggataa cttttagttt
     6721 tttatctttt ttatccacat tttttaaaca agctaaaaaa cttgatatga cttgtttaaa
     6781 ggctgttttc cacagatttc acag
//
LOCUS       DAVKQL010000083         6466 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVKQL010000083 DAVKQL010000000
VERSION     DAVKQL010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6466
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.083"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            131..1081
                     /locus_tag="VW561_001933"
     CDS             131..1081
                     /locus_tag="VW561_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU9944148.1"
                     /translation="MGKKRWARNGSESNDASYAQVVSLYDDTSISVSNNETDKVLTGS
                     LYTETNEQGLTIPISLLKNWNEQTGKNLTTNDLIGKSVSASIVESAAETSKIAQFQTK
                     IVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTEELQK
                     NKKYTVLSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRSKEIG
                     VMKAVGYQNRGVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSITKVFE
                     LNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVESLRYE"
     gene            1258..2265
                     /gene="gap"
                     /locus_tag="VW561_001934"
     CDS             1258..2265
                     /gene="gap"
                     /locus_tag="VW561_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU9944149.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFVKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2332..3168)
                     /locus_tag="VW561_001935"
     CDS             complement(2332..3168)
                     /locus_tag="VW561_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9944150.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGD
                     VCQLTFDEEDYSQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLARCKLKTGRTHQIR
                     VHLSHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKK
                     NG"
     gene            3294..5489
                     /locus_tag="VW561_001936"
     CDS             3294..5489
                     /locus_tag="VW561_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU9944151.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENISLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVVTVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTSGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5542..5694
                     /gene="rpmG"
                     /locus_tag="VW561_001937"
     CDS             5542..5694
                     /gene="rpmG"
                     /locus_tag="VW561_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9944152.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            5704..5880
                     /gene="secE"
                     /locus_tag="VW561_001938"
     CDS             5704..5880
                     /gene="secE"
                     /locus_tag="VW561_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEU9944153.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            5935..>6466
                     /gene="nusG"
                     /locus_tag="VW561_001939"
     CDS             5935..>6466
                     /gene="nusG"
                     /locus_tag="VW561_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEU9944154.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAE"
BASE COUNT         2009 a         1123 c         1406 g         1928 t
ORIGIN      
        1 aatacctttt acgaggctct tttgtcctgc tcttgtttca attgactata gttcacggat
       61 acagttggga aagaagctat taaatatttg gagagtcctt ttgggatgaa catgtcaaat
      121 attactatag atgggcaaga aaagatgggc aagaaatgga tctgagtcaa acgatgcttc
      181 ttatgctcaa gtagtgagtc tttatgatga tacaagtatc tctgttagta ataatgagac
      241 ggacaaagtg ttgactggat ccctctatac tgaaacaaat gaacagggat taacgattcc
      301 aatcagttta ctaaaaaatt ggaatgaaca gacaggaaaa aatttgacaa ctaatgatct
      361 tattggcaaa tcagtctcag ccagcattgt agaaagtgct gccgaaacta gtaagattgc
      421 tcaatttcaa acgaagattg tacgtgtaat caatgatgaa gatgacatgg aggacagcaa
      481 cagtttcatg ctgtctcatc aaatggaaac gattttgaaa gaggctggat ttacgaaagc
      541 tgtatcttat tttatcttgg aactcaaaga tccatcacag acaaaagtag taacagaaga
      601 attacagaaa aataagaagt atactgtgct ttctcaacag agagttcttg atattgtgat
      661 tacctttatt cgtgttattc agggattatt gattgtgctt tcatcacaag ctattgtggt
      721 agcagcggtt atgattggta tcattattta catcaatatc atgcaacgtt ccaaggaaat
      781 aggtgtcatg aaagcagttg gttatcagaa tcgtggtgtc aaaggaattt ttatttacga
      841 ggctatctgg attgtaggca tcgccttgct gctggcattt ttggttgcac aaggggtggg
      901 aagtttggcg aatgcgattg taagtcactt ttacccatcc atcactaagg tttttgaatt
      961 aaatctttta tctgttttag gaactctagt tttcgctcta ttacttggtt atgtctcagc
     1021 ctacttcccg gcgcgtaaga ttagtaaaat ggatcctgta gaatcgttac gctatgaata
     1081 ataagggatt ccttgattta cagtcttaaa aagaaactca catagaaaaa caatggccat
     1141 acttgcaatt caactcaaat agttgtataa taggtttgtt gaaaggtgat ttgtagtgat
     1201 tcaagttact cttttcacaa taaaaatttc atgattttca taaggaggaa atcactaatg
     1261 gtagttaaag ttggtattaa cggtttcgga cgtatcggtc gtcttgcttt ccgtcgtatc
     1321 caaaacgtag aaggtgttga agttacacgc atcaacgacc ttacagatcc agttatgctt
     1381 gcacacttgt tgaaatacga cacaactcaa ggtcgtttcg acggtactgt tgaagttaaa
     1441 gaaggtggat tcgaagttaa cggtaaattc gttaaagttt ctgctgaacg tgatccagag
     1501 caaatcgact gggctactga cggtgtagaa atcgttcttg aagctactgg tttctttgct
     1561 aagaaagaag cagctgaaaa acaccttaaa ggtggagcta aaaaagttgt tatcactgct
     1621 cctggtggaa acgacgttaa aacagttgta ttcaacacta accacgacgt tcttgacggt
     1681 actgaaacag ttatctcagg tgcttcatgt actacaaact gcttggctcc aatggctaaa
     1741 gctcttcaag ataactttgg tgttgttgaa ggattgatga ctactatcca cgcttacact
     1801 ggtgaccaaa tgatccttga cggaccacac cgtggtggtg accttcgccg tgctcgcgct
     1861 ggtgctgcaa acatcgttcc taactcaact ggtgctgcta aagctatcgg tcttgtaatc
     1921 ccagaattga acggtaaact tgacggatct gcacaacgcg ttccaactcc aactggatca
     1981 gttactgaat tggtagcagt tcttgaaaag aacgttactg ttgatgaagt gaacgcagct
     2041 atgaaagcag cttcaaacga atcatacggt tacacagaag atccaatcgt atcttcagat
     2101 atcgtaggta tgtcttacgg ttcattgttt gacgcaactc aaactaaagt tcttgacgtt
     2161 gacggtaaac aattggttaa agttgtatca tggtacgaca acgaaatgtc atacactgca
     2221 caacttgttc gtactcttga atacttcgca aaaattgcta aataattctt gagttgatag
     2281 aaagcaaggc tttgtggtct tgctttttat atggaaaaat ggatgacacg atcatccatt
     2341 cttttttaat tctttttcaa atgtatttga aagggtagtg aaagttagct tttctaaagt
     2401 aagtgggtgg gtaaaggaaa gtcggaaggc atgaagcata agccgacttg tctttgattt
     2461 actattatag agagggtcgc ccaggatagg aagattatga tgcgaaaggt gcacacgaat
     2521 ctgatgggtt cgccctgtct ttagcttgca acgagccaag gaagtcttgt ttgagaattg
     2581 ctttaatctg cttacatgcg tttcagcata ttgcccattt tttgcatcaa ctattctttt
     2641 tctacgatca tggcgatcac gtccaatttt gtctctgaaa acaagttctt ttctgttgat
     2701 atttccatca actagagccc aatattctct agaaatctct tttttctcca ataagcgatt
     2761 gagaatgggc aggataaaag gatttttggc aaagagaact aagccactgg tttccatgtc
     2821 cagacgatga acgacatagc aggtttggcc aacataggta ctgacatggt taagaagggc
     2881 aatttcgttt ggttgattac catgcgtttt catcccctct ggtttgttta caataatcaa
     2941 gtgttgatct tgataaactt cctgcactaa gtctgggttg ccccaaggga tcgtcttttg
     3001 ggaataatct tcctcgtcaa aagtcaactg gcaaacatct ccaggattta cgatttcgtt
     3061 ccagtggact tcttcttgat ttatcaaaat atgtttcttg attctcaaaa aatgacggat
     3121 ttttctaggg atgaggagtt gttcctcaag taattgcttt accgtcattt gaggtagaga
     3181 ggcgggtaat gtaaatgtga attgcataca gatattgtaa caaaaaaagc cctatttgga
     3241 taggaaatag ctaaattctt gtcttcctat gatgaagatg ataaaataaa cgcatgaaat
     3301 tagataaatt atttgagaaa tttctttctc tttttaaaaa agaaacaagt gaactagagg
     3361 actctgattc tactatctta cgtcgctctc gtagtgatcg aaaaaaatta gcccaagtag
     3421 gtccgattcg aaaattctgg cgtcgttatc atctaacaaa gattatcctt atactaggtt
     3481 tgagtgcagg attgctagtt ggaatctatt tgtttgctgt agccaagtcg accaatgtca
     3541 atgatttgca aaatgccttg aaaactcgga ctcttatttt tgaccgtgaa gaaaaagagg
     3601 ctggtgcctt gtctggtcaa aagggaacct atgttgagct gactgacatc agtaaaaact
     3661 tgcagaatgc tgttattgcg acagaagacc gttctttcta taaaaatgac gggattaact
     3721 atggccgttt cttcttggct attgtcactg ctggacgttc aggtggtggc tctaccatta
     3781 cccaacagct ggctaaaaac gcctatttat cgcaggatca aactgttgag agaaaagcga
     3841 aagaattttt ccttgcctta gaattaagca aaaaatatag taaggagcaa attctaacca
     3901 tgtaccttaa caacgcttat tttggaaatg gtgtgtgggg tgtagaagat gcgagtaaga
     3961 aatactttgg agtttctgca tcagaagtga gtctggatca agctgcgact ctggcaggga
     4021 tgctcaaggg gccggaactg tataatccct tgaattctgt agaagattct actaatcggc
     4081 gcgatactgt cttgcagaat atggttgcag caggatatat tgataaaaac caagaaaccg
     4141 aagctgctga agttgatatg acttcgcaat tgcacgataa gtatgaagga aaaatctcag
     4201 attaccgtta cccctcttat tttgatgcgg tggttaatga agctgtttcc aagtataatc
     4261 taacagagga agagattgtc aataatggct accgcattta cacagagctg gaccaaaact
     4321 accaagcaaa tatgcagatt gtttatgaaa acatatcgct atttccgagg gcagaggatg
     4381 gaacgtttgc tcaatcagga agtgtagctc tcgaaccgaa aacaggggga gttcgtggag
     4441 ttgtcggtca agttgctgac aatgataaaa ctggattccg gaatttcaac tatgcaaccc
     4501 aatcaaagcg tagtcctggt tctacaatta agcctttagt tgtttatacg ccagcagttg
     4561 aagcaggctg ggctttgaat aagcagttgg ataaccatac catgcagtat gacagctata
     4621 aggttgataa ctatgcaggg atcaaaacga gtcgagaagt tcctatgtat caagccttgg
     4681 cagaatcgct taatctacct gctgttgtca ctgttaatga tttgggtgtc gacaaggctt
     4741 ttgaggcagg cgaaaaattc ggactcaaca tggaaaaggt tgaccgtgtt cttggtgtcg
     4801 ccttgggaag cggtgttgaa accaaccctc ttcaaatggc tcaagcatac gccgcctttg
     4861 caaatgaagg tttaatgcct gaagctcatt ttattagtag aattgaaaat gctagtggac
     4921 aagttattgc gagtcataaa aattcacaaa aacgggtgat tgataagtct gtagctgaca
     4981 agatgaccag tatgatgttg gggactttca ccaacggtac cggtattagt tcatcgcctg
     5041 cagactatat tatggcaggg aaaactggaa caactgaagc agtttttaat ccggagtaca
     5101 caagtgacca gtgggtaatt ggttatactc cggatgtagt gattagccac tggcttggct
     5161 ttccgaccac tgatgaaaac cactatctag ctggctctac ttcaaacggt gcagctcatg
     5221 tctttagaaa cattgccaat accattttac cttatacgtc aggaagtacc tttacggttg
     5281 aaaatgctta taagcaaaat ggaattgcac cagccaatac aaaaagacaa gtacaaacca
     5341 atgataatag ccagacagat gataatttgt ctgatattcg agggcgtgcg caaagtctag
     5401 tagatgaggc tagccgggct atctcagatg cgaagattaa ggaaaaggct caaacaatat
     5461 gggattcgat agtcaatcta tttcgctaag atgcttgtca aagcctagct ttcttgttat
     5521 aatggataag atggaggcgt tatggcacta aaaaaagcaa gcctagcttg tgcggtttgt
     5581 ggttcgagaa actattcaat caagatcagc ggaaacccca agcctacacg actagaagta
     5641 aataaatttt gtaagcattg tggcaagtac actacacata gagaaacgag ataggagaga
     5701 gcgatgcgtt ttattggaga tatttttaga cttcttaaag acacaacgtg gccaactcgc
     5761 aaggaaagct ggagagattt tcgttctatc atggaataca cagctttctt tgtagtaatt
     5821 atttacattt ttgaccagtt gattgtttca ggtttgattc gatttattaa cattttttag
     5881 aagattagtg gagttaatta cactagaaat cttctattta tgaaaggaaa tatcatggat
     5941 agttttgata aaggatggtt tgttttacaa acttattctg gttatgaaaa taaggtgaaa
     6001 gaaaatctat tacaacgtgc acaaacctac aatatgttgg ataatattct acgcgttgaa
     6061 attccaacac aaacagtgca agttgaaaaa aatggaaaga gaaaagaagt agaagaaaat
     6121 cgctttccag gttatgttct tgtagaaatg gtcatgacag atgaagcttg gtttgttgtt
     6181 cgaaacacac caaatgttac aggatttgtc ggatcacacg ggaacagatc aaaaccaact
     6241 ccattattgg aacaagaaat tcgtgacatt ttggtatcta tgggacaaac tgttcaagaa
     6301 tttgatttcg atgttgagat tggtcaaact gtacgtatta ttgatggtgc ttttgcagac
     6361 tacactggta agattacaga aattgataat aacaaagtga aaatgattat ctctatgttt
     6421 ggtaatgaca cagttgcaga agtaaaccta aaccaaatcg cagaat
//
LOCUS       DAVKQL010000084         6440 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVKQL010000084 DAVKQL010000000
VERSION     DAVKQL010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6440
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.084"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            39..632
                     /locus_tag="VW561_001940"
     CDS             39..632
                     /locus_tag="VW561_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEU9944155.1"
                     /translation="MMPLLAKAAFQAGAKGIRANSVRDISEIKEEVDLPIIGIIKRDY
                     DGFEPFISATMKEIDELVSEGVDILALDCTNRSRPGYDNITDFIHDIKVKYPNQLLMA
                     DISTFEEGKVAAESGVDFVGTTLSGYTPYSPKKDNPDFELVERLVKELDVPVIAEGRI
                     STPEQARKMLDLGAYAVVVGGAITRPLEIAKKFIEVV"
     gene            633..1565
                     /locus_tag="VW561_001941"
     CDS             633..1565
                     /locus_tag="VW561_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9944156.1"
                     /translation="MNQEILKKSKIQLTVEHIQKYWILYLMMIPATVLLILFTYGPMY
                     GIIMAFQDFTVFKGYTGSSFVGLKHFQRLFSDPLFYRLFKNTFMVGVLDFLFSFPAPL
                     IFALILKEVRKVRFKSVVQSISYLPHFIPLVVMVGIIFELFGSYGIINSLLSSLGMEP
                     ISFFTKSEWFLPLYIGSGVWKTIGWGSIIYMGALTNIDSTLYEAADMDGANRWHKMWH
                     VTLPSLRPTVVTLFILNAGGIMQVGFEKVFLMSSPATYEVSDVLSTYVYRQGILNSDF
                     SYSAAVGLFNNIVALLFVLLANKIAKKLGEEGIV"
     gene            1576..2505
                     /locus_tag="VW561_001942"
     CDS             1576..2505
                     /locus_tag="VW561_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9944157.1"
                     /translation="MIFSKNTKKSNRDKETFVDYLFIFILIIITLLMLYPLWWVVMTS
                     LSEPVWLSKNVVKLFPLKIDITAYKFIFGDSKLWQAYGNSVLYAALSTIVSVAVCSMY
                     AYPLTIPEIRGKKFWNTLILIPMFFSGGMIPLYIQVANLGLLNTAWALVLPGAVSTYY
                     IILFRTQFKSIPMELRESARIDGASEFRIFWNLVLPLSKVIFMIIALYCIVGSWNSFT
                     PPLLYLNDDTKMPLSIYLRNLVVTNTTDTAELSLSAGNTYYQQIAAQGGGIGMRIAIK
                     MGTIVVSVIPIMMIYPFIQKHFVKGILLGSVKG"
     gene            2519..3958
                     /locus_tag="VW561_001943"
     CDS             2519..3958
                     /locus_tag="VW561_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944158.1"
                     /translation="MQNLKKYLLLDSGLVVGYTLENAQLKLNKLKKDTENNPLFGEEY
                     FAENPKLWEVRFDNSYPNVIYDKELKLYRCWYQTFVSDEASEETPLAERGEKEYIVKS
                     SRMTALCYAESKDGVKWEKPNLNLVKFKGSKDNNIVLMHAHGAGIMLDEQELDPKKRY
                     KLVSKLEFSPNLHYMVVVFSEDGIHFDEPIPWKKWNPAADSHNFPFRDPKTGKYAVIT
                     RTWANNVRLSAITFSDDFINWSEPKEILRGDGFEDQIYSMPVISYADIYLGFASMYHE
                     GDTLNENYDKVDLELKFSANLEQWDSVCKGDYLIERGGGVYPQNADFDASCIFTAAPI
                     IEDDKIWFYYMGGNGNHTGFRESSLARGYIKKDQFAYYTNKREGIPAKISTVSFQTYD
                     DEIYILADIPENGYIKVAVGTKYGNAYSDFGQEDISLVQVSDGKYKLEFNKKSILDLG
                     NSPVAFKFEIENAKLYGIEGSIQQHYLKY"
     gene            4027..5604
                     /locus_tag="VW561_001944"
     CDS             4027..5604
                     /locus_tag="VW561_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9944159.1"
                     /translation="MVKLNKVLKGSVAFLSASLLLAACGNNANSKPAEKTADGKTKIS
                     FLSTWQINDVRPPKDETLVGQWLEEQLGIDIDLVTIPGEGVSSKVNSLITSNQLPTVL
                     LTTGDKSDIAGINKLGKQGAFLDLSQHMDKLPNYKKYLEKNNALAQIEDDEKHIYAFT
                     KFFTDENIMYTTPILRKDLLVGSEFEDLSKIKTVDDYTKVLKYLTEKQGSPAFIQRNG
                     YEGFMKRVTPLWNLSHRTYYDYESDSYKHPVEQPQLKQFVEWLKELRKDNVLHPDWAV
                     MKDETWEGLLASNKGSFTVDRMNIIGDNNFDKSFDWQPLVYPKINGKRYLQPKTPYIS
                     DSGWVINANAPKEEIDKALEFFNFLYADENQEKLAIGFEGKTFTRENPNTQAGIKWLI
                     QIYGENAGNDKAELLFKHGNQAFTRVQTKLDIEAQPGTFPKVMYDQVDKIKKDLGGFR
                     PDTPVLSFTTEELDVLTKSEAGLNTFFDENIIKFIEGQRDMSEWDSFINEAKSKDLDK
                     VTKVYNDAYKRVKDRMK"
     gene            5664..>6440
                     /locus_tag="VW561_001945"
     CDS             5664..>6440
                     /locus_tag="VW561_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU9944160.1"
                     /translation="MYDVVIIGAGSSGALAAIASGRLGLKTCLIEKGSRIGGVPINTL
                     MGSFANLYFDDSGRVNASKIVGELIERIIKKGGTVFNSMEELMNVNDFYQITIPYQPE
                     VYESVLTDMLIESKVDILLNIEVNNIIYDSKNIKSLEILSNQEVKLIDSKVFIDATGN
                     ATIASASGADVYEKNSSYGCLMRVGGVDISKTIEHIEKTKQWNYIEGYNQWLEEVLAN
                     RKYRATGAVLKDPVSYDHAPMLSKDDHFMNDEKWNYIKERW"
BASE COUNT         2186 a          875 c         1250 g         2129 t
ORIGIN      
        1 cgttagaagg agagccactt tatactccaa atggaggagt gatgccgtta ttagcaaaag
       61 ccgcctttca agcaggcgct aaaggtattc gtgctaattc tgtccgcgat atttcagaaa
      121 taaaagaaga agttgactta ccaattatag gtatcataaa aagagattac gatggctttg
      181 aaccttttat ttcagcaact atgaaagaga ttgatgaatt agttagtgaa ggagtagaca
      241 ttttagcttt ggattgtact aatcgttcaa gaccaggtta tgataatatt actgacttta
      301 ttcatgatat taaagtcaag tatccaaacc aattattaat ggctgatatt tctacttttg
      361 aagaagggaa agtagctgct gaatcgggag tagattttgt gggtacgaca ttatctggtt
      421 atacaccata tagccctaaa aaagataatc ctgattttga gttagttgaa agattggtaa
      481 aagagcttga cgtaccagtt attgctgaag gaagaataag tactccggaa caagctagaa
      541 aaatgttaga tttaggtgct tacgcagtgg ttgttggagg agctatcact cgtcctttgg
      601 aaatagcgaa gaagtttatt gaggtagttt agatgaatca agaaatatta aaaaagagta
      661 agattcaatt gacagttgaa catattcaaa aatattggat tttatattta atgatgattc
      721 cagcaactgt tctactaatt ttatttacgt atggaccaat gtacggcatt attatggcct
      781 ttcaagactt tactgtcttc aagggatata ctggaagttc atttgtcggt ttaaaacatt
      841 ttcaaagatt gttctcagat cctttgtttt atagattgtt caagaatacg ttcatggtag
      901 gggttttaga ctttttattc tctttcccag ctccgcttat ttttgcactc atattaaaag
      961 aagtcagaaa agtaagattt aagagtgtag ttcaatctat tagttatctt cctcatttta
     1021 ttcccctagt ggttatggtt ggtattattt ttgaactatt tggttcatat ggtattatta
     1081 acagtttact ttcatcactt ggaatggagc caataagttt ctttacaaaa tctgaatggt
     1141 tcttgcctct ctacattggt tcaggagtat ggaaaacaat aggttggggt tctatcattt
     1201 atatgggagc attaaccaat atagattcaa ctttatatga agcagcagat atggacgggg
     1261 ctaatagatg gcacaaaatg tggcatgtaa ctctaccaag tttaagacct acagtagtta
     1321 ctttgtttat actaaatgct ggtggtatca tgcaagtagg atttgaaaag gtattcttga
     1381 tgtctagtcc tgcaacatat gaagtatctg atgtattatc tacttatgtt tatagacaag
     1441 gtattttaaa ttctgatttc agttactcag ctgcagtagg attatttaat aacatagtag
     1501 ctttgttatt tgttttatta gctaataaga ttgctaaaaa acttggcgaa gaaggaattg
     1561 tataaggggg gtaaaatgat tttttcaaaa aatacaaaaa agagtaatag agacaaagaa
     1621 accttcgttg attatctctt tatattcatt ttaataataa taaccttact tatgttgtat
     1681 ccattatggt gggtagtgat gacgtcactt tcagaaccag tttggctatc taaaaacgtt
     1741 gttaaattat ttcccttaaa aatagatatt actgcatata agtttatttt tggggattct
     1801 aaactatggc aagcatatgg taatagtgta ttgtatgctg cactatctac aattgttagt
     1861 gttgcggtat gtagtatgta tgcctatcca cttacaattc ctgaaattag gggtaaaaaa
     1921 ttctggaata ctcttatttt gattccaatg ttctttagtg gaggaatgat cccgctttat
     1981 atacaagttg caaacctcgg tttattgaat actgcttggg ctcttgtttt acctggtgct
     2041 gtttctacat attatataat tttgtttaga acacagttta aatcaatccc aatggagctt
     2101 cgtgagtctg ctagaattga tggggcaagt gaatttagaa ttttttggaa tttagtatta
     2161 ccattatcaa aagttatttt catgattata gcactttatt gcatagttgg ttcatggaat
     2221 agttttactc ctccattact ttatttgaat gacgacacta agatgccttt atctatttat
     2281 ttgagaaatt tagttgttac aaatacaact gatactgctg aattatcact ttctgcaggt
     2341 aatacatatt accaacaaat tgctgctcaa ggtggaggta ttggtatgcg tattgctatt
     2401 aagatgggta ctattgttgt tagtgttatt ccaattatga tgatttatcc atttattcag
     2461 aaacattttg ttaagggaat cctgctaggt tctgtgaaag gttaataagg taattatttt
     2521 gcaaaattta aaaaaatatc ttttattaga tagcggttta gtagttgggt atacattaga
     2581 aaatgctcaa ttaaaattaa ataagctaaa aaaagatact gaaaacaatc cactatttgg
     2641 tgaagagtat tttgccgaaa atcctaaatt atgggaagtt cgttttgata attcatatcc
     2701 aaacgttatc tatgataaag aattgaaatt atatagatgt tggtatcaaa catttgttag
     2761 tgatgaagca agcgaagaga ctcctttagc agaacgtgga gagaaggaat acattgtcaa
     2821 gtcatctaga atgacagcac tttgctatgc tgaaagtaaa gatggagtaa aatgggaaaa
     2881 accaaatctc aatctagtaa aatttaaagg ttcaaaagat aataatattg ttctgatgca
     2941 tgcacacggt gcaggtatca tgctagatga acaggagttg gatcctaaga aacgatataa
     3001 attagtttca aaattagaat tttctccgaa tcttcattac atggttgttg ttttttcaga
     3061 agacggtatt cattttgacg aacctattcc atggaaaaaa tggaatcctg cagcagatag
     3121 tcataatttt ccatttagag atccaaaaac tggtaaatac gctgttatta caagaacctg
     3181 ggcaaataac gtacgtctaa gtgcaattac tttcagtgat gattttatta attggtcaga
     3241 acctaaagaa atccttaggg gagatggatt tgaagatcaa atttattcta tgccagtaat
     3301 ttcatacgcg gatatctatt taggttttgc ttcaatgtac cacgaaggcg acactttaaa
     3361 tgaaaactat gataaagtag acttagaatt gaaattctct gctaatttgg aacaatggga
     3421 tagtgtatgt aagggcgact atctcattga aaggggaggt ggagtatacc cgcagaatgc
     3481 tgattttgat gctagttgta ttttcacggc tgctccaata attgaagatg ataagatttg
     3541 gttctactat atgggtggaa atggtaatca cactggcttc cgagaaagtt cattagctag
     3601 aggatatatt aagaaagacc aatttgctta ttatactaat aagagagaag gaattccagc
     3661 taaaatatct acagttagct tccaaactta cgatgatgaa atttatatct tagctgatat
     3721 tcctgaaaat ggttatatta aagttgcggt aggtactaaa tacggtaatg cttattcaga
     3781 ttttggtcag gaagatatct ctcttgttca agtttccgat ggtaagtata agttggaatt
     3841 taataaaaaa tctatcttag atttaggtaa ttctccagtt gcgttcaaat ttgaaatcga
     3901 gaatgcaaaa ttgtatggta ttgaaggtag tattcagcaa cactatctca aatattaacc
     3961 agaataattc gttaacaaat aatctctata ttagtggaga tagatatata aaatggaggt
     4021 cttattatgg taaagcttaa taaggtatta aaagggagtg ttgcattcct gagtgcaagt
     4081 ttattgctag cagcttgtgg caataacgct aattcaaagc ccgctgaaaa aacagctgat
     4141 ggtaaaacga aaatttcgtt tctatctaca tggcaaatta atgatgttcg tccgcctaaa
     4201 gatgaaacac ttgttggtca atggttagaa gaacagctag gtattgatat tgatcttgta
     4261 actattcctg gcgagggagt aagttctaaa gtaaattctc ttattactag taaccagtta
     4321 ccgactgttt tgttgacaac aggagataag agtgatatcg ctggtatcaa caaacttggt
     4381 aaacagggtg cattcttaga tttgtcacaa cacatggaca aactaccaaa ctataagaag
     4441 taccttgaaa aaaataatgc attagctcaa atagaagatg atgaaaaaca tatttatgct
     4501 ttcacaaaat tctttactga tgagaatatc atgtatacta caccaattct gagaaaagac
     4561 ttacttgttg gttcagaatt tgaagatctt tcaaaaatta aaactgttga tgactataca
     4621 aaagttttga aatatttgac tgaaaaacaa ggttctccag catttattca acgtaatggc
     4681 tatgaaggct tcatgaagcg agttacacca ttgtggaatt taagccatcg tacatactat
     4741 gactatgaat cagattcata taaacatcca gttgaacaac ctcaattgaa acaattcgtt
     4801 gaatggttga aagaattacg taaagataac gtacttcacc ctgactgggc agttatgaaa
     4861 gatgaaactt gggaaggtct attggcttct aacaagggtt cattcactgt tgaccgtatg
     4921 aatatcattg gagataataa ctttgataaa tcatttgatt ggcagccact tgtttatcca
     4981 aaaattaatg gaaaacgtta tttgcaaccg aaaacaccgt acatttctga ttcaggttgg
     5041 gtaatcaatg ccaatgcacc aaaagaagaa attgataaag cgttagaatt ctttaatttc
     5101 ttatacgctg atgaaaatca agaaaaactt gctattggat ttgaaggtaa aacatttaca
     5161 cgtgaaaacc ctaatactca agcaggtatt aaatggttga ttcaaattta cggtgaaaat
     5221 gctggaaatg ataaagcaga actgctgttt aaacatggta accaagcatt tactcgcgtt
     5281 caaactaaat tagatattga ggcacagcca ggaacattcc ctaaagttat gtatgatcaa
     5341 gtagataaaa ttaagaaaga tttaggtggt tttagaccag atactccggt tctttccttt
     5401 acaactgaag aattagatgt tttaactaaa tctgaagcag gattaaatac tttctttgat
     5461 gaaaatatta ttaaattcat tgaaggacaa agagatatga gtgagtggga ttcatttatc
     5521 aatgaagcta aatctaaaga cttggataaa gttacaaaag tatataatga tgcctacaaa
     5581 cgtgtcaaag atagaatgaa atagactaaa tggtagctct ctggtttttc agagagctac
     5641 tactgcctga gggggagtaa gctttgtatg atgtagtaat tataggtgca ggaagttcgg
     5701 gcgcattggc tgcaattgct tcggggcgtc tagggttaaa aacttgtcta atagagaagg
     5761 gaagtagaat tggtggagtt cctattaata cactaatggg gtcatttgct aatctatatt
     5821 ttgatgattc tggtagagta aatgcaagta aaattgttgg ggaattaata gaacgtatta
     5881 tcaaaaaagg tggtactgtc tttaactcta tggaagaatt gatgaatgta aatgattttt
     5941 atcagattac aattccctat caaccagaag tttatgaatc ggttttaact gatatgttga
     6001 ttgagtctaa agttgatatt ttactaaata ttgaagttaa taatattatt tatgatagta
     6061 aaaatattaa aagtcttgaa attttaagta atcaggaagt taaattgatt gattctaaag
     6121 tttttattga tgctacaggg aatgcaacaa ttgcttctgc tagtggagct gatgtttatg
     6181 aaaaaaattc ttcttacggt tgtttaatga gagttggagg agttgatatc tcaaagacta
     6241 tagaacatat tgaaaaaaca aagcagtgga actatataga aggatataat cagtggttag
     6301 aagaagtatt agcaaatcga aagtacagag ccactggagc agttttaaaa gatccagttt
     6361 cttatgatca tgcccctatg ctttcaaaag acgaccattt tatgaatgat gaaaaatgga
     6421 attacattaa agaaagatgg
//
LOCUS       DAVKQL010000085         6262 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVKQL010000085 DAVKQL010000000
VERSION     DAVKQL010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6262
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.085"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            104..1483
                     /gene="glmU"
                     /locus_tag="VW561_001946"
     CDS             104..1483
                     /gene="glmU"
                     /locus_tag="VW561_001946"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEU9944161.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            1493..2038
                     /locus_tag="VW561_001947"
     CDS             1493..2038
                     /locus_tag="VW561_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9944162.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELKKK"
     gene            2050..2364
                     /locus_tag="VW561_001948"
     CDS             2050..2364
                     /locus_tag="VW561_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEU9944163.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKNHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            2381..3073
                     /locus_tag="VW561_001949"
     CDS             2381..3073
                     /locus_tag="VW561_001949"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEU9944164.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNAQEQVVFGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQKSLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            3150..3641
                     /gene="rocS"
                     /locus_tag="VW561_001950"
     CDS             3150..3641
                     /gene="rocS"
                     /locus_tag="VW561_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEU9944165.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            3739..4329
                     /locus_tag="VW561_001951"
     CDS             3739..4329
                     /locus_tag="VW561_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEU9944166.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            4338..6101
                     /locus_tag="VW561_001952"
     CDS             4338..6101
                     /locus_tag="VW561_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEU9944167.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDRYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAMLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         1928 a         1057 c         1373 g         1904 t
ORIGIN      
        1 aatgggttaa ggaagagact ttagagcatt ttttcattca agagtgcgga atgatttgaa
       61 atatggtata ataaaaggga atttctacag aaaagagaag attatgtcaa attttgccat
      121 tattttagca gcgggtaaag ggactcgcat gaaatctgat ttgccaaaag ttttgcacaa
      181 ggttgcgggt atttctatgt tggaacatgt tttccgtagt gtgggagcta tccaacctga
      241 aaagacagta acagttgtag gacacaaggc agaattggtt gaggaggtct tggctggaca
      301 gacagaattt gtgactcaat ctgaacagtt gggaactggt catgcagtta tgatgacaga
      361 gcctatctta gaaggtttgt caggacacac cttggtcatt gcaggagata ctcctttaat
      421 cactggtgaa agcttgaaaa acttgattga tttccatatc aatcataaaa atgtggccac
      481 tatcttgact gctgaaacgg ataatccttt tggctatgga cgaattgttc gtaatgacaa
      541 tgctgaggtt cttcgtattg ttgagcagaa ggatgctaca gattttgaaa agcaaatcaa
      601 ggaaatcaac actggaacat acgtctttga caacgagcgt ttgtttgagg ctttgaaaaa
      661 tatcaatacc aataacgctc aaggcgaata ctatattaca gacgtcattg gtattttccg
      721 tgaaactggt gaaaaagttg gcgcttatac tttgaaagat tttgatgaaa gtcttggggt
      781 aaatgaccgt gtggcgcttg cgacagctga gtcagttatg cgtcgtcgca tcaatcataa
      841 acacatggtc aacggtgtta gctttgtcaa tccagaagca acttatatcg atattgatgt
      901 tgagattgct ccggaagttc aaatcgaagc caatgttacc ttgaaagggc aaacgaaaat
      961 tggtgctgag actgttttga caaacggtac ttatgtagtg gacagcacta tcggagcagg
     1021 agcggtcatt accaattcta tgattgagga aagtagtgtt gcagacggtg tgacagtcgg
     1081 tccttatgct cacattcgtc caaattcaag tctgggtgcc caagttcata ttggtaactt
     1141 tgttgaggtg aaaggatctt caatcggtga gaataccaag gctggtcatt tgacttatat
     1201 cggaaactgt gaagtgggaa gcaacgttaa tttcggtgct ggaactatta cagtcaatta
     1261 tgacggcaaa aacaaataca agacagtcat tggagacaat gtctttgttg gttcaaattc
     1321 aaccattatt gcaccagtag aacttggtga caattccctc gttggtgctg gttcaactat
     1381 tactaaagac gtgccagcag atgctattgc tattggtcgc ggtcgtcaga tcaataaaga
     1441 cgaatatgca acacgtcttc ctcatcatcc taagaaccag taggagccta tcatggagtt
     1501 tgaagaaaaa acgcttagcc gaaaagaaat ctatcaagga ccaatattta aactggtcca
     1561 agatcaggtt gaattaccag aaggcaaggg aactgcccaa cgggatttga ttttccacaa
     1621 tggggctgtc tgtgttttag cagtaacgga tgaacaaaaa cttatcttgg tcaagcagta
     1681 ccgcaaagct atcgaggctg tctcttacga aattccagcc ggaaaattgg aagtaggaga
     1741 aaacacagcc cctgtggcag ctgcccttcg tgaattagag gaagaaacag cctatacagg
     1801 gaaattagaa ctcttgtacg atttttattc agctattggc ttttgtaatg agaagttaaa
     1861 actatattta gcaagcgatt tgacaaaagt ggaaaatccg cgtccgcagg atgaggatga
     1921 aaccttggaa gtccttgaag tgagcttaga agaagcgaaa gaattaatcc aatcaggtca
     1981 tatttgtgat gccaagacaa ttatggctgt tcagtattgg gagttgaaga aaaaatagag
     2041 gaggtcagta tgggtaaatc tttattaacg gatgaaatga ttgaaagagc taatagaggc
     2101 gaaaaaattt caggtcctcc tttgctagat gataatgagg agactaagat tttaccaacc
     2161 tcttcttccc gttttggtta tgccaatcct aagaatcatg gttttagcca ggaaaccttg
     2221 aagattcagg tcgaaccatc tattcataaa agccgtcgca ttgaaaatac caagagaaat
     2281 gtcttcaatt ctaagttgaa taaaatctta ttcgcggtca tctttctctt gattttgctt
     2341 gttttagcaa tgaaactttt gtaatagaaa aggaattgaa atgaaaatag gaattattgc
     2401 tgctatgcca gaagaactgg cttatctggt ccagcattta gataatgccc aggagcaagt
     2461 tgtttttggg aatacctatc atacaggaac cattgcttct catgaagtcg ttcttgtaga
     2521 aagtggaatt ggtaaggtca tgtctgctat gagtgtggcg attttggctg atcatttcca
     2581 ggtggatgcc cttattaata cgggttcagc tggggcagta gcagaaggta tcgctgttgg
     2641 ggatgtcgtg attgctgaca aattagccta tcatgacgtg gatgtcacag cttttggcta
     2701 tgcttatgga caaatggcgc aacaaccgct ttatttcgaa tcagacaaaa cctttgttgc
     2761 tcaaatccaa aagagtttat ctcaattgga ccaaaactgg catcttggtt tgattgctac
     2821 aggagatagt tttgttgcag gaaatgacaa gatagaagcg attaagtccc atttcccaga
     2881 agttttagcc gtggagatgg agggggcagc tattgctcaa gcagcgcatg ccctcaatct
     2941 cccagtctta gtcatccgag ctatgagtga caatgccaac catgaagcaa acatcttttt
     3001 tgatgagttt attatcgaag ctggacgtcg ctctgcccaa gtcttgttga cctttttgaa
     3061 ggctttagat taagcggaaa tttgacagtt tttctagctt atgataagat ttaagtaaag
     3121 aaaagctaga aaacgtttca gaggatatta tgagtattga aatgaccgtc agtgagattg
     3181 cagaggtctt aggattatct cgccaagcaa tcaataaccg tgtcaaagaa ttaccagaag
     3241 aagacacaga taaaaatgac aaaggggtaa cagttgttac cagaagtggc ttgattaagc
     3301 tagaagaaat ctataaaaaa acgatttttg aagatgaacc tgtcagtgaa gatgtcaaac
     3361 aacgtgaact gatggagatt ctagtggatg agaagaatgc agaaattctt cgtctttatg
     3421 aacaattaaa agccaaggat cgtcagttat cagaaaaaga cgagcagatg cgtatcaaag
     3481 accgtcagat tgctgagaaa gacaaacaat tagatcagca acaacaattg acccttcaag
     3541 ccatgaagga tcaagaaaat cttaagctag agctggacca agcaaaagaa gaagtccaat
     3601 ccactaaaaa aggctttttt gctcgtttat ttggaggata atcaaggagc tgtttaggtt
     3661 aaatgctaac ctgatggctt cttttgtttg tgaatagtat agttgattag aacagagaca
     3721 gtagaggaga ggtacagaat ggaacgatta gaagattaca ttgcttttga tttagaattt
     3781 aatcagcacg aaggacaaac acacttgatt caagtatcgg cagtgcgttt tagagatggt
     3841 cgggaaatcg atgcctatga ttcctatgtt cataccagtg tccctctaaa aagctttatc
     3901 aatggtttga ccgggattac agctgagacc ttaaaagacg ctcctccagt tgagaaagtc
     3961 ttagaagaat tccaagagtt tgtgggttct ttgctgatga taggctacaa tgctgctaaa
     4021 agtgatttac ctattttagc agagcatggt ttagactata gccaacagta caaggtggat
     4081 ttgtatgatg aagcttttga acgtcggagt tcagatttac atggtattgc caatctcaaa
     4141 ttgcaaactg ttgcctcatt tcttggattt caaggtcagt ctcataatag tttagaggat
     4201 gctcggatga cggcgcgtgt ttacgaatcc ttcttagagt cggatcaagc tagggaattg
     4261 ttagagacag aaagtagctt atcaaacaat ccttttggtg gcttggactt gtctcaatta
     4321 tttgactagg agtgcctatg aaacctgaaa aacctaaaaa attgggtctt aaacagattt
     4381 acctcaatct tgataaaatc ctctttctat ttttcttgat ttttatgata gtggagtatt
     4441 tgtgggtacc attcaattca tggttggcta gcttcttatt agatcagaca ggttatcttt
     4501 ttatttccta taacaatgtc ttagccatcc ttactcattc tccattgatt agtctagctt
     4561 ttttagcctt gattgtaatt aaccttctgg ttgcttattt tcaaatagga ctcctcttta
     4621 ttggtgctcg tcatctcctc tataatgaaa agcgaaggct aattgagtat atccgcaagg
     4681 ttttccatga aagttttgga tttatgaaaa agctaacttg cgctaaagcc ttgtttatct
     4741 ttttctatat agctatgctg tttcctttta tacggaaaat gctgaagatt tattatctta
     4801 ataaaatcgt cattccagag tttatccaag cttatttaga agatagatat tggatgtggt
     4861 ggctgagcat cctacttcta tctcttatct ttctctatgt ctctgtcaga ttgatgtttg
     4921 cccttccgaa gattgtatat gatcagctga ctgttcgaga agcagtgatg tttagcttgg
     4981 agaaaaccaa gaagagagtt actttttatg cttggaattt attttacatc ttgctcaaag
     5041 cacatttatt attttatctt cctttgattc ctttattatt ggctcaaact ttggtagatg
     5101 atatcactca gagagagtct ctgattcttg gtattttgaa ttttgtcgtg attaaaaatc
     5161 tctattatat ggctctgact tattttttgg ttaaatttgt ttcatttttg acaggaaagg
     5221 agctggatat gcttcctagg agagaaaaag atcacatcgt gcgatggggt gtcatgactt
     5281 gtgccagtct tttctttgcc ttagaaggtt atatttatct ggaggctccc atggttcatc
     5341 tacctcaact tatttctcac cgaggggttt ccaatgcaaa tgggattcaa aatacagtag
     5401 agtccttgga aactacagca caactcaaac cagacttggt ggagacggac gtgcaggaaa
     5461 caaaagatgg gcagtttgtc atgatgcatg atgctaactt gaaaaatcta gcaggtatca
     5521 ataaaagtcc tcaagactta aacttggagg agcttaaagg gattgatatt tttgaaaatg
     5581 gctaccagac taaaatttca agctttgaag attatctcag tcgagccaac gaacttggtc
     5641 aaaaattact aattgaaatt aaaaccagta aaaaagatag tccagacatg atgaaccgct
     5701 ttttagcccg ttatgctgca atgctcaaga tttatggaca tcaaatccag tctttagact
     5761 accatgttgt cgaaaaagta agacagtatg atgccgaact gccagtttat ttcatcatgc
     5821 cctacaattc tgtctttcct aaaacaagag cgacaggata tactatggag tactcaacct
     5881 tggatgaata ttttgtaagc aaactatgga caacggatca gaaactttat gtctggactg
     5941 tcaatgattc agaagctatc agcaaatctc ttcacttagg agtagatgga gtgattacag
     6001 atgatttgga aaaagttcag cgggaaatag aagtagccca agaagatcca gaatatacgg
     6061 atttgctctt gaaaaaagca ttggaattct ttgaatttta gtaataaaaa agaggaacgg
     6121 ttgttcctcc catgataggt aagtattctt gtgaatgctt gcttttttgt ggttgcaatc
     6181 cgtttacgag aattgtttat attgttcttg tttcaattta ctttatagtg ctttgaagtt
     6241 ggaatgctac actataactt at
//
LOCUS       DAVKQL010000086         6123 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVKQL010000086 DAVKQL010000000
VERSION     DAVKQL010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6123
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.086"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            217..501
                     /locus_tag="VW561_001953"
                     /pseudo
     CDS             217..501
                     /locus_tag="VW561_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
     gene            710..1369
                     /locus_tag="VW561_001954"
     CDS             710..1369
                     /locus_tag="VW561_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEU9944168.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            1394..2461
                     /locus_tag="VW561_001955"
     CDS             1394..2461
                     /locus_tag="VW561_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9944169.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            2565..3575
                     /locus_tag="VW561_001956"
     CDS             2565..3575
                     /locus_tag="VW561_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9944170.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARVLVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            3587..5278
                     /locus_tag="VW561_001957"
     CDS             3587..5278
                     /locus_tag="VW561_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9944171.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            5281..5991
                     /locus_tag="VW561_001958"
     CDS             5281..5991
                     /locus_tag="VW561_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU9944172.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         2054 a          842 c         1171 g         2056 t
ORIGIN      
        1 ggacagtcaa atcgatttct aacaatgttt tagaagtaga ggtgtactat tctagtttca
       61 atatattata ttatcgctta ccaatatttg ctaaactttc agaaaaaaag ttggagtata
      121 tgatgctagg tgactgcgtc atgttagtaa acgaaatgga aataactgat catagagtag
      181 ataacttatt tgaaaaaggg aaaaatgaga taaaagattc atgtagtaga tttagcaata
      241 ggacatataa aagctttaga aaaagtatct gaaaaaacag atgtttatat ctataaccta
      301 ggctcgggag aaggcactag tgtattacaa cttgtaaata catttgaaag tgttaataag
      361 atccctattc cttataaaat agttccaaga cgttcaggag acgttgcaac ttgttatgca
      421 aatgcagaca aagcatataa ggaattaaat tggaggacaa caaaatcgat tgaagacatg
      481 tgtagagata catggaatta gcaatcaaaa aatcccaatg gctataattg atgatactgt
      541 ctatatgagt gctgtatttt ggaaaaatta cttaaccttt taatgaaatg catatttaat
      601 gaaagataaa ttaaaaaaat gtttgacagc gcttgacaaa ggaaacggtt tcattgtata
      661 atgtaaacat aatgacaccg atgtcaaata taaaaaagaa ggtctttata tgaaaaactt
      721 aacaaaaatt aagttcaaag agaatgggga atttaatcat ttccctggga atacagttgt
      781 agcaaatctt tatactaaac aagatttgat ggaagttgtt gatattattc aatcacgtta
      841 tagagaatta ccatttatcg ataagtttac tttaactcca aggaattcta ttcacatgac
      901 tgtaattgaa ttgttgtgcc atgaaaatcg tgaaacggag ttttggagca gtaatcttcc
      961 tctagataca cctttacagg aaatacatga ttactttgct aaacaacttg aaatttttcc
     1021 attgttggat gaagaaattc atatgcgtat aactgaaatg ggaaaacaaa acatactagt
     1081 tgaacctgca gatgaagctt ctgcaaagag attagaagaa attcgtactt atgtctcaga
     1141 aaaagcaggt gtttgtttcc ctaatcatga tagatatcaa ttccatattt caattgggta
     1201 tcttcggatt cctctaaccg aagaggagga agaagagttt actaaagtca gagcagaatt
     1261 aactgaaatt ttattagaga agattccaac tattactgta aaccgtattg actatactgt
     1321 atttgaagat atgagacaat ttgttccata tcacgaaaaa tttaaataaa ataataaaaa
     1381 aggagaactt atcatgaaaa aaatgaaagt ttggtctact gtacttgcaa cgggagttgc
     1441 tcttactaca cttgctgctt gctctggagg ttcaaattct acgactgctt cttcatctga
     1501 agaaaaagct gataaaagtc aagaattagt tatctattcg aactcagtct caaatggtcg
     1561 tggtgattgg ttaactgcta aagcaaaaga agctggtttt aatataaaaa tggttgatat
     1621 cgctggcgct caattagcag accgtgttat tgctgagaag aataatgcag ttgcagatat
     1681 ggtatttgga attggtgctg ttgactcaaa taaaattaga gatcaaaaat tactagtaca
     1741 gtacaagcct aaatggttag ataaaattga tcaatcttta tcagataaag ataattatta
     1801 taatcctgtg attgttcaac cattagtttt aattggggcg cctgatgtaa aagaaatgcc
     1861 taaagattgg actgaattag gtagtaagta taaaggtaaa tattcaattt atggtcttca
     1921 aggtggtaca ggacgggcaa ttctagcaag tatcttagtt cgttaccttg atgaaaaagg
     1981 agatttaggc gtatcagaaa aaggatggga agttgcaaaa gaatactttg caaatgccta
     2041 tactcttcaa aaaggtgaaa gctcagttgt taaagcgtta gataaagaaa gcccaattca
     2101 atacggtatg atctggggtt ctggagcgtt aattggacaa aaagaacaaa atgttgaatt
     2161 caaagttatg actcctgaga ttggtgtacc atttgtaact gaacaaacta tggttttaag
     2221 cactagtaaa aaacaagcgt tagctaaaga atttattgat tggtttggtc aatcagaaat
     2281 tcaagtagaa tatagtaaaa aatttggatc tattcctgca aataaagatg ccctaaaaga
     2341 gttacctgaa gatacgaaaa aatttgttga tcaagtgaaa ccacaaaata ttgactggga
     2401 agctgttgga aagcatttgg atgaatgggt agaaaaagct gaattagaat acgtaaaata
     2461 agaaataaga gttatttttt atgtccaagt ctctctatta tttctaaatt taaggaagaa
     2521 ataaagagag gcttttggtt ttagtaatag agggagttag tgagatgatt aaatttgata
     2581 atattcaaat taaatatggt gattttgttg caattgataa tctgaattta gatatacatg
     2641 aaggggaatt ctttacattt cttgggcctt caggatgtgg taaatcaact actttgagag
     2701 cattggtagg ttttttagat ccatcatcag gaagtattga agttaatgga acagatgtca
     2761 ctcatttgga acctgaaaag cgtgggattg gtattgtatt tcaatcttat gcgctatttc
     2821 caactatgac tgtttttgat aatattgcat ttggtttaaa agttaagaag gtagctccag
     2881 atgttattaa agctaaagta tcagcagtgg cagcaaaaat taagatctct gatcaacagt
     2941 tacagcgtaa tgtatcagaa ttatctgggg gtcaacaaca aagggtagca ttggctcgtg
     3001 ttctggttct tgaacctaaa attctttgtc tagatgaacc attgtcaaac cttgacgcaa
     3061 aattacgtgt agatttgaga aaagagttga aaagacttca aaaagagtta ggtattacta
     3121 ctttatatgt tactcatgat caagaggaag ccttgacttt atctgataga attgcagtct
     3181 ttaacaatgg atacatcgaa caggtcggta caccagtaga gatttatcat aattctcaaa
     3241 ctgaatttgt atgtgatttt attggagata ttaatgtttt gaccgatgaa acagtccacg
     3301 aagtattatt gaaaaataca agcgttttct tagaggataa aaaaggatac attcgattag
     3361 agaaagttcg attcaatcgt gaaactgaac aagattttat tctaaaaggg acaattattg
     3421 atgttgagtt ttctggagtt acaattcact atacaataaa agtttctgaa agtcagattc
     3481 ttaatgtaac aagtattgat agtcaggctg ctattagatc tgtcggagaa agtgtggaat
     3541 tatttatcac accatcagac gttctgcaat tttaaggagg tgcagtatgc gtcataaatt
     3601 aaatttaaaa gattggctta ttcgtttagg gttaatctgg ttcttagtaa catttattat
     3661 ttatccaaac tttgatctag tagtgaatgt atttgtaaaa ggaggagaat tttcccttga
     3721 tgctgtacat cgtgttctaa aatctcagag ggcacttcag agtattatga acagttttaa
     3781 gttagcattt tcactcatta ttacagttaa tgtcgtaggt attctttgtg ttctatttac
     3841 agagtacttt gatattaaag gtgctaaaat tttaaaatta ggttatatga cctctttaat
     3901 ttatggagga gtggttttag cgactggata taaatttgtc tatggtcctt atggattgat
     3961 tacaaaattt ttacaaaatg ttatcccttc tttagactct aactggttta ttgggtatgg
     4021 tgcagtctta ttcattatga cattttcagg aactgctaat catacattgt ttttaacaaa
     4081 tacaattcga agcgttgact atcacactat tgaggctgct cgaaatatgg gagcaaaacc
     4141 atttactgtt ttccgaaaag tagtgttacc aaccttaatt ccaactctat ttgcacttac
     4201 tattatggtt tttcttagtg gtttatctgc agtagcagca cccatgattg ttggtggtaa
     4261 agaatttcaa actataaatc caatgattat tacatttgca gggatgggga attctcgtga
     4321 tttagctgcc ttacttgcaa ttattttagg tattgcaact acaattttgc ttactatcat
     4381 gaataagata gaaaaaggtg gaaattatat ttctatctct aagactaaag cgcctcttaa
     4441 aaaacaaaaa attgcgtcta agccttggaa tatcattgct cacattgtag catatggatt
     4501 gttcacagtt ttcatgcttc cactaatttt tatagtatta tactcattta cagatccagt
     4561 tgcaattcaa acaggtaact taacattatc aaactttact ttagaaaatt atcgcttatt
     4621 ctttagtaat agtgcggcat tctctccatt cttggtcagc tttatttatt ctattattgc
     4681 tgcgacaaca gcaacaattc tcgcagttgt atttgctcgt gttgtcagaa aacataaatc
     4741 tcgttttgat ttcttatttg aatatggtgc tctacttcct tggttactac caagtacact
     4801 tttagcagta agtttattat ttacttttaa tcagccacaa tttcttgtct tgaatcagat
     4861 tttggtaggt agtttggtaa ttctacttat tgcatatata gttgtaaaaa tcccattttc
     4921 ttatagaatg gtacgtgcta ttttatttag tgttgatgat gagatggaag atgcagcaag
     4981 aagtatgggt gcttcacctt tttatactat gatgaaggtt atcattccat ttattttacc
     5041 ggttgttctc tctgttattg ctttaaactt taactcttta ttaactgact tcgacttatc
     5101 tgtattcctt taccatcccc tagctcaacc attaggtatt acgattcgat ctgcaggtga
     5161 tgaaacagca acatctaatg cacaagctct ggtatttgtt tatacaattg ttctgatgat
     5221 tatttctgga acggtattat acttcacaca aagaccgggg cgtaaagtaa ggaaataatc
     5281 atgacagcca ctagtcttgg gttatcaaat attgaaatag ttgtcaggat tgttttatca
     5341 gtagtcattg gtagtataat tggtttagag agagggagca aatcccagcc tgcaggcatc
     5401 cgaacttata gtattgtttg tctagctgca tgtttgatta tgatgacgaa tgaatacgta
     5461 tcttataaat ttgggacagg agatcctaca cgattaggag ctcaagttat atcaggtgtg
     5521 ggttttctag gcgctggaac gattcttatt acagataaaa agaaaattac aggtctgaca
     5581 actgcagcag gcatttgggc ttcggcagga attggattag ctattggagt aggtttttat
     5641 gagggagctc ttttagtagc catttctgtt tggggtgtga tatccatgtt ccaaccacta
     5701 aaaaaatatc tgcaaaatcg ttctaaaatg attgaattgt atatagtagt taaatctaca
     5761 gaagcctata atcgtgtatt ggtttattgt gcagaaaatg gtatcagaat gaccgattca
     5821 agaacagcgt ttggagatgt taattctgac agaattgaat actttgatgt tccggacaaa
     5881 aaaatagcgt catttattac tctggaatta tcaggtagat ttgaacatct tcgactgatg
     5941 gaagaaattg ctaatattgt aggtgttatt tatgtcgaag aaattagctg aaatttagta
     6001 aacaaaggga cagatgtttt tgtccttttg ttttttagat gttttaatca tctaattttt
     6061 gagtagattt ttgctcagag gtttttatga aagattataa aatcaatttt gatttaggta
     6121 aaa
//
LOCUS       DAVKQL010000087         5910 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVKQL010000087 DAVKQL010000000
VERSION     DAVKQL010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5910
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.087"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            1..401
                     /locus_tag="VW561_001959"
                     /pseudo
     CDS             1..401
                     /locus_tag="VW561_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            556..756
                     /locus_tag="VW561_001960"
     CDS             556..756
                     /locus_tag="VW561_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944173.1"
                     /translation="MVVSASASSTSPQAQEQVDKSELRALSQELDQHLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            781..1197
                     /locus_tag="VW561_001961"
     CDS             781..1197
                     /locus_tag="VW561_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944174.1"
                     /translation="MKAALDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETN
                     SVPAGVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKA
                     QNLLQETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1428..2135
                     /gene="ccdA2"
                     /locus_tag="VW561_001962"
     CDS             1428..2135
                     /gene="ccdA2"
                     /locus_tag="VW561_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU9944175.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYGMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            2146..2703
                     /gene="sdbB"
                     /locus_tag="VW561_001963"
     CDS             2146..2703
                     /gene="sdbB"
                     /locus_tag="VW561_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEU9944176.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            3078..4469
                     /locus_tag="VW561_001964"
     CDS             3078..4469
                     /locus_tag="VW561_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU9944177.1"
                     /translation="MNIFRTKNVSLYKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIALPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKVPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4612..5529
                     /gene="adcAII"
                     /locus_tag="VW561_001965"
     CDS             4612..5529
                     /gene="adcAII"
                     /locus_tag="VW561_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEU9944178.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDSDVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLDRVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTENNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            5537..>5910
                     /locus_tag="VW561_001966"
     CDS             5537..>5910
                     /locus_tag="VW561_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU9944179.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVAYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGG"
BASE COUNT         1813 a         1048 c         1245 g         1804 t
ORIGIN      
        1 ctgtcatcca actaccatcc actatgcgct caaagctatg ggctacactc gaaaaaaaga
       61 accacaccta ctatgaacaa gagccagaaa aagtagcctt atttcttaag aattttaata
      121 gtttaaagca cctagcacct gtttagattg acgaaacagg attcgatact tatttttatc
      181 gagaatatgg tcgctcatta aaaggtcagt taagaagagg caaagtatct ggaagaagat
      241 atcagaggat ttctttggtt gcaggtctaa caaatggtga gttaatcgct ccaatgactt
      301 acgaagagat gatgacgagc gacttttttg aagcttggtt tcagaagttt ctcttaccaa
      361 cattaaccac accatcggtt attatagtaa aacgaaataa gaataggaca aatcgatcag
      421 gacagtaaaa tcgaattcta acaatgtttt agaagtccat atgtactatt ctagtttcaa
      481 tataccataa ctgctcctag tcaacctgca agtcctgtcc cagctccaag ctcagctccg
      541 actcaagatc ctagagtggt agtaagcgct tcggctagct caacgagccc acaagctcaa
      601 gagcaagttg acaagtctga acttcgtgcc ttgagtcaag agttagacca acacttgaaa
      661 gctttggcaa cagtatctga tccgaaaatc gatgcaacca aggctgtcct cctagatgct
      721 caaaaagctc cggaagatag tgccttgaca gagtaaggtc tgcatactgc agtagagtct
      781 gtgaaggctg ccttggactc tctcaaagat gtgaaagcaa atgctagcga cagcaagcct
      841 gcacaggaca agaaggatgc aaaacaagga acggaagata gtaaggattc agataagatg
      901 actgaaacaa actcagttcc ggcaggagtg attgtggtca gtctacttgc cctcctaggc
      961 gtgattgcct tctggctgat tcgccgtaag aaagagtcag aaatccagca attaagcacg
     1021 gaattaatca aggttctagg acagctagat gcagaaaaag cggataaaaa agtccttgcc
     1081 aaagcccaaa accttctcca agaaaccctt gatttcgtga aagaagaaaa tggctcagca
     1141 gagacagaaa ctaaactagt agaggagctt aaagcaatcc ttgacaaact caagtaaaac
     1201 atgatttatg aaatgtcaga cttaaaagac attttcgttc acaaaagcag cgacgagtaa
     1261 tccttgtcgt tgctttttgg ctattttggg ggataagatc cgccttgact agcaaaagaa
     1321 aagattggtc caatagtgcc cgatacgatt ttgcgtagca gaaagcttac aaataaaatt
     1381 gaatatagta aaataggatt agaattatta agaaagttgg ttctttattg gaaacgatag
     1441 tatttttaat ctctgttttt ctagcaggtg ttttatcctt tttttctcct tgtatttttc
     1501 ctcttctgcc agtctatgct gggattttat tggatgatca agaaagtgca aaaagctttt
     1561 ctttgtttgg gagaaaggtt ctctggtcag gcttgattcg aacactttgc tttatcgctg
     1621 gtatctctct cattttcttt attctaggct ttggtgctgg ttactttggt catattctct
     1681 atgcaaattg gtttcgatat ggcatgggag ctattattat cattttgggt cttcatcaga
     1741 tggaaatttt tcatttgaag aaattagaag ttcaaaaaag ttttaccttt aaaaaatcag
     1801 attctaatcg ttattggtca gcttttttac ttggtattac ctttagcttt ggttggacac
     1861 cttgtattgg tccagtttta agttctgttt tagcacttgc ggcttctgga ggcaatggcg
     1921 cttggcaagg agcgatttat actctcattt acactctggg catggccctt cctttcttgg
     1981 tattggcact agcttcaggt ctagtcatgc catattttag taaaatcaag cgtcatatga
     2041 tgctactaaa gaaaattggt ggtttcctca ttgttttaat gggaattttg ttactattag
     2101 gacaagtaaa tgttctagct ggaatttttg aataaaggag gacaaatgaa aaaagtaatg
     2161 tttgctggct taagtctctt gtcattagtt gtattgatgg cctgtggtga ggaagaaact
     2221 aaaaagactc aagcagcaca acagccaaaa caacaaacga ctgtacaaca aattgctgtt
     2281 ggaaaagatg ctccagactt cacattgcaa tccatggatg gcaaagaagt taagttatct
     2341 gattttaagg gtaaaaaggt ttacttgaag ttttgggctt catggtgtgg tccatgcaag
     2401 aaaagtatgc cagagttgat ggaactagcg gcgaaaccag atcgtgattt cgaaattctt
     2461 actgtcattg caccaggaat tcaaggtgaa aaaactgttg agcaattccc acaatggttc
     2521 caggaacaag gatataagga tatcccagtt ctttatgata ccaaagcaac caccttccaa
     2581 gcttatcaaa ttcgaagcat tcctacagaa tatttaattg atagccaagg aaagattgga
     2641 aagattcaat ttggtgctat cagtaatgcg gatgcagaag cagcatttaa agaaatgaac
     2701 tagaatcgat aaaatctgat tacaagatgt agtcagattt tggtaacttc taaaactaac
     2761 ttctagtttc ccacaaccta gcttttagta tgagaaaaac gtgtgacacc agtggaaatc
     2821 cgtcttagac taacttccac tggttttctt tttcttgata tataagggtt caaaagcgaa
     2881 aagactcaga aaaaagtgca cgacaaatag ccctaaaaca gagtcgtttt agagtaactt
     2941 ccagttttgg gagagagatg gaagttactt tgagaagtta cggtagtcag atttttttag
     3001 aaaaacattt tataaatatt cacatatctc ctatatttat ggtaaaatag aatcatcagt
     3061 ttattttgga gtcaaagatg aatatattta gaacaaagaa tgttagttta tataaaacag
     3121 agatgcatag gcatttgaag ttatgggatt tgattttgct gggtattgga gccatggtag
     3181 ggacaggtgt ctttacaatc acaggtactg cagctgctac acttgctggt ccagcactag
     3241 tgatttcaat cgttatttct gccttgtgtg tgggattatc agccctcttt tttgcagaat
     3301 ttgcctcgcg agtacccgct acaggaggtg cctatagtta cctctatgct atcttaggag
     3361 aattccctgc ctggttggct ggttggttaa ccatgatgga gttcatgaca gccatatcag
     3421 gcgtagcttc gggttgggca gcttatttta aaggcttgct ctctcaatac gggatagccc
     3481 ttcctcaggc tttaaatggt acctttaatc ctcaagcagg gacatttgtt gatttattgc
     3541 ctattcttgt cttggtcttg gtgacttcgc ttgttttact aaatgccaag gcagccttac
     3601 gctttaattc gattctagtt attttgaaat tttccgcttt agctctcttt gttttagtag
     3661 gaatttggaa tattaagttt gataattgga gcaattttgc tccttacggt tttggacaaa
     3721 tctatggagc tagtactggt attatggctg gtgcgtcctt gatgttcttc ggttttttgg
     3781 gatttgaatc catctctatg gctgtagatg aagtcaagac tcctcaaaaa aatattccta
     3841 gagggattgt cttatcgctt tctatcgtaa ccattctcta tgccttggtg acccttgttt
     3901 tgactggtgt ggttcactat agtcatttaa atgtcgatga tgccgttgcc tttgcccttc
     3961 gtagtgttgg gattagttgg gcagccaact atgtgtcatt agtggctatc ttgaccttga
     4021 ttacagtttg tatttcgatg acctatgccc tatcgcgtat gatttacagt ttagcacgtg
     4081 acggcttagt gcctgctgcc tttaaggaac tgacgaagac tagcaaggta ccaaagaatg
     4141 ctactatctt gacaggtcta gcttcagcag tagcagcagg aatgttccca ctagccagta
     4201 ttgcagcctt tttaaatatt tgtaccttag cctacttgat catgctggct tatggtctga
     4261 ttcgcttacg gaaagaaaaa ggaatgccca aagctgggga atttaaaaca ccattggtac
     4321 ctctgttacc aattttatcg atcatcattt gtttatcctt catgttacag tataatatga
     4381 acacctggat tgctttccta gttgcgttgt tagtaggaag tattatttac ttcacttatg
     4441 gctataagca ttctaccata gaagaataaa gtgaaagtct attgagttgt tgacactcag
     4501 tagattttta tatgtgaaag aaatttcaaa ttttttctaa aaatatcttg acagattaaa
     4561 ttttcaggag tagaatattt actagttaat taaatggtta aggagttgtt catgaagaaa
     4621 caaaatttat ttttagtcct gttaagtgtc tttcttttgt gcttgggggc ttgtggtcaa
     4681 aaggaaagtc agacaggaaa ggggatgaaa attgtgacca gtttttatcc tatctacgct
     4741 atggttaagg aagtatctgg tgacttgaat gatgttcgga tgattcagtc aagtagtggt
     4801 attcactcct ttgaaccttc ggcaaatgat atcgcagcca tctatgattc agatgtcttt
     4861 gtttaccatt ctcatacact cgaatcttgg gcaggaagtc tggatccaaa tctaaaaaaa
     4921 tctaaagtga aggttttaga ggcttcagag ggaatgactt tagaccgcgt ccctggacta
     4981 gaggatgtgg aagcagggga tggagttgat gaaaaaacgc tctatgaccc tcacacatgg
     5041 ctagatcctg aaaaagctgg agaagaagcc caaattatcg ctgataaact ttcagaggtg
     5101 gatagtgagc ataaagagac ttatcaaaaa aatgcgcaag cctttatcaa aaaagctcag
     5161 gaattgacta agaaattcca accaaaattt gaaaaagcga ctcagaaaac atttgtaaca
     5221 caacatacag ccttttctta tctagcgaag agatttgggc ttaatcaact tggtattgca
     5281 ggtatctctc ctgaacaaga accaagtcca cgacaactaa cagaaattca ggaatttgtt
     5341 aagacctata aggttaaaac gatttttaca gaaaataacg cttcttcaaa agtagctgaa
     5401 actcttgtca aatcaacagg tgtgggtctt aaaactctga atcctttaga gtcagaccca
     5461 caaaatgaca agacctattt agaaaatctt gaagaaaata tgagtattct agcagaagaa
     5521 ttaaagtgag gaaagaatga aaatcaataa aaaatatcta gcaggttcag tggcagtcct
     5581 tgccctaagt gtttgttcct atgaacttgg tcgtcaccaa gctggtcagg ttaagaaaga
     5641 gtctaatcga gttgcttata tagatggtga tcaggctggt caaaaggcag aaaatttgac
     5701 accagatgaa gtcagtaaga gagaggggat caacgccgaa caaattgtta tcaagattac
     5761 ggatcaaggt tatgtgacct ctcatggaga ccattatcat tactataatg gcaaggttcc
     5821 ttatgatgcc atcatcagtg aagaacttct catgaaagat ccgaattatc agttgaagga
     5881 ttcagacatt gtcaatgaaa tcaagggtgg
//
LOCUS       DAVKQL010000088         5869 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVKQL010000088 DAVKQL010000000
VERSION     DAVKQL010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5869
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.088"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            105..584
                     /locus_tag="VW561_001967"
     CDS             105..584
                     /locus_tag="VW561_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU9944180.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
     gene            678..749
                     /locus_tag="VW561_001968"
     tRNA            678..749
                     /locus_tag="VW561_001968"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:711..713,aa:Glu,seq:ttc)
     gene            1002..2547
                     /locus_tag="VW561_001969"
     rRNA            1002..2547
                     /locus_tag="VW561_001969"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            2599..2671
                     /locus_tag="VW561_001970"
     tRNA            2599..2671
                     /locus_tag="VW561_001970"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2632..2634,aa:Ala,seq:tgc)
     gene            2796..5697
                     /locus_tag="VW561_001971"
     rRNA            2796..5697
                     /locus_tag="VW561_001971"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            5774..>5869
                     /gene="rrf"
                     /locus_tag="VW561_001972"
     rRNA            5774..>5869
                     /gene="rrf"
                     /locus_tag="VW561_001972"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1725 a         1140 c         1677 g         1327 t
ORIGIN      
        1 gcctctcttt tttgtgcagt ttaggagcta aagggaacag aatggagaaa atggaacaaa
       61 tgtgttttct aatctgttag actgtatcta gaaaggggaa aattatgatt aaagaattgt
      121 atgaagaagt ccaagggact gtgtataagt gtagaaatga atattacctt catttatggg
      181 aattgtcgga ttgggaccaa gaaggcatgc tctgcttaca tgaattgatt agtagagaag
      241 aaggactggt agacgatatt ccacgtttaa ggaaatattt caaaaccaag tttcgaaatc
      301 gaattttaga ctatatccgt aagcaggaaa gtcagaagcg tagatacgat aaagaaccct
      361 atgaagaagt gggtgagatc agtcatcgta taagtgaggg gggtctctgg ctagatgatt
      421 attatctctt tcatgaaaca ctaagagatt atagaaacaa acaaagtaaa gagaaacaag
      481 aagaactaga acgcgtctta agcaatgaac gatttcgagg gcgtcaaaga gtattaagag
      541 acttacgcat tgtgtttaag gagtttacta tccgtaccca ctaggaagtc atgcaaaaaa
      601 aatgaaaaaa attagaaaaa gtagttgaca aagtttgaaa agactgtata atagtaagag
      661 ttgaaaataa caactcaggt ccgttggtca aggggttaag acaccgcctt ttcacggcgg
      721 taacacgggt tcgaatcccg tacggactat ggtatgttgc ggttggaaca cttgatgaaa
      781 aaaagtttaa aaaagcttaa aaatcttcaa aaaaagtgtt gacaagcgaa agcggctgtg
      841 atatactaat atagttgtcg cttgagagaa gcaagtgaca aagacctttg aaaactgaac
      901 aagacgaacc aatgtgcagg gcgctacaac ataagttgta gtactgaaca atgaaaaaac
      961 aataaatctg tcagtgacag aaatgagtaa gaactcaaac ttttaatgag agtttgatcc
     1021 tggctcagga cgaacgctgg cggcgtgcct aatacatgca agtagaacgc tgaaggagga
     1081 gcttgcttct ctggatgagt tgcgaacggg tgagtaacgc gtaggtaacc tgcctggtag
     1141 cgggggataa ctattggaaa cgatagctaa taccgcataa gagtggatgt tgcatgacat
     1201 ttgcttaaaa ggtgcacttg catcactacc agatggacct gcgttgtatt agctagttgg
     1261 tggggtaacg gctcaccaag gcgacgatac atagccgacc tgagagggtg atcggccaca
     1321 ctgggactga gacacggccc agactcctac gggaggcagc agtagggaat cttcggcaat
     1381 ggacggaagt ctgaccgagc aacgccgcgt gagtgaagaa ggttttcgga tcgtaaagct
     1441 ctgttgtaag agaagaacga gtgtgagagt ggaaagttca cactgtgacg gtatcttacc
     1501 agaaagggac ggctaactac gtgccagcag ccgcggtaat acgtaggtcc cgagcgttgt
     1561 ccggatttat tgggcgtaaa gcgagcgcag gcggttagat aagtctgaag ttaaaggctg
     1621 tggcttaacc atagtaggct ttggaaactg tttaacttga gtgcaagagg ggagagtgga
     1681 attccatgtg tagcggtgaa atgcgtagat atatggagga acaccggtgg cgaaagcggc
     1741 tctctggctt gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag gattagatac
     1801 cctggtagtc cacgctgtaa acgatgagtg ctaggtgtta gaccctttcc ggggtttagt
     1861 gccgtagcta acgcattaag cactccgcct ggggagtacg accgcaaggt tgaaactcaa
     1921 aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga agcaacgcga
     1981 agaaccttac caggtcttga catccctctg accgctctag agatagagtt ttccttcggg
     2041 acagaggtga caggtggtgc atggttgtcg tcagctcgtg tcgtgagatg ttgggttaag
     2101 tcccgcaacg agcgcaaccc ctattgttag ttgccatcat ttagttgggc actctagcga
     2161 gactgccggt aataaaccgg aggaaggtgg ggatgacgtc aaatcatcat gccccttatg
     2221 acctgggcta cacacgtgct acaatggctg gtacaacgag tcgcaagccg gtgacggcaa
     2281 gctaatctct taaagccagt ctcagttcgg attgtaggct gcaactcgcc tacatgaagt
     2341 cggaatcgct agtaatcgcg gatcagcacg ccgcggtgaa tacgttcccg ggccttgtac
     2401 acaccgcccg tcacaccacg agagtttgta acacccgaag tcggtgaggt aaccgtaagg
     2461 agccagccgc ctaaggtggg atagatgatt ggggtgaagt cgtaacaagg tagccgtatc
     2521 ggaaggtgcg gctggatcac ctcctttcta aggataagga actgcgcatt ggtcttgttt
     2581 agtcttgaga ggtcttgtgg ggccttagct cagctgggag agcgcctgct ttgcacgcag
     2641 gaggtcagcg gttcgatccc gctaggctcc attggtgaga gatcaccaag taatgcacat
     2701 tgaaaattga atatctatat caaatagtaa caagaaaata aaccgaaaac gctgtagtat
     2761 taataagagt ttatgactga aaggtcagaa aataaggtta agttaataag ggcgcacggt
     2821 ggatgccttg gcactaggag ccgacgaagg acgtgacaaa cgacgatatg ccttgggtag
     2881 ctgtaagtaa gcgatgatcc agggatttcc gaatggggga acccaacagg tagtacctgt
     2941 tacccacatc tgttaaggat gtgaggagga agacgcagtg aactgaaaca tctaagtagc
     3001 tgcaggaaga gaaagcaaaa gcgattgcct tagtagcggc gagcgaaacg gcagaagggc
     3061 aaaccgaaga gtttactctt cggggttgta ggactgcaat gtggactcaa agattataga
     3121 agaatgattt gggaagatca gccaaagaga gtaatagcct cgtatttaaa atagtctttg
     3181 tacctagcag tatcctgagt acggcgggac acgtgaaatc ccgtcggaat ctgggaggac
     3241 catctcccaa ccctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
     3301 gtgaaaagca ccccgggagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
     3361 tcgagcccgt taatgggtga gagcgtgcct tttgtagaat gaaccggcga gttacgttat
     3421 gatgcgaggt taagttgaag agacggagcc gtagggaaac cgagtctgaa tagggcgcct
     3481 tagtatcatg acgtagaccc gaaaccatgt gacctaccca tgagcaggtt gaaggtgcgg
     3541 taagacgcac tggaggaccg aaccagggca cgttgaaaag tgcttggatg acttgtgggt
     3601 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
     3661 tagcgtcgac attagagatt cttggaggta gagcactgtt tgggtgaggg gtccatcccg
     3721 gattaccaat ctcagataaa ctccgaatgc caatgaatta tggtcggcag tcagactgcg
     3781 agtgctaaga tccgtagtcg aaagggaaac agcccagacc accagctaag gtcccaaaat
     3841 aattgttaag tggaaaagga tgtggggttg cacagacaac taggatgtta gcttagaagc
     3901 agctattcat tcaaagagtg cgtaatagct cactagtcga gtgaccctgc gccgaaaatg
     3961 taccggggct aaaacaattt accgaagctg tggatacctt tataggtatg gtaggagagc
     4021 gttctatgtg tgatgaaggt ataccgtgag gagtgctgga acgcatagaa gtgagaatgc
     4081 cggtatgagt agcgaaagac aggtgagaat cctgtccacc gtaagactaa ggtttccagg
     4141 ggaaggctcg tccgccctgg gttagtcggg acctaaggag agaccgaaag gtgtatccga
     4201 tggacaacag gttgatattc ctgtactaga gtatgtagtg atggagggac gcagtaggct
     4261 aactaaagca gacgattgga agagtctgtc taagcagtga ggtgtgaatt gagtcaaatg
     4321 cttaattcta taacattgag ctgtgatggg gagcgaagtt tagtagcgaa gttagtgacg
     4381 tcacactgcc aagaaaagct tctagcgttt aaacatactc tacccgtacc gcaaaccgac
     4441 acaggtagtc gaggcgagta gcctcaggtg agcgagagaa ctctcgttaa ggaactcggc
     4501 aaaatgaccc cgtaacttcg ggagaagggg tgctgactta aagtcagccg cagtgaatag
     4561 gcccaagcaa ctgtttatca aaaacacagc tctctgctaa atcgtaagat gatgtatagg
     4621 gggtgacgcc tgcccggtgc tggaaggtta agaggagtgc ttagcgtaag cgaaggtatg
     4681 aattgaagcc ccagtaaacg gcggccgtaa ctataacggt cctaaggtag cgaaattcct
     4741 tgtcgggtaa gttccgaccc gcacgaaagg cgtaatgatt tgggcactgt ctcaacgaga
     4801 gactcggtga aattttagta cctgtgaaga tgcaggttac ccgcgacagg acggaaagac
     4861 cccatggagc tttactgcag tttgatattg agtgtctgta ccacatgtac aggataggta
     4921 ggagtctaag agatcgggac gccagtttcg aaggagacgc tgttgggata ctacccttgt
     4981 gttatggcca ctctaaccca gataggtgat ccctatcgga gacagtgtct gacgggcagt
     5041 ttgactgggg cggtcgcctc ctaaaaggta acggaggcgc ccaaaggttc cctcagaatg
     5101 gttggaaatc attcgcagag tgtaaaggta taagggagct tgactgcgag agctacaact
     5161 cgagcaggga cgaaagtcgg gcttagtgat ccggtggttc cgtatggaag ggccatcgct
     5221 caacggataa aagctaccct ggggataaca ggcttatctc ccccaagagt tcacatcgac
     5281 ggggaggttt ggcacctcga tgtcggctcg tcgcatcctg gggctgtagt cggtcccaag
     5341 ggttgggctg ttcgcccatt aaagcggcac gcgagctggg ttcagaacgt cgtgagacag
     5401 ttcggtccct atccgtcgcg ggcgtaggaa atttgagagg atctgctcct agtacgagag
     5461 gaccagagtg gacttaccgc tggtgtacca gttgtcttgc caaaggcatc gctgggtagc
     5521 tatgtaggga agggataaac gctgaaagca tctaagtgtg aaacccacct caagatgaga
     5581 tttcccatga ttatatatca gtaagagccc tgagagatga tcaggtagat aggttagaag
     5641 tggaagtgtg gcgacacatg tagcggacta atactaatag ctcgaggact tatccaaagt
     5701 aactgagaat atgaaagcga acggttttct taagttgaat agatattcaa ttttgagtag
     5761 gtattactca gagttaagtg acgatagcct aggagataca cctgtaccca tgccgaacac
     5821 agaagttaag ccctagaacg ccggaagtag ttgggggttg ccccctgtg
//
LOCUS       DAVKQL010000089         5822 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVKQL010000089 DAVKQL010000000
VERSION     DAVKQL010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5822
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.089"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            1..243
                     /locus_tag="VW561_001973"
                     /pseudo
     CDS             1..243
                     /locus_tag="VW561_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(412..965)
                     /locus_tag="VW561_001974"
                     /pseudo
     CDS             complement(412..965)
                     /locus_tag="VW561_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1041..1454)
                     /locus_tag="VW561_001975"
     CDS             complement(1041..1454)
                     /locus_tag="VW561_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9944181.1"
                     /translation="MAHSFDSASIIAHTIHQKFNLKVPNYRQEEDWARMGLPITRKEI
                     SNWHIKASQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYWTFLSGKAEK
                     QGITLYHYDQWRSGSVVQEFLGDYSGYVHCDMLRQ"
     gene            1472..1779
                     /locus_tag="VW561_001976"
                     /pseudo
     CDS             1472..1779
                     /locus_tag="VW561_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1934..2257)
                     /gene="tnpB"
                     /locus_tag="VW561_001977"
     CDS             complement(1934..2257)
                     /gene="tnpB"
                     /locus_tag="VW561_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU9944182.1"
                     /translation="MTIHLSSLGQVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKVLYWDGQGFWLLYKRFENGKLTWLSTEKDIKALTSEQVDWLMKGFS
                     ITPKI"
     gene            complement(2238..2424)
                     /locus_tag="VW561_001978"
                     /pseudo
     CDS             complement(2238..2424)
                     /locus_tag="VW561_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2477..2617
                     /locus_tag="VW561_001979"
     CDS             2477..2617
                     /locus_tag="VW561_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutT"
                     /protein_id="HEU9944183.1"
                     /translation="MNDKYLVMTRLLFYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKK
                     IF"
     gene            complement(2614..3129)
                     /locus_tag="VW561_001980"
     CDS             complement(2614..3129)
                     /locus_tag="VW561_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEU9944184.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGVHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(3141..3668)
                     /locus_tag="VW561_001981"
     CDS             complement(3141..3668)
                     /locus_tag="VW561_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9944185.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            complement(3950..4513)
                     /locus_tag="VW561_001982"
     CDS             complement(3950..4513)
                     /locus_tag="VW561_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9944186.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILSLAIVPRLQTLKK"
     gene            complement(4497..5048)
                     /gene="coaC"
                     /locus_tag="VW561_001983"
     CDS             complement(4497..5048)
                     /gene="coaC"
                     /locus_tag="VW561_001983"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEU9944187.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLSLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            complement(5060..5749)
                     /gene="coaB"
                     /locus_tag="VW561_001984"
     CDS             complement(5060..5749)
                     /gene="coaB"
                     /locus_tag="VW561_001984"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEU9944188.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISADQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
BASE COUNT         1817 a         1092 c         1179 g         1734 t
ORIGIN      
        1 ccttatcttg aaacgactgg tatgcctact agaattctcc ttagaaagcg tcgattcaag
       61 tgctatcact gttcaaaaat gatggtcgct gaaacttcta tcgtcaagaa gaatcaccaa
      121 atccctcgta ttatcaatca aaaaattgct caaaagttaa ttgaaaagat ttctatgact
      181 gatattgccc atcagctttc catctcaact tcaactgtta ttcgtaagct caatgacttc
      241 cacgtttacg agagtttcct cgtttggaag acattctaga agataggata gatatttctc
      301 gctatttata atggattgaa ataagatatg aacaaattga ttaggaaagg aaaattgatt
      361 tatagaaatg ttttagcagt cgatgcgtac tgttatagat tcaacgcact ataattgatg
      421 acgtttagct gtttccaaca agctcataat aatagctctt gctttagctc cttcaaaact
      481 ttgagaaaat aaccaccctc gcttttttaa actgagctca ggctaaaaga gtccactgga
      541 ctcttttact ccgtcccata accaatgatt taatggcgcg ttcagctaga ttattggaaa
      601 ggaccagatg tccgtctttt aaaatagtct taaaggtttc tttatacttg aggctgtatt
      661 caattgccct tcctagtttt gaacctgcta aaactgactg acgacggcac caagcaaaga
      721 agtcttccat taggggttgg agctcttctt gacgtttctg tagtcgttca tcagctgaca
      781 atgtcctccc agtctctttc caaggcaaat aactgatcac aataagctaa acctttagct
      841 cctaaggatg atttatctgc ttgcttgggg gtcgcttcaa aaaacttcct tctcacatgt
      901 acccaacatc caacaagttc agcttcttcc agttgacaat aagcttctag cggttcgcag
      961 tttaccaagc ttagcgtcgc cttgctccta aaccttggac tgctatcgca taggcagaac
     1021 tagaggacta aagtcctaag ttactgccgc aacatatcac aatgcacata gccagaataa
     1081 tctcctagga attcttgtac taccgaacca cttcgccact gatcatagtg gtaaagcgta
     1141 atcccttgtt tctctgcttt acctgacaaa aaagtccaat agtaagtcag ctgactatca
     1201 ctttctagaa cccgatagga ggtttcatcc gcatgaagta agggctgagt caatagtctc
     1261 tctcgcaaga ggttataaag gggctccaaa tagtattgac tcgccttgat atgccaatta
     1321 gagatttcct tacgtgtgat tggtaaaccc atcctagccc aatcttcttc ttggcgataa
     1381 ttgggtacct tcagattaaa cttctgatgg atggtgtgag cgataataga agctgagtca
     1441 aagctatgcg ccaaaggggc tttaggaata ggagctttca caagcttatc cagatgatta
     1501 tcttttactc gttatgaaca atgctatatg gcataaatca agtaccttaa agattccgac
     1561 taatattggc tttgcattta ttcctccata cacaccagag atgaacccat tgaacaagtg
     1621 tggaaagaga ttcgtaaacg tggatttaag aataaagctt ttcgaacttt ggaagatgtc
     1681 atgaatcaac tccaagatgt tatacaagga ctggagaagg aggtgataaa gtccatcgtt
     1741 aatcggagat ggactagaat gctttttgaa aacagatgag tataaaaaga aagtcctcat
     1801 ttcaatagaa atcacaactt tctgatggat ttatagtaaa atgaaataag aacaggacaa
     1861 atcgatcagg acagtaaaat cgatttctaa caatgtttta gaagcagagg tgtactattc
     1921 tagtttcaat ctactatatt tttggagtga tagaaaagcc cttcataagc cagtctactt
     1981 gttcagatgt gagagctttg atatcctttt ctgtacttag ccaagtcagt tttccgttct
     2041 caaagcgttt atatagtagc caaaatcctt gaccatccca gtaaaggact ttaaagcggt
     2101 ctttacgtcc accacaaaag agaaagactt gaccggagaa aggatccaat tcaaagtggg
     2161 ttttaaccag ataagccagt gaatcgattc cttgtctcat atcagttttc ccacacacga
     2221 gatagacctg ccctaggcta gatagatgaa ttgttataga gcaacacctt atccaaaagc
     2281 tgttctacca tttcgagatt gagagattga aaaaaaactt acttcaagct tgccaatacg
     2341 aattttaagc agaatatcat ttctcttttt agaatcaaaa ggacgagatt gtggaatctc
     2401 tacaggaaca ataggttgtg gcattaaaat atcctccaat agttttactt cactaatagt
     2461 atacaggagg agctccatga atgataaata ccttgttatg acgcgcttac tcttctatga
     2521 tatgctacca ttgatggaaa tgatggaagc tcccgacaag tcagagtttt tctaccctcg
     2581 ccgtacagaa gacgattggg aaaagaaaat cttctagtct tttactaaat aacctagctg
     2641 atccaaggcc tcctcgatat agtggaggtc ttgttgtgtt tcggcttcga ctaggtggta
     2701 atggacgcca tctgtcaatt cagatagagg tctaaagtca gaatgctcga cttgttctag
     2761 aaaatgttgc acatctctgc gacaagacag tttaagcaag gtttcaattt ctccataaac
     2821 gggatggtca atcaaggtat tctggacatg cccgccatta tcgacaatag caaggagttc
     2881 ctgaccgatt tcttcaactt catgtttcac tttgaaaagt ttgtgaacat aaggattggt
     2941 atcaatttgc ttatagatgt agccacgatt ggtggatagg ataggagcgc catcggctct
     3001 caaaattgca atgtcctgca caatgacctg acgtgtgaca tgaaaatgtt ccgccaaact
     3061 ttggccatta agggctttag gagcttcttt caagagttgg agcagggctt gtttgcgatc
     3121 ttttgtcata gcttttcctt ttaacggcgt tttcgaagca ctttatagac agctagtgct
     3181 aatgtatagt ctaccatact atggataatt gtgccaaatc caactagtac aaatagaaca
     3241 tagaacatgt tttctacatt ggtacccgaa gttgcgtaaa aaaggacaca ggctaatact
     3301 tcagcaaggg catgaacaac agccaaaaca aagttgaaaa cccaggaaga ttttggttta
     3361 tctagggtat cggggaattt ttgtaggtaa agagctccta aagcaccaaa agatatatgg
     3421 gaaaaagccc gaaaaacgat aaccatggga tagccagcca tcaaaaatcc aaaactagag
     3481 gctaggatga caaaaactgc catcaagggc gacaagaaca tggctataaa aatagcgatg
     3541 tggctcccca aagtatagga agcaggtgga atgacaatct tgaaaggcat aacaattgga
     3601 atcaaaatcg caatagccgt taaaagggct gtcattgtca taaattgtgt ctttttccgt
     3661 gtattcacaa gaatctcctt tttaactgca tatacactag tatggtacaa taaaccagac
     3721 aataaagcaa gaatttactt gggtttatag atcatttttt agttaaaagt tatagtagat
     3781 tgaaactaga atagtacacc tctacttcta aaacattgtt agaaatcgat ttgactgtcc
     3841 tgatcgattt gtcctgttct tatttcgttt tactatagta aagatttcat taaaaagaaa
     3901 ctgtatagag caaaatctcc accttcaggt ttgaaagcgg agattgtttt tattttttca
     3961 aggtttgtag tcgtggaaca atggctaggg atagaattgc agaaatgacc aattcagcaa
     4021 ttgaatttgt tgagataacg gttgccagaa gaagttggat atttccatta taaacatttc
     4081 caaataggaa gaagattcct ccaaggacaa agatagtatt tgtcaaggaa ccaagggccc
     4141 cagctaaaat cagaccagtc ttgtttttca tcagtttata gactaagtaa ggagttaaac
     4201 caatcaaaat acgtgggaca atggcaatga tagctgagta gatgtttccg tttggtacga
     4261 agggagagaa gaggtagctt gtcggtagaa tcgtaatcgt gttaaccgtc aagctaagta
     4321 atcccatcaa aaatccaagt gtaaccccaa ctcgtggacc ataaataatg ctggcaataa
     4381 tgacaggaat atgaacaatg gtcggtttga ttggaaatgg aaaaaggtta aagataagtg
     4441 agctcagaaa gtgtatcacg agcatggtag caaaaaagat agcaatgggt gcaatattag
     4501 agcgtttttt catcgatagt ttcctttatt ctttctaaaa taattgtgag gtcagctaaa
     4561 gctcctcgtc cgtggtctcc acaagctagt agggattcct taggagcaat cagctgatag
     4621 ccgtaggttt ctaatgtttt cagattattc tgagttactg gatggtcata catttttgta
     4681 ttcatagcag gagctattag tttgggaata tgacttggta gggctagagc tgtactggtt
     4741 accatgttgt ccgcaaagcc gtgagctagt tttgcaatag tgttagcagt tgcaggtacc
     4801 acgataaata aatctgcttt ttttccaagt tcgatatgat tgacctgatc aggatagggt
     4861 tccttcatga catccaagtg gacaggattc tgtgagagta cctgtagtga caaaggttgg
     4921 ataaactctg tagcagcctg agtcattaag acagtgactt gatggccttg tttttttaga
     4981 gaactgacta aatctgccga cttataagag gcgattgaac ccgttacagc caagagaatg
     5041 tttgccatag ttttcctttc taagaatgat aggcttgaat tttttcaagg aggagttctg
     5101 caatttcttc tttagtctgg actgtttgaa gctgattttt ctcaacaaat atagctcggt
     5161 gctgatctgc tgaaatttga gtcaggtcat tcgcgatgat taaatctgct tgattcttga
     5221 taagactttt tcgtgcaata tcaaccagat gatcttcggt aacatcaacc agcagtttga
     5281 aaccaatcag atgaatagta ggattccatt ccttgactag ggatatgatt ttgggtgtct
     5341 ttttaaggaa caaaacctga acctcatcag ttgaagaaat cttggcctga tgattctgct
     5401 tgcttaaaaa ttcttttaga ttggagctag cctgaacttc ctcaagccct gtcatataaa
     5461 caggagtgta gtcagaaaca gccattgagt ggatcaagac ctgataatcc tgaacacgtt
     5521 cttgcatttc tattagaagg tccttggtat tggtaatttc tcgaatactt aggttaggat
     5581 gaggctctgg cttcagagct cgttttgtcg taattaaaca aacttcatgc cctgcagaaa
     5641 gcaaagtctc tgtgataatt ttccccaagt gacctgtaga atggttagtg atagagcgga
     5701 cgctatcgat agcttcactg gtaccgcccg atgtaactaa aattttcata gcactattgt
     5761 acacaaaaat aagcggtaag taaaatgaaa gtgataaatt tttggtaaaa acgaagattt
     5821 ac
//
LOCUS       DAVKQL010000090         5686 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVKQL010000090 DAVKQL010000000
VERSION     DAVKQL010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.090"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(174..965)
                     /locus_tag="VW561_001985"
     CDS             complement(174..965)
                     /locus_tag="VW561_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944189.1"
                     /translation="MISSIKSHNILSELYKIFYSRSTQIVLPIVFILQPLLSYISSKQ
                     ILTVGLNATPETNSSLAESIPPIEYIGFDAILLGLFAMIILGAIIGSMEYKNSSLRTS
                     LLLCSNKAVFFVIKFFVTSVFIFVVSCVSIYLSIASAQFGLGREGLNPLILSNNVWYF
                     ILLGSLSWSLLTVLSYSIAFYFKSSLGSLLFLLPQVYNLGSFLSDRIQLAKYLPVSLG
                     QDLIATSPLKITTPGRSVLFLSIWVLVISSFAYYKFLKKDVGNVI"
     gene            complement(955..1671)
                     /locus_tag="VW561_001986"
     CDS             complement(955..1671)
                     /locus_tag="VW561_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9944190.1"
                     /translation="MTNKIEIRNVTKTYKDGDILKNVSFTAEEGEVTAFLGPNGAGKS
                     STLRILLGLDRSSLGTALIGGKKYCDIERPLLTVGASFDGVGAPSDRTVFQHLKIAAA
                     SNGIGFSRISEVLSITDIEHKRYSKISNLSLGEGQRLGIATALLGNPQYLILDEPTNG
                     LDPSGIRWFRNFIREQASTGKTVLLSSHILSEVEAVTDKVVFIDKGSIIASGTLHNIM
                     NGFDNLEDVFFHLTGDDYDK"
     gene            complement(1684..3906)
                     /locus_tag="VW561_001987"
     CDS             complement(1684..3906)
                     /locus_tag="VW561_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HEU9944191.1"
                     /translation="MDMQLNQESYSYVQLKYRLLAQIALLISYPLTKFSPKRIELLIN
                     KLSNSKLSATENEAILARDAICTVSHKCRNQDGCVKRSLGVIMFLLLLNKRASWCTGF
                     AMNPFRSHAWVEVNGNPIKELEEVSSYERVVKTLDDKNEERKHMISENIEKVDDTMTN
                     VKFRDLLVLIENKRKYFLIVLCLEILTSILTLLQPELLSKVISQKGVNLFNNPTIYML
                     ITITVGSTIISSLQYYFLQLMGESAVFQSRKNLIIQFLKLPIYKYNDLSAGDLISRFS
                     SDTSKLRAGIIQSTVALTSGLFLSLGAIFTLFLKDTYLAIITIISIALSFLFILLMSS
                     LIQTASYKAHQGLGKMTAYLNRLIVGIRTIRSTNETNSELSKMLSEVQEVKNLGIGVA
                     RVQSIMTPISNFSLQLCGIIILGIGGYRVSTGQMSIANLTSFMLLMYIAISPVGQIFS
                     SVTTISEALGALSRITEIIDLPKEEDNDIILNLESKSDRSKAIEFVNVTFSYDSYQFQ
                     DIQSDDNYILKDITFEINSGDYVSIVGPSGAGKSTLLYLIERFYDTTSGGIKIFGQDF
                     RTISRETLRSNISYVEQNSPLVAGTILENLKMGNSNVTYDECCDALEKVGLEHLIQRG
                     ASGIESPISEGGFNLSGGERQRLAMARAILSDAKILLLDELTSNLDSLSEKKMKEAIK
                     SMRGERTIIMVAHRLSTILDSDEIFVLEHGRIVGNGTHMELLESVPLYKELAKEQFLV
                     "
     gene            complement(3910..4161)
                     /locus_tag="VW561_001988"
     CDS             complement(3910..4161)
                     /locus_tag="VW561_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEU9944192.1"
                     /translation="MFNKESKSRLKIIKQDTGAIVFDKNQGIYFQTNDVGVKILELLS
                     KNESEEEIVSAISVAYSIEIDVAKQDVKDFIQSLKKGGL"
     gene            complement(4176..>5686)
                     /locus_tag="VW561_001989"
     CDS             complement(4176..>5686)
                     /locus_tag="VW561_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthetase"
                     /protein_id="HEU9944193.1"
                     /translation="VEMAERTYIISPYLDSRLSWFENNKNIIFSDSSVEIAKYLNLKL
                     STKRLASQFIFGLPYYPFQTISLWEELVNIRPLNYLEISEKGCLEKRVPSYEVDTKDF
                     NKLIIKIKEEFLNSINHDLNMRNEVSSDVSGGVDSATIAFTLNKLIPDFSILHAESST
                     TANSDTKWATFIAKSLGRELKKFDSIEITEKRFAIDEKYINDIMPSSPLLWADSEGYL
                     KSVIGYQKGRKQPTHFLGIGGDELFTPMPSNSWNIVRQENLGGLLYALKYSLIMRRPF
                     FSCLLDLLDNRGYSETVIKNLEIVFNESSAPIKRELGWVDGLQVPDWLSEKSKQESQI
                     FLNSLLFSNSEPIISDRTVFQMIQSLIFQKSVLRQIQLTTNSIYWATPFLHKKLIEIC
                     LQIPAKYKVSSKLTKPILQKALKGIVPIEVFNRGFKGDYSDALYSGYREAVRKNFHNL
                     EQFELVKMGIIDVEKLKLEMSLPAGNPSKIDYFEKLCSVERWIRQIKLYMKN"
BASE COUNT         1888 a         1069 c          750 g         1979 t
ORIGIN      
        1 ttgtccaaga tagaagcttt ctaagatttg cgttaaaaca taatcctcgg ttatataact
       61 tggagtttca tgtaaaaaaa taacaaataa aggcactaaa attatagttg ctaatacagt
      121 caataatagc catttattat aaataaactt taaatactca cttctaaaag aattcatata
      181 acattaccta catctttttt caaaaatttg taatatgcaa aactactaat tactagcacc
      241 caaatactca gaaataaaac actgcgtcct ggtgttgtga tttttaaagg agatgttgca
      301 attaaatctt gacctaaaga tactggtaaa tactttgcaa gctgaattct gtcagaaaga
      361 aaactgccta aattatagac ttgtggtagt aagaataata atgatccaag tgaggattta
      421 aaataaaaag caattgaata ggataatacc gttaataacg accacgacaa tgaccccaat
      481 aatataaagt accacacatt attactcagt atcagagggt tcaatccctc tctccctaaa
      541 ccaaactgtg cacttgcaat agataaatag attgaaacac acgatacaac aaaaataaat
      601 acacttgtaa caaaaaattt tattacaaag aatactgcct tatttgaaca taatagcaaa
      661 ctggtacgta aagaactatt tttatattcc atacttccaa taattgctcc taaaataatc
      721 atagcaaaca agcccaataa tattgcatca aaaccaatat actcaattgg aggtattgat
      781 tctgctaaac tactatttgt ttcaggtgtt gcattcaatc caactgttaa aatttgttta
      841 gaactgatat aagacaataa cggctgaaga ataaaaacaa ttggtaaaac tatttgggtt
      901 gatctactat aaaaaatttt atatagctct gataaaatat tatggctttt tatactactt
      961 atcatagtca tctcctgtta aatgaaagaa aacatcttct aaattatcaa atccgttcat
     1021 tatattatga agtgtacccg aagctatgat acttcctttg tcaataaaaa caaccttatc
     1081 agttaccgcc tctacttctg aaagtatatg tgaagataac aatactgtct tgccagtact
     1141 tgcttgttct ctaataaaat ttctaaacca tctaattcca ctaggatcta aaccatttgt
     1201 aggttcatct aaaattaggt actggggatt tcccaacagc gctgttgcaa tacctaacct
     1261 ctgaccctct cctaatgaaa ggttgcttat ttttgaatat cttttatgtt caatatcagt
     1321 aatggataat acctctgata ttctggaaaa acctattcca ttactagcag cagcaatttt
     1381 caaatgttga aaaactgtcc gatcactggg agcccctacg ccatcaaaag aagctcctac
     1441 agttaaaaga ggacgttcaa tgtcacaata tttcttacct cctattaaag cagtacctaa
     1501 agatgatcta tccagcccca aaagtattcg tagtgtcgaa ctttttcctg caccattcgg
     1561 tcccaaaaag gccgtgactt ccccctcttc agctgtaaag gatacgttct ttaatatatc
     1621 cccatcttta taagtttttg taacatttct tatttcaatc ttatttgtca taatacgata
     1681 ctcctatact aaaaattgtt ctttggccag ctctttatat aatggaacag attccaataa
     1741 ctccatatga gtcccatttc ctacaattct tccgtgttcc aaaacaaaga tttcatctga
     1801 atctaaaatt gtagataatc tatgagcaac cattattatg gttctctcac ctctcatact
     1861 tttgattgct tccttcattt tcttctcact cagactatcc aaattcgaag ttaactcatc
     1921 cagcaagagt atctttgcat cggaaagtat tgctcttgcc attgccaatc tctgtctttc
     1981 tcctcctgat aaattgaacc ctccttcact aattgggctt tcaatcccag atgcacctct
     2041 ttgaattaaa tgctccaaac caactttttc taaagcgtca caacattcat catatgttac
     2101 attactatta cccattttta gattttctaa gattgtacca gctactaatg gtgaattttg
     2161 ttcaacatat gaaatattcg aacgtaaagt ctcacgactt attgttcgaa aatcttgtcc
     2221 aaatatcttt ataccaccac tggtagtatc atagaatctt tcaatgaggt aaagaagtgt
     2281 acttttcccc gcaccagacg gaccgacaat actgacgtaa tctccactat ttatttcaaa
     2341 agtaatgtcc tttaatatgt aattatcatc cgattgaata tcttgaaatt ggtaagagtc
     2401 atatgaaaaa gttacattca caaattcaat tgctttgctt ctgtctgact tagattctag
     2461 atttaaaatg atatcattat cttcttcttt tggaagatca ataatttcag taattctact
     2521 taaggctccc aaagcttctg aaatagttgt gactgacgaa aatatttgtc ctactggtga
     2581 aatagcgata tacattaata gcataaaaga agttagattt gcaatagaca tttgcccagt
     2641 agaaacacgg tagcctccaa ttcccaaaat aattatccca cacaattgca agctaaaatt
     2701 tgatattggt gtcattattg attgcactct tgcaactcca attcccaagt tttttacttc
     2761 ttgtacctca gataacattt tagataattc tgaattcgtt tcatttgtcg accgaattgt
     2821 tctaatccct acgattagcc tatttaggta tgctgtcatt ttccccaaac cctgatgagc
     2881 tttataactt gccgtttgaa tcaaactact catcaataaa ataaatagga atgataaagc
     2941 tatagaaata attgtaatta ttgcaagata agtatccttt aaaaatagtg taaatatcgc
     3001 acctaaactc aagaacaatc cactcgtcag agcaacggtt gattgaatga tacctgctct
     3061 tagtttactt gtatcactgc taaagcgact aattaggtct cctgcagata gatcattata
     3121 cttataaata ggaagtttca aaaattgaat aattaaattt tttcttgatt gaaatactgc
     3181 actttctccc attaattgta aaaagtaata ttgcaaactg gaaataattg ttgaacccac
     3241 ggtaatagtt ataagcatat atatggttgg attgttgaat aaatttactc ccttttgaga
     3301 gattacttta gataaaagtt ctggttgtaa taacgttaaa atggacgtaa gtatttccaa
     3361 acataaaacg attaaaaaat attttctttt attttctatc aaaacaagta aatcacggaa
     3421 ttttacgtta gtcatagtat catctacttt ttctatattt tcagatatca tatgtttacg
     3481 ctcttcattt ttatcatcta gagttttcac gactctttca tacgatgata cttcttctaa
     3541 ctctttaatc ggatttccgt ttacttccac ccatgcatga gatctaaagg ggttcatagc
     3601 aaatccagta caccaacttg cacgtttatt taaaagaagt aaaaacatta ttactcctaa
     3661 tgatcttttt acgcatccat cttgattcct acacttatgg cttacagtac atattgcatc
     3721 acgagctaat attgcttcat tttctgtggc agatagtttc gagttactta acttattaat
     3781 caaaagttca attcttttag gcgagaactt tgtaagcgga taagaaatca gtaaagctat
     3841 ttgtgctagt aatctgtatt ttagctgaac ataggagtat gactcttgat tgagttgcat
     3901 atccataatc tatagccctc ctttttttag agattgtata aaatctttaa catcttgttt
     3961 tgcaacatcg atttcaatgg aatacgcaac agatatagcg ctaacaattt cttcttcgct
     4021 ttcattttta gatagtaact ctaaaatttt gacacctaca tcatttgttt gaaagtatat
     4081 cccttgattt ttgtcgaata caattgcacc agtatcttgc tttattattt tcagtctact
     4141 tttcgattct ttattaaaca taattgtaaa cctcactagt tcttcatata taatttgatc
     4201 tgccgtatcc aacgttctac cgaacataac ttttcaaaat aatcaatttt actcggattt
     4261 cccgcgggta atgacatctc taattttaac ttctcaacat caataatacc cattttaacc
     4321 aattcaaact gttctaaatt gtggaagttt ttacgaactg cttctctata tccactatat
     4381 aaggcatctg aataatcccc tttgaaaccc ctgttaaaaa cctcaattgg tacgattccc
     4441 ttcaatgctt tttgcaatat cggttttgtt agttttgaac taactttata ttttgctgga
     4501 atttgaagac aaatttctat taatttttta tgtaaaaatg gagttgccca ataaatggaa
     4561 ttagtggtta attgaatttg tcgcaatact gatttttgaa atattaagga ttgaatcatt
     4621 tggaagactg tcctatcaga tattattggc tcagaattgg aaaataataa cgagtttaaa
     4681 aaaatttgcg attcttgttt ggatttttca ctgagccaat ctggtacttg taaaccatcc
     4741 acccatccca gttctctctt gataggtgca gaagattcgt tgaaaacaat ttctaaattt
     4801 ttgatcacag tttccgaata tcctctatta tctaacaaat ccaataggca actaaaaaat
     4861 ggtcttctca ttattaaact atatttcaga gcataaagta gtccacctaa attttcctga
     4921 cgaacaatat tccaggaatt agacggcatc ggtgtaaata attcatcacc accaatacct
     4981 agaaagtgag tcggttgttt tctacctttc tggtaaccaa tcactgattt caaatatcct
     5041 tctgaatccg cccacaataa cggagaactt ggcataatat cattgatata tttttcatct
     5101 atcgcaaatc ttttttctgt tatttctatt gagtcaaact ttttcaactc cctaccaaga
     5161 cttttagcaa taaatgtcgc ccatttagta tcactattag cagtggtact tgattcagca
     5221 tgcaaaatag agaagtcagg tatcagttta ttcaatgtaa aagcaatagt tgcagaatca
     5281 acacctcctg atacatcact cgacacttca tttctcatat tcaaatcatg attaatgcta
     5341 tttaaaaact cctcttttat ttttattatt agtttattaa aatcttttgt gtctacttca
     5401 taactcggaa ctcttttttc taaacaacct ttttcagata tttctaaata gtttaaaggt
     5461 cttatattca ctagctcttc ccagaggcta attgtttgaa atggataata tggaagtcca
     5521 aaaataaatt gacttgctaa acgcttcgta gagagtttta aattcaaata ttttgcaatt
     5581 tctacagatg aatcactaaa aattatattc ttattattct caaaccaaga cagtcttgaa
     5641 tctaaataag gtgaaataat atatgttctt tcagccattt caacaa
//
LOCUS       DAVKQL010000091         5455 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVKQL010000091 DAVKQL010000000
VERSION     DAVKQL010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5455
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.091"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            112..360
                     /locus_tag="VW561_001990"
                     /pseudo
     CDS             112..360
                     /locus_tag="VW561_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            491..1663
                     /locus_tag="VW561_001991"
     CDS             491..1663
                     /locus_tag="VW561_001991"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEU9944194.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            1663..2937
                     /locus_tag="VW561_001992"
     CDS             1663..2937
                     /locus_tag="VW561_001992"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEU9944195.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(2972..3937)
                     /locus_tag="VW561_001993"
     CDS             complement(2972..3937)
                     /locus_tag="VW561_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9944196.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(3918..5372)
                     /locus_tag="VW561_001994"
     CDS             complement(3918..5372)
                     /locus_tag="VW561_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU9944197.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
BASE COUNT         1528 a         1108 c         1258 g         1561 t
ORIGIN      
        1 tgaatttgag tgctggtatt atcaatcatg aactaggatt ctagttttgc aggaagtcta
       61 ctactttcct tattgtctgt aaatctactg accttgttgt ttatcccagt catggtttct
      121 agttcgggct cagagtttca aagtggatgg taagagcatc aattgattgc tgagaaggtt
      181 agtaaaacac ttgacaagac atttgataag gatgtcagag aaattccgac cagtcagttt
      241 tatcaaaaat ttgtagatga gatgggaagg acttactcag gaaatttgat cctccaggag
      301 ctgataactg tgaatggagc ttataaagct acttatatcg gtgaactctc tagcaactaa
      361 tcattcttta aaatgatgac caataagagg cttgatttgg tgaatcaagt cttttttctc
      421 tcttttactg ccatttgtga taaaatagta tagaacgatt ttttacatga atgataaaac
      481 agaggtaaat atgacaatcg gtattgataa gattggtttt gcgaccagtc aatatgtctt
      541 gaaattacaa gacttagcag aagcgagggg aattgaccct gaaaaattaa gtaaaggact
      601 cttactcaag gaattgagta ttgcgcccct aactgaggat atcgtgacct tggcggccag
      661 tgctagtgac tctattttaa ctgagcaaga aagacaagaa gttgacatgg tcattgtggc
      721 gaccgagtca ggaattgacc agagtaaggc tgcggccgtc tttgtgcatg gcttgctggg
      781 catccagccc tttgctcgta gtttcgagat taaagaagcc tgctatgggg cgacagctgc
      841 cctccattat gccaaattgc atgtggaaaa ttctccggag tccaaggtct tggtcattgc
      901 cagtgatatt gccaaatatg gtattgaaac tccgggagaa cccactcagg gtgccggaag
      961 tgtggctatg ttgattacac aaaatccacg catgatggcc tttaataatg acaatgtagc
     1021 tcagacccgt gacatcatgg atttctggcg accaaattac tcgacaactc cttatgtaaa
     1081 tggtgtctat tctacccaac aatacttgga tagtttgaaa acgacttggc ttgaatatca
     1141 aaaacgctac cagcttactc tggatgattt tgcggctgtt tgtttccact tgccttatcc
     1201 taaattagcg ctaaaaggct tgaaaaaaat catggataag agcctgcctc aagagaaaaa
     1261 agacctctta caaaagcatt ttgaccagtc tattctctac agtcaaaagg tggggaatat
     1321 ctacacaggt tcacttttcc ttggactttt gtctctcttg gaaaatacag atagcttgaa
     1381 agctggggat aaaatcgccc tttatagtta cggaagtgga gctgtggctg agttcttcag
     1441 tggtgaattg gttgaaggat atgaagctta tttggataaa gaccgcttga acaagctcaa
     1501 ccaacgaact gccctatccg ttgcagacta tgaaaaggtc ttttttgagg aagtaaactt
     1561 ggatgaaaca aactctgccc agtttgctgg ctatgaaaat caagattttg ccttggttga
     1621 aattcttgac caccaacgcc gttatagcaa ggttgaaaaa taatgaagat aagttggaat
     1681 ggattttcta aaaaatcata ccaagagcgc ctcgagctgc taaaagctca ggcgctcctt
     1741 agtcctgaga gacaagctag tctggagaag gatgaacaga tgagtgtgac tgtggcagac
     1801 cagctgagtg agaatgtggt gggaactttt tctctgcctt attcgctggt tccggaggta
     1861 cttgtcaacg gtcaggaata caccgttccc tatgtgacag aagaaccctc tgtggttgcg
     1921 gcggccagct atgccagcaa aatcatcaag cgtgcaggtg gttttactgc acaagtccat
     1981 cagcgccaga tgattgggca ggtagccctt tatcaagttg ctaatcctaa actagcgcaa
     2041 gagaagattg ccagcaagaa agcggagctc ttggagcttg ccaatcaagc ctatccttct
     2101 atcgttaaac gtgggggtgg ggcgcgtgat ctgcatgtcg agcagataaa aggcgaacca
     2161 gactttctcg ttgtttatat tcatgtcgat acccaggaag ccatgggtgc caatatgctc
     2221 aacaccatgc tggaagcctt gaaaccagtc ttagaagaac tcagtcaggg acagagtctc
     2281 atgggaatcc tgtccaacta cgcgactgat tctctggtga ctgcaagctg tcgcatcgcc
     2341 tttcgctact tgagccgcca aaaggatcaa ggacgagaga ttgcggagaa aattgcgttg
     2401 gctagtcagt ttgcgcaggc tgatccttac cgagctgcta ctcataataa aggaattttt
     2461 aatggtattg atgcgatttt gattgccact ggtaatgact ggcgtgccat cgaagctggg
     2521 gcccatgcct ttgccagtcg agatggacgc tatcaaggtc ttagctgctg gacgctggac
     2581 cttgaaagag aagaattggt cggtgagatg accctgccca tgcctgtagc gactaagggt
     2641 ggctctatcg gcctcaaccc acgtgtagct ctcagtcatg atctactagg aaatccttct
     2701 gccagagaat tagcccagat tatcgtgtcc atcggtcttg ctcaaaattt tgcagccctc
     2761 aaagccttgg taagtacggg catccagcaa ggccacatga aactacaggc caaatcccta
     2821 gctctcctag ctggggctag tgaatctgaa gttgctcccc tagtagagcg cctcatctca
     2881 gataaaacct ttaacctaga gacagcccag cgctatctcg aaaatttaag atcataaaaa
     2941 ctcagacgaa tcggtctgag ttttcttgtg tttaaatact ttttcctggt aatagtgtaa
     3001 ctggtaagaa gtaaccagtt gtggcgactt ccttgccttc aatcttttgc aagagaagat
     3061 caatagtgag acaagcaatc tcttccaaag gttgcttgat agtagccaat tgagggtagt
     3121 aattttcgat aaagtaggtc ccatcatagc cgatgacctt gagctctttt gggacagaaa
     3181 tgcccaattc ttgagcgatt ttaatgacca gaatagctgt caaatcatcc gaagcaaaaa
     3241 tggcatctgg tttttcccgg gtcaagatat tcttgatttc catttctttt ctgacgggag
     3301 aaaagtcact ggaaacattg ataataggag cttttgggag tacggatgca aaaccagcgt
     3361 ggcgcagtcc ggttggcgaa ttggaattgt cattccctgt aatcatgatg atagactggg
     3421 cacctgtctt gaccaaggtt tgggcagcaa gaaccccacc agcatagttg tcagaggaga
     3481 caacagggat gtctggcgat aggtttcggt caaaggaaat aatcggcgct gtcacacgat
     3541 tgtagtcttc gattcctagg ttgtgactac cagaaatgat gccgtctacc tgattggctt
     3601 ccaacatttc gatgtattcg cgttccttct cagaatcatg ttcactgttg cagatgatgg
     3661 tcttgtaacc atttttgaag agttggtgtt ccaatttatc aatcaattct gcatagaaaa
     3721 cattggaaat attggggaaa atcaagccga ttaacttagc tgattttcct tgcagactac
     3781 gagccaggtt gttgggttta tagcccaatt ctcgcatggc ttcattgact ttttggatgg
     3841 ttttctcaga tagataccct tttttattga taacccgaga aacggtagta ggactgacgc
     3901 ctgcaagttt ggcgacatca gttagttttg cgaccataat ctaattcata gtaagttcca
     3961 gttgggtttc cagatttgat caggatacca ttttggtccg catgtgggaa gacacgacca
     4021 gaaaatactt tttctccttt attgatgaaa atttcaaaga cagagttatc gatgaagatt
     4081 gtagcagtag tagcctgatt ctcgataggg caagaacgag ttgtcccaaa ttcttgggca
     4141 tactgttctc cagcctggct acgatccact gtcacttgac cgtttacaag gtcaaagttg
     4201 attgaaagtc ccttaccttc tttatcagca agtaagacaa tctcgctctg gctattagct
     4261 tccaagttga gttcaagttc gtaagtgttc ttggtttggg aacggtttga gaaggcttct
     4321 tcagaagcac gaaggtcctt aatagcagcg actggatact ggtagagctt gtcgtctttg
     4381 atagtgagtt ccttgaccaa agagaaggtt ccttggtggt caaaacggtc agatgggtaa
     4441 gaaacatctg gcaaaccaag ccagctaact gctagagcac gcccatcagg agcgttgaag
     4501 gcttgagttg cataggcttc gaaaccgtaa tccatgtttt gaagttgaga cacatctacc
     4561 attttggcat ttttagggtc aaaggaagcc ccaatcttat acatatttgg aaagatatta
     4621 tcgtagtcta gaactttctt atccaatcct tgtggacagt agagaaggac aggctgttcc
     4681 tctacaaaga ccagattagg acattccatc atgtaggcag tacgatcgtt agcaaagtca
     4741 aggtcgccaa ctgcttgcca gtttgtgtag tcgttattga cagccttgta gagacggacg
     4801 aagccttttt tctccaagtc ctgtccaccg acgacagcat agtattgacc tttaaagtta
     4861 aaaatttgtg gatcgcggaa gtggtcagta gagtctgctg gctggtcaat caagatcttg
     4921 tcaatctttg taatcttacc ctccttgtcc atcaaagcac cgatttggta tgggtgacgg
     4981 atccagtttt catcgcgaac atttcctgta taaaatagga ataagttatc gccaaattgc
     5041 atggcagaac cagagtaggc accgtggcta tctaatggag tatctggtaa aactttgatt
     5101 ccagtttctt taaagtgaac caaatcatca ctttctagct gtgcccaaga tttcaaaccg
     5161 tgggctgcac caaaaggaaa attctggtaa aagaggatcc acttgccatc aaagtaagaa
     5221 aagccatttg ggtcgttgag aagtcctgtt tttggctcaa catggtaatg agtatgccat
     5281 ggagattgtg ccatattttc ctttatatgt tgaatttctt cttgcgtcca atcttgataa
     5341 agtctgtaac gacgctcagt tgtccattcc attcattcct ccaaaaatct tatcactttt
     5401 aatagtaact gttttcgata aaaaatgcaa gccttttatg ttaaaaaaga ggaaa
//
LOCUS       DAVKQL010000092         5263 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVKQL010000092 DAVKQL010000000
VERSION     DAVKQL010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.092"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(409..1137)
                     /locus_tag="VW561_001995"
     CDS             complement(409..1137)
                     /locus_tag="VW561_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9944198.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRYSS"
     gene            1295..2704
                     /locus_tag="VW561_001996"
     CDS             1295..2704
                     /locus_tag="VW561_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU9944199.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLELDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWLERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            2722..4017
                     /locus_tag="VW561_001997"
     CDS             2722..4017
                     /locus_tag="VW561_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9944200.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            4022..4330
                     /locus_tag="VW561_001998"
     CDS             4022..4330
                     /locus_tag="VW561_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9944201.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            4327..4635
                     /locus_tag="VW561_001999"
     CDS             4327..4635
                     /locus_tag="VW561_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9944202.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1779 a          881 c          952 g         1651 t
ORIGIN      
        1 tgattcagaa tttattcaat atattaaaac aatatttgaa caaataaatt gggagattga
       61 aattcatttc tagcaatttg ttagaagtca cagtctacta ttctaacctc agtatactat
      121 atgtgttaca agaaaagact caaaaacttc tcgtcagtgt aagaatattt gaaacactat
      181 ttaaaactaa ataactataa gaagtagatc aggtgaacaa tttcagtacc tactctataa
      241 gctatacgaa gatatttttc aacatgatgg ataaaacgat cactctttta aagccatctt
      301 atttagaaaa gagaaaccat ttaagcatag atgggacctt cttgccctgt tttaaatttt
      361 tcttaaacta gagtctgtaa aagattggct tcattttcac attttctcct aggaggaata
      421 tcgtaatgca aaagaattaa attcaaataa atcatagcga tgacgactaa tcgagtactc
      481 aaacggagtt ccgttatcca aataagccac ttgttctact tcaaataccg gttccgttgg
      541 aaggagctgc aagtaatgtt gctcattttc tgaactagaa gaagctcgta agatttttgt
      601 agcactggca atatgcaatc ctaactttcc ttcaatgtaa ttgtaaatcg atttttgtaa
      661 aatatcttca gtaatacctg gaataacatc ggtactcata taagtttgtt ccagaacata
      721 aggtttaccg tcaataatac gcaggcggta aatattataa actggactct gcaaagcgac
      781 ctgtagtttt tctgctaaaa attcatttgc aaattctaat ttgaagtgaa taatcctcga
      841 gtcaattgta gaatgagcat cttcagatat ttttgtcagt ccccggatat ctctttctgg
      901 aacgattaat tttctttttg agctgccacg agagagaaca aaggttccct gcccacgttt
      961 tctaaaaatt aagccctcag aaactaacaa gtctaaagct tttttgatcg tcattcgact
     1021 acaggcaaac tgtttagcta atgtcatttc atcaggtaat tgttcgttca tggtatattt
     1081 attacaaaga atcttttcac gaatctcatt gtaaataaat aaatattttg tactcattta
     1141 atctggttct ctctataata ttctaatagt agtataacaa agttcaagaa atgaggcaaa
     1201 cacaagatat gtatcaacaa tttttaaaaa tgtctagaca aattttgaaa ttatgctata
     1261 ctttatgtat agaataatag aaaggaggtt gagcatgaaa aaaaagttag tatttcctaa
     1321 tctgttttgg tggggagcag cttctagcgg acctcagaca gaaggtcaat atggaaaagt
     1381 acatgaaaat gtgatggact actggttcaa aacgcatcca gaagattttt tcgataatgt
     1441 cggacctctt gtagccagta acttttttca tacttacacc gaagatttcc acttgatgaa
     1501 ggaaattgga gttaattctt tccgcacttc catccaatgg agtcgactca tcaagaattt
     1561 agagacaggt gagcctgatc caaaaggtat tgctttctac aatgccatca ttgaagaagc
     1621 taaaaagaac cagatggatc ttgtgatgaa tttacatcat tttgatttac cagtggaact
     1681 tcttcaaaaa tacggtggtt gggaaagcaa acatgtagtg gagttattcg tgaagtttgc
     1741 caagactgct ttcacatgct ttggagataa ggttcattac tggacaactt tcaatgagcc
     1801 aatggtcatt ccagaagcag gatacttata tgctttccat tatccaaatc taaaaggaaa
     1861 gggaaaagag gccgtacaag tcatctataa tctaaacctt gctagtgcaa aagtgattca
     1921 actatatcgc tcattagaac ttgatggaaa gattgggatt attttaaact tgacacctgc
     1981 ttatccaaga agtaattctc cagaagactt agaagcaagt cgatttacag atgacttctt
     2041 taacaaagtc ttcttgaatc cagctgttaa aggaactttc ccagaaagat tggtaaaaca
     2101 gctagagaga gatggcgtgt tatggagtca taccgaaaaa gagcttcaac tgatgaaatc
     2161 aaatacggtt gattttcttg gagtaaacta ctaccatcca aaacgtgttc aagcacaagc
     2221 aaatcctgag gaatatcaga cgccctggat gccagaccaa tacttcaaag agtatgaatg
     2281 gctggagcgt cgcatgaatc catatcgtgg ttgggaaatt tttccgaaag ccatttatga
     2341 tattgctatg attgtgaagg aagaatatgg taatatccca tggtttatca gtgaaaacgg
     2401 aatgggtgtt gaaaacgaag cacggtttat cgatgaaaat ggagttatcg atgacgtgta
     2461 tcgtattgaa ttttatgaag aacatttaag atggctacat aaagccattg aagagggaag
     2521 tcactgtttt ggataccacg cttggaccgc atttgattgc tggtcttgga ataatgcata
     2581 taagaatcgt tacggattta tctccgttga tttagaaacg caaaagagaa ccatcaagag
     2641 ctcaggaaga tggtatcgca aagtaagtga caataacggt tttgaagtag aaattgagga
     2701 gtaaagagag gagatattca tatgaaaaac tcaaaattta tagaccaatt tgccaccttt
     2761 gctggtaaac tagggaacca aattcattta aaaaccctaa gagatgcatt cgtaacagta
     2821 atgccattat atattttggc aggtttaatc gttcttttga acaacacggt atttaagtgg
     2881 attttccaag gggatacatt aacaagattc caatattggg gaataacaat tgcaaacggt
     2941 actttaagta tttcaggtat gattattgct gtaatggttg gttatttctt agctaaaaac
     3001 agagatttcg aaaacccgtt agcagcatca atgctatcat tagtttcttt aattgtgatg
     3061 atgccaaata cagtttctgt agttcctgac ggagcaaaag atgctgtaaa catttcaggt
     3121 gttctttcat tcaacaacac aggtacaggc gcaatgttcg ccggggttat cgtagcgatt
     3181 attgcaacag aattattcat tgaattatca aatgttaaag ctttacaaat gaaccttggt
     3241 gaaaatattc caccagctgt tagtagatca tttagcgtat tacttccagt catgaccgtc
     3301 atctccttat ttggggttgt ttcagcatta ttgttcaata taactggaat gaacttaatc
     3361 tcaatcatta caatctttat tcaagaacca attcgtcata ttggtacaag cttaatcggg
     3421 gtcattatta tttactcttt aggaaatatg ttatggctat ttggtattca ccaagcagtt
     3481 atttacagtg ccatcctaga accattacta ttaattaaca ttactgaaaa catcactgca
     3541 gcaaataatg gacaagccat tccacacatc atcaacctat cacaaataca aacattcgct
     3601 ttaatgggtg gtagtggatc tacattatgt ttattaatag caacattctt agtgagtcgc
     3661 aatgctgtct ctaaaaacgt ggctaaatta tcttttggac ctggtatctt caatatcaat
     3721 gaaccagtat tattcggtta cccaatcgtt tataacattt cattagctat tccatttatc
     3781 acagttccag tccttggtat tttaatcagc tacttagcaa cagttacagg attcatgagt
     3841 cctgcattta tacaagttcc ttggactaca ccagtattct taaatgcatg gttagcaaca
     3901 gcaggggacg tgagagcagt tctagttcaa ttcatcatct ttgcacttgg agttcttcta
     3961 tacattccat ttatcaaagt taatgacaaa gttgttgaac aagaaatgga aggttaagat
     4021 tatgaaacaa attttattag tatgtaatgc gggaatgtcg acaagtatgc ttgttaaaaa
     4081 aatgcaacaa agtgcgactg aacgtgggat tgaaatctct atccaagcaa aatcaatgac
     4141 agaagctaaa aagaatatcc atgaagcaga tgtcattcta attgggccac aaattcgtta
     4201 tgagttactt gctgtgaaag aaattgcagg aaatatccct gtagatacca ttgatatgcg
     4261 ggattacgga atgatgaatg gagcaaaagt tctggaacaa gcacttgcat ggataggaga
     4321 aataagatga cagaagaaat ggaatatatc tgttttcagc tcattgccaa tagtggagca
     4381 gccaaatcat cctttattga agccatccaa ctagctaagg caggaaattt aaaagaagcg
     4441 aaaattaaag ttgaagaagc ggaagactcc ttagtagaag cgcacaagat tcactctaat
     4501 ctcatacaaa aagaagctac tggagaaaaa ataggattct ccttgctatt tatgcatgca
     4561 gaagaccaaa tggcttcaac agagattatt cagttgctat ctaaagaatt catcgatctc
     4621 tatcaaaata aataggaaca ctgtcattcc catctgtctt tattaatgac gactacattg
     4681 taggaacctc caaaagttgg acaaaatgat tgaaaggatt ggatcctgca ttacacaaga
     4741 tccaatcctt ttcttttact ctagttttta taatatgttg aatctagaat agtccactgt
     4801 agtttctaaa aaattgctgg aaatggatgt taagctccaa tttatttgtt tatatcttat
     4861 ttcagtttac tatactagct tattattcta gtgaaaaatt gaatgagcca ctatttattt
     4921 aatatgtatg atgagggaat gactcaaaaa cttcttgtcg atataagaat atctgagata
     4981 gtattttaaa acgaaaggac tataaaagct catttccttc gcacttattt tatagaaatc
     5041 tatcattata ctctttagaa atcaaaaagc aacacacctt gtcatcgttt tatcgtaaat
     5101 ctgttaatga gggggctagt cttatctgca atctcaaatc agtgttttga gctgaatggc
     5161 actcgttttc tagtttgctc tttgatttta attgggcctt agtaaatact actcttaaat
     5221 taaactatag tgaattgaaa taagatgtga acaactctat cag
//
LOCUS       DAVKQL010000093         5135 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVKQL010000093 DAVKQL010000000
VERSION     DAVKQL010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5135
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.093"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            258..1316
                     /locus_tag="VW561_002000"
     CDS             258..1316
                     /locus_tag="VW561_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944203.1"
                     /translation="MKDDQKYLLAGLYSLLVAIFYFPLIDSKGIFVSILMAVLLLYLI
                     YFIGTVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREISLLLATIIVYFFTHRILPLSVFTFIFSYILLFAQ
                     SYLGGNTAWIGNRRLIIDDEFEKILLSKSYIKEISSARYSEYLTCKYKNFTPIILLAI
                     FENLLDSYLLQNQSEVDLDIFYKVLPLLYKEKYTMGFNYFVSLNYLLYKVGFLGIIYD
                     NEALRDLSKQYLNKNISELQDGSFEGGIQDAVASKQIVVINEFIACLNSRCVPSQYDR
                     FFYKDRPYIFSRKSPIKG"
     gene            1631..2608
                     /locus_tag="VW561_002001"
     CDS             1631..2608
                     /locus_tag="VW561_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944204.1"
                     /translation="MKNKRYFFDTILIILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     PGIAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYDKPLLLPLISFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKKNCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKINHPISLYGMLDVIKLNLYLRHYNEKNKYESMLNKIL
                     EVRPDFVLIEQNIDDSLNSSQPLSLSLAISEIQLLLEVYMGIKHVSIRR"
     gene            2985..3359
                     /locus_tag="VW561_002002"
     CDS             2985..3359
                     /locus_tag="VW561_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HEU9944205.1"
                     /translation="MELVLPNNYVVLEQEEMMYLDGGFSIPRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGN
                     AITTIWANNDVNRRDPNLNALW"
     gene            3567..4469
                     /locus_tag="VW561_002003"
     CDS             3567..4469
                     /locus_tag="VW561_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944206.1"
                     /translation="MIRKLIIFLLMLPLFVLWLELHLLVSNLQLNLEIPFDFVVSTSL
                     TFFVLILSKIVLDILYALKDLYKKEALITIFPFIFIGRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQN
                     KWQKEDSFESDLTKTLKDKIQKSELSYSDLMSLLLYDAMNQSTFLTDSELFEDILKKI
                     EDSHNTLLCTGLVELLLYEISISKNHNWSEKIDKIRIELIKINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            4525..4878
                     /locus_tag="VW561_002004"
     CDS             4525..4878
                     /locus_tag="VW561_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU9944207.1"
                     /translation="MELVLPNNYVVIDEEVMMYLDGGAYLSKRACKGICAALAMSSGT
                     FIALAGAAVLTKKLINYIKFGGLGGWLIGAAAGVLATAAEKIAYCIGYGVLNRGCDIN
                     GNPYPWDGFISATVR"
     gene            4875..>5135
                     /locus_tag="VW561_002005"
     CDS             4875..>5135
                     /locus_tag="VW561_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944208.1"
                     /translation="MSNVDKIRKIHIIVCWVYIFLSFRVIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLISFPILINKFFPESFLTYT"
BASE COUNT         1701 a          661 c          897 g         1876 t
ORIGIN      
        1 taataaattc tattattata gctcttggta tgatattggc attaattttg gtattatcaa
       61 gagtgtatag aaaatatttt tatagagacg atgatgacta aagtttttat caataatttg
      121 ggctccttgt caactgtagt gggttgaaga aaagctaacg tctggggaag ggtttaaagc
      181 tttgctttat caaatattga cttacagagt tgctctattg tattgatgta ttcttagtaa
      241 ataatatggt gttttgaatg aaagatgatc agaaatattt attagcggga ctttattcgc
      301 ttctagttgc aatattttat tttccattga ttgattccaa ggggattttt gtaagtatat
      361 tgatggcggt tttactttta tatttaatat acttcattgg aacagtcatt catattgtga
      421 taattaagtt tataagaaaa aaatctttta agtatttggt tctatatcca tttacttatg
      481 atgggagctg gagatttcaa cctataaact tactctactt tccagaaatg gtgagagatg
      541 tcattccgat taatttagtt caagaatatt gccaaggaca accttacgga ctgttaaaaa
      601 agatgctgaa aagaattcga ttgtcacgag agatatcttt actcctggct accattatcg
      661 tctatttttt tacgcataga attctacctc tttcagtatt tacgttcata ttttcttaca
      721 tattgctatt tgcacaatct tatttgggtg gtaataccgc ttggattgga aatagaagat
      781 taataataga tgatgaattt gaaaaaatct tgctttcaaa aagttatata aaagaaatat
      841 catctgcacg atactcagaa tacttgacat gtaaatacaa aaactttaca ccaatcatat
      901 tgttagctat ttttgaaaat ttgcttgact cctacttgct tcagaatcag agtgaggttg
      961 acttagatat tttttataaa gttttacctt tgctttataa agaaaaatat actatgggat
     1021 tcaattactt tgtaagttta aattatttac tatataaggt tggatttttg ggaatcattt
     1081 acgataatga agcattaaga gatttgtcaa aacaatattt aaacaaaaat atttcggaat
     1141 tacaagatgg ttctttcgag ggcggtatac aagatgctgt tgcctctaaa caaatagtag
     1201 ttataaatga atttatagct tgtttaaatt ccagatgcgt gccatcccaa tatgatagat
     1261 ttttttataa agatagacct tatatttttt ccagaaagag tcctattaag gggtgaaaat
     1321 ttgttacatt tggattaaca agtgtcctag ggattagaaa taactctagt atagctagat
     1381 atttttgagt aactagaaat atcattagtc tacccatatg attgccagaa aagatgactg
     1441 atgaatctta tttatactgt tgcagtaggc gttgtctagg ataaataagc tatcttgatg
     1501 ggggagattg gaataatttt agtagaaaca ttaaaggatt atggacaagt agcgtaggat
     1561 taggtgatgc aatgtgctaa gttggcttgg tgttagcagt agtattattt aacaatggaa
     1621 agtagactaa atgaaaaata agcgttattt ttttgacaca attttaataa ttttactgct
     1681 tataagcacg atattttgtg tcagcccagt atttataaaa ttggacatct taggaactcc
     1741 ctctcatgcc atcttaacct ttgtattggc gataccgtta ttctatattt tgtctcaatg
     1801 cttacatact ttgctattac tagtttcctc aatcttttgt aaattaagac caatttattt
     1861 ttattttata tttgtgatca ttataggagc taggaaatac tatcgaattc tctttcacca
     1921 actaatggga tttagtccag gaatagcagt gttttataag gagagtcaaa caactaagaa
     1981 tctgtttaaa ttttactact tcttgtactt tacaacacta atttcatact attttttctt
     2041 tacttttgtg tatgacaaac cgttgctttt gcctcttatt tcattttcta ttatcatagc
     2101 attagtacaa aaactttata gaattgagaa tcaacaatta tttctgttaa aatcaaaggt
     2161 actaacaata ctggaatcta agaagaattg tgagtttaat cttcaggatt atcatgaaat
     2221 ttggaaattg caatcaaaat ctgagttgcc atgcgtggct ttatcttata tcagtttaat
     2281 caagccatat ctttcagaga gtgtgcgtga acaaattgat ttattagaag ttaagcgatt
     2341 taaaaaaata aatcatccaa tatcacttta tggtatgttg gatgttataa aattaaatct
     2401 atatttacgg cattataatg aaaaaaataa atatgaatca atgttaaata aaattttaga
     2461 ggtaagacca gactttgttt taatcgagca gaatattgat gatagtttaa atagctcgca
     2521 accattatct ttatctttag ccatttcaga aatccaactt ttgcttgaag tgtatatggg
     2581 gatcaaacat gtttcaatta ggagataaaa ccagaataat atttgccatt ataggggcaa
     2641 gttgcatatt agtcagtatg tatcttatac ctctcattaa cacggataat aaattttggg
     2701 aaatactttt atttatatta ggtgtattgc taattggaat tgcgttacta agtcaaaaaa
     2761 aataaatttt gaaatagtat tagtgagttc gcaatcattt attgctagtg gtttagaact
     2821 gttaagtttc aaacaataat tgattttaaa ccttaggtaa cctataaaaa caaataaggt
     2881 agagaaagga tatcttatct aggtagctaa gtgataaaaa cacagtcgtc tatcacttgt
     2941 taggaaacta tcctaactat gcttaataaa aaaggagaaa ataaatggaa ctcgtattac
     3001 caaataatta tgttgttctt gagcaagaag agatgatgta tcttgatggg ggattttcta
     3061 ttccgagatg gcctgttgca acagccatta atatagcttt taatggtgtt ttaggtggag
     3121 gagcaatcag tctagttaga aattatattc gtaattatgg tttgcggcga gttacaagcg
     3181 caattgctgg agcagctgca agatatgttg gggtacgagt tgcaaataga gtggcaggat
     3241 ttgcactgtc tgctattaat ggatttgcag cttggatgtc aattggcaat gctattacaa
     3301 caatctgggc caataatgat gtaaatagga gagacccaaa tttaaacgcc ttgtggtaac
     3361 tagatatggg tattaaaatg gaaaaaattt ttgttattat tttttttgta tgtctattta
     3421 tatcatccat aactttttta gcctatgatt ttgttagcga agaaataaaa aagttgatta
     3481 tttggattaa tgtcgttttt ttgattttaa tcatagcaat gataatttat ccaaaattaa
     3541 gaaaatgata aggacactgt aaaattatga tacgtaaact aatcattttt ttattaatgc
     3601 tccctctttt tgtcttgtgg ctagagttgc atttgttagt gagtaacttg caattgaatc
     3661 tagaaattcc ctttgatttt gtggttagta cgagtcttac tttttttgtt ttgatattat
     3721 caaaaatagt tttagacata ctatacgcat tgaaagattt gtacaagaaa gaagctctta
     3781 ttacgatttt tccttttatt tttataggca ggaagaaagt aaatgtgagg ttctcgccct
     3841 atttttcatt ccatcgtaaa agtttgtcgc ctgatgattt aagatctagg attatatgga
     3901 gttttattct tgaaattgct attatcctag tatttatttt aaaaattcct tttgcaatca
     3961 tcatgcttac tacaatattt ttctggacta taatggatat caatcatctc gtttttaata
     4021 agacggaatt tctttttaat caaaacaaat ggcaaaaaga agattcgttt gaaagtgatt
     4081 tgacgaaaac gttaaaggat aagatacaaa aatcagaatt aagctattct gacttaatgt
     4141 cccttcttct ctatgatgcg atgaaccaat ccaccttttt aacggatagt gaactttttg
     4201 aggatatttt gaaaaaaatc gaagattctc ataacaccct tctgtgtaca ggtcttgtcg
     4261 agttactgct gtatgaaatt tctattagta agaatcataa ttggtcagaa aagattgata
     4321 aaattaggat agagcttatc aagattaatc aattagattt tttctattat acaagttggt
     4381 taaggcaaaa ttttgatttc tgtatgaata gagagtatca taaaatgaaa tctcggaaat
     4441 tattactgag taataaaaag atagtttaag ttcgtcctat ttgtaaaaca aatgaaacaa
     4501 tttcaactaa ggaggagaaa acaaatggaa cttgtattac caaataatta tgttgtgatt
     4561 gatgaagaag taatgatgta tcttgatggg ggagcttatt taagcaagcg tgcttgtaaa
     4621 ggaatttgcg cagctttagc tatgagttca ggaactttta tagcattagc tggagctgca
     4681 gttttaacca aaaaactaat aaactatatt aagtttggag gtcttggagg ctggcttatt
     4741 ggtgcagcag caggtgtatt ggctacagca gcagaaaaaa tagcttactg tattggatat
     4801 ggtgttctta atagaggttg tgatattaac gggaaccctt atccttggga tggattcata
     4861 tctgcaacag taagatgagt aatgtagata agattcgaaa aatacacatc atcgtgtgtt
     4921 gggtgtatat atttttatca ttcagggtga ttataaatga tacggagtat tttttgctta
     4981 tttttttagc ttttatctac tcaatagtat cactacctct atattctgtg aaaaataaaa
     5041 tagtatctat ctgtctagcc ataaattcga ttctgttaat cagtttcccc attttaatca
     5101 ataagttttt cccagaaagt tttttgactt atacc
//
LOCUS       DAVKQL010000094         4502 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVKQL010000094 DAVKQL010000000
VERSION     DAVKQL010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.094"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            70..>4502
                     /locus_tag="VW561_002006"
     CDS             70..>4502
                     /locus_tag="VW561_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU9944209.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSNSEDALNISDKEKV
                     AENKEKHEDIHSAMETSQDTEEKKTTIIEEKKVVSKNPVIDTKTSNEEAKIKEENSNK
                     SQGDHTDSFVNKNTENPKKEDKVVYIAEFKDKESGAKAIKELSSLKNTKVLYTYDRIF
                     NGSAIETTPDNLDKIKQIEGITSVERAQKVQPMMNHARKEIGVEEAIDYLKSINAPFG
                     KNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKIPHAF
                     NYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQIFSY
                     KMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRALRKAG
                     IPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQTVEFD
                     KVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDLRGKI
                     AVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGTKSQV
                     FSISGDDGVKLWNMINPNKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVGDEKE
                     IDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVINGKST
                     YGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQNTAR
                     PMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGSISLK
                     EIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGKQIVP
                     EIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESVEEME
                     ALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGKPKIP
                     GTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSSGSKIA
                     NIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVISREHF
                     IRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATKIRGQ
                     FEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLITKDTY
                     HKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYAGEGQ
                     GRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANQADEKGMISY
                     YLVDPDQDSSKYQKLGEIAESKFKNLGNGKEGSLKKDTTGVEHHHQENEESIKEKSSF
                     TIDRNISTIRDFENKDLKKLIKKKYKEEDDFVTGGKRTVELDYKYDDKGNITAYEDES
                     ALEYETEKFDEIKSKIYGVLSPSKDGHFEILGKISNVSKNA"
BASE COUNT         1779 a          643 c          882 g         1198 t
ORIGIN      
        1 tataacaaag gctagcaaag ttaaagttta ttatctattg gaagttaaat aaatatgtaa
       61 ggaattaaaa tgaaaaaaag tacagtattg tcattaacca cagctgcagt tattttagca
      121 gcctatgccc ctaatgaggt agtcttagca gacacatcta actctgaaga tgctttaaac
      181 atctctgata aagaaaaagt agcagaaaat aaagagaaac atgaagatat ccatagtgct
      241 atggaaactt cacaggatac tgaagaaaag aaaacaacaa ttattgagga aaaaaaagtt
      301 gttagtaaaa atcctgtgat agacactaaa actagcaatg aagaagcaaa aatcaaagaa
      361 gaaaattcca ataaatccca aggagatcat acggactcat ttgtgaataa aaacacagaa
      421 aatcccaaaa aagaagataa agttgtctat attgctgaat ttaaagataa agaatctgga
      481 gcaaaagcaa tcaaggaact atccagtctt aagaatacaa aagttttata tacatatgat
      541 agaattttta acggtagtgc catagaaaca actccagata acttggacaa aattaaacaa
      601 atagaaggta ttacatcggt tgaaagggca caaaaagtcc aacccatgat gaatcatgcc
      661 agaaaggaaa ttggagttga ggaagctatt gattacctaa agtctatcaa tgctccgttt
      721 gggaaaaatt ttgatggtag aggtatggtc atttcaaata tcgatactgg aacagattat
      781 agacataagg ctatgagaat cgatgatgat gccaaagcct caatgagatt taaaaaagaa
      841 gacttaaaag gaactgataa aaattattgg ttgagtgata aaatccctca tgcgttcaat
      901 tattataatg gtggcaaaat cactgtagaa aaatatgatg atggaaggga ttattttgac
      961 ccacatggga tgcatattgc agggattctt gctggaaatg atactgaaca agacatcaaa
     1021 aactttaacg gcatagatgg aattgcacct aatgcacaaa ttttctctta caaaatgtat
     1081 tctgacgcag gatctgggtt tgcgggtgat gaaacaatgt ttcatgctat tgaagattct
     1141 atcaaacaca acgttgatgt tgtttcggta tcatctggtt ttacaggaac aggtcttgta
     1201 ggtgagaaat attggcaagc tattagggca ttaagaaaag caggcattcc aatggttgtc
     1261 gctacgggta actatgcgac ttctgcttca agttcttcat gggatttagt agcaaataat
     1321 catctgaaaa tgaccgacac tggaaatgta acacgaactg cagcacatga ggatgcgata
     1381 gcggtcgctt ctgctaaaaa tcaaacagtt gagtttgata aagttaacat aggtggagaa
     1441 agttttaaat acagaaatat aggggccttt ttcgataaga ataaaatcac aacaaatgaa
     1501 gatggaacaa aagctcctag taaattaaaa tttgtatata taggcaaggg gcaagaccaa
     1561 gatttgatag gtttggatct taggggcaaa attgcagtaa tggatagaat ttatacaaag
     1621 gatttaaaaa atgcttttaa aaaagctatg gataagggtg cacgcgccat tatggttgta
     1681 aatactgtaa attactacaa tagagataat tggacagagc ttccagctat gggatatgaa
     1741 gcggatgaag gtactaaaag tcaagtgttt tcaatttcag gagatgatgg tgtaaagcta
     1801 tggaacatga ttaatcctaa taaaaaaact gaagtcaaaa gaaataataa agaagatttt
     1861 aaagataaat tggagcaata ctatccaatt gatatggaaa gttttaattc caacaaaccg
     1921 aatgtaggtg acgaaaaaga gattgacttt aagtttgcac ctgacacaga caaagaactc
     1981 tataaagaag atatcatcgt tccagcagga tctacatctt gggggccaag aatagattta
     2041 cttttaaaac ccgatgtttc agcacctggt aaaaatatta aatccacgct taatgttatt
     2101 aatggcaaat caacttatgg ctatatgtca ggaactagta tggcgactcc aatcgtggca
     2161 gcttctactg ttttgattag accgaaatta aaggaaatgc ttgaaagacc tgtattgaaa
     2221 aatcttaagg gagatgacaa aatagatctt acaagtctta caaaaattgc cctacaaaat
     2281 actgcgcgac ctatgatgga tgcaacttct tggaaagaaa aaagtcaata ctttgcatca
     2341 cctagacaac agggagcagg cctaattaat gtggccaatg ctttgagaaa tgaagttgta
     2401 gcaactttca aaaacactga ttctaaaggt ttggtaaact catatggttc catttctctt
     2461 aaagaaataa aaggtgataa aaaatacttt acaatcaagc ttcacaatac atcaaacaga
     2521 cctttgactt ttaaagtttc agcatcagcg ataactacag attctttaac tgacagatta
     2581 aaacttgatg aaacatataa agatgaaaaa tctccagatg gtaagcaaat tgttccagaa
     2641 attcacccag aaaaagtcaa aggagcaaat atcacatttg agcatgatac tttcactata
     2701 ggcgcaaatt ctagctttga tttgaatgcg gttataaatg ttggagaggc caaaaacaaa
     2761 aataaatttg tagaatcatt tattcatttt gagtcagtgg aagaaatgga agctctaaac
     2821 tccaacggga agaaaataaa cttccaacct tctttgtcga tgcctctaat gggatttgct
     2881 gggaattgga accacgaacc aatccttgat aaatgggctt gggaagaagg gtcaagatca
     2941 aaaacactgg gaggttatga tgatgatggt aaaccgaaaa ttccaggaac cttaaataag
     3001 ggaattggtg gagaacatgg tatagataaa tttaatccag caggagttat acaaaataga
     3061 aaagataaaa atacaacatc cctggatcaa aatccagaat tatttgcttt caataaccaa
     3121 gggatcaacg ctccatcatc aagtggttct aagattgcta acatttatcc tttagattca
     3181 aatggaaatc ctcaagatgc tcaacttgaa agaggattaa caccttctcc acttgtatta
     3241 agaagtgcag aagaaggatt gatttcaata gtaaatacaa ataaagaggg agaaaatcaa
     3301 agagacttaa aagtcatttc gagagaacac tttattagag gaattttaaa ttctaaaagc
     3361 aatgatgcaa agggaatcaa atcatctaaa ctaaaagttt ggggtgactt gaagtgggat
     3421 ggactcatct ataatcctag aggtagagaa gaaaatgcac cagaaagtaa ggataatcaa
     3481 gatcctgcta ctaagataag aggtcaattt gaaccgattg cggaaggtca atatttctat
     3541 aaatttaaat atagattaac taaagattac ccatggcagg tttcctatat tcctgtaaaa
     3601 attgataaca ccgcccctaa gattgtttcg gttgattttt caaatcctga aaaaattaag
     3661 ttgattacaa aggatactta tcataaggta aaagatcagt ataagaatga aacgctattt
     3721 gcgagagatc aaaaagaaca tcctgaaaaa tttgacgaga ttgcgaacga agtttggtat
     3781 gctggcgccg ctcttgttaa tgaagatgga gaggttgaaa aaaatcttga agtaacttac
     3841 gcaggtgagg gtcaaggaag aaatagaaaa cttgacaaag acggaaatac catttatgaa
     3901 attaaaggtg cgggagattt aaggggaaaa atcattgaag tcattgcatt agatggttct
     3961 agcaatttca caaagattca tagaattaaa tttgctaatc aggctgatga aaaggggatg
     4021 atttcctatt atctagtaga tcctgatcaa gattcatcta aatatcaaaa gcttggcgag
     4081 attgcagaat ctaaatttaa aaatttagga aatggaaaag agggtagtct aaaaaaagat
     4141 acaactgggg tagaacatca tcatcaagaa aatgaagagt ctattaaaga aaaatctagt
     4201 tttactattg atagaaatat ttcaacaatt agagactttg aaaataaaga cctaaagaaa
     4261 ctcattaaaa agaaatataa ggaagaagac gactttgtaa ctggtggaaa aagaacagta
     4321 gaacttgatt ataaatatga cgataaagga aatataactg cctatgaaga tgaaagtgct
     4381 ctagaatatg aaactgaaaa atttgacgaa ataaaatcaa aaatttatgg tgttctaagt
     4441 ccatctaaag atggacactt tgaaattctt ggaaagataa gcaatgtttc taaaaatgcc
     4501 aa
//
LOCUS       DAVKQL010000095         4362 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVKQL010000095 DAVKQL010000000
VERSION     DAVKQL010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.095"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            713..1705
                     /locus_tag="VW561_002007"
     CDS             713..1705
                     /locus_tag="VW561_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9944210.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPNSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1707..2525
                     /locus_tag="VW561_002008"
     CDS             1707..2525
                     /locus_tag="VW561_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEU9944211.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVIYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2527..3312
                     /locus_tag="VW561_002009"
     CDS             2527..3312
                     /locus_tag="VW561_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9944212.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3351..3629
                     /locus_tag="VW561_002010"
     CDS             3351..3629
                     /locus_tag="VW561_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEU9944213.1"
                     /translation="MFVIEEVKDENQKKAVVAEVLKDLPEWFGIPESTQAYIEGTTTL
                     QVWTAYQESDLTRFVSLSYSSEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            3614..3811
                     /locus_tag="VW561_002011"
     CDS             3614..3811
                     /locus_tag="VW561_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9944214.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFPQLWNPQNPCQILIKKLE"
     gene            complement(4192..4278)
                     /locus_tag="VW561_002012"
                     /pseudo
     CDS             complement(4192..4278)
                     /locus_tag="VW561_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
BASE COUNT         1216 a          747 c          928 g         1471 t
ORIGIN      
        1 attatggtat cgaatcttca tcagaatgat aaaattaatc aattgatatc tgattacaaa
       61 cagaatatga aagcttttta tatcactatt gaaaaattta tacgagatga tgaaagcctt
      121 aagtgttatt ttataaaggt tatttcaagt cgttccaagg taacaagtct agatcagatt
      181 gaagctgata aaacgataca aagaaaatat tcaagtgagc taaaaaaatt tattggattt
      241 tataatgaga ttatttgtga ggaaaatagt ttcctacatg tacgaaagag gtggtcgagt
      301 tggtttaggt agtcgatgcg tgagttgata attctcaggg tatggacttc tttttcatga
      361 atgaggtaaa agagcaggta ttgtttagag acaatcattc tgagcatatt ttctggatag
      421 agggagtatc cgattttatg atcaaagtta ataccgccct ctggtgagaa gatgagtagg
      481 ttggtaattt aaactattaa acagaatttt tgattaaaag tattatttta tgagagaaat
      541 cctaatttca caatccatag gcaaacgctt gcatttcgtt ttttattgga ctataatagg
      601 ttggtataaa gccttttgta gtaataaaat gtagaaggtg tagaaagcaa ggatttagaa
      661 tatttgtagt taaaaacaca atgttgctat tccttacgat agggagatag atatggcaat
      721 gatagaagtg gaacatcttc agaaaaattt tgtgaagact gttaaggaac cgggcttgaa
      781 gggggctttg cgctccttta ttcatcctga aaagcagacc tttgaagcgg tcaaggattt
      841 gacctttgag gttccaaaag ggcagatttt aggatttatc ggggcaaatg gtgctgggaa
      901 gtcgacaacc attaaaatgc tgacaggaat tttgaaacca acatctggtt tttgtcggat
      961 taacggcaag attccccagg acaatcggca agattatgtc aaagatattg gcgtagtctt
     1021 tggacaacgc acccagctat ggtgggattt ggctctgcaa gagacctaca ctgtcttaaa
     1081 agagatttat gatgtgccaa actcgctctt tcataagcgc atggactttt tgaatgaagt
     1141 cttggatttg aaggacttta tcaaggatcc cgtgcggact ctttcactgg gacaacggat
     1201 gcgggcggat attgcggcct ccttgctcca caatcccaag gttctttttt tagatgagcc
     1261 gaccattggt ttggacgttt cggttaagga taatattcgt cgggcaatta ctcagatcaa
     1321 tcaagaggaa gaaactacca ttcttttgac cactcacgat ttgagtgata ttgagcaact
     1381 ttgtgatcgg attttcatga ttgacaaggg gcaagagatt tttgatggaa cggtgagcca
     1441 actcaaggag acctttggta agatgaagac tctctctttt gaactgctac caggtcaaag
     1501 tcatctcgtc tctcactatg aaggtctgtc tgatatgacc attgatagac aaggaaacag
     1561 cctcaacatt gaatttgata gttctcgcta ccagtcagct gacattatca agcaaaccct
     1621 gtctgatttt gaaatccgcg atttgaagat ggtggatacg gatattgagg atattatccg
     1681 tcgcttctac cgaaaggagc tctaggatga tcaaattgtg gagacgttat aaacccttta
     1741 tcaatgcagg ggttcaggag ttgattactt accgagtcaa ctttattctc tatcggattg
     1801 gcgatgtcat gggggctttt gtggcctttt atctctggaa ggctgtcttt gattcttcgc
     1861 aagagtcttt gattcagggc ttcagtatgg cggatatcac cctctacatc atcatgagtt
     1921 ttgtgaccaa tcttctgact agatccgatt cgtcctttat gattggggag gaggtcaagg
     1981 atggctccat tatcatgcgt ttgttgcgac cagtgcattt tgcggcctcc tatcttttca
     2041 ccgagcttgg ttccaagtgg ttgattttta tcagcgttgg ccttccattt ttaagtgtca
     2101 ttgtcttgat gaaaatcata tcgggtcaag gtattgtaga ggtgctagga ttaactgtca
     2161 tttatctttt tagcttaacg ctcgcctatc tgattaactt tttctttaat atttgctttg
     2221 gattttcagc ctttgtgttt aaaaatcttt ggggttccaa cctacttaag acttccatag
     2281 tggcttttat gtcggggagt ttgattccct tggcattctt tccaaaggtt gtttcagata
     2341 ttctctcctt tttgcctttt tcatccttga tttatactcc agttatgatc attgttggaa
     2401 aatacgatgc cagtcagatt cttcaggcac tccttttgca gttcttctgg ctcttagtga
     2461 tggtgggatt gtctcagttg atttggaaac gggtccagtc ctttatcacc attcaaggag
     2521 gttagtatga aaaaatatca acgaatgcat ctgattttta tcagacaata catcaaacaa
     2581 atcatggaat ataaggtgga ttttgtggtt ggtgtcttgg gagtctttct ggctcaaggc
     2641 ttgaatctct tgtttctcaa tgtcatcttt caacatattc cattcctaga aggctggacc
     2701 tttcaagaga tagctttcat ttatggattt tccttgattc ccaagggaat ggaccatctc
     2761 ttttttgaca atctctgggc actagggcaa cgcctagtcc gaaaagggga gtttgacaag
     2821 tatctgactc gtcccatcaa tcctctcttt catatcctag ttgaaacctt tcagattgat
     2881 gccttgggtg aactcttagt cggtggtatt ttattgggaa caacagtgac cagcattgtt
     2941 tggactcttc caaaattcct gcttttccta gtttgtattc cttttgcgac cttgatttat
     3001 acttctctta aaatcgcaac agccagtatc gccttttgga ctaagcagtc aggcgccatg
     3061 atttacatct tctatatgtt caatgacttt gctaagtatc cgatttctat ttacaattct
     3121 cttcttcgtt ggttgattag ctttatcgtg cctttcgcct ttacagccta ctatccagct
     3181 agctatttct tacaggaaaa ggatgtgttc tttaacgtag gaggtttgat gttgatttct
     3241 ctggttttct ttgttatttc ccttaaactt tgggataagg gcttagattc ctacgaaagt
     3301 gcgggttcgt aaaagctaaa gtaagactaa aatcaagaaa ggaacttatg atgtttgtaa
     3361 ttgaagaagt caaggatgaa aatcaaaaaa aggcagttgt agctgaggtt ttgaaggatt
     3421 tgccagaatg gtttggaatc ccagaaagca cacaagccta tatagaagga accacgacac
     3481 tgcaagtttg gaccgcctat caggagagtg atttgactag atttgtaagc ttatcctatt
     3541 cgagtgaaga ttgtgcagag attgattgtc tcggcgtaaa aaagcttatc aaggtagaaa
     3601 aattgggagc caattgcttg ctactttaga gagtgaagct cgtaaaaaag ttggttatct
     3661 gcaggtcaaa acagtggcag aaggttctaa taaagattat gatcgaacaa atgactttta
     3721 tcgaggtctt ggctttaaaa agttagagat ttttcctcaa ctatggaatc cgcaaaatcc
     3781 ttgtcagatt ttgattaaaa agcttgaata atattacttg acatctattc tcagagtgct
     3841 atactgtaag tgtaatcgcc gatttagctc agttggtaga gcaaggcact cgtaaagcct
     3901 aggttatagg tagataaacg actgaggatt tgaaaaaata gataggtaga agataaccgt
     3961 taagccttat tcttagcggt tatttatatt gtttaatagc gctgatattt catcaattat
     4021 gcctgttttc gtgtttctgg tagttgttca agtttattgc tactattttt gatggtatga
     4081 atgtgcttat aatgtatccc ggttaacgaa agttttggac ttatactctt agaaaatctc
     4141 ttcaaaccac gtcaacgtcg ccttgccgtg cgtatggtta ctgactacgt cagttctatc
     4201 cacaacctca aaaccgtgtt ttgagcaacc tgcgactagt ttcctagttt gctctttgat
     4261 tttcattgag tactaatctt attttttaac gtgtgctaaa aaataaatta atgcatgtta
     4321 atttttcttg aattaaattt ctttaaatga tatactgttt tt
//
LOCUS       DAVKQL010000096         4333 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVKQL010000096 DAVKQL010000000
VERSION     DAVKQL010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.096"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            67..1056
                     /gene="manX"
                     /locus_tag="VW561_002013"
     CDS             67..1056
                     /gene="manX"
                     /locus_tag="VW561_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEU9944215.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            1084..1887
                     /locus_tag="VW561_002014"
     CDS             1084..1887
                     /locus_tag="VW561_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEU9944216.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            1911..2822
                     /locus_tag="VW561_002015"
     CDS             1911..2822
                     /locus_tag="VW561_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9944217.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(2878..4212)
                     /gene="pepC"
                     /locus_tag="VW561_002016"
     CDS             complement(2878..4212)
                     /gene="pepC"
                     /locus_tag="VW561_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEU9944218.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALECRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
BASE COUNT         1180 a          932 c          917 g         1304 t
ORIGIN      
        1 ttatacttaa acagtcttaa agttttctta aatgaaaact aaaacaaaaa aggaggaatg
       61 acaataatga gtatcggaat cattattgcg agccacggcg aatttgctgc gggtattcat
      121 cagtcaggat ctatgatctt tggtgaacaa gaaaaggttc aagttgtgac ctttatgcca
      181 aatgaaggtc ctgatgatct atacgctaag tttaataacg ctgttgctgc atttgacgca
      241 gaagatgagg ttctagtttt ggctgacctt tggagtggtt ctccatttaa ccaagctagt
      301 cgcgtgatgg gagaaaatcc tgagcgtaag tttgccatca tcacaggact taacttaccg
      361 atgttgattc aagcctacac agagcgcctc atggacgctg ctgcaggtgt agaaaaagtc
      421 gctgctaata tcattaaaga agccaaagat ggcatcaaag ctcttccaga agagctaaat
      481 ccagttgaag aagttgcaag cgctgcagct gctccagttg cccaaactgc tatcccagaa
      541 ggaactgtta tcggagacgg taaattgaaa atcaatcttg cccgtcttga cacacgtcta
      601 cttcacggtc aggttgcaac tgcttggact ccagattcaa aagcaaatcg tatcatcgtt
      661 gcttcagata acgtggctaa agacgacctt cgtaaagaat tgattaaaca agcagctcca
      721 ggtaatgtca aggctaacgt ggttccaatt caaaaactga ttgagatttc aaaagaccca
      781 cgttttggag aaacacatgc ccttatcttg tttgaaacac ctcaagatgc ccttcgtgcc
      841 atcgaaggcg gcgtgccaat caagactctt aacgttggtt ctatggctca ctcaacaggt
      901 aaaacattgg tcaataccgt tttgtctatg gacaaagaag atgttgctac atttgaaaaa
      961 atgcgtgact tgggtgttga atttgatgtc cgtaaagtac caaatgattc taaaaaagat
     1021 ttgtttgact tgattaacaa agccaatgtc aaataagcca ttatttatga aaggatttta
     1081 aacatgtcta ttatttctat ggttttagta gtcgttgtag ccttctttgc aggtcttgaa
     1141 ggcatcctcg accagttcca atttcaccaa ccacttgtag cctgtaccct tattgggctt
     1201 gtaacaggtc acttggaagc agggattatc ctcggtggat cgcttcaaat gattgccctt
     1261 ggttggtcaa atatcggtgc tgctatcgct cctgatgctg cacttgcttc tgtcgctgct
     1321 gccattatca tggttcttgg tggtgacttt accaagactg gtatcggtgt tgcccaagcg
     1381 gttgctatcc ctcttgccgt agctggactt ttcttgacaa tgattgttcg tacaatttca
     1441 gttggtttgg ttcatactgc agatgctgcc gctaaaaaag gtgacttcgg cgctgtggag
     1501 cgtgcgcatt tcattgcgct acttttccaa ggacttcgta tcgcgcttcc tgcagctctt
     1561 ctccttatgg taccaactga aactgtacaa agtatcctta gtgccatgcc agactggctc
     1621 aaagatggta tggctatcgg tggtggtatg gtcgttgccg ttggttacgc catggttatc
     1681 aacatgatgg caactcgtga agtatggcca ttcttcgctc ttggtttcgt tctcgctgct
     1741 gtgtcagata ttactctaat cggattcggt gctatcggtg ttgctatcgc tcttatctac
     1801 cttcaccttt ctaaaactgg tggaaatggt ggcggaggag ccgcaacttc taacgaccca
     1861 atcggcgata tcctagaaga ctactaagat aagaaaggac tgaaaacatc atgactgaaa
     1921 aacttcaatt aactaaatca gatcgtaaaa aagtttggtg gcgttcaacc ttcttacaag
     1981 ggtcttggaa ctttgaacgg atgcaaaact tgggctgggc ttatacactc attccagcta
     2041 tcaaaaaact ctatactaaa aaagaagatc aaatcgctgc tcttgagcgt caccttgagt
     2101 tcttcaacac tcatccatac gtagctgctc cagtcatggg ggttactctt gcgcttgaag
     2161 aagaacgtgc taacggtgtg gaaatcgatg acgctgctat ccaaggggtt aaaatcggta
     2221 tgatgggacc tcttgctggt atcggtgacc cagtattctg gtttacagta cgcccaatcc
     2281 ttggatctct cggtgcttca cttgccctta ctggcaatat cttggggcca ctcctcttct
     2341 ttgttgcatg gaacttgatt cgtatgtcat tcttgtggta tgttcaagag attggataca
     2401 aggctggatc agaaatcact aaagatatgt ctggtggtat ccttcaagat atcactaaag
     2461 gagcttctat ccttgggatg ttcattcttg ctgtccttgt tcaacgctgg gtaaatatta
     2521 aatttgcttt cgatgtttct aaagttcaac tagatgaaaa ggcttatatc cattgggata
     2581 aattgccaga agggtctaaa ggtatccaag aagcattcgc acaagtagga caaggattgt
     2641 ctcaaactcc tgaaaaagtt actactttcc aacaaaactt ggatatgttg attcctggat
     2701 tatcaggact actccttact ttactttgca tgtacttact taagaaaaaa gtatctccaa
     2761 tcactattat ccttgccctc ttcgcagtgg gtattgtggc acatgttctt cacatcatgt
     2821 aatcaagcaa ctaaaaagga accaggttct aaaatctgat tccttttttc tatgctttta
     2881 ttcagccaag gctcccattg gatcccatgg tgcaagtacg attggttctg ctccataggc
     2941 agcttgttct tctgctgtca gcaattcctt acgaacaacg atttggtatg tgtattcgtc
     3001 catccaagcg tctgaggcaa caaagtaacc atctgtaccg accttgtctc cccatgagtt
     3061 ttcaaccttc cacttggttg atttaccatt ttcgtccaag tcaacacctg tcaagaccat
     3121 ggcgtgggtc atcaagcttt cgctatagtc caaacgtcca gccttgtctt gagtaagttt
     3181 aatgtccatg cttgattcaa agtcataaac atctgtcgca aggatgccag ccttacggtt
     3241 gctgagctgg ccgacatcag aaccaaacca aacagtctca cctgcttgca tttgggcaat
     3301 cgccaattct ttcaagcgct ccattggaac gttgatgtag cgaactgcac ggctaccaac
     3361 cacattcccc aacatctcaa ctgtgtaaga ttttccgtaa ggtttatcag cagttggagc
     3421 attgataaca gaaacgtagt cttctaaagg aagattgaca tatttcttgt aaaactcttg
     3481 tggtgtgatt cctttttcac ttttatagtt gttatcttta tcgcgataag caaagtcaaa
     3541 cttgcgtggt ggaagtccta atgacatagc aagaaagtta aagatttctt gcaagaggtc
     3601 ttctttctta gcttgaacag tcgcttgatc tgcaccagaa acaagcaaat cacgcaagat
     3661 ttgagcatct tgacgaagca atttattaag gatcgcattt agctcacgac tgctgctaga
     3721 tgaaacagac tcaggataaa ctgacttagg cacgacaccg tatttttcaa agagggaaac
     3781 gaccatatcc cattgaccgc catcttgttg aggtgtttgg agtaagaagc taaccttgcg
     3841 gctagtcaat tcttggtctg aagtcgcaat gacttgctcc aagaaccagt ttgatttctc
     3901 atacttatcc cagaagaaag tgtgggcttg tgacaactca aagttctcca atttgtattg
     3961 cgagatgagt ttgtggcgga aggtgttgag agccgcaaac atccagcaac gaccagacgc
     4021 tttctggtta gtgaccttgt ccttggttaa atccaatgag aaaacaggtg tgttgtctac
     4081 atggctttgg cgacattcca gagctgcaaa aattccgttg tggctggcag cattttcaat
     4141 cgcttggtat tttacatttg cttcatagtt ggcaaatagt ttatcagtaa atgattcttg
     4201 aatcgcgttc atagattcct ccttttattc tacagtctat tataactctt ttcacaaagg
     4261 aagcaactaa aaaacataaa aggcgaggac attgtccctc acctttttcg aatttatact
     4321 cttcgaaaat ctc
//
LOCUS       DAVKQL010000097         3729 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVKQL010000097 DAVKQL010000000
VERSION     DAVKQL010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.097"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            148..375
                     /locus_tag="VW561_002017"
     CDS             148..375
                     /locus_tag="VW561_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944219.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            663..3593
                     /gene="nanA"
                     /locus_tag="VW561_002018"
     CDS             663..3593
                     /gene="nanA"
                     /locus_tag="VW561_002018"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HEU9944220.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGGGSSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKISRDYYARDLE
                     NVETVIEKEDVETNAANGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVS
                     SATKKDEYFTMAVYNNTATLEGRGSDGKQFYGNYNDAPLKVKPGQWNSVTFTVEKPTP
                     ELPKGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLT
                     VYNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGKPNKDGIKSYRI
                     PALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKAS
                     DPSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQIL
                     YREGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGNQLLGNIYFTT
                     NKTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGP
                     HKGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTM
                     NNRRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYV
                     QMSAIHTMHNGKEYIILSNAGGPNRENGMVHLARVEENGELTWLKHNPIQKGEFAYNS
                     LQELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKDLISPTEAKVKRTREMGKGEM
                     GKGEMGKGEMGKGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKED
                     IGQEIIGIAKGSIESMHNLPVNLAGARVPGGVNGNKAAVHEVPEFTGGVNGTEPAVHE
                     IAEYKGSDSLVTLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGK
                     KREQ"
BASE COUNT         1270 a          624 c          858 g          977 t
ORIGIN      
        1 tgaggtataa taaatttact aacgcttata aaaagtgata gaatctatct ttatgtatat
       61 ttaaagatag attgctgtaa aaatagtagt agctatgcga aataacagat agagagaagg
      121 gattgaagct tagaaaaggg gaataatatg atatttaagg cattcaagac aaaaaagcag
      181 agaaaaagac aagttgaact acttttgaca gtttttttcg acagttttct gattgattta
      241 tttcttcact tatttgggat tgtccccttt aagctggata agattctgat tgtgagcttg
      301 attatatttc ccattatttc tacaagtatt tatgcttatg aaaagctatt tgaaaaagtg
      361 ttcgataagg attgagcagg aagtatggtg taaatagcat aggctgatgt ccatcatttg
      421 cttataaaga gatattttag tttaattgca gcggtgtcct ggtagataaa ctagattggc
      481 aggagtctga ttggagaaag gagaggggaa atttggcacc aatttgagat agtttgttta
      541 gttcattttt gtcatttaaa tgaactgtag taaaagaaag ttaataaaag acaaactaag
      601 tgcattttct ggagtaaatg tcttatttca gaaatcagga tatagatata gagaggatca
      661 gtatgaatcg gagtgttcaa gaacgtaagt gtcgttatag cattaggaaa ctatcggtag
      721 gagcggtttc tatgattgta ggagcagtgg tatttggaac gtctcctgtt ttagctcaag
      781 aaggggcaag tgagcaacct ctggcaaatg aaactcaact ttcggggggg gggagctcaa
      841 ccctaactga tacagaaaag agccagcctt cttcagagac tgaactttct ggcaataagc
      901 aagaacaaga aaggaaagat aagcaagaag aaaaaatttc aagagattac tatgcacgag
      961 atttggaaaa tgtcgaaaca gtgatagaaa aagaagatgt tgaaaccaat gcggcaaatg
     1021 gtcagagagt tgatttatca agtgaactag ataaactaaa gaaacttgaa aacgcaacag
     1081 ttcacatgga gtttaagcca gatgccaagg ctccagcatt ctataatctc ttttctgtgt
     1141 caagtgctac taaaaaagat gagtacttca ctatggcagt ttacaataat actgctactc
     1201 tagaggggcg tggttcggat gggaaacagt tttacggtaa ttacaacgat gcacccttaa
     1261 aagttaaacc aggtcagtgg aattctgtga ctttcacagt tgaaaaaccg acaccagaac
     1321 tacctaaagg ccgagtgcgc ctctacgtaa acggggtatt atctcgaaca agtctgaaat
     1381 ctggcaattt cattaaagat atgccagatg taacgcatgt acaaatcgga gcaaccaagc
     1441 gtgccaacaa tacggtttgg gggtcaaatc tacagattcg gaatctcact gtgtataatc
     1501 gtgctttaac accagaagag gtacaaaaac gtagtcaact ttttaaacgc tcagatttag
     1561 aaaaaaaact acctgaagga gcggctttaa cagagaaaac ggacatattc gaaagcgggc
     1621 gtaacggtaa accaaataaa gatggaatca agagttatcg tattccagca cttctcaaga
     1681 cagataaagg aactttgatc gcaggtgcag atgaacgccg tctccattcg agtgactggg
     1741 gtgatatcgg tatggtcatc agacgtagtg aagataatgg taaaacttgg ggtgaccgag
     1801 taaccattac caacttacgt gacaatccaa aagcttctga cccatcgatc ggttcaccag
     1861 tgaatatcga tatggtgttg gttcaagatc ctgaaaccaa acgaatcttt tctatctatg
     1921 acatgttccc agaagggaag ggaatctttg gaatgtcttc acaaaaagaa gaagcctaca
     1981 aaaaaatcga tggaaaaacc tatcaaatcc tctatcgtga aggagaaaag ggagcttata
     2041 ccattcgaga aaatggtact gtctatacac cagatggtaa ggcgacagac tatcgcgttg
     2101 ttgtagatcc tgttaaacca gcctatagcg acaagggtga tctatacaag ggtaaccaat
     2161 tactaggcaa tatctacttc acaacaaaca aaacttctcc atttagaatt gccaaggata
     2221 gctatctatg gatgtcctac agtgatgacg acgggaagac atggtcagcg cctcaagata
     2281 ttactccgat ggtcaaagcc gattggatga aattcttggg tgtaggtcct ggaacaggaa
     2341 ttgtacttcg gaatgggcct cacaagggac ggattttgat accagtttat acgactaata
     2401 atgtatctca cttaaatggc tcgcaatctt ctcgtgtcat ctattcagat gatcatggaa
     2461 aaacttggca tgctggagaa gcggtcaacg ataaccgtca ggtagacggt caaaagatcc
     2521 actcttctac gatgaacaat agacgtgcgc aaaatacaga atcaacggtg gtacaactaa
     2581 acaatggaga tgttaaactc tttatgcgtg gtttgactgg agatcttcag gttgctacaa
     2641 gtaaagacgg aggagtgact tgggagaagg atatcaaacg ttatccacag gttaaagatg
     2701 tctatgttca aatgtctgct atccatacga tgcataatgg aaaagaatac attatcctca
     2761 gcaatgcagg tggaccgaat cgtgaaaatg ggatggtcca cttggcacgt gtcgaagaaa
     2821 atggtgagtt gacttggctc aaacacaatc caattcaaaa aggagagttt gcctataatt
     2881 cgctccaaga attaggaaat ggggagtatg gcatcttgta tgaacatact gaaaaaggac
     2941 aaaatgccta taccctatca tttagaaaat ttaattggga atttttgagc aaagatctga
     3001 tttctcctac cgaagcgaaa gtgaagcgaa ctagagagat gggcaaagga gagatgggca
     3061 aaggagagat gggcaaagga gagatgggca aaggagttat tggcttggag ttcgactcag
     3121 aagtattggt caacaaggct ccaacccttc aattggcaaa tggtaaaaca gcgactttcc
     3181 taacccagta tgatagcaag accttgttgt ttgcagtaga taaggaagat atcggacagg
     3241 aaattattgg tatagctaaa ggaagcatcg aaagtatgca taatcttcct gtaaatctag
     3301 caggtgccag agttcctggc ggagtaaatg gtaacaaagc agcggtgcat gaagttccag
     3361 aatttacagg gggagttaat ggtacagagc cagctgttca tgaaatcgca gagtataagg
     3421 gatctgattc gcttgtaact cttactacaa aagaagatta tacttacaaa gctcctcttg
     3481 ctcagcaggc acttcctgaa acagggaaca aggagagtga cctcctagct tcactaggac
     3541 taacagcttt cttccttggt ctgtttacgc tagggaaaaa gagagaacaa taagagaaga
     3601 attctaaaca tttgattttg taaaaataga aggagatagc aggttttcaa gcctgctatc
     3661 tttttttgat gacattcaag ctgatacgaa atcataagag gtctgaaact actttcagag
     3721 tagtctgtt
//
LOCUS       DAVKQL010000098         3649 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVKQL010000098 DAVKQL010000000
VERSION     DAVKQL010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.098"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(<1..132)
                     /locus_tag="VW561_002019"
     CDS             complement(<1..132)
                     /locus_tag="VW561_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU9944221.1"
                     /translation="MKLTYDDKVQIYELRKQGYSLEKLSNKFEINNSNLRYMIKLIDR
                     "
     gene            412..504
                     /locus_tag="VW561_002020"
                     /pseudo
     CDS             412..504
                     /locus_tag="VW561_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(760..1380)
                     /locus_tag="VW561_002021"
     CDS             complement(760..1380)
                     /locus_tag="VW561_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9944222.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLHTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(1380..1862)
                     /locus_tag="VW561_002022"
     CDS             complement(1380..1862)
                     /locus_tag="VW561_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEU9944223.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALGLDQMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(1862..2704)
                     /locus_tag="VW561_002023"
     CDS             complement(1862..2704)
                     /locus_tag="VW561_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEU9944224.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDLKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(2799..3599)
                     /locus_tag="VW561_002024"
     CDS             complement(2799..3599)
                     /locus_tag="VW561_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEU9944225.1"
                     /translation="MRKQIHPLILFSFLGIMMFILIINRPLNDSQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSAQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
BASE COUNT         1132 a          661 c          722 g         1134 t
ORIGIN      
        1 acgatcaatc aatttaatca tgtacctaag attagaattg tttatctcaa atttatttga
       61 aagtttctct aagctatatc cttgttttct aagttcatag atctgaactt tatcatcata
      121 agttaatttc ataataaaaa caccccaaaa gttagatttt ttctgtctaa cttttggggg
      181 gcagttcatt taacacctga taatatgcgt ttttttattt taaagacttt ttgaccagcc
      241 tcattttttt aacttgatac tcagtgaaaa gccaaaatta aactaggaag ctagctgtag
      301 gctgctcaaa gaacagcttt gaggttgcag ataaaacttg tgaggtcacc aacatatata
      361 atgtgaagct gacatggttt gaatagattt tagaagggta tgagtctgga acttttaatg
      421 gataatgcaa gattccatag aatgggtaag ctagagttct tatgtgaaga gtttgggcat
      481 aaacttttac cttttcctcc ctacttatct tagtatagaa aagtgaatct gaaatagtac
      541 ataactgctt ctaaaacatt cttataatat gatttaaatt ctcaaatcat attattcagt
      601 tcttatttca ttttgctcta caatcctgtt gagaagacac gtgttcatat caaaaaggta
      661 ttggcaagtt gcaatacctt tttacaaggt tcttttgtct tatttttgtt tcaactgact
      721 atatctccta tggttctagt tcagaaggct aggctataat tatgattgat aagaagtatc
      781 attccaagta ttgagagtga atgtttcaaa atcatgggtt tctataatgg tcaggctggc
      841 atttgctaga ccgccatctt tacgaagaag tggttcttta tagcctagga gagtacgaag
      901 actggcagta agattggcgc cgtgtccgac aattagaata cgctcagctg gactatcttt
      961 taatgatttg ataaattgga tggtccgttg agttgtacta tagagggatt cggctccgaa
     1021 cattcgagtg tcaaattgag caagatttga acgaaaagcc tggatttgtt gcgggtaaat
     1081 agcttccaag gttgcaattt tcaaaccttc taacttccca agttgccatt cacggagatt
     1141 aggaacgatt tctaaagaac agggggtatg gagttgactt tggataatct cagcagattt
     1201 gaccgctcga ggtaaatcac ttgaataaat ctgatcaaaa ggaatttcct tgagatactg
     1261 accaagtcgt tttagggttt caatggattc aggaagaaga ggagaatcac cgctagcacc
     1321 ttgaaaacga ccttcttggt tccagagggt acgaccgtgg cggacaaagt agagtttcat
     1381 tacttgatgt cctccaaaat atctacaaag tctgccttta caaagctagc caagtcttgt
     1441 ggcgcgacga taatgctgtg tccgacttcg cctgcagaga caatcatttg atccaaacct
     1501 agagcaattt tatcgataaa aatgggataa ttgtgtttct gacgaattcc gacaggatta
     1561 ttggctccat gaatgtaacc agttgttttt tctaagtcct tttgtggaat catgctcact
     1621 tttttattgc cagaaatttt agctagtttc ttttcagaca agtgctgagt gatagggaca
     1681 attccgataa tcggtccggt cttgtctccc aaaagcgcca aggttttgaa aatctgatct
     1741 cgttcataac cttgaggaag ctctccttct agggcattga tttgaatccc ctgatgaggg
     1801 atagctgctt tagataggat ttgttctacc aatgtttttt tgattttaac tttttttgcc
     1861 attatttata tttatcctcc aattgactca tccaaatacc aagccagatt cccagcgcaa
     1921 agaagaaggc gatgatgaca taaccgacaa gtgaaagtcc tgtgtattgg atactttcag
     1981 cgtttcctgc atttggaatt aagatcaaaa gggtacttga taggacgata ccgatgatga
     2041 aatgatagac gcgtgagtgg tagttgttta aggcataatc catcaatttt gaaaaaacga
     2101 tgagagttgc acctgcacca attccaatcg gaaagaaggt tcccaagaga tcaaaagttt
     2161 taaaaccagt caacatagga gcatagagtc ccaaaatcaa aagtaaattc gatgggctga
     2221 ggccaggaac caagacgcca agggccaata gtgcgcctgc taggacgaag ttaagaaagc
     2281 tggcgcttaa ggttccaacg acaaaattta aggcatagag tcctaatcca gaaatgataa
     2341 aggttgtcca taaccaagct aaatcaatct tgtctcggtc agattctcga gttgattctt
     2401 tgaggaggct aggaactgta ccgataatag ctcccgcaaa gctccataat acaaagacct
     2461 gataattttc aagcaggtat tcaatcgggt aggaaaataa gccgattccc agaagcatac
     2521 cgatggcaac tggaataaag tacaaaacat tttctttaag gtctttaaag ggatgggcca
     2581 gaaagccaat cattcgttca tagattccta agattgctgc tagaacccct ccggaaattc
     2641 ctggtaggat aaatccaaga gcaattacaa tccctttaat aacgcgtgct aaccatgaga
     2701 gcatatattt cctccaacta tttctatttc aaaaaatcct tactatattg tatcacaatc
     2761 agacacaaaa agaaaaagca aatgataaac aaatgctttt aaggttttaa aaaaagtttt
     2821 cgaaaggttt cttctttatt ttttaaggga gagataacgt tgatatctaa atcgtggtca
     2881 aagccggcaa tttttccttt agatgtgtat tggtgaatat cataatctaa atcagtttta
     2941 ggactgctct ccaaaaatcc tgagtctgag ccgtaggacg gaatccaaac agaggtaaac
     3001 ttgcctgtat caatactgtg ttcttccatg aagtagacac caacgtagat gccgatgttt
     3061 ttagcaccta gtgatgctag ttttgctcga aagttttcga caccttcgtt catattagac
     3121 atggttttgt cttccacgtc aagccaatag taactagggc tgtaaggaga ggcagcattg
     3181 tagaaaactt cggcagcctt ttccatttct tgggcacttt ttccagctac ataagcgtag
     3241 acagcaactg ggacattccg cttttgaagt tcagtgatat gactcttata ggccttgtct
     3301 attccattga taaatgaagc atcattttct tttgtcgttt gagcaccact gtgaacacga
     3361 acaatagcac ctgaaatatt ttgtgagagg gcatcgtagt tgatttcctc aggacgctgc
     3421 cagccagaga ggtcaataat cggtttgtct aagtgtttca aagcctgtgc ttcaatctgt
     3481 gctatattgg attttgtttt aaacgattgg ctgtcattaa gtgggcgatt gatgattaaa
     3541 atgaacatca taatccccaa aaaactaaat aaaataagtg gatgaatttg ttttctcata
     3601 tcttataatt ctaccctaaa aatcaaaaaa aatcaaaaaa atgggttaa
//
LOCUS       DAVKQL010000099         3548 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVKQL010000099 DAVKQL010000000
VERSION     DAVKQL010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.099"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            <1..170
                     /locus_tag="VW561_002025"
     CDS             <1..170
                     /locus_tag="VW561_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9944226.1"
                     /translation="TVSFAISSKGEKMKVENISYRVDHRILFDNISFDTSSSGVTLIT
                     GKNGTGKSTLL"
     gene            complement(536..1846)
                     /locus_tag="VW561_002026"
     CDS             complement(536..1846)
                     /locus_tag="VW561_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEU9944227.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            2083..2955
                     /locus_tag="VW561_002027"
     CDS             2083..2955
                     /locus_tag="VW561_002027"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEU9944228.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            3086..3548
                     /locus_tag="VW561_002028"
                     /pseudo
     CDS             3086..3548
                     /locus_tag="VW561_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1121 a          604 c          779 g         1044 t
ORIGIN      
        1 gaactgtttc ttttgctatt agttcaaaag gagaaaaaat gaaagtagaa aatatttcgt
       61 atagggtgga tcatcgtata ttgtttgata atatttcttt tgatacttcg agttcaggcg
      121 tgacattaat tactggtaaa aatggtacag gaaagtcaac tttactatag tagattgaaa
      181 ctagaatagt acacatctac ttctaaaata ttgttagaaa tcgatttgac tatcctgatc
      241 tatttgtcct gttcttattt catttcacta tatctcaaat tgagtatgac gaagtgcgct
      301 cccatgtcct gggaacgcac tttcttcata tttttcatat tcttgaatcc atcgataaag
      361 actattggga tgaattttta aagttgaact aatcattttt acaggatgag atttacagca
      421 gagagtttga aggctttatc aaaggttttt cttggcataa tgacttttcc tcgtttccac
      481 ttaattttgt gtctacttta ttataccaag tccaccctta agttagataa taaatctaac
      541 ttaaggaagc tagaaggatg agaatccagg tggtcaagag tcccaaactt aagctgatgg
      601 ggacacccag aataatttgc tttttgaagg caaggccacg ttcctctata ttgggaagtg
      661 agagttgaat gagagaacca gctgatgaaa agggtgagat attagtagat agagcgccaa
      721 taacggtggc tgttgtgagt aagtgaatat caatctgagg attttgagca ctgatgatag
      781 caatgatggg aaagagggct ggagctacaa cggatagggt ggaactaaag agtgacatca
      841 ctccggctat cacacaaaag aacagaggta accagaaatg aggaatggtt gttgtcatga
      901 ggtgccctat cagtgtgact aaacctgact tgaccgctag agacattagt aagctcatgc
      961 cgcagagcat gataattgta gcccagggaa ccttagctaa aatggcttct tgcttcccta
     1021 atttgagcct taaggcgagg cagaccatga gtattgagac aaagccaata tcaaatgttt
     1081 tttgataagt agctatccag gcgatgtttg ggaaaatgag atgcaacaag ggaaaaagcc
     1141 aaaccaaaac catgctgctg atcatgagca aggtggtttg tctttgaacc ttgctgagga
     1201 gtggtggttg gtcaatagtc aaggatgagt ttgttcttcc cttactatag tgactgtaac
     1261 aggataataa aagcaagacg atgagtgggt agataatgct gacgataaag atatgattgc
     1321 caagtgaaaa agcttgctct tcccatccca tttgcttaaa caggccttga aagacaatgc
     1381 ctgagctact ggttatcaaa ttagcccctc ctgaagctcc ccaattgacg gcttgagctc
     1441 caatcaaagg gtgtttgtcc gctttttgac agagggtaat cgctagagga cagcaaacgg
     1501 ccatagtagt gaaaaatcca gcacctaaag cagacaaaag ggttgccatc aggtataaaa
     1561 tcatgtagag ggcgttaggg tgggtgcgtg tgcggtagag aatgtgttga gccaaaacat
     1621 caagagtacc gttagttgtt gcaacgttat aaaagagaga gacgctaaaa atggtaaaaa
     1681 agagtgaggt tggccaaaaa tgaagaagtt ctttggggct taatcccatg agagtggttg
     1741 cgatgaggta agaaaaagca atagccagca ggccaatatt gattttggtg cggtaaccaa
     1801 ttccaatggc tagagcaatg gcgctaatca ttattaaatg aatcattgaa ttgtcctttc
     1861 agttagaata tggaaatagg ttggcaattt tggcgctcaa aagaccacct gtttcctatt
     1921 ggtacttgac tattatacac aaaaaaaata caattgtctt gacaattgta ttgacacttg
     1981 ttagaattaa aaatgaggta ttcaggcttg tgatttccta tggaagttaa tagtaattgc
     2041 ctctagtgct tacaagtgat attaaaaata gaggacttag tgatgtcaat catttcaact
     2101 gatttaaccc cttttcaaat agatgataca ttgaaagcag ccttgcgaga agatgttcat
     2161 tccgaagatt acagtaccaa tgccattttt gatcatcatg gccaagccaa ggtgtcgctt
     2221 tttgccaagg aagctggtgt tttagcggga ctaaccgttt ttcaaagggt ttttacccta
     2281 tttgatgccg aggtgacctt ccagaatcct catcaattta aggatgggga tcgtttgact
     2341 agtggcgatt tggttttaga aatcatagga tcggtgagaa gtctcttaac atgtgaacgc
     2401 gttgccttga attttttaca acatttatca gggattgctt cgatgacagc tgcttatgta
     2461 gaagccttag gcgatgattg cattaaggta tttgatactc gaaaaactac tcctaattta
     2521 cgtctttttg agaaatatgc cgtgagagtt ggcggtggct ataatcatcg ctttaattta
     2581 tcagatgcta tcatgctaaa agacaatcac attgcggcag taggtagtgt tcagaaggca
     2641 attgctcaag cgcgtgccta tgcccctttt gtgaaaatgg tcgaggtgga agtggaaagc
     2701 cttgctgctg ccgaagaagc tgcggcggcg ggtgctgata ttatcatgtt ggataatatg
     2761 tcattggaac agattgaaca ggccattacc ctaattgcag gacgttctcg gattgaatgt
     2821 tctggaaata ttgatatgac cactattagc cgttttcgtg gtttagcgat tgattacgtc
     2881 tccagtggta gtttaaccca tagtgctaag agtcttgatt tttccatgaa gggtttaacc
     2941 taccttgatg tctaagttgt aaaataaact aactttttaa aggatgtctt tcctctagaa
     3001 cgagttttat gtcagatagt ttaaacgcct cttcaaatat agtaaaatga accaaaaata
     3061 gtacacaatg aggtataatc ttcttatggc atattcaata gattttcgta aaaaagttct
     3121 ctcttattgt gagcgaacag gtagtataac agaagcatca cacgttttcc aaatctcacg
     3181 taataccatt tatggctggt taaagctaaa agagaaaaca ggagagctaa accaccaagt
     3241 aaaaggaaca aaaccaagaa aagttgatag agatagactt aaaaactatc ttactgacaa
     3301 tccagatgct tatttgactg aaatagcttc tgaatttggc tgtcatccaa ctaccatcca
     3361 ctatgcgctc aaagctatgg gctacactcg aaaaaaaaga accacaccta ctatgaacaa
     3421 gacccagaaa aagtagcctt atttcttaag aattttaata gtttaaagca cctagcacct
     3481 gtttagattg atgaaacagg attcgatact tatttttatc gagaatatgg tcgctcatta
     3541 aaaggtca
//
LOCUS       DAVKQL010000100         3475 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVKQL010000100 DAVKQL010000000
VERSION     DAVKQL010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.100"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(183..794)
                     /gene="rpsD"
                     /locus_tag="VW561_002029"
     CDS             complement(183..794)
                     /gene="rpsD"
                     /locus_tag="VW561_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU9944229.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(989..2041)
                     /locus_tag="VW561_002030"
     CDS             complement(989..2041)
                     /locus_tag="VW561_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU9944230.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVTKLEKGQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2038..2736)
                     /locus_tag="VW561_002031"
     CDS             complement(2038..2736)
                     /locus_tag="VW561_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9944231.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(2903..>3475)
                     /locus_tag="VW561_002032"
     CDS             complement(2903..>3475)
                     /locus_tag="VW561_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat-containing
                     protein"
                     /protein_id="HEU9944232.1"
                     /translation="NGDAKNPALSPLGENVKTKGQYFYQVALDGNVAGKEKQALIDQF
                     RANGTQTYSATVNVYGNKDGKPDLDNIVATKKVTIKINVKETSDTANGSLSPSNSGSG
                     VTPMNHNHATGTTDSMPADTMTSSTNTMAGENMAASANKMSDTMMSEDKAMLPNTGEA
                     QTSMASIGFLGLALAGLLGGLGLKNKKEEN"
BASE COUNT          955 a          779 c          671 g         1070 t
ORIGIN      
        1 aggattatac cacattgtgt actatttttg gttcatttta ctatatcact ttggactata
       61 tcggtctagt tatgtctaaa cgattatagc tggatactaa gagaaatcaa aaaagcacta
      121 aaggagaacg tcccaaagtg cttaatatca aggcctcaaa gcctgtctta ttcaataaaa
      181 tattacaaca tcttgttgta gaattcaacg acaagtgctt cgttgatttc tgggttgatt
      241 tcgtcgcgtt ctggcaagcg agtcaatgaa ccttccaatt tttcagcgtc gaatgatacg
      301 aatgctggac gtccaagagt agcttctact gcttcaagga ttgctggaac tttcaatgat
      361 ttttcacgaa ctgagatcac ttgacctgga gttacgcggt atgatgggat atcaacgcgt
      421 ttcccgtcaa caaggatgtg accgtggttt acgaattgac gagcttgacg acgagtagtc
      481 gcgagaccaa gacggtaaac aacattatcc aaacgacgtt ccaaaagaag cataaagttg
      541 aaacctagga ttccaccttt gatttttgta gcttgtacga acaagttacg gaattgtttt
      601 tcacctacac cgtaagtgaa acgaagtttt tgtttttcag ccaattgcaa accatattct
      661 gacaatttag aacggttgtt tggtccgtgt tgtcctggta cgtagttacg acgtgccaat
      721 tctttacctg tacctgtaag tgaaaggcca aggcgacgag cttgtttcca agatggtcct
      781 gtataacgtg acatatgaat gtcctcctga tataaataat atttcggtgg aaatagtcac
      841 ttagaaagcc ctgattcgtg cagatgccct tcgcctaaac agccaaggtt acttgtcata
      901 agacacctgt tgacgagctt catgctttcc tgctgatatt tcacacaaaa tccattttac
      961 catgaatagc tggatttgta aaggggtttt aggctttatt ttcactacca gagagattga
     1021 gaacgagggt aaaggtactt cctagaccgt actggctgct gactgtgatt tccccaccca
     1081 attgatgggc caattcacgc gcaatcgcaa gtcctaatcc atgaccacct gtcttcatgt
     1141 tacgcgaagt ttcgacacga taaaggcgtt tgaaaatatt ttccaaatcc tctggggcaa
     1201 tgccctgccc ttcatcggtc acactgattg aaagctggcc cttctccagc ttagtcacca
     1261 cttccagctt ggttcctgga gcagaatatt taaaagcgtt atcgaccaga ttcaccaaga
     1321 tacgagaaag cttagcataa tctccctcaa tccgggcaga ctctgggatt acttgcaagt
     1381 ggacatctct tctctcctgc tcaatcaaaa actgaaattc actcatgcac tcaattaaga
     1441 gcttgtccag aaaaatactg tctttactgg tagtttccac ctgatttcta gctgtattta
     1501 gggtcaaaaa attcaactcc tcaaccagtt tattgagcct ctccgtctgg cgtccaatgg
     1561 ttgctagata atgagcttgc tccgactcct tgataatccc atccaaaatc ccttctaccg
     1621 tcgcttggat cgaagtgata ggagtcttaa tatcatgcga caactgggca atcatcaagc
     1681 ccttttctcg ttcgctttct tccaaggaat caaagcttac ctgcaaatca tgggacatct
     1741 cattaaaagt ttgccctaat tgctgaaatt ctacaggacc ttgaacctcc aaatttgaag
     1801 gaaaatcctt ggccgctacc cgcttggcat gctccttgag tttgcccaac gacgtaaaga
     1861 ctggcaatag gagaaagaga ctaatcccag caccgacaag gctggcaacg atggtcatcc
     1921 caagcaaaaa gtaaatctcg cctttcgcaa tcagcatttt ttgaacagcc caaaaaacaa
     1981 ccaaaatggt taagagggtt gaaataatat atccaaccaa aatataactt tttagtttca
     2041 tgtttgtcct ctcggtttct ctatcttata tcccaacccc caaactgtct taatagtggg
     2101 cgtttggtca ctactatatt ttgccagctc ctgtcgaaga gcatggatat gcacattcaa
     2161 ggtattggtg tcatccacgt agtcttcttt ccagatcttt tcatagaggt ctgtcttgga
     2221 gaaaactcgc tctggattgc tagctaaaat ccacagcaat tcaaatgatt taacagttaa
     2281 atccagcatt tcttctccta tttgaacttc atgactacta tgattcattt ttagattgcc
     2341 aagggaaatc agctctgttt cgcccccacg atgaaggcgg cgcaaaatat tgtggacacg
     2401 caaaaccagc tcacgtgggc taaaaggctt agcaataaaa tcatctgccc ccaagctcag
     2461 gccgtaaatc ttgtcctgtt cactggtctt agcagtaata aataggaaag gctgctctgg
     2521 tgataagtat tgaacctcac tgattaaatc ataaccatcc atccgaggca tcatgacatc
     2581 tgtgataatc aaatcaatcg gttttttctt gaacagctct agcgcttcca gtccatcatg
     2641 ggctaccaag acctgataac ctgcctgaat taagtatctc tgatgaatat ctgtgatttc
     2701 tacctcgtcg tcaacgagta aaattgtctt tcccatctgt ctctcctttg ataaaaacag
     2761 tgctatactg ctttaagtat aacactattt ttattgtttt acgatgattt taatctgcta
     2821 ttgaaagaaa gtcctgattg ctgattgagt tgttaaacta atcttagtca gcccactatc
     2881 catcatttat ttccttagct gattagtttt cttctttttt gtttttcaaa cctagaccac
     2941 cgagtaaacc tgcaagcgca agtccaagga aaccaatact tgccattgat gtttgagcct
     3001 caccagtatt tggtagcata gctttatcct ctgacatcat cgtatcagac atcttgttag
     3061 cagaagcagc catattttca cctgccatcg tgttggtaga acttgtcatg gtgtcagcag
     3121 gcatgctatc tgtagtacct gtagcatgat tgtgattcat cggagtcacg ccagaaccag
     3181 agttagaagg tgataatgaa ccatttgctg tgtctgatgt ttctttaaca tttatcttaa
     3241 tagtgacttt tttagttgct acgatgttgt ccaagtctgg tttaccgtct ttgttaccat
     3301 agacattgac tgtagcactg taagtttgag taccatttgc tcggaactgg tcaatgagcg
     3361 cttgtttttc tttgccagct acatttccgt ccaaggctac ttgatagaag tattgacctt
     3421 tggtcttcac gttttcacct agtggagata gggctgggtt tttagcgtca ccgtt
//
LOCUS       DAVKQL010000101         3451 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVKQL010000101 DAVKQL010000000
VERSION     DAVKQL010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.101"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(37..471)
                     /locus_tag="VW561_002033"
     CDS             complement(37..471)
                     /locus_tag="VW561_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU9944233.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAEDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNER"
     gene            complement(485..1945)
                     /locus_tag="VW561_002034"
     CDS             complement(485..1945)
                     /locus_tag="VW561_002034"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU9944234.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            complement(2069..3418)
                     /locus_tag="VW561_002035"
     CDS             complement(2069..3418)
                     /locus_tag="VW561_002035"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEU9944235.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWETFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
BASE COUNT          912 a          724 c          672 g         1143 t
ORIGIN      
        1 cttatagtaa aaaaagccag aattatactg actcttctat cgctcattaa acttagaagc
       61 acgttcttct ccccaccaat aagggattag ttctgcgact ttaactgttt ttcttatatt
      121 atagtccatc atgaattctg catctttatt ttcagcatta agctctaaaa ggaattctct
      181 acaagcaccg caaggcatgg ctgaacttcc accataaggt ggtttgtctc gaaaggctaa
      241 tactttctta accttagttt gtcctgaaaa ttggtacata ttgaagaggg ccgcccgttc
      301 tgcgcagaga tggaaaacac cacaggttcc ctccatacag aatcctgtaa atatttgtcc
      361 atcttctgct tctactgcag ctacaacatg attggcataa acaaagtctg atacttcatg
      421 tggattgtat agtttctgtg cttcttcgta catcttttcc cagatgtcca ttattgtatc
      481 ctctttattt agagatttct tttagcatgt tttcgatatg ctgaattgat ttttcacgtc
      541 caagcaagaa aattgtatct ggtaattctg gcccatgcat ttcgcctgaa actgcgatac
      601 gaataggcat gaaaagattt ttccctttaa tacctgtttc tttttgaact gctttaattt
      661 gtgggaagat attttctgtc acaaatttat catctgtcat cgcttcaagt tttgctttga
      721 atgcttcaag aactgttgga actgtttcac ccgtcatgac ttcgcgctct gcttctgtca
      781 attctgggaa atctgagaag aaaagatctg tcaatgggat aatctcatct actgatttca
      841 tttgtggttt atagagctca actaattttt cagccttgtc agtcaaacgg cctgcttcct
      901 ctaagaatgg ttttgccatt tcaaagatgg tttcaaggtc tgcattcttg atataatcat
      961 tgctcatcca gtctagtttt ttctgatcaa aggctgctgg tgacttgctg aggcggtttt
     1021 catcaaaaag tttaatgagt tcttcacgag agaaaatctc atcttcacca cctgggttcc
     1081 aaccaagaag agcaataaag ttaaagactg cttctggaag gtaacctttc tttcggtaat
     1141 cttcgataaa ttgaagtgta ttagtatcac gtttagataa cttcttacca gtctcagagt
     1201 tgataatcaa ggtcatgtga ccgaactctg gagcttccca accaagagct tcatagacca
     1261 taagctgttt tggtgtatta gcaatatggt catctccacg gataacatga gagatttgca
     1321 tatcgtggtc atcgataaca acggcaaagt tgtaagttgg gtaaccgtct ttcttttgga
     1381 taacccagtc accaccgata ttgccacctt caaattcgat atcgcctttg accatatcat
     1441 gccacttgta gatacctgac tcattgacag ccaaacgaac agttgggatg atccctgctg
     1501 cttcacgttc tgcgatgtaa gctgcttttt cttcttcact cataccaagg tattcattga
     1561 tgtagcgtgg tgtttcgcca gctacttctt ggcgttcgcg ttcagctgcc aactcttctt
     1621 ctgtaacgta agatttatag gcttttcctt cagctaatag ttggtcaatg tatttttgat
     1681 acaagtctaa acgctcagac tggcgataat tctcatgtga ttctggactt tcatcccaat
     1741 ccatgcctaa ccagcgaagg ttttcaagtt gtgaacgttc accatcctcg acatggcgtt
     1801 tacggtcagt atcttcgata cggatgagaa atgttccacc atgatggcgc gcatacaagt
     1861 aattaaacaa tgctgtacga gcatttccga tgtgtagtag tcctgttgga cttggtgcgt
     1921 aacgtacgcg gatatctttt gacatagttt ctcctattat ctaggcatct gcctttttgc
     1981 tttctatatt atatcacaag tcttgactga gtccaatttc tatggataaa gagagtaaaa
     2041 agagtgggta aaccactctt ttcttctatt atagacgtgc gttaagttct ttgctcaatt
     2101 cttcaaatcc tggttttcca agaagggcaa acatgttacg tttataagct tcaacacctg
     2161 gttggtcaaa tgggttgata gcattcaagt aacctgaaag ggcaattgcc aattcgaaga
     2221 agtagatagt gtaaccaaga gtgaaagcgt cttgctctgg aagagtcaca tacatgtttg
     2281 gtacatcacc atctgtgtgg gcaagaagaa caccgtcagt tgcttttttg tttacaaagt
     2341 caacgtcttt tccttgaagg taaccaagtc cgtcaaggtc ttcttccaaa gtaggaataa
     2401 gcacgttttt acgaggtttg tcaacacgga caactgtttc aaacatgata cgagttcctt
     2461 cttggataaa ttgaccaagt gagtgcaagt cagttgagaa gttggctgaa gttgggtaga
     2521 tacctttttg gtcttttcct tctgattcac cagccaattg tttccaccat tctgagaagt
     2581 attgaagtga tggctcatag tttaccaaga tctcagttgc atagccttta cgataaagga
     2641 tgttacgaac agctgcgtat tggtaagctt cgttttcaga gattttgtct gaagtgtagt
     2701 ctttgcgagc tgcattcgca ccttccataa gagctttgat gtcagctcct gatgctgcga
     2761 ttggaagcaa accaacggct gtcaatactg agaagcgtcc accgatatca tctggaacaa
     2821 caaatgtttc ccaaccgtta gcgtctgctt caaccttaac agcacccttt tggcggtcag
     2881 ttgttgcata gatacgtttg ttagcttctt cttgaccgta tttcttaacc aagagttctt
     2941 taaagacacg gaaagcaatc gctggttcag ttgttgtacc tgatttagaa attacgttta
     3001 ctgagaagtc tttgtctgca acatactcta ccaagtcagc aaggtaagta gatgagattg
     3061 agtttcctgc gtaaaggatt tgtggagctt tgcgttcttc ttttgtttgc aagttagcaa
     3121 aatggtgatt caagaagtcg attgctgctt ttgcaccaag gtaagatcca ccaataccga
     3181 tcacaaccaa aacatcgctg tctgacttga tttgctcagc agctttcaag atgcggtcaa
     3241 attcttcgcg gtcgtatttt tcaggaaggt ccaaccatcc caagaagtcg ctaccagcac
     3301 cagttccttt acggatcaat tcatctgctg ctgttacttg tgattgcatg tattccactt
     3361 catgtggtgc aacaaatttg tctaaaactt ttgaataatc aaatttaata tgtgacatgt
     3421 tcttcctcca atatttcttc ttttctatca t
//
LOCUS       DAVKQL010000102         3192 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVKQL010000102 DAVKQL010000000
VERSION     DAVKQL010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.102"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            147..1658
                     /locus_tag="VW561_002036"
     CDS             147..1658
                     /locus_tag="VW561_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEU9944236.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            1680..3011
                     /locus_tag="VW561_002037"
     CDS             1680..3011
                     /locus_tag="VW561_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU9944237.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNDLKEAGYDYQSTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVKLLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            3106..>3192
                     /locus_tag="VW561_002038"
     CDS             3106..>3192
                     /locus_tag="VW561_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9944238.1"
                     /translation="MKLSYEDKVQIYELRKQGQSFKQLSKRFG"
BASE COUNT          887 a          663 c          683 g          959 t
ORIGIN      
        1 ttgaaaacat gtacaatatt tcaaaagata ctagttttag actttaatat ggtaaaacaa
       61 atataaatag aaagcgtttt cttgaatgtt tatttaagaa agtagttggt tttttacact
      121 ttgttagaca tcaggaggaa aaacaaatga gtgaaaaagc taaaaaaggg tttaagatgc
      181 cttcatctta caccgtatta ttgataatca ttgctattat ggcagtgcta acttggttta
      241 tccctgcggg ggcctttata gaaggtattt acgagactca gcctcaaaat ccacaaggga
      301 tttgggatgt cctcatggca ccgattcggg ctatgctagg tactcatcca gaggaaggtt
      361 cgctcattaa agaaacgagc gcagcgattg atgtagcctt cttcatcctt atggttgggg
      421 gtttccttgg cattgtcaac aaaactggtg ctcttgacgt agggattgcc tctatcgtga
      481 agaagtataa gggccgcgaa aaaatgttaa ttttggtact gatgcctttg tttgccctcg
      541 gtggtacaac ttatggtatg ggggaagaaa caatggcctt ctatccactc cttgtgccag
      601 ttatgatggc cgttggtttt gatagcctga ctggtgttgc aattattttg ctcggttctc
      661 aaatcggctg tttggcatct actctgaatc catttgcgac aggtattgct tcagcgactg
      721 cgggagttgg tacaggggac ggtatcgtac ttcgtctgat cttctgggtt accttgactg
      781 ctcttagtac ttggtttgtt taccgttatg cggataagat tcaaaaagat ccgactaagt
      841 cactggttta tagtactcgc aaagaagatt tgaaacactt taacgtagaa gaatcttcat
      901 ctgtagaatc tacacttagc agcaaacaaa aatcagttct cttcttattt gtgttgacat
      961 tcatcttgat ggtattgagc ttcattccat ggacagacct tggcgttacc atttttgatg
     1021 actttaatgc ttggttgact ggtcttccag ttattggtaa tattgtcggt tcatctactt
     1081 ctgcactagg tacttggtac ttcccagaag gcgcaatgct ctttgccttt atgggtatcc
     1141 tgattggtgt tatttatggt cttaaagaag ataagattat ctcttccttc atgaatggtg
     1201 ctgctgactt gctcagtgtt gccttgatcg tagcgattgc tcgtggtatt caagttatca
     1261 tgaacgacgg tatgattacc gatacaatcc tcaactgggg taaagaaggc ttgagcggtc
     1321 tatcttcaca agtctttatc gttgtaactt atatcttcta tctacctatg tcattcttga
     1381 tcccatcttc atctggtctt gccagcgcaa ctatgggtat catggctcca cttggagaat
     1441 ttgtaaatgt ccgtcctagc ttgattatca ctgcttacca atctgcttca ggtgtcttga
     1501 acttgattgc accaacatct ggtattgtga tgggagctct tgcacttgga cgtatcaaca
     1561 ttggtacttg gtggaaattc atgggcaaac tcgtagtcgc tattattgta gtgaccatcg
     1621 cccttcttct ccttggaacc ttccttccat tcctataaaa tagtgagtga ggtgattcca
     1681 tgaaaataga tataacaaat caagttaaag atgaatttct tatatcatta aaaaccttga
     1741 tttcctatcc ttcagtactc aatgaaggag aaaatggaac accttttgga caagcaatcc
     1801 aagatgtcct agaaaaaact ttagagattt gtcgagacat aggtttcact acctatcttg
     1861 accctaaagg ttattacgga tatgcagaaa tcggtcaggg agcagagctt ctggccattc
     1921 tctgtcattt ggatgttgtt ccatcaggtg atgaagcaga ttggcagaca ccgccatttg
     1981 aagcaactat caaagacggc tgggtattcg gacgtggtgt ccaagatgat aaaggtcctt
     2041 cgcttgcagc tctctatgca gtaaaaagct tgctggacca aggtattcag ttcaaaaagc
     2101 gcgtacgctt tatctttggt accgatgagg aaaccctctg gcgctgcatg gcacgctaca
     2161 ataccatcga agaacaggcc agcatgggct ttgcacctga ctcatctttt cctctgacct
     2221 atgctgaaaa agggcttcta caggtcaaac ttcatggccc tggatcggat caactagagc
     2281 ttgaagtagg aggcgccttt aacgttgtac cagacaaggc caactaccaa ggtcccctct
     2341 atgaacaggt ttgtaacgat ctcaaagaag ctggttatga ttaccaatcc actgaacaaa
     2401 ccgtaacggt tctcggagtg ccaaagcatg ctaaggatgc tagtcaaggt atcaatgctg
     2461 tcatccgact agctaccatt cttgctcctc tccaagaaca ccctgctctc agctttcttg
     2521 caacacaagc aggtcaagac ggcacaggaa gacaaatctt tggtgatata gcagatgaac
     2581 cttctggtca cctatccttt aatgtcgcag gtctcatgat caatcatgaa cgttctgaaa
     2641 tccgtattga cattcggact cctgtcttag ctgacaagga agaactagta aagttgctta
     2701 caagatgtgc acaaaactac caactccgct acgaagagtt tgactatcta gcgcctctat
     2761 acgtcgcaaa agacagtaaa ctcgttagca cactgatgca aatctaccaa gaaaagactg
     2821 gcgataacag tcctgctatt tcatccggtg gtgccacttt tgctcgcacc atgccaaatt
     2881 gtgtagcctt cggcgcctta ttcccaggag cgaagcagac agaacatcag gcaaatgaat
     2941 gtgccgttct agaagatttg taccgtgcta tggatattta tgccgaagcc gtctatcgac
     3001 ttgcaactta atcaggcaac tgtttctaca aaaaaaaatc gaccgattaa tgaactgcac
     3061 cccaaaagtt agacagaata aatctaactt ttggggtgtt ttattatgaa attgagttat
     3121 gaagataaag ttcagatcta tgaactaaga aagcaaggac aaagcttcaa acagctttca
     3181 aaaagatttg gt
//
LOCUS       DAVKQL010000103         3115 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVKQL010000103 DAVKQL010000000
VERSION     DAVKQL010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.103"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(21..1376)
                     /gene="rlmD"
                     /locus_tag="VW561_002039"
     CDS             complement(21..1376)
                     /gene="rlmD"
                     /locus_tag="VW561_002039"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU9944239.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIAKRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
     gene            1414..2190
                     /gene="recX"
                     /locus_tag="VW561_002040"
     CDS             1414..2190
                     /gene="recX"
                     /locus_tag="VW561_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEU9944240.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINTNQLSGDKGPYVLTQKLAQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPSRALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLSQVLARKGYDFSDIASALREYL"
     gene            2279..2812
                     /locus_tag="VW561_002041"
     CDS             2279..2812
                     /locus_tag="VW561_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEU9944241.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
BASE COUNT         1015 a          558 c          545 g          997 t
ORIGIN      
        1 atgttttgtc aacacctttt ttaaactttt tttattaatt ttacaacagc ttcagttcga
       61 gctgtatgtg ggaacatatc gaccgactgg atataatgaa gatcatagac ttctactaag
      121 cgtaccaaat cacgagccaa ggtcgaaaca ttacaagaaa tataaaccat tttttctggt
      181 acataagtaa gaatagtatc taataactta tcatccagac ctgtacgtgg tgggtcaaca
      241 atcaaagcat ctgctcggta gccttccttg taccaacgag gaataatctc ttctgccgtt
      301 ccagcttcat aatgagtatt gtcaaatccc attcttttag catttcgctt ggcatcttca
      361 atagcttctg gaataatatc catacctctg agtgttttta ctttctttgc aaaggcaaat
      421 ccaatcgttc caactccaca ataagcgtca atcaaatggt cttctttatc aacatccagc
      481 gcttttactg cttcgctata gaggacttct gtttgctcag gatttagttg ataaaaagct
      541 ctaggggata gtgaaaattc ataattgagt acaccttctt gaatactctc ttgcccccag
      601 ataatctctg tcttttcacc atatatctca ctggttttag ctgtatttgt attaacagct
      661 actgtcacaa cttctgggaa atctttaacc aactctttta ccaattgagt taaattaagc
      721 tggcggtttg taacaataat aatctgaacc tgtccggtct ttctcgcgcg tcggaccata
      781 atagtacgga cacctagaac ttttctctca tccgtgattg gaatctggtg ataagtaagt
      841 aattctgcta agcgcttagc aatcacttgg gtttccttat cttgtaccag gcagtctttc
      901 aactctacta aatagtgaga gttttgtgca tataagcccg ccttgacctg atttttaaat
      961 tttcgagtct gaaattgtaa cttagctctg taatattttg gttcctgcat tccaatagtt
     1021 ggacgaattt cataattttc atatcctgca ggagcaaatt ttttcagcgc ttgatgaagt
     1081 aagtccgtct tgaactccag ctgcttatca taatgcaggt gcatgatttg gcagcctccg
     1141 cattcattat aaatagtaca agatggcaca attcgaaatt tagacttctt gttgaccttc
     1201 agtaattttg cttcaacaaa gttgcgtcta atagaagtaa tctgacaata gatatcttcg
     1261 cctttgagag ctcctggtac aaagactaat gttttttggt aaaagccgat tccctcaccg
     1321 ttaattccca tgcgcttgat ttttaatggt attttttgtt tcactttcag attcataccc
     1381 ctatcttatc acattttgag ttataataga gctatgaaaa tcacaaaact tgaaaagaaa
     1441 aaaagactct atctgatgga gcttgataat ggcgacaaat gctatatcac cgaagataca
     1501 attgttcgtt ttatgttatc gagagataag gtgataagcg aagaggaatt gaaagagatt
     1561 caagactttg ctcaattttc ttatggtaag aatctggccc tctaccactt atcctttaaa
     1621 gcacgcactg aaaaagaagt cagagaatat ctgaaaaaat acgatattga taaaaacatt
     1681 gtttctcaag ttattgctaa tcttaaagaa gataagtgga ttaatgatgg tcagtacgct
     1741 tatgctatca tcaataccaa tcaactttca ggagacaagg gtccttatgt gctgactcaa
     1801 aaactagctc aaaaagggat ttcaaaatct actatagaag agaacttgaa agaatttgat
     1861 ttttctgaag ttgctcaacg tgtagctaat aaactattga aaaaatatga gggaaaactt
     1921 ccatctcgtg ccctgcaaga taagattatc cagaacttga ctaacaaggg cttctcttat
     1981 tctgatgcta aaattgcctt tgacgactta gatagccaag ttgaccaaga aacgactcaa
     2041 gaactcatct tcaaggaact tgataagcaa tatactaagt atgctcgaaa gtatgaagga
     2101 tacgaactga agcagcgttt aagtcaagtt ttagcacgaa agggctacga tttttcggat
     2161 atagcaagcg ctcttagaga atatctttaa tatttccaag tgaaattcac agattttagg
     2221 taattttatg gtacaatagt aaatgataaa cttataaatt gtagaaagtt ggttagttat
     2281 gaagcttcca aaagaaggcg actttattac aattcaaagt tataagcatg atgggagtct
     2341 tcaccgaact tggcgggaca ccatggtact aaaaacaaca gaaaacgcca ttattggtgt
     2401 taacgatcat acacttgtta ccgaaagtga tggtcgtcgt tgggtcactc gagaaccggc
     2461 tattgtttac tttcacaaga aatattggtt taatatcatt gccatgattc gtgataatgg
     2521 aacttcttac tattgcaata tggctagccc ctactatctg gatgaagaag cactgaagta
     2581 tattgattac gatttggatg ttaaaatttt tacagatggt gaaaaacgtc tcttggacgt
     2641 tgaagagtac gagcgtcaca aacgcaaaat gaattattct gatgacttgg actatatttt
     2701 aaaagaacat gtcaaaattc ttgttgattg gattaacaat ggacgtggtc ctttctcaga
     2761 agcctatgta aacatttggt acaagcgcta tgtagaacta aagaatcggt aaagttgtca
     2821 aaggtcagga acaaaatcct gacctttttt tggtagtata aaataaattg tgtaaacaca
     2881 aaaaggaata aatccattat agcagagtta caacacatta ctagaaagag atttatgcag
     2941 aattcgtaaa atatataaaa caaatagata tactgttatt ttttaatgtc tttattcctt
     3001 gtaagtttac aacaaactta tatcgtttaa tacaaaaaat acgaaaatga ttagtataaa
     3061 aaatacaggt caacttcgta tcaaaaacat tttttgtatt tccacatagc atcaa
//
LOCUS       DAVKQL010000104         2927 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVKQL010000104 DAVKQL010000000
VERSION     DAVKQL010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.104"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(404..859)
                     /locus_tag="VW561_002042"
     CDS             complement(404..859)
                     /locus_tag="VW561_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944242.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSGNLQP
                     EKWNETAKQLYNIIMWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKRVNTTIKN
                     GSKGKTLTEMINGYRADNDIRDEICNFNILKNKIRDMKDQQGNTMESYF"
     gene            complement(1134..2543)
                     /locus_tag="VW561_002043"
                     /pseudo
     CDS             complement(1134..2543)
                     /locus_tag="VW561_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
BASE COUNT          920 a          575 c          422 g         1010 t
ORIGIN      
        1 aagcataacg caccaaaaat ggtgcgttat ttcttgtttt tctatcctaa actttacttc
       61 tataaaagtc acctaaagag ccataaatat ttattcttgt attaattttt tcgatgcgca
      121 aacgatatct acaaatacat ctataacgat tcacctttta ggatttttat tgtcaaaatt
      181 gcctttatct acaaagacaa aatgcttata aaatagcttg aaatcaagga ttatcacctt
      241 tttcgttgta gcaacactcc acatgtgatt attaggctat tagcaaagcg tatccaatac
      301 ctcaataaag tgttgttttt actgatattt taactatatt gtatagtcta ctatttaaat
      361 ctcaattagg atattttata gaatacccaa aattcaacaa taactaaaag taagattcca
      421 ttgtatttcc ttgttggtct ttcatatcac gaattttatt tttcagaata ttaaagttac
      481 aaatttcatc tctaatatcg ttatccgctc tgtagccatt aatcatctca gtcagagttt
      541 tccctttact accattcttt atagtagtat ttactctttt tatactgctt tcaaaaggct
      601 catatgtttt atacttaatt acttcgtctt taaaattaaa taaaggtgta tccggcttag
      661 cattccataa aataatcaac cacattataa tattataaag ctgctttgca gtttcattcc
      721 acttttcagg ttgtaaattt ccactccctg tattgtcata ttcttgtcta ttatttacat
      781 gagaatcacc tacaagcgga ttaaaaagag gtagcccttt gtgattaatt agttctaaaa
      841 aatcccttgc cgcacccatg caacactgaa taacttcctt cttgccatca attttactct
      901 cagcgataaa tacataatat tcatcttcaa tagttgctcc tgcatcacta tgtttttgtt
      961 gatttgctaa cagcttaata tgtgctttag gctaaacttc aaaatctcta acaattcttt
     1021 gtcttgtttc atgtcctcta caattcattt aagttctcct ttacaagttt agtaactagc
     1081 cttattatac tatataagat atgaaatgta ccataatctt catcaactag tttttagaaa
     1141 atcacaaatc catctggaga gatgacaagc tgattctcca attcttggca attgcttgct
     1201 agtgctactg cttctttctc taaaagataa tcttctgttg attggttgaa tattactttg
     1261 aggatccgtc cttcgtattt ccattccaga gctactagat cagatttgat ttcttgaagg
     1321 ctatacttgc catgcgaaat gattactgac gcgtatttcc gaattttaat cagcctcttc
     1381 ataaagttca gcatatcatt gtcacttgat acacgttccc aaggcataca acgacgacaa
     1441 tctggatctg gtcctccagt caaggctagc tcggttccgt aataaatgca cggtgttcct
     1501 ttttgtaaaa agagaaaggc taaggctgat ttaaccagtt gaacatcttc attggccgtc
     1561 cacaggattc gctctgtatc atgtgaatcc aagagattaa acatgacctc tggattggct
     1621 gtatttagct taggcatata gtcctcgaaa ccaaaaacat gatagggtaa gtctctctta
     1681 ttaactagct tttcagttgt cactgggaat tgttgaatat ggaaccaatc cttataagca
     1741 gactgttcac catttttgac gacatttttt cattgaagag attgcgaacc aatatgatta
     1801 aataccgcat ccagcatgac tttcatgcca cgatgatgcg cttgatccac cagttcccga
     1861 aaggtctcct tgtctccaaa atgacggtca atttcaaagt aatctgtcgt attgtacttg
     1921 tgattgcttg tagattcaaa gatgggacaa agatatagtc cagtaatacc caagtcttgc
     1981 aagtaatcca tatgatcaat aatcccctgt aaatcaccac caaagaaatc atcgctctta
     2041 ggtgtgacag atgaatccca gtctaaagtc ccttctgggt ttaatagagc attgccattg
     2101 gcaaatcttt caggaaatat ctgataccat accgtatttg aaacccagtc aggaaccttg
     2161 caggcatcaa tctcatgaag ataaggcaac ttaaatccat tcccaattgc atgaagattt
     2221 tctagagaat tttccacaca ccctttatcg ccatacaaaa tattttgacc ttctgtatct
     2281 ctgagctcaa agagatactg gatacgtgca aagtcaactg acacttcaac ctgccaatgg
     2341 tcaaataagg taccagaagt tatcttgacc atttcttttg tatcctgata aaactcctcc
     2401 ataaagataa aagggtcccc atagtgcaag ttgatgcttt caatgtcccc tttcttagtt
     2461 cgaattcgaa tatggagttt cttatcctta taaagatagg catactccga ctcaggccta
     2521 tggtaaatag cactcaattc catcaatttg tctcctaaag tattcattca aattttacgc
     2581 aaacgttttc aaactcaatg aatccagtat actatttttg taaactattt gcaaggaatg
     2641 tccgttgtta aaggaactat cttttaaagt atacaaaaag ccttaaaaaa ggctttgaat
     2701 tactactatt gattagaagg atataaagtt tacaagtatg gtacaatcta ttcaaattta
     2761 attagtaatt ttgaattcca atttcatata gactcaagat agtagtatat atttgaagat
     2821 agaaaagaag acatttaatt aatctttgtt atctcctaaa aaaatcatga ttgcttgtcc
     2881 atcaactcca gaattataca tagtccaata tatagatttt gtttcaa
//
LOCUS       DAVKQL010000105         2678 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVKQL010000105 DAVKQL010000000
VERSION     DAVKQL010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.105"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            516..1199
                     /locus_tag="VW561_002044"
     CDS             516..1199
                     /locus_tag="VW561_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9944243.1"
                     /translation="MKEKKVYQILAIDDDESILRLIKNSLERSEFEVTTRREVGNIDI
                     CSFIGFDLILLDIMMSIDGLEICKSIREQIDVPIIFVTAKQLDSDLASGVKAGADDYI
                     KKPFSITELVARVRMHIQREERNRKNNKEICTKNLIINVSNQEILIEEETISLTKREF
                     TILYTLASNPKRVYSVEELYERIYPSESDAQFRSIAEYIYQIRSKLKPYSINPIKTQW
                     GGGYSWNEN"
     gene            1184..2281
                     /locus_tag="VW561_002045"
     CDS             1184..2281
                     /locus_tag="VW561_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU9944244.1"
                     /translation="MERKLDFKTLVKKTKWTIVKQFCLNIIVAYIFPISTISIDIHSE
                     TQSIARDLLGVLFNVFSWVYICISLIIIVSINIKALLTRVKQEMEIVYHSSIWLTSVN
                     DSSNLTILEFIETNRHIESMQQRIKNMIQVEKDQKADILFKVSAMAHDLKTPLTVIKG
                     NSELLQLAPLSGIDVQCLKDIERASNQLNSYFNQLINYSKTFYSDQISFHQYRISNLS
                     KILEQECTYLIGDSIDYKYSTKIDRDCNIELDLDLIIRSVANIVNNAIAYADDAKKKI
                     RVTFELADSNITLSIWNNGSKFSDDVLENFGKLFYREDTSRNSQFEHFGIGLAFVKQV
                     MSIHSGDIQLQNKENGAMVKLIIPMKSSDYV"
BASE COUNT          947 a          334 c          463 g          934 t
ORIGIN      
        1 acggttcaac tttaagaagc agtttattag ctaatccaac acgttggaaa ttctttttta
       61 taaaattaat agtaatatta tcaatatcta tattagtttg gactctggtt gctttctgta
      121 ctatttgcgt agtgtatatt ttttatggtc tattaattcc cttacttatt tataccttta
      181 cattaagaat aatattggta tcaattggtt taatattaat atgttttagt ttagtgatta
      241 taacaaggag tatagttgtt ccaatgggga tgtctttgtc ttttttgttg gggttgggtc
      301 aaatgcttct gcaattcagc gatgcttttc tctacttccc aattatctcc actatgaatt
      361 catttttgat gactgagaat caatcttata taccttatac aataggtgtt gcaattcaat
      421 ttttatgggg gattttgctg ttatctttct ctattttgct attttggaag agaatagtac
      481 gataaaaatt ttgtattttg tgatattata taattatgaa agaaaaaaaa gtatatcaaa
      541 tattagcaat tgatgatgat gaaagcattc ttcgtttgat aaagaattct ttagaacgct
      601 cggagtttga agttacgacg aggagagaag taggtaacat agatatttgt tcattcattg
      661 gctttgattt aattttatta gatattatga tgtcgattga tggtttagaa atttgtaaat
      721 ccattcgaga acaaatcgat gtaccgataa tttttgtaac agccaagcaa ttggatagtg
      781 acttggctag tggagtaaaa gctggtgcgg atgattatat aaaaaaacca tttagtatta
      841 cagaattagt tgctcgtgtt aggatgcata tccaaaggga ggaaagaaat cgtaaaaata
      901 ataaagaaat atgcacaaag aatttgataa ttaatgtcag caatcaagaa atcttaattg
      961 aagaagagac tatatcattg acaaagcgag aatttacaat tttatatact ttagcaagca
     1021 acccgaagag agtatattcc gtagaggagc tctatgagag aatatatccg tcggaatcag
     1081 atgcacaatt tcgttctatt gctgagtata tttatcaaat tcgttcaaaa ttaaaaccat
     1141 attcaattaa tccgatcaag acacagtggg gaggcggata tagttggaac gaaaattaga
     1201 ttttaagact ttagtaaaaa agacgaagtg gacgattgtt aaacaatttt gtttgaatat
     1261 aattgtagct tatatttttc ctatatcaac tatttctatt gatattcatt cagaaactca
     1321 gtcaatcgct cgtgacttgt taggggtatt atttaatgtt ttttcatggg tttatatttg
     1381 tatatctttg attattatag tttctattaa tattaaagcc ttgttgacaa gagtaaagca
     1441 agaaatggag attgtttatc atagcagtat atggctaacc tctgtaaacg attcatctaa
     1501 tttaacaatt cttgaattca tagagacaaa tagacacatt gaatcaatgc aacaaagaat
     1561 aaaaaatatg attcaggtag agaaagacca aaaggcggat attctcttta aagtttcagc
     1621 gatggcacat gacttaaaaa cacctttgac tgtgattaaa ggaaactcgg aattgttgca
     1681 attggcacct ttatcaggta ttgatgttca atgtctcaag gatattgaaa gagcaagtaa
     1741 ccaattaaat agctatttta atcagttaat caactactct aaaacttttt actctgatca
     1801 gatttctttt catcagtatc gtatcagtaa tttatccaaa atactggaac aagagtgtac
     1861 ataccttatt ggagatagca ttgactacaa atatagtact aagatagacc gagactgtaa
     1921 tattgaattg gatttagatt taattatccg ttcggtggca aatattgtta ataatgctat
     1981 agcttatgca gatgatgcta aaaagaaaat cagagttacc tttgagttag cagatagcaa
     2041 tattacctta agtatttgga ataatggttc caaattttcg gatgatgttt tagaaaattt
     2101 tggaaaatta ttttatcgag aagatactag cagaaattca caatttgaac attttggaat
     2161 aggtttggca tttgtaaaac aagtaatgag cattcattca ggtgatatac aattgcaaaa
     2221 taaggaaaat ggagctatgg ttaaattaat tattccgatg aaaagtagtg actatgtcta
     2281 ggcttgaata tcgtaatttt gaatactatg aagttcagaa agtaagtata ttaataaaat
     2341 ttattttaaa atttcaacaa ttagcactca tacctgattt attcacattt gaatatatca
     2401 gatggggtgc aaaattatat tattttttaa aaacgcttac aaattgtaag ggtgggtaaa
     2461 aactcaattt caggagaaaa tgaagtgaat atttccacac taaaacgcat aatatcaagg
     2521 ttttttaggc tctataatat ttgtagtggg taaatccact atagatatta tggagcctat
     2581 tttattgtag aaaaaaaatc ccataagatc tataatgaaa agcgaccaaa caactcatta
     2641 gaaagattca tatgggacaa ttacatttta tcacaaaa
//
LOCUS       DAVKQL010000106         2578 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVKQL010000106 DAVKQL010000000
VERSION     DAVKQL010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.106"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            141..1337
                     /gene="tuf"
                     /locus_tag="VW561_002046"
     CDS             141..1337
                     /gene="tuf"
                     /locus_tag="VW561_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU9944245.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(1446..2578)
                     /locus_tag="VW561_002047"
                     /pseudo
     CDS             complement(1446..2578)
                     /locus_tag="VW561_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          797 a          498 c          541 g          742 t
ORIGIN      
        1 aatttttacc aagaataatt cacaaaaacg ttgtaaaaca cttgcaattt agctgaaatt
       61 tgataaaata gtaaggaaag ttagactgta ttgcctactg tctatctata aaatatattt
      121 tattggaggc ttttactcaa atggcaaaag aaaaatacga tcgtagtaaa ccacacgtta
      181 acattggtac tatcggacac gttgaccacg gtaaaactac cctaactgca gctatcacaa
      241 ctgttttggc acgtcgcttg ccttcatcag ttaaccaacc taaagactat gcgtctatcg
      301 atgctgctcc agaagaacgc gaacgcggta tcactatcaa cactgcgcac gttgagtacg
      361 aaactgaaaa acgtcactac gctcacatcg acgctccagg acacgcggac tacgttaaaa
      421 acatgatcac tggtgctgct caaatggacg gagctatcct tgtagtagct tcaactgacg
      481 gaccaatgcc acaaactcgt gagcacatcc ttctttcacg tcaggttggt gttaaacacc
      541 ttatcgtctt catgaacaaa gttgacttgg ttgacgacga agaattgctt gaattggttg
      601 aaatggaaat ccgtgaccta ttgtcagaat acgacttccc aggtgacgat cttccagtta
      661 tccaaggttc agcacttaaa gctcttgaag gtgactctaa atacgaagac atcgttatgg
      721 aattgatgaa cacagttgat gagtatatcc cagaaccaga acgtgacact gacaaaccat
      781 tgcttcttcc agtcgaggac gtattctcaa tcactggacg tggtacagtt gcttcaggac
      841 gtatcgaccg tggtatcgtt aaagtcaacg acgaaatcga aatcgttggt atcaaagaag
      901 aaactcaaaa agcagttgtt actggtgttg aaatgttccg taaacaactt gacgaaggtc
      961 ttgctggaga taacgtaggt gtccttcttc gtggtgttca acgtgatgaa atcgaacgtg
     1021 gacaagttat cgctaaacca ggttcaatca acccacacac taaattcaaa ggtgaagtct
     1081 acatccttac taaagaagaa ggtggacgtc acactccatt cttcaacaac taccgtccac
     1141 aattctactt ccgtactact gacgttacag gttcaatcga acttccagca ggtactgaaa
     1201 tggtaatgcc tggtgataac gtgacaatcg acgttgagtt gattcatcca atcgccgtag
     1261 aacaaggtac tacattctct atccgtgagg gtggacgtac tgttggttca ggtatggtta
     1321 cagaaatcga agcttaattc gatttagttc ccagaagaac aattatttaa gtcagacact
     1381 aaaagaatct tgcttggcaa ggttcttttt tggttctttg tcaactgtag tgggttgaaa
     1441 aaaaactaag ctcgagaaag gacaaatttc gtcctttctt ttttgatgtt cagggcgatg
     1501 aaaatccgtt ttttgaagtt ttcaaagttc cgaaaaccaa aggcattgcg cttgataagt
     1561 ttgatgagat tattggtcgc ttccaatttg gcgtttgaat agggtagttg aagggcgttg
     1621 acgattttct ttttgtcctt tagaaaggtt ttaaagacag tctgaaaaag aggatgaacc
     1681 tgcttaagat tgtcctcaat gagtccgaaa aatttctccg gttccttatt ctgaaagtga
     1741 aacagcaaga gttgatagag ctgatagtgg tgtttcaagt cttccgaata actcaaaagc
     1801 ttgtttaaaa tctctttatt ggttaagtgc atacgaaaag taggacgata aaatcgctta
     1861 tcactcagtt tacgactatc ctgttttatg agcttccagt agcgcttgat agccttgtat
     1921 tcatgagact ttcgatgaaa ctgattcatg atttggacac gcacacgact catagtacgg
     1981 ctaagatgtt gtacaatgtg aaagcgatcc aacacgattt tagcattcgg gagtgaaaca
     2041 gtctgggaga ctgtttcagc ctgagcctag aaatttgaaa gcgaagctgt ttagctaagt
     2101 catagtaagg actaaacata tccatagtaa taattttgac gcgacatcgg acaactctat
     2161 cgtagcgaag aaagtgattt cgaatgatag cttgtgttct accctcaaga acagtgatga
     2221 tatcgagctt ttcaaaatct tgcgcaatga agctcatctt tccctttgta aaagcatact
     2281 catcccaaga cataatctca ggaagacgcg aaaaatcatg tttaaagtga aaatcattga
     2341 gcttacgaat aacagttgaa gttgagatgg aaagctgatg ggcaatatca gtcatagaaa
     2401 tcttttcaat taacttttga gcaatctttt ggttgatgat acgagggatt tggtgattct
     2461 tcttgacgat agaagtttca gcgaccatca tttttgaaca gtgatagcac ttgaatcgac
     2521 gctttctaag gagaattcta gtaggcatac cagttgtttc gaggtaaggg atcttaga
//
LOCUS       DAVKQL010000107         2507 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVKQL010000107 DAVKQL010000000
VERSION     DAVKQL010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.107"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            142..1149
                     /locus_tag="VW561_002048"
     CDS             142..1149
                     /locus_tag="VW561_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU9944246.1"
                     /translation="MTEKLINSKPNGVFALILIELTIILGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKHISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            1152..1352
                     /locus_tag="VW561_002049"
     CDS             1152..1352
                     /locus_tag="VW561_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9944247.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            1504..1686
                     /gene="rpmF"
                     /locus_tag="VW561_002050"
     CDS             1504..1686
                     /gene="rpmF"
                     /locus_tag="VW561_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEU9944248.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            1702..1851
                     /gene="rpmG"
                     /locus_tag="VW561_002051"
     CDS             1702..1851
                     /gene="rpmG"
                     /locus_tag="VW561_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9944249.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(2343..>2507)
                     /locus_tag="VW561_002052"
     CDS             complement(2343..>2507)
                     /locus_tag="VW561_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944250.1"
                     /translation="DKGLWYYLNESGSMATGWFTVSGKWYYTYNSGDLLVNTTTPDGY
                     RVNANGEWVG"
BASE COUNT          833 a          477 c          454 g          743 t
ORIGIN      
        1 agagtatttg agcacaatat aatctcacct tttaaaagaa attattgggc tgaaagtttt
       61 tagtgattat tttcaacttt ctattgactt ttaaaaacaa agtgatatca tttagatatc
      121 aaataatggg aggtcattct tatgactgaa aaactaatca attcaaaacc aaatggtgta
      181 ttcgcattga ttctcattga gttgacaatc atacttggta tctttatatt tataatgggt
      241 gttggttcgg aaaacatttt tggaattatt atcggacctt tactaatcgt aattgcaggt
      301 ctagctcatg ctggtttaaa agttgtcaaa cctcaggaag ctctggttct gacactattt
      361 ggtaattata caggtaccat caaagaacct ggtttttact ttgtcaatcc cttcagcgta
      421 gcagtcaacc ctgcaaacca cactcgactt ggacaaagtg gtgatgttag cacaaaatct
      481 ccttttttag gagctaaatc atcaaatgac aatgatgtaa atcttgaaat tggcaagaaa
      541 cacatttccc tcaaagtcat gaccttgagc aattctcgtc aaaaaatcaa tgattgctta
      601 ggaaaccctg tagaaatcgg tatcgcggta acttggagag ttgtcgatac cgctaaggca
      661 gtcttcaacg ttgataacta caaagaatat ctttcattgc aatgtgatag cgccctccgt
      721 aatattgtcc gcatctatcc ttacgatgtg tctcctaatg tggatactac gggtgacggg
      781 caagcagatg aaggtagtct ccgtggctct agcgaaattg ttgctaaccg tattcgtgaa
      841 gaaatccaaa gtcgtgttga ggatgctggc ttggaaatcc ttgaagcacg tatcacttac
      901 ctagcttatg ctccagaaat tgctgccgtt atgcttcaac gccaacaagc atctgccatt
      961 attgatgcac ggaagatgat tgtagatggt gctgtaggaa tggttgaaat ggcactagaa
     1021 cggctcaatg aaggggaatt ggtagaactt gacgaagaac gaaaagctgc catggtttca
     1081 aatctccttg tcgttctttg tggcaatcat gatgcacaac caattgtcaa cacaggaagt
     1141 ctttactaaa gatggctgaa gctaaaaaaa agcagatccc ccttcgactc tcaacaaagt
     1201 tatacgctgc actcgcatca tgggcagaag acgactttcg ttcagtcaat gggcaaatcg
     1261 aatatctcct cacagagtgt gtcaagcaac ggaaaaaaga cggaaaatac gtatcagaaa
     1321 ccattgacga accatttgag attgatattt aacatcttct cctgtctgac aagtcggaca
     1381 ggagaatttt ttcatctttt tttgtcaagt aactttactt tacaaaaaaa atgtgttatc
     1441 ctagtatggt tgatgaaaat cagtagattg aatcgaatat aaatacctaa aggagaaatc
     1501 aaaatggcag tacctgcacg tcgcacttca aaagcgaaga aaaacaaacg tcgtacacac
     1561 tacaaagtaa cagctccatc tgtaaacttt gacgaaacta ctggagatta ctcacgttct
     1621 caccgtgtat cacttaaagg atactacaaa ggacgtaaaa tcgctaaagc tgcatcagct
     1681 gaataataga agggagatac catgcgcgta aatattacac ttgaacacaa agaatctggt
     1741 gaacgcttgt accttacttc taaaaacaaa cgtaacactc cagaccgcct tcaattgaag
     1801 aaatactcac caaaacttcg caaacacgtt gtgtttacag aagtgaagta agcattcaat
     1861 acgaatcaat acagaagaaa aacgcttgat cagcgttttt tctttttgtt aatttcacta
     1921 tattgcaagg gaaatcattc gaatgactac ctttttcact accttttttg aaataagaat
     1981 acggattcgg acgattaaaa gtaagagaaa ctcaaatgta agttggggtt ccttttttgt
     2041 atatttttca aataacgcct ctacactatt tgtagcaaat tcaccaacta cagttgtatc
     2101 ttagttaaaa taagttagaa tatgtaagtg agtaccagat ataccaagac atcgtcacca
     2161 tctaaggtat attcaaaata caaaagttga ccaactagat ttctgaatat ccttatatat
     2221 ccattcttaa aattggttta aatagcgtag tcttttaaac tagttttgag aatccaaaaa
     2281 atcttcctac atatgtaaga agatttttta gttcagaatg attagattta gctaatggat
     2341 acctatccta cccactcacc gttagcattg actcgatagc catcgggtgt agtcgtgttt
     2401 actaataaat ctcctgaatt ataggtatag taccatttac cagaaactgt aaaccaacca
     2461 gtagccattg aacctgattc atttaagtaa taccataagc ctttgtc
//
LOCUS       DAVKQL010000108         2481 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVKQL010000108 DAVKQL010000000
VERSION     DAVKQL010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.108"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            <1..1941
                     /locus_tag="VW561_002053"
     CDS             <1..1941
                     /locus_tag="VW561_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9944251.1"
                     /translation="KYDSHSKTMIFDLYANINDIVDGLAFAGDMRLFVKDDNQIKAET
                     KIRMPEKNKETKAEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYL
                     LKDNIILRKGYALKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYAD
                     SRNVEDGRSTQAVLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGK
                     DDKEYTGEDKSNVEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRG
                     KISDKGGFNWELRVNESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKAN
                     GFPDKVTDMDGNVYLQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGK
                     SVVFNINDKRNNGFDGEIQEQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTT
                     VKEFILNKDTGEVSELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDG
                     WEISGFEGKKDAGYVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQ
                     LNESHRKEDLQREDHSQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQT
                     LLAAGIMFIVGIFLGLKKKNQD"
BASE COUNT          983 a          309 c          465 g          724 t
ORIGIN      
        1 aagtatgact cacattcaaa aacgatgata tttgatttat acgctaatat taatgatatt
       61 gtagatggat tagcttttgc tggagatatg agattatttg ttaaagatga caatcaaata
      121 aaagctgaaa ctaaaattag aatgcctgaa aaaaataagg aaactaaagc agaatatccc
      181 tatgtatcaa gttatgggaa tgtaatcgaa ttaggagaag gagacctttc aaaaaacaaa
      241 ccagacaatt taactaaaat ggaatctggt aaaatctatt ctgattcaga aaaacaacaa
      301 tatctgttaa aggataacat cattctaaga aaaggctatg cactaaaagt gactacctat
      361 aatcctggaa aaacggatat gttagaagga aatggagtct atagcaagga agatatagca
      421 aaaatacaaa aggccaatcc taatctaaga gtcctttcag aaacaacaat ttatgctgat
      481 agtagaaatg ttgaagatgg aagaagtacc caagctgtat taatgtcggc tttggacggc
      541 tttaacatta taaggtatca agtgtttaca tttaaaatga acgataaagg ggaagctatc
      601 gataaagacg gaaatcttgt gacagattct tctaaacttg tattatttgg taaggatgat
      661 aaagaataca ctggagagga taagtccaat gtagaagcta taaaagaaga tggctccatg
      721 ttatttattg ataccaaacc agtaaacctt tcaatggata agaactactt taatccatct
      781 aaatctaata aaatttatgt acgaaatcca gaattttatt taagaggtaa gatttctgat
      841 aagggtggtt ttaactggga attgagagtt aatgaatcgg ttgtagataa ttatttaatc
      901 tacggagatt tacacattga taacactaga gattttaata ttaagctgaa tgttaaagac
      961 ggtgacatca tggactgggg aatgaaagac tataaagcaa acggatttcc agataaggta
     1021 acagatatgg atggaaatgt ttatcttcaa actggctata gcgatttgaa tgctaaagca
     1081 gttggagtcc actatcagtt tttatatgat aatgttaaac ccgaagtaaa cattgatcct
     1141 aagggaaata ctagtatcga atatgctgat ggaaaatctg tagtctttaa catcaatgat
     1201 aaaagaaata atggattcga tggtgagatt caagaacaac atatttatgt aaatggaaaa
     1261 gaatatacat catttgatga tattaaacaa ataacagaca agacactaaa cattaagatt
     1321 gttgtaaaag attttgcaag aaatacaacc gtaaaagaat tcattttaaa taaagatacg
     1381 ggagaggtaa gtgaattaaa acctcatagg gtaactgtga ccattcaaaa tggaaaagaa
     1441 atgagttcaa cgatagtgtc ggaagaagat tttattttac ctgtttataa gggtgaatta
     1501 gaaaaaggat accaatttga tggttgggaa atttctggtt tcgaaggtaa aaaagacgct
     1561 ggctatgtta ttaatctatc aaaagatacc tttataaaac ctgtattcaa gaaaatagag
     1621 gagaaaaagg aggaagaaaa taaacctact tttgatgtat cgaaaaagaa agataaccca
     1681 caagtaaacc atagtcaatt aaatgaaagt cacagaaaag aggatttaca aagagaagat
     1741 cattcacaaa aatctgattc aactaaggat gttacagcta cagttcttga taaaaacaat
     1801 atcagtagta aatcaactac taacaatcct aataagttgc caaaaactgg aacagcaagc
     1861 ggagcccaga cactattagc tgccggaata atgtttatag taggaatttt tcttggattg
     1921 aagaaaaaaa atcaagatta agacaaaagc tatagaaaaa aatggtttat gtactgagat
     1981 tagatagtga ggtgatgaca tagttttgtg aaaacagcca tttataactc aattatttag
     2041 tttacttcac tattgatact atttggagtt attaatggac ttagtttaga taactaatga
     2101 attgattgaa agggttagta ttgacaatat tggtcatatt gattagaaaa tagagtctat
     2161 caaaatttaa aggctaatag aggtgatgag acaatttcaa ggaattaagt agcgtttggt
     2221 tgcttaattc ctttttattt ggaaggaagt ttttcgtaac cgcccaataa cgaagtctat
     2281 tggacggtta cgatatgatc actgacttcg tcagtcttat ctacaacctc aaaactgtgt
     2341 tttgagcaac ctgcggctag cttcctagtt tactctttga ttttcattga gtattagaac
     2401 agaaaaaatg cttggagtat ttgtttgtgt gtttattttt atataacaaa ctataaacaa
     2461 aacaaaaata taaaaaaaga g
//
LOCUS       DAVKQL010000109         2438 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVKQL010000109 DAVKQL010000000
VERSION     DAVKQL010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.109"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            1..881
                     /locus_tag="VW561_002054"
                     /pseudo
     CDS             1..881
                     /locus_tag="VW561_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(993..1442)
                     /locus_tag="VW561_002055"
     CDS             complement(993..1442)
                     /locus_tag="VW561_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9944252.1"
                     /translation="MELVEFKNGHVNTVHEHIAITKVKEFLNNHDIPKDKGGIHKIST
                     DFFYNIIDMETTTEENRPWESHKEYYDVHYLLNGEEIILYNFLSQMELSEYKVDDDWQ
                     QMNGTALFSIKLKKDMLLLLEPNDAHKTGLLVEEPLNIKKVVFKVKI"
     gene            complement(1455..1861)
                     /gene="cdd"
                     /locus_tag="VW561_002056"
                     /pseudo
     CDS             complement(1455..1861)
                     /gene="cdd"
                     /locus_tag="VW561_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221146.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
     gene            complement(1948..2403)
                     /locus_tag="VW561_002057"
     CDS             complement(1948..2403)
                     /locus_tag="VW561_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEU9944253.1"
                     /translation="MNDTNKNQYFVYEFDTSFVEMSYSQFFEGYCILFSKEPYPSLSD
                     MPMEFRKEFLMEMSILGDAMLEVLNAYRINYDILGNSTPVLHAHLFPRYMWEEEERRK
                     TVVHRYDSSVFSNPEFEFTIEKHGPIIKKLRSAIDRHYREYQQLLDDSN"
BASE COUNT          820 a          431 c          364 g          823 t
ORIGIN      
        1 tgtaaaaaaa tatgaggagt tcggactcga ctctctcctt caagaaacac gtggtggtcg
       61 taaccatgca tatatgacag ttgaggaaga gaaagccttt cttgcccgtc atttgaaggc
      121 tacagaggca ggagaatttg ttacaattga tgccttattt caggcttata aaaaggagtt
      181 aggtcgttcc tacacacgtg atgccttcta tcaactgttg aagcgccatg gttggcgaaa
      241 tattatgcca cgtccagaac atcctaagaa agcagacgct caaaccattg tcgcgtctaa
      301 aaataaaatc tcaattcaag aagaaaagaa agcgctttaa aaccagtaga cgttttcata
      361 aggttcgctt gatgtaccaa gatgaggctg gcttcggtag aatcagtaaa ctgggatctt
      421 gttggtctcc aataggagta ggtccacatg tccatagtca ctatatacga gaatttcact
      481 attgttatgg agctgttgat gcccatacag gcgaatcatt tttcttaata gctggtggat
      541 gtaatactga gtggatgaac gtctttttag aagagctttc acaagcttat ccagatgatt
      601 attttttact cgttatggac aatgctatat ggcataaatc aagtatctta aagattccga
      661 ctaatattgg ctttgcattt attcctccat acacaccaga gatgaacccc attgaacaag
      721 tgtggaaaga gattcgtaaa cgtggattta agaataaagc ctttcgaact tcggaagatg
      781 tcatgaatca acttcaagat gtcatacaag gactggagaa ggaggtgata aagtccatcg
      841 ttaatcggag atggactaga atgctttttg aaagcagatg agtgttacta ttctatttta
      901 acaagcttta aattaaatat ctataatttt agaactttag aatacaattt cttaaatatt
      961 ttaaaattct taatccccga aagtactttc agttaaattt ttaccttaaa aacaactttc
     1021 ttaatattaa gtggttcttc aaccaatagg cctgttttat gtgcatcgtt tggttccaac
     1081 aaaagtaaca tatctttctt taatttaata ctaaataaag ctgtcccatt catttgttgc
     1141 caatcatcat ctactttata ttcagacaat tccatttgag acaagaaatt atataaaatt
     1201 atttcttctc catttaacag ataatggaca tcataatatt ccttatgact ttcccacggt
     1261 ctattctctt cagttgtagt ttccatatca attatattgt aaaagaaatc agtagaaatt
     1321 ttatgtatgc cacctttatc ttttggtata tcatgattat ttagaaactc ttttactttc
     1381 gttattgcga tatgctcatg aaccgtatta acatgtccat ttttaaattc aactaattcc
     1441 atagttccct cctgttataa attttcttga ttaaaactaa ctggtagtag ttctttaagt
     1501 ttatatactt tataagtatc aacatcacta gccaatataa cttcaaatga atcagaatta
     1561 caaaattcta tcattacctg gcgacacact ccgcaaggtg ctataaaaga tgataagata
     1621 ccattttttc cacctacaat acatattctt gaaaacgaac gttctccttt aaatacagct
     1681 ttataaaaag atactcgttc tgcacagcaa gtaggagaaa aagcagcatt ttctatatta
     1741 catccagaaa aaatttttta tcttcagtta ctaacgcagc tccaacctta aaatcagaat
     1801 aaggagcata agctttggcc attgatttaa tagcctctga tataattgct ttataagtca
     1861 ttcctaaaat atatttttca aaaaattata tcatccacaa cctagttgta atatacaagg
     1921 tattaaatct aatcgaaaat gtggagttca gtttgaatca tctaataatt gttgatactc
     1981 tctataatgt ctatcaatag cagacctcag ttttttaata attggtccat gcttttctat
     2041 agtaaattca aactcaggat tgctaaaaac tgaagaatca tatctatgaa ctacagtttt
     2101 tcttctctct tcctcttccc acatgtatct cggaaataaa tgagcatgaa gtacaggtgt
     2161 tgaatttcct aagatatcat aattaattct ataggcattt aacacttcaa gcatcgcatc
     2221 acctaatata ctcatttcca ttaaaaattc ttttctaaat tccattggca tatcagataa
     2281 agaaggatag ggttcttttg aaaaaagaat acaatatcct tcaaaaaact gagaataaga
     2341 catttctaca aagctagtat caaattcata aacaaagtat tgatttttat tagtatcatt
     2401 catttaatat tcccttatta tttgttaatc agtaatcc
//
LOCUS       DAVKQL010000110         2430 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVKQL010000110 DAVKQL010000000
VERSION     DAVKQL010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.110"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            <1..2267
                     /gene="lanM"
                     /locus_tag="VW561_002058"
     CDS             <1..2267
                     /gene="lanM"
                     /locus_tag="VW561_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 2 lantipeptide synthetase LanM"
                     /protein_id="HEU9944254.1"
                     /translation="LGLRGKLNDIKFGKGDTHSQGKTVLILFFDDAKIVYKPKNLIIN
                     NSLNTIAEYIRKVDEKIRIRIPRTIAYSDHSYEEFIDYLPLEQKKKLPEYYYNFGVLL
                     AFIYLFNGSDIHFENLISYGDMPVIIDFETMLQQPLFDDKTGQSLLDTLFHRVTRTLL
                     LPTEGVKREDGLDVEMSALTGNFKKDAFNGQVLINLNTDKVKFDIGKIDFEGGKNLPV
                     RDGDIEFDKYIKDFKKGFRDFYLIFEELNKTEEFKMLLKANLYGLKTRVLFRDTNSYA
                     SVLSFLYHPDFYEEMLDREKALENLWSNKFSNQGIVASECEQMRLLDIPIFYTDTNIN
                     EIYDDFGNHFISLHQFSGYDYFMKYLPNNNLENLEKQLDIIEIYFPSFDQRKKGYKLN
                     LSNRKKLPNESLVTILHNEVENIADSLVINNIYDEYPIWSIPVNFKGSKWCLEQTGND
                     MYRGRAGILLFMYYVKLLKDKSSYTQLYNKMLSAIPPSVSLSKTTFGLTGDIGYFIVL
                     SIIEDYDTNTLACLNYIKSVLTELEKTDFASVSNKSDYINGLLPLINTLVRYYRRGIE
                     KERVLRLVLSLGDTLYNDVSERDNSNFGYSFGHGLSGVIFTLKNIYKVTRLKKYKELI
                     LQLVPREKIKIHSLKWCSGEFGTLVNHPQSVPEGIVASLDNDTFCHGSMAIINFYIDY
                     MKEMDTSMELESLLCSVVMNKSKEGEYRIVQTSNFPDFSLYTGVTGIAYTFLRYIALN
                     QNDKKIVIPSLLEI"
BASE COUNT          878 a          284 c          435 g          833 t
ORIGIN      
        1 aattagggct aagggggaaa ttaaatgata taaaatttgg aaagggtgat acacacagcc
       61 aaggaaaaac tgttttgata ctcttctttg atgacgcgaa aattgtttac aagcctaaaa
      121 atttaataat caataactca ctaaatacta ttgctgagta tatccgaaag gttgatgaaa
      181 aaattaggat aagaatacct cgaactattg cttattcgga tcacagctat gaagaattta
      241 ttgattatct acctctagag caaaagaaaa aattacctga atattattat aattttggtg
      301 tgcttttagc atttatatat ttatttaatg ggagtgatat acattttgaa aatttaattt
      361 cctatggaga tatgcctgta ataatagact ttgaaacaat gttacagcaa cctctatttg
      421 atgataaaac tggtcagagt ttgctggata ccctgtttca cagggtgact cgaactttgc
      481 ttcttccaac agagggagta aaaagggagg atggtcttga tgttgagatg agtgctctta
      541 caggaaattt taagaaggat gcttttaatg gtcaagtttt aattaattta aatactgata
      601 aggtaaagtt tgatattggg aaaattgatt ttgaaggtgg gaaaaacctg cctgttcggg
      661 atggtgatat agaatttgat aagtatataa aagatttcaa aaaaggtttt agggattttt
      721 atctgatatt tgaagagtta aataaaacag aagaatttaa aatgctatta aaagcaaact
      781 tgtatggttt aaagacaagg gtacttttta gagacacaaa tagctatgct tcagtactat
      841 cttttttata tcatcctgat ttttatgaag aaatgttaga tagagaaaaa gcattggaaa
      901 atctctggag caataagttt tctaatcaag gtattgtagc aagcgaatgt gaacaaatga
      961 ggttattaga tattccgatt ttttatacag atacaaatat caatgaaatc tacgatgatt
     1021 ttggaaatca ttttatttcc ctacatcaat tttctggata tgactatttt atgaaatatt
     1081 tacctaataa taatttggaa aatttagaaa agcaacttga tatcattgaa atttattttc
     1141 caagctttga tcagcgaaaa aaaggatata aattaaactt atctaataga aaaaaactcc
     1201 caaatgaaag tttagttact attctgcata atgaagtaga aaatattgca gactctttgg
     1261 ttattaataa tatttatgac gaatatccaa tttggagtat acctgttaat tttaaaggtt
     1321 ctaaatggtg tcttgaacaa acgggtaatg atatgtatag gggtagagct ggaatattac
     1381 tgtttatgta ctatgtaaaa ttattgaaag ataaatcatc atatactcaa ctgtacaata
     1441 aaatgttatc tgcaattccg ccatctgttt ctttatctaa gaccacattt ggtttaactg
     1501 gggatatagg atatttcatt gttctatcca ttatagagga ttacgataca aatactctcg
     1561 catgcttaaa ttatataaag agtgttttaa ctgagcttga aaagactgat tttgcaagtg
     1621 tttcaaacaa gtctgactat attaatggtc ttctaccttt aattaataca ctagtaagat
     1681 attataggag aggaatagag aaggaacgtg ttctaaggct agtattaagt ttaggtgata
     1741 cgctatataa tgatgtaagc gagagagata atagtaattt tggatatagt tttggccatg
     1801 gactatcagg tgtaattttt acattaaaaa atatttataa agtgacaaga cttaaaaaat
     1861 ataaagaatt aattttacaa ctagttccta gggagaaaat caaaattcat tctttgaagt
     1921 ggtgtagtgg agaatttggg acattagtta atcatcccca aagtgttcca gagggaattg
     1981 tagctagttt agataatgat acattttgcc atggaagtat ggccataata aatttttaca
     2041 tcgactacat gaaagaaatg gatactagta tggaattgga gtctttattg tgtagtgttg
     2101 taatgaataa atcaaaagaa ggagagtatc gtatcgtcca aacaagcaat ttcccagatt
     2161 tttctttgta tacaggtgtg actggtattg cttatacttt tttaaggtat atcgcactga
     2221 atcagaatga caaaaagata gttatcccct cattacttga aatctagctt aatttatagt
     2281 tttgtttcta aaatagaggt gtccactcat taagcaatat aaaaatacca agattgacta
     2341 gtttaatatg taattctata aaatattttt aatagtgtag cttttcgttg agataataat
     2401 ataataaaga aaggttctat tctaattgct
//
LOCUS       DAVKQL010000111         2330 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVKQL010000111 DAVKQL010000000
VERSION     DAVKQL010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.111"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            33..905
                     /locus_tag="VW561_002059"
     CDS             33..905
                     /locus_tag="VW561_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9944255.1"
                     /translation="MIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQT
                     MLRNPLASPDIIGVTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKD
                     GFSAARLIILGIGFQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLI
                     VSIIATILVLFFNKRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPI
                     ACISFLAGPIALNIGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSP
                     YLIYLLIKMNRRNI"
     gene            905..1699
                     /locus_tag="VW561_002060"
     CDS             905..1699
                     /locus_tag="VW561_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9944256.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVSS"
     gene            2267..>2330
                     /locus_tag="VW561_002061"
     CDS             2267..>2330
                     /locus_tag="VW561_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEU9944257.1"
                     /translation="MKIHCLKLKNKELNKEVAFYL"
BASE COUNT          871 a          344 c          384 g          731 t
ORIGIN      
        1 ttatcggaga tgaaagttat tctttttcaa ctttgattaa agtcttaaat agtgaaactg
       61 ttcctggagc tagtttttcg attatggaaa ttagattacc aaaattatta gcaggaatta
      121 tagctggctg gtcttttgga ttggcaggat ttatctttca aactatgtta agaaatcctc
      181 ttgcaagtcc tgatataatc ggtgtcacaa gttcttcatc tattgcagcg gtcttttgca
      241 tattggtatt aaaaacaaat agtttaacta ctggaattat ttcaataact tgtggactaa
      301 catcatcttt aatattattt ttactagcta aaaaagatgg tttttcagca gcaagactga
      361 taatattagg tattggtttt caagctgtca caagagcagg cacctcattt ttattgttga
      421 aagtagcaag atatgaatta caagaagtta tgagatggct cagtggctct ttatctttta
      481 caaagttaga tgacatacct cttgttctaa tagtaagtat tattgctact atattagttt
      541 tattttttaa taaaagacta gaaattattg aacttggtga agaaatagca atcggacttg
      601 gagcaaatcc cgagctttca aggcttgttt taattttttg cgctgtatct ttaactgctt
      661 tttctacttc aattacagga ccaatagctt gtatatcttt tttagctggt cccatagcct
      721 taaatattgg caagaaaaga agcccaatat tagctggatt ggttggaatt ttactagttt
      781 tgttatcaga catattctct caaaatattt taccagctag atatccagta ggtgttgtaa
      841 ctggcttgtt aggttcacca tacttaatat acttactaat aaaaatgaac aggaggaata
      901 tataatgaaa ggtctgtggt caaataatct tacctgcggt tatgatgaaa aaataatctt
      961 ggaaaatata aatataaaaa tacctgaaga aaaaatatca gttattattg ggtcaaatgg
     1021 ttgtgggaaa tcaacactca ttaaaacctt gtctcgactt ataaagccat tagagggaga
     1081 agtattgctc gataataaat caattaattc ttataaagaa aaagatttag caaaacacat
     1141 agctatatta cctcaatctc caataatccc tgaatcaata acagtagctg atcttgtaag
     1201 ccgtggtcgt ttcccctaca gaaagccttt taagagtctt ggaaaagatg accttgaaat
     1261 aataaacaga tcaatggtta aggccaatgt tgaagatcta gcaaataacc tagttgaaga
     1321 actttctggg ggtcaaaggc aaagagtatg gatagctcta gccctagccc aagatacaag
     1381 tatcctactt ttagatgagc caactactta cttggatatc tcatatcaaa tagaactatt
     1441 agacctcttg actgatctaa accaaaaata taagacaacc atttgcatga ttttgcacga
     1501 tataaatcta acagcaagat acgctgatta cctatttgca attaaagaag gtaaacttgt
     1561 tgcagaggga aagcctgaag atatactaaa tgataaacta gttaaagata tctttaatct
     1621 tgaagcaaaa attatacgtg accctatttc caattcgcct ctaatgattc ctattggcaa
     1681 gcaccatgtt agctcttaat aattgaatat cggaattcat ttaaaagtcg caaattttga
     1741 actgaccctc aaaagttagg ccaaaaaact aactaaagga ggtcagtttt ttaatggcaa
     1801 atacagcaca gaatttaaga taaaattagt aaaagaatat catgaatgaa aaataggata
     1861 tagagaataa cagcaatatt aaaaagatca ggactgataa taaacaaaaa gaaagtacaa
     1921 cgactagttc aaaagctaaa acttcaagta aaaagttatt caagaaaatc tagaaaatac
     1981 tcatcctaca aaggacaggt aggaaaaata tcggacaaca aaataaaaag aaatatttat
     2041 taccgaaaaa aaggggaaat cgagtcaagt accaacaggc tcatctattc caaaaagata
     2101 tttactcgat ggaaaaactc ctagaattag tgctacaaat attgacaatg gtatcttagg
     2161 ttactacgaa gatatagatg ataaaaatta tagagttttt gaaaatttta tatctgtttc
     2221 atttttagga gcagtctttt atcataaata taaagcaagc ctagatatga aaattcattg
     2281 tttaaaatta aaaaataaag aattgaataa agaagtagca ttctatttaa
//
LOCUS       DAVKQL010000112         2196 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVKQL010000112 DAVKQL010000000
VERSION     DAVKQL010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.112"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            <1..748
                     /locus_tag="VW561_002062"
     CDS             <1..748
                     /locus_tag="VW561_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9944258.1"
                     /translation="IPERVSQDIKFDKKIELKDIWYKYGLFDDYVLKGINVTIKKGET
                     VAIVGESGSGKSTLAKILLGLLEPNIGSIEVDGVEKEEIGQTLYRKIFGAVLQNSTLS
                     YGTLRENLTFGHFVSDEELMTNLNSIGLSNVVKSLPLGLETIIAEEGNNFSGGQQQMI
                     LLARCLLSKPSVVVLDEATSSLDNLSQQITTSYLSEIGTTKILIAHRLDTIKSADKIL
                     VMHNGEIVEIGTHRELLELGGIYKQLYSNN"
     gene            772..>2196
                     /locus_tag="VW561_002063"
     CDS             772..>2196
                     /locus_tag="VW561_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU9944259.1"
                     /translation="MKIMKKKYWTLAILFFCLFNNSVTAQEIPKNLDGNITHTQTSES
                     FSESDEKQVDYSNKNQEEVDQNKFRIQIDKTELFVTTDKHLEKNCCKLELEPQINNDI
                     VNSESNNLLGEDNLDNKIKENVSHLDNRGGNIEHDKDNLESSIVRKYEWDIDKVTGGG
                     ESYKLYSKSNSKVSIAILDSGVDLQNTGLLKNLSNHSKNYVPNKGYLGKEEGEEGIIS
                     DIQDRLGHGTAVVAQIVGDDNINGVNPHVNINVYRIFGKSSASPDWIVKAIFDAVDDG
                     NDIINLSTGQYLMIDGEYEDGTNDFETFLKYKKAIDYANQKGVIIVAALGNDSLNVSN
                     QSDLLKLISSRKKVRKPGLVVDVPSYFSSTISVGGIDRLGNLSDFSNKGDSDAIYAPA
                     GSTLSLSELGLNNFINAEKYKEDWIFSATLGGYTYLYGNSFAAPKVSGAIAMIIDKYK
                     LKDQPYNYMFVKKILEETLPVKNGI"
BASE COUNT          836 a          261 c          408 g          691 t
ORIGIN      
        1 aattccagaa agagtcagtc aagatataaa atttgataaa aaaatagaat taaaagatat
       61 ttggtataaa tatggattat ttgatgatta tgttttgaaa ggaataaatg ttactattaa
      121 aaaaggagaa actgttgcta ttgttggaga atcaggttca ggtaagagta cattagctaa
      181 aattttatta ggtttattag aacctaatat tggttcaata gaagttgatg gagtagaaaa
      241 agaagaaatt ggtcaaacat tgtatagaaa gatttttgga gcagtgttac aaaattcaac
      301 cctaagttat ggtaccttaa gagagaattt gacatttgga cactttgttt cagatgaaga
      361 attaatgaca aatctaaatt caattggtct tagcaatgta gttaaatctt tacctcttgg
      421 attagagaca atcatcgctg aagaaggtaa taacttttct ggagggcagc agcaaatgat
      481 acttttagct cgttgtcttt tgtcgaaacc ttcggtagtt gttttggacg aagcaacaag
      541 tagtttagat aatttatctc aacaaattac aacttcttac ttaagtgaaa tcggtaccac
      601 taagatttta attgcccatc gactagatac tatcaagtct gcagataaga tcttagtaat
      661 gcataatggt gaaattgtag agattgggac ccatagagaa cttcttgaac taggaggcat
      721 ttataagcaa ttgtattcaa ataattagtt tttgattaaa agggtaaatt tatgaagatt
      781 atgaaaaaaa aatattggac tttagcgata ttattctttt gtttgttcaa taattctgtt
      841 actgctcaag aaatacctaa aaatcttgat ggcaatataa ctcacactca gactagcgaa
      901 agtttttctg aatctgatga aaaacaggtt gactattcta ataaaaatca agaagaagta
      961 gaccaaaata aatttcgtat tcaaatcgat aagacagaat tatttgtaac aacagataaa
     1021 catttagaaa aaaactgttg taaattggaa cttgaaccac aaataaataa cgatattgtt
     1081 aactctgaaa gtaataattt actaggcgaa gataatttag ataataaaat taaggaaaat
     1141 gtttctcatc tagataatag aggaggaaat atagagcatg acaaagataa cttagaatcg
     1201 tcgattgtaa gaaaatatga atgggatata gataaagtta ctggtggagg cgaaagttat
     1261 aaattatatt ctaaaagtaa ttctaaagtt tcaattgcta ttttagattc aggagtcgat
     1321 ttacaaaata ctggattact gaaaaatctt tcaaatcact caaaaaacta tgtccccaat
     1381 aaaggatatt taggaaaaga ggagggagag gaaggaataa tatcagatat tcaagataga
     1441 ttaggtcatg gtacggctgt tgtagctcaa attgtagggg atgacaatat taatggagta
     1501 aatcctcacg ttaatattaa cgtctataga atatttggta agtcgtcagc tagtccagat
     1561 tggattgtaa aagcaatttt tgatgctgta gatgatggca atgatattat caatcttagt
     1621 actggacaat atttaatgat tgatggagaa tatgaggacg gaacaaatga ttttgaaaca
     1681 tttttgaagt ataaaaaggc tattgattac gcgaatcaaa aaggagtaat tatagtagct
     1741 gcattaggga atgactccct aaatgtatca aatcagtcag atttattgaa acttattagt
     1801 tcacgcaaaa aagtaagaaa accaggatta gtagttgatg ttccaagtta tttctcatct
     1861 acaatttcgg tcggaggcat agatcgctta ggtaatttat cagattttag caataaaggg
     1921 gattctgatg caatatatgc gcctgcaggc tcaacattat ctctttcaga attaggactt
     1981 aataacttta ttaatgcaga aaaatataaa gaagattgga ttttttcggc aacactagga
     2041 ggatatacgt atctttatgg aaactcattt gctgctccta aagtttctgg tgcgattgca
     2101 atgattattg ataaatacaa attaaaagat cagccctata attatatgtt tgtaaaaaaa
     2161 attctggaag aaacattacc agtaaaaaat ggtata
//
LOCUS       DAVKQL010000113         2189 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVKQL010000113 DAVKQL010000000
VERSION     DAVKQL010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.113"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            103..1128
                     /locus_tag="VW561_002064"
     CDS             103..1128
                     /locus_tag="VW561_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU9944260.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
     gene            1118..2125
                     /locus_tag="VW561_002065"
     CDS             1118..2125
                     /locus_tag="VW561_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9944261.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
BASE COUNT          778 a          306 c          379 g          726 t
ORIGIN      
        1 tccttttttg aaaaaattat attattaaat ttgtaactgt atctattgac aatgataatt
       61 attatcgata caatagactt gaaatatgtt taaggagttt ttatgaaaaa caaatttttt
      121 ctaatagcta ttttagctat gtgtatagtt tttagcgctt gttcttctaa ttctgttaaa
      181 aatgaagaaa atacttctaa agagcatgcg cctgataaaa tagttttaga tcatgctttc
      241 ggtcaaacta tattagataa aaaacctgaa agagttgcaa ctattgcttg gggaaatcat
      301 gatgtagcat tagctttagg aatagttcct gttggatttt caaaagcaaa ttacggtgta
      361 agtgctgata aaggagtttt accatggaca gaagaaaaaa tcaaagaact aaatggtaaa
      421 gctaacctat ttgacgattt ggatggactt aactttgaag caatatcaaa ttctaaacca
      481 gatgttatct tagcaggtta ttctggtata actaaagaag attatgacac tctatcaaaa
      541 attgctcctg tagcagcata caaatctaaa ccttggcaaa ctttatggag agatatgatt
      601 aaaattgatt caaaagcctt aggtatggaa aaagaaggtg atgagttaat caaaaatact
      661 gaagctcgta tatccaaaga attagaaaaa catccagaaa tcaaaggaaa aatcaaagga
      721 aaaaaagtat tatttactat gattaatgct gcagatacat caaaattctg gatttatact
      781 agcaaagatc caagagcaaa ttatttaaca gatttaggtc tagttttccc tgaatcatta
      841 aaagaatttg agagtgaaga tagttttgca aaggaaattt ctgcagaaga agcaaataag
      901 ataaatgatg ctgatgtaat cataacttat ggtgatgata aaactcttga agctttacaa
      961 aaagatcctc ttttaggtaa aataaatgca attaaaaatg gtgccgttgc tgtaattcca
     1021 gataatacac cgttagcagc ctcatgcact ccaacaccac tttcaataaa ctatactatt
     1081 gaagaatacc taaatctttt aggaaatgca tgcaaaaatg cgaaataaaa aacaaataaa
     1141 cctaggcata atttttgtaa tctgcctagg tcttcttatt acaatatttt tgtcattaaa
     1201 gcttggaaca aaagaaatta atatcagaga ttttttagca gcttttggaa tgggcaatac
     1261 aaatgatgat tttattaaat caattatata taaaagaata cctagaacta tttttgcaat
     1321 tttagcaggt tctagtcttg ccataagcgg tgtattgatg caatcagtta ctagaaaccc
     1381 aatagctgat ccaggtatac tcggtataaa cacaggagca agtcttagtg tagtaattgg
     1441 tctttctttt ttaggaattt catcaagcat aagccatata agttttgcaa tcattggtgg
     1501 cttagtaagt gcaatttttg tatacgcgat tgctgtaagc ggaaaagcag gccttacccc
     1561 tataaaactt gccttatcag gaacttgtgt tagtatggct ttaagcagtt ttgtaagttt
     1621 tttaatttta ccgaataata acgtcttaga caaatttaga ttttggcaaa taggtagcct
     1681 tggagcagct acattatctt ctatatctac actactacct tttataattt taggtcactt
     1741 gatagctata tttatttcat cagatttaaa cgctttagct atgggtgatg aaatggctgt
     1801 tggtcttgga gttaatgtta ataggataag atcacttgca ataattgcaa gtgtgctttt
     1861 atgttcaagt attactgcaa ttggtggacc tattggcttc gtaggtctta tagttcctca
     1921 cttttgtggc ttatttataa gcaaagatat acgcacaatg accatttctt catcttttat
     1981 aggtgcagag ctcttgctta tatgtgatat aatcggccgt atgttaggta aaccaggtga
     2041 aattgaagta gggataatta ctgcaataat cgggggtcca gtacttattt atgtaactat
     2101 gaaaaataga ggggttaata actaatgcaa aatttaatta taggtattca aaaaagaaaa
     2161 aatagaataa cactattttc ctcactatt
//
LOCUS       DAVKQL010000114         2153 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVKQL010000114 DAVKQL010000000
VERSION     DAVKQL010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.114"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            <1..1094
                     /locus_tag="VW561_002066"
     CDS             <1..1094
                     /locus_tag="VW561_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU9944262.1"
                     /translation="QKPSKIPYLETTGMPTRILLRKRRFKCYHCSKMMVAETSIVKKN
                     HQIPRIINQKIAQKLIEKISMTDIAHQLSISTSTVIRKLNDFHFKHDFSCLPEIMSWD
                     EYAFTKGKMSFIAQDFNNLNIITVLEGRTQAIIRNHFLKYDRAVRCRVKIITMDMFSP
                     YYDLARQLFPCAKIVLDRFHIVQHLSRAMSRVRVQIMNQLDRKSHEYKAIKRYWKLIQ
                     QDSRKLSDKHFYRPTFRMHLTNKEILNKLLSYSQDLKHHYQLYQLLLFHFQNKEPEKF
                     FGLIEDNLKQVHPLFQTVFKTFLKDKEKIVNALQLHYSNAKLEATNNLIKLIKRNAFG
                     FRNFENFKKRIFIALNIKKERTKFVLSRA"
     gene            complement(1298..>2153)
                     /locus_tag="VW561_002067"
     CDS             complement(1298..>2153)
                     /locus_tag="VW561_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choline-binding protein A"
                     /protein_id="HEU9944263.1"
                     /translation="RLEKIKTDRKKAEEEAKRKAAEEDKVKEKPAEQPQPAPAPQPEK
                     PAEEPNPAPAPKPEKPADQPKAEKPADQQAEEDYARRSEEEYNRLTQQQPPKPEQPAP
                     APKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNSNGAMATGWLQYNGSWYYLNA
                     NGDMATGWFQYNGSWYYLNANGDMATGWLQYNGSWYYLNSNGAMVTGWLQNNGSWYYL
                     NANGSMATDWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGSGALAVNTTVDSYRV
                     NANGEWVN"
BASE COUNT          583 a          455 c          396 g          719 t
ORIGIN      
        1 ttcaaaaacc ttctaaaatt ccttatcttg aaacgactgg tatgcctact agaattctcc
       61 ttagaaagcg tcgattcaag tgctatcact gttcaaaaat gatggtcgct gaaacttcta
      121 tcgtcaagaa gaatcaccaa atccctcgta tcatcaacca aaagattgct caaaagttaa
      181 ttgaaaagat ttctatgact gatattgccc atcagctttc catctcaact tcaactgtta
      241 ttcgtaagct caatgacttt cactttaaac atgatttttc ttgtcttcct gagattatgt
      301 cttgggatga gtatgctttt acaaagggaa agatgagctt cattgcgcaa gattttaaca
      361 atctcaatat catcactgtt cttgagggaa gaacacaagc tatcattcga aatcactttc
      421 ttaaatatga tagagccgtc cgatgtcgcg tcaaaattat tactatggat atgtttagtc
      481 cttactatga cttagctaga caacttttcc catgtgctaa aatcgtgttg gatcgctttc
      541 acattgtaca acatcttagc cgtgctatga gtcgtgtgcg tgttcaaatc atgaatcaat
      601 tggatcgaaa gtctcatgaa tacaaggcta tcaagcgcta ctggaagctc atacaacagg
      661 atagtcgtaa actcagcgat aaacattttt atcgccctac ttttcgtatg catttaacca
      721 ataaagagat tttaaacaag cttttgagct attcacaaga cttgaaacat cactatcagc
      781 tctatcaact cttgctgttt cactttcaga ataaggaacc ggagaaattt ttcggactca
      841 ttgaggacaa tctaaagcag gttcatcctc tttttcagac tgtctttaaa acctttctaa
      901 aggacaaaga gaaaatcgtc aacgcccttc aactacacta ttctaacgcc aaattggaag
      961 cgaccaataa tctcatcaaa cttatcaagc gcaatgcctt tggttttcgg aactttgaaa
     1021 acttcaaaaa acggattttt atcgctctga atatcaaaaa agaaaggaca aaatttgtcc
     1081 tttctcgagc ttagctgact tcaacccact acagttgaca aagagccaaa aaaaactccg
     1141 tagtctctag cgctataaat ccggctagag attacagagt tattttttaa tctaaacttc
     1201 ctacaagggt atattatcta ctgaaaggat atggtatttg tagggaatac tttaaagagt
     1261 tcttacagga agtcagtatt aactagttat attaagttta gtttacccat tcaccattgg
     1321 cattgactct atagctatct acagttgtgt tgactgcaag ggcacctgag ccattgacat
     1381 agtaccattt atctgatact ttgaaccatt ggcttgcttt catagcacct gatgcttcaa
     1441 gatagtacca ggtatctcca tctttcaccc aatctgttgc cattgaaccg ttagcgttta
     1501 ggtagtacca tgagccattg ttttggagcc atcctgttac catagcacca ttgctgttta
     1561 ggtagtacca tgaaccattg tattggagcc atcctgtcgc catatcacca ttagcgttga
     1621 ggtagtacca tgaaccattg tattggaacc atcctgtcgc catatcacca ttagcgttga
     1681 ggtagtacca tgaaccattg tattggagcc atcctgtcgc catagcgcca ttgctgttga
     1741 ggtagtacca tgagccattg ttttggagcc atcctgtcgc cattgaacca tcagtattgt
     1801 agaagtacca cataccgttt tcttgtttcc agcctgtttt tggtgcagga gctggttgct
     1861 ctggttttgg cggttgctgt tgagtcaagc gattatattc ttcttctgat ctacgagcat
     1921 agtcttcttc agcttgttga tcagctggtt tttctgcttt tggttgatca gctggcttct
     1981 ctggttttgg agctggagct ggattaggct cttcagctgg tttttctggt tgaggagccg
     2041 gcgctggttg tggttgttca gctggttttt ctttaacttt atcttcttct gctgcttttc
     2101 gtttagcttc ttcttctgct tttttacgat ctgtcttgat tttttctaac ctt
//
LOCUS       DAVKQL010000115         2050 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVKQL010000115 DAVKQL010000000
VERSION     DAVKQL010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.115"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(35..496)
                     /locus_tag="VW561_002068"
     CDS             complement(35..496)
                     /locus_tag="VW561_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9944264.1"
                     /translation="MGKERRKQGNLERKKRVVKKRKIPLEELKAFVEAHPDAFLREIA
                     ARFDCALPSVWAVLKQIKVILKKTTSFREQKPEKVSEFLDILDNLKDLPIVYIDEMGI
                     NRYLYRPYAGAPRGEKVYDKISGRRFERTNEVEQKLNGSFLIRYIDSQIRE"
     gene            complement(463..564)
                     /locus_tag="VW561_002069"
     CDS             complement(463..564)
                     /locus_tag="VW561_002069"
                     /inference="COORDINATES: protein motif:HMM:NF013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944265.1"
                     /translation="MKGGHRHVEAAKVFDVGARTLFTWEKKDVNKGT"
     gene            873..1253
                     /locus_tag="VW561_002070"
     CDS             873..1253
                     /locus_tag="VW561_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9944266.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            1352..1573
                     /locus_tag="VW561_002071"
     CDS             1352..1573
                     /locus_tag="VW561_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU9944267.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            1590..1904
                     /gene="trxA"
                     /locus_tag="VW561_002072"
     CDS             1590..1904
                     /gene="trxA"
                     /locus_tag="VW561_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU9944268.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
BASE COUNT          648 a          414 c          378 g          610 t
ORIGIN      
        1 gtttatattc ctatttttat gcataattat tctgtcattc tctaatttgt gaatcaatat
       61 atctgattag aaaactacca ttgagttttt gctccacctc attagtccgc tcaaaacgcc
      121 gtccgctaat cttgtcatag actttctccc ctctaggagc ccctgcataa ggacgataga
      181 ggtagcggtt gattcccatt tcgtcaatat atacgattgg taaatctttt aggttatcca
      241 aaatatcaag aaactcagaa actttctcag gcttttgttc cctaaaactg gtcgtctttt
      301 ttaaaatgac cttaatctgc tttaaaactg cccatacgga gggcaaagca caatcaaaac
      361 gggccgcaat ttcccgtaaa aaagcgtctg gatgagcctc tacaaaggct ttcaattctt
      421 ctaaagggat cttacgcttt ttgacgactc gctttttccg ctctaagttc ccttgtttac
      481 gtctttcttt tcccacgtga agagagttct ggcgccgaca tcaaaaactt tggccgcctc
      541 gacatgtctg tgccctcctt taatggaatc taatgctcct tgtttaaaat cagtactata
      601 tgcatagctt tattagacca tgcttatttt aaactaagca actatagata gagaaatgaa
      661 ataaaatctg aattcgaatg tgataaggag ttagaaattg aaacaatagg attcccccat
      721 tattcacaca aaataaaacc actaaaaaac agagcttgcc agcattcttg aaaagtagta
      781 taattattgc atgcgcaatc atcttgtgac gcagagactg cccgttaatg gactttcttc
      841 tatattatga ctctaaaaag aaaggagaca ctatgaccta tttggaaaaa tggtttgact
      901 tcaatcggcg tcagaaagaa attgaaagtc tcttggaaga gaccattgcc caacagagcg
      961 agcaaagtct gaccttgaaa gagttctacc tgctctacta tctggacttg gctgaagaaa
     1021 aatctctacg ccagattgac ctgccagata agcttcatct gagcccgagc gcagtttcta
     1081 ggatggtggc tcgcttggaa gctaaaaatt gcggtctact cagtcgcatg tgttgccatc
     1141 aagatagacg ctctagtttt atctgcctga caaatgatgg gcaaaagaca ctggcctcgc
     1201 tacaaaagac tgtcgaagaa agcttggaag caggtttgga tttcttgatt taagatgatt
     1261 ttaaaaaaag ttgcgtgcgc aatcattttt cttgacattc tcttttataa ggagtaaaat
     1321 aaagttagca ttaaacaaag gagttttaaa tatgattgaa attacctata tagatgccag
     1381 caagaacgaa agaactgtga ctttcgagtc ttatgaagac tttgaacgtt cgcaacaagc
     1441 ttgccttatc ggcgtcgcag actactaccc tgtccaaaaa ttaacttaca agggtcataa
     1501 tttggactac catgggactt atggagatat cttcttctat ctcatgaaac aagatttaag
     1561 ccaatataac taaaaaaagg agaaatacaa tggcaaaagc aattacagat gcaacattcg
     1621 aacaagaaac aaaagacggt ttggtcttag tagacttctg ggcaacttgg tgtggtccat
     1681 gtcgtatgca aggtccaatc ttggacaaat tgtctgaaga actttcagaa gatgtcttga
     1741 aaatcgttaa aatggacgtt gatgaaaatc caaacacagc tcgtgctttt ggaatcatgt
     1801 ctattccaac tcttctcttc aaaaaagacg gccaagttgt caaacaagtt gcaggtgttc
     1861 acacagcaga acaaatcaag gccatcattg ctgaattgag ctaatcaaac gagagaccaa
     1921 gtactttctt tggtctcttt tttcttgccc tttgccattt ttcaaaaaat atgctagact
     1981 atagtagatt gaaactagaa tagtacacct ctacttctaa aacattgtta gaaatcgatt
     2041 tgactgtcct
//
LOCUS       DAVKQL010000116         2025 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVKQL010000116 DAVKQL010000000
VERSION     DAVKQL010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.116"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            149..1495
                     /gene="gdhA"
                     /locus_tag="VW561_002073"
     CDS             149..1495
                     /gene="gdhA"
                     /locus_tag="VW561_002073"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEU9944269.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
BASE COUNT          645 a          372 c          387 g          621 t
ORIGIN      
        1 aaaacgttcg tttttttatc tattttcaaa aaaacccaaa aactgaattg aaagaatttt
       61 tagaaaattt ctgttttttc tttacagtga aaaaaaattc tgctataatg tcttatgtaa
      121 taaaatatga caataaaagg agaacgatat gacatctgct aaagaatata tccaaagcgt
      181 gtttgaaact gtgaaagctc gtaacgggca cgaggctgaa ttcctccaag ctgttgaaga
      241 atttttcaac actttagaac ctgtatttga aaaacaccct gagtatatcg aagaaaatat
      301 cttggcacgt attactgagc ctgagcgcgt ggtttctttc cgtgttcctt gggttgaccg
      361 tgatggaaaa attcaagtaa accgtggtta ccgtgttcaa ttcaactcag ctgttggacc
      421 atacaaaggt ggacttcgtt tccacccaac tgtaaaccaa gggatcttga aattcctcgg
      481 atttgaacaa atctttaaaa atgtcttgac tggacttcct atcggtggag gtaaaggtgg
      541 atcagacttc gatcctaaag gtaaaacaga tgctgaagtg atgcgcttct gccaaagctt
      601 catgactgaa ttgcaaaaac acatcggacc atcacttgac gtacctgctg gtgatatcgg
      661 tgttggtgga cgtgaaattg gttaccttta cggtcaatac aaacgtctta accaatttga
      721 tgctggtgtc ttgactggta aacctcttgg atttggtggt agcttgattc gtccagaagc
      781 aactggttac ggtttggttt actatactga agaaatgctc aaagctaacg gtaacagctt
      841 tgctggtaag aaagtcgtta tttcaggttc tggtaacgtt gctcaatatg ctcttcaaaa
      901 agcaactgaa ctcggtgcaa ctgttatttc tgtttctgac tcaaatggtt atgtcatcga
      961 tgaaaatggt atcgacttcg atcttttggt tgatgttaaa gaaaaacgtc gtgctcgttt
     1021 gactgagtat gcagctgaaa aagcaactgc aacctaccac gaaggtactg tatggactta
     1081 cgctggaaac tatgacattg ctcttccatg tgccactcaa aacgaaatca acggtgaagc
     1141 agctaaacgt ttggttgctc aaggcgttat ctgtgtatct gaaggtgcca acatgccgag
     1201 cgaccttgat gccatcaaag tttacaaaga aaatggtatc ttctacggac ctgcaaaagc
     1261 tgccaacgct ggtggtgtag ccgtttcagc tcttgaaatg agccaaaaca gtcttcgcct
     1321 ctcatggact cgtgaagaag ttgatggccg tctcaaagac atcatgacaa acatcttcaa
     1381 cacagctaaa acgacttcag aaacatacgg tcttgataaa gactaccttg caggagctaa
     1441 cattgctgcc tttgaaaatg tagcaaatgc catgattgca caaggtattg tttaagattt
     1501 atttactcaa tcctcaatca ctacgattgg ggatttttag cttgaattaa aaaattggaa
     1561 aatctaatta aataaaatga aatgaaataa aaactgaaca aattgattgc ggaattcaaa
     1621 ccaatttcta acatacttct agaaattaat ttgacttttc taatcgattt gttcatatct
     1681 tatttcaatt tactatactt acttaaccct ttttatactc aatgaaaatc aaagagcaaa
     1741 ctaggaagcg agccgcaggc tgtacttgag tacggcaagg caaagctgac gtggtttgaa
     1801 gagattttca aagagtatta aacaaaaaga aaaaccgcta ctcctaagaa tagcgattta
     1861 atcatgtttt taagctacta ctgtagttgc tctattattt aagtgttatg aataacttca
     1921 atatctttaa agctttttgt tagatttata atttcaacta tagccatctc tactccttag
     1981 tttacagcaa aaaattatgc ttaattctta ttgtttttgt aaaca
//
LOCUS       DAVKQL010000117         2010 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVKQL010000117 DAVKQL010000000
VERSION     DAVKQL010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.117"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(<1..1770)
                     /locus_tag="VW561_002074"
     CDS             complement(<1..1770)
                     /locus_tag="VW561_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec-dependent serine-rich glycoprotein
                     adhesin"
                     /protein_id="HEU9944270.1"
                     /translation="MFKVMRGGVDTTQVMTETVEDKVSHSITGLDILKGIVAAGTVIS
                     GTVATQTKVFTNESAVLEKTVEKTDALATNDTVVLGTISTSNSASSTSLSASESASTS
                     ASESASTSASTSASTSASESASTSASTSISASSTVVGSQTAAATEATAKKVEEDRKKP
                     ASDYAASVTNVNLQSYAKRRKRSVDSIEQLLASIKAAVFSGNTIVNGAPAINASLNIA
                     KSETKIYTGTGRDSFYNIPIYYKLTVTNDGSELTFTYTVTYVDPITETLGNLSRMTSG
                     YSIYNSGTSTQTMLTLGSGLGKPSGVKNSITDKNGRQVRHYNTSTMTKQGSGYTWANG
                     AQMNGWQAKQGYGLTSSWTVSITGTDTSFTFTPYAAKTDKIRTNYFNGGGKVVESSTT
                     SQSLSQSKSLSVSASQSASASASTSASASASTSASESASTSASASASTSASESASTSA
                     SASASTSASESASTSASASASTSASESASTSASASASTSASASASTSASASASTSASA
                     SASTSASASASTSASVSASTSASASASTSASASASTSASASASTSASASASTSASESA
                     STSASASASTSASASASTSASASA"
     gene            complement(1768..1881)
                     /locus_tag="VW561_002075"
                     /pseudo
     CDS             complement(1768..1881)
                     /locus_tag="VW561_002075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KxYKxGKxW signal peptide domain-containing
                     protein"
BASE COUNT          532 a          425 c          450 g          603 t
ORIGIN      
        1 tgctgaagcc gaagcactcg ttgacgctga agccgacgca ctggtgcttg ctgaagctga
       61 agcactcgtt gatgcagatt cagacgctga tgtgcttgct gaggctgaag cacttgttga
      121 cgctgaagct gaagcactgg tgcttgctga ggctgacgca ctcgttgatg ctgaagctga
      181 agcactggtg cttgctgaga ctgatgcgct ggtacttgct gaagctgagg cacttgtcga
      241 tgctgaagcc gaagcactcg ttgacgctga agccgacgca ctggttgacg ctgaagctga
      301 agcactcgtt gatgctgaag ctgaagcact ggttgatgca gattcagaag cacttgttga
      361 tgcggaagcc gacgcactgg ttgatgccga ttcagatgca ctggtgcttg ccgaagctga
      421 agcactggtt gacgctgatt cagacgctga ggtgcttgct gaagccgaag cactcgttga
      481 tgccgattca gaagctgagg tacttgctga agctgaagca cttgtacttg ctgaggctga
      541 ggcgctttga ctagcactta ctgagagtga cttagactgt gaaagtgact gactggtcgt
      601 gctagattca actacctttc ctccaccatt gaagtagtta gttctaattt tatctgtttt
      661 agcagcgtaa ggggtaaatg taaaggatgt atccgttcca gtaattgata cagtccaaga
      721 tgatgttaat ccatatcctt gcttagcctg ccaaccattc atttgggcac catttgccca
      781 agtataccca ctaccctgct tcgtcattgt agatgtatta tagtgtcgaa cctgtctacc
      841 atttttgtca gtaatggagt tctttacacc tgaaggttta ccaagaccac tgccaagggt
      901 taacattgtt tgtgttgaag tacctgaatt atagatagaa tatccagaag tcattcttga
      961 aagatttcca agagtttctg tgataggatc cacatacgta accgtatagg taaaggtcaa
     1021 ttctgaacca tcatttgtca ctgtcaattt atagtaaatt ggaatattat aaaatgaatc
     1081 tctacctgta cccgtgtaaa tttttgtctc acttttagca atgtttagac ttgcattaat
     1141 tgcaggggcg ccatttacaa tcgtattgcc agaaaaaaca gcagctttta tagaagccag
     1201 caattgctcg atggaatcca ctgaacgctt gcgtcgctta gcataagatt ggagattgac
     1261 atttgtaact gatgctgcat aatcactagc tggtttctta cgatcttctt cgaccttctt
     1321 agcagttgct tctgtagcgg cagctgtttg tgatcctacc acagtagatg atgcagagat
     1381 acttgtcgaa gccgatgtac ttgctgattc tgatgcactt gtactcgctg aggtcgaagc
     1441 gctggttgag gctgactcag atgcagatgt acttgccgac tctgaagctg acaaactagt
     1501 tgaactcgct gaattacttg tagatatcgt acctagaact actgtatcat ttgttgccaa
     1561 agcatccgtt ttctctacag ttttttcaag tactgccgac tcatttgtaa ataccttcgt
     1621 ttgagttgca acggttccac ttatgacagt tcccgcagca actattccct tgaggatatc
     1681 aagcccagta attgaatgac ttactttatc ttctaccgtt tcggtcatga cctgagtagt
     1741 atcaacacca ccacgcatga ccttaaacaa gccaaaaaga gaagtcgagg cccgtagcca
     1801 atgcttccct gatttgatta atttgaaccg agttactcgg tctgtttctc tatattcacc
     1861 cttctgacgt ctaaaaaata ttttgaaatt tcctttcgag tattattttc atgtatggat
     1921 ataaataaat taccatacta taattataga atagggaacc caaaatagca agaaacttga
     1981 ggtatttttg ctaaaaaaaa aagcatttta
//
LOCUS       DAVKQL010000118         1993 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVKQL010000118 DAVKQL010000000
VERSION     DAVKQL010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.118"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            445..1920
                     /locus_tag="VW561_002076"
     CDS             445..1920
                     /locus_tag="VW561_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU9944271.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTDKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSILLIGEQMNSEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEKVIELSEYARREINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDDSVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
BASE COUNT          595 a          370 c          448 g          580 t
ORIGIN      
        1 ttttttatca aaaaatactt tacaagttct taaaaacatg atatagtaat aaagcttaga
       61 aaatgagatg atgttttcta gcaaatataa acccgagtaa aaaatgccta cggacaggca
      121 gggttgaatg ccgaagcgtg gttgaaaagc cacattattg atagggttaa aagcctactt
      181 ttataagttg atgttaggac acttgttcta attcataaat ttttagtgtg gtgaaagcac
      241 acgtcatctt gtgaaacgat caataaagta cgtaatattt gctactagag agttaggaaa
      301 catcgggaac agacatactc aacagaaacc aaaataaaca cgtcagaaga ttgcagagca
      361 ggtgaaaacc tgctcttttt tcatgagtca acctttagtt ccttagtttt cataaggtcc
      421 taaaaatatt gaaaggagta tgttttgaaa gagttagatc aaaaccaagc cccaatttat
      481 gaggccttgg tgaagttacg caagaaaagg attgttccct ttgatgttcc aggtcacaag
      541 cgtggacggg gaaatccaga acttgtcgaa ctcttaggag aaaaatgtgt aggcattgat
      601 gtcaattcga tgaaaccctt ggataattta ggccatccta tttcgattat tcgtgatgca
      661 gaggagctgg ctgcagatgc ttttggagct agccatgcct ttctaatgat tggtggaaca
      721 acttcatcgg tgcagactat gattctggca acctgcaagg caggagataa gattattctg
      781 ccacgaaatg tccataaatc tgctatcaat gcgttggttc tatgtggtgc cattcccatc
      841 tatatcgaga tgagtgtaga tcctaagatt ggtatcgctt taggtcttga aaatgaccga
      901 gtagcacagg ccataaagga ccatccagat gctaaggcta tcctaatcaa caatcctact
      961 tactacggca tctgttcaga cctaaagggg ttgacagaaa tggctcatga agctggcatg
     1021 atggttttag tagatgaagc ccacggagcg catttgcatt tcactgataa acttccaatt
     1081 tctgctatgg atgcaggggc tgatatggca gcagtttcca tgcataagtc tggtgggagt
     1141 ttgacccaaa gctccatttt acttatcggg gagcagatga attctgaata cgttcgtcag
     1201 ataattaacc tgacccagtc tacatctgcc tcttacttgt tgatggctag tttggatatt
     1261 tcacgtcgca acttggccct tcgtggtaaa gagtcgtttg agaaagtcat tgagctatct
     1321 gagtatgccc gccgtgaaat caatgctatc ggtggctact atgcctactc aaaagagtta
     1381 atagacggtg tttcggtttg cgattttgac gtaactaagt tgtcagttta cactcagggt
     1441 attggcttaa caggtatcga ggtttatgac ctcttgcgag acgaatacga cattcagatc
     1501 gagtttggtg atatcggcaa tatcttggcc tatatttcca tcggcgaccg catccaagac
     1561 atcgagcgct tggttggtgc tctggctgat attaagagac tctattcaag agatggaaaa
     1621 gatttgatag caggagaata tattcagccc gagttagtgc tgtctccgca agaagccttc
     1681 tattcagaaa gaaaaagttt aactttggat gattctgttg gacaggtctg tggagaattt
     1741 gtcatgtgtt accctccagg tattcctatc ttggctcctg gagaacgcat tacacgagaa
     1801 attgtcgact atatccaatt cgccaaggaa cgtggttgct ccctccaagg gacggaagat
     1861 ccagaggtca atcatatcaa cgttattaag agaaagacaa actataagaa aagtcaatag
     1921 ttttatctaa actatttctt atttcaattt gatgatttgg cgatgatttt agagcacggc
     1981 aaaaagccct tga
//
LOCUS       DAVKQL010000119         1986 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVKQL010000119 DAVKQL010000000
VERSION     DAVKQL010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.119"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            <1..566
                     /locus_tag="VW561_002077"
     CDS             <1..566
                     /locus_tag="VW561_002077"
                     /inference="COORDINATES: protein motif:HMM:NF017515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944272.1"
                     /translation="PNILNDFNKGKISVQTFSDVLRFNLLKNNGGMWVDSTIYFTKKI
                     DLFQELEKCSFSTLNFSTSKDFMSYKGEVCTWSGFMIASHKQGRLVSIINELFERYYL
                     KYNTFSTYFFIDIVFMLCKVYKLDDNVLDQIILKDGNMFQLASVLDQPFNKSLLPYIE
                     KLPQKLFLHYEDNNLDNFYKYFKEIVS"
     gene            569..>1986
                     /locus_tag="VW561_002078"
     CDS             569..>1986
                     /locus_tag="VW561_002078"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944273.1"
                     /translation="MSNRLVNTRRNIVWGTLNKLIAIALPFLVRTTLIYQLGIQYVGI
                     SGLFASILQVLSLADLGFANAIVYSMYKPLAHGDNDTVGALLGFYKKIYRVVGVVILI
                     AGLLIVPFINYLVKGSYPADINIQLIYLIYLANTVVSYFLFAYKRSLLLASQRKDIFD
                     NIDSVARIILSGLQILLLFLFPDFYVFALVIPCMTILNNVFNEIVTKKNYNYIVENSN
                     LDKKVKQEIIEKTKGLFVYKVCGITRNSLDNIFISTFLGITTVGIYSNYYYIMISVKG
                     FLDVVSTGMSASVGHSVATESVEKNHRDLENLTFGFSWLATWFMTCLLCLFQPFMLLW
                     VGQANILPFSVVVALCFYFYVLAAGDMRSQYIDASGLWDKEKLRSIAETVGNVVLNYI
                     LVQFLGVLGIVLATALTILFIGIPWSTKIVFDNYFKNGYKKYLWNQAVYAIVTIIVAS
                     ITYLICTLVGGNNIVVLIIRGI"
BASE COUNT          636 a          260 c          340 g          750 t
ORIGIN      
        1 atccaaatat attgaatgat ttcaataaag gaaaaatttc tgttcaaact ttttcagatg
       61 tactccgatt taatctacta aaaaataacg gaggaatgtg ggttgactcc actatatatt
      121 ttactaagaa aatagattta ttccaagaat tggaaaaatg ttcattttca acattgaatt
      181 tttcaactag taaagatttc atgtcctata agggagaagt ttgtacatgg tctggattta
      241 tgattgcttc tcataaacag ggacgcctag ttagtatcat caatgaactt tttgaacgct
      301 actatttaaa gtataataca ttttctacat acttctttat agatattgtt ttcatgctct
      361 gtaaagttta caaactagat gacaatgtgt tagatcaaat cattttgaag gatggaaaca
      421 tgttccaatt agcttcagtt ttagatcagc cgttcaataa atctctattg ccatacatag
      481 aaaaactccc gcaaaaacta tttttgcatt acgaggataa taatttggat aatttttaca
      541 aatactttaa agaaatagtg agttaaccat gagtaataga cttgttaata ctagaagaaa
      601 tattgtttgg ggaacattaa ataagcttat tgcgattgct ttaccatttt tagttcgaac
      661 aactttgatt tatcaattag ggatacaata tgtcggtatt agtggacttt ttgcatctat
      721 tttacaagtt cttagtttag ctgatttagg tttcgctaat gcaattgtat atagtatgta
      781 taaacctctt gcccatgggg ataatgacac tgtcggtgca cttctaggat tttataaaaa
      841 aatatatcga gtcgtcgggg tggtaatatt aattgcgggc cttttaattg tgccctttat
      901 aaattactta gtaaagggtt cctatcctgc agatatcaat atacaactaa tttacttaat
      961 ttatctggca aatacagttg ttagttactt tctttttgct tataaacgct ctttattgtt
     1021 agctagtcaa cgaaaagata tatttgacaa cattgattca gttgctcgca taattttaag
     1081 tggattacaa attttattat tgtttctttt tcctgatttc tatgtttttg ctttagtaat
     1141 tccctgtatg acaatcctta ataatgtatt taacgaaatc gtgactaaga aaaattacaa
     1201 ttatatcgtt gaaaatagta acttagataa aaaagttaaa caagaaatta ttgaaaaaac
     1261 aaagggatta tttgtctaca aagtatgtgg gattacaaga aattccctcg ataacatctt
     1321 tatttctact tttttaggga ttacgacagt gggaatttat agtaattatt attacattat
     1381 gatatcagta aaaggttttt tggatgtggt ttctacagga atgagtgcta gtgttggaca
     1441 tagtgttgct actgaaagtg ttgaaaaaaa tcatcgtgac ttagaaaatc ttacatttgg
     1501 tttctcttgg ttagctacct ggtttatgac atgtttatta tgtctgttcc aaccatttat
     1561 gcttttgtgg gtaggacaag ctaatatttt accattttca gtcgttgttg ctctctgttt
     1621 ttacttttat gttcttgctg caggtgatat gcggagccaa tatattgacg cctcaggttt
     1681 atgggataaa gaaaaactac ggtcaattgc agaaacggta ggtaatgttg ttttgaatta
     1741 tattttggtt caatttttag gtgttttagg aatagtgcta gctactgcac taacaatttt
     1801 gtttattgga attccatgga gtacaaaaat tgtgtttgat aattatttta aaaatggtta
     1861 taaaaagtat ttgtggaatc aagcagtata tgctatagta actataattg ttgctagtat
     1921 tacttatcta atctgtacac tagttggtgg caataacatc gtggttttaa ttataagagg
     1981 aataat
//
LOCUS       DAVKQL010000120         1885 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVKQL010000120 DAVKQL010000000
VERSION     DAVKQL010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.120"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            <1..>1885
                     /locus_tag="VW561_002079"
     CDS             <1..>1885
                     /locus_tag="VW561_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HEU9944274.1"
                     /translation="ETDVQPESPDTVVSDKGEPKQVAPLPEYTGPQVSAIVEPEQVAP
                     LPEYTGVQAGSIVEPEKVEAPKEYTGKIEQPSAEDTKPENGASSTNGESERPKDKIKE
                     EKQVDKKLELRNVSNVELYTVENNKYRHITAVDGALDSSLKYFMKVKSENFKDIMLPV
                     TKIESTTKNNKEVYKIVAHAENLIQHENNVISNDYTYYLPKTQQSETGVYTSFKNLVD
                     AMNSDPNGTFHLGATMDAREVELPDDQESYVKNEFYGKLIGKNNGKYYAIYNLKKPLF
                     KTLNTATIQNLSIKEANVSSKEDAATISKEAKYNTLIDNVHSDGIIAGERGIGGLVSK
                     VDNSRISNSSFTGRITNTYDTTAGYEIGGLVGKLSGSLASIEKSIASIDIASNAKSGD
                     QIVGGIAGVVEKSATIKYSYVEGNVNNVRHFGKVGGVAGNLWDRDSQDVSKSGKLSYV
                     LSDVNVTNGNAIAGYNFNGIKTIETYSNKNNKVVNVVQEDDEVVTKDSDVQRGTVLDA
                     DKVKEKKVELVSKHSTKVEDFDFTSRYNTNYNEVTGYQQSREQVYKNIEKLLPFYNRE
                     TIVKYGNLVEDNSDLFTKKLLSVVPMKNNEVITDINKNKQEINKLLLHFEGNKSRVLN
                     IAYK"
BASE COUNT          753 a          250 c          368 g          514 t
ORIGIN      
        1 gagactgatg ttcaaccaga gtcgccagat actgtggtaa gtgataaagg tgaacctaaa
       61 caggtagcac cgcttccaga atatacagga ccacaagtca gcgctattgt tgaaccagag
      121 caggtagcac cactaccaga atacacaggg gtacaagccg gatcgatagt cgagccagag
      181 aaagtagaag caccgaaaga atatactggt aaaatcgaac agcctagtgc agaagatact
      241 aaacctgaaa atggggcaag ctctacaaat ggggaatcgg aacgtcctaa agataaaatt
      301 aaagaagaaa aacaagtcga caagaaacta gaacttagaa atgtttcaaa cgtggaatta
      361 tatacggtag aaaataataa atatcgacat attacggctg tagatggtgc tttagatagt
      421 tcattgaagt atttcatgaa ggtaaaatca gaaaatttta aagatataat gttgcctgtt
      481 actaaaattg aaagtacaac aaaaaacaat aaagaagtat acaaaattgt ggctcatgca
      541 gaaaatctaa ttcaacacga aaacaatgtt atttcgaatg actatacata ttatttacca
      601 aaaactcagc aaagtgaaac tggcgtctat acatcattta aaaatttagt tgatgcaatg
      661 aatagtgacc ctaatggaac attccattta ggtgcaacta tggatgctcg tgaagttgaa
      721 cttccggatg atcaagaaag ttatgtaaaa aatgaatttt acggaaaatt aataggtaaa
      781 aataatggta agtattatgc tatttataac ttgaaaaaac ctttatttaa aactctaaac
      841 accgctacta ttcagaatct ttcaataaaa gaagcgaatg tttcttctaa agaagatgct
      901 gctacaatat cgaaagaagc aaaatacaat actcttatag ataatgttca ttctgatggt
      961 attatagctg gtgaacgtgg aattggtgga ttggtttcaa aagtagataa ctcgagaatt
     1021 tctaatagta gttttacagg cagaataact aacacatatg atacaactgc agggtatgaa
     1081 attggtggtt tagtaggtaa actttctggt tcgcttgctt ctatagagaa atcaatagca
     1141 tcgattgaca tagcctctaa tgcaaaatca ggagatcaaa ttgttggtgg tatagccgga
     1201 gttgttgaga aaagtgcgac aattaagtat agctatgttg aaggaaatgt taacaatgtg
     1261 cgacactttg gtaaagttgg gggagttgca ggtaatcttt gggatagaga ttcacaagat
     1321 gtaagtaaat caggtaaact atcatatgtt ttaagtgatg tcaatgttac gaatggtaat
     1381 gctatagctg gctataattt caatggaata aaaacgattg aaacatatag caataaaaat
     1441 aataaggtag taaacgtagt acaagaagat gatgaggttg tgactaaaga ttcagatgta
     1501 caaagaggta cggtattaga tgctgataaa gtaaaagaga aaaaggtaga attggtttct
     1561 aaacacagca ctaaagtaga agattttgat tttacttcaa gatacaatac taattacaat
     1621 gaagtaacag gttaccaaca gtcgagagaa caagtatata aaaatattga aaaattactt
     1681 cctttctata acagagaaac aatagttaag tatggtaatt tagtagagga taacagtgac
     1741 ttatttacta aaaaacttct atcagttgtt cctatgaaga acaatgaagt aataactgat
     1801 attaataaaa ataaacaaga aataaacaaa ttgttattac attttgaagg taacaaatca
     1861 cgagtattaa atattgcata taaga
//
LOCUS       DAVKQL010000121         1852 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVKQL010000121 DAVKQL010000000
VERSION     DAVKQL010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.121"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            34..171
                     /locus_tag="VW561_002080"
     CDS             34..171
                     /locus_tag="VW561_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HEU9944275.1"
                     /translation="MAEEVLNLQLVSVQVDETDEVDGMRFSTFSTNRCGNWSAFSWEN
                     C"
     gene            467..1818
                     /locus_tag="VW561_002081"
     CDS             join(467..908,908..1818)
                     /locus_tag="VW561_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU9944276.1"
                     /translation="MKLSYEDKVQIYELRKQGQSFKQLSKRFGVDVSGLKYMVKLIDR
                     YGIEIVKKGKNRHYSSKLKQEMMDKALLEGCSQRSISLDYALPNQGMLSFWPAQYKKN
                     GYTIVEKTRGRPAKMGRKRKKTWEEMTELERLQEENERLRTEVAYPKKVKRVRGKGRS
                     PRARKAETVREMVSGGFRLDFLLETARLARSTYYYQLKQLDGVDKDKEIKTEIQGIDN
                     EHKGNYGYRRIHLELRNRGFVVNHKKVQRLMRILGLTARIRRKRKYSSYQGEIGKKAE
                     NLIQRQFEASRPMEKCYTDVTEFAIPNSTQKLYLSPVLDGFNSEIIAYHLSTSPNLEQ
                     VKSMLEQAFTEKYYENTILHSDQGWQYQHDSYHRFLESKEIQASMSRKGNSPDNGMME
                     SFFGILKSEMFYGYEKNFRSLENLEQAIVDYIDYYNNKRIKVKLKGLSSVQYRTKSFG
                     "
BASE COUNT          639 a          287 c          408 g          518 t
ORIGIN      
        1 taatcccaaa aatttaaata ggaggagcta gctatggcag aagaagtatt aaatcttcaa
       61 ttagtatctg ttcaagttga cgaaacagat gaagttgatg gaatgcgatt tagtacattc
      121 agtacaaacc gttgcggtaa ctggagtgct tttagttggg aaaattgtta attcatttaa
      181 attgtaaact ttagggattc tctgaagtta tggatgtcgt tccaatatgt gcacgttgga
      241 atgttagtgc ttatatgggg ggaacagaat cctctcttga ttgaagacaa gttagccatt
      301 aggctggttt gtctctttgt caactgtagt gggttgatat aatagtatta gtgagtggga
      361 taaaagtttc atttagttta ttcagtacaa atttaacggg tcaagattta ttgaactgca
      421 ccccaatagt tagacagaaa aaatctaact tttggggtgt tttattatga aattgagtta
      481 tgaagataaa gttcagatct atgaactaag aaagcaagga caaagcttca aacagctttc
      541 aaaaagattt ggtgtggatg tttctggtct aaagtacatg gtgaaattaa ttgaccgtta
      601 tggcatagaa atcgttaaaa aagggaagaa tcgtcactat tcttctaaat tgaaacaaga
      661 aatgatggat aaagccttac tggaaggttg ttcacaaaga agtattagtc ttgattatgc
      721 cctcccaaac caaggaatgc tttcattttg gccggcacaa tacaagaaaa acgggtatac
      781 tattgttgag aaaacaagag ggagaccagc taaaatggga cgtaaacgga agaagacttg
      841 ggaagaaatg acagaactag aacgactcca ggaggagaat gaacgcttac ggactgaggt
      901 ggcctaccta aaaaagttaa aagagttaga ggaaagggac gaagccctag agcgagaaag
      961 gcagagacag ttagagaaat ggtttcagga ggatttcgac tagattttct tcttgaaaca
     1021 gcacgtttag ctcgctcgac ttactattat cagttgaaac aactggatgg ggttgataaa
     1081 gataaagaga ttaaaaccga aattcagggc attgataatg aacataaagg caactatggc
     1141 tatcgtcgaa tccatttaga actaaggaat cgtggttttg tggtcaatca taagaaagtt
     1201 caacggttga tgagaattct tggtttaacg gctcgaattc gccgtaaacg gaagtattct
     1261 tcctaccaag gagagattgg caagaaagca gagaatctca ttcaacgcca gttcgaagca
     1321 tctaggccaa tggaaaagtg ctatacagat gtgacagagt ttgccattcc aaatagcaca
     1381 cagaaactct atttatcacc agttttagat ggttttaaca gtgaaatcat tgcttatcat
     1441 ctttctactt cgccaaactt agaacaagtg aagagtatgt tagaacaggc ctttacagag
     1501 aaatactatg agaatacgat tctccatagt gaccaaggct ggcaatacca acacgattct
     1561 tatcatcggt tcctagagag taaggaaatt caagcatcca tgtcacgcaa gggcaacagc
     1621 ccagacaacg gcatgatgga atctttcttt ggcattttaa aatccgaaat gttttatggc
     1681 tatgagaaga actttagatc tttagaaaac cttgaacaag ctattgtgga ctacattgat
     1741 tactacaaca acaaacgaat taaggtaaaa ctaaaaggac ttagttctgt gcagtacaga
     1801 actaaatcct ttggataaat tatttgtcta attttttggg gtcagtacag ta
//
LOCUS       DAVKQL010000122         1829 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVKQL010000122 DAVKQL010000000
VERSION     DAVKQL010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.122"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            68..1699
                     /locus_tag="VW561_002082"
     CDS             68..1699
                     /locus_tag="VW561_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEU9944277.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
BASE COUNT          525 a          384 c          364 g          556 t
ORIGIN      
        1 agatttactt ttatccttta aaatagaata ggagatattc cgcgattatt tttcggagga
       61 aaaagaaatg tccattaatt ggcaggaaat tttatttcac tttttaggtg gtctggggct
      121 attcttatat agtatcaaga ccatgggaga cggtttacaa caagctgctg gagatcgcct
      181 tcgtttttac attgacaaat atactagtaa tcctttcttt ggagttctgg ttggtattgg
      241 gatgactgct ctaattcagt ctagttctgg tgtaacagtt atcacagtcg gcctggtcag
      301 tgccggtctc ttaaccttac gtcaggctat cgggattgtc atgggtgcta atattgggac
      361 aactgtcaca tcctttctca tcggttttaa attaggtaac tatgccctac ctatgctctt
      421 tatcggtgcc gtctgtctct tttttacaaa aaatcggaca gtcaataata tcggacgcat
      481 cctctttggt gtcggtggta tcttttttgc cctcaatctc atgagcggcg caatggctcc
      541 actcaaggat ttacaggttt ttaaggacta tatgattgag ctaagtaaaa atcctgtttt
      601 gggtgtcttt gtcggtactg gcttgacctt gctaattcaa gcttcttcgg ctaccattgg
      661 gattttacaa aacctctacg ccggcaatct aattgaccta cagggagctt tgccggttct
      721 atttggtgac aatatcggga caaccattac agccatcatt gcctctttag gggctaatat
      781 tgcagctaaa cgggtagcag gagctcatgt tgccttcaac gttatcggaa cagtcgtctg
      841 cgttattttt ctagttcctt ttactgtcct gattcattgg tttgaagcta cgctaaatct
      901 agcaccggaa atgaccatcg cctttgctca cggaaccttt aatattacca acaccattgt
      961 ccaatttcca tttatcggag ctctggctta ctttgtaacc aagattattc ctggagagga
     1021 cgaggttgtc aaatacgaac ccttatatct tgatgaacat ttcatcaaac aggccccatc
     1081 tatcgctcta ggaaatgcta agaaagagct cttgcactta ggaaactacg ctgctaaagc
     1141 ctttgacctt tcctataagt acatcattga cttggatgaa aaagttgctg aaaaagggca
     1201 taaaaccgaa gaagcaatta acaccatcga tgagcaatta acacgttatc tcattgccct
     1261 ttcaagcgaa gctctcagcc aaaaagaaag tgaagtgctt accaatatcc ttgattcctc
     1321 ccgtgatttg gaacggattg gagaccacac ggaggctcta ctcaatctga ctgactatct
     1381 tcaacggaaa aatgttgaat tttctgatgc cgccttgaaa gaattagagg aagtttaccg
     1441 ccaaactagt gactttatca aagatgctct ggatagtgtg gaaaacaatg atattgaaaa
     1501 agcacgcagt cttgtagaac gtcatgaagc aatcaataag atagaacgtg ttctcagaaa
     1561 aacccacatc aaacgcctca acaaaggcga atgttcaaca caagctgggg tcaactttat
     1621 cgacatcatc tcacactaca ctcgtgtatc agaccacgct atgaaccttg ctgaaaaggt
     1681 ttttgcagaa caaatctaag aaccaagaag ctatccatca taattggatg gctttttact
     1741 ttttcctaag caagactagg ctgaatgaaa ctgaaagagt attctgcaga tatatagtat
     1801 attgaaacta gaatagtaca cctctattt
//
LOCUS       DAVKQL010000123         1819 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVKQL010000123 DAVKQL010000000
VERSION     DAVKQL010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.123"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            4..77
                     /locus_tag="VW561_002083"
     tRNA            4..77
                     /locus_tag="VW561_002083"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38..40,aa:Asn,seq:gtt)
     gene            185..1035
                     /locus_tag="VW561_002084"
                     /pseudo
     CDS             185..1035
                     /locus_tag="VW561_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(1070..1819)
                     /locus_tag="VW561_002085"
                     /pseudo
     CDS             complement(1070..1819)
                     /locus_tag="VW561_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          580 a          306 c          364 g          569 t
ORIGIN      
        1 taatccgcca tagctcagtt ggtagtagcg catgactgtt aatcatgatg tcgtaggttc
       61 gagtcctact ggcggagtag tttagttaag aggtttagac ctcttttttg ttatgtaata
      121 ataatctatt tcgtgttata gtaaaatgaa ccaaaaatag tacacaatgt ggtataatct
      181 ttttatggca tagtcaatag attttcgtaa aaaagttctc tcttattgtg agcgaacagg
      241 tagtataaca gaagcatcac acgttttcca aatctcacgt aataccattt atggctggtt
      301 aaagctaaaa gagaaaacag gagagctaaa ccaccaagta aaaggaacaa aaccaagaaa
      361 ggttgataga gatagactta aaaactatct tactgacaat ccagacgctt atttgactga
      421 aatagcttct gactttggct gtcatccaac taccatccac tatgcgctca aagctatggg
      481 ctacactcga aaaaaagaac cacacctact atgaacaaga cccagaaaaa gtagccttat
      541 ttcttaagaa ttttaatagt ttaaagcacc tagcacctgt ttagattgat gaaacaggat
      601 tcgatactta tttttatcga gaatatggtc gctcattaaa aagtcagtta ataagaggca
      661 aagtatctgg aagaagatat cagaggattt ctttggttgc aggtctaaca aatgaatggt
      721 gagttaatcg ctccaatgac ttacgaagag acgatgacga gcgacttttt tgaagcttgg
      781 tttcagaagt ttctcttacc aacattaacc acaccatcgg ttattattat ggataatgca
      841 agattccata gaatggggaa gttagaactt ttatgcgaag agtttgggca taaactttta
      901 cctcttcctc cctactcacc tgagtacaat cctattgaga aaacatgggc tcatatcaaa
      961 aagcacctca aaaaggtatt accaagttgc aatacctttt acgaggctct tttgtcttgt
     1021 tcttgtttca attgactata ttagaggcga gacatttttc ggttctttgt caactgtagt
     1081 gggttgaaga aatcgaagat ctagaaagga caaatttcgt cctttctttt ttgaagtttt
     1141 caaagttcct aaaaccaaag gcattgtgct tgataagttt gatgagatta ttggtggctt
     1201 ccagtttggc gttggaataa ggtaattgaa ggccgttgac gattttttct ttatctttga
     1261 ggaaggtttt aaacagagtc tgaaaaagag gtggaaaagc aagagctgat agagattata
     1321 gctgatagag attatagtgg tgtttcaagt cttcgaatag ctcaaaagtt tatctagaat
     1381 ttctttatta gtcaagtgca tacgaaaagt agggcgataa aatcgtttat cactcagttt
     1441 ctgactatct tgttgaatga gctccagtag cgcttgatag ccttgtattc atgggatttc
     1501 gtatgatggc ttgtgttctg ctctcaagaa cagttatgat attgagctta tcaaagtcct
     1561 gagcaataaa gctcatctcc atctcccgat tgaaatagtc actccctgga ctgtttcaac
     1621 gtcctaggac ataatctcag gaagacgcga aaaatcatgc tcaaagtgaa aatcattgat
     1681 cttgcgaatg acagttgaag ttgaaataga caactgataa tcaatgtcgg tcatagaagt
     1741 cttttcaatt agcttctgag caatcttttg gttgatgata caaggaattt gatgattctt
     1801 cttgacgata gaagtttca
//
LOCUS       DAVKQL010000124         1776 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVKQL010000124 DAVKQL010000000
VERSION     DAVKQL010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.124"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(34..630)
                     /locus_tag="VW561_002086"
     CDS             complement(34..630)
                     /locus_tag="VW561_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9944278.1"
                     /translation="MKIENESKSYDKCILKNISLEIDKRIYRVEGENGSGKSTLLQLL
                     AGLDTFDSGLKNCVSKDVLYLDTNQLGVVPFTIEENLQLLCDSFQIHLSFEDKEQINT
                     FFDNKIGDNYMTASTGTKFKLGLSLIFAKNWDYIFIDETLSTVDARSIDMIAKRLISM
                     KESSTIIYVSHNLLNQELISKSQRILIEEGRVYEISNK"
     gene            complement(627..1109)
                     /locus_tag="VW561_002087"
     CDS             complement(627..1109)
                     /locus_tag="VW561_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEU9944279.1"
                     /translation="MRKSGFLIYYVLFCIVCFLLGLLLSFILKNETFATIPPKLELQF
                     TLTVLQANSTNFLLYLFCFPISPLLQLFDLLSLGIQLGISGQMIGIVATIKGILPHAL
                     IEIPNFIWYQGMSQYLLYRLLKDRGIKNILIYIKSNLKWYIISYLLLILAALIEGIKL
                     "
BASE COUNT          697 a          284 c          205 g          590 t
ORIGIN      
        1 taaataatca gattcattaa tttgtataaa ctattatttg tttgaaattt cataaacccg
       61 tccttcctct attaaaatcc tttgagattt gctaatcaat tcttgattca ataaattatg
      121 agatacatat ataatagtag agctttcttt catggaaatt aagcgtttag caatcatatc
      181 aatacttctt gcatccactg ttgataaagt ctcatcaata aaaatataat cccaattttt
      241 agcaaaaatt aaggataagc ctaatttgaa ttttgttcca gtagatgctg tcatatagtt
      301 atctccgatt ttattatcaa aaaaagtatt aatttgttct ttatcctcaa atgatagatg
      361 gatttgaaaa gaatcacaca acaactgcaa attttcttct atagtaaatg gaacaacccc
      421 aagctgattg gtatcaaggt atagtacatc tttggagaca caattcttta aaccactatc
      481 aaaagtatct agcccagcta aaagttgtag aagggtgctc tttcctgaac cattctctcc
      541 ttcaactctg taaattctct tatcaatctc tagtgatata tttttcaata tacatttatc
      601 ataactttta gattcatttt caattttcat aatttaattc cctctatcaa cgctgctaaa
      661 attaataaaa gataagaaat gatataccat tttaaattac tttttatata aataagtata
      721 tttttaattc ccctatcctt caataatcgg tataaaagat actgactcat accttgatac
      781 caaataaaat tcggtatttc aataagagca tgaggtaata ttccttttat tgtagctact
      841 attccaatca tctgaccaga tatacccaat tgtattccta aagatagcaa atcaaaaagt
      901 tgcaataagg gcgaaatcgg aaaacagaat agatatagca aaaaatttgt agaatttgcc
      961 tgtaaaactg tcaatgtaaa ttgtaattcc agtttcggag gtatagtagc aaaagtctca
     1021 ttttttaata taaagcttaa aagtaaacct agtaaaaaac agactataca aaataacaca
     1081 tagtaaatca gaaaaccact ttttctcatc taatgccccc tactaaaatt acaaatatta
     1141 tcagaaaaca atataaaacc agaaaactcc atctcaataa actaactgta cctttaatta
     1201 aaaatagagc tttcttataa tagacatagg ctataaaata attgaatgga atcaataaca
     1261 aattcaacaa aatacttttt atatcattac tataacttgt caccggaatc aaacataaca
     1321 acaatgaaaa ttgtaagaaa gagattaaat gcgtcaacat cattagttgt gcattcttaa
     1381 aaaagaaatt cattttagaa tgattaagag cagataattg aagtaaagct agatctaccc
     1441 caattgagga tttatgattc cctattattg cctccattaa tgtaatatat attgaggtat
     1501 aaagaaataa tattggtatt tttgtgtcat caattgataa catcgtcatc atataccaaa
     1561 atagatagga aagaagaata ctaatcaaaa taaaagaaaa agttaaccct ttatttaacc
     1621 aacaaaaacg aagcaatata aatagactgc tacgaaatct tttttgactc attcgactgc
     1681 ttttaaaata gaaattactc gcatgattat ctaatatccc catgactaaa ataaaataat
     1741 tgagtagtat gacataaatt acaacttgtc cttgtt
//
LOCUS       DAVKQL010000125         1756 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVKQL010000125 DAVKQL010000000
VERSION     DAVKQL010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.125"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(<1..988)
                     /locus_tag="VW561_002088"
     CDS             complement(<1..988)
                     /locus_tag="VW561_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9944280.1"
                     /translation="MYTIIKSNIKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMN
                     LERFLKLSIYQMIVWCGIIFFDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHS
                     KSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLM
                     IMLLVPKIFASKMREVSLNLTNQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAG
                     ILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTG
                     VIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYE"
     gene            complement(1075..>1756)
                     /locus_tag="VW561_002089"
     CDS             complement(1075..>1756)
                     /locus_tag="VW561_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9944281.1"
                     /translation="RIKLILSNEDLGATNARNIGIKNSRGKYISFLDDDDEYMPDRIL
                     KLMACFKKSRMKNLALVYSYGIIIYPNGTREEEKTDFVGNPLFVQMVHNIAGTSFWLC
                     KKEVLELINGFEKIDSHQDGVVLLKLLVQGYQIDIVREFLVNYYAHSKENGITGVTQK
                     TINADEEYYNYCRKYFNLLSFNERILVTKKYYSLNIKRLLLIGDKCKALKVIKKAREE
                     KIFNEFLF"
BASE COUNT          611 a          315 c          202 g          628 t
ORIGIN      
        1 cttcatataa cggaaaatct ctattcacct catttggttc gatatcattc attttatttg
       61 actcaattgg attaattgaa tacaatttta aaaaaatagg cttcgtacca ataatagagg
      121 ataattgacc tcctaattca cctagcgctg taaaaataac acctgttagt gctcctattg
      181 cttcaatagt accaattttc actattcctt ttattgcaag atagcctgtt aaaaaaacga
      241 gagatatctg aaaaaaaata ttgagaaaga agctaatagc gcctgctaac gtttctacag
      301 ttgtctttct ttgtataacc atctttaata aaattcctgc ttctttaatt ttcttaggca
      361 atacatataa aagattcaag gacgctaaca catcaaatcc attcaatata gtctcactag
      421 attttaaaaa agcttcattt tggttagtta aatttagact aacttctcgc attttcgatg
      481 caaagatttt tggtacaagt agcataatca ttaatgaaaa caaggtggct acagtcaatg
      541 accaatgata gtgattaaga gtcacaactg caaatatagt accagaaatt ccttttatta
      601 ctaaaaaaag ttgtttaaaa gcctgatcat ttaaagtctg aacatcatta tttagccacg
      661 aaagatatgt tcctgatgat ttactatgaa attcttgata ggtagagtta gagatgtctg
      721 tggcaactct atttcgaatc tctagattaa actcttggat cacttcaacc tgataatttt
      781 tcactaccca gtcaaagaat attatcccac accagacaat catttggtag attgacaatt
      841 tcaaaaaccg ctctaaattc atcgcaatta attcattcaa caccagagca ttaatagttg
      901 ctgcataaat tagcaataat tgaccagcaa caataaatat cgttaataaa ctaaattttt
      961 ttatatttga ttttataata gtatacacaa tagtttctca ctttctaaat tttaattgaa
     1021 catagttgtc atatatacaa tagaaaaaac caaaattata taataacata tattttaaaa
     1081 aagaaattcg ttaaaaattt tttcttctct tgccttcttg attactttta aagccttgca
     1141 tttgtctcct attaatagta accgctttat gtttaaagaa taatatttct ttgtaaccaa
     1201 tattctctcg ttgaaactca ataaattaaa atatttccta cagtaattat aatattcttc
     1261 atctgcatta attgtttttt gtgtcactcc agtgataccg ttttctttac tgtgagcgta
     1321 gtaattcacc aagaattctc gcactatatc aatttggtat ccttgaacaa gtagttttaa
     1381 taaaacaaca ccgtcctgat gtgaatctat tttctcaaaa ccattaatta attctagcac
     1441 ctctttttta cacaaccaaa atgacgtacc tgctatattg tgaaccattt gaacaaacaa
     1501 gggatttcca acaaaatcgg tcttctcctc ttctcgtgta ccatttggat aaattattat
     1561 tccataacta taaactaaag ctaaattctt cattctactc tttttaaaac aagccatcaa
     1621 ctttaaaatt cgatctggca tatattcatc atcatcgtct aaaaatgata tatacttacc
     1681 tctagaattt ttgataccta tgtttctggc attagttgca cctaaatctt cattacttaa
     1741 aattaactta attcta
//
LOCUS       DAVKQL010000126         1516 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVKQL010000126 DAVKQL010000000
VERSION     DAVKQL010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.126"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            121..>1516
                     /gene="pcpA"
                     /locus_tag="VW561_002090"
     CDS             121..>1516
                     /gene="pcpA"
                     /locus_tag="VW561_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HEU9944282.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNGGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEI
                     HIKNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETD
                     VAKPKKNSNQGVVGW"
BASE COUNT          594 a          206 c          241 g          475 t
ORIGIN      
        1 aatttataaa atgtactact ctattctact agataatgat tgaataaaac tttttataac
       61 aacggctagc aaagttataa ttttatatct attagaaaat aaatatgtaa ggaaatttaa
      121 atgaaaaaaa ctacaatatt atcattaact acagctgcgg ttattttagc agcatatgtc
      181 cctaatgaac caatcctagc agatactcct agttcggaag taatcaaaga gactaaagtt
      241 ggaagtatta ttcaacaaaa taatatcaaa tataaggttc taactgtaga aggtaacata
      301 ggaactgttc aagtgggtaa tggagttact cctgtagagt ttgaagctgg tcaagatgga
      361 aaaccattca cgattcctac aaaaatcaca gtaggtgata aagtatttac cgttactgaa
      421 gtagctagtc aagcttttag ttattatcca gatgaaacag gtagaattgt ctactatcct
      481 agctctatta ctatcccatc aagcataaaa aaaatacaaa aaaaaggctt ccatggaagt
      541 aaagctaaaa ctattatttt tgacaaaggc agtcagctgg agaaaattga agatagagct
      601 tttgattttt ctgaattaga agagattgaa ttgcctgcat ctctagaata tattggaaca
      661 agtgcatttt cttttagtca aaaattgaaa aagctaacct tttcctcaag ttcaaaatta
      721 gaattaatat cacatgaggc ttttgctaat ttatcaaatt tagagaaact aacattacca
      781 aaatcggtta aaacattagg aagtaatcta tttagactca ctactagctt aaaacatgtt
      841 gatgttgaag aaggaaatga atcgtttgcc tcagttgatg gtgttttgtt ttcaaaagat
      901 aaaactcaat taatttatta tccaagtcaa aaaaatgacg aaagttataa aacgcctaag
      961 gagacaaaag aacttgcatc atattcgttt aataaaaatt cttacttgaa aaaactcgaa
     1021 ttgaatggag gtttagaaaa aatcggtact tttgcatttg cggatgcgat taaacttgaa
     1081 gaaattagct taccaaatag tttagaaact attgaacgtt tagcctttta cggtaattta
     1141 gaattaaaag aacttatatt accagataat gttaaaaatt ttggtaaaca cgttatgaac
     1201 ggtttaccaa aattaaaaag tttaacaatt ggtaataata tcaactcatt gccgtccttc
     1261 ttcctaagtg gcgtcttaga ttcattaaag gaaattcata ttaagaataa aagtacagag
     1321 ttttctgtga aaaaagatac atttgcaatt cctgaaactg ttaagttcta tgtaacatca
     1381 gaacatataa aagatgttct taaatcaaat ttatctacta gtaatgatat cattgttgaa
     1441 aaagtagata atataaaaca agaaactgat gtagctaaac ctaaaaagaa ttctaatcag
     1501 ggagtagttg gttggg
//
LOCUS       DAVKQL010000127         1468 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVKQL010000127 DAVKQL010000000
VERSION     DAVKQL010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.127"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            149..331
                     /locus_tag="VW561_002091"
     CDS             149..331
                     /locus_tag="VW561_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HEU9944283.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGEKIILGIDE
                     KNIDTYQRVNRLPAKL"
     gene            276..1125
                     /locus_tag="VW561_002092"
                     /pseudo
     CDS             276..1125
                     /locus_tag="VW561_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="programmed frameshift; internal stop; incomplete;
                     partial in the middle of a contig; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(1189..1455)
                     /locus_tag="VW561_002093"
     CDS             complement(1189..1455)
                     /locus_tag="VW561_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944284.1"
                     /translation="MDNVEWSHADSYFLSFVSDDVEERYTENVYLDSLSVKQKFKFIF
                     DFGDEWRFECQVLREIETEDEEAYLVRSVGTSPEQYPDYDGFDY"
BASE COUNT          530 a          281 c          262 g          395 t
ORIGIN      
        1 atttcttttt taacccgtgt taaaaaagaa attaaagtgg gttaaaaaag aaattaactg
       61 agtaaaaact ctcgaaattc aattcgctat attatcactc aacttgaaca aataccagaa
      121 ccacaaaaaa cagatgtaaa ggaaaaatat ggacttaaaa tttgaagggg ttgatttaga
      181 atacaaaaaa gctaaaaaca atcttccaga gtctttctgg gaaacctact cagcatttgc
      241 aaatacaaat ggcgagaaaa taattctcgg gatagatgaa aaaaacattg acacctacca
      301 aagagtcaat agactccctg cgaaattatg aagcaagtaa acaactaacc gatgcacgat
      361 ttaaatgtct tgttggtgtt caacgcacga cttttgaaga gatgttagct gtattaaaaa
      421 cagcttatca acttaaacac gcaaaaggtg gacgaaaacc taaattaagc ctagaagacc
      481 ttcttatggc cactcttcac tatgtgcgag aatatcgaac ttatgaagaa attgcggctg
      541 attttggtat ccacgaaagc aacttactcc gtcggagcca atgggttgaa gtaactcttg
      601 ttcaaagtgg tgttacgatt tcaagaactc ctctcagctc tgaggacacg gtaatgatta
      661 ttgatgcgac ggaagtataa atcaatcgcc ctaaaaaaag aattagcgaa tgattctggt
      721 aaaaagaaat gccacgctat gaaggctcaa gcgattgtca caagtcaagg gagaattgtt
      781 tctttggata tcactgtgaa ctattgtcat gatatgaagt tgttcaaaat gagtcgcaga
      841 aatatcggac aagctggtaa aatcttgact gacagtgatt atcaagggcc catgaagata
      901 tatcctcaag cacaaactcc acgtaaagcc agcaaactca aaccgctaac agctgaagat
      961 aaagcctgta accatgcact atctaaggat agaagcaagg ttgagaacat ccttgccaaa
     1021 gtaaaaacgt ttaaaatgtt ttcaacaacc tatcgaaatc atcgtaaacg cttcggatta
     1081 cgaatgaatt tgattgctgg tattatcaat catgaactag aattttggtt tcgcaggaag
     1141 tctattgaaa tactaagcct acacagactg atttcaattt taccattctt aatagtcaaa
     1201 accatcataa tctggatatt gttctggcga cgttccaacc gaacgtacga gataagcttc
     1261 ttcgtcctct gtctcgattt ctctcagcac ttggcattca aaacgccatt catcaccgaa
     1321 gtcgaaaata aacttaaatt tttgtttgac acttaggcta tccagataga cattttctgt
     1381 gtaacgttct tcaacatcgt cactaacaaa gctaagaaag taagaatctg catgactcca
     1441 ctcaacatta tccatgaaaa atgcatga
//
LOCUS       DAVKQL010000128         1388 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVKQL010000128 DAVKQL010000000
VERSION     DAVKQL010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.128"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            <1..569
                     /locus_tag="VW561_002094"
     CDS             <1..569
                     /locus_tag="VW561_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9944285.1"
                     /translation="QRNEKYLILGESGSGKSTLLKLLNGFLRDYSGELRFCGDDIKKT
                     SYLNMVSNVLYVDQKAYLFEGTIRDNILLEENYTDEEILQSLEQVGLSVKDFPNNILD
                     YYVGDDGRLLSGGQKQKITLARGLIRNKKIVLIDEGTSAIDRRTSLAIERKILDREDL
                     TVIIVTHAPHPELKQYFTKIYQFPKDFI"
     gene            589..>1388
                     /locus_tag="VW561_002095"
     CDS             589..>1388
                     /locus_tag="VW561_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944286.1"
                     /translation="MRFYCEAYEVITEEKKVYFNDIELEVKYSSVEELFIICEKLLDK
                     KKVSFFYVDEKPLRYLLFDYIFLLVLAKKNIPILDGVSNKQVDPTLLHHFSLEIEKNF
                     IDFCYKNMDLILKTQSISLCHREELIIVDVSDDSKFGVFYKRFRTLVDKSNGKYVCVY
                     NFSRVSDILQNWKNYCNRKFSVTFTESQFELFKLLYNQKNFKTISLLFGKKIVAGGII
                     YYSDLTNIEYFCIFWWDSMFGKDSIGKYVYVEEISRCHFLDRNYSFCYG"
BASE COUNT          490 a          140 c          263 g          495 t
ORIGIN      
        1 ttcaacgtaa tgaaaagtat ttaattttag gtgaaagtgg aagcggaaaa tctacattat
       61 taaaattatt gaatggcttt ttgagagatt atagtggaga attgcgattc tgcggggatg
      121 atataaaaaa aacctcctat ttaaatatgg tttcgaatgt tctatatgta gatcaaaaag
      181 cttatttgtt tgaaggtacg attagagata atattttatt ggaagaaaat tatactgatg
      241 aagaaatact acagtcttta gagcaagttg gtttgagtgt aaaagatttt cctaataaca
      301 ttttagatta ttatgttggt gatgatggga gattactgtc aggagggcag aaacaaaaaa
      361 ttactttagc tagagggcta attagaaata agaaaatagt attaattgac gagggaactt
      421 ctgctatcga taggagaact tcgttagcga ttgaacgtaa gatattagat agagaggatt
      481 tgactgtcat tattgttacc catgctccgc atccggaact taaacaatat tttactaaga
      541 tatatcaatt tccaaaggat tttatttaag aatatagggg agggaaacat gagattctat
      601 tgtgaagctt atgaggtaat tactgaagag aaaaaagtat actttaatga tattgagtta
      661 gaagttaagt attcctctgt cgaagaattg tttataatat gtgagaaatt attagataag
      721 aaaaaagtat cattttttta tgtagatgag aaacccttaa ggtatctgtt atttgactat
      781 atatttctac tagttctagc taaaaaaaat atcccaatct tagatggagt ttccaataag
      841 caagttgatc caacgttgct gcatcacttt tctttggaaa ttgaaaagaa ttttatcgat
      901 ttttgctata aaaatatgga tctcatccta aaaactcaaa gtatatcttt gtgtcataga
      961 gaagagctta ttattgtaga tgtttcagat gactctaaat ttggtgtttt ttacaagcgt
     1021 tttagaacac tggttgataa aagtaacgga aaatatgttt gtgtttataa tttcagtcgt
     1081 gtttcagata tactgcaaaa ctggaaaaat tattgcaatc gaaaattttc ggttacgttt
     1141 actgaaagcc aatttgaatt atttaagctt ttatataatc aaaaaaattt taaaacaatt
     1201 tcgttgctat ttggaaaaaa aattgttgct ggaggaatta tctattatag cgatcttact
     1261 aatatagaat acttttgtat tttttggtgg gatagtatgt ttgggaaaga ttctattgga
     1321 aaatacgtat atgtagaaga gattagtaga tgtcattttt tagatagaaa ttattctttt
     1381 tgttatgg
//
LOCUS       DAVKQL010000129         1300 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVKQL010000129 DAVKQL010000000
VERSION     DAVKQL010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.129"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(138..449)
                     /locus_tag="VW561_002096"
     CDS             complement(138..449)
                     /locus_tag="VW561_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944287.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCLFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(464..1192)
                     /locus_tag="VW561_002097"
                     /pseudo
     CDS             complement(464..1192)
                     /locus_tag="VW561_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
BASE COUNT          392 a          269 c          223 g          416 t
ORIGIN      
        1 aaaaaagtct ttaaaatctt aaaaacgcat agtatcaggt attgaacaat cttgatatta
       61 tgcgttttat tgtgggaaga tttacttcat tttctcttgg aattgagttt tctcctactt
      121 caaaactaga tgatttgcta tttaaaatag cgttccttat gattttggca acctggatta
      181 aagcgagaaa aacaaaagag gcaacttaag ctttctttat attgcttata tgttagtagt
      241 tttagacaaa caccacataa aataggctta tcctgtatca aagataaggg atacggctca
      301 aaagggtgat tttctaatga atcatgacac cgataacaag cataatactt tttacaatca
      361 tagcattgta aagcaataat atccagcttg ctatggtaat gaatgcatct tgtttgctta
      421 tccactaaga aaccctgtgc ctctatcatt tccttttcct ttcttaagct cgaactgtga
      481 cgctggttcc atcttcctta taaagattga tgagtccttc ttttaatgca cgaaccatgt
      541 ctcctgattg aacgggttga ggcaccttaa tagtcaatag ttccattgga tttggagcgc
      601 ggtcgatttt attgcctttg gcatcatgca aatcttcaat ataggtttca aaatgacgga
      661 aacctggacc ataaaactca acttggtccc cttcgttaat gacatttcgt tgacgaatag
      721 ttgctgtttg tgccgcatca tcataagaaa ccacttcagc gacaaacttg tactcaggaa
      781 ttttacgacg agcaccaaac aactgctcat tttcagatgg tgtaccatag taaaatcctg
      841 tagccagttc acgttgggca accttccaca tctcgtccac caagtcttgt ttgatagctt
      901 caaacttttc aggactttca agataggcat ccacagccgc cttgtagcag ttggttactg
      961 ttaatacata gtgaatagac tccatacgtc cttcgatttt tagactgtcc acaccatttt
     1021 caatcatatc tggaatgtgg tcaatcatag acatgtcaac ggctgacatt gaaaattctt
     1081 ctggaatctc accctgcaaa ctcttacgtt ctttcccaaa tggcatatcg taaaggtcgt
     1141 acttccaacg gcatgactga gaacatccac cacggttggc atcacgcata ctctagacgg
     1201 attgttttga caattctaat cttttagact ttgcataata actgttaaac agttgaaaat
     1261 tgacccagtt ttaacaagta gtcaaaatac cagataaagg
//
LOCUS       DAVKQL010000130         1275 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVKQL010000130 DAVKQL010000000
VERSION     DAVKQL010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.130"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(<1..1031)
                     /locus_tag="VW561_002098"
     CDS             complement(<1..1031)
                     /locus_tag="VW561_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU9944288.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESSEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAAQTAT"
BASE COUNT          378 a          303 c          227 g          367 t
ORIGIN      
        1 gttgctgttt gtgccgcatc atcataagaa accacttcag cgacaaactt gtactcaggg
       61 attttacggc gagcaccaaa caactgctca ttttcagatg gtataccgta gtagaaacct
      121 gttgccaatt cacgttgggc aaccttccac atctcgtcca ctaagtcttg tttgatagct
      181 tcaaactttt cagaactttc aagataggca tccacagccg ccttgtagca gttggttact
      241 gttgatacgt agtgaataga cttcatacgt ccttcgattt ttagactgtc cacaccattt
      301 tcaatcatat ctggaatgtg gtcaatcata gacatgtcaa cggctgacat tgaaaattct
      361 tctggaatct caccctgcaa actcttacgt tctttcccaa atggcatatc gtaaaggtcg
      421 tatttccaac ggcatgactg agaacatcca ccacggttgg catcacgcat actcatgtgg
      481 tttgaaagag tacaacgtcc agagtatgaa atacacatag ctccatggac aaaggcttca
      541 atttcaacat ctgtacgttt gcggatctca gctaattctt ccattgaaac ctcacgcgct
      601 aaaacgacac gagtcaagcc tagctctttc cagaactcaa gggtttcata gttagtggca
      661 ctggcttggg tagaaaggtg gatttcaagg cctggtgctt cagtcgctgc aatcataatc
      721 aaggctgggt cagatacgat aactgctgca atcccgatat cacgcagttt acggaaccac
      781 tcaccagcac cagcttcatt tccttcgtgc ataaccatat tagccgctac atagaccttg
      841 gcaccatact tggccgcaaa ctgcacgcct tcttccatct gttcgaaagt aaagtttccc
      901 gcacggctac gaagaccata ggcctgacca ccgataaaga cagcatctgc tccatactga
      961 acagctacct ttagcttctc taaagtccct gcaggtgata aaacctcagg acgttttaat
     1021 gtttttgtca taatttctcc tatttgcaat ataaaagttt gacatttttc cttctcattt
     1081 tatagtaaat aaaggataaa atcaagccta gacggattgt tttgacaatt ctaatctttt
     1141 agactttgta taataactat taaacagtcg aaaattaccc cagttttaac aagtagtcaa
     1201 aaagtctaaa aaagtaaaaa ccagtatccc aagatactga ctcgtaaaaa tatataaatt
     1261 agtccttaga tgact
//
LOCUS       DAVKQL010000131         1187 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVKQL010000131 DAVKQL010000000
VERSION     DAVKQL010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.131"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            4..480
                     /locus_tag="VW561_002099"
     CDS             4..480
                     /locus_tag="VW561_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9944289.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVASLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPGK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAEATA"
     gene            518..820
                     /locus_tag="VW561_002100"
     CDS             518..820
                     /locus_tag="VW561_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9944290.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFCIPAMSVPVFDNIILPAKQNMV"
     gene            828..1187
                     /locus_tag="VW561_002101"
                     /pseudo
     CDS             828..1187
                     /locus_tag="VW561_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT          379 a          219 c          238 g          351 t
ORIGIN      
        1 cttatggtat tagattattt ctttgacaaa aaccttgtgt tttgcttaga agcggataat
       61 caagaacaac tctttgatca ggtagcaagc ttgttggaag aacgagaaat tgtcactcct
      121 acttatcgtg aagccttgat cacgcgtgaa aagtcatttc caactggttt agatatggaa
      181 tttctaggaa aggacttgcc aaatgtagcg attcctcaca cagatattgt tcacaatctg
      241 gctgaaaaag ttgtggtcgt tcgattagag aaaccggtga ctttccataa tatgattgcg
      301 ccaggtaagg aagtagaagt atccttgctc ttctttatca tcaataattc aagttcaagt
      361 caaacaaata ttctggctca gttgatggac tttttcacag gaaatggtca tcttgaagac
      421 ctatcaaaaa tttctgaacc agaaaaactt tatgcttaca ttgctgaagc aaccgcttaa
      481 tcttgtctat taaaaaataa aatcggagga aatccaaatg attaaaattc ttgctgcctg
      541 cggtgcaggt gttaactcaa gtcaccaaat taaaagtgct ctagaagaag aactttcaaa
      601 ccgtggttat gatgttcact gtgatgcagt catggtgaaa gatgtaaacg aagaccttat
      661 gaaaggttac gatatcttta caccaatcgc tgcaacagac cttggttttg aaccaggtat
      721 cccagttatc gaagctggtc caatcttatt ctgtatccca gcaatgagcg ttccagtatt
      781 tgacaatatt atacttcctg cgaaacaaaa tatggtatag tagttctatg aatgatgaag
      841 caagtaaaca actaactgat gcacgattta agcgtcttgt tggtgttcag cgtaccactt
      901 ttgaagagat gttagctgta ttaaaaacag cttatcaact taaacacgca aaaggtggac
      961 gaaaacctaa attaagccta gaagaccttc ttatggccac tcttcatatg tgcgagaata
     1021 tcgaacttat gaacaaattg cggctgattt tggtattcac gaaagcaact ttatccgtcg
     1081 gagccaatgg gttgaagtaa ctcttgttca aagtggtgtt acggtttcaa gaactcctct
     1141 cagctctgag gacacggtaa tgattgatgc gacggaagta caaatca
//
LOCUS       DAVKQL010000132         1182 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVKQL010000132 DAVKQL010000000
VERSION     DAVKQL010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.132"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            231..>1182
                     /locus_tag="VW561_002102"
     CDS             231..>1182
                     /locus_tag="VW561_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9944291.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     HNEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQ
                     KFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGK
                     SIEERPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAESVN
                     QALTLLLGEHRILSITADNGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRR
                     WLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSEFLLGG"
BASE COUNT          411 a          206 c          248 g          317 t
ORIGIN      
        1 taccttagtc aagaattaga tgtttcacta tgtttgagta agttgatgat ttcatttgat
       61 aacaggtttg aaactgttag gctaggtggc caaggctaat catagccttg gtttagctga
      121 aaaaacaggt tcaagggttc ctgttgtcaa atgaaatgtg atttaaggta taagaaaaca
      181 cctctgtgct atacttgttg ttcaccacaa acacaaggaa aggcacagag atgcaagaac
      241 attatacccc aaaagggaaa catttgacaa tagataaccg tcgcttgatt gagcggtgga
      301 agaatgaaaa taagtccaat cgtgaaattg caggcttgtt aggaaaggcg cctcaaacga
      361 ttcataatga agtcaaaaga ggtacaactt tacaacaagt gagaaaaggg ctatacaaaa
      421 aggtctattc tgccgattac gcacaaactg tttaccaatt caatcgaaaa cggtcggtga
      481 aaaagttaat tttaacaaag gaaatcagag agaagatctt acactatcat aagcaaaaat
      541 tttcgcctga aatgatggtt aacaagaagc aagtgaaagt tggtatttca accatctact
      601 actggtttca taatggtcat ttaggattga cgaaggccga catgctttat cccagaaaaa
      661 ggaaaggtgt caagaagcaa gctagtccga actttaagcc ggcaggtaaa tctatcgaag
      721 aacgtcctga cgttattaat cttcgcttgg aaaatggtca ttatgaaatt gataccgtcc
      781 tactgactaa gataaaaaat tattgcctgt tagtcttaac cgaccggcgg agcagacacc
      841 aaattataag gttaattcca aataaaactg ctgaatctgt caatcaggcg cttacgttac
      901 tattagggga gcatcgtatt ctgtccatta ctgcagataa tggttcggag ttcaaacgat
      961 tgtctgaggt atttcctgag gaacatatct actacgcaca tgcttactct tcatgggaga
     1021 gaggttcaaa tgaaaatcat aatcgattaa ttcggagatg gttacctaaa ggaaccaaga
     1081 aaacgactcc gaaagaagta gcttttatcg aaaattggat taacaactac cctaaaaaat
     1141 gcttggacta caagtcgcca agtgaatttc ttttgggtgg ct
//
LOCUS       DAVKQL010000133         1129 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVKQL010000133 DAVKQL010000000
VERSION     DAVKQL010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.133"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(<1..965)
                     /gene="pspC"
                     /locus_tag="VW561_002103"
     CDS             complement(<1..965)
                     /gene="pspC"
                     /locus_tag="VW561_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     LPXTG-anchored form"
                     /protein_id="HEU9944292.1"
                     /translation="MFKSNHERRMRYSIRKFSVGVASVAVASLFMGSVVHATEKEGST
                     QAATSFNRGNGSQAEQRGELDLERDKAMKAVSEYVGKMVRDAYVKSDRKRHKNTVALV
                     NQLGNIKNRYLNEIVHSTSKSQLQELMMKSQSEVDEAVSKFEKDSFSSSSSGSSTKPE
                     TPQPENPEHQKPTTPSPDTKPSPQPEGKKPSVPDINQEKEKAKLAVVTYMSKILDDIQ
                     KHHLQKEKHRQIVALIKELDELKKQALSEIDNVNTKVEIENTVHKIFADMDAVVTKFK
                     KGLTQDTPKEPGNKKPSAPKPGMQPSPQPEVKPQPEKPKPEVKPQP"
BASE COUNT          260 a          219 c          208 g          442 t
ORIGIN      
        1 ggttgcggtt taacctctgg ttttggtttt tccggctgcg gtttaacctc tggttgagga
       61 cttggttgca tacctggttt tggagcagat ggttttttgt tacctggttc ttttggtgtg
      121 tcctgagtta agcctttttt gaatttagtc acaactgcat ccatgtctgc aaatatcttg
      181 tggactgtat tttcaatttc tactttggta tttacattat caatttcaga aagagcttgc
      241 tttttaagct catcaagctc cttaataaga gcaacaatct gacgatgttt ttctttctgc
      301 agatgatgtt tttgtatatc atctaaaatc ttgctcatgt aggttactac agcaagctta
      361 gctttttctt tttcctgatt aatgtctggt acgcttggtt tcttgccttc tggttgaggg
      421 cttggtttgg tatccggaga tggagttgtt ggtttttgat gctctggatt ttccggctgc
      481 ggagtttctg gtttagtgga ggatcctgaa cttgacgaag aaaatgagtc cttttcaaat
      541 ttagacacag cttcatctac ttctgattga ctcttcatca tcagttcctg tagttggctt
      601 tttgaggttg aatgaactat ttcattcaaa tacctgttct taatgtttcc caactggtta
      661 actagagcta cagtattttt atgtcgtttt ctatctgatt ttacataggc atctctcacc
      721 atttttccta catattcact gaccgctttc attgccttat ctcgttctaa atcgagttct
      781 ccacgttgtt ctgcctgact tccatttccc ctattaaaag aagtggctgc ttgggtactt
      841 ccctctttct ctgtcgcatg tacaacactt cccataaaaa gactggcaac agctacgcta
      901 gctactccta cactaaattt acgaatggaa taacgcattc ttcgctcatg atttgattta
      961 aacatgtcta tattctccat aattttaatt aactacagta tatcctgcta atatgaattg
     1021 tatatgaaat ttgctacatt atttggctct ataatatttg tagtgggtaa atcccctatg
     1081 gatattatgg agcctatttt gtgtagaaaa aaagtcccat atgacctat
//
LOCUS       DAVKQL010000134         1090 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVKQL010000134 DAVKQL010000000
VERSION     DAVKQL010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.134"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(110..346)
                     /locus_tag="VW561_002104"
     CDS             complement(110..346)
                     /locus_tag="VW561_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9944293.1"
                     /translation="MDVILSAIIFGISHLILSHRDPISLLYYSLIGFFFALVYRFTDN
                     LRLTILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(359..832)
                     /locus_tag="VW561_002105"
     CDS             complement(359..832)
                     /locus_tag="VW561_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944294.1"
                     /translation="MRNTKKLRQFGIFLLIILLSTYLPQTIGLYVTIILGLGADVYSL
                     ILTMVLVGSFLLLIWRLKKKKMLFIFEKKSWNWSFVFSLFATYVVYQILGNFWARYAH
                     LINHRNIHDEYFTVVLSNGQPTFLSTILSFVLPVIIGPVFEETLDRGYFMNTFFP"
     gene            complement(850..1035)
                     /gene="briC"
                     /locus_tag="VW561_002106"
     CDS             complement(850..1035)
                     /gene="briC"
                     /locus_tag="VW561_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEU9944295.1"
                     /translation="MTGTNTFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRAG"
BASE COUNT          421 a          195 c          168 g          306 t
ORIGIN      
        1 taggctccat aatatctata ggggatttac ccactacaaa tattatagag ccaacaataa
       61 aaagaaaaag tgtttgatag atatcaaaca cttttttctt tgcctcccac tatctaaaaa
      121 aatgataata gatataattg taaacaaaaa tccagatagg ttttgcatga ttgagaaagt
      181 taaaaaaact atggcagaga atcgttaatc tcagattgtc ggtaaaacga taaacaaggg
      241 caaaaaagaa accaatcaga ctataatata ataaactaat tggatctctg tgagatagta
      301 tcaaatggct aatcccaaag atgatagcag ataggataac atccaaataa tacttggact
      361 agggaaagaa ggtattcata aaataccctc tatcaagagt ctcctcaaaa acaggaccga
      421 tgattacagg caggacaaaa gataagatag tcgataaaaa ggttggttgt ccatttgaaa
      481 gaaccacggt aaaatactca tcatgaatat tcctatgatt aatcaaatga gcatagcgtg
      541 cccaaaaatt accgagaatc tgataaacca cataagttgc aaataaggag aagacaaatg
      601 accagttcca gctctttttt tcaaagataa agagcatctt tttctttttt aacctccaaa
      661 ttaatagaag gaaacttccc actaatacca ttgttaaaat aagagaatag acatcagctc
      721 ctaaccctaa aatgatcgtc acatacaatc caattgtttg tggtaaatag gtagatagta
      781 aaataataag caaaaatatt ccaaattgtc ttagtttctt tgtgtttctc atcgtacttt
      841 tttgaaagat taccctgctc ggaagccgta cttccaagca tctatataag aattaagtgc
      901 cccttgcctc atatagggag caaattctct ataatataac catctactat atccatcttc
      961 ccaaacagca agaccacctg aagtttgctc caagtcctca gttgaaagaa ctgtaaatgt
     1021 atttgtacct gtcattgcaa gtaccttctt aaaatatagt gatttgaaat aagatgtgaa
     1081 caactctatc
//
LOCUS       DAVKQL010000135          989 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVKQL010000135 DAVKQL010000000
VERSION     DAVKQL010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.135"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(<1..>989)
                     /locus_tag="VW561_002107"
     CDS             complement(<1..>989)
                     /locus_tag="VW561_002107"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944296.1"
                     /translation="ALVDADSEAEVLAEAEALVLAEADALVLAEADALVDADSEALVD
                     AETEALVDAEAEALVLAEADALVDAEAEALVLAETDALVLAEAEALVDAEAEALVDAE
                     AEALVDAEADALVLAEAEALVDADSDADVLAEAEALVDAEAEALVLAEAEALVDAEAE
                     ALVLAETDALVLAEAEALVLADSDALVLAEADALVDAEADALVDAEADALVLADSDAL
                     VLADSDALVLAEAEALVDAEADALVDAEADALVDAEAEALVDAEADALVLAEADALVD
                     ADSDAEILAEADALVDAEAEALVLAEADALVLAEADALVDADSEALVDAETEAL"
BASE COUNT          255 a          313 c          223 g          198 t
ORIGIN      
        1 agtgcttcag tctcagcgtc aaccagtgcc tctgaatccg catcaacaag tgcatcggct
       61 tcagcaagca ccagtgcgtc ggcttcagca agtactagcg cctcagcctc agcatcaacc
      121 agtgcatcag cctcagcaag tatctcagcg tctgaatcgg catcaacgag tgcgtcagcc
      181 tcagcaagta ctagtgcatc agcttcagca tcaacaagcg cctcagcttc agcatcaacg
      241 agtgcgtcag cctcagcatc aacaagtgcg tcggcttcgg catcaacaag tgcctcagcc
      301 tcagcaagta caagtgcatc agagtcagca agtacaagtg catcagagtc agcaagtacc
      361 agtgcgtcag cttcagcatc aacgagtgcg tccgcttcag catcaacgag tgcgtcagcc
      421 tcagcaagta ccagtgcatc agagtcagca agtactagcg cctcagcttc agcaagtacc
      481 agcgcatcag tctcagcaag caccagtgct tcagcttcag catcaacgag tgcttcagcc
      541 tcagcaagca ccagtgcttc agcttcagcg tcaacaagtg cttcagcctc agcaagcaca
      601 tcagcgtctg aatctgcatc aacgagtgct tcagcttcag caagcaccag tgcgtcggct
      661 tcagcgtcaa cgagtgcttc ggcttcagca tcgacaagtg cctcagcttc cgcatcaacc
      721 agtgcctcag cttcagcaag taccagcgca tcagtctcag caagcaccag tgcttcagct
      781 tcagcatcaa cgagtgcgtc agcctcagca agtaccagtg cctcggcttc agcatcaacg
      841 agtgcttcag tctcagcgtc aaccagtgcc tctgaatccg catcaacaag tgcatcggct
      901 tcagcaagca ccagtgcatc ggcttcagca agtacaagtg cttcagcttc agcaagtacc
      961 tcagcttctg aatcggcatc aacgagtgc
//
LOCUS       DAVKQL010000136          932 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVKQL010000136 DAVKQL010000000
VERSION     DAVKQL010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.136"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            <1..791
                     /locus_tag="VW561_002108"
     CDS             <1..791
                     /locus_tag="VW561_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001840385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU9944297.1"
                     /translation="FLYMLKKLDKNGFGKVKTVITCHGWVEYNLKKKFLTYFDFWTYS
                     MGDAFICVSETMKKRLESIIKNKKIVAINNGINVSNSDLDVVGVQDFKKEFCISNNKK
                     IICYVGRLDPEKRPDRFLEFAEKLFLVREDVIFIMAGNGSMWAALKEKICHLKYRDNF
                     RLLGEIYPATIVYQISDLLYIPSDTEGIPMCVLESMSQGTPVLASNVGGLSEIIEHRV
                     DGFLFEKEDVEGVCACANFLLNDSEYLKYIGENSKSKIRKHESI"
     gene            815..>932
                     /locus_tag="VW561_002109"
     CDS             815..>932
                     /locus_tag="VW561_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9944298.1"
                     /translation="MDRNIDQELVSIIIPTHNRYESLIRAVKSCLHQSYKNIE"
BASE COUNT          346 a           80 c          195 g          311 t
ORIGIN      
        1 attttttata tatgttaaaa aaacttgaca agaatggatt tggaaaggtt aagacagtga
       61 taacatgcca tggatgggtt gaatataact taaaaaagaa attcttgact tattttgatt
      121 tttggacata ttctatggga gatgctttta tttgtgtatc tgaaactatg aaaaaaagat
      181 tggagagtat aataaaaaat aagaagatag ttgcaataaa taacggaatt aatgtatcaa
      241 atagcgattt agatgtagta ggtgttcaag actttaaaaa agaattttgt atttcaaata
      301 acaaaaaaat catttgttat gttggaaggt tggatccaga aaaaaggccg gatagattcc
      361 ttgaatttgc agaaaaatta tttttagtca gagaagatgt gatttttatt atggctggaa
      421 atggtagtat gtgggctgct ttgaaagaaa aaatttgcca tttgaaatat agagataatt
      481 tcaggttact tggtgaaatc tatccagcta ctatagttta ccaaatatcg gatttattgt
      541 atattccttc tgatacagaa ggaataccta tgtgtgtttt agagtcaatg tcacaaggaa
      601 ctccggtttt agctagtaat gttggcgggt taagtgaaat tattgaacat agggttgatg
      661 gatttttatt tgagaaggaa gatgttgagg gagtgtgtgc ttgtgctaat tttttactca
      721 atgattctga gtatttgaaa tatataggtg agaatagtaa atcaaaaata agaaaacatg
      781 agagtatatg atgaattatt agagaagagt agtcatggat aggaatatag atcaagaatt
      841 agtaagtatt ataataccaa cacataatag gtatgaatct ctgattagag ctgtgaaaag
      901 ttgtttacat caaagttata aaaatatcga ag
//
LOCUS       DAVKQL010000137          886 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVKQL010000137 DAVKQL010000000
VERSION     DAVKQL010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.137"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            102..886
                     /locus_tag="VW561_002110"
                     /pseudo
     CDS             102..886
                     /locus_tag="VW561_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          304 a          157 c          184 g          241 t
ORIGIN      
        1 tgcaaataag aaatctccag attaggaact atccgtgagt tctctagtct ggagattttt
       61 caatagactt cctgcgaaac aaaatatggt ataatagttc tatgaattat gaagcaagta
      121 aacaactaac cgatgcacga tttaagcgtc ttgttggtgt tcaacgcacg acttttgaag
      181 agatgttagc tgtattaaaa acagcttatc aacttaaaca cgcaaaaggt gggcgaaaac
      241 ctaaattaag cctagaagac cttcttatgg ccactcttca atatgtgcga gaatatcgaa
      301 cttatgaaga aattgcggct gatcttggta ttcacgaaag caacttaatc cgtcggagcc
      361 aatgggttga agtaactctt gttcaaaggt ggtgttgcga tttcaagaac tcctctcagt
      421 tctgaggaca cggtaatgat tgatgcgacg gaagtaaaaa tcaatcgacc taaaaaaaga
      481 attagcgaat gattctggta aaaagaaatt tcacgctatg aagactcaag cgattgtcac
      541 aagtcaaggg agaattattt ctttggatat cgctgtgaac tattgtcatg atatgaagtt
      601 gttcaaaatg agtcgcagaa atatcggaca agctggtaaa atcttggctg acagtggtta
      661 tcaagggctc atgaagatat atcttcaagc acaaactcca cgtaaatcca gcaaactcaa
      721 gccgctaaca gctgaagata aagcctgtaa ccatgtgcta tctaaggaga gaagcaaggt
      781 tgagaacatc tttgccaaag taaaaacgct taaaatgttt tcaacaacct atcgaaatca
      841 tcgtaaacgc ttcggattac ggatgaattt gattgttggt attatc
//
LOCUS       DAVKQL010000138          848 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVKQL010000138 DAVKQL010000000
VERSION     DAVKQL010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.138"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            <1..>848
                     /gene="phtD"
                     /locus_tag="VW561_002111"
     CDS             <1..>848
                     /gene="phtD"
                     /locus_tag="VW561_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtD"
                     /protein_id="HEU9944299.1"
                     /translation="RTARGVAVPHGNHYHFIPYEQMSELEKRIARIIPLRYRSNHWVP
                     DSRPEQPSPQPTPEPSPSPQPAPNPQPAPSNPIDEKLVKEAVRKVGDGYVFEENGVSR
                     YIPAKDLSAETAAGIDSKLAKQESLSHKLGAKKTDLPSSDREFYNKAYDLLARIHQDL
                     LDNKGRQVDFEALDNLLERLKDVSSDKVKLVDDILAFLAPIRHPERLGKPNAQITYTD
                     DEIQVAKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTHSHWIKKDSLSEAERAAAQ
                     AYAKEK"
BASE COUNT          273 a          183 c          180 g          212 t
ORIGIN      
        1 tcgaaccgcc agaggtgtag ctgtccctca tggtaaccat taccacttta tcccttatga
       61 acaaatgtct gaattggaaa aacgaattgc tcgtattatt ccccttcgtt atcgttcaaa
      121 ccattgggta ccagattcaa gaccagaaca accaagtcca caaccgactc cggaacctag
      181 tccaagtccg caacctgcac caaatcctca accagctcca agcaatccaa ttgatgagaa
      241 attggtcaaa gaagctgttc gaaaagtagg cgatggttat gtctttgagg agaatggagt
      301 ttctcgttat atcccagcca aggatctttc agcagaaaca gcagcaggca ttgatagcaa
      361 actggccaag caggaaagtt tatctcataa gctaggagct aagaaaactg acctcccatc
      421 tagtgatcga gaattttaca ataaggctta tgacttacta gcaagaattc accaagattt
      481 acttgataat aaaggtcgac aagttgattt tgaggctttg gataacctgt tggaacgact
      541 caaggatgtc tcaagtgata aagtcaagtt agtggatgat attcttgcct tcttagctcc
      601 gattcgtcat ccagaacgtt taggaaaacc aaatgcgcaa attacctaca ctgatgatga
      661 gattcaagta gccaagttgg caggcaagta cacaacagaa gacggttata tctttgatcc
      721 tcgtgatata accagtgatg agggggatgc ctatgtaact ccacatatga cccatagcca
      781 ctggattaaa aaagatagtt tgtctgaagc tgagagagcg gcagcccagg cttatgctaa
      841 agagaaag
//
LOCUS       DAVKQL010000139          813 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVKQL010000139 DAVKQL010000000
VERSION     DAVKQL010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.139"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            323..547
                     /gene="pneA1"
                     /locus_tag="VW561_002112"
     CDS             323..547
                     /gene="pneA1"
                     /locus_tag="VW561_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA1"
                     /protein_id="HEU9944300.1"
                     /translation="MTNFNSNEKFCGKSLKSLSADEMSLIYGASDGAEPRWTPTPIIL
                     KSAAASSKVCISAAVSGIGGLVSYNNDCLG"
     gene            554..742
                     /gene="pneA2"
                     /locus_tag="VW561_002113"
     CDS             554..742
                     /gene="pneA2"
                     /locus_tag="VW561_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA2"
                     /protein_id="HEU9944301.1"
                     /translation="MKNDFVIGKSLKELSLEEMQLVYGGTDGADPRSTIICSATLSFI
                     ASYLGSAQTRCGKDNKKK"
BASE COUNT          287 a          102 c          163 g          261 t
ORIGIN      
        1 aatatatctg ttagaataaa aacaaggaaa aagaaagggg tttcattgca tgagaaaaaa
       61 acgtggaatt aaaaaattag ttacatttgc attgctaggt gtttttatgt ttagtaatac
      121 aattccttac caacagttta ttcagaagaa taaacaattg gagattcgag tgcaatcgca
      181 aaagaagtcc aatggtcttg atgttgggaa ggcggattaa aacccgatat tttgcacgag
      241 aatgagagaa ttataattta tctgaaaaaa cgaaaaatat agagcaactt atttaacttt
      301 ttataaggag gagttattac ttatgaccaa ctttaattca aacgaaaaat tttgtggaaa
      361 aagtttaaaa tcattgtctg ctgatgaaat gagtttaatt tatggtgcat ctgatggtgc
      421 agagccacgt tggacgccta ctcctattat tttgaaatca gctgcagcta gctcgaaagt
      481 atgcattagt gcagctgttt ctggtattgg ggggctagtt agctacaata atgattgttt
      541 ggggtaaatt aaaatgaaaa atgattttgt aatcggaaag agtttgaaag aattatctct
      601 tgaagaaatg caattggttt atggtggcac agatggtgct gaccctagat caacaattat
      661 atgttctgct acattaagtt ttattgcatc atatttgggg tcagctcaga ctcgttgtgg
      721 caaagacaac aagaaaaaat aaaatattca attttaaaaa taaaagttgc tctttatttt
      781 cctctctcgt aactaataat tttgagagag gtt
//
LOCUS       DAVKQL010000140          792 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVKQL010000140 DAVKQL010000000
VERSION     DAVKQL010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.140"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            1..219
                     /locus_tag="VW561_002114"
                     /pseudo
     CDS             1..219
                     /locus_tag="VW561_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819222.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
     gene            273..509
                     /locus_tag="VW561_002115"
     CDS             273..509
                     /locus_tag="VW561_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944302.1"
                     /translation="MNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVKQKL
                     PKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            493..789
                     /locus_tag="VW561_002116"
     CDS             493..789
                     /locus_tag="VW561_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944303.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYKLTEYSKMK"
BASE COUNT          348 a           81 c          139 g          224 t
ORIGIN      
        1 taaccgtttt aaataaccaa ttttataatt tatccggtaa gtatatatca ttcatcaaat
       61 ctaatgcaaa tgggaaagaa cctgttatac gtgatagcga tggtaatttc aatattgtaa
      121 cggagaatgg gctttacaaa attgtaacaa ataatttaag tgataaaaac gaaaaagaaa
      181 aaaacaaaga ggaaaaacaa tataattcaa ataatgtaat tgatagtaac cagaagaacg
      241 gagagtttaa ctcaagtaaa gataatagac aaatgaatga caagatcgac aataaacaag
      301 ataataagac agaagaagta aactataaaa tagttggaga tggcagagaa actgaaaatc
      361 atattaataa atctaaagaa atagtagatg taaaacaaaa attaccaaag actggttcga
      421 acaagattat ggaactattc ttaacagtga caggaattgg tttacttttg acactaaaag
      481 ggttgaagta ttatggtaaa gataaataaa atttgttcga tacaaggaag ttccgtagaa
      541 aatgaggata ttgtaggttc acaaaatcag tatttttgga ttattggtgg agctacagat
      601 ttatataatt ctaaagagga gataggttat tcagtctcag aagttgtaca tattttatca
      661 gaaagtttgt cggttaattg taaagaatca aaaactctta aacaaatatt tgaaactgct
      721 ttactcgagg ttaaagatga aatcggttta aactcatata aattaacaga gtatagtaaa
      781 atgaaataat aa
//
LOCUS       DAVKQL010000141          742 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVKQL010000141 DAVKQL010000000
VERSION     DAVKQL010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.141"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            <1..574
                     /locus_tag="VW561_002117"
     CDS             <1..574
                     /locus_tag="VW561_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9944304.1"
                     /translation="KIPSGLYKIDTGKVLFYGININQIDKKILSQNLGVVPQDSFLLN
                     RSILDNITLKHEVTSQKIEEVCKAVQIYDEIMAMPMKFNTIISEMGSNISGGQRQRIA
                     LARALINNPSIVILDEATSALDTINEERITKYIQSQGCTQIIIAHRLSTIKDADIIFV
                     MKGGKIVESGNHKYLITLGGEYYSLYTKRK"
     gene            587..>742
                     /locus_tag="VW561_002118"
     CDS             587..>742
                     /locus_tag="VW561_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944305.1"
                     /translation="MKKENEYVILTIASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYK"
BASE COUNT          281 a           79 c          148 g          234 t
ORIGIN      
        1 aaaaatacca tcaggattat ataagattga tacaggaaaa gttttatttt atggtataaa
       61 tattaatcaa atagataaaa aaatattaag tcaaaattta ggagtagttc cacaggattc
      121 atttttattg aaccgaagta ttcttgataa tataacttta aagcacgaag ttacttcaca
      181 aaagatagag gaagtttgta aagcagttca aatctatgat gaaatcatgg ctatgccgat
      241 gaaatttaat actatcatct cagagatggg gtcaaatatt tcaggtgggc aaaggcaacg
      301 gatagcactg gcacgtgcat taataaataa tcctagtatt gtaattttag atgaagcaac
      361 tagtgcatta gacactatta atgaggaaag aataacaaag tatatacaaa gtcagggttg
      421 tactcaaata attatagctc atagattgtc aacgattaag gatgcggata ttatttttgt
      481 aatgaaaggt ggtaagattg ttgagtcagg aaatcataag tacttaataa ctcttggtgg
      541 agagtactac agcttatata caaaaaggaa atgaggtgta aagaaaatga agaaagaaaa
      601 tgaatatgta attttaacaa tagcctcact aggggtgatg attggaatag tgtttgcaat
      661 ttttttagat tttccagttg aatatggtat ttctttaggc ttgttgaatg gaatagtatt
      721 gggttcgctg attgtttaca aa
//
LOCUS       DAVKQL010000142          681 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVKQL010000142 DAVKQL010000000
VERSION     DAVKQL010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.142"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            22..363
                     /locus_tag="VW561_002119"
     CDS             22..363
                     /locus_tag="VW561_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0218 family bacteriocin-like peptide"
                     /protein_id="HEU9944306.1"
                     /translation="MIDEEEMMYLDGGAIYIPRWAITGAITGAAYAALAAAGGGGLQL
                     VLASYGLRSALVAGIVKGLGVLGIHIGNAFANTVIRSIASAGIGAGADWIFTNIIDGW
                     DGRRDNQLRIG"
     gene            371..583
                     /locus_tag="VW561_002120"
     CDS             371..583
                     /locus_tag="VW561_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9944307.1"
                     /translation="MMVVLISVIVAFLYHKITEYVNNRKIDIVIFLSLLISHFIWNYY
                     FLPIEILIAISGANLLLSFLKKLQIK"
BASE COUNT          218 a           80 c          145 g          238 t
ORIGIN      
        1 gtattaccaa ataattatgt tgtgattgat gaagaagaga tgatgtacct tgatgggggg
       61 gctatatata tacccaggtg ggcaattaca ggagccatta ctggtgcagc atatgcagca
      121 ttagcagcag caggaggtgg aggccttcaa ctagttcttg catcttatgg attacgctcc
      181 gcactggtag ctgggattgt taaaggttta ggagtattag gaattcatat tggaaatgct
      241 tttgcaaata ctgttattag aagtattgca tctgctggaa ttggtgctgg agctgattgg
      301 atttttacca atattattga tggctgggat gggcgacgtg ataatcaatt gagaatagga
      361 taatagtatt atgatggttg ttttaatttc agttattgtt gcattcctgt accataaaat
      421 tacagagtat gtcaacaaca ggaaaattga tattgttatt tttctatcat tgctgatttc
      481 tcattttatt tggaattact attttttacc aattgaaata ttgatagcaa tcagtggagc
      541 aaatttatta ttaagttttt taaaaaaatt acagattaaa taatattatt ccatatgaca
      601 tatcaactag gtcattagat tttattgaag atataaatta aaaacgaggt tgggaaaaaa
      661 ctcaatttga aagtgaaatt g
//
LOCUS       DAVKQL010000143          487 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVKQL010000143 DAVKQL010000000
VERSION     DAVKQL010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.143"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(<1..>487)
                     /locus_tag="VW561_002121"
     CDS             complement(<1..>487)
                     /locus_tag="VW561_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HEU9944308.1"
                     /translation="LEKSGIFDLLQNHLVSNLVDYVTGVEVGMDTNGRKNRTGDAMEN
                     IVQSYLEAEGYILGENLFKEIEQNEIEEIFSVDLSAITNDGNTVKRFDFVIKNEQVLY
                     LIEVNFYSGSGSKLNETARSYKMIAEEIKAIPNVEFMWITDGQGWYKAKNNLRETFDI
                     LP"
BASE COUNT          167 a          106 c           46 g          168 t
ORIGIN      
        1 ggtaaaatat caaatgtttc tcgtaaatta ttcttagcct tataccaacc ttgtccatca
       61 gtaatccaca taaactcaac attaggaata gctttaattt cttcagcaat cattttgtaa
      121 gatctagccg tctcatttaa ttttgaacca cttcctgaat aaaaattaac ctcaatcaga
      181 taaagcactt gttcattttt tatgacaaaa tcaaaacgtt tcacagtatt cccatcattc
      241 gtaatagcag ataaatcgac agaaaaaatt tcctctattt cattttgttc aatctcttta
      301 aataaattct ctcctaaaat atacccttca gcttcaagat agctttgaac aatattttcc
      361 atcgcatctc cagtccgatt tttgcgccca ttagtatcca taccaacctc aacacctgta
      421 acgtagtcaa ctaaatttga tactaaatga ttttgcaata aatcaaatat acctgatttt
      481 tccaaaa
//
LOCUS       DAVKQL010000144          462 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVKQL010000144 DAVKQL010000000
VERSION     DAVKQL010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.144"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            complement(300..>462)
                     /locus_tag="VW561_002122"
     CDS             complement(300..>462)
                     /locus_tag="VW561_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9944309.1"
                     /translation="TDVIAMAGLTTNVMAQMGKDKPITFKNLERICKALSCTPNDIIS
                     FEDNFSDEE"
BASE COUNT          135 a           83 c           50 g          194 t
ORIGIN      
        1 aaaaaaggat ctagttttta tttttagtta aattatgtct atatttttca ttaactcatc
       61 aataaaaatt tgtttgctaa tatctctttc ttctgatttt gtttcaagga ttattgctac
      121 atcattcata tttttaggta aataccaacc atctggctta tctgaatatc ccttgaagcc
      181 taattgatta aaagtagtta tttgaccagt accttgattg atgttttctt caacttcatt
      241 aaagcctata cttctcttgc ataatcccta acttgatctt ctgtccttaa agtcattttc
      301 tattcctcgt cactaaaatt atcttcaaaa ctaataatat cattaggagt gcaagataaa
      361 gccttacata ttctttctaa attcttaaat gtaattggtt tatcctttcc catttgtgcc
      421 ataacatttg ttgtaagtcc tgccatagct attacatctg tt
//
LOCUS       DAVKQL010000145          442 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVKQL010000145 DAVKQL010000000
VERSION     DAVKQL010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.145"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            <1..>442
                     /locus_tag="VW561_002123"
     CDS             <1..>442
                     /locus_tag="VW561_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU9944310.1"
                     /translation="YLKDAAHADNIRTKEEIKRQKQERSHNHNSRADNAVAAARAQGR
                     YTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNELSASELAAAEAYWNGKQGSRP
                     SSSSSYNANPAQPRLSENHNLTVTPTYHQNQGENISSLLRELYA"
BASE COUNT          142 a           93 c           93 g          114 t
ORIGIN      
        1 tttaccttaa ggatgcagct catgcggata atattcggac aaaagaagag attaaacgtc
       61 agaagcagga acgcagtcat aatcataact caagagcaga taatgctgtt gctgcagcca
      121 gagcccaagg acgttataca acggatgatg ggtatatctt caatgcatct gatatcattg
      181 aggacacggg tgatgcttat atcgttcctc acggcgacca ttaccattac attcctaaga
      241 atgagttatc agctagcgag ttagctgctg cagaagccta ttggaatggg aagcagggat
      301 ctcgtccttc ttcaagttct agttataatg caaatccagc tcaaccaaga ttgtcagaga
      361 accacaatct gactgtcact ccaacttatc atcaaaatca aggggaaaac atttcaagcc
      421 ttttacgtga attgtatgct aa
//
LOCUS       DAVKQL010000146          421 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVKQL010000146 DAVKQL010000000
VERSION     DAVKQL010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.146"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
     gene            1..278
                     /gene="tnpB"
                     /locus_tag="VW561_002124"
                     /pseudo
     CDS             1..278
                     /gene="tnpB"
                     /locus_tag="VW561_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          122 a           73 c           90 g          136 t
ORIGIN      
        1 gtaagacaga tatgaggtag ggaatagact cattagccta tgtagttaaa acccactttg
       61 aattggattc tttctccggt caagtctttc tcttttgtgg tggacgtaaa gaccgcttta
      121 aaacccttta ctgggatggt caaggatttt ggctactata taaacgcttt gagaacggaa
      181 aactgacttg gctaagtaca gaaaaggatg tcaaagctct cgcacctgaa caagtagatt
      241 ggctgatgaa gggcttttct atcactcccc aaatatagta gattgaaact agaatagtac
      301 acctctgctt ctaaaatatt gttagaaatc gatttgactg tcctgatcga tttgtcctgt
      361 tcttgtttcg ttttactata gaacgatttg aaggcgttta tgatatttag ctgtacgaga
      421 g
//
LOCUS       DAVKQL010000147          407 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00062082
            SAMN33606347-rid17946763.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVKQL010000147 DAVKQL010000000
VERSION     DAVKQL010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606347
            Sequence Read Archive: SRR23727241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722283.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 1,952
            CDSs (with protein)               :: 1,952
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 61 of 125
            Pseudo Genes (incomplete)         :: 60 of 125
            Pseudo Genes (internal stop)      :: 48 of 125
            Pseudo Genes (multiple problems)  :: 39 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606347-rid17946763.denovo.147"
                     /strain="AUSMDU00062082"
                     /serotype="17F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST392"
BASE COUNT          155 a           81 c           48 g          123 t
ORIGIN      
        1 gaatttcacc aattcctgca aaaaaatgtt tcatagctat agttttacgc ccataaatac
       61 ccatcaaatt taccgtctac gatatctaaa ccatatgcaa tggctcctcc taatgagaag
      121 acttcagcaa cagtagccgc aatattaaga gcattagtaa tagttgccat actaactcca
      181 ccaatatatc ttgttatagc acttagttac ctagataaga tatcctttag cttgcttcca
      241 aaatttaagt ctacacctat aatatgagtt catattagta ctgctaaatc ctatctatag
      301 taatgttaaa cacctaaata aagtcatctc tccaattcat tcaagaaatg aagaaaggca
      361 aaaaatcttt aaaataagaa aaatgcatat tatcaggtgt tcaaaaa
//
